Research activity information

• Mar. 2023 Phytopathological Society of Japan, Academic award, いもち病菌のエピジェネティクスと病原性に関する研究

  Hitoshi Nakayashiki


• Sep. 2016 講談社, 講談社科学出版賞, ウイルスは生きている

  NAKAYASHIKI HITOSHI

  Publisher


• Aug. 2015 日本植物病理学会, 植物感染生理談話会ポスター発表優秀賞, Ralstonia solanacearum OE1-1株のバイオフィルム形成にレクチンES-IILは必要である

  森 友花, 井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 大西 秀幸, 甲斐 建次, 木場 章範, 大西 浩平, 曳地 康史

  Japan society


• 2011 日本植物病理学会, 日本植物病理学会論文賞, Chuma, I., Shinogi, T., Hosogi, N., Ikeda, K., Nakayashiki, H., Park, P., and Tosa, Y. (2009) Cytological characteristics of microconidia of Magnaporthe oryzae. J. Gen. Plant Pathol. 75：353-358

  Chuma Izumi, Shinogi, T, Hosogi, N, Ikeda Kenichi, Nakayashiki Hitoshi, Park Pyoyun, TOSA YUKIO

  Official journal

• Asynchronous evolution of centromeric sequences across chromosomes in Pyricularia oryzae.

  Atsumi Morimoto, Thach An Dang, Ken-Ichi Ikeda, Hitoshi Nakayashiki

  Centromeres are essential for chromosome segregation, yet they are among the most rapidly evolving regions of the genome. The mechanisms driving this rapid evolution of centromeric sequences are still not well understood. In this study, we identified the centromeric sequences of a wheat-infecting Pyricularia oryzae strain (Br48) using CENP-A chromatin immunoprecipitation followed by high-throughput sequencing. The Br48 centromeres range from 71 kb to 101 kb in length and are highly AT-rich (72.1-75.5%) and repeat-rich (63.4-85.0%). These regions are also enriched in H3K9me3 and 5-methylcytosine but depleted of H3K4me2 and H3K27me3. During the analysis of repetitive sequences in the Br48 centromere, we identified a stretch of approximately 530 bp that is tightly associated with centromeres in the Pyricularia fungus. We designated this element as the centromere-associated IR element (CenIR), as it often forms inverted repeat structures with two elements adjacent in reverse orientation. A comparison of putative centromere sequences across phylogenetically distinct P. oryzae strains suggests that changes in centromeric sequences are non-uniform across chromosomes and do not always align with the fungal phylogenetic relationships. Repeat-induced point mutation (RIP)-like CG to TA transitions likely accelerate base substitutions in the centromeres of Pyricularia fungi.

  Jan. 2025, Genes & genetic systems, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The secret life of the Pyricularia fungi in the rhizosphere

  Zikai Xiang, Hitoshi Nakayashiki, Kenichi Ikeda

  Elsevier BV, Dec. 2024, Fungal Biology, 101524 - 101524

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The ACE1 secondary metabolite gene cluster is a pathogenicity factor of wheat blast fungus.

  Trinh T P Vy, Yoshihiro Inoue, Soichiro Asuke, Izumi Chuma, Hitoshi Nakayashiki, Yukio Tosa

  Wheat blast caused by Pyricularia oryzae pathotype Triticum is now becoming a very serious threat to global food security. Here, we report an essential pathogenicity factor of the wheat blast fungus that is recognized and may be targeted by a rice resistance gene. Map-based cloning of Pwt2 showed that its functional allele is the ACE1 secondary metabolite gene cluster of the wheat blast fungus required for its efficient penetration of wheat cell walls. ACE1 is required for the strong aggressiveness of Triticum, Eleusine, and Lolium pathotypes on their respective hosts, but not for that of Oryza and Setaria pathotypes on rice and foxtail millet, respectively. All ACE1 alleles found in wheat blast population are recognized by a rice resistance gene, Pi33, when introduced into rice blast isolates. ACE1 mutations for evading the recognition by Pi33 do not affect the aggressiveness of the rice blast fungus on rice but inevitably impair the aggressiveness of the wheat blast fungus on wheat. These results suggest that a blast resistance gene already defeated in rice may be revived as a durable resistance gene in wheat by targeting an Achilles heel of the wheat blast fungus.

  Jul. 2024, Communications biology, 7(1) (1), 812 - 812, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Specific detection of Pyricularia oryzae pathotype Triticum using qPCR and LAMP methods

  Ken-ichi Ikeda, Kaichi Uchihashi, Itsuki Okuda, Zikai Xiang, Hitoshi Nakayashiki

  Abstract Wheat blast is an important disease that affects cross-border food security. The causal pathogen, Pyricularia oryzae (syn. Magnaporthe oryzae) Triticum pathotype (MoT), forms a phylogenetic group distinct from the other P. oryzae pathotypes pathogenic on various gramineous plants. Here we developed MoT-specific primer sets for qPCR and LAMP detection methods, and their amplification specificity and efficiency were tested in comparison with previous reported MoT-specific primers. The amplification efficiency and specificity were affected by the polymerase premix. To evaluate the detection specificity, purified DNA samples of 15 MoT isolates, 89 non-MoT isolates of Pyricularia spp., and crude DNA from wheat plants inoculated with MoT were used. We conclude that the Sp3 primer sets for qPCR and the MoT3L primer sets for LAMP were the best in this research.

  Last, Springer Science and Business Media LLC, Dec. 2023, Journal of General Plant Pathology

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Horizontally Transferred DNA in the Genome of the Fungus Pyricularia oryzae is Associated With Repressive Histone Modifications

  Natsuki Kobayashi, Thach An Dang, Kieu Thi Minh Pham, Luis B Gómez Luciano, Ba Van Vu, Kosuke Izumitsu, Motoki Shimizu, Ken-ichi Ikeda, Wen-Hsiung Li, Hitoshi Nakayashiki

  Abstract Horizontal gene transfer (HGT) is a means of exchanging genetic material asexually. The process by which horizontally transferred genes are domesticated by the host genome is of great interest but is not well understood. In this study, we determined the telomere-to-telomere genome sequence of the wheat-infecting Pyricularia oryzae strain Br48. SNP analysis indicated that the Br48 strain is a hybrid of wheat- and Brachiaria-infecting strains by a sexual or parasexual cross. Comparative genomic analysis identified several megabase-scale “insertions” in the Br48 genome, some of which were possibly gained by HGT-related events from related species, such as P. pennisetigena or P. grisea. Notably, the mega-insertions often contained genes whose phylogeny is not congruent with the species phylogeny. Moreover, some of the genes have a close homolog even in distantly related organisms, such as basidiomycetes or prokaryotes, implying the involvement of multiple HGT events. Interestingly, the levels of the silent epigenetic marks H3K9me3 and H3K27me3 in a genomic region tended to be negatively correlated with the phylogenetic concordance of genes in the same region, suggesting that horizontally transferred DNA is preferentially targeted for epigenetic silencing. Indeed, the putative HGT-derived genes were activated when MoKmt6, the gene responsible for H3K27me3 modification, was deleted. Notably, these genes also tended to be up-regulated during infection, suggesting that they are now under host control and have contributed to establishing a fungal niche. In conclusion, this study suggests that epigenetic modifications have played an important role in the domestication of HGT-derived genes in the P. oryzae genome.

  Oxford University Press (OUP), Aug. 2023, Molecular Biology and Evolution, 40(9) (9), English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Studies on the roles of epigenetics in the pathogenicity of Pyricularia oryzae

  Hitoshi Nakayashiki

  Springer Science and Business Media LLC, Aug. 2023, Journal of General Plant Pathology, 89(6) (6), 361 - 364, English

  [Refereed][Invited]

  Scientific journal


• MoSET1-dependent transcription factors regulate different stages of infection-related morphogenesis in Pyricularia oryzae

  Dang Ngoc Minh, Yusaku Tsukahara, Dang An Thach, Ken-ich Ikeda, Hitoshi Nakayashiki

  Springer Science and Business Media LLC, Dec. 2022, Journal of General Plant Pathology, 89(2) (2), 77 - 83, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Novel insights into host specificity of Pyricularia oryzae and Pyricularia grisea in the infection of gramineous plant roots

  Zikai Xiang, Daiki Okada, Soichiro Asuke, Hitoshi Nakayashiki, Kenichi Ikeda

  Wiley, Aug. 2022, Molecular Plant Pathology, 23(11) (11), 1658 - 1670

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Copy number-dependent DNA methylation of the Pyricularia oryzae MAGGY retrotransposon is triggered by DNA damage.

  Ba Van Vu, Quyet Nguyen, Yuki Kondo-Takeoka, Toshiki Murata, Naoki Kadotani, Giang Thi Nguyen, Takayuki Arazoe, Shuichi Ohsato, Hitoshi Nakayashiki

  Transposable elements are common targets for transcriptional and post-transcriptional gene silencing in eukaryotic genomes. However, the molecular mechanisms responsible for sensing such repeated sequences in the genome remain largely unknown. Here, we show that machinery of homologous recombination (HR) and RNA silencing play cooperative roles in copy number-dependent de novo DNA methylation of the retrotransposon MAGGY in the fungus Pyricularia oryzae. Genetic and physical interaction studies revealed that RecA domain-containing proteins, including P. oryzae homologs of Rad51, Rad55, and Rad57, together with an uncharacterized protein, Ddnm1, form complex(es) and mediate either the overall level or the copy number-dependence of de novo MAGGY DNA methylation, likely in conjunction with DNA repair. Interestingly, P. oryzae mutants of specific RNA silencing components (MoDCL1 and MoAGO2) were impaired in copy number-dependence of MAGGY methylation. Co-immunoprecipitation of MoAGO2 and HR components suggested a physical interaction between the HR and RNA silencing machinery in the process.

  Mar. 2021, Communications biology, 4(1) (1), 351 - 351, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Evolution of an Eleusine-Specific Subgroup of Pyricularia oryzae Through a Gain of an Avirulence Gene.

  Soichiro Asuke, Masaki Tanaka, Gang-Su Hyon, Yoshihiro Inoue, Trinh Thi Phuong Vy, Daisuke Niwamoto, Hitoshi Nakayashiki, Yukio Tosa

  Eleusine isolates (members of the Eleusine pathotype) of Pyricularia oryzae are divided into two subgroups, EC-I and EC-II, differentiated by molecular markers. A multilocus phylogenetic analysis revealed that these subgroups are very close to Eragrostis isolates. EC-II and Eragrostis isolates were exclusively virulent on finger millet and weeping lovegrass, respectively, while EC-I isolates were virulent on both. The avirulence of EC-II on weeping lovegrass was conditioned by an avirulence gene, PWL1. All EC-II isolates shared a peculiar structure (P structure) that was considered to be produced by an insertion (or translocation) of a DNA fragment carrying PWL1. On the other hand, all EC-I and Eragrostis isolates were noncarriers of PWL1 and shared a gene structure that should have predated the insertion of the PWL1-containing fragment. These results, together with phylogenetic analyses using whole-genome sequences, suggest that the Eleusine-specific subgroup (EC-II) evolved through a loss of pathogenicity on weeping lovegrass caused by a gain of PWL1.

  Feb. 2020, Molecular plant-microbe interactions : MPMI, 33(2) (2), 153 - 165, English, International magazine

  [Refereed]

  Scientific journal


• Transcriptome analysis of the fungal pathogen Rosellinia necatrix during infection of a susceptible avocado rootstock identifies potential mechanisms of pathogenesis.

  A Zumaquero, S Kanematsu, H Nakayashiki, A Matas, E Martínez-Ferri, A Barceló-Muñóz, F Pliego-Alfaro, C López-Herrera, F M Cazorla, C Pliego

  BACKGROUND: White root rot disease caused by Rosellinia necatrix is one of the most important threats affecting avocado productivity in tropical and subtropical climates. Control of this disease is complex and nowadays, lies in the use of physical and chemical methods, although none have proven to be fully effective. Detailed understanding of the molecular mechanisms underlying white root rot disease has the potential of aiding future developments in disease resistance and management. In this regard, this study used RNA-Seq technology to compare the transcriptomic profiles of R. necatrix during infection of susceptible avocado 'Dusa' roots with that obtained from the fungus cultured in rich medium. RESULTS: The transcriptomes from three biological replicates of R. necatrix colonizing avocado roots (RGA) and R. necatrix growing on potato dextrose agar media (RGPDA) were analyzed using Illumina sequencing. A total of 12,104 transcripts were obtained, among which 1937 were differentially expressed genes (DEG), 137 exclusively expressed in RGA and 160 in RGPDA. During the root infection process, genes involved in the production of fungal toxins, detoxification and transport of toxic compounds, hormone biosynthesis, gene silencing and plant cell wall degradation were overexpressed. Interestingly, 24 out of the 137 contigs expressed only during R. necatrix growth on avocado roots, were predicted as candidate effector proteins (CEP) with a probability above 60%. The PHI (Pathogen Host Interaction) database revealed that three of the R. necatrix CEP showed homology with previously annotated effectors, already proven experimentally via pathogen-host interaction. CONCLUSIONS: The analysis of the full-length transcriptome of R. necatrix during the infection process is suggesting that the success of this fungus to infect roots of diverse crops might be attributed to the production of different compounds which, singly or in combination, interfere with defense or signaling mechanisms shared among distinct plant families. The transcriptome analysis of R. necatrix during the infection process provides useful information and facilitates further research to a more in -depth understanding of the biology and virulence of this emergent pathogen. In turn, this will make possible to evolve novel strategies for white root rot management in avocado.

  Dec. 2019, BMC genomics, 20(1) (1), 1016 - 1016, English, International magazine

  [Refereed]

  Scientific journal


• Three ourmia-like viruses and their associated RNAs in Pyricularia oryzae.

  Shuhei Ohkita, Yui Lee, Quyet Nguyen, Kenichi Ikeda, Nobuhiro Suzuki, Hitoshi Nakayashiki

  Three ourmia-like viruses, designated Pyricularia oryzae ourmia-like virus (PoOLV) 1 to 3, were identified in a wheat-infecting isolate of P. oryzae. The sizes of the full-length PoOLV1-3 genomes were determined to be 2,528, 1,671, and 2,557 nt. Interestingly, we also found two abundant single-stranded RNAs sharing their 5' terminal 25 and 255 nt with PoOLV1 RNA and PoOLV3 RNA, respectively. The PoOLV1- and PoOLV3-associated RNAs (ARNA1 and ARNA3) were 639 and 514 nt in length, and possessed one and two small ORFs, respectively. In the field isolates of P. oryzae, PoOLVs and ARNAs were detectable at varying levels, and the levels of PoOLV1 and ARNA1 as well as those of PoOLV3 and ARNA3, were tightly correlated. In addition, gene silencing of PoOLV1 and PoOLV3 resulted in a reduction of ARNA1 and ARNA3, respectively. There results indicated that replication of ARNA1 and ARNA3 was associated with PoOLV1 and PoOLV3, respectively.

  Aug. 2019, Virology, 534, 25 - 35, English, International magazine

  [Refereed]

  Scientific journal


• Blast Fungal Genomes Show Frequent Chromosomal Changes, Gene Gains and Losses, and Effector Gene Turnover.

  Luis B Gómez Luciano, Isheng Jason Tsai, Izumi Chuma, Yukio Tosa, Yi-Hua Chen, Jeng-Yi Li, Meng-Yun Li, Mei-Yeh Jade Lu, Hitoshi Nakayashiki, Wen-Hsiung Li

  Pyricularia is a fungal genus comprising several pathogenic species causing the blast disease in monocots. Pyricularia oryzae, the best-known species, infects rice, wheat, finger millet, and other crops. As past comparative and population genomics studies mainly focused on isolates of P. oryzae, the genomes of the other Pyricularia species have not been well explored. In this study, we obtained a chromosomal-level genome assembly of the finger millet isolate P. oryzae MZ5-1-6 and also highly contiguous assemblies of Pyricularia sp. LS, P. grisea, and P. pennisetigena. The differences in the genomic content of repetitive DNA sequences could largely explain the variation in genome size among these new genomes. Moreover, we found extensive gene gains and losses and structural changes among Pyricularia genomes, including a large interchromosomal translocation. We searched for homologs of known blast effectors across fungal taxa and found that most avirulence effectors are specific to Pyricularia, whereas many other effectors share homologs with distant fungal taxa. In particular, we discovered a novel effector family with metalloprotease activity, distinct from the well-known AVR-Pita family. We predicted 751 gene families containing putative effectors in 7 Pyricularia genomes and found that 60 of them showed differential expression in the P. oryzae MZ5-1-6 transcriptomes obtained under experimental conditions mimicking the pathogen infection process. In summary, this study increased our understanding of the structural, functional, and evolutionary genomics of the blast pathogen and identified new potential effector genes, providing useful data for developing crops with durable resistance.

  Corresponding, Oxford press, Jun. 2019, Molecular biology and evolution, 36(6) (6), 1148 - 1161, English, International magazine

  [Refereed]

  Scientific journal


• Ralfuranones contribute to mushroom-type biofilm formation by Ralstonia solanacearum strain OE1-1

  Yuka Mori, Yuki Hosoi, Shiho Ishikawa, Kazusa Hayashi, Yu Asai, Hideyuki Ohnishi, Mika Shimatani, Kanako Inoue, Kenichi Ikeda, Hitoshi Nakayashiki, Yasuyo Nishimura, Kouhei Ohnishi, Akinori Kiba, Kenji Kai, Yasufumi Hikichi

  Apr. 2018, Molecular Plant Pathology, 19(4) (4), 975 - 985, English

  [Refereed]

  Scientific journal


• Erratum: A fungal Argonaute interferes with RNA interference (Nucleic acids research (2018) 46 5 (2495-2508))

  Quyet Nguyen, Akihide Iritani, Shuhei Ohkita, Ba V. Vu, Kana Yokoya, Ai Matsubara, Ken-Ichi Ikeda, Nobuhiro Suzuki, Hitoshi Nakayashiki

  NLM (Medline), Mar. 2018, Nucleic acids research, 46(5) (5), 2698, English

  Scientific journal


• A fungal Argonaute interferes with RNA interference.

  Quyet Nguyen, Akihide Iritani, Shuhei Ohkita, Ba V Vu, Kana Yokoya, Ai Matsubara, Ken-Ichi Ikeda, Nobuhiro Suzuki, Hitoshi Nakayashiki

  Small RNA (sRNA)-mediated gene silencing phenomena, exemplified by RNA interference (RNAi), require a unique class of proteins called Argonautes (AGOs). An AGO protein typically forms a protein-sRNA complex that contributes to gene silencing using the loaded sRNA as a specificity determinant. Here, we show that MoAGO2, one of the three AGO genes in the fungus Pyricularia oryzae (Magnaporthe oryzae) interferes with RNAi. Gene knockout (KO) studies revealed that MoAGO1 and MoAGO3 additively or redundantly played roles in hairpin RNA- and retrotransposon (MAGGY)-triggered RNAi while, surprisingly, the KO mutants of MoAGO2 (Δmoago2) showed elevated levels of gene silencing. Consistently, transcript levels of MAGGY and mycoviruses were drastically reduced in Δmoago2, supporting the idea that MoAGO2 impeded RNAi against the parasitic elements. Deep sequencing analysis revealed that repeat- and mycovirus-derived small interfering RNAs were mainly associated with MoAGO2 and MoAGO3, and their populations were very similar based on their size distribution patterns and positional base preference. Site-directed mutagenesis studies indicated that sRNA binding but not slicer activity of MoAGO2 was essential for the ability to diminish the efficacy of RNAi. Overall, these results suggest a possible interplay between distinct sRNA-mediated gene regulation pathways through a competition for sRNA.

  Mar. 2018, Nucleic acids research, 46(5) (5), 2495 - 2508, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Acidic soil conditions suppress zoospore release from zoosporangia in Olpidium virulentus

  Yutaka Iwamoto, Kai Inoue, Shinji Nishiguchi, Katsunari Matsuura, Masataka Aino, Hitoshi Nakayashiki, Kenichi Ikeda

  Jul. 2017, JOURNAL OF GENERAL PLANT PATHOLOGY, 83(4) (4), 240 - 243, English

  [Refereed]

  Scientific journal


• pH降下型肥料の施用がOlpidium virulentusの感染とレタスビックベイン病の発生に及ぼす影響

  岩本 豊, 井上 海, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 田中 正, 佐藤 博友, 西口 真嗣, 松浦 克成, 中野 伸一

  We conducted laboratory experiments to assess the disease control effects of pH-decreasing fertilizer as a new control material for lettuce big-vein disease. Results show that the occurrence of big-vein disease was suppressed in concomitance with the descent of soil pH by applying the fertilizer.

  The Kansai Plant Protection Society, May 2017, 関西病虫害研究会報, 59(59) (59), 51 - 53, Japanese

  [Refereed]

  Scientific journal


• The vascular plant-pathogenic bacterium Ralstonia solanacearum produces biofilms required for its virulence on the surfaces of tomato cells adjacent to intercellular spaces

  Yuka Mori, Kanako Inoue, Kenichi Ikeda, Hitoshi Nakayashiki, Chikaki Higashimoto, Kouhei Ohnishi, Akinori Kiba, Yasufumi Hikichi

  Aug. 2016, MOLECULAR PLANT PATHOLOGY, 17(6) (6), 890 - 902, English

  [Refereed]

  Scientific journal


• 糸状菌病害の組織特異的感染戦略 －いもち病菌の生い立ちを紐解く－

  IKEDA KENICHI, 井上 加奈子, 森田 雄一, 伊藤 里紗, 中本 知里, 植 宏美, 翠川 陽大, 藤原 有紗, Talbot J Nicholas, NAKAYASHIKI HITOSHI

  日本植物病理学会, 2016, 植物感染生理談話会論文集, (51) (51), 83 - 94, Japanese

  [Invited]

  Symposium


• Field method to monitor the mycoparasitic fungus Coniothyrium minitans

  Ryo Shukunami, Yutaka Iwamoto, Shinji Sugiura, Kentaro Ikeda, Hitohsi Nakayashiki, Kenichi Ikeda

  Jan. 2016, JOURNAL OF GENERAL PLANT PATHOLOGY, 82(1) (1), 51 - 56, English

  [Refereed]

  Scientific journal


• Self- and nonself recognition during hyphal interactions in Rosellinia necatrix

  Takahiro Uwamori, Kanako Inoue, Chiaki Kida, Yuichi Morita, Pyoyun Park, Hitoshi Nakayashiki, Satoko Kanematsu, Kenichi Ikeda

  Nov. 2015, JOURNAL OF GENERAL PLANT PATHOLOGY, 81(6) (6), 420 - 428, English

  [Refereed]

  Scientific journal


• Mechanisms of biofilm formation by Ralstonia solanacearum and its involvement in the bacterial virulence

  曳地 康史, 森 友花, 石川 詩歩, 東本 周樹, 井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 木場 章範, 大西 浩平, 甲斐 建次

  Phytopathological Society of Japan, Sep. 2015, PSJ Plant-Microbe Interactions Symposium Report, (50) (50), 11 - 21, Japanese

  [Invited]

  Symposium


• Epigenetic gene regulation in the rice blast fungus Pyricularia oryzae

  Pham Thi Minh Kieu, 井上 義博, Vu Van Ba, IKEDA KENICHI, NAKAYASHIKI HITOSHI

  Phytopathological Society of Japan, Sep. 2015, PSJ Plant-Microbe Interactions Symposium Report, (50) (50), 113 - 121, Japanese

  [Invited]

  Symposium


• MoSET1 (Histone H3K4 Methyltransferase in Magnaporthe oryzae) Regulates Global Gene Expression during Infection-Related Morphogenesis

  Kieu Thi Minh Pham, Yoshihiro Inoue, Ba Van Vu, Hanh Hieu Nguyen, Toru Nakayashiki, Ken-ichi Ikeda, Hitoshi Nakayashiki

  Jul. 2015, PLOS GENETICS, 11(7) (7), e1005385, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Histone H3K4 methyltransferase globally regulates substrate-dependent activation of cell-wall-degrading enzymes in Magnaporthe oryzae

  Kieu Thi Minh Pham, Hanh Hieu Nguyen, Toshiko Murai, Izumi Chuma, Yukio Tosa, Hitoshi Nakayashiki

  Mar. 2015, JOURNAL OF GENERAL PLANT PATHOLOGY, 81(2) (2), 127 - 130, English

  [Refereed]

  Scientific journal


• 糸状菌の細胞質不和合性反応におけるプログラム細胞死機構の解析(2013年度大会一般講演要旨)

  上森 喬大, 井上 加奈子, 北澤 大知, 木田 千晶, 中屋敷 均, 兼松 聡子, 池田 健一

  日本土壌微生物学会, 2014, 土と微生物, 68(1) (1), 50 - 50, Japanese

  [Refereed]


• Substrate-Induced Transcriptional Activation of the MoCel7C Cellulase Gene Is Associated with Methylation of Histone H3 at Lysine 4 in the Rice Blast Fungus Magnaporthe oryzae

  Ba Van Vu, Kieu Thi Minh Pham, Hitoshi Nakayashiki

  Nov. 2013, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79(21) (21), 6823 - 6832, English

  [Refereed]

  Scientific journal


• Is the Fungus Magnaporthe Losing DNA Methylation?

