Research activity information

• Nov. 2019 肉用牛研究会, 肉用牛研究会賞


• Oct. 2019 神戸大学, 学長表彰（財務貢献者）


• Sep. 2018 日本動物遺伝育種学会, 日本動物遺伝育種学会優秀発表賞, 兵庫県黒毛和種における全ゲノムリシーケンスデータを用いた枝肉形質に対する候補変異の抽出

  木越洸利, 川口芙岐, 大山憲二, 万年英之, 笹崎晋史

  Japan society


• Mar. 2018 日本畜産学会, 第124回日本畜産学会大会優秀発表賞, ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索

  川口芙岐, 木越洸利, 小浜菜美子, 秋山敬孝, 福島護之, 吉田恵実, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

  Japan society


• Sep. 2017 一般社団法人産業応用工学会, Best Paper Award, Estimation of Calf Weight from Fixed-Point Stereo Camera Images Using Three-Dimensional Successive Cylindrical Model

  山下 歩, OHKAWA TAKENAO, 大山 憲二, 太田 能, 西出 亮, 本多 健

  International society


• Nov. 2016 日本動物遺伝育種学会, 第17回日本動物遺伝育種学会大会・学会長特別賞, 黒毛和種繁殖雌牛集団におけるレプチン遺伝子内多型と繁殖形質との関連

  川口芙岐, 深澤謙治, 小浜菜美子, 秋山敬孝, 福島護之, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

  Japan society


• Aug. 2016 Secretariat for AAAP2016, 17th AAAP Animal Science Congress Young Scientists Award, Estimation of genetic parameters for stillbirth of Japanese Black cattle in Kagoshima

  Oyama H, Nishi K, Imamura K, Sakamoto S, Kawabe K, Okamoto S, HONDA TAKESHI, OYAMA KENJI, Shimogiri T

  International society


• Mar. 2015 Japanese Society of Animal Science, Animal Science Journal Excellent Paper award, Effect of DNA polymorphisms related to fatty acid composition in adipose tissue

  Narukami T, Sasazaki S, Oyama K, Nogi T, Taniguchi M, Mannen H

  Japan society


• Mar. 2013 Japanese Society of Animal Science, Animal Science Journal Excellent paper award, Effect of SCD and SREBP genotypes on fatty acid composition in adipose tissue of Japanese Black cattle herds

  Ohsaki H, Tanaka A, Hoashi S, Sasazaki S, Oyama K, Taniguchi M, Mukai F, Mannen H

  Japan society

• Comprehensive genotyping analysis of single nucleotide polymorphisms responsible for beef marbling in Japanese Black cattle

  Shinji Sasazaki, Hina Kondo, Yurika Moriishi, Fuki Kawaguchi, Kenji Oyama, Hideyuki Mannen

  Abstract Background Beef marbling is considered a desirable trait in the meat industry. Therefore, understanding the genetic factors that cause marbling is important. Previously, we performed a genome-wide association study to examine genetic factors associated with beef marbling in Japanese Black cattle and identified a candidate region between 10–30 Mbp on chromosome 7. We verified the effect of the SNPs in this region on beef marbling using linkage disequilibrium block analysis. We narrowed down the candidate region to a range of 15.8–16.1 Mbp. In this study, we comprehensively detected all of the SNPs in this region and verified their effects on beef marbling. Results Genome resequencing using four animals exhibiting high beef marbling standard (BMS) and four with low BMS revealed a total of 1,846 polymorphisms within the candidate region. Based on the annotation, we selected 13 SNPs exhibiting a moderate impact, as no high-impact SNPs were detected. All of the SNPs represented missense polymorphisms and were located in the following seven genes: RDH8, ANGPTL6, DNMT1, MRPL4, ICAM1, ICAM3, and ICAM5. Finally, we determined the effects of these SNPs on the BMS of a Japanese Black cattle population (n = 529). Analysis of variance revealed that the five SNPs were located in genes encoding the intercellular adhesion molecules (ICAM1, ICAM3, and ICAM5), and showed a highly significant association compared with the remainder (p < 0.01). The lowest p-value was observed for ICAM3_c.739G > A (p = 1.18E-04). Previous studies have suggested that intercellular adhesion molecules (ICAM) may be an upstream factor that regulates adipocyte differentiation. Therefore, considering the polymorphism and putative gene function, we suggest that ICAM1 is potentially responsible for beef marbling. c.470C > G and/or c.994G > A on ICAM1 may be responsible for this quantitative trait locus. Conclusions Promising SNP candidates responsible for beef marbling were identified using extensive polymorphism verification in a previously reported QTL region. We aim to elucidate the mechanism of beef marbling in future studies by investigating how these polymorphisms alter protein structure and function.

  Springer Science and Business Media LLC, Feb. 2024, BMC Genomic Data, 25(1) (1)

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Estimation of genetic parameters for visceral diseases of fattening Japanese Black cattle in Shimane Prefecture, Japan.

  Ryoichi Nakamura, Makiko Sasaki, Nobutaka Shoji, Masaru Takahashi, Akihiro Watanabe, Kumi Iijima, Kiyotoshi Hasegawa, Kenji Oyama, Hideyuki Mannen

  In this study, the genetic parameters of major visceral diseases were estimated using the postmortem inspection records of 9057 fattening Japanese Black cattle in Shimane Prefecture, Japan, and the genetic correlation between visceral diseases and carcass traits was analyzed. There were six visceral diseases with a prevalence of 5% or higher, namely, pleurisy, pneumonia, bovine abdominal fat necrosis (BFN), rumenitis, hemorrhagic hepatitis, and perihepatitis. Variance components were estimated using the Gibbs sampling method, and the heritability of the visceral disease ranged from 0.07 to 0.49 for perihepatitis and BFN, respectively. Significant negative genetic correlations were identified between pleurisy and rib thickness (-0.32), BFN and carcass weight (-0.29), and BFN and rib eye area (-0.22). No significant genetic correlation was observed among the visceral diseases. The least squares analysis of variance suggested that some visceral diseases decrease the value of carcass traits. In particular, carcass weight and rib eye area in individuals with BFN were 11.7 kg and 1.87 cm2 lower than those of healthy cattle, respectively. Thus, it was inferred that genetic factors were involved in the visceral diseases of fattening Japanese Black cattle in Shimane Prefecture.

  2024, Animal science journal = Nihon chikusan Gakkaiho, 95(1) (1), e13930, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• SCIRNet: Skeleton-based cattle interaction recognition network with inter-body graph and semantic priority.

  Yang Yang, Mizuka Komatsu, Kenji Oyama, Takenao Ohkawa

  IEEE, Jun. 2023, IJCNN, 1 - 8, English

  [Refereed]

  International conference proceedings


• Verification of Candidate SNP Effects Reveals Two QTLs on BTA7 for Beef Marbling in Two Japanese Black Cattle Populations

  Shinji Sasazaki, Raito Yamamoto, Shintaro Toyomoto, Hina Kondo, Takayuki Akiyama, Namiko Kohama, Emi Yoshida, Fuki Kawaguchi, Kenji Oyama, Hideyuki Mannen

  In our previous study, we used genome resequencing to detect all candidate polymorphisms within a quantitative trait loci (QTL) region for beef marbling reported previously at 10–30 Mbp on bovine chromosome 7, and we selected 6044 polymorphisms as candidate quantitative trait nucleotides (QTNs). In the present study, we aimed to identify quantitative trait genes (QTGs) and QTNs in this QTL region by verifying the effect of SNPs on beef marbling in two Japanese Black cattle populations using a Dynamic Array integrated fluidic circuit. In total, 96 selected SNPs were genotyped in 441 and 529 animals in Hyogo and Miyazaki cattle populations, respectively. The most significant p-values were detected in a SNP in a splice region of ALDH7A1 (SNP93_ALDH7A1; p = 3.46 × 10−5) in Hyogo cattle and a missense polymorphism of intercellular adhesion molecule-1 (ICAM1) (SNP37_ICAM1; p = 3.33 × 10−4) in Miyazaki cattle. Interestingly, SNP93_ALDH7A1 was not significant (p = 0.459) in Miyazaki cattle, and SNP37_ICAM1 showed a weakly significant association (p = 0.043) in Hyogo cattle. Thus, each population would likely have different QTGs and QTNs for beef marbling in the QTL region. In the Hyogo population, it was not possible to determine the accurate range of the linkage disequilibrium (LD) block in LD block analysis because of a strong LD structure throughout the assessed region. In Miyazaki cattle, however, an LD block containing SNP37_ICAM1 had a range of 15.8–16.1 Mbp, suggesting that QTNs would be located within this region. The functions of 19 genes in the LD block were investigated. ICAM1 is known to play an important role in adipocyte differentiation; given this function and the effect of amino acid substitution, SNP37_ICAM1 was identified as a promising candidate QTN for beef marbling. Further research on the effect of SNP37_ICAM1 on adipocyte differentiation is expected to provide insights into the mechanism underlying beef marbling formation.

  MDPI AG, Jul. 2022, Genes, 13(7) (7), 1190 - 1190

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Real-Time Cattle Interaction Recognition via Triple-stream Network.

  Yang Yang, Mizuka Komatsu, Takenao Ohkawa, Kenji Oyama

  IEEE, 2022, 21st IEEE International Conference on Machine Learning and Applications(ICMLA), 61 - 68

  International conference proceedings


• Estimation of genetic parameters for carcass grading traits, image analysis traits, and monounsaturated fatty acids in Japanese Black cattle from Hyogo Prefecture

  Namiko Kohama, Emi Yoshida, Tatsunori Masaki, Eiji Iwamoto, Moriyuki Fukushima, Takeshi Honda, Kenji Oyama

  Genetic parameters for carcass grading traits, image analysis traits, and monounsaturated fatty acid (MUFA) percentages were estimated in 29,942 Japanese Black cattle from Hyogo Prefecture. The analyzed traits included five carcass grading traits, two image analysis traits, fat area ratio and fineness index, and two MUFA traits, one measured in intermuscular fat using near-infrared spectroscopy (NIRS) and the other in intramuscular fat using gas chromatography (GC). The heritability estimates of image analysis traits and MUFA were moderate to high, ranging from 0.395 to 0.740, and it was considered that they could be improved simultaneously with carcass grading traits because no severe genetic antagonism was observed. Although the heritability of the NIRS-based intermuscular MUFA was slightly lower than that of the GC-based intramuscular MUFA, the genetic correlation between the two methods was as high as 0.804. These results indicate that the NIRS method can be used as an alternative evaluation procedure to predict MUFA in intramuscular fat in the longissimus muscle.

  Corresponding, Wiley, Dec. 2021, Animal Science Journal, 92(1) (1), e13664, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Depth Image Selection Based on Posture for Calf Body Weight Estimation

  Yuki Yamamoto, Takenao Ohkawa, Chikara Ohta, Kenji Oyama, Ryo Nishide

  MDPI, Nov. 2021, The 13th EFITA International Conference

  [Refereed]

  International conference proceedings

  Link to Kobe Univ. Repository (Kernel)


• Effects of carcass traits, proportion of monounsaturated fatty acids and fineness index of marbling on the carcass unit price of Japanese Black cattle in Hyogo prefecture

  Namiko KOHAMA, Takayuki AKIYAMA, Moriyuki HUKUSHIMA, Takeshi HONDA, Kenji OYAMA

  Corresponding, Japanese Society of Animal Science, Nov. 2021, Nihon Chikusan Gakkaiho, 92(4) (4), 511 - 517, Japanese

  [Refereed]

  Scientific journal


• Estimation of genetic parameters for stillbirth of Japanese Black cattle in Kagoshima

  Hidemi OYAMA, Kiyoto IMAMURA, Shinichi SAKAMOTO, Kazutaka NISHI, Kotaro KAWABE, Shin OKAMOTO, Takeshi HONDA, Kenji OYAMA, Takeshi SHIMOGIRI

  Japanese Society of Animal Science, Aug. 2021, Nihon Chikusan Gakkaiho, 92(3) (3), 285 - 291, English

  [Refereed]

  Scientific journal


• Effect of Five Polymorphisms on Percentage of Oleic Acid in Beef and Investigation of Linkage Disequilibrium to Confirm the Locations of Quantitative Trait Loci on BTA19 in Japanese Black Cattle

  Fuki Kawaguchi, Fuka Kakiuchi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Five polymorphisms associated with the percentage of oleic acid (C18:1) in beef fat were previously reported on bovine chromosome 19 in different Japanese Black cattle populations. This study aimed to verify the effects of these five polymorphisms on C18:1 using the same Japanese Black cattle population and conduct linkage disequilibrium (LD) analysis in order to determine the locations of the quantitative trait loci (QTLs). We genotyped the five polymorphisms (SREBP1 c.1065 + 83 (84bp indel), STARD3 c.1187 C > T, GH c.379 C > G, FASN g.841 G > C, and FASN g.16024 A > G) in two populations, which were bred in Hyogo and Gifu Prefectures, Japan (n = 441 and 443, respectively) in order to analyze their effects on C18:1 using analysis of variance (ANOVA). In the Hyogo population, SREBP1 c.1065 + 83 and STARD3 c.1187 C > T were significantly associated with C18:1 (p < 0.001). Meanwhile, FASN g.841 G > C, FASN g.16024 A > G, and GH c.379 C > G were significantly associated with C18:1 (p < 0.01) in the Gifu population. LD analysis was subsequently conducted to detect the range of the QTLs, which ranged from 32.2 to 46.4 Mbp and from 47.8 to 52.1 Mbp in the Hyogo and Gifu populations, respectively. In conclusion, this study confirmed the existence of QTLs on BTA19 and divided the candidate region for each QTL based on LD coefficients. These results could contribute to efficient searches for responsible genes and polymorphisms for fatty acid composition.

