Research activity information

• Mar. 2022 日本畜産学会, Animal Science Journal Reviewers Award


• Mar. 2020 日本家禽学会, The Journal of Poultry Science Outstanding Paper Award, Role of insulin-like growth factor-1 in the central regulation of feeding behavior in chicks

  Fujita S, Honda K, Yamaguchi M, Fukuzo S, Saneyasu T, Kamisoyama H


• Mar. 2019 Japanese Society of Animal Science, Animal Science Journal Reviewers Award

  HONDA KAZUHISA

  Japan society


• Mar. 2015 Japanese Society of Animal Science, Award, Physiological and biochemical studies on the regulation of nutrient metabolism in chickens

  HONDA KAZUHISA

  Japan society

• Effects of Central Administration of Opioid Peptides, Vasotocin, Mesotocin, and Corticotrophin-Releasing Factor on Water Intake in Chicks

  Yuhui Zhang, Kaoruko Murata, Junya Takegaki, Takaoki Saneyasu, Kazuhisa Honda

  Japan Poultry Science Association, Mar. 2025, The Journal of Poultry Science, 62, jpsa.2025011 - n/a, English

  [Refereed]

  Scientific journal


• Wet Feeding Promotes Growth without Affecting Hypothalamic Peptide Gene Expression in Growing Broiler Chicks

  Tomoya Matsunami, Yuhui Zhang, Yuji Taniguchi, Sei-ichi Hinomoto, Takaoki Saneyasu, Hiroshi Kamisoyama, Kazuhisa Honda

  Japan Poultry Science Association, Feb. 2025, The Journal of Poultry Science, 62, jpsa.2025008 - n/a, English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of Amino acid supplementation to a low-protein diet on the growth performance and protein metabolism-related factors in broiler chicks

  Apr. 2024, El-Far AS;Kamiya M;Saneyasu T;Honda K, 61, jpsa.2024014, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Periodic and local LED light switching induces the locomotion of broilers

  Shingo Kusuda, Chikamitsu Oshima, Takaoki Saneyasu, Kazuhisa Honda

  Mar. 2024, The Journal of Poultry Science, 61, jpsa.2024009, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of dietary brown rice and sake lees on the growth performance and color of meat in broiler chicks

  Koki Nishikawa, Karin Miyazaki, Takehiro Hirai, Takaoki Saneyasu, Kazuhisa Honda

  Japan Poultry Science Association, Mar. 2024, The Journal of Poultry Science, 61, 2024007, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Role of hypothalamic transforming growth factor-β (TGF-β)/Smad signaling in feeding regulation in chickens.

  Takaoki Saneyasu, Miku Ueda, Kanami Nagata, Jiawei Chai, Kazuhisa Honda, Hiroshi Kamisoyama

  Previous studies in mammalian obesity models have suggested that central transforming growth factor-β (TGF-β) controls the gene expression of appetite-regulating neuropeptides and peripheral energy metabolism. In the present study, we investigated the possible involvement of central TGF-β/Smad signaling in feeding regulation in chickens. Central administration of TGF-β1 resulted in phosphorylation of Smad2 in the hypothalamus of chicks and suppressed feed intake without changing the gene expression of hypothalamic appetite-regulating neuropeptides (neuropeptide Y, agouti-related protein, proopiomelanocortin, and corticotropin-releasing factor). However, neither fasting nor refeeding induced the phosphorylation of hypothalamic Smad2. These findings suggest that the activation of hypothalamic TGF-β/Smad signaling suppresses feed intake in chicks but it might not occur in response to feeding status.

  Oct. 2022, The journal of poultry science, 59(4) (4), 357 - 363, English, Domestic magazine

  Scientific journal


• Atrogin-1 knockdown inhibits the autophagy-lysosome system in mammalian and avian myotubes

  Takaoki Saneyasu, Kazuki Ogasawara, Yuki Fujiwara, Kazuhisa Honda, Hiroshi Kamisoyama

  Atrogin-1 plays an important role in ubiquitin-proteasome proteolysis in vertebrate skeletal muscles. Recently, atrogin-1 has been shown to be involved in the autophagy-lysosome system, another proteolytic system, in the murine and fish hearts and skeletal muscles. With the aim to elucidate the effect of atrogin-1 on the autophagy-lysosome system in mammalian and avian skeletal muscles, this study has examined the effects of atrogin-1 knockdown on autophagy-lysosome-related proteins in C2C12 and chicken embryonic myotubes. Using the levels of microtubule-associated protein light chain 3 (LC3)-II protein, it was confirmed that atrogin-1 knockdown blocked the autophagic flux in both the myotubes. In addition, atrogin-1 knockdown in C2C12 myotubes significantly decreased the level of autophagy-related gene (ATG)12-ATG5 conjugate, which is supposedly necessary for the fusion of autophagosomes and lysosomes. Atrogin-1 knockdown also resulted in downregulation of forkhead box O3, a transcription factor for ATG12. These data suggest that atrogin-1 is essential for the normal autophagy-lysosome system in the striated muscles of vertebrates.

  Elsevier BV, Sep. 2022, Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 271, 111262 - 111262, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• IGF-1 knockdown inhibits phosphorylation of Akt and ERK in chicken embryonic myotubes

  Takaoki Saneyasu, Tomonori Nakamura, Kazuhisa Honda, Hiroshi Kamisoyama

  Elsevier BV, Aug. 2022, Growth Hormone & IGF Research, 65, 101478 - 101478, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Comparison of the effects of intracerebroventricular administration of glucagon-like peptides 1 and 2 on hypothalamic appetite regulating factors and sleep-like behavior in chicks

  Ahmed Kewan, Tomohiko Shimatani, Takaoki Saneyasu, Hiroshi Kamisoyama, Kazuhisa Honda

  Elsevier BV, Jan. 2022, Neuroscience Letters, 768, 136362 - 136362, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Role of corticosterone in lipid metabolism in broiler chick white adipose tissue

  Kazuhisa Honda, Kiyotaka Kurachi, Shoko Takagi, Takaoki Saneyasu, Hiroshi Kamisoyama

  Japan Poultry Science Association, 2022, The Journal of Poultry Science, 59(2) (2), 152 - 158, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Enzymatically-synthesized glycogen induces cecal glucagon-like peptide-1 production and suppresses food intake in mice

  Kazuhisa Honda, Aki Yasuhara, Takaoki Saneyasu, Hiroshi Kamisoyama

  Aug. 2021, Journal of Nutritional Science and Vitaminology, 67(4) (4), 217 - 224, English

  [Refereed]

  Scientific journal


• Central administration of insulin and refeeding lead to Akt and ERK phosphorylation in the chicken medulla

  Takaoki Saneyasu, Mizuki Ueno, Kanami Nagata, Ahmed Kewan, Kazuhisa Honda, Hiroshi Kamisoyama

  Elsevier BV, Jun. 2021, Neuroscience Letters, 136008 - 136008

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Central administration of insulin-like growth factor-2 suppresses food intake in chicks

  Kazuhisa Honda, Ahmed Kewan, Haruki Osada, Takaoki Saneyasu, Hiroshi Kamisoyama

  Elsevier BV, Apr. 2021, Neuroscience Letters, 751, 135797 - 135797, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of fasting and re-feeding on the expression of CCK, PYY, hypothalamic neuropeptides, and IGF-related genes in layer and broiler chicks

  Ahmed Kewan, Takaoki Saneyasu, Hiroshi Kamisoyama, Kazuhisa Honda

  Corresponding, Elsevier BV, Mar. 2021, Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 257, 110940 - 110940, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Role of insulin-like growth factor-1 in the central regulation of feeding behavior in chicks

  Shoichi Fujita, Kazuhisa Honda, Mika Yamaguchi, Satoshi Fukuzo, Takaoki Saneyasu, Hiroshi Kamisoyama

  Corresponding, Japan Poultry Science Association, Nov. 2019, The Journal of Poultry Science, 56(4) (4), 270 - 276, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Myostatin increases Smad2 phosphorylation and atrogin-1 expression in chick embryonic myotubes

  Takaoki Saneyasu, Kazuhisa Honda, Hiroshi Kamisoyama

  Jul. 2019, The Journal of Poultry Science, 56(3) (3), 224 - 230, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Differential regulation of protein synthesis by skeletal muscle type in chickens

  Takaoki Saneyasu, Yuma Nakano, Nami Tsuchii, Tatsuya Tsuchihashi, Sayaka Kimura, Kazuhisa Honda, Hiroshi Kamisoyama

  2019, General and Comparative Endocrinology, 284, English

  [Refereed]

  Scientific journal


• Central administration of insulin and refeeding lead to the phosphorylation of AKT, but not FOXO1, in the hypothalamus of broiler chicks

  Takaoki Saneyasu, Satoshi Fukuzo, Ayana Kitashiro, Kanami Nagata, Kazuhisa Honda, Hiroshi Kamisoyama

  2019, Physiology & Behavior, 210, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of fasting and refeeding on the mRNA levels of insulin-like growth factor-binding proteins in chick liver and brain

  Shoichi Fujita, Mika Yamaguchi, Daichi Hiramoto, Takaoki Saneyasu, Kazuhisa Honda, Hiroshi Kamisoyama

  Corresponding, Japan Poultry Science Association, Nov. 2018, The Journal of Poultry Science, 55(4) (4), 269 - 273, English

  [Refereed]


• The extract of soybean protein increases slow-myosin heavy chain expression in C2C12 myotubes

  Takaoki Saneyasu, Haruka Shindo, Kazuhisa Honda, Hiroshi Kamisoyama

  Aug. 2018, JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, 64(4) (4), 296 - 300, English

  [Refereed]


• Hypothalamic Akt-mediated signaling regulates food intake in chicks

  Takaoki Saneyasu, Shoichi Fujita, Ayana Kitashiro, Satoshi Fukuzo, Kazuhisa Honda, Hiroshi Kamisoyama

  The central anorexigenic mechanism seems to be similar in mammals and chicks, because the appetite-suppressive action of a number of peptide hormones is similar in both species. Accumulating evidence in mammals has revealed that hypothalamic Akt-mediated signaling factors (for instance, mTOR and FOXO1) are significantly involved in the regulation of food intake. However, the role of hypothalamic Akt in feeding regulation is yet to be determined in chickens. In this study, we showed that pAkt (Thr308)/Akt, pFOXO1/FOXO1, and pS6 levels were significantly increased in the hypothalami of chicks refed 1 h after a 24 h-fast in correlation to increases in the plasma concentrations of insulin, one of the activators of the Akt-mediated signaling pathways. In addition, central administration of insulin increased the phosphorylation of Akt, FOXO1, and S6 in chicken hypothalami. Furthermore, intracerebroventricular injections of both phosphoinositide 3-kinase inhibitor LY294002 and mTOR inhibitor rapamyacin enhanced the food intake of chicks. These findings suggest that hypothalamic Akt-mediated signaling pathways contribute to the regulation of food intake in chicks.

  Elsevier Ireland Ltd, Mar. 2018, Neuroscience Letters, 670, 48 - 52, English

  [Refereed]

  Scientific journal


• Effects of enzymatically synthesized glycogen on lipid metabolism in diet induced obese mice.

  FURUYASHIKI Takashi, OGAWA Rui, NAKAYAMA Yoko, HONDA Kazuhisa, KAMISOYAMA Hiroshi, TAKATA Hiroki, YASUDA Michiko, KURIKI Takashi, ASHIDA Hitoshi

  2018, Food Science and Technology Research, 24, 119 - 127, English

  [Refereed]

  Scientific journal


• Effects of enzymatically synthesized glycogen on lipid metabolism in diet induced obese mice

  Takashi Furuyashiki, Rui Ogawa, Yoko Nakayama, Kazuhisa Honda, Hiroshi Kamisoyama, Hiroki Takata, Hiroshi Kamasaka, Michiko Yasuda, Takashi Kuriki, Hitoshi Ashida

  Jan. 2018, Food Science and Technology Research, 24(1) (1), 119 - 127, English

  [Refereed]

  Scientific journal


• 夜間における青色照明の点灯がブロイラーの行動，時計遺伝子発現，及び視床下部-下垂体-副腎皮質軸に及ぼす影響

  平本 大地, HONDA KAZUHISA, 近藤 真, 實安 隆興, 上曽山 博

  Corresponding, Nov. 2017, 日本家禽学会誌, 54(J2) (J2), J37 - J43, Japanese

  [Refereed]

  Scientific journal


• The IGF-1/Akt/S6 pathway and expressions of glycolytic myosin heavy chain isoforms are upregulated in chicken skeletal muscle during the first week after hatching

  Takaoki Saneyasu, Tatsuya Tsuchihashi, Ayana Kitashiro, Nami Tsuchii, Sayaka Kimura, Kazuhisa Honda, Hiroshi Kamisoyama

  Skeletal muscle mass is an important trait in the animal industry. We previously reported an age-dependent downregulation of the insulin-like growth factor 1 (IGF-1)/Akt/S6 pathway, major protein synthesis pathway, in chicken breast muscle after 1week of age, despite a continuous increase of breast muscle weight. Myosin heavy chain (HC), a major protein in muscle fiber, has several isoforms depending on chicken skeletal muscle types. HC I (fast-twitch glycolytic type) is known to be expressed in adult chicken breast muscle. However, little is known about the changes in the expression levels of protein synthesis-related factors and HC isoforms in perihatching chicken muscle. In the present study, protein synthesis-related factors, such as IGF-1 messenger RNA (mRNA) levels, phosphorylation of Akt, and phosphorylated S6 content, increased in an age-dependent manner after post-hatch day (D) 0. The mRNA levels of HC I, III and V (fast-twitch glycolytic type) dramatically increased after D0. The increase ratio of breast muscle weight was approximately 1100% from D0 to D7. To our knowledge, these findings provide the first evidence that upregulation of protein synthesis pathway and transcription of fast twitch glycolytic HC isoforms play critical roles in the increase of chicken breast muscle weight during the first week after hatching.

  WILEY, Nov. 2017, ANIMAL SCIENCE JOURNAL, 88(11) (11), 1779 - 1787, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of short-term fasting on the Akt-mediated pathway involved in protein metabolism in chicken skeletal muscle

  T. Saneyasu, N. Tsuchii, Y. Nakano, A. Kitashiro, T. Tsuchihashi, H. Shindo, K. Honda, H. Kamisoyama

  In the present study, we show that short-term (4 h) fasting significantly decreased the levels of protein synthesis-related factors such as the plasma insulin concentration, skeletal muscle pAkt, and pS6 levels in 2-wk-old chickens (P < 0.05). An intravenous injection of insulin significantly elevated the contents of pAkt and p-S6 in the skeletal muscle (P < 0.01). These findings suggest that decreasing the plasma insulin causes the down regulation of the Akt/S6 pathway in chicken skeletal muscle under short-term fasting conditions. However, protein synthesis was not significantly affected by short-term fasting. In addition, no significant change was observed in the levels of proteolysis-related factors such as plasma N-tau-methylhistidine, phosphorylated forkhead box class 0, and muscle ring finger-1 during, 4-h fasting, indicating that short-term fasting does not induce skeletal muscle proteolysis in chickens. Interestingly, atrogin-1 expression significantly increased after 2-h fasting (P < 0.05), and insulin injection significantly reversed the fasting-induced atrogin-1 expression in chicken skeletal muscle (P < 0.01). Collectively, these findings suggest that short-term fasting downregulates the insulin-stimulated Alct/S6 pathway but does not significantly affect protein synthesis and proteolysis in chicken skeletal muscle, and that atrogin-1 expression is upregulated in a FOX01-independent manners. (C) 2017 Elsevier Inc. All rights reserved.

  ELSEVIER SCIENCE INC, Oct. 2017, DOMESTIC ANIMAL ENDOCRINOLOGY, 61(C) (C), 54 - 61, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Central and and peripheral administrations of insulin-like growth factor-1 suppress food intake in chicks

  Shoichi Fujita, Kazuhisa Honda, Daichi Hiramoto, Mei Gyu, Mika Okuda, Shungo Nakayama, Mika Yamaguchi, Takaoki Saneyasu, Hiroshi Kamisoyama

  A number of studies have been made on the physiological actions of insulin-like growth factor-1 (IGF-1) in mammals and birds. In mammals, the effects of central administration of IGF-1 on food intake have been examined. For example, intracerebroventricular administration of IGF-1 significantly decreased food intake in diabetic rats, but not in sheep and nondiabetic rats. The chicken is known to be a hyperglycemic animal. Like satiety hormones, plasma IGF-1 levels are elevated postprandially in chickens. In this study, we hypothesized that IGF-1 is involved in the regulation of food intake in chickens. Intracerebroventricular administration of IGF-1 significantly suppressed food intake in chicks in a dose dependent manner. Both the mRNAs of IGF-1 and its receptor were expressed throughout the brain. However, the mRNA levels of IGF-1 were not influenced by fasting and refeeding in all regions of the brain. On the other hand, 6 h of fasting significantly suppressed mRNA expression of hepatic IGF-1, and this effect was significantly reversed by 6 h of refeeding. Furthermore, in-vascular administration of IGF-1 significantly suppressed food intake in chicks. These findings suggest that IGF-1 may function as a satiety hormone in chickens.

  Corresponding, PERGAMON-ELSEVIER SCIENCE LTD, Oct. 2017, PHYSIOLOGY & BEHAVIOR, 179, 308 - 312, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of dietary heme iron and exercise training on abdominal fat accumulation and lipid metabolism in high-fat diet-fed mice

  Masanori Katsumura, Shoko Takagi, Hana Oya, Shohei Tamura, Takaoki Saneyasu, Kazuhisa Honda, Hiroshi Kamisoyama

  Animal by-products can be recycled and used as sources of essential nutrients. Water-soluble heme iron (WSHI), a functional food additive for supplementing iron, is produced by processing animal blood. In this study, we investigated the effects of dietary supplementation of 3% WSHI and exercise training for 4 weeks on the accumulation of abdominal fat and lipid metabolism in mice fed high-fat diet. Exercise-trained mice had significantly less perirenal adipose tissue, whereas WSHI-fed mice tended to have less epididymal adipose tissue. In addition, total weight of abdominal adipose tissues was significantly decreased in the Exercise + WSHI group. Dietary WSHI significantly increased the messenger RNA (mRNA) levels of lipoprotein lipase and hormone-sensitive lipase. WSHI-fed mice also tended to show increased mRNA levels of adipose triglyceride lipase in their epididymal adipose tissue. Dietary WSHI also significantly decreased the mRNA levels of fatty acid oxidation-related enzymes in the liver, but did not influence levels in the Gastrocnemius muscle. Exercise training did not influence the mRNA levels of lipid metabolism-related enzymes in the epididymal adipose tissue, liver or the Gastrocnemius muscle. These findings suggest that the accumulation of abdominal fat can be efficiently decreased by the combination of dietary WSHI and exercise training in mice fed high-fat diet.

  Corresponding, WILEY, Aug. 2017, ANIMAL SCIENCE JOURNAL, 88(8) (8), 1100 - 1106, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of continuous white light and 12 h white-12 h blue light-cycles on the expression of clock genes in diencephalon, liver, and skeletal muscle in chicks

  Kazuhisa Honda, Makoto Kondo, Daichi Hiramoto, Takaoki Saneyasu, Hiroshi Kamisoyama

  The core circadian clock mechanism relies on a feedback loop comprised of clock genes, such as the brain and muscle Arnt-like 1 (Bmal1), chriptochrome 1 (Cry1), and period 3 (Per3). Exposure to the light-dark cycle synchronizes the master circadian clock in the brain, and which then synchronizes circadian clocks in peripheral tissues. Birds have long been used as a model for the investigation of circadian rhythm in human neurobiology. In the present study, we examined the effects of continuous light and the combination of white and blue light on the expression of clock genes (Bmal1, Cry1, and Per3) in the central and peripheral tissues in chicks. Seventy two day-old male chicks were weighed, allocated to three groups and maintained under three light schedules: 12 h white light-12 h dark-cycles group (control); 24 h white light group (WW group); 12 h white light-12 h blue light-cycles group (WB group). The mRNA levels of clock genes in the diencephalon were significantly different between the control and WW groups. On the other hand, the alteration in the mRNA levels of clock genes was similar between the control and WB groups. Similar phenomena were observed in the liver and skeletal muscle (biceps femoris). These results suggest that 12 h white-12 h blue light-cycles did not disrupt the circadian rhythm of clock gene expression in chicks. (C) 2017 Elsevier Inc. All rights reserved.

