Research activity information

• Jul. 2025 The 11th EAFES International Congresas, Grand Prize, Relationship between anurans density and landscape factors in paddy field using environmental DNA analysis

  Akinori Ogura, Qianqian WU, Ryohei NAKAO, Atushi USHIMARU, Toshifumi, MINAMOTO


• Dec. 2024 日本環境DNA学会, 優秀賞: 日本環境DNA学会第7回環境DNA学会つくば大会, 琵琶湖におけるブラックバス類の時空間的分布動態の解明

  岡田淳史, 脇村圭, 内井喜美子, 鄔倩倩, 源利文


• Nov. 2022 日本環境DNA学会「あなたが主役のワークショップ」, 優秀賞, 環境DNAメタバーコーディングによる深海無脊椎動物 の検出。

  鄔倩倩, 西澤崚平, 藤原義弘, 土田真二, 吉田尊雄, 河戸勝, 駒井智幸, 源利文

• Climate Change Impacts on Antarctic Fish Diversity: A Circumpolar Perspective From eDNA Metabarcoding Assessment

  Hai Li, Fang Yang, Yuzhuo Liao, Xiang Zhang, Yuan Li, Rui Wang, Ran Zhang, Dong Li, Qianqian Wu, Longshan Lin

  ABSTRACT Aim Recent climate‐driven changes in the Antarctic marine environment, marked by regional differences between West and East Antarctica, may alter ecosystem dynamics. As key components of the food web, fish might reflect these changes. This study examines fish biodiversity patterns across four typical Antarctic seas by integrating eDNA analysis with bottom trawl surveys, focusing on biodiversity shifts and range alterations in response to varying levels of climate change exposure. Location Four ecologically distinct Antarctic regions: The Adjacent Waters of Antarctic Peninsula (AAP), the Amundsen Sea‐Bellingshausen Sea (ASBS), the Prydz Bay‐Cooperation Sea (PBCS), and the Cosmonaut Sea (CS). Methods During the 37th Chinese National Antarctic Research Expedition, we integrated eDNA metabarcoding (from both pelagic and benthic water layers) with bottom trawl surveys across four regions (AAP, ASBS, PBCS, and CS) to characterise regional fish community. Results Environmental DNA metabarcoding and trawl surveys detected 40 and 27 Antarctic fish species, respectively. Environmental DNA proved effective in complementing traditional methods and capturing early life stages, especially with a refined local reference database. Species belonging to the suborder Notothenioidei dominated across all surveyed regions. Ten species exhibited range extensions beyond previously documented distributions, likely reflecting historical data gaps, with the exception of Patagonotothen sp. Main Conclusions eDNA metabarcoding showed potential for monitoring Antarctic ichthyoplankton. Fish diversity in the four studied seas aligns with the general pattern in the Southern Ocean, while ASBS was identified as a previously overlooked fish diversity hotspot. The southward detection of Patagonotothen sp. may signal climate‐driven shifts in species distribution. Future research integrating eDNA with interdisciplinary approaches holds promise for advancing the monitoring of climate change impacts on Antarctic marine ecosystems.

  Corresponding, Wiley, Jul. 2025, Diversity and Distributions, 31(7) (7)

  [Refereed]

  Scientific journal


• Development of universal PCR primers for the environmental DNA metabarcoding of cephalopod (Mollueca) diversity

  Qianqian Wu, Tomoyuki Nakano, So Ishida, Tomoyuki Komai, Yoshihiro Fujiwara, Takao Yoshida, Masaru Kawato, Shin-ichiro Oka, Katsunori Fujikura, Masaki Miya, Toshifumi Minamoto

  Elsevier BV, Mar. 2025, Marine Environmental Research, 208, 107094 - 107094, English

  Scientific journal


• Investigation on the Distribution of Bangana Tungting in Yuanshui Unique Fish Species National Aquatic Germplasm Resources Reserve Using Environmental DNA Technology