  Ken-ichi Ikeda, Ba Van Vu, Naoki Kadotani, Masaki Tanaka, Toshiki Murata, Kohta Shiina, Izumi Chuma, Yukio Tosa, Hitoshi Nakayashiki

  Nov. 2013, GENETICS, 195(3) (3), 845 - +, English

  [Refereed]

  Scientific journal


• Appressorium-localized NADPH oxidase B is essential for aggressiveness and pathogenicity in the host-specific, toxin-producing fungus Alternaria alternataJapanese pear pathotype

  Yuichi Morita, Gang-Su Hyon, Naoki Hosogi, Nao Miyata, Hitoshi Nakayashiki, Yoshinori Muranaka, Noriko Inada, Pyoyun Park, Kenichi Ikeda

  May 2013, MOLECULAR PLANT PATHOLOGY, 14(4) (4), 365 - 378, English

  [Refereed]

  Scientific journal


• Molecular cloning of the apoptosis-related calcium-binding protein AsALG-2 in Avena sativa

  Trinh Xuan Hoat, Hitoshi Nakayashiki, Qian Yang, Yukio Tosa, Shigeyuki Mayama

  Apr. 2013, MOLECULAR PLANT PATHOLOGY, 14(3) (3), 222 - 229, English

  [Refereed]

  Scientific journal


• Cellulases Belonging to Glycoside Hydrolase Families 6 and 7 Contribute to the Virulence of Magnaporthe oryzae

  Ba Van Vu, Kenji Itoh, Quoc Bao Nguyen, Yukio Tose, Hitoshi Nakayashiki

  Sep. 2012, MOLECULAR PLANT-MICROBE INTERACTIONS, 25(9) (9), 1135 - 1141, English

  [Refereed]

  Scientific journal


• Implication of an Aldehyde Dehydrogenase Gene and a Phosphinothricin N-Acetyltransferase Gene in the Diversity of Pseudomonas cichorii Virulence

  Masayuki Tanaka, Ullah Md Wali, Hitoshi Nakayashiki, Tatsuya Fukuda, Hiroyuki Mizumoto, Kouhei Ohnishi, Akinori Kiba, Yasufumi Hikichi

  Mar. 2012, GENES, 3(1) (1), 62 - 80, English

  [Refereed]

  Scientific journal


• Systemic plant disease resistance induced by Pseudomonas fluorescens FPT9601

  Higashiyama Miyako, Ohara Yumiko, Kido Gou, Nishi Yuriko, Sakai Reona, Aino Kimitaka, Nakayashiki Hitoshi

  Pseudomonas fluorescens FPT9601株は,トマトにおいて植物成長促進根圏細菌として働き,ナス科植物青枯れ病菌に対する抵抗性を付与することで単離同定された。本菌はモデル植物であるシロイヌナズナにおいても,誘導全身抵抗性を引き起こす。シロイヌナズナのサリチル酸(SA),ジャスモン酸(JA)及びエチレン(ET)経路の変異株を用いて調査を行った所,FPT9601株が誘導する病害抵抗性にJAおよびET経路の関与が示唆された。次に約23.000遺伝子のプローブセットによるマイクロアレイ解析を行った結果,2倍以上の発現変動で上昇した遺伝子が95個,低下したものが105個であった。その中にオーキシン応答性遺伝子の発現増高が認められ,本菌による植物の成長促進はオーキシンの作用であることが示唆された。この他に二次代謝経路に関わる遺伝子の誘導や抑制,タンパク質合成系遺伝子の抑制などの傾向が認められた。また根粒菌感染で発現が増高することから命名されたノジュリン遺伝子群に含まれる遺伝子の誘導なども見られた。マイクロアレイ解析の結果,FPT9601感染により発現が上昇していた遺伝子のトランスポゾン挿入変異体におけるFPT9601株による誘導全身抵抗性について調査した所,転写因子などいくつかの変異体において,誘導抵抗性の低下が認められた。

  Japanese Society of Soil Microbiology, 2012, Soil Microorganisms, 66(2) (2), 43 - 46, Japanese


• In situ localization of avenanthramide A and its biosynthetic enzyme in oat leaves infected with the crown rust fungus, Puccinia coronata f. sp avenae

  Kohhei Uchihashi, Hitoshi Nakayashiki, Kaoru Okamura, Atsushi Ishihara, Yukio Tosa, Pyoyun Park, Shigeyuki Mayama

  Dec. 2011, PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 76(3-4) (3-4), 173 - 181, English

  [Refereed]

  Scientific journal


• Simultaneous silencing of endo-beta-1,4 xylanase genes reveals their roles in the virulence of Magnaporthe oryzae

  Quoc Bao Nguyen, Kenji Itoh, Ba Van Vu, Yukio Tosa, Hitoshi Nakayashiki

  Aug. 2011, MOLECULAR MICROBIOLOGY, 81(4) (4), 1008 - 1019, English

  [Refereed]

  Scientific journal


• Multiple Translocation of the AVR-Pita Effector Gene among Chromosomes of the Rice Blast Fungus Magnaporthe oryzae and Related Species

  Izumi Chuma, Chihiro Isobe, Yuma Hotta, Kana Ibaragi, Natsuru Futamata, Motoaki Kusaba, Kentaro Yoshida, Ryohei Terauchi, Yoshikatsu Fujita, Hitoshi Nakayashiki, Barbara Valent, Yukio Tosa

  Jul. 2011, PLOS PATHOGENS, 7(7) (7), e1002147, English

  [Refereed]

  Scientific journal


• Novel vectors for retrotransposon-induced gene silencing in Magnaporthe oryzae

  Ba Van Vu, Masako Takino, Toshiki Murata, Hitoshi Nakayashiki

  May 2011, JOURNAL OF GENERAL PLANT PATHOLOGY, 77(3) (3), 147 - 151, English

  [Refereed]

  Scientific journal


• Induction of Chlorosis, ROS Generation and Cell Death by a Toxin Isolated from Pyricularia oryzae

  Tetsu Tsurushima, Yukari Minami, Hisashi Miyagawa, Hitoshi Nakayashiki, Yukio Tosa, Shigeyuki Mayama

  Nov. 2010, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 74(11) (11), 2220 - 2225, English

  [Refereed]

  Scientific journal


• Methods to Study PAMP-Triggered Immunity Using Tomato and Nicotiana benthamiana

  Hanh P. Nguyen, Suma Chakravarthy, Andre C. Velasquez, Heather L. McLane, Lirong Zeng, Hitoshi Nakayashiki, Duck-Hwan Park, Alan Collmer, Gregory B. Martin

  Aug. 2010, MOLECULAR PLANT-MICROBE INTERACTIONS, 23(8) (8), 991 - 999, English

  [Refereed]

  Scientific journal


• Evolution of the Eleusine Subgroup of Pyricularia oryzae Inferred from Rearrangement at the Pwl1 Locus

  Masaki Tanaka, Gang-Su Hyon, Toshiki Murata, Hitoshi Nakayashiki, Yukio Tosa

  Jun. 2010, MOLECULAR PLANT-MICROBE INTERACTIONS, 23(6) (6), 771 - 783, English

  [Refereed]

  Scientific journal


• Pathological roles of reactive oxygen species in plant pathogen

  Hyon GS, Morita Y, Kusaka M, Hosogi N, IKEDA KENICHI, Nakayashiki H, Park P

  2010, Journal of Electron Microscopy Technology for Medicine and Biology, 23, 86, English

  International conference proceedings


• Cytological characteristics of microconidia of Magnaporthe oryzae

  Izumi Chuma, Takeshi Shinogi, Naoki Hosogi, Ken-ichi Ikeda, Hitoshi Nakayashiki, Pyoyun Park, Yukio Tosa

  Oct. 2009, JOURNAL OF GENERAL PLANT PATHOLOGY, 75(5) (5), 353 - 358, English

  [Refereed]

  Scientific journal


• Phylogenetic analyses of plant-growth-promoting rhizobacteria isolated from tomato, lettuce, and Japanese pepper plants in Hyogo Prefecture, Japan

  Tomohiro Kumatani, Yukihiko Yoshimi, Hitoshi Nakayashiki, Masataka Aino

  Aug. 2009, JOURNAL OF GENERAL PLANT PATHOLOGY, 75(4) (4), 316 - 321, English

  [Refereed]

  Scientific journal


• Population structure of Eleusine isolates of Pyricularia oryzae and its evolutionary implications

  Masaki Tanaka, Hitoshi Nakayashiki, Yukio Tosa

  Jun. 2009, JOURNAL OF GENERAL PLANT PATHOLOGY, 75(3) (3), 173 - 180, English

  [Refereed]

  Scientific journal


• Pathogenicity-Related Compounds Produced by Blast Fungus

  Tetsu Tsurushima, Hitoshi Nakayashiki, Yukio Tosa, Shigeyuki Mayama

  2009, ADVANCES IN GENETICS, GENOMICS AND CONTROL OF RICE BLAST DISEASE, 247 - +, English

  [Refereed]

  International conference proceedings


• Induction of reactive oxygen generation and functional changes in mitochondria and their involvement in the development of bacterial rot in lettuce caused by Pseudomonas cichorii

  Akinori Kiba, Kyon Ye Lee, Kouhei Ohnishi, Pyoyun Park, Hitoshi Nakayashiki, Yukio Tosa, Shigeyuki Mayama, Yasufumi Hikichi

  Jan. 2009, PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 74(1) (1), 45 - 54, English

  [Refereed]

  Scientific journal


• Transcriptional Control and Protein Specialization Have Roles in the Functional Diversification of Two Dicer-Like Proteins in Magnaporthe oryzae

  Naoki Kadotani, Toshiki Murata, Nguyen Bao Quoc, Yusuke Adachi, Hitoshi Nakayashiki

  Oct. 2008, GENETICS, 180(2) (2), 1245 - 1249, English

  [Refereed]

  Scientific journal


• Systematic functional analysis of calcium-signalling proteins in the genome of the rice-blast fungus, Magnaporthe oryzae, using a high-throughput RNA-silencing system

  Quoc Bao Nguyen, Naoki Kadotani, Seiji Kasahara, Yukio Tosa, Shigeyuki Mayama, Hitoshi Nakayashiki

  Jun. 2008, MOLECULAR MICROBIOLOGY, 68(6) (6), 1348 - 1365, English

  [Refereed]

  Scientific journal


• Pathological roles of reactive oxygen species at penetration pegs of Alternaria Japanese pear pathotype during plant infection

  Hyon GS, Morita Y, Hosogi N, IKEDA KENICHI, Nakayashiki H, Park P

  2008, Korean Journal of Microscopy, 38, 1235 - 1236, English

  International conference proceedings


• siRNA-dependent and -independent post-transcriptional cosuppression of the LTR-retrotransposon MAGGY in the phytopathogenic fungus Magnaporthe oryzae

  Toshiki Murata, Naoki Kadotani, Miki Yamaguchi, Yukio Tosa, Shigeyuki Mayama, Hitoshi Nakayashiki

  Sep. 2007, NUCLEIC ACIDS RESEARCH, 35(18) (18), 5987 - 5994, English

  [Refereed]

  Scientific journal


• Speciation in Pyyicularia inferred from multilocus phylogenetic analysis

  Kenji Hirata, Motoaki Kusaba, Izumi Chuma, Jun Osue, Hitoshi Nakayashiki, Shigeyuki Mayama, Yukio Tosa

  Jul. 2007, MYCOLOGICAL RESEARCH, 111(Pt 7) (Pt 7), 799 - 808, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Rwt4, a wheat gene for resistance to Avena isolates of Magnaporthe oryzae, functions as a gene for resistance to Panicum isolates in Japan. J.

  Hau, V.T.B, Hirata, K, Murakami, J, Nakayashiki, H, Mayama, S, Tosa, Y

  2007, J. Gen. Plant Pathol., 73, 22 - 28

  [Refereed]


• Evolution and diversification of RNA silencing proteins in fungi

  Hitoshi Nakayashiki, Naoki Kadotani, Shigeyuki Mayama

  Jul. 2006, JOURNAL OF MOLECULAR EVOLUTION, 63(1) (1), 127 - 135, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Specific cleavage of ribosomal RNA and mRNA during victorin-induced apoptotic cell death in oat

  TX Hoat, H Nakayashiki, Y Tosa, S Mayama

  Jun. 2006, PLANT JOURNAL, 46(6) (6), 922 - 933, English

  [Refereed]

  Scientific journal


• Induction of apoptotic cell death leads to the development of bacterial rot caused by Pseudomonas cichorii

  A Kiba, Y Sangawa, K Ohnishi, N Yao, P Park, H Nakayashiki, Y Tosa, S Mayama, Y Hikichi

  Feb. 2006, MOLECULAR PLANT-MICROBE INTERACTIONS, 19(2) (2), 112 - 122, English

  [Refereed]

  Scientific journal


• Two phases of intracellular reactive oxygen species production during victorin-induced cell death in oats

  Masaru Sakamoto, Yasuomi Tada, Hitoshi Nakayashiki, Yukio Tosa, Shigeyuki Mayama

  Dec. 2005, Journal of General Plant Pathology, 71(6) (6), 387 - 394, English

  [Refereed]

  Scientific journal


• Pyrichalasin H production and pathogenicity of Digitaria-specific isolates of Pyricularia grisea

  T Tsurushima, LD Don, K Kawashima, J Murakami, H Nakayashiki, Y Tosa, S Mayama

  Nov. 2005, MOLECULAR PLANT PATHOLOGY, 6(6) (6), 605 - 613, English

  [Refereed]

  Scientific journal


• Evolution of an avirulence gene, AVR1-CO39, concomitant with the evolution and differentiation of Magnaporthe oryzae

  Y Tosa, J Osue, Y Eto, HS Oh, H Nakayashiki, S Mayama, SA Leong

  Nov. 2005, MOLECULAR PLANT-MICROBE INTERACTIONS, 18(11) (11), 1148 - 1160, English

  [Refereed]

  Scientific journal


• Significance of PWT4-Rwt4 interaction in the species specificity of Avena isolates of Magnaporthe oryzae on wheat

  Kenji Hirata, Yukio Tosa, Hitoshi Nakayashiki, Shigeyuki Mayama

  Oct. 2005, Journal of General Plant Pathology, 71(5) (5), 340 - 344, English

  [Refereed]

  Scientific journal


• RNA silencing as a tool for exploring gene function in ascomycete fungi

  H Nakayashiki, S Hanada, NB Quoc, N Kadotani, Y Tosa, S Mayama

  Apr. 2005, FUNGAL GENETICS AND BIOLOGY, 42(4) (4), 275 - 283, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The C-terminal chromodomain-like module in the integrase domain is crucial for high transposition efficiency of the retrotransposon MAGGY.

  NAKAYASHIKI Hitoshi, AWA T, TOSA Yukio, MAYAMA Shigeyuki

  2005, FEBS letters, 579,488-492, English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The C-terminal chromodomain-like module in the integrase domain is crucial for high transposition efficiency of the retrotransposon MAGGY

  H Nakayashiki, T Awa, Y Tosa, S Mayama

  Jan. 2005, FEBS LETTERS, 579(2) (2), 488 - 492, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• S. RNA silencing as a tool for exploring gene function in Ascomycete fungi.

  NAKAYASHIKI Hitoshi, HANADA S, QUOC N B, KADOTANI Naoki, TOSA Yukio, MAYAMA Shigeyuki

  2005, Fungal Genet. & Biol., 未記入, English

  Scientific journal


• RNA silencing in fungi: Mechanisms and applications.

  NAKAYASHIKI Hitoshi

  2005, FEBS Lett., 579 5950-5957, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• NO-overproducting transformants of Pseudomonas fluorescens with enhanced biocontrol abiloty against tomato bacterial wilt.

  WANG Y G, YANG Q, TOSA Yukio, NAKAYASHIKI Hitoshi, MAYAMA Shigeyuki

  2005, J. Gen. Plant. Pathol., 未記入, English

  Scientific journal


• NO-overproducing transformants of Pseudomonas fluorescens with enhanced biocontrol abiloty against tomato bacterial wilt.

  WANG Y.Q, YANG Q, TOSA Yukio, NAKAYASHIKI Hitoshi, MAYAMA Shigeyuki

  2005, J. Gen. Plant Pathol., 71 33-38, English

  [Refereed]

  Scientific journal


• Microarray analysis of gene expression profile induced by the endophytic plant growth promoting rhizobacteria, Pseudomonas fluorescens FPT9601-T6 in Arabidopsis.

  WANG Y.Q, OHARA Y, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

  2005, Molecular Plant-Microbe Interactions, 18 385-396, 385 - 396, English

  [Refereed]

  Scientific journal


• Microarray analysis of gene expression profile induced by the endophytic plant growth promoting rhizobacteria, Pseudomonas fluorescens FPT9601-T5 in Arabidopsis.

  WANG Y G, OHARA Y, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

  2005, Mol. Plant-Microbe Interact., English

  Scientific journal


• Coordinate involvement of cysteine protease and nuclease in the executive phase of plant apoptosis

  K Kusaka, Y Tada, T Shigemi, M Sakamoto, H Nakayashiki, Y Tosa, S Mayama

  Dec. 2004, FEBS LETTERS, 578(3) (3), 363 - 367, English

  Scientific journal


• One of the two Dicer-like proteins in the filamentous fungi Magnaporthe oryzae genome is responsible for hairpin RNA-triggered RNA silencing and related small interfering RNA accumulation

  N Kadotani, H Nakayashiki, Y Tosa, S Mayama

  Oct. 2004, JOURNAL OF BIOLOGICAL CHEMISTRY, 279(43) (43), 44467 - 44474, English

  Scientific journal


• Genetic constitution and pathogenicity of Lolium isolates of Magnaporthe oryzae in comparison with host species-specific pathotypes of the blast fungus

  Y Tosa, K Hirata, H Tamba, S Nakagawa, Chuma, I, C Isobe, J Osue, AS Urashima, LD Don, M Kusaba, H Nakayashiki, A Tanaka, T Tani, N Mori, S Mayama

  May 2004, PHYTOPATHOLOGY, 94(5) (5), 454 - 462, English

  Scientific journal


• Nitric oxide and reactive oxygen species do not elicit hypersensitive cell death but induce apoptosis in the adjacent cells during the defense response of oat

  Y Tada, T Mori, T Shinogi, N Yao, S Takahashi, S Betsuyaku, M Sakamoto, P Park, H Nakayashiki, Y Tosa, S Mayama

  Mar. 2004, MOLECULAR PLANT-MICROBE INTERACTIONS, 17(3) (3), 245 - 253, English

  [Refereed]

  Scientific journal


• Repeat-induced point mutation (RIP) in Magnaporthe grisea: Evidence for the presence of sexual cycle in nature

  K Ikeda, H Nakayashiki, Y Tosa, S Mayama

  2004, RICE BLAST: INTERACTION WITH RICE AND CONTROL, 57 - 63, English

  [Refereed]

  International conference proceedings


• Genetic constitution and pathogenicity of Lolium isolates of Magnaporthe oryzae in comparison with host species-specific pathotypes of the blast fungus

  Y. Tosa, K. Hirata, H. Tamba, S. Nakagawa, I. Chuma, C. Isobe, J. Osue, A. S. Urashima, L. D. Don, M. Kusaba, H. Nakayashiki, A. Tanaka, T. Tani, N. Mori, S. Mayama

  American Phytopathological Society, 2004, Phytopathology, 94(5) (5), 454 - 462, English

  Scientific journal


• Analysis of the involvement of hydroxyanthranilate hydroxycinnamoyltransferase and caffeoyl-CoA 3-O-methyltransferase in phytoalexin biosynthesis in oat

  Qian Yang, Hoat Xuan Trinh, Satoshi Imai, Atsushi Ishihara, Liqun Zhang, Hitoshi Nakayashiki, Yukio Tosa, Shigeyuki Mayama

  American Phytopathological Society, 2004, Molecular Plant-Microbe Interactions, 17(1) (1), 81 - 89, English

  Scientific journal


• Nitric oxide and reactive oxygen species are not required for hypersensitive cell death but induce apoptosis in the adjacent cells during the defense response of oats.

  TADA Y, MORI T, SHINOGI T, YAO N, TAKAHASHI S, BETSUYAKU S, SAKAMOTO M, PARK P, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

  2004, Mol. Plant-Microbe Interact., 17,245-253, English

  Scientific journal


• Analysis of the involvement of hydroxyanthranilate hydroxycinnamoyltransferase and caffeoyl-CoA 3-O-methyltransferase in phytoalexin biosynthesis in oat

  Q Yang, HX Trinh, S Imai, A Ishihara, LQ Zhang, H Nakayashiki, Y Tosa, S Mayama

  Jan. 2004, MOLECULAR PLANT-MICROBE INTERACTIONS, 17(1) (1), 81 - 89, English

  Scientific journal


• RNA silencing in the phytopathogenic fungus Magnaporthe oryzae

  N Kadotani, H Nakayashiki, Y Tosa, S Mayama

  Sep. 2003, MOLECULAR PLANT-MICROBE INTERACTIONS, 16(9) (9), 769 - 776, English

  Scientific journal


• Meiotic behavior of a supernumerary chromosome in Magnaporthe oryzae

  Chuma, I, Y Tosa, M Taga, H Nakayashiki, S Mayama

  Jun. 2003, CURRENT GENETICS, 43(3) (3), 191 - 198, English

  Scientific journal


• Analysis of host species specificity of Magnaporthe grisea toward foxtail millet using a genetic cross between isolates from wheat and foxtail millet

  J Murakami, R Tomita, T Kataoka, H Nakayashiki, Y Tosa, S Mayama

  Jan. 2003, PHYTOPATHOLOGY, 93(1) (1), 42 - 45, English

  [Refereed]

  Scientific journal


• Nitric oxide and reactive oxygen species are not required for hypersensitive cell death but induce apoptosis in the adjacent cells during the defense response of oats.

  TADA Y, MORI T, SHINOGI T, YAO N, TAKAHASHI S, BETSUYAKU S, SAKAMOTO M, PARK P, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

  2003, Mol. Plant-MicrobeInteract., 未記入, English

  Scientific journal


• Characterization of an Avena isolate of Magnaporthe grisea and identification of a locus conditioning its specificity on oat

  HS Oh, Y Tosa, N Takabayashi, S Nakagawa, R Tomita, LD Don, M Kusaba, H Nakayashiki, S Mayama

  Oct. 2002, CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 80(10) (10), 1088 - 1095, English

  [Refereed]

  Scientific journal


• Apoptotic cell death is a common response to pathogen attack in oats

  N Yao, S Imai, Y Tada, H Nakayashiki, Y Tosa, P Park, S Mayama

  Oct. 2002, MOLECULAR PLANT-MICROBE INTERACTIONS, 15(10) (10), 1000 - 1007, English

  [Refereed]

  Scientific journal


• Repeat-induced point mutation (RIP) in Magnaporthe grisea: implications for its sexual cycle in the natural field context

  K Ikeda, H Nakayashiki, T Kataoka, H Tamba, Y Hashimoto, Y Tosa, S Mayama

  Sep. 2002, MOLECULAR MICROBIOLOGY, 45(5) (5), 1355 - 1364, English

  [Refereed]

  Scientific journal


• Mitochondrial oxidative burst involved in apoptotic response in oats

  N Yao, Y Tada, M Sakamoto, H Nakayashiki, P Park, Y Tosa, S Mayama

  Jun. 2002, PLANT JOURNAL, 30(5) (5), 567 - 579, English

  [Refereed]

  Scientific journal


• Current Status of Biological Control of Plant Diseases in China

  ZHANG Liqun, YAO Nan, WANG Ye, YANG Hetong, WANG Huimin, TANG Wenhua, TOSA Yukio, NAKAYASHIKI Hitoshi, MAYAMA Shigeyuki

  Kobe University, Feb. 2002, The science reports of Faculty of Agriculture, Kobe University, 26, 1 - 7, English


• Analysis of the structure of the AVR1-CO39 avirulence locus in virulent rice-infecting isolates of Magnaporthe grisea

  ML Farman, Y Eto, T Nakao, Y Tosa, H Nakayashiki, S Mayama, SA Leong

  Jan. 2002, MOLECULAR PLANT-MICROBE INTERACTIONS, 15(1) (1), 6 - 16, English

  [Refereed]

  Scientific journal


• Oat retrotransposon OARE-1 is activated in both compatible and incompatible interactions with pathogenic fungi.