  ELSEVIER SCIENCE INC, May 2017, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 207, 73 - 78, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Gut Hormones and Regulation of Food Intake in Birds

  Kazuhisa Honda, Takaoki Saneyasu, Hiroshi Kamisoyama

  Gut hormones act as appetite regulatory hormones in mammals. For example, the hunger hormone ghrelin, which is released from the stomach before food intake, stimulates appetite. In contrast, satiety hormones such as cholecystokinin, glucagon-like peptide-1, and peptide YY, which are released from the intestines after food intake, suppress appetite. The effects of these peptides on food intake have been shown to be similar in both mammals and fishes. However, evidence suggests that the physiological roles of these gut hormones may be different between birds and other vertebrates. This review summarizes the current information on the roles of gut hormones in the regulation of food intake in birds, especially in chickens.

  JAPAN POULTRY SCIENCE ASSOC, Apr. 2017, JOURNAL OF POULTRY SCIENCE, 54(2) (2), 103 - 110, English

  [Refereed][Invited]

  Scientific journal


• Identification, expression analysis, and functional characterization of peptide YY in chickens (Gallus gallus domesticus)

  Koji Aoki, Makoto Kondo, Mika Okuda, Takaoki Saneyasu, Honda Kazuhisa, Hiroshi Kamisoyama

  Corresponding, Elsevier, Jan. 2017, General and Comparative Endocrinology, 242, 11 - 17, English

  [Refereed]

  Scientific journal


• Effects of enzymatically synthesized glycogen and exercise on abdominal fat accumulation in high-fat diet-fed mice

  Shohei Tamura, Kazuhisa Honda, Ryoji Morinaga, Takaoki Saneyasu, Hiroshi Kamisoyama

  The combination of diet and exercise is the first choice for the treatment of obesity and metabolic syndrome. We previously reported that enzymatically synthesized glycogen (ESG) suppresses abdominal fat accumulation in obese rats. However, the effect of the combination of ESG and exercise on abdominal fat accumulation has not yet been investigated. Our goal in this study was therefore to evaluate the effects of dietary ESG and its combination with exercise on abdominal fat accumulation in high-fat diet (HFD)-fed mice. Male ICR mice were assigned to four groups: HFD, HFD containing 20% ESG, HFD with exercise, HFD containing 20% ESG with exercise. Treadmill exercise was performed for 3 wk (25 m/ min, 30 min/d, 3 d/wk) after 5-d adaption to running at that speed. Both ESG and exercise significantly reduced the weights of abdominal adipose tissues. In addition, the combination of ESG and exercise significantly suppressed abdominal fat accumulation, suggesting that ESG and exercise showed an additive effect. Exercise significantly increased the mRNA levels of lipid metabolism-related genes such as lipoprotein lipase, peroxisome proliferator-acti-vated receptor delta factor-delta (PPARδ), carnitin palmitoyltransferase b, adipose triglyceride lipase (ATGL), and uncoupling protein-3 in the gastrocnemius muscle. On the other hand, dietary ESG significantly decreased the mRNA levels of PPARd and ATGL in the gastrocnemius muscle. These results suggest that the combined treatment of ESG and exercise effectively suppresses abdominal fat accumulation in HFD-fed mice by different mechanisms.

  Corresponding, Center for Academic Publications Japan, 2017, Journal of Nutritional Science and Vitaminology, 63(6) (6), 405 - 411, English

  [Refereed]

  Scientific journal


• Fasting and glucagon stimulate gene expression of pyruvate dehydrogenase kinase 4 in chickens

  Kazuhisa Honda, Shoko Takagi, Kiyotaka Kurachi, Haruka Sugimoto, Takaoki Saneyasu, Hiroshi Kamisoyama

  The excessive accumulation of body fat has become a serious problem in the broiler industry. However, the molecular mechanisms underlying the regulation of lipid metabolism-related genes in broiler chickens are not fully understood. In the present study, we investigated the role of glucagon on the expression of lipid metabolism-related genes in chicken white adipose tissue (WAT). Four hours of fasting significantly increased plasma levels of free fatty acid in broiler chickens. The mRNA levels of adipose triglyceride lipase (ATGL) and pyruvate dehydrogenase kinase 4 (PDK4) in abdominal WAT significantly increased by fasting, whereas the mRNA levels of diacylglycerol O-acyl-transferase homolog 2 (DGAT2) and peroxisome proliferator-activated receptor-γ (PPARγ) significantly decreased. The results suggest that fasting stimulates lipolysis and suppresses adipogenesis and re-esterification of TG in chicken WAT. Glucagon significantly increased the mRNA levels of PDK4 in chicken primary adipocytes, whereas there were no significant changes in the mRNA levels of ATGL, DGAT2, and PPARγ. Our findings suggest that glucagon upregulates PDK4 expression and may stimulate lipolysis without affecting the expression of ATGL in chicken WAT.

  Japan Poultry Science Association, 2017, Journal of Poultry Science, 54(4) (4), 292 - 295, English

  [Refereed]

  Scientific journal


• Glucagon-related peptides and the regulation of food intake in chickens

  Kazuhisa Honda

  The regulatory mechanisms underlying food intake in chickens have been a focus of research in recent decades to improve production efficiency when raising chickens. Lines of evidence have revealed that a number of brain-gut peptides function as a neurotransmitter or peripheral satiety hormone in the regulation of food intake both in mammals and chickens. Glucagon, a 29 amino acid peptide hormone, has long been known to play important roles in maintaining glucose homeostasis in mammals and birds. However, the glucagon gene encodes various peptides that are produced by tissue-specific proglucagon processing: glucagon is produced in the pancreas, whereas oxyntomodulin (OXM), glucagon-like peptide (GLP)-1 and GLP-2 are produced in the intestine and brain. Better understanding of the roles of these peptides in the regulation of energy homeostasis has led to various physiological roles being proposed in mammals. For example, GLP-1 functions as an anorexigenic neurotransmitter in the brain and as a postprandial satiety hormone in the peripheral circulation. There is evidence that OXM and GLP-2 also induce anorexia in mammals. Therefore, it is possible that the brain-gut peptides OXM, GLP-1 and GLP-2 play physiological roles in the regulation of food intake in chickens. More recently, a novel GLP and its specific receptor were identified in the chicken brain. This review summarizes current knowledge about the role of glucagon-related peptides in the regulation of food intake in chickens.

  WILEY, Sep. 2016, ANIMAL SCIENCE JOURNAL, 87(9) (9), 1090 - 1098, English

  [Refereed][Invited]

  Link to Kobe Univ. Repository (Kernel)


• The IGF-1/Akt/S6 Signaling Pathway is Age-Dependently Downregulated in the Chicken Breast Muscle

  Takaoki Saneyasu, Mariko Inui, Sayaka Kimura, Yu Yoshimoto, Nami Tsuchii, Haruka Shindo, Kazuhisa Honda, Hiroshi Kamisoyama

  The skeletal muscle mass is known to be controlled by the balance between protein synthesis and degradation. The fractional rate of protein synthesis has been reported to decrease age-dependently from 1 to 4 weeks of age in the chicken breast muscle (pectoralis major muscle). On the other hand, age-dependent change of the fractional protein degradation rate was reported to be less in the skeletal muscle of chickens. These findings suggest that protein synthesis is age-dependently downregulated in chicken muscle. We herein investigated the age-dependent changes in protein synthesis or proteolysis-related factors in the breast muscle of 7, 14, 28, and 49-day old broiler chickens. IGF-1 mRNA level, phosphorylation rate of Akt, and phospho-S6 content were coordinately decreased in an age dependent manner, suggesting that IGF-1-stimulated protein synthesis is downregulated with age in chicken breast muscle. In contrast, atrogin-1, one of the proteolysis-related factors, gradually increased with age at mRNA levels. However, plasma N-tau-methylhistidine concentration, an indicator of skeletal muscle proteolysis, did not coordinately change with atrogin-1 mRNA levels. Taken together, our results suggest that the IGF-1/Akt/S6 signaling pathway is age-dependently downregulated in the chicken breast muscle.

  JAPAN POULTRY SCIENCE ASSOC, Jul. 2016, JOURNAL OF POULTRY SCIENCE, 53(3) (3), 213 - 219, English

  [Refereed]

  Scientific journal


• Role of peroxisome proliferator-activated receptor alpha in the expression of hepatic fatty acid oxidation-related genes in chickens

  Kazuhisa Honda, Takaoki Saneyasu, Haruka Sugimoto, Kiyotaka Kurachi, Shoko Takagi, Hiroshi Kamisoyama

  Liver is the most important target organ for investigation of lipid metabolism in domestic fowls. However, little is known about the regulatory mechanism of fatty acid oxidation in chicken liver. In mammals, proliferator-activated receptor alpha (PPAR alpha), a transcription factor, plays an essential role in the regulation of hepatic fatty acid oxidation. The aim of the present study was to investigate the regulatory mechanisms of PPAR alpha-induced gene expression involved in hepatic fatty acid oxidation in chickens in vivo and in vitro. WY14643, a PPAR alpha agonist, significantly increased the messenger RNA (mRNA) levels of carnitine palmitoyltransferase 1a (CPT1a) and acyl-coenzyme A oxidase (ACO), but not long-, middle-and short-chain acyl-coenzyme A dehydrogenase (LCAD, MCAD and SCAD, respectively), hydroxyacyl-coenzyme A dehydrogenase (HAD), and PPARa itself in chicken hepatoma cells. In contrast, WY14643 significantly increased the mRNA levels of CPT1a, ACO, MCAD, SCAD, HAD and PPAR alpha in human hepatoma cells. The mRNA levels of CPT1a and ACO in the liver were significantly increased by 6 h of fasting in chickens, whereas the mRNA levels of LCAD, MCAD, SCAD and HAD were unchanged. These results suggest that, unlike in mammals, CPT1a and ACO might play an important role in PPAR alpha-induced fatty acid oxidation in the liver of chickens.

  WILEY-BLACKWELL, Jan. 2016, ANIMAL SCIENCE JOURNAL, 87(1) (1), 61 - 66, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Differential regulation of the expression of lipid metabolism-related genes with skeletal muscle type in growing chickens

  Takaoki Saneyasu, Sayaka Kimura, Ayana Kitashiro, Nami Tsuchii, Tatsuya Tsuchihashi, Mariko Inui, Kazuhisa Honda, Hiroshi Kamisoyama

  The regulatory mechanisms of carbohydrate and lipid metabolism are known to differ among skeletal muscle types in mammals. For example, glycolytic muscles prefer glucose as an energy source, whereas oxidative muscles prefer fatty adds (FA). We herein demonstrated differences in the expression of genes involved in carbohydrate and lipid metabolism in the pectoralis major (a glycolytic twitch muscle), adductor superficialis (an oxidative twitch muscle), and adductor profound (a tonic muscle) of 14-day-old chicks. Under ad libitum feeding conditions, the mRNA levels of muscle type phosphofructokinase-1 were markedly lower in the adductor superficialis muscle, suggesting that basal glycolytic activity is very low in this type of muscle. In contrast, high mRNA levels of lipoprotein lipase (LPL) and fatty acid translocase/cluster of differentiation 36 (FAT/CD36) in the adductor superficialis muscle suggest that FA uptake is high in this type of muscle. The mRNA levels of adipose triglyceride lipase (ATGL) and camitine palmitoyltransferase 1b (CPT1b) were significantly higher in the adductor profound muscle than in other muscles, suggesting that basal lipolytic activity is high in this type of muscle. Furthermore, the mRNA levels of peroxisome proliferator activated receptor 6 and CPT1b were significantly increased in the adductor superficialis muscle, but not in other muscles, after 24 h of fasting. Therefore, the availability of FA in the oxidative twitch muscles in growing chickens appears to be upregulated by fasting. Our results suggest that lipid metabolism-related genes are upregulated under both basal and fasting conditions in the adductor superficialis in growing chickens. (C) 2015 Elsevier Inc. All rights reserved.

  ELSEVIER SCIENCE INC, Nov. 2015, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 189, 1 - 5, English

  [Refereed]

  Scientific journal


• Glucagon and Neuromedin U Suppress Food Intake in Broiler Chicks

  Kazuhisa Honda, Takaoki Saneyasu, Mika Okuda, Taku Uemura, Hiroshi Kamisoyama

  Several peptides show different effects on food intake between broiler and layer chicks. For example, the central administration of R-melanocyte-stimulating hormone decreased food intake in layer chicks but not in broiler chicks. The central administration of agouti-related protein increased food intake in layer chicks but not in broiler chicks. We previously reported that glucagon and neuromedin U (NMU) suppress food intake in layer chicks when administered centrally. However, the effects of these peptides on food intake in broiler chicks have not been examined. In the present study, we thus examined whether these peptides also show anorexigenic effects in broiler chicks. The intracerebroventricular administration of glucagon significantly suppressed food intake and increased plasma glucose concentration in broiler chicks. The hyperglycemic effect of glucagon had been shown in layer chicks in our previous studies. Intracerebroventricular administration of NMU significantly suppressed food intake and induced wing-flapping behavior in broiler chicks as well as in layer chicks. All our findings demonstrate that glucagon and NMU function as anorxigenic peptides in both broiler and layer chicks.

  JAPAN POULTRY SCIENCE ASSOC, Oct. 2015, JOURNAL OF POULTRY SCIENCE, 52(4) (4), 268 - 273, English

  [Refereed]

  Scientific journal


• Differences in the expression of genes involved in skeletal muscle proteolysis between broiler and layer chicks during food deprivation

  Takaoki Saneyasu, Sayaka Kimura, Mariko Inui, Yu Yoshimoto, Kazuhisa Honda, Hiroshi Kamisoyama

  Genetic selection results in a higher growth rate and meat yield in broiler chickens than in layer chickens. We herein demonstrated differences in the effects of 24 h of fasting on the expression of genes involved in skeletal muscle proteolysis between broiler and layer chicks. The mRNA levels of proteolysis-related genes were analyzed in the pectoralis major muscle of 14-day-old chicks after 0 or 24 h of fasting. The mRNA levels of ubiquitin ligases such as atrogin-1 and muscle RING finger-1 (MuRF-1) as well as transcription factor forkhead box class O (FOXO) 1 were significantly increased by fasting in broiler and layer chicks, suggesting that the FOXO1-induced ubiquitin-proteasome system, a major proteolytic system in skeletal muscles, was activated by fasting in both chicks. The mRNA levels of atrogin-1 were significantly lower in broiler chicks than in layer chicks after fasting. Furthermore, the mRNA levels of insulin-like growth factor-1 were significantly decreased by fasting in layer chicks, but not in broiler chicks. The mRNA levels of FOXO3 were significantly increased by fasting in layer chicks, but not in broiler chicks. Therefore, the ubiquitin-proteasome system did not appear to have been fully upregulated in broiler chicks. These results suggest that differences in the expression of genes related to the ubiquitin-proteasome system in skeletal muscle proteolysis between broiler and layer chicks during food deprivation are one of the causes of the high growth rate in broiler chickens. (C) 2015 Elsevier Inc. All rights reserved.

  ELSEVIER SCIENCE INC, Aug. 2015, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 186, 36 - 42, English

  [Refereed]

  Scientific journal


• Glucagon-like Peptide-2 Functions as an Anorexigenic Peptide not only in the Central Nervous System but also in the Peripheral Circulation in Broiler Chicks

  Kazuhisa Honda, Tomohiko Shimatani, Koji Aoki, Takuya Yamaguchi, Makoto Kondo, Takaoki Saneyasu, Hiroshi Kamisoyama

  Brain-gut peptides play important roles in the appetite regulatory system in mammals. Glucagon-like peptide (GLP)-1, GLP-2, and oxyntomodulin (OXM) are processed from the same precursor, proglucagon, in both brain and intestines in mammals and birds. We previously showed that intracerebroventricular administration of these three peptides significantly suppressed food intake in chicks. However, peripheral roles of chicken GLP-2 have not yet been investigated, although GLP-2 plays important roles both in central and peripheral regulation of food intake in mammals. The aim of this study is to investigate whether GLP-2 functions as an anorexigenic peptide in both brain and peripheral circulation in chicks. Intracerebroventricular administration of GLP-2 significantly suppressed food intake in chicks. Twenty-four hours of fasting significantly decreased the mRNA level of proglucagon in the medulla oblongata of chicks. These results suggest that GLP-2 functions as anorexigenic peptides in the central nervous system in chicks. In addition, intravenous administration of GLP-2 significantly suppressed food intake in chicks. Lines of evidence suggest that dietary nutrients stimulate the secretion of GLP-2 from L cells in the small intestine in chickens. These findings suggest that GLP-2 functions as both central and peripheral anorexigenic signals in chicks.

  JAPAN POULTRY SCIENCE ASSOC, Jul. 2015, JOURNAL OF POULTRY SCIENCE, 52(3) (3), 183 - 187, English

  [Refereed]

  Scientific journal


• Correlation analysis of hypothalamic mRNA levels of appetite regulatory neuropeptides and several metabolic parameters in 28-day-old layer chickens

  Kazuhisa Honda, Takaoki Saneyasu, Koji Aoki, Tomohiko Shimatani, Takuya Yamaguchi, Hiroshi Kamisoyama

  Various lines of evidence suggest that appetite-related neuropeptides in the hypothalamus are regulated by adiposity signals such as leptin and insulin in mammals. In the present study, we examined age-dependent changes in the weight of abdominal fat and hypothalamic mRNA levels of neuropeptide Y (NPY, an orexigenic neuropeptide) and proopiomelanocortin (POMC, a precursor of anorexigenic neuropeptides) in growing chickens at 7, 14, 21 and 28 days of age. Hypothalamic NPY mRNA levels were significantly (P<0.05) decreased after 14 days of age, whereas hypothalamic POMC mRNA levels were significantly (P<0.05) increased at 28 days of age. The percentage of abdominal fat was significantly increased after 14 days of age in chickens. We next examined the correlation of hypothalamic NPY and POMC mRNA levels and several parameters at 28 days of age. There were no significant correlations between hypothalamic mRNA levels of NPY or POMC and the percentage of abdominal fat. These findings suggest that the gene expressions of NPY and POMC do not depend on adiposity in chickens, at least in 28-day-old layer chickens.

  WILEY, May 2015, ANIMAL SCIENCE JOURNAL, 86(5) (5), 517 - 522, English

  [Refereed]

  Scientific journal


• Intracerebroventricular administration of chicken glucagon-like peptide-2 potently suppresses food intake in chicks

  Kazuhisa Honda, Takaoki Saneyasu, Tomohiko Shimatani, Koji Aoki, Takuya Yamaguchi, Kiwako Nakanishi, Hiroshi Kamisoyama

  Glucagon-related peptides, such as glucagon-like peptide (GLP)-1, GLP-2 and oxyntomodulin (OXM), are processed from an identical precursor proglucagon. In mammals, all of these peptides are suggested to be involved in the central regulation of food intake. We previously showed that intracerebroventricular administration of chicken OXM and GLP-1 significantly suppressed food intake in chicks. Here, we show that central administration of chicken GLP-2 potently suppresses food intake in chicks. Male 8-day-old chicks (Gallus gallus domesticus) were used in all experiments. Intracerebroventricular administration of chicken GLP-2 significantly suppressed food intake in chicks. Plasma glucose concentration was significantly decreased by chicken GLP-2, whereas plasma nonesterified fatty acid concentration was significantly increased. Intracerebroventricular administration of chicken GLP-2 did not affect plasma corticosterone concentration. In addition, the anorexigenic effect of GLP-2 was not reversed by the corticotropin-releasing factor (CRF) receptor antagonist -helical CRF, suggesting that CRF is not a downstream mediator of the anorexigenic pathway of GLP-2 in chicks. Intracerebroventricular administration of an equimolar amount of GLP-1 and GLP-2, but not OXM, significantly suppressed food intake in both broiler and layer chicks. All our findings suggest that GLP-2 functions as a potent anorexigenic peptide in the brain, as well as GLP-1, in chicks.