  MDPI AG, Jun. 2021, Life, 11(7) (7), 597 - 597

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Error Factor Analysis of DNN-based Fingerprinting Localization through Virtual Space.

  Takuto Jikyo, Tomio Kamada, Chikara Ohta, Takaharu Yaguchi, Kenji Oyama, Takenao Ohkawa, Ryo Nishide

  IEEE, Jan. 2021, 18th IEEE Annual Consumer Communications & Networking Conference(CCNC), 1 - 4

  [Refereed]

  International conference proceedings


• Effect of DNA markers on the fertility traits of Japanese Black cattle for improving beef quantity and quality

  Fuki Kawaguchi, Miyako Tsuchimura, Kenji Oyama, Tamako Matsuhashi, Shin Maruyama, Hideyuki Mannen, Shinji Sasazaki

  Abstract. Carcass traits have been efficiently improved by recent selection using DNA markers in beef cattle. Additionally, DNA markers might have an effect on other traits such as fertility traits; therefore attention should also be paid to such pleiotropic effects. However, the effects of the markers on both carcass and fertility traits have never been evaluated in the same population, since they are generally measured in different populations. The objective in the current study was to discuss effectiveness of DNA markers developed for carcass traits through investigation of their effects on carcass and fertility traits in a population. We genotyped six markers SCD V293A, FASN g.841G>C, PLAG1 g.49066C>G, NCAPG I442M, DGAT1 K232A, and EDG1 g.1471620G>T in a Japanese Black cattle population (n=515). To investigate their effects on carcass and fertility traits, we performed statistical analysis (ANOVA and the Tukey–Kramer honestly significant difference (HSD) test). In the results, three of six markers, SCD V293A, NCAPG I442M, and EGD1 g.1471620G>T, were significantly associated with both carcass and fertility traits. Remarkably, the same allele for each marker had positive effects on both traits, suggesting that we would be able to simultaneously improve them using these markers in this population. However, previous studies reported that the effects of DNA markers could differ among populations. Therefore, it is necessary to confirm the effect of the marker in each population before it is used for improvement.

  Copernicus GmbH, Jan. 2020, Archives Animal Breeding, 63(1) (1), 9 - 17, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Detection of candidate polymorphisms around the QTL for fat area ratio to rib eye area on BTA7 using whole‐genome resequencing in Japanese Black cattle

  Shinji Sasazaki, Fuki Kawaguchi, Ayaka Nakajima, Raito Yamamoto, Takayuki Akiyama, Namiko Kohama, Emi Yoshida, Eiji Kobayashi, Takeshi Honda, Kenji Oyama, Hideyuki Mannen

  Wiley, Jan. 2020, Animal Science Journal, 91(1) (1), e13335

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Estimation of genetic parameters of defective appearances in Japanese Black heifer calves in Kagoshima

  Oyama H, Imamura K, Sakamoto S, Nishi K, Kawabe K, Okamoto S, Honda T, Oyama K, Shimogiri T

  Jan. 2020, Animal Science Journal, 91, e13338

  [Refereed]

  Scientific journal


• The non-synonymous mutation in bovine SPP1 gene influences carcass weight

  Matsumoto H, Kohara R, Sugi M, Usui A, Oyama K, Mannen H, Sasazaki S

  Meat quality in beef cattle is controlled by genetic factors. SPP1 (secreted phosphoprotein 1) gene, coding a multifunctional cytokine with diverse biological functions, is the candidate gene influencing carcass traits. In this study, we tried to discover DNA polymorphisms associated with beef quality in bovine SPP1 gene, so that two SNPs (single nucleotide polymorphisms) in the promoter region and one SNP in the CDS (coding sequence) region were identified. Although the formers were predicted to alter SPP1 expression, they did not show any effects on the traits. On the contrary, statistical analysis revealed that g.58675C > T, a non-synonymous mutation from threonine to methionine in the conservative region, had a significant effect on carcass weight. Carcass weight of the animals with C/T allele (473.9 ± 6.0 kg) was significantly heavier than that of the C/C homozygotes (459.2 ± 2.8 kg). Because SPP1 gene functions in skeletal muscle cells as a positive regulator, the non-synonymous mutation might influence muscle development and remodeling, resulting in increased carcass weight of the C/T animals. Our results indicate that the SNP can be applied as a DNA marker for the improvement of beef cattle.

  Dec. 2019, Heliyon, 5(12) (12), e03006, English, International magazine

  [Refereed]

  Scientific journal


• Estimation of lethal equivalents in the Tajima beef cattle

  Takeshi Honda, Kenji Oyama

  Nov. 2019, 日本畜産学会報, 90(4) (4), 337 - 340, Japanese

  [Refereed]

  Scientific journal


• The SNPs in bovine MMP14 promoter influence on fat-related traits

  Hirokazu Matsumoto, Fuki Kawaguchi, Shotaro Itoh, Suzuna Yotsu, Kento Fukuda, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Elsevier BV, Jun. 2019, Meta Gene, 20, 100558 - 100558, English

  [Refereed]

  Scientific journal


• Image Extraction Based on Depth Information for Calf Body Weight Estimation

  Naoki Fukuda, Takenao Ohkawa, Chikara Ohta, Kenji Oyama, Yumi Takaki, Ryo Nishide

  Jun. 2019, 12th European Federation for Information Technology in Agriculture, Food and the Environment (EFITA) International Conference, English

  [Refereed]

  International conference proceedings


• Detection of Approaching Interaction with Cattle in Estrus Based on Community Transition and Cattle Distance

  Shunta Fukumoto, Ryo Nishide, Yumi Takaki, Chikara Ohta, Kenji Oyama, Takenao Ohkawa

  Jun. 2019, 12th European Federation for Information Technology in Agriculture, Food and the Environment (EFITA) International Conference, English

  [Refereed]

  International conference proceedings


• Whole‐genome resequencing to identify candidate genes for the QTL for oleic acid percentage in Japanese Black cattle

  Fuki Kawaguchi, Hiroto Kigoshi, Moriyuki Fukushima, Eiji Iwamoto, Eiji Kobayashi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Wiley, Apr. 2019, Animal Science Journal, 90(4) (4), 467 - 472, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討

  山本 雷斗, 川口 芙岐, 秋山 敬孝, 吉田 恵実, 小浜 菜美子, 小林 栄治, 大山 憲二, 万年 英之, 笹崎 晋史

  (公社)日本畜産学会, Mar. 2019, 日本畜産学会大会講演要旨集, 125回, 177 - 177, Japanese


• A study on outdoor localization method by recurrent deep learning based on time series of received signal strength from low power wireless tag

  Takahiro Yamanishi, Takuto Jikyo, Tomio Kamada, Ryo Nishide, Chikara Ohta, Kenji Oyama, Takenao Ohkawa

  Institute of Electronics, Information and Communications Engineers ({IEICE}), 2019, IEICE Communications Express

  [Refereed]


• A study on outdoor localization method based on deep learning using model-based received power estimation data of low power wireless tag

  Takuto Jikyo, Takahiro Yamanishi, Tomio Kamada, Ryo Nishide, Chikara Ohta, Kenji Oyama, Takenao Ohkawa

  Institute of Electronics, Information and Communications Engineers ({IEICE}), 2019, IEICE Communications Express

  [Refereed]


• Effect of STARD3 gene polymorphism on carcass traits and fatty acid composition in Japanese Black cattle

  Hiroto Kigoshi, Fuki Kawaguchi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Japanese Society of Animal Breeding and Genetics, 2019, The Journal of Animal Genetics, 47(2) (2), 37 - 45

  [Refereed]

  Scientific journal


• Pool-based genome-wide association study identified novel candidate regions on BTA9 and 14 for oleic acid percentage in Japanese Black cattle

  Fuki Kawaguchi, Hiroto Kigoshi, Ayaka Nakajima, Yuta Matsumoto, Yoshinobu Uemoto, Moriyuki Fukushima, Emi Yoshida, Eiji Iwamoto, Takayuki Akiyama, Namiko Kohama, Eiji Kobayashi, Takeshi Honda, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Wiley, Aug. 2018, Animal Science Journal, 89(8) (8), 1060 - 1066, English

  [Refereed]

  Scientific journal


• A genome-wide association study for fat-related traits computed by image analysis in Japanese Black cattle

  Ayaka Nakajima, Fuki Kawaguchi, Yoshinobu Uemoto, Moriyuki Fukushima, Emi Yoshida, Eiji Iwamoto, Takayuki Akiyama, Namiko Kohama, Eiji Kobayashi, Takeshi Honda, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Wiley, May 2018, Animal Science Journal, 89(5) (5), 743 - 751, English

  [Refereed]

  Scientific journal


• ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索

  川口 芙岐, 木越 洸利, 小浜 菜美子, 秋山 敬孝, 福島 護之, 吉田 恵実, 小林 栄治, 大山 憲二, 万年 英之, 笹崎 晋史

  (公社)日本畜産学会, Mar. 2018, 日本畜産学会大会講演要旨集, 124回, 111 - 111, Japanese


• Calf Weight Estimation with Stereo Camera Using Three-Dimensional Successive Cylindrical Model

  Ayumi Yamashita, Takenao Ohkawa, Kenji Oyama, Chikara Ohta, Ryo Nishide, Takeshi Honda

  Various studies have been conducted on methods for estimating the weight of cattle. In this study, we propose a method to estimate body weight by modeling the shape of calf using three-dimensional information extracted from the stereo images. Initially, a stereo camera is set with two fixed network cameras, to take a motion image of a calf. Three-dimensional coordinate is calcu

  The Institute of Industrial Applications Engineers, Jan. 2018, Journal of the Institute of Industrial Applications Engineers (JIIAE), 6(1) (1), 39 - 46, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Research Article Effect of the PLAG1 gene polymorphism on oleic acid percentage in Japanese Black cattle populations

  H. Kigoshi, F. Kawaguchi, R. Yasuzumi, K. Oyama, H. Mannen, S. Sasazaki

  Genetics and Molecular Research, 2018, Genetics and Molecular Research, 17(3) (3), 18056, English

  [Refereed]

  Scientific journal


• Refined Cattle Detection Using Composite Background Subtraction and Brightness Intensity from Bird's Eye Images.

  Mami Aotani, Ryo Nishide, Yumi Takaki, Chikara Ohta, Kenji Oyama, Takenao Ohkawa

  ACM, Dec. 2018, Proceedings of the Ninth International Symposium on Information and Communication Technology(SoICT), 243 - 250, English

  [Refereed]

  International conference proceedings


• Quantifying the Approaching Behaviors for Interactions in Detecting Estrus of Breeding Cattle.

  Shunta Fukumoto, Ryo Nishide, Yumi Takaki, Chikara Ohta, Kenji Oyama, Takenao Ohkawa

  ACM, Dec. 2018, Proceedings of the Ninth International Symposium on Information and Communication Technology(SoICT), 235 - 242, English

  [Refereed]

  International conference proceedings


• Cattle Community Extraction Using the Interactions Based on Synchronous Behavior.

  Yohei Yamauchi, Ryo Nishide, Yumi Takaki, Chikara Ohta, Kenji Oyama, Takenao Ohkawa

  ACM, Dec. 2018, Proceedings of the Ninth International Symposium on Information and Communication Technology(SoICT), 227 - 234, English

  [Refereed]

  International conference proceedings


• Calf Robust Weight Estimation Using 3D Contiguous Cylindrical Model and Directional Orientation from Stereo Images.