  Kimura, Y, Tosa, Y, Betsuyaku, S, Sasabe, Y, Tomita, R, Murakami, J, Nakayashiki, H, Mayama, S

  Transcriptional activity of the retrotransposon OARE-1 was monitored in oat leaves infected with pathogenic fungi. The transcription of OARE-1 was upregulated in both incompatible and compatible interactions between an oat cultivar and Magnaporthe grisea isolates but more extensively in the compatible. OARE-1 was also strongly activated in a Pc2/Vb-carrying oat line inoculated with Helminthosporium victoriae. The upregulation in the Pc2/Vb-carrying oat line was reproduced by treatment with victorin C, the major compound of the host-specific toxin produced by the fungus. These results suggest that OARE-1 is responsive to various signals or stresses associated with compatibility and incompatibility.

  Phytopathological Society of Japan, 2002, J. Gen. Plant Pathol., 68(1) (1), 8 - 14, English

  [Refereed]


• OARE-1, a Ty1-copia retrotransposon in oat activated by abiotic and biotic stresses

  Y Kimura, Y Tosa, S Shimada, R Sogo, M Kusaba, T Sunaga, S Betsuyaku, Y Eto, H Nakayashiki, S Mayama

  Dec. 2001, PLANT AND CELL PHYSIOLOGY, 42(12) (12), 1345 - 1354, English

  [Refereed]

  Scientific journal


• Heat shock, copper sulfate and oxidative stress activate the retrotransposon MAGGY resident in the plant pathogenic fungus Magnaporthe grisea

  K Ikeda, H Nakayashiki, M Takagi, Y Tosa, S Mayama

  Oct. 2001, MOLECULAR GENETICS AND GENOMICS, 266(2) (2), 318 - 325, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Novel evidence for apoptotic cell response and differential signals in chromatin condensation and DNA cleavage in victorin-treated oats

  N Yao, Y Tada, P Park, H Nakayashiki, Y Tosa, S Mayama

  Oct. 2001, PLANT JOURNAL, 28(1) (1), 13 - 26, English

  [Refereed]

  Scientific journal


• Pyret, a Ty3/Gypsy retrotransposon in Magnaporthe grisea contains an extra domain between the nucleocapsid and protease domains

  H Nakayashiki, H Matsuo, Chuma, I, K Ikeda, S Betsuyaku, M Kusaba, Y Tosa, S Mayama

  Oct. 2001, NUCLEIC ACIDS RESEARCH, 29(20) (20), 4106 - 4113, English

  [Refereed]

  Scientific journal


• Induction and signaling of an apoptotic response typified by DNA laddering in the defense response of oats to infection and elicitors

  Y Tada, S Hata, Y Takata, H Nakayashiki, Y Tosa, S Mayama

  Apr. 2001, MOLECULAR PLANT-MICROBE INTERACTIONS, 14(4) (4), 477 - 486, English

  [Refereed]

  Scientific journal


• Methylation is not the main force repressing the retrotransposon MAGGY in Magnaporthe grisea

  H Nakayashiki, K Ikeda, Y Hashimoto, Y Tosa, S Mayama

  Mar. 2001, NUCLEIC ACIDS RESEARCH, 29(6) (6), 1278 - 1284, English

  [Refereed]

  Scientific journal


• Apoptotic response in defence of oats to infections and elicitors

  S Mayama, Y Tada, S Hata, Y Takata, N Yao, T Mori, Q Yang, S Betsuyaku, H Nakayashiki, Y Tosa, P Park

  2001, DELIVERY AND PERCEPTION OF PATHOGEN SIGNALS IN PLANTS, 220 - 228, English

  [Refereed]

  International conference proceedings


• Comparative analyses of the distribution of various transposable elements in Pyricularia and their activity during and after the sexual cycle

  Y Eto, K Ikeda, Chuma, I, T Kataoka, S Kuroda, N Kikuchi, LD Don, M Kusaba, H Nakayashiki, Y Tosa, S Mayama

  Jan. 2001, MOLECULAR AND GENERAL GENETICS, 264(5) (5), 565 - 577, English

  [Refereed]

  Scientific journal


• Analysis of host species specificity of Magnaporthe grisea toward wheat using a genetic cross between isolates from wheat and foxtail millet

  J Murakami, Y Tosa, T Kataoka, R Tomita, J Kawasaki, Chuma, I, Y Sesumi, M Kusaba, H Nakayashiki, S Mayama

  Oct. 2000, PHYTOPATHOLOGY, 90(10) (10), 1060 - 1067, English

  [Refereed]

  Scientific journal


• Retrotransposition of MAGGY in Pyricularia spp.

  H Nakayashiki, K Kiyotomi, Y Tosa, S Mayama

  2000, ADVANCES IN RICE BLAST RESEARCH, 15, 323 - 329, English

  [Refereed]

  International conference proceedings


• Transposition of the retrotransposon MAGGY in heterologous species of filamentous fungi

  H Nakayashiki, K Kiyotomi, Y Tosa, S Mayama

  Oct. 1999, GENETICS, 153(2) (2), 693 - 703, English

  [Refereed]

  Scientific journal


• Degenerate MAGGY elements in a subgroup of Pyricularia grisea: a possible example of successful capture of a genetic invader by a fungal genome

  H Nakayashiki, N Nishimoto, K Ikeda, Y Tosa, S Mayama

  Jul. 1999, MOLECULAR AND GENERAL GENETICS, 261(6) (6), 958 - 966, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Cauliflower mosaic virus ORF III product forms a tetramer in planta: Its implication in viral DNA folding during encapsidation

  S Tsuge, K Kobayashi, H Nakayashiki, K Mise, Furusawa, I

  1999, MICROBIOLOGY AND IMMUNOLOGY, 43(8) (8), 773 - 780, English

  [Refereed]

  Scientific journal


• Evidence for a dual strategy in the expression of cauliflower mosaic virus open reading frames I and IV

  K Kobayashi, S Tsuge, H Nakayashiki, K Mise, Furusawa, I

  1998, MICROBIOLOGY AND IMMUNOLOGY, 42(4) (4), 329 - 334, English

  [Refereed]

  Scientific journal


• Accumulation kinetics of viral gene products in cauliflower mosaic virus-infected turnip protoplasts

  K Kobayashi, H Nakayashiki, S Tsuge, K Mise, Furusawa, I

  1998, MICROBIOLOGY AND IMMUNOLOGY, 42(1) (1), 65 - 69, English

  [Refereed]

  Scientific journal


• Requirement of cauliflower mosaic virus open reading frame VI product for viral gene expression and multiplication in turnip protoplasts

  K Kobayashi, S Tsuge, H Nakayashiki, K Mise, Furusawa, I

  1998, MICROBIOLOGY AND IMMUNOLOGY, 42(5) (5), 377 - 386, English

  [Refereed]

  Scientific journal


• Genetical and physiological studies on the species-specific parasitism of Magnaporthe grisea in gramineous plants with a special reference to a cross between the Triticum isolate and the Setaria isolate

  S Mayama, Y Tosa, H Nakayashiki, M Kusaba, T Kataoka, W Kishimoto, J Murakami, K Kawashima, Y Sesumi, N Kikuchi, LD Don, K Ikeda, T Tsurushima

  1998, MOLECULAR GENETICS OF HOST-SPECIFIC TOXINS IN PLANT DISEASES, 13, 211 - 221, English

  [Refereed]

  International conference proceedings


• Accumulation of diferulic acid during the hypersensitive response of oat leaves to Puccinia coronata f sp avenae and its role in the resistance of oat tissues to cell wall degrading enzymes

  T Ikegawa, S Mayama, H Nakayashiki, H Kato

  Apr. 1996, PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 48(4) (4), 245 - 255, English

  [Refereed]

  Scientific journal


• Expression of specific resistance in crown rust oat interaction

  S Mayama, T Ikegawa, AK Kobayashi, K Zenbayashi, H Nakayashiki, H Kato

  1996, MOLECULAR ASPECTS OF PATHOGENICITY AND RESISTANCE: REQUIREMENT FOR SIGNAL TRANSDUCTION, 221 - 231, English

  [Refereed]

  International conference proceedings


• Nucleotide Sequence of a Set of cDNA Clones Derived from the Brome Mosaic Virus ATCC66 Strain and Comparison with the Russian Strain Genome.

  K. Mise, M. Mori, H. Nakayashiki, T. Koyama, T. Okuno, I. Furusawa

  The complete nucleotide sequence of a set of cDNA clones designated KU1 strain, from which infectious in vitro transcripts can be transcribed, of the ATCC66 strain of brome mosaic virus (BMV) was determined and compared with the Russian strain of BMV in both nucleotide and the deduced amino acid sequence. Each corresponding genomic RNA in the KU1 strain and the Russian strain was homologous by more than 97% in nucleotide sequence. Base substitution of the transition type was more frequent than that of the transversion type in all three genomic RNAs. Amino acid changes in three regions where significant amino acid sequence homology is shown among the Sindbis-like plant RNA viruses were mostly substitutions for amino acids categorized to similar groups. The nucleotide sequence of the 5' noncoding region in RNA1 and RNA3 was identical in the corresponding RNAs of the two strains while seven base changes were observed in RNA2. Any base change in the 3' noncoding region did not disrupt a predicted secondary structure. Two adjacent adenine residues were absent in the noncoding leader sequence of the coat protein gene of both KU1 strain and ATCC66 strain. The intercistronic oligo(A) tract in RNA3 of the ATCC66 strain was heterogeneous in length in which more than two guanine residues were observed by direct RNA sequencing, while one guanine residue was present in the RNA3 of the cDNA clone of the KU1 strain.

  The Phytopathological Society of Japan (PSJ), Aug. 1994, Annals of Phytopathological Society of Japan, 60(4) (4), 454 - 462, English

  [Refereed]

  Scientific journal


• Cauliflower mosaic virus isolate, CM1841 can be transmitted by aphids from infected plants.

  Nakayashiki H, Kobayashi K, Tsuge S, Okuno T, Furusawa I

  カリフラワーモザイクウイルス(CaMV), CM1841系統は, 昆虫媒介性に関与するウイル遺伝子(ORF II)にアミノ酸置換がある非昆虫媒介性のCaMV分離株として知られてきた。CM 1841感染葉では, ORF II産物(p18)蓄積量が昆虫媒介性のCaMVと比較して少ないが, 病徴発現初期には昆虫媒介性のCaMV系統の25〜50%程度のp18が蓄積している (Nakayashiki et al. J. Gen. Virol.74 : 2469-2472, 1993)。そこで病徴発現初期におけるCM 1841系統の昆虫媒介性を検討した。通常のアブラムシ頭数(10〜20)による検定では媒介は認められなかったが, 多頭数(200〜300)のアブラムシを用いて検討を行ったところ, これまで非昆虫媒介性と考えられていたCM 1841系統でアブラムシによる媒介が初めて確認された。

  The Phytopathological Society of Japan (PSJ), 1994, Annals of the Phytopathological Society of Japan, 60(4) (4), 496 - 500, English

  [Refereed]


• REASONS FOR THE LOW ACCUMULATION LEVEL OF APHID TRANSMISSION FACTOR PROTEIN IN INFECTED-LEAVES WITH AN APHID-NON-TRANSMISSIBLE CAULIFLOWER MOSAIC-VIRUS ISOLATE, CM1841

  H NAKAYASHIKI, S TSUGE, K KOBAYASHI, T OKUNO, FURUSAWA, I

  Nov. 1993, JOURNAL OF GENERAL VIROLOGY, 74, 2469 - 2472, English

  [Refereed]

• Evidence that Pyricularia fungi have an underground life cycle in nature

  XIANG Zikai, 中屋敷均, 池田健一

  2024, 日本植物病理学会大会プログラム・講演要旨予稿集, 2024


• Analysis of fungal localization during disease development caused by wheat seeds contaminated with Pyricularia oryzae.

  奥田樹, 内橋嘉一, 中屋敷均, 土佐幸雄, 池田健一

  2024, 日本植物病理学会報, 90(1) (1)


• Analysis of factors involved in growth inhibition and blackening of roots infected with Pyricularia grisea

  XIANG Zikai, 鶴嶋鉄, 中屋敷均, 池田健一

  2023, 日本植物病理学会大会プログラム・講演要旨予稿集, 2023


• Effect of ripening stages on the frequency of seed contamination in wheat spikes inoculated with Pyrizularia oryzae

  池田健一, 内橋嘉一, 奥田樹, 中屋敷均, 土佐幸雄

  2023, 日本植物病理学会大会プログラム・講演要旨予稿集, 2023


• In vitro production of wheat blast (Pyricularia oryzae pathotype Triticum)-infected seeds from healthy wheat seeds.

  奥田樹, 内橋嘉一, 中屋敷均, 土佐幸雄, 池田健一

  2023, 日本植物病理学会報, 89(1) (1)


• Race-cultivar specific resistance against Pyricularia oryzae on roots is not as effective as on leaves

  XIANG Zikai, 中屋敷均, 池田健一

  2022, 日本植物病理学会大会プログラム・講演要旨予稿集, 2022


• Cross-talk between sugar and lipid metabolisms during the infection process of Pyricularia oryzae.

  YANG J., XIANG Z., 中屋敷均, 池田健一

  2022, 日本植物病理学会報, 88(1) (1)


• Mechanisms of root infection in Pyricularia oryzae and P. grisea (1) Characterization of responses of gramineous plants to infection by various P. oryzae and P. grisea pathotypes.

  XIANG Z.K., 岡田大樹, 中屋敷均, 池田健一

  2022, 日本植物病理学会報, 88(1) (1)


• Evaluation of primers for quantitative PCR and LAMP to detect the Triticum pathotype of Pyricularia oryzae.

  池田健一, 内橋嘉一, 川口藍乃, 中屋敷均, 土佐幸雄

  2021, 日本植物病理学会報, 87(1) (1)


• Novel Efficient Primers for qPCR and LAMP to detect the Triticum pathotype of Pyricularia oryzae

  池田健一, 内橋嘉一, 奥田樹, 中屋敷均, 土佐幸雄

  2021, 日本植物病理学会大会プログラム・講演要旨予稿集, 2021


• Cell biology in phytopathogenic fungi during host infection: commonalities and differences

  IKEDA KENICHI, PARK Pyoyun, NAKAYASHIKI HITOSHI

  May 2019, Journal of General Plant Pathology, 85(3) (3), 163 - 173, English

  [Refereed]

  Introduction scientific journal

  Link to Kobe Univ. Repository (Kernel)


• An Argonaute gene in the fungus Magnaporthe oryzae functions as a negative regulator of RNA silencing

  Akihide Iritani, Nguyen Quyet, Van Vu Ba, Shuhei Ohkita, Kana Yokoya, Ken-ichi Ikeda, Hitoshi Nakayashiki

  Dec. 2016, GENES & GENETIC SYSTEMS, 91(6) (6), 360 - 360, English

  Summary international conference


• いもち病菌非病原力遺伝子のMultiple translocationはなぜ起こったか?欠失/再獲得仮説

  中馬いづみ, 古田純一, 草場基章, 吉田健太郎, 寺内良平, 藤田佳克, 中屋敷均, VALENT Barbara, 土佐幸雄

  15 Mar. 2012, 日本植物病理学会大会プログラム・講演要旨予稿集, 2012, 79, Japanese


• The DNA repair protein MoRad51 plays a role in de novo DNA methylaton of the LTR-retrotransposon MAGGY in the fungus Magnaporthe oryzae

  Hitoshi Nakayashiki, Van Ba Vu

  Dec. 2011, GENES & GENETIC SYSTEMS, 86(6) (6), 385 - 385, English

  Summary international conference


• The Trickster in the genome: contribution and control of transposable elements

  Hitoshi Nakayashiki

  Aug. 2011, GENES TO CELLS, 16(8) (8), 827 - 841, English

  [Refereed][Invited]

  Book review


• いもち病菌小分生子の細胞学的特徴

  中馬いづみ, 中屋敷均, 土佐幸雄

  25 Feb. 2010, 日本植物病理学会報, 76(1) (1), 61, Japanese


• いもち病菌集団における非病原力遺伝子AVR‐Piaの分布

  大窪貴子, 中馬いづみ, 吉田健太郎, 中屋敷均, 寺内良平, 土佐幸雄

  25 Feb. 2009, 日本植物病理学会報, 75(1) (1), 64, Japanese


• いもち病菌サブテロメア領域における構造変異

  中馬いづみ, 中屋敷均, 土佐幸雄

  25 Feb. 2009, 日本植物病理学会報, 75(1) (1), 65, Japanese


• RNA interference: roles in fungal biology

  Hitoshi Nakayashiki, Quoc Bao Nguyen

  Dec. 2008, CURRENT OPINION IN MICROBIOLOGY, 11(6) (6), 494 - 502, English

  [Refereed][Invited]

  Book review


• (153) Cytological Analysis of noxA and noxB Mutants of Alternaria alternata Japanese Pear Pathotype during Infection Processes(Abstracts of the Papers Presented at the Annual Meeting of the Society)

  Hyon G.-S., Morita Y., Ikeda K., Nakayashiki H., Park P.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2008, Annals of the Phytopathological Society of Japan, 74(3) (3), 207 - 207, Japanese


• (145) Functional Analysis of GATA and bHLH Transcription Factors in Magnaporthe oryzae by an RNA Silencing-based Approach(Abstracts of the Papers Presented at the Annual Meeting of the Society)

  Nakayashiki H., Kasahara S., Quoc N.B, Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2008, Annals of the Phytopathological Society of Japan, 74(3) (3), 205 - 205, Japanese


• (16) siRNA-dependent and-independent Posttranscriptional Cosuppression of the LTR-retrotransposon MAGGY in the Phytopathogenic Fungus Magnaporthe oryzae(Abstracts of the Papers Presented at the Annual Meeting of the Society)

  Murata T., Kadotani N., Tosa Y., Mayama S., Nakayashiki H.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2008, Annals of the Phytopathological Society of Japan, 74(3) (3), 171 - 171, Japanese


• (354) Functional and Phylogenic Analysis of Aldehyde Dehydrogenase Gene on Pathogenicity of Pseudomonas cichorii on Eggplant(Abstracts of the Papers Presented at the Annual Meeting of the Society)

  Tanaka M., Koyanagi M., Ohnishi K., Nakayashiki H., Kiba A., Hikichi Y.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2008, Annals of the Phytopathological Society of Japan, 74(3) (3), 261 - 261, Japanese


• 非病原力遺伝子としての機能からみたいもち病菌AVR‐Pitaの起源と進化

  中馬いづみ, 吉田健太郎, 中屋敷均, 眞山滋志, 土佐幸雄

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2008, 日本植物病理学会報, 74(3) (3), 192 - 193, Japanese


• (80)Phylogenetical Analysis of Pseudomonas fluorescens Isolates from Three Host Plants in Hyogo Prefecture(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

  Kumatani Y., Aino K., Yoshimi Y., Tosa Y., Mayama S., Nakayashiki H.

  The Phytopathological Society of Japan (PSJ), 20 Feb. 2008, Annals of the Phytopathological Society of Japan, 74(1) (1), 77 - 77, Japanese


• (20)Cytosine Methylation in Magnaporthe Oryzae is Dispensable for the Life Cycle of the Fungus in Nature(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

  Ikeda K., Tanaka M., Murata T., Shiina K., Tosa Y., Mayama S., Park P., Nakayashiki H.

  The Phytopathological Society of Japan (PSJ), 20 Feb. 2008, Annals of the Phytopathological Society of Japan, 74(1) (1), 61 - 61, Japanese


• (13)Functional Analysis of NoxB, a Gene Involved in Reactive Oxygen Species (ROS) Generation, in Alternaria alternata Japanese Pear Pathotype(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

  Hyon G. S., Morita Y., Shinogi T., Ikeda K., Nakayashiki H., Park P.

  The Phytopathological Society of Japan (PSJ), 20 Feb. 2008, Annals of the Phytopathological Society of Japan, 74(1) (1), 59 - 59, Japanese


• (21)Phenotypic Analyses of the Knock-down Mutants of 37 Calcium Signaling Proteins in Magnaporthe oryzae(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

  Quoc N. B., Tosa Y., Mayama S., Nakayashiki H.

  The Phytopathological Society of Japan (PSJ), 20 Feb. 2008, Annals of the Phytopathological Society of Japan, 74(1) (1), 61 - 61, English


• (19)Analysis of Polymorphisms in the Flanking Region of the Avirulence Gene PWL1 in Magnaporthe oryzae(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

  Tanaka M., Nakayashiki H., Mayama S., Tosa Y.

  The Phytopathological Society of Japan (PSJ), 20 Feb. 2008, Annals of the Phytopathological Society of Japan, 74(1) (1), 61 - 61, Japanese


• (22)The "Resurrection Method" for Modification of Specific Proteins in vivo : Its Applications in Filamentous Fungi(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)

  Fujiwara K., Dohi K., Mayama S., Mori M., Nakayasiki H.