  Lu Tian, Qianqian Wu, Li Zou, Jinxin Zhou, Chongrui Wang, Linmei Han, Zaiquan Zhang, Xing Xiang, Mingqiu Liu, Zhifeng Feng, Zhonggui Xie, Zhiqiang Liang

  Corresponding, Dec. 2024, Ecology and Evolution

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• QuickConc: A rapid, efficient, and power-free eDNA concentration method with cationic-assisted capture

  Tomohiro Kuroita, Qianqian WU, Ryo Iwamoto, Toshifumi Minamoto

  Sep. 2024


• Risk Screening of Invasive Aquatic Species and a Survey of Fish Diversity Using Environmental DNA Metabarcoding Analysis in Shanghai

  Ruohan Yu, Qianqian Wu, Fan Li, Aibin Zhan, Jinxin Zhou, Shan Li

  Corresponding, Jan. 2024, Diversity

  [Refereed]

  Scientific journal


• Opposite trends in environmental DNA distributions of two freshwater species under climate change

  Qianqian Wu, Jinxin Zhou, Tatsuya Komoto, Toshiyuki Ishikawa, Naoshige Goto, Masayuki K. Sakata, Daisuke Kitazawa, Toshifumi Minamoto

  Lead, Sep. 2023, Ecosphere

  [Refereed]

  Scientific journal


• Improvement of recovery yield of macro-organismal environmental DNA from seawater samples

  Qianqian Wu, Toshifumi Minamoto

  May 2023, Analytical Sciences

  [Refereed]

  Scientific journal


• Estimating the spawning activity of fish species using nuclear and mitochondrial environmental DNA concentrations and their ratios

  Luhan Wu, Qianqian Wu, Takashi Inagawa, Jiro Okitsu, Shogo Sakamoto, Toshifumi Minamoto

  Abstract During spawning activity, fish release large amounts of sperm and eggs into the water, which has been assumed to cause an increase in environmental DNA (eDNA) levels and nuclear DNA/mitochondrial DNA ratios. To test whether these assumptions are valid and whether nuclear and mitochondrial eDNA analysis can be used to monitor the spawning activity of freshwater fish, we conducted field eDNA surveys and traditional surveys using common carp (Cyprinus carpio), largemouth bass (Micropterus salmoides) and bluegill sunfish (Lepomis macrochirus) as model species. Fish spawning periods were estimated based on age, as estimated using the body lengths of juveniles collected in the Miharu reservoir in Fukushima, Japan. The results showed that the main spawning periods of largemouth bass and bluegill sunfish were from April to July and from July to August, respectively. Field eDNA surveys were conducted in the Hebisawagawa front reservoir, which is connected to the Miharu reservoir. From March to August 2019 and 2020, weekly eDNA sampling was conducted at three sites, and daily sampling was conducted at six sites from 23 June to 3 July 2020. The eDNA concentrations of the nuclear internal transcribed spacer 1 (ITS1) and mitochondrial cytochrome B (CytB), as well as the ITS1/CytB ratio, were measured for each of the three fish in each water sample. Water temperature had a statistically significant effect on eDNA concentration, probably reflecting the relationship between water temperature and spawning. We created generalised additive mixed models to estimate spawning activity periods based on weekly eDNA data. The estimated periods of spawning activity for common carp, largemouth bass and bluegill sunfish were March to May, May to July, and May to August, respectively. The estimated spawning periods coincided with known fish ecology or the results of traditional methods. This method also has been applied to daily eDNA samples, showing the feasibility of high‐resolution estimation of spawning activity. For common carp and bluegill sunfish, we were able to estimate the spawning period using this method. Although the method is affected by biomass and the diffusion and degradation of eDNA, it has the potential to accurately estimating spawning activities. These then can be estimated without conducting laborious traditional surveys, facilitating the monitoring of reproduction by rare, invasive or important fishery species. Further research on the diffusion distance and degradation time of the eDNA concentration peak caused by fish spawning activity may improve the accuracy of monitoring.