  Ryo Nishide, Ayumi Yamashita, Yumi Takaki, Chikara Ohta, Kenji Oyama, Takenao Ohkawa

  ACM, Dec. 2018, Proceedings of the Ninth International Symposium on Information and Communication Technology(SoICT), 208 - 215, English

  [Refereed]

  International conference proceedings


• 鹿児島県産黒毛和種における損徴発生状況に関する調査研究

  小山 秀美, 今村 清人, 坂元 信一, 西 和隆, 井上 慶一, 河邊 弘太郎, 岡本 新, HONDA TAKESHI, OYAMA KENJI, 下桐 猛

  Defects in appearance of Japanese Black cattle are not only impairing the characteristics of the breed but also inflicting economic losses on farmers in Japan. The objective of this study was to investigate the incidence of the four kinds of defects using 511,337 calf test records of Japanese Black heifer calves born in Kagoshima prefecture in April 1999 through March 2015. The defects included were white spotting (WS), tongue's defect (TD), nipple's defect (ND) and defect of coat color (DC). The defect incidence rate ranged from 0.29% for DC to 3.68% for WS. All incidences increased from 1999 to 2015. In addition, site of occurrence for WS has changed, and a decreased trend in ratio of udder-specific WS was observed. Regression coefficients of the incidences of WS on the level of inbreeding were significantly positive (P<0.05). The defect incidence of daughters was significantly different between normal and defective dams (P<0.01) except for DC. Moreover, highly positive correlations were found between the sire-by-sire incidence rates crossed with normal dams and defective dams in WS and TD, suggesting that sires could be influential factor for the incidence. Our results showed the possibility to reduce the appearance defects of Japanese Black by considering the mates.

  Japanese Society of Animal Science, Nov. 2017, 日本畜産学会報, 88(4) (4), 425 - 430, Japanese

  [Refereed]

  Scientific journal


• Preservation of Genetic Diversity of the Asian Native Goats

  Honda T, Shibano M, Matsumoto H, Sasazaki S, Oyama K, Mannen H

  Sep. 2017, Animal Veterinary Sciences, 5, 69 - 72, English

  [Refereed]

  Scientific journal


• Estimation of Calf Weight from Fixed-Point Stereo Camera Images Using Three-Dimensional Successive Cylindrical Model

  Ayumi Yamashita, Takenao Ohkawa, Kenji Oyama, Chikara Ohta, Ryo Nishide, Takeshi Honda

  Various studies have been conducted on methods for estimating the weight of cattle. In this study, we propose a method to estimate body weight by modeling the shape of calf using three-dimensional information extracted from the stereo images. Initially, a stereo camera is set with two fixed network cameras, to take a motion image of a calf. Three-dimensional coordinate is calcu

  The Institute of Industrial Applications Engineers, Jul. 2017, The 5th IIAE International Conference on Intelligent Systems and Image Processing 2017 (ICISIP2017), 247 - 254, English

  [Refereed]

  International conference proceedings


• Detecting and Tracking Breeding Cows from Bird’s Eye Video of Pasture

  Ryo Nishide, Yoji Hosomi, Takenao Ohkawa, Kenji Oyama, Chikara Ohta

  Breeding cows are known to engage in social activities, such as interacting with other cows and forming groups similarly to humans. This paper proposes a method to extract information of interaction between cows, regarding it as a primary element of sociality. In order to extract such information, we record bird's eye pictures in a pasture by a fixed point camera set on a silo

  The Institute of Industrial Applications Engineers, Jul. 2017, The 5th IIAE International Conference on Intelligent Systems and Image Processing 2017 (ICISIP2017), 239 - 246, English

  [Refereed]

  International conference proceedings


• Identification of leptin gene polymorphisms associated with carcass traits and fatty acid composition in Japanese Black cattle

  Fuki Kawaguchi, Kazuki Okura, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Previous studies have indicated that some leptin gene polymorphisms were associated with economically important traits in cattle breeds. However, polymorphisms in the leptin gene have not been reported thus far in Japanese Black cattle. Here, we aimed to identify the leptin gene polymorphisms which are associated with carcass traits and fatty acid composition in Japanese Black cattle. We sequenced the full-length coding sequence of leptin gene for eight Japanese Black cattle. Sequence comparison revealed eight single nucleotide polymorphisms (SNPs). Three of these were predicted to cause amino acid substitutions: Y7F, R25C and A80V. Then, we genotyped these SNPs in two populations (JB1 with 560 animals and JB2 with 450 animals) and investigated the effects on the traits. Y7F in JB1 and A80V in JB2 were excluded from statistical analysis because the minor allele frequencies were low (<0.1). Association analysis revealed that Y7F had a significant effect on the dressed carcass weight in JB2; R25C had a significant effect on C18:0 and C14:1 in JB1 and JB2, respectively; and A80V had a significant effect on C16:0, C16:1, C18:1, monounsaturated fatty acid and saturated fatty acid in JB1. The results suggested that these SNPs could be used as an effective marker for the improvement of Japanese Black cattle.

  WILEY, Mar. 2017, ANIMAL SCIENCE JOURNAL, 88(3) (3), 433 - 438, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Genetic relationships between meat quality traits and fatty acid composition in Japanese black cattle

  Keiichi Inoue, Noriaki Shoji, Takeshi Honda, Kenji Oyama

  Genetic parameters were estimated to investigate the relationships between meat quality traits and fatty acid composition from 11855 Japanese Black cattle. The meat quality traits included beef marbling score (BMS), beef color score, firmness of beef (FIR), texture of beef (TEX) and beef fat color score (BFS). The data on fatty acid composition included oleic acid and monounsaturated fatty acids (MUFA) contents, the ratio of MUFA to saturated fatty acids (MUS) and the ratio of elongation. The heritability estimates for meat quality traits ranged from moderate (0.30) to high (0.72). The strong genetic correlations between them were useful for simultaneous genetic improvement. In addition, high heritability estimates of fatty acid composition, ranging from 0.60 to 0.63, indicated that they could also be improved genetically. The genetic correlations of fatty acid composition with BMS, FIR and TEX were weak and negative. In contrast, positive and stronger genetic correlations were found between BFS and fatty acid composition, in particular, related to the level of unsaturation (0.77 and 0.79 for MUFA and MUS, respectively). This implies that improving the level of unsaturation makes fat darker (more yellow) and thus requires balancing with BFS.

  WILEY-BLACKWELL, Jan. 2017, ANIMAL SCIENCE JOURNAL, 88(1) (1), 11 - 18, English

  [Refereed]

  Scientific journal


• The effect of LPL gene polymorphisms on fatty acid composition in Japanese Black cattle

  Fuki KAWAGUCHI, Satoshi KOGA, Kenji OYAMA, Hideyuki MANNEN,, Shinji SASAZAKI

  Japanese Society of Animal Breeding and Genetics, 2017, The Journal of Animal Genetics, 45(1) (1), 3 - 8, Japanese

  [Refereed]

  Scientific journal


• Inferring phenotypic causal structures among meat quality traits and the application of a structural equation model in Japanese Black cattle

  K. Inoue, B. D. Valente, N. Shoji, T. Honda, K. Oyama, G. J. M. Rosa

  Meat quality is one of the most important traits determining carcass price in the Japanese beef market. Optimized breeding goals and management practices for the improvement of meat quality traits requires knowledge regarding any potential functional relationships between them. In this context, the objective of this research was to infer phenotypic causal networks involving beef marbling score (BMS), beef color score (BCL), firmness of beef (FIR), texture of beef (TEX), beef fat color score (BFS), and the ratio of MUFA to SFA (MUS) from 11,855 Japanese Black cattle. The inductive causation (IC) algorithm was implemented to search for causal links among these traits and was conditionally applied to their joint distribution on genetic effects. This information was obtained from the posterior distribution of the residual (co)variance matrix of a standard Bayesian multiple trait model (MTM). Apart from BFS, the IC algorithm implemented with 95% highest posterior density (HPD) intervals detected only undirected links among the traits. However, as a result of the application of 80% HPD intervals, more links were recovered and the undirected links were changed into directed ones, except between FIR and TEX. Therefore, 2 competing causal networks resulting from the IC algorithm, with either the arrow FIR -> TEX or the arrow FIR -> TEX, were fitted using a structural equation model () to infer causal structure coefficients between the selected traits. Results indicated similar genetic and residual variances as well as genetic correlation estimates from both structural equation models. The genetic variances in BMS, FIR, and TEX from the structural equation models were smaller than those obtained from the MTM. In contrast, the variances in BCL, BFS, and MUS, which were not conditioned on any of the other traits in the causal structures, had no significant differences between the structural equation model and MTM. The structural coefficient for the path from MUS (BCL) to BMS showed that a 1-unit improvement in MUS (BCL) resulted in an increase of 0.85 or 1.45 (an decrease of 0.52 or 0.54) in BMS in the causal structures. The analysis revealed some interesting functional relationships, direct genetic effects, and the magnitude of the causal effects between these traits, for example, indicating that BMS would be affected by interventions on MUS and BCL. In addition, if interventions existed in this scenario, a breeding strategy based only on the MTM would lead to a mistaken selection for BMS.

  AMER SOC ANIMAL SCIENCE, Oct. 2016, JOURNAL OF ANIMAL SCIENCE, 94(10) (10), 4133 - 4142, English

  [Refereed]

  Scientific journal


• Estimation of genetic parameters for carcass defects of Japanese Black cattle in Kagoshima

  Kazutaka Nishi, Takeshi Shimogiri, Akinori Kusano, Shinichi Sakamoto, Kiyomi Shiromoto, Kotaro Kawabe, Shin Okamoto, Takeshi Honda, Kenji Oyama

  Cattle exhibit a range of carcass defects, including blood splash (BLS), intramuscular edema (INE), muscle steatosis (MUS), bruising (BR), trim loss (TRL) and others (OTH). These defects lower the carcass value and can result in significant economic loss to producers. We estimated the incidence, relationship with inbreeding coefficients and genetic parameters of carcass defects in Japanese Black cattle using 561 619 carcass records from Kagoshima, Japan during April 1988 through March 2011. The defect incidence ranged from 0.22% for TRL to 5.73% for BR. The incidence of MUS and BR increased from 1.21% to 6.57% and from 1.06% to 9.31%, respectively. The incidence of INE peaked at 7.44% in 1999 and decreased thereafter. We observed a positive linear relationship between the defect incidence and the inbreeding coefficients in MUS, BR and TRL (P<0.01). The heritabilities estimated by univariate animal model with Gibbs sampling for BLS, INE, MUS, BR and TRL were 0.24, 0.06, 0.18, 0.05 and 0.02, respectively. The contribution of farm variance to phenotypic variance was negligible (0.01 to 0.04). Significant genetic correlations of TRL were estimated with MUS (0.63) and BR (0.63). Our results suggest that genetic factors contribute to the incidence of BLS and MUS.

  WILEY-BLACKWELL, May 2016, ANIMAL SCIENCE JOURNAL, 87(5) (5), 655 - 660, English

  [Refereed]

  Scientific journal


• Economic losses related to internal diseases in Japanese black cattle

  Keiichi Inoue, Takeshi Honda, Kenji Oyama

  The objective of this study was to estimate the direct economic losses due to the condemnation of the liver and large intestine because of internal diseases (multifocal necrosis in the liver (MNL) and inflammation of the large intestine (ILI)), and the indirect losses because of reductions in carcass performance from MNL, bovine abdominal fat necrosis (BFN) and ILI using data from 5383 Japanese Black cattle. Direct losses were estimated by multiplying the price of the condemned part by the frequency of its occurrence owing to the disease. Similarly, indirect losses were estimated as the product of unit carcass price and reduction in carcass weight (CW) due to the disease. The direct impact on the beef cattle industry from MNL and ILI was estimated at around $1.29 million (US$1 = (sic)120) per year. A least-squares analysis showed that MNL had no influence on any carcass trait, whereas BFN and ILI significantly reduced CW, rib eye area and darkened the beef. ILI also reduced rib thickness. The indirect losses from BFN and ILI were estimated as a maximum of $131.7 and $256.4 per animal and around $6.26 million and $4.03 million for the industry, respectively, mostly because of the reduction in CW.

