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AZUMA Tetsushi
Graduate School of Agricultural Science / Department of Bioresource Science
Professor

Researcher basic information

■ Research Areas
  • Environmental science/Agricultural science / Crop production science

Research activity information

■ Paper
■ MISC
  • Responsiveness of Expression of Starch Synthesis-Related Genes to CRCT in Vegetative Organs of Rice
    宮川文宏, 森田隆太郎, 笹山大輔, 畠中知子, 東哲司, 深山浩
    2023, 日本作物学会講演会要旨集, 255th

  • Effect of gravity and light on growth direction in crown roots of floating rice
    TSUBOTA Hiroki, SASAYAMA Daisuke, ITOH Kazuyuki, AZUMA Tetsushi
    09 Sep. 2013, 日本作物學會紀事, 82, 342 - 243, Japanese

  • Low-oxygen-induced intemodal elongation in floating rice
    HARADA Shohei, ITO Shunsuke, SASAYAMA Daisuke, HIRANO Tatsuya, ITOH Kazuyuki, AZUMA Tetsushi
    09 Sep. 2013, 日本作物學會紀事, 82, 346 - 347, Japanese

  • Effect of gravity on elongation growth of rice
    ISHIHARA Ruriko, AZUMA Tetsushi, ITOH Kazuyuki
    09 Sep. 2012, 日本作物學會紀事, 81, 310 - 311, Japanese

  • Involvement of ethylene in the elongation responses to flood in Amazonian wild rice species, Oryza glumaepatula and O. grandiglumis
    OKISHIO Takuma, AZUMA Tetsushi, ITOH Kazuyuki
    09 Sep. 2012, 日本作物學會紀事, 81, 306 - 307, Japanese

  • Relationship between Energy Status and Gravitropic Response of Cerial Crops in Anoxia
    HAMADA Yuma, AZUMA Tetsushi, ITOH Kazuyuki
    09 Sep. 2012, 日本作物學會紀事, 81, 308 - 309, Japanese

  • Gaseous factors of regulating internode elongation during sprouting of tubers in Sagittaria trifolia L. and S. pygmaea Miq.
    SAKAI Hisateru, AZUMA Tetsushi, ITOH Kazuyuki
    09 Sep. 2012, 日本作物學會紀事, 81, 312 - 313, Japanese

  • Response to flash flood in South American wild rice species
    HIGAMI Toshiaki, OKISHIO Takuma, ITOH Kazuyuki, AZUMA Tetsushi
    30 Aug. 2011, 日本作物學會紀事, 80, 244 - 245, Japanese

  • Effect of Deposition of Phenolic Substances and Polysaccharides on Susceptibility to Expansins in the Cell Wall of Floating Rice Internodes
    OHTA Kensuke, SASAYAMA Daisuke, ITOH Kazuyuki, AZUMA Tetsushi
    30 Aug. 2011, 日本作物學會紀事, 80, 224 - 225, Japanese

  • Responces to a rapid rise in water level in the South American wild rice species Oryza grandiglumis and O. glumaepatula
    OKISHIO Takuma, AZUMA Tetsushi, ITOH Kazuyuki
    03 Sep. 2010, 日本作物學會紀事, 79, 252 - 253, Japanese

  • Comparison of Gravitropic Response of Shoots to Anoxia in Cereal Crops
    INOUE Yoshitaka, AZUMA Tetsushi, ITOH Kazuyuki
    03 Sep. 2010, 日本作物學會紀事, 79, 250 - 251, Japanese

  • Differences between Sagittaria trifolia and S. pygmaea in the properties of organs developing from tubers during emergence
    IKEMACHI Kenta, AZUMA Tetsushi, ITOH Kazuyuki
    03 Sep. 2010, 日本作物學會紀事, 79, 398 - 399, Japanese

  • Effect of submergence and irradiance on internodal elongation of floating rice
    AZUMA Tetsushi, SADAI Ayako, ITOH Kazuyuki
    27 Mar. 2009, 日本作物學會紀事, 78, 308 - 309, Japanese

  • Floating ability of Oryza grandiglumis and O. glumaepatula
    OKISHIO Takuma, AZUMA Tetsushi, ITOH Kazuyuki
    27 Mar. 2009, 日本作物學會紀事, 78, 304 - 305, Japanese

  • Effect of the Deposition of Ferulic and Diferulic Acids on the Susceptibility to Expansin Action in the Cell Walls of Floating Rice Internodes
    SASAYAMA Daisuke, AZUMA Tetsushi, ITOH Kazuyuki
    27 Mar. 2009, 日本作物學會紀事, 78, 300 - 301, Japanese

  • Growth in Internodes of Floating Rice: Hormonal Regulations and Cell Wall Modifications
    Tetsushi Azuma, Daisuke Sasayama, Kazuyuki Itoh
    Floating rice is the only crop that can be grown in flood plains because of the capacity to elongate internodes rapidly under conditions of flooding. The mechanisms of growth adaptation to flooding stress have been investigated from ecological, morphological, genetical, physiological, biochemical and molecular biological points of view. In this review, we mainly describe our r
    The Crop Science Society of Japan, Jan. 2009, Japanese Journal of Crop Science, 78(1) (1), 1 - 8, Japanese
    [Refereed]
    Introduction scientific journal

  • P-46 Promotion of Shoot Growth by a Low Oxygen Environment in Floating Rice
    Sadai Ayako, Azuma Tetsushi, Itoh Kazuyuki
    The Crop Science Society of Japan, 2008, Japanese Journal of Crop Science, 77(2) (2), 306 - 307, Japanese

  • P-47 Relationship between Cell Wall Composition and Responsiveness to Expansin in Floating Rice Internodes
    Sasayama Daisuke, Azuma Tetsushi, Itoh Kazuyuki
    The Crop Science Society of Japan, 2008, Japanese Journal of Crop Science, 77(2) (2), 308 - 309, Japanese

  • Gravitropic response of shoot in floating rice
    Takenaga Y., Sasayama D., Azuma T., Nanmori T., Yasuda T.
    Japanese Society for Tropical Agriculture, 25 Sep. 2003, Japanese journal of tropical agriculture, 47(2) (2), 29 - 30, Japanese