  Wiley, Nov. 2022, Freshwater Biology, 68(1) (1), 103 - 114

  [Refereed]


• Application of environmental DNA metabarcoding in a lake with extensive algal blooms

  Qianqian Wu, Masayuki K. Sakata, Deyi Wu, Hiroki Yamanaka, Toshifumi Minamoto

  Aug. 2021, Limnology

  [Refereed]

  Scientific journal


• Hepatopancreas Transcriptome Profiling Analysis Reveals Physiological Responses to Acute Hypoxia and Reoxygenation in Juvenile Qingtian Paddy Field Carp Cyprinus carpio var qingtianensis

  Ming Qi, Qianqian Wu, Tao Liu, Yiling Hou, Yixin Miao, Menghong Hu, Qigen Liu

  Lead, Frontiers Media {SA}, Sep. 2020, Frontiers in Physiology, 11

  [Refereed]

  Scientific journal


• Habitat selection and migration of the common shrimp, Palaemon paucidens in Lake Biwa, Japan—An eDNA‐based study

  Qianqian Wu, Ken Kawano, Toshiyuki Ishikawa, Masayuki K. Sakata, Ryohei Nakao, Masayoshi K. Hiraiwa, Satsuki Tsuji, Hiroki Yamanaka, Toshifumi Minamoto

  Wiley, May 2019, Environmental DNA, 1(1) (1), 54 - 63

  [Refereed]

  Scientific journal


• The life history with seasonal migration of the lacustrine shrimp Palaemon paucidens in an ancient lake in Japan

  Qianqian Wu, Yasuoki Takami, Toshifumi Minamoto, Toshiyuki Ishikawa

  Wiley, Apr. 2019, Ecosphere, 10(4) (4), English

  [Refereed]

  Scientific journal


• Environmental DNA reveals nonmigratory individuals of Palaemon paucidens overwintering in Lake Biwa shallow waters

  Qianqian Wu, Ken Kawano, Yoshitoshi Uehara, Noboru Okuda, Masamichi Hongo, Satsuki Tsuji, Hiroki Yamanaka, Toshifumi Minamoto

  University of Chicago Press, Jun. 2018, Freshwater Science, 37(2) (2), 307 - 314, English

  [Refereed]

  Scientific journal

• 環境DNAを用いた陸上哺乳類と鳥類の検出方法の開発

  中村仁湖, 橋本渚, 坂田雅之, 邬倩倩, 源利文

  第6回環境DNA学会九州大会, Dec. 2023, Japanese

  Nominated symposium


• 環境DNA分析と生態モデルによる気候変動下における生物の時空間分布予測

  邬倩倩, 周金鑫, 河本達也, 石川俊之, 後藤直成, 坂田雅之, 北澤大輔, 源利文

  第6回環境DNA学会九州大会, Dec. 2023, Japanese

  Poster presentation

• 日本環境DNA学会

  Nov. 2018 - Present


• 日本生態学会

  Mar. 2016 - Present

• 環境DNA分析と生態モデルによる気候変動下における魚類の時空間分布予測

  鵜倩倩

  公益財団法人中辻創智社, 神戸大学人間発達環境学研究科, Jul. 2024 - Mar. 2026


• 環境DNAメタバーコーディングとQGISを活用した水生コウチュウ目の多様性解析

  鄔 倩倩

  関西の自然保護・自然環境保全に関する調査研究, 神戸大学, Apr. 2024 - Mar. 2025


• 琵琶湖産スジエビにおける集団間の生態的・遺伝的分化の解明

  鄔 倩倩

  関西の自然保護・自然環境保全に関する調査研究, 神戸大学, Apr. 2017 - Mar. 2018

• PCR amplification primers for detecting and identifying Ommastrephes bartrami species in northern Pacific Ocean through environmental DNA and detection method and applications of PCR amplification primers

  Xinjun, Chen, Q. Wu, Y. Liu, B. Liu, C. Wang

  特願LU102260, 02 Dec. 2020, 上海海洋大学, 特開LU102260, 02 Dec. 2021