  WILEY-BLACKWELL, May 2016, ANIMAL SCIENCE JOURNAL, 87(5) (5), 736 - 741, English

  [Refereed]

  Scientific journal


• Change of fatty acid composition of the lumbar longissimus during the final stage of fattening in the Japanese Black cattle

  Takeshi Honda, Takafumi Ishida, Ikuo Kobayashi, Yusuke Oguri, Yuto Mizuno, Hideyuki Mannen, Hiroaki Iwaisaki, Shiro Kuge, Kunihiko Saito, Kenji Oyama

  Consideration of the shortened fattening period seems to be worthwhile for the realization of profitable beef production. In this study, change of fatty acid composition of the lumbar longissimus during the final stage of fattening was investigated in Japanese Black cattle. Each of 110 fattening animals was sampled three times: the initial two samples were taken by biopsy (25.7 months and 27.5 months on average) and the final one was from carcasses (29.9 months on average). Preliminary analysis indicated that removing muscle tissues from the constant body position of the living animals should be essential for sampling. Average monounsaturated fatty acids (MUFA) at three sampling points were 58.1%, 58.5% and 60.5%, and the differences of the third sampling with the first and second samplings were significant. Both in steers and heifers, MUFA also increased as the fattening stage proceeded, and MUFA of the heifers at all the sampling points were significantly higher than those of the steers. The increasing rate of MUFA rose from 0.21 percentage points (pp)/month at period 1 (from the first sampling to the second sampling) to 0.84 pp/month at period 2 (from the second sampling to the slaughter).

  WILEY-BLACKWELL, Apr. 2016, ANIMAL SCIENCE JOURNAL, 87(4) (4), 578 - 583, English

  [Refereed]

  Scientific journal


• Extraction of interaction information between breeding cows with GPS and its application to estrus detection.

  Kengo Matsumoto, Kenji Oyama, Takeshi Honda, Takenao Ohkawa

  This study focuses on bovine sociality and proposes a new method of detecting estrus through the detection and the analysis of interactions between cows. In the proposed method, we mainly trace bovine behavior when an animal approaches other cows based on their time-series location data measured by a GPS installed on each animal. The cow's estrus is detected by analyzing the interaction information extracted from the behavior. Our proposed method experimentally obtained more accurate results than the existing method of estrus detection.

  IEEE, 2016, 2016 International Joint Conference on Neural Networks(IJCNN), 2967 - 2972, English

  [Refereed]

  International conference proceedings


• A method of detecting interaction between breeding cows from time-series bird's eye pictures of pasture.

  Yoji Hosomi, Kenji Oyama, Takeshi Honda, Takenao Ohkawa

  Breeding cows are known to engage in sociality, in which they interact and form groups. This paper proposes a method of detecting the interaction between breeding cows from time-series pictures of pastures by a similar image retrieval method using a Bag of Visual Words. We divided the interaction detection into three tasks: detecting a pair of cows in an interaction, pinpointing the time and the place that the interaction was observed and identifying its type. This paper focuses on the identifying task. In the proposed method, a similar image retrieval method is used to classify the interaction, and the information from the states of preceding and succeeding the interaction is considered to improve the accuracy of the interaction classification. The effectiveness of the proposed method was confirmed experimentally.

  IEEE, 2016, 2016 International Joint Conference on Neural Networks(IJCNN), 2973 - 2978, English

  [Refereed]

  International conference proceedings


• The effect of leptin gene polymorphisms on fertility traits in Japanese Black cattle

  Fuki KAWAGUCHI, Kenji FUKAZAWA, Namiko KOHAMA, Takayuki AKIYAMA, Moriyuki FUKUSHIMA, Eiji KOBAYASHI, Kenji OYAMA, Hideyuki MANNEN, Shinji SASAZAKI

  Japanese Society of Animal Science, 2016, Nihon Chikusan Gakkaiho, 87(4) (4), 333 - 338, Japanese

  [Refereed]

  Scientific journal


• The g.841G > C SNP of FASN gene is associated with fatty acid composition in beef cattle

  Kiri Hayakawa, Takayuki Sakamoto, Atsushi Ishii, Keita Yamaji, Yoshinobu Uemoto, Nanae Sasago, Eiji Kobayashi, Naohiko Kobayashi, Tamako Matsuhashi, Shin Maruyama, Hirokazu Matsumoto, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  The objective of the current study is to evaluate the association between fatty acid composition and fatty acid synthase gene polymorphisms as responsible mutations. For this purpose, we selected seven previously reported single nucleotide polymorphisms (SNPs) in FASN gene, including one within promoter region (g.841G>C) and six non-synonymous SNPs (g.8805C>T, g.13126C>T, g.15532A>C, g.16024A>G, g.16039C>T, g.17924A>G), and genotyped them in Japanese Black cattle. Genotyping results revealed that g.8805 C>T and g.17924 A>G were monomorphic loci. Genome-wide association analysis including the other five SNPs revealed that only g.841G>C showed significant associations with the percentages of C14:0, C14:1, C16:1 and C18:1 at 5% genome-wide significance level. In order to further evaluate the effect, we genotyped g.841G>C using additional three populations, including two Japanese Black populations and a Holstein cattle population. g.16024A>G was also genotyped and included in the analysis because it has been reported to be associated with fatty acid composition in Japanese Black cattle. In the result of analysis of variance, g.841G>C showed stronger effects on fatty acid percentage than those of g.16024A>G in all populations. These results suggested that g.841G>C would be a responsible mutation for fatty acid composition and contribute to production of high-grade beef as a selection marker in beef cattle.

  WILEY-BLACKWELL, Aug. 2015, ANIMAL SCIENCE JOURNAL, 86(8) (8), 737 - 746, English

  [Refereed]

  Scientific journal


• 放牧場の俯瞰動画データを用いた繁殖牛のインタラクション検出

  HOSOMI YOJI, OHKAWA TAKENAO, OYAMA Kenji, HONDA Takeshi

  May 2015, 電気学会 第62回情報システム研究会, IS-15-009, Japanese

  Symposium


• GPSを利用した放牧牛のインタラクション情報の取得とその分析

  MATSUMOTO Kengo, OHKAWA TAKENAO, OYAMA Kenji, HONDA Takeshi

  May 2015, 電気学会 第62回情報システム研究会, IS-15-001, Japanese

  Symposium


• Genetic relationships between internal diseases diagnosed at slaughter and carcass traits in Japanese Black cattle

  Inoue K, Honda T, Oyama K

  May 2015, Journal of Animal Science, 93(6) (6), 2714 - 2721, English

  [Refereed]

  Scientific journal


• Searching for phenotypic causal links among meat quality traits in Japanese Black cattle

  Inoue K, Valente DB, Shoji N, HONDA TAKESHI, OYAMA KENJI, Rosa JMG

  Aug. 2014, Proceedings of 10th World Congress on Genetics Applied to Livestock Production, 749, English

  [Refereed]

  International conference proceedings


• UTS2R gene polymorphisms are associated with fatty acid composition in Japanese beef cattle

  Shinji Sasazaki, Kento Akiyama, Takahiro Narukami, Hirokazu Matsumoto, Kenji Oyama, Hideyuki Mannen

  Fatty acid composition of beef adipose tissue is one of its important traits because a high proportion of monounsaturated fatty acid is related to favorable beef flavor and tenderness. In this study, we searched polymorphisms in full length coding DNA sequence of urotensin 2 recepter and investigated the effects on fatty acid composition (C14:0, C14:1, C16:0, C16:1, C18:0, C18:1, C18:2, monounsaturated fatty acid, saturated fatty acid). Eight single nucleotide polymorphisms (SNP) were identified by sequence comparison among eight animals, including five Japanese Black and three Holstein cattle. One of these SNP (c.866C>T) was predicted to cause amino acid substitutions (P289L) and the other seven synonymous SNP, including c.267C>T, were presumed to be in linkage disequilibrium. Therefore we selected two SNP (c.267C>T and c.866C>T) for further analysis. We investigated associations between these genotypes and fatty acid composition in three Japanese Black populations (n=560, 245 and 287) and a Holstein population (n=202). Tukey-Kramer's honestly significant difference test revealed that CC genotype in c.267C>T indicated lower C14:0 and higher C18:1 than the other genotypes in Japanese Black cattle and CC genotype in c.866C>T showed lower C16:1 than CT genotype in Holstein cattle (P<0.05). These results suggested that these genotypes would contribute to production of high-grade meat as selection markers in beef cattle.

  WILEY-BLACKWELL, May 2014, ANIMAL SCIENCE JOURNAL, 85(5) (5), 499 - 505, English

  [Refereed]

  Scientific journal


• The SNPs in the promoter regions of the bovine FADS2 and FABP4 genes are associated with beef quality traits

  Hirokazu Matsumoto, Takuya Nogi, Ichiro Tabuchi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Genetic factors are known to influence meat quality in beef cattle. Expression of fatty acid desaturase 2 (FADS2) and fatty acid-binding protein 4 (FABP4) genes in the fat metabolism pathway has been reported to influence fat-related traits. In this study, we aimed to discover DNA polymorphisms in the promoter regions of the FADS2 and FABP4 genes in cattle populations to develop genetic markers. As a consequence, one single nucleotide polymorphism (SNP) in the FADS2 promoter (FADS2 g.-823G > A) and two linked SNPs in the FABP4 promoter (FABP4 g.-295A > G and FABP4 g.-287A > G) were identified. All of them were assumed to influence binding sites for transcription factors, suggesting these SNPs influence the genes' expression and beef quality. Therefore, we performed association analysis between the genotypes and traits. Our results revealed that the FADS2 g.-823G > A had significant effects on several traits including beef marbling score (BMS). BMS of the G/G animals in a Holstein population (2.36) was higher than that of the G/A animals (2.13). The same tendency was observed in Japanese Black populations. On the other hand, although the FABP4 SNPs also had significant effects on some traits, they did not show additive effects on any of the traits analyzed in this study. Our results suggest that the FADS2 g.-823G > A is a useful genetic marker for breeding in beef cattle. (C) 2014 Elsevier B.V. All rights reserved.

  ELSEVIER SCIENCE BV, May 2014, LIVESTOCK SCIENCE, 163, 34 - 40, English

  [Refereed]

  Scientific journal


• Effects of genes on economically important traits of Japanese Black cattle in Hyogo population

  Ohkura K, Akiyama T, YOSHIDA E, FUKUSHIMA M, IWAMOTO E, OKA A, MATSUMOTO H, SASAZAKI Shinji, OYAMA Kenji, MANNEN Hideyuki

  In this study, we investigated allelic frequencies and genetic effects of SCD, SREBP-1, FASN, EDG-1, F11, MC1R and CW-2 genes on beef carcass traits including fatty acid composition in the closed Hyogo population of Japanese Black cattle. The favorable allele frequencies were 0.960 in SCD, 0.322 in SREBP-1, 0.910 in FASN, 0.265 in EDG-1, 0.623 in F11, 0.464 in MC1R and 0.007 in

  Nihon Chikusan Gakkai, Nov. 2013, Nihon Chikusan Gakkaiho, 84(2) (2), 157 - 162, Japanese

  [Refereed]

  Scientific journal


• 兵庫系黒毛和種における育種集団毎の遺伝的多様性評価

  福島 護之, 本多 健, 小路 怜子, 小浜 菜美子, 秋山 敬孝, 坂瀬 充洋, 岡 章生, 大山 憲二

  (公社)日本畜産学会, Sep. 2013, 日本畜産学会大会講演要旨集, 117回, 65 - 65, Japanese


• Identification of quantitative trait loci affecting economic traits based on divergently selected genomic regions between beef and dairy cattle

  Keita Yamaji, Daisuke Hosokawa, Atsushi Ishii, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  In our previous study, we examined divergently selected regions between Japanese Black cattle and Holstein cattle based on a 50k single nucleotide polymorphism (SNP) panel and a Sliding Window Allele Difference method resulting in the identification of 11 genomic regions. The aim of the present study was to investigate the association between these genomic regions and economic traits, including seven carcass and five milk production traits. For this purpose, representative SNP markers were selected from the 11 genomic regions and used to estimate the effects on the traits in Japanese Black cattle (N=488) and Holstein cattle (N=194). Association analyses revealed that five SNPs showed a significant effect on the carcass traits in Japanese Black cattle and other five SNP showed a significant association with milk production traits in Holstein cattle (P< 0.05). These results indicated that divergently selected regions identified using a Sliding Window Allele Difference method contain the quantitative trait loci (QTL) for economic traits. Furthermore, most of the QTL identified in this study were consistent with the previously reported QTL. Further investigations of these regions may lead to the identification of the genes and polymorphisms responsible for the economic traits. © 2013 Elsevier B.V.