  • Internodal elongation and enhancement of transpiration by ethylene in floating rice
    AZUMA Tetsushi, HATANAKA Tomoko, UCHIDA Naotsugu, YASUDA Takeshi
    The Crop Science Society of Japan, 17 Sep. 2003, Japanese Journal of Crop Science, 72(2) (2), 304 - 305, Japanese

  • Changes in expansin activity and response of cell wall during internodal elongation in floating rice
    SASAYAMA Daisuke, AZUMA Tetsushi, NANMORI Takashi, YASUDA Takeshi
    The Crop Science Society of Japan, 17 Sep. 2003, Japanese Journal of Crop Science, 72(2) (2), 306 - 307, Japanese

  • Low levels of oxygen promote internodal elongation in floating rice independently of enhanced ethylene production
    AZUMA Tetsushi, UCHIDA Naotsugu, YASUDA Takeshi
    The Crop Science Society of Japan, 27 Sep. 2001, Japanese Journal of Crop Science, 70(2) (2), 193 - 194, Japanese

  • Changes in cell wall(1→3)(1→4)-β-D-glucan levels and glucanase activities in elongating internodes of floating rice
    MURANAGA Jun-ichiro, OKITA Naoko, AZUMA Tetsushi, NANMORI Takashi, YASUDA Takeshi
    The Crop Science Society of Japan, 2001, Japanese Journal of Crop Science, 70(2) (2), 195 - 196, Japanese

  • POSTULATION OF NEW TYPE AGC-PROTEIN KINASE IN BOTH PLANT & ANIMAL :
    FUJITA Naoya, MATSUOKA Daisuke, NANMORI Takashi, AZUMA Tetsushi, YASUDA Takeshi
    Japanese Society of Plant Physiologists, 2001, Plant and cell physiology, 42, s106, English

  • EFFECT OF OSMOLARITY, DEHYDRATION AND ABA ON AMOUNTS OF ATSRPK1 TRANSCRIPTS :
    KOHNO Tomoko, MATSUOKA Daisuke, NANMORI Takashi, AZUMA Tetsushi, YASUDA Takeshi
    Japanese Society of Plant Physiologists, 2001, Plant and cell physiology, 42, s93, English

  • Changes in the Rate of Catalytic Turnover and the Number of Carbamylated Site of Rubisco during Leaf Aging of Rice
    FUKAYAMA Hiroshi, UCHIDA Naotsugu, AZUMA Tetsushi, YASUDA Takeshi
    The Crop Science Society of Japan, 02 Apr. 1998, Japanese Journal of Crop Science, 67, 104 - 105, Japanese

  • 68 Photosynthetic Characteristics in Wild Rice Species : The in vivo Activity of Rubisco
    HIRAOKA M., UCHIDA N., FUKAYAMA H., AZUMA T., YASUDA T.
    野生イネO.barthiiとO.meridionalisのin vivoのRubisco比活性は栽培種のそれより有意に高く, Rubiscoの活性比率とRubisco activase含量も高い。
    The Crop Science Society of Japan, 18 Oct. 1997, Japanese Journal of Crop Science, 66(2) (2), 135 - 136, Japanese

  • 67 Relationship between Specific Degradation of Rubisco and Increased Proteolytic Activity in a Rice Leaf Piece Induced by ABA
    FUKAYAMA Hiroshi, ARAI Rie, UCHIDA Naotsugu, AZUMA Tetsushi, YASUDA Takeshi
    The Crop Science Society of Japan, 02 Apr. 1997, Japanese Journal of Crop Science, 66(1) (1), 134 - 135, Japanese

  • 66 Relaxation Time for Activation of CO_2 Exchange Decreases with Leaf Aging and It is Related to Rubisco activase/Rubisco Ratio
    FUKAYAMA Hiroshi, UCHIDA Naotsugu, AZUMA Tetsushi, YASUDA Takeshi
    The Crop Science Society of Japan, 02 Apr. 1997, Japanese Journal of Crop Science, 66(1) (1), 132 - 133, Japanese

  • ETHYLENE-INDUCED ELONGATION AND TRANSPIRATION IN INTERNODES OF FLOATING RICE
    AZUMA Tetsushi, HARA Ai, YAMAMURA Noriko, UCHIDA Naotsugu, YASUDA Takeshi
    Mar. 1997, Plant and cell physiology, 38, s116, English

  • 105 Gibberellin-induced elongation and osmoregulation in internodes of floating rice
    AZUMA Tetsushi, UENO Satoshi, UCHIDA Naotsugu, YASUDA Takeshi
    The Crop Science Society of Japan, 02 Oct. 1996, Japanese Journal of Crop Science, 65(2) (2), 209 - 210, Japanese

  • 88 Photosynthetic characteristics in wild rice species
    Hiraoka Motoaki, Fukayama Hiroshi, Uchida Naotsugu, Azuma Tetsushi, Yasuda Takeshi
    The Crop Science Society of Japan, 02 Oct. 1996, Japanese Journal of Crop Science, 65(2) (2), 175 - 176, Japanese

  • 54 Changes in translocation of photoassimilates and activities of sucrose synthesis enzymes in floating rice growing rapidly under submergence.
    HIRANO Tatsuya, UCHIDA Naotsugu, AZUMA Tetsushi, YASUDA Takeshi
    The Crop Science Society of Japan, 03 Apr. 1996, Japanese Journal of Crop Science, 65(1) (1), 108 - 109, Japanese

  • 42 Effects of Plant growth regulators on photosynthetic activity of senescing rice leaf slices : Rubisco activase, Rubisco and proteinase
    FUKAYAMA Hiroshi, UCHIDA Naotsugu, AZUMA Tetsushi, YASUDA Takeshi
    The Crop Science Society of Japan, 03 Apr. 1996, Japanese Journal of Crop Science, 65(1) (1), 84 - 85, Japanese

  • GIBBERELLIN-INDUCED ELONGATION AND OSMOREGULATION IN INTERNODES OF FLOATING RICE
    AZUMA Tetsushi, UENO Satoshi, UCHIDA Naotsugu, YASUDA Takeshi
    Mar. 1996, Plant and cell physiology, 37, 144 - 144, English

  • 48 Relation between photosynthetic activity and Rubisco activase content in rice leaves from emergence through senescence
    Fukayama Hiroshi, Uchida Naotsugu, Azuma Tetsushi, Yasuda Takeshi
    イネ葉の加齢に伴う光合成速度の変化はRubisco activase含量との関係が最も密接で, in vivoの活性はRubiscoに対するRubisco activaseの比率が高いほど高い.
    The Crop Science Society of Japan, 03 Apr. 1995, Japanese Journal of Crop Science, 64(1) (1), 96 - 97, Japanese

  • ETHYLENE AND GROWTH IN INTERNODES OFFLOATING RICE UNDER HYPOXIA
    AZUMA Tetsushi, NISHI Fumiko, UENO Satoshi, UCHIDA Naotsugu, YASUDA Takeshi
    Mar. 1995, Plant and cell physiology, 36, S84, English

  • OXYGEN AND GROWTH IN INTERNODES OFSUBMERGED FLOATING RICE
    AZUMA Tetsushi, KANEDA Yasushi, SUMIDA Yoriko, UCHIDA Naotsugu, YASUDA Takeshi
    Mar. 1995, Plant and cell physiology, 36, S84, English

  • ROLE OF ETHYLENE IN SOMATIC EMBRYOGENESISOF COFFEA CANEPHORA
    HATANAKA Tomoko, AZUMA Tetsushi, UCHIDA Naotsugu, YASUDA Takeshi
    Mar. 1995, Plant and cell physiology, 36, S109, English

  • Purification of Rubisco activase from rice leaves
    FUKAYAMA H., Uchida Naotsugu, Azuma Tetsushi, Yasuda Takeshi, Yamaguchi Tadashi
    ゲルろ過とイオン交換クロマトグラフィーにより, イネ葉より Rubisco activase を精製することができた. 12.5% SDS-PAGE の結果それは約40 kDa のペプチドであることがわかった.
    The Crop Science Society of Japan, 1994, Jpn. J. Crop Sci., 63(1) (1), 140 - 141, Japanese

■ Books And Other Publications
  • 熱帯農学概論
    AZUMA TETSUSHI
    Joint work, 培風館, Jan. 2019, Japanese, ISBN: 9784563078263
    Scholarly book

  • 作物栽培体系７ 工芸作物の栽培と利用
    AZUMA TETSUSHI
    Joint work, 朝倉書店, Jul. 2017, Japanese
    Scholarly book

  • 作物学
    AZUMA TETSUSHI
    Joint work, 文永堂出版, Nov. 2013, Japanese
    Scholarly book

  • 作物学用語事典
    AZUMA TETSUSHI
    Joint work, 農山漁村文化協会, Mar. 2010, Japanese
    Scholarly book

■ Lectures, oral presentations, etc.
  • 異なる窒素施与条件が根粒着生能の異なる3種の準同質系統ダイズの種子収量・品質に与える影響解析—完熟種子δ15N値に基づく窒素同化・固定量の評価
    濱口 秀生, 武田 旭永, 山本 直樹, 増村 威宏, 杉本 敏男, AZUMA TETSUSHI
    種子生理生化学研究会, Nov. 2018, Japanese, Domestic conference
    Oral presentation

  • Vernonia galamensis DGAT1 can increase Arabidopsis seed oil level more than other DGAT1s
    HATANAKA TOMOKO, 冨田 典希, 周 采穎, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI, DAVID F. HILDEBRAND
    第31回植物脂質シンポジウム, Nov. 2018, Japanese, 高知大学, Domestic conference
    Poster presentation

  • Vernonia galamensis DGAT1 can increase Arabidopsis seed oil content more than other DGAT1s
    HATANAKA TOMOKO, YOSHIKI TOMITA, CHOI-WING CHAU, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI, DAVID F. HILDEBRAND
    The 14th International Symposium on Biocatalysis and Agricultural Biotechnology, Oct. 2018, English, Kuala Lumpur, Malaysia, International conference
    Poster presentation

  • Analysis of Floating Response of South American Wild Rice Oryza glumaepatula
    NIIKAWA MAYUKO, ENDO HIROAKI, SASAYAMA DAISUKE, HATANAKA TOMOKO, FUKAYAMA HIROSHI, AZUMA TETSUSHI
    日本作物学会第246回講演会, Sep. 2018, Japanese, 北海道大学農学部, Domestic conference
    Poster presentation

  • 異なる窒素施与条件が根粒着生能の異なる3種の準同質系統ダイズの種子収量・品質に与える影響解析—完熟種子δ15N値に基づく窒素同化・固定量の評価
    濱口 秀生, 武田 旭永, 山本 直樹, 増村 威宏, 杉本 敏男, AZUMA TETSUSHI
    日本土壌肥料学会2018年度大会, Sep. 2018, Japanese, Domestic conference
    Oral presentation

  • Structure-Function Relationship of Hybrid Rubisco Consisting of Sorghum RbcS and Rice RbcL
    SHIOMI KEITA, SASAYAMA DAISUKE, HATANAKA TOMOKO, AZUMA TETSUSHI, YOSHIKAWA HIROKI, MATSUMURA HIROYOSHI, FUKAYAMA HIROSHI
    日本作物学会第246回講演会, Sep. 2018, Japanese, 北海道大学農学部, Domestic conference
    Oral presentation

  • Growth response of the African cultivated rice Oryza glaberrima and its wild ancestor Oryza barthii to flooding
    Luo Quanshu, NAKAZAWA MISAKI, SASAYAMA DAISUKE, HATANAKA TOMOKO, FUKAYAMA HIROSHI, AZUMA TETSUSHI
    日本作物学会第246回講演会, Sep. 2018, Japanese, 北海道大学農学部, Domestic conference
    Poster presentation

  • Highly active Vernonia galamensis DGAT1 can effectively increase oil levels in yeast, soybean and Arabidopsis
    HATANAKA TOMOKO, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI, MATSUOKA DAISUKE, TOMITA YOSHIKI, CHAU CHOI-WING, MIYASHITA WAKANA, SERSON WILLIAM, LI RUNZHI, ARMSTRONG PAUL, YU KESHUN, PFEIFFER TODD, LI XI-LE, HILDEBRAND DAVID
    The 23rd International Symposium on Plant Lipids, Jul. 2018, English, Yokohama, Japan, International conference
    Poster presentation

  • 根粒超着生系統ダイズの低収量の主因は、根粒自身ではない―根粒着生能の異なる三種の同質系統 ダイズへの窒素施与の影響比較―
    濱口 秀生, 武田 旭永, 山本 直樹, 増村 威宏, 杉本 敏男, AZUMA TETSUSHI
    近畿作物・育種研究会 第185回, Jun. 2018, Japanese, Domestic conference
    Oral presentation

  • アフリカイネ Oryza glaberrima とその祖先種 O. barthii の洪水に対する応答
    羅 全淑, 中澤 美沙希, SASAYAMA DAISUKE, HATANAKA TOMOKO, FUKAYAMA HIROSHI, AZUMA TETSUSHI
    近畿作物・育種研究会 第185回例会, Jun. 2018, Japanese, Domestic conference
    Oral presentation

  • シロイヌナズナにおける CRCT オーソログの機能解析
    萩原輝, 中馬望, MATSUOKA DAISUKE, SASAYAMA DAISUKE, HATANAKA TOMOKO, AZUMA TETSUSHI, FUKAYAMA HIROSHI
    日本作物学会講演会, 2018, Japanese, Domestic conference
    Poster presentation

  • イネの Rubisco activase 様タンパク質 Rca2 は Rubisco activase の機能を阻害する
    水本晃那, 小林亜希子, 山内靖雄, MATSUOKA DAISUKE, SASAYAMA DAISUKE, HATANAKA TOMOKO, AZUMA TETSUSHI, FUKAYAMA HIROSHI
    日本作物学会講演会, 2018, Japanese, Domestic conference
    Poster presentation

  • イネRubisco activase様タンパク質OsRca2はRubiscoの活性化を阻害する
    水本晃那, 小林亜希子, MATSUOKA DAISUKE, YAMAUCHI YASUO, SASAYAMA DAISUKE, HATANAKA TOMOKO, AZUMA TETSUSHI, FUKAYAMA HIROSHI
    日本光合成学会講演会, 2018, Japanese, Domestic conference
    Poster presentation

  • Functional analysis of Rubisco small subunit, OsRbcS1, that is not expressed in photosynthetic organs in rice
    小林 則之, 森田 耕一, SASAYAMA DAISUKE, HATANAKA TOMOKO, AZUMA TETSUSHI, FUKAYAMA HIROSHI
    International Symposium on Photosynthesis and Chloroplast Biogenesis, 2018, English, Domestic conference
    Poster presentation

  • C4植物の Rubisco 小サブユニットを高発現する形質転換イネの Rubisco 酵素特性
    小原崇, SASAYAMA DAISUKE, HATANAKA TOMOKO, AZUMA TETSUSHI, FUKAYAMA HIROSHI
    日本作物学会講演会, 2018, Japanese, Domestic conference
    Poster presentation

  • Highly active Vernonia galamensis DGAT1 can effectively increase seed oil levels in soybean and Arabidopsis
    HATANAKA TOMOKO, TOMITA YOSHIKI, Chau, Choi-Wing, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI, William Serson, Runzhi Li, Paul Armstrong, Keshun Yu, Todd Pfeiffer, Xi-Le Li, David F. Hildebrand
    The 7th Asian Symposium on Plant Lipid, Nov. 2017, English, Taipei, Taiwan, International conference
    Poster presentation

  • 浮稲性遺伝子と冠水耐性遺伝子を併せ持つイネ品種の深水成長応答と関連遺伝子の発現
    尾江 俊祐, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, HATANAKA TOMOKO, AZUMA TETSUSHI
    日本作物学会第244回講演会, Sep. 2017, Japanese, 岐阜大学, Domestic conference
    Poster presentation

  • 南米野生イネOryza glumaepatulaの深水に対する応答とSNORKEL遺伝子の多様性
    遠藤 宏朗, 新川 麻由子, SASAYAMA DAISUKE, HATANAKA TOMOKO, FUKAYAMA HIROSHI, AZUMA TETSUSHI
    日本作物学会第244回講演会, Sep. 2017, Japanese, 岐阜大学, Domestic conference
    Poster presentation

  • 深水環境下において浮稲節間の伸長を誘導する新規因子の探索
    冨田 一嘉, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, HATANAKA TOMOKO, AZUMA TETSUSHI
    日本作物学会第244回講演会, Sep. 2017, Japanese, 岐阜大学, Domestic conference
    Poster presentation

  • Does Vernonia DGAT1 show a higher activity than other DGAT1s in Arabidopsis seed?
    HATANAKA TOMOKO, TOMITA YOSHIKI, CHAU CHOI-WING, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI, David F. Hildebrand
    日本作物学会 第244回講演会, Sep. 2017, Japanese, 岐阜大学, Domestic conference
    Poster presentation

  • シロイヌナズナにおけるベルノニアDGAT1の働き
    HATANAKA TOMOKO, TOMITA YOSHIKI, CHAU CHOI-WING, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI, David F. Hildebrand
    第30回植物脂質シンポジウム, Sep. 2017, Japanese, お茶の水大学, Domestic conference
    Poster presentation

  • Vernonia DGAT1 can increase Arabidopsis seed oil content more than other DGAT1s
    HATANAKA TOMOKO, TOMITA YSHIKI, CHAU CHOI-WING, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI, HILDEBRAND, D. F
    Plant Biology 2017, Jun. 2017, English, Honolulu, Hawaii, USA, Plant oils represent one of the world’s most important renewable resources. In addition to extensive food uses, vegetable oils also have value as renewable chemical derivatives and fuels, such as biodiesel. Worldwide production of plant oil has been increasing. Triacylglycerols (TAGs) are the major component of plant storage lipid. Acyl-CoA:diacylglycerol acyltransferase (DGAT), International conference
    Poster presentation

  • マイクロアレイ法によるOsRbcS1発現抑制イネの遺伝子発現プロファイリング
    森田 耕一, SASAYAMA DAISUKE, AZUMA TETSUSHI, FUKAYAMA HIROSHI
    日本作物学会講演会, 2017, Japanese, Domestic conference
    Poster presentation

  • ソルガム高活性型Rubiscoの小サブユニットを利用したイネの光合成能力の改良
    塩見慶太, 山本滉樹人, HATANAKA TOMOKO, SASAYAMA DAISUKE, AZUMA TETSUSHI, FUKAYAMA HIROSHI
    農芸化学会関西支部会, 2017, Japanese, Domestic conference
    Oral presentation

  • イネにおけるRubisco activase様タンパク質OsRca2の機能解析
    水本晃那, 小林亜希子, HATANAKA TOMOKO, SASAYAMA DAISUKE, AZUMA TETSUSHI, FUKAYAMA HIROSHI
    農芸化学会関西支部会, 2017, Japanese, Domestic conference
    Oral presentation

  • 浮稲性遺伝子と冠水耐性遺伝子を併せ持つイネ系統の深水伸長応答
    OE SHUNSUKE, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI
    日本作物学会 第242回講演会, Sep. 2016, Japanese, 龍谷大学瀬田キャンパス, Domestic conference
    Poster presentation

  • 水田雑草オモダカとウリカワの塊茎萌芽期の節間伸長の酸素欠乏および二酸化炭素による促進
    SASAYAMA DAISUKE, FUKAYAMA HIROSHI, ITOH KAZUYUKI, AZUMA TETSUSHI
    日本作物学会 第242回講演会, Sep. 2016, Japanese, 龍谷大学瀬田キャンパス, Domestic conference
    Oral presentation

  • エチレンとジベレリンが誘導する浮稲節間の伸長促進に及ぼす重力方向と過重力の影響
    NAKATANI YUMI, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI
    日本作物学会 第242回講演会, Sep. 2016, Japanese, 龍谷大学瀬田キャンパス, Domestic conference
    Poster presentation

  • 浮稲性遺伝子と冠水耐性遺伝子を併せ持つイネ系統の生理特性
    OE SHUNSUKE, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI
    近畿作物・育種研究会 第181回例会, May 2016, Japanese, Domestic conference
    Oral presentation

  • 多年生水田雑草オモダカとウリカワの節間伸長はそれぞれ酸素欠乏と二酸化炭素によって促進される
    SASAYAMA DAISUKE, FUKAYAMA HIROSHI, ITOH KAZUYUKI, AZUMA TETSUSHI
    近畿作物・育種研究会 第181回例会, May 2016, Japanese, 滋賀県立大学 サテライト教室, Domestic conference
    Oral presentation

  • 野生イネOryza altaおよびO. latifoliaの洪水適応
    平井 雄基, SASAYAMA DAISUKE, AZUMA TETSUSHI
    日本作物学会 第240回講演会, Sep. 2015, Japanese, 信州大学長野 (工学) キャンパス, Domestic conference
    Poster presentation

  • 浮稲における冠水耐性遺伝子SUB1Aの存在とその機能
    岩見 裕太, SASAYAMA DAISUKE, AZUMA TETSUSHI
    日本作物学会 第240回講演会, Sep. 2015, Japanese, 信州大学長野 (工学) キャンパス, Domestic conference
    Oral presentation

  • アマゾン野生イネOryza glumaepatulaの深水適応
    SASAYAMA DAISUKE, 平井 雄基, AZUMA TETSUSHI
    日本作物学会 第240回講演会, Sep. 2015, Japanese, 信州大学長野 (工学) キャンパス, Domestic conference
    Oral presentation

  • アマゾン野生イネOryza glumaepatulaの浮稲性について
    SASAYAMA DAISUKE, AZUMA TETSUSHI
    近畿作物・育種研究会 第179回例会, May 2015, Japanese, 奈良県立橿原考古学研究所, Domestic conference
    Oral presentation

  • 浮稲茎葉部における重力屈性反応へのエクスパンシンの関与
    田口 あみ, SASAYAMA DAISUKE, ITOH KAZUYUKI, AZUMA TETSUSHI
    日本作物学会 第238回講演会, Sep. 2014, Japanese, 愛媛大学 城北キャンパス, Domestic conference
    Poster presentation

  • 浮稲不定根の成長方向と光の関係
    坪田 裕樹, SASAYAMA DAISUKE, ITOH KAZUYUKI, AZUMA TETSUSHI
    近畿作物・育種研究会 第177回例会, Jul. 2014, Japanese, 神戸大学農学部, Domestic conference
    Oral presentation

  • 浮稲茎葉部の重力屈性におけるエクスパンシンの関与
    田口 あみ, SASAYAMA DAISUKE, ITOH KAZUYUKI, AZUMA TETSUSHI
    近畿作物・育種研究会 第177回例会, Jul. 2014, Japanese, 神戸大学農学部, Domestic conference
    Oral presentation

  • 浮稲におけるSUB1A遺伝子の存在とその分類
    岩見 裕太, SASAYAMA DAISUKE, ITOH KAZUYUKI, AZUMA TETSUSHI
    近畿作物・育種研究会 第177回例会, Jul. 2014, Japanese, 神戸大学農学部, Domestic conference
    Oral presentation

  • アマゾン野生イネOryza grandiglumisの浮稲性と冠水耐性
    置塩 琢磨, SASAYAMA DAISUKE, 平野 達也, 秋本 正博, ITOH KAZUYUKI, AZUMA TETSUSHI
    近畿作物・育種研究会 第177回例会, Jul. 2014, Japanese, 神戸大学農学部, Domestic conference
    Oral presentation

  • Tuber sprouting and emergence of Sagittaria trifolia and S. pygmaea: their physiological traits and regulatory mechanisms
    笹山 大輔, Kenta Ikemachi, Hisateru Sakai, 伊藤 一幸, 東 哲司
    11th Conference of the International Society for Plant Anaerobiosis, Oct. 2013, English, IRRI, Los Baños, Laguna, Philippines, Emergent plants Sagittaria trifolia and S. pygmaea are perennial Alismataceous weeds of paddy field. Those plants mainly propagate vegetatively by tubers. Their sprouting from tubers starts with irrigation for paddling of the rice fields and continues for several months. Sprouting finally leads to emergence of the shoot parts of those plants from the soil. In this study, we cha, International conference
    Poster presentation

  • Effect of gravity and light on growth direction in crown roots of floating rice
    坪田 裕樹, 笹山 大輔, 伊藤 一幸, 東 哲司
    日本作物学会 第236回講演会, Sep. 2013, Japanese, 鹿児島大学 郡元キャンパス, Domestic conference
    Poster presentation

  • 低酸素環境が誘導する浮稲節間の伸長反応
    原田 翔平, 伊藤 俊輔, 笹山 大輔, 平野 達也, 伊藤 一幸, 東 哲司
    日本作物学会 第236回講演会, Sep. 2013, Japanese, 鹿児島大学 郡元キャンパス, Domestic conference
    Poster presentation

  • 浮稲節間の伸長に及ぼす重力の影響とオーキシンの関与
    石原 瑠璃子, SASAYAMA DAISUKE, ITOH KAZUYUKI, AZUMA TETSUSHI
    近畿作物・育種研究会 第175回例会, Jul. 2013, Japanese, 近畿大学生物理工学部, Domestic conference
    Oral presentation

  • イネ葉枕の重力屈性は嫌気環境で促進されるがコムギとエンバク葉枕では阻害される
    AZUMA TETSUSHI, 井上 義隆, 濱田 友馬, SASAYAMA DAISUKE, ITOH KAZUYUKI
    近畿作物・育種研究会 第175回例会, Jul. 2013, Japanese, 近畿大学生物理工学部, Domestic conference
    Oral presentation

  • 浮稲節間細胞壁におけるエクスパンシンの反応性に及ぼすフェノール化合物と多糖の影響
    太田 健介, SASAYAMA DAISUKE, ITOH KAZUYUKI, AZUMA TETSUSHI
    日本作物学会 第232回講演会, Sep. 2011, Japanese, 山口大学農学部, Domestic conference
    Poster presentation

  • 浮稲節間におけるエクスパンシンに対する細胞壁の反応性と壁構造との関係
    SASAYAMA DAISUKE, AZUMA TETSUSHI, ITOH KAZUYUKI
    日本作物学会 第226回講演会, Sep. 2008, Japanese, 神戸大学農学部, Domestic conference
    Poster presentation

  • 山口県の水田から見つかったスルホニルウレア抵抗性ミズマツバとアブノメ
    ITOH KAZUYUKI, 松本 晃, SASAYAMA DAISUKE, AZUMA TETSUSHI, 池尻 明彦, 平田 道弥
    日本作物学会 第226回講演会, Sep. 2008, Japanese, 神戸大学農学部, Domestic conference
    Oral presentation

  • 浮稲の深水条件下での節間伸長におけるエクスパンシンの関与
    SASAYAMA DAISUKE, AZUMA TETSUSHI, ITOH KAZUYUKI
    種生物学会 第39回種生物シンポジウム, Nov. 2007, Japanese, 六甲山YMCA, Domestic conference
    Poster presentation

  • Involvement of expansins in internodal elongation of submerged floating rice
    笹山 大輔, Tetsushi Azuma, Kazuyuki Itoh
    9th Conference of the International Society for Plant Anaerobiosis, Nov. 2007, English, Matushima, Sendai, Japan, International conference
    Poster presentation

  • 浮稲節間の細胞壁におけるエクスパンシンに対する反応性とフェノール性化合物の沈着の関係
    SASAYAMA DAISUKE, AZUMA TETSUSHI, ITOH KAZUYUKI
    日本作物学会 第224回講演会, Sep. 2007, Japanese, 金沢大学角間キャンパス, Domestic conference
    Oral presentation

  • 浮稲節間の伸長停止に伴うエクスパンシンの活性と細胞壁反応性の変化
    SASAYAMA DAISUKE, AZUMA TETSUSHI, NANMORI TAKASHI, 安田 武司
    日本作物学会 第221回講演会, Mar. 2006, Japanese, 東京大学農学部, Domestic conference
    Oral presentation

  • 浮稲節間組織における酸が誘導する成長とエクスパンシンとの関係
    SASAYAMA DAISUKE, AZUMA TETSUSHI, NANMORI TAKASHI, 安田 武司
    近畿作物・育種研究会 第159回例会, Jul. 2005, Japanese, 神戸大学農学部, Domestic conference
    Oral presentation

  • Identification of Substrate Specificity and Downstream Events of Arabidopsis AtMEKK1
    Takashi Nanmori, Toto Hadiart, Azuma Tetsusi, Takeshi Yasuda
    46th anual meeting of Japanese plant physiologists, Mar. 2005, English, nigata, Domestic conference
    Poster presentation

  • 塩ストレス下の塩生植物Atriplex nummulariaにおけるS-adenosylmethionine synthetaseの発現誘導
    多淵 知樹, 川口 祐介, 東 哲司, 南森 隆司, 安田 武司
    日本植物生理学会2004年度年会, 2004, Japanese, 日本植物生理学会, 東京都立大学, Domestic conference
    Oral presentation

  • アカザ科の植物のグリシンベタイン合成に関わる酵素の転写誘導に関する研究
    多淵 知樹, 井上 堅太郎, 佐々木 正浩, 東 哲司, 南森 隆司, 安田 武司
    日本植物生理学会2004年度年会, 2004, Japanese, 日本植物生理学会, 東京都立大学, Domestic conference
    Oral presentation

  • 浮稲節間の伸長に伴うエクスパンシンの活性と細胞壁反応性の変化
    SASAYAMA DAISUKE, AZUMA TETSUSHI, NANMORI TAKASHI, 安田 武司
    日本作物学会 第216回講演会, Sep. 2003, Japanese, 島根大学生物資源科学部, Domestic conference
    Oral presentation

  • 浮稲茎葉部の重力屈性反応について
    武長 裕子, SASAYAMA DAISUKE, AZUMA TETSUSHI, NANMORI TAKASHI, 安田 武司
    日本熱帯農業学会 第94回講演会, Sep. 2003, Japanese, 佐賀大学農学部, Domestic conference
    Oral presentation

  • 浮稲節間の伸長に伴うエクスパンシンの活性と細胞壁反応性の変化
    笹山 大輔, 東 哲司, 南森 隆司, 安田 武司
    日本作物学会記事, 2003, Japanese, 未記入, 未記入, Domestic conference
    Oral presentation

  • 浮稲節間の伸長とエチレンによる蒸散の促進
    東 哲司, 畠中 知子, 内田 直次, 安田 武司
    日本作物学会記事, 2003, Japanese, 未記入, 未記入, Domestic conference
    Oral presentation

  • 浮稲茎葉部の重力屈性反応について
    武長 裕子, 笹山 大輔, 東 哲司, 南森 隆司, 安田 武司
    熱帯農業, 2003, Japanese, 日本熱帯農業学会, 未記入, Domestic conference
    Oral presentation

■ Research Themes
  • 高温前処理によるイネメソコチル伸長の制御メカニズムの解明と栽培への応用
    東 哲司, 笹山 大輔, 平野 達也
    日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, Apr. 2021 - Mar. 2025
    高温前処理によるメソコチル伸長誘導とジベレリン (GA) の関連について調査を行った。高温前処理によるメソコチル伸長誘導が見られない矮性品種 (短銀坊主) にGAを投与してもメソコチル伸長は見られないが，高温前処理後にGAを投与するとメソコチル伸長誘導がみられたことから，高温前処理によるメソコチル伸長誘導には一定量のGAが不可欠であると推察した。しかし，非矮性品種にGA生合成阻害剤パクロプトラゾールを処理することで，高温前処理しなかった植物体ではメソコチル伸長が有意に抑制されたのに対して，高温前処理を行った植物体ではメソコチル伸長の抑制はわずかであった。GA関連遺伝子の発現解析したところ，SD1，OsGA3ox2，OsGA2ox3 のGA生合成遺伝子は高温前処理により発現が増大し，GA不活性化関連遺伝子の中には高温前処理区で発現量が低下するものがあった。これらの結果より，高温前処理によるメソコチル伸長誘導には一定量のGAが不可欠であるが，GAの関与のみでは説明できず他の因子が関与する可能性が考えられた。 そこでエチレンとジャスモン酸の関与について調べた。エチレン反応阻害剤で処理することにより，高温前処理によるメソコチル伸長誘導効果の減少が見られた。また，ジャスモン酸生合成関連遺伝子の GAOGAO1 の発現は，日本型品種のメソコチルが短い品種で高発現していたが，高温前処理により発現が低下することがわかった。 高温前処理の実用性について調査した。高温前処理した種子を30℃と4℃で保存したところ少なくとも4ヶ月間はメソコチルの伸長誘導効果は持続し，保存温度による違いは見られなかった。高温前処理は畑状態での深播きに有効であることは以前に示したが，湛水直播においても品種によっては有効であることがわかった。

  • 東 哲司
    学術研究助成基金助成金／基盤研究(C), Apr. 2018 - Mar. 2021, Principal investigator
    Competitive research funding

  • 東 哲司
    科学研究費補助金／基盤研究(C), Apr. 2012 - Mar. 2015, Principal investigator
    Competitive research funding

  • 伊藤 一幸
    学術研究助成基金助成金／挑戦的萌芽研究, Apr. 2013 - Mar. 2014
    Competitive research funding

  • Analysis of high photosynthetic function and high-yielding ability of backcross lines derived from Oryza sativa and wild rice species, O.rufipogon
    UCHIDA Naotsugu, SUGIMOTO Toshio, ISHII Takashige, AZUMA Tetsushi
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 2001 - 2004
    The present study was carried out to get some information for using wild rice species to improve leaf photosynthesis and grain yield. We examined photosynthetic rates and properties of dry matter production in BC2F1-BC2F4 lines. In BC2F1 populations, several lines had significantly higher photosynthetic rates (OER) than parental cultivars, and 14-25% of plants in the populations had higher OERs than the highest values in parental cultivars. These higher OERs showed positive correlation with the contents of Rubisco and/or Rubisco activase. Four lines of BC2F4 were generated by backcrossing wild rice with cultivar Nipponbare. The photosynthetic rates under saturated light and ambient air conditions in these 4 lines were average 7% higher than Nipponbare. Also, the grain yield per plant of BC2F4 lines were average 16% higher These results suggest that O.rufipogon can be used as a source of germplasm to improve the photosynthetic capacity and grain yield of O.sativa. In order to identify useful QTL alleles from wild rice relatives, we backcrossed with two typical rice cultivars, japonica Nipponbare and indica IR36. In the BC2 generation, several characters including photosynthetic activity were evaluated, and single-point QTL analysis was carried out using 74 microsatellite markers. From these analysis, two wild QTL alleles increasing photosynthetic activity was detected. The present results suggest that the wild QTL alleles are very useful in improving photosynthetic function and yield of rice.

  • 浮稲節間のジベレリンによる細胞伸長と細胞壁合成の関係
    東 哲司
    日本学術振興会, 科学研究費助成事業, 奨励研究(A), 神戸大学, 1999 - 2000
    UDP-糖を可逆的に結合する能力がありゴルジ体膜に存在することよりヘミセルロース性多糖の合成に関与している可能性が示唆されているReversibly Glycosylated Polypeptide(RGP)と細胞伸長との関係を明らかにするため,ジベレリン処理により節間の急激な伸長能力を示す浮稲茎切片を実験材料に用いて研究を行い,以下の結果が得られた. 1 アラビドプシスから得られたRGP抗体を用いて,イネ節間中の伸長に伴うRGPタンパク量の変化を調べた.イネ節間中の可溶性タンパク質および膜タンパク質中のRGPのレベルは,節間の急速な伸長をもたらす浸水およびジベレリン処理によりほとんど変動しなかった.現在イネRGPタンパク質の抗体を作成中であり,これを用いて再調査する予定である. 2 イネRGP遺伝子(OsRGP)をクローニングした結果,イネにおいて二つのRGP遺伝子(OsRGP1,OsRGP2)が得られ,エンドウRGP(PsRGP1)との比較でOsRGP1では86%,OsRGP2では43%の相同性(アミノ酸レベル)が認められた. 3 OsRGP1の遺伝子特異的配列をプローブとして,この遺伝子の様々な器官での発現量を調べた.この遺伝子は,成長中の節間・幼葉鞘・根端で発現しており,節間では細胞の分裂組織と伸長部位で高い発現量が認められた.浮稲茎切片をジベレリン処理すると,処理8時間以降にOsRGP1のmRNAの蓄積が節間基部において認められた.以上の結果ならびに昨年度得られた結果より,OsRGP1遺伝子の発現は,イネの様々な器官の生長と相関があり,ジベレリンによって伸長が促進される節間組織での増大する細胞壁合成と関係があることが考えられる.

  • response to high-salt stress in Halophyte cells
    YASUDA Takeshi, AZUMA Tetsushi, UCHIDA Naotsugu
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 1997 - 1998
    Halophyte plants are able to grow under high saline environments. For examining cellular response of Halophyte cells to salt stress, cultured cells from 3 Atriplex species ; 1 entiformis (AL), halimus (AH) and halimus (AH) were treated with and without 0.2M NaCl, and polypeptide patterns were analyzed by 2-D electrophoresis. Significant increase of polypeptide spot were detected in insoluble protein fractions of salt treated Atriplex cells on NEPHGE/SDS electrophoresis. A polypeptide named SP1 was with a molecular mass of 27 kDa with pI over 8.5. The polypeptide was glycoprotein with Con A binding activity. Eluted proteins from electrophoretic spot of LH were used for antibody preparation by mice and N-terminal amino acid sequencing. mRNA were isolated from salt-treated LH cells and cDNA library was prepared, and library screening were done using a probe, which amplified by PCR on primers constructed from N-terminal amino-acid sequences. We have determined the nucleotide sequence of a full length cDNA for SP1 and submitted on the DDBJ/EMBL/GenBank databases (AB024338). Reduced amino acid sequences reveals that SP1 is 224-AA, 24 kDa and pI=9.72. A search of the GenBank data base for SP1 amino acid sequence showed similarity to Germin and oxalate-oxidase.

  • Activation Mechanisms in Rice Photosynthesis
    UCHIDA Naotsugu, AZUMA Tetsushi, YASUDA Takeshi
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for General Scientific Research (C), Kobe University, 1994 - 1995
    We investigated the activation mechanisms of rice photosynthesis in relation to Rubisco activase. (1) We purified Rubisco activase from rice leaves, and showed the existence of this enzyme in rice leaves. (2) The amounts of Rubisco activase and Rubisco, and several photosynthetic characteristics in rice plants grown under different nitrogen concentrations and light intensities were investigated. The amounts of Rubisco activase increased linearly with increasing nitrogen supply regardless of the growing light conditions, whereas that of Rubisco increased slightly under the shade condition. The amount of Rubisco activase was almost linearly correlated to the maximum rate of photosynthetic oxygen evolution and the carboxylation efficiency, an indicator of in vivo Rubisco activity. (3) Photosynthetic activity (OER) and the amounts of leaf constituents were investigated in a rice leaf in relation to its aging. The changing pattern of Rubisco activase content was most remarkable among the leaf constituents examined. The OER depended linearly on the Rubisco content below the value of 3 g m^<-2>, but tended to saturate above this value. On the contrary, the amount of Rubisco activase and the OER were linearly correlated during the life span of the leaf. Therefore, the in vivo Rubisco activity can be restricted by Rubisco activase content.

  • 浮稲における光合成産物の動態と伸長の調節機構
    山口 禎, 東 哲司, 内田 直次, 安田 武司
    日本学術振興会, 科学研究費助成事業, 一般研究(C), 神戸大学, 1993 - 1993
    浮稲は主として節間を伸長させることで深水に対応している.この急激な節間伸長の開始と同時に,光合成産物のシンクとソースのバランスを個体の生長に有利になるように変化させていくことが必要になる.本研究では,深水下における同化産物の転流と分配のの変化を明らかにするため,特定の葉に供与した安定同位体炭素の部位別の経時的変化と数種の転流関連の酵素活性を調べた.また,浮稲の節間伸長の機構を探る目的で,明暗両条件下での深水による節間空隙内のガス組成の変化を調べた. 5時間の同化期間中にラベルされた炭素は供与した葉と最上位の伸長中の葉に最も多く分布するが,深水下の24時間後には伸長し始めた最上位の節間部に多く分配された.また,これらの部位のシュクロース/デンプン比が深水下で大きくなり,葉のシュクロースホスフェイトシンターゼ活性と伸長中の節間を含む茎部のシュクロースシンターゼ活性も深水により増加した.これらの結果から,深水下の浮稲において,シンクである伸長中の節間の同化産物の要求量が高まると同時にソースである葉身のシュクロース形成率が高くなり,同化産物の転流が増大するものと考えられた. 浮稲茎切片を用いて明暗両条件下で深水処理した結果,明条件下では節間伸長するが暗条件下では伸長しなかった.明条件下では酸素と二酸化炭素濃度はほとんど変化せず,エチレン濃度は顕著に上昇した.暗条件下では酸素濃度が低下し,二酸化炭素とエチレン濃度は上昇した.また,暗条件下で酸素を供給すると節間の伸長が認められた.以上の結果から,従来から考えられてきた節間空隙内のエチレン濃度の上昇に酸素濃度の低下は必要なく,むしろ旺盛な節間伸長には多量の酸素の供給が不可欠であることを明らかにした.

  • Micropropagation by Somatic Embryogenesis of Coffea Species and Cryopreservation
    YASUDA Takeshi, YAMAGUCHI Tadashi, AZUMA Tetsushi, UCHIDA Naotugu
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for General Scientific Research (C), Kobe University, 1992 - 1993
    The embryogenic callus and somatic embryo of Coffea arabica and C.canephora can be induced by cytokinin alone from the section of young leaf of mature trees. The cytokinin induced callus has a high embryogenic potetial and is very useful for clonal propagation. As cytokinin, 2-isopentenyl-adenine(2iP)is the best, and benzyladenin(BA), zeatin and kinetin also are effective for somatic embryogenesis from coffee leaf slices. Additional auxins act inhibitory effect on somatic embryogenesis. Protoplast were prepared from embryogenic calluses from C.arabica and canephora and cultured on Gelrite medium with cytokinin as a plant regulator. Somatic embryos were regenerated from protoplasts. These simplified method for preparation and culture of protoplasts is very useful for cell fusion and genetic engeering. Somatic embryos of Coffea were successfully cryopreserved after being exposed to a series of preparative steps ; precultured on media with increasing concentration of sucrose, encapsulated in alginate beads, dehydrated up to 30% and pluged into liquid nitrogen.

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