Research activity information

• Nov. 2023 16th International Symposium on Nanomedicine (ISNM2023), Best Poster Awards, Model Membrane Arrays Prepared by Self-Spreading of Lipid Bilayers in Preformed Polymeric Lipid Bilayer Channels

  Masako FUJII

  International society


• Nov. 2023 第61回日本生物物理学会, 日本生物物理学会学生発表賞, 人工膜とナノ空間を用いた膜結合分子の動的挙動の計測

  長塚ななみ

  Japan society


• Jan. 2021 電気学会, 電気学会・技術委員会奨励賞, パターン化人工膜への膜タンパク質の再構成

  永井るりか

  Japan society


• May 2018 第9回日本光合成学会年会, 優秀研究賞, パターン化人工生体膜を用いた光合成機構の再構成

  米田 卓郎, 谷本 泰士, 高木 大輔, 森垣 憲一

  Japan society


• Oct. 2009 第82回日本生化学会大会, 優秀プレゼンテーション賞, ケージド化合物を用いたP450酵素活性の光制御

  水谷 和幸, 森垣 憲一, 達 吉郎, 一色 邦夫, 今石 浩正

  Japan society

• Supported Lipid Bilayer Arrays Formed from Inkjet-Printed Lipid Spots

  Yasushi Tanimoto, Misato Yamada, Masako Fujii, Keigo Kitayama, Fumio Hayashi, Chie Hosokawa, Kenichi Morigaki

  American Chemical Society (ACS), May 2025, Langmuir, 41(18) (18), 11284 - 11292

  [Refereed]

  Scientific journal


• Membrane tubulation induced by a bacterial glycolipid

  Kaoru Nomura, Akihiro Tsuji, Hayato Yamashita, Masayuki Abe, Kohki Fujikawa, Shoko Mori, Tsukiho Osawa, Hiromi Toyonaga, Tomohiro Osugi, Kazuma Yasuhara, Kenichi Morigaki, Ken-ichi Nishiyama, Keiko Shimamoto

  Springer Science and Business Media LLC, Mar. 2025, Scientific Reports, 15(1) (1)

  Scientific journal


• Evidence for a transfer-to-trap mechanism of fluorophore concentration quenching in lipid bilayers

  Sophie A. Meredith, Yuka Kusunoki, Stephen D. Evans, Kenichi Morigaki, Simon D. Connell, Peter G. Adams

  Elsevier BV, Sep. 2024, Biophysical Journal, 123(18) (18), 3242 - 3256, English

  [Refereed]

  Scientific journal


• Controlling Physical and Biochemical Parameters of Actin Nucleation Using a Patterned Model Lipid Membrane

  Yosuke Yamazaki, Yuuri Miyata, Kenichi Morigaki, Makito Miyazaki

  Corresponding, American Chemical Society (ACS), Jan. 2024, Nano Letters, 24(6) (6), 1825 - 1834

  [Refereed]

  Scientific journal


• Oligomerization and aggregation of NAP-22 with several metal ions

  Shohei Maekawa, Keisuke Yuzu, Eri Chatani, Kenichi Morigaki

  Elsevier BV, Jan. 2024, Neuroscience Letters, 821, 137623 - 137623, English

  [Refereed]

  Scientific journal


• Self-Quenching Behavior of a Fluorescent Probe Incorporated within Lipid Membranes Explored Using Electrophoresis and Fluorescence Lifetime Imaging Microscopy

  Sophie A. Meredith, Yuka Kusunoki, Simon D. Connell, Kenichi Morigaki, Stephen D. Evans, Peter G. Adams

  American Chemical Society (ACS), Feb. 2023, The Journal of Physical Chemistry B, 127(8) (8), 1715 - 1727, English

  [Refereed]

  Scientific journal


• Retarded Diffusion and Confinement of Membrane-Bound Molecules in a Patterned Hybrid Membrane of Phospholipid Bilayers and Monolayers.

  Yasushi Tanimoto, Yu Yoshimura, Fumio Hayashi, Kenichi Morigaki

  The biological membrane is a complex two-dimensional fluid, in which various molecular interactions regulate the lateral diffusion of membrane-associated molecules. Pinning of membrane proteins or lipids by extra-membrane proteins impedes the diffusion. In addition, coupling between two monolayer leaflets within a phospholipid bilayer via interdigitation plays important roles, though this effect remains elusive. Here, we fabricate a substrate-supported model membrane with patterned bilayer/monolayer regions to explore the influences of interleaflet coupling. A patterned monolayer of polymerized diacetylene phospholipid, 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DiynePC), was lithographically generated and used to form patterned lipid bilayers and monolayers. A phospholipid monolayer was formed on top of the polymerized monolayer. The bilayer/monolayer hybrid membrane was continuous and fluid, but lateral diffusion in the monolayer region was significantly retarded, suggesting the influences of interleaflet coupling. We reconstituted photoreceptor rhodopsin (Rh) and G-protein transducin (Gt) as model transmembrane and peripheral proteins. Rh diffused laterally only in the bilayer region, whereas Gt diffused in both bilayer and monolayer regions. The patterned hybrid bilayer/monolayer membrane reproduces the retarded diffusion and confinement of membrane-bound molecules in a controlled manner and provides insight into the physicochemical and functional roles of semipermeable corrals in the cell membrane.

  Jan. 2023, The journal of physical chemistry. B, 127(2) (2), 520 - 527, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Enhancing the spectral range of plant and bacterial light-harvesting pigment-protein complexes with various synthetic chromophores incorporated into lipid vesicles

  Ashley M. Hancock, David J.K. Swainsbury, Sophie A. Meredith, Kenichi Morigaki, C. Neil Hunter, Peter G. Adams

  Elsevier BV, Dec. 2022, Journal of Photochemistry and Photobiology B: Biology, 237, 112585 - 112585, English

  [Refereed]

  Scientific journal


• Nanofluidic Model Membrane for the Single-Molecule Observation of Membrane Proteins

  Ryota Komatsu, Yasushi Tanimoto, Koji Ando, Kazuma Yasuhara, Yasuhiko Iwasaki, Fumio Hayashi, Kenichi Morigaki

  Membrane proteins play essential roles in the cell, and they constitute one of the most important targets of drugs. Studying membrane proteins in a controlled model membrane environment can provide unambiguous, quantitative information on their molecular properties and functions. However, reconstituting membrane proteins in a model system poses formidable technological challenges. Here, we developed a novel model membrane platform for highly sensitive observation of membrane proteins by combining a micropatterned lipid membrane and a nanofluidic channel. A micropatterned model membrane was generated by lithographically integrating a polymerized lipid bilayer and a natural (fluid) lipid bilayer. A nanofluidic channel having a defined thickness was formed between the fluid bilayer and a polydimethylsiloxane (PDMS) slab by attaching the polymeric bilayer and PDMS slab using an adhesion layer composed of silica nanoparticles that are coated with a biocompatible polymer brush. As we reconstituted rhodopsin (Rh), a G-protein-coupled receptor (GPCR), from a detergent-solubilized state into the fluid bilayer, only successfully reconstituted Rh molecules diffused laterally in the lipid bilayer and migrated into the nanogap junction, where they could be observed with a vastly improved signal-to-background ratio. The nanogap junction effectively separates the sites of reconstitution and observation and provides a novel platform for studying the molecular properties and functions of membrane proteins at the single-molecular level.

  American Chemical Society (ACS), Jun. 2022, Langmuir, 38(23) (23), 7234 - 7243, English, International magazine

  [Refereed]

  Scientific journal


• Functional reconstitution of dopamine D2 receptor into a supported model membrane in a nanometric confinement

  Nagai, R, Sugimachi, A, Tanimoto, Y, Suzuki, K. G. N, Hayashi, F, Kasai, R. S, Morigaki, K

  Dopamine D2 receptor (D2R), a G-protein-coupled receptor (GPCR), plays critical roles in neural functions and represents the target for a wide variety of drugs used to treat neurological diseases. However, its fundamental physicochemical properties, such as dimerization and affinity to different lipid environments, remain unknown. Here, reconstitution and characterization of D2R in a supported model membrane in nanometric confinement are reported. D2R is expressed in Chinese hamster ovary (CHO) cells and transferred into the supported model membrane as cell membrane blebs. D2R molecules are reconstituted with an elevated density in the cleft between the substrate and poly(dimethylsiloxane) (PDMS) elastomer. Reconstituted D2R retains the physiological functions, as evaluated from its binding to an antagonist and dimerization lifetime. The transient dimer formation of D2R, similar to the live cell, suggests that it is an innate property that does not depend on the cellular structures such as actin filaments. Although the mechanism of this unique reconstitution process is currently not fully understood, the finding points to a new possibility of using a nanometric space (<100 nm thick) as a platform for reconstituting and studying membrane proteins under the quasi-physiological conditions, which are difficult to be created by other methods.

  Dec. 2021, Adv. Biology, 5(12) (12), 2100636, English, International magazine

  [Refereed]

  Scientific journal


• Reconstitution of Membrane Proteins into a Model Biological Membrane

  Nagai Rurika, Sugimachi Ayane, Tanimoto Yasushi, G. N. Suzuki Kenichi, Hayashi Fumio, S. Kasai Rinshi, Morigaki Kenichi

  Model biological membranes are a useful tool to study the molecular properties and functions of membrane proteins. We develop a strategy to directly reconstitute mammalian membrane proteins from the cell membrane into a model biological membrane to bypass the technically challenging solubilization and purification processes. We expressed dopamine D2 receptor (D2R), a G-protein coupled receptor (GPCR), in Chinese hamster ovary (CHO) cells and produced cell membrane blebs by chemical induction. By introducing blebs into a patterned framework of lithographically-polymerized lipid bilayer on the substrate surface, we could form a planar bilayer and observe single molecules of D2R. Interestingly, a much higher density of D2R molecules were reconstituted in a nanometric cleft between the substrate and a poly-dimethylsiloxane (PDMS) elastomer sheet. This methodology should enable to evaluate the physicochemical properties and functions of a wide range of mammalian membrane proteins.

  The Institute of Electrical Engineers of Japan, Dec. 2021, IEEJ Transactions on Electronics, Information and Systems, 141(12) (12), 1340 - 1343, Japanese

  [Refereed][Invited]

  Scientific journal


• Model Lipid Membranes Assembled from Natural Plant Thylakoids into 2D Microarray Patterns as a Platform to Assess the Organization and Photophysics of Light‐Harvesting Proteins

  Sophie A. Meredith, Takuro Yoneda, Ashley M. Hancock, Simon D. Connell, Stephen D. Evans, Kenichi Morigaki, Peter G. Adams

  Wiley, Apr. 2021, Small, 17(14) (14), 2006608 - 2006608, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• New lipid membrane technologies for reconstitution, analysis, and utilization of ‘living’ membrane proteins

  Kazuma Yasuhara, Kenichi Morigaki

  Biophysical Society of Japan, Sep. 2020, Biophysics and Physicobiology, 17(0) (0), 125 - 129, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The effects of phospholipids and fatty acids on the oligomer formation of NAP-22

  Sin-Ichi Odagaki, Shohei Maekawa, Fumio Hayashi, Toshinobu Suzaki, Kenichi Morigaki

  Last, Elsevier BV, Sep. 2020, Neuroscience Letters, 736, 135288 - 135288, English

  [Refereed]

  Scientific journal


• Photosynthetic Model Membranes of Natural Plant Thylakoid Embedded in a Patterned Polymeric Lipid Bilayer

  Takuro Yoneda, Yasushi Tanimoto, Daisuke Takagi, Kenichi Morigaki

  Thylakoid membranes in the chloroplast of plants, algae, and cyanobacteria are the powerhouse of photosynthesis, capturing solar energy and converting it into chemical energy. Although their structures and functions have been extensively studied, the intrinsically heterogeneous and dynamic nature of the membrane structures is still not fully understood. Investigating native thylakoid membranes in vivo is difficult due to their small size and limited external access to the chloroplast interior, while the bottom-up approaches based on model systems have been hampered by the sheer complexity of the native membrane. Here, we try to fill the gap by reconstituting the whole thylakoid membrane into a patterned substrate-supported planer bilayer. A mixture of thylakoid membrane purified from spinach leaves and synthetic phospholipid 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) vesicles spontaneously formed a laterally continuous and fluid two-dimensional (2D) membrane in the scaffold of the patterned polymeric bilayer. Chlorophyll fluorescence arising from photosystem II (PSII) recovered after photobleaching, suggesting that the membrane components are laterally mobile. The reversible changes of chlorophyll fluorescence in the presence of the electron acceptors and/or inhibitors indicated that the electron transfer activity of PSII was retained. Furthermore, we confirmed the electron transfer activity of photosystem I (PSI) by observing the generation of nicotinamide adenine dinucleotide phosphate (NADPH) in the presence of water-soluble ferredoxin and ferredoxin-NADP+ reductase. The lateral mobility of membrane-bound molecules and the functional reconstitution of major photosystems provide evidence that our hybrid thylakoid membranes could be an excellent experimental platform to study the 2D molecular organization and machinery of photosynthesis.

  Last, American Chemical Society (ACS), Jun. 2020, Langmuir, 36(21) (21), 5863 - 5871, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Affinity of rhodopsin to raft enables the aligned oligomer formation from dimers: Coarse-grained molecular dynamics simulation of disk membranes

  Yukito Kaneshige, Fumio Hayashi, Kenichi Morigaki, Yasushi Tanimoto, Hayato Yamashita, Masashi Fujii, Akinori Awazu

  The visual photopigment protein rhodopsin (Rh) is a typical G protein-coupled receptor (GPCR) that initiates the phototransduction cascade in retinal disk membrane of rod-photoreceptor cells. Rh molecule has a tendency to form dimer, and the dimer tends to form rows, which is suggested to heighten phototransduction efficiency in single-photon regime. In addition, the dimerization confers Rh an affinity for lipid raft, i.e. raftophilicity. However, the mechanism by which Rh-dimer raftophilicity contributes to the organization of the higher order structure remains unknown. In this study, we performed coarse-grained molecular dynamics simulations of a disk membrane model containing unsaturated lipids, saturated lipids with cholesterol, and Rh-dimers. We described the Rh-dimers by two-dimensional particle populations where the palmitoyl moieties of each Rh exhibits raftophilicity. We simulated the structuring of Rh in a disk for two types of Rh-dimer, i.e., the most and second most stable Rh dimers, which exposes the raftophilic regions at the dimerization-interface (H1/H8 dimer) and two edges away from the interface (H4/H5 dimer), respectively. Our simulations revealed that only the H1/H8 dimer could form a row structure. A small number of raftophilic lipids recruited to and intercalated in a narrow space between H1/H8 dimers stabilize the side-by-side interaction between dimers in a row. Our results implicate that the nano-sized lipid raft domains act as a "glue" to organize the long row structures of Rh-dimers.