  WILEY, Mar. 2015, ANIMAL SCIENCE JOURNAL, 86(3) (3), 312 - 318, English

  [Refereed]

  Scientific journal


• Chicken heat shock protein HSPB1 increases and interacts with alpha B-crystallin in aged skeletal muscle

  Shuji Ueda, Yoshito Kokaji, Shunsaku Simizu, Kazuhisa Honda, Ken-ichi Yoshino, Hiroshi Kamisoyama, Yasuhito Shirai, Minoru Yamanoue

  International trading markets of meat require the animal's age information to prevent cross-contamination of ineligible meat products. Individual livestock age is either evaluated from physiological features or verified by breeding history. However, it remains impossible to perform age verification on meat when a suspicion of error occurred in the importing country. To investigate an age-related protein in skeletal muscle of livestock, we compared protein expression among chicken pectoralis major of different ages. Results indicated that the level of expression of chicken HSPB1, one of the small heat shock proteins, was increased in aged muscles. On the other hand, other heat shock proteins, heat shock factors, and myosin heavy chain isoform did not change the expression levels in aged chicken muscle. In addition, we identified that alpha B-crystallin interacted with HSPB1 in aged chicken muscle. These results suggest that HSPB1 protein forms complexes with alpha B-crystallin in aged chicken muscle and suppose to become the candidate of age-related bio-marker for verifying the age of chicken meat.

  TAYLOR & FRANCIS LTD, 2015, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 79(11) (11), 1867 - 1875, English

  [Refereed]

  Scientific journal


• Dietary mannanase-hydrolyzed copra meal improves growth and increases muscle weights in growing broiler chickens

  Masahisa Ibuki, Yu Yoshimoto, Mariko Inui, Kensuke Fukui, Hiroko Yonemoto, Takaoki Saneyasu, Kazuhisa Honda, Hiroshi Kamisoyama

  The utilization of copra meal as a feed ingredient is limited because it contains a high level of mannan. However, recent findings indicate that the effect of copra meal on growth performance in broiler chickens can be improved by the supplementation of mannanase in the diet. In the present study, we examined the effect of mannanase-hydrolyzed copra meal (MCM) on growth performance and muscle protein metabolism in growing broiler chickens (Gallus gallus domesticus). Forty 8-day-old male broiler chicks were assigned to two groups (four birds in each pen, five replicates) and fed either a commercial diet (as a control diet) or a diet containing MCM at 0.2% until 22 days of age. Dietary MCM significantly increased the weights of body, breast muscle, and thighs in chickens, whereas the weights of abdominal adipose tissue and liver were not affected. Cumulative feed intake was significantly increased by MCM. Dietary MCM significantly decreased plasma 3-methylhistidine level. The messenger RNA and protein levels of muscle protein metabolism-related factors were not altered by MCM. These findings suggest that the growth-promoting effect of MCM is related to the suppression of muscle proteolysis in growing broiler chickens.

  Corresponding, WILEY, May 2014, ANIMAL SCIENCE JOURNAL, 85(5) (5), 562 - 568, English

  [Refereed]

  Scientific journal


• Intracerebroventricular administration of novel glucagon-like peptide suppresses food intake in chicks

  Kazuhisa Honda, Takaoki Saneyasu, Takuya Yamaguchi, Tomohiko Shimatani, Koji Aoki, Kiwako Nakanishi, Hiroshi Kamisoyama

  Glucagon-related peptides such as glucagon, glucagon-like peptide-1, and oxyntomodulin suppress food intake in mammals and birds. Recently, novel glucagon-like peptide (GCGL) was identified from chicken brain, and a comparatively high mRNA expression level of GCGL was detected in the hypothalamus. A number of studies suggest that the hypothalamus plays a critical role in the regulation of food intake in mammals and birds. In the present study, we investigated whether GCGL is involved in the central regulation of food intake in chicks. Male 8-day-old chicks (Gallus gallus) were used in all experiments. Intracerebroventricular administration of GCGL in chicks significantly suppressed food intake. Plasma glucose level was significantly decreased by GCGL, whereas plasma corticosterone level was not affected. Central administration of a corticotrophin-releasing factor (CRF) receptor antagonist, alpha-helical CRF, attenuated GCGL-suppressed food intake. It seems likely that CRF receptor is involved in the GCGL-induced anorexigenic pathway. All our findings suggest that GCGL functions as an anorexigenic peptide in the central nervous system of chicks. (C) 2013 Elsevier Inc. All rights reserved.

  ELSEVIER SCIENCE INC, Feb. 2014, PEPTIDES, 52, 98 - 103, English

  [Refereed]

  Scientific journal


• Intracerebroventricular administration of chicken oxyntomodulin suppresses food intake and increases plasma glucose and corticosterone concentrations in chicks

  Kazuhisa Honda, Takaoki Saneyasu, Takuya Yamaguchi, Tomohiko Shimatani, Koji Aoki, Kiwako Nakanishi, Hiroshi Kamisoyama

  Elsevier Inc., Feb. 2014, Neuroscience Letters, 564(3) (3), 57 - 61, English

  [Refereed]

  Scientific journal


• Age-dependent changes in the mRNA levels of neuropeptide Y, proopiomelanocortin, and corticotropin-releasing factor in the hypothalamus in growing broiler chicks

  Takaoki Saneyasu, Kiwako Nakanishi, Hiroyuki Atsuta, Atsushi Ikura, Shin Hasegawa, Hiroshi Kamisoyama, Kazuhisa Honda

  Japan Poultry Science Association, Oct. 2013, Journal of Poultry Science, 50(4) (4), 364 - 369, English

  [Refereed]


• Enzymatically synthesized glycogen reduces lipid accumulation in diet-induced obese rats

  Takashi Furuyashiki, Rui Ogawa, Yoko Nakayama, Kazuhisa Honda, Hiroshi Kamisoyama, Nhiroki Takata, Michiko Yasuda, Takashi Kuriki, Hitoshi Ashida

  Based on a recent study indicating that enzymatically synthesized glycogen (ESG) possesses a dietary, fiber-like action, we hypothesized that ESG can reduce the risk of obesity. In this study, the antiobesity effects of ESG were investigated in a model of diet-induced obesity. Male Sprague-Dawley rats were divided into 4 groups and fed a normal or high-fat diet, with or without 20% ESG, for 4 weeks. Body weight, food intake, lipid deposition in the white adipose tissues and liver, fecal lipid excretion, and plasma lipid profiles were measured. At week 3, the body fat mass was measured using an x-ray computed tomography system, which showed that ESG significantly suppressed the high-fat diet induced lipid accumulation. Similar results were observed in the weight of the adipose tissue after the experiment. Moreover, ESG significantly suppressed the lipid accumulation in the liver but increased fecal lipid excretion. The plasma concentrations of triacylglycerol and nonesterified fatty acid were lowered after a high-fat diet, whereas the total bile acid concentration was increased by ESG. However, the hepatic messenger RNA (mRNA) levels of enzymes related to lipid metabolism were not affected by ESG. Conversely, the mRNA levels of long-chain acyl-CoA dehydrogenase and medium-chain acyl-CoA dehydrogenase were up-regulated by ESG in the muscle. These results suggest that the combined effects of increased fecal lipid excretion, increased mRNA levels of enzymes that oxidize fatty acids in the muscle, and increased total bile acid concentration in the plasma mediate the inhibitory effect of ESG on lipid accumulation. (C) 2013 Elsevier Inc. All rights reserved.

  PERGAMON-ELSEVIER SCIENCE LTD, Sep. 2013, NUTRITION RESEARCH, 33(9) (9), 743 - 752, English

  [Refereed]

  Scientific journal


• Effects of short-term refeeding on the expression of genes involved in lipid metabolism in chicks (Gallus gallus)

  Takaoki Saneyasu, Miho Shiragaki, Kiyotaka Kurachi, Hiroshi Kamisoyama, Kazuhisa Honda

  The aim of this study was to analyze the expression patterns of key genes involved in lipid metabolism in response to feeding in chicks. A total of 18 thirteen day-old male chicks were fasted for 12 h. The mRNA levels of the genes in the liver and white adipose tissue were analyzed after 0, 2, and 4 h of refeeding. The mRNA levels of sterol regulatory element-binding protein (SREBP) 1, liver X receptor alpha, peroxisome proliferator-activated receptor (PPAR) gamma, acetyl-CoA carboxylase alpha and fatty acid synthase were significantly increased after 2 h of refeeding. In contrast, the mRNA levels of PPAR alpha and carnitine palmitoyltransferase 1a were significantly decreased after 2 h of refeeding. The mRNA level of acyl-CoA oxidase was significantly decreased after 4 h of refeeding. The mRNA levels of cholesterol metabolism-related genes such as SREBP2 and 3-hydroxy-3-methylglutaryl-CoA reductase were significantly increased after 2 h of refeeding. In the white adipose tissue, the mRNA level of PPAR gamma was significantly increased after 2 h of refeeding, whereas the mRNA level of adipose triglyceride lipase was significantly decreased after 4 h of refeeding. These results demonstrated that expression of lipid metabolism-related genes is regulated by short-term refeeding in chicks. (C) 2013 Elsevier Inc. All rights reserved.

  ELSEVIER SCIENCE INC, Sep. 2013, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 166(1) (1), 1 - 6, English

  [Refereed]

  Scientific journal


• Effects of short term fasting on the expression of genes involved in lipid metabolism in chicks

  Takaoki Saneyasu, Miho Shiragaki, Kohei Nakanishi, Hiroshi Kamisoyama, Kazuhisa Honda

  The aim of this study was to analyze the expression patterns of key genes involved in lipid metabolism in response to short term fasting in chicks (Gallus gallus). The mRNA level of the genes was analyzed after 0, 2, and 4 h of fasting in the liver and white adipose tissue. In the liver, the mRNA level of peroxisome proliferator-activated receptor a was significantly increased after 2 h of fasting. The mRNA levels of carnitine palmitoyltransferase la and acyl-CoA oxidase were significantly increased after 4 h of fasting. In contrast, the mRNA levels of sterol regulatory element-binding protein 1, acetyl-CoA carboxylase alpha, and fatty acid synthase were significantly decreased after 4 h of fasting. The mRNA levels of cholesterol metabolism-related genes such as 3-hydroxy-3-methylglutaryl-CoA reductase and cholesterol 7 alpha-hydroxylase were significantly decreased after 4 h of fasting. In the white adipose tissue, the mRNA level of adipose triglyceride lipase was significantly increased after 4 h of fasting. In contrast, the mRNA levels of peroxisome proliferator-activated receptor gamma and lipoprotein lipase were significantly decreased after 4 h of fasting. These results demonstrated that the gene expression of lipid metabolism-related genes is regulated by short term fasting in both the liver and WAT in chicks. (C) 2013 Elsevier Inc. All rights reserved.

  ELSEVIER SCIENCE INC, Jun. 2013, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 165(2) (2), 114 - 118, English

  [Refereed]

  Scientific journal


• Effect of Dietary beta-1,4-Mannobiose on the Growth of Growing Broiler Chicks

  Masahisa Ibuki, Yu Yoshimoto, Hiroki Yamasaki, Kazuhisa Honda, Kensuke Fukui, Hiroko Yonemoto, Shin Hasegawa, Hiroshi Kamisoyama

  We investigated the effects of dietary beta-1,4-mannobiose (MNB) on the weights of body, breast muscles, thighs, liver, and abdominal adipose tissue in growing broiler chicks to evaluate whether MNB can be used as a feed additive for broiler chicks. A total of 24 eight day-old male broiler chicks were allocated to two cages and fed a control diet or a 0.01% MNB-supplemented diet for 14 days. Dietary MNB significantly increased the relative weight of breast muscles, whereas the weights of the body, thighs, liver and abdominal adipose tissue were not affected. The myostatin mRNA level in the breast muscle was significantly reduced by MNB. Since myostatin is a negative regulator of myoblast proliferation and differentiation, it is possible that the downregulation of myostatin gene expression is involved in the increased breast muscle growth with MNB. The plasma 3-methyl histidine level, which is known to be a nonmetabolizable amino acid marker of myofibrillar protein catabolism, and the breast muscle atrogin-1 mRNA level, which is involved in protein catabolism, were not affected by dietary MNB. In addition, MNB did not affect protease mRNA levels in the breast muscles. These results suggest that MNB does not affect proteolysis in the breast muscles. All our findings suggest that MNB is a promising candidate feed additive to improve the meat yield of broiler chickens.

  Corresponding, JAPAN POULTRY SCIENCE ASSOC, Apr. 2013, JOURNAL OF POULTRY SCIENCE, 50(2) (2), 120 - 125, English

  [Refereed]

  Scientific journal


• Effect of licorice flavonoid oil on cholesterol metabolism in high fat diet rats

  Kazuhisa Honda, Takaoki Saneyasu, Shin Hasegawa, Yuji Tominaga, Shinichi Yokota, Hiroshi Kamisoyama

  Dietary licorice fravonoid oil (LFO) significantly decreased hepatic cholesterol and plasma lipoprotein cholesterol levels in high-fat diet rats. It significantly suppressed hydroxymethylglutaryl-CoA synthase activity and increased cholesterol 7α-hydroxylase activity. The low density lipoprotein receptor mRNA level was significantly increased by LFO. These results suggest that dietary LFO improves cholesterol metabolism in obese animals.

  Japan Society for Bioscience, Biotechnology, and Agrochemistry, 2013, Bioscience, Biotechnology and Biochemistry, 77(6) (6), 1326 - 1328, English

  [Refereed]

  Scientific journal


• Dietary coenzyme Q10 suppressed hepatic hydroxymethylglutaryl-CoA reductase activity in laying hens

  Kazuhisa Honda, Takaoki Saneyasu, Tohru Motoki, Yuriko Park, Hiroshi Kamisoyama

  The effects of dietary coenzyme Q10 (CoQ10) on cholesterol metabolism in laying hens were investigated. Dietary CoQ10 significantly reduced egg yolk cholesterol content and suppressed hepatic hydroxymethylglutaryl-CoA reductase (HMGR) activity. It is therefore likely that CoQ10 acts as an HMGR inhibitor in the livers of laying hens, which in turn results in a reduction in egg-yolk cholesterol.

  Japan Society for Bioscience, Biotechnology, and Agrochemistry, 2013, Bioscience, Biotechnology and Biochemistry, 77(7) (7), 1572 - 1574, English

  [Refereed]

  Scientific journal


• The mechanism underlying the central glucagon-induced hyperglycemia and anorexia in chicks

  Kazuhisa Honda, Hiroshi Kamisoyama, Taku Uemura, Takashi Yanagi, Noboru Saito, Yohei Kurose, Kunio Sugahara, Kazuo Katoh, Shin Hasegawa

  We investigated the mechanism underlying central glucagon-induced hyperglycemia and anorexia in chicks. Male 8-day-old chicks (Gallus gallus) were used in all experiments. Intracerebroventricular administration of glucagon in chicks induced hyperglycemia and anorexia from 30 min after administration. However, the plasma insulin level did not increase until 90 mm after glucagon administration, suggesting that glucose-stimulated insulin secretion from pancreatic beta cells may be suppressed by central glucagon. The plasma corticosterone concentration significantly increased from 30 min to 120 min after administration, suggesting that central glucagon activates the hypothalamic pituitary adrenal (HPA) axis in chicks. However, central administration of corticotropin-releasing factor (CRF), which activates the HPA axis in chicken hypothalamus, significantly reduced not only food intake but also plasma glucose concentration, suggesting that CRF and the activation of the HPA axis are related to the glucagon-induced anorexia but not hyperglycemia in chicks. Phentolamine, an et-adrenergic receptor antagonist, significantly attenuated the glucagon-induced hyperglycemia, suggesting that glucagon induced hyperglycemia at least partly via a-adrenergic neural pathway. Co-administration of phentolamine and a-helical CRF, a CRF receptor antagonist, significantly attenuated glucagon-induced hyperglycemia and anorexia. It is therefore likely that central administration of glucagon suppresses food intake at least partly via CRF-induced anorexigenic pathway in chicks. (C) 2012 Elsevier Inc. All rights reserved.

  ELSEVIER SCIENCE INC, Nov. 2012, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 163(3-4) (3-4), 260 - 264, English

  [Refereed]

  Scientific journal


• Effect of Dietary Soybean-Germ Protein on Abdominal Fat Accumulation in Growing Broiler Chickens

  Tohru Motoki, Kazuhisa Honda, Sanshiro Saitoh, Hisashi Kataoka, Toshiro Sato, Hiroshi Kamisoyama, Shin Hasegawa

  In this study, we investigated the effects of dietary soybean-germ protein (SGP) on abdominal fat accumulation in growing broiler chickens. A total of 24 seven day-old male broiler chicks were allocated to two cages and fed a control diet or a SGP diet for 21 days. Dietary SGP significantly decreased the weight of abdominal adipose tissue. Hepatic triglyceride content and plasma levels of triglyceride and very low density lipoprotein-triglyceride were significantly decreased by dietary SGP. The enzymatic activity and mRNA level of fatty acid synthase in the liver were significantly decreased by dietary SGP. These results suggest that dietary SGP might reduce abdominal fat accumulation by downregulation of fatty acid synthesis in the liver of growing broiler chickens. The weights of breast muscles and thighs were significantly increased by dietary SGP. The ribosomal capacity of the breast muscles was significantly increased by dietary SGP. These results suggest that dietary SGP might increase skeletal muscle weight by upregulation of protein synthesis in the muscle of growing broiler chicken. All our findings suggest that SGP can be used as a new feed ingredient for broiler chickens.

  JAPAN POULTRY SCIENCE ASSOC, Oct. 2012, JOURNAL OF POULTRY SCIENCE, 49(4) (4), 282 - 290, English

  [Refereed]

  Scientific journal


• A comparative study of the central effects of melanocortin peptides on food intake in broiler and layer chicks

  Kazuhisa Honda, Takaoki Saneyasu, Shin Hasegawa, Hiroshi Kamisoyama

  Broiler chicks eat more food than layer chicks. However, the causes of the difference in food intake in the neonatal period between these strains are not clear. In this study, we examined the involvement of proopiomelanocortin (POMC)-derived melanocortin peptides alpha-, beta- and gamma-melanocyte-stimulating hormones (MSHs) in the difference in food intake between broiler and layer chicks. First, we compared the hypothalamic mRNA levels of POMC between these strains and found that there was no significant difference in these levels between broiler and layer chicks. Next, we examined the effects of central administration of MSHs on food intake in these strains. Central administration of alpha-MSH significantly suppressed food intake in both strains. Central administration of beta-MSH significantly suppressed food intake in layer chicks, but not in broiler chicks, while central administration of gamma-MSH did not influence food intake in either strain. It is therefore likely that the absence of the anorexigenic effect of beta-MSH might be related to the increased food intake in broiler chicks. (c) 2012 Elsevier Inc. All rights reserved.

  ELSEVIER SCIENCE INC, Sep. 2012, PEPTIDES, 37(1) (1), 13 - 17, English

  [Refereed]

  Scientific journal


• Effect of Dietary Potassium Diformate on the Growth of Growing Broiler Chickens

  Tohru Motoki, Hiroshi Kamisoyama, Kazuhisa Honda, Shin Hasegawa

  Potassium diformate (KDF), a chemical complex of formic acid and potassium formate, improves growth performance in pigs. The aim of the present study was to examine the effects of dietary KDF on the growth, nitrogen retention, intestinal pH, counts of Enterococcus faecalis, coliforms, and lactic acid bacteria in the cecum, and humoral immune response of growing broiler chickens. Twenty four male broiler chicks were randomly assigned into three groups (eight birds in each group). Each group was fed an antibiotics free commercial diet (as a control diet, 23% CP, 3,000 kcal of ME/kg), a control diet containing KDF at 1%, or a control diet containing antibiotics (50g titer/t Salinomycin, 50g titer/t Avilamycin) until 28 days of age. Dietary KDF significantly increased the weights of body, breast muscle, thighs and wings, whereas the weights of liver and abdominal fat were not affected. These findings suggest that the increase of body weight by dietary KDF might be due to the increased muscle weight. Dietary KDF did not affect nitrogen retention. Dietary KDF did not affect the intestinal pH, and the counts of Enterococcus faecalis. coliforms, and lactic acid bacteria in the cecum. Hemagglutination titer was not affected by dietary KDF. Thus, although the mechanisms of the growth promotion by dietary KDF are not clear, our findings suggest that the KDF might promote growth of broiler chickens at least in the early phase of growth.

  JAPAN POULTRY SCIENCE ASSOC, Oct. 2011, JOURNAL OF POULTRY SCIENCE, 48(4) (4), 247 - 253, English

  [Refereed]

  Scientific journal


• Neuropeptide Y effect on food intake in broiler and layer chicks

  Takaolci Saneyasu, Kazuhisa Honda, Hiroshi Kamisoyama, Atsushi Ikura, Yoko Nakayama, Shin Hasegawa

  Broiler chicks eat more food than layer chicks. In this study, we examined the involvement of orexigenic peptide neuropeptide Y (NPY) in the difference in food intake between broiler and layer chicks (Gallus gallus). First, we compared the hypothalamic mRNA levels of NPY and its receptors (Y1 and Y5 receptors) between these strains at 1, 2, 4, and 8 days of age. Daily food intake was significantly higher in broiler chicks than layer chicks after 2 days of age. However, the hypothalamic NPY mRNA level was significantly lower in broiler chicks than layer chicks except at 8 days of age. In addition, the mRNA levels of NPY receptors were also significantly lower in broiler chicks than layer chicks at 2 and 4 days of age (Y1 receptor) or 2 days of age (Y5 receptor). These results suggest that the differences in the expressions of hypothalamic NPY and its receptors do not cause the increase in food intake in broiler chicks. To compare the orexigenic effect of NPY between broiler and layer chicks, we next examined the effects of central administration of NPY on food intake in these strains. In both strains, central administration of NPY significantly increased food intake at 2,4 and 8 days of age. All our findings demonstrated that the increase in food intake in broiler chicks is not accompanied with the over-expression of NPY or its receptor. (C) 2011 Elsevier Inc. All rights reserved.

  ELSEVIER SCIENCE INC, Aug. 2011, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 159(4) (4), 422 - 426, English

  [Refereed]

  Scientific journal


• Alpha-melanocyte stimulating hormone plays an important role in the regulation of food intake by the central melanocortin system in chicks

  Takaoki Saneyasu, Kazuhisa Honda, Hiroshi Kamisoyama, Yoko Nakayama, Kengo Ikegami, Shin Hasegawa

  Proopiomelanocortin (POMC, a precursor of melanocortin peptides) neurons in the hypothalamus play an important role in the central regulation of food intake in mammals. There is evidence that human melanocortin peptides alpha-, beta- and gamma2-melanocyte-stimulating hormone (alpha-, beta- and gamma 2-MSH) significantly decreased food intake in chickens. However, the amino acid sequences of beta- and gamma 2-MSH of chickens are different from those of humans whereas the amino acid sequence of alpha-MSH is conserved between these species. In the present study, we examined the effects of the central administration of alpha-, chicken beta-, and chicken gamma 2-MSH on food intake in chicks. Central administration of alpha-MSH significantly suppressed food intake in chicks. In contrast, beta- and gamma 2-MSH did not influence food intake in chicks. Central administration of HS014, a melanocortin 4 receptor antagonist, significantly reversed the anorexigenic action of alpha-MSH, suggesting that this action is mediated by the melanocortin 4 receptor in chicks as well as in mammals. These results suggest that alpha-MSH may play an important role in the regulation of food intake by the central melanocortin system in chicks. (C) 2011 Elsevier Inc. All rights reserved.

  ELSEVIER SCIENCE INC, May 2011, PEPTIDES, 32(5) (5), 996 - 1000, English

  [Refereed]

  Scientific journal


• A Fibronectin-Independent Mechanism of Collagen Fibrillogenesis in Adult Liver Remodeling

  Kei Moriya, Eunnyung Bae, Kazuhisa Honda, Keiko Sakai, Takehisa Sakaguchi, Ikuko Tsujimoto, Hiroshi Kamisoyama, Douglas R. Keene, Takako Sasaki, Takao Sakai

  BACKGROUND & AIMS: Fibrosis is an abnormal extension of the wound healing process that follows tissue damage; it is involved in pathogenesis in a variety of chronic diseases. The formation of extracellular matrix is an essential response in wound healing. Although it has been proposed that collagen organization and assembly depend on the fibronectin matrix in culture, the contribution of fibronectin to these processes remains to be defined in vivo. METHODS: We generated a conditional, fibronectin-deficient mouse model of liver injury and explored whether fibronectin would be a suitable target for preventing extensive collagen deposits and scar formation that could lead to liver fibrosis. RESULTS: The lack of fibronectin did not interfere with reconstruction of collagen fibril organization in response to liver injury. Signaling by transforming growth factor-beta and type V collagen were required for collagen fibrillogenesis during remodeling of adult liver tissue. CONCLUSIONS: Transforming growth factor-beta and type V collagen are targets for regulating the initial fibrogenic response to liver damage.

  W B SAUNDERS CO-ELSEVIER INC, May 2011, GASTROENTEROLOGY, 140(5) (5), 1653 - 1663, English

  [Refereed]

  Scientific journal


• Amino Acid Digestibility of Rice at Different Sites of Chicken Intestines

  Kazuhisa Honda, Hiroshi Kamisoyama, Kengo Ikegami, Shin Hasegawa

  Global demand of corns for feed and fuel will be increasing at a rapid pace in the near future. Rice is one of the candidate cereals as a substitute of corn. However, the amino acid digestibility of rice in the gastrointestinal tract in chickens has not yet been investigated. The present study was conducted to investigate the amino acid digestibility of hulled rice using fistulized chickens. Chickens were fistulized to either the distal end of the jejunum, the middle part of the ileum, the distal end of the ileum or the distal end of the rectum. Intestinal digesta were collected from each site of the intestines, and the contents of amino acids were measured. Chickens were fed a rice diet, a rice-fish meal diet or a corn-based diet. There was no significant difference in the true digestibilities of all measured amino acids between the sites of chicken intestines. However, the true digestibilities of all measured amino acids without arginine of the rice diet were significantly higher than those of the corn-based diet. The true digestibilities of all measured amino acids without tyrosine of the rice-fish meal diet were significantly higher than those of the corn-based diet. These results suggest that rice might be used as a substitute of corn in chicken feed.

  Lead, JAPAN POULTRY SCIENCE ASSOC, Apr. 2011, JOURNAL OF POULTRY SCIENCE, 48(2) (2), 85 - 91, English

  [Refereed]

  Scientific journal


• Comparison of Amino Acid Digestibility of Dietary Cereals at Different Sites of Chicken Intestines

  Hiroshi Kamisoyama, Kazuhisa Honda, Shin Hasegawa

  The present study was conducted to investigate the effects of dietary cereals such as corn, sorghum, wheat, hulled barley and naked barley on amino acid digestibility using fistulized chickens. Chickens were fistulized to either the distal end of the jejunum, the middle part of the ileum, the distal end of the ileum or the distal end of the rectum. The cereals were supplemented as the sole source of protein in each experimental diet. Intestinal digesta were collected from each site of the intestines, and the contents of amino acids were measured. There was no significant difference in the true digestibilities of all measured amino acids between the sites of chicken intestines. However, the true digestibilities of 13 amino acids (Asp, Thr, Ser, Gly, Ala, Val, Met, Ile, Leu, Tyr, Phe, His and Arg) in the hulled barley group, 10 amino acids (Asp, Thr, Ser, Gly, Ala, Val, Met, Ile, Leu and Tyr) in the naked barley group, nine amino acids (Asp, Thr, Gly, Ala, Met, Ile, Leu, Tyr and Arg) in the wheat group and five amino acids (Gly, Met, Lys, His and Arg) in the sorghum group were lower than those in the corn group. These results clearly demonstrate that corn shows the highest amino acid digestibility among cereals used in this study and that there is no significant effect of sampling site on the amino acid digestibility of the dietary cereals.

  JAPAN POULTRY SCIENCE ASSOC, Jan. 2011, JOURNAL OF POULTRY SCIENCE, 48(1) (1), 19 - 24, English

  [Refereed]

  Scientific journal


• Effects of Dietary Protein Levels on Amino Acid Digestibility at Different Sites of Male Adult Chicken Intestines

  Hiroshi Kamisoyama, Kazuhisa Honda, Shinpei Kubo, Shin Hasegawa

  The present study was conducted to investigate the effects of varying dietary protein levels on amino acid digestibility at different sites of fistulized chicken intestines. Chickens were fistulized to either the middle part of the jejunum (MJ), the distal end of the jejunum, the middle part of the ileum, the distal end of the ileum or the distal end of the rectum (DR). Intestinal digesta were collected from each site of the intestine, and contents of amino acids were measured. The true digestibilities of all amino acids in intestinal digests at MJ were significantly lower than those at DR in the 10% protein group. In the 15% protein group, the true digestibilities of Asp, Thr, Glu, Pro, Gly, Val, Met and Ile in intestinal digesta at MJ were significantly lower than those at DR. The true digestibility of Ser in intestinal digesta at MJ was significantly lower than that at DR in the 20% protein group. In the 30% protein group, there was no significant difference in the true digestibilities of amino acids in intestinal digesta between the sites of the intestine. The true digestibilities of all amino acids in intestinal digests at MJ in the 10% protein group were significantly lower than those in other groups. These results clearly demonstrated that dietary protein levels influence amino acid digestibility at MJ in chickens.

  JAPAN POULTRY SCIENCE ASSOC, Jul. 2010, JOURNAL OF POULTRY SCIENCE, 47(3) (3), 220 - 226, English

  [Refereed]

  Scientific journal


• Effects of Dietary Fat Levels on Amino Acid Digestibility at Different Sites of Chicken Intestines

  Kazuhisa Honda, Hiroshi Kamisoyama, Shinpei Kubo, Tomoki Motoori, Shin Hasegawa

  The present study was conducted to investigate the effects of varying dietary fat levels (3%-10%) on amino acid digestibility at different sites of fistulized chicken intestines. Chickens were fistulized to either the middle part of the jejunum (MJ), the distal end of the jejunum (DJ), the middle part of the ileum, the distal end of the ileum or the distal end of the rectum. Intestinal digesta were collected from each site of the intestine, and contents of amino acids were measured. True digestibilities of individual amino acids were not affected by the sites of intestines in the 3, 5 and 8% fat groups. The true digestibility of total amino acid in intestinal digesta at the MJ was significantly lower than that at the DJ in the 10% fat group. In addition, true digestibilities of individual amino acids at the MJ tended to be lower than those at the DJ in the 10% fat group. Comparison of the data at the MJ between groups showed that the true digestibilities of total amino acid and Met in intestinal digesta in the 10% fat group were significantly lower than those in other groups. The true digestibilities of all amino acids, except for Met, in intestinal digesta at the MJ in the 10% fat group tended to be lower than those in other groups. These results suggest that dietary fat levels influence the digestion of amino acids at the proximal part of the jejunum in chickens.

  Lead, JAPAN POULTRY SCIENCE ASSOC, Jul. 2010, JOURNAL OF POULTRY SCIENCE, 47(3) (3), 227 - 235, English

  [Refereed]

  Scientific journal


• Transfer of Dietary Coenzyme Q10 into the Egg Yolk of Laying Hens

  Hiroshi Kamisoyama, Kazuhisa Honda, Kanako Kitaguchi, Shin Hasegawa

  Coenzyme Q10 (CoQ10), a potent lipophilic antioxidant, is a naturally occurring compound with a ubiquitous distribution in nature, and CoQ10 is used as a dietary supplement to combat aging. There is evidence that lipid soluble nutrients can be transferred into the egg yolk from the feed in laying hens. It is therefore possible that feeding CoQ10 to laying hens increases the content of CoQ10 in the egg yolk. In the present study, we investigated the effect of dietary CoQ 10 on its content in the egg yolk of laying hens. Twenty 30 weeks-old Boris Brown hens were assigned based on egg production rate and egg weight to 2 groups (10 birds in each group) and fed a diet containing CoQ10 at 0 or 0.8% for 28 days. Dietary CoQ10 did not affect average egg production rate, feed efficiency, egg weight, and egg yolk weight. CoQ10 content in the egg yolk was increased significantly at 7, 14, 21 and 28 days of the experimental period. Hepatic CoQ10 content and plasma very low density lipoprotein y (VLDLy) CoQ10 concentration in 0.8% group were increased significantly compared with those in 0% group. Dietary CoQ10 significantly decreased cholesterol concentration in the egg yolk at 21(st) and 28(th) day of the experimental period. These findings suggest that, in CoQ10-fed laying hens, the increase of hepatic CoQ10 elevates the plasma VLDLy CoQ10 concentration, which in turn results in a high CoQ10 content in the egg yolk of laying hens.

  JAPAN POULTRY SCIENCE ASSOC, Jan. 2010, JOURNAL OF POULTRY SCIENCE, 47(1) (1), 28 - 33, English

  [Refereed]

  Scientific journal


• Effect of Dietary Coenzyme Q10 on Cholesterol Metabolism in Growing Chickens

  Kazuhisa Honda, Hiroshi Kamisoyama, Tomoki Motoori, Takaoki Saneyasu, Shin Hasegawa

  Coenzyme Q10 (CoQ10), a potent lipophilic antioxidant, is a naturally occurring compound with a ubiquitous distribution in nature and is used as a dietary supplement to combat aging. In rats, there is evidence that coenzyme Q9, major coenzyme Q homologue in rodents, suppresses hepatic cholestcrogenesis and decreases plasma total cholesterol concentration. In this study, we investigated the effect of dietary CoQ10 on cholesterol metabolism in growing chickens. The supplementation of CoQ10 in a diet significantly decreased the total cholesterol levels in the liver and plasma. Plasma very low density lipoprotein (VLDL) cholesterol concentration was significantly decreased by dietary CoQ10. The enzymatic activity of hepatic hydroxymethylglutaryl-coenzyme A reductase (HMGR), the rate-limiting enzyme of the cholesterol synthetic pathway, was significantly decreased by dietary CoQ10, whereas the mRNA level of HMGR was not affected. These findings suggest that dietary CoQ10 suppresses hepatic cholesterogenesis by the inhibition of HMGR activity at the posttranscriptional level in chickens, which in turn decreases plasma VLDL cholesterol concentration.

  Lead, JAPAN POULTRY SCIENCE ASSOC, Jan. 2010, JOURNAL OF POULTRY SCIENCE, 47(1) (1), 41 - 47, English

  [Refereed]

  Scientific journal


• Effects of dietary protein levels on the nutrient digestibility at different sites of chicken intestines

  Hiroshi Kamisoyama, Kazuhisa Honda, Yutaka Isshiki, Shin Hasegawa

  JAPAN POULTRY SCIENCE ASSOC, Oct. 2009, The Journal of Poultry Science, 46(3) (3), 193 - 197, English

  [Refereed]

  Scientific journal


• Effects of Dietary Fat Levels on Nutrient Digestibility at Different Sites of Chicken Intestines

  Kazuhisa Honda, Hiroshi Kamisoyama, Yutaka Isshiki, Shin Hasegawa

  The present study was conducted to investigate the effects of varying dietary fat levels (3%-10%) on the digestibilities of crude protein, crude fat (CF), nitrogen-free extract and ash at different sites of fistulized chicken intestines. Chickens were fistulized to either the middle part of the jejunum (MJ), the distal end of the jejunum (DJ), the middle part of the ileum, the distal end of the ileum or the distal end of the rectum. Intestinal digesta were collected from each site of intestine, and contents of crude protein, crude fat, nitrogen-free extract and ash were measured. The true digestibility of crude protein in intestinal digesta at MJ in the 10% CF group was significantly lower than that in other groups. The digestibility of crude fat in intestinal digesta at MJ in the 10% CF group was also significantly lower than that in other groups. The digestibility of nitrogen-free extract at MJ and DJ in the 10% CF group was significantly lower than that in other groups. There were no significant differences in digestibility of ash among groups. These results clearly demonstrate that dietary fat levels influence the digestibility of protein, fat, and carbohydrate at MJ in chicken.

  Lead, JAPAN POULTRY SCIENCE ASSOC, Oct. 2009, JOURNAL OF POULTRY SCIENCE, 46(4) (4), 291 - 295, English

  [Refereed]

  Scientific journal


• Corticotropin-releasing factor is a downstream mediator of the beta-melanocyte-stimulating hormone-induced anorexigenic pathway in chicks

  Hiroshi Kamisoyama, Kazuhisa Honda, Takaoki Saneyasu, Kunio Sugahara, Shin Hasegawa

  Proopiomelanocortin (POMC, a precursor of anorexigenic neuropeptides) neurons in hypothalamus suppresses food intake in both mammals and chickens. In mammals, several lines of evidence suggest that POMC-derived anorexigenic peptides upregulate mRNA levels of anorexigenic peptides such as corticotropin-releasing factor (CRF) and thyrotropin-releasing factor and downregulate mRNA levels of orexigenic peptides such as orexin and melanin-concentrating hormone. However, the POMC-induced anorexigenic pathway in chickens has not been well characterized. In the present study, we investigated how POMC neurons regulate mechanisms of food intake using an anorexigenic peptide, beta-melanocyte-stimulating hormone (beta-MSH), derived from the post-transcriptional cleavage of POMC. Central administration of beta-MSH in chicks significantly suppressed food intake, and importantly, this suppression was accompanied by a significant upregulation of CRF mRNA levels. Furthermore, the CRF type 2 receptor antagonist alpha-helical CRF significantly reversed the anorexigenic action of beta-MSH. These findings indicate that CRF and its receptor, CRF type 2 receptor, act as the major mediators in beta-MSH-induced anorexigenic action in chicks. beta-MSH significantly increased orexin mRNA levels and did not alter mRNA levels of thyrotropin-releasing factor and melanin-concentrating hormone in chicks, suggesting that the beta-MSH-induced anorexigenic pathway in chicks is different from that in mammals. Increases in orexin mRNA levels were accompanied by significant decreases in plasma glucose concentration, suggesting that orexin mRNA might be stimulated by beta-MSH-induced hypoglycemia. Thus, this study demonstrates the direct evidence that CRF is a critical downstream target in the beta-MSH-induced anorexigenic pathway in chicks. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

  ELSEVIER IRELAND LTD, Jul. 2009, NEUROSCIENCE LETTERS, 458(3) (3), 102 - 105, English

  [Refereed]

  Scientific journal


• The molecular mechanism underlying the reduction in abdominal fat accumulation by licorice flavonoid oil in high fat diet-induced obese rats

  Kazuhisa Honda, Hiroshi Kamisoyama, Yuji Tominaga, Shinichi Yokota, Shin Hasegawa

  Licorice (Glycyrrhiza glabra) has been widely used in traditional medicines, and its flavonoid oil (LFO) decreases abdominal adipose tissue weight in mammals. In the present study, we investigated the molecular mechanisms underlying the decrease in abdominal adipose tissue weight by LFO. LFO significantly decreased the mRNA levels of rate-limiting enzymes in the hepatic fatty acid synthetic pathway, whereas LFO significantly increased the mRNA levels of a rate-limiting enzyme in the hepatic fatty acid oxidative pathway. LFO significantly decreased the mRNA levels of sterol regulatory element-binding protein-1c (SREBP-1c) (a transcription factor that promotes hepatic fatty acid synthesis), whereas the mRNA levels of peroxisome proliferator-activated receptor-alpha (PPAR-alpha) (a transcription factor that promotes hepatic fatty acid oxidation) was significantly increased. All our findings suggest that the decrease in abdominal adipose tissue weight by LFO is mediated by the transcriptional regulation of SREBP-1c and PPAR-alpha in the liver. Thus, we infer that the natural ingredient LFO is a promising candidate for use as a feed additive to reduce abdominal fat accumulation in domestic animals.

  Lead, WILEY, 2009, ANIMAL SCIENCE JOURNAL, 80(5) (5), 562 - 569, English

  [Refereed]

  Scientific journal


• Effects of central administration of glucagon on feed intake and endocrine responses in sheep

  Yohei Kurose, Hiroshi Kamisoyama, Kazuhisa Honda, Yoshiyuki Azuma, Kunio Sugahara, Shin Hasegawa, Shigeki Kobayashi

  This study was conducted to investigate effects of glucagon intracerebroventricularly administered on feed intake and endocrine changes in sheep. Four male sheep (48-55 kg BW) were used. The animals were acclimatized to be fed alfalfa hay cubes at 12.00 hour. Human glucagon (40 and 80 mu g/0.5 mL) was injected into the lateral ventricle at 12.00 hour. Blood samples were taken every 10 min from 30 min before to 180 min after the glucagon injection. Soon after the injection, the animals were given alfalfa hay cubes, and the amounts of the feed eaten within 2 h were measured. Feed intakes were significantly (P < 0.05) suppressed by 80 mu g of glucagon. Plasma glucose levels in control animals were gradually decreased after the feeding, whilst those in glucagon-treated animals were temporarily elevated just after the feeding and then kept higher than control levels. Plasma insulin was abruptly elevated after the feeding and was maintained at higher levels than before the feeding in all treatments. Plasma NEFA concentrations were decreased after the feeding in all treatments. A tendency of increase in plasma cortisol levels occurred in glucagon-injected animals. The present study provides the first evidence that glucagon directly acts on the brain, then inhibiting feeding behavior and inducing endocrine responses in ruminants.

  WILEY-BLACKWELL PUBLISHING, INC, 2009, ANIMAL SCIENCE JOURNAL, 80(6) (6), 686 - 690, English

  [Refereed]

  Scientific journal


• Investigation of the Anti-Obesity Action of Licorice Flavonoid Oil in Diet-Induced Obese Rats

  Hiroshi Kamisoyama, Kazuhisa Honda, Yuji Tominaga, Shinichi Yokota, Shin Hasegawa

  Licorice flavonoid oil (LFO), which contains hydrophobic flavonoids from Glycyrrhiza glabra LINNE, is a new ingredient for functional foods. In this study, we investigated the anti-obesity action of LFO in diet-induced obese rats. The addition of 2% LFO in a high-fat diet significantly decreased the weight of abdominal adipose tissue and the levels of hepatic and plasma triglycerides. We found that the enzymatic activities of acetyl-CoA carboxylase and fatty acid synthase, the rate-limiting enzymes in the fatty acid synthetic pathway, were significantly decreased by LFO, whereas the enzymatic activity of acyl-CoA dehydrogenase, the rate-limiting enzyme in the fatty acid oxidative pathway, was significantly increased. All our findings suggest that the anti-obesity action of LFO is controlled by regulation of the rate-limiting enzymes in the fatty acid synthetic and oxidative pathways in the liver.

  TAYLOR & FRANCIS LTD, Dec. 2008, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 72(12) (12), 3225 - 3231, English

  [Refereed]

  Scientific journal


• Epidermal hyperplasia and papillomatosis in mice with a keratinocyte-restricted deletion of csk

  Kazuhisa Honda, Takehisa Sakaguchi, Keiko Sakai, Christian Schmedt, Angel Ramirez, Jose Luis Jorcano, Alexander Tarakhovsky, Hiroshi Kamisoyama, Takao Sakai

  The Src family kinases (SFKs) are believed to play critical roles in malignant transformation, as well as in growth, invasion and dissemination of neoplastic tissue. Inhibition of SFK-mediated signal transduction and activation of downstream targets inhibits tumor progression. To determine whether constitutive activity of SFK per se is sufficient to induce tumorigenesis in vivo, we have generated a mouse model with a keratinocyte-restricted deletion of the SFK-negative regulator csk (Csk-K5 mice). Even though expression levels of SFKs were lower in C-terminal Src kinase (Csk)-null keratinocytes, activity levels were higher than in control keratinocytes. At the age of 3 months, all Csk-K5 mice displayed signs of chronic inflammation in dermis and epidermal hyperplasia. About 19% of Csk-K5 mice (7 out of 36) developed papillomatous lesions. However, these lesions did not show any signs of neoplastic transformation over the next 8 months. Epidermal hyperplasia and hyperkeratosis in Csk-K5 mice were associated with an increased number of stem cells in the interfollicular epidermis, an increased proliferation of basal keratinocytes and a delayed terminal differentiation of the suprabasal keratinocytes. Our results clearly demonstrate that even though SFK-mediated signaling promotes tumor progression, elevated activity of SFKs in vivo alone is not sufficient to induce neoplastic transformation.

  Lead, OXFORD UNIV PRESS, Oct. 2007, CARCINOGENESIS, 28(10) (10), 2074 - 2081, English

  [Refereed]

  Scientific journal


• Central administration of insulin suppresses food intake in chicks

  Kazuhisa Honda, Hiroshi Karnisoyama, Takaoki Saneyasu, Kunio Sugahara, Shin Hasegawa

  Although the orexigenic action of peptide hormones such as ghrelin and growth hormone releasing peptide is different between chickens and mammals, the anorexigenic action of peptide hormones is similar in both species. For example, central administration of peptide hormones such as leptin, cholecystokinin or glucagon has been shown to suppress food intake behavior in chickens and mammals. Central administration of insulin Suppresses food intake in mammals. However, the anorexigenic action of insulin in chickens has not yet been identified. In the present study, we investigated the effects of central administration of insulin on food intake in chicks. Intracerebroventricular administration of insulin in chicks significantly suppressed food intake. Central administration of insulin significantly upregulated mRNA levels of proopiomelanocortin (POMC), cocaine- and amphetamine-regulated transcript (CART) and corticotropin-releasing factor (CRF), but did not influence mRNA levels of neuropeptide Y and agouti-related protein in the hypothalamus. These results suggest that alpha-melanocyte stimulating hormone (alpha-MSH, an anorexigenic peptide from the post-translational cleavage of POMC), CART and CRF are involved in the anorexigenic action of insulin in chicks. Furthermore, central administration of alpha-MSH or CART significantly suppressed food intake. In addition, alpha-MSH significantly upregulated CRF rnRNA expression, suggesting that the anorexigenic action of alpha-MSH is mediated by CRF. Our findings demonstrate that insulin functions in chicks as an appetite-suppressive peptide in the central nervous system and suggest that this anorexigenic action is mediated by CART, alpha-MSH and CRF. (c) 2007 Elsevier Ireland Ltd. All rights reserved.

  Lead, ELSEVIER IRELAND LTD, Aug. 2007, NEUROSCIENCE LETTERS, 423(2) (2), 153 - 157, English

  [Refereed]

  Scientific journal


• Central administration of neuromedin U suppresses food intake in chicks

  Hiroshi Kamisoyama, Kazuhisa Honda, Takaoki Saneyasu, Kunio Sugahara, Shin Hasegawa

  The appetite-suppressive action of brain-gut peptides is similar in both chickens and mammals. In mammals, the brain-gut peptide neuromedin U (NMU) suppresses food intake via hypothalamic neuropeptides, corticotropin-releasing factor (CRF), oxytocin, and arginine-vasopressin. In chickens, central administration of CRF, oxytocin, or arginine-vasotocin (AVT, a nonmammalian equivalent of arginine-vasopressin) suppresses food intake. However. the anorexigenic action of NMU in chickens has not yet been identified. In the present study, we analyzed the effects of the central administration of NMU on food intake and hypothalamic mRNA levels of CRF, AVT and mesotocin (a nonmammalian equivalent of oxytocin) in chicks. Intracerebroventricular administration of NMU in chicks significantly suppressed food intake and induced wing-flapping behavior. NMU also significantly upregulated mRNA expression of CRF and AVT, but did not influence mRNA expression of mesotocin in the hypothalamus. These results suggest that NMU functions as an appetite-suppressive peptide via CRF and AVT in the central nervous system in chicks. (c) 2007 Elsevier Ireland Ltd. All rights reserved.

  ELSEVIER IRELAND LTD, Jun. 2007, NEUROSCIENCE LETTERS, 420(1) (1), 1 - 5, English

  [Refereed]

  Scientific journal


• Central administration of glucagon suppresses food intake in chicks

  Kazuhisa Honda, Hiroshi Kamisoyama, Noboru Saito, Yohei Kurose, Kunio Sugahara, Shin Hasegawa

  Food intake in chickens is regulated in a manner similar to that in mammals. Corticotropin-releasing factor (CRF), which increases the plasma corticosterone concentration, plays an important role as a mediator of many appetite-suppressive peptides in the central nervous system in both species. Central administration of glucagon suppresses food intake in rats. However, the anorexigenic action of glucagon in chicks has not yet been identified. In the present study, we investigated the effects of central administration of glucagon on food intake in chicks. Intracerebroventricular administration of glucagon in chicks significantly suppressed food intake and significantly induced hyperglycemia. In contrast, peripheral administration of the same dose of glucagon did not influence food intake and plasma glucose concentration. These results suggest that glucagon functions in chicks as an appetite-suppressive peptide in the central nervous system. Intracerebroventricular administration of glucagon in chicks also significantly increased CRF mRNA expression and plasma corticosterone concentration, suggesting that CRF acts as a downstream molecule for a glucagon-induced appetite-suppressive pathway in chicks. It is likely that the induction of hyperglycemia by central administration of glucagon is involved in its anorexigenic action, because peripheral administration of glucose in chicks suppressed food intake. These results suggest that CRF- and/or hyperglycemia-mediated pathways are involved in the anorexigenic action of glucagon in chicks. (c) 2007 Elsevier Ireland Ltd. All rights reserved.

  Lead, ELSEVIER IRELAND LTD, Apr. 2007, NEUROSCIENCE LETTERS, 416(2) (2), 198 - 201, English

  [Refereed]

  Scientific journal


• In vitro chromosomal aberration assay for globin digest

  Naohiko Higashikuni, Shizuyo Stou, Shigeaki Watanabe, Kazuhisa Honda, Masahiro Numata, Kyoichi Kagawa

  Nov. 2006, Japanese Pharmacology and Therapeitics, 34(11) (11), 1179 - 1183, Japanese

  [Refereed]

  Scientific journal


• Bacterial reverse mutation assay for globin digest

  Naohiko Higashikuni, Shizuyo Stou, Shigeaki Watanabe, Kazuhisa Honda, Masahiro Numata, Kyoichi Kagawa

  Nov. 2006, Japanese Pharmacology and Therapeitics, 34(11) (11), 1173 - 1178, Japanese

  [Refereed]

  Scientific journal


• In vivo mouse micronucleus assay for globin digest

  Naohiko Higashikuni, Shizuyo Stou, Shigeaki Watanabe, Kazuhisa Honda, Masahiro Numata, Kyoichi Kagawa

  Nov. 2006, Japanese Pharmacology and Therapeitics, 34(11) (11), 1185 - 1187, Japanese

  [Refereed]

  Scientific journal


• Isolation of cDNA encoding carboxypeptidase A from chicken pancreas

  Honda K, Kamisoyama H, Yagi K, Motoki T, Hasegawa S

  Lead, Nov. 2000, Animal Science Journal, 71(6) (6), 634 - 636, English

  [Refereed]

  Scientific journal


• Comparative effect of isolated soybean protein and casein on body fat deposition in chicks

  Honda K, Kamisoyama H, Kato H, Furuya N, Ishiwata H, Motoki T, Saito N, Hasegawa S

  The present study was conducted to evaluate the comparative effect of soybean protein and casein on body fat deposition in chicks. Chicks were fed an 18% protein diet (soybean protein or casein), and the effects of dietary protein type on growth performance, the lipid contents of the plasma and liver, and the concentrations of plasma glucose and corticosterone were examined. There was no differences in final body weight, and the concentrations of plasma glucose and free fatty acids between the two dietary groups. Hepatic triacylglycerol content and plasma triacylglycerol level, however, were significantly lower in the soybean protein-fed than in the casein-fed chicks. The abdominal fat weight and the plasma corticosterone concentration were markedly lower in the soybean protein-fed chicks. The results suggest that soybean protein in the diet has the effect of lowering the body fat deposition as compared with casein.

  Lead, Japan Poultry Science Association, Nov. 2000, Japanese Poultry Science, 37(6) (6), 366 - 373, English

  [Refereed]

  Scientific journal


• Purification and characterization of carboxypeptidase B from chicken pancreas

  Honda K, Kamisoyama H, Yagi K, Atsuji K, Motoki T, Ishiwata H, Hasegawa S

  Carboxypeptidase B was purified by about 74-fold with an overall recovery of 3.3% from chicken pancreas. The enzyme occurs as a monomer and has a molecular weight of about 35, 000 as estimated by polyacrylamide-gel electrophoresis (PAGE) and sodium dodecyl sulphate (SDS)-PAGE. The enzyme was stable below 55°C, and the optimal temperature was approximately 45°C. The activity of the enzyme was stable in the range of pH7.0-9.5, and was optimal at pH8.5. The Michaelis constant (Km) value for tuppuryl-L-arginine was estimated to be 0.07mM. The isoelectric point of the enzyme was at pH4.6. Although the activity of the enzyme was not inhibited by cobalt chloride, soybean trypsin inhibitor or phenylmethanesulfonyl fluoride, it was inhibited by o-phenanthroline, ethylenediaminetetraacetic acid, 8-hydroxyquinoline and cadmium acetate.

  Lead, Japanese Society of Animal Science, Sep. 2000, Animal Science Journal, 71(5) (5), 524 - 527, English

  [Refereed]

  Scientific journal


• Purification and characterization of carboxypeptidase A from chicken pancreas

  Honda K, Kamisoyama H, Yagi K, Motoki T, Ishiwata H, Hasegawa S

  Lead, Sep. 2000, Animal Science Journal, 71(5) (5), 520 - 523, English

  [Refereed]

  Scientific journal


• Production of recombinant chicken glucagon using E.Coli.

  Hiroshi Kamisoyama, Ken Yagi, Kazuhisa Honda, Norihisa Fujii, Tohru Motoki, Norihiko Furuya, Hiroko Ishiwata, Mikio Tamaki, Shin Hasegawa

  Recombinant chicken glucagon was successfully produced with a high level of expression in E. coli as a fusion protein with maltose-binding protein (MBP). The synthetic gene was designed to release glucagon, which does not contain glutamic acid residues, from MBP-glucagon fusion protein with BLase that specifically cleaves the peptide bond on the carboxyl side of the glutamic acid residue. The resulting glucagon was purified to homogeneity by a combination of reverse-phase HPLC and ion-exchange HPLC, and its structure was confirmed by amino acid composition analysis. The overall yield of the recombinant glucagon was approximately 3.9mg from 1l of culture. According to its ability to stimulate lipolysis in chicken adipocytes, it was shown that recombinant chicken glucagon possesses biological activity.

  Japanese Society of Animal Science, Jul. 2000, Animal Science Journal, 71(4) (4), 428 - 431, English

  [Refereed]

  Scientific journal


• Influences of dietary protein types on the suppressive food intake induced by intracerebroventricular administration of glucagon-like peptide-1 in chicks

  Honda K, Suzuki M, Kamisoyama H, Motoki T, Kano K, Yagi K, Sugahara K, Furuse M, Hasegawa S

  Lead, Jul. 2000, Japanese Poultry Science, 37(4) (4), 251 - 257, English

  [Refereed]

  Scientific journal


• Effects of dietary de-fatted beef and its fractions on plasma and hepatic triglyceride contents in chicks

  Honda K, Suzuki M, Kamisoyama H, Kato H, Iba S, Motoki T, Kano K, Yagi K, Sugahara K, Hasegawa S

  Lead, Jul. 2000, Japanese Poultry Science, 37(4) (4), 245 - 250, English

  [Refereed]

  Scientific journal


• Utilization of animal by-products as food materials by enzymatic treatment

  Kazuhisa Honda, Miki Suzuki, Masahiro Numata, Toyoo Nakamura

  For efficient utilization of animal by-products as food materials, we studied the processing of beef trimmings, liver, red blood cells (RBC) and cartilage by enzymatic treatment. As a result, the sufficient amount (53.9-75.0%) of protein which was the main nutritious component contained in the above organs was solubilized as a peptide form (M. W.<3, 000-6, 000) by the enzymatic treatment. It was considered that the nutrients of these organs were efficiently processed to create an easily absorbable protein source. The taste of cartilage extract (CE) was palatable, but that of RBC extract (RBCE) was not. It was considered that the better taste of CE was due to its characteristic amino acid composition, while the bitter taste of RBCE was due to the high content of bitter amino acids. Therefore, it is difficult to use enzymatic treatment alone for processing of RBC. The physiologically functional component (PFC; carnitine, heme iron, collagen, condroitin sulfate, calcium) were also extracted and concentrated by the enzymatic treatment. However, considering the taste of these extract, it is necessary to further purify PFC and/or improve the taste. Beef extract (BE) had the highest iron solubilizing ability among these extracts. It was supposed that the effect of BE was influenced by the amino acid composition.

  Lead, Japanese Society of Animal Science, Apr. 1998, Animal Science and Technolgy, 69(4) (4), 392 - 399, Japanese

  [Refereed]

  Scientific journal


• Effect of fasting on the adipose tissue weight in chicks, with reference to changes in triglyceride synthesis and fatty acid oxidation

  Shin Hasegawa, Tomohiro Kawakami, Kazuhisa Honda, Yuzo Hikami

  Triglyceride synthesis and fatty acid oxidation in the chick adipose tissue were studied by measuring the incorporation of [1-¹⁴C] acetate or [1-¹⁴C] palmitate into triglycerides and CO₂ in vivo and in vitro in order to investigate the effect of fasting on the adipose tissue weight in chicks. Fasting decreased markedly the incorporation of ¹⁴C-acetate into fatty acids and of ¹⁴C-palmitate into triglycerides. This result suggests that de novo triglyceride synthesis is reduced by fasting. Incorporation of ¹⁴C-palmitate into CO₂ was increased by fasting, whereas that of ¹⁴C-acetate into CO₂ was decreased. This result suggests that fatty acid oxidation is stimulated by fasting, and implies that β-oxidation of fatty acid may be increased by fasting.

  Japanese Society of Animal Science, Jun. 1994, Animal Science and Technology, 65(6) (6), 525 - 531, English

  [Refereed]

  Scientific journal


• Effect of fasting on the adipose tissue weight in chicks, with reference to changes in chemical composition and lipase activity

  Shin Hasegawa, Tomohiro Kawakami, Kazuhisa Honda, Yuzo Hikami

  Jun. 1994, Animal Science and Technology, 65(2) (2), 89 - 98, English

  [Refereed]

  Scientific journal


• Effect of fasting on the activities of lipogenic enzymes in chick adipose tissue

  Shin Hasegawa, Tomohiro Kawakami, Kazuhisa Honda, Yuzo Hikami

  Jun. 1994, Animal Science and Technology, 65(7) (7), 656 - 660, English

  [Refereed]


• Isolation of a cDNA encoding chicken insulin precursor

  Shin Hasegawa, Kazuhisa Honda, Koji Nata, Hideto Yonekura, Hiroshi Okamoto, Yuzo Hikami

  Japanese Society of Animal Science, Sep. 1991, Animal Science and Technology, 62(9) (9), 867 - 869, English

  [Refereed]

• World's Poultry Science Journal 書評 －2023年79巻3～4号－

  本田 和久

  Lead, 25 Apr. 2024, 日本家禽学会誌, 61(J1) (J1), J19 - J22, Japanese

  Book review


• World's Poultry Science Journal 書評 －2023年79巻1～2号－

  本田 和久

  Lead, 25 Oct. 2023, 日本家禽学会誌, 60(J2) (J2), J82 - J85, Japanese

  Book review


• World's Poultry Science Journal 書評 －2022年78巻3～4号－

  本田 和久

  Lead, 25 Apr. 2023, 日本家禽学会誌, 60(J1) (J1), J31 - J34, Japanese

  Book review


• 米作を基盤とした持続可能なわが国独自の鶏肉生産への挑戦

  本田 和久

  Lead, 24 Mar. 2023, 畜産の情報, (4) (4), 58 - 66, Japanese

  Introduction other


• World's Poultry Science Journal 書評 －2022年78巻1～2号－

  本田 和久

  Lead, Oct. 2022, 日本家禽学会誌, 59(J2) (J2), J35 - J38, Japanese

  Book review


• 飼育方法が鶏肉の肉質に及ぼす影響について

  本田 和久

  Lead, Sep. 2022, 鶏の研究, 97(10) (10), 34 - 37, Japanese

  [Invited]

  Introduction commerce magazine


• 鶏肉のおいしさの指標について

  本田 和久

  Lead, Jul. 2022, 鶏の研究, 97(8) (8), 17 - 21, Japanese

  [Invited]

  Introduction commerce magazine


• 鶏肉の美味しさの解析と高付加価値鶏肉生産への取組み

  本田 和久

  Lead, Jun. 2021, 鶏の研究, 6, 17 - 19, Japanese

  [Invited]

  Introduction commerce magazine


• World's Poultry Science Journal 書評 －2022年77巻3～4号－

  本田 和久

  Lead, Apr. 2021, 日本家禽学会誌, 59(J1) (J1), J6 - J9, Japanese

  Book review


• 食料自給率の向上および持続的社会の構築に向けた国産飼料米による国産鶏肉生産の検討

  本田 和久

  Lead, Nov. 2019, 畜産の情報, (11) (11), 55 - 62, Japanese

  Introduction other


• 国産飼料の増産・鶏肉の輸出促進に向けた飼料用米による国産鶏肉の美味しさの向上

  本田 和久

  Lead, Mar. 2019, 畜産の情報, (3) (3), 59 - 66, Japanese

  Introduction other


• 飼料米の給与が鶏肉の品質に及ぼす影響

  本田和久

  Oct. 2018, 畜産技術, (10) (10), 20 - 23

  [Invited]


• ブロイラーにおける飼育環境制御：照明色がブロイラー生産に及ぼす影響

  本田和久

  Jul. 2018, アグリバイオ, 2(7) (7), 19 - 23

  [Invited]


• Search and Evaluation of the Slow-type Muscle-increasing Factors Derived from Soybean

  實安 隆興, 進藤 悠, 本田 和久, 上曽山 博

  不二たん白質研究振興財団, Jun. 2017, 大豆たん白質研究, 19(37) (37), 147 - 152, Japanese


• ニワトリにおける食欲の調節

  本田和久

  畜産技術協会, May 2016, 畜産技術, (5) (5), 3 - 7, Japanese

  [Invited]


• 豚由来機能性食品素材に関する研究の最新動向

  HONDA KAZUHISA, SANEYASU TAKAOKI, KAMISOYAMA HIROSHI

  日本畜産振興会, Jun. 2014, 養豚の友, 6(543) (543), 43 - 45, Japanese

  [Invited]

  Introduction commerce magazine


• Biochemical studies on the digestive enzymes of pancreas in chicks

  HASEGAWA Shin, HONDA Kazuhisa, KAMISOYAMA Hiroshi

  家畜栄養生理研究会, 01 Apr. 2001, Proceedings of Japanese Society for Animal Nutrition and Metabolism, 45(1) (1), 51 - 64, Japanese


• 鶏の血漿及び肝臓トリグリセリド量に及ぼす脱脂牛肉並びにその画分給与の影響

  本田 和久, 鈴木 美紀, 上曽山 博, ほか7名

  日本万国家禽学会, Jul. 2000, 日本家禽学会誌, 37(4) (4), 245 - 250, Japanese


• 鶏におけるグルカゴン様ペプチド-1の脳内投与による摂食抑制効果に及ぼす食餌性タンパク質の影響

  本田 和久, 鈴木 美紀, 上曽山 博, ほか6名

  日本万国家禽学会, Jul. 2000, 日本家禽学会誌, 37(4) (4), 251 - 257, Japanese


• New trecd of biologically active substances I. Biologically active substances derived from of animal products and their effect.

  WAKAMATSU JUN'ICHI, HONDA KAZUHISA

  日本食品出版, Jan. 2000, ジャパンフードサイエンス, 39(1) (1), 52-59 - 59, Japanese

  Introduction commerce magazine


• ヘム鉄および牛骨粉のミネラル欠乏状態回復効果について

  本田 和久, 鈴木 美紀, 沼田 正寛, 中村 豊郎, 長尾 哲二

  30 Jun. 1999, 食肉の科学, 40(1) (1), 180 - 184, Japanese


• Utilization of Animal By-Products as Food Materials by Enzymatic Treatment

  HONDA Kazuhisa, SUZUKI Miki, NUMATA Masahiro, NAKAMURA Toyoo

  日本畜産学会, 25 Apr. 1998, Animal science journal, 69(4) (4), 392 - 399, Japanese


• 畜産副生物の機能性食品用素材としての利用について

  本田 和久, 鈴木 美紀, 沼田 正寛, 中村 豊郎

  日本食肉研究会, 30 Jun. 1997, 食肉の科学, 38(1) (1), 184 - 188, Japanese

• 延髄のp38 MAPKのリン酸化はニワトリヒナにおける食後の満腹感の誘導に関与するか？

  改發美友, 竹垣淳也, 本田和久, 實安隆興

  日本家禽学会2025年度春季大会, Mar. 2025, Japanese

  Oral presentation


• 玄米主体低窒素飼料へのアミノ酸添加がブロイラーの成長成績に及ぼす影響

  右田梨紗, 竹垣淳也, 實安隆興, 本田和久

  日本家禽学会2025年度春季大会, Mar. 2025, Japanese

  Oral presentation


• 高張液投与がニワトリのタンパク質・脂質代謝関連遺伝子の発現に及ぼす影響

  村田薫子, ZHANG Yuhui, 竹垣淳也, 實安隆興, 本田和久

  日本家禽学会2025年度春季大会, Mar. 2025, Japanese

  Oral presentation


• 飢えと渇きからの自由を摂食・飲水調節機構から考える

  本田 和久

  第48回鳥類内分泌研究会, Nov. 2024, Japanese

  [Invited]

  Invited oral presentation


• 夜間青色照明の点灯が産卵鶏の摂食と産卵に及ぼす影響

  宮下基, 實安隆興, 本田和久

  日本畜産学会第132回大会, Sep. 2024, Japanese

  Oral presentation


• 地産地消を実現する地鶏肉生産の取り組み

  本田 和久

  日本家禽学会2024年度春季大会公開シンポジウム「持続可能な食料生産システムに向けた戦略～環境にやさしい食品産業の挑戦～」, Mar. 2024, Japanese

  Public symposium


• 食欲抑制ホルモンの中枢投与や絶食・再給餌がニワトリ中枢の炎症性サイトカインの遺伝子発現に及ぼす影響

  北山藍香, 本田和久, 實安隆興

  令和5年度第73回関西畜産学会大会, Nov. 2023, Japanese

  Oral presentation


• L-137の給与がブロイラーのLPS誘導性の反応に及ぼす影響

  坊ケ内恵麻, 實安隆興, 本田和久

  令和5年度第73回関西畜産学会大会, Nov. 2023, Japanese

  Oral presentation


• 照明色を用いたブロイラーの移動行動の誘導が産肉量、肉質、及び脚の発達に及ぼす影響

  楠田慎吾, 大島用三, 實安隆興, 本田和久

  令和5年度第73回関西畜産学会大会, Nov. 2023, Japanese

  Oral presentation


• 玄米酒粕飼料の給与期間の違いがブロイラーの成長成績と肉質に及ぼす影響

  西川幸樹, 清水綾香, 平井猛博, 實安隆興, 本田和久

  令和5年度第73回関西畜産学会大会, Nov. 2023, Japanese

  Oral presentation


• 稲作を基盤とした地産地消の国産鶏肉生産に向けた取り組み

  本田和久

  令和5年度第73回関西畜産学会大会公開シンポジウム, Nov. 2023, Japanese

  [Invited]

  Nominated symposium


• 玄米主体飼料における酒粕の配合割合の違いがブロイラーの成長成績と肉色に及ぼす影響

  本田和久, 西川幸樹, 清水綾香, 平井猛博, 實安隆興

  日本家禽学会2023年度秋季大会, Sep. 2023, Japanese

  Oral presentation


• 玄米酒粕主体飼料の産卵鶏への給与が鶏卵の卵質に及ぼす影響

  本田和久, 清水綾香, 西川幸樹, 平井猛博, 實安隆興

  日本畜産学会第131回大会, Sep. 2023, Japanese

  Oral presentation


• 種々の神経ペプチドの中枢投与がニワトリヒナの飲水に及ぼす影響

  本田和久, Yuhui Zhang, 松波知弥, 谷口悠二, 樋本清一, 黒木悟, 實安隆興

  日本家禽学会2023年度春季大会（, Mar. 2023

  Oral presentation


• 飼養管理による高付加価値国産鶏肉の開発

  本田和久

  日本家禽学会2023年度春季大会公開シンポジウム「高付加価値な国産鶏肉生産への挑戦」, Mar. 2023

  Public symposium


• 玄米と酒粕の給与がブロイラーの肉質に及ぼす影響

  本田和久, 西川幸樹, 宮﨑香綸, 平井猛博, 實安隆興

  第64回日本食肉科学会大会, Mar. 2023

  Oral presentation


• インスリン様成長因子（IGF）-1 がニワトリ筋管細胞の IGF 関連遺伝子の発現に及ぼす影響

  本田和久, 長田晴季, 實安隆興

  日本家禽学会2022年度秋季大会, Sep. 2022, Japanese

  Oral presentation


• 照明を用いたブロイラーの移動行動の誘導

  楠田慎吾, 大島用三, 實安隆興, 本田和久

  日本家禽学会2022年度秋季大会, Sep. 2022, Japanese

  Oral presentation


• L-137 の給与がブロイラーの腸管免疫関連遺伝子の発現に及ぼす影響

  坊ケ内恵麻, 三山景子, 實安隆興, 本田和久

  日本家禽学会2022年度秋季大会, Sep. 2022, Japanese

  Oral presentation


• 飼料と水の混合給与がブロイラーの飲水量と浸透圧調節関連因子に及ぼす影響

  本田和久, 松波知弥, 谷口悠二, 樋本清一, 黒木 悟, 實安隆興, 上曽山博

  日本家禽学会2022年度春季大会, Mar. 2022, Japanese

  Oral presentation


• 玄米と酒粕の給与がブロイラーの成長成績と肉質に及ぼす影響

  西川幸樹, 宮﨑香綸, 本田和久, 平井猛博, 広畑修二, 實安隆興, 上曽山博

  日本家禽学会2022年度春季大会, Mar. 2022, Japanese

  Oral presentation


• 照明色の周期的・局所的切替えがブロイラーの行動に及ぼす影響

  楠田慎吾, 中村恒志, 本田和久, 大島用三, 實安隆興, 上曽山博

  日本家禽学会2022年度春季大会, Mar. 2022, Japanese

  Oral presentation


• ニワトリの摂食調節における視床下部 TGF-β/Smad シグナリングの役割に関する研究

  上田実来, 永田加菜美, 本田和久, 上曽山博, 實安隆興

  日本家禽学会2022年度春季大会, Mar. 2022, Japanese

  Oral presentation


• 玄米の給与期間の違いがブロイラー及び地鶏の肉質に及ぼす影響

  宮崎香綸, アルナ, 實安隆興, 本田和久, 上曽山博

  第71回関西畜産学会大会, Oct. 2021


• 高付加価値国産鶏肉研究開発プラットフォームのご紹介

  本田 和久

  第71回関西畜産学会大会, Oct. 2021

  [Invited]


• 飼料と水の混合給餌がブロイラーの産肉量と肉質に及ぼす影響

  松波知弥, 本田和久, 谷口悠二, 樋本清一, 黒木悟, 實安隆興, 上曽山博

  日本家禽学会2021年度春季大会, Mar. 2021, Japanese

  Oral presentation


• 絶食がニワトリの肝臓及び骨格筋のインスリン様成長因子結合タンパク質の発現に及ぼす影響

  長田晴季, 本田和久, Ahmed Kewan, 實安隆興, 上曽山博

  日本家禽学会2021年度春季大会, Mar. 2021, Japanese

  Oral presentation


• IGF-1のノックダウンがニワトリ筋管細胞のAkt及びErkのリン酸化に及ぼす影響

  中村友紀, 本田和久, 上曽山博, 實安隆興

  日本家禽学会2021年度春季大会, Mar. 2021, Japanese

  Oral presentation


• 論文査読の仕方～日本家禽学会編集委員長からのお願い～

  本田 和久

  日本家禽学会2021年度春季大会, Mar. 2021, Japanese

  [Invited]

  Invited oral presentation


• 加熱殺菌された乳酸菌L-137の給与がブロイラーの成長成績に及ぼす影響

  本田和久, 三山景子, 實安隆興, 上曽山博

  日本家禽学会2020年度春季大会, Mar. 2020, Japanese

  Oral presentation


• 青色照明が地鶏の時計遺伝子Bmal 1の発現に及ぼす影響

  中村恒志, 實安隆興, 本田和久, 上曽山博

  日本家禽学会2020年度春季大会, Mar. 2020, Japanese

  Oral presentation


• 玄米の給与が種々の肉用鶏の肉質に及ぼす影響

  宮下基, 田中真由子, 井通貫太, 實安隆興, 本田和久, 上曽山博

  日本家禽学会2020年度春季大会, Mar. 2020, Japanese

  Oral presentation


• 小豆タンパク質の給与が高脂肪食給与マウスの糖・脂質代謝に及ぼす影響

  坂本奈津子,森永涼司,實安隆興,本田和久,上曽山博

  第58回日本栄養・食糧学会近畿支部大会, Nov. 2019


• 青色照明の半日点灯がブロイラーの摂食量及び間脳・肝臓・骨格筋における種々の遺伝子発現に及ぼす影響

  本田和久,平本大地,近藤真,實安隆興,上曽山博

  日本畜産学会第126回大会, Sep. 2019


• 日齢及び性別の違いが地鶏の肉質に及ぼす影響

  HONDA KAZUHISA, 宮下 基, 田中 真由子, 實安 隆興, 上曽山 博

  日本畜産学会第125回大会, Mar. 2019, Japanese, 麻布大学, Domestic conference

  Oral presentation


• 玄米主体飼料の給与がブロイラー及び地鶏の産肉量と肉質に及ぼす影響

  HONDA KAZUHISA, 宮下 基, 田中 真由子, 實安 隆興, 上曽山 博

  日本家禽学会2019年度春季大会, Mar. 2019, Japanese, 麻布大学, Domestic conference

  Oral presentation


• Effect of myostatin on atrogin-1 expression in chicken myotubes

  SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2018年度秋季大会, Sep. 2018, Japanese, 東北大学, Domestic conference

  Oral presentation


• ニワトリにおいて、延髄Erkシグナリングは摂食抑制に関与している

  上野 瑞城, 福造 哲, HONDA KAZUHISA, KAMISOYAMA HIROSHI, SANEYASU TAKAOKI

  平成30年度第68回関西畜産学会大会, Sep. 2018, Japanese, 徳島大学, Domestic conference

  Oral presentation


• インスリンの中枢投与がブロイラーの視床下部Akt/FOXO及びPOMCの遺伝子発現に及ぼす影響

  福造 哲, 上野 瑞城, HONDA KAZUHISA, KAMISOYAMA HIROSHI, SANEYASU TAKAOKI

  平成30年度第68回関西畜産学会大会, Sep. 2018, Japanese, 徳島大学, Domestic conference

  Oral presentation


• 飼料用米の給与がブロイラーの産肉量及び肉質に及ぼす影響

  HONDA KAZUHISA, 平本 大地, 宮下 基, 實安 隆興, 上曽山 博

  日本畜産学会第124回大会, Mar. 2018, Japanese, 東京大学, Domestic conference

  Oral presentation


• 飼料用米の給与がひょうご味どりの産肉量及び肉質に及ぼす影響

  HONDA KAZUHISA, 平本 大地, 宮下 基, 實安 隆興, 上曽山 博

  日本家禽学会2018年度春季大会, Mar. 2018, Japanese, 東京大学, Domestic conference

  Oral presentation


• 小豆給与と運動の負荷が高脂肪食給与マウスの腹部脂肪蓄積に及ぼす影響

  森永 涼司, 田村 翔平, 實安 隆興, 本田 和久, 上曽 山博

  第56回日本栄養・食糧学会近畿支部大会, Nov. 2017, Japanese, Domestic conference

  Oral presentation


• 酵素合成グリコーゲンの給与はマウスの盲腸におけるグルカゴン様ペプチ

  田村 翔平, 森永 涼司, 安原 亜希, 實安 隆興, HONDA KAZUHISA, 上曽山 博

  第56回日本栄養・食糧学会近畿支部大会, Nov. 2017, Japanese, 龍谷大学, Domestic conference

  Oral presentation


• 絶食がニワトリの種々の骨格筋におけるタンパク質合成に及ぼす影響の比較

  YUMA NAKANO, NAMI TSUCHII, SATOSHI FUKUZO, HONDA KAZUHISA, KAMISOYAMA HIROSHI, SANEYASU TAKAOKI

  平成29年度 第67回関西畜産学会大阪大会, Sep. 2017, Japanese, 大阪府立大学, Domestic conference

  Oral presentation


• 飼料摂取がブロイラーとレイヤーにおける消化管ホルモンの遺伝子発現に及ぼす影響

  HONDA KAZUHISA, 中山 駿吾, 奥田 実加, 實安 隆興, 上曽山 博

  第123回日本畜産学会大会, Sep. 2017, Japanese, 信州大学, Domestic conference

  Oral presentation


• ニワトリの摂食調節機構におけるアグーチシグナリングタンパクの役割

  HONDA KAZUHISA, 牛 萌, 青木 昂史, 實安 隆興, 上曽山 博

  日本家禽学会2017年度秋季大会, Sep. 2017, Japanese, 信州大学, Domestic conference

  Oral presentation


• ニワトリにおいて、視床下部インスリンシグナル伝達経路は摂食抑制に関与している

  SATOSHI FUKUZO, SHOICHI FUJITA, AYANA KITASHIRO, MIZUKI UENO, YUMA NAKANO, HONDA KAZUHISA, KAMISOYAMA HIROSHI, SANEYASU TAKAOKI

  平成29年度 第67回関西畜産学会大阪大会, Sep. 2017, Japanese, 大阪府立大学, Domestic conference

  Oral presentation


• インスリンの中枢投与や再給餌がブロイラーの視床下部インスリンシグナル伝達経路に及ぼす影響

  SATOSHI FUKUZO, MIZUKI UENO, YUMA NAKANO, HONDA KAZUHISA, KAMISOYAMA HIROSHI, SANEYASU TAKAOKI

  日本家禽学会2017年度秋季大会, Sep. 2017, Japanese, 日本家禽学会, 信州大学, Domestic conference

  Oral presentation


• 酵素合成グリコーゲンが脂肪負荷マウスの血中トリグリセリド濃度及び高脂肪食給与マウスの腸内細菌叢に及ぼす影響

  田村 翔平, 森永 涼司, 實安 隆興, 本田 和久, 上曽山 博

  第71回日本栄養・食料学会大会, May 2017, Japanese, 沖縄コンベンションセンター, Domestic conference

  Poster presentation


• 種々のホルモンがニワトリの肝臓及び脂肪組織における脂質代謝関連遺伝子の発現に及ぼす影響

  髙木 聖子, 杉本 はるか, 實安 隆興, HONDA KAZUHISA, 上曽山 博

  第40回鳥類内分泌研究会大会, Nov. 2016, Japanese, 鳥類内分泌研究会, ホテルメルパルク松山（愛媛県松山市）, Domestic conference

  Oral presentation


• インスリン様成長因子及びアイリシンの中枢投与がニワトリの摂食に及ぼす影響

  HONDA KAZUHISA, 藤田 昇一, 奥田 実加, 實安 隆興, 上曽山 博

  第40回鳥類内分泌研究会大会, Nov. 2016, Japanese, 鳥類内分泌研究会, ホテルメルパルク松山（愛媛県松山市）, Domestic conference

  Oral presentation


• 短時間の絶食がブロイラーの脂肪組織トリグリセリド代謝関連遺伝子の発現に及ぼす影響

  髙木 聖子, 杉本 はるか, 實安 隆興, HONDA KAZUHISA, 上曽山 博

  第66回関西畜産学会大会, Oct. 2016, Japanese, 関西畜産学会, 香川大学農学部（香川県高松市）, Domestic conference

  Oral presentation


• 青色照明がブロイラーの肝臓及び骨格筋における時計遺伝子の発現に及ぼす影響

  平本 大地, 近藤 真, 實安 隆興, HONDA KAZUHISA, 上曽山 博

  第66回関西畜産学会大会, Oct. 2016, Japanese, 関西畜産学会, 香川大学農学部（香川県高松市）, Domestic conference

  Oral presentation


• 青色照明がニワトリの時計遺伝子の発現に及ぼす影響

  近藤真, 平本大地, 實安隆興, HONDA KAZUHISA, 上曽山博

  日本家禽学会2016年度秋季大会, Sep. 2016, Japanese, 日本家禽学会, 静岡県総合研修所もくせい会館（静岡県静岡市）, Domestic conference

  Oral presentation


• インスリン様成長因子-2の中枢投与はニワトリヒナの摂食を抑制する

  HONDA KAZUHISA, 牛 萌, 平本 大地, 藤田 昇一, 奥田 実加, 實安 隆興, 上曽山 博

  日本家禽学会2016年度秋季大会, Sep. 2016, Japanese, 日本家禽学会, 静岡県総合研修所もくせい会館（静岡県静岡市）, Domestic conference

  Oral presentation


• インスリン様成長因子-1の中枢投与はニワトリヒナの摂食を抑制する

  平本大地, 牛萌, 藤田昇一, 奥田実加, 實安隆興, HONDA KAZUHISA, 上曽山博

  日本家禽学会2016年度秋季大会, Sep. 2016, Japanese, 日本家禽学会, 静岡県総合研修所もくせい会館（静岡県静岡市）, Domestic conference

  Oral presentation


• Regulation of feed intake and body fat mass in chickens

  Kazuhisa Honda

  XXV World's Poultry Congress, Sep. 2016, English, The World's Poultry Science Association, China National Convention Center（中国北京市）, International conference

  [Invited]

  Invited oral presentation


• ニワトリの食欲調節機構に関するこれまでの研究と今後の展望

  HONDA KAZUHISA

  第71回岡山実験動物研究会例会, Jun. 2016, Japanese, 岡山実験動物研究会, 就実大学（岡山県岡山市）, Domestic conference

  [Invited]

  Invited oral presentation


• 牛肉エキスエタノール抽出画分の給与がラットの腹部脂肪蓄積に及ぼす影響

  KAZUHISA HONDA, TAKAOKI SANEYASU, HIROSHI KAMISOYAMA

  第70回日本栄養・食糧学会大会, May 2016, Japanese, 公益社団法人日本栄養・食糧学会, 武庫川女子大学, Domestic conference

  Oral presentation


• 肉用鶏の生産性を高める鶏舎LED光線管理システムの開発

  HONDA KAZUHISA

  日本家禽学会2016年度春季大会, Mar. 2016, Japanese, 日本獣医生命科学大学, Domestic conference

  Public discourse


• 短時間の絶食がブロイラーの骨格筋におけるタンパク質代謝関連因子の発現量に及ぼす影響

  TSUCHII NAMI, KITASHIRO AYANA, TSUCHIHASHI TATSUYA, SHIDO HARUKA, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2016年度春季大会, Mar. 2016, Japanese, 日本獣医生命科学大学, Domestic conference

  Oral presentation


• 新生ヒナの骨格筋におけるタンパク質合成関連因子の発現変動

  TSUCHIHASHI TATSUYA, KIMURA SAYAKA, KITASHIRO AYANA, TSUCHII NAMI, SHINDO HARUKA, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2016年度春季大会, Mar. 2016, Japanese, 日本獣医生命科学大学, Domestic conference

  Oral presentation


• ニワトリにおけるインスリンによる摂食抑制に視床下部mTORは関与していない

  KITASHIRO AYANA, TSYCHII NAMI, TSUCHIHASHI TATSUYA, SHIDO HARUKA, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2016年度春季大会, Mar. 2016, Japanese, 日本獣医生命科学大学, Domestic conference

  Oral presentation


• Peptide YY functions as an anorexgenic peptide in the peripheral circulation in chicks

  Koji Aoki, Tomohiko Shimatani, Meng Niu, Makoto Kondo, Mika Okuda, Takaoki Saneyasu, Kazuhisa Honda, Hiroshi Kamisoyama

  第40回日本比較内分泌学会大会, Dec. 2015, Japanese, 日本比較内分泌学会, JMSアステールプラザ（広島県広島市）, Domestic conference

  Poster presentation


• 水溶性ヘム鉄の給与と運動の負荷が高脂肪食給与マウスの脂肪組織におけるトリグリセリド代謝及び炎症関連遺伝子の発現に及ぼす影響

  勝村仁智, 實安隆興, 本田 和久, 上曽山博

  第54回日本栄養・食糧学会近畿支部大会, Oct. 2015, Japanese, 日本栄養・食糧学会近畿支部, 神戸大学, Domestic conference

  Oral presentation


• 酵素合成グリコーゲンの給与と運動の負荷が高脂肪食給与マウスに及ぼす影響

  田村翔平, 勝村仁智, 實安隆興, 本田 和久, 上曽山博

  第54回日本栄養・食糧学会近畿支部大会, Oct. 2015, Japanese, 日本栄養・食糧学会近畿支部, 神戸大学, Domestic conference

  Oral presentation


• 酵素合成グリコーゲンの給与が高脂肪食肥満マウスの種々の臓器・組織における遺伝子発現に及ぼす影響

  本田 和久, 小川瑠衣, 古屋敷隆, 高田洋樹, 保田倫子, 上曽山博, 芦田均

  第54回日本栄養・食糧学会近畿支部大会, Oct. 2015, Japanese, 日本栄養・食糧学会近畿支部, 神戸大学, Domestic conference

  Oral presentation


• 発光ダイオード照明の点灯時間の違いがブロイラーの成長に及ぼす影響

  近藤真, 平本大地, 實安隆興, 本田 和久, 上曽山博

  日本家禽学会2015年度秋季大会, Sep. 2015, Japanese, 日本家禽学会, 酪農学園大学, Domestic conference

  Oral presentation


• 短期間のコルチコステロン給与が新生ヒナに及ぼす影響

  大屋華, 近藤真, 平本大地, 實安隆興, 本田 和久, 上曽山博

  日本家禽学会2015年度秋季大会, Sep. 2015, Japanese, 日本家禽学会, 酪農学園大学, Domestic conference

  Oral presentation


• 青色発光ダイオード照明がニワトリヒナの骨格筋形成或いはタンパク質代謝関連遺伝子の発現に及ぼす影響

  近藤真, 平本大地, 實安隆興, 本田 和久, 上曽山博

  日本畜産学会第120回大会, Sep. 2015, Japanese, 日本畜産学会, 酪農学園大学, Domestic conference

  Oral presentation


• 水溶性ヘム鉄の給与と運動の負荷が高脂肪食給与マウスに及ぼす影響

  勝村仁智, 實安隆興, 本田和久, 上曽山博

  平成27年度（第65回）関西畜産学会大会, Sep. 2015, Japanese, 関西畜産学会, 愛媛大学, Domestic conference

  Oral presentation


• 飼料へのコルチコステロンの添加がニワトリヒナの骨格筋の形成或いはタンパク質代謝関連遺伝子の発現に及ぼす影響

  大屋華, 近藤真, 平本大地, 實安隆興, 本田 和久, 上曽山博

  日本畜産学会第120回大会, Sep. 2015, Japanese, 日本畜産学会, 酪農学園大学, Domestic conference

  Oral presentation


• コルチコステロンはニワトリの脂肪組織トリグリセリドリパーゼ遺伝子の発現を促進する

  髙木聖子, 杉本はるか, 倉地清隆, 青木達哉, 實安隆興, 本田和久, 上曽山博

  平成27年度（第65回）関西畜産学会大会, Sep. 2015, Japanese, 関西畜産学会, 愛媛大学, Domestic conference

  Oral presentation


• 青色発光ダイオード照明がニワトリヒナの血中コルチコステロン濃度に及ぼす影響

  HONDA KAZUHISA, MAKOTO KONDO, MIKA OKUDA, SANEYASU TAKAOKI, KAMISOYAMA HIROSHI

  日本家禽学会2015年度春季大会, Mar. 2015, Japanese, 日本家禽学会, 宇都宮大学, Domestic conference

  Oral presentation


• 種々のニワトリの骨格筋における脂質代謝特性の比較

  SAYAKA KIMURA, AYANA KITASHIRO, TATSUYA TSUCHIHASHI, NAMI TSUCHII, MARIKO INUI, SANEYASU TAKAOKI, KAZUHISA HONDA, HIROSHI KAMISOYAMA

  日本畜産学会第119回大会, Mar. 2015, Japanese, 日本畜産学会, 宇都宮大学, Domestic conference

  Oral presentation


• ブロイラーにおける骨格筋タンパク質合成関連因子の遺伝子発現量の成長に伴う変化

  SAYAKA KIMURA, MARIKO INUI, TATSUYA TSUCHIHASHI, AYANA KITASHIRO, NAMI TSUCHII, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2015年度春季大会, Mar. 2015, Japanese, 日本家禽学会, 宇都宮大学, Domestic conference

  Oral presentation


• ニワトリにおけるグルカゴン様ペプチド-1及び2の摂食抑制機構へのAMPKの関与

  TOMOHIKO SHIMATANI, KOJI AOKI, 牛 萌, TAKUYA YAMGUCHI, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2015年度春季大会, Mar. 2015, Japanese, 日本家禽学会, 宇都宮大学, Domestic conference

  Oral presentation


• グルカゴン様ペプチド-2 の摂食抑制効果はエキセンジン（5-39）によって緩和される

  TOMOHIKO SHIMATANI, KOJI AOKI, 牛 萌, TAKUYA YAMGUCHI, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本畜産学会第119回大会, Mar. 2015, Japanese, 日本畜産学会, 宇都宮大学, Domestic conference

  Oral presentation


• 機能性素材を用いた畜産物の生産性向上と品質改善

  HONDA KAZUHISA

  第44回日本栄養・食糧学会北海道支部会, Nov. 2014, Japanese, 北海道大学, Domestic conference

  [Invited]

  Nominated symposium


• 絶食がブロイラーの浅胸筋及び長内転筋におけるタンパク質代謝関連遺伝子の発現に及ぼす影響

  KIMURA SAYAKA, INUI MARIKO, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2014年度秋季大会, Sep. 2014, Japanese, 鹿児島大学, Domestic conference

  Oral presentation


• 水溶性ヘム鉄の給与が高脂肪食給与マウスの脂質代謝に及ぼす影響

  KATSUMURA MASANORI, SUGIMOTO HARUKA, KURACHI KIYOTAKA, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  平成26年度（第64回）関西畜産学会大会, Sep. 2014, Japanese, 広島大学, Domestic conference

  Oral presentation


• ニワトリ肝臓脂肪酸酸化関連遺伝子の発現調節におけるペルオキシソーム増殖剤活性化受容体αの役割

  SUGIMOTO HARUKA, KURACHI KIYOTAKA, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2014年度秋季大会, Sep. 2014, Japanese, 鹿児島大学, Domestic conference

  Oral presentation


• ニワトリにおける視床下部摂食調節ペプチドの種々のパラメーターとの関係

  AOKI KOJI, SHIMATANI TOMOHIKO, YAMAGUCHI TAKUYA, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2014年度秋季大会, Sep. 2014, Japanese, 鹿児島大学, Domestic conference

  Oral presentation


• グルカゴン様ペプチド-1及び-2の静脈内投与がニワトリヒナの摂食に及ぼす影響

  SHIMATANI TOMOHIKO, AOKI KOJI, YAMAGUCHI TAKUYA, SANEYASU TAKAOKI, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2014年度秋季大会, Sep. 2014, Japanese, 鹿児島大学, Domestic conference

  Oral presentation


• 絶食がレイヤー及びブロイラーの骨格筋タンパク質代謝関連遺伝子の発現に及ぼす影響

  SANEYASU TAKAOKI, INUI MARIKO, KIMURA SAYAKA, YOSHIMOTO YU, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  日本家禽学会2014年度春季大会, Mar. 2014, Japanese, 日本家禽学会, 茨城県つくば市, Domestic conference

  Oral presentation


• ブロイラーの浅胸筋及び長内転筋における骨格筋タンパク質代謝関連因子の遺伝子発現の比較

  SANEYASU TAKAOKI, HONDA KAZUHISA, INUI MARIKO, YOSHIMOTO YU, KIMURA SAYAKA, KAMISOYAMA HIROSHI

  日本畜産学会第118回大会, Mar. 2014, Japanese, 日本畜産学会, 茨城県つくば市, Domestic conference

  Poster presentation


• グルカゴン様ペプチド-2の中枢投与はブロイラーヒナの摂食を抑制する

  HONDA KAZUHISA, SANEYASU TAKAOKI, YAMAGUCHI TAKUYA, AOKI KOJI, SHIMATANI TOMOHIKO, NAKANISHI KIWAKO, KAMISOYAMA HIROSHI

  日本家禽学会2014年度春季大会, Mar. 2014, Japanese, 日本家禽学会, 茨城県つくば市, Domestic conference

  Oral presentation


• オキシントモジュリンの中枢投与はブロイラーヒナの摂食を抑制する

  HONDA KAZUHISA, SANEYASU TAKAOKI, YAMAGUCHI TAKUYA, AOKI KOJI, SHIMATANI TOMOHIKO, NAKANISHI KIWAKO, KAMISOYAMA HIROSHI

  日本畜産学会第118回大会, Mar. 2014, Japanese, 日本畜産学会, 茨城県つくば市, Domestic conference

  Oral presentation


• 絶食及び再給餌がニワトリの脂肪組織脂質代謝関連遺伝子の発現に及ぼす影響

  白垣 美穂, 倉地 清隆, 本田 和久, 上曽山 博

  平成25年度第63回関西畜産学会大会, Sep. 2013, Japanese, 関西畜産学会, 滋賀県立大学, Domestic conference

  Oral presentation


• 新規グルカゴン様ペプチドの中枢投与はニワトリヒナの摂食を抑制する

  山口 拓也, 中西 貴和子, 嶋谷 朋彦, 青木 昂史, 本田 和久, 上曽山 博

  日本畜産学会第117回大会, Sep. 2013, Japanese, 日本畜産学会, 新潟大学, Domestic conference

  Oral presentation


• 酵素処理ヤシ粕（マンノクリーンβ）給与によるブロイラーの筋肉重量増加機構の解明

  吉本雄, 伊吹昌久, 福井健介, 米元博子, 乾真梨子, 本田 和久, 上曽山 博

  日本家禽学会2013年度秋季大会, Sep. 2013, Japanese, 日本家禽学会, 新潟大学, Domestic conference

  Oral presentation


• ブロイラーにおける筋肉タンパク質代謝関連遺伝子発現の成長に伴う変化

  吉本 雄, 乾 真梨子, 本田 和久, 上曽山博

  平成25年度第63回関西畜産学会大会, Sep. 2013, Japanese, 関西畜産学会, 滋賀県立大学, Domestic conference

  Oral presentation


• ニワトリオキシントモジュリンの脳室内投与はニワトリの摂食を抑制する

  山口 拓也, 中西 貴和子, 嶋谷 朋彦, 青木 昂史, 本田 和久, 上曽山 博

  平成25年度第63回関西畜産学会大会, Sep. 2013, Japanese, 関西畜産学会, 滋賀県立大学, Domestic conference

  Oral presentation


• グルカゴン様ペプチド-2の中枢投与はニワトリヒナの摂食を強力に抑制する

  山口 拓也, 中西 貴和子, 嶋谷 朋彦, 青木 昂史, 本田 和久, 上曽山 博

  日本家禽学会2013年度秋季大会, Sep. 2013, Japanese, 日本家禽学会, 新潟大学, Domestic conference

  Oral presentation


• 可視近赤外分光法を用いた卵黄コレステロール非破壊計測技術の開発

  山中 志保, 黒木 信一郎, HONDA KAZUHISA, KAMISOYAMA HIROSHI

  農業機械学会関西支部第129回例会, Mar. 2013, Japanese, 神戸, Domestic conference

  Oral presentation


• グルカゴン様ペプチド-1はグルコース負荷によるニワトリの血糖値上昇を抑制する

  HONDA KAZUHISA, 加藤 宏尚, 野川 泰典, 上曽山 博, 加藤 和雄, 長谷川 信

  日本家禽学会2013年度春季大会, Mar. 2013, Japanese, 日本家禽学会, 広島市, Domestic conference

  Oral presentation


• 二品種のニワトリの成長に伴う視床下部食欲調節関連神経ペプチドの遺伝子発現の比較

  中西 貴和子, 實安 隆興, 以倉 淳史, HONDA KAZUHISA, 長谷川 信, 上曽山 博

  日本家禽学会2012年度秋季大会, Sep. 2012, Japanese, 日本家禽学会, 香川県高松市, Domestic conference

  Oral presentation


• 絶食及び再給餌がニワトリの脂肪組織脂質代謝関連遺伝子の発現に及ぼす影響

  白垣 美穂, 中西 浩平, HONDA KAZUHISA, 長谷川 信, 上曽山 博

  日本家禽学会2012年度秋季大会, Sep. 2012, Japanese, 日本家禽学会, 香川県高松市, Domestic conference

  Oral presentation


• 絶食及び再給餌がニワトリの肝臓脂質代謝関連遺伝子の発現に及ぼす影響

  白垣 美穂, HONDA KAZUHISA, 中西 浩平, 長谷川 信, 上曽山 博

  第62回関西畜産学会大会, Sep. 2012, Japanese, 関西畜産学会, 和歌山市, Domestic conference

  Oral presentation


• 絶食及び再給餌がニワトリの肝臓コレステロール代謝関連遺伝子の発現に及ぼす影響

  朴 祐理子, 白垣 美穂, 中西 浩平, HONDA KAZUHISA, 長谷川 信, 上曽山 博

  日本家禽学会2012年度秋季大会, Sep. 2012, Japanese, 日本家禽学会, 香川県高松市, Domestic conference

  Oral presentation


• 酵素処理ヤシ粕（マンノクリーンβ）の給与がブロイラーの成長に及ぼす影響

  吉本 雄, 伊吹 昌久, 福井 健介, 米元 博子, 山崎 宏樹, HONDA KAZUHISA, 長谷川 信, 上曽山 博

  日本家禽学会2012年度秋季大会, Sep. 2012, Japanese, 日本家禽学会, 香川県高松市, Domestic conference

  Oral presentation


• 鶏胸筋におけるヒートショックタンパク質の発現解析

  上田修司, 小鍛治泰斗, 清水俊策, 桐村悠佑, HONDA KAZUHISA, 上曽山博, 白井康仁, 山之上稔

  第62回関西畜産学会大会, Sep. 2012, Japanese, 関西畜産学会, Domestic conference

  Oral presentation


• 甘草フラボノイドオイル給与による動物のコレステロール代謝改善

  朴 祐理子, 富永 雄仁, 横田 真一, HONDA KAZUHISA, 上曽山 博

  第62回関西畜産学会大会, Sep. 2012, Japanese, 関西畜産学会, 和歌山市, Domestic conference

  Oral presentation


• ブロイラーの腹腔内脂肪蓄積に及ぼす食餌大豆胚芽蛋白質の影響

  前田卓磨, 元木徹, 斎藤三四郎, 佐藤俊郎, 片岡久, 池上賢伍, 中山陽子, HONDA KAZUHISA, 上曽山 博, 長谷川 信

  日本家禽学会2012年度秋季大会, Sep. 2012, Japanese, 日本家禽学会, 香川県高松市, Domestic conference

  Oral presentation


• 食肉の日齢評価に利用可能なタンパク質マーカーの探索

  清水 俊策, 上田 修司, 桐村 悠佑, 本田 和久, 上曽山 博, 白井 康仁, 山之上 稔

  日本畜産学会第115回大会, Mar. 2012, Japanese, 名古屋大学, Domestic conference

  Oral presentation


• 醤油粕等発酵物の給与が鶏肉及び鶏卵生産に及ぼす影響

  朴 祐理子, 上曽山 博, 伊藤 丈直, 中井 美貴, 本田 和久, 長谷川 信

  日本家禽学会2012年度春季大会, Mar. 2012, Japanese, 名古屋大学, Domestic conference

  Oral presentation


• 食事誘導肥満マウスにおける酵素合成グリコーゲン摂取の効果

  OGAWA Rui, 古屋敷 隆FURUYASHIKI Takashi, NAKAYAMA Yoko, HONDA Kazuhisa, KAMISOYAMA Hiroshi, TAKATA Hiroki, YASUDA Michiko, ASHIDA Hitoshi

  第66回日本栄養・食糧学会大会, 2012, Japanese, 仙台, Domestic conference

  Oral presentation


• 産卵鶏へのキトサン給与が産卵成績と卵黄コレステロール濃度に及ぼす影響

  池上 賢伍, 千秋 正信, 上曽山 博, 本田 和久, 長谷川 信

  日本畜産学会第114回大会, Aug. 2011, Japanese, 北里大学, Domestic conference

  Poster presentation


• 鶏におけるグルカゴンの摂食抑制機構に関する研究

  中山 陽子, 実安 隆興, 桂 千広, 本田 和久, 上曽山 博, 長谷川 信

  日本畜産学会第114回大会, Aug. 2011, Japanese, 北里大学, Domestic conference

  Poster presentation


• α、β或いはγ-メラニン細胞刺激ホルモンの中枢投与が二品種の鶏の摂食量に及ぼす影響

  池上 賢伍, 実安 隆興, 本田 和久, 上曽山 博, 長谷川 信

  日本家禽学会2011年度秋季大会, Aug. 2011, Japanese, 北里大学, Domestic conference

  Oral presentation


• 種々のメラニン細胞刺激ホルモンの中枢投与がブロイラーの摂食量に及ぼす影響

  Yoko Nakayama, Takaoki Saneyasu, Kengo Ikegami, Kazuhisa Honda, Hiroshi Kamisoyama, Shin Hasegawa

  日本家禽学会2011年度春季大会, Mar. 2011, Japanese, 東京農業大学, Domestic conference

  Oral presentation


• 食餌性肥満マウスの脂質代謝に及ぼす酵素合成グリコーゲンの影響

  OGAWA Rui, FURUYASHIKI Takashi, NAKAYAMA Yoko, HONDA Kazuhisa, KAMISOYAMA Hiroshi, TAKATA Hiroki, YASUDA Michiko, ASHIDA Hitoshi

  2011年度日本農芸化学会関西・中部支部合同大会(関西支部第471回講演会), 2011, Japanese, Domestic conference

  Oral presentation


• 酵素合成グリコーゲンが食餌性肥満マウスの脂質代謝に及ぼす影響

  OGAWA Rui, FURUYASHIKI Takashi, NAKAYAMA Yoko, HONDA Kazuhisa, KAMISOYAMA Hiroshi, TAKATA Hiroki, YASUDA Michiko, ASHIDA Hitoshi

  神戸大学若手フロンティア研究会2011, 2011, Japanese, Domestic conference

  Poster presentation


• 酵素合成グリコーゲンが高脂肪食摂取ラットの脂質代謝に及ぼす影響

  OGAWA Rui, YASUDA Michiko, NAKAYAMA Yoko, HONDA Kazuhisa, KAMISOYAMA Hiroshi, FURUYASHIKI Takashi, TAKATA Hiroki, ASHIDA Hitoshi

  第65回日本栄養・食糧学大会, 2011, Japanese, Domestic conference

  Oral presentation


• Effect of enzymatically synthesized glycogen on fat accumulation and lipid metabolism in mice fed a high-fat diet

  OGAWA Rui, NAKAYAMA Yoko, HONDA Kazuhisa, KAMISOYAMA Hiroshi, FURUYASHIKI Takashi, TAKATA Hiroki, YASUDA Michiko, Ashida Hitoshi

  2011 International Conference on Food Factors (ICoFF2011), 2011, English, International conference

  Poster presentation


• ブロイラーにおけるPotassium diformate給与による筋肉量増加機構の解明

  Shinpei Kubo, Tohru Motoki, Hiroshi Kamisoyama, Kazuhisa Honda, Shin Hasegawa

  日本家禽学会2010年度秋季大会, Sep. 2010, Japanese, 信州大学, Domestic conference

  Oral presentation


• コエンザイムQ10給与による卵黄コレステロール減少の機構解明

  本告 友規, 本田 和久, 上曽山 博, 長谷川 信

  日本家禽学会2010年度秋季大会, Sep. 2010, Japanese, 信州大学, Domestic conference

  Oral presentation


• 培養肝細胞を用いた脂質代謝改善因子検索系の開発

  Tomoki Motoori, Kazuhisa Honda, Hiroshi Kamisoyama, Shin Hasegawa

  日本畜産学会第112回大会, Mar. 2010, Japanese, 明治大学, Domestic conference

  Oral presentation


• 培養肝細胞を用いた鶏の脂質代謝改善因子検索系の開発

  Tomoki Motoori, Kazuhisa Honda, Hiroshi Kamisoyama, Shin Hasegawa

  日本家禽学会2010年度春季大会, Mar. 2010, Japanese, 明治大学, Domestic conference

  Oral presentation


• 小豆の給与がラットの内臓脂肪蓄積に及ぼす影響

  Shinpei Kubo, Hiroshi Kamisoyama, Kazuhisa Honda, Shin Hasegawa

  日本畜産学会第112回大会, Mar. 2010, Japanese, 明治大学, Domestic conference

  Oral presentation


• 小豆の給与がニワトリの内臓脂肪蓄積に及ぼす影響

  Shinpei Kubo, Hiroshi Kamisoyama, Kazuhisa Honda, Shin Hasegawa

  日本家禽学会2010年度春季大会, Mar. 2010, Japanese, 明治大学, Domestic conference

  Oral presentation


• 種々の大豆粕加工品の給与が鶏の成長,、脂肪蓄積及び血中脂質成分に及ぼす影響

  Kazuhisa Honda, Kamisoyama Hiroshi, Shin Hasegawa

  日本食品科学工学会第56回大会, Sep. 2009, Japanese, 名城大学, Domestic conference

  Oral presentation


• 鶏におけるPotassium Diformateの筋肉量増加機構について

  Kazuhisa Honda, Takaoki Saneyasu, Hiroshi Itoh, Yasuo Odawara, Hiroshi Kamisoyama, Shin Hasegawa

  日本家禽学会2009年度秋季大会, Sep. 2009, Japanese, 琉球大学, Domestic conference

  Oral presentation


• 飼料中コエンザイムQ10の卵黄への移行

  Kazuhisa Honda, Kanako Kitaguchi, Hiroshi Kamisoyama, Shin Hasegawa

  日本家禽学会2009年度春季大会, Mar. 2009, Japanese, 日本家禽学会, 日本大学, Domestic conference

  Oral presentation


• 家禽飼料における抗菌性飼料添加物代替候補としての植物抽出物の有効性検討とその機構解明

  Ryuichi Yoshizaki, Hiroshi Ito, Yasuo Odawara, Kazuhisa Honda, Hiroshi Kamisoyama, Shin Hasegawa

  日本家禽学会2009年度春季大会, Mar. 2009, Japanese, 日本大学, Domestic conference

  Oral presentation


• 甘草フラボノイドオイルの給与が食餌性肥満ラットの腹腔内脂肪蓄積に及ぼす影響

  Hiroshi Kamisoyama, Kazuhisa Honda, Shusuke Yamasaki, Yuji Tominaga, Shinich Yokota, Shin Hasegawa

  第62回日本栄養・食糧学会大会, May 2008, Japanese, 日本栄養・食糧学会, 女子栄養大学, Domestic conference

  Oral presentation


• The functional roles of glucagon family in the central regulation of food intake in chicks

  Shin Hasegawa, Kazuhisa Honda, Hiroshi Kamisoyama

  1st Mediterranean Summit of WPSA, May 2008, English, Greece, International conference

  Poster presentation


• コエンザイムQ10の給与が鶏の血漿及び卵黄コレステロールに及ぼす影響

  北口 可奈子, 吉原 勢氏郎, 本田 和久, 上曽山 博, 長谷川 信

  日本畜産学会第109回大会, Mar. 2008, Japanese, 常磐大学, Domestic conference

  Oral presentation


• 有害細菌低減効果或いは免疫賦活効果を有する新規飼料添加物の開発

  北口 可奈子, 伊藤 博史, 小田原 保男, 本田 和久, 上曽山 博, 長谷川 信

  日本畜産学会第108回大会, Sep. 2007, Japanese, 岡山大学, Domestic conference

  Oral presentation


• Effects of plant by-products and organic acids on performance of growing chicks

  Shin Hasegawa, Hiroshi Ito, Yasuo Odawara, Kazuhisa Honda, Hiroshi Kamisoyama

  10th Asian Congress of Nutrition, Sep. 2007, English, Taipei, International conference

  Poster presentation


• 種々の植物性副産物の給与が鶏の脂質代謝に及ぼす影響

  北口 可奈子, 伊藤 博史, 福間 義教, 小田原 保男, 本田 和久, 上曽山 博, 長谷川 信

  日本畜産学会第107回大会, Mar. 2007, Japanese, 麻布大学, Domestic conference

  Oral presentation


• 鶏におけるニューロメジンUの摂食抑制機構に関する研究

  本田 和久, 多胡 健太郎, 上曽山 博, 長谷川 信

  日本畜産学会第107回大会, Mar. 2007, Japanese, 麻布大学, Domestic conference

  Oral presentation


• 鶏におけるグルカゴンの摂食抑制機構に関する研究

  Kazuhisa Honda

  日本家禽学会2006年度春季大会, Mar. 2006, Japanese, 九州大学, Domestic conference

  Oral presentation


• 鶏におけるPotassium Diformateの生理的機能に関する研究

  Hiroshi Kamisoyama, Kazuhisa Honda, 山崎 周介, 長谷川 信

  日本家禽学会2006年度春季大会, Mar. 2006, Japanese, 九州大学, Domestic conference

  Oral presentation


• ２品種の鶏における視床下部ニューロペプチドY の発現量の比較，

  本田 和久, 実安 隆興, 林 玲子, 熱田 博之, 植村 卓, 上曽山 博, 長谷川 信

  第104 回日本畜産学会大会, 2005, Japanese, 不明, 不明, Domestic conference

  Oral presentation


• 大豆タンパク質中の体脂肪蓄積抑制因子の検索

  戸田 真未, 上曽山 博, 本田 和久, 長谷川 信, 元木 徹

  第103回日本畜産学会, 2004, Japanese, 日本畜産学会, 未記入, Domestic conference

  Oral presentation


• ニューロペプチドＹの中枢投与が鶏の摂食量に及ぼす影響

  実安 隆興, 本田 和久, 上曽山 博, 長谷川 信

  第103回日本畜産学会, 2004, Japanese, 日本畜産学会, 未記入, Domestic conference

  Oral presentation


• 種々のホルモンがニワトリの肝臓及び脂肪組織における脂質代謝関連遺伝子の発現に及ぼす影響

  髙木聖子, 杉本はるか, SANEYASU Takaoki, HONDA Kazuhisa, 上曽山博, KAMISOYAMA Hiroshi

  第40回鳥類内分泌研究会大会, Nov. 2001, Japanese, 鳥類内分泌研究会, ホテルメルパルク松山, Domestic conference

  Oral presentation


• インスリン様成長因子及びアイリシンの中枢投与がニワトリの摂食に及ぼす影響

  HONDA Kazuhisa, 藤田昇一, 奥田実加, SANEYASU Takaoki, 上曽山博, KAMISOYAMA Hiroshi

  第40回鳥類内分泌研究会大会, Nov. 2001, Japanese, 鳥類内分泌研究会, ホテルメルパルク松山, Domestic conference

  Oral presentation


• 短時間の絶食がブロイラーの脂肪組織トリグリセリド代謝関連遺伝子の発現に及ぼす影響

  髙木聖子, 杉本はるか, SANEYASU Takaoki, HONDA Kazuhisa, 上曽山博, KAMISOYAMA Hiroshi

  第66回関西畜産学会大会, Oct. 2001, Japanese, 関西畜産学会, 香川大学農学部, Domestic conference

  Oral presentation


• 青色照明がブロイラーの肝臓及び骨格筋における時計遺伝子の発現に及ぼす影響

  平本大地, 近藤真, SANEYASU Takaoki, HONDA Kazuhisa, 上曽山博, KAMISOYAMA Hiroshi

  第66回関西畜産学会大会, Oct. 2001, Japanese, 関西畜産学会, 香川大学農学部, Domestic conference

  Oral presentation


• インスリン様成長因子-2の中枢投与はニワトリヒナの摂食を抑制する

  HONDA Kazuhisa, 牛萌, 平本大地, 藤田昇一, 奥田実加, SANEYASU Takaoki, 上曽山博, KAMISOYAMA Hiroshi

  日本家禽学会2016年度秋季大会, Sep. 2001, Japanese, 日本家禽学会, 静岡県総合研修所もくせい会館, Domestic conference

  Oral presentation


• Regulation of feed intake and body fat mass in chickens

  HONDA Kazuhisa

  XXV World's Poultry Congress, Sep. 2001, English, The World's Poultry Science Association, China National Convention Center, International conference

  [Invited]

  Invited oral presentation

• 日本栄養・食糧学会

  Apr. 2014 - Mar. 2025


• 日本食肉科学会

  Apr. 2010 - Mar. 2025


• 関西畜産学会


• 日本家禽学会


• 日本畜産学会

• 鳥類特異的な飲水調節機構の解明～進化の過程で獲得した渇きに耐えるための秘策～

  本田 和久, 横山 俊史, 佐藤 幹

  日本学術振興会, 科学研究費助成事業, 基盤研究(B), 神戸大学, Apr. 2024 - Mar. 2027, Principal investigator


• 稲作を基盤とした家禽用国産配合飼料開発事業

  公益財団法人全国競馬・畜産振興会, 公益財団法人全国競馬・畜産振興会畜産振興事業, 神戸大学, May 2022 - Mar. 2024, Principal investigator


• インスリン様成長因子結合タンパク質遺伝子の発現調節による革新的食肉生産技術の開発

  本田 和久

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Kobe University, Apr. 2021 - Mar. 2024, Principal investigator

  本研究では、ニワトリの骨格筋及び脂肪組織において発現するIGFBPの種類とその栄養状態に応じた発現変動を明らかにする目的で、浅胸筋、大内転筋、長内転筋、腓腹筋、縫工筋、大腿二頭筋及び腹腔内脂肪組織において発現するIGFBPsのmRNA量の種類と、絶食がニワトリの浅胸筋、大腿二頭筋及び腹部脂肪組織におけるIGFBPsのmRNA量に及ぼす影響、およびin vitro条件下で筋管細胞と前駆脂肪細胞における種々の核内転写因子のアゴニストあるいは栄養素が、IGFBP遺伝子のmRNA量に及ぼす影響を調べた。その結果、調べたすべての骨格筋において、IGFBP-1～5のmRNAが検出されたが、浅胸筋におけるIGFBP-1および2のmRNA量は、その他の部位に比べて低い傾向を示した。一方、脂肪組織においては、IGFBP-1～3のmRNAのみ検出された。これらの結果から、IGFBPの生理的役割は、骨格筋の部位や脂肪組織で異なることが示唆された。また、絶食は、骨格筋におけるIGFBP-3のmRNA量を減少させ、IGFBP-1のmRNA量を増加させること、脂肪組織においては、IGFBP-1および3のmRNA量を増加させることが示唆された。これらの結果から、骨格筋および脂肪組織に発言するいくつかのIGFBPは、栄養状態に応じて何らかの生理的役割を果たす可能性が示された。しかしながら、ニワトリ胚から調製した筋管細胞と前駆脂肪細胞の培養液への種々のPPARアゴニスト、グルコース、脂肪酸、或いはアミノ酸の添加は、IGFBP遺伝子のmRNA量に影響を及ぼさなかったことから、その調節因子は現在のところ不明である。


• 稲作を基盤とした耕・畜・産の連携による持続可能な食料生産への挑戦

  一般社団法人ヤンマー資源循環支援機構, 一般社団法人ヤンマー資源循環支援機構 2022年度助成事業, Apr. 2022 - Mar. 2023, Principal investigator


• 米作を基盤とした持続可能な我が国独自の鶏肉生産への挑戦

  本田 和久

  農畜産業振興機構, Aug. 2021 - Mar. 2022, Principal investigator


• 小豆7Sグロブリン由来脂肪吸収抑制ペプチドの同定

  本田 和久

  飯島藤十郎記念食品科学振興財団, 2020年度学術研究助成, Apr. 2021 - Mar. 2022, Principal investigator


• 飼料米によるひょうご味どりの高付加価値化の証明とその市場における評価の検証

  本田 和久

  兵庫県, アグリビジネス創出支援事業, 神戸大学, Aug. 2019 - Mar. 2021, Principal investigator


• 輸入トウモロコシ不使用の高付加価値鶏肉の生産による資源循環型農業の確立

  本田 和久

  一般社団法人ヤンマー資源循環支援機構, 2019年度助成事業, 神戸大学, Apr. 2019 - Mar. 2020, Principal investigator


• あずきに含まれる抗肥満因子の同定

  本田 和久

  財団法人日本豆類基金協会, 豆類振興事業助成金, 神戸大学, Apr. 2019 - Mar. 2020, Principal investigator


• 地鶏肉の美味しさの決定要因の解明

  本田 和久

  公益財団法人伊藤記念財団, 伊藤記念財団研究助成, 神戸大学, Jul. 2017 - Mar. 2020, Principal investigator


• Studies on chicken and sheep meat peptides which contribute to activation of brown adipose tissue and raise body temperature after consumption

  WAKAMATSU Jun-ichi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Hokkaido University, 01 Apr. 2016 - 31 Mar. 2019

  In in vivo study, the hydrophilic fraction of chicken artificial-digested peptide was suggested to have thermogenesis activity after consumption. Since there were some difference in meat digested- and absorbed-peptide among animal species. a small amount of animal species-specific peptide digested after consumption might have activity of thermogenesis. However, in vivo study using hepatocyte, the opposite results about gene expression were observed in vivo study. Therefore, it suggests that the digested and absorbed peptide of meat protein, especially chicken, do not increase the body temperature by acting on liver after absorption directly.


• 食料自給率の向上と持続的社会の構築に向けた国産鶏種と飼料米による純国産鶏肉の開発

  本田 和久

  農畜産業振興機構, 畜産関係学術研究委託調査, Aug. 2018 - Mar. 2019, Principal investigator


• 小豆に含まれる抗肥満因子の同定

  本田 和久

  飯島藤十郎記念食品科学振興財団, 2017年度学術研究助成, Apr. 2018 - Mar. 2019, Principal investigator


• 国産飼料の増産・鶏肉の輸出促進に向けた飼料用米による国産鶏肉の美味しさの向上

  本田 和久

  農畜産業振興機構, 畜産関係学術研究委託調査, Aug. 2017 - Mar. 2018, Principal investigator


• ひょうご味どりの効率的生産法の開発とその美味しさを生かした加工食品の開発

  本田 和久

  兵庫県, 「農」イノベーションひょうご研究開発プロジェクト支援事業, Jul. 2017 - Feb. 2018, Principal investigator

  Competitive research funding


• 亜鉛化合物を用いた鶏肉の高生産・肉質改善法の確立

  本田 和久

  科学研究費補助金／基盤研究(C), Apr. 2012 - Mar. 2015, Principal investigator

  Competitive research funding


• 食欲促進、抗ストレス及び抗肥満作用を有する新規ペプチドによる鶏肉の高生産法の開発

  本田 和久

  科学研究費補助金／基盤研究(C), 2009, Principal investigator

  Competitive research funding


• Examination of difference in central nervous system between chickens selected for meat and egg production

  SUGAHARA Kunio, FURUSE Mitsuhiro, SAITO Noboru, HIRAMATSU Koji, HONDA Kazuhisa

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Utsunomiya University, 2005 - 2007

  The aim of this project is to clarify the difference in the central nervous system related to homeostasis, feeding behavior, responses to stress and osmotic regulation, between chickens selected for meat and egg production. Neuropeptide Y (NPY) peptide content in the hypothalamus during embryonic and early post-hatched period was larger in the egg-type than in the meat-type chickens, which is inconsistent with orexigenic exogenous NPY. Compared to egg-type chickens, higher abundance proopiomelanocortin mRNA in the hypothalamus and larger anorexigenic effect of intracerebroventricularly injected beta-MSH in the meta-type chickens are likely to be responsible for their ability to eat more feed. In addition to this, the turnover rate of serotonin and dopamine in the hypothalamus might be also associated with the different feeding behavior between both types of chickens. Less locomotion activity and vocalization number induced by isolation of chickens was involved in the lower melatonin production in the pineal, lobe, brain stem in the meat-type chickens. Heat production during embryonic period was less and lipid metabolism in the meat-type chickens was more active than those in the egg-type chickens, which may be one of factors to make the former grow faster. Nagoya chickens being selected for meat- and egg-types were useful animals to show that the embryonic heat production and responses to stressor are items for selection. Arginine vasotocin gene expression in the hypothalamus responding to hypertonic saline injection was likely to be mediated through hypothalamic not only TonEBP but any other pathway than TonEBP. Distribution of cells and fibers immunoreactive to galanin and NPY in the hypothalamus was similar between both the types of chickens. NPY neuron in the hypothalamus was suggested to project to the parvocellular neuron of the paraventriclular nucleus in both types of chickens. Collectively, there is little differences in the structure of the neuron containing regulatory peptide between the two types of chickens, but some differences in the expression of peptide genes and in neurotransmitters metabolism have been shown to contribute to the phenotype of chickens.


• 食品素材を用いた「倹約遺伝子」発現制御による家禽の体脂肪蓄積抑制法の開発

  本田 和久

  科学研究費補助金／若手研究(B), 2005, Principal investigator

  Competitive research funding

• 家禽飼育方法、家禽飼育システムおよび鶏舎における照明管理システム

  特願2015-240349, 09 Dec. 2015, 特許6649763

  Patent right


• 血中トリグリセリド濃度上昇抑制ペプチド及び当該ペプチドを有効成分として含む血中トリグリセリド濃度上昇抑制剤

  特願平8-66916, 22 Mar. 1996, 特許2805194

  Patent right


• 血中トリグリセリド濃度上昇抑制ペプチド及び当該ペプチドを有効成分として含む血中トリグリセリド濃度上昇抑制剤

  特願平5-337885, 28 Dec. 1993, 特許2800877

  Patent right