  Elsevier, Aug. 2013, Livestock Science, 155(2-3) (2-3), 180 - 185, English

  [Refereed]

  Scientific journal


• Comparative analysis on gene expression profiles in longissimus dorsi muscle of Japanese Black cattle

  MATSUMOTO H, OGURI Y, MIZUNO Y, ISHIDA T, KOBAYASHI I, IWAISAKI H, SAITO K, SASAGO N, KOBAYASHI E, KUGE S, HONDA Takeshi, SASAZAKI Shinji, OYAMA Kenji, MANNEN Hideyuki

  Recently, fatty acid composition has been recognized as an important economic trait of Japanese Black cattle. The aim of this study was to seek for candidate genes which might be useful for beef breeding to increase the mono-unsaturated fatty acid (MUFA) level. For this purpose, we compared gene expression profile of Japanese Black cattle which showed high percentage of MUFA in

  日本動物遺伝育種学会, Jun. 2013, The Journal of Animal Genetics, 41(1) (1), 7 - 14, Japanese

  [Refereed]

  Scientific journal


• The SNP in the promoter region of the bovine ELOVL5 gene influences economic traits including subcutaneous fat thickness

  Hirokazu Matsumoto, Yumiko Shimizu, Atsuko Tanaka, Takuya Nogi, Ichiro Tabuchi, Kenji Oyama, Masaaki Taniguchi, Hideyuki Mannen, Shinji Sasazaki

  Genetic analyses have contributed to improvements of economically important traits derived from adipose tissue such as fatty acid composition in beef. Elongation of very long chain fatty acids (ELOVL) genes encode for the enzymes that play an important role in elongation of long-chain fatty acids. In this study, we aimed to discover genetic polymorphisms of ELOVL gene family in cattle populations to develop genetic markers. As a result, five synonymous mutations were detected in the coding regions of the ELOVL1, ELOVL2, ELOVL3 and ELOVL5 genes. In addition, six mutations were identified in promoter region of the ELOVL5. Two of five mutations in the promoter region of ELOVL5 were expected to alter the ELOVL5 expression and influence the economic traits, because of the high synteny of the region which was essential for activation of Elovl5 in mouse. Therefore, we performed association analysis between the genotypes and traits and our result revealed that T allele of g.-110T > C in ELOVL5 gene promoter indicated significantly thinner subcutaneous fat thickness (TT, 2.39 cm; CT, 2.35 cm) than that of C allele (CC, 2.68 cm) in a Japanese Black population. Our results suggest that the g.-110T > C is a useful genetic marker for the breeding in beef cattle.

  SPRINGER, Apr. 2013, MOLECULAR BIOLOGY REPORTS, 40(4) (4), 3231 - 3237, English

  [Refereed]

  Scientific journal


• The SNPs in the ACACA gene are effective on fatty acid composition in holstein milk

  Hirokazu Matsumoto, Kenta Sasaki, Takuya Bessho, Eiji Kobayashi, Tsuyoshi Abe, Shinji Sasazaki, Kenji Oyama, Hideyuki Mannen

  Fatty acid composition is an important economic trait for both dairy and beef cattle and controlled by genetic factors. Candidate genes controlling fatty acid composition may be found in fat synthesis and metabolism pathways. Acetyl-CoA carboxylase is the flux-determining enzyme in the regulation of fatty acid synthesis in animal tissues. One of two isozymes of this enzyme, acetyl-CoA carboxylase-alpha (ACACA), catalyses the first committed step of fatty acid synthesis in mammalian cytosol, leading to the biosynthesis of long-chain fatty acids. In the current study, the sequence comparison of the coding sequence (CDS) and two promoter regions (PIA and PIII) in bovine ACACA gene was performed between Japanese Black and Holstein cattle to detect nucleotide polymorphisms influencing fatty acid composition in milk and beef. Five single nucleotide polymorphisms (SNPs) were identified in the CDS region, 28 SNPs in the PIA region and three SNPs in the PIII region. Association study revealed that CCT/CCT type of PIII_#1, #2/PIA_#26 indicated a higher percentage of C14:0 in the milk of the Holstein cattle than CCT/GTC type (p = 0.050) and that a difference of the percentage of C16:0 was observed between CCT/CCT and GTC/GTC type (p = 0.023). CDS_#2 T/T type indicated a higher percentage of C18:0 than T/C type (p = 0.008). In addition, the Japanese Black cattle with CC/GT type of PIII_#1, #2 showed a higher percentage of C18:2 in the meat than those with GT/GT type (p = 0.025). Since PIII is the promoter specific to mammary gland during lactation, the altered expression of the ACACA gene owing to the SNPs in the PIII region may influence the fatty acid composition in the milk.

  SPRINGER, Sep. 2012, MOLECULAR BIOLOGY REPORTS, 39(9) (9), 8637 - 8644, English

  [Refereed]

  Scientific journal


• Sampling method for estimating neutral allele frequency in a pedigreed population

  Takeshi Honda, Shinji Sasazaki, Kenji Oyama, Fumio Mukai, Tetsuro Nomura

  The estimated allele frequency at neutral DNA marker loci is a fundamental parameter for establishing a conservation scheme for a set of livestock breeds. In this study, we propose a novel minimum distance (MD) method for estimating neutral allele frequencies, which minimizes the error by the use of pedigree information. Using computer simulation and actual microsatellite data for a pedigreed cattle population, the performance of the proposed method was compared with that of conventional random sampling (RND). MD manifests a promising superiority to RND, irrespective of the sample size and the depth of pedigree. It is theoretically demonstrated that the error of the estimates depends on both the sample size and the distance of founder allele frequencies between the sampled and the non-sampled individuals. MD samples individuals so as to minimize the latter source of estimation error.

  WILEY-BLACKWELL, Jun. 2012, JOURNAL OF ANIMAL BREEDING AND GENETICS, 129(3) (3), 226 - 233, English

  [Refereed]

  Scientific journal


• Identification of SNPs in the FASN gene and their effect on fatty acid milk composition in Holstein cattle

  Hirokazu Matsumoto, Satoshi Inada, Eiji Kobayashi, Tsuyoshi Abe, Hiroyuki Hasebe, Shinji Sasazaki, Kenji Oyama, Hideyuki Mannen

  FASN is a multifunctional enzyme that catalyzes de novo biosynthesis of long-chain saturated fatty acid, and alteration of this enzyme may affect economic traits including fatty acid composition. Sequencing the coding sequence (CDS) of the FASN gene has so far been done only partially for dairy cattle. In this study, the CDS of the FASN gene of eight Holstein cattle was sequenced to detect nucleotide polymorphisms. We could identify 13 single nucleotide polymorphisms (SNPs) of which five were non-synonymous mutations (H1390Y, I1856L, T1950A, W1955R and T2264A). The association between these SNPs and economic traits was analyzed using 198 Holstein dams. Genotypes of H1390Y corresponded to those of the I1856L in the group, suggesting these SNPs were linked. Furthermore, the T1950A was suggestively linked with the W1955R. The association study revealed that T1950A/W1955R had an effect on milk fat content and the C14 Index. This will help to understand the complex association between the FASN gene and economic traits. (C) 2011 Elsevier B.V. All rights reserved.

  ELSEVIER SCIENCE BV, Apr. 2012, LIVESTOCK SCIENCE, 144(3) (3), 281 - 284, English

  [Refereed]

  Scientific journal


• Identification of divergently selected regions between Japanese Black and Holstein cattle using bovine 50k SNP array

  Daisuke Hosokawa, Atsushi Ishii, Keita Yamaji, Shinji Sasazaki, Kenji Oyama, Hideyuki Mannen

  Differences between average allelic frequencies of genes that relate to traits suggest that it would be evidence of artificial selections. Sliding window approach is a useful method to identify genomic regions that have been differently selected between two breeds. The objective of this study was to identify the divergently selected regions between Japanese Black (JB) and Japanese Holstein (JH) cattle based on genotypic information obtained through a high-density single nucleotide polymorphism (SNP) panel. After genotyping of 54 001 SNP markers on 100 animals (50 JB and 50 JH), 40 635 SNPs were suitable for the analysis. For each of these SNPs, the absolute difference between allelic frequencies of JB and JH was calculated. In the current study, 10 consecutive SNPs were defined as components of a window. For each window, the average difference in allelic frequency was calculated. This was termed sliding window average difference (SWAD). Among 40 055 windows, we focused on 39 windows with the largest SWAD. This was equivalent to 0.1% of all windows and the SWAD was more than 0.435. Some of these windows overlapped and were distributed in 11 regions. These regions were in good agreement with reported quantitative trait locus, therefore would be selection signatures and good candidates that harbor the causative mutations.

  WILEY-BLACKWELL, 2012, ANIMAL SCIENCE JOURNAL, 83(1) (1), 7 - 13, English

  [Refereed]

  Scientific journal


• 黒毛和種におけるDGAT1遺伝子多型の枝肉形質に対する効果

  鳴神貴太, MANNEN HIDEYUKI, OYAMA KENJI, 庄司則章, 中嶋宏明, SASAZAKI SHINJI

  日本畜産学会, Nov. 2011, 日本畜産学会報, 82(2) (2), 125 - 130, Japanese

  [Refereed]

  Scientific journal


• Heritabilities and genetic correlations of fatty acid compositions in longissimus muscle lipid with carcass traits in Japanese Black cattle

  T. Nogi, T. Honda, F. Mukai, T. Okagaki, K. Oyama

  Fatty acid composition and carcass traits of 2,275 Japanese Black steers and heifers were analyzed to estimate the heritabilities and genetic correlations using the REML procedure. Slices of LM at the 6th to 7th rib section were minced and homogenized, and total lipids were extracted for the analysis by a gas chromatograph. Oleic acid accounted for the majority (51.3%), followed by palmitic (26.4%) and stearic (10.8%) acids. Heritabilities of carcass traits were moderate to high, ranging from 0.34 to 0.61, and heritabilities of individual fatty acids varied largely from 0.00 to 0.78. Those of MUFA, SFA, and PUFA were estimated to be 0.68, 0.66, and 0.47, respectively. Predicted breeding values for MUFA in 99 sires ranged from -3.0 to 5.4%. Genetic correlations of fatty acid compositions with carcass traits were generally weak (-0.28 to 0.39). Low but positive genetic correlations were obtained between beef marbling, on which emphasis of selection has been placed, and oleic acid (0.19) or MUFA (0.23). The results indicated the possibility not only for genetic improvement in fat quality traits but also simultaneous improvements with carcass traits by appropriate selection program.

  AMER SOC ANIMAL SCIENCE, Mar. 2011, JOURNAL OF ANIMAL SCIENCE, 89(3) (3), 615 - 621, English

  [Refereed]

  Scientific journal


• Estimates of milk yield until 90 days after calving in Japanese Black cows

  TSUJI Emi, HONDA Takeshi, KUGE Shiro, OYAMA Kenji

  2011, 肉用牛研究会報, 90, 10 - 14, Japanese

  [Refereed]

  Scientific journal


• Development of discrimination markers between Japanese domestic and imported beef

  Shinji Sasazaki, Daisuke Hosokawa, Rie Ishihara, Hiroki Aihara, Kenji Oyama, Hideyuki Mannen

  In the meat industry, correct labeling of beef origins or breed is required to assure quality and safety. This paper describes the development of discrimination markers between Japanese domestic and imported beef from the United States (US) and Australia (AUS) based on a bovine 50K single nucleotide polymorphism (SNP) array using a total of 110 samples: Japanese Black (n = 50), Japanese Holstein (n = 50) and US cattle (n = 10). Genotyping information revealed 1081 SNPs as candidate markers that were polymorphic only in US cattle. The genotyping results by PCR - restriction length polymorphism in Japanese Black (n = 300) and Holstein cattle (n = 146) revealed that 11 SNPs had alleles specific to US cattle. Their allelic frequencies in US cattle (n = 108) ranged from 0.097 to 0.250 with an average of 0.178 and the combined identification probability of US cattle was 0.987. In addition, we also verified the applicability of these US-specific markers to AUS cattle. Their allelic frequencies in AUS cattle (n = 280) ranged from 0.063 to 0.224 with an average of 0.137 and the combined identification probability of AUS cattle was 0.963. In conclusion, a set of these markers could be useful for discriminating between Japanese domestic and imported beef and would contribute to identify origins and prevent falsified labeling of beef.

  WILEY-BLACKWELL, 2011, ANIMAL SCIENCE JOURNAL, 82(1) (1), 67 - 72, English

  [Refereed]

  Scientific journal


• Effect of DNA polymorphisms related to fatty acid composition in adipose tissue of Holstein cattle

  Takahiro Narukami, Shinji Sasazaki, Kenji Oyama, Takuya Nogi, Masaaki Taniguchi, Hideyuki Mannen

  Fatty acid composition of adipose tissue has been recognized as an important carcass trait because of its relationship with eating quality such as favorable beef flavor and tenderness. Therefore, we investigated the effects of genetic polymorphisms of liver X receptor, alpha (LXR), stearoyl-CoA desaturase (SCD), Fatty acid synthase (FASN), and Fatty acid binding protein 4 (FABP4) on fatty acid composition in intramuscular fat tissue of Holstein steers. The major allele frequencies were 0.705 in SCD, 0.518 in FABP4, 0.888 in FASN, and 0.984 in LXR. Genotyping of SCD showed significant effect on C14:0, C14:1, C18:0 and saturated fatty acid (P < 0.05). In addition, the result suggested that SCD genotype possibly had effect on composition of C18:1 and monounsaturated fatty acid. Genotype of FABP4 had significant effect on composition of C16:0. Effect of LXR genotypes could not be analyze because of extremely biased genotype frequencies. Our results suggest that genotypes of SCD and FABP4 may in part affect meat quality in Holstein.

  WILEY-BLACKWELL, 2011, ANIMAL SCIENCE JOURNAL, 82(3) (3), 406 - 411, English

  [Refereed]

  Scientific journal


• Allele frequencies of gene polymorphisms related to economic traits in Bos taurus and Bos indicus cattle breeds

  Makoto Kaneda, Bang Zhong Lin, Shinji Sasazaki, Kenji Oyama, Hideyuki Mannen

  Allele frequencies of 10 representative polymorphisms for beef and milk traits were investigated for a total of 240 animals from Bos taurus and Bos indicus breeds, including two Japanese groups (Japanese Black and Japanese Brown), two East Asian groups (Korean and Mongolian), three European groups (Holstein, Angus and Hereford) and a Bos indicus group in South Asia (Myanmar, Laos and Cambodia). The Japanese Black revealed unique genetic construction in GH, FASN and SREBP-1 and the other Asian populations show intermediate frequencies between European and Japanese populations. The Bos indicus group showed low favorable allele frequencies in most of the genes. The study showed the variability and distribution of 10 genes affecting economic traits among world representative cattle breeds. The genetic information would contribute to elucidating the genetic background for worldwide cattle breeds and the possibility of improvement using the markers.

  WILEY-BLACKWELL, 2011, ANIMAL SCIENCE JOURNAL, 82(6) (6), 717 - 721, English

  [Refereed]

  Scientific journal


• Suppression of WWP1 Gene Via RNAi Induced the Reduction of Proliferation Rate of C2C12 Myoblasts

  Hirokazu Matsumoto, Michihiro Takahama, Ryohsuke Kajiyama, Shinji Sasazaki, Kenji Oyama, Hideyuki Mannen

  The WW domain containing E3 ubiquitin protein ligase 1 (WWP1), which plays an important role in ubiquitin-proteasome pathway to degrade unneeded or damaged proteins, was recently identified as the responsible for chicken muscular dystrophy. Despite intensive studies on oncogenic characters, the role of WWP1 to muscular diseases has not yet been fully understood. Previously, we transfected either of wild and mutated types of WWP1 gene into C2C12 mouse myoblasts to monitor the expression pattern of muscle-differentiation markers, so that excessive WWP1 expression enhanced the expression of the myosin heavy chain (MyHC) Ia gene but lowered the expression of the MyHC IIb gene, while mutated WWP1 gene transfected in to myoblasts was distinct from these cases in that the MyHC gene or genes expression inhibited the normal myoblast differentiation. However, the mechanism for the mutation to inhibit muscle differentiation remains elucidated. The current study attempted to suppress the WWP1 expression by RNAi technique and to observe its effect on C2C12 cells. The effect of WWP1 suppression was clearly different from that of the R441Q missense mutation in the WWP1 gene. The WWP1 suppression reduced the proliferation rate of C2C12 myoblasts, while clear difference was not observed in the proliferation rate by the transfection of the mutated WWP1 gene into the cells. Our RT-PCR analysis indicated that the reduction of the WWP1 expression is the specific effect mediated by RNAi and that the reduction of proliferation rate observed in this study is largely attributed to the suppression of the WWP1 expression. These data indicated that the mutation responsible for chicken muscular dystrophy does not eliminate the enzymatic activity and provides some new function for the gene.

  JAPAN POULTRY SCIENCE ASSOC, Oct. 2010, JOURNAL OF POULTRY SCIENCE, 47(4) (4), 288 - 293, English

  [Refereed]

  Scientific journal


• Mitochondrial DNA variation and genetic construction of indigenous cattle population in Bhutan

  Dorji T, Kaneda M, Lin BZ, Takahashi A, OYAMA Kenji, SASAZAKI Shinji, Yamamoto Y, Kawamoto Y, MANNEN Hideyuki

  Japanese Society of Animal Genetics, Jun. 2010, Journal of Animal Genetics, 38(1) (1), 77 - 81, English

  [Refereed]

  Scientific journal


• Genetic variability of semen characteristics in Japanese Black bulls

  NISHIMURA Kenichi, HONDA Takeshi, OYAMA Kenji

  2010, The Journal of Animal Genetics, 38(2) (2), 73 - 76, English

  [Refereed]

  Scientific journal


• Sheep genetic diversity in Bhutan using microsatellite markers

  Tashi Dorji, Han Jianlin, Po Wafula, Yoshio Yamamoto, Shinji Sasazaki, Kenji Oyama, Oliver Hanotte, Bang-Zhong Lin, Hideyuki Mannen

  Genotype data from eight microsatellite markers were used to assess genetic diversity and relationships among five indigenous Bhutanese sheep populations, Sakten, Jakar, Sarpang, Sipsu and Tsirang. Estimates of mean observed and expected heterozygosities, mean number of alleles per locus/population were obtained. The highest observed heterozygosities were found in Jakar (0.657) and Sakten (0.647), while the lowest one was found in Tsirang (0.539). Genetic distances, pairwise proportion of different alleles, UPGMA tree, and principal component analysis indicate close relationship among Tsirang, Sipsu and Sarpang populations, while Jakar and Sakten populations are located in one cluster. These two clusters are separated geographically, and show distinct phenotypic as well as molecular characters. We therefore recommend that the Bhutanese native sheep populations be classified into at least two distinct breeds, Jakar-Sakten sheep and Sipsu sheep. Since Jakar and Sakten sheep have different morphological phenotypes, further analyses will be required to understand the genetic differences between these two sheep populations.

  WILEY-BLACKWELL PUBLISHING, INC, 2010, ANIMAL SCIENCE JOURNAL, 81(2) (2), 145 - 151, English

  [Refereed]

  Scientific journal


• Effect of SCD and SREBP genotypes on fatty acid composition in adipose tissue of Japanese Black cattle herds

  Hideki Ohsaki, Atsuko Tanaka, Shogo Hoashi, Shinji Sasazaki, Kenji Oyama, Masaaki Taniguchi, Fumio Mukai, Hideyuki Mannen

  Fatty acid composition of beef adipose tissue is one of important traits because high proportions of monounsaturated fatty acid are related to favorable beef flavor and tenderness. In this study, we investigated effects of genetic factors such as stearoyl-CoA desaturase (SCD) and sterol regulatory element binding protein (SREBP) on beef carcass traits including fatty acid composition using two cattle populations. Sire effect was significantly related to almost all traits except BMS, suggesting that the trait examined in this study is highly controlled by genetic factors. The effect of SCD genotype on fatty acid composition was detected remarkably in both cattle groups, especially on stearic and oleic acids. This result was consistent with our previous studies and suggests that SCD is associated with fatty acid composition. Unlike SCD genotyping, the effect of SREBP genotype was not identified in this study. Our results suggested that SCD genotype would contribute to improving beef quality in field populations. Further studies about the relationship among these factors will bring an insight into the molecular mechanism of fatty acid metabolism in cattle.

  WILEY-BLACKWELL PUBLISHING, INC, Jun. 2009, ANIMAL SCIENCE JOURNAL, 80(3) (3), 225 - 232, English

  [Refereed]

  Scientific journal


• Number of Calves Produced at Specified Age as a Measure of Reproductive Performance in Beef Cattle under Artificially-Inseminated Breeding Scheme

  Kenji Oyama, Shinya Fujiwara, Tomohiro Katsuta, Takeshi Honda, Fumio Mukai

  Reproductive abilities in beef cattle herds are receiving increased attention due to recent rises in production costs. To achieve more efficient management, a measure of fertility, namely the number of calves produced at k yr of age (NCPk), was developed and its genetic parameters were estimated from Japanese Black cows by restricted maximum likelihood procedures. The k examined were distributed from 2 to 10 yr of age and NCP(2) averaged 1.077 calves over 43,536 cows. The averages increased by approximately 0.9 calf with each additional 1 yr increment in k. Heritabilities of NCP(k) were estimated to be low ranging from 0.083 to 0.162, which seemingly suggested a difficulty of genetic improvement. However, large genetic variation and high accuracy were observed in predicted breeding values of NCP(k). For example, the breeding values of NCP(7) were predicted between -0.303 and +0.213 with average accuracy of 0.607 for cows with observations. Genetic correlations among different k were generally high and positive (0.474 to 0.995). The analyses showed that at least NCP(4) was required to maintain the genetic correlations of 0.8 or higher with subsequent NCP(k). Also NCP(5) maintained the genetic correlations of 0.9 or higher with subsequent NCP(k). The results suggested some possibilities for NCP(k) to be a selection criterion considering its genetic variation, high accuracy and consistency with subsequent performance.

  ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC, Feb. 2009, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 22(2) (2), 162 - 167, English

  [Refereed]

  Scientific journal


• Association between fatty acid compositions and genotypes of FABP4 and LXR-alpha in Japanese Black cattle

  Shogo Hoashi, Tomoko Hinenoya, Atsuko Tanaka, Hideki Ohsaki, Shinji Sasazaki, Masaaki Taniguchi, Kenji Oyama, Fumio Mukai, Hideyuki Mannen

  Background: Fatty acid composition has become an important trait in the beef industry in terms of beef flavor and decreasing the circulating concentration of LDL cholesterol. In this study, we examined the association between polymorphisms of six genes, adipocytes-type fatty acid binding protein (FABP4), liver X receptor alpha (LXR alpha), cytochrome b(5) (Cyt b(5)), long-chain acyl-CoA synthetase (ACSL) 1, ACSL4 and diacylglycerol acyltransferase 2 (DGAT2) and fatty acid composition. Results: Sequence comparisons revealed 14 single nucleotide polymorphisms in six genes. Four of them, I74V and V110M in FABP4 and G51E and V133I in LXR alpha, were nonsynonymous substitutions. The associations between the genotypes and fatty acid compositions were analyzed by using 234 Japanese Black cattle. The genotypes of FABP4 I74V and LXR alpha V133I were significantly associated with palmitoleic acids (C16: 1, P = 0.0086) and linoleic acid (C18:2, P = 0.0121) content in intramuscular fat, respectively. Conclusion: Our findings suggest that the two polymorphisms of FABP4 I74V and LXR alpha V133I might be genetic factors in part associated with palmitoleic acid (FABP4 I74V) and linoleic acid (LXR alpha V133I) composition in intramuscular fat of Japanese Black cattle, respectively. Especially, FABP4 I74V had highly significant effect (P < 0.01) on C16:1 proportion, indicating that the I/I homozygote exhibited 0.5% higher percentage than V/V homozygote.

  BIOMED CENTRAL LTD, Dec. 2008, BMC GENETICS, 9, 84, English

  [Refereed]

  Scientific journal


• Genetic analysis of residual feed intakes and other performance test traits of Japanese Black cattle from revised protocol

  Takeshi Okanishi, Masayuki Shojo, Tomohiro Katsuta, Kenji Oyama, Fumio Mukai

  The performance test protocol for Japanese Black cattle was revised in April 2002. This resulted in restriction of access to concentrate (based on body weight) and modification of the concentrate's ingredients. Genetic parameters of growth and feed utilization traits of the performance test were estimated using 1304 records using the revised protocol. Residual feed intakes (RFIs) as alternative indicators for feed utilization efficiency were included. (Co)variance components were estimated by EM-REML. Heritabilities for growth traits were between 0.26 and 0.47. Heritabilities for feed intakes and conversions ranged from 0.25 to 0.37 and from 0.03 to 0.29, respectively. Genetic variances and heritabilities were lower for the revised protocol. Highly positive genetic correlations of daily gain (DG) with feed intakes indicated selection on DG is expected to increase feed intake. Selection on feed conversion may lead to higher DG. The heritability estimates for RFIs ranged from 0.10 to 0.33 and were generally higher than corresponding estimates for feed conversion ratios. RFI of TDN showed positive genetic correlations with all feed intakes. The reduction of feed intakes could be expected through selection on the RFI without changing body size. RFIs were considered to be alternative indicators to improve feed utilization efficiency under the new performance test.

  WILEY-BLACKWELL, Jun. 2008, ANIMAL SCIENCE JOURNAL, 79(3) (3), 291 - 296, English

  [Refereed]

  Scientific journal


• Genotype of bovine sterol regulatory element binding protein-1 (SREBP-1) is associated with fatty acid composition in Japanese Black cattle

  Shogo Hoashi, Nobuhisa Ashida, Hideki Ohsaki, Takeshi Utsugi, Shinji Sasazaki, Masaaki Taniguchi, Kenji Oyama, Fumio Mukai, Hideyuki Mannen

  To investigate genetic factors that affect fatty acid composition in beef carcass, we previously investigated genetic profiles of stearoyl-CoA desaturase (SCD) and their effect on fatty acid composition in fat tissue of cattle. It has been known that sterol regulatory element binding protein (SREBP) is a transcription factor that regulates gene expression levels of SCD and other genes relevant to lipid and fatty acid metabolism in tissue. Therefore, we determined the full-length sequence of bovine SREBP-1 cDNA and then surveyed polymorphisms in whole exons and introns in the bovine genome. Large 84-bp insertion (long type: L) and deletion (short type: S) were found in intron 5 of bovine SREBP-1 in Japanese Black cattle, although there was no notable mutation in exon regions. The associations between the SREBP-1 genotypes and fatty acid compositions/fat melting points were analyzed by using genomic DNA with carcass trait information from 606 Japanese Black cattle. The S type contributed to 1.3% higher monounsaturated fatty acid (MUFA) proportion and 1.6 degrees C lower melting point in intramuscular fat. Genotyping of bovine SREBP-1 is considered to reflect a genetic variation which is associated with physiologic characteristics of fat tissue in Japanese black cattle.

  SPRINGER, Dec. 2007, MAMMALIAN GENOME, 18(12) (12), 880 - 886, English

  [Refereed]

  Scientific journal


• Effect of sire mating patterns on future genetic merit and inbreeding in a closed beef cattle population

  K. Oyama, M. Nojima, M. Shojo, M. Fukushima, K. Anada, F. Mukai

  Alternative breeding strategies were simulated based on the population structure of the Tajima strain of Japanese Black cattle. An analysis of the population structure revealed that some sires up to 20 years of age have been used in Tajima. In addition, 95% of newborn calves were the progeny of only 20 sires, and their mating frequencies were significantly skewed. The current average inbreeding coefficient and founder genome equivalents of the strain were estimated to be 0.199 and 2.25, respectively. Average inbreeding coefficient is expected to reach 0.394 within 27 years. Thus, different breeding strategies were assessed for their effect on the level of inbreeding and average genetic merit. We compared strategies that (1) halve the sire service period, (2) double the number of mating sires and (3) lower the skewed sire mating frequency and optimize the frequency for weighted genetic merit and diversity. Reducing the service period yielded a 7.0-12.0% reduction in the rate of inbreeding while maintaining almost the same genetic gain. Increasing the number of sires resulted in a 19.3-21.3% reduction in inbreeding with a corresponding 1.6-8.4% reduction in gain. The rates of inbreeding from the optimized strategies decreased as the weight on genetic diversity increased. However, a strategy that emphasized only genetic gain yielded lower gain than other strategies because the strategy allowed only one sire to mate, resulting in reduced genetic variance and low accuracy of genetic evaluation. In contrast, a strategy with no emphasis on genetic gain when determining mating frequency resulted in reductions of 16.0% and 63.2% in genetic gain and inbreeding, respectively. The strategies examined here are easily applicable and can be expected to reduce immediate loss of genetic diversity.

  BLACKWELL PUBLISHING, Apr. 2007, JOURNAL OF ANIMAL BREEDING AND GENETICS, 124(2) (2), 73 - 80, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Genetic analysis of calf market weight and carcass traits in Japanese Black cattle

  M. Shojo, T. Okanishi, K. Anada, K. Oyama, F. Mukai

  Heritabilities of and genetic correlations between additive direct and maternal genetic effects for calf market weight, and additive direct genetic effects for carcass traits, were estimated for Japanese Black cattle by REML procedures under 2-trait animal models. Data were collected from calf and carcass markets in Hyogo and Tottori prefectures and analyzed separately by prefecture. Calf market weight was measured on 42,745 and 23,566 calves in Hyogo and Tottori, respectively. Only the fattening animals with calf market weight were extracted from the carcass database and used for estimation. The carcass traits analyzed were carcass weight, ribeye area, rib thickness, subcutaneous fat thickness, yield estimate, beef marbling score, and 4 meat characters (color, brightness, firmness, and texture). Direct and maternal heritabilities for calf market weight were estimated to be 0.22 and 0.07 in Hyogo, and 0.37 and 0.15 in Tottori, respectively. The estimates of heritabilities for carcass traits were moderate to high in both prefectures. The estimates of direct-maternal genetic correlations for calf market weight were positive (0.17) in Hyogo and negative (-0.63) in Tottori. The direct effect for calf market weight was positively correlated with the direct effect for carcass weight (0.87 and 0.56 in Hyogo and Tottori, respectively) but negatively correlated with the direct effect for beef marbling score (-0.10 in both prefectures). The estimates of genetic correlations between the maternal effect for calf market weight and the direct effects for carcass traits varied from -0.13 to 0.34 in Hyogo and from -0.14 to 0.15 in Tottori. Because direct and maternal genetic effects for early growth traits can be evaluated from calf market weight data in the production system of Japanese Black cattle, this information should be incorporated into selection and mating schemes of the breed.

  AMER SOC ANIMAL SCIENCE, Oct. 2006, JOURNAL OF ANIMAL SCIENCE, 84(10) (10), 2617 - 2622, English

  [Refereed]

  Scientific journal


• Transcriptional profiling of skeletal muscle tissue from two breeds of cattle

  YH Wang, KA Byrne, A Reverter, GS Harper, M Taniguchi, SM McWilliam, H Mannen, K Oyama, SA Lehnert

  We used a 9.6 K cattle muscle/fat cDNA microarray to study gene expression differences between the longuissimus dorsi (LD) muscle of Japanese Black (JB) and Holstein (HOL) cattle. JB cattle exhibit an unusual ability to accumulate intramuscular adipose tissue with fat melting points lower than that in other breeds. The LD biopsies from three JB (Tajima strain) and three HOL animals were used in this breed comparison. Seventeen genes were identified as preferentially expressed in LD samples from JB and seven genes were found to be expressed more highly in HOL. The expression of six selected differentially expressed genes was confirmed by quantitative real-time PCR. The genes more highly expressed in JB are associated with unsaturated fatty acid synthesis, fat deposition, and the thyroid hormone pathway. These results are consistent with the increased amounts and proportions of monounsaturated fatty acids observed in the muscle of JB animals. By discovering as yet uncharacterized genes that are differentially regulated in this comparison, the work may lead us to a better understanding of the regulatory pathways involved in the development of intramuscular adipose tissue.

  SPRINGER, Mar. 2005, MAMMALIAN GENOME, 16(3) (3), 201 - 210, English

  [Refereed]

  Scientific journal


• Transcriptional profiling of skeletal muscle tissue from two breeds of cattle

  YH Wang, KA Byrne, A Reverter, GS Harper, M Taniguchi, SM McWilliam, H Mannen, K Oyama, SA Lehnert

  We used a 9.6 K cattle muscle/fat cDNA microarray to study gene expression differences between the longuissimus dorsi (LD) muscle of Japanese Black (JB) and Holstein (HOL) cattle. JB cattle exhibit an unusual ability to accumulate intramuscular adipose tissue with fat melting points lower than that in other breeds. The LD biopsies from three JB (Tajima strain) and three HOL animals were used in this breed comparison. Seventeen genes were identified as preferentially expressed in LD samples from JB and seven genes were found to be expressed more highly in HOL. The expression of six selected differentially expressed genes was confirmed by quantitative real-time PCR. The genes more highly expressed in JB are associated with unsaturated fatty acid synthesis, fat deposition, and the thyroid hormone pathway. These results are consistent with the increased amounts and proportions of monounsaturated fatty acids observed in the muscle of JB animals. By discovering as yet uncharacterized genes that are differentially regulated in this comparison, the work may lead us to a better understanding of the regulatory pathways involved in the development of intramuscular adipose tissue.

  SPRINGER, Mar. 2005, MAMMALIAN GENOME, 16(3) (3), 201 - 210, English

  Scientific journal


• 但馬牛集団のジーンドロッピング法による系統分類の試み

  福島 護之, 坂瀬 充洋, 野田 昌信, 武田 和士, 上野 悟, 本多 健, 大山 憲二, 向井 文雄

  但馬牛における始祖個体遺伝子の消長をジーンドロッピング法で調べ，その結果を基に普遍的な系統分類の方法を検討した．1 現存牛の血統を可能な限り遡り出現した牛のうち名号，生年月日が明らかで，産地が兵庫県内の牛を始祖個体としたところ，条件を全て満たす個体は1,461頭(累計遺伝的寄与率0.773)となった．系統分類のための始祖個体は，Pr(lost)の小さい100頭に限定した．2 始祖個体の生産地は城崎郡14頭(累積寄与率0.0314)，出石郡2頭(累積寄与率0.0002)，美方郡82頭(累積寄与率0.637)と養父郡2頭であったが，当時の郡境からは養父郡の2頭は美方郡に分類されていると考えられ，美方郡産が84頭にのぼり寄与率が高かった．3 現存個体27,138頭に対する始祖個体からの遺伝的寄与率を計算し，その結果を基に主成分分析を行った．第3主成分までで約85％を説明でき，現在個体の頭数割合が3.7～25.9％の8グループに分類できた．4 各グループ内及びグループ間の平均血縁係数から，G1～G4とG8ではグループ間の平均血縁係数がグループ内の平均血縁係数よりも高いグループが存在し，近年は系統外の種雄牛との交配が増加したことに起因すると考えられた．G5～G7では，グループ内の平均血縁係数が最も高く，依然，系統内交配が主流であると考えられた．5 グループ毎の近交係数は，16,143頭の結果からG1～G4では13.0～17.8％と低く，G5～G8で18.7～23.2％と高い傾向にあった．6 グループ毎の枝肉重量の育種価の平均は-8.1～-2.7とグループ間でばらついていた．BMS育種価の平均に関しても，0.63～1.00とばらついていたが，各グループともに最大値をみると1.477～2.137と能力が高い個体から含まれていた．以上の結果から，ジーンドロッピング法により選定した100頭の始祖個体を元にした系統分類法によって現在の但馬牛を分類することが可能であり，頭数や産肉能力から見ても遺伝的多様性を保持しつつ，今後の改良が可能であることが明らかとなった．

  兵庫県立農林水産技術総合センター, 2005, 兵庫県立農林水産技術総合センター研究報告〔畜産編〕, 41 16-21(41) (41), 16 - 21, Japanese

  [Refereed]

  Scientific journal


• Trial for detecting carriers with major genes in a selected layer line

  Ayako Ochiai, Takafumi Ishida, Kenji Oyama, Fumio Mukai

  Production traits of a Rhode Island Red layer line were analyzed to detect carrier animals with major genes that influenced a particular trait by applying the major gene index (MGI) approach. This method was used to examine the deviation of predicted breeding values between parents and offspring. Advantages of this method include its simplicity and time-reducing, cost-saving benefits compared with any other statistical approach for screening major genes. The layer line had been selected based on the desired gain index for 6 years at the National Livestock Breeding Center, Okazaki station. The line consisted of 125 sires and 2986 dams. Two economic traits as breeding objectives were studied - age at sexual maturity (ASM, days) and egg production efficiency (EP, %). The MGI detected nine sires and 23 dams as carriers with major genes for ASM, and five sires and 26 dams for EP. They were identified as important breeding stock, and pedigree information provided candidates possessing major genes with favorable effect at the founding of the selected layer line. It seems likely that the simple approach of the MGI could be a useful preliminary method for detecting carrier animals with major genes before applying molecular techniques on a sampled population to identify in more detail the existence of major genes and their carriers.

  Blackwell Publishing, 2005, Animal Science Journal, 76(3) (3), 195 - 201, English

  [Refereed]

  Scientific journal


• Transcriptional profiling of muscle tissue in growing Japanese Black cattle to identify genes involved with the development of intramuscular fat

  YH Wang, A Reverter, H Mannen, M Taniguchi, GS Harper, K Oyama, KA Byrne, A Oka, S Tsuji, SA Lehnert

  Japanese Black cattle are characterised by a unique ability to deposit intramuscular fat with lower melting temperature. In this study, 3 consecutive biopsies from Longissimus muscle tissue were taken and RNA isolated from 3 Japanese Black (Tajima strain) and 3 Holstein animals at age 11 - 20 months. The gene expression changes in these samples were analysed using a bovine fat/muscle cDNA microarray. A mixed-ANOVA model was fitted to the intensity signals. A total of 335 (4.8%) array elements were identified as differentially expressed genes in this breed x time comparison study. Genes preferentially expressed in Japanese Black are associated with mono-unsaturated fatty acid synthesis, fat deposition, adipogenesis development and muscle regulation, while examples of genes preferentially expressed in Holstein come from functional classes involved in connective tissue and skeletal muscle development. The gene expression differences detected between the Longissimus muscle of the 2 breeds give important clues to the molecular basis for the unique features of the Japanese Black breed, such as the onset and rate of adipose tissue development, metabolic differences, and signalling pathways involved in converting carbohydrate to lipid during lipogenesis. These findings will impact on industry management strategies designed to manipulate intramuscular adipose development at different development stages to gain maximum return for beef products.

  C S I R O PUBLISHING, 2005, AUSTRALIAN JOURNAL OF EXPERIMENTAL AGRICULTURE, 45(7-8) (7-8), 809 - 820, English

  Scientific journal


• Genetic base of Indian potato selections as revealed by pedigree analysis

  J Gopal, K Oyama

  Pedigree analysis of the 77 advanced Indian potato selections showed that their origin could be traced to only 49 ancestors out of which 29 were exotic, which accounted for 69.52% of the total genomic constitution and maximum contribution (40.65%) was by 10 ancestors from U.K. Breeders' tendency was to involve adapted advanced clones as immediate parents. Coefficient of relationship between pair of selections ranged from 0 to 0.68. The success of the parents used was not related to region-specific choice of parents. The findings are discussed in the context of "genetic uniformity" and the relevance of having separate breeding programmes for the three major areas of potato cultivation in India. Attempt has also been made to give some directions for choosing selections for use as parents and release as cultivars based on their coefficient of relationship so as to broaden the genetic base of the future potato cultivars.

  SPRINGER, Jan. 2005, EUPHYTICA, 142(1-2) (1-2), 23 - 31, English

  [Refereed]

  Scientific journal


• Genealogical relationship between pedigree and microsatellite information and analysis of genetic structure of a highly inbred Japanese black cattle strain

  S Sasazaki, T Honda, M Fukushima, K Oyama, H Mannen, F Mukai, S Tsuji

  Japanese Black cattle of Hyogo prefecture (Tajima strain) are famous for its ability to produce high-quality meat and have been maintained as a closed system for more than 80 years. In order to assess the usefulness of microsatellite markers in closed cattle populations, and evaluate the genetic structure of the Tajima strain, we analyzed representative dams of the Tajima strain comprised of the substrains Nakadoi and Kinosaki. Genetic variability analyses indicated-low genetic diversity in the Tajima strain. In addition, a recent genetic bottleneck, which could be accounted for by the high level of inbreeding, was detected in both substrains. In phylogenetic analyses, relationship coefficients and genetic distances between individuals were calculated using pedigree and microsatellite information. Two phylogenetic trees were constructed from microsatellite and pedigree information using the UPGMA method. Both trees illustrated that most individuals were distinguished clearly on the basis of the two substrains, although in the microsatellite tree some individuals appeared in clusters of different substrains. Comparing the two phylogenetic trees revealed good consistency between the microsatellite analysis tree and the pedigree information. The correlation coefficient between genetic distances derived from microsatellite and pedigree information was 0.686 with a high significance level (p<0.001). These results indicated that microsatellite information may provide data substantially equivalent to pedigree information even in unusually inbred herds of cattle, and suggested that microsatellite markers may be useful in revealing genetic structure without accurate or complete pedigree information.

  ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOCIETIES, Oct. 2004, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 17(10) (10), 1355 - 1359, English

  Scientific journal


• Trace of native cattle in Japanese Holstein assessed by mitochondrial DNA sequence polymorphism

  S Tsuji, H Mannen, F Mukai, M Shojo, K Oyama, T Kojima, C Kano, Y Kinoshita, E Yamaguchi

  On the basis of sequence variation in the displacement loop region of mtDNA, 588 Japanese and North American Holstein cows were classified into 5 mitochondrial haplotypes, which were found in Japanese Black cattle. One of the haplotypes (named type 1), which was present at the highest frequency in Japanese Black cattle, was not observed in either European or African cattle. This haplotype is characterized by 2 single-nucleotide polymorphisms. One is called the type B polymorphism, and it refers to a base change from T to C at nucleotide 16042 of the mitochondrial genome (T160042C). The other is called the type I polymorphism, and it refers to the base change as G16093A. The proportion of the Japanese Holstein population with both polymorphisms was 18.3%, whereas none of the North American cows had this genotype. Because the mitochondrial types were inherited maternally, it is clear that a considerable number of Japanese Holstein cows are descended from native Japanese cattle. Polymorphisms B and I accounted for no variance in the estimated breeding value for milk production among cows from the Hyogo herd (582 cows) or the Chiba region herd (758 cows). This result suggests that most autosomal genes of native animals have been successively replaced by those of pure Holstein after grading up of over 15 generations, even though resulting animals have native animal-oriented mitochondrial types and may still have some number of the native autosomal genes.

  AMER DAIRY SCIENCE ASSOC, Sep. 2004, JOURNAL OF DAIRY SCIENCE, 87(9) (9), 3071 - 3075, English

  Scientific journal


• Differences in stearoyl-CoA desaturase mRNA levels between Japanese Black and Holstein cattle

  M Taniguchi, H Mannen, K Oyama, Y Shimakura, A Oka, H Watanabe, T Kojima, A Komatsu, GS Harper, S Tsuji

  The effect of cattle breed on stearoyl-CoA desaturase (SCD) gene expression was investigated in this study. Detailed comparisons of SCD mRNA level were made among three steers each of Japanese Black, Holstein and their crossbreed which were age-matched had been fed the same diet and were sampled by biopsy of the longissimus dorsi (LD) muscle and subcutaneous fat. The levels of SCD mRNA were measured in samples of muscle and subcutaneous fat. The levels of SCD mRNA demonstrated a breed effect in each tissue, though the relative expression was higher (P < 0.05) in subcutaneous fat. The ratio of SCD mRNA to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA for Japanese Black, crossbreed and Holstein were 132.1 +/- 34.1, 73.5 +/- 22.7 and 39.5 +/- 12.9, respectively, and significant (P<0.05) differences existed between Japanese Black and Holstein cattle. Japanese Black subcutaneous fat had consistently higher (P<0.05) monounsaturated fatty acids (MUFA) percentage than Holstein subcutaneous fat. These results suggest that differences in SCD gene expression may contribute to the fatty acid compositional differences seen between subcutaneous adipose tissue of Japanese Black cattle and Holstein. (C) 2003 Elsevier B.V. All rights reserved.

  ELSEVIER SCIENCE BV, May 2004, LIVESTOCK PRODUCTION SCIENCE, 87(2-3) (2-3), 215 - 220, English

  Scientific journal


• Genotype of stearoyl-CoA desaturase is associated with fatty acid composition in Japanese Black cattle

  M Taniguchi, T Utsugi, K Oyama, H Mannen, M Kobayashi, Y Tanabe, A Ogino, S Tsuji

  To investigate the genetic factors that affect fatty acid composition of beef, we compared the full-length bovine stearoyl-CoA desaturase (SCD) cDNA from 20 Japanese Black steers. Two types of the SCD gene with single nucleotide polymorphisms (SNPs) were observed in the ORF of SCD cDNA, in which an amino acid replacement from valine (type V) to alanine (type A) was predicted. We developed a method for genotyping these two SCD genes based on PCR-RFLP. We have classified 1003 Japanese Black carcasses into three genotypes, VV, VA, and AA, and compared fatty acid composition among them. The SCD type A gene contributed to higher MUFA percentage and lower melting point in intramuscular fat. The SCD genotype was not the only genetic factor contributing to fatty acid composition of Japanese Black steers, but the SCD genotype was considered one of the causes of genetic variation in fatty acid composition of Japanese Black steers. Transcription factors such as sterol regulatory element binding protein-1c (SREBP-1c) may account for the remaining part of the genetic variation in fatty acid composition.

  SPRINGER-VERLAG, Feb. 2004, MAMMALIAN GENOME, 15(2) (2), 142 - 148, English

  Scientific journal


• An association study using AFLP markers and application to a beef cattle breeding population

  S Tsuji, K Itoh, S Sasazaki, H Mannen, K Oyama, M Shojo, F Mukai

  Using amplified fragment length polymorphism (AFLP) fingerprinting, selective genotyping was performed to determine if this method was effective for selecting superior breeding stock. Forty-eight cows with extreme genetic merit for beef marbling score (BMS) were selected from a population of Japanese Black cattle (n = 4462), including 25 with the highest for predicted breeding value (PBV) and 23 with the lowest. Sixteen AFLP fragments were selected for further analysis based on fragment frequency differences between the high and low groups. A linear discriminant analysis using these AFLP fragments was applied in order to derive a discriminant function that classified the cows into high and low groups. Seven of the 16 fragments were included in the resulting function and the discriminant scores (general genetic values, GGV) of the 48 cows were calculated using the function. These cows were clearly separated into high and low groups by GGV with a correlation ratio of 0.91 (discriminative error of 2.1%). The same function was then applied to 121 additional cows that were randomly selected from the original population. A significant regression coefficient of GGV on BMS-PBV (R-2 = 0.45) was obtained, which indicates that the GGV can be used as a selection criterion for BMS in this population. These results suggest that AFLP fingerprinting can be used for animal breeding without identifying the underlying genes affecting the trait of interest.

  BLACKWELL PUBLISHING LTD, Feb. 2004, ANIMAL GENETICS, 35(1) (1), 40 - 43, English

  Scientific journal


• Trace of native cattle in Japanese Holstein assessed by mitochondrial DNA sequence polymorphism

  S. Tsuji, H. Mannen, F. Mukai, M. Shojo, K. Oyama, T. Kojima, C. Kano, Y. Kinoshita, E. Yamaguchi

  On the basis of sequence variation in the displacement loop region of mtDNA, 588 Japanese and North American Holstein cows were classified into 5 mitochondrial haplotypes, which were found in Japanese Black cattle. One of the haplotypes (named type 1), which was present at the highest frequency in Japanese Black cattle, was not observed in either European or African cattle. This haplotype is characterized by 2 single-nucleotide polymorphisms. One is called the type B polymorphism, and it refers to a base change from T to C at nucleotide 16042 of the mitochondrial genome (T160042C). The other is called the type I polymorphism, and it refers to the base change as G16093A. The proportion of the Japanese Holstein population with both polymorphisms was 18.3%, whereas none of the North American cows had this genotype. Because the mitochondrial types were inherited maternally, it is clear that a considerable number of Japanese Holstein cows are descended from native Japanese cattle. Polymorphisms B and I accounted for no variance in the estimated breeding value for milk production among cows from the Hyogo herd (582 cows) or the Chiba region herd (758 cows). This result suggests that most autosomal genes of native animals have been successively replaced by those of pure Holstein after grading up of over 15 generations, even though resulting animals have native animal-oriented mitochondrial types and may still have some number of the native autosomal genes.

  American Dairy Science Association, 2004, Journal of Dairy Science, 87(9) (9), 3071 - 3075, English

  Scientific journal


• Selective genotyping for marker assisted selection in Japanese Black cattle.

  SASAZAKI S, ITOH K, URATSUJI H, MANNEN Hideyuki, OYