SEARCH
Search Details
OGINO ChiakiGraduate School of Engineering / Department of Chemical Science and EngineeringProfessor
Researcher basic information
■ Research news■ Research Areas
Research activity information
■ Award- Oct. 2021 神戸大学, 第13回学長表彰(財務貢献者)
- Oct. 2019 神戸大学, 第11回学長表彰(財務貢献者)
- Oct. 2018 神戸大学, 第10回学長表彰(財務貢献者)
- Oct. 2017 神戸大学, 第9回学長表彰(財務貢献者)
- Sep. 2013 日本生物工学会, 生物工学論文賞, Direct isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display systemJapan society
- Mar. 2007 化学工学会, 平成18年度化学工学会 奨励賞(實吉雅郎記念賞), リン脂質修飾酵素ホスホリパーゼDに関する基礎解析および生産技術の開発
- Aug. 2024, Biochemical Engineering Journal[Refereed]Scientific journal
- Jun. 2024, Engineering in Life Sciences[Refereed]Scientific journal
- Previously, we synthesized an evolved version of a bio-based polylactide (PLA) on microbial platforms using our engineered lactate-polymerizing enzyme (LPE). This lactate (LA)-based copolyester, LAHB, has advantages over PLA, including improved flexibility and biodegradability, and its properties can be regulated through the LA fraction. To expand the LA-incorporation capacity and improve polymer properties, in the state of in vivo LAHB production, propionyl-CoA transferases (PCTs) that exhibited enhanced production of LA-CoA than the conventional PCTs were selected. Here, the present study has demonstrated that the LA fraction of LAHB could be altered using various PCTs. Enhanced PCT performance was achieved by balancing polymer production and cell growth. Both events are governed by the use of acetyl-CoA, a commonly shared key metabolite. This could be attributed to the different reactivities of individual PCTs towards acetyl-CoA, which serves both as a CoA donor and a leading compound in the TCA cycle. Interestingly, we found complete sequence randomness in the LAHB copolymers, independent of the LA fraction. The mechanism of LA fraction-independent sequence randomness is discussed. This new PCT-based strategy synergistically combines with the evolution of LPE to advance the LAHB project, and enables us to perform advanced applications other than LAHB production utilizing CoA-linked substrates.Jun. 2024, International journal of biological macromolecules, 133055 - 133055, English, International magazineScientific journal
- Only a few reports available about the assimilation of hydrophobic or oil-based feedstock as carbon sources by Lipomyces starkeyi. In this study, the ability of L. starkeyi to efficiently utilize free fatty acids (FFAs) and real biomass like palm acid oil (PAO) as well as crude palm kernel oil (CPKO) for growth and lipid production was investigated. PAO, CPKO, and FFAs were evaluated as sole carbon sources or in the mixed medium containing glucose. L. starkeyi was able to grow on the medium supplemented with PAO and FFAs, which contained long-chain length FAs and accumulated lipids up to 35% (w/w) of its dry cell weight. The highest lipid content and lipid concentration were achieved at 50% (w/w) and 10.1 g/L, respectively, when L. starkeyi was cultured in nitrogen-limited mineral medium (-NMM) supplemented with PAO emulsion. Hydrophobic substrate like PAO could be served as promising carbon source for L. starkeyi.May 2024, Journal of bioscience and bioengineering, English, Domestic magazineScientific journal
- While flocculation has demonstrated its efficacy in enhancing yeast robustness and ethanol production, its potential application for lactic acid fermentation remains largely unexplored. Our study examined the differences between flocculating and nonflocculating Saccharomyces cerevisiae strains in terms of their metabolic dynamics when incorporating an exogenous lactic acid pathway, across varying cell densities and in the presence of lignocellulose-derived byproducts. Comparative gene expression profiles revealed that cultivating a nonflocculant strain at higher cell density yielded a substantial upregulation of genes associated with glycolysis, energy metabolism, and other key pathways, resulting in elevated levels of fermentation products. Meanwhile, the flocculating strain displayed an inherent ability to sustain high glycolytic activity regardless of the cell density. Moreover, our investigation revealed a significant reduction in glycolytic activity under chemical stress, potentially attributable to diminished ATP supply during the energy investment phase. Conversely, the formation of flocs in the flocculating strain conferred protection against toxic chemicals present in the medium, fostering more stable lactic acid production levels. Additionally, the distinct flocculation traits observed between the two examined strains may be attributed to variations in the nucleotide sequences of the flocculin genes and their regulators. This study uncovers the potential of flocculation for enhanced lactic acid production in yeast, offering insights into metabolic mechanisms and potential gene targets for strain improvement.Apr. 2024, Yeast (Chichester, England), 41(4) (4), 192 - 206, English, International magazineScientific journal
- Itaconic acid is a promising biochemical building block that can be used in polymer synthesis. Itaconic acid is currently produced in industry by the natural producer fungus Aspergillus terreus using glucose as a main carbon source. Most research for itaconic acid production using lignocellulosic-based carbon sources was carried out by A. terreus. Engineered Corynebacterium glutamicum strain which can grow in presence of fermentation inhibitors without effect on growth, was used for production of itaconic acid using sweet sorghum juice and bagasse sugar lysate (BSL). BSL contains many inhibitors unlike sorghum juice. C. glutamicum could grow in the media containing both types of lignocellulose-based carbon sources without showing any growth inhibition, however, sorghum juice was better in itaconic acid production than BSL. Different constructed strains of C. glutamicum were used for itaconic acid production, however, C. glutamicum ATCC 13032 pCH-Tad1optAdi1opt strain expressing Adi1/Tad1 genes (trans-pathway) from Ustilago maydis proved to be better in itaconic acid production giving final titer of 8.4 and 4.02 g/L using sweet sorghum juice and BSL as the sole carbon sources by fed-batch fermentation. Our study is the first for production of itaconic acid using sweet sorghum juice and BSL. The present study also proved that C. glutamicum can be used for enhancing itaconic acid production using lignocellulosic-based carbon sources.Jan. 2024, Enzyme and microbial technology, 172, 110345 - 110345, English, International magazineScientific journal
- Dec. 2023, AIP Conference Proceedings, 2972(1) (1)International conference proceedings
- Abstract Cellulose has been developed as an alternative to petrochemical materials. By comparison with refined nanofibers (RCNFs), lignocellulose nanofibers (LCNFs) show particular promise because it is produced from biomass using only mild pretreatment. The mechanical properties of LCNFs depend on the contained lignin. However, the microscopic location of the lignin contained in LCNFs has not been determined. Thus, we developed two methods to detect and visualize lignin. One uses a scanning transmission electron microscope (STEM) equipped with an energy dispersive X-ray spectroscopy detector. The other method uses an atomic force microscope (AFM) equipped with a cantilever coated with an aromatic molecule. Both methods revealed that the lignin in LCNFs covers a thin cellulose fiber and is precipitated in a grained structure. In particular, the AFM system was able to determine the nanoscopic location of lignin-rich areas. The present study establishes a strong tool for analyzing the characteristics of lignin-containing materials. Graphical abstractSpringer Science and Business Media LLC, Nov. 2023, Cellulose, 30(18) (18), 11357 - 11367Scientific journal
- Nov. 2023, Biochemical Engineering Journal[Refereed]Scientific journal
- Itaconic acid (IA) is a value-added chemical currently produced by Aspergillus terreus from edible glucose and starch but not from inedible lignocellulosic biomass owing to the high sensitivity to fermentation inhibitors present in the hydrolysate of lignocellulosic biomass. To produce IA from lignocellulosic biomass, a gram-positive bacterium, Corynebacterium glutamicum, with a high tolerance to fermentation inhibitors was metabolically engineered to express a fusion protein composed of cis-aconitate decarboxylase from A. terreus responsible for IA formation from cis-aconitate and a maltose-binding protein (malE) from Escherichia coli. The codon-optimized cadA_malE gene was expressed in C. glutamicum ATCC 13032, and the resulting recombinant strain produced IA from glucose. IA concentration increased 4.7-fold by the deletion of the ldh gene encoding lactate dehydrogenase. With the Δldh strain HKC2029, an 18-fold higher IA production was observed from enzymatic hydrolysate of kraft pulp as a model lignocellulosic biomass than from glucose (6.15 and 0.34 g/L, respectively). The enzymatic hydrolysate of kraft pulp contained various potential fermentation inhibitors involved in furan aldehydes, benzaldehydes, benzoic acids, cinnamic acid derivatives, and aliphatic acid. Whereas cinnamic acid derivatives severely inhibited IA production, furan aldehydes, benzoic acids, and aliphatic acid improved IA production at low concentrations. The present study suggests that lignocellulosic hydrolysate contains various potential fermentation inhibitors; however, some of them can serve as enhancers for microbial fermentation likely due to the changing of redox balance in the cell.Jul. 2023, Journal of bioscience and bioengineering, 136(1) (1), 7 - 12, English, Domestic magazineScientific journal
- Itaconic acid (IA), a C5-dicarboxylic acid, is a potential bio-based building block for the polymer industry. There are three pathways for IA production from natural IA producers; however, most of the engineered strains were used for IA production by heterologous expression of cis-aconitate decarboxylase gene (cadA) from Aspergillus terreus. In this study, IA was produced by an engineered Corynebacterium glutamicum ATCC 13032 expressing two different types of genes from two distinct pathways. The first involves the mammalian immunoresponsive gene1 (Irg1) derived from Mus musculus. The second (termed here the trans-pathway) involves two genes from the natural IA producer Ustilago maydis which are aconitate-delta-isomerase (Adi1) and trans-aconitate decarboxylase (Tad1) genes. The constructed strains developing the two distinct IA production pathways: C. glutamicum ATCC 13032 pCH-Irg1opt and C. glutamicum ATCC 13032 pCH-Tad1optadi1opt were used for production of IA from different carbon sources. The results reflect the possibility for IA production from C. glutamicum expressing the trans-pathway (Adi1/Tad1 genes) and cis-pathway (Irg1 gene) other than the well-known cis-pathway that depends mainly on cadA gene from A. terreus. The developed strain expressing trans-pathway from U. maydis; however, proved to be better at IA production with high titers of 12.25, 11.34, and 11.02 g/L, and a molar yield of 0.22, 0.42, and 0.43 mol/mol from glucose, maltose, and sucrose, respectively, via fed-batch fermentation. The present study suggests that trans-pathway is better than cis-pathway for IA production in engineered C. glutamicum.Jun. 2023, Journal of bioscience and bioengineering, 136(2) (2), 109 - 116, English, Domestic magazineScientific journal
- Plants produce sugars by photosynthesis and use them for growth and development. Sugars are transported from source-to-sink organs via the phloem in the vasculature. It is well known that vascular development is precisely controlled by plant hormones and peptide hormones. However, the role of sugars in the regulation of vascular development is poorly understood. In this study, we examined the effects of sugars on vascular cell differentiation using a vascular cell induction system named Vascular cell Induction culture System Using Arabidopsis Leaves (VISUAL). We found that sucrose has the strongest inhibitory effect on xylem differentiation among several types of sugars. Transcriptome analysis revealed that sucrose suppresses xylem and phloem differentiation from cambial cells. Physiological and genetic analysis suggested that sucrose might function through the BES1 transcription factor, which is the central regulator of vascular cell differentiation. Conditional overexpression of cytosolic invertase led to a decrease in the number of cambium layers due to an imbalance between cell division and differentiation. Taken together, our results suggest that sucrose potentially acts as a signal that integrates environmental conditions with the developmental program.May 2023, Plant & cell physiology, 64(12) (12), 1511 - 1522, English, Domestic magazineScientific journal
- Sorghum is the fifth most important cereal crop. Here we performed molecular genetic analyses of the ‘SUGARY FETERITA’ (SUF) variety, which shows typical sugary endosperm traits (e.g., wrinkled seeds, accumulation of soluble sugars, and distorted starch). Positional mapping indicated that the corresponding gene was located on the long arm of chromosome 7. Within the candidate region of 3.4 Mb, a sorghum ortholog for maize Su1 (SbSu) encoding a starch debranching enzyme ISA1 was found. Sequencing analysis of SbSu in SUF uncovered nonsynonymous single nucleotide polymorphisms (SNPs) in the coding region, containing substitutions of highly conserved amino acids. Complementation of the rice sugary-1 (osisa1) mutant line with the SbSu gene recovered the sugary endosperm phenotype. Additionally, analyzing mutants obtained from an EMS-induced mutant panel revealed novel alleles with phenotypes showing less severe wrinkles and higher Brix scores. These results suggested that SbSu was the corresponding gene for the sugary endosperm. Expression profiles of starch synthesis genes during the grain-filling stage demonstrated that a loss-of-function of SbSu affects the expression of most starch synthesis genes and revealed the fine-tuned gene regulation in the starch synthetic pathway in sorghum. Haplotype analysis using 187 diverse accessions from a sorghum panel revealed the haplotype of SUF showing severe phenotype had not been used among the landraces and modern varieties. Thus, weak alleles (showing sweet and less severe wrinkles), such as in the abovementioned EMS-induced mutants, are more valuable for grain sorghum breeding. Our study suggests that more moderate alleles (e.g. produced by genome editing) should be beneficial for improving grain sorghum.Frontiers Media SA, Feb. 2023, Frontiers in Plant Science, 14, 1114935 - 1114935, English, International magazineScientific journal
- Lignocellulose is resistant to degradation and requires pretreatment before hydrolytic enzymes can release fermentable sugars. Sulfuric acid has been widely used for biomass pretreatment, but high amount of degradation products usually occurred when using this method. To enhance accessibility to cellulose, we studied the performances of several dilute organic acid pretreatments of sugarcane bagasse and oil palm empty fruit bunch fiber. The results revealed that pretreatment with maleic acid yields the highest xylose and glucose release among other organic acids. The effects of concentration, duration of heating and heating temperature were further studied. Dilute maleic acid 1% (w/w) pretreatment at 180 °C was the key to its viability as a substitute for sulfuric acid. Moreover, maleic acid did not seem to highly promote the formation of either furfural or 5-HMF in the liquid hydrolysate after pretreatment.Elsevier BV, Feb. 2023, Bioresource Technology, 369, 128382 - 128382, English, International magazine[Refereed]Scientific journal
- Wiley, Jan. 2023, Small, 19(14) (14), 2207318 - 2207318, English, International magazine[Refereed]Scientific journal
- 4-Aminophenylalanine as a diamine monomer to synthesize unique biopolyimides was produced from lignocellulosic biomass by microbial fermentation. Fermentation inhibitors present in lignocellulosic hydrolysate of sorghum bagasse were identified.Royal Society of Chemistry (RSC), 2023, RSC SustainabilityScientific journal
- Abstract The bottleneck for the production of biofuels from microalgae consists on costly harvesting processes and low lipid production, immobilization technology could play a part on making the production of biofuels more feasible. The aim of this study was to evaluate the effect of alginate immobilization on the growth and lipid productivity of the microalgae Chlorella sorokiniana, so far, the main focus of immobilization technology has been its use for wastewater treatment and nutrient removal from effluents. The microalgae Chlorella sorokiniana was cultured in both free and immobilized forms under optimal autotrophic growth conditions. Microalgae were immobilized in calcium alginate beads generated by mixing algal cells with a sodium alginate solution, followed by extrusion into a CaCl2 solution. The results obtained in this study showed that the growth of the microalgae immobilized in alginate beads, was enhanced and achieved a dry cell weight 1.4-fold higher than that of a free cell culture, a higher light transmittance was also achieved in the alginate immobilized culture, and the lipid productivity was increased from 54.21 ± 2.48 mg l−1 d in the free cell culture to 82.22 ± 8.48 mg l−1 d in the immobilized culture. These results demonstrate the effectiveness of immobilization technology for promoting growth and lipid productivity in the microalgae Chlorella sorokiniana.IOP Publishing, Jan. 2023, Journal of Physics: Energy, 5(1) (1), 014019 - 014019, English[Refereed]Scientific journal
- Elsevier BV, Dec. 2022, Biochemical Engineering Journal, 188, 108681 - 108681, English[Refereed]Scientific journal
- Sep. 2022, Journal of Physical Chemistry B, 126(36) (36), 6762 - 6770, English, International magazineScientific journal
- New enzyme functions exist within the increasing number of unannotated protein sequences. Novel enzyme discovery is necessary to expand the pathways that can be accessed by metabolic engineering for the biosynthesis of functional compounds. Accordingly, various machine learning models have been developed to predict enzymatic reactions. However, the ability to predict unknown reactions that are not included in the training data has not been clarified. In order to cover uncertain and unknown reactions, a wider range of reaction types must be demonstrated by the models. Here, we establish 16 expanded enzymatic reaction prediction models developed using various machine learning algorithms, including deep neural network. Improvements in prediction performances over that of our previous study indicate that the updated methods are more effective for the prediction of enzymatic reactions. Overall, the deep neural network model trained with combined substrate-enzyme-product information exhibits the highest prediction accuracy with Macro F1 scores up to 0.966 and with robust prediction of unknown enzymatic reactions that are not included in the training data. This model can predict more extensive enzymatic reactions in comparison to previously reported models. This study will facilitate the discovery of new enzymes for the production of useful substances.Sep. 2022, The journal of physical chemistry. B, 126(36) (36), 6762 - 6770, English, International magazineScientific journal
- Acidic and chemical inhibitor stresses undermine efficient lactic acid bioproduction from lignocellulosic feedstock. Requisite coping treatments, such as detoxification and neutralizing agent supplementation, can be eliminated if a strong microbial host is employed in the process. Here, we exploited an originally robust yeast, Saccharomyces cerevisiae BTCC3, as a production platform for lactic acid. This wild-type strain exhibited a rapid cell growth in the presence of various chemical inhibitors compared to laboratory and industrial strains, namely BY4741 and Ethanol-red. Pathway engineering was performed on the strain by introducing an exogenous LDH gene after disrupting the PDC1 and PDC5 genes. Facilitated by this engineered strain, high cell density cultivation could generate lactic acid with productivity at 4.80 and 3.68 g L-1 h-1 under semi-neutralized and non-neutralized conditions, respectively. Those values were relatively higher compared to other studies. Cultivation using real lignocellulosic hydrolysate was conducted to assess the performance of this engineered strain. Non-neutralized fermentation using non-detoxified hydrolysate from sugarcane bagasse as a medium could produce lactic acid at 1.69 g L-1 h-1, which was competitive to the results from other reports that still included detoxification and neutralization steps in their experiments. This strategy could make the overall lactic acid bioproduction process simpler, greener, and more cost-efficient.Springer Science and Business Media LLC, Aug. 2022, Scientific reports, 12(1) (1), 13645 - 13645, English, International magazineScientific journal
- When lignocellulosic biomass is utilized as a fermentative substrate to produce biochemicals, the existence of a yeast strain resistant to inhibitory chemical compounds (ICCs) released from the biomass becomes critical. To achieve the purpose, in this study, Saccharomyces yeast strains from a NBRC yeast culture collection were used for exploration and evaluated in two different media containing ICCs that mimic one another but resemble the hydrolysate of real biomass. Among them, S. cerevisiae F118 strain shows robustness upon the fermentation with unique flocculation trait that was strongly responsive to ICC stress. When this strain was cultured in the presence of ICCs, its cell wall hydrophobicity increased dramatically, and reduced significantly when the ICCs were depleted, demonstrating that cell-surface hydrophobicity can also act as an adaptive response to the ICCs. Cells from the strain with the highest cell-wall hydrophobicity displayed progressively stronger flocculation, indicating that the F118 strain is having unique robustness under ICC stress. Gene expression perturbation analysis revealed that mot3 gene encoding regulatory Mot3p from the F118 strain was expressed in response to the concentration of ICCs. This gene was found to control expression of ygp1 gene that encoding Ygp1p, one of cell wall proteins. Deep sequencing analysis revealed that the Mot3p of the F118 strain features a unique insertion and deletion of nucleotides that encode glutamine or asparagine residues, particularly in N-terminal domain, as determined by comparison to the Mot3p sequence from the S288c strain, which was employed as a control strain. Furthermore, the cell wall hydrophobicity of the S288c strain was greatly enhanced and became ICC-responsive after gene swapping with the mot3 gene from the F118 strain. The gene-swapped S288c strain fermented 6-fold faster than the wild-type strain, producing 14.5 g/L of ethanol from 30 g/L of glucose consumed within 24 h in a medium containing the ICCs. These such modifications to Mot3p in unique locations in its sequence have a potential to change the expression of a gene involved in cell wall hydrophobicity and boosted the flocculation response to ICC stress, allowing for the acquisition of extraordinary robustness.Elsevier BV, Jul. 2022, Metabolic Engineering, 72, 82 - 96, English, International magazineScientific journal
- Elsevier BV, Jun. 2022, Journal of Environmental Chemical Engineering, 10(3) (3), 107550 - 107550, English[Refereed]Scientific journal
- Apr. 2022, JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 41(1) (1), 146 - 146, English, International magazineScientific journal
- Feb. 2022, PROCESSES, 10(2) (2), EnglishScientific journal
- The aromatic compound 3-amino-4-hydroxybenzoic acid (3,4-AHBA) can be employed as a raw material for high-performance industrial plastics. The aim of this study is to produce 3,4-AHBA via a recombinant Streptomyces lividans strain containing griI and griH genes derived from Streptomyces griseus using culture medium with glucose and/or xylose, which are the main components in lignocellulosic biomass. Production of 3,4-AHBA by the recombinant S. lividans strain was successful, and the productivity was affected by the kind of sugar used as an additional carbon source. Metabolic profiles revealed that L aspartate-4-semialdehyde (ASA), a precursor of 3,4-AHBA, and coenzyme NADPH were supplied in greater amounts in xylose medium than in glucose medium. Moreover, cultivation in TSB medium with a mixed sugar (glucose/xylose) was found to be effective for 3,4-AHBA production, and optimal conditions for efficient production were designed by changing the ratio of glucose to xylose. The best productivity of 2.70 g/L was achieved using a sugar mixture of 25 g/L glucose and 25 g/L xylose, which was 1.5 times higher than the result using 50 g/L glucose alone. These results suggest that Streptomyces is a suitable candidate platform for 3,4-AHBA production from lignocellulosic biomass-derived sugars under appropriate culture conditions.Microbiology Research Foundation, 2022, The Journal of General and Applied Microbiology, 68(2) (2), 109 - 116, English, Domestic magazine[Refereed]Scientific journal
- Each year, the palm oil industry generates a significant amount of biomass residue and effluent waste; both have been identified as significant sources of greenhouse gas (GHG) emissions. This issue poses a severe environmental challenge for the industry due to the possibility of long-term negative effects on human well-being. The palm-oil industry must invest significantly in the technology that is required to resolve these issues and to increase the industry's sustainability. However, current technologies for converting wastes such as lignocellulosic components and effluents into biochemical products are insufficient for optimal utilization. This review discusses the geographical availability of palm-oil biomass, its current utilization routes, and then recommends the development of technology for converting palm-oil biomass into value-added products through an integrated biorefinery strategy. Additionally, this review summarizes the palm oil industry's contribution to achieving sustainable development goals (SDGs) through a circular bioeconomy concept.Elsevier BV, Jan. 2022, Bioresource Technology, 344(Pt B) (Pt B), 126266 - 126266, English, International magazineScientific journal
- Lignocellulosic biomass has great potential as an inedible feedstock for bioplastic synthesis, although its use is still limited compared to current edible feedstocks of glucose and starch. This review focuses on recent advances in the production of biopolymers and biomonomers from lignocellulosic feedstocks with downstream processing and chemical polymer syntheses. In microbial production, four routes composed of existing poly (lactic acid) and polyhydroxyalkanoates (PHAs) and the emerging biomonomers of itaconic acid and aromatic compounds were presented to review present challenges and future perspectives, focusing on the use of lignocellulosic feedstocks. Recently, advances in purification technologies decreased the number of processes and their environmental burden. Additionally, the unique structures and high-performance of emerging lignocellulose-based bioplastics have expanded the possibilities for the use of bioplastics. The sequence of processes provides insight into the emerging technologies that are needed for the practical use of bioplastics made from lignocellulosic biomass.Elsevier BV, Jan. 2022, Bioresource Technology, 344(Pt B) (Pt B), 126165 - 126165, English, International magazineScientific journal
- Springer Science and Business Media LLC, Dec. 2021, Microbial Cell Factories, 20(1) (1), 228 - 228, English, International magazine
Abstract Background Bio-based aromatic compounds are of great interest to the industry, as commercial production of aromatic compounds depends exclusively on the unsustainable use of fossil resources or extraction from plant resources. γ-amino acid 3-amino-4-hydroxybenzoic acid (3,4-AHBA) serves as a precursor for thermostable bioplastics.Results Under aerobic conditions, a recombinantCorynebacterium glutamicum strain KT01 expressinggriH andgriI genes derived fromStreptomyces griseus produced 3,4-AHBA with large amounts of amino acids as by-products. The specific productivity of 3,4-AHBA increased with decreasing levels of dissolved oxygen (DO) and was eightfold higher under oxygen limitation (DO = 0 ppm) than under aerobic conditions (DO ≥ 2.6 ppm). Metabolic profiles during 3,4-AHBA production were compared at three different DO levels (0, 2.6, and 5.3 ppm) using the DO-stat method. Results of the metabolome analysis revealed metabolic shifts in both the central metabolic pathway and amino acid metabolism at a DO of < 33% saturated oxygen. Based on this metabolome analysis, metabolic pathways were rationally designed for oxygen limitation. Anldh deletion mutant, with the loss of lactate dehydrogenase, exhibited 3.7-fold higher specific productivity of 3,4-AHBA at DO = 0 ppm as compared to the parent strain KT01 and produced 5.6 g/L 3,4-AHBA in a glucose fed-batch culture.Conclusions Our results revealed changes in the metabolic state in response to DO concentration and provided insights into oxygen supply during fermentation and the rational design of metabolic pathways for improved production of related amino acids and their derivatives.Graphical Abstract Scientific journal - Recently, a hyphae-dispersed type of filamentous fungus Aspergillus oryzae was constructed via genetic engineering, and industrial applications are expected due to the ease of handling and to the level of protein production properties. In this study, we constructed cellulase-expressing strains using wild-type and hyphae-dispersed strains to investigate the correlation between protein productivity and metabolism. Compared with the original strain, the hyphae-dispersed cellulase-expressing strain showed elevated cellulase activity, rapid glucose consumption, increased mycelial dry weight, an increased expression of cellulase genes, and activated respiration activity. Comparative metabolomic analysis showed fewer metabolites in the glycolysis and TCA cycles in the dispersed strains than in the original strains. These results indicate that the flux of carbohydrate metabolism in the hyphae-dispersed strains is smoother than that in the original strains. Such efficient metabolic flux would contribute to efficient energy conversion and to sufficient energy supply to anabolisms, such as mycelial growth and protein production. Our findings suggest that the hyphae-dispersed strains could be a useful host not only for protein production but also for the biological production of various chemicals such as organic acids.Elsevier BV, Aug. 2021, Journal of Bioscience and Bioengineering, 132(2) (2), 140 - 147, English, Domestic magazineScientific journal
- Wiley, May 2021, Biofuels, Bioproducts and Biorefining, 15(3) (3), 804 - 814, EnglishScientific journal
- (一社)日本インターベンショナルラジオロジー学会, Apr. 2021, 日本インターベンショナルラジオロジー学会雑誌, 36(Suppl.) (Suppl.), 191 - 191, JapaneseウサギVX2腫瘍モデルを用いた過酸化チタンナノ粒子動注の基礎的研究
- Polyacrylic acid-modified titanium peroxide nanoparticles (PAA-TiOx NPs) are promising radiosensitizers that enhance the therapeutic effect of X-ray irradiation after local injection into tumors. However, the mechanism for this reaction has remained unclear with the exception of the involvement of hydrogen peroxide (H2O2), which is released by PAA-TiOx NPs to a liquid phase during dispersion. In the present study, a clonogenic assay was used to compare PAA-TiOx NPs with free H2O2 molecules to investigate the effect exerted on the radiosensitivity of cancer cells in vitro. A cell-free dialysis method revealed that a portion of the H2O2 adsorbed onto the PAA-TiOx NPs during synthesis could be released during a treatment regimen. The H2O2 release lasted for 7 h, which was sufficient for one radiation treatment procedure. For in vitro experiments, cultured human pancreatic cancer cells took up PAA-TiOx NPs in 10 min after administration. Interestingly, when the cells were washed with a buffer after treatment with either a PAA-TiOx NP or H2O2 solution, the intracellular H2O2 levels remained higher with PAA-TiOx NP treatment compared with the H2O2 solution treatment. Furthermore, the effects of subsequent X-ray irradiation corresponded to the intracellular H2O2 levels. These results indicate that PAA-TiOx NPs are efficient carriers of H2O2 into cancer cells and thus enhance the radiosensitivity.Elsevier BV, Feb. 2021, Colloids and Surfaces B: Biointerfaces, 198, 111451 - 111451, English, International magazineScientific journal
- We describe a novel expression cassette that enables efficient and constitutive expression of the ZZ domain derived from Staphylococcus aureus protein A on the yeast cell surface to easily prepare yeast-based immunosorbents. Using this expression cassette containing the PGK1 promoter, a secretion signal derived from α-factor, and a Flo1-derived anchor protein, we successfully created a yeast-based immunosorbent for human serum albumin.Microbiology Research Foundation, 2021, The Journal of General and Applied Microbiology, 67(6) (6), 265 - 268, English, Domestic magazineScientific journal
- Wiley, Jan. 2021, Advanced Sustainable Systems, 5(1) (1), 2000193 - 2000193Scientific journal
- Cambridge University Press ({CUP}), Dec. 2020, MRS Communications, 10(4) (4), 680 - 686[Refereed]Scientific journal
- Elsevier BV, Dec. 2020, Renewable and Sustainable Energy Reviews, 134, 110355 - 110355Scientific journal
- American Chemical Society (ACS), Nov. 2020, ACS Applied Bio Materials, 3(11) (11), 7743 - 7751Scientific journal
- Nov. 2020, Biomass and Bioenergy, 142, 105768 - 105768, English[Refereed]Scientific journal
- Pyruvate is a central metabolite for the biological production of various chemicals. In eukaryotes, pyruvate produced by glycolysis is used in conversion to ethanol and lactate and in anabolic metabolism in the cytosol, or is transported into the mitochondria for use as a substrate in the tricarboxylic acid (TCA) cycle. In this study, we focused on controlling pyruvate metabolism in aerobic microorganisms for the biological production of various chemicals. We successfully improved productivity by redirecting pyruvate metabolism in the aerobic filamentous fungus Aspergillus oryzae via the deletion of two genes that encode pyruvate decarboxylase and mitochondrial pyruvate carriers. Production of ethanol as a major byproduct was completely inhibited, and the limited translocation of pyruvate into the mitochondria shifted the metabolism from respiration for energy conversion to the effective production of lactate or 2,3-butandiole, even under aerobic conditions. Metabolomic and transcriptomic analyses showed an emphasis on glycolysis and a repressed TCA cycle. Although the dry mycelial weights of the deletion mutants were reduced compared with those of wild type, the titer and yields of the target products were drastically increased. In particular, the redirection of pyruvate metabolism shifted from anabolism for biomass production to catabolism for the production of target chemicals. Conclusively, our results indicate that the redirection of pyruvate metabolism is a useful strategy in the metabolic engineering of aerobic microorganisms.Jul. 2020, Metabolic engineering, English, International magazine[Refereed]Scientific journal