  The Phytopathological Society of Japan (PSJ), 20 Feb. 2008, Annals of the Phytopathological Society of Japan, 74(1) (1), 62 - 62, Japanese


• (375) The hrp Cluster of Pseudomonas cichorii Strain SPC9081 and A Novel Pathogenicity-related Gene(Abstracts of the Papers Presented at the Annual Meeting of the Society)

  Tanaka M., Koyanagi M., Kajihara S., Hojo H., Ohnishi K., Nakayashiki H., Ashiuchi M., Kiba A., Hikichi Y.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2007, Annals of the Phytopathological Society of Japan, 73(3) (3), 275 - 275, Japanese


• (138) Functional Analysis of NoxA, a Gene Involved in Reactive Oxygen Species (ROS) Generation on the Penetration peg of Alternaria alternata Japanese Pear Pathotype(Abstracts of the Papers Presented at the Annual Meeting of the Society)

  Hyon G.-S., Shinogi T., Ikeda K., Nakayashiki H., Park R.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2007, Annals of the Phytopathological Society of Japan, 73(3) (3), 211 - 211, Japanese


• (103) Comparative Analysis of the Flanking Region of the Avirulence Gene PWL1 in Magnaporthe oryzae Eleusine Pathotype(Abstracts of the Papers Presented at the Annual Meeting of the Society)

  Tanaka M., Nakayashiki H., Mayama S., Tosa Y.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2007, Annals of the Phytopathological Society of Japan, 73(3) (3), 202 - 202, Japanese


• (108) Comprehensive Analyses of Calcium Signaling Proteins in the Rice Blast Fungus, Magnaporthe oryze, by an RNA Silencing Approach(Abstracts of the Papers Presented at the Annual Meeting of the Society)

  Quoc N.B., Tosa Y., Mayama S., Nakayashiki H.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2007, Annals of the Phytopathological Society of Japan, 73(3) (3), 203 - 203, English


• いもち病菌非病原力遺伝子AVR‐Pitaホモログのタイピング,マッピングおよび機能の比較解析

  中馬いづみ, 大鷹和也, 草場基章, 吉田健太郎, 中屋敷均, 眞山滋志, 土佐幸雄

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2007, 日本植物病理学会報, 73(3) (3), 202 - 202, Japanese


• いもち病菌小型分生子の病理学的役割を解明するための基礎調査

  細木直樹, 中馬いづみ, 篠木武, 池田健一, 中屋敷均, 眞山滋志, 土佐幸雄, PARK P

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2007, 日本植物病理学会報, 73(3) (3), 213 - 213, Japanese


• Detection of Barley Genes Conferring Resistance to the Setaria Pathotype of Magnaporthe oryzae(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

  Oomura K., Nakayasiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 2007, Annals of the Phytopathological Society of Japan, 73(1) (1), 57 - 57, Japanese


• Transfer and Elimination of the Avirulence Gene AVR-Pita in the Pyricularia Chromosomes(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

  Chuma I., Nakayashiki H., Mayama S., Tosa Y.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 2007, Annals of the Phytopathological Society of Japan, 73(1) (1), 57 - 57, Japanese


• RNA Silencing Plays a Role in Regulating the Retrotransposon MAGGY in Magnaporthe oryzae(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

  Murata T., Kadotani N., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 2007, Annals of the Phytopathological Society of Japan, 73(1) (1), 58 - 58, Japanese


• Transcriptional Control Plays a Role in the Functional Diversification of Two Dicer-like Proteins in Magnaporthe oryzae(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2006)

  Kadotani N., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 2007, Annals of the Phytopathological Society of Japan, 73(1) (1), 57 - 58, Japanese


• (139) In situ Localization of Avenanthramides and PR Gene Transcripts in Oat Leaves Infected with the Crown Rust Fungus(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

  Uchihasi K., Okamura K., Ishihara A., Park P., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Nov. 2006, Annals of the Phytopathological Society of Japan, 72(4) (4), 238 - 239, Japanese


• (152) The Course of Evolution of Magnaporthe oryzae Eleusine Pathotype Inferred from the Phylogentic Tree and the Structure around Avirulence Gene PWL1(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

  Tanaka M., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Nov. 2006, Annals of the Phytopathological Society of Japan, 72(4) (4), 242 - 242, Japanese


• (95) Specific Cleavage of Ribosomal RNA and mRNA during Victorin-induced Apoptotic Cell Death in Oats(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

  OHoat T. X., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Nov. 2006, Annals of the Phytopathological Society of Japan, 72(4) (4), 227 - 227, English


• (94) Disruption of Mitochondrial Membrane Potential Occurs in the Executive Phase of Programmed Cell Death Induced by the Host Specific Toxin, Victorin(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

  Sakamoto M., Tada Y., Tasato K., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Nov. 2006, Annals of the Phytopathological Society of Japan, 72(4) (4), 226 - 227, Japanese


• (102) RNA Silencing Is Independent of DNA Methylation in the Rice Blast Fungus, Magnaporthe oryzae(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)

  Kadotani N., Shiina K., Tosa Y., Mayama S., Nakayashiki H.

  The Phytopathological Society of Japan (PSJ), 25 Nov. 2006, Annals of the Phytopathological Society of Japan, 72(4) (4), 228 - 229, Japanese


• いもち病菌非病原力遺伝子Avr‐Pitaの座乗染色体変動過程とその機構

  中馬いづみ, 吉田健太郎, 磯部千尋, 土佐幸雄, 中屋敷均, 眞山滋志

  The Phytopathological Society of Japan (PSJ), 25 Nov. 2006, 日本植物病理学会報, 72(4) (4), 242 - 242, Japanese


• Identification of a Resistance Gene Corresponding to the Species-specific Avirulence Gene PWT3 of Magnaporthe oryzae and Evidence for their Gene-for-gene Interaction (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

  Tazawa Y., Hirata K., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 2006, Annals of the Phytopathological Society of Japan, 72(1) (1), 68 - 68, Japanese


• Analysis of Chromosome Structures of Magnaporthe oryzae by Comparative Mapping (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

  Ibaragi K., Chuma I., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 2006, Annals of the Phytopathological Society of Japan, 72(1) (1), 67 - 68, Japanese


• Comparative Analysis of Avirulence of the Magnaporthe oryzae Setaria Pathotype on Pooideae Plants (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

  Oomura K., Tanaka K., Nakayasiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 2006, Annals of the Phytopathological Society of Japan, 72(1) (1), 68 - 68, Japanese


• Identification of the Gene Responsible for Methylation of the Retrotransposon MAGGY in Magnaporthe oryzae (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)

  Shiina K., Ikeda K., Kadotani N., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 2006, Annals of the Phytopathological Society of Japan, 72(1) (1), 68 - 68, Japanese


• RNA silencing in fungi: Mechanisms and applications

  H Nakayashiki

  Oct. 2005, FEBS LETTERS, 579(26) (26), 5950 - 5957, English

  Book review

  Link to Kobe Univ. Repository (Kernel)


• (48) Analysis of Speciation Processes in Pyricularia spp. Close to P. oryzae using GCPSR(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

  Hirata K., Kusaba M., Chuma I., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2005, Annals of the Phytopathological Society of Japan, 71(3) (3), 198 - 198, Japanese


• (51) One of the Two Dicer-like Proteins in Magnaporthe oryzae is Involved in RNA Silencing Induced by Hairpin RNA(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

  Kadotani N., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2005, Annals of the Phytopathological Society of Japan, 71(3) (3), 199 - 199, Japanese


• (49) Dynamics of an Avirulence Gene Locus, Avr-Pita, in Pyricularia spp.(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

  Chuma I., Isobe C., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2005, Annals of the Phytopathological Society of Japan, 71(3) (3), 198 - 198, Japanese


• (50) Analysis of the Avirulence Gene PWL1 in Populations of the Eleusine Pathotype of Magnaporthe oryzae(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

  Tanaka M., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2005, Annals of the Phytopathological Society of Japan, 71(3) (3), 198 - 199, Japanese


• (52) Role of Rwt4, a Wheat Gene for Resistance to Avena Isolates of Magnaporthe oryzae, in the Field(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

  Hau V. T. B., Hirata K., Murakami J., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2005, Annals of the Phytopathological Society of Japan, 71(3) (3), 199 - 199, English


• (428) Regulation of the Pti5 Promoter by Multiple Multi-talented Players, Pti4, Pti5, and Pti6(Abstracts of the Paper Presented at the 2005 Annual Meeting in Shizuoka)

  Fujii T., Tsuda T., Nakayashiki H., Mayama S., Martin Gregory B.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2005, Annals of the Phytopathological Society of Japan, 71(3) (3), 300 - 300, Japanese


• (427) Promoter Analysis of the Gene Encoding Tomato Pti5, an Transcriptional Factor Belonging to the ERF Family(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)

  Nakayashiki H., Tsuda T., Martin Gregory B.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2005, Annals of the Phytopathological Society of Japan, 71(3) (3), 299 - 300, Japanese


• (9) Degradation of Ribosomal RNA Associated with Apoptotic Response of Oat Cells Triggered by the Host-Selective Toxin, Victorin(Abstracts Presented at the Meeting of the Kansai Division, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

  Hoat T. X., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 2005, Annals of the Phytopathological Society of Japan, 71(1) (1), 54 - 54, English


• (51) Gene-for-Gene Interactions Involved in the Specific Parasitism of Magnaporthe oryzae Avena Pathotype on Wheat Cultivar(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

  Hirata K., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 2005, Annals of the Phytopathological Society of Japan, 71(1) (1), 65 - 65, Japanese


• (52) A Convergent Opposing Promoters System to Explore Genome-wide Gene Function in the Rice Blast Fungus by an RNA Silencing Approach(Abstracts Presented at the Meeting of the Kansai Division, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

  Quoc N. B., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 2005, Annals of the Phytopathological Society of Japan, 71(1) (1), 66 - 66, English


• (40) Population Structure of the Eleusine Pathotype of Magnaporthe oryzae(Abstracts Presented at the Meeting of the Kansai Division, Matsuyama, October 9-10, 2004, Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

  Tanaka M., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 2005, Annals of the Phytopathological Society of Japan, 71(1) (1), 62 - 63, Japanese


• Nitric oxide-overproducing transformants of Pseudomonas fluorescens with enhanced biocontrol of tomato bacterial wilt

  WANG Yanqing, YANG Qian, TOSA Yukio, NAKAYASHIKI Hitoshi, MAYAMA Shigeyuki

  01 Feb. 2005, Journal of general plant pathology : JGPP, 71(1) (1), 33 - 38, English


• (192) Microarray Analysis of Gene Expression Dynamics Induced by Plant Growth-promoting Rizobacteria (PGPR) in Arabidopsis(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

  Ohara Y., Wang Y., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2004, Annals of the Phytopathological Society of Japan, 70(3) (3), 242 - 242, Japanese


• (4) Species Recognition Using GCPSR in Pyricularia Isolates from Various Plant Species(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

  Hirata K., Osue T., Chuma I., Tosa Y., Nakayashiki H., Mayama S

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2004, Annals of the Phytopathological Society of Japan, 70(3) (3), 191 - 191, Japanese


• (3) A Plasmid Vector for Efficient, and Effective RNA Silencing in Magnaporthe oryzae(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

  Hanada S., Kadotani N., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2004, Annals of the Phytopathological Society of Japan, 70(3) (3), 190 - 191, Japanese


• (17) Analysis of the rDNA-ITS2 and Beta-tubulin Sequences in the Blast Fungus Isolated from Centipedegrass(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

  Fuke K., Mori M., Nakayashiki H., Tosa Y., Matuura K., Mayama S., Tajimi A.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2004, Annals of the Phytopathological Society of Japan, 70(3) (3), 194 - 194, Japanese


• (5) Diversity of Karyotype and Dynamics of Avirulence Gene Lod in Pyricularia spp.(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

  Chuma I., Isobe C., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2004, Annals of the Phytopathological Society of Japan, 70(3) (3), 191 - 191, Japanese


• (47) Analysis of Apoptosis Induction Associated with Induced Resistancein Oat. 13) : Victorin Is Recognized before Its Entrance into the Cytoplasm of the Sensitive Cultivar Iowa X469(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

  Tada Y., Kusaka K., Matsubara T., Oura Y., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2004, Annals of the Phytopathological Society of Japan, 70(3) (3), 202 - 203, Japanese


• ((48) Analysis of Apoptosis Induction Associated with Induced Resistance in Oat 14) : Detection of a Victorin-binding Factor in Oat Using BIAcore(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)

  Saito R., Kusaka K., Ryu S., Tada Y., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2004, Annals of the Phytopathological Society of Japan, 70(3) (3), 203 - 203, Japanese


• (48)Modification of Pseudomonas fluorescens by Disrupting the Nitric Oxide Reductase (NOR) Gene for the Biological Control of Ralstonia solanacearum.(Abstracts Presented at the Meeting of the Kansai Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

  Wang Y., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 2004, Annals of the Phytopathological Society of Japan, 70(1) (1), 69 - 69, English


• (28) Species Recognition Using GCPSR in Magnaporthe/Pyricularia Population (Abstracts Presented at the Meeting of the Kansai Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

  Hirata K., Osue J., Chuma I., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 2004, Annals of the Phytopathological Society of Japan, 70(1) (1), 63 - 64, Japanese


• (29) Karyotypic Diversity of the Blast Fungus Pyricularia spp. (Abstracts Presented at the Meeting of the Kansai Division) (Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2003)

  Isobe C., Chuma I., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 2004, Annals of the Phytopathological Society of Japan, 70(1) (1), 64 - 64, Japanese


• エンバクにおける細胞死機構研究のこれまで

  中屋敷 均, 土佐 幸雄, 眞山 滋志

  2004, 植物病の探求． 高松進ら編 「植物病の探求」, 48-53, Japanese

  Others


• Studies on Species-specific Parasitism of Magnaporthe oryzae (13). Reduction of Pathogenicity against Adult Resistance in M. orayzae Hybrids(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

  Murakami J., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2003, Annals of the Phytopathological Society of Japan, 69(3) (3), 269 - 269, Japanese


• Geographic Origin of Perennial Ryegrass Isolates of Magnaporthe oryzae and Their Potential for Causing Wheat Blast(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

  Nakagawa S., Tosa Y., Hirata K., Chuma I., Isobe C., Nakayashiki H., Mori N., Wakar Uddin, Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2003, Annals of the Phytopathological Society of Japan, 69(3) (3), 269 - 270, Japanese


• Meiotic Behavior of a Supernumerary Chromosome and Its Rearrangement in Magnaporthe oryzae(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

  Chuma I., Tosa Y., Taga M., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2003, Annals of the Phytopathological Society of Japan, 69(3) (3), 237 - 237, Japanese


• Induction of Chlorosis, ROS Formation and Cell Death of Novel Toxins Isolated from Magnaporthe grisea(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

  Minami Y., Tsurushima T., Sakamoto M., Miyagawa H., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2003, Annals of the Phytopathological Society of Japan, 69(3) (3), 233 - 233, Japanese


• Detection of Small Interfering RNAs (siRNAs) in Magnaporthe grisea(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)

  Kadotani N., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2003, Annals of the Phytopathological Society of Japan, 69(3) (3), 237 - 237, Japanese


• RNA Silencing in a Phytopathogenic Fungus, Magnaparthe grisea

  Kadotani N., Nakayashiki H., Tosa Y., Mayama S.

  RNAサイレンシングは動物,植物,および糸状菌などの幅広い真核生物が持つ外来核酸に対する防御機構と考えられている.我々はコムギから分離されたいもち病菌Br48にGFP遺伝子を導入し,強いGFP緑色蛍光を示す株を得た.この形質転換体にGFP RNAをセンス,アンチセンス,ヘアピンなどの様々な形で転写するコンストラクトをそれぞれ導入し,GFP蛍光の増減を調査した結果,ヘアピン構造をとるGFP RNAを転写するコンストラクトが高頻度でGFP蛍光の抑制を誘導することが明らかとなった.これらGFPの発現が抑制された株ではGFP mRNAの蓄積が顕著に低下していることがノーザン解析により示された.また,GFPのサイレンシングが観察された株ではGFPヘアピンRNAに相当する3.2kbのバンドが常に観察され,両者の間に相関が認められた.以上の結果は,いもち病菌にRNAサイレンシング機構が存在することを示し,ゲノム解析が進展している本菌の逆遺伝学的な遺伝子解析に有用な手法となることが期待される.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 2003, Annals of the Phytopathological Society of Japan, 69(1) (1), 51 - 51, Japanese


• Analysis of Apoptosis Induction Associated with Induced Resistance in Oat. : 13) Cytological Analysis of Calciumregulated Reactive Oxygen Species Generation and Cell Death

  Takahashi S., Park P., Tada Y., Nakayashiki H., Tosa Y., Mayama S.

  植物の抵抗性発現およびアポトーシスの誘導にCa^<2+>およびROSは重要な役割を担っている.シロイヌナズナの細胞膜在性NADPHオキシダーゼAtrbohはCa^<2+>結合部位を有することが報告され,Ca^<2+>とROS生成には深い相関性があることが明らかとなっている.しかし現在までにCa^<2+>に制御されるROS生成の詳細な細胞学的解析は行われていない.そこで本研究ではカルシウムイオノフォアA23187がエンバク葉肉細胞に誘導する細胞死およびROS生成についての解析を行った.A23187処理したエンバクでは,明瞭なDNAラダー,グロマチンの凝集が認められ,EM-TUNELポジティブであり,アポトーシス様の細胞死が起こっていることが示唆された.塩化セリウム法により,細胞膜およびミトコンドリア,葉緑体,パーオキシゾームなどの細胞内小器官で強いH_2O_2の蓄積が認められた.NADPHオキシダーゼ阻害剤であるDPIを供処理すると,細胞膜でのH_2O_2蓄積および細胞死が抑制されたが,これに伴い細胞内小器官でのH_2O_2蓄積も抑制された.このことより,細胞膜でのO_2-生成が細胞内小器官での二次的なROS生成を誘導することが示唆された.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 2003, Annals of the Phytopathological Society of Japan, 69(1) (1), 55 - 55, Japanese


• RNA silencing in the blast fungus Magnaporthe grisea

  Nakayashiki Hitoshi

  Kobe University, 2003, The science reports of Faculty of Agriculture, Kobe University, 27, 50 - 53, Japanese


• (77)Pyret, a Ty3/Gypsy Retrotransposon in Magnaporthe grisea Contains an Extra Domain between the Nucleocapsid and Protease Domains

  Nakayashiki H., Matuo H., Chuma I., Ikeda K., Betsuyaku S., Kusaba M., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2002, Annals of the Phytopathological Society of Japan, 68(2) (2), 176 - 177, Japanese


• (57)Studies on Species-Specific Parasitism of M. grisea (12) Gene-for-Gene Interactions Involved in the Specific Parasitism of Avena and Triticum Isolates

  Takabayashi N., Tosa Y., Oh H. S., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2002, Annals of the Phytopathological Society of Japan, 68(2) (2), 171 - 171, Japanese


• (78)Comparative Analysis of Genome Structure in Magnaporthe grisea using RFLP Mapping

  Chuma I., Isoda K., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2002, Annals of the Phytopathological Society of Japan, 68(2) (2), 177 - 177, Japanese


• (27)Analysis of Apoptosis Induction Associated with Induced Resistance in Oat. 11) Hypersensitive Cell Death of Oat Leaf Cells Induced by the Crown Rust Fungus is an Apoptotic Type

  Yao N., Park P., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2002, Annals of the Phytopathological Society of Japan, 68(2) (2), 163 - 163, Japanese


• (28)Analysis of Apoptosis Induction Associated with Induced Resistance in Oat. 12) Purification and Characterization of 28K Nuclease Induced by Victorin in Oat

  Kusaka K., Shigemi T., Tada Y., Hata S., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2002, Annals of the Phytopathological Society of Japan, 68(2) (2), 163 - 164, Japanese


• (29)Analysis of Pathogenesis-related Compounds in Pyricularia spp. (6) Isolation of Chlorosis-inducing Toxins from an Avena Isolate of Pyricularia grisea

  Minami Y., Tsurushima T., Miyagawa H., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2002, Annals of the Phytopathological Society of Japan, 68(2) (2), 164 - 164, Japanese


• (324)Involvement of Apoptosis-Like Cell Death in Disease Development in Lettuce Leaves Inoculated with P. cichorii

  Sangawa Y., Kiba A., Hojo H., Ohnishi K., Yao N., Park P., Nakayashiki H., Tosa Y., Mayama S., Hikichi Y.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2002, Annals of the Phytopathological Society of Japan, 68(2) (2), 243 - 243, Japanese


• Sequence Analysis of a Transposon Cluster in Magnaporthe grisea(Abstracts Presented at the Meeting of the Kansai Division)

  Chuma I., Matsuo H., Betsuyaku S., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Apr. 2002, Annals of the Phytopathological Society of Japan, 68(1) (1), 90 - 90, Japanese


• Temperature-sensitivity of the Species-specific Parasitism of Magnaporthe isolates from Oat and Wheat, and Identification of an AFLP Marker Linked to a Fungal Gene Conditioning the Specificity on Oat(Abstracts Presented at the Meeting of the Kansai Division)

  Oh H.S., Tomita R., Murakami J., Takabayashi N., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Apr. 2002, Annals of the Phytopathological Society of Japan, 68(1) (1), 90 - 90, Japanese


• The Distribution and Structure of the AVR1-CO39 Avirulence Gene in Pyricularia spp.

  Eto Y., Nakao T., Tosa Y., Chuma I., Nakayashiki H., Farman M.L., Leong S.A., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2001, Annals of the Phytopathological Society of Japan, 67(2) (2), 181 - 181, Japanese


• Studies on Species-Specific Parasitism of Magnaporthe grisea(11). Genetic Analysis of Pathogenicity on Foxtail Millet of Progenies Derived from the Cross between a Setaria Isolate and a Triticum Isolate

  Murakami J., Tomita R., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2001, Annals of the Phytopathological Society of Japan, 67(2) (2), 181 - 181, Japanese


• Increase of Disease Resistance during Senescence of Oat Primary Leaves

  Betsuyaku S., Ikenaga E., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2001, Annals of the Phytopathological Society of Japan, 67(2) (2), 122 - 122, Japanese


• Analysis of Apoptosis Induction Associated with Induced Resistance in Oat. (10) Victorin-induced Susceptibility Requires Calcium Influx Elicited by the Recognition of the Toxin

  Tada Y., Betsuyaku S., Oura Y., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2001, Annals of the Phytopathological Society of Japan, 67(2) (2), 122 - 122, Japanese


• Occurrence of RIP(Repeat Induced Point Mutation)during Hybridization in Magnaporthe grisea

  Ikeda K., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2001, Annals of the Phytopathological Society of Japan, 67(2) (2), 130 - 130, Japanese


• Cloning of Genes for Phytoalexin Synthesis in Oats

  Yang Q., Betsuyaku S., Imai S., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2001, Annals of the Phytopathological Society of Japan, 67(2) (2), 121 - 121, Japanese


• Analysis of Apoptosis Induction Associated with Induced Resistance in Oat (9) The Role of Mitochondria in Oat Apoptotic Cells

  Yao N., Park P., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2001, Annals of the Phytopathological Society of Japan, 67(2) (2), 121 - 122, Japanese


• Methylation is not the Responsible Mechanism Repressing the Retrotransposon MAGGY in Magnaporthe grisea

  Nakayashiki H., Ikeda K., Hashimoto Y., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2001, Annals of the Phytopathological Society of Japan, 67(2) (2), 129 - 130, Japanese


• Distribution of Oat Retrotransposon OARE-1 in Cereals and Its Expression Profile during Infection with Pathogenic Fungi

  Kimura Y., Sogo R., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2001, Annals of the Phytopathological Society of Japan, 67(2) (2), 136 - 137, Japanese


• Involvement of gacA Gene in the Suppression of Tomato Bacterial Wilt by Pseudomonas fluorescens FPT9601 :

  ZHANG Liqun, YANG Qian, TOSA Yukio, NAKAYASHIKI Hitoshi, MAYAMA Shigeyuki

  Pseudomonas fluorescens FPT9601, a plant growth-promoting rhizobacterium (PGPR) isolated from tomato rhizosphere, can protect tomato (Lycopersicon esculentum Mill) from bacterial wilt disease caused by Ralstonia solanacearum. This strain produces antibiotics 2,4-diacetylphloroglucinol (2,4-DAPG) and hydrogen cyanide (HCN). It also produces proteases and uncharacterized siderophores (Sid). A mutant strain SM2214, obtained by Tn5 insertion, did not produce 2,4-DAPG, HCN or proteases, but overproduced Sid. Marker-exchange mutagenesis confirmed that a single transposon insertion caused the multiple phenotypic changes of this mutant. Complementation of the mutant with a 1.3-kb DNA fragment that was amplified from genomic DNA of the wild-type P. fluorescens strain by PCR could restore the lost functions of the mutant strain. Nucleotide sequencing revealed that the fragment contained a 642-bp open reading frame (ORF) highly homologous to the regulator responser gene gacA. The in vitro anti-bacterium test and plant protection experiment under greenhouse conditions indicated that the gacA gene played an important role in the suppression of tomato bacterial wilt disease. (Received November 20, 2000 ; Accepted January 19, 2001)

  Phytopathological Society of Japan, 2001, Journal of general plant pathology : JGPP, 67(2) (2), 134 - 143, English


• Analysis of Apoptosis Induction Associated with Induced Resistance in Oat : 8)EM-TUNEL Identifies Oat Apoptotic Cells(Abstracts Presented at the Meeting of the Kansai Division)

  Yao N., Park P., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 2000, Annals of the Phytopathological Society of Japan, 66(3) (3), 288 - 289, Japanese


• Studies on Species-specific Parasitism of Magnaporthe grisea(9). : Genetic Analysis of Pathogenicity on Wheat of Progenies Derived from the Cross between an Oryza Isolate and a Triticum Isolate(Abstracts Presented at the Meeting of the Kansai Division)

  Tamba H., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 2000, Annals of the Phytopathological Society of Japan, 66(3) (3), 281 - 281, Japanese


• Studies on Species-specific Parasitism of Magnaporthe grisea(10). : Genetic Analysis of Pathogenicity in an F_1 population Derived from a Cross between a Brachiaria Isolate and a Triticum Isolate(Abstracts Presented at the Meeting of the Kansai Division)

  Kuroda S., Urashima A.S., Don L.D., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 2000, Annals of the Phytopathological Society of Japan, 66(3) (3), 281 - 282, Japanese


• Cloning of Retrotransposons in Oat and Their Transcriptional Activity(Abstracts Presented at the Meeting of the Kansai Division)

  Kimura Y., Sogo R., Sunaga T., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 2000, Annals of the Phytopathological Society of Japan, 66(3) (3), 291 - 291, Japanese


• Analysis of Pathogenesis-related Compounds in Pyricularia spp. : (5)Pathogenicity Factors in Triticum and Avena Isolates of Pyricularia grisea(Abstracts Presented at the Meeting of the Kansai Division)

  Minami Y., Tsurushima T., Kawashima K., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 2000, Annals of the Phytopathological Society of Japan, 66(3) (3), 281 - 281, Japanese


• Studies on Species-Specific Parasitism of Magnaporthe grisea (8) Establishment of a System with an Avena Isolate

  Takabayashi N., Tomita R., Don L.D., Minami Y., Tamba H., Oh H., Tosa Y., Tsurushima T., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2000, Annals of the Phytopathological Society of Japan, 66(2) (2), 120 - 120, Japanese


• Analysis of Pathogenesis-related Compounds in Pyricularia spp. (4) Ubiquity of Pyrichalasin H Production in Isolates from the Genus Digitaria

  Tsurushima T., Kusaba M., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2000, Annals of the Phytopathological Society of Japan, 66(2) (2), 120 - 120, Japanese


• Degeneration of Transposable Elements by RIP (Repeat-Induced Point Mutation)-Like Process in Panicum Isolates of Pyricularia grisea RIP (repeat-induced point mutation)

  Ikeda K., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2000, Annals of the Phytopathological Society of Japan, 66(2) (2), 120 - 120, Japanese


• Cloning of GacA Gene from Pseudomonas fluorescens FPT9601

  Zhang L. Q., Tosa Y., Nakayashiki H.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2000, Annals of the Phytopathological Society of Japan, 66(2) (2), 137 - 137, English


• Analysis of Apoptosis Induction Associated with Induced Resistance in Oat 7) : Induction of Apoptosis in Response to Virus, Bacteria and Fungi

  Imai S., Yao N., Tada Y., Park P., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2000, Annals of the Phytopathological Society of Japan, 66(2) (2), 113 - 113, Japanese


• Studies on Species-Specific Parasitism of Magnaporthe grisea(7) : A Blast Disease-Resistance System Conserved between Different Tribes in Gramineous Plants

  Murakami J., Tomita R., Kawasaki J., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2000, Annals of the Phytopathological Society of Japan, 66(2) (2), 119 - 120, Japanese


• Analysis of Apoptosis Induction Associated with Induced Resistence in Oat 6) Cell Signals for Induction of DNA Ladders and Chromatin Condensation

  Yao N., Tada Y., Park P., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 2000, Annals of the Phytopathological Society of Japan, 66(2) (2), 113 - 113, Japanese


• Signal Mediators for Phytoalexin Production in Defense Response of Oats Elicited by Victorin as a Specific Elicitor :

  TADA Yasuomi, HATA Shingo, NAKAYASHIKI Hitoshi, TOSA Yukio, MIYAMA Shigeyuki

  Signals mediating phytoalexin(PA)production were analyzed in primary leaves of oats cv.Iowa X469 treated with an elicitor victorin. Production of the PA avenanthramide A was inhibited by DPI, an inhibitor of NADPH oxidase/nitric oxide synthase(NOS)and the NOS inhibitors L-NMMA and 1, 3-PBIT. However, catalase and superoxide dismutase (SOD)hardly suppressed it. From the data, NO functions as a major reactive oxygen species in signal transduction leading to PA production in the defense response of oats. EGTA, verapamil and ruthenium red inhibited PA production, suggesting that Ca^<2+> influx into the cytoplasm and intracellular Ca^<2+> movement are involved in the defense response. Trifluoperazine, a calmodulin function inhibitor, and K-252a, a serine/threonine kinase inhibitor, also suppressed the accumulation, whereas okadaic acid, a serine/threonine phosphatase inhibitor, did not suppress it, suggesting the involvement of calmodulin and protein kinase, but not of phosphatase in PA production.

  Phytopathological Society of Japan, 2000, Journal of general plant pathology : JGPP, 66(3) (3), 185 - 190, English


• Pathogenicity, Mating Ability and DNA Restriction Fragment Length Polymorphisms of Pyricularia Populations Isolated from Gramineae, Bambusideae and Zingiberaceae Plants :

  KATO Hajime, YAMAMOTO Masaaki, YAMAGUCHI-OZAKI Tomona, KADOUCHI Hiroyuki, IWAMOTO Yutaka, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki, MORI Naoki

  Eighty-five Pyricularia isolates were collected from 29 host species of Gramineae, Bambusideae and Zingiberaceae plants sampled in Brazil, Uganda, Ivory Coast, India, Nepal, China, Indonesia and Japan.These isolates were compared on the basis of pathogenicity, mating ability and restriction fragment length polymorphisms with single-copy DNA probes.Based on the pathogenicity to eight differential gramineous plants, these isolates were classified into seven pathotypes:finger millet type, foxtail millet type, common millet type, rice type, crabgrass type, Italian ryegrass / weeping lovegrass type, and non-cereal / grass type.Genetic variation among these isolates was assessed by RFLP analysis with two restriction enzymes and nine single-copy DNA probes isolated from a finger millet strain.An UPGMA dendrogram based on the RFLPs revealed that the 85 isolates could be classified into seven major groups.Isolates from cereal crops(finger millet, foxtail millet, common millet, wheat and rice)and a grass, Brachiaria plantaginea, were clustered into a single group.They were further divided into six subgroups corresponding to the pathotypes.Among cereal crop isolates only an isolate from pearl millet was located into a different group.The remaining isolates were clustered into five groups designated as the crabgrass group, the buffelgrass and jungle rice group, the rice cutgrass, knotroot bristlegrass and Setaria tomentosa group, the bamboo and bamboo grass group and the Zingiber mioga group.The isolates from cereal crops were generally capable of mating with finger millet strains and constituted a closed mating compatibility group.These results suggested that the isolates from cereal crops from a single group with a common ancestor although they are pathogenic to taxonomically diverse plants.A combined analysis of the pathogenicity and genetic similarity suggested that the transmission of M.grisea isolates occurs in natural agroecosystems between finger millet and Eleusine africana, goosegrass or Bambusa arundinacea, between foxtail millet and green bristlegrass, and between rice and tail fescue, Italian ryegrass, sweet vernalgrass, reed canarygrass or Oryza longistaminata.

  Phytopathological Society of Japan, 2000, Journal of general plant pathology : JGPP, 66(1) (1), 30 - 47, English


• Origin of the Blast Fungus Occurring on Perennial Ryegrass in Golf Courses (2) : Analysis of DNA Fingerprint and rDNA (Abstracts Presented at the Meeting of the Kansai Division)

  Tamba H., Don L. D., Urashima A. S., Eto Y., Tosa Y., Nakayashiki H., Tanaka A., Tani T., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 1999, Annals of the Phytopathological Society of Japan, 65(6) (6), 673 - 673, Japanese


• Analysis of Apoptosis Induction Associated with Induced Resistance in Oats 5) : Antithetic Effects H_2O_2 and No Productions on Induction of PA Accumulation and DNA-laddering (Abstracts Presented at the Meeting of the Kansai Division)

  Mori T., TAda Y., Yao N., Park P., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 1999, Annals of the Phytopathological Society of Japan, 65(6) (6), 691 - 691, Japanese


• Studies on species-specific Parasitism of Magnaporthe grisea (6) : Identification of Genes for Pathogenicity on Wheat of Progenies from a Setaria Isolate×a Triticum Isolate and Analysis of Their Function in Cellular Responses

  Tomita R., Murakami J., Kawasaki J., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 1999, Annals of the Phytopathological Society of Japan, 65(6) (6), 690 - 690, Japanese


• Possible Involvement of NO Production Pathway in Inhibition of Phytoalexin Production of Oats Polyamines (Abstracts Presented at the Meeting of the Kansai Division)

  Yamamoto R., Tada Y., Ikenaga E., Mori T., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 1999, Annals of the Phytopathological Society of Japan, 65(6) (6), 691 - 691, Japanese


• Genetic Diversity in Pyricularia Isolates from Various Hosts Revealed by Polymorphisms of Nuclear Ribosomal DNA and the Distribution of the MAGGY Retrotransposon

  KUSABA Motoaki, ETO Yukiko, DON Le Dinh, NISHIMOTO Natsuka, TOSA Yukio, NAKAYASHIKI Hitoshi, MAYAMA Shigeyuki

  Pyricularia isolates from various gramineous phants were subjected to RFLP and sequence analyses of rDNA to clarify their genetic relationships. Based on rDNA-RFLP patterrns produced by digestion with two restriction enzymes, 71 isolates from 27 plant species were grouped into 13 rDNA types. To estimate their genetic relatedness, a UPGMA dendrogram was constructed based on the rDNA-RFLP profiles. The ITS2 regions of 13 representative isolates were also amplified by PCR and directly sequenced to construct another dendrogram. In both dendrograms, isolates from agronomically important crops (rice, foxtail millet, common millet, finger millet, wheat and oats) and their relatives were classified into a single cluster. Isolates from weeds, such as crabgrass and buffelgrass, were located outside the crop isolate cluster, and Japanese bamboo or bamboo grass isolates were farthest from the crop isolate cluster. In surveying the distribution of a retrotransposon, MAGGY, in these isolates, MAGGY was present in multiple copies in isolates from rice, foxtail millet and six other hosts, which formed a single subcluster within the crop isolate cluster. Multiple copies of MAGGY were also detected in an isolate from buffelgrass, which was distant from the other MAGGY carriers in ther DNA dendrograms. These results suggest that the horizontal transfer of MAGGY occurre during the differentiation of Pyricularia.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 1999, Annals of the Phytopathological Society of Japan, 65(6) (6), 588 - 596, English


• Population Structure of the Rice Blast Pathogen in Vietnam

  DON Le Dinh, TOSA Yukio, NAKAYASHIKI Hitoshi, MAYAMA Shigeyuki

  ベトナムの主要な2つの稲作地帯, Red River Delta(北部)およびMekong River Delta(南部)から採集したイネいもち病菌計78菌糸を用いて, 両地域における本病菌の個体群構造を比較検討した。レトロトランスポゾンMAGGYをプローブとしたDNAフィンガープリンティングの結果, 北部の菌株は4つのリネージ(VL1-VL4)に分かれたが, 南部の菌株はこれらとは異なる単一リネージ(VL5)に分類された。このことから, 北部の個体群の方が遺伝的多様性に富むことが示唆された。さらに, 判別品種を用いた病原性検定の結果, 両集団におけるレース構成は大きく異なり, 共通のレースは見いだされなかった。以上のことから, 遺伝的にも病原性においても両個体群の構造は大きく異なることが示唆された。

  The Phytopathological Society of Japan (PSJ), 25 Aug. 1999, Annals of the Phytopathological Society of Japan, 65(4) (4), 475 - 479, English


• Molecular Analysis of the Wheat Blast Population in Brazil with a Homolog of Retrotransposon MGR583

  URASHIMA Alfredo S., HASHIMOTO Yoko, DON Le D., KUSABA Motoaki, TOSA Yukio, NAKAYASHIKI Hitoshi, MAYAMA Shigeyuki

  We have identified a family of dispersed repetitive DNA sequences in the genome of a Magnaporthegrisea isolate from finger millet (Eleusine coracana). Southern blot analyses showed that this element is present in a moderate copy number (30-40 copies) in the genome of blast isolates from wheat (Triticum aestivum). DNA sequence data suggested that this element contains a region highly homologous to the reverse transcriptase domain of MGR583, a poly A-type retrotransposon. Using the reverse transcriptase domain of this element as a molecular probe, the genetic structure of the wheat blast population was examined. DNA fingerprinting analyses revealed that the wheat-infecting isolates constitute a separate, single lineage of their own, suggesting that they are derived from a single origin.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 1999, Annals of the Phytopathological Society of Japan, 65(4) (4), 429 - 436, English


• Origin of the Blast Fungus Occurring on Perennial Ryegrass in Golf Courses Suggested from Their Host Range, Sexual Compatibility, and DNA Fingerprint Pattern

  Tamba H., Urashima A. S., Don L. D., Tosa Y., Nakayashiki H., Tanaka A., Tani T., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1999, Annals of the Phytopathological Society of Japan, 65(3) (3), 342 - 342, Japanese


• Activation of Transposition of MAGGY in Pyricularia oryzae : Analysis of Promoter Activity Using an LTR-GUS Construct

  Takagi M., Ikeda K., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1999, Annals of the Phytopathological Society of Japan, 65(3) (3), 341 - 342, Japanese


• Analysis of Pathogenesis-related Compounds in Pyricularia spp. : (3) : Detection of Pyrichalasin H in Crabgrass Infected with Pyricularia grisea and Suppression of Plant Resistance by Pyrichalasin H

  Tsurushima T., Kawashima K., Murakami J., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1999, Annals of the Phytopathological Society of Japan, 65(3) (3), 328 - 329, Japanese


• Analysis of Apoptosis Induction Associated with Induced Resistance in Oats : (3) : Nucleases Activated by Victorin Treatment

  Hata S., Tada Y., Takata Y., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1999, Annals of the Phytopathological Society of Japan, 65(3) (3), 321 - 321, Japanese


• Analysis of Apoptosis Induction Associated with Induced Resistance in Oat : (4) : Ultrastructural Observation of Nuclei

  Yao N., Park P., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1999, Annals of the Phytopathological Society of Japan, 65(3) (3), 321 - 321, Japanese


• Population Structure of the Rice Blast Fungus in Japan Examined by DNA Fingerprinting

  DON Le Dinh, KUSABA Motoaki, URASHIMA Alfredo S., Tosa Yukio, NAKAYASHIKI Hitoshi, MAYAMA Shigeyuki

  DNA fingerprinting with multilocus probes, MAGGY and MGR586, was conducted to investigate the population structure of the rice blast fungus Magnaporthe grisea in Japan. Two-hunredseventy-eight field isolates collected during 1993-1997 and 22 archival isolates collected in 1976 from various cultivars and locations were examined. Two fingerprint groups were identified at the 70% similarity level in each collection. A one-to-one correspondence was found between the groups in field isolates and the groups in archival isolates. These result ssuggest that two discrete lineages exist in the rice blast population. They were temporarily designated as JL1 and JL2 (Japanese Lineage). JL1 was a predominant lineage representing 97% and 77% of the field and archival isolates, respectively, and present through out Japan. In addition, these two lineages corresponded to two of the five lineages previously detected in collections made before 1960, suggesting that the structure of the rice blast population in Japan had drastically changed during 1960-1976. The two lineages showed a very similar virulence spectrum, and no relationship could be recognized between lineages and pathotypes. MAGGY revealed robust groups which were similar to those revealed by MGR586, although the two elements differ in character and structure, suggesting that MAGGY could be used as an alternative probe for monitoring the population dynamics of the rice blast pathogen.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 1999, Annals of the Phytopathological Society of Japan, 65(1) (1), 15 - 24, English


• (5) Studies on Species-specific Parasitism of Magnaporthe grisea (5) Evidence for Transposition of MAGGY and Evaluation of Activity of Other Repetitive Elements in Progenies from the Cross, a Setaria Isolate × a Triticum Isolate (Abstracts Presented at the Meeting of the Kansai Division, 1998)

  Eto Y., Chuma I., Ikeda K., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 1998, Annals of the Phytopathological Society of Japan, 64(6) (6), 616 - 616, Japanese


• (4) DNA-finger-printing Analysis of Lineage of the Rice Blast Fungus (3) Population Structure of Field Isolates in Vietnam (Abstracts Presented at the Meeting of the Kansai Division, 1998)

  Don L. D., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 1998, Annals of the Phytopathological Society of Japan, 64(6) (6), 615 - 616, English


• (39) Studies on the Transposition of Retrotransposon MAGGY Introduced into New Hosts (Abstracts Presented at the Meeting of the Kansai Division, 1998)

  Hashimoto Y., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 1998, Annals of the Phytopathological Society of Japan, 64(6) (6), 624 - 625, Japanese


• (95) Analysis of Pathogenesis-related Compounds in Pyricularia spp. (2) Phytotoxins Produced by Wheat Isolate

  Kawashima K., Tsurushima T., Murakami J., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 1998, Annals of the Phytopathological Society of Japan, 64(4) (4), 350 - 351, Japanese


• (104) Analysis of Apoptosis Induction Associated with Induced Resistance in Oats (1) Induction of Apoptosis of Infection and Elicitors

  Hata S., Tada Y., Takata Y., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 1998, Annals of the Phytopathological Society of Japan, 64(4) (4), 353 - 353, Japanese


• (96) Possible Involvement of DNA Methylation in the Regulation of MAGGY-like Elements Found in the Panicum miliaceum Isolate of Magnaporthe grisea

  Nishimoto N., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 1998, Annals of the Phytopathological Society of Japan, 64(4) (4), 351 - 351, Japanese


• (97) DNA-fingerprinting Analysis of Lineage of the Rice Blast Fungus (2) Population Structure of Field Isolates in Japan

  Don L.D., Kusaba M., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 1998, Annals of the Phytopathological Society of Japan, 64(4) (4), 351 - 351, Japanese


• (106) Chitinase as PR-protein in Oats and Elicitor Activity of Oligosaccharides Released by the Enzyme from Infection Hyphae of Crown Rust Fungi

  Takata Y., Tada Y., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 1998, Annals of the Phytopathological Society of Japan, 64(4) (4), 353 - 353, Japanese


• (107) Analysis of Genes Activated in Resistance Responce of Oats

  Betsuyaku S., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 1998, Annals of the Phytopathological Society of Japan, 64(4) (4), 353 - 354, Japanese


• (99) Distribution of Transposable Elements and Double-stranded RNA in Pyricularia spp.

  Ikeda K., Chuma I., Eto Y., Kusaba M., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 1998, Annals of the Phytopathological Society of Japan, 64(4) (4), 351 - 352, Japanese


• (100) Studies on Species-specific Parasitism of Magnaporthe grisea (3) Genetic Analysis of Pathogenicity on Wheat of Progenies Derived from the Cross between a Setaria Isolate and a Triticum Isolate

  Murakami J., Sesumi Y., Tomita R., Kusaba M., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 1998, Annals of the Phytopathological Society of Japan, 64(4) (4), 352 - 352, Japanese


• (101) Studies on Species-specific Parasitism of Magnaporthe grisea (4) Construction of RFLP Map Using an F_1 Population Derived from the Cross between a Setaria Isolate and a Triticum Isolate

  Chuma I., Murakami J., Eto Y., Kusaba M., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 1998, Annals of the Phytopathological Society of Japan, 64(4) (4), 352 - 352, Japanese


• (94) Analysis of Pathogenesis-related Compounds in Pyricularia spp. (1) Pyrichalasin H Production and Pathogenisity of Magnaporthe grisea on Crabgrass

  Tsurushima T., Murakami J., Kawashima K., Nikaidou K., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 1998, Annals of the Phytopathological Society of Japan, 64(4) (4), 350 - 350, Japanese


• (105) Analysis of Apoptosis Induction Associated with Induced Resistance in Oats (2) Involvement of Ca^<2+>, Activated Oxygen, Protease and Endonuclease

  Tada Y., Hata S., Takata Y., Nakayashiki H., Tosa Y., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 1998, Annals of the Phytopathological Society of Japan, 64(4) (4), 353 - 353, Japanese


• (98) Molecular Analysis of Population Structure and Origin of the Wheat Blast Fungus

  Urashima A.S., Hashimoto Y., Don L.D., Kusaba M., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 1998, Annals of the Phytopathological Society of Japan, 64(4) (4), 351 - 351, Japanese


• (108) Cloning of Retro-transposons in Oat by RT-PCR

  Shimada S., Kusaba M., Tosa Y., Nakayashiki H., Mayama S.

  The Phytopathological Society of Japan (PSJ), 25 Aug. 1998, Annals of the Phytopathological Society of Japan, 64(4) (4), 354 - 354, Japanese


• Natural Infection of Wild Grass Species with Rice Blast Fungus Suggested by DNA Fingerprinting

  KUSABA Motoaki, DON Le Dinh, URASHIMA Alfredo S., ETO Yukiko, TOSA Yukio, NAKAYASHIKI Hitoshi, YAMAMOTO Masaaki, MAYAMA Shigeyuki

  MAGGYをブローブとしたDNAフィンガープリント分析により, 世界各地から採集したイネいもち病菌と日本産野生イネ科植物いもち病菌との遺伝的類縁関係を調査した。供試菌から得られたDNAフィンガープリントに基づきクラスター分析を行ったところ, 日本産と外国産イネ菌の類縁性は, 27%以下であった。一方, 日本産ネズミムギ, ハルガヤ, クサヨシ, オニノウシノケグサ各菌は日本産イネ菌に対して80%以上の高い類縁性を示した。以上の結果から, これら日本産の野生イネ科植物の菌はイネ菌がこれら植物に感染したものであると推定される。

  The Phytopathological Society of Japan (PSJ), 25 Apr. 1998, Annals of the Phytopathological Society of Japan, 64(2) (2), 125 - 128, English


• (4) Transposition of Genetically Introduced Retorotransposon MAGGY in Pyricularia spp. Isolated from Various Plants

  KIYOTOMI K., NAKAYASHIKI H., TOSA Y., MAYAMA S.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 1997, Annals of the Phytopathological Society of Japan, 63(6) (6), 507 - 508, Japanese


• (5) Analysis on the Occurrence of Natural Infection of Wild Gramineous Plants with the Rice Blast Fungus Based on DNA-fingerprinting Using MAGGY as a Probe

  KUSABA M., ETO Y., TOSA Y., NAKAYASHIKI H., MAYAMA S.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 1997, Annals of the Phytopathological Society of Japan, 63(6) (6), 508 - 508, Japanese


• (216) Genetic Relatedness among Pyricularia Species Isolated from Gramineous Plants Estimated by Nuclear Ribosomal DNA RFLP Analysis Using Restriction Enzymes That Recognize Four-nucleotide Long(Abstracts of the Papers Presented at the Annual Meeting of the Society)

  KUSABA M., YAMAMOTO M., ETO Y., TOSA Y., NAKAYASHIKI H., MAYAMA S.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1997, Annals of the Phytopathological Society of Japan, 63(3) (3), 245 - 245, Japanese


• (217) Distribution of Retrotransposon MAGGY in Pyricularia Species : with the Special Reference to the Possibility of Its Horizontal Transfer(Abstracts of the Papers Presented at the Annual Meeting of the Society)

  ETO Y., KUSABA M., YAMAMOTO M., TOSA Y., NAKAYASHIKI H., MAYAMA S.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1997, Annals of the Phytopathological Society of Japan, 63(3) (3), 245 - 245, Japanese


• (220) Studies on Species-specific Parasitism of Magnaporthe grisea : II. Rearrangement at MAGGY Loci in Progenies Derived from a Cross between a Wheat Isolate and a Foxtail Millet Isolate(Abstracts of the Papers Presented at the Annual Meeting of the Society)

  KATAOKA T., TOSA Y., NAKAYASHIKI H., MAYAMA S.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1997, Annals of the Phytopathological Society of Japan, 63(3) (3), 246 - 246, Japanese


• (218) DNA-fingerprinting Analysis of Lineage of Rice Blast Fungus : I. Analysis of Isolates from 7 Prefectures in Japan Using MAGGY as a Probe(Abstracts of the Papers Presented at the Annual Meeting of the Society)

  DON L. D., NAKAYASHIKI H., TOSA Y., MAYAMA S.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1997, Annals of the Phytopathological Society of Japan, 63(3) (3), 245 - 246, English


• (219) Relationship between the Activation of Transposition and the Formation of Intracellular Complex Related to the Retrotransposon, MAGGY(Abstracts of the Papers Presented at the Annual Meeting of the Society)

  NAKAYASHIKI H., AWA T., TOSA Y., MAYAMA S.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1997, Annals of the Phytopathological Society of Japan, 63(3) (3), 246 - 246, Japanese


• (156) Phytotoxic Substance in Spore Germination Fluid of Magnmporthe grisea(Abstracts of the Papers Presented at the Annual Meeting of the Society)

  MURAKAMI J., NAKAYASHIKI H., TOSA Y., MAYAMA S.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1997, Annals of the Phytopathological Society of Japan, 63(3) (3), 230 - 230, Japanese


• Introduction of Chitinase Gene Derived from Kurthia zopfii into Strawberry Plants Using Biolistic Method Enhances Resistance to Colletotrichum fragariae Brooks

  YAO N., NAKAYASHIKI N., TOSA Y., MAYAMA S.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 1996, Annals of the Phytopathological Society of Japan, 62(6) (6), 622 - 623, Japanese


• Cloning of Nuclear Ribosomal DNA of Pyricularia species Isolated from Gramineous Plants

  KUSABA M., YAMAMOTO M., TOSA Y., NAKAYASHIKI H., MAYAMA S.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 1996, Annals of the Phytopathological Society of Japan, 62(6) (6), 617 - 617, Japanese


• Possible Release of Elicitor-active Oligosaccharides from Infection Hyphae of Crown Rust Fungi by Chitinase

  TADA Y., NAKAYASHIKI H., TOSA Y., KATO H., MAYAMA S.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1996, Annals of the Phytopathological Society of Japan, 62(3) (3), 290 - 291, Japanese


• Activation of Retrotransposon-like Element in Panicum miliaceum Strain of Magnaporthe grisea

  NAKAYASHIKI H., TOSA Y., NISHIMOTO N., TAKAGI M., KATO H., MAYAMA S.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1996, Annals of the Phytopathological Society of Japan, 62(3) (3), 293 - 293, Japanese


• Retrotransposon-like Element Found in Genomic DNA of Magnaporthe grisea (Panicum miliaceum Strain), Which Are Homologous to the Retrotransposon, MAGGY

  NISHIMOTO N., NAKAYASHIKI H., TOSA Y., KATO H., MAYAMA S.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1996, Annals of the Phytopathological Society of Japan, 62(3) (3), 293 - 293, Japanese


• Parasitic Specialization of Magnaporthe grisea : 10. RFLP Analysis of Genomic DNA (4)

  YAMAMOTO M., NAKAYASHIKI H., TOSA Y., MAYAMA S., KATO H.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1996, Annals of the Phytopathological Society of Japan, 62(3) (3), 293 - 294, Japanese


• Studies on Species-specific Parasitism of Magnaporthe grisea II. : Species-specific Virulence Factor Produced in Spore Germination Fluid of Setaria italica Strain

  KISHIMOTO W., NAKAYASHIKI H., TOSA Y., KATO H., MAYAMA S.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1996, Annals of the Phytopathological Society of Japan, 62(3) (3), 288 - 288, Japanese


• Studies on Speciesspecific Parasitism of Magnaporthe grisea : I. Analysis of Pathogenicity and Retrotransposon in the Progenies of Wheat Strain×Foxtailmillet Strain

  KATAOKA T., NAKAYASHIKI H., TOSA Y., KATO H., MAYAMA S.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1996, Annals of the Phytopathological Society of Japan, 62(3) (3), 293 - 293, Japanese


• Distribution of Retrotransposon MAGGY in Pyricularia Species

  TOSA Yukio, NAKAYASHIKI Hitoshi, HYODO Hideyuki, MAYAMA Shigeyuki, KATO Hajime, LEONG S. A.

  さまざまな植物から分離されたいもち病菌におけるレトロトランスポゾンMAGGYの分布を調査した。本因子はイネ菌, アワ菌, エノコログサ菌には多コピー存在したが, コムギ菌, シコクビエ菌, オヒシバ菌, メヒシバ菌, Digitaria horizontalis菌, およびミョウガ菌には全く存在しなかった。以上のことから, イネ菌はアワ菌ならびにエノコログサ菌と近縁であることが示唆された。

  The Phytopathological Society of Japan (PSJ), 25 Dec. 1995, Annals of the Phytopathological Society of Japan, 61(6) (6), 549 - 554, English


• Parasitic Specialization of Magnaporthe grisea : 9. RFLP Analysis of Genomic DNA

  YAMAMOTO M., NAKAYASHIKI H., YAMAGUCHI T., MORI N., MAYAMA S., KATO H.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1995, Annals of the Phytopathological Society of Japan, 61(3) (3), 231 - 231, Japanese


• Distribution of Retrotransposon Maggy in Blast Fungi from Various Gramineous Plants

  TOSA Y., NAKAYASHIKI H., MAYAMA S., KATO H.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1995, Annals of the Phytopathological Society of Japan, 61(3) (3), 231 - 231, Japanese


• Identification of a RAPD Marker Linked to the Crown Rust Resistance and Victorin Sensitive Gene Pc-2/Vb in Oats

  MAYAMA S., ZENBAYASHI K., NAKAYASHIKI H., KATO H.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1995, Annals of the Phytopathological Society of Japan, 61(3) (3), 234 - 234, Japanese


• Virulence Factors in Spore Germination Fluids of Pyricularia oryzae

  MURAKAMI J., MARUHASHI Y., NAKAYASHIKI H., MAYAMA S., KATO H.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1995, Annals of the Phytopathological Society of Japan, 61(3) (3), 250 - 250, Japanese


• Effects of Specinc and Non-Specific Elicitors on Plasmamembrane-Bound ATPase in Oats

  OONISHI T., OKANO R., MAYAMA S., NAKAYASHIKI H., KATO H.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1995, Annals of the Phytopathological Society of Japan, 61(3) (3), 246 - 246, Japanese


• Chitinases Induced with Elicitor Treatment in Oats

  KIMURA Y., YAMANAKA M., MAYAMA S., NAKAYASHIKI H., KATO H.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1995, Annals of the Phytopathological Society of Japan, 61(3) (3), 246 - 246, Japanese


• Tagging of Pc-2/Vb, the Crown Rust Resistance and Victorin-Sensitive Gene in Oats, by Using RAPD Markers

  MAYAMA S, ZENBAHASHI K, NAKAYASHIKI H, HOSAKA K, KATO H, TANI T

  The Phytopathological Society of Japan (PSJ), 25 Dec. 1994, Annals of the Phytopathological Society of Japan, 60(6) (6), 769 - 769, Japanese


• (17) Establishment of a Suspension Culture System of Oat Cells for Avenalumin Production Induced by Various Elicitors

  KOBAYASHI A.K, MAYAMA S, NAKAYASHIKI H, KATO H

  The Phytopathological Society of Japan (PSJ), 25 Dec. 1994, Annals of the Phytopathological Society of Japan, 60(6) (6), 769 - 770, English


• Relationship between Locally-Induced Indigestibility by Degrading Enzyme of Cell Walls at Infection Sites and Wall-Bound Peroxidase Catalyzing Diferulic Acid Formation in Crown Rust-Oats Interactions

  IKEGAWA T, MAYAMA S, NAKAYASHIKI H, KATO H, TANI T

  The Phytopathological Society of Japan (PSJ), 25 Dec. 1994, Annals of the Phytopathological Society of Japan, 60(6) (6), 769 - 769, Japanese


• Cauliflower Mosaic Virus, CM1841 Isolate is Transmitted by Aphids from Infected Leaves

  NAKAYASHIKI H, KOBAYASHI K, TSUGE S, OKUNO T, FURUSAWA I

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1994, Annals of the Phytopathological Society of Japan, 60(3) (3), 384 - 384, Japanese


• Parasitic Specialization of Magnaporthe grisea 8. : RFLP Analysis of Genomic DNA (2)

  MORI N., YAMAMOTO M., NAKAYASHIKI H., OZAKI T., MAYAMA S., KATO H.

  The Phytopathological Society of Japan (PSJ), 25 Jun. 1994, Annals of the Phytopathological Society of Japan, 60(3) (3), 346 - 347, Japanese


• Replication of Cauliflower Mosaic Virus ORF I Mutants in Turnip Protoplasts

  TSUGE Seiji, KOBAYASHI Kappei, NAKAYASHIKI Hitoshi, OKUNO Tetsuro, FURUSAWA Iwao

  We succeeded in infecting turnip protoplasts with a cloned cauliflower mosaic virus (CaMV) DNA, pCa122, which contains 1.2 copy of CaMV genomic DNA and a plasmid expressing open reading frame (ORF) VI products (pEXP6) using polyethylene glycol. Fluorescent antibody staining showed that up to 50% of protoplasts were infected. It was difficult to detect the progeny DNA and the viral protein in protoplasts inoculated with pCa122 alone. Co-transfection with the plasmid pEXP6 produced larger fluorescing specks in each infected protoplasts and increased the accumulation of the progeny DNA and some other viral proteins to detectable levels. Using this protoplast system, three CaMV ORF I insertional mutants which were not infectious on turnip plants were tested for their infectivity on turnip protoplasts. Viral DNA and products accumulated in infected protoplasts to the same extent of the wild type DNA-infected protoplasts. These results indicate that ORF I product is not required for multiplication of CaMV in protoplasts, but is indispensable for infection on whole plants, strongly supporting that ORF I product is involved in cell-to-cell movement of CaMV.

  The Phytopathological Society of Japan (PSJ), 25 Feb. 1994, Annals of the Phytopathological Society of Japan, 60(1) (1), 27 - 35, English


• Characterization of In Vitro Recombinant Cauliflower Mosaic Viruses in Aphid Transmissibility

  NAKAYASHIKI Hitoshi, KOBAYASHI Kappei, TSUGE Seiji, OKUNO Tetsuro, FURUSAWA Iwao

  Aphid transmissible Japanese isolate of cauliflower mosaic virus M (CaMV-M) was cloned into pUC19 and its ORF II region was sequenced. Comparison of this sequence with that of CM1841 (non-aphid transmissible isolate of CaMV) showed that there were nine base changes, four of which gave rise to alterations in the predicted amino acid sequence. Characterization of in vitro recombined hybrid viruses between CaMV-M and CM1841 showed that 5' half of ORF II was responsible for the lack of aphid transmissibility in CM1841 and this region influenced the amount of accumulation of ORF II product in the infected leaves.

  The Phytopathological Society of Japan (PSJ), 25 Dec. 1991, Annals of the Phytopathological Society of Japan, 57(5) (5), 634 - 640, English

• Histone modification in Pyricularia oryzae: mechanisms and its possible involvement in genome evolution

  Kobayashi, N, Dnag, T.A, Pham, T.M.K, Ikeda, K, Nakayashiki, H

  Joint work, Phytopathological Society of Japan, Aug. 2022


• What is a gene?

  Hitoshi Nakayashiki

  Single work, 講談社, Apr. 2022, Japanese, ISBN: 4065277663


• Essential Genetics and Genomics

  Hartl, Daniel L., 中村, 千春, 岡田, 清孝

  Joint work, 細菌とバクテリオファージの遺伝学, 化学同人, Jan. 2021, Japanese, ISBN: 9784759820485


• 植物たちの戦争

  NAKAYASHIKI HITOSHI

  Joint work, 講談社, Mar. 2019, Japanese

  General book


• 科学と非科学 その正体を探る

  NAKAYASHIKI HITOSHI

  Single work, 講談社, Mar. 2019, Japanese

  General book


• ウイルスは生きている

  NAKAYASHIKI HITOSHI

  Single work, 講談社現代新書, Mar. 2016, Japanese

  General book


• RNA silencing in filamentous fungi: from basic to applications

  Nguyen, Q.B, NAKAYASHIKI HITOSHI

  Joint work, Springer-Verlag, Jan. 2015, English

  Scholarly book


• 生命のからくり

  NAKAYASHIKI HITOSHI

  Single work, 講談社現代新書, Jun. 2014, Japanese

  General book


• 細胞死の新展開. 新版 分子レベルからみた植物の耐病性 島本功、渡辺雄一郎編 pp.118-125

  中屋敷 均, 眞山 滋志

  Joint work, 秀潤社, 2004, Japanese

  Scholarly book


• いもち病菌におけるRNAサイレンシング機構 自他識別と応答のバイオフロンティア 植物感染生理談話論文集. 高橋英樹,羽柴輝良編 pp.53-62

  中屋敷 均, 角谷 直樹, 花田 周吾, 土佐 幸雄, 眞山 滋志

  Joint work, 日本植物病理学会, 2004, Japanese

  Scholarly book


• RNA silencing in the phytopathogenic fungus Magnaporthe grisea. In: Rice Blast: Interaction with Rice and Control(eds. S. Kawasaki) pp.15-21

  NAKAYASHIKI Hitoshi, KADOTANI Naoki, TOSA Yukio, MAYAMA Shigeyuki

  Joint work, Kluwer academic pub., 2004, English

  Scholarly book

• Spatio-temporal regulation of morphological differentiation in Pyricularia oryzae.

  IKEDA KENICHI, 藤原 有紗, NAKAYASHIKI HITOSHI

  The 8th International Rice Blast Conference, May 2019, English, Chingdu, China, International conference

  Poster presentation


• コムギいもち病菌の生産する小分子RNAの体系的な特徴付け

  梅崎 佑樹, Quyet Nguyen, IKEDA KENICHI, NAKAYASHIKI HITOSHI

  平成31年度日本植物病理学会大会, Mar. 2019, Japanese, つくば国際会議場, Domestic conference

  Oral presentation


• コムギいもち病菌Ourmia-like virusesの2種のサテライトRNA様因子

  李 唯衣, 大北 修平, IKEDA KENICHI, 鈴木 信弘, NAKAYASHIKI HITOSHI

  平成31年度日本植物病理学会大会, Mar. 2019, Japanese, つくば国際会議場, Domestic conference

  Oral presentation


• Olpidium virulentusの休眠胞子におけるミラフィオリレタスビックベインウイルスの局在

  IKEDA KENICHI, 岩本 豊, 松浦 克成, 西口 真嗣, NAKAYASHIKI HITOSHI

  平成31年度日本植物病理学会大会, Mar. 2019, Japanese, つくば国際会議場, Domestic conference

  Oral presentation


• ヴァイロコントロールー菌類ウイルスを利用した病害防除法ー

  IKEDA KENICHI, 坂口 耕太, 伊東 祐弥, 兼松 聡子, NAKAYASHIKI HITOSHI

  日本共生生物学会 第２回大会, Nov. 2018, Japanese, 神戸大学, Domestic conference

  Poster presentation


• いもち病菌の組織特異的感染戦略

  岡田 大樹, NAKAYASHIKI HITOSHI, IKEDA KENICHI

  日本共生生物学会 第２回大会, Nov. 2018, Japanese, 神戸大学, Domestic conference

  Poster presentation


• ヒストンメチル基転移酵素MoSET1が制御するいもち病菌リン酸化酵素の機能解析

  濱根 美穂子, IKEDA KENICHI, NAKAYASHIKI HITOSHI

  平成30年度日本植物病理学会関西部会, Sep. 2018, Japanese, 山口大学, Domestic conference

  Oral presentation


• いもち病菌のイネ根における宿主特異的寄生性評価

  岡田 大樹, NAKAYASHIKI HITOSHI, IKEDA KENICHI

  平成30年度日本植物病理学会関西部会, Sep. 2018, Japanese, 山口大学, Domestic conference

  Oral presentation


• いもち病菌の脂質・糖代謝における付着器形成シグナル経路の関与．

  原田 叡人, 中本 知里, Ngyyen Hanh, NAKAYASHIKI HITOSHI, IKEDA KENICHI

  平成30年度日本植物病理学会大会, Mar. 2018, Japanese, 神戸国際会議場, Domestic conference

  Oral presentation


• MoAgo3アルゴノートはMoAgo1とのsiRNAの競合によりRNAサイレンシングを負に制御する

  Nguyen Quyet, 松原 愛, IKEDA KENICHI, 鈴木 信弘, NAKAYASHIKI HITOSHI

  平成29年度 日本植物病理学会関西部会, Sep. 2017, Japanese, 大阪府立大学, Domestic conference

  Oral presentation


• Building blocks法によるいもち病菌の脂質代謝遺伝子群の機能解析

  IKEDA KENICHI, 翠川 陽大, 前田 健太郎, NAKAYASHIKI HITOSHI

  平成29年度 日本植物病理学会関西部会, Sep. 2017, Japanese, 大阪府立大学, Domestic conference

  Oral presentation


• 土壌からのミラフィオリレタスビックベインウイルスのqRT-PCR法による検出

  井上 海, 松浦 克成, 岩本 豊, 西口 真嗣, IKEDA KENICHI, NAKAYASHIKI HITOSHI

  平成２９年度日本植物病理学会大会, Apr. 2017, Japanese, 盛岡, Domestic conference

  Oral presentation


• Olpidium virulentusの遊走子放出に及ぼす土壌pHの影響

  IKEDA KENICHI, 井上 海, 岩本 豊, 相野 公孝, 松浦 克成, 前川 和正, NAKAYASHIKI HITOSHI, 西口 真嗣

  平成２９年度日本植物病理学会大会, Apr. 2017, Japanese, 盛岡, Domestic conference

  Oral presentation


• Three argonaute protein and their associated sRNAs in Pyricularia oryzae

  Nguyen Q, Iritani S, Vu BV, Ohkita K, IKEDA KENICHI, NAKAYASHIKI HITOSHI

  7th International Rice Blast Conference, Oct. 2016, English, The Bellevue Manila, Philippines, International conference

  Oral presentation


• Hydrophobin in a wheat isolate of Pyricularia oryzae: Mpg1, but not Mhp1, is essential for adhesion and pathogenicity

  IKEDA KENICHI, Kitaoka H, Inoue K, NAKAYASHIKI HITOSHI, PARK Pyoyun

  7th International Rice Blast Conference, Oct. 2016, English, The Bellevue Manila, Philippines, International conference

  Poster presentation


• 白紋羽病菌におけるウイルス伝搬能を獲得した変異株の細胞学的解析

  伊東 祐弥, 兼松 聡子, NAKAYASHIKI HITOSHI, IKEDA KENICHI

  日本植物病理学会関西部会, Sep. 2016, Japanese, 静岡県コンベンションアーツセンター グランシップ, Domestic conference

  Oral presentation


• コムギいもち病菌における3種の新規ourmiavirus様マイコウイルスの同定

  大北 修平, IKEDA KENICHI, 鈴木 信弘, NAKAYASHIKI HITOSHI

  日本植物病理学会関西部会, Sep. 2016, Japanese, 静岡県コンベンションアーツセンター グランシップ, Domestic conference

  Oral presentation


• いもち病菌におけるオートファジー誘導機構の解明（２）オートファジーと付着器形成過程におけるシグナル経路の検証

  中本 知里, Nguyen Hieu Hanh, NAKAYASHIKI HITOSHI, IKEDA KENICHI

  日本植物病理学会関西部会, Sep. 2016, Japanese, 静岡県コンベンションアーツセンター グランシップ, Domestic conference

  Oral presentation


• イネ科植物いもち病菌ゲノムに存在する3種のArgonauteにロードされるsRNAの比較解析

  入谷 彰英, Nguyen Quyet, 大北 修平, 横矢 佳奈, IKEDA KENICHI, NAKAYASHIKI HITOSHI

  日本植物病理学会関西部会, Sep. 2016, Japanese, 静岡県コンベンションアーツセンター グランシップ, Domestic conference

  Oral presentation


• 糸状菌におけるウイルス伝搬能を獲得した変異株の特性解析

  伊東 祐弥, 森田 雄一, 上森 喬大, 井上 加奈子, NAKAYASHIKI HITOSHI, IKEDA KENICHI

  平成28年度植物感染生理談話会, Aug. 2016, Japanese, シーパル須磨（神戸）, Domestic conference

  Poster presentation


• オートファジーと付着器形成過程におけるシグナル経路の解明

  中本 知里, 井上 加奈子, NAKAYASHIKI HITOSHI, IKEDA KENICHI

  平成28年度植物感染生理談話会, Aug. 2016, Japanese, シーパル須磨（神戸）, Domestic conference

  Poster presentation


• いもち病菌におけるオートファジー誘導機構の解明（１）各種シグナル物質添加による影響評価

  中本 知里, NAKAYASHIKI HITOSHI, IKEDA KENICHI

  平成28年度日本植物病理学会大会, Mar. 2016, Japanese, 岡山コンベンションセンター, Domestic conference

  Oral presentation


• Ralfuranones secreted by Ralstonia solanacearum function as intercellular secondary messengers during its biofilm formation

  森 友花, 大西 秀幸, 井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 木場 章範, 大西 浩平, 甲斐 建次, 曳地 康史

  第３０回 日本微生物生態学会, Oct. 2015, English, 亀城プラザ 土浦市, Domestic conference

  Poster presentation


• ポリリン酸化合物処理による糸状菌病害防除法の開発

  大崎 裕介, 北川 博子, NAKAYASHIKI HITOSHI, IKEDA KENICHI

  平成２７年度日本植物病理学会関西部会, Sep. 2015, Japanese, あわぎんホール徳島県郷土文化会館, Domestic conference

  Oral presentation


• 青枯病菌のＢＦ形成機構とその病原性への役割

  曳地 康史, 森 友花, 石川 詩歩, 東本 周樹, 井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 木場 章範, 大西 浩平, 甲斐 建次

  平成２７年度植物感染生理談話会, Aug. 2015, Japanese, 松山市, Domestic conference

  [Invited]

  Invited oral presentation


• 糸状菌における不和合性反応を制御する遺伝子の解明

  伊東 祐弥, 森田 雄一, 上森 喬大, 井上 加奈子, NAKAYASHIKI HITOSHI, IKEDA KENICHI

  平成２７年度植物感染生理談話会, Aug. 2015, Japanese, 松山市, Domestic conference

  Poster presentation


• いもち病菌の病原性発現過程におけるオートファジーの役割

  中本 知里, 井上 加奈子, NAKAYASHIKI HITOSHI, IKEDA KENICHI

  平成２７年度植物感染生理談話会, Aug. 2015, Japanese, 松山市, Domestic conference

  Poster presentation


• いもち病菌ゲノムのエピジェネティックな遺伝子制御

  Pham Thi Minh Kieu, 井上 義博, Vu Van Ba, IKEDA KENICHI, NAKAYASHIKI HITOSHI

  平成２７年度植物感染生理談話会, Aug. 2015, Japanese, 松山市, Domestic conference

  [Invited]

  Invited oral presentation


• Ralstonia solanacearum OE1-1株のバイオフィルム形成にレクチンRS-IILは必要である

  森 友花, 井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 大西 秀幸, 甲斐 建次, 木場 章範, 大西 浩平, 曳地 康史

  平成２７年度植物感染生理談話会, Aug. 2015, Japanese, 松山市, Domestic conference

  Poster presentation


• 青枯病菌のバイオフィルム形成への菌体外多糖の関与

  森 友花, 東本周樹, 井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 木場 章範, 大西 浩平, 曳地 康史

  日本植物病理学会大会, Mar. 2015, Japanese, 明治大学, Domestic conference

  Oral presentation


• 次世代シーケンサを用いた白紋羽病菌の細胞質不和合性反応における発現変動遺伝子の解析

  森田雄一, 上森喬大, 井上加奈子, NAKAYASHIKI HITOSHI, 兼松聡子, IKEDA KENICHI

  日本植物病理学会大会, Mar. 2015, Japanese, 明治大学, Domestic conference

  Oral presentation


• いもち病菌の根部感染過程にオートファジーは必須ではない．

  IKEDA KENICHI, 井上加奈子, NAKAYASHIKI HITOSHI, Michael Kershaw, Nicholas Talbot

  日本植物病理学会大会, Mar. 2015, Japanese, 明治大学, Domestic conference

  Oral presentation


• MAPキナーゼPmk1およびMps1はH3K4ヒストンメチル基転移酵素MoSET1の上流で機能する．

  グエン ハン, ファム キウ, IKEDA KENICHI, NAKAYASHIKI HITOSHI

  日本植物病理学会大会, Mar. 2015, Japanese, 明治大学, Domestic conference

  Oral presentation


• furanone合成酵素をコードするralA遺伝子は青枯病菌のバイオフィルム形成に関与する．

  森 友花, 大西秀幸, 井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 木場 章範, 大西 浩平, 曳地 康史

  日本植物病理学会大会, Mar. 2015, Japanese, 明治大学, Domestic conference

  Oral presentation


• イネ科植物いもち病菌のArgonaute3はRNAサイレンシングを 負に制御する

  IRITANI shoei, Yokoya Kana, NAKAYASHIKI HITOSHI

  糸状菌分子生物学コンファレンス, Nov. 2014, Japanese, 仙台, Domestic conference

  Poster presentation


• The MAP kinase, Pmk1 is epistatic to MoSET1, H3K4 methyltransferase for infection-related morphogenesis in Magnaporthe oryzae

  Nguyen Hieu Hanh, Kieu Thi Minh Pham, Ikeda Kenichi, NAKAYASHIKI HITOSHI

  糸状菌分子生物学コンファレンス, Nov. 2014, Japanese, 仙台, Domestic conference

  Poster presentation


• 白紋羽病菌の和合性及び不和合性反応についての細胞学的・分子生物学的解析

  森田 雄一, 上森 喬大, 井上加奈子, NAKAYASHIKI HITOSHI, 兼松聡子, IKEDA KENICHI

  日本植物病理学会関西部会, Sep. 2014, Japanese, 富山大学, Domestic conference

  Oral presentation


• The MAP kinase, Pmk1 is epistatic to MoSET1, H3K4 methyltransferase for infection-related morphogenesis in Magnaporthe oryzae

  Nguyen Hieu Hanh, Kieu Thi Minh Pham, Ikeda Kenichi, NAKAYASHIKI HITOSHI

  日本植物病理学会関西部会, Sep. 2014, Japanese, Domestic conference

  Oral presentation


• Chromosomal Localization Patterns of Avirulence Effector Genes in the Rice Blast Fungus Pyricularia oryzae and Related Species

  CHUMA IZUMI, 角 圭人, 土井 佑介, 村井 俊子, 草場 基章, YOSHIDA KENTAROU, 寺内 良平, NAKAYASHIKI HITOSHI, TOSA YUKIO

  IS-MPMI 2014 International Congress, Jul. 2014, English, Rhodes, Greece, International conference

  Poster presentation


• トマト植物の細胞間隙に接する細胞表面で青枯病菌はバイオフィルムを形成する

  森 友花, 井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 尾上 孝利, 木場 章範, 大西 浩平, 曳地 康史

  日本植物病理学会全国大会, Jun. 2014, Japanese, 札幌コンベンションセンター, Domestic conference

  Oral presentation


• Infection-related gene expression largely depends on MoSET1, the histone lysine methyltransferases catalyzing H3K4 methylation in Magnaporthe oryzae

  Kieu Pham Thi Minh, Yoshihiro Inoue, Ikeda Kenichi, NAKAYASHIKI HITOSHI

  日本植物病理学会, Jun. 2014, Japanese, 札幌, Domestic conference

  Oral presentation


• MoSET1ヒストンメチルカトランスフェラーゼに依存したいもち病菌の感染器官形成時の遺伝子発現

  Pham Kieu, 井上 喜博, IKEDA KENICHI, NAKAYASHIKI HITOSHI

  日本植物病理学会全国大会, Jun. 2014, Japanese, Domestic conference

  Oral presentation


• いもち病菌におけるヒストンリジンメチルトランスフェラーゼの役割

  Pham Kieu, 井上 喜博, Vu Ba, Nguyen Quoc, IKEDA KENICHI, NAKAYASHIKI HITOSHI

  糸状菌分子生物学コンファレンス, Nov. 2013, Japanese, Domestic conference

  Oral presentation


• Histone methyltransferase genes play roles in the pathogenicity of the rice blast fungus, Magnaporthe oryzae.

  Nakayashiki Hitoshi

  Sino-Japan Joint Symposium of Plant Pathology, Aug. 2013, English, Beijing, China, International conference

  [Invited]

  Invited oral presentation


• Histone lysine methyltransferases in Magnaporthe oryzae are involved in various aspects of infection and pathogenesis.

  Pham, K. T. M, Vu, B. V, Nguyen, Q. B, IKEDA KENICHI, Nakayashiki Hitoshi

  6th International Rice Blast Conference 2013, Aug. 2013, English, International conference

  Oral presentation


• 植物病原糸状菌感染時において単子葉植物と双子葉植物で誘導されるパピラの形成機序に関する細胞学的比較解析

  井上 加奈子, 宮田 奈緒, 毛呂 恵, NAKAYASHIKI HITOSHI, PARK Pyoyun, IKEDA KENICHI

  日本植物病理学会関西部会, 2013, Japanese, Domestic conference

  Poster presentation


• 糸状菌の細胞質不和合性反応におけるプログラム細胞死機構の解析

  上森 喬大, 井上 加奈子, 北澤 大知, 木田 千晶, NAKAYASHIKI HITOSHI, 兼松 聡子, IKEDA KENICHI

  日本土壌微生物学会, 2013, Japanese, Domestic conference

  Poster presentation


• 糸状菌の細胞質不和合性反応におけるプログラム細胞死機構の解析

  上森 喬大, 井上 加奈子, 木田 千晶, NAKAYASHIKI HITOSHI, 兼松 聡子, IKEDA KENICHI

  日本植物病理学会関西部会, 2013, Japanese, Domestic conference

  Poster presentation


• リアルタイム定量PCR法による菌核病生物防除剤Coniothyrium minitansの追跡調査

  宿南 良, 岩本 豊, 小川 宗和, NAKAYASHIKI HITOSHI, IKEDA KENICHI

  日本植物病理学会関西部会, 2013, Japanese, Domestic conference

  Poster presentation


• ヒストンメチル転移酵素群はイネ科植物いもち病菌の感染において重要な役割を果たす

  Pham Kieu, Vu Ba, Nguyen Quoc, IKEDA KENICHI, 井上 喜博, TOSA YUKIO, NAKAYASHIKI HITOSHI

  日本植物病理学会大会, 2013, Japanese, Domestic conference

  Oral presentation


• ナシ黒斑病菌のRacAの病原性及び胞子発芽に及ぼす影響

  森田 雄一, 玄 康洙, 伊藤 理紗, NAKAYASHIKI HITOSHI, PARK Pyoyun, IKEDA KENICHI

  日本植物病理学会大会, 2013, Japanese, Domestic conference

  Oral presentation


• いもち病菌の胞子発芽時におけるオートファジー誘導の経時的解析

  井上 加奈子, 坂井田 彩野, 兼松 聡子, NAKAYASHIKI HITOSHI, PARK Pyoyun, IKEDA KENICHI

  日本植物病理学会大会, 2013, Japanese, Domestic conference

  Oral presentation


• Multiple host adhesion factors in Magnaporthe oryzae –potential target for disease control-

  IKEDA KENICHI, Kitagawa H, Inoue K, Shimoi S, Osaki Y, Park Pyoyun, Nakayashiki Hitoshi

  6th International Rice Blast Conference 2013, 2013, English, International conference

  Poster presentation


• MoSET1, histone methyltransferase responsible for methylation of histone H3 lysine 4 in Magnaporthe oryzae is required for both activation of gene expression during infection-related morphogenesis of the fungus

  Pham Kieu, Vu Ba, IKEDA KENICHI, NAKAYASHIKI HITOSHI

  日本植物病理学会関西部会, 2013, Japanese, Domestic conference

  Oral presentation


• Macroautophagy during appressorium morphogenesis in Magnaporthe oryzae

  Inoue K, IKEDA KENICHI, Nakayashiki Hitoshi

  6th International Rice Blast Conference 2013, 2013, English, International conference

  Poster presentation


• Histone lysine methyltransferases in Magnaporthe oryzae are involved in various aspects of infection and pathogenesis

  Pham Kieu, Vu Ba, Nguyen Quoc, IKEDA KENICHI, Nakayashiki Hitoshi

  10th International Congress of Plant Pathology, 2013, English, International conference

  [Invited]

  Invited oral presentation


• Autophagy-inducing condition during infection process of Magnaporthe oryzae

  Inoue K, Nakayashiki Hitoshi, IKEDA KENICHI

  10th International Congress of Plant Pathology, 2013, English, International conference

  Poster presentation


• Attachment of airborne pathogens to their host; a potential target for disease control

  IKEDA KENICHI, Kitagawa H, Shimoi S, Inoue K, Osaki Y, Nakayashiki Hitoshi, Park Pyoyun

  10th International Congress of Plant Pathology, 2013, English, International conference

  [Invited]

  Invited oral presentation


• Multiple translocation of the AVR-Pita effector gene among chromosomes of the rice blast fungus Magnaporthe oryzae and related species.

  CHUMA IZUMI, C. Isobe, Y. Hotta, K. Ibaragi, N. Futamata, M. Kusaba, K. Yoshida, R. Terauchi, Y. Fujita, NAKAYASHIKI HITOSHI, B. Valent, TOSA YUKIO

  IS-MPMI 2012 International Congress, Jul. 2012, English, Kyoto, Japan, International conference

  Oral presentation


• Multiple translocation of the AVR-Pita effector gene among chromosomes of the rice blast fungus Magnaporthe oryzae and related species

  CHUMA IZUMI, C. Isobe, Y. Hotta, K. Ibaragi, N. Futamata, M. Kusaba, K. Yoshida, R. Terauchi, Y. Fujita, NAKAYASHIKI HITOSHI, B. Valent, TOSA YUKIO

  The 2nd Korea-Japan Joint Symposium 2012, Mar. 2012, English, International conference

  Poster presentation


• ナシ黒斑病菌の付着器局在性NADPH oxidase Bは侵略力発揮に必須である

  森田 雄一, 玄 康洙, NAKAYASHIKI HITOSHI, 稲田 のりこ, PARK Pyoyun, IKEDA KENICHI

  日本植物病理学会関西部会, 2012, Japanese, Domestic conference

  Oral presentation


• いもち病菌比病原力遺伝子のMultiple translocationはなぜ起こったか―欠失／再獲得仮説

  CHUMA IZUMI, 古田純一, 草場基章, 吉田健太郎, 寺内良平, 藤田佳克, NAKAYASHIKI HITOSHI, Barbara Valent, TOSA YUKIO

  平成24年度日本植物病理学会大会, 2012, Japanese, Domestic conference

  Oral presentation


• 細胞壁加水分解酵素群はイネ科植物いもち病菌による角皮侵入に寄与している

  伊藤賢司, Quoc Bao Nguyen, TOSA YUKIO, NAKAYASHIKI HITOSHI

  平成23年度日本植物病理学会大会, 2011, Japanese, Domestic conference

  Oral presentation


• いもち病菌の非病原力遺伝子座Avr1-CO39における機能分子の同定

  藤田憲太郎, Hau, V.T.B, Nguyen, Q.B, NAKAYASHIKI HITOSHI, TOSA YUKIO

  平成23年度日本植物病理学会大会, 2011, Japanese, Domestic conference

  Oral presentation


• Building Blocks法によるイネ科植物いもち病菌細胞壁加水分解酵素群の機能解析

  伊藤賢司, Quoc Bao Nguyen, 河南裕美, 村部知里, TOSA YUKIO, NAKAYASHIKI HITOSHI

  平成23年度日本植物病理学会関西部会, 2011, Japanese, Domestic conference

  Oral presentation


• イネ科植物いもち病菌集団における非病原力遺伝子AVR-Pitaの染色体彷徨

  CHUMA IZUMI, 磯部千尋, 堀田祐麻, 茨木加奈, 二俣翔, 草場基章, 吉田健太郎, 寺内良平, 藤田佳克, NAKAYASHIKI HITOSHI, Barbara Valent, TOSA YUKIO

  ムギ類研究会, Nov. 2010, Japanese, 帯広, Domestic conference

  Oral presentation


• ハイドロフォビンMhp1のいもち病菌宿主接着に及ぼす影響

  北岡 治正, 井上 加奈子, NAKAYASHIKI HITOSHI, IKEDA KENICHI, PARK Pyoyun

  日本植物病理学会大会, 2010, Japanese, Domestic conference

  Poster presentation


• RNA silencing as a novel tool for uncovering gene function in fungi

  Nguyen, B.Q, Itoh, K, TOSA YUKIO, NAKAYASHIKI HITOSHI

  The 4th CeFP International Symposium on Fungal Biology and Pathogenesis, 2010, English, Seoul National University, Korea, International conference

  [Invited]

  Invited oral presentation


• 冠さび病菌感染エンバク葉に誘導されるアベナスラミド蓄積とその生合成遺伝子発現の局在と配向解析

  内橋幸平, 岡村薫, 石原亨, PARK Pyoyun, NAKAYASHIKI HITOSHI, TOSA YUKIO, MAYAMA SHIGEYUKI

  平成21年度日本植物病理学会大会, 2009, Japanese, Domestic conference

  Oral presentation


• いもち病菌小分生子の細胞学的特徴

  CHUMA IZUMI, NAKAYASHIKI HITOSHI, TOSA YUKIO

  平成21年度日本植物病理学会関西部会, 2009, Japanese, Domestic conference

  Oral presentation


• いもち病菌におけるハイドロフォビンの接着性と病原性に及ぼす影響

  井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, PARK Pyoyun

  日本植物病理学会大会, 2009, Japanese, Domestic conference

  Poster presentation


• Pathological roles of reactive oxygen species in plant pathogen

  Hyon GS, Morita Y, Kusaka M, Hosogi N, IKEDA KENICHI, Nakayashiki Hitoshi, Park Pyoyun

  6th International Symposium on Electron Microscopy in Medicine and Biology 2009, 2009, English, International conference

  Oral presentation


• ナシ黒斑病菌における活性酸素種 (reactive oxygen species: ROS)生成遺伝子noxAおよびnoxB変異株の細胞学・病理学的比較調査

  玄 康洙, 森田 雄一, IKEDA KENICHI, NAKAYASHIKI HITOSHI, PARK Pyoyun

  日本植物病理学会大会, 2008, Japanese, Domestic conference

  Oral presentation


• いもち病菌におけるハイドロフォビン(Mpg1)の接着能力への影響

  井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, PARK Pyoyun

  第8回糸状菌分子生物学コンファレンス, 2008, Japanese, Domestic conference

  Poster presentation


• Pathological roles of reactive oxygen species at infection structures of Alternaria alternata Japanese pear pathotype during plant infection

  玄 康洙, 森田 雄一, IKEDA KENICHI, NAKAYASHIKI HITOSHI, PARK Pyoyun

  第1回神戸大学バイオサイエンス研究会・若手研究者交流会, 2008, Japanese, Domestic conference

  Poster presentation


• ナシ黒斑病菌の貫穿菌糸における活性酸素種（ROS）生成遺伝子NoxBの機能解析

  玄 康洙, 森田 雄一, 篠木 武, IKEDA KENICHI, NAKAYASHIKI HITOSHI, PARK Pyoyun

  日本植物病理学会関西部会, 2007, Japanese, Domestic conference

  Oral presentation


• ナシ黒斑病菌の貫穿菌糸における活性酸素種（ROS）生成遺伝子NoxAの機能解析

  玄 康洙, 篠木 武, IKEDA KENICHI, NAKAYASHIKI HITOSHI, PARK Pyoyun

  日本植物病理学会, 2007, Japanese, Domestic conference

  Oral presentation


• いもち病菌小型分生子の病理学的役割を解明するための基礎調査

  細木 直樹, CHUMA IZUMI, 篠木 武, IKEDA KENICHI, NAKAYASHIKI HITOSHI, MAYAMA SHIGEYUKI, TOSA YUKIO, PARK Pyoyun

  日本植物病理学会, 2007, Japanese, Domestic conference

  Oral presentation


• いもち病菌のシトシンDNAメチル化は同菌の生活環において必須ではない

  IKEDA KENICHI, 田中 正起, 村田 聡樹, 椎名 宏太, TOSA YUKIO, MAYAMA SHIGEYUKI, PARK Pyoyun, NAKAYASHIKI HITOSHI

  日本植物病理学会関西部会, 2007, Japanese, Domestic conference

  Oral presentation


• ナシ黒斑病菌の貫穿菌糸における活性酸素種（ROS）の機能解析

  玄 康洙, IKEDA KENICHI, NAKAYASHIKI HITOSHI, PARK Pyoyun

  第6回糸状菌分子生物学コンファレンス, 2006, Japanese, Domestic conference

  Poster presentation


• Applications of RNA silencing in Magnaporthe oryzae.

  NAKAYASHIKI Hitoshi

  Plant & Animal Genome XIV Conference. WorkShop AbstactP20., 2006, English, 未記入, San Diego, International conference

  Others


• 比較マッピングによるいもち病菌の染色体構造解析

  茨木 加奈, 中馬 いずみ, 土佐 幸雄, 中屋敷 均, 眞山 滋志

  平成17年日本植物病理学会関西部会講演要旨集p29, 2005, Japanese, 日本植物病理学会, 名古屋市, Domestic conference

  Oral presentation


• 植物病原糸状菌Magnaporthe oryzaeにおけるRNAi経路には二つのDicer 様タンパク質のうち一つが主に関与する

  角谷 直樹, 中屋敷 均, 土佐 幸雄, 眞山 滋志

  平成17年日本植物病理学会大会 （日本植物病理学会報，71: 199）, 2005, Japanese, 日本植物病理学会, 静岡, Domestic conference

  Oral presentation


• トマト転写因子Pti5 遺伝子のプロモーター配列解析

  中屋敷 均, 津田 貴司, G.B. Martin

  平成17年日本植物病理学会大会 （日本植物病理学会報，71: 299-300）, 2005, Japanese, 日本植物病理学会, 静岡, Domestic conference

  Oral presentation


• トマト転写因子Pti4, 5, 6 によるPti5 プロモーターの複合的な制御

  藤井 達也, 津田 貴司, 中屋敷 均, G.B. Martin

  平成17年日本植物病理学会大会 （日本植物病理学会報，71: 300）, 2005, Japanese, 日本植物病理学会, 静岡, Domestic conference

  Oral presentation


• シコクビエいもち病菌集団における非病原力遺伝子PWL1 の解析

  田中 正起, 中屋敷 均, 土佐 幸雄, 眞山 滋志

  平成17年日本植物病理学会大会 （日本植物病理学会報，71: 198-199）, 2005, Japanese, 日本植物病理学会, 静岡, Domestic conference

  Oral presentation


• コムギのエンバクいもち病菌に対する抵抗性遺伝子Rwt4の野外における役割

  Hau V.T.B, 平田 健治, 村上 二朗, 中屋敷 均, 土佐 幸雄, 眞山 滋志

  平成17年日本植物病理学会大会 （日本植物病理学会報，71: 199）, 2005, Japanese, 日本植物病理学会, 静岡, Domestic conference

  Oral presentation


• いもち病菌集団における非病原力遺伝子Avr-Pita 座の変動機構

  中馬 いずみ, 磯部 千尋, 土佐 幸雄, 中屋敷 均, 眞山 滋志

  平成17年日本植物病理学会大会 （日本植物病理学会報，71: 198）, 2005, Japanese, 日本植物病理学会, 静岡, Domestic conference

  Oral presentation


• いもち病菌の種特異的寄生性に関するPWT3 に対応する抵抗性遺伝子の検出とgene-forgene関係の証明

  田澤 佳子, 平田 健治, 中屋敷 均, 土佐 幸雄, 眞山 滋志

  平成17年日本植物病理学会関西部会講演要旨集p30, 2005, Japanese, 日本植物病理学会, 名古屋市, Domestic conference

  Oral presentation


• イネ科植物いもち病菌におけるレトロトランスポゾンMAGGYのメチル化遺伝子の同定

  椎名 宏太, 池田 健一, 角谷 直樹, 中屋敷 均, 土佐 幸雄, 眞山 滋志

  平成17年日本植物病理学会関西部会講演要旨集p30, 2005, Japanese, 日本植物病理学会, 名古屋市, Domestic conference

  Oral presentation


• イネ科植物いもち病菌におけるレトロトランスポゾンMAGGYのメチル化遺伝子の同定

  椎名宏太, IKEDA KENICHI, 角谷直樹, NAKAYASHIKI HITOSHI, TOSA YUKIO, MAYAMA SHIGEYUKI

  日本植物病理学会関西部会, 2005, Japanese, Domestic conference

  Oral presentation


• アワいもち病菌のイチゴツナギ亜科植物に対する非病原性の比較解析

  大村 和孝, 田中 健吾, 中屋敷 均, 土佐 幸雄, 眞山 滋志

  平成17年日本植物病理学会関西部会講演要旨集p29, 2005, Japanese, 日本植物病理学会, 名古屋市, Domestic conference

  Oral presentation


• RNA silencing as a tool for exploring gene function in Ascomycete fungi.

  NAKAYASHIKI Hitoshi, HANADA S, QUOC N.B, KADOTANI N, TOSA Yukio, MAYAMA Shigeyuki

  XXIII Fungal Genetics Conference. Abstact p163., 2005, English, 未記入, California, International conference

  Others


• Pyricularia oryzae 近縁種の種分化過程のGCPSRによる解析

  平田 健治, 草場 基章, 中馬 いずみ, 土佐 幸雄, 中屋敷 均, 眞山 滋志

  平成17年日本植物病理学会大会 （日本植物病理学会報，71: 198）, 2005, Japanese, 日本植物病理学会, 静岡, Domestic conference

  Oral presentation


• One of the two Dicer-like proteins in the filamentous fungi Magnaporthe oryzae genome is responsible for hairpin RNA-triggered RNA silencing and related siRNA accumulation.

  KADOTANI N, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

  XXIII Fungal Genetics Conference. Abstact p159., 2005, English, 未記入, California, International conference

  Others


• DNA methyltransferase は転移因子由来siRNAの蓄積を促進する

  角谷 直樹, 椎名 宏太, 池田 健一, 土佐 幸雄, 眞山 滋志, 中屋敷 均

  第7回RNA学会ミーティング（講演要旨集p86), 2005, Japanese, 日本RNA学会, 弘前大学, Domestic conference

  Oral presentation


• DNA methyltransferaseは転移因子由来siRNAの蓄積を促進する

  角谷 直樹, 椎名 宏太, IKEDA KENICHI, TOSA YUKIO, MAYAMA SHIGEYUKI, NAKAYASHIKI HITOSHI

  第7回RNA学会, 2005, Japanese, 弘前大学, Domestic conference

  Oral presentation


• 様々な植物から分離したPyricularia属菌におけるGCPSRに基づいた種の識別

  平田 健治, 大末 淳, 中馬 いずみ, 土佐 幸雄, 中屋敷 均, 眞山 滋志

  平成16年日本植物病理学会大会, 2004, Japanese, 日本植物病理学会, 福岡, Domestic conference

  Oral presentation


• 植物病原糸状菌Magnaporthe oryzaeにおけるRNAi経路には二つのDicer様タンパク質のうち一つが主に関与する

  角谷 直樹, 中屋敷 均, 土佐 幸雄, 眞山 滋志

  第6回RNAミーティング, 2004, Japanese, RNAミーティング, Domestic conference

  Oral presentation


• セントピートグラスに発生したいもち病菌のrDNA-ITS2領域とbeta-tubulin遺伝子の解析における病原性発現物質の解析

  冨家 和典, 森 真理, 中屋敷 均, 土佐 幸雄, 松浦 一穂, 眞山 滋志, 但見 明俊

  平成16年日本植物病理学会大会, 2004, Japanese, 日本植物病理学会, 福岡, Domestic conference

  Oral presentation


• シコクビエいもち病菌(Magnaporthe oryzae)の集団構造の解析

  田中 正起, 中屋敷 均, 土佐 幸雄, 眞山 滋志

  平成16年日本植物病理学会関西部会, 2004, Japanese, 日本植物病理学会, 松山, Domestic conference

  Oral presentation


• コムギ品種とエンバクいもち病菌間における遺伝子対遺伝子関係の証明

  平田 健治, 土佐 幸雄, 中屋敷 均, 眞山 滋志

  平成16年日本植物病理学会関西部会, 2004, Japanese, 日本植物病理学会, 松山, Domestic conference

  Oral presentation


• エンバクの抵抗性誘導におけるアポトーシス機構の解明14）エンバクにおける宿主特異的毒素ビクトリンへの結合因子のビアコアによる探索

  齋藤 隆一郎, 日下 広, 劉 小露, 中屋敷 均, 土佐 幸雄, 眞山 滋志, 多田 安臣

  平成16年日本植物病理学会大会, 2004, Japanese, 日本植物病理学会, 福岡, Domestic conference

  Oral presentation


• エンバクの抵抗性誘導におけるアポトーシス機構の解明13）宿主特異的毒素ビクトリンは感受性品種IowaX469において細胞表面で認識される

  多田 安臣, 日下 広, 松原 孝英, 大浦 康子, 中屋敷 均, 土佐 幸雄, 眞山 滋志

  平成16年日本植物病理学会大会, 2004, Japanese, 日本植物病理学会, 福岡, Domestic conference

  Oral presentation


• いもち病菌集団における染色体構造の多様性と非病原性遺伝子座の変動

  中馬 いずみ, 磯部 千尋, 土佐 幸雄, 中屋敷 均, 眞山 滋志

  平成16年日本植物病理学会大会, 2004, Japanese, 日本植物病理学会, 福岡, Domestic conference

  Oral presentation


• いもち病菌Magnaporthe oryzaeにおけるRNAサイレンシングベクターの作製

  花田 周吾, 角谷 直樹, 中屋敷 均, 土佐 幸雄, 眞山 滋志

  平成16年日本植物病理学会大会, 2004, Japanese, 日本植物病理学会, 福岡, Domestic conference

  Oral presentation


• Degradation of ribosomal RNA associated with apoptotic response of oat cells trigered by the host-selective toxin, victorin.

  HOAT T X, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

  平成16年日本植物病理学会関西部会, 2004, English, 日本植物病理学会, 松山, Domestic conference

  Oral presentation


• Arabidopsisにおける植物成長促進根圏微生物（PGPR)の遺伝子変動のマイクロアレイ解析

  大原 裕美子, 王 艶青, 中屋敷 均, 土佐 幸雄, 眞山 滋志

  平成16年日本植物病理学会大会, 2004, Japanese, 日本植物病理学会, 福岡, Domestic conference

  Oral presentation


• A convergent opposing promoters system to explore genome-wide gene function in the rice blast fungus by an RNA silencing approach.

  QUOC N B, NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

  平成16年日本植物病理学会関西部会, 2004, English, 日本植物病理学会, 松山, Domestic conference

  Oral presentation


• いもち病菌Magnaporthe oryzae におけるRNA サイレンシング

  角谷 直樹, 中屋敷 均, 土佐 幸雄, 眞山 滋志

  第3回糸状菌分子生物学コンファレンス, 講演要旨集, Nov. 2003, Japanese, 糸状菌分子生物学研究会, 東京大学, Domestic conference

  Oral presentation


• Pyricularia 属集団における染色体構造の多様性

  中馬 いづみ, 磯部 千尋, 土佐 幸雄, 中屋敷 均, 眞山 滋志

  第3回糸状菌分子生物学コンファレンス, 講演要旨集, Nov. 2003, Japanese, 糸状菌分子生物学研究会, 東京大学, Domestic conference

  Oral presentation


• いもち病菌集団における染色体構造の多様性

  中屋敷 均

  第75回日本遺伝大会・ワークショップ講演, 講演要旨集, Sep. 2003, Japanese, 日本遺伝学会, 東北大学, Domestic conference

  Oral presentation


• いもち病菌における過剰染色体の遺伝様式と染色体構造変異の機構

  中馬 いづみ, 土佐 幸雄, 多賀 正節, 中屋敷 均, 眞山 滋志

  第75回日本遺伝大会, 講演要旨集, Sep. 2003, Japanese, 日本遺伝大会, 東北大学, Domestic conference

  Oral presentation


• ペレニアルライグラスいもち病菌の地理的機嫌の解明とコムギに対する発病ポテンシャルの評価

  中川 志織, 土佐 幸雄, 平田 健治, 中馬 いづみ, 磯部 千尋, 中屋敷 均, 森 直樹, WAKAR Uddin, 眞山 滋志

  平成15年日本植物病理学会大会，日本植物病理学会報, Apr. 2003, Japanese, 日本植物病理学会, 明治大学, Domestic conference

  Oral presentation


• ナス科植物青枯病生物防除因子Pseudomonas fluorescens のNOR遺伝子破壊による改変

  王 艶青, 中屋敷 均, 土佐 幸雄, 眞山 滋志

  平成15年日本植物病理学会大会，日本植物病理学会報, Apr. 2003, Japanese, 日本植物病理学会, 明治大学, Domestic conference

  Oral presentation


• いもち病菌集団における染色体構造の多様性

  磯部 千尋, 中馬 いづみ, 土佐 幸雄, 中屋敷 均, 眞山 滋志

  平成15年日本植物病理学会大会，日本植物病理学会報, Apr. 2003, Japanese, 日本植物病理学会, 明治大学, Domestic conference

  Oral presentation


• いもち病菌の種特異的寄生性機構に関する研究(13) いもち病菌雑種後代で認められるadult resistance に対する病原性の低下について

  村上 二朗, 中屋敷 均, 土佐 幸雄, 眞山 滋志

  平成15年日本植物病理学会大会，日本植物病理学会報, Apr. 2003, Japanese, 日本植物病理学会, 明治大学, Domestic conference

  Oral presentation


• いもち病菌における病原性発現物質の解析7)エンバクいもち病菌の生産する新規毒素の白化,活性酸素生成, 細胞死の誘導

  南 友可里, 鶴嶋 鉄, 坂本 勝, 宮川 恒, 中屋敷 均, 土佐 幸雄, 眞山 滋志

  平成15年日本植物病理学会大会，日本植物病理学会報, Apr. 2003, Japanese, 日本植物病理学会, 明治大学, Domestic conference

  Oral presentation


• いもち病菌におけるsupernumerary chromosome の遺伝様式と構造変異機構

  中馬 いづみ, 土佐 幸雄, 多賀 正節, 中屋敷 均, 眞山 滋志

  平成15年日本植物病理学会大会，日本植物病理学会報, Apr. 2003, Japanese, 日本植物病理学会, 明治大学, Domestic conference

  Oral presentation


• GCPSR に基づいたMagnaporthe/Pyricularia 属菌集団における種の識別

  平田 健治, 大末 淳, 中馬 いづみ, 土佐 幸雄, 中屋敷 均, 眞山 滋志

  平成15年日本植物病理学会大会，日本植物病理学会報, Apr. 2003, Japanese, 日本植物病理学会, 明治大学, Domestic conference

  Oral presentation


• いもち病菌Magnaporthe grisea におけるsmall interfering RNAs (siRNAs)の検出

  角谷 直樹, 中屋敷 均, 土佐 幸雄, 眞山 滋志

  平成15年日本植物病理学会大会，日本植物病理学会報, 2003, Japanese, 日本植物病理学会, 未記入, Domestic conference

  Oral presentation


• Mechanisms of species-specific parasitism in Magnaporthe oryzae.

  TOSA Yukio, NAKAYASHIKI Hitoshi, MAYAMA Shigeyuki

  Plant & Animal Genome XI Conference., 2003, English, 未記入, 未記入, International conference

  Oral presentation


• Apoptotic response of oat leaf cells in defense against infection.

  MAYAMA Shigeyuki, NAN Y, KUSAKA K, TADA Y, YANG Q, SHIGEMI T, IMAI S, NAKAYASHIKI Hitoshi, TOSA Yukio, PARK P

  9th US-Japan Seminar on Plant-pathogen Interactions., 2003, English, 未記入, 未記入, International conference

  Oral presentation

• 日本遺伝学会


• 植物病理学会

• 植物病原糸状菌の寄生性を規定するクロマチン状態の解明

  中屋敷 均

  日本学術振興会, 科学研究費助成事業, 基盤研究(B), 神戸大学, 01 Apr. 2021 - 31 Mar. 2024


• Comprehensive analysis of small RNAs produced by the blast fungus, Pyricularia oryzae

  Nakayashiki Hitoshi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), Kobe University, 30 Jul. 2020 - 31 Mar. 2022

  In this study, a novel class of small RNAs (sRNAs) was identified in the blast fungus, Pyricularia oryzae. The novel sRNAs were characterized by its smaller size (16-18nt), Dicer- and/or RdRP-independent biogenesis, and nonbinding to AGO protien. Northern blotting analysis revealed several sizes of precursors of the novel sRNA, indicating multiple steps of processing for its biogenesis. Consistently, RNA-seq analysis suggested that some of the precursors were generated via complex splicing. GFP reporter assay indicated that the novel sRNA was not functional in the gene silencing pathways, supporting the idea that these sRNAs possess so-far unknown biological roles.


• 生物間相互作用に介在する小分子RNAの進化機構

  中屋敷 均

  学術研究助成基金助成金／挑戦的研究（萌芽）, Apr. 2017 - Mar. 2019, Principal investigator

  Competitive research funding


• 糸状菌におけるクロマチン修飾を介した病原遺伝子制御カスケードの包括的理解

  中屋敷 均

  科学研究費補助金／基盤研究(B), Apr. 2016 - Mar. 2019, Principal investigator

  Competitive research funding


• Antiviral innate immunity in phytopathogenic fungi

  SUZUKI Nobuhiro

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Okayama University, 01 Apr. 2013 - 31 Mar. 2017

  The objective of this project is to explore RNA silencing (RNAi) as the antiviral innate defense against fungal viruses in plant pathogenic filamentous fungi. Consequently, a great progress has been made, leading to breakthroughs: 1) Discovery of RNAi mediated antiviral defense that requires Dicer but not AGO in Cryphonectria parasitica, 2) Detection of inhibitory effects on RNA silencing activities against RNA and DNA viruses, and transgenes in Pyricularia oryzae, 3) Development of a screening protocol for host factors required for virus perception through transcription induction of key genes of RNAi upon virus infection in C. parasitica, 4) Identification of the SAGA complex as a transcriptional regulator of fungal RNA silencing. These findings will bring about a paradigm shift in RNAi. Fungi are now established as the third position next to animals and plants in antiviral defense research. Note that most of the obtained data were published in international, high-quality journals.


• いもち病菌の感染器官形成におけるクロマチン修飾のダイナミズム

  中屋敷 均

  科学研究費一部基金／基盤研究(B)特設, Apr. 2013 - Mar. 2016, Principal investigator

  Competitive research funding


• 持続可能な農業生産のための新たな総合的植物保護技術の開発

  中屋敷 均

  戦略的イノベーション創造プログラム（SIP）, 2016, Principal investigator

  Competitive research funding


• Ｂｕｉｌｄｉｎｇ ｂｌｏｃｋｓ法による高効率多重遺伝子サイレンシング系の確立

  中屋敷 均

  科学研究費補助金／萌芽研究, Apr. 2012 - Mar. 2014, Principal investigator

  Competitive research funding


• イネ科植物いもち病菌の病原性に果たすクロマチンリモデリング遺伝子の役割

  中屋敷 均

  科学研究費補助金／基盤研究(B), 2010, Principal investigator

  Competitive research funding


• Establishment of disease control method for plant pathogenic fungi by the detachment of the spore germlings on the plant surface

  IKEDA Kenichi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Kobe University, 2007 - 2008

  本研究では、いもち病菌の宿主葉面接着能力に着目し、病原菌の接着に関わる分子機構の解明と、宿主接着を阻害することによる新たな病害防除法の可能性について検討した。病原菌の宿主接着はマンノース含有糖タンパク質を含む複合構造により発揮されることが明らかとなった。また、この強固な接着はコラゲナーゼ等の糖タンパク質分解酵素で剥離可能であった。さらに、病原菌を剥離させる能力を有する微生物を自然界より選抜した。


• ハイスループットＲＮＡｉ法によるイネいもち病菌のゲノム機能解析

  中屋敷 均

  特別研究員奨励費, 2008, Principal investigator

  Competitive research funding


• イネいもち病菌における非病原力ＲＮＡの探索

  中屋敷 均

  科学研究費補助金／萌芽研究, 2007, Principal investigator

  Competitive research funding


• 植物病害における病原性因子の役割に関する電子顕微鏡・生化学解析

  朴 杓允

  科学研究費補助金／基盤研究(B), 2006

  Competitive research funding


• ＲＮＡサイレンシングによるイネいもち病菌のゲノムワイドな病原性遺伝子解析

  中屋敷 均

  科学研究費補助金／基盤研究(B), 2005, Principal investigator

  Competitive research funding


• Ｄｉｃｅｒ蛋白質の機能ドメイン分析とその機能分化の進化的解析

  中屋敷 均

  科学研究費補助金／特定領域研究, 2005, Principal investigator

  Competitive research funding


• いもち病菌染色体における非病原性遺伝子アイランドの同定とその転移・分解機構の解析

  土佐 幸雄

  科学研究費補助金／基盤研究(B), 2005

  Competitive research funding


• Identification and cloning of genes conditioning species-specific parasitism of Magnaporthe oryzae

  MAYAMA Shigeyuki, TOSA Yukio, NAKAYASHIKI Hitoshi, PYOYUN Park

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Kobe University, 2002 - 2004

  To clarify genetic mechanisms of plant-species specificity of Magnaporthe oryzae, we crossed a Triticum isolate with a Setaria isolate, an Avena isolate, and an Oryza isolate. Using the F_1 progenies derived from these crosses, we identified PWT1, PWT2 (conditioning avirulence of the Setaria isolate on wheat), PWT3, PWT4 (conditioning avirulence of the Avena isolate on wheat), PWT5 (conditioning avirulence of the Oryza isolate on wheat), PFM1, PFM2 (conditioning avirulence of the Triticum isolate on foxtail millet), and PAT1 (conditioning avirulence of the Triticum isolate on oat). We also identified a resistance gene corresponding to PWT4, and designated it as Rwt4. Rwt4 corresponded to PWT4 in a one-to-one manner, suggesting that the plant species specificity of the Triticum/Avena isolates on wheat is conditioned by gene-for-gene interactions. From these avirulence genes, PWT1, PWT4, and PAT1 were chosen as targets for molecular cloning. Using bulked segregant analysis, we found molecular markers that are closely linked to PWT1, PWT4 and PAT1.


• Studies on the involvement of transposons in the pathogenicity variations of the rice blast fungus

  NAKAYASHIKI Hitoshi, TOSA Yukio, MAYAMA Shigeyuki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 2001 - 2002

  1. Deletion analysis of the MAGGYLTR promoter Several constructs having a deletion in the MAGGY promoter were made and examined for the promoter activity upon stress using the GUS gene as a reporter. The deletion constructs with the GUS gene were introduced into M.grisea isolate Br 48. Total protein was extracted from the M.grisea transformants after beat shock treatment (42C, 1hr), and the GUS activity was measured using MUG as a substrate. The construct that lacks up to 60 bp at 5' terminus of LTR retained the promoter activity and responded to heat shock whereas one lacking 90 bp at 5' terminus lost the promoter activity. The results indicated that some critical sepuence required for both promoter activity and stress response occur in the 60 to 90 region of MAGGYLTR. 2. Analysis of the promoter activity of MAGGY during infection processes using the GFP gene To investigate the promoter activity of MAGGY during infection processes, GFP was expressed in a M.grisea transformant under the control of the MAGGY promoter. Infection test was performed with the GUS-expressing transformants. However, no obvious upregulation of the MAGGY promoter was found during infection processes on either resistant or susceptible cultivar of rice. 3. Isolation of a new LTR-retrotransposn from M.grisea. As a new candidate for a stress responsive transposable element, an LTR-retrotransposon, Pyret was isolated from M.greisea. Pyret containg gag and pol genes as do ordinary gypsy-class retrotransposons. However, this element is unique since it carries an extra domain in the gag gene, which is designated as WCCH domain according to a characteristic amino acid sequence. The WCCH domain was found only in four gypsy-class retrotransposons identified in fungal genomes so far. Therefore, the functional role of the WCCH domain is unknown at this moment.


• Cooperative research on biologicalcontrolofplantpest

  MAYAMA Shigeyuki, NAKAYASHIKI Hitoshi, PARK Pyoyun, TOSA Yukio, MIZUNO Masashi, TAKEDA Makio

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Kobe University, 1999 - 2001

  Current status in China of biological control of plant diseases and insect pest was studied. The recent development of biological research and application was summarized in the final report. We especially studied on biological control of tomato bacterial wilt by PGPR strain, Pseaudomonas fluorescence FPT9601. It was found that the strain could colonize within the root tissues of both monocot and dicot plants. They formed microcolonies in a concave portion in the root surface such as injured places from which bacterial pathogens can be easily invaded, indicating that the presence of PGPR strains in the portion seem to be appropriate for suppressing the pathogens. Once the bacteria were invaded in roots, an induced resistance was activated in plants. Flaggellin components of the bacteria were important factor for colonization and induction of defense response in plants. Also, 2,4-DAPG, diacetylphlorogrucinol, was found as a major antibacterial factor to suppress the bacteria wilt strain. On the other hand, the methods employed for biological control of insect pests have been studied in China. It was found that introduction of natural enemies, conservation, environment management for manipulation of natural enemies, augmentation and release of biological control agents have been developed and they greatly contribute to the improvement of the biological control in China.


• Molecular analysis of species-specific parasitism in Magnaporthe grisea

  TOSA Yukio, PARK Pyoyun, NAKAYASHIKI Hitoshi, MAYAMA Shigeyuki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B)., KOBE UNIVERSITY, 1998 - 2000

  Mechanisms of the species-specific parasitism in Magnaporthe grisea were investigated. An F1 population derived from a cross, a Setaria isolate x a Tritucum isolates, showed bifactorial segregation in the pathogenicity on wheat. These genes were designated as Pwt1 and Pwt2. Another F1 population derived from a cross, an Avena isolate and a Triticum isolate, showed monofactorial segregation on oat. This gene was designated as Pat1. Closely linked AFLP markers were found in bulked segregant analyses. Chromosome walking is under way toward cloning of those genes. A phytotoxin was extracted from a Digitaria isolate. The toxin was revealed to be Pyrichalasin H.Among various gramineous plants, crabgrass (the host of Digtaria isolates) showed the highest susceptibility to this toxin. Pyrichalasin H was produced in a large quantity only by Digitaria isolates, and Pyrichalasin H-defidient mutants lost their pathogenicity on crabgrass. Crabgrass leaves treated with Pyrichalasin H became accessible to nonpathogens. These results suggested that Pyrichalasis H is a determinant of the species-specific parasitism of Digitaria isolates. Retrotransposon MAGGY was the most active element among transposable elements that have been found in the genome of Magnaporthe grisea. MAGGY was present in Oryza isolates and Setaria isolates, but absent in other isolates. We assuned that this element may have been involved in the evolution of the race- cultivar specificity in those isolates.


• イネいもち病菌の病原性変異機構の解析−特に転移因子の関与について

  中屋敷 均, 土佐 幸雄, 眞山 滋志

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 1998 - 1999

  イネいもち病菌に存在するレトロトランスポゾンMAGGYの、同菌の病原性変異への関与を調査する目的で、今年度は二項目の研究を行い以下に記した結果を得た。 1. MAGGYの活性化条件を調べるために、MAGGYのプロモーターが存在するLTR配列の下流にレポーターとしてGUS遺伝子を結合したプラスミドを作製した。このプラスミドをPEG法によりイネいもち菌に導入して、ストレスによるMAGGYの活性化を検討した。その結果、MAGGYのプロモーター活性は、42℃、1時間の熱ショックにより、約60倍増高することが明らかになった。また、500mM過酸化水素処理によっても約20倍の増高が認められた。今後、このコンストラクトを用いることによって、菌の感染行動と関連したMAGGYの活性化についてさらに調査を続ける予定である。 2. 通常感染が成立しないイネ-いもち病菌の組み合わせでも、イネの穂ばらみ期に注射接種することによって低頻度の感染が起こることが報告されている。この条件下で、感染が起こった病斑から単胞子分離を行い、これらの菌系における病原性の変異とMAGGYの転移との相関性を調査した。これまでに約170菌系を単胞子分離により確立し、その内の15菌系において病原性の変化が見られた。現在の所、それと明確に対応するMAGGYの転移バンドは確詔されていないが、今後F1菌系における転移バシドと病原性の分離と連鎖を調査し、更に解祈を進める予定である。


• Rapid identification of races and species-specific pathotypes using DNA-fingerprinting

  MAYAMA Shigeyuki, TOSA Yukio

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 1998 - 1999

  DNA fingerprinting with miltilocus probes, MAGGY and MGR586, were conducted to investigate the population structure of the rice blast fungus, Magnaporthe grisea in Japan. Analyses of ca. 300 isolates revealed that two discrete lineages exist in the current rice blast population in Japan. They were designated as JL1 and JL2. There two lineages corresponded to two of the five lineages previously detected in collections made before 1960, suggesting that the structure of the rice blast population in Japan had drastically changed during 1960-1976. Rice blast population in Vietnam were also subjected to DNA fingerprinting with MAGGY probe and the pathotype analysis. These analyses revealed that the blast population in the northern region of Vietnam is genetically more diverse than that in the southern region. In both populations no relationships were detected between lineages and races, suggesting that the "lineage exclusion hypothesis" is not applicable to those populations. DNA fingerprinting experiments were carried out for establishing a system of DNA-diagnosis of six host-specific subgroups of M. grisea ; Oryza isolates, Triticum isolates, Panicum isolates, Setaria isolates, Eleusine isolates, and Digitaria isolates. We propose the following formulas for distinguishing those subgroups. (1)HindIII+MGR583 : to identify each group (2)SalI+MAGGY : to identify the Oryza and the Setaria isolates (3)EcoRV+MGR583 : to identify the Triticum isolates (4)HindIII+Pot2 : to identify the Digitaria isolates


• Molecular Mechanisms of Resistance Gene Expression in the Crown Rust of Oats

  MAYAMA Shigeyuki, MIZUNO Masashi, NANMORI Takashi, NAKAYASHIKI Hitoshi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 1996 - 1997

  The purpose of this research project is to elucidate the molecular mechanisms of recognition and signal transduction which regulate disease occurence in oats and crown rust interactions. Special reference was concerned with (1) a role of release of activated oxygen, (2) cloning of genes activated during resistance expression, such as chitinase as PR protein and genes responsible to phytoalexin production and signal transduction. As a result, it was found that evolvement of activated oxygen is absolutely required for initiation of genes for phytoalexin production. The cells affected by infection as well as elicitors reacted positively to stains which detect free radicals including activated oxygen. Activated oxygen could be important for induction of resistance as a secondary messengers and induce gene activation for PR protein, phytoalexin and so on. Chitinase was induced in parallel with resistance expression. The enzymes were purified and N-teminal sequence of amino acid was determined and the genes encoded with chitinase have been cloned. In addition, it was found that the chitinases can release chito-oligosaccharides active as elicitor. Finally, it was found that DNA-laddering as a typical symptom of apoptosis was detected in association of resistance response in oat cells treated with rust races and elicitors. Relationships between apoptosis and defense response was confirmed.


• いもち病菌の病原性、寄生性分化と連鎖するDNAマーカー配列の検索

  中屋敷 均

  日本学術振興会, 科学研究費助成事業, 奨励研究(A), 神戸大学, 1994 - 1994

  いもち病菌には各種イネ科植物、あるいは品種に特異的に病原性を持つ種分化やレース分化が起きており、これらの菌は病原性、交配能、小分生子形成能等に異なった生物学的特徴を持っている。これらの生物学的な特徴と連鎖し、それらを担った遺伝子のマーカーとなるDNA配列を検索することを目的とした。本年度はその第一歩としてRFLP分析を行い、それによるいもち菌の系統発生的な解析を行った。 イネ科栽培植物および雑草に寄生性を示す43種の各種いもち病菌から抽出したDNAを、22種のプローブ、2種の制限酵素を用いてRFLP分析した結果、各菌はミョウガ菌群、メヒシバ菌群、栽培植物菌群に大別され、さらに栽培植物菌群は、大きくコムギ・シコクビエ菌群とアワ菌・キビ菌・イネ菌群の2つに分かれることが明らかとなった。各種雑草菌では、ハルガヤ、クサヨシ、ネズミムギ、ヒロハノウシノケグサ等の菌がイネ菌に、ハイキビ菌がキビ菌に、エノコログサ菌がアワ菌に、またトウジンビエ、エゾノサヤヌカグサ、ブッフェルグラス菌等がメヒシバ菌に、それぞれ近縁な菌であることが明らかとなり、雑草菌が新たな菌群を単独で形成することはなかった。これらの結果は、これまでに分かっている各菌の病原性の分化によるグル-ピングとよく一致しており、DNAを用いた病原性遺伝子へのアプローチが有効な方法であることが示された。