  Public Library of Science (PLoS), Feb. 2020, PLOS ONE, 15(2) (2), e0226123 - e0226123, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Self-Spreading of Phospholipid Bilayer in a Patterned Framework of Polymeric Bilayer

  Fuyuko Tamura, Yasushi Tanimoto, Rurika Nagai, Fumio Hayashi, Kenichi Morigaki

  Phospholipid bilayers spontaneously spread on a hydrophilic substrate such as glass in aqueous solution due to the energetic gain of surface wetting. This process (self-spreading) was utilized to form a patterned model biological membrane containing reconstituted membrane proteins. A mechanically stable framework of a polymerized lipid bilayer was first generated by the lithographic polymerization of a diacetylene phospholipid. Then, natural lipid membranes (fluid bilayers) were introduced into the channels between polymeric bilayers by the self-spreading from a phospholipid reservoir. The spreading velocity could be fitted into a slope of -0.5 in a double logarithmic plot versus time due to the balance between the spreading force and resistive drag. The preformed polymeric bilayer accelerated the spreading by the energetic gain of covering hydrophobic edges with a lipid bilayer. At the same time, the domains of the polymeric bilayer obstructed spreading, and the spreading velocity linearly decreased with their fractional coverage. Above the critical coverage of ca. 50%, self-spreading was completely blocked (percolation threshold) and the fluid bilayer was confined in the polymer-free regions. Nonspecific adsorption of lipids onto the surface of polymeric bilayers was negligible, which enabled a heightened signal-to-background ratio in the reconstitution and observation of membrane proteins. Self-spread bilayers had a higher density of lipids than those formed by the spontaneous rupture of vesicles (vesicle fusion), presumably due to the continual supply of lipid molecules from the reservoir. These features give the self-spreading important advantages for preparing patterned model membranes with reconstituted membrane proteins.

  American Chemical Society (ACS), Nov. 2019, Langmuir, 35(45) (45), 14696 - 14703, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Raftophilic rhodopsin clusters offer stochastic platforms for G protein signalling in retinal discs

  Hayashi F, Saito N, Tanimoto Y, Okada K, Morigaki K, Seno K, Maekawa S

  Rhodopsin is a G protein-coupled receptor (GPCR) that initiates the phototransduction cascade in retinal disc membrane. Recent studies have suggested that rhodopsin forms highly ordered rows of dimers responsible for single-photon detection by rod photoreceptors. Dimerization is also known to confer to rhodopsin a high affinity for ordered lipids (raftophilicity). However, the role of rhodopsin organization and its raftophilicity in phototransduction remains obscure, owing to the lack of direct observation of rhodopsin dynamics and distribution in native discs. Here, we explore the single-molecule and semi-multimolecule behaviour of rhodopsin in native discs. Rhodopsin forms transient meso-scale clusters, even in darkness, which are loosely confined to the disc centre. Cognate G protein transducin co-distributes with rhodopsin, and exhibits lateral translocation to the disc periphery upon activation. We demonstrate that rhodopsin offers inherently distributed and stochastic platforms for G protein signalling by self-organizing raftophilic clusters, which continually repeat generation/extinction in the disc membrane.

  2019, Communications Biology, In press, 209 - 209, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Alteration of membrane physicochemical properties by two factors for membrane protein integration

  Nomura K, Yamaguchi T, Mori S, Fujikawa K, Nishiyama K, Shimanouchi T, Tanimoto Y, Morigaki K, Shimamoto K

  Elsevier BV, 2019, Biophys. J., In press(1) (1), 99 - 110, English

  [Refereed]

  Scientific journal


• Nanofluidic biosensor created by bonding patterned model cell membrane and silicone elastomer with silica nanoparticles

  Tanabe M, Ando K, Komatsu R, Morigaki K

  Oct. 2018, Small, 14(49) (49), 1802804, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs

  Kinoshita M, Suzuki K, Matsumori N, Takada M, Ano H, Morigaki K, Abe M, Makino A, Kobayashi T, Hirosawa K, Fujiwara T, Kusumi A, Murata M

  脂質の挙動をありのままに再現する蛍光プローブでラフトの形成機構を解明. 京都大学プレスリリース. 2017-03-28.Sphingomyelin (SM) has been proposed to form cholesterol-dependent raft domains and sphingolipid domains in the plasma membrane (PM). How SM contributes to the formation and function of these domains remains unknown, primarily because of the scarcity of suitable fluorescent SM analogs. We developed new fluorescent SM analogs by conjugating a hydrophilic fluorophore to the SM choline headgroup without eliminating its positive charge, via a hydrophilic nonaethylene glycol linker. The new analogs behaved similarly to the native SM in terms of their partitioning behaviors in artificial liquid order-disorder phase-separated membranes and detergent-resistant PM preparations. Single fluorescent molecule tracking in the live-cell PM revealed that they indirectly interact with each other in cholesterol- and sphingosine backbone{textendash}dependent manners, and that, for ~{}10{textendash}50 ms, they undergo transient colocalization-codiffusion with a glycosylphosphatidylinositol (GPI)-anchored protein, CD59 (in monomers, transient-dimer rafts, and clusters), in CD59-oligomer size{textendash}, cholesterol-, and GPI anchoring{textendash}dependent manners. These results suggest that SM continually and rapidly exchanges between CD59-associated raft domains and the bulk PM.

  Rockefeller University Press, Mar. 2017, J. Cell Biol., 168, 1183 - 1204, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Hybrid model membrane combining micropatterned lipid bilayer and hydrophilic polymer brush

  Morigaki, Kenichi, Nishimura, Toshiki, Tamura, Fuyuko, Tanimoto, Yasushi, Ando, Koji, Sudo, Yuki, Hayashi, Fumio, Iwasaki, Yasuhiko

  2017, Abstracts of Papers of the American Chemical Society, 253

  [Refereed]

  Scientific journal


• Hybrid Model Membrane Combining Micropatterned Lipid Bilayer and Hydrophilic Polymer Brush

  Nishimura, Toshiki, Tamura, Fuyuko, Kobayashi, Sawako, Tanimoto, Yasushi, Hayashi, Fumio, Sudo, Yuki, Iwasaki, Yasuhiko, Morigaki, Kenichi

  2017, Langmuir, 33(23) (23), 5752 - 5759, English

  [Refereed]

  Scientific journal


• The Role of the Prod1 Membrane Anchor in Newt Limb Regeneration

  Kaoru Nomura, Yasushi Tanimoto, Fumio Hayashi, Erisa Harada, Xiao-Yuan Shan, Masafumi Shionyu, Atsushi Hijikata, Tsuyoshi Shirai, Kenichi Morigaki, Keiko Shimamoto

  Jan. 2017, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 56(1) (1), 270 - 274, English

  [Refereed]

  Scientific journal


• Nanometric Gap Structure with a Fluid Lipid Bilayer for the Selective Transport and Detection of Biological Molecules

  Koji Ando, Masashi Tanabe, Kenichi Morigaki

  Aug. 2016, LANGMUIR, 32(31) (31), 7958 - 7964, English

  [Refereed]

  Scientific journal


• Evaluating the Raftophilicity of Rhodopsin Photoreceptor in a Patterned Model Membrane

  Yasushi Tanimoto, Keisuke Okada, Fumio Hayashi, Kenichi Morigaki

  Dec. 2015, BIOPHYSICAL JOURNAL, 109(11) (11), 2307 - 2316, English

  [Refereed]

  Scientific journal


• Uptake of raft components into amyloid beta-peptide aggregates and membrane damage

  Kenji Sasahara, Kenichi Morigaki, Yasuko Mori

  Jul. 2015, ANALYTICAL BIOCHEMISTRY, 481(1) (1), 18 - 26, English

  [Refereed]

  Scientific journal


• Model biological membrane combining patterned lipid bilayer and nanometric gap structure

  Morigaki Kenichi, Ando Koji, Tanabe Masashi

  生物は多様な夾雑分子が存在する環境でも標的分子を超高感度で検出できる。この能力には、生体膜の特性（流動性、非特異的吸着の抑制）が重要な役割を果たしている。我々は、高濃度に夾雑分子が存在する溶液から標的分子のみを選択的かつ高感度に計測できる技術を創出するため、ガラス基板表面にポリマー脂質膜と流動性脂質膜をパターン化形成し、厚さ数十ナノメートルのナノ空間を組み合わせた新規人工生体膜を開発した。

  The Surface Science Society of Japan, 2015, Abstract of annual meeting of the Surface Science of Japan, 35, 22 - 22


• Poly(dimethylsiloxane) (PDMS) surface patterning by biocompatible photo-crosslinking block copolymers

  Kuroda, Keita, Miyoshi, Hiromi, Fujii, Shota, Hirai, Tomoyasu, Takahara, Atsushi, Nakao, Aiko, Iwasaki, Yasuhiko, Morigaki, Kenichi, Ishihara, Kazuhiko, Yusa, Shin-ichi

  2015, Rsc Advances, 5(58) (58), 46686 - 46693, English

  [Refereed]

  Scientific journal


• Micropatterned model membrane with quantitatively controlled separation of lipid phases

  Fumiko Okada, Kenichi Morigaki

  2015, RSC ADVANCES, 5(2) (2), 1507 - 1513, English

  [Refereed]

  Scientific journal


• Amyloid aggregation and deposition of human islet amyloid polypeptide at membrane interfaces

  Kenji Sasahara, Kenichi Morigaki, Kyoko Shinya

  Jun. 2014, FEBS JOURNAL, 281(11) (11), 2597 - 2612, English

  [Refereed]

  Scientific journal


• Preparation of calcium carbonate microparticles containing organic fluorescent molecules from vaterite

  Masahiro Fujiwara, Kumi Shiokawa, Takayuki Kubota, Kenichi Morigaki

  May 2014, ADVANCED POWDER TECHNOLOGY, 25(3) (3), 1147 - 1154, English

  [Refereed]

  Scientific journal


• 1SEA-05 Micropatterned model membrane for studying the affinity of proteins to lipid raft(1SEA What do we know about lipid rafts?: New landscape at the frontier,Symposium,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

  Morigaki Kenichi, Tanimoto Yasushi, Okada Fumiko, Hayashi Fumio

  The Biophysical Society of Japan General Incorporated Association, 2014, Seibutsu Butsuri, 54(1) (1), S119, English


• 2P205 Hybrid model membrane composed of phospholipid bilayer and polymeric materials(13A. Biological & Artificial membrane:Structure & Property,Poster)

  Ando Koji, Morigaki Kenichi

  The Biophysical Society of Japan General Incorporated Association, 2014, Seibutsu Butsuri, 54(1) (1), S229, English


• 2P225 Evaluating the raftophilicity of rhodopsin in a patterned model membrane(13E. Biological & Artificial membrane:Signal transduction,Poster)

  Tanimoto Yasushi, Morigaki Kenichi, Hayashi Humio

  The Biophysical Society of Japan General Incorporated Association, 2014, Seibutsu Butsuri, 54(1) (1), S232, English


• Parallel assay of inkjet-printed cytochrome P450

  Emi Kanemura, Tatsushi Goto, Yoshiro Tatsu, Hiromasa Imaishi, Kenichi Morigaki

  2014, ANALYTICAL METHODS, 6(7) (7), 2117 - 2124, English

  [Refereed]

  Scientific journal


• Microarrays of phospholipid bilayers generated by inkjet printing

  Misato Yamada, Hiromasa Imaishi, Kenichi Morigaki

  May 2013, Langmuir, 29(21) (21), 6404 - 6408, English

  [Refereed]

  Scientific journal


• Surface Functionalization of a Polymeric Lipid Bilayer for Coupling a Model Biological Membrane with Molecules, Cells, and Microstructures

  Kenichi Morigaki, Kazuyuki Mizutani, Makoto Saito, Takashi Okazaki, Yoshihiro Nakajima, Yoshiro Tatsu, Hiromasa Imaishi

  Feb. 2013, LANGMUIR, 29(8) (8), 2722 - 2730, English

  [Refereed]

  Scientific journal


• 1P207 Reconstitution of rhodopsin into a micropatterned model biological membrane(13A.Biological & Artifical membrane: Structure & Property,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)

  Tanimoto Yasushi, Morigaki Kenichi, Hayashi Fumio

  The Biophysical Society of Japan General Incorporated Association, 2013, Seibutsu Butsuri, 53(1) (1), S140, English


• Effects of membrane interaction and aggregation of amyloid beta-peptide on lipid mobility and membrane domain structure

  Kenji Sasahara, Kenichi Morigaki, Kyoko Shinya

  2013, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 15(23) (23), 8929 - 8939, English

  [Refereed]

  Scientific journal


• Localized surface plasmon microscopy of submicron domain structures of mixed lipid bilayers

  Koyo Watanabe, Ryosuke Miyazaki, Goro Terakado, Takashi Okazaki, Kenichi Morigaki, Hiroshi Kano

  Sep. 2012, BIOMEDICAL OPTICS EXPRESS, 3(9) (9), 2012 - 2020, English

  [Refereed]

  Scientific journal


• Binding of Islet Amyloid Polypeptide to Supported Lipid Bilayers and Amyloid Aggregation at the Membranes

  Kenji Sasahara, Kenichi Morigaki, Takashi Okazaki, Daizo Hamada

  Sep. 2012, BIOCHEMISTRY, 51(35) (35), 6908 - 6919, English

  [Refereed]

  Scientific journal


• Microarray of Human P450 with an Integrated Oxygen Sensing Film for High-Throughput Detection of Metabolic Activities

  Gang Chang, Yoshinao Mori, Saori Mori, Takashi Irie, Hidenori Nagai, Tatsushi Goto, Yoshiro Tatsu, Hiromasa Imaishi, Kenichi Morigaki

  Jun. 2012, ANALYTICAL CHEMISTRY, 84(12) (12), 5292 - 5297, English

  [Refereed]

  Scientific journal


• Formation of Substrate-Supported Membranes from Mixtures of Long- and Short-Chain Phospholipids

  Kenichi Morigaki, Shigeki Kimura, Keisuke Okada, Takashi Kawasaki, Kazunori Kawasaki

  Jun. 2012, LANGMUIR, 28(25) (25), 9649 - 9655, English

  [Refereed]

  Scientific journal


• Surface functionalization of polymeric lipid bilayers for coupling a model membrane with molecules, cells, and 3D objects

  Kenichi Morigaki, Makoto Saito, Kazuyuki Mizutani, Takashi Okazaki, Yoshihiro Nakajima, Yoshiro Tatsu, Hiromasa Imaishi

  Mar. 2012, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 243, English

  [Refereed]


• 3PT161 Reconstitution of rhodopsin into micropatterned model biological membrane(The 50th Annual Meeting of the Biophysical Society of Japan)

  Okada Keisuke, Morigaki Kenichi, Hayashi Fumio

  The Biophysical Society of Japan General Incorporated Association, 2012, Seibutsu Butsuri, 52, S168 - S169, English


• Photoregulation of Cytochrome P450 Activity by Using Caged Compound

  Kenichi Morigaki, Kazuyuki Mizutani, Emi Kanemura, Yoshiro Tatsu, Noboru Yumoto, Hiromasa Imaishi

  Jan. 2012, ANALYTICAL CHEMISTRY, 84(1) (1), 155 - 160, English

  [Refereed]

  Scientific journal


• Hydration and Temperature Dependence of C-13 and H-1 NMR Spectra of the DMPC Phospholipid Membrane and Complete Resonance Assignment of Its Crystalline State

  Kaoru Nomura, Masami Lintuluoto, Kenichi Morigaki

  Dec. 2011, JOURNAL OF PHYSICAL CHEMISTRY B, 115(50) (50), 14991 - 15001, English

  [Refereed]

  Scientific journal


• Fusion of Lipid Vesicles with Planar Lipid Bilayers Induced by a Combination of Peptides

  Takehiko Inaba, Yoshiro Tatsu, Kenichi Morigaki

  Oct. 2011, LANGMUIR, 27(20) (20), 12515 - 12520, English

  [Refereed]