- The development of potentially safe radiosensitizing agents is essential to enhance the treatment outcomes of radioresistant cancers. The titanium peroxide nanoparticle (TiOxNP) was originally produced using the titanium dioxide nanoparticle, and it showed excellent reactive oxygen species (ROS) generation in response to ionizing radiation. Surface coating the TiOxNPs with polyacrylic acid (PAA) showed low toxicity to the living body and excellent radiosensitizing effect on cancer cells. Herein, we evaluated the mechanism of radiosensitization by PAA-TiOxNPs in comparison with gold nanoparticles (AuNPs) which represent high-atomic-number nanoparticles that show a radiosensitizing effect through the emission of secondary electrons. The anticancer effects of both nanoparticles were compared by induction of apoptosis, colony-forming assay, and the inhibition of tumor growth. PAA-TiOxNPs showed a significantly more radiosensitizing effect than that of AuNPs. A comparison of the types and amounts of ROS generated showed that hydrogen peroxide generation by PAA-TiOxNPs was the major factor that contributed to the nanoparticle radiosensitization. Importantly, PAA-TiOxNPs were generally nontoxic to healthy mice and caused no histological abnormalities in the liver, kidney, lung, and heart tissues.Jun. 2020, Nanomaterials (Basel, Switzerland), 10(6) (6), English, International magazineScientific journal
- May 2020, Processes, 8(5) (5), 619, EnglishHigh enzymatic recovery and purification of xylooligosaccharides from empty fruit bunch via nanofiltration[Refereed]Scientific journal
- Apr. 2020, Processes, 8(4) (4), 450, EnglishConcentration of Lipase from Aspergillus oryzae Expressing Fusarium heterosporum by Nanofiltration to Enhance Transesterification[Refereed]
- Unannotated gene sequences in databases are increasing due to sequencing advances. Therefore, computational methods to predict functions of unannotated genes are needed. Moreover, novel enzyme discovery for metabolic engineering applications further encourages annotation of sequences. Here, enzyme functions are predicted using two general approaches, each including several machine learning algorithms. First, Enzyme-models (E-models) predict Enzyme Commission (EC) numbers from amino acid sequence information. Second, Substrate-Enzyme models (SE-models) are built to predict substrates of enzymatic reactions together with EC numbers, and Substrate-Enzyme-Product models (SEP-models) are built to predict substrates, products, and EC numbers. While accuracy of E-models is not optimal, SE-models and SEP-models predict EC numbers and reactions with high accuracy using all tested machine learning-based methods. For example, a single Random Forests-based SEP-model predicts EC first digits with an Average AUC score of over 0.94. Various metrics indicate that the current strategy of combining sequence and chemical structure information is effective at improving enzyme reaction prediction.Mar. 2020, Journal of chemical information and modeling, 60(3) (3), 1833 - 1843, English, International magazine[Refereed]Scientific journal
- Mar. 2020, SUSTAINABLE ENERGY & FUELS, 4(3) (3), 1105 - 1111, English[Refereed]Scientific journal
- Mar. 2020, Fuel Processing Technology, 199, 106278, EnglishBiodiesel-mediated biodiesel production: A recombinant Fusarium heterosporum lipase-catalyzed transesterification of crude plant oils[Refereed]Scientific journal
- Microalgae biomass contains various useful bio-active components. Microalgae derived biodiesel has been researched for almost two decades. However, sole biodiesel extraction from microalgae is time-consuming and is not economically feasible due to competitive fossil fuel prices. Microalgae also contains proteins and carbohydrates in abundance. Microalgae are likewise utilized to extract high-value products such as pigments, anti-oxidants and long-chain polyunsaturated fatty acids which are useful in cosmetic, pharmaceutical and nutraceutical industry. These compounds can be extracted simultaneously or sequentially after biodiesel extraction to reduce the total expenditure involved in the process. This approach of bio-refinery is necessary to promote microalgae in the commercial market. Researchers have been keen on utilizing the bio-refinery approach to exploit the valuable components encased by microalgae. Apart from all the beneficial components housed by microalgae, they also help in reducing the anthropogenic CO2 levels of the atmosphere while utilizing saline or wastewater. These benefits enable microalgae as a potential source for bio-refinery approach. Although life-cycle analysis and economic assessment do not favor the use of microalgae biomass feedstock to produce biofuel and co-products with the existing techniques, this review still aims to highlight the beneficial components of microalgae and their importance to humans. In addition, this article also focuses on current and future aspects of improving the feasibility of bio-processing for microalgae bio-refinery.Dec. 2019, Bioengineered, 10(1) (1), 574 - 592, English, International magazine[Refereed]Scientific journal
- Sep. 2019, BIOCHEMICAL ENGINEERING JOURNAL, 149[Refereed]
- Sep. 2019, BIOMASS & BIOENERGY, 128[Refereed]
- Xylitol is a highly valuable commodity chemical used extensively in the food and pharmaceutical industries. The production of xylitol from d-xylose involves a costly and polluting catalytic hydrogenation process. Biotechnological production from lignocellulosic biomass by micro-organisms like yeasts is a promising option. In this study, xylitol is produced from lignocellulosic biomass by a recombinant strain of Saccharomyces cerevisiae (S. cerevisiae) (YPH499-SsXR-AaBGL) expressing cytosolic xylose reductase (Scheffersomyces stipitis xylose reductase [SsXR]), along with a β-d-glucosidase (Aspergillus aculeatus β-glucosidase 1 [AaBGL]) displayed on the cell surface. The simultaneous cofermentation of cellobiose/xylose by this strain leads to an ≈2.5-fold increase in Yxylitol/xylose (=0.54) compared to the use of a glucose/xylose mixture as a substrate. Further improvement in the xylose uptake by the cell is achieved by a broad evaluation of several homologous and heterologous transporters. Homologous maltose transporter (ScMAL11) shows the best performance in xylose transport and is used to generate the strain YPH499-XR-ScMAL11-BGL with a significantly improved xylitol production capacity from cellobiose/xylose coutilization. This report constitutes a promising proof of concept to further scale up the biorefinery industrial production of xylitol from lignocellulose by combining cell surface and metabolic engineering in S. cerevisiae.Sep. 2019, Biotechnology journal, 14(9) (9), e1800704, English, International magazine[Refereed]Scientific journal
- Jul. 2019, Nature communications, 10(1) (1), 3132 - 3132, English, International magazine[Refereed]
- Jul. 2019, ACS Sustainable Chemistry and Engineering, 7(13) (13), 11069 - 11079[Refereed]Scientific journal
- Jun. 2019, MATERIALS TODAY COMMUNICATIONS, 19, 39 - 50[Refereed]
- 3-Phenyllactic acid (PhLA) is useful as a start-up material in the pharmaceutical and biorefinery industries. To enhance the production of PhLA from glucose using recombinant Escherichia coli, the effects of glucose concentration and oxygen limitation on PhLA production are assessed in a fed-batch system using dissolved oxygen (DO)-stat method. The highest titer of PhLA (7.3 g L-1 ) is observed with a high concentration of glucose and under oxygen-limited conditions (DO = 0 ppm). Under oxygen limitation, cell growth and the formation of acetate and l-phenylalanine (Phe) by-products after 72 h of cultivation are reduced by 30%, 70%, and 81%, respectively, as compared to that under high DO conditions (DO = 5 ppm). Gene expression levels are compared between low and high DO conditions by quantitative polymerase chain reaction (qPCR) analysis. Several genes in the glycolysis (gapA and pykA), pentose phosphate (tktA), and early shikimate pathways for PhLA biosynthesis (aroF, aroG, and aroH) are upregulated under oxygen limitation. The results suggest that oxygen limitation affects metabolism in the shikimate pathway at both metabolic and transcriptional levels and that controlling the DO level is critical for enhanced production of a variety of aromatic compounds through the shikimate pathway.Jun. 2019, Biotechnology journal, 14(6) (6), e1800478, English, International magazine[Refereed]Scientific journal
- Biorefinery has been suggested to provide relevant substitutes to a number of fossil products. Feedstocks and conversion technologies have, however, been the bottleneck to the realization of this concept. Herein, feedstocks and bioconversion technologies under biorefinery have been reviewed. Over the last decade, research has shown possibilities of generating tens of new products but only few industrial implementations. This is partly associated with low production yields and poor cost-competitiveness. This review addresses the technical barriers associated with the conversion of emerging feedstocks into chemicals and bioenergy platforms and summarizes the developed biotechnological approaches including advances in metabolic engineering. This summary further suggests possible future advances that would expand the portfolio of biorefinery and speed up the realization of biofuels and biochemicals.Jun. 2019, Biotechnology journal, 14(6) (6), e1800494, English, International magazine[Refereed]Scientific journal
- Jun. 2019, Journal of bioscience and bioengineering, 127(6) (6), 726 - 731, English, Domestic magazine[Refereed]Scientific journal
- May 2019, Nature communications, 10(1) (1), 2040 - 2040, English, International magazineScientific journal
- Apr. 2019, GREEN CHEMISTRY, 21(7) (7), 1795 - 1808, English[Refereed]Scientific journal
- Mar. 2019, ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 7(5) (5), 5010 - 5017, English[Refereed]Scientific journal
- Aspergillus oryzae, a filamentous fungus, can secrete large amounts of enzymes extracellularly. We constructed a genetically engineered A. oryzae that simultaneously produced cellobiohydrolase, endoglucanase, and β-glucosidase by integrating multiple copies of the genes encoding these cellulases into fungal chromosomes. The resulting strain possessed 5-16 copies of each cellulase gene within the chromosome and showed approximately 10-fold higher activity versus single integration strains. Copy number polymorphisms were attributed to differences in flanking region sequence for the integrated gene fragments. Furthermore, we found that the P-sodM/T-glaB set demonstrated the strongest transcription levels per gene copy number. We therefore modified promoter/terminator set and cellulase gene combinations based on this polymorphism and transcription level data, with the resulting transformant showing 40-fold higher cellulolytic activity versus the single integration strain. This designed expression method could be useful for the overexpression of multiple enzymes and pathway flux control-mediated metabolic engineering in A. oryzae.Mar. 2019, Bioresource technology, 276, 146 - 153, English, International magazine[Refereed]Scientific journal
- Actinobacteria plays a key role in the cycling of organic matter in soils. They secret biomass-degrading enzymes that allow it to produce the unique metabolites that originate in plant biomass. Although past studies have focused on these unique metabolites, a large-scale screening of Actinobacteria is yet to be reported to focus on their biomass-degrading ability. In the present study, a rapid and simple method is constructed for a large-scale screening, and the novel resources that form the plant biomass-degrading enzyme cocktail are identified from 850 isolates of Actinobacteria. As a result, Nonomuraea fastidiosa secretes a biomass degrading enzyme cocktail with the highest enzyme titer, although cellulase activities are lower than a commercially available enzyme. So the rich accessory enzymes are suggested to contribute to the high enzyme titer for a pretreated bagasse with a synergistic effect. Additionally, an optimized cultivation method of biomass induction caused to produce the improved enzyme cocktail indicated strong enzyme titers and a strong synergistic effect. Therefore, the novel enzyme cocktails are selected via the optimized method for large-scale screening, and then the enzyme cocktail can be improved via the optimized production with biomass-induction.Mar. 2019, Biotechnology journal, 14(3) (3), e1700744, English, International magazine[Refereed]Scientific journal
- Feb. 2019, Biochemical Engineering Journal, 142, 117 - 123, English5-Hydroxymethylfurfural production from salt-induced photoautotrophically cultivated Chlorella sorokiniana[Refereed]Scientific journal
- A novel strategy for the low-cost, high-yield co-production of xylose and xylooligosaccharides together with no xylose inhibition was developed using a novel heterologous expression of XYN10Ks_480 endo-1,4-β-xylanase with a ricin-type β-trefoil type of domain and XYN11Ks_480 endo-1,4-β-xylanase with a CBM 2 superfamily from the Kitasatospora sp in an actinomycetes expression system. Xylose is the main building block for hemicellulose xylan. Our findings demonstrated high levels of expression and catalytic activity for XYN10Ks_480 during hydrolysis of the extracted xylan of bagasse, and three types of xylan-based substrates were used to produce xylose and xylooligosaccharides. However, hydrolysis by XYN11Ks_480 produced xylooligosaccharides without xylose formation. This study demonstrated how integrating sodium hypochlorite-extracted xylan and enzymatic hydrolysis could provide an alternative strategy for the generation of XOS from lignocellulosic material.Jan. 2019, Bioresource technology, 272, 315 - 325, English, International magazine[Refereed]Scientific journal
- 2019, Applied Nanoscience (Switzerland), 1 - 6, English[Refereed]Scientific journal
- American Chemical Society, Nov. 2018, Industrial and Engineering Chemistry Research, 57(44) (44), 14870 - 14877, English[Refereed]Scientific journal
- The aim of this study was to construct a cost-effective method for repeated bioethanol production using membrane (ultrafiltration permeation and nanofiltration concentration)-concentrated sweet sorghum juice by using flocculent Saccharomyces cerevisiae F118 strain. With low initial dry cell concentrations at around 0.28-0.35 g L-1, the S. cerevisiae F118 strain provided an ethanol titer of 86.19 ± 1.15 g L-1 (theoretical ethanol yield of 70.77%), which was higher than the non-flocculent S. cerevisiae BY4741 strain at 33.92 ± 0.99 g L-1 after 24 h fermentation. This result was correlated with higher gene expressions of the sucrose-hydrolysing enzyme invertase, sugar phosphorylation, and pyruvate-to-ethanol pathways in the F118 strain compared with the BY4741 strain. Sequential fed-batch fermentation was conducted, and the F118 strain was easily separated from the fermentation broth via the formation of flocs and sediment. After the 5th cycle of fermentation with the F118 strain, the ethanol concentration reached 100.37 g L-1.Oct. 2018, Bioresource technology, 265, 542 - 547, English, International magazine[Refereed]Scientific journal
- BACKGROUND: Genetic tools including constitutive and inducible promoters have been developed over the last few decades for strain engineering in Streptomyces. Inducible promoters are useful for controlling gene expression, however only a limited number are applicable to Streptomyces. The aim of this study is to develop a controllable protein expression system based on an inducible promoter using sugar inducer, which has not yet been widely applied in Streptomyces. RESULTS: To determine a candidate promoter, inducible protein expression was first examined in Streptomyces avermitilis MA-4680 using various carbon sources. Xylose isomerase (xylA) promoter derived from xylose (xyl) operon was selected due to strong expression of xylose isomerase (XylA) in the presence of D-xylose. Next, a xylose-inducible protein expression system was constructed by investigating heterologous protein expression (chitobiase as a model protein) driven by the xylA promoter in Streptomyces lividans. Chitobiase activity was detected at high levels in S. lividans strain harboring an expression vector with xylA promoter (pXC), under both xylose-induced and non-induced conditions. Thus, S. avermitilis xylR gene, which encodes a putative repressor of xyl operon, was introduced into constructed vectors in order to control protein expression by D-xylose. Among strains constructed in the study, XCPR strain harboring pXCPR vector exhibited strict regulation of protein expression. Chitobiase activity in the XCPR strain was observed to be 24 times higher under xylose-induced conditions than that under non-induced conditions. CONCLUSION: In this study, a strictly regulated protein expression system was developed based on a xylose-induced system. As far as we could ascertain, this is the first report of engineered inducible protein expression in Streptomyces by means of a xylose-induced system. This system might be applicable for controllable expression of toxic products or in the field of synthetic biology using Streptomyces strains.Sep. 2018, Microbial cell factories, 17(1) (1), 151 - 151, English, International magazine[Refereed]Scientific journal
- Elsevier B.V., Sep. 2018, Biochemical Engineering Journal, 137, 182 - 191, English[Refereed]Scientific journal
- (公社)日本生物工学会, Aug. 2018, 日本生物工学会大会講演要旨集, 平成30年度, 113 - 113, Japanese油脂酵母Lipomyces starkeyiD35株の繰り返し発酵による脂肪酸組成変化解析
- Hepatitis B virus core (HBc) particles acquire the capacity to disassemble and reassemble in a controlled manner, allowing entrapment and delivery of drugs and macromolecules to cells. HBc particles are made of 180-240 copies of 21 kDa protein monomers, assembled into 30-34 nm diameter icosahedral particles. In this study, we aimed at formulating HBc particles for the delivery of siRNA for gene silencing in vitro and in vivo. We have previously reported recombinant HBc particles expressing ZHER2 affibodies, specifically targeting human epidermal growth receptor 2 (HER2)-expressing cancer cells (ZHER2-ΔHBc). siRNA was encapsulated within the ZHER2-ΔHBc particles following disassembly and reassembly. The ZHER2-ΔHBc-siRNA hybrids were able to secure the encapsulated siRNA from serum and nucleases in vitro. Enhanced siRNA uptake in HER2-expressing cancer cells treated with ZHER2-ΔHBc-siRNA hybrids was observed compared to the nontargeted HBc-siRNA hybrids in a time- and dose-dependent manner. A successful in vitro polo-like kinase 1 (PLK1) gene knockdown was demonstrated in cancer cells treated with ZHER2-ΔHBc-siPLK1 hybrids, to levels comparable to commercial transfecting reagents. Interestingly, ZHER2-ΔHBc particles exhibit intrinsic capability of reducing the solid tumor mass, independent of siPLK1 therapy, in an intraperitoneal tumor model following intraperitoneal injection.Jul. 2018, ACS applied nano materials, 1(7) (7), 3269 - 3282, English, International magazineScientific journal
- Ultraviolet-absorbing chemicals are useful in cosmetics and skin care to prevent UV-induced skin damage. We demonstrate here that heterologous production of shinorine, which shows broad absorption maxima in the UV-A and UV-B region. A shinorine producing Corynebacterium glutamicum strain was constructed by expressing four genes from Actinosynnema mirum DSM 43827, which are responsible for the biosynthesis of shinorine from sedoheptulose-7-phosphate in the pentose phosphate pathway. Deletion of transaldolase encoding gene improved shinorine production by 5.2-fold. Among the other genes in pentose phosphate pathway, overexpression of 6-phosphogluconate dehydrogenase encoding gene further increased shinorine production by 60% (19.1 mg/L). The genetic engineering of the pentose phosphate pathway in C. glutamicum improved shinorine production by 8.3-fold in total, and could be applied to produce the other chemicals derived from sedoheptulose-7-phosphate.Jul. 2018, Bioscience, biotechnology, and biochemistry, 82(7) (7), 1252 - 1259, English, International magazine[Refereed]Scientific journal
- Polyacrylic acid (PAA)-modified titanium peroxide nanoparticles (PAA-TiOx NPs) are promising radiosensitizers. PAA-TiOx NPs were synthesized from commercial TiO2 nanoparticles that were modified with PAA and functionalized by H2O2 treatment. To realize practical clinical uses for PAA-TiOx NPs, their tissue distribution and acute toxicity were evaluated using healthy mice and mice bearing tumors derived from xenografted MIAPaCa-2 human pancreatic cancer cells. Healthy mice were injected with PAA-TiOx NPs at 25 mg/kg body weight via the tail vein, and tumor-bearing mice were injected either into the tumor locally or via the tail vein. The concentration of PAA-TiOx NPs in major organs was determined over time using inductively coupled-plasma atomic emission spectrometry. After 1 h, 12% of the PAA-TiOx NP dose had accumulated in the tumor, and 2.8% of the dose remained after 1 week. Such high accumulation could be associated with enhanced permeability and retention effects of the tumor, as PAA-TiOx NPs are composed of inorganic particles and polymers, without tumor-targeting molecules. The liver accumulated the largest proportion of the injected nanoparticles, up to 42% in tumor-bearing mice. Blood biochemical parameters were also investigated after intravenous injection of PAA-TiOx NPs in healthy mice. PAA-TiOx NPs invoked a slight change in various liver-related biochemical parameters, but no liver injury was observed over the practical dose range. In the future, PAA-TiOx NPs should be modified to prevent accumulation in the liver and minimize risk to patients.Jul. 2018, Journal of bioscience and bioengineering, 126(1) (1), 119 - 125, English, Domestic magazine[Refereed]Scientific journal
- Elsevier B.V., Jun. 2018, Journal of Bioscience and Bioengineering, 125(6) (6), 695 - 702, English[Refereed]Scientific journal
- BioMed Central Ltd., May 2018, Microbial cell factories, 17(1) (1), 76 - 76, English, International magazine[Refereed]Scientific journal
- 日本DDS学会, May 2018, 日本DDS学会学術集会プログラム予稿集, 34回, 207 - 207, Japanese新規放射線増感剤としての過酸化チタンナノ粒子の物性解析
- Mar. 2018, Bioresource Technology, 252, 157 - 164, English[Refereed]Scientific journal
- Elsevier B.V., Feb. 2018, Chemical Engineering Journal, 334, 657 - 663, English[Refereed]Scientific journal
- Feb. 2018, CARBOHYDRATE POLYMERS, 182, 8 - 14, English[Refereed]Scientific journal
- The Japan Institute of Energy, 2018, Proceedings of the Conference on Biomass Science, 13(0) (0), 135 - 136, Japanese
Caffeic acid (3,4-dihydroxycinnamic acid) serves as a building block for thermoplastics and was recently produced from glucose by microbial fermentation. To produce caffeic acid from inedible cellulose, separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) reactions were compared using kraft pulp as lignocellulosic feedstock. Here, a tyrosine-overproducing Escherichia coli strain was metabolically engineered to produce caffeic acid from glucose by expressing the genes encoding a tyrosine ammonia lyase (fevV) from Streptomyces sp. WK-5344 and 4-hydroxyphenyllactate 3-hydroxylase (hpaBC) from Pseudomonas aeruginosa. Using the resulting recombinant strain, the maximum yield of caffeic acid in SSF (233 mg/L) far exceeded that by SHF (37.9 mg/L). In the SSF with low cellulase loads (≤2.5 filter paper unit/g glucan), caffeic acid production was markedly increased, while almost no glucose accumulation was detected, indicating that the E. coli cells experienced glucose limitation in this culture condition. Caffeic acid yield was also negatively correlated with the glucose concentration in the fermentation medium. These observations suggest that glucose limitation in SSF is crucial for improving caffeic acid yield, owing to reduced by-product formation and fermentation inhibitor accumulation.
[Refereed] - Elsevier B.V., Jan. 2018, Industrial Crops and Products, 111, 457 - 461, EnglishScientific journal
- Japan Society for Bioscience Biotechnology and Agrochemistry, 2018, Bioscience, Biotechnology and Biochemistry, 82(5) (5), 904 - 915, English[Refereed]Scientific journal
- Society of Chemical Engineers, Japan, 2018, Journal of Chemical Engineering of Japan, 51(2) (2), 143 - 151, English[Refereed]Scientific journal
- 日本遺伝学会, 2018, Genes and Genetic Systems, 93(1) (1), 1 - 7, English[Refereed]Scientific journal
- BioMed Central Ltd., 2018, Biotechnology for biofuels, 11(1) (1), 50 - 50, English, International magazine[Refereed]Scientific journal
- Measuring the concentrations of metabolites and estimating the reaction rates of each reaction step consisting of metabolic pathways are significant for an improvement in microorganisms used in maximizing the production of materials. Although the reaction pathway must be identified for such an improvement, doing so is not easy. Numerous reaction steps have been reported; however, the actual reaction steps activated vary or change according to the conditions. Furthermore, to build mathematical models for a dynamical analysis, the reaction mechanisms and parameter values must be known; however, to date, sufficient information has yet to be published for many cases. In addition, experimental observations are expensive. A new mathematical approach that is applicable to small sample data, and that requires no detailed reaction information, is strongly needed. S-system is one such model that can use smaller samples than other ordinary differential equation models. We propose a simplified S-system to apply minimal quantities of samples for a dynamic analysis of the metabolic pathways. We applied the model to the phenyl lactate production pathway of Escherichia coli. The model obtained suggests that actually activated reaction steps and feedback are inhibitions within the pathway.2018, Bioinformatics and biology insights, 12, 1177932218775076 - 1177932218775076, English, International magazine[Refereed]Scientific journal
- 2018, Genes and Genetic Systems, 93(1) (1), 1 - 7, English[Refereed]Scientific journal
- Ferulic acid (4-hydroxy-3-methoxycinnamic acid, FA) is a lignin-derived phenolic compound abundant in plant biomass. The utilization of FA and its conversion to valuable compounds is desired. Protocatechuic acid (3,4-dihydroxybenzoic acid, PCA) is a precursor of polymers and plastics and a constituent of food. A microbial conversion system to produce PCA from FA was developed in this study using a PCA-producing strain of Corynebacterium glutamicum F (ATCC 21420). C. glutamicum strain F grown at 30 °C for 48 h utilized 2 mM each of FA and vanillic acid (4-hydroxy-3-methoxybenzoic acid, VA) to produce PCA, which was secreted into the medium. FA may be catabolized by C. glutamicum through proposed (I) non-β-oxidative, CoA-dependent or (II) β-oxidative, CoA-dependent phenylpropanoid pathways. The conversion of VA to PCA is the last step in each pathway. Therefore, the vanillate O-demethylase gene (vanAB) from Corynebacterium efficiens NBRC 100395 was expressed in C. glutamicum F (designated strain FVan) cultured at 30 °C in AF medium containing FA. Strain C. glutamicum FVan converted 4.57 ± 0.07 mM of FA into 2.87 ± 0.01 mM PCA after 48 h with yields of 62.8% (mol/mol), and 6.91 mM (1064 mg/L) of PCA was produced from 16.0 mM of FA after 12 h of fed-batch biotransformation. Genomic analysis of C. glutamicum ATCC 21420 revealed that the PCA-utilization genes (pca cluster) were conserved in strain ATCC 21420 and that mutations were present in the PCA importer gene pcaK.Dec. 2017, AMB Express, 7(1) (1), 130 - 130, English, International magazine[Refereed]Scientific journal
- Dec. 2017, BIORESOURCE TECHNOLOGY, 245, 1655 - 1663, English[Refereed]Scientific journal
- Dec. 2017, BIORESOURCE TECHNOLOGY, 245, 1664 - 1673, English[Refereed]Scientific journal
- Dec. 2017, BIORESOURCE TECHNOLOGY, 245, 1314 - 1326, English[Refereed]Scientific journal
- Dec. 2017, BIORESOURCE TECHNOLOGY, 245, 1413 - 1420, English[Refereed]Scientific journal
- Dec. 2017, BIORESOURCE TECHNOLOGY, 245, 1436 - 1446, English[Refereed]Scientific journal
- Dec. 2017, INDUSTRIAL CROPS AND PRODUCTS, 109, 158 - 162, English[Refereed]Scientific journal
- Dec. 2017, BIORESOURCE TECHNOLOGY, 245, 1400 - 1406, English[Refereed]Scientific journal
- Dec. 2017, ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 28, 16 - 23, English[Refereed]Scientific journal
- John Wiley and Sons Inc., Nov. 2017, Journal of Biomedical Materials Research - Part B Applied Biomaterials, 105(8) (8), 2344 - 2351, English[Refereed]Scientific journal
- Nov. 2017, SENSORS AND ACTUATORS B-CHEMICAL, 252, 813 - 821, English[Refereed]Scientific journal
- Nov. 2017, SENSORS AND ACTUATORS B-CHEMICAL, 252, 600 - 605, English[Refereed]Scientific journal
- The juice from sweet sorghum cultivar SIL-05 (harvested at physiological maturity) was extracted, and the component sucrose and reducing sugars (such as glucose and fructose) were subjected to a membrane separation process to purify the sucrose for subsequent sugar refining and to obtain a feedstock for repeated bioethanol production. Nanofiltration (NF) of an ultrafiltration (UF) permeate using an NTR-7450 membrane (Nitto Denko Corporation, Osaka, Japan) concentrated the juice and produced a sucrose-rich fraction (143.2 g L-1 sucrose, 8.5 g L-1 glucose, and 4.5 g L-1 fructose). In addition, the above NF permeate was concentrated using an ESNA3 NF membrane to provide concentrated permeated sugars (227.9 g L-1) and capture various amino acids in the juice, enabling subsequent ethanol fermentation without the addition of an exogenous nitrogen source. Sequential batch fermentation using the ESNA3 membrane concentrate provided an ethanol titer and theoretical ethanol yield of 102.5-109.5 g L-1 and 84.4-89.6%, respectively, throughout the five-cycle batch fermentation by Saccharomyces cerevisiae BY4741. Our results demonstrate that a membrane process using UF and two types of NF membranes has the potential to allow sucrose purification and repeated bioethanol production.Aug. 2017, Applied microbiology and biotechnology, 101(15) (15), 6007 - 6014, English, International magazine[Refereed]Scientific journal
- Jul. 2017, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 101(13) (13), 5279 - 5290, English[Refereed]Scientific journal
- May 2017, APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 182(1) (1), 229 - 237, English[Refereed]Scientific journal
- May 2017, AMB EXPRESS, 7, English[Refereed]Scientific journal
- Mar. 2017, SCIENTIFIC REPORTS, 7, 43160, English[Refereed]Scientific journal
- Mar. 2017, BIOTECHNOLOGY JOURNAL, 12(3) (3), 1600400, English[Refereed]Scientific journal
- Mar. 2017, COLLOIDS AND SURFACES B-BIOINTERFACES, 151, 134 - 142, English[Refereed]Scientific journal
- Mar. 2017, BIOMATERIALS, 120, 126 - 138, English[Refereed]Scientific journal
- Feb. 2017, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 27(4) (4), 954 - 957, English[Refereed]Scientific journal
- Jan. 2017, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 123(1) (1), 101 - 108, English[Refereed]Scientific journal
- 2017, Journal of Bioprocessing & Biotechniques, 7(2) (2), 300: 1 - 5, English[Refereed]Scientific journal
- Jan. 2017, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 27(2) (2), 336 - 341, English[Refereed]Scientific journal
- 2017, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 81(8) (8), 1650 - 1656, English[Refereed]Scientific journal
- 2017, PLANT PRODUCTION SCIENCE, 20(4) (4), 441 - 447, English[Refereed]Scientific journal
- Dec. 2016, INDUSTRIAL CROPS AND PRODUCTS, 94, 308 - 317, English[Refereed]Scientific journal
- Dec. 2016, FREE RADICAL RESEARCH, 50(12) (12), 1319 - 1328, English[Refereed]Scientific journal
- Dec. 2016, CURRENT OPINION IN BIOTECHNOLOGY, 42, 30 - 39, English[Refereed]Scientific journal
- Nov. 2016, BIOPROCESS AND BIOSYSTEMS ENGINEERING, 39(11) (11), 1793 - 1801, English[Refereed]Scientific journal
- Bentham Science Publishers B.V., Oct. 2016, Open Chemical Engineering Journal, 10, 88 - 109, English[Refereed]Scientific journal
- Sep. 2016, Proceedings of 5th Asian Conference on Mixing, 2016 (ACOM2016), 45 - 50, EnglishEffect of Swingstir impeller on rheology and morphology of submerged fermentation[Refereed]International conference proceedings
- Sep. 2016, BIOTECHNOLOGY FOR BIOFUELS, 9(1) (1), 188, English[Refereed]Scientific journal
- Sep. 2016, MICROBIAL CELL FACTORIES, 15(1) (1), 155, English[Refereed]Scientific journal
- Jul. 2016, BIORESOURCE TECHNOLOGY, 211, 224 - 230, English[Refereed]Scientific journal
- Jul. 2016, RADIATION ONCOLOGY, 11(1) (1), 91, English[Refereed]Scientific journal
- Elsevier, Jun. 2016, Ultrasonics Sonochemistry, 28, 1 - 6, EnglishScientific journal
- May 2016, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 80(5) (5), 863 - 869, English[Refereed]Scientific journal
- Apr. 2016, SCIENTIFIC REPORTS, 6, 24550, English[Refereed]Scientific journal
- Feb. 2016, BIOTECHNOLOGY FOR BIOFUELS, 9, 27, English[Refereed]Scientific journal
- Feb. 2016, CELLULOSE, 23(1) (1), 403 - 414, English[Refereed]Scientific journal
- Hosokawa Powder Technology Foundation, 2016, Hosokawa Powder Technology Foundation ANNUAL REPORT, 24, 30 - 34, Japanese
Radiosensizing therapy, combining radiotherapy and an agent for amplifying radiation, have an advantage for therapy of low-oxygen cancer including pancreatic. In our recent study, the hydrogen peroxide treatment of titanium dioxide nanoparticles induced titanium peroxide nanoparticle (PAATiOx), and it was suggested to use as radiosensitizing agent for radiotherapy. By this combination, the apparent therapeutic positive effect was observed in vivo study of human pancreatic xenograft mice. However, it can't be said that it has a high usefulness for radiosensizing therapy. So we prepared antibody modification PAATiOx (PAATiOx/1a), the antibody is the variable domain of a camelid heavy chain-only antibody (1a), which has high affinity to EGFR, expressed in various cancer cells. As a result, PAATiOx/1a with X-ray treatment showed more cytotoxicity than PAATiOx with equivalent dose of X-ray irradiation in vitro and in vivo. Since this study showed that PAATiOx/1a could target EGFR-expressing cells, we would use this particle as a drug delivery carrier for various cancer cells expressing EGFR.
- Protocatechuic acid (3,4-dihydroxybenzoic acid; PCA) serves as a building block for polymers and pharmaceuticals. In this study, the biosynthetic pathway for PCA from glucose was engineered in Corynebacterium glutamicum. The pathway to PCA-employed elements of the chorismate pathway by using chorismate-pyruvate lyase (CPL) and 4-hydroxybenzoate hydroxylase (4-HBA hydroxylase). As C. glutamicum has the potential to synthesize the aromatic amino acid intermediate chorismate and possesses 4-HBA hydroxylase, we focused on expressing Escherichia coli CPL in a phenylalanine-producing strain of C. glutamicum ATCC21420. To secrete PCA, the gene (ubiC) encoding CPL from E. coli was expressed in C. glutamicum ATCC 21420 (strain F(UbiC)). The formation of 28.8 mg/L of extracellular 4-HBA (36 h) and 213 ± 29 mg/L of extracellular PCA (80 h) was obtained by the C. glutamicum strain F(UbiC) from glucose. The strain ATCC21420 was also found to produce extracellular PCA. PCA fermentation was performed using C. glutamicum strain F(UbiC) in a bioreactor at the optimized pH of 7.5. C. glutamicum F(UbiC) produced 615 ± 2.1 mg/L of PCA from 50 g/L of glucose after 72 h. Further, fed-batch fermentation of PCA by C. glutamicum F(UbiC) was performed with feedings of glucose every 24 h. The maximum production of PCA (1140.0 ± 11.6 mg/L) was achieved when 117.0 g/L of glucose was added over 96 h of fed-batch fermentation.Jan. 2016, Applied microbiology and biotechnology, 100(1) (1), 135 - 45, English, International magazine[Refereed]
- Sorghum bagasse pretreated with diluted acid, which was predominantly composed of glucan (59%) and xylan (7.2%), was used as a lignocellulosic feedstock to produce D-phenyllactic acid (PhLA) by a recombinant Escherichia coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens. Compared to filter paper hydrolysate, the PhLA yield was reduced by 35% during fermentation with enzymatic hydrolysate of sorghum bagasse as a carbon source, and metabolomics analysis revealed that intracellular levels of erythrose-4-phosphate and phosphoenolpyruvate and NAD(P)H regeneration for PhLA production from glucose markedly reduced. Compared to the separate hydrolysis and fermentation (SHF) with sorghum bagasse hydrolysate, simultaneous saccharification and fermentation (SSF) of sorghum bagasse under glucose limitation conditions yielded 4.8-fold more PhLA with less accumulation of eluted components of p-coumaric acid and aldehydes, which inhibited PhLA fermentation. These results suggest that gradual hydrolysis of sorghum bagasse during SSF reduces the accumulation of both glucose and fermentation inhibitors, collectively leading to increased PhLA yield.The Japan Institute of Energy, Jan. 2016, Proceedings of the Conference on Biomass Science, 11, 35 - 36, Japanese[Refereed]International conference proceedings
- Jan. 2016, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 121(1) (1), 96 - 100, English[Refereed]Scientific journal
- Jan. 2016, BIOCHEMICAL ENGINEERING JOURNAL, 105, 10 - 15, English[Refereed]Scientific journal
- Dec. 2015, BIORESOURCE TECHNOLOGY, 198, 410 - 417, English[Refereed]Scientific journal
- Elsevier, Nov. 2015, Biochemical Engineering Journal, 103, 198 - 204, EnglishScientific journal
- Oct. 2015, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 99(19) (19), 7879 - 7891, English[Refereed]Scientific journal
- Oct. 2015, 化学と生物, 53(10) (10), 689 - 695, Japaneseバイオリファイナリーの現状と展望Scientific journal
- Aug. 2015, Bioresource Technology, 189, 203 - 209, English[Refereed]Scientific journal
- Jul. 2015, BIORESOURCE TECHNOLOGY, 187, 167 - 172, English[Refereed]Scientific journal
- Jun. 2015, PLOS ONE, 10(6) (6), e0128417, English[Refereed]Scientific journal
- Jun. 2015, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 79(6) (6), 1034 - 1037, English[Refereed]Scientific journal
- Jun. 2015, BIORESOURCE TECHNOLOGY, 186, 351 - 355, English[Refereed]Scientific journal
- Jun. 2015, BIOTECHNOLOGY FOR BIOFUELS, 8, 88, English[Refereed]Scientific journal
- Jun. 2015, BIORESOURCE TECHNOLOGY, 185, 263 - 268, English[Refereed]Scientific journal
- (一社)日本放射線影響学会, May 2015, 日本放射線影響学会大会講演要旨集, 58回, 3 - 07, English新たな放射線増感剤としての過酸化チタンナノ粒子は膵癌治療における抗腫瘍効果を増強させる(Titanium Peroxide Nanoparticles, as Novel Radiosensitizers, Enhance Antitumor Efficacy in Pancreatic Cancer Therapy)
- Apr. 2015, BIORESOURCE TECHNOLOGY, 182, 169 - 178, English[Refereed][Invited]Scientific journal
- Feb. 2015, JOURNAL OF NANOBIOTECHNOLOGY, 13(1) (1), 15, English[Refereed]Scientific journal
- Springer International Publishing, Jan. 2015, Green Processes for Nanotechnology: From Inorganic to Bioinspired Nanomaterials, 237 - 257, English[Refereed]In book
- 2015, Bioresourse Technology, 6, 888, EnglishSaccharification and ethanol fermentation from cholinium ionic liquid-pretreated bagasse with a different number of post-pretreatment washings[Refereed]Scientific journal
- Jan. 2015, BIORESOURCE TECHNOLOGY, 176, 169 - 174, English[Refereed]Scientific journal
- 2015, Biogenic Approach, Springer, 237 - 257, JapaneseGreen Processes for Nanotechnology, Chapter 8, Nanoparticle SynthesisScientific journal
- Jan. 2015, BIOTECHNOLOGY LETTERS, 37(1) (1), 89 - 94, English[Refereed]Scientific journal
- Jan. 2015, CHEMICAL ENGINEERING JOURNAL, 259, 323 - 329, English[Refereed]Scientific journal
- Jan. 2015, 化学と生物, 53(1) (1), 9 - 11, Japanese二酸化チタン粒子の生物学的応用Scientific journal
- Jan. 2015, BIOTECHNOLOGY LETTERS, 37(1) (1), 89 - 94, English[Refereed]Scientific journal
- 2015, NANOSCALE, 7(11) (11), 4956 - 4963, English[Refereed]Scientific journal
- Dec. 2014, BIORESOURCE TECHNOLOGY, 173, 376 - 383, English[Refereed]Scientific journal
- Dec. 2014, MICROBIAL CELL FACTORIES, 13(1) (1), 175, English[Refereed]Scientific journal
- Nov. 2014, BIOTECHNOLOGY LETTERS, 36(11) (11), 2311 - 2317, English[Refereed]Scientific journal
- Oct. 2014, BIORESOURCE TECHNOLOGY, 169, 380 - 386, English[Refereed]Scientific journal
- Oct. 2014, BIORESOURCE TECHNOLOGY, 169, 821 - 825, English[Refereed]Scientific journal
- (公社)日本分析化学会, Sep. 2014, 日本分析化学会講演要旨集, 63年会, 168 - 168, Japaneseラジカルを発生する金属酸化物ナノ粒子のX線を用いた分析
- Sep. 2014, ULTRASONICS SONOCHEMISTRY, 21(5) (5), 1624 - 1628, English[Refereed]Scientific journal
- Sep. 2014, BIOCHEMICAL ENGINEERING JOURNAL, 90, 90 - 95, English[Refereed]Scientific journal
- Sep. 2014, JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 10(9) (9), 2063 - 2085, English[Refereed]Scientific journal
- Aug. 2014, BIOTECHNOLOGY FOR BIOFUELS, 7, 120, English[Refereed]Scientific journal
- Jul. 2014, PROCESS BIOCHEMISTRY, 49(7) (7), 1078 - 1083, English[Refereed]Scientific journal
- Jul. 2014, BIOCHEMICAL ENGINEERING JOURNAL, 88, 188 - 194, English[Refereed]Scientific journal
- テックタイムス, Jun. 2014, 紙パルプ技術タイムス, 57(6) (6), 51 - 56, Japanese「バイオプロダクション次世代農工連携研究」と拠点形成への展望Scientific journal
- Jun. 2014, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 104, 17 - 22, English[Refereed]Scientific journal
- May 2014, Ultrasonic Sonochemistry, 21(1) (1), 289 - 294, EnglishEnhanced OH radical generation by dual-frequency ultrasound with TiO2nanoparticles: its appplication to targeted sonodynamic therapy[Refereed]Scientific journal
- May 2014, MICROBIAL CELL FACTORIES, 13, 71, English[Refereed]Scientific journal
- シーエムシー出版, Apr. 2014, バイオインダストリー, 31(4) (4), 62 - 68, Japanese植物の機能解明と新たな機能材料創出の可能性Scientific journal
- Apr. 2014, AMB EXPRESS, 4, 20, English[Refereed]Scientific journal
- Apr. 2014, JOURNAL OF NANOBIOTECHNOLOGY, 12(1) (1), 11, English[Refereed]Scientific journal
- Feb. 2014, Nucleosides, Nucleotides and Nucleic Acids, 33(1) (1), 31 - 39, EnglishStructural evaluation of the DNA aptamer for ATP DH25.42 by AFM., Nucleosides[Refereed]Scientific journal
- Feb. 2014, ANALYTICAL BIOCHEMISTRY, 446, 37 - 43, English[Refereed]Scientific journal
- 2014, RSC ADVANCES, 4(12) (12), 5986 - 5989, English[Refereed]Scientific journal
- 2014, RSC ADVANCES, 4(89) (89), 48458 - 48464, English[Refereed]Scientific journal
- Jan. 2014, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 117(1) (1), 129 - 133, English[Refereed]Scientific journal
- Dec. 2013, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 77(12) (12), 2449 - 2453, English[Refereed]Scientific journal
- Dec. 2013, BIORESOURCE TECHNOLOGY, 149, 520 - 524, English[Refereed]Scientific journal
- Nov. 2013, RSC Advances, 3(42) (42), 19268 - 19271[Refereed]Scientific journal
- Nov. 2013, BIORESOURCE TECHNOLOGY, 147, 84 - 88, English[Refereed]Scientific journal
- 日本生物工学会, Aug. 2013, 日本生物工学会大会講演要旨集平成25年度, 65, 244 - 244, Japaneseナノ粒子とX線照射を併用した深部ガンの非侵襲的治療法の開発Research society
- Aug. 2013, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 116(2) (2), 214 - 218, English[Refereed]Scientific journal
- Aug. 2013, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(15) (15), 6749 - 6757, English[Refereed]Scientific journal
- Jun. 2013, JOURNAL OF NANOBIOTECHNOLOGY, 11, 19, English[Refereed]Scientific journal
- Jun. 2013, Microbial Cell Factories, 12(1) (1), 66, EnglishSynergetic effect of yeast cell-surface expression of cellulase and expansin-like protein on direct ethanol production from cellulose[Refereed]Scientific journal
- May 2013, Microbial Cell Factories, 12(1) (1), 45, English[Refereed]Scientific journal
- May 2013, Biochemical Engineering Journal, 74, 76 - 80, English[Refereed]Scientific journal
- May 2013, CARBON, 56, 309 - 316, English[Refereed]Scientific journal
- May 2013, BIORESOURCE TECHNOLOGY, 135, 410 - 416, English[Refereed]Scientific journal
- May 2013, BIORESOURCE TECHNOLOGY, 135, 103 - 108, English[Refereed]Scientific journal
- May 2013, ENZYME AND MICROBIAL TECHNOLOGY, 52(6-7) (6-7), 331 - 335, English[Refereed]Scientific journal
- Mar. 2013, ULTRASONICS SONOCHEMISTRY, 20(2) (2), 762 - 767, English[Refereed]Scientific journal
- Mar. 2013, JOURNAL OF BIOCHEMISTRY, 153(3) (3), 251 - 256, English[Refereed]Scientific journal
- Mar. 2013, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 23(6) (6), 1797 - 1802, English[Refereed]Scientific journal
- Mar. 2013, Journal of Virological Methods, 189(2) (2), 393 - 396, EnglishAn affinity chromatography method used to purify His-tag-displaying bio-nanocapsules[Refereed]Scientific journal
- Feb. 2013, Biochemical Engineering Journal, 71, 25 - 29, EnglishScientific journal
- Feb. 2013, 化学工業, 64(2) (2), 52 - 55, Japaneseイオン液体前処理バイオマスを用いたエタノール同時糖化発酵Scientific journal
- Feb. 2013, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 115(2) (2), 196 - 199, English[Refereed]Scientific journal
- Feb. 2013, NANOSCALE RESEARCH LETTERS, 8(1) (1), 70, English[Refereed]Scientific journal
- Feb. 2013, ENZYME AND MICROBIAL TECHNOLOGY, 52(2) (2), 118 - 122, English[Refereed]Scientific journal
- Jan. 2013, Biochemical Engineering Journal, 70, 135 - 139, English[Refereed]Scientific journal
- Jan. 2013, Chemical Engineering Journal, 215-216, 811 - 818, EnglishScientific journal
- 2013, RSC ADVANCES, 3(40) (40), 18367 - 18372, English[Refereed]Scientific journal
- Jan. 2013, BIORESOURCE TECHNOLOGY, 128, 188 - 192, English[Refereed]Scientific journal
- Springer Verlag, 2013, AMB Express, 3(1) (1), 1 - 7, English[Refereed]Scientific journal
- Dec. 2012, JOURNAL OF DRUG TARGETING, 20(10) (10), 897 - 905, English[Refereed]Scientific journal
- Oct. 2012, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 96(1) (1), 81 - 88, English[Refereed]Scientific journal
- Aug. 2012, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 114(2) (2), 209 - 211, English[Refereed]Scientific journal
- Aug. 2012, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 95(3) (3), 577 - 591, English[Refereed]Scientific journal
- Aug. 2012, ENZYME AND MICROBIAL TECHNOLOGY, 51(3) (3), 171 - 176, English[Refereed]Scientific journal
- Jul. 2012, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 114(1) (1), 80 - 85, English[Refereed]Scientific journal
- May 2012, Enzyme and Microbial Technology, 50(6-7) (6-7), 343 - 347, English[Refereed]Scientific journal
- May 2012, バイオインダストリー, 29, 38 - 45, Japaneseイオン液体と超音波照射を組み合わせたリグノセルロース前処理によるバイオマス・リファイナリーScientific journal
- May 2012, ULTRASONICS SONOCHEMISTRY, 19(3) (3), 607 - 614, English[Refereed]Scientific journal
- May 2012, JOURNAL OF BIOTECHNOLOGY, 159(1-2) (1-2), 32 - 37, English[Refereed]Scientific journal
- May 2012, PLOS ONE, 7(5) (5), e37136, English[Refereed]Scientific journal
- May 2012, BIOTECHNOLOGY JOURNAL, 7(5) (5), 686 - 689, English[Refereed]Scientific journal
- Apr. 2012, CANCER RESEARCH, 72, English[Refereed]
- Apr. 2012, CANCER RESEARCH, 72, English
- Apr. 2012, MICROBIAL CELL FACTORIES, 11(1) (1), 49, English[Refereed]Scientific journal
- 日本生物工学会, Mar. 2012, 生物工学会誌, 90(3) (3), 110 - 114, Japanese酸化チタン粒子のバイオ応用Scientific journal
- Feb. 2012, PROTEIN EXPRESSION AND PURIFICATION, 81(2) (2), 151 - 156, English[Refereed]Scientific journal
- Feb. 2012, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 93(4) (4), 1495 - 1502, English[Refereed]Scientific journal
- Jan. 2012, BIORESOURCE TECHNOLOGY, 103(1) (1), 446 - 452, English[Refereed]Scientific journal
- Jan. 2012, JOURNAL OF BIOTECHNOLOGY, 157(1) (1), 124 - 129, English[Refereed]Scientific journal
- Jan. 2012, BIORESOURCE TECHNOLOGY, 104, 648 - 651, English[Refereed]Scientific journal
- Nov. 2011, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 112(5) (5), 511 - 514, English[Refereed]Scientific journal
- Oct. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 92(1) (1), 67 - 76, English[Refereed]Scientific journal
- Sep. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 91(6) (6), 1537 - 1544, English[Refereed]Scientific journal
- Sep. 2011, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77(17) (17), 6265 - 6270, English[Refereed]Scientific journal
- Aug. 2011, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 75(8) (8), 1603 - 1605, English[Refereed]Scientific journal
- Aug. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 91(4) (4), 1001 - 1006, English[Refereed]Scientific journal
- Jul. 2011, ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 67, 779 - 781, English[Refereed]Scientific journal
- Jun. 2011, BIORESOURCE TECHNOLOGY, 102(12) (12), 6723 - 6729, English[Refereed]Scientific journal
- May 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 90(3) (3), 1171 - 1177, English[Refereed]Scientific journal
- May 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 90(3) (3), 895 - 901, English[Refereed]Scientific journal
- May 2011, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 38(5) (5), 643 - 648, English[Refereed]Scientific journal
- Apr. 2011, ENZYME AND MICROBIAL TECHNOLOGY, 48(4-5) (4-5), 393 - 396, English[Refereed]Scientific journal
- Mar. 2011, Biotechnology Letters, 33(4), 727-731, 727 - 731, EnglishExtracellular production of a sphingomyelinase fromStreptomyces griseocarneus using Streptomyces lividans[Refereed]Scientific journal
- Mar. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 89(5) (5), 1417 - 1422, English[Refereed]Scientific journal
- Our previous study suggested new sonodynamic therapy for cancer cells based on the delivery of titanium dioxide (TiO_2) nanoparticles (NPs) modified with a protein specifically recognizing target cells and subsequent generation of hydroxyl radicals from TiO_2 NPs activated by external ultrasound irradiation (called TiO_2/US treatment). The present study first examined the uptake behavior of TiO_2 NPs modified with pre-S1/S2 (model protein-recognizing hepatocytes) by HepG2 cells for 24 h. Next, the effect of the TiO_2/US treatment on HepG2 cell growth was examined for 96 h after the 1 MHz ultrasound was irradiated (0.1 W/cm^2, 30 s) to the cells which incorporated the TiO_2 NPs. Although no apparent cell-injury was observed until 24 h after the treatment, the viable cell concentration had deteriorated to 46% of the control at 96 h. Finally, the TiO_2/US treatment was applied to a mouse xenograft model. The pre-S1/S2-immobilized TiO_2 (0.1 mg) was directly injected into tumors, followed by 1 MHz ultrasound irradiation at 1.0 W/cm^2 for 60 s. As a result of the treatment repeated 5 times within 13 days, tumor growth could be hampered up to 28 days compared with the control conditions.The Japan Society of Sonochemistry, 2011, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 20, 80 - 83, English
- 2011, 2011 Defense Science Research Conference and Expo, DSR 2011, English[Refereed]International conference proceedings
- BioMed Central Ltd., 2011, Biotechnology for Biofuels, 4(1) (1), 8, English[Refereed]Scientific journal
- 2011, Biotechnology for Biofuels, 4:8, EnglishDirect ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiae with optimized cellulase expression[Refereed]Scientific journal
- 2011, GREEN CHEMISTRY, 13(10) (10), 2948 - 2953, English[Refereed]Scientific journal
- Nov. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 88(5) (5), 1215 - 1221, English[Refereed]Scientific journal
- Nov. 2010, Applied Microbiology and Biotechnology, 88, 1215-1221, EnglishConstruction of a xylose-metabolizing Saccharomyces cerevisiae by integration of xylose isomerase gene into the genome and investigation of the effect of xylITOl on fermentation[Refereed]Scientific journal
- Oct. 2010, PROTEIN EXPRESSION AND PURIFICATION, 73(2) (2), 198 - 202, English[Refereed]Scientific journal
- Oct. 2010, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 20(19) (19), 5726 - 5731, English[Refereed]Scientific journal
- Sep. 2010, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 66(1-2) (1-2), 101 - 104, English[Refereed]Scientific journal
- Sep. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 88(1) (1), 381 - 388, English[Refereed]Scientific journal
- Sep. 2010, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 20(17) (17), 5320 - 5325, English[Refereed]Scientific journal
- Sep. 2010, MEDCHEMCOMM, 1(3) (3), 209 - 211, English[Refereed]Scientific journal
- Aug. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(5) (5), 1783 - 1789, English[Refereed]Scientific journal
- Jun. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(1) (1), 109 - 115, English[Refereed]Scientific journal
- Jun. 2010, JOURNAL OF BIOCHEMISTRY, 147(6) (6), 867 - 873, English[Refereed]Scientific journal
- Jun. 2010, JOURNAL OF BIOCHEMISTRY, 147(6) (6), 875 - 884, English[Refereed]Scientific journal
- May 2010, BIOTECHNOLOGY JOURNAL, 5(5) (5), 449 - 455, English[Refereed]Scientific journal
- May 2010, MICROBIAL CELL FACTORIES, 9, English[Refereed]Scientific journal
- Apr. 2010, ULTRASONICS SONOCHEMISTRY, 17(4) (4), 738 - 743, English[Refereed]Scientific journal
- Mar. 2010, NUCLEIC ACIDS RESEARCH, 38(4) (4), English[Refereed]Scientific journal
- Mar. 2010, ENZYME AND MICROBIAL TECHNOLOGY, 46(3-4) (3-4), 194 - 199, English[Refereed]Scientific journal
- Feb. 2010, Biochemical Engineering Journal, 48(3) (3), 416 - 423, English[Refereed]Scientific journal
- Feb. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85(5) (5), 1491 - 1498, English[Refereed]Scientific journal
- 公益社団法人 化学工学会, 2010, 化学工学会 研究発表講演要旨集, 2010, 1008 - 1008, Japanese
- 公益社団法人 化学工学会, 2010, 化学工学会 研究発表講演要旨集, 2010, 983 - 983, Japanese
- 公益社団法人 化学工学会, 2010, 化学工学会 研究発表講演要旨集, 2010, 963 - 963, Japanese
- 公益社団法人 化学工学会, 2010, 化学工学会 研究発表講演要旨集, 2010, 9 - 9, Japanese
- 公益社団法人 化学工学会, 2010, 化学工学会 研究発表講演要旨集, 2010, 774 - 774, Japanese
- Italian Association of Chemical Engineering - AIDIC, 2010, Chemical Engineering Transactions, 20, 103 - 108, English[Refereed]International conference proceedings
- 2010, IBIC2010: 2ND INTERNATIONAL CONFERENCE ON INDUSTRIAL BIOTECHNOLOGY, 20, 103 - 108, English[Refereed]International conference proceedings
- Jan. 2010, ENZYME AND MICROBIAL TECHNOLOGY, 46(1) (1), 51 - 55, English[Refereed]Scientific journal
- Jan. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85(3) (3), 643 - 650, English[Refereed]Scientific journal
- Jan. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85(3) (3), 413 - 423, English[Refereed]Scientific journal
- Dec. 2009, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(24) (24), 7858 - 7861, English[Refereed]Scientific journal
- Dec. 2009, JOURNAL OF BIOCHEMISTRY, 146(6) (6), 867 - 874, English[Refereed]Scientific journal
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(5) (5), 369 - 371, English[Refereed]Scientific journal
- Aug. 2009, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(15) (15), 5175 - 5178, English[Refereed]Scientific journal
- Aug. 2009, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 59(4) (4), 297 - 301, English[Refereed]Scientific journal
- Aug. 2009, BIOTECHNOLOGY LETTERS, 31(8) (8), 1259 - 1264, English[Refereed]Scientific journal
- Jul. 2009, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 19(13) (13), 3619 - 3622, English[Refereed]Scientific journal
- Jun. 2009, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(12) (12), 4149 - 4154, English[Refereed]Scientific journal
- Jun. 2009, JOURNAL OF BIOCHEMISTRY, 145(6) (6), 701 - 708, English[Refereed]Scientific journal
- May 2009, ENZYME AND MICROBIAL TECHNOLOGY, 44(5) (5), 344 - 349, English[Refereed]Scientific journal
- Mar. 2009, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 19(5) (5), 1473 - 1476, English[Refereed]Scientific journal
- Feb. 2009, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 82(1) (1), 59 - 66, English[Refereed]Scientific journal
- 公益社団法人 化学工学会, 2009, 化学工学会 研究発表講演要旨集, 2009, 728 - 728, Japanese
- 公益社団法人 化学工学会, 2009, 化学工学会 研究発表講演要旨集, 2009, 727 - 727, Japanese
- 公益社団法人 化学工学会, 2009, 化学工学会 研究発表講演要旨集, 2009, 601 - 601, Japanese
- 平成14年度に日本政府の総合戦略「バイオマス・ニッポン」が策定され、バイオマスの有効利用による持続的に発展可能な社会の実現が提言されている。この戦略は、(1)バイオマスの有効利用に基づく地球温暖化防止や循環型社会形成の達成、(2)日本独自のバイオマス利用法の開発による戦略的産業の育成を目指すものである。また、地球規模での環境保護の観点から、バイオマス原料は日本のみならず、世界中から安価かつ豊富な資源の積極的な利用が求められている。さらに石油資源枯渇や価格高騰の影響より、これまでの石油資源依存型の「オイルリファイナリー」から、バイオマスベースの「バイオリファイナリー」社会への転換が急務とされている。米国においても、1999年にクリントン大統領が「バイオ製品およびバイオマスエネルギーの開発・普及に関する大統領令」を発令し、バイオマスエネルギーの利用率を2010年までに10%までに拡大する方針を指示し、ヨーロッパにおいても同様の方針が打ち出されている状況である。このような社会的背景も重なり、現在、グルコースから生産されるバイオエタノールはブラジル、米国をはじめとして多くの地域でガソリンと混合され、自動車用燃料として利用されている。しかしながら、食料価格高騰の問題原因ともなっており、代替原料が模索されている。木質系バイオマスはデンプンよりもはるかに豊富に存在し、また古紙や廃材、農産廃棄物など処理が問題化しているものを利用するため原料が安価であることなどから、将来の石油代替エネルギー資源として特に有望視されている。Japanese Society of Grassland Science, 2009, Japanese Journal of Grassland Science, 55(3) (3), 270 - 273, Japanese
- Titanium dioxide (TiO_2) is known as sonocatalyst which generate hydroxyl radicals when the ultrasonic (US) is irradiated to TiO_2 (TiO_2/ US method). In this study, we investigated the cell damage effect and antitumor effect by the combination of ultrasonic irradiation and TiO_2 nanoparticles that is modified with preSl/S2 protein for targeting HepG2 cells. For irradiating of US to the preS1/S2-TiO_2 nanoparticles incorporated HepG2, cell injuring efficiency was evaluated by Trypan blue dye exclusion test. As a result, the cytostatic activity was acquired when irradiated at 1MHz, 0.1 W/ cm^2, 30 second, 50% duty in the presence of TiO_2 nanoparticle modified with preS1/S2 protein. This cytostatic activity was observed at 24 hour after US irradiation, and this effect was significantly enhanced as time elapsed. Also, antitumor effect by TiO_2 / U.S. method in vivo was evaluated. When ultrasonic irradiation was performed by the frequency of 3 times per week, and the irradiation conditions of 1 MHz, 1 W/cm^2, 1 min, no significant damages to the mice was ovserved. These irradiation condition revealed the antitumor effect significantly. Moreover the further research, such as the examination of the irradiation conditions of ultrasound, injection amount of TiO_2 nanoparticles and the specific binding ability to HepG2 tumor after i.v. injection, are proposed.The Japan Society of Sonochemistry, 2009, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 18, 5 - 6, Japanese
- 公益社団法人 化学工学会, 2009, 化学工学会 研究発表講演要旨集, 2009, 912 - 912, Japanese
- 公益社団法人 化学工学会, 2009, 化学工学会 研究発表講演要旨集, 2009, 242 - 242, Japanese
- Dec. 2008, JOURNAL OF BIOCHEMISTRY, 144(6) (6), 701 - 707, English[Refereed]Scientific journal
- Dec. 2008, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 81(4) (4), 711 - 719, English[Refereed]Scientific journal
- Sep. 2008, ULTRASONICS SONOCHEMISTRY, 15(6) (6), 988 - 994, English[Refereed]Scientific journal
- Sep. 2008, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 55(1-2) (1-2), 69 - 75, English[Refereed]Scientific journal
- Sep. 2008, Proceedings of the 10th International Chemical and Biological Engineering Conference-CHEMPOR 2008, Braga, Portugal (on CD-ROM), E20, 4pages, EnglishBioethanol Production from Sugars by Yeast Having Sugar Uptake Ability by Expression of Sugar Transporter[Refereed]Scientific journal
- Jun. 2008, Colloids and Surfaces B: Biointerfaces, Vol 64, pp. 10-15, EnglishProperties of TiO2-Polyacrylic acid (PAA) Dispersions having Molecular Recognition Ability[Refereed]Scientific journal
- May 2008, Journal of Biochemistry, 143(5) (5), 667 - 674, English[Refereed]Scientific journal
- May 2008, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 79(1) (1), 51 - 60, English[Refereed]Scientific journal
- May 2008, JOURNAL OF HAZARDOUS MATERIALS, 153(1-2) (1-2), 551 - 556, English[Refereed]Scientific journal
- 公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 328 - 328, Japanese
- 公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 990 - 990, Japanese
- To well characterize disinfection system and to investigate the mechanisms of the cell killing by TiO_2/U.S. method, Escherichia coli (NOVABLUE strain) was used as a model and target microorganism. Our previous studies reported that ultrasonic irradiation was very effective to kill the microbial cells. Microbial cells are physically and chemically sterilized by ultrasonic irradiation. Our present study was under taken, to investigate a suitable mechanism for cells killing and to develop the sterilization effect using electrochemically under U.S. in the presence of TiO_2. We suggested that, toxic substances such as free OH radical generated by electrolysis have been shown to be responsible for the sterilization effect.The Japan Society of Sonochemistry, 2008, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 17, 16 - 17, Japanese
- Strong bacteriocidal activity of titanium dioxide (TiO_2) irradiated with ultrasound (U.S.) is an established phenomenon. A new non-woven TiO_2 fabric under U.S. system was used to well characterize the sonocatalytic disinfection and lipid peroxidation of Escherichia coli (E. coli) cells membrane and to investigate the possible killing mechanisms of its cell. It was found that applying ultrasonic irradiation with non-woven TiO_2 fabrics reduces the E. coli cell concentration very rapidly, which indicated very high disinfection rate at this system. Diphenyl-1-pyrenylphosphin (DPPP) was used as a non-fluorescent probe for lipid peroxidation on E. coli cells membrane. Reaction of DPPP with U.S. treated samples and organic hydroperoxides were examined by monitoring increase in fluorescence intensity of DPPP oxide (DPPP=O). The mechanism of cell killing and lipid peroxidation were further investigated by examining the effect of OH radical scavenger. The scavenger studies indicated the functional role of hydroxyl (OH) radicals in the initiation of ultrasound induced lipid peroxidation.The Japan Society of Sonochemistry, 2008, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 17, 48 - 49, English
- Effects of TiO_2 particles addition on OH radical formation by ultrasonic irradiation were investigated through comparison of Al_2O_3 particles. Formation of cavitation bubbles is accelerated on the surface of particles because sonoluminescence intensity and white noise intensity are increased by particle addition. It is suggested that there is the close relationship between the effeciency of TiO_2 on OH radical formation and cavitation phenomena. Irradiation frequency is the very important factor for cavitation phenomena. In this reseach, the effects of frequency changes on OH radical formation by ultrasonic irradiation were investigated in the presence of TiO_2.The Japan Society of Sonochemistry, 2008, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 17, 50 - 51, Japanese
- Our previous study demonstrated that the titanium dioxide (TiO_2) could catalyze production of hydroxyl (OH) radicals by ultrasound (U.S.) irradiation even without any photo-excitation. The purpose of this study was to extend the applicability of our "TiO_2/U.S. system" to drug delivery systems for cancer therapy by modifying the surface of TiO_2 nanoparticles with PreS1/S2 protein for targeting HepG2 cells. To evaluate the specific binding of the preS1/S2-conjugated TiO_2 particles (preS1/S2-TiO_2), liver and colon cancer cell lines, HepG2 and WiDr were incubated with the preS1/S2-TiO_2 (0.01% (v/v)) for 6 hours and subjected to fluorescent immunostaining with anti-preS1 antibody. The fluorescence was detected only in the case of HepG2 cells, indicating specific binding and incorporation of preS1/S2-TiO_2. To evaluate the sonocatalytic cytotoxicity of HepG2 cells with incorporation of preS1/S2-TiO_2, the culture dish was irradiated with U.S. at the frequency of 1MHz and with output power of 0.3 W/m^2 for 30 sec (50% duty). Compared to the control condition without preS1/S2-TiO_2 incorporation and/or U.S. irradiation, the growth of HepG2 cells was restrained with both the preS1/S2-TiO_2 incorporation and the U.S. irradiation at initial time of culture, suggesting our "TiO_2/U.S. system" could be promising tool for cancer therapy.The Japan Society of Sonochemistry, 2008, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 17, 96 - 97, Japanese
- 公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 380 - 380, Japanese
- 公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 381 - 381, Japanese
- 公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 348 - 348, Japanese
- 公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 225 - 225, Japanese
- 2008, Appl. Microbiol. Biotechnol., 79: 51-60, EnglishGrowth acceleration of plants and mushroom by erythritol[Refereed]
- Oct. 2007, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 104(4) (4), 339 - 342, English[Refereed]Scientific journal
- Jul. 2007, ENZYME AND MICROBIAL TECHNOLOGY, 41(1-2) (1-2), 156 - 161, English[Refereed]Scientific journal
- Jun. 2007, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1774(6) (6), 671 - 678, English[Refereed]Scientific journal
- Feb. 2007, ULTRASONICS SONOCHEMISTRY, 14(2) (2), 184 - 190, English[Refereed]Scientific journal
- The Japan Society of Sonochemistry, 2007, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 2007, 50 - 51, English
- 公益社団法人 化学工学会, 2007, 化学工学会 研究発表講演要旨集, 2007, 391 - 391, Japanese
- The Japan Society of Sonochemistry, 2007, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 2007, 102 - 102, English
- 公益社団法人 化学工学会, 2007, 化学工学会 研究発表講演要旨集, 2007, 353 - 353, Japanese
- Nov. 2006, BIOCHEMICAL ENGINEERING JOURNAL, 32(2) (2), 100 - 105, English[Refereed]Scientific journal
- Mar. 2006, WATER RESEARCH, 40(6) (6), 1137 - 1142, English[Refereed]Scientific journal
- Titanium dioxide (TiO_2) is known as photocatalytic material, and it generate reactive oxygen species (ROS) according to be excited by ultraviolet rays (UV) under 380nm. Those ROS generated from TiO_2 could sterilize E coli, and have cytotoxic effect on mammalian cancer cells. Previously, we reported the generation of OH radicals from TiO_2 particles excited by ultrasonic (US) irradiation, not UV. Furthermore, the TiO_2 excited by US exhibited decompose activity on E coli and chemical substances. In this study, we report the cytotoxic effect of US in the presence of TiO_2 particles in cell culture media. To estimate the cytotoxic effect, we measured lactase dehydrogenase (LDH) activity leaked from damaged cell numbers after US irradiation. Immediately after US irradiation in the presence of TiO_2 particles, the LDH activity exhibited high, indicating TiO_2 with US irradiation caused damage on cell membrane stability. Furthermore, we confirmed the cell membrane damage by flowcytometry assay with Annexin V-FITC/Propidium iodide (PI) stain method, Annexin V-negative/PI positive cells were dominant immediately after US irradiation in the presence of TiO_2 particles.The Japan Society of Sonochemistry, 2006, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 15, 1 - 2, Japanese
- Generation of OH radicals by ultrasound irradiation in the presence of TiO_2 was studied. The effects of dissolved argon and xenon applied into the irradiating solutions were studied in the presence of TiO_2. Heat capacity ratio of argon and xenon is higher than that of nitrogen and oxygen. Higher heat capacity ratio of dissolved argon and xenon causes higher local temperature when cavitation bubble collapsed, and it results in the induced pyrolysis of H_2O and generation of OH radicals. Further, the effect of the photocatalysis of TiO_2 may be more intensified by high temperature and sonoluminescence.The Japan Society of Sonochemistry, 2006, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 15, 99 - 100, Japanese
- The formation of H_2O_2 was studied with ultrasound irradiation in the presence of TiO_2 or Al_2O_3 particles. The effect of varying the temperature of cavitation bubbles in aqueous solutions of two rare gases (Ar, Xe or mixture of Xe and Ne (Xe:Ne=3:7)) on the formation of H_2O_2 were estimated. The very high temperatures (several thousand K) of collapsing gas bubbles lead to the thermal dissociation of water molecules into OH radicals, and the reaction of two OH radicals causes the formation of H_2O_2 consequently. After 60 min sonication with 25〜36kHz ultrasound the highest concentration of H_2O_2 (612μM) was observed when Xe was saturated in the solution. While the increased concentration of H_2O_2 after 60 min sonication was lower (135μM) in the solution saturated with Ar or mixture of Xe and Ne. The difference in the increased concentration of H_2O_2 was not significant between Ar and mixture of Xe and Ne. These results suggest that the threshold for the formation of cavitation bubbles seems to be the lowest in the solution saturated with Xe and to be the important factor for the formation of H_2O_2 during the sonication in the presence of TiO_2 or Al_2O_3, respectively.The Japan Society of Sonochemistry, 2006, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 15, 87 - 88, Japanese
- To well characterize disinfection system and to investigate the mechanism of cell killing by TiO_2/US method, Escherichia coli (E. coli) was used as a model and target microorganism. At present, our research is going on developing disinfection system by using ultrasonic reactor in the presence of nonwoven TiO_2 fabric. It was found that the decrease in cell concentration was almost insignificant without ultrasonic irradiation although TiO_2 filter was present, and vice versa. In contrast, applying ultrasonic irradiation with TiO_2 filter reduces the E. coli cell concentration very rapidly which indicated very high disinfection power at this condition or system. However, the effect of new ultrasonic reactors on the disinfection rate, the characterization of affecting parameters of the ultrasonic reactors, as well as possible germicidal mechanism of disinfection remain to be studied. Given an opportunity to conduct these research will uncover above issues and will be benefited our scientific career to develop, in addition to enrich the bio-industrial field as well.The Japan Society of Sonochemistry, 2006, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 15, 103 - 104, English
- 公益社団法人 化学工学会, 2006, 化学工学会 研究発表講演要旨集, 2006, 997 - 997, Japanese
- 公益社団法人 化学工学会, 2006, 化学工学会 研究発表講演要旨集, 2006, 783 - 783, Japanese
- Oct. 2005, BIOCHEMICAL ENGINEERING JOURNAL, 25(3) (3), 243 - 248, English[Refereed]Scientific journal
- The Japan Society of Sonochemistry, 2005, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 14, 13 - 14, English
- Generation of OH radicals by ultrasonic irradiation in the presence of TiO_2 was studied. OH radicals are oxidizing agents and effective for the degradation of hazardous organic compounds. However, the mechanisms of OH radicals formation have not been elucidated yet. In this work, the effects of cavitation induced by ultrasonic irradiation in water was considered and investigated. As the lifetime of OH radical is very short and can be hardly analyzed directly, then sodium salicylate was used as OH radicals trapping agent. The addition reaction of OH radicals and salicylate results in the formation of dihydroxy benzoic acid(DHBA). The generation of DHBA by ultrasonic irradiation in the presence of TiO_2 as an indicator of OH radicals was investigated in complete darkness. The effects of particles (TiO_2 and A1_2O_3) on the generation of DHBA were examined(Fig.2). While no generation of DHBA was detected in the solutions without particles, higher DHBA concentration was obtained in the presence of TiO_2 than that of Al_2O_3. This enhancement in the formation of OH radicals may be due to the pyrolysis of H_2O and the photocatalysis of TiO_2 with sonoluminessence. Sonoluminessence may be due to cavitation, and the exsitence of particles couse more cavitation whuch may increase sonoluminessence effect. [graph] Also, the effects of dissolved argon applied into the irradiating solutions were studied in the presence of TiO_2 and Al_2O_2 (Fig.5, 6). Higher amount of DHBA was obtained in the solutions in the presence of TiO_2. Thermal capacity of argon is higher than that of nitrogen and oxygen. Higher thermal capacity of dissolved argon causes higher local temperature during cavitation, and it results in the induced pyrolysis of H_2O and generation of OH radicals. Moreover, the effect of the photocatalysis of TiO_2 may be more intensified in the presence of dissolved argon. [graph] [graph] Consequently, the advantage of TiO_2 in the ultrasonic system for the formation of OH radicals was shown in this research.The Japan Society of Sonochemistry, 2005, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 14, 91 - 93, Japanese
- 公益社団法人 化学工学会, 2005, 化学工学会 研究発表講演要旨集, 2005, 287 - 287, Japanese
- 公益社団法人 化学工学会, 2005, 化学工学会 研究発表講演要旨集, 2005, 280 - 280, Japanese
- 2005, 4th International Symposium on Therapeutic Ultrasound, 754, 275 - 277, EnglishImprovement of ultrasonic disinfection power using TiO2 photocatalyst[Refereed]International conference proceedings
- Jun. 2004, Applied Microbiology and Biotechnology, Vol 64, pp. 823-828, EnglishOver-expression system of phospholipase D from actinomycete by Streptomyces lividans[Refereed]Scientific journal
- Mar. 2004, JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 79(3) (3), 273 - 276, English[Refereed]Scientific journal
- A phospholipase D (PLD) expression system was constructed from Streptoverticillium cinnamoneum using Escherichia coli as a host. From a sequence alignment analysis of PLD superfamily, a conserved sequence, a GG/S motif, was found, next to the putative active center in the PLD superfamily. Based on the hypothesis that a serine residue in GG/S motif is involved in the catalytic function, a serine residue substituted mutant library (sixteen PLD mutants) was constructed. An analysis of the activities of these mutants showed that two serine residue substituted mutants (G215S and G216S) in the GG motif exhibited high conversion activity. However, the substrate specificity of these mutants differed depending on the donor molecules. When a serine amino acid was used as a donor substrate, G216S showed a high transphosphatidylation activity. For other donors, G215S showed a higher catalytic activity than G216S. These results support the hypothesis that the serine residue in the GG/S motif controls substrate specificity and enhances the turnover of catalytic function.The Society of Chemical Engineers, Japan, 2004, Asian Pacific Confederation of Chemical Engineering congress program and abstracts, 2004, 810 - 810, English
- A disinfecting method has been considered by ultrasonic irradiation of water in the presence of TiO2. Titanium dioxide in the rutile-granule form was used as a catalyst in order to generate hydroxyl radicals for cell killing. Escherichia coli and Legionella pneumophila were used for kinetically investigation of cell destruction process. Significant decrease in the concentration of viable cells was observed during irradiation in the presence of TiO2. About 98% reduction in the concentration of viable cells could be obtained in the presence of 1.0 g/ml TiO2 during 30 min of irradiation, while only 13% reduction was observed in the ordinary ultrasonic irradiation system. The rate of cell killing was higher in the presence of TiO2 comparing to Al2O3. The rate of disinfection was proportional to the amount of TiO2 used in the irradiated samples. There was no significant effect of cell concentration on the cell-killing rate in the range of 103 to 107 CFU/ml. Mechanism of cell killing was further investigated by examining the effect of hydroxyl radical (*OH) scavengers. Ascorbic acid, histidine and glutathione were used in the irradiating samples as radical scavengers. The rate of disinfection was decreased in the samples containing these radical scavengers. Also, a continuous irradiating system was devised and applied for high throughput water disinfection process. Contaminated water containing E. coli was disinfected successfully within two hours of irradiation in the continuous system.The Society of Chemical Engineers, Japan, 2004, Asian Pacific Confederation of Chemical Engineering congress program and abstracts, 2004, 610 - 610, English
- Introduction Titanium dioxide in the rutile-pellet form was used as a catalyst in the ultrasonic irradiation system to generate hydroxyl radicals in order to disinfect the water. Cultivated culture of Legionella was used for the kinetically investigation of cell destruction and its mechanism. The formation of active species such as hydroxyl radical, hydrogen peroxide, superoxide and etc can be promoted during ultrasonic irradiation in the presence of TiO_2. It may be due to the concentrated energy of the exploded bubbles on the surfaces of titanium granules caused during acoustic cavitations. These reactive oxygen species (ROS) may disrupt or damage various cell or viral functions and structures. Improvement of this novel disinfection method and studying the mechanism of this system were the purposes of this investigation. Materials and methods -Preparation of Cultures Legionella pneumophila(GIFU 9888) was used in this research as the model microorganisms. Legionella were incubated on BCYE plate and transferred to BYE medium. The inoculated culture was incubated in a shaker incubator at 110 rpm for 48 hrs at 37℃. Then, the culture broth was diluted to a suitable concentration. Ten ml of the diluted broth was transferred to the bottles containing TiO_2 pellets at different concentrations. -Ultrasonic irradiation Bottles were irradiated at 36 KHz, 300 W and samples were driven at different time courses. The temperature was kept constant at 20℃ during irradiation. The irradiated samples were diluted to suitable concentrations and plated on BCYE plates. The plates were incubated for 72 hrs at 37℃. The number of colonies on the plates was counted after incubation. Results and Discussion Ultrasonic irradiation of diluted culture for 15 minutes showed 92% reduction in the number of viable cell concentration at the presence of TiO_2. The rate of disinfection was compared in the presence of TiO_2 and Al_2O_3. TiO_2 induced higher rate of cell killing compared with Al_2O_3. Also, investigating the effect of TiO_2 concentration indicated that the rate of disinfection was proportional to the concentration of TiO_2. The mechanism of cell killing was further investigated by examining the effects of OH radical scavengers such as ascorbic acid and glutathione. Lower rates of disinfection were observed in the samples containing these radical scavengers, thus indicating the primary role of OH radicals in this process.The Japan Society of Sonochemistry, 2004, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 13, 38 - 40, Japanese
- Irradiation of TiO_2 particles under ultraviolet light has been known to be a source of OH radicals in the disinfection or oxidizing systems. However, a new system was devised in this study for production of OH radicals by replacing UV with ultrasonic system (US). Formation of OH radicals was investigated in a TiO_2/US system to study the mechanism of formation of OH radicals as well as enhancement of cavitation effect in the ultrasonic system. Formation of OH radicals in the irradiating solution was studied by the reaction of OH radicals with salicylate. 2,3-dihydroxybenzoicacid (2,3-DHBA) and 2,5-dihydroxybenzoicacid (2,5-DHBA) can be formed during this reaction and they can be measured by HPLC with an electro chemical detector (HPLC-ECD; Eicom). Formation of DHBAs could be detected during the irradiation of salicylate solution under ultrasonic system and in the presence of TiO_2. It was seen that the concentration of DHBAs was increased within 20 minutes irradiation and then decreased in spite of high concentration of salicylate. It was likely due to the oxidation and degradation of DHBA in the ultrasonic system. Therefore, for investigating the oxidation of DHBA in the presence of salicylate, more salicylate was added after 20 minutes reaction to the irradiating mixture consecutively. The concentration of DHBA was increased for three times by the addition of salicylate concentration in the solution. Enhancement of cavitation energy was also investigated by the addition of various gases (Ar, O2, N2) into irradiation solution. The ratio of the specific heats at constant pressure and constant volume (Cp/Cv) of the gas and vapor inside the cavity is known to be one of the most crucial parameters that determine the final temperature inside cavitation bubbles. Monoatomic gases, such as argon has a high value of Cp/Cv, while diatomic gases, such as N_2, O_2 have a lower values. Therefore, addition of argon to the irradiating solution induced higher concentration of OH radicals due to higher cavitation energy. Our results indicated that the addition of O_2 and N_2 gases at the same conditions resulted lower oxidation power. Finally, the competitive oxidation of salicylate and DHBAs in the irradiating solutions was more investigated in this research to develop a more descriptive mechanism of generation of hydroxyl radicals.The Japan Society of Sonochemistry, 2004, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 13, 94 - 96, Japanese
- 2004, Applied and Microbiogy and Biotechnology, 64,823-828, EnglishOver-expression system for secretory phospholipase D by Streptomyces livedanse[Refereed]Scientific journal
- Dec. 2003, BIOCHEMICAL ENGINEERING JOURNAL, 16(3) (3), 273 - 278, English[Refereed]Scientific journal
- Sep. 2003, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 23(2-6) (2-6), 107 - 115, English[Refereed]Scientific journal
- Sep. 2003, BIOCHEMICAL ENGINEERING JOURNAL, 15(3) (3), 193 - 197, English[Refereed]Scientific journal
- Aug. 2003, APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 110(2) (2), 101 - 112, EnglishImprovement in enzyme activity and stability by addition of low molecular weight polyethylene glycol to sodium bis(2-ethyl-L-hexyl)sulfosuccinate/isooctane reverse micellar system[Refereed]Scientific journal
- The Japan Society of Sonochemistry, 2003, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 12, 30 - 32, Japanese
- Jan. 2003, KAGAKU KOGAKU RONBUNSHU, 29(1) (1), 124 - 130, JapaneseStructural investigation of water trapped in AOT/isooctane reverse micelles containing PEG by Fourier transform infrared spectroscopy[Refereed]Scientific journal
- Mar. 2002, BIOCHEMICAL ENGINEERING JOURNAL, 10(2) (2), 115 - 121, EnglishImprovement of transphosphatidylation reaction model of phospholipase D from Streptoverticillium cinnamoneum[Refereed]Scientific journal
- Heterogeneous photocatalysis over TiO_2 or ultrasonic irradiation have been reported to be a promising application for decomposition of the hazardous chemical substances. We observed the generation of ・OH in the aqueous solution during the irradiation of ultrasound on TiO_2. The purpose of this study was to verify the generation of ・OH by indicating (1) decomposition of methylene blue, (2) nucleophilic addition of ・OH to salicylic acid, which produce dihydroxybenzoic acid. The decomposition of bisphenol A (BPA) and pentachlorophenol (PCP) by the irradiation of ultrasound in presence of TiO_2 were further studied. The results are summarized as follows. Decomposition of methylene blue Experimentally it was observed that although the Al_2O_3 had no effect on the decomposition of methylene blue by ultrasonic irradiation, it was decomposed slightly by the presence of TiO_2. In addition H_2O_2 accelerated the decomposition of methylene blue for the TiO_2 containing system. But the addition of H_2O_2 had no effect on the methylene blue decomposition when the system containing Al_2O_3 only. The effect of radical scavengers on the decomposition of methylene blue was also investigated for the system with TiO_2. It was found that the scavengers such as methanol, DMSO, mannitol reduced the decomposition. Among them DMSO was the most significant. Effect of TiO_2 on the nucleophilic addition of ・OH to salicylic acid Ultrasonification of 10 μM salicylic acid (2 ml) was carried out in absence and presence of 0.4 g TiO_2 for exactly 10 minutes, and produced dihydroxybenzoic acid (DHBA) was measured by HPLC/ECD. In presence of TiO_2, significant amount of DHBA (34 pg/20μl) was produced, but almost no DHBA (2.2 pg/20μl) was found to produce without TiO_2. The effect of scavengers on the generation of DHBA was the same as it was observed in the case of methylene blue decomposition. This result inferred that the ultrasonic irradiation generated the ・OH, which is responsible for the hydroxylation of salicylic acid. Decomposition of BPA and PCP Ultrasonification of BPA and PCP, a representative endocrine disruptors, were carried out in the presence of TiO_2 (0.4 g) and the change in the concentration of BPA and PCP were measured by HPLC/ECD. The initial concentration of BPA (1 ppm) was gradually decreased with the irradiation time and was totally decomposed by 15 minute irradiation of ultrasound. The similar results were observed for PCP. It was concluded that the irradiation of ultrasound on TiO_2 in aqueous solution significantly generate hydroxyl radicals which is responsible for the decomposition of the endocrine disruptors such as BPA and PCP.The Japan Society of Sonochemistry, 2002, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 11, 91 - 93, Japanese
- 2002, Environmental Toxicology, 17(5) (5), 441 - 448, English[Refereed]Scientific journal
- Jan. 2001, BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1530(1) (1), 23 - 31, EnglishIdentification of novel membrane-bound phospholipase D from Streptoverticillium cinnamoneum, possessing only hydrolytic activity[Refereed]Scientific journal
- Dec. 2000, BRAIN RESEARCH, 887(1) (1), 178 - 182, EnglishThe 5-HT1A receptor agonist, 8-OH-DPAT, attenuates stress-induced anorexia in conjunction with the suppression of hypothalamic serotonin release in rats[Refereed]Scientific journal
- Feb. 1999, JOURNAL OF BIOCHEMISTRY, 125(2) (2), 263 - 269, EnglishPurification, characterization, and sequence determination of phospholipase D secreted by Streptoverticillium cinnamoneum[Refereed]Scientific journal
- Elsevier B.V., Jun. 1996, FEBS Letters, 387(2-3) (2-3), 141 - 144, English[Refereed]Scientific journal
- Apr. 1996, Proceedings of the National Academy of Sciences of the United States of America, 93(9) (9), 4300 - 4304, English[Refereed]Scientific journal
- Dec. 1995, Proceedings of the National Academy of Sciences of the United States of America, 92(26) (26), 12319 - 12322, English[Refereed]Scientific journal
- Oct. 2022, PROCESSES, 10(10) (10), 2004 - 2004, English[Refereed]
- Apr. 2022, 化学工学 [特集]「生物機能を利用したモノづくり」に貢献するプロセス強化, 86(4) (4), 151 - 152, Japaneseバイオエコノミーの展望Introduction scientific journal
- 2022, 日本生物工学会大会講演要旨集, 74thComparative analysis of metabolic strategies among various yeast species
- 2021, 化学工学会年会研究発表講演要旨集(CD-ROM), 86thパーム油産業廃棄物POMEを原料とする微生物による酵素・化学品への変換
- 2021, 日本生物工学会大会講演要旨集, 73rdBreeding of organic acid assimilation yeast for bio-refinery
- 2021, 日本生物工学会大会講演要旨集, 73rdPolyhydroxyalkanoic acid production by an oleaginous yeast strain Lipomyces starkeyi
- 2021, 日本生物工学会大会講演要旨集, 73rd13C-metabolic fluxes analysis of Yarrowia lipolytica in growth and stationary phases
- 2021, 質量分析総合討論会講演要旨集, 69thApplication of 13C-Metabolic flux analysis to the oleaginous yeast Yarrowia lipolytica
- 2020, 日本農芸化学会中部支部例会講演要旨集(Web), 187th好冷性海洋細菌が産生するβ-1,3-キシラナーゼの機能解析
- 2019, 日本生物工学会大会講演要旨集, 71st油脂酵母Lipomyces starkeyiの遺伝子的改変による長鎖長脂肪酸の生産
- 2019, 日本生物工学会大会講演要旨集, 71st好冷性海洋細菌Psychroflexus torquis由来β-1,3-キシラナーゼの酵素化学的諸性質
- 07 Aug. 2018, 日本生物工学会大会講演要旨集, 70th, 191, Japanese機械学習を用いた酵素のEC number予測
- 日本DDS学会, May 2018, 日本DDS学会学術集会プログラム予稿集, 34回, 204 - 204, Japanese過酸化チタンナノ粒子の併用による放射線増感治療法の開発
- 05 Mar. 2018, 日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 68th, ROMBUNNO.Z14‐02‐0930, Japaneseバイオマスのイオン液体前処理と酵素糖化により得られる残渣リグニンのアルカリ酸化分解特性
- 2017, 日本作物学会講演会要旨集, 243rdデンプン合成制御因子CRCTが稲わらを用いたバイオエタノール生産に及ぼす効果
- 14 Oct. 2016, リグニン討論会講演集, 61st, 180‐181, Japanese疎水性アルコールを用いた植物バイオマスの効率的な成分分離法の開発
- 25 Aug. 2016, 日本生物工学会大会講演要旨集, 68th, 316, Japanese凝集性酵母における新規な阻害剤耐性機構の解明
- 13 Mar. 2016, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N316, EnglishYeast breeding for fuels and chemicals production from Indonesia Culture Collection
- 13 Mar. 2016, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N321, EnglishEconomic Value of Current Main Use of Oil Palm Empty Fruit Bunches (EFB) and Its Potential Use for Biofuel
- 13 Mar. 2016, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N302, EnglishBiorefinery research in Kobe and Indonesia
- 13 Mar. 2016, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N313, EnglishBiorefinery as strategic approach for supporting of utilization of tropical lignocellulosic biomas
- 05 Mar. 2016, 日本農芸化学会大会講演要旨集(Web), 2016, 4A022 (WEB ONLY), Japaneseマンナンバイオマスからのエタノール生産:β‐マンナナーゼとβ‐マンノシダーゼを細胞表層に提示した出芽酵母の開発
- 05 Mar. 2016, 日本農芸化学会大会講演要旨集(Web), 2016, 3A030 (WEB ONLY), Japaneseアルコールの添加が希硫酸前処理の効率に与える影響の解析
- 2016, 日本農芸化学会大会講演要旨集(Web), 2016紙パルプの同時糖化発酵によるカフェ酸の微生物生産
- Aug. 2015, CANCER RESEARCH, 75, EnglishSummary international conference
- 09 Mar. 2015, 日本植物生理学会年会要旨集, 56th, 284, Japanese2次元NMRを用いた希硫酸前処理後固体画分の組成解析
- 05 Mar. 2015, 日本農芸化学会大会講演要旨集(Web), 2015, 3B33A01 (WEB ONLY), Japanese実バイオマスを微生物変換するための新しい酵母プラットフォームの探索
- 2015, 育種学研究, 17バイオリファイナリー利用に向けた稲わらの多様性
- 日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 95 - 95, Japanese1P-025 Improvement of astaxanthin production using metabolically engineered Xanthophyllomyces dendrorhous
- 日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 320 - 320, Japanese3P-198 Characterics studies of Streptoverticillium cinnamoneum phospholipase D promoter for gene expression
- 日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 218 - 218, Japanese2P-173 Ionic liquid pretreatment and in situ enzymatic saccharification of bagasse using biocompatible cholinium ionic liquid
- 日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 217 - 217, Japanese2P-170 Fran monomer production from lignocellulosic biomass pretreated and fractionated using acidic ionic liquid
- 日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 217 - 217, Japanese2P-171 High solid saccharification and subsequent fermentation from the biomass pretreated with minimum amount of ionic liquid
- 日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 217 - 217, Japanese2P-172 Lignomonomer production from residue obtained by ionic liquid pretreatment and enzymatic saccharification of lignocellulosic biomass
- 日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 199 - 199, Japanese2P-097 Metabolic engineering of L-Lactate producing Aspergillus oryzae.
- (公社)日本医学放射線学会, Feb. 2014, Japanese Journal of Radiology, 32(Suppl.) (Suppl.), 37 - 37, Japanese過酸化チタンナノ粒子による放射線増感の検討
- 日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 206 - 206, Japanese3P-045 Effects of hydrolysate of lignocellulosic biomass on phenyllactate fermentation by recombinant Escherichia coli
- 日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 251 - 251, Japanese3P-226 A display of pH-sensitive fusogenic GALA peptide facilitates endosomal escape for a Bio-nanocapsule
- 日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 72 - 72, Japanese1P-219 Membrane process increased ethanol production from pretreated rice straw.
- 日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 28 - 28, Japanese1P-044 Simultaneous saccharification and fermentation of D-lactic acid from starch by Aspergillus oryzae
- 日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 45 - 45, Japanese1P-112 Production of p-aminobenzoic acid by Streptomyces lividans expressing pabAB
- 日本生物工学会, 2014, 生物工学会誌 : seibutsu-kogaku kaishi, 92(2) (2), 71 - 71, JapaneseDirect isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display system
- (一社)日本消化器外科学会, Jul. 2013, 日本消化器外科学会総会, 68回, O - 82, Japanese超音波触媒法による胆管ステント再閉塞予防の検討
- 小峰工業出版, Feb. 2013, 化學工業, 64(2) (2), 136 - 139, JapaneseSimultaneous Saccharification and Fermentation of Ethanol from Ionic Liquid Treated Biomass
- 日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 47 - 47, Japanese1P-118 Metabolic engineering for homogentisic acid production in Streptomyces lividans
- 日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 44 - 44, Japanese1P-105 Simultaneous saccharification and fermentation of cellulose for phenyllactic acid production
- 日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 47 - 47, Japanese1P-117 Production of protocatechuate by Corynebacterium glutamicum
- 日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 63 - 63, Japanese1P-183 Simultaneous saccharification and fermentation from lignocellulosic material pretreated by biocompatible ionic liquid
- 日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 72 - 72, Japanese1P-218 Pretreatment of lignocellulosic biomass by acid-catalysed process in aqueous ionic liquid solution
- 日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 141 - 141, Japanese2P-149 Proposal of a cost-effective bioethanol production process using commercial ionic liquids
- 日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 141 - 141, Japanese2P-148 Sugar concentration in liquid hydrolysate of dilute acid pretreated rice straw by nanofiltration
- 日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 231 - 231, Japanese3P-174 Development of affibody-displaying bio-nanocapsule for specific delivery to EGFR-expressing cells
- 05 Nov. 2012, 化学工学 = Chemical engineering, 76(11) (11), 722 - 722, Japanese神戸大学大学院工学研究科応用化学専攻 生物化学工学研究室
- Nov. 2012, INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 84(3) (3), S700 - S700, EnglishNovel Radiosensitization Through Reactive Oxygen Species Generation Using Titanium Peroxide Nanoparticle Compounds Against Pancreas CancerSummary international conference
- Sep. 2012, NEW BIOTECHNOLOGY, 29, S50 - S50, EnglishSummary international conference
- (一社)日本消化器外科学会, Jul. 2012, 日本消化器外科学会総会, 67回, 1 - 1, Japaneseナイチノールステント・超音波触媒法による再閉塞予防の検討
- (一社)日本外科学会, Mar. 2012, 日本外科学会雑誌, 113(臨増2) (臨増2), 126 - 126, Japanese外科領域における先端技術・治療の開発 酸化チタン・超音波触媒法によるラジカル生成を利用した肝胆膵悪性腫瘍に対する医工融合型治療法の開発
- (一社)日本外科学会, Mar. 2012, 日本外科学会雑誌, 113(臨増2) (臨増2), 389 - 389, Japanese
- 日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 248 - 248, Japanese4Ip22 Saccharification and fermentation of biomass by the ionic liquid pretreatment with the engineered xylose fermentable yeast
- 日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 239 - 239, Japanese4Ia02 Ethanol production from woody biomass by ionic liquid pretreatment
- 日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 230 - 230, Japanese4Ha05 Construction of biomass-degradation enzymes library from Streptomyces and exploring novel high-level secretion signal
- 日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 186 - 186, Japanese4Ba09 Characterization of DNA aptamer selected by Cell SELEX for human hepatic cancer cells
- 日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 208 - 208, Japanese4Dp13 Benzoic acid fermentation from starch and cellulose using the polyketide synthesis pathway of Streptomyces maritimus
- 日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 205 - 205, Japanese4Dp02 Production of cadaverine by Corynebacterium glutamicum
- 2012, 酵素工学研究会講演会講演要旨集, 68thイオン液体耐性セルラーゼの単離と酵素特性解析
- 2012, 日本生物工学会大会講演要旨集, 64thイオン液体耐性セルラーゼの探索と機能解析
- 2012, 日本農芸化学会大会講演要旨集(Web), 2012海洋性超好熱菌Thermotoga neapolitana由来β-1,3-キシラナーゼの耐熱性を導く要因の解析
- 2012, 日本農芸化学会大会講演要旨集(Web), 2012海洋性超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの構造安定性
- 2012, 日本農芸化学会大会講演要旨集(Web), 2012麹菌Aspergillus oryzaeを用いたラクダ科動物由来一本鎖抗体可変部位VHH分泌生産系の構築
- (一社)日本癌治療学会, Sep. 2011, 日本癌治療学会誌, 46(2) (2), 1046 - 1046, Japanese超音波と酸化チタンを用いた音響力学療法の基礎的検討
- 05 Mar. 2011, 日本農芸化学会大会講演要旨集, 2011, 47, JapaneseStreptomyces griseocarneusスフィンゴミエリナーゼCの速度論的解析とMg2+による活性増強メカニズムの解明
- 日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 69 - 69, Japanese1Kp11 IPA production from cellobiose by E. coli displayed BGL
- 日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 71 - 71, Japanese1Lp01 Application of bio-molecule modified nano-particle in biochemical engineering
- 日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 24 - 24, Japanese1Ap14 Development of ionic liquid-based consolidated bioprocessing (i-CBP)
- 日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 28 - 28, Japanese1Bp17 Direct Glutathione Fermentation from Biomass by Engineered Yeast
- 日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 23 - 23, Japanese1Ap13 Ethanol production from ionic liquid pretreated biomass with cellulase expressing yeast
- 日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 23 - 23, Japanese1Ap12 Effect of ionic liquid pretreatment of crystalline cellulose on enzymatic saccharification efficiency
- 日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 179 - 179, Japanese2Ip12 Efficient glutathione production by novel recombinant yeast containing high intracellular glutathione
- 日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 42 - 42, Japanese1Ep12 Production of thermostable β-1,4-mannanase from hyperthermophilic eubacterium Thermotoga neapolitana by Aspergillus oryxae
- 日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 201 - 201, Japanese2Lp13 Characterization of DNA aptamer selected by Cell-SELEX for human hepatic cancer cells
- 2011, 日本農芸化学会関西支部講演会講演要旨集, 472nd麹菌Aspergillus oryzaeを用いたラクダ由来一本鎖抗体可変部位VHH分泌生産系の構築
- 2011, 日本生物工学会大会講演要旨集, 63rd麹菌Aspergillus oryzaeによる超好熱菌Thermotoga neapolitana由来耐熱性β-1,4-マンナナーゼの生産
- 2011, 日本生物工学会大会講演要旨集, 63rdAffibody提示Bio-nanocapsuleを用いたHER2発現癌細胞へのタンパク質送達システム
- 2011, 日本生物工学会大会講演要旨集, 63rd麹菌Aspergillus oryzaeを用いたラクダ由来一本鎖抗体可変部位VHHの生産
- 日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 180 - 180, Japanese2Ip13 Affibody displaying Bio-nanocapsule for protein therapy to HER2 expressing cancer cells
- フレグランスジャーナル社, Oct. 2010, Fragrance journal, 38(10) (10), 39 - 44, JapaneseInnovative BioProduction Kobe (iBioK) Project in Kobe University
- 25 Sep. 2010, 日本生物工学会大会講演要旨集, 62nd, 30, JapaneseStreptomyces griseocarneus NBRC13471由来スフィンゴミエリナーゼCの大量発現
- 公益社団法人日本生物工学会, 25 Sep. 2010, 日本生物工学会大会講演要旨集, 22, 30 - 30, Japanese2P-1085 Overexpression of shingomyelinase C from Streptomyces griseocarneus NBRC13471
- 公益社団法人日本生物工学会, 25 Sep. 2010, 日本生物工学会大会講演要旨集, 22, 30 - 30, Japanese2P-1086 Overexpression and kinetics of sphingomyelinase C from Streptomyces griseocarneus NBRC13471
- 日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 35 - 35, Japanese2P-1106 Over-production of various secretory-form proteins in Streptomyces lividans
- 日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 197 - 197, Japanese2B-Ba05 Single-cell analysis and agonist screening for G-protein-coupled receptors
- 日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 168 - 168, Japanese3P-2082 Development of non-invasive cancer cell killing method based on combination of titanium peroxiside nano-particle and low intensity X-ray irradiation
- 日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 154 - 154, Japanese3P-2043 Development of ionic liquid-based consolidated bioprocessing (i-CBP), part. 3.
- 日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 169 - 169, Japanese3P-2084 Selection of DNA aptamer for human hepatic cancer cells by Cell SELEX
- 日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 102 - 102, Japanese3P-1180 Affibody displaying Bionanocapsules for specific drug delivery to HER2 expressing cancer cell
- 日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 118 - 118, Japanese2P-2047 Direct ethanol production from cellulose by using cellulolytic enzymes expression optimized yeast strain
- 日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 233 - 233, Japanese3S-Da03 Construction of biomaterial conjugated nano-particles for environmental and medical application.
- 2010, 日本生物工学会大会講演要旨集, 62nd超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの機能解析
- 2010, 日本生物工学会大会講演要旨集, 62nd麹菌による超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの高分泌生産
- 2010, Journal of Applied Glycoscience, 57(Suppl.) (Suppl.)麹菌(Aspergillus oryzae)を宿主とした超好熱菌Thermotoga neapollitana由来耐熱性β-1,3-キシラナーゼの分泌高生産
- 2010, Journal of Applied Glycoscience, 57(Suppl.) (Suppl.)超好熱菌Thermotoga neapollitana由来耐熱性β-1,3-キシラナーゼの発現および諸性質
- The Society for Biotechnology, Japan, Nov. 2009, Journal of bioscience and bioengineering, 108(1) (1), S27, EnglishBP-P10 Affibody displaying bionanocapsules for HER2 specific drug delivery(Section II Biopharmaceuticals Production)
- The Society for Biotechnology, Japan, Nov. 2009, Journal of bioscience and bioengineering, 108(1) (1), S64, EnglishBE-O21 New SELEX strategy for screening of DNA aptamer by AFM(Section V Biomolecular Engineering and Bioseparation)
- The Society for Biotechnology, Japan, Nov. 2009, Journal of bioscience and bioengineering, 108(1) (1), S71, EnglishBE-P21 Screening of DNA aptamers that recognize amino acid by AFM-SELEX strategy(Section V Biomolecular Engineering and Bioseparation)
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S64 - S64, EnglishSummary international conference
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S50 - S50, EnglishSummary international conference
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S27 - S27, EnglishSummary international conference
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S50 - S50, EnglishSummary international conference
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S47 - S48, EnglishSummary international conference
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S159 - S160, EnglishSummary international conference
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S48 - S48, EnglishSummary international conference
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S51 - S52, EnglishSummary international conference
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S51 - S51, EnglishSummary international conference
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S49 - S49, EnglishSummary international conference
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S48 - S49, EnglishSummary international conference
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S52 - S52, EnglishSummary international conference
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S36 - S37, EnglishSummary international conference
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S71 - S71, EnglishSummary international conference
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S43 - S43, English[Refereed]Summary international conference
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S53 - S53, English[Refereed]Summary international conference
- Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S53 - S53, English[Refereed]Summary international conference
- ティー・アイ・シィー, Jun. 2009, Materials integration, 22(6) (6), 8 - 11, JapaneseCancer therapy based on sonocatalytic radical generator from photocatalytic nano-particle
- 日本生物工学会, 2009, 日本生物工学会大会講演要旨集, 21, 57 - 57, Japanese1Ep22 Development of Affibody displaying Bio-nanocapsule for targeted cancer therapy
- 日本生物工学会, 2009, 日本生物工学会大会講演要旨集, 21, 170 - 170, Japanese2La01 Efficient ethanol production from raw starch using novel yeast strain
- 日本生物工学会, 2009, 日本生物工学会大会講演要旨集, 21, 172 - 172, Japanese2La11 Development of novel cell-surface display system using porin in Corynebacterium glutamicum
- 日本生物工学会, 2009, 日本生物工学会大会講演要旨集, 21, 173 - 173, Japanese2Lp02 Efficient protein production in recombinant Aspergillus oryzae
- 化学工学会, 05 Nov. 2008, 化学工学 = Chemical engineering, 72(11) (11), 636 - 638, Japanese二酸化チタン/超音波触媒法とその医療応用
- 05 Nov. 2008, 石油・石油化学討論会講演要旨, 38th, 211, JapaneseStreptomyces griseocameus NBRC13471由来スフィンゴミエリナーゼC遺伝子のクローニングと発現
- 30 Oct. 2008, バイオイメージング, 17(2) (2), 110 - 111, Japanese特異性改変型バイオナノカプセルを用いたバイオイメージング及びDDSへの応用
- Aug. 2008, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 236, EnglishORGN 297-Phospholipid conversion in ionic liquid by phospholipase DSummary international conference
- 金沢大学環日本海域環境研究センター = Institute of Nature and Environmental Technology, Kanazawa University, Japan, 31 Jul. 2008, 年報 金沢大学環日本海域環境研究センター = K-INET Kanazawa University, 2007, 129 - 134, JapanesePinpoint delivery of TiO2 nano-particles to the cancer cells in vitro
- 金沢大学環日本海域環境研究センター = Institute of Nature and Environmental Technology, Kanazawa University, Japan, 31 Jul. 2008, 年報 金沢大学環日本海域環境研究センター = K-INET Kanazawa University, 2007, 135 - 140, JapaneseSonocatalytic formation of H2O2 following sonication of water in the presence of TiO2
- Jun. 2008, COLLOIDS AND SURFACES B-BIOINTERFACES, 64(1) (1), 10 - 15, English
- 化学工業社, Feb. 2008, ケミカルエンジニヤリング, 53(2) (2), 140 - 144, Japanese微生物を用いたバイオ・リファイナリー戦略--バイオマスから燃料・化学品の生産 (特集 バイオマス利用技術の展望)
- 日本生物工学会, 2008, 日本生物工学会大会講演要旨集, 20, 184 - 184, Japanese2Gp20 Construction of the cellulase expression system in Streptomyces lividans
- 日本生物工学会, 2008, 日本生物工学会大会講演要旨集, 20, 156 - 156, Japanese1Fp15 Gene cloning and expression of sphingomyelinase C from Streptomyces griseocarneus NBRC13471
- 日本生物工学会, 2008, 日本生物工学会大会講演要旨集, 20, 158 - 158, Japanese2Fa01 Phospholipid Conversion in Ionic Liquid by Phospholipase D
- 日本生物工学会, 2008, 日本生物工学会大会講演要旨集, 20, 60 - 60, Japanese2Aa08 Efficient ethanol production from raw starch by delta-integrant polyploid fusants
- 2008, 酵素工学研究会講演会講演要旨集, 60th, 71, JapaneseStreptomyces griseocarneus NBRC13471由来スフィンゴミエリナーゼCの遺伝子クローニングと発現
- Gene cloning and expression of sphingomyelinase C from Streptomyces griseocarneus NBRC13471<i>S. griseocarneus</i> NBRC13471スフィンゴミエリナーゼC遺伝子<i>smc</i>をクローニングした。次に、発現ベクターpUC702に<i>smc</i>を導入し、<i>Streptoverticillium cinnamoneum</i>を宿主として発現を行った。その結果、形質転換体の培養上清液中に約100 mg/mlの本酵素を分泌生産させることができた。しかしながら、培養上清中の酵素比活性は3 mU/mgと元株の約100分の1であった。公益社団法人 石油学会, 2008, Proceeding of Annual/Fall Meetings of the Japan Petroleum Institute, 2008(0) (0), 142 - 142, Japanese
- 日本工業出版, Sep. 2007, 超音波techno, 19(5) (5), 41 - 45, Japanese二酸化チタン・超音波触媒法による細胞死誘導 (特集 ソノケミストリーの最新動向(2))
- Sep. 2007, JOURNAL OF BIOTECHNOLOGY, 131(2) (2), S185 - S186, EnglishSummary international conference
- Sep. 2007, JOURNAL OF BIOTECHNOLOGY, 131(2) (2), S237 - S238, EnglishSummary international conference
- 日本工業出版, Jul. 2007, 超音波techno, 19(4) (4), 45 - 49, Japanese二酸化チタン・超音波触媒法を応用した有害微生物殺菌システム (特集 ソノケミストリーの最新動向(1))
- 日本工業出版, Jul. 2007, 超音波techno, 19(4) (4), 39 - 44, Japanese二酸化チタン・超音波触媒法によるOHラジカルの生成と溶存希ガスの促進効果 (特集 ソノケミストリーの最新動向(1))
- 日本空気清浄協会, 2007, Journal of Japan Air Cleaning Association, 45(1) (1), 24 - 31, JapaneseFormation of OH radicals during ultrasonic irradiation with Ti02 and its application to the elimination of hazardous chemicals and bacteria
- 日本生物工学会, 2007, 日本生物工学会大会講演要旨集, 19, 213 - 213, Japanese2J11-1 Effect of signal peptide sequence on secretory protein production in Streptomyces lividans
- 日本生物工学会, 2007, 日本生物工学会大会講演要旨集, 19, 82 - 82, Japanese2C10-3 Mutation analysis of phospholipid methabolite enzyme by Streptmyces as a host strain
- 日本生物工学会, 2006, 日本生物工学会大会講演要旨集, 18, 142 - 142, Japanese2H12-5 Interaction analysis of DNA aptamer that recognizes 8-hydroxy-2-deoxyguanosine
- 日本生物工学会, 2006, 日本生物工学会大会講演要旨集, 18, 202 - 202, Japanese2K12-4 Intracellular uptake of TiO_2 nanoparticles and sonodynamic injury of tumor cell membrane
- 日本生物工学会, 2006, 日本生物工学会大会講演要旨集, 18, 203 - 203, Japanese2K12-5 Synthesis and modification of fusogenic liposome encapsulated with TiO_2 nanoparticles
- 日本生物工学会, 2006, 日本生物工学会大会講演要旨集, 18, 212 - 212, Japanese1L10-5 Investigation of recombinant production of phospholipase D by Streptomyces
- ティ-・アイ・シ-, Mar. 2005, マテリアルインテグレ-ション, 18(3) (3), 31 - 36, Japanese二酸化チタンへの超音波照射によって発生するラジカルの応用 (特集 いろいろつかえるソノケミストリー(1))
- 化学工業社, Feb. 2005, 化学工業, 56(2) (2), 106 - 111, Japanese二酸化チタン・超音波照射法による大腸菌殺菌メカニズム (特集/ソノケミストリーの新しい進展)
- 日本生物工学会, 2005, 日本生物工学会大会講演要旨集, 17, 153 - 153, Japanese2E11-2 Effect of lid structure of phospholipase D on transphosphatidylation activity
- 日本生物工学会, 2005, 日本生物工学会大会講演要旨集, 17, 152 - 152, Japanese2E10-3 Construction of recombinant protein expression system by using the promoter region from Streptomyces cinnamoneum
- バイオインダストリー協会, 01 Dec. 2004, Bioscience & industry, 62(12) (12), 809 - 812, JapaneseProduction of phospholipase D and its application for phospholipid synthesis
- 日本生物工学会, 2004, 日本生物工学会大会講演要旨集, 16, 146 - 146, Japanese3C11-4 Screening and characterization of nucleotide recognized DNA aptamer
- 日本生物工学会, 2003, 日本生物工学会大会講演要旨集, 15, 71 - 71, JapaneseCharacterization of mutants phospholipase D from Streptoverticillium cinnamoneum
- 日本生物工学会, 2002, 日本生物工学会大会講演要旨集, 14, 171 - 171, Japanese927 Biosensing of benzene derivatives with Ps promoter gene and reporter protein GFP
- 日本生物工学会, 2002, 日本生物工学会大会講演要旨集, 14, 112 - 112, Japanese672 Catalytic function analysis of phospholipase D by SiteDirect Mutagenesis
- 日本生物工学会, 2000, 日本生物工学会大会講演要旨集, 12, 183 - 183, JapaneseConstruction and Investigation of Mutant Phospholipase D from Streptoverticillium cinnamoneum
- 日本生物工学会, 1999, 日本生物工学会大会講演要旨集, 11, 92 - 92, JapaneseExpression and characterization of fused PLDα from Stv. cinnamoneum in E. coli.
- 日本生物工学会, 1998, 日本生物工学会大会講演要旨集, 10, 166 - 166, JapanesePurification and characterization of a novel Phospholipase D from Streptoverticillium cinnamoneum
- 日本生物工学会, 1997, 日本生物工学会大会講演要旨集, 9, 134 - 134, JapanesePurification and cloning of Phospholpiase-D from Streptoverticllium cinnamoneum
- 日本生物工学会, 1996, 日本生物工学会大会講演要旨集, 8, 189 - 189, JapaneseStudy on PLD of actinomycete for signal transduction
- Joint work, Springer Nature, May 2019, EnglishYeast Cell Surface Engineering : "Energy Production: Biodiesel"Scholarly book
- Joint work, Elsevier, Mar. 2018, EnglishA cancer treatment strategy that combines the use of inorganic/biocomplex nanoparticles with conventional radiation therapy, Nanoparticle Technology HandbookScholarly book
- Joint work, Springer, Apr. 2015, EnglishGreen Processes for NanotechnologyScholarly book
- Joint work, テックタイムス, Aug. 2014, Japanese紙パルプ産業と環境 2015Scholarly book
- Joint work, 丸善出版, Jan. 2014, Japanese化学便覧 応用化学編Scholarly book
- Joint work, コロナ社, Sep. 2013, Japanese基礎から学ぶ生物化学工学演習Scholarly book
- Joint work, シーエムシー出版, May 2013, Japaneseリサイクルバイオテクノロジーの最前線, 第I編 第1章, 1 未来型CBP法によるバイオエタノール生産技術の展開Scholarly book
- Joint work, シーエムシー出版, Dec. 2012, Japanese次世代医薬開発に向けた抗体工学の最前線 (第Ⅱ編 抗体の改変技術,第4章 親和性の向上,第4節 「酵母による抗体フラグメントおよび抗体様結合性タンパク質の改変技術」)Scholarly book
- Joint work, シーエムシー出版, Dec. 2010, JapaneseエコバイオリファイナリーScholarly book
- Joint work, シーエムシー出版, Apr. 2010, Japaneseグリーンバイオケミストリーの最前線Scholarly book
- Joint work, シーエムシー出版, Mar. 2010, Japaneseシングルセル解析の最前線 (第3章 細胞内生体分子群の実測定量解析,第4節 「フローサイトメトリーとGFPレポーターによるG蛋白質シグナルのシングルセル解析」)Scholarly book
- Joint work, シーエムシー出版, Apr. 2009, Japanese第二世代バイオ燃料の開発と応用展開Scholarly book
- Single work, シーエムシー出版, Jun. 2008, Japanese微生物によるものづくりScholarly book
- Single work, 学進出版, Feb. 2008, Japanese二酸化チタンと超音波で殺菌?!Scholarly book
- Joint work, 関西化学機械製作株式会社創立60周年記念出版, Feb. 2008, Japanese進化・増殖する化学工学Scholarly book
- Joint work, ケミカルエンジニアリング, 2008, Japanese微生物を用いたバイオ・リファイナリー戦略:バイオマスからの燃料・化学品の生産General book
- Joint work, 超音波テクノ, Sep. 2007, Japanese二酸化チタン・超音波触媒法による細胞死誘導Scholarly book
- Joint work, 超音波テクノ, Jul. 2007, Japanese二酸化チタン・超音波触媒法を応用した有害微生物殺菌システムScholarly book
- Joint work, 超音波テクノ, Jul. 2007, Japanese二酸化チタン・超音波触媒法によるOHラジカルの生成と溶存希ガスの促進効果Scholarly book
- Joint work, 空気清浄, May 2007, Japanese二酸化チタン・超音波触媒法の環境浄化、殺菌への応用Scholarly book
- Joint work, 最新医学, Jun. 2006, Japaneseナノテクノロジーによる新規がん治療法.Scholarly book
- Single work, 生物工学会誌, Jan. 2006, Japanese二酸化チタンと超音波で殺菌?Scholarly book
- Joint work, NTS, Apr. 2005, Japanese超音波利用技術集成「超音波と二酸化チタンを用いた有害微生物の殺菌」Scholarly book
- Joint work, マテリアルインテグレーション, Mar. 2005, Japanese二酸化チタンへの超音波照射によって発生するラジカルの応用Scholarly book
- Joint work, 化学工業, Feb. 2005, Japanese二酸化チタン・超音波照射による大腸菌殺菌メカニズムScholarly book
- Joint work, バイオサイエンスとインダストリー, Dec. 2004, JapaneseホスホリパーゼDの生産とリン脂質合成への適用Scholarly book
- Joint work, Recent Research Developments in Physiology, 2003, EnglishStress-induced serotonin release in the hypothalamus and suppression of food intake in ratsScholarly book
- Joint work, 三恵社, Mar. 2002, Japaneseテッシュエンジニアリングの展望Scholarly book
- Single work, INSEF, 2002, EnglishFunctional analysis of phospholipase D and its application for phospholipids synthesisScholarly book
- Single work, 生物工学会誌, Jul. 2000, JapaneseエコエネルギーScholarly book
- 日本農芸化学会2020年度大会メタボローム解析が明らかにする低酸素濃度におけるコリネ型細菌の芳香族化合物代謝促進機構と代謝改変によるソルガム搾汁液からの物質生産
- 第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference油脂酵母Lipomyces starkeyi の遺伝子的改変による長鎖長脂肪酸の生産Oral presentation
- 第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference日本酒生産にヒントを得た麹菌酵素カクテルと酵母を用いた高純度希少糖生産Oral presentation
- 第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference新奇な好熱性放線菌Streptomyces thermoviolaceus を用いたタンパク質生産株の開発Oral presentation
- 第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference酵母カルチャーコレクションからのバイオエタノール生産のための優良酵母の選抜と評価Oral presentation
- 第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference好冷性海洋細菌Psychroflexus torquis 由来β-1,3-キシラナーゼの酵素化学的諸性質Oral presentation
- 第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference好熱性放線菌Streptomyces thermoviolaceus による物質生産Oral presentation
- 第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference基質酵素反応予測のための 機械学習に基づく予測モデルの構築方法の探索と評価Oral presentation
- 第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference過酸化チタンナノ粒子 (PAA -TiOx )の 細胞内傷害Oral presentation
- 第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conferenceイオン液体で前処理した木質系バイオマス中の多糖およびリグニンのリファイナリーOral presentation
- 第21回化学工学会学生発表会, Mar. 2019, Japanese, 京都大学桂キャンパス, Domestic conference新奇な好熱性放線菌Streptomyces thermoviolaceusを用いたタンパク質生産株の開発Poster presentation
- 第21回化学工学会学生発表会, Mar. 2019, Japanese, 京都大学桂キャンパス, Domestic conference完全分散型麹菌のプロテオーム解析Poster presentation
- 日本農芸化学会2019年度大会, Mar. 2019, Japanese, 東京農業大学 世田谷キャンパス, Domestic conferenceピルビン酸代謝フロー改変を用いた 2,3-ブタンジオール 高生産麹菌の構築Poster presentation
- 日本農芸化学会2019年度大会, Mar. 2019, Japanese, 東京農業大学 世田谷キャンパス, Domestic conferenceナノポアシーケンサを用いた遺伝子組換え麹菌のゲノミ クスおよびトランスクリプトミクスPoster presentation
- 第21回化学工学会学生発表会, Mar. 2019, Japanese, 京都大学桂キャンパス, Domestic conferencePEG化チタンナノ粒子の種々の表面修飾による特性解析Poster presentation
- 18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), 2019, APCChE, Sapporo Convention CenterBio-refinery strategy by collaboration Asian's partnership
- 18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), 2019, APCChE, Sapporo Convention CenterDirect increase of intracellular hydrogen peroxide and enhancement for radiosensitivity with polyacrylic acid-modified titanium peroxide nanoparticles
- 第18回糸状菌分子生物学コンファレンス, Nov. 2018, Japanese, シティホールプラザ「アオーレ長岡」, Domestic conference乳酸生産麹菌のピルビン酸ミトコンドリアキャリアタンパク質欠損における代謝変動Poster presentation
- 第13回ムギ類研究会, Nov. 2018, Japanese, 横浜市立大学木原生物学研究所, Domestic conference外来遺伝子発現用のコムギ細胞外排出シグナルと乾燥誘導性プロモーターの効果Poster presentation
- 第18回糸状菌分子生物学コンファレンス, Nov. 2018, Japanese, シティホールプラザ「アオーレ長岡」, Domestic conference黄麹菌における汎用性高発現プロモーターPoster presentation
- 第18回糸状菌分子生物学コンファレンス, Nov. 2018, Japanese, シティホールプラザ「アオーレ長岡」, Domestic conferenceマルチコピー遺伝子導入黄麹菌のゲノム構造解析Poster presentation
- 異業種交差点Ⅱ, Nov. 2018, Japanese, 新化学技術推進協会, 一橋講堂 2階中会議室, Domestic conferenceバイオマスから基礎化学品原料をつくる(2)Poster presentation
- The 24th Symposium of Young Asian Biological Engineers’ Community (YABEC 2018), Nov. 2018, English, National Taiwan University, International conferenceTarget cancer therapy by inorganic nanoparticleOral presentation
- The 24th Symposium of Young Asian Biological Engineers’ Community (YABEC 2018), Nov. 2018, English, National Taiwan University, International conferenceCaffeic acid production from lignocellulosic biomass by metabolically engineered Eshrichaia coliOral presentation
- 第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference油脂酵母Lipomyces starkeyi D35株の 繰り返し発酵による脂肪酸組成変化解析Poster presentation
- 第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference免疫クリアランスを回避する生体膜由来ドラッグデリバリーキャリアの開発Poster presentation
- 第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference乳酸高生産性黄麹菌における乳酸生産フラックス強化による乳酸生産性の向上Poster presentation
- 第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会, 関西大学 千里山キャンパス, Domestic conference二次代謝系の動的特性解析における大過剰化合物測定の有無の影響の数理解析Poster presentation
- 第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference組換えコリネ型細菌を用いた3-アミノ-4-ヒドロキシ安息香酸の発酵生産における酸素制限による 促進作用Poster presentation
- 第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference最少量の低毒性イオン液体を用いた前処理に基づく木質系バイオマスリファイナリーPoster presentation
- 第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference最少量の低毒性イオン液体を用いたバイオマスからバイオ燃料へのHigh-loadingワンポット変換Poster presentation
- 第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference麹菌内で同調的発現挙動を示すプロモーターを用いたタンパク質生産挙動の解析Poster presentation
- 第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference好熱性放線菌Streptomyces thermoviolaceus の培養特性解析Poster presentation
- 第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference原子間力顕微鏡を用いたB型肝炎感染機構の解明Poster presentation
- 第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference機械学習を用いた酵素のEC number予測Poster presentation
- 第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conferenceバイオマス分解酵素の大規模スクリーニングで見出した希少放線菌Poster presentation
- 第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conferenceナノポアシーケンサを用いた遺伝子組換え麹菌ゲノム上の長鎖タンデムリピート構造の解析Poster presentation
- 第70回日本生物工学会大会, Sep. 2018, English, 関西大学 千里山キャンパス, Domestic conferenceThe enhancement of culture conditions for high cell and lipid production of oleaginous yeast Lipomyces starkeyii D35Poster presentation
- 第70回日本生物工学会大会, Sep. 2018, English, 関西大学 千里山キャンパス, Domestic conferenceSelection of oleaginous yeast capable of high lipid accumulating during challenges from inhibitory chemical complexesPoster presentation
- 第70回日本生物工学会大会, Sep. 2018, English, 関西大学 千里山キャンパス, Domestic conferenceRepeated ethanol fermentation from membrane-concentrated sweet sorghum juice by flocculating yeast Saccharomyces cerevisiaePoster presentation
- 第70回日本生物工学会大会, Sep. 2018, English, 関西大学 千里山キャンパス, Domestic conferencePreparation and characterisation of robust whole cell biocatalyst from Aspergillus oryzae expressing Fusarium heterosporum lipase immobilized onto activated carbon for biodiesel synthesisPoster presentation
- 第70回日本生物工学会大会, Sep. 2018, English, 関西大学 千里山キャンパス, Domestic conferenceOptimization on biodiesel synthesis from palm oil mill effluent (POME) by integrated strategy with lipasecatalyzed ethanolysisPoster presentation
- 第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conferencemRNAサイレンシングを用いた過酸化チタンナノ粒子の放射線増感機序解明Poster presentation
- 第70回日本生物工学会大会, Sep. 2018, English, 関西大学 千里山キャンパス, Domestic conferenceImprovement of mass mixing by Max-Blend and anchor shape impeller in the high-solid state scarification of kraft pulpPoster presentation
- 第70回日本生物工学会大会, Sep. 2018, English, 関西大学 千里山キャンパス, Domestic conferenceEffect of immobilized Chlorella Sorokiniana culture on lipid accumulation and CO2 sequestrationPoster presentation
- 第70回日本生物工学会大会, Sep. 2018, English, 関西大学 千里山キャンパス, Domestic conferenceDilute maleic acid pretreatment of sugarcane bagasse and oil palm empty fruit bunch fiber for enhanced enzymatic digestibilityPoster presentation
- 第34回日本DDS学会学術集会, Jun. 2018, Japanese, 長崎ブリックホール, Domestic conference免疫クリアランスの回避を目指した生体膜由来キャリアの開発Poster presentation
- 第34回日本DDS学会学術集会, Jun. 2018, Japanese, 長崎ブリックホール, Domestic conference新規放射線増感剤としての過酸化チタンナノ粒子の物性解析Poster presentation
- 第34回日本DDS学会学術集会, Jun. 2018, Japanese, 長崎ブリックホール, Domestic conference過酸化チタンナノ粒子の併用による放射線増感治療法の開発Poster presentation
- 第7回JACI/GSCシンポジウム, Jun. 2018, Japanese, 新化学技術推進協会, ANA CROWNE PLAZA KOBE, Domestic conferenceバイオマスから基礎化学品原料をつくるPoster presentation
- The 8th International Conference on Algal Biomass, Biofuels and Bioproducts (AlgalBBB 2018), Jun. 2018, English, Motif Seattle, International conferenceConverting Chlamydomonas sp. JSC4 lipids to biodiesel via biocatalysis with Fusarium heterosporum lipase-expressing Aspergillus oryzae whole-cellPoster presentation
- 化学工学会,第83年会, Mar. 2018, Japanese, 関西大学, Domestic conference多孔体粒子を用いた固定化培養槽の撹拌性能調査Poster presentation
- 日本農芸化学会2018年度大会, Mar. 2018, Japanese, 日本農芸化学会, 名古屋市, Domestic conference酸素をスイッチとしたコリネ型細菌中央代謝経路の切り替えPoster presentation
- 日本農芸化学会2018年度大会, Mar. 2018, Japanese, 日本農芸化学会, 名古屋市, Domestic conference麹菌ミトコンドリア; ピルビン酸キャリアタンパク質欠損株におけるピルビン酸代謝フラックス改変による有用化合物生産Poster presentation
- 第3回デザイン生命工学研究会, Mar. 2018, Japanese, デザイン生命工学研究, 沖縄県国頭郡今帰仁村, Domestic conference酵素改変を指向したin silico解析Poster presentation
- 日本農芸化学会2018年度大会, Mar. 2018, Japanese, 日本農芸化学会, 名古屋市, Domestic conferenceリグノセルロース系バイオマスを包括的に利用したコリネ型細菌によるバイオモノマー生産Poster presentation
- 日本化学会第98春季年会2018, Mar. 2018, Japanese, 日本化学会, 船橋市, Domestic conferenceリグノセルロースナノファイバー表面のリグニン分布解析手法の開発Poster presentation
- 日本農芸化学会2018年度大会, Mar. 2018, Japanese, 日本農芸化学会, 名古屋市, Domestic conferenceSaccharomyces cerevisiae酵母におけるCandida boidinii由来キシロース発酵代謝系の導入効果Poster presentation
- 第13回バイオマス科学会議, Jan. 2018, English, 日本エネルギー学会, 仙台市, International conference同時糖化発酵プロセスを利用した紙パルプを原料とするカフェ酸の生産Oral presentation
- 5th Asian Conference on Biomass Science, Jan. 2018, English, 日本エネルギー学会, 仙台市, International conferenceEfficient Xylitol Production from Kraft Pulp by a Cell Surface Engineered Strain ofSaccharomyces cerevisiaePoster presentation
- 第12回ムギ類研究会, Dec. 2017, Japanese, 京都大学, Domestic conference麦わらのバイオマス利用に関するパンコムギ系統間差異Poster presentation
- 第17回糸状菌分子生物学コンファレンス, Nov. 2017, Japanese, 糸状菌分子生物学研究会, 佐賀市, Domestic conference麹菌のミトコンドリア局在ピルビン酸キャリアタンパク質欠損による乳酸生産性の向上Oral presentation
- 第17回糸状菌分子生物学コンファレンス, Nov. 2017, Japanese, 糸状菌分子生物学研究会, 佐賀市, Domestic conference麹菌の co-transformation 時のコピー数多型性に関する解析Oral presentation
- 第7回CSJ化学フェスタ2017, Oct. 2017, Japanese, 日本化学会, 東京都, Domestic conferenceリグニン残量を制御したセルロースから得られた各種ナノファイバーの構造と物性Poster presentation
- The 23th Symposium of Young Asian Biochemical Engineers' Communyty (YABEC2017), Oct. 2017, English, Xi'an, China, International conferenceIn situ detection of an arming yeast cellulase using AFM[Invited]Invited oral presentation
- 第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference発酵阻害物質耐性Candida boidinii K212のキシロース発酵の解析Poster presentation
- 第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference発酵阻害耐性酵母由来強力なENO1プロモーターの発現解析Poster presentation
- 第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference酸素をスイッチとした二つの樹脂原料の個別生産Poster presentation
- 第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference麹菌による複数遺伝子の同時高発現を可能にするプロモーターセットの開発Poster presentation
- 第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference高度膜分離技術によるソルガム搾汁液からのスクロース分離および連続的エタノール生産Oral presentation
- 第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conferenceThe establishment high cell density culture of oleaginous Lipomyces starkeyi D35 for high cell and lipid productionPoster presentation
- 第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conferenceHigh Production of single cell oil from glucose and xylose using oleaginous yeast Lipomyces starkeyiPoster presentation
- 第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conferenceExploration of potential indigenous marine actinomycetes from indonesia soil producing enzyme lignocellulosic for biorefinery applicationPoster presentation
- 第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conferenceEnhanced cell surface engineering of Saccharomyces cerevisiae and optimization of the fermentation process for efficient xylitol production from kraft pulpOral presentation
- 第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conferenceB型肝炎ウイルス由来ペプチドを用いたAFMによる肝細胞の受容体検出Poster presentation
- 第63回高分子研究発表会(神戸), Jul. 2017, Japanese, 高分子学会関西支部, 神戸市, Domestic conferenceリグニン量を制御したセルロースナノファイバーの構造と物性Oral presentation
- 平成29年度繊維学会年次大会, Jun. 2017, Japanese, 繊維学会, 東京都, Domestic conferenceリグニン含有量を異にするセルロースから得られた各種ナノファイバーの構造と物性Poster presentation
- 日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都市, Domestic conference組換え大腸菌によるグルコースを原料とする4-アミノ 桂皮酸の直接発酵生産Oral presentation
- 第19回化学工学会学生発表会, Mar. 2017, Japanese, 化学工学会関西支部, 豊中市, Domestic conference肝細胞認識ペプチドを用いた AFMによる肝癌細胞の力学的評価Oral presentation
- 日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都市, Domestic conference遺伝子組み換え麹菌培養上清カクテルを用いた希少糖高生産プロセスの開発Oral presentation
- 第19回化学工学会学生発表会, Mar. 2017, Japanese, 化学工学会関西支部, 豊中市, Domestic conferenceバイオマスの効率的な分解を志向したマルチセルラーゼ高発現麹菌の開発Oral presentation
- 第2回デザイン生命工学研究会大会, Mar. 2017, Japanese, デザイン生命工学研究会, 神戸市, Domestic conferenceデザインされた生体分子の触診デバイスOral presentation
- 日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都市, Domestic conferenceコリネ型細菌を用いたフェルラ酸からプロトカテク酸の生産Oral presentation
- 第19回化学工学会学生発表会, Mar. 2017, Japanese, 化学工学会関西支部, 豊中市, Domestic conferenceREDOXバランスにおける発酵阻害耐性酵母由来新規TDHxの影響Oral presentation
- 日本作物学会講演会, 2017, Japanese, Domestic conferenceデンプン合成制御因子CRCTが稲わらを用いたバイオエタノール生産に及ぼす効果Poster presentation
- 第16回糸状菌分子生物学コンファレンス, Nov. 2016, Japanese, 糸状菌分子生物学研究会, 宇治市, Domestic conference代謝改変およびフラックス強化した黄麹菌でのデンプンからの乳酸生産Oral presentation
- 第16回糸状菌分子生物学カンファレンス, Nov. 2016, Japanese, 宇治おうばくプラザ, Domestic conference麹菌におけるゲノムを切らずに書き換える新規ゲノム編集ツールの確立Poster presentation
- 第16回糸状菌分子生物学コンファレンス, Nov. 2016, Japanese, 糸状菌分子生物学研究会, 宇治市, Domestic conference麹菌におけるゲノムを切らずに書き換える新規ゲノム編集ツールの確立Oral presentation
- 第16回糸状菌分子生物学コンファレンス, Nov. 2016, Japanese, 糸状菌分子生物学研究会, 宇治市, Domestic conference黄麹菌 Aspergillus oryzae による機能性オリゴ糖の生産Oral presentation
- 第16回糸状菌分子生物学コンファレンス, Nov. 2016, Japanese, 糸状菌分子生物学研究会, 宇治市, Domestic conferenceマルチセルラーゼ発現黄麹菌の培養条件によるセルラーゼ生産性の違いOral presentation
- 第61回リグニン討論会, Oct. 2016, Japanese, リグニン討論会実行委員会, 宇治市, Domestic conference疎水性アルコールを用いた植物バイオマスの効率的な成分分離法の開発Oral presentation
- The 22th Symposium of Young Asian Biochemical Engineers' Communyty, Oct. 2016, English, 生物工学会, 宮崎市, International conferenceMapping of endoglucanases displayed on a surface of yeast cell catalyst using atomic force microscopyOral presentation
- 第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference転写因子改変放線菌を用いた異種タンパク質生産Oral presentation
- 第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference担癌マウスを用いたポリアクリル酸修飾過酸化チタンナノ粒子の生体内分布の解明Oral presentation
- 化学工学会第48回秋季大会, Sep. 2016, Japanese, 化学工学会, 徳島市, Domestic conference新規放射線増感治療に向けた過酸化チタンナノ粒子のマウス生体内分布特性の調査Oral presentation
- セラミック協会第 29 回秋季シンポジウム, Sep. 2016, Japanese, セラミック協会, 東広島市, Domestic conference酸化チタン類ナノ粒子と物理的外部刺激を併用した新規がん治療の提案Oral presentation
- 第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference三種セルラーゼ共・強発現麹菌によるセルロースからのコウジ酸生産Oral presentation
- 第34回日本植物細胞分子生物学会(上田)大会, Sep. 2016, Japanese, 日本植物細胞分子生物学会, 長野県上田市, Domestic conference原料となる植物バイオマスの種類がセルロー スナノファイバーの強度に与える影響の解析Oral presentation
- 第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference凝集性酵母における新規な阻害剤耐性機構の解明Oral presentation
- 第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference遺伝子組換え大腸菌を用いたフェニル乳酸生産に影響を与える遺伝子の発現解析Oral presentation
- 第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conferenceσ因子欠損放線菌の3,4-AHBA生産への影響Oral presentation
- 第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conferenceバイオナノカプセルとリポソームの複合粒子によるin vivoでの抗腫瘍効果Oral presentation
- 化学工学会第48回秋季大会, Sep. 2016, Japanese, 化学工学会, 徳島市, Domestic conferenceナノ粒子とX線照射の併用による低侵襲がん治療法の開発Oral presentation
- 第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conferenceコリスミ酸ピルビン酸リアーゼ発現コリネ型細菌を用いたプロトカテク酸の生産Oral presentation
- 化学工学会第48回秋季大会, Sep. 2016, Japanese, 化学工学会, 徳島市, Domestic conferenceアルコールの添加が希硫酸前処理の効率に与える影響の解析Oral presentation
- 生物工学若手研究者の集い夏のセミナー2016, Jul. 2016, Japanese, 生物工学会, 府中市, Domestic conference膵臓がん治療に向けた新規放射線増感剤としての過酸化チタンナノ粒子のin vitro評価Poster presentation
- 生物工学若手研究者の集い夏のセミナー2016, Jul. 2016, Japanese, 生物工学会, 府中市, Domestic conference担癌マウスを用いたポリアクリル酸修飾過酸化チタンナノ粒子の生体内分布の解明Poster presentation
- 生物工学若手研究者の集い夏のセミナー2016, Jul. 2016, Japanese, 生物工学会, 府中市, Domestic conferenceHER2受容体発現細胞を標的としたDDSキャリアの抗腫瘍効果の評価Poster presentation
- 生物工学若手研究者の集い夏のセミナー2016, Jul. 2016, Japanese, 生物工学会, 府中市, Domestic conferenceAFMで診る・識る・釣る ~核酸・タンパク・細胞の研究~Poster presentation
- 第5回 JACI/GSCシンポジウム, Jun. 2016, Japanese, 公益社団法人 新化学技術推進協会, 神戸市, Domestic conferenceMechanical Properties of Lignocellulose Nanofiber Sheets Prerared in Butanol / Sulfuric Acid MethodPoster presentation
- 第5回JACI GSCシンポジウム, Jun. 2016, Japanese, 新化学技術推進協会, 神戸市, Domestic conferenceアルコールの添加が希硫酸前処理の効率に与える影響の解析Poster presentation
- Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conferenceOptimization of Displayed Cellulases in Yeast Suitable for Ionic Liquid Pretreated Biomass by Cocktail δ Integration MethodologyOral presentation
- Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conferenceEngineering and Analyzing Streptomyces strain for the Recombinant Protein ProductionOral presentation
- Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conferenceDirect Fermentation of L-Lactic Acid from Starch By Genetically Engineered Aspergillus OryzaeOral presentation
- Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conferenceDevelopment of Platform Yeast Strain Capable of Direct Fermentation of Raw Biomass to EthanolPoster presentation
- Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conferenceCaffeic Acid Production By Simultaneous Saccharification and Fermentation of Kraft Pulp Using Recombinant Escherichia coliOral presentation
- 化学工学会第81年会, Mar. 2016, Japanese, Domestic conference担癌マウスを用いたポリアクリル酸修飾過酸化チタンナノ粒子の生体内分布特性の調査Oral presentation
- 日本農芸化学会2016年度大会, Mar. 2016, Japanese, Domestic conference紙パルプの同時糖化発酵によるカフェ酸の微生物生産Oral presentation
- 日本農芸化学会2016年度大会, Mar. 2016, Japanese, Domestic conferenceマンナンバイオマスからのエタノール生産:βーマンナナーゼとβーマンノシダーゼを細胞表層に提示した出芽酵母の開発Oral presentation
- 日本農芸化学会2016年度大会, Mar. 2016, Japanese, Domestic conferenceコリネ型細菌によるUV吸収性アミノ酸の生産Oral presentation
- 日本農芸化学会2016年度大会, Mar. 2016, Japanese, Domestic conferenceアルコールの添加物が希硫酸前処理の効率に与える影響の解析Oral presentation
- The 6th iBioK Asian Workshop, Mar. 2016, Japanese, Kobe, International conferenceFrom mannan and lignocellulosic biomass to biochemicals: cell surface display and metabolic engineering in yeast Saccharomyces cerevisiaeOral presentation
- 化学工学会第81年会, Mar. 2016, Japanese, Domestic conferenceAFM力計測を用いた細胞表層提示酵素の特異的検出とマッピング評価Oral presentation
- 日本化学会第96春季年回, Mar. 2016, Japanese, Domestic conferenceAFM力学計測法を利用した細胞表層空間に提示した酵素検出Oral presentation
- 化学工学会第81年会, Mar. 2016, Japanese, Domestic conferenceAffibody提示バイオナノカプセル/リポソーム融合粒子を用いたin vivo での抗腫瘍効果Oral presentation
- 第11回バイオマス科学会議, Jan. 2016, Japanese, International conferenceリグノセルロース系バイオマスの同時糖化発酵によるフェニル乳酸生産Oral presentation
- 神戸大学若手フロンティア2015, Dec. 2015, Japanese, 神戸大学, Domestic conference稲わらのバイオリファイナリーに向けた特性の解析と多様性の評価Poster presentation
- 日本農芸化学会関西支部第492例会, Dec. 2015, Japanese, 神戸大学, Domestic conferenceバイオリファイナリー利用に向けた稲わらの希硫酸前処理後グルコース含量の自然変異Oral presentation
- The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015), Dec. 2015, English, International conferenceSpecific drug delivery for target cancer tumor using affibody-displaying bionanocapsule/liposome complexOral presentation
- The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015), Dec. 2015, English, International conferenceLive cell imaging and membrane protein mapping with atomic force microscopeOral presentation
- Pacifichem 2015, Dec. 2015, English, International conferenceDevelopment of novel radiosensitizing cancer therapy:Combination of radio-therapy and titanium peroxide nanoparticleOral presentation
- Pacifichem 2015, Dec. 2015, English, International conferenceCharacterization for metal-oxide nano-perticles generating radicals with X-ray irrdiatioOral presentation
- 第15回糸状菌分子生物学コンファレンス, Nov. 2015, Japanese, Domestic conferenceコピー数多形とプロモーター強度を加味した黄麹菌でのマルチ遺伝子発現Oral presentation
- 第15回糸状菌分子生物学コンファレンス, Nov. 2015, Japanese, Domestic conferenceL-乳酸生産黄麹菌の乳酸生産能に対する中央代謝経路改変の影響Oral presentation
- 第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, Japanese, Domestic conference代謝改変による赤色酵母を用いたアスタキサンチン生産性の向上Oral presentation
- 第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, Japanese, Domestic conferenceStreptoverticillium Cinnamoneum由来Phospholipase D プロモーター遺伝子発現機構の調査Oral presentation
- 第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, Japanese, Domestic conferenceL-乳酸生産株Aspergillus oryzaeの代謝改変によるLー乳酸生産能の改変Oral presentation
- The 21th Symposium of Young Asian Biochemical Engineer's Communyty, Oct. 2015, English, International conferenceLignocellulosic biomass refinery using biocompatible ionic liquidaOral presentation
- The 21th Symposium of Yong Asian Biochemical engineer's Communyty, Oct. 2015, English, International conferenceConversion of high phospholipid-containing oils to biodiesel using immobilizes Aspergillus oryzae whole cell biocatalyst ezpressing Fusarium heteroporum lipaseOral presentation
- 化学工学会第47回秋季大会, Sep. 2015, Japanese, Domestic conference放射線治療と相乗効果を有するポリアクリル酸修飾過酸化チタンナノ粒子の作用機序解明Oral presentation
- SCEJ 47th Autumn Meeting, Sep. 2015, Japanese, The Society of Chemical Engineers, Japan, Hokkaido University, Domestic conferencekinetic analysis of the lignin degradation under ultrasonic irradiationOral presentation
- 化学工学会第47回秋季大会, Sep. 2015, Japanese, Domestic conference担癌マウスを用いたポリアクリル酸修飾過酸化チタンナノ粒子の生体内分布特性の調査Oral presentation
- マテリアルサイエンス研究科セミナー, Jul. 2015, Japanese, Domestic conferenceバイオマスからの微生物によるバイオモノマー生産[Invited]Invited oral presentation
- 6th AFOB Regional Symposium, May 2015, English, Asian Federation of Biotechnology, International conferenceScreening of ethanol and lactic acid-producing yeast from Indonesia culture collectionOral presentation
- 6th AFOB Regional Symposium, May 2015, English, Asian Federation of Biotechnology, International conferenceScreening and characterization of Lignocellulose-hydrolytic from Indonesia Rare Actinomycetes and applications of these enzymes for sugar cane baggase saccharification to produce bioethanolOral presentation
- 日本農芸化学会2015年度大会, Mar. 2015, Japanese, 日本農芸化学会, 岡山市, Domestic conference膜濃縮したソルガム搾汁液からのエタノール発酵Oral presentation
- 第17回化学工学会学生発表会, Mar. 2015, Japanese, 化学工学会, 徳島市, Domestic conference担癌マウスへのTiナノ粒子投与による生体内分布Oral presentation
- 日本農芸化学会2015年度大会, Mar. 2015, Japanese, 日本農芸化学会, 岡山市, Domestic conference組換えコリネ型細菌による3-hydroxy-4-aminobenzoic acid発酵におけるソルガム搾汁液の促進作用Oral presentation
- 日本農芸化学会2015年度大会, Mar. 2015, Japanese, 日本農芸化学会, 岡山市, Domestic conference実バイオマスを微生物変換するための新しい酵母プラットフォームの探索Oral presentation
- 日本農芸化学会2015年度大会, Mar. 2015, Japanese, 日本農芸化学会, 岡山市, Domestic conferenceコリネ型細菌を用いたプロトカテク酸の生産Oral presentation
- 日本化学会第95春季年会, Mar. 2015, Japanese, 日本化学会, 船橋市, Domestic conferenceAFM による細胞表層提示タンパク質の特異的検出およびマッピングOral presentation
- Active Enzyme Molecule 2014, Dec. 2014, English, 酵素工学会, 富山市, Domestic conferenceConstruction of arming yeast displaying optimal ratio of cellulases for degradation of biomass pretreated with ionic liquidPoster presentation
- 第14回糸状菌分子生物学コンファレンス, Nov. 2014, Japanese, 糸状菌分子生物学研究会, 仙台市, Domestic conference麹菌細胞工場を用いた L- および D-乳酸の生産Poster presentation
- 第15回極限環境生物学会年会, Nov. 2014, Japanese, 極限環境生物学会, 沖縄市, Domestic conference好熱性および好冷性酵素のドメインを持つキメラ酵素の構築Poster presentation
- 第14回糸状菌分子生物学コンファレンス, Nov. 2014, Japanese, 糸状菌分子生物学研究会, 仙台市, Domestic conferenceセルラーゼ遺伝子をマルチコピー導入した麹菌の遺伝子コピー数とセルラーゼ活性Poster presentation
- The 20th Symposium of Young Asian Biochemical Engineers' Communyty, Nov. 2014, English, 生物工学会, Taiwan, China, International conferenceMicrobial screening for biorefinery researchOral presentation
- 第5回イオン液体討論会, Oct. 2014, Japanese, イオン液体研究会, 横浜市, Domestic conferenceイオン液体前処理後バガスに適したアーミング酵母の開発Oral presentation
- 第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference放線菌を用いた糖を原料とするパラアミノ安息香酸の生産Poster presentation
- 第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference麹菌を用いたデンプンからのD- 乳酸の同時糖化発酵Poster presentation
- 第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference稲わら前処理液の膜プロセスによるエタノール発酵高効率化Poster presentation
- 第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conferenceバイオマス糖化液由来成分が大腸菌のフェニル乳酸発酵に与える影響Poster presentation
- 第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conferencepH 応答性ペプチドGALA を表層提示したバイオナノカプセルのエンドソーム脱出Poster presentation
- 生物工学若手研究者の集い夏のセミナー, Jul. 2014, Japanese, 生物工学会, 神戸市, Domestic conferencepH応答性膜融合ペプチドGALAを表層提示したバイオナノカプセルのエンドソーム脱出Poster presentation
- 生物工学若手研究者の集い夏のセミナー, Jul. 2014, Japanese, 生物工学会, 神戸市, Domestic conference放射線がん治療との併用に向けたか酸化チタンナノ粒子の物性評価Poster presentation
- 生物工学若手研究者の集い夏のセミナー, Jul. 2014, Japanese, 生物工学会, 神戸市, Domestic conference上皮成長因子受容体を特異的に認識するAffibody提示バイオナノカプセルの開発Poster presentation
- 生物工学若手研究者の集い夏のセミナー, Jul. 2014, Japanese, 生物工学会, 神戸市, Domestic conferenceリグノセルロース系バイオマスからのバイオ芳香族化合物の生産Poster presentation
- 第60回高分子研究発表会(神戸), Jul. 2014, Japanese, 高分子学会, 神戸市, Domestic conferenceカーボンナノチューブの表面改質を目指した重合性界面活性剤の開発Poster presentation
- 生物工学若手研究者の集い夏のセミナー, Jul. 2014, Japanese, 生物工学会, 神戸市, Domestic conferenceAFMを用いた表層提示酵母へのアプローチ及び表層提示酵素の検出Poster presentation
- 第63回高分子学会年次大会, May 2014, Japanese, 高分子学会, 名古屋市, Domestic conferenceカーボンナノチューブの可溶化を目指した重合性界面活性剤の開発Poster presentation
- 日本農芸化学会2014年度大会, Mar. 2014, Japanese, 東京, Domestic conference放線菌による 3,4-AHBA 合成とキシロース代謝Oral presentation
- 日本農芸化学会2014年度大会, Mar. 2014, Japanese, 日本農芸化学会, 川崎市, Domestic conference同時糖化発酵による希硫酸前処理バイオマスからのフェニル乳酸生産Oral presentation
- 化学工学会第79年会, Mar. 2014, Japanese, 化学工学会, 岐阜市, Domestic conference新しい放射線治療をめざした過酸化チタンナノ粒子の物性研究Poster presentation
- 第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山, Domestic conferenceバイオマス評価法の開発Poster presentation
- 第16回化学工学会学生発表会(堺大会), Mar. 2014, Japanese, 化学工学会, 大阪市, Domestic conferenceタンパク質提示酵母の表層における親和力解析Oral presentation
- 第8回日本ゲノム微生物学会年会, Mar. 2014, Japanese, 日本ゲノム微生物学会, 世田谷区, Domestic conferenceタンパク高生産放線菌のゲノム変異解析と因子探索Poster presentation
- 日本農芸化学会2014年度大会, Mar. 2014, Japanese, 日本農芸化学会, 川崎市, Domestic conferenceセルロース系バイオマスからの物質生産を可能にしたスーパー麹菌の開発Oral presentation
- 日本農芸化学会2014年度大会, Mar. 2014, Japanese, 日本農芸化学会, 川崎市, Domestic conferenceコリネ型細菌を用いた糖を原料とするGABAの生産Oral presentation
- 化学工学会第79年会, Mar. 2014, Japanese, 化学工学会, 岐阜市, Domestic conferenceイオン液体を用いたリグノセルロースのトータルリファイナリーOral presentation
- 化学工学会第79年会, Mar. 2014, Japanese, 化学工学会, 岐阜市, Domestic conferenceイオン液体と酸を用いた新規バイオマス前処理法の開発と応用Poster presentation
- 第4回イオン液体研究会, Nov. 2013, Japanese, イオン液体研究会, 横浜市, Domestic conference低細胞毒性イオン液体により前処理した木質系バイオマスからの同時糖化発酵Poster presentation
- 第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference放線菌による芳香族化合物ホモゲンチジン酸の生産Poster presentation
- 第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference同時糖化発酵によるセルロースからのフェニル乳酸生産Poster presentation
- 第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference低細胞毒性イオン液体により前処理した木質系バイオマスからの同時糖化発酵Poster presentation
- 第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference低コストイオン液体によるエタノール製造プロセスの提案Poster presentation
- 第62回高分子討論会, Sep. 2013, Japanese, 高分子学会, 金沢市, Domestic conference新しい重合性界面活性剤を用いた難溶性ナノ材料可溶化技術の開発Oral presentation
- 第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference上皮成長因子受容体を特異的に認識するAffibody提示バイオナノカプセルの開発Poster presentation
- 日本農芸化学会関西;中四国;西日本支部合同大会(第481回講演会), Sep. 2013, Japanese, 日本農芸化学会, 広島市, Domestic conference酸触媒イオン液体前処理を用いた新規バイオプロセス構築Oral presentation
- 日本農芸化学会関西;中四国;西日本支部合同大会(第481回講演会), Sep. 2013, Japanese, 日本農芸化学会, 広島市, Domestic conference麹菌Aspergillus oryzae によるL-乳酸の生産Oral presentation
- 平成25年度繊維学会秋季研究発表会, Sep. 2013, Japanese, 名古屋市, Domestic conferenceCharacterization of cellulose nanofiber sheets from various grass plantsPoster presentation
- 第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference稲わら希硫酸処理液のナノフィルトレーションによる糖濃縮Poster presentation
- 第31回日本植物細胞分子生物学会大会;シンポジウム, Sep. 2013, Japanese, 日本植物細胞分子生物学会, 札幌市, Domestic conference易糖化性を示すイネの探索Oral presentation
- 化学工学会第45回秋季大会, Sep. 2013, Japanese, 化学工学会, 岡山市, Domestic conferenceバイオリファイナリーを目指したイオン液体の活用Poster presentation
- 第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conferenceバイオマス分解性向上を目的とする酸導入イオン液体を用いた新規前処理法開発Poster presentation
- 第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conferenceナノ粒子とX線照射を併用した深部ガンの非侵襲的治療法の開発Poster presentation
- 第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conferenceコリネ型細菌を用いたプロトカテク酸の生産Poster presentation
- セルロース学会第20回年次大会, Jul. 2013, Japanese, 京都市, Domestic conferenceCharacterization of cellulose nanofiber sheets from various grass plantsPoster presentation
- 第2回JACI/GSC 講演会, Jun. 2013, Japanese, 新化学技術推進協会, 大阪市, Domestic conference植物源を異にするセルロースナノファイバーシートの構造と物性Poster presentation
- 第2回JACI/GSC 講演会, Jun. 2013, Japanese, 新化学技術推進協会, 大阪市, Domestic conferenceイオン液体を用いたバイオマスの前処理と同時糖化発酵Poster presentation
- 化学工学会 第78年会, Mar. 2013, Japanese, 化学工学会, 大阪大学, Domestic conference重合性界面活性剤による難溶性材料可溶化技術の開発Oral presentation
- 日本化学会第93春季年会, Mar. 2013, Japanese, 日本化学会, 草津市, Domestic conference原子間力顕微鏡を用いた細胞表層解析法および細胞応答解析法の開発Oral presentation
- 化学工学会第78年会, Mar. 2013, Japanese, 化学工学会, 豊中市, Domestic conferenceEthanol production from ionic liquid pretreated biomass by recombinant yeastOral presentation
- 第3回イオン液体討論会, Dec. 2012, Japanese, イオン液体研究会, 那覇市, Domestic conferenceコリン系イオン液体と超音波を組み合わせた木質系バイオマス前処理Oral presentation
- 第3回イオン液体討論会, Dec. 2012, Japanese, イオン液体研究会, 那覇市, Domestic conferenceイオン液体耐性セルラーゼの単離と酵素特性解析Oral presentation
- International Joint Symposium on Single-Cell Analysis (The 6th International Workshop on Approaches to Single-Cell Analysis & The 8th International Forum on Post-Genome Technologies), Nov. 2012, English, The Society for Single-Cell Surveyor, 京都市, International conferenceInteraction survey between GPCR and ligand on the surface of yeast by AFMPoster presentation
- 第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference放線菌バイオマス分解酵素ライブラリの構築及び新規高分泌シグナルの探索Oral presentation
- 酵素工学研究会第68回講演会, Oct. 2012, Japanese, 酵素工学研究会, 文京区, Domestic conferenceピリジニウム系イオン液体による結晶性セルロースのin situ 酵素糖化Poster presentation
- 第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conferenceエンドグルカナーゼ分泌生産型放線菌によるセルロースからの安息香酸生産Oral presentation
- 酵素工学研究会第68回講演会, Oct. 2012, Japanese, 酵素工学研究会, 文京区, Domestic conferenceエンドグルカナーゼ分泌生産型放線菌によるセルロースからの安息香酸生産Poster presentation
- 第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conferenceイオン液体耐性セルラーゼの探索と機能解析Oral presentation
- 酵素工学研究会第68回講演会, Oct. 2012, Japanese, 酵素工学研究会, 文京区, Domestic conferenceイオン液体耐性セルラーゼの単離と酵素特性解析Poster presentation
- 第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conferenceイオン液体前処理による木質系バイオマスからのエタノール生産Oral presentation
- 第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conferenceイオン液体前処理とキシロース資化性酵母株を用いたバイオマス糖化発酵プロセスの効率化Oral presentation
- The 18th Symposium of Young Asian Biochemical Engineers' Community (YABEC2012), Oct. 2012, English, Society for Biological Engineering, Tokushima, Japan, International conferenceGenetic engineering of valine biosynthesis for isobutanol production in Saccharomyces cerevisiaeOral presentation
- The 18th Symposium of Young Asian Biochemical Engineers' Communyty, Oct. 2012, English, ㈳日本生物工学会, 徳島市, International conferenceDirect ethanol production from lignocellulosic biomass pretreated with ionic liquid from cellulase-displaying yeastPoster presentation
- 第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conferenceCell SELEX 法により選抜したヒト肝臓由来がん細胞に対するDNA アプタマーの評価Oral presentation
- The 15th International Biotechnology Symposium (IBS 2012), Sep. 2012, English, The Korean Society for Biotechnology and Bioengineering, Deague, Korea, International conferenceComplex carriers of affibody-displaying bio-nanocapsule and composition-varied liposomes for HER2-expressing breast cancer cell-specific protein deliveryOral presentation
- Metabolic EngineeringⅨ, Jun. 2012, English, Engineering Conferences International, Biarritz, France, International conferenceGenetic engineering to enhance the Ehrlich pathway and alter carbon flux for increased isobutanol production by Saccharomyces cerevisiaeOral presentation
- 21世紀を拓くバイオテクノロジーシンポジウム, Jun. 2012, Japanese, YABEC2012実行委員会, 徳島市, Domestic conferenceAFMを用いた新規核酸分子の探索Oral presentation
- 第72回分析化学討論会, May 2012, Japanese, 日本分生化学会, 鹿児島市, Domestic conferenceラジカルを発生する金属酸化物粒子に対するXAFSによる非破壊状態分析Oral presentation
- The 12th Japan-China-Korea Joint Symposium on Enzyme Engineering, May 2012, English, 酵素工学研究会, 金沢市, International conferenceDNA aptamer screening and evaluation by AFMOral presentation
- 化学工学会第77年会, Mar. 2012, Japanese, 化学工学会, 東京, Domestic conference動的周期操作を用いたエタノール連続発酵プロセスにおける分岐現象Oral presentation
- 日本農芸化学会2012年度大会, Mar. 2012, Japanese, 日本農芸化学会, 京都市, Domestic conference麹菌Aspergillus oryzaeを用いたラクダ科動物由来一本鎖抗体可変部位VHH分泌生産系の構築Oral presentation
- 日本化学会第92春季年会, Mar. 2012, Japanese, 日本化学会, 横浜市, Domestic conference原子間力顕微鏡を用いた細胞表層におけるレセプター・リガンド間相互作用測定系の構築Oral presentation
- 日本農芸化学会2012年度大会, Mar. 2012, Japanese, 日本農芸化学会, 京都市, Domestic conference海洋性超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの構造安定性Oral presentation
- 日本農芸化学会2012年度大会, Mar. 2012, Japanese, 日本農芸化学会, 京都市, Domestic conference海洋性超好熱菌Thermotoga neapolitana由来β-1,3-キシラナーゼの耐熱性を導く要因の解析Oral presentation
- 化学工学会第77年会, Mar. 2012, Japanese, 化学工学会, 新宿区, Domestic conferenceピリジニウム系イオン液体による結晶性セルロースの前処理・酵 素糖化・高速発酵(その2)Oral presentation
- 化学工学会第77年会, Mar. 2012, Japanese, 化学工学会, 新宿区, Domestic conferenceピリジニウム系イオン液体による結晶性セルロースの前処理・酵素糖化・高速発酵(その1)Oral presentation
- 日本化学会第92春季年会, Mar. 2012, Japanese, 日本化学会, 横浜市, Domestic conferenceソマトスタチンに対する新規DNA アプタマーの選抜およびセンシングへの応用Oral presentation
- 化学工学会第77年会, Mar. 2012, Japanese, 化学工学会, 新宿区, Domestic conferenceセルロースからの高効率一段階エタノール発酵を可能にする高機能酵母の創製Oral presentation
- 日本農芸化学会2012年度大会, Mar. 2012, Japanese, 日本農芸化学会, 京都市, Domestic conferenceコリネ型細菌を用いたプトレシンの生産Oral presentation
- 日本農芸化学会2012年度大会, Mar. 2012, Japanese, 日本農芸化学会, 京都市, Domestic conferenceエンドグルカナーゼ分泌生産型放線菌を用いたセルロースからの有用タンパク質生産Oral presentation
- 化学工学会第77年会, Mar. 2012, Japanese, 化学工学会, 新宿区, Domestic conferenceエンドグルカナーゼ分泌生産型放線菌によるセルロースからの安息香酸生産Oral presentation
- 第2回イオン液体討論会, Dec. 2011, Japanese, イオン液体研究会, 京都市, Domestic conferenceリグノセルロース・リファイナリーにおけるイオン液体の役割Oral presentation
- 第2回イオン液体討論会, Dec. 2011, Japanese, イオン液体研究会, 京都市, Domestic conferenceイオン液体耐性セルラーゼの探索と機能評価Oral presentation
- 第2回イオン液体討論会, Dec. 2011, Japanese, イオン液体研究会, 京都市, Domestic conferenceイオン液体前処理バイオマスを用いたエタノール同時糖化発酵Oral presentation
- 第2回イオン液体討論会, Dec. 2011, Japanese, イオン液体研究会, 京都市, Domestic conferenceイオン液体前処理バイオマスの構造解析およびエタノール発酵Oral presentation
- 第2回イオン液体討論会, Dec. 2011, Japanese, イオン液体研究会, 京都市, Domestic conferenceイオン液体共存下における酵母のキシロース発酵能評価Oral presentation
- 第34回日本分子生物学会年会, Dec. 2011, English, 日本分子生物学会, 横浜市, Domestic conferenceAmplification and smoothing of signal activation from human G-protein-coupled receptor for enriching agonist stimulation in yeastPoster presentation
- 極限環境生物学会2011年度(第12回)年会, Nov. 2011, Japanese, 極限環境生物学会, 長崎市, Domestic conference超好熱性細菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの結晶構造解析Poster presentation
- 極限環境生物学会2011年度(第12回)年会, Nov. 2011, Japanese, 極限環境生物学会, 長崎市, Domestic conference超好熱性細菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの安定性解析Poster presentation
- 第11回糸状菌分子生物学コンファレンス(2011), Nov. 2011, Japanese, 糸状菌分子生物学研究会, 文京区, Domestic conference組換え麹菌生産セルラーゼを用いた広葉樹クラフトパルプの酵素糖化Poster presentation
- 第11回糸状菌分子生物学コンファレンス(2011), Nov. 2011, Japanese, 糸状菌分子生物学研究会, 文京区, Domestic conferenceラクダ由来一本鎖抗体可変部位 VHH の麹菌 Aspergillus oryzae による分泌生産Poster presentation
- 化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋市, Domestic conference放線菌が有する炭素源資化能力を生かしたタンパク質の大量分泌生産Poster presentation
- 第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference組換え酵母による高効率グルタチオン生産Oral presentation
- 第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference生体分子融合ナノ粒子の生物化学工学領域への高度応用に関する研究[Invited]Invited oral presentation
- 第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference麹菌Aspergillus oryzae による超好熱菌Thermotoga neapolitana 由来耐熱性β-1,4- マンナナーゼの生産Oral presentation
- 第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference酵母によるバイオマスからのグルタチオンの直接発酵Oral presentation
- 第29回日本植物細胞分子生物学会大会, Sep. 2011, Japanese, 日本植物細胞分子生物学会, 福岡市, Domestic conference稲わらバイオマス形質の系統間比較Oral presentation
- 化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋市, Domestic conferenceβグルコシダーゼを表層提示したセロオリゴ糖資化性大腸菌の創製Poster presentation
- 第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conferenceセルラーゼ分泌酵母によるイオン液体処理バイオマスからのエタノール生産Oral presentation
- 化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋市, Domestic conferenceセルラーゼ発現最適化二倍体酵母による木質系バイオマスからのエタノール発酵Poster presentation
- 第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conferenceイオン液体処理が結晶性セルロース酵素糖化に与える影響Oral presentation
- 第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conferenceイオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発Oral presentation
- 化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋市, Domestic conferenceCorynebacterium glutamicumを用いた高効率なGABA生産技術の開発Poster presentation
- 第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conferenceBGL 表層提示大腸菌によるセロビオースを基質としたIPA 生産Oral presentation
- 第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conferenceAffibody 提示Bio-nanocapsule を用いたER2 発現癌細胞へのタンパク質送達システムOral presentation
- Defence, Science & Research (DSR 2011) Conference, Aug. 2011, English, Committee of DSR2011, Singapore, International conferenceAromatic chemicals production using phenylalnine ammonia lyase expressing Streptomyces lividansOral presentation
- 第27回日本DDS学会学術集会, Jun. 2011, Japanese, 日本DDS学会, 文京区, Domestic conferenceバイオナノカプセルを用いた細胞特異的ドラッグデリバリーシステムOral presentation
- International Conference on Materials for Asdvanced Technologies 2011, Jun. 2011, English, Materials Research Society Singapore, Singapore, International conferenceKey Monomers Production from Biomass Toward Bio-based Polymers UsingOral presentation
- 第60回高分子学会年次大会, May 2011, Japanese, 高分子学会, 大阪市, Domestic conferenceイオン液佒へのセルロースの溶解とバイオエタノール生産Oral presentation
- 33rd Symposium on Biotechnology for Fuels and Chemicals, May 2011, English, Society for industrial Microbiology, Seattle, USA, International conferenceHomo D-lactic acid fermentation from pentose and xylan by metabolically engineered Lactobacillus plantarumPoster presentation
- 2011 International Meeting of the Microbiological Society of Korea, May 2011, English, The Microbiological Society of Korea, Gwangju, Korea, International conferenceEffective Screening of Optimized Diploid Saccharomyces cerevisiae Strain for Direct Ethanol Production from Cellulosic Materials by Displaying Ratio Adjustment of Cellulolytic EnzymesOral presentation
- 33rd Symposium on Biotechnology for Fuels and Chemicals, May 2011, English, Society for industrial Microbiology, Seattle, USA, International conferenceCinnamic acid production from various carbon sources using phenylalnine ammonia lyase expressing Streptomyces lividansPoster presentation
- E-MRS ICAM IUMRS 2011 Spring Meeting, May 2011, English, European Materials Research Society, Nice, France, International conferenceCharacterization of Titanium oxide nano-particle suspension by XAFS techniqueOral presentation
- 化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference放線菌を用いたあらゆる炭素源からのケイ皮酸生産Oral presentation
- 日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference放線菌によるトランスグルタミナーゼの大量分泌生産に向けた生産条件の検討Oral presentation
- 化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference放線菌によるトランスグルタミナーゼの生産Oral presentation
- 化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference放線菌における表層提示技術に関する研究Oral presentation
- 化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference長期エタノール発酵に向けたアミラーゼ表層提示発現酵母株の創製Oral presentation
- 化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference代謝工学を活用したイソブタノール生産酵母の創製Oral presentation
- 日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference組換え酵母による高効率グルタチオン生産Oral presentation
- 化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference酵母を用いたバイオマス資源からのグルタチオン生産技術の開発Oral presentation
- 化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference酵母を用いたバイオマスからのグルタチオンの生産Oral presentation
- 日本化学会第91春季年会, Mar. 2011, Japanese, 化学工学会, 横浜市, Domestic conference酵素活性を制御するDNAアプタマーの探索Oral presentation
- 日本化学会第91春季年会, Mar. 2011, Japanese, 化学工学会, 横浜市, Domestic conference原子間力顕微鏡を用いた機能性核酸分子の選抜Oral presentation
- 化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference原子間力顕微鏡を用いた機能性核酸分子の選抜Oral presentation
- 日本化学会第91春季年会, Mar. 2011, Japanese, 化学工学会, 横浜市, Domestic conference原子間力顕微鏡を用いたアミノ酸に対する機能性核酸分子・アプタマーの新規選抜法の開発Oral presentation
- 化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference過酸化チタンナノ粒子及び低線量X線照射を併用した深部ガンの非侵襲的治療法の開発Oral presentation
- 化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference過酸化チタンナノ粒子及び低線量X線照射を併用した深部ガンの非侵襲的治療法の開発Oral presentation
- 日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conferenceβグルコシダーゼ表層提示によるセロビオース資化性大腸菌の創製Oral presentation
- 化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conferenceβグルコシダーゼを表層提示したセロビオース資化性大腸菌の創製Oral presentation
- 化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conferenceランダム変異導入による高活性エンドグルカナーゼの創製Oral presentation
- 化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conferenceトランスポゾンタギング法による好塩性細菌Halomonas elongata OUT30018株の浸透圧ストレス応答性遺伝子の探索Oral presentation
- 日本化学会第91春季年会, Mar. 2011, Japanese, 化学工学会, 横浜市, Domestic conferenceソマトスタチンに対する新規DNAアプタマーの選抜Oral presentation
- 日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conferenceコリネ型細菌を用いたGABAの生産Oral presentation
- 日本化学会第91春季年会, Mar. 2011, Japanese, 化学工学会, 横浜市, Domestic conferenceイオン液体共存下でのセルロース系バイオマスからの直接エタノール発酵Oral presentation
- 日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conferenceイオン液体を利用したバイオマスの前処理・糖化・発酵技術Oral presentation
- 化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conferenceイオン液体をベースとしたセルロース系バイオマスの前処理とエタノール発酵プロセスOral presentation
- 化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conferenceイオン液体によるバイオマスの前処理・糖化・発酵技術の開発Oral presentation
- 日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conferenceStreptomyces griseocarneusスフィンゴミエリナーゼCの速度論的解析とMg2+による活性増強メカニズムの解明Oral presentation
- The 5th International Workshop on Approaches to Single-Cell Analysis, Mar. 2011, English, Tokyo, Japan, International conferenceSingle-cell analysis revealed correlation between yeast GPCR signaling and plasmid retentionPoster presentation
- 化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conferenceCorynebacterium glutamicumを用いたGABAの高効率生産技術の開発Oral presentation
- 日本化学会第91春季年会, Mar. 2011, Japanese, 化学工学会, 横浜市, Domestic conferenceAFMを用いた生細胞表層のレセプター・リガンド間相互作用解析と細胞応答の観察Oral presentation
- 芦屋川カレッジ, Feb. 2011, Japanese, 芦屋市公民館, 芦屋市, Domestic conference植物から燃料を作るInvited oral presentation
- 第23回CES21講演会, Feb. 2011, Japanese, 日本分子生物学会, 大阪市, Domestic conference金属酸化ナノ粒子と物理的励起を併用したラジカル発生システムを用いた細胞損傷システムの構築Invited oral presentation
- The 2010 International Chemical Congress of Pacific Basin Societies (Pacifichem 2010), Dec. 2010, English, Honolulu, Hawaii, USA, International conferenceHigh-resolution, quantitative signalling assay for G-protein-coupled receptors by flow cytometryOral presentation
- 第10回 糸状菌分子生物学コンファレンス, Nov. 2010, Japanese, 糸状菌分子生物学研究会, 東広島市, Domestic conference麹菌A. oryzaeによる海洋細菌由来β-1,3-キシラナーゼの生産Poster presentation
- 第10回 糸状菌分子生物学コンファレンス, Nov. 2010, Japanese, 糸状菌分子生物学研究会, 東広島市, Domestic conference麹菌A. oryzaeにより生産した海洋細菌由来β-1,3-キシラナーゼの酵素学的諸性質Poster presentation
- The 16th Symposium of Young Asian Biochemical Engineers' Communyty, Nov. 2010, English, (社)化学工学会, 台湾桃園県, International conferenceSelection of DNA aptamers using Atomic Force MicroscopyOral presentation
- International Conference on Sustainable Biomass (ICSB) 2010, Nov. 2010, English, 不明, Tamsui, Taiwan, International conferenceIonic liquid Pretreatment for Direct Ethanol Production from Cellulosic Material by Cellulase-displaying Arming YeastOral presentation
- 第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference放線菌を用いた様々なタンパクの大量分泌系の構築Poster presentation
- 第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference超好熱菌Thermotoga neapolitana 由来耐熱性β -1,3- キシラナーゼの機能解析Poster presentation
- 第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference麹菌による超好熱菌Thermotoga neapolitana 由来耐熱性β -1,3- キシラナーゼの高分泌生産Poster presentation
- 第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference過酸化チタンナノ粒子及び低線量X 線照射を併用した深部ガンの非侵襲的治療法の開発Poster presentation
- 第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conferenceセルラーゼ発現バランス最適化酵母を用いたセルロースからのエタノール発酵Poster presentation
- 第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conferenceイオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発(その4)Poster presentation
- 第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conferenceイオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発(その3)Poster presentation
- 第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conferenceイオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発(その2)Poster presentation
- 第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conferenceCell SELEX 法によるヒト肝臓由来がん細胞に選択的に結合するDNA アプタマーの選抜Poster presentation
- 第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conferenceAffibody 提示ナノカプセルを用いたHER2 発現癌細胞特異的な薬物送達Poster presentation
- 第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference1細胞アッセイ技術に基づくGPCR 解析、アゴニスト探索法の開発Oral presentation
- 化学工学会第42回秋季大会, Sep. 2010, Japanese, (社)化学工学会, 京都市, Domestic conference力学的指標による生細胞表面におけるリガンド‐受容体の相互作用解析Poster presentation
- 2010年度日本放線菌学会大会, Sep. 2010, Japanese, 日本放線菌学会, 東京都 江戸川区, Domestic conference放線菌を用いた様々なタンパクの大量分泌系の構築Oral presentation
- 日本応用糖質科学会 平成22年度大会(第59回), Sep. 2010, Japanese, 日本応用糖質科学会, 静岡市, Domestic conference超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの発現および諸性質Oral presentation
- 日本応用糖質科学会 平成22年度大会(第59回), Sep. 2010, Japanese, 日本応用糖質科学会, 静岡市, Domestic conference麹菌(Aspergillus oryzae)を宿主とした超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの分泌高生産Oral presentation
- 化学工学会第42回秋季大会, Sep. 2010, Japanese, (社)化学工学会, 京都市, Domestic conference高効率バイオディーゼル生産を目的としたWhole-cell biocatalystによる新規リパーゼ複合系の開発Poster presentation
- 化学工学会第42回秋季大会, Sep. 2010, Japanese, (社)化学工学会, 京都市, Domestic conference酵母によるエタノール発酵に及ぼす撹拌剪断効果Poster presentation
- 化学工学会第42回秋季大会, Sep. 2010, Japanese, (社)化学工学会, 京都市, Domestic conference機能性酵母によるバイオエタノール生産プロセスの強化Poster presentation
- 化学工学会第42回秋季大会, Sep. 2010, Japanese, (社)化学工学会, 京都市, Domestic conferenceaffibody提示バイオナノカプセルを用いたタンパク質デリバリーシステムPoster presentation
- RENEWABLE ENERGY 2010, Jun. 2010, English, International Solar Energy Society, 横浜市, International conferenceConstruction of consolidated bioprocesses for production of bio-fuels and chemicals by using cell surface engineered microbial cellsOral presentation
- Industrial Biotechnology International Conference 2010, Apr. 2010, English, The Italian Association of Chemical Engineering BIOTECH WORKING GROUP, Milano, Italy, International conferenceIntensification of Bioethanol Fermentation by Recombinant Yeast with Xylose Isomerase PathwayOral presentation
- 化学工学会第75年会, Mar. 2010, Japanese, (社)化学工学会, 鹿児島市, Domestic conference放線菌を用いた様々なタンパクの大量分泌系の構築Oral presentation
- 化学工学会第75年会, Mar. 2010, Japanese, (社)化学工学会, 鹿児島市, Domestic conference非侵襲性がん治療に向けた高機能性二酸化チタンナノ粒子の開発Oral presentation
- 化学工学会第75年会, Mar. 2010, Japanese, (社)化学工学会, 鹿児島市, Domestic conference生デンプンからの効率的な繰返し発酵を達成するアミラーゼ発現新規酵母株の創製Oral presentation
- 日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference細胞表層工学技術を用いたヘミセルロース系バイオマスからのエタノール生産Oral presentation
- 日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference酵母を用いた生デンプンからの高効率な機能性ペプチド生産Oral presentation
- 日本化学会第90春季年会, Mar. 2010, Japanese, 日本化学会, 東大阪市, Domestic conference原子間力顕微鏡を用いた細胞表層におけるリガンド・レセプター間相互作用測定系の構築Oral presentation
- 日本化学会第90春季年会, Mar. 2010, Japanese, 日本化学会, 東大阪市, Domestic conference原子間力顕微鏡を用いた機能性核酸分子・アプタマーの新規選抜法の開発Oral presentation
- 日本化学会第90春季年会, Mar. 2010, Japanese, 日本化学会, 東大阪市, Domestic conference機能性酵母によるイオン液体前処理セルロースからのバイオエタノール生産Oral presentation
- 日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conferenceバイオマスからの燃料・化学品生産を目指した細胞工場の創製Oral presentation
- 化学工学会第75年会, Mar. 2010, Japanese, (社)化学工学会, 鹿児島市, Domestic conferenceセルラーゼ表層提示酵母を用いたイオン液体前処理セルロースからの直接エタノール発酵Oral presentation
- 日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conferenceカクテルδインテグレーション法によるセルラーゼ発現バランス最適化酵母の創製Oral presentation
- 日本化学会第90春季年会, Mar. 2010, Japanese, 日本化学会, 東大阪市, Domestic conferenceDNAアプタマーを用いた酵素活性制御系の構築Oral presentation
- 日本化学会第90春季年会, Mar. 2010, Japanese, 日本化学会, 東大阪市, Domestic conferenceAFMを用いたアプタマーによるタンパク質の検出・定量方法Oral presentation
- 第32回日本分子生物学会年会, Dec. 2009, Japanese, 日本分子生物学会, 横浜市, Domestic conferenceセルラーゼ提示超分子複合体提示酵母によるバイオマスの効率的分解Invited oral presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceScreening of DNA aptamers that recognize amino acid by AFM-SELEX strategyPoster presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceProtein production for Bio-refinery strategyPoster presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceProduction of biodiesel fuel in ionic liquids catalyzed by whole-cell biocatalystsOral presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceNew SELEX strategy for screening of DNA aptamer by AFMOral presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceInvestigation of the interaction between GPCR and ligand by AFM equipped with bio-molecule modified cantileverPoster presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceEngineering of endoglucanase expression system for Corynebacterium glutamicumPoster presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceEfficient repeated batch fermentation from raw starch by novel yeast expressing transporter gene and amylase genesPoster presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceEfficient homo D-lactic acid production from xylosePoster presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceEfficient ethanol production from xylose by mated diploid Saccharomyces cerevisiaePoster presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceEfficient D-lactic acid production from raw starchPoster presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceEfficient and practical ethanol production from high yield rice by amylase expressing Saccharomyces cerevisiaePoster presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceDirect fermentation of cellulosic materials to ethanol using yeast strains codisplaying three types of cellulolytic enzymePoster presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceDevelopment of novel cell-surface display system of Aspergillus oryzae using chitin binding domainPoster presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceBioethaol fermentation from mixed sugar by the recombinant yeast with xyloseisomerase pathwayPoster presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceBioethanol production from mixed sugars using sugar uptake ability enhanced yeast strain by overexpression of transportersPoster presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceBiodiesel production using whole-cell biocatalyst of Aspergillus oryzae coexpression lipasesPoster presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceAntibody-immobilized TiO2 nanoparticles for cancer therapyPoster presentation
- APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conferenceAffibody displaying bionanocapsules for HER2 specific drug deliveryPoster presentation
- バイテクノロジー講演会, Oct. 2009, Japanese, バイオテクノロジー研究会, 福井市, Domestic conferenceセルロース資源からのバイオエタノール生産技術Invited oral presentation
- 化学工学会第41回秋季大会, Sep. 2009, Japanese, 化学工学会, 広島大学, Domestic conference糖代謝ネットワークを強化した酵母による混合糖からのバイオエタノール生産Oral presentation
- 第61回日本生物工学会大会, Sep. 2009, Japanese, (社)日本生物工学会, 名古屋市, Domestic conference生デンプンから高効率にエタノールを生産する新規酵母の創製Oral presentation
- 第41回 化学工学会秋季大会, Sep. 2009, Japanese, (社)化学工学会, 東広島市, Domestic conference菌体触媒を用いたバイオディーゼル生産におけるイオン液体の利用Oral presentation
- 第61回日本生物工学会大会, Sep. 2009, Japanese, (社)日本生物工学会, 名古屋市, Domestic conference癌細胞標的化を目指したAffibody 提示ナノカプセルの開発Oral presentation
- 第61回日本生物工学会大会, Sep. 2009, Japanese, (社)日本生物工学会, 名古屋市, Domestic conference遺伝子組換え麹菌を用いたタンパク質大量分泌生産技術の開発Oral presentation
- 化学工学会第41回秋季大会, Sep. 2009, Japanese, 化学工学会, 東広島市, Domestic conferenceヘマトコッカス藻の高密度培養に対する二酸化チタン-超音波励起法の適用Poster presentation
- Progress on Post Genome Technologies, Sep. 2009, English, Tsinghua, Southeast, Nanjing, Northwest University, Beijing, China, International conferenceSelection of DNA aptamers using atomic force microscopyOral presentation
- 第61回日本生物工学会大会, Sep. 2009, Japanese, (社)日本生物工学会, 名古屋市, Domestic conferencePorin を用いたコリネ菌新規細胞表層提示技術の開発Oral presentation
- SIM2009 Annual Meeting and Exhibition, Jul. 2009, English, Society for Industrial Microbiology, Toronto, Canada, International conferenceDevelopment of environmentally benign and consolidated process for efficient production of cellulosic ethanol[Invited]Invited oral presentation
- (財)不二たん白質研究振興財団第12回研究報告会, Jun. 2009, Japanese, (財)不二たん白質研究振興財団, 豊中市, Domestic conference大豆イソフラボンからのイソフラボンアグリコンの効率的生産に適した新規βグルコシダーゼの開発Oral presentation
- 化学工学会 第75年会, Mar. 2009, Japanese, 化学工学会, 神奈川県横浜市, Domestic conference放線菌遺伝子組み換え系のタンパク質分泌生産への適用Oral presentation
- 化学工学会 第74年会, Mar. 2009, Japanese, 化学工学会, 神奈川県横浜市, Domestic conference非侵襲性がん治療のためのタンパク質修飾ナノ粒子の開発Oral presentation
- 農芸化学会2009年度大会, Mar. 2009, Japanese, 日本農芸化学会, 福岡県福岡市, Domestic conferenceバイオエタノール生産を目指したスーパー酵母の創製Oral presentation
- 日本草地学会, Mar. 2009, Japanese, 日本草地学会, 神奈川県藤沢市, Domestic conferenceソフトセルロース資源からのエタノール発酵技術の開発Invited oral presentation
- 農芸化学会2009年度大会, Mar. 2009, Japanese, 日本農芸化学会, 福岡県福岡市, Domestic conferenceキシロース資化性遺伝子組換え酵母の代謝プロファイリング解析Poster presentation
- 農芸化学会2009年度大会, Mar. 2009, Japanese, 日本農芸化学会, 福岡県福岡市, Domestic conferenceキシロースからの高効率エタノール生産を目指した宿主酵母株の検討Poster presentation
- 農芸化学会2009年度大会, Mar. 2009, Japanese, 日本農芸化学会, 福岡県福岡市, Domestic conferenceアミラーゼ発現酵母による生デンプンからの効率的な繰り返し発酵プロセスの開発Poster presentation
- 化学工学会 第74年会, Mar. 2009, Japanese, 化学工学会, 神奈川県横浜市, Domestic conferenceTiO&2&ナノ粒子と超音波を併用した新規がん治療法構築のための基礎的検討Oral presentation
- 第31回日本分子生物学会年会;第81回日本生化学会大会 合同大会, Dec. 2008, Japanese, 日本分子生物学学会, 日本生化学会, 神戸市, Domestic conference未来バイオ燃料生産用スーパー(CBP)微生物の開発Oral presentation
- 第31回日本分子生物学会年会・第82回日本生化学会大会 合同大会, Dec. 2008, Japanese, 日本分子生物学学会, 日本生化学会, 神戸市, Domestic conference二酸化チタンナノ粒子による活性酸素非依存的細胞死誘導Oral presentation
- 若手フロンティア研究会2008, Dec. 2008, Japanese, 神戸大学研究基盤センター, 神戸市, Domestic conference原子間力顕微鏡を用いたアプタマーの選抜Poster presentation
- 若手フロンティア研究会2008, Dec. 2008, Japanese, 神戸大学研究基盤センター, 神戸市, Domestic conference温度応答性磁性ナノ粒子を用いた迅速かつ高効率なアフィニティー分子の選択法の確立Poster presentation
- 4th International Symposium on Biocatalysis and Biotechnology, Nov. 2008, English, International Society of Biocatalysis and Biotechnology, Taipei, Taiwan, International conferenceProduction of bio-fuels and chemicals from biomass by cell surface engineered yeast strains[Invited]Invited oral presentation
- 4th Korea-Japan Workshop on Combinatorial Bioengineering, Nov. 2008, English, Association of Combinatorial Bioengineering, Kookmin University, Seoul, International conferenceMethylester Synthesis By Fungi Immobilized BSPs in Ionic LiquidPoster presentation
- 4th Korea-Japan Workshop on Combinatorial Bioengineering, Nov. 2008, English, Association of Combinatorial Bioengineering, Seoul, Korea, International conferenceEfficient and direct ethanol production from raw starch by d-integrant polyploid fusants of Saccharomyces cerevisiaePoster presentation
- 4th Korea-Japan Workshop on Combinatorial Bioengineering, Nov. 2008, English, Association of Combinatorial Bioengineering, Seoul, Korea, International conferenceBiorefinery strategy by cell surface engineered yeast strainsPoster presentation
- 第17回日本バイオイメージング学会, Oct. 2008, Japanese, 日本バイオイメージング学会, 千葉市, Domestic conference特異性改変型バイオナノカプセルを用いたバイオイメージング及びDDSへの応用Oral presentation
- International Workshop on Process Intensification 2008, Oct. 2008, English, 化学工学会粒子流体プロセス部会, 化学工学会システム・情報・シミュレーション部会, 化学工学会反応工学部会, 化学工学会マイクロ化学研究会, Tokyo Institute of Technology, International conferenceImprovement of Yeast Xylose Uptake Ability for Efficient Bioethanol ProductionPoster presentation
- 化学工学会 第40回 秋季大会, Sep. 2008, Japanese, 化学工学会, 宮城県仙台市, Domestic conferenceセルラーゼ発現酵母によるセルロースからのバイオエタノール生産Poster presentation
- 第60回 日本生物工学会2008年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conference無蒸煮デンプンから効率的にエタノールを生産する高機能酵母の創製Oral presentation
- 第60回 日本生物工学会2010年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conference遺伝子組み換え放線菌を用いたセルラーゼ発現システムの構築Oral presentation
- 第60回 日本生物工学会2009年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conferenceリン脂質代謝酵素のイオン液体中での反応解析Oral presentation
- 第60回 日本生物工学会2008年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conferenceキチン結合ドメインを用いた麹菌細胞表層提示技術の開発Oral presentation
- 第60回 日本生物工学会2008年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conferenceStreptomyces griseocarneus NBRC13471由来スフィンゴミエリナーゼC の遺伝子クローニングと発現Oral presentation
- American Society for Microbiology 108th General Meeting, Jun. 2008, English, American Society for Microbiology, Boston, USA, International conferenceOxidative-stress-induced metabolite production with sonocatalytic formation of reactive oxygen speciesPoster presentation
- 化学工学会第73年会, Mar. 2008, Japanese, 化学工学会, 浜松市, Domestic conferenceフローサイトメーター(FCM)を利用した酵母GPCRシグナリング解析技術Oral presentation
- 第6回最先端バイオテクノロジー若手発表会, Feb. 2008, Japanese, 化学工学会関西支部化学工学会バイオ部会, 西宮市, Domestic conference酵母GPCRシグナリングにおけるフローサイトメトリー解析技術Poster presentation
- 第6回 最先端バイオテクノロジー公開セミナー, Feb. 2008, Japanese, 化学工学会関西支部、化学工学会バイオ部会, 西宮, Domestic conferenceストレス応答を利用したHaematococcus pluvialisによるアスタキサンチン生産の高効率化Oral presentation
- ISSS2007, Dec. 2007, English, ソノケミストリー学会, 京都, International conferenceUltrasonic facilitation of OH radical generation in the presence of TiO2Oral presentation
- ISSS2007, Dec. 2007, English, ソノケミストリー学会, 京都, International conferenceThe cancer cell injury by combination of protein displayed titanium dioxide nanoparticle and ultrasound irradiationOral presentation
- ISSS2007, Dec. 2007, English, ソノケミストリー学会, 京都, International conferenceConstruction of biomolecule-immobilized TiO2 nanoparticle for applying to new cell injuring method with ultrasound irradiationOral presentation
- ISSS2007, Dec. 2007, English, ソノケミストリー学会, 京都, International conferenceAnalysis of Sonodynamic Sterilization of Escherichia coli at the Surface of TiO2Oral presentation
- ISSS2007, Dec. 2007, English, ソノケミストリー学会, 京都, International conferenceAnalysis of H2O2 formation induced by ultrasonic irradiation with TiO2. KOral presentation
- YABEC2007, Oct. 2007, English, Young Asian Biochemical Engineers Community, Seoul, Korea, International conferenceOxidative treatment of H. pluvialis with the sonocatalytic reactionPoster presentation
- YABEC2007, Oct. 2007, English, YABEC, Souel 韓国, International conferenceApplication of biomolecule-immobilized TiO2 nanoparticle for chemical compound degradation and mammalian cell killingOral presentation
- 日本生物工学会第59回大会, Sep. 2007, Japanese, 日本生物工学会, 東広島, Domestic conference放線菌を宿主とするリン脂質代謝酵素の変異体解析Oral presentation
- 日本生物工学会第59回大会, Sep. 2007, Japanese, 日本生物工学会, 東広島, Domestic conference放線菌Streptomyces lividans における分泌シグナルのタンパク生産に及ぼす影響解析Oral presentation
- 第59回生物工学会大会, Sep. 2007, Japanese, 日本生物工学会, 広島, Domestic conference二酸化チタン-超音波励起法を利用したヘマトコッカス藻によるアスタキサンチン生産の誘導Oral presentation
- ECB13, Sep. 2007, English, ECB, バルセロナ スペイン, International conferenceOver-expression system of secretory phospholipase D by Streptomyces lividansOral presentation
- The 2nd International Workshop on Approaches to Single-Cell Analysis, Sep. 2007, English, 東京, International conferenceNew SELEX strategy for screening of DNA aptamer by AFM.Oral presentation
- ECB13, Sep. 2007, English, ECB, バルセロナ スペイン, International conferenceInduction of astaxantin biosynthesis in H. pluvialis using TiO2 and ultrasoundsOral presentation
- 13th European Congress on Biotechnology, Sep. 2007, English, European Federation of Biotechnology, Barcelona, Spain, International conferenceInduction of astaxanthin biosynthesis in H. pluvialis using TiO2 and ultrasoundsPoster presentation
- 2nd International Congress on Ionic Liquids (COIL-2), Aug. 2007, English, 横浜, International conferencePhospholipid conversion in ionic liquid by phospholipase DOral presentation
- 化学工学会第72年会, Mar. 2007, Japanese, 化学工学会, 京都, Domestic conference放線菌の新規タンパク質発現系構築に関する研究Oral presentation
- 化学工学会第72年会, Mar. 2007, Japanese, 化学工学会, 京都, Domestic conference組織ターゲティング機能を有する二酸化チタンナノ粒子によるがん細胞障害Oral presentation
- 日本化学会第87春季年会, Mar. 2007, Japanese, 日本化学会, 吹田, Domestic conferenceリン脂質代謝酵素の酵素活性に及ぼすイオン液体の効果Oral presentation
- 日本化学会第87春季年会, Mar. 2007, Japanese, 日本化学会, 吹田, Domestic conference8OH-dGを認識するDNAアプタマーの機能評価Oral presentation
- 9th Japan-China-Korea Joint symposium on Enzyme Engineering, Nov. 2006, English, 酵素工学研究会, 大津, International conferenceStructural analysis of 8OH-dG DNA aptamerOral presentation
- YABEC2006, Nov. 2006, English, YABEC, 高雄, International conferenceOver-expression system for secretory enzyme by Streptomyces lividansOral presentation
- 9th Japan-China-Korea Joint symposium on Enzyme Engineering, Nov. 2006, English, 酵素工学研究会, 大津, International conferenceEffect of solvent and ionic liquid on phospholipid conversion in bi-phase system by phospholipase DOral presentation
- 9th Japan-China-Korea Joint symposium on Enzyme Engineering, Nov. 2006, English, 酵素工学研究会, 大津, International conferenceConstruction of secretional expression system by Streptomyces lividansOral presentation
- 第15回ソノケミストリー討論会, Oct. 2006, Japanese, ソノケミストリー学会, 金沢, Domestic conference二酸化チタン超音波触媒法を応用した有害微生物殺菌システムOral presentation
- 第15回ソノケミストリー討論会, Oct. 2006, Japanese, ソノケミストリー学会, 金沢, Domestic conference二酸化チタン超音波触媒法による細胞死誘導Oral presentation
- 第15回ソノケミストリー討論会, Oct. 2006, Japanese, ソノケミストリー学会, 金沢, Domestic conference二酸化チタン・超音波法によるOHラジカルの生成と溶存アルゴンの促進効果Oral presentation
- 第15回ソノケミストリー討論会, Oct. 2006, Japanese, ソノケミストリー学会, 金沢, Domestic conference二酸化チタンへの超音波照射による過酸化水素生成機構の解析Oral presentation
- 日本生物工学会第58回大会, Sep. 2006, Japanese, 日本生物工学会, 豊中, Domestic conference放線菌を用いたホスホリパーゼD酵素の遺伝子組み換え生産の培養特性解析に関する研究Oral presentation
- 化学工学会第38回秋季大会, Sep. 2006, Japanese, 化学工学会, 福岡, Domestic conference二酸化チタン・超音波法によるOHラジカルの生成と溶存アルゴンの促進効果Oral presentation
- 日本生物工学会第58回大会, Sep. 2006, Japanese, 日本生物工学会, 豊中, Domestic conference二酸化チタン・超音波触媒法によるがん細胞障害と増殖抑制Oral presentation
- 日本生物工学会第58回大会, Sep. 2006, Japanese, 日本生物工学会, 豊中, Domestic conference組織認識能を持つ二酸化チタン内包リポソームの創製Oral presentation
- 日本生物工学会第58回大会, Sep. 2006, Japanese, 日本生物工学会, 豊中, Domestic conference生体ストレスマーカー分子8OH-dGを認識するDNAアプタマーに関する研究Oral presentation
- 化学工学会第38回秋季大会, Sep. 2006, Japanese, 化学工学会, 福岡, Domestic conference8OH-dGを認識するDNAアプタマーに関する基礎研究Oral presentation
- 化学工学会第71年会, Mar. 2006, Japanese, 化学工学会, 東京, Domestic conference二酸化チタン・超音波触媒法によるがん細胞傷害と増殖抑制Oral presentation
- 化学工学会第71年会, Mar. 2006, Japanese, 化学工学会, 東京, Domestic conference組織認識能をもつ二酸化チタン内包リポソームの創製Oral presentation
- 化学工学会第71年会, Mar. 2006, Japanese, 化学工学会, 東京, Domestic conferenceリン脂質代謝酵素の蓋構造変化における活性相関に関する研究(II)Oral presentation
- 日本生物工学会第57回大会, Nov. 2005, Japanese, 日本生物工学会, 筑波, Domestic conference放線菌Streptomyces cinnamoneum由来発現プロモーターを用いたタンパク質発現系構築に関する研究Oral presentation
- 日本生物工学会第57回大会, Nov. 2005, Japanese, 日本生物工学会, 筑波, Domestic conferenceリン脂質代謝酵素ホスホリパーゼDの蓋構造の活性相関に関する研究Oral presentation
- 第14回ソノケミストリー討論会, Oct. 2005, Japanese, ソノケミストリー学会, 豊橋, Domestic conference有害微生物の超音波殺菌に対する二酸化チタンの増強効果Oral presentation
- 化学工学会第70年会, Mar. 2005, Japanese, 化学工学会, 名古屋, Domestic conference放線菌を宿主とするタンパク質発現系の構築Oral presentation
- 化学工学会第70年会, Mar. 2005, Japanese, 化学工学会, 名古屋, Domestic conferenceリン脂質代謝酵素の蓋構造変化における活性相関に関する研究Oral presentation
- Bioseparation2004, Oct. 2004, English, 化学工学会, 福岡, International conferenceScreening of DNA aptamer that recognizes nucleotide derivativeOral presentation
- 10thAPCChE, Oct. 2004, English, 化学工学会, 北九州, International conferenceInvestigation of the Catalytic Function of Phospholipase D by Site Directed MutagenesisOral presentation
- 10thAPCChE, Oct. 2004, English, 化学工学会, 北九州, International conferenceCell Disinfection by Ultrasonic Irradiation with TiO2 PhotocatalystOral presentation
- 化学工学会山口下関大会, Sep. 2004, Japanese, 化学工学会, 下関, Domestic conference低分子化合物を認識するDNAアプタマーの取得とそのバイオセンサーへの応用Oral presentation
- 日本生物工学会第56回大会, Sep. 2004, Japanese, 日本生物工学会, 名古屋, Domestic conference生理活性物質を認識するDNAアプタマーの探索とその機能評価Oral presentation
- YABEC2004, Sep. 2004, English, YABEC, 大阪, International conferenceScreening of DNA aptamer that recognizes nucleotide derivativeOral presentation
- 化学工学会第69年会, Apr. 2004, Japanese, 化学工学会, 堺, Domestic conference有機溶媒感受性プロモーターを用いた水質評価法の構築Oral presentation
- 1st PRICPS, Apr. 2004, English, 日本蛋白質学会, 横浜, International conferenceInvestigation of catalytic function of phospholipase D enzyme by site directed mutagenesisOral presentation
- 化学工学会第69年会, Apr. 2004, English, 化学工学会, 堺, Domestic conferenceDisinfection of Legionella by sonocatalytic irradiation with TiO2 photocatalystOral presentation
- 化学工学会京都大会, Dec. 2003, Japanese, 化学工学会, 京都, Domestic conference新規ラジカル生成法の環境浄化と医療への応用Oral presentation
- YABEC2003, Nov. 2003, English, YABEC, 済洲島, International conferenceScreening of DNA aptamer that recognizes phenol molecule.Oral presentation
- 第12回ソノケミストリー討論会, Oct. 2003, Japanese, ソノケミストリー学会, 福岡, Domestic conference二酸化チタン・超音波照射法による大腸菌殺菌メカニズムの解析Oral presentation
- 日本生物工学会第55回大会, Sep. 2003, Japanese, 日本生物工学会, 熊本, Domestic conference放線菌由来リン脂質代謝酵素の変異体の機能解析Oral presentation
- European Conference of Biotechnology (ECB) 11th, Aug. 2003, English, ECB, バーゼル・スイス, International conferenceInvestigation of catalytic function of phospholipase D enzyme by site directed mutagenesisOral presentation
- 化学工学会第68年会, Mar. 2003, Japanese, 化学工学会, 東京, Domestic conference分子進化法を用いたPLDの触媒機能変換Oral presentation
- 化学工学会第68年会, Mar. 2003, Japanese, 化学工学会, 東京, Domestic conference機能性DNA分子の探索と水質評価への応用Oral presentation
- YABEC2002, Nov. 2002, English, YABEC, 台北, International conferenceBio-sensing of benzene derivatives using Ps promoter gene and GFP as a reporter protein (II)Oral presentation
- 日本生物工学会第54回大会, Oct. 2002, Japanese, 日本生物工学会, 大阪, Domestic conference有機溶媒感受性プロモーターを用いた水質評価法の構築Oral presentation
- 日本生物工学会第54回大会, Oct. 2002, Japanese, 日本生物工学会, 大阪, Domestic conferenceリン脂質代謝酵素ホスホリパーゼDの遺伝子工学的手法による触媒機能解析Oral presentation
- 化学工学会第35回秋季大会, Sep. 2002, Japanese, 化学工学会, 神戸, Domestic conference有機化合物認識遺伝子群を用いた水質評価法の構築Oral presentation
- 化学工学会第35回秋季大会, Sep. 2002, Japanese, 化学工学会, 神戸, Domestic conference変異型ホスホリパーゼDを用いた新規リン脂質合成への検討Oral presentation
- 化学工学会第35回秋季大会, Sep. 2002, Japanese, 化学工学会, 神戸, Domestic conference二酸化チタン界面での超音波力学的殺菌メカニズムの解析Oral presentation
- 化学工学会第35回秋季大会, Sep. 2002, Japanese, 化学工学会, 神戸, Domestic conference二酸化チタン・超音波法による内分泌撹乱物質の分解処理に関する研究Oral presentation
- 化学工学会第67年会, Mar. 2002, Japanese, 化学工学会, 福岡, Domestic conference有機溶媒感受性プロモーターを用いた水質評価法の構築Oral presentation
- 化学工学会第67年会, Mar. 2002, Japanese, 化学工学会, 福岡, Domestic conference二酸化チタン超音波照射法によるヒドロキシラジカルの生成と内分泌撹乱物質の分解処理Oral presentation
- 化学工学会第67年会, Mar. 2002, Japanese, 化学工学会, 福岡, Domestic conferenceホスホリパーゼDにおける構造変化とリン脂質合成相関に関する解析Oral presentation
- YABEC2001, Oct. 2001, English, YABEC, 上海, International conferenceConstruction of functional phospholipid synthesis system by using mutant phospholipase D enzymeOral presentation
- YABEC2001, Oct. 2001, English, YABEC, 上海, International conferenceConstruction of benzene derivatives biosensor using Ps promoter gene and GFP as a reporter geneOral presentation
- 化学工学会福井大会, Jul. 2001, Japanese, 化学工学会, 福井, Domestic conferenceホスホリパーゼDの触媒機構解析と新規リン脂質合成への応用Oral presentation
- 化学工学会第66年会, Apr. 2001, Japanese, 化学工学会, 東広島, Domestic conferenceレポーター遺伝子組換え微生物を用いる水質評価法の構築Oral presentation
- 化学工学会第66年会, Apr. 2001, Japanese, 化学工学会, 東広島, Domestic conferenceリン脂質代謝酵素ホスホリパーゼD の触媒機構及び活性化機構の解析Oral presentation
- YABEC2000, Nov. 2000, English, YABEC, 福岡, International conferenceImproved catalytic properties of phospholipase D by site directed mutagenesisOral presentation
- 化学工学会秋季大会, Sep. 2000, English, 化学工学会, 浜松, Domestic conferenceSecretory Overexpression of Phospholipase D By Recombinant Streptomyces lividansOral presentation
- 日本生物工学会第52回大会, Aug. 2000, Japanese, 日本生物工学会, 札幌, Domestic conference遺伝子工学的手法による活性変異型ホスホリパーゼDの構築と解析Oral presentation
- 日本生物工学会第51回大会, Sep. 1999, Japanese, 日本生物工学会, 吹田市, Domestic conference放線菌Stv. cinnamoneum由来PLDの発現系構築と触媒機構解析Oral presentation
- 学術研究助成基金助成金/基盤研究(C), Apr. 2019 - Mar. 2022過酸化チタンナノ粒子を用いたTheranostic粒子線治療の開発Competitive research funding
- 学術研究助成基金助成金/基盤研究(C), Apr. 2017 - Mar. 2020Competitive research funding
- 科学研究費補助金/基盤研究(B), Apr. 2016 - Mar. 2019, Principal investigatorCompetitive research funding
- 学術研究助成基金助成金/挑戦的萌芽研究, Apr. 2016 - Mar. 2019Competitive research funding
- 国立研究開発法人科学技術振興機構, 国際科学技術共同研究推進事業・地球規模課題対応国際科学技術協力プログラム(SATREPSインドネシア), Nov. 2017 - Mar. 2018, Principal investigator【JST-SATREPS】インドネシアにおける統合バイオリファイナリーシステムの開発Competitive research funding
- 国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業(CREST), 2013 - 2018, Principal investigator【CREST】植物バイオマスを原料とした芳香族ポリマー原料の発酵生産Competitive research funding
- 国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業(先端的低炭素化技術開発ALCA), 2017, Principal investigator【ALCA】イオン液体前処理バイオマスの酵素糖化、および得られる糖液の特性評価Competitive research funding
- 学術研究助成基金助成金/基盤研究(C), Apr. 2013 - Mar. 2016, Principal investigatorCompetitive research funding
- 学術研究助成基金助成金/挑戦的萌芽研究, Apr. 2013 - Mar. 2016Competitive research funding
- 戦略的イノベーション創造プログラム(SIP), 2016, Principal investigator酵素法による植物油からのBDFと副産物バイオマスへの変換(基礎条件検討)Competitive research funding
- 国際科学技術共同研究推進事業・地球規模課題対応国際科学技術協力プログラム(SATREPSインドネシア), 2016, Principal investigator【JST-SATREPS】インドネシアにおける統合バイオリファイナリーシステムの開発Competitive research funding
- 国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業(CREST), 2016, Principal investigator【CREST】植物バイオマスを原料とした芳香族ポリマー原料の発酵生産Competitive research funding
- 国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業(ALCA), 2016, Principal investigator【ALCA】イオン液体前処理バイオマスの酵素糖化、および得られる糖液の特性評価Competitive research funding
- 科学研究費補助金/基盤研究(B), Apr. 2012 - Mar. 2015Competitive research funding
- 科学研究費補助金/基盤研究(B), Apr. 2012 - Mar. 2015Competitive research funding
- 地球規模課題対応国際科学技術協力プログラム, 2015, Principal investigatorSATREPS「インドネシアにおける統合バイオリファイナリーシステムの開発」Competitive research funding
- 地球規模課題対応国際科学技術協力プログラム, 2014, Principal investigatorSATREPS「インドネシアにおける統合バイオリファイナリーシステムの開発」Competitive research funding
- 二国間交流事業(インドネシアとの共同研究), 2013, Principal investigator二国間交流「酵母細胞表層工学によるエタノール・マンノオリゴ糖生産の統合バイオプロセス開発」Competitive research funding
- 地球規模課題対応国際科学技術協力プログラム, 2013, Principal investigatorSATREPS「統合バイオリファイナリー研究拠点構築プロジェクト(インドネシア)」Competitive research funding
- 二国間交流事業(インドネシアとの共同研究), 2012, Principal investigator二国間交流「酵母細胞表層工学によるエタノール・マンノオリゴ糖生産の統合バイオプロセス開発」Competitive research funding
- 独立行政法人新エネルギー・産業技術総合開発機構, バイオマスエネルギー等高効率転換技術開発, 2012, Principal investigatorイオン液体を利用したバイオマスからのバイオ燃料生産技術の開発Competitive research funding
- 地球規模課題対応国際科学技術協力プログラム, 2012, Principal investigatorSATREPS「インドネシアにおける統合バイオリファイナリーシステムの開発」Competitive research funding
- 二国間交流事業(インドネシアとの共同研究), 2011, Principal investigator二国間交流「酵母細胞表層工学によるエタノール・マンノオリゴ糖生産の統合バイオプロセス開発」Competitive research funding
- 学術研究助成基金助成金/挑戦的萌芽研究, 2011Competitive research funding
- 独立行政法人新エネルギー・産業技術総合開発機構, バイオマスエネルギー等高効率転換技術開発, 2011, Principal investigatorイオン液体を利用したバイオマスからのバイオ燃料生産技術の開発Competitive research funding
- 2010, Principal investigator産業技術研究「高分解能生体分子プローブカンチレバーの創製による生体認識イメージング技術の開発」Competitive research funding
- 科学研究費補助金/若手研究(B), 2009, Principal investigatorCompetitive research funding
- 2009, Principal investigator産業技術研究「高分解能生体分子プローブカンチレバーの創製による生体認識イメージング技術の開発」Competitive research funding
- 2008, Principal investigator産業技術研究「高分解能生体分子プローブカンチレバーの創製による生体認識イメージング技術の開発」(2)Competitive research funding
- 2008, Principal investigator産業技術研究「高分解能生体分子プローブカンチレバーの創製による生体認識イメージング技術の開発」(1)Competitive research funding
- 科学研究費補助金/若手研究(B), 2007, Principal investigatorCompetitive research funding
- 2007, Principal investigator放射菌によるP450系酵素群の大量発現系の開発Competitive research funding
- 科学研究費補助金/特定領域研究, 2007, Principal investigatorCompetitive research funding
- 科学研究費補助金/特定領域研究, 2007, Principal investigatorCompetitive research funding
- 糖液の製造方法及び多糖類系バイオマス由来化合物の製造方法特願2014-129051, 24 Jun. 2014, 大学長, 特許6403188, 21 Sep. 2018Patent right
- βグルコシダーゼを発現する大腸菌特願2010-258361, 18 Nov. 2010, 大学長, 特許5846523, 04 Dec. 2015Patent right
- 放射線治療剤特願2012-500638, 17 Feb. 2011, 大学長, 特許5649137, 21 Nov. 2014Patent right
- 放射線治療剤(中)201180008541.3, 17 Feb. 2011, 大学長, ZL201180008541.3, 02 Jul. 2014Patent right
- 放射線治療剤13/578836, 17 Feb. 2011, 大学長, 8580312, 12 Nov. 2013Patent right
- セルロース含有材料からの有用物質の生産方法特願2009-183880, 06 Aug. 2009, 大学長, 特許5120965, 02 Nov. 2012Patent right