  Scientific journal


• Sensitive bioimaging in microfluidic channels on the plasmonic substrate: Application of an enhanced fluorescence based on the reverse coupling mode

  Keiko Tawa, Xiaoqiang Cui, Kenji Kintaka, Junji Nishii, Kenichi Morigaki

  Jun. 2011, JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 221(2-3) (2-3), 261 - 267, English

  [Refereed]

  Scientific journal


• Vertically Integrated Human P450 and Oxygen Sensing Film for the Assays of P450 Metabolic Activities

  Gang Chang, Kenichi Morigaki, Yoshiro Tatsu, Takashi Hikawa, Tatsushi Goto, Hiromasa Imaishi

  Apr. 2011, ANALYTICAL CHEMISTRY, 83(8) (8), 2956 - 2963, English

  [Refereed]

  Scientific journal


• The Effects of Single Nucleotide Polymorphisms in CYP2A13 on Metabolism of 5-Methoxypsoralen

  Tatsushi Goto, Hiroshi Moriuchi, Xuejun Fu, Tomoyo Ikegawa, Toshiyuki Matsubara, Gang Chang, Tomohide Uno, Kenichi Morigaki, Kunio Isshiki, Hiromasa Imaishi

  Dec. 2010, DRUG METABOLISM AND DISPOSITION, 38(12) (12), 2110 - 2116, English

  [Refereed]

  Scientific journal


• In situ imaging of micropatterned phospholipid membranes by surface plasmon fluorescence microscopy

  Keiko Tawa, Kenichi Morigaki

  Dec. 2010, COLLOIDS AND SURFACES B-BIOINTERFACES, 81(2) (2), 447 - 451, English

  [Refereed]

  Scientific journal


• Glucose concentration determination based on silica sol-gel encapsulated glucose oxidase optical biosensor arrays

  Gang Chang, Yoshiro Tatsu, Tatsushi Goto, Hiromasa Imaishi, Kenichi Morigaki

  Nov. 2010, TALANTA, 83(1) (1), 61 - 65, English

  [Refereed]

  Scientific journal


• Encapsulation of Proteins into CaCO3 by Phase Transition from Vaterite to Calcite

  Masahiro Fujiwara, Kumi Shiokawa, Miyuki Araki, Nobuyuki Ashitaka, Kenichi Morigaki, Takayuki Kubota, Yoshiko Nakahara

  Sep. 2010, CRYSTAL GROWTH & DESIGN, 10(9) (9), 4030 - 4037, English

  [Refereed]

  Scientific journal


• Phase Separation of Lipid Microdomains Controlled by Polymerized Lipid Bilayer Matrices

  Takashi Okazaki, Yoshiro Tatsu, Kenichi Morigaki

  Mar. 2010, LANGMUIR, 26(6) (6), 4126 - 4129, English

  [Refereed]

  Scientific journal


• High resolution imaging of patterned model biological membranes by localized surface plasmon microscopy

  Koyo Watanabe, Miyazaki Ryosuke, Goro Terakado, Takashi Okazaki, Kenichi Morigaki, Hiroshi Kano

  Feb. 2010, APPLIED OPTICS, 49(5) (5), 887 - 891, English

  [Refereed]

  Scientific journal


• Specific protein binding on phospholipid bilayer array corralled by nonfouling polymer brushes

  Iwasaki, Yasuhiko, Nakai, Kosuke, Morigaki, Kenichi

  2010, Abstracts of Papers of the American Chemical Society, 240

  [Refereed]

  Scientific journal


• 食品の安全性評価へのバイオセンサーの利用

  今石 浩正, 後藤 達志, 宇野 知秀, 森垣 憲一

  2010, BRAIN TECHNO NEWS, 142, 21-25, Japanese

  [Refereed]

  Scientific journal


• Molecular recognition on fluidic lipid bilayer microarray corralled by well-defined polymer brushes

  Nakai, K, Morigaki, K, Iwasaki, Y

  2010, Soft Matter, 6(23) (23), 5937 - 5943, English

  [Refereed]

  Scientific journal


• 3P-180 Detergent assisted formation of substrate supported phospholipid membranes(Biol & Artifi memb.:Structure & Property,Oral Presentations,The 47th Annual Meeting of the Biophysical Society of Japan)

  Morigaki Kenichi, Kimura Shigeki, Inaba Takehiko, Okazaki Takashi, Kawasaki Takashi, Imaishi Hiromasa

  The Biophysical Society of Japan General Incorporated Association, 2009, Seibutsu Butsuri, 49, S181, English


• Polymerized Lipid Bilayers on a Solid Substrate: Morphologies and Obstruction of Lateral Diffusion

  Takashi Okazaki, Takehiko Inaba, Yoshiro Tatsu, Ryugo Tero, Tsuneo Urisu, Kenichi Morigaki

  Jan. 2009, LANGMUIR, 25(1) (1), 345 - 351, English

  [Refereed]

  Scientific journal


• Interaction of lipopolysaccharide and phospholipid in mixed membranes: Solid-state P-31-NMR spectroscopic and microscopic investigations

  Kaoru Nomura, Takehiko Inaba, Kenichi Morigaki, Klaus Brandenburg, Ulrich Seydel, Shoichi Kusumoto

  Aug. 2008, BIOPHYSICAL JOURNAL, 95(3) (3), 1226 - 1238, English

  [Refereed]

  Scientific journal


• Biotin-containing phospholipid vesicle layer formed on self-assembled monolayer of a saccharide-terminated alkyl disulfide for surface plasmon resonance biosensing

  Yoshiko Ishizuka-Katsura, Tetsuichi Wazawa, Tadato Ban, Kenichi Morigaki, Shigeru Aoyama

  May 2008, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 105(5) (5), 527 - 535, English

  [Refereed]

  Scientific journal


• Calcium phosphate composite materials including inorganic powders, BSA or duplex DNA prepared by W/O/W interfacial reaction method

  Masahiro Fujiwara, Kurni Shiokawa, Kenichi Morigaki, Yoshiro Tatsu, Yoshiko Nakahara

  Mar. 2008, MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, 28(2) (2), 280 - 288, English

  [Refereed]

  Scientific journal


• Visualization and classification of amyloid beta supramolecular assemblies

  Hisashi Yagi, Tadato Ban, Kenichi Morigaki, Hironobu Naiki, Yuji Goto

  Dec. 2007, BIOCHEMISTRY, 46(51) (51), 15009 - 15017, English

  [Refereed]

  Scientific journal


• Polymerization of diacetylene phospholipid Bilayers on solid substrate: Influence of the film deposition temperature

  Kenichi Morigaki, Holger Schoenherr, Takashi Okazaki

  Nov. 2007, LANGMUIR, 23(24) (24), 12254 - 12260, English

  [Refereed]

  Scientific journal


• Immobilization and activity assay of cytochrome P450 on patterned lipid membranes

  Yoshihiro Ueda, Kenichi Morigaki, Yoshiro Tatsu, Noboru Yumoto, Hiromasa Imaishi

  Apr. 2007, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 355(4) (4), 926 - 931, English

  [Refereed]

  Scientific journal


• Direct encapsulation of BSA and DNA into silica microcapsules (hollow spheres)

  Masahiro Fujiwara, Kumi Shiokawa, Kaoru Hayashi, Kenichi Morigaki, Yoshiko Nakahara

  Apr. 2007, JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 81A(1) (1), 103 - 112, English

  [Refereed]

  Scientific journal


• 2P261 Composite membranes of fluid and polymerized phospholipid bilayers on solid substrate(Native and artificial biomembranes-structure and properties,Poster Presentations)

  Okazaki Takashi, Inaba Takehiko, Tatsu Yoshiro, Morigaki Kenichi

  The Biophysical Society of Japan General Incorporated Association, 2007, Seibutsu Butsuri, 47, S178, English


• 2P259 Immobilization of cytochrome P450 on micropatterned lipid bilayer substrates for the parallel assay of their activities(Native and artificial biomembranes-structure and properties,Poster Presentations)

  Ueda Yoshihiro, Morigaki Kenichi, Tatsu Yoshiro, Yumoto Noboru, Imaishi Hiromasa

  The Biophysical Society of Japan General Incorporated Association, 2007, Seibutsu Butsuri, 47, S177, English


• 2P260 The effect of membrane fusion peptides on interactions between liposomes and supported planar lipid bilayers : TIRF observations(Native and artificial biomembranes-structure and properties,Poster Presentations)

  INABA Takehiko, TATSU Yoshiro, MORIGAKI Kenichi

  The Biophysical Society of Japan General Incorporated Association, 2007, Seibutsu Butsuri, 47, S178, English


• Effects of fusogenic peptides on substrate supported planar lipid bilayers.

  Takehiko Inaba, Kenichi Morigaki, Yoshiro Tatsu

  Jan. 2007, BIOPHYSICAL JOURNAL, 577A - 577A, English

  [Refereed]


• Vesicles from docosahexaenoic acid

  Trishool Namani, Takashi Ishikawa, Kenichi Morigaki, Peter Walde

  Jan. 2007, COLLOIDS AND SURFACES B-BIOINTERFACES, 54(1) (1), 118 - 123, English

  [Refereed]

  Scientific journal


• Real-time and single fibril observation of the formation of amyloid beta spherulitic structures

  Tadato Ban, Kenichi Morigaki, Hisashi Yagi, Takashi Kawasaki, Atsuko Kobayashi, Shunsuke Yuba, Hironobu Naiki, Yuji Goto

  Nov. 2006, JOURNAL OF BIOLOGICAL CHEMISTRY, 281(44) (44), 33677 - 33683, English

  [Refereed]

  Scientific journal


• 2P076 Effects of surface interaction on the Aβ(1-40) amyloid fibril formation and growth

  Ban T., Morigaki K., Naiki H., Goto Y.

  The Biophysical Society of Japan General Incorporated Association, 2005, Seibutsu Butsuri, 45, S138, Japanese


• Micropatterned composite membranes of polymerized and fluid lipid bilayers : Incorporation of fluid bilayers by the vesicle fusion process

  Okazaki T., Morigaki K.

  The Biophysical Society of Japan General Incorporated Association, 2005, Seibutsu Butsuri, 45, S263, Japanese


• Substrate supported phospholipid membranes studied by SPR and surface plasmon fluorescence spectroscopy (SPFS)

  Morigaki K., Tawa K.

  The Biophysical Society of Japan General Incorporated Association, 2005, Seibutsu Butsuri, 45, S263, Japanese


• 1P212 Lithographic micro-patterning of substrate-supported lipid bilayers

  Morigaki K.

  The Biophysical Society of Japan General Incorporated Association, 2004, Seibutsu Butsuri, 44, S82, Japanese


• 1G0900 Patterning solid-supported lipid bilayer membranes by lithgraphic polymerization of a diacetylene lipid

  Morigaki K.

  The Biophysical Society of Japan General Incorporated Association, 2002, Seibutsu Butsuri, 42(2) (2), S35, Japanese

• Integrated model membrane for biophysical studies and biomedical applications

  Kenichi Morigaki

  Abstract The biological membrane is a dynamic supramolecular architecture that plays vital roles in the cell. However, understanding the physicochemical properties and functions of the membrane supramolecular system is difficult. We have developed an integrated model system of the biological membrane comprising patterned polymeric and natural lipid bilayers. The polymeric bilayer acts as a framework to support embedded natural membranes. The embedded natural membranes retain important characteristics of the biological membrane such as fluidity, and reproduces the physical states and functions of the biological membrane. Membrane proteins can be reconstituted into the model membrane for analyzing their functions in a controlled lipid membrane environment. Three-dimensional structures can be constructed by attaching micro-/nano-fabricated structures to the polymeric bilayer framework. The integrated model membrane realizes a versatile platform to study membrane functions, and should open new opportunities in fundamental biological sciences as well as biomedical/analytical applications.

  Corresponding, IOP Publishing, 01 Apr. 2024, Japanese Journal of Applied Physics, 63(4) (4), 040801 - 040801, English

  [Refereed][Invited]

  Introduction scientific journal


• 光重合性リン脂質と天然リン脂質を組み合わせたパターン化人工膜による生体膜機能の再構成と応用

  森垣憲一

  Lead, Feb. 2021, 膜, 46, 65 - 40, Japanese

  [Refereed][Invited]

  Introduction scientific journal


• Substrate-supported model membrane as a versatile analytical/ biosensing platform

  Kenichi Morigaki

  Lead, Jan. 2021, Analytical Sciences, 37, English

  [Refereed][Invited]

  Introduction scientific journal

  Link to Kobe Univ. Repository (Kernel)


• パターン化人工膜を用いた生体膜機能の解析

  森垣憲一

  Lead, Jul. 2019, 生物物理, 59(4) (4), 188 - 191, Japanese

  [Refereed][Invited]

  Introduction scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Evolution and development of model membranes for physicochemical and functional studies of the membrane lateral heterogeneity”, Biochim. Biophys. Acta - Biomembranes

  Morigaki K, Tanimoto Y

  One of the main questions in the membrane biology is the functional roles of membrane heterogeneity and molecular localization. Although segregation and local enrichment of protein/lipid components (rafts) have been extensively studied, the presence and functions of such membrane domains still remain elusive. Along with biochemical, cell observation, and simulation studies, model membranes are emerging as an important tool for understanding the biological membrane, providing quantitative information on the physicochemical properties of membrane proteins and lipids. Segregation of fluid lipid bilayer into liquid-ordered (Lo) and liquid-disordered (Ld) phases has been studied as a simplified model of raft in model membranes, including giant unilamellar vesicles (GUVs), giant plasma membrane vesicles (GPMVs), and supported lipid bilayers (SLB). Partition coefficients of membrane proteins between Lo and Ld phases were measured to gauze their affinities to lipid rafts (raftophilicity). One important development in model membrane is patterned SLB based on the microfabrication technology. Patterned Lo/Ld phases have been applied to study the partition and function of membrane-bound molecules. Quantitative information of individual molecular species attained by model membranes is critical for elucidating the molecular functions in the complex web of molecular interactions. The present review gives a short account of the model membranes developed for studying the lateral heterogeneity, especially focusing on patterned model membranes on solid substrates.

  Oct. 2018, Biochim. Biophys. Acta - Biomembranes, 1860(10) (10), 2012 - 2017, English, International magazine

  [Refereed]

  Introduction scientific journal


• パターン化脂質膜を基盤とした人工生体膜の創出

  森垣 憲一

  May 2016, 表面科学, 37(5) (5), 230 - 234, Japanese

  [Refereed][Invited]

  Introduction scientific journal


• 光重合性脂質を用いたパターン化モデル生体膜の創製

  森垣 憲一

  Oct. 2015, 高分子, 64(10) (10), 653 - 654, Japanese

  [Invited]

  Introduction scientific journal


• Functional analysis of the biological membrane by using a substrate-supported model membrane

  Morigaki K

  Substrate supported phospholipid bilayers are model systems of the biological membrane that offer possibilities for integration (micro-patterning) and sensitive analyses. We developed hybrid membranes composed of polymerized and fluid bilayers. Since the polymeric bilayer provides a robust framework for incorporating various types of lipid membranes, it can be applied to study the functions of membrane-bound proteins and peptides. Amyloid fibril formation has been studied using micro-patterned supported membrane. Since the hybrid membranes are both stable and functional, they should provide a new avenue for generating robust model systems for the basic study of membrane functions as well as biomedical applications.

  THE MEMBRANE SOCIETY OF JAPAN, Nov. 2012, Membrane, 37(6) (6), 276 - 281, Japanese

  [Refereed]

  Introduction scientific journal


• Supported Lipid Bilayer

  FURUKAWA Kazuaki, NABIKA Hideki, MURAKOSHI Kei, MORIGAKI Kenichi, TERO Ryugo

  日本表面科学会, 10 Apr. 2009, Journal of the Surface Science Society of Japan, 30(4) (4), 207 - 218, Japanese


• Micropatterned model biological membranes composed of polymerized and fluid lipid bilayers

  Kenichi Morigaki

  Jun. 2008, BIOINTERPHASES, 3(2) (2), FA85 - FA89, English

  [Refereed]

  Introduction scientific journal


• 膜融合ペプチドにより引き起こされるリポソームと脂質平面膜の相互作用の全反射蛍光顕微鏡観察(The effect of membrane fusion peptides on interactions between liposomes and supported planar lipid bilayers: TIRF observations)

  稲葉 岳彦, 達 吉郎, 森垣 憲一

  (一社)日本生物物理学会, Nov. 2007, 生物物理, 47(Suppl.1) (Suppl.1), S178 - S178, English


• 流動性脂質およびポリマー脂質からなる基板支持型コンポジット二分子膜(Composite membranes of fluid and polymerized phospholipid bilayers on solid substrate)

  岡崎 敬, 稲葉 岳彦, 達 吉郎, 森垣 憲一

  (一社)日本生物物理学会, Nov. 2007, 生物物理, 47(Suppl.1) (Suppl.1), S178 - S178, English


• Fatty acid vesicles

  Kenichi Morigaki, Peter Walde

  Apr. 2007, CURRENT OPINION IN COLLOID & INTERFACE SCIENCE, 12(2) (2), 75 - 80, English

  [Refereed]

  Book review


• Surfactant assisted formation of substrate supported planar lipid bilayers

  Shigeki Kimura, Takehiko Inaba, Takashi Okazaki, Kenichi Morigaki

  Jan. 2007, BIOPHYSICAL JOURNAL, 422A - 422A, English

  Summary international conference

• 生物模倣技術と新材料･新製品開発への応用

  Morigaki K

  Others, 技術情報協会, Jul. 2014, Japanese, 固体基板表面に形成された人工生体膜の特性と応用展開

  Scholarly book


• Handbook of Biofunctional Surfaces

  Morigaki K

  Others, Pan Stanford Publishing, May 2012, English, Micropatterned model biological membranes on a solid surface

  Scholarly book


• BRAIN TECHNO NEWS 142

  今石 浩正, 後藤 達志, UNO TOMOHIDE, MORIGAKI KENICHI

  Others, 農研機構 生研センター, 2010, Japanese, 食品の安全性評価へのバイオセンサーの利用

  Scholarly book


• Advances in Planar Lipid Bilayers andLiposomes

  Morigaki K

  Others, Elsevier 9, 2009, English, Micropatterned lipid bilayer membranes on solid substrates

  Scholarly book

• 基板表⾯におけるチラコイド膜のパターン化再構成

  髙木洸希, 森垣憲一

  第３２回光合成セミナー2025：反応中心と色素系の多様性, Jun. 2025, Japanese

  Oral presentation


• パターン化脂質膜とナノ空間を融合した人工生体膜の創成

  森垣憲一

  日本膜学会第４７年会, Jun. 2025, Japanese

  Public discourse


• 人工膜とナノ空間を用いた生体分子・膜小胞解析技術の開発

  吉村優, 長塚ななみ, 森垣憲一

  化学とマイクロ・ナノシステム学会 第51回研究会（CHEMINAS 51), May 2025, Japanese

  Poster presentation


• Model membrane arrays generated by self-spreading of lipid bilayers in a polymerized lipid bilayer framework

  Masako Fujii, Kenichi Morigaki

  American chemical society spring 2025（ACS Spring 2025）, Mar. 2025, English, International conference

  Oral presentation


• Reconstitution of rhodopsin photoreceptor into a supported lipid bilayer using peptide nanodisc

  Masato Koezuka, Kenichi Morigaki, Fumio Hayashi

  American chemical society spring 2025（ACS Spring 2025）, Mar. 2025, English, International conference

  Oral presentation


• Biomimetic membrane system composed of patterned lipid bilayers and nanometer-sized fluidic channels

  Kenichi Morigaki

  American chemical society spring 2025（ACS Spring 2025）, Mar. 2025, English, International conference

  [Invited]

  Invited oral presentation


• RECONSTITUTION AND ANALYSIS OF MEMBRANE PROTEINS IN A MICRO-PATTERNED MODEL MEMBRANE

  Kenichi Morigaki, Masato Koezuka, Fuko Kueda, Yasushi Tanimoto, Fumio Hayashi

  Biophysical Society2025(BPS2025), Feb. 2025, English, International conference

  [Invited]

  Invited oral presentation


• New studies on biomembranemolecular machinery

  Kenichi Morigaki

  First International Core-to-Core Symposium on BiomembraneMolecular Machinery, Dec. 2024, English, International conference

  [Invited]

  Invited oral presentation


• 生体膜の2次元液体と微小空間を再現する集積化人工膜の開発

  森垣 憲一

  第59回分子ロボティクス研究会, Dec. 2024, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• Spontaneous formation of supported lipid membranes for generating a model biological membrane array

  Kenichi Morigaki, Masako Fujii, Fuyuko Tamura, Yasushi Tanimoto, Misato Yamada, Fumio, Hayashi

  Symposium on “Engineering Science of Membranes and Particles, Oct. 2024, English, International conference

  [Invited]

  Invited oral presentation


• Integrated Model System of the Biological Membrane on Solid Surface

  Kenichi Morigaki

  PRiME 2024, Oct. 2024, English, International conference

  [Invited]

  Invited oral presentation


• 拠点形成事業「生体膜の分子機構を理解し活用するための国際研究拠点形成」の紹介

  森垣 憲一

  岐阜大学・神戸大学・国立がん研究センター 生体膜研究交流会, Sep. 2024, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• Reconstitution of thylakoid membrane in a patterned polymeric lipid bilayer scaffold

  Koki Takagi, Yuka Kusunoki, Daisuke Takagi, Sophie A. Meredith, Ashley M. Hancock, Stephen D. Evans, Peter G. Adams, Kenichi Morigaki

  2nd Asia-Oceania International Congress on Photosynthesis (2nd AOICP), Sep. 2024, English, International conference

  Oral presentation


• Membrane binding and regulation of a secreted glycoprotein Wnt studied using a micro-patterned model membrane

  Yu Yoshimura, Masato Koezuka, Koichiro M. Hirosawa, Hiromune Ando, Kenichi G.N. Suzuki, Rinshi S. Kasai, Kenichi Morigaki

  Glyco-core Symposium 2024, Jul. 2024, English, International conference

  Poster presentation


• 基板表⾯におけるチラコイド膜の再構成

  髙木 洸希, 楠 祐佳, 高木 大輔, 森垣 憲一

  日本光合成学会, Jun. 2024, Japanese, Domestic conference

  Poster presentation


• 光重合性脂質(Diyne-PC)を光収穫層とするエネルギー移動系の構築

  ⻑⾕川 馨、近藤 政晴、森垣 憲⼀、出⽻ 毅久

  日本光合成学会, Jun. 2024, Japanese, Domestic conference

  Poster presentation


• Reconstituting G protein-coupled receptors into a supported lipid bilayer using meta-stable peptide nanodiscs

  Fumio Hayashi, Masato Koezuka, Kenich Morigaki

  IUPAB2024, Jun. 2024, English, International conference

  Poster presentation


• Integrated model membrane arrays generated by self-spreading of lipid bilayers

  Masako Fujii, Kenichi Morigaki

  IUPAB2024, Jun. 2024, English, International conference

  Poster presentation


• Nanofluidic model cell membrane platform for molecular analysis of membrane-bound proteins

  Yu Yoshimura, Nanami Nagatsuka, Ryota Komatsu, Shin-ichi Yusa, Kenichi Morigaki

  IUPAB2024, Jun. 2024, English, International conference

  Poster presentation


• 人工生体膜とナノ空間を利用した一分子計測技術の開発

  ⻑塚 ななみ, 宮田 優里, 東原 太成, 肥塚 雅人, 林 文夫, 森垣 憲一

  化学とマイクロ・ナノシステム学会 第49回研究会（CHEMINAS 49）, Jun. 2024, Japanese, Domestic conference

  Poster presentation


• Model biological membrane composed of patterned lipid bilayer and nanometer-sized confinement

  Kenichi Morigaki

  ACS Spring 2024, Mar. 2024, English, International conference

  [Invited]

  Invited oral presentation


• Nanofluidic supported model cell membrane for single molecular analysis of membrane-bound proteins

  Yu Yoshimura, Ryota Komatsu, Shin-ichi Yusa, Kenichi Morigaki

  若手フロンティア研究会2023, Dec. 2023, Japanese, Domestic conference

  Poster presentation


• 支持脂質二分子膜にGPCR を配向性を制御して組み込む

  肥塚雅人, 森垣憲一, 林文夫

  若手フロンティア研究会2023, Dec. 2023, Japanese, Domestic conference

  Poster presentation


• Model Membrane Arrays Prepared by Self-Spreading of Lipid Bilayers in Preformed Polymeric Lipid Bilayer Channels

  Masako Fujii, Kenichi Morigaki

  若手フロンティア研究会2023, Dec. 2023, Japanese, Domestic conference

  Poster presentation


• Model Membrane Arrays Prepared by Self-Spreading of Lipid Bilayers in Preformed Polymeric Lipid Bilayer Channels

  Masako Fujii, Kenichi Morigaki

  16th International Symposium on Nanomedicine (ISNM2023), Nov. 2023, English, International conference

  Poster presentation


• Surface Modification of PDMS with Amphiphilic Random Copolymer for Nanofluidic Devices

  Yu Yoshimura, Ryota Komatsu, Shin-ichi Yusa, Kenichi Morigaki

  16th International Symposium on Nanomedicine (ISNM2023), Nov. 2023, English, International conference

  Poster presentation


• Lipid Membrane Micro and Nanotechnology

  Kenichi Morigaki

  36th International Microprocesses and Nanotechnology Conference (MNC 2023), Nov. 2023, English, International conference

  [Invited]

  Invited oral presentation


• Self-spreading lipid bilayers in preformed polymeric lipid bilayer channels

  Masako Fujii, Kenichi Morigaki

  第61回日本生物物理学会年会, Nov. 2023, English, Domestic conference

  Poster presentation


• A biomimetic molecular recognition platform based on functionalized lipid bilayer membrane

  Yuuri Miyata, Nanami Nagatsuka, Masato Koezuka, Fumio Hayashi, Kenichi Morigaki

  第61回日本生物物理学会年会, Nov. 2023, English, Domestic conference

  Poster presentation


• Characterization of artificial thylakoid membrane supported by 2-D lattice of polymerized lipid bilayer by Cryogenic spectral microscopy

  Hayata Sakai, Koki Takagi, Shen Ye, Kenichi Morigaki, Yutaka Shibata

  第61回日本生物物理学会年会, Nov. 2023, English, Domestic conference

  Poster presentation


• Single molecular diffusion process of G protein transducin on rhodopsin dimer rows

  Hayato Yamashita, Akihiro Tsuji, Fumio Hayashi, Kenichi Morigaki, Masashi Fujii, Akinori Awazu, Masayuki Abe

  第61回日本生物物理学会年会, Nov. 2023, English, Domestic conference

  Poster presentation


• Reconstituting GPCR into supported lipid bilayer with controlled orientation. [I] Prebinding of the G protein transducin

  Fumio Hayashi, Masato Koezuka, Kenichi Morigaki

  第61回日本生物物理学会年会, Nov. 2023, English, Domestic conference

  Poster presentation


• 支持脂質二層膜にGPCR を方向性を制御して組込む [II] GPCR のC 末特異性Fab´を使って

  肥塚 雅人, 森垣 憲一, 林 文夫

  第61回日本生物物理学会年会, Nov. 2023, English, Domestic conference

  Oral presentation


• 人工膜とナノ空間を用いた膜結合分子の動的挙動の計測

  長塚 ななみ, 宮田 優里, 吉村 優, 肥塚 雅人, 森垣 憲一

  第61回日本生物物理学会年会, Nov. 2023, English, Domestic conference

  Oral presentation


• 膜タンパク質の一分子解析のためのナノフルイディクス基板支持モデル細胞膜

  吉村 優, 小松 亮太, 遊佐 真一, 森垣 憲一

  第61回日本生物物理学会年会, Nov. 2023, English, Domestic conference

  Oral presentation


• SELF-SPREADING OF LIPID BILAYERS IN PREFORMED POLYMERIC LIPID BILAYER CHANNELS

  Masako Fujii, Kenichi Morigaki

  First International STIMULUS Conference(9/28.29), Sep. 2023, English, International conference

  Poster presentation


• Surface Modification of PDMS with Amphiphilic Random Copolymer for Nanofluidic Devices

  Yu Yoshimura, Ryota Komatsu, Shin-ichi Yusa, Kenichi Morigaki

  First International STIMULUS Conference, Sep. 2023, English, International conference

  Poster presentation


• Self-spreading lipid bilayers in preformed polymeric lipid bilayer channels

  Masako Fujii, Kenichi Morigaki

  13th International Congress on Membranes and Membrane Processes (ICOM2023), Jul. 2023, English, International conference

  Poster presentation


• Model biological membrane combining patterned lipid bilayer and nanometer-sized confinement

  Kenichi Morigaki

  13th International Congress on Membranes and Membrane Processes (ICOM2023), Jul. 2023, English, International conference

  Oral presentation


• パターン化脂質膜とナノ空間を融合したモデル生体膜

  森垣憲一

  生体膜デザインコンファレンス, Dec. 2022, Japanese, Domestic conference

  [Invited]

  Keynote oral presentation


• パターン化モデル生体膜を用いた膜タンパク質

  森垣 憲一

  第95回日本生化学会大会, Nov. 2022, English, Domestic conference

  [Invited]

  Invited oral presentation


• Micropatterned model membrane composed of polymerized and natural lipid bilayers

  Kenichi Morigaki

  第60回日本生物物理学会年会, Sep. 2022, English, Domestic conference

  [Invited]

  Invited oral presentation


• Patterned monolayer/bilayer hybrid membrane composed of polymerized and natural lipids

  Yasushi Tanimoto, Fumio Hayashi, Kenichi Morigaki

  第60回日本生物物理学会年会, Sep. 2022, English, Domestic conference

  Poster presentation


• Single molecule observation of G protein transducin on rhodopsin cluster by high-speed AFM

  Hayato Yamashita, Akihiro Tsuji, Fumio Hayashi, Kenichi Morigaki, Masashi Fujii, Akinori Awazu, Kazuhiko Hoshikaya, Masayuki Abe

  第60回日本生物物理学会年会, Sep. 2022, English, Domestic conference

  Poster presentation


• Reconstitution of photoreceptor rhodopsin into a patterned model membrane using peptide nanodisc

  Masato Koezuka, Fuko Kueda, Fumio Hayashi, Kenichi Morigaki

  第60回日本生物物理学会年会, Sep. 2022, English, Domestic conference

  Poster presentation


• Reconstitution of exosomes into a patterned model membrane

  Yu Yoshimura, Ayane Sugimachi, Fumio Hayashi, Koichiro M. Hirosawa, Rinshi S. Kasai, Kenichi G. N. Suzuki, Kenichi Morigaki

  第60回日本生物物理学会年会, Sep. 2022, English, Domestic conference

  Poster presentation


• Actin network assembly on a patterned model membrane

  Yosuke Yamazaki, Yuri Miyata, Kenichi Morigaki, Makito Miyazaki

  第60回日本生物物理学会年会, Sep. 2022, English, Domestic conference

  Poster presentation


• Hybrid membrane of reconstituted thylakoid membrane and patterned polymeric lipid bilayer scaffold

  Yuka Kusunoki, Takuro Yoneda, Sophie A. Meredith, Ashley M. Hancock, Daisuke Takagi, Seiji Akimoto, Stephen D. Evans, Peter G. Adams, Kenichi Morigaki

  ACS spring 2022, Mar. 2022, English

  [Invited]

  Invited oral presentation


• Reconstitution of thylakoid membrane in a patterned model membrane

  Yuka Kusunoki, Daisuke Takagi, Seiji Akimoto, Syouhei Maekawa, Kenichi Morigaki

  第59回日本生物物理学会年会, Nov. 2021, English, Domestic conference

  Oral presentation


• Functional reconstitution of dopamine D2 receptor into a supported model membrane in a

  Ayane Sugimachi, Rurika Nagai, Kenichi Suzuki, Fumio Hayashi, Rinshi Kasai, Kenichi Morigaki

  第59回日本生物物理学会年会, Nov. 2021, English, Domestic conference

  Oral presentation


• Reconstitution of rhodopsin into a patterned model membrane using nanodisc formed from

  Fuko Kueda, Fumio Hayashi, Kenichi Morigaki

  第59回日本生物物理学会年会, Nov. 2021, English, Domestic conference

  Oral presentation


• Functional reconstitution of dopamine D2 receptor into a supported model membrane in a nanometric confinement

  Ayane Sugimachi

  ACS spring 2021 Web開催, Apr. 2021, English, International conference

  Poster presentation


• Model biological membrane reconstituted in a nanometric space

  Kenichi Morigaki

  ACS spring 2021 Web開催, Apr. 2021, English, International conference

  [Invited]

  Invited oral presentation


• 人工膜とナノ空間を用いた細胞膜構造の再構成と機能解析

  森垣 憲一

  日本化学会 第101春季年会 Web開催, Mar. 2021, Japanese

  [Invited]

  Invited oral presentation


• Lateral diffusion in lipid bilayers biased by optical forces

  Yuto Ishihara, Tutsunori Kishimoto, Fuko Kueda, Suguru N. Kudoh, Kenichi Morigaki, Chie Hosokawa

  The 58th Annual Meeting of the Biophysical Society of Japan, Sep. 2020, English, Japan, Domestic conference

  Poster presentation


• Relationship between function and dynamics of rhodopsin using normal mode analysis

  Yukito Kaneshige, Masashi Fujii, Fumio Hayashi, Kenichi Morigaki, Hayato Yamashita, Akinori Awazu

  The 58th Annual Meeting of the Biophysical Society of Japan, Sep. 2020, English, Japan, Domestic conference

  Poster presentation


• Dynamic process of G protein transducin on rhodopsin cluster observed by high-speed AFM

  Kazuhiko Hoshikaya, Hayato Yamashita, Fumio Hayashi, Kenichi Morigaki, Masashi Fujii, Akinori Awazu, Masayuki Abe

  The 58th Annual Meeting of the Biophysical Society of Japan, Sep. 2020, English

  Poster presentation


• Investigation of relationships between amyloid fibril formation and phospholipid bilayer destruction

  Hiroki Takayama, Kaori Mageshi, Kenichi Morigaki, Eri Chatani

  The 58th Annual Meeting of the Biophysical Society of Japan, Sep. 2020, English, Japan, Domestic conference

  Poster presentation


• Hybrid photosynthetic system of natural plant thylakoids and synthetic lipids reconstituted into a supported microscale membrane array

  Kenichi Morigaki

  ACS National meeting ＆ Expo WEB開催, Mar. 2020, English, Pennsylvania Convention Center, International conference

  Invited oral presentation


• パターン化人工膜への膜タンパク質の再構成

  永井るりか, 谷本泰士, 笠井倫志, 鈴木健一, 林 文夫, 森垣憲一

  光・量子デバイス研究会, Jan. 2020, Japanese, 姫路・西はりま地場産業センター, Domestic conference

  Oral presentation


• Patterned model biological membrane on the solid substrate:Potenti als foｒ biophysical studies and biomedical applications

  Kenichi Morigaki

  Okinawa Colloids 2019, Nov. 2019, English, 沖縄 万国津梁館, International conference

  [Invited]

  Invited oral presentation


• Phosphorylatedion of rhodopsin photoreceptor and arrestin binding studied in a patterned model membrane.

  杭田 芙子

  シンポジウム「理論と実験」2019, Oct. 2019, Japanese, 広島大学, Domestic conference

  Poster presentation


• Reconstitution of a photosynthetic system into a two-dimensional patterned model membrane

  TakuroYoneda, Yasushi Tanimoto, Daisuke Takagi, Kenichi Morigaki

  日米二国間セミナー, Oct. 2019, English, 京都国際交流会館, International conference

  Poster presentation


• パターン化モデル膜を用いたロドプシンリン酸化とアレスチン結合の解析

  杭田 芙子, 谷本 泰士, 林 文夫, 森垣 憲一

  第57回日本生物物理学会年会, Sep. 2019, English, 宮崎県・シーガイヤコンベンションセンター, Domestic conference

  Poster presentation


• 細胞膜断片ブレブを用いたモデル生体膜への膜タンパク質再構成

  永井 るりか, 谷本 泰士, 笠井 倫志, 鈴木 健一, 林 文夫, 森垣 憲一

  第57回日本生物物理学会年会, Sep. 2019, English, 宮崎県・シーガイヤコンベンションセンター, Domestic conference

  Poster presentation


• リン酸化ロドプシン･アレスチン複合体は視細胞円板膜切れ込み部に集まる

  林 文夫, 杭田 扶子, 森垣 憲一, 妹尾 圭司

  第57回日本生物物理学会年会, Sep. 2019, English, 宮崎県・シーガイヤコンベンションセンター, Domestic conference

  Poster presentation


• ロドプシンクラスター上におけるトランスデューシンの動的過程の高速AFM観察

  干鰯谷 和彦, 谷本 泰士, 山下 隼人, 森垣 憲一, 林 文夫, 阿部 真之

  第57回日本生物物理学会年会, Sep. 2019, English, 宮崎県・シーガイヤコンベンションセンター, Domestic conference

  Poster presentation


• インクジェット塗布を用いたパターン化人工生体膜の開発

  谷本 泰士, 山田 美紗登, 林 文夫, 森垣 憲一

  第57回日本生物物理学会年会, Sep. 2019, English, 宮崎県・シーガイヤコンベンションセンター, Domestic conference

  Poster presentation


• 網膜桿体細胞内円盤膜上での脂質-光受容タンパク質秩序形成の数理モデル

  金重 先人, 谷本 泰士, 西森 拓, 森垣 憲一, 林 文夫, 粟津 暁紀

  第57回日本生物物理学会年会, Sep. 2019, English, 宮崎県・シーガイヤコンベンションセンター, Domestic conference

  Poster presentation


• ガラス基板表面におけるチラコイド膜の再構成と光合成機能解析

  米田 卓郎, 谷本 泰士, 高木 大輔, 森垣 憲一

  第57回日本生物物理学会年会, Sep. 2019, English, 宮崎県・シーガイヤコンベンションセンター, Domestic conference

  Poster presentation


• Micropatterned model biological membrane: Potentials for biophysicalstudies and biomedical applications

  Kenichi Morigaki

  Bio-Nano-Summer school 2019, Aug. 2019, English, Kleinwalsertal, Austria, International conference

  Keynote oral presentation


• Micropatterned model biological membrane: Potentials for biophysicalstudies and biomedical applications

  Kenichi Morigaki

  MNP Reserch Seminar, Jul. 2019, English, リーズ大学(イギリス), International conference

  Public discourse


• パターン化人工生体膜を用いた光合成機能評価技術の開発

  米田 卓郎, 谷本 泰士, 高木 大輔, 森垣 憲一

  第10回日本光合成学会年会およびシンポジウム, May 2019, Japanese, 京都産業大学 むすびわざ館, Domestic conference

  Poster presentation


• Self-spreading of a phospholipid bilayer in the scaffold of polymerized lipid bilayer

  Kenichi Morigaki, Fuyuko Tamura, Yasushi Tanimoto, Rurika Nagai, Misato Yamada, Fumio Hayashi

  ACS Spring 2019 National Meeting & Exposition, Apr. 2019, English, Orange Convention Cente, International conference

  [Invited]

  Invited oral presentation


• Patterned lipid bilayer combined with a nanometric gap structure as a versatilemodel of the biological membrane

  Kenichi Morigaki

  10th International Workshop on Nanostructures and Nanoelectronics, Mar. 2019, English, 東北大学 電気通信研究所, International conference

  [Invited]

  Invited oral presentation


• Model Biological Membranes on a Solid Substrate:Potential for Biophysical Studies and Biomedical Applications

  Kenichi Morigaki

  Seminar, Mar. 2019, English, コーネル大学化学生物工学科, International conference

  [Invited]

  Invited oral presentation


• Model Biological Membranes on a Solid Substrate:Potential for Biophysical Studies and Biomedical Applications

  Kenichi Morigaki

  the department of Biochemistry and Cell & Developmental Biology Seminar Series, Mar. 2019, English, コーネル大学医学部, International conference

  [Invited]

  Invited oral presentation


• Enhanced membrane disruption by Aβ1-40 under weak acidic conditions

  ○Kaori Mageshi, Naoki Yamamoto, Ryota Komatsu, Kenichi Morigaki, Masatomo So, Yuji Goto, Eri Chatani

  Ulm Meeting on “Biophysics of Amyloid Formation”, Feb. 2019, English, Ulm University, Germany, International conference

  Poster presentation


• Substrate-supported model biological membrane with controlled two-dimensional and three-dimensional structures

  小林 佐和子, 小松 亮太, 森垣 憲一

  日本生物物理学会第56回年会, Sep. 2018, English, 岡山大学 津島キャンパス, Domestic conference

  Poster presentation


• Single molecule observation of membrane proteins in a model biological membrane integrated with a nanometric gap structure

  小松 亮太, 谷本 泰士, HAYASHI FUMIO, 森垣 憲一

  日本生物物理学会第56回年会, Sep. 2018, English, 岡山大学 津島キャンパス, Domestic conference

  Oral presentation


• Reconstitution and functional analysis of thylakoid membrane on a glass substrate

  米田 卓郎, 谷本 泰士, 高木 大輔, 森垣 憲一

  日本生物物理学会第56回年会, Sep. 2018, English, 岡山大学 津島キャンパス, Domestic conference

  Oral presentation


• Raftophilicity and aggregation of membrane proteins in the photo-transduction

  森垣 憲一, 谷本 泰士, 山下 隼人, 粟津 暁紀, HAYASHI FUMIO

  日本生物物理学会第56回年会, Sep. 2018, English, 岡山大学 津島キャンパス, Domestic conference

  [Invited]

  Invited oral presentation


• Observing the interaction between rhodopsin cluster and transducin by high-speed AFM

  谷本 泰士, 山下 隼人, 野村 健人, 阿部 真之, HAYASHI FUMIO, 森垣 憲一

  日本生物物理学会第56回年会, Sep. 2018, English, 岡山大学 津島キャンパス, Domestic conference

  Poster presentation


• Direct reconstitution of membrane proteins from cell membrane blebs into a model biological membrane

  永井 るりか, 谷本 泰士, 笠井 倫志, 鈴木 健一, 林 文夫, 森垣 憲一

  日本生物物理学会第56回年会, Sep. 2018, English, 岡山大学 津島キャンパス, Domestic conference

  Poster presentation


• Development of complex and functional model membranes on solid substrate

  Kenichi Morigaki

  International Symposium on Science and Technology of MolecularAssemblies, Sep. 2018, English, 愛媛大学, International conference

  [Invited]

  Invited oral presentation


• A mathematical model of pattern formation of lipid-photoreceptor proteins on disk membranes of retinal cells.

  金重 先人, 粟津 暁紀, 西森 拓, HAYASHI FUMIO, 森垣 憲一, 谷本 泰士

  日本生物物理学会第56回年会, Sep. 2018, English, 岡山大学 津島キャンパス, Domestic conference

  Poster presentation


• 弱酸性条件におけるAβ1-40のアミロイド線維形成と脂質膜破壊

  曲師 香緒里, 山本 直樹, MORIGAKI KENICHI, CHATANI ERI

  第18回日本蛋白質科学会, Jun. 2018, Japanese, 朱鷺メッセ, Domestic conference

  Poster presentation


• Reconstituting Membrane Proteins in a Patterned Model Membrane

  Kenichi Morigaki

  Proteins in Artificial Membranes, May 2018, English, Austrian Institute of Technology, International conference

  [Invited]

  Invited oral presentation


• Patterned Model Biological Membrane on the Solid Substrate

  Kenichi Morigaki

  BIOELECTROCHEMISTRY AND MORE………2018, May 2018, English, CEST, International conference

  [Invited]

  Invited oral presentation


• Single molecule detection of biomarker in the a nanometric gap- structure combined with fluid membranejunction

  Koji Ando, Fumio Hayashi, Kenichi Morigaki

  255th ACS National Meeting, Mar. 2018, English, Ernest N. Morial Convention Center、 New Orleans、 LA, International conference

  Oral presentation


• Raftophilicity of membrane proteins involved in phototransduction evaluated with by using a micropatterned model membrane

  Yasushi Tanimoto, Sakiko Kojima, Akinori Awazu, Fumio Hayashi, Kenichi Morigaki

  255th ACS National Meeting, Mar. 2018, English, Ernest N. Morial Convention Center、 New Orleans、 LA, International conference

  Oral presentation


• Patterned model biological membrane on the solid substrate

  Kenichi Morigaki

  9th International Workshop on Nanostructures andNanoelectronics, Mar. 2018, English, 東北大学 電気通信研究所, International conference

  [Invited]

  Invited oral presentation


• Nanometric gap structure for selective biosensing created with patterned lipid bilayer, silicone elastomer, and silica nanoparticles

  Kenichi Morigaki

  255th ACS National Meeting, Mar. 2018, English, Ernest N. Morial Convention Center、 New Orleans、 LA, International conference

  [Invited]

  Invited oral presentation


• パターン化人工膜を用いた膜シグナル伝達機構の再構成と定量的解析

  森垣 憲一, 谷本 泰士, 小嶋 佐妃子, 粟津 暁紀, HAYASHI FUMIO

  2017年度生命科学系学会合同年次大会(第40回日本分子生物学会), Dec. 2017, Japanese, 神戸ポートピアホテル, Domestic conference

  [Invited]

  Invited oral presentation


• 人工生体膜とポリマー材料を用いたナノ空間の創成

  小松 亮太, 小林 佐和子, 安藤 公二, 岩崎 泰彦, 遊佐 真一, 森垣 憲一

  化学とマイクロ･ナノシステム学会 第36回研究会, Oct. 2017, Japanese, 桐生市市民文化会館 スカイホール, Domestic conference

  Poster presentation


• ナノ空間と人工生体膜を組み合わせた1分子計測技術の開発

  安藤 公二, HAYASHI FUMIO, 森垣 憲一

  化学とマイクロ･ナノシステム学会 第36回研究会, Oct. 2017, Japanese, 桐生市市民文化会館 スカイホール, Domestic conference

  Poster presentation


• Patterned lipid bilayer on solid substrate as a versatile model system of the biiological membrane

  Kenichi Morigaki

  ICBZM2017, Oct. 2017, English, 東京大学本郷キャンパス, International conference

  [Invited]

  Invited oral presentation


• Controlling the distance between a patterned model membrane andthe substrate with polymer brushes

  Sawako Kobayashi, Fuyuko Tamura, Kazuhiko Iwasaki, KenichiMorigaki

  ICBZM2017, Oct. 2017, English, 東京大学本郷キャンパス, International conference

  Poster presentation


• パターン化人工膜を利用したＮＡＰ－２２の膜結合と凝集挙動解析

  小嶋 佐妃子, 谷本 泰士, HAYASHI FUMIO, 前川 昌平, 森垣 憲一

  日本生物物理学会第55回年会, Sep. 2017, English, 熊本大学, Domestic conference

  Poster presentation


• パターン化人工膜を用いた光シグナル伝達中における脂質ラフトの機能解析

  谷本 泰士, 小嶋 佐妃子, 粟津 暁紀, HAYASHI FUMIO, 森垣 憲一

  日本生物物理学会第55回年会, Sep. 2017, English, 熊本大学, Domestic conference

  Poster presentation


• Single-molecule immunoassay in the nanogap-junction with a fluid lipid bilayer

  Koji Ando, Kenichi Morigaki

  TETHMEM 2017, Aug. 2017, English, Schloß Schönbrunn Meetings & Events(オーストリア), International conference

  Poster presentation


• Roles of lipid rafts in phototransduction studied with a micropatterned model membrane

  Yasushi Tanimoto, Kenichi Morigaki

  TETHMEM 2017, Aug. 2017, English, Schloß Schönbrunn Meetings & Events(オーストリア), International conference

  Poster presentation


• Roles of lipid rafts in phototransduction studied with a micropatterned model membrane

  Yasushi Tanimoto, Kenichi Morigaki

  BioNano Summer-School, Aug. 2017, English, Darmstädter Haus(ドイツ), International conference

  Oral presentation


• Model membrane on a solid substrate:Crawling to grow from 2D into 3D structures

  Kenichi Morigaki

  BioNano Summer-School, Aug. 2017, English, Darmstädter Haus(ドイツ), International conference

  [Invited]

  Invited oral presentation


• Micropatterned model membrane to elucidate the funcitonal roles of lipid micro-domains in the signal transduction cascade

  Kenichi Morigaki

  TETHMEM 2017, Aug. 2017, English, Schloß Schönbrunn Meetings & Events(オーストリア), International conference

  [Invited]

  Invited oral presentation


• Membrane binding and aggregation of neuronal acidic protein of 22kDa (NAP-22) studied with a patterned model membrane

  Sakiko Kojima, Kenichi Morigaki

  TETHMEM 2017, Aug. 2017, English, Schloß Schönbrunn Meetings & Events(オーストリア), International conference

  Poster presentation


• Fabricating a nanometric gap junction by attaching a patterned lipid bilayer with PDMS via polymeric materials

  Ryota Komatsu, Kenichi Morigaki

  TETHMEM 2017, Aug. 2017, English, Schloß Schönbrunn Meetings & Events(オーストリア), International conference

  Poster presentation


• Fabricating a nanometric gap junction by attaching a patterned lipid bilayer with PDMS via polymeric materials

  Ryota Komatsu, Kenichi Morigaki

  BioNano Summer-School, Aug. 2017, English, Darmstädter Haus(ドイツ), International conference

  Oral presentation


• Development of model biological membranes for the functional reconstitution of membrane proteins

  Fuyuko Tamura, Kenichi Morigaki

  TETHMEM 2017, Aug. 2017, English, Schloß Schönbrunn Meetings & Events(オーストリア), International conference

  Poster presentation


• Development of model biological membranes for the functional reconstitution of membrane proteins

  Fuyuko Tamura, Kenichi Morigaki

  BioNano Summer-School, Aug. 2017, English, Darmstädter Haus(ドイツ), International conference

  Oral presentation


• Controlling the distance between a patterned model membrane and the substrate with polymer brushes

  Sawako Kobayashi, Kenichi Morigaki

  TETHMEM 2017, Aug. 2017, English, Schloß Schönbrunn Meetings & Events(オーストリア), International conference

  Poster presentation


• Controlling the distance between a patterned model membrane and the substrate with polymer brushes

  Sawako Kobayashi, Kenichi Morigaki

  BioNano Summer-School, Aug. 2017, English, Darmstädter Haus(ドイツ), International conference

  Oral presentation


• 人工膜を用いた膜シグナル伝達機構の再構成と定量的解析

  森垣 憲一

  「理研シンポジウム」細胞システムの動態と論理VIII, Apr. 2017, Japanese, 理化学研究所, Domestic conference

  [Invited]

  Invited oral presentation


• Single-molecule detection of biomarker molecules in a nanometric gapstructure with a fluid lipid membrane

  Koji Ando, Fumio Hayashi, Kenichi Morigaki

  253st ACS National Meeting and Exposition, Apr. 2017, English, San Francisco Convention Center, International conference

  Oral presentation


• Hybrid model membrane combining micropatterned lipid bilayer and hydrophilic polymer brush

  Kenichi Morigaki

  253st ACS National Meeting and Exposition, Apr. 2017, English, San Francisco Convention Center, International conference

  [Invited]

  Invited oral presentation


• Evaluating the interactions of lipid raft and proteins involved inphototransduction by using a micropatterned model membrane

  Yasushi Tanimoto, Sakiko Kojima, Fumio Hayashi, Kenichi Morigaki

  253st ACS National Meeting and Exposition, Apr. 2017, English, San Francisco Convention Center, International conference

  Oral presentation


• Patterned lipid bilayer on solid substrate as a versatile model systemof the biological membrane

  Kenichi Morigaki

  講演会, Mar. 2017, English, Sandia National Laboratories, International conference

  [Invited]

  Invited oral presentation


• Nanogap-junction with a fluid lipid bilayer for selective biosensing

  Kenichi Morigaki

  8th International Workshop on Nanostructures & Nanoelectronics, Mar. 2017, English, 東北大学, International conference

  [Invited]

  Invited oral presentation


• 人工生体膜とナノ空間を利用した1分子計測技術の開発

  Koji Ando, Fumio Hayashi, Kenichi Morigaki

  第54回日本生物物理学会, Nov. 2016, English, つくば国際会議場, Domestic conference

  Poster presentation


• 親水性高分子により基板との距離を制御した人工膜への膜タンパク質再構成

  Fuyuko Tamura, Yasushi Tanimoto, Yasuhiko Iwasaki, Fumio Hayashi, Yuki Sudo, Kenichi Morigaki

  第54回日本生物物理学会, Nov. 2016, English, つくば国際会議場, Domestic conference

  Poster presentation


• 視細胞円板膜上のロドプシン多量対クラスターが作る一過性メゾ領域

  HAYASHI FUMIO, 齋藤 夏美, 谷本 泰士, 森垣 憲一, 妹尾 圭司

  第54回日本生物物理学会, Nov. 2016, English, つくば国際会議場, Domestic conference

  [Invited]

  Invited oral presentation


• パターン化人工膜を利用したNAP-22 の膜結合と凝集挙動解析

  Sakiko Kojima, Yasushi Tanimoto, Fumio Hayashi, Shohei Maekawa, Kenichi Morigaki

  第54回日本生物物理学会, Nov. 2016, English, つくば国際会議場, Domestic conference

  Poster presentation


• パターン化人工膜を用いて脂質ラフトによる光シグナル伝達の制御機構を解明する

  Yasushi Tanimoto, Sakiko Kojima, Fumio Hayashi, Kenichi Morigaki

  第54回日本生物物理学会, Nov. 2016, English, つくば国際会議場, Domestic conference

  Poster presentation


• Evaluating the raftophilicity of rhodopsin in a patterned model membrane

  Kenichi Morigaki, Yasushi Tanimoto, Fumio Hayashi

  the 251st ACS National Meeting & Exposition, Mar. 2016, English, San Diego Convention Center, International conference

  [Invited]

  Invited oral presentation


• ﾊﾟﾀｰﾝ化脂質膜とナノ空間を融合した新規人口生体膜の創出

  森垣 憲一, 安藤 公二, 田邊 真志

  2015年真空・表面科学合同講演会, Dec. 2015, Japanese, つくば国際会議場, Domestic conference

  [Invited]

  Invited oral presentation


• Solid-state NMR and microscopic studies of synthetic mimic of GPI-anchored proteins

  Kaoru Nomura, Erisa Harada, Kenji Sugase, Keiko Shimamoto, Fumio Hayashi, Kenichi Morigaki

  Pacifichem 2015, Dec. 2015, English, ハワイコンベンションセンター, International conference

  Poster presentation


• Nanometic gap structure for the selective transport and detection of biological molecules

  Koji Ando, Masashi Tanabe, Kenichi Morigaki

  Pacifichem 2015, Dec. 2015, English, ハワイコンベンションセンター, International conference

  Poster presentation


• Evaluating the raftophilicity of rhodopsin in a patterned model membrane

  Yasushi Tanimoto, Fumio Hayashi, Kenichi Morigaki

  Pacifichem 2015, Dec. 2015, English, ハワイコンベンションセンター, International conference

  Poster presentation


• Mocro-/Nano-Compartments Between Substrate-Sopported Model Membrane and Silisone Elastomer

  Kenichi Morigaki

  国際シンポジウム「TethMem 2015」, Nov. 2015, English, Nanyang Technological University （ｼﾝｶﾞﾎﾟｰﾙ）, International conference

  [Invited]

  Invited oral presentation


• Hybrid model membrane combining micropatterned lipid bilayer andpolymer brushes

  Toshiki Nishimura, Kenichi Morigaki

  国際シンポジウム「TethMem 2015」, Nov. 2015, English, Nanyang Technological University （ｼﾝｶﾞﾎﾟｰﾙ）, International conference

  Poster presentation


• ﾊﾟﾀｰﾝ化モデル生体膜を用いた、光シグナル伝達に関わる膜タンパク質の脂質ラフト親和性解析

  谷本 泰士, 小嶋 佐妃子, 森垣 憲一, 林 文夫

  第53回日本生物物理学会年会, Sep. 2015, English, 金沢大学 角間キャンパス, Domestic conference

  Oral presentation


• ロドプシンはラフト親和性の短寿命ナノドメインを形成しながら拡散している

  HAYASHI FUMIO, 齋藤 夏美, 谷本 泰士, 森垣 憲一, 妹尾 圭司

  第53回日本生物物理学会年会, Sep. 2015, English, 金沢大学 角間キャンパス, Domestic conference

  Poster presentation


• 1分子観察によるガングリオシドのダイマー形成機構の解明

  鈴木 健一, 安藤 弘宗, 河村 奈緒子, 山崎 彩乃, 石田 秀治, 古川 鋼一, 森垣 憲一, 楠見 明弘, 木曽 真

  第53回日本生物物理学会年会, Sep. 2015, English, 金沢大学 角間キャンパス, Domestic conference

  Poster presentation


• 人工生体膜を利用した高感度生体分子計測技術

  森垣 憲一

  第14回ライフサイエンスワールド, May 2015, Japanese, 東京ビッグサイト, Domestic conference

  Public symposium


• Nanometric gap structure between substrate-supported model membrane and silicone elastomer

  森垣 憲一

  249th American Chemical Society National Exposition, Mar. 2015, English, Denver, CO, International conference

  [Invited]

  Invited oral presentation


• Micro-nano-compartments between substrate-supported model membrane andsilicone elastomer

  森垣 憲一

  The Joint Symposium of 9th International Symposium on Medical, Bio- and Nano-Electronics,and6th International Workshop on Nanostructures & Nanoelectronics, Mar. 2015, English, 東北大学電気通信研究所, International conference

  [Invited]

  Invited oral presentation


• 人工生体膜を利用した高感度生体分子計測技術

  森垣 憲一

  メディカルジャパン2015, Feb. 2015, Japanese, インテックス大阪, Domestic conference

  Public discourse


• 脂質二分子膜と高分子材料を融合したハイブリッド型人工膜の創製

  安藤 公二, 森垣 憲一

  日本生物物理学会 第52回年会, Sep. 2014, English, 札幌コンベンションセンター, Domestic conference

  Poster presentation


• モデル生体膜を用いたロドプシン光受容体の脂質ラフト親和性解析

  谷本 泰士, 森垣 憲一, 林 文夫

  日本生物物理学会 第52回年会, Sep. 2014, English, 札幌コンベンションセンター, Domestic conference

  Poster presentation


• パターン化人工膜を用いた膜タンパク質のラフト親和性解析

  森垣 憲一, 谷本 泰士, 岡田 文子, 林 文夫

  日本生物物理学会 第52回年会, Sep. 2014, English, 札幌コンベンションセンター, Domestic conference

  [Invited]

  Invited oral presentation


• Controlled distribution of lipids and proteins in a micropatterned model menbrane

  Morigaki K

  247th American Chemical Society National Exposition, Mar. 2014, English, Dallas Convention Center, International conference

  [Invited]

  Invited oral presentation


• マイクロパターン化モデル生体膜における脂質ドメインの空間的制御

  岡田 文子, 森垣 憲一

  第51回日本生物物理学会年会, Oct. 2013, Japanese, 国立京都国際会館, Domestic conference

  Poster presentation


• パターン化モデル生体膜へのロドプシンの再構成

  谷本 泰士, 森垣 憲一, 林 文夫

  第51回日本生物物理学会年会, Oct. 2013, Japanese, 国立京都国際会館, Domestic conference

  Poster presentation


• パターン化モデル生体膜と微小構造体を接合したナノ界面における分子認識と分子輸送

  森垣 憲一

  ソフトインターフェースの分子科学第10回公開ｼﾝﾎﾟｼﾞｳﾑ, Jul. 2013, Japanese, 東京大学大学院数理科学研究科, Domestic conference

  Poster presentation


• 固体基板表面にモデル生体膜を集積化する技術の開発

  森垣 憲一

  日本生物物理学会 第5回中国四国支部大会, May 2013, Japanese, ベネッセハウス・パークホール, Domestic conference

  Oral presentation


• インクジェット塗布技術を用いた人工生体膜の作製

  山田 美紗登, 森垣 憲一

  第60回応用物理学会学術講演会, Mar. 2013, Japanese, 神奈川工科大学, Domestic conference

  Oral presentation


• パターン化モデル生体膜と微小構造体を接合したナノ界面における分子認識と分子輸送

  森垣 憲一

  ソフトインターフェースの分子科学第9回公開ｼﾝﾎﾟｼﾞｳﾑ, Jan. 2013, Japanese, タワーホテル船堀, Domestic conference

  Oral presentation


• 微細パターン化モデル生体膜における膜結合タンパク質の濃縮の空間的制御

  Okada F, Morigaki K

  第50回日本生物物理学会年会, Sep. 2012, Japanese, 名古屋大学 東山キャンパス, Domestic conference

  Oral presentation


• パターン化モデル生体膜へのロドプシンの再構成

  Okada k, Morigaki K, F Hayashi

  第50回日本生物物理学会年会, Sep. 2012, Japanese, 名古屋大学 東山キャンパス, Domestic conference

  Poster presentation


• Stable and functional model biological membrane composed of polymericand fluid lipid bilayers

  Morigaki K

  Engineering Lipid Bilayers 2012, Sep. 2012, English, Weetwood Hall, International conference

  [Invited]

  Invited oral presentation


• ﾊﾟﾀｰﾝ化ﾓﾃﾞﾙ生体膜と微小構造体を接合したﾅﾉ界面における分子認識と分子輸送

  Morigaki K

  文部科学省科学研究費補助金・新学術領域研究「ソフトインターフェースの分子科学」（略称・ソフト界面）第８回領域会議, Jul. 2012, Japanese, 伝国の杜・置賜文化ホール, Domestic conference

  Oral presentation


• Coupling model cellular membranes with molecules, cells, and 3D objects

  Morigaki, K, Saito, M, Okazaki, T, Nakajima, Y, Tatsu, Y, Imaishi, H

  International Association of Colloid and Interface Scientists,Conference, May 2012, English, 仙台国際センター, International conference

  Oral presentation


• 食品の安全性評価用バイオセンシングシステムの開発

  今石 浩正, 森垣 憲一, 後藤 達志, 森 大気

  日本農芸学会2012年度大会, Mar. 2012, Japanese, 京都女子大学, Domestic conference

  [Invited]

  Invited oral presentation


• Surface functionalization of polymeric lipid bilayers for coupling with molecules, cells, and 3D objects

  Morigaki K, Saito M, Mizutani K, Okazaki T, Nakajima Y, Tatsu Y, Imaishi, H

  243 Meeting of the American Chemical Society, Mar. 2012, English, Sun diego convention center, International conference

  [Invited]

  Invited oral presentation


• P450 microarrays fabricated in microwells by the inkjet printing

  Kanemura E, Yamada M, Goto T, Tatsu Y, Imaishi H, Morigaki K

  243 Meeting of the American Chemical Society, Mar. 2012, English, Sun diego convention center, International conference

  Oral presentation


• ヒトP450発現大腸菌による食品化合物の網羅的な代謝試験

  清水 泰博, 松原 俊之, 後藤 達志, 森垣 憲一, 今石 浩正

  第34回日本分子生物学会, Dec. 2011, Japanese, パシフィコ横浜, Domestic conference

  Oral presentation


• ﾊﾟﾀｰﾝ化脂質二分子膜へのロドプシンの再構成

  Okada, K, Morigaki, K, Imaishi, H, Hyashi, H

  第49回日本生物物理学会年会, Sep. 2011, Japanese, 兵庫県立大学姫路書写キャンパス, Domestic conference

  Oral presentation


• ポリマー脂質二分子膜を基盤とした生体膜機能界面の創製

  森垣 憲一, 水谷 和幸, 齋藤 慎, 今石 浩正

  第60回高分子討論会, Sep. 2011, Japanese, 岡山大学津島キャンパス, Domestic conference

  [Invited]

  Invited oral presentation


• シトクロムＰ450と酵素センサーを積層化したＰ450マイクロアレイの開発

  森 芳直, 常 鋼, 後藤 達志, 今石 浩正, 森垣 憲一

  第84回日本生化学会大会, Sep. 2011, Japanese, 国立京都国際会館, Domestic conference

  Poster presentation


• インクジェット技術によるＰ４５０マイクロアレイ作製とケージド補酵素を用いた活性測定

  金村 絵美, 山田 美紗登, 後藤 達志, 今石 浩正, 森垣 憲一

  第84回日本生化学会大会, Sep. 2011, Japanese, 国立京都国際会館, Domestic conference

  Poster presentation


• クロルピリホス代謝に見られるヒトCYP2B6遺伝子多型の影響

  後藤 達志, 森 大気, 森垣 憲一, 宇野 知秀, 清水 泰博, 有澤 章, 今石 浩正

  日本農芸学会2011年度大会, Mar. 2011, Japanese, 日本農芸学会, 京都女子大, Domestic conference

  Poster presentation


• Model biological membranes composed of polymerized and fluid lipid bilayers

  Morigaki K

  241st Meeting of the American Chemical Society, Mar. 2011, English, Anaheim Convention Center, International conference

  [Invited]

  Invited oral presentation


• 微細加工チップを用いたシトクロムP450代謝活性測定技術

  金村 絵美, 水谷 和幸, 森垣 憲, 達 吉郎, 今石 浩正

  BMB2010（第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会）, Dec. 2010, Japanese, 神戸国際展示場, Domestic conference

  Poster presentation


• ヒトシトクロムP450 2A13 および2B6 によるフラノクマリン類の代謝

  後藤 達志, 森垣 憲一, 宇野 知秀, 清水 泰博, 有澤 章, 今石 浩正

  BMB2010（第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会）, Dec. 2010, Japanese, 神戸国際展示場, Domestic conference

  Poster presentation


• ヒトP450 を組込んだ新規薬物動態評価用バイオセンサーの開発

  後藤 達志, 森垣 憲一, 今石 浩正

  BMB2010（第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会）, Dec. 2010, Japanese, 神戸国際展示場, Domestic conference

  [Invited]

  Nominated symposium


• パターン化モデル生体膜を利用した膜タンパク質再構成技術の開発

  森垣 憲一, 岡田 佳祐, 水谷 和幸, 今石 浩正

  第36回 日本生体エネルギー研究会＆特定領域研究「革新的ナノバイオ」合同シンポジウム, Nov. 2010, Japanese, 大阪大学吹田キャンパス銀杏会館, Domestic conference

  Poster presentation


• Metabolic Activation of 5-Methoxypsoralen by Human CYP2A13 Variants Representing Single Nucleotied

  Goto T, Morigaki K, Uno T, Arisawa A, Imaishi H

  日本薬物動態学会第25回年会, Oct. 2010, English, 大宮ソニックシティー, Domestic conference

  Poster presentation


• Immobilization of cytochrome P450 on the solid surface

  水谷 和幸, 田和 圭子, 今石 浩正, 森垣 憲一

  日本生物物理学会第48回年会, Sep. 2010, English, 日本生物物理学会, 東北大学川内北キャンパス, Domestic conference

  Poster presentation


• Formation of substrate supported planar phospholipid bilayers in the presence of detergents

  岡田 佳祐, 今石 浩正, 森垣 憲一

  日本生物物理学会第48回年会, Sep. 2010, English, 日本生物物理学会, 東北大学川内北キャンパス, Domestic conference

  Poster presentation


• Composite membranes of polymeric and fluid lipid bilayers

  K Morigaki, T Okazaki, Y Tatsu, H Imaishi

  Soft Matter Physics 2010, Aug. 2010, English, 東京大学柏キャンパス, Domestic conference

  [Invited]

  Invited oral presentation


• パターン化モデル生体膜を利用した膜タンパク質再構成技術の開発

  森垣 憲一, 岡田 佳祐, 水谷 和幸, 今石 浩正

  本特定領域研究の第5回班会議「膜超分子モーターの革新的ナノサイエンス」, Jun. 2010, Japanese, 学習院大学創立百周年記念館, Domestic conference

  Oral presentation


• Micropatterned model membranes composed of polymerized and fluid lipid bilayers

  Morigaki K

  Biophysical Society 54th Annual Meeting, Feb. 2010, English, San FranciscoThe Moscone Center, International conference

  Poster presentation


• 固体基板表面におけるパターン化モデル生体膜の開発

  森垣 憲一

  第１４回情報バイオトロニクス研究会, Jan. 2010, Japanese, 東北大学, Domestic conference

  [Invited]

  Invited oral presentation


• 固体基板上でパターン化された脂質膜におけるリン脂質膜の収縮

  岡崎 敬, 森垣 憲一

  生物物理学会第４７回年会, Nov. 2009, English, 徳島文理大学, Domestic conference

  Poster presentation


• 界面活性剤を用いたリン脂質膜の固体基板への固定化手法

  森垣 憲一, 村 成, 稲葉 岳, 岡崎 敬, 川崎 隆史, 今石 浩正

  生物物理学会第４７回年会, Nov. 2009, English, 徳島文理大学, Domestic conference

  Oral presentation


• High-throughput Assay of Human P450 Activities by Using Immobilized Microsomes on Oxygen Sensor

  Gang Chang, 森垣 憲一, 達 吉郎, Uno Tomohide, 一色 邦夫, 今石 浩正

  薬物動態学会第２４回年会, Nov. 2009, English, 国立京都国際会議場, Domestic conference

  Oral presentation


• ヒトCYP2A13による食物由来成分の代謝における遺伝子多型の影響

  後藤 達志, 桧皮 貴史, 宇野 知秀, 一色 邦夫, 森垣 憲一, 今石 浩正

  第８２回日本生化学会大会, Oct. 2009, Japanese, 神戸ポートアイランド, Domestic conference

  Oral presentation


• シトクロムP450遺伝子多型CYP2C9*2とCYP2C9*3が食品酸化に与える影響

  平井 洋輔, 後藤 達志, 宇野 知秀, 一色 邦夫, 森垣 憲一, 今石 浩正

  第８２回日本生化学会大会, Oct. 2009, Japanese, 神戸ポートアイランド, Domestic conference

  Oral presentation


• ケージド化合物を用いたP450酵素活性の光制御

  水谷 和幸, 森垣 憲一, 達 吉郎, 一色 邦夫, 今石 浩正

  第８２回日本生化学会大会, Oct. 2009, Japanese, 神戸国際展示場, Domestic conference

  Poster presentation


• CYP1A1の遺伝子多型による食品成分の代謝活性変化

  桧皮 貴史, 後藤 達志, 宇野 知秀, 一色 邦夫, MORIGAKI KENICHI, 今石 浩正

  第８２回日本生化学会大会, Oct. 2009, Japanese, 神戸ポートアイランド, Domestic conference

  Oral presentation


• High-throughput Assay of Human P450 Activities by Using Immobilized Microsomes on Oxygen Sensor

  常 鋼, 森垣 憲一

  アドバンスセミナー 生物機能とナノテクノロジー メルシャン株式会社生物資源研究所, Aug. 2009, Japanese, メルシャン株式会社生物資源研究所, Domestic conference

  Poster presentation


• Induction of phase separation in micropatterned composite membranes of polymerized and fluid lipid bilayers

  Morigaki K

  American Chemical Society 237th National Meeting, Mar. 2009, English, (Salt Lake City), International conference

  [Invited]

  Invited oral presentation


• 集積型人工生体膜

  森垣 憲一

  ナノテク2009, Feb. 2009, Japanese, 東京ビッグサイト, Domestic conference

  Poster presentation


• ヒト薬物代謝酵素シトクロムP450を基板表面に固定化したバイオセンサーの開発

  水谷 和幸, 森垣 憲一, 田和 圭子, 今石 浩正

  日本農芸化学会関西支部例会（第４５８回講演会）, Feb. 2009, Japanese, 京大会館, Domestic conference

  Oral presentation


• Micropatterned phospholipid membranes on solid substrate as a platform for biotechnological and biomedical applications

  Morigaki K

  Asian Core Symposium-Nano and Biomedical Molecular Science, Feb. 2009, Japanese, (岡崎), Domestic conference

  [Invited]

  Invited oral presentation


• Model biological membranes on solid substrates

  Morigaki K

  The 3rd Workshop on Biological Applications of Plasma/Photon Processing, Jan. 2009, Japanese, Institute of Laser Engineering, Osaka University, Domestic conference

  [Invited]

  Invited oral presentation


• ヒトP450分子種、CYP2C18を発現した大腸菌による食品成分の代謝分析

  池川 朋代, 森内 寛, 付 学軍, 森垣 憲一, 一色 邦夫, 今石 浩正

  日本農芸化学会関西支部例会（第４５７回講演会）, Dec. 2008, Japanese, Domestic conference

  Oral presentation


• ヒトP450酵素発現大腸菌を用いた食品成分の代謝分析

  池川 朋代, 森内 寛, 付 学軍, 森垣 憲一, 一色 邦夫, 今石 浩正

  第３１回日本分子生物学会年会・第８１回日本生化学会大会合同大会 ＢＭＢ２００８ 2008.12.9-12, Dec. 2008, Japanese, 神戸国際展示場, Domestic conference

  Poster presentation


• ヒトCYP2C9 およびCYP2C19の遺伝子多型による薬物代謝活性変化の解析

  付 学軍, 平井 洋輔, 森内 寛, 池川 朋代, MORIGAKI KENICHI, 一色 邦夫, 今石 浩正

  日本農芸化学会関西支部例会（第４５７回講演会）, Dec. 2008, Japanese, Domestic conference

  Oral presentation


• ヒトCYP2C19およびCYP3A5の遺伝子多型による薬物代謝活性変化の解析

  付 学軍, 森内 寛, 池川 朋代, 宇野 知秀, MORIGAKI KENICHI, 一色 邦夫, 今石 浩正

  第３１回日本分子生物学会年会・第８１回日本生化学会大会合同大会 ＢＭＢ２００８ 2008.12.9-12, Dec. 2008, Japanese, 神戸国際展示場, Domestic conference

  Poster presentation


• ヒトCYP2C18酵素タンパク質の大腸菌内安定発現とその酵素化学的性質

  森内 寛, 付 学軍, 池川 朋代, 平井 洋輔, 森垣 憲一, 一色 邦夫, 今石 浩正

  第３１回日本分子生物学会年会・第８１回日本生化学会大会合同大会 ＢＭＢ２００８ 2008.12.9-12, Dec. 2008, Japanese, 神戸国際展示場, Domestic conference

  Poster presentation


• 固体基板上における人工生体膜の作製

  森垣 憲一, 達 吉郎

  第8回産学官連携フェア, Oct. 2008, Japanese, 北九州国際会議場, Domestic conference

  Poster presentation


• Integrated model biological membranes at the solid/ liquid interfaces

  Morigaki K

  JSPS-SNSF International Seminar on Membranomics, Sep. 2008, Japanese, 大阪大学 Σホール, Domestic conference

  [Invited]

  Invited oral presentation


• Micropatterned model biological membranes composed of polymerized and fluid lipid bilayers

  Morigaki K

  American Chemical Society 236th National Meeting, Aug. 2008, English, (Philadelphia), International conference

  [Invited]

  Invited oral presentation


• パターン化脂質膜に固定されたチトクロムＰ４５０を用いた酵素活性アッセイ法

  上田 佳弘, 森垣 憲一, 達 吉郎, 湯元 昇, 今石 浩正

  第３０回日本分子生物学会・第８０回日本生化学会大会 合同大会, Dec. 2007, Japanese, パシフィコ横浜, Domestic conference

  Poster presentation


• Immobilization of cytochrome P450 on micropatterned lipid bilayer substrates for the parallel assay of their activities

  上田 佳弘, 森垣 憲一, 達 吉郎, 湯元 昇, 今石 浩正

  日本生物物理学会第４５回年会, Dec. 2007, English, パシフィコ横浜, Domestic conference

  Poster presentation


• 人口膜とナノ空間を用いた生体分子・膜小胞解析技術の開発

  吉村優, 長塚ななみ, 森垣憲一

  化学とマイクロ・ナノシステム学会 第51回研究会（CHEMINAS 51）, Japanese

  Poster presentation

• パターン化人工膜とナノ空間を用いた細胞外小胞膜の再構成と膜タンパク質の解析

  森垣 憲一

  日本学術振興会, 科学研究費助成事業, 基盤研究(B), 神戸大学, 01 Apr. 2024 - 31 Mar. 2027


• 人工膜へのGタンパク質共役型受容体再構成法の確立

  林 文夫, 森垣 憲一

  日本学術振興会, 科学研究費助成事業, 基盤研究(B), 神戸大学, 01 Apr. 2022 - 31 Mar. 2025


• Investigation of photosynthetic machinery using patterned model membrane and nanoscopic analysis/ manipulation

  森垣 憲一, 出羽 毅久, 高木 大輔

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Kobe University, 07 Oct. 2021 - 31 Mar. 2025

  植物の光合成初期反応の場であるチラコイド膜は、多様な膜タンパク質群が2次元膜に配置された超分子系である。2次元膜における動的な分子分布や超分子形成は、光合成機能の調節に本質的に重要でありながら解析が困難であり充分には理解されていない。本研究は、世界的にも研究代表者のみが持つチラコイド膜のパターン化作製技術とリーズ大学の持つナノ計測・ナノ操作技術を組み合わせて、パターン化人工膜に再構成された光合成関連分子の膜内分布、エネルギー移動、電子伝達機能をナノメートルスケールで制御、定量評価する技術を開発することを目指している。初年度には、リーズ大学のDr. Peter G. Adams、Dr. Stephen D. Evansらと日本研究グループとの間でオンライン形式でのミニシンポジウムを開催し、お互いの技術を紹介するとともに今後の共同研究の進め方を詳細に議論した。また、神戸大学グループよりリーズ大学にパターン化ポリマー脂質膜およびチラコイド膜を送付して、チラコイド・脂質ハイブリッド膜の導入および電気泳動の予備実験を行った。日本国内においても、研究代表者と研究分担者（出羽、高木）の打合せおよび予備実験（導電性ガラス基板表面におけるパターン化ポリマー膜の作製）を行った。これらの検討により今後、リーズ大学と共同でチラコイド膜の動的な2次元分子分布による光合成機能調節を、人工膜を用いて再現しナノメートルスケールで詳細に解析する新規手法論を検討する準備が整った。


• Measuring molecular properties of membrane proteins using a synthetic cell micro-environment conposed of a model cell membrane and a nanometric space

  森垣 憲一, 林 文夫, 鈴木 健一, 笠井 倫志

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2020 - 31 Mar. 2023

  細胞膜の微小ドメイン構造（ラフト）は、膜タンパク質の機能調節に重要な役割を果たすと考えられている。研究代表者は、ラフト領域と非ラフト領域が明確に分かれているパターン化人工膜を用いて、膜タンパク質のラフト親和性を定量する手法を開発した。本研究は、人工膜と接する厚さ100nm以下のナノ空間に、細胞内のような高濃度のタンパク質やリガンドが存在する微小環境を再現し、それらと膜タンパク質との結合・解離を1分子蛍光観察で定量的に評価することを目的とする。2021年度には、①人工膜とナノ空間を一体化したバイオチップ作製、②パターン化人工膜への膜タンパク質の再構成、に関する技術開発を行った。 ①人工膜とナノ空間を一体化したバイオチップ作製：人工膜としては、光重合性リン脂質を光リソグラフィー技術でUV重合することでポリマー脂質膜を作製した。ナノ空間は、厚さの制御された接着層（高密度な親水性高分子鎖（高分子ブラシ）を被覆したシリカ微粒子）を用いて人工膜とPDMSを結合することで作製した。粒径100nmのナノ粒子を用いることで、流動性脂質膜部位の膜とPDMSの間に同程度の厚さを持つナノ空間が形成された。 ②パターン化人工膜への膜タンパク質の再構成：細胞由来の膜断片（blebs）を用いて、哺乳類細胞の膜タンパク質を人工膜に直接導入する技術を開発した。精神疾患に関わる重要なGPCRであるドーパミン受容体（D2R）をCHO細胞に発現し薬剤処理で膜断片を形成した。PDMSとパターン化膜との隙間（クレフト）において、区画内に高密度のD2Rが導入され、分子が2次元拡散していることを確認した。また、D2Rが生理的な活性を持つことを二量体形成寿命、アンタゴニストの結合などで示すことができた。今後、細胞小胞（エクソソーム）についてもクレフトにおける平面膜形成を検討して、膜タンパク質を1分子計測できる系を確立したい。


• 光受容体タンパク質が形成する超分子構造とシグナル伝達の分子動態機構の解明

  山下 隼人, 林 文夫, 森垣 憲一, 粟津 暁紀

  日本学術振興会, 科学研究費助成事業, 基盤研究(B), 大阪大学, 01 Apr. 2020 - 31 Mar. 2023

  本研究では、光受容体ロドプシン(Rh)の光照射に伴う1分子レベルでの構造変化やRhとシグナル伝達分子であるG蛋白質トランスデューシン(Gt)との相互作用動態を高速原子間力顕微鏡(高速AFM)で捉えることを目指しており、昨年度、そのためのAFM赤外光てこ光学系や暗室AFM装置環境の構築を行い、また比較的規模の大きな2量体列構造を持つRhの試料調製も行った。そこで本年度はこれらを用いて、まず光照射に伴うロドプシン(Rh)の構造変化の観察を試みた。暗所において2量体から成る列構造を形成するRhを高速AFMで観察すると2つの輝点が列状に並んでいる様子が観察されるが、その観察中に外部から緑色光を照射したところ、輝点の見え方に変化が生じた。Rhは暗状態、明状態(活性化状態)のそれぞれの結晶構造がすでに明らかにされており、それらによると暗状態から明状態への構造変化に伴ってRhの細胞質側(C末端側)表面のループ構造が2量体内で近づくことが分かっている。そこでこれらの結晶構造をもとに高速AFM像の再構成を行った結果、Rh2量体の２つの輝点が近づくことが示された。このことから、高速AFMで観察された輝点の変化は光照射に伴うRhの構造変化に由来するものであると考えられる。 次にRhとGtの相互作用の高速AFM観察を行うために、暗所でGtを機械的に投与するためのAFMシステムの構築を行った。Gtをマイクロ流路へ通して微量精密ポンプでAFM観察チャンバーへと転送する機構を作製し、完全暗所でGtを安定かつ迅速に投与できるようになった。また、Gtとの相互作用を観察するには、高活性な機能を保持したRh超分子クラスターの構築も必要になるため、パターン化膜技術のAFM計測への応用も試みた。専用の治具を作製することでAFM観察に用いる極微小ガラスステージの表面にポリマー膜からなるパターンを形成することに成功した。


• Reconstitution and functional analysis of photosynthetic machinery in a patterned model membrane

  森垣 憲一

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Kobe University, 01 Apr. 2019 - 31 Mar. 2021

  高等植物の光合成を行うチラコイド膜には、光合成電子伝達を通じて光エネルギーを化学エネルギーに変換する多様な分子群が2次元膜内に配置されている。本研究は、チラコイド膜における光合成関連タンパク質の動的な分子分布や超分子構造形成が光合成機能にどのように寄与するかを、分子レベルで定量的に解析できる革新的なin vitro評価技術として、固体基板表面に人工チラコイド膜を形成し高感度で機能解析する技術を開発した。そのため、光リソグラフィー技術によりパターン化形成されたポリマー脂質膜に、植物由来のチラコイド膜を導入した。チラコイド膜を高圧処理により断片化し、リン脂質ベシクルを融合促進剤として添加することでパターン化人工膜に導入できることを発見した。そして、チラコイド膜とリン脂質ベシクルの量比を調節することで、区画内に任意の密度でチラコイド膜を導入できることが分かった。電子受容体や阻害剤存在下でのクロロフィル蛍光計測から、光化学系II（PSII）の電子伝達活性の一部が保持されていることが示唆された。一方、低温蛍光観測の結果より、人工チラコイド膜において光化学系I（PSI）は複合体を維持しているものの、PSIIは反応中心と集光性クロロフィルタンパク質複合体（LHCII）が分離していることが示唆された。現在、低温での蛍光イメージング、高速AFMなどを用いて人工チラコイド膜における分子分布を検証しており、今後分子分布の精密計測・操作を通じて光合成機能における分子分布の役割を解明してゆきたい。


• 光受容体タンパク質が自発的に作る2次元秩序構造についての構成的研究

  森垣 憲一

  学術研究助成基金助成金／基盤研究(B), Apr. 2017 - Mar. 2020, Principal investigator

  Competitive research funding


• パターン化人工膜を用いた広範な膜タンパク質のラフト親和性と機能の解析

  森垣 憲一

  科学研究費補助金／基盤研究(B), Apr. 2017 - Mar. 2020, Principal investigator

  Competitive research funding


• ナノ空間に閉じ込めた人工膜の開発

  森垣 憲一

  学術研究助成基金助成金／挑戦的萌芽研究, Apr. 2015 - Mar. 2017, Principal investigator

  Competitive research funding


• パターン化人工膜を用いた膜タンパク質のラフト親和性と機能の解析

  森垣 憲一

  科学研究費補助金／基盤研究(B), Apr. 2014 - Mar. 2017, Principal investigator

  Competitive research funding


• Localized surface plasmon sensing for subtype determination of influenza viruses in a specimen with ultra-small volume

  KANO Hiroshi, HASEGAWA Hideki, MORIGAKI Kenichi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Muroran Institute of Technology, 01 Apr. 2013 - 31 Mar. 2016

  Development of a method for quick subtype determination of influenza viruses is important because infectious and pathogenic properties of viruses are different. In this research, we proposed a method in which refractive index change due to antigen-antibody reaction between target viruses with certain subtype and monoclonal antibodies fixed in a micro-channel on a sensing substrate. To realize this concept, we successfully improved stability and sensitivity of a measurement system for virus detection, confirmed a measurement principle for faster data acquisition, and developed a technique to modify chemical property of a substrate surface to locate certain antibodies in a controlled region.


• 脂質膜上でのアミロイド凝集と膜動態に及ぼす影響

  笹原 健二

  科学研究費補助金／基盤研究(C), Apr. 2012 - Mar. 2015

  Competitive research funding


• Localized surface plasmon sensing for detection and diagnosis of influenza viruses

  KANO Hiroshi, HASEGAWA Hideki, MORIGAKI Kenichi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Muroran Institute of Technology, 2010 - 2012

  A method to determine subtype of influenza viruses by using surface plasmons localized in microscopic region on a flat metal surface was developed. In this method, refractive index variation arisen from interactions between viruses and their monoclonal antibodies was measured. Theoretical calculation revealed that measurement region had a size of ～180nm in radius, and could be occupied by ～7 viruses. The detection of influenza viruses with certain subtype was successfully demonstrated.


• パターン化モデル生体膜と微小構造体を接合したナノ界面における分子認識と分子輸送

  森垣 憲一

  科学研究費補助金／新学術領域研究, 2011, Principal investigator

  Competitive research funding


• パターン化脂質二分子膜を利用したタンパク質の濃縮と２次元結晶化

  森垣 憲一

  科学研究費補助金／挑戦的萌芽研究, 2009, Principal investigator

  Competitive research funding


• パターン化モデル生体膜を利用した膜タンパク質再構成技術の開発

  森垣 憲一

  科学研究費補助金／特定領域研究, 2009, Principal investigator

  Competitive research funding


• Development of integrated model biological membranes on solid substrates

  MORIGAKI Kenichi, KUBO Tai

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), National Institute of Advanced Industrial Science and Technology, 2006 - 2007

  Micropatterned phospholipid bilayers on solid substrates offer an attractive platform for various applications, such as high throughput drug screening. We are currently developing a method for generating micropatterned bilayers composed of polymerized and fluid lipid bilayers. Lithographic photopolymerization of a diacetylene-containing phospholipid (DiynePC) allowed facile fabrication of compartmentalized arrays of fluid lipid membranes. In the present project, we could find a key experimental parameter, that significantly influenced the homogeneity and quality of the fabricated polymeric bilayers, namely the temperature at which monolayers of monomeric DiynePC were formed on the water surface and transferred onto solid substrates by the Langmuir-Blodgett/Langmuir-Schaefer (LB/LS) technique. Using the fluorescence microscopy and atomic force microscopy, it was found that polymerized bilayers were homogeneous, if bilayers of DiynePC were prepared below the triple point temperature (ca. 20℃) of the monolayer, where a direct transition from the gaseous state to the liquid condensed state occurred. The differences were attributed to the domain structures in the monolayer that was transferred from the water surface to the substrate. Another important result from the project is the development of a method to incorporate lipid membranes with membrane proteins. We used mixtures of short and long chain phospholipid, 1, 2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). We demonstrated that formation of planar bilayers was significantly accelerated in the presence of DHPC. Furthermore, membranes containing calcium channels(SR membranes from rabbit muscle) could be incorporated in the model membrane. These results suggest that the use of the mixtures of short and long chain phospholipids could be a convenient means for preparing integrated model membranes.

• ナノギャップ構造型基板

  MORIGAKI KENICHI

  特願2015-001194, 16 Jan. 2015, 大学長, 特許6400483, 14 Sep. 2018, Domestic

  Patent right


• シトクロムP450酵素代謝活性パターンによる化合物の毒性予測法

  森垣 憲一, 堀本 勝久, 今石 浩正

  特願2012-023269, 06 Feb. 2012, 大学長, 特許5930170, 13 May 2016, Domestic

  Patent right


• 多様なチトクロムＰ４５０分子種の酵素活性を網羅的かつ高効率で測定する方法及びキット

  今石 浩正, 森垣 憲一, 常 鋼, 達 吉郎

  特願2011-529888, 27 Aug. 2010, 大学長, WO11027718, 10 Mar. 2011, 特許5713318, 20 Mar. 2015

  Patent right


• ポリマー脂質二分子膜を用いた機能性基板

  森垣 憲一, 岡崎 敬, 達 吉郎, 中島 芳浩

  特願2010-097027, 20 Apr. 2010, 特開2011-226920, 2011, 特許5532229, 09 May 2014, Domestic

  Patent right


• ベシクル融合法による光重合性脂質二分子膜の作製方法

  森垣 憲一, 岡崎 敬

  特願2009-004265, 13 Jan. 2009, 特開2010-163471, 29 Jul. 2010, Domestic

  Patent right


• 固定化チトクロムＰ４５０と酸素センサーを有する積層基板

  森垣 憲一, 常 鋼, 今石 浩正

  特願2009-201190, 01 Sep. 2009, 大学長, Domestic

  Patent right


• ＮＡＤＰＨ依存性酵素又は該依存性酵素により還元される酸化酵素の酵素活性を測定する方法及びキット

  森垣 憲一, 達 吉郎, 今石 浩正

  特願2009-201187, 01 Sep. 2009, 大学長, Domestic

  Patent right


• 基板上に固定化された膜結合型チトクローム P450の活性測定

  今石 浩正, 森垣 憲一, 達 吉郎, 湯元 昇

  特願2007-027382, 06 Feb. 2007, 大学長, 特開2008-187975, 21 Aug. 2008, Domestic

  Patent right


• 光重合性脂質膜による膜内分子の水平拡散制御

  森垣 憲一

  特願2004-069864, 12 Mar. 2004, 特開2004-309464, 04 Nov. 2004, Domestic

  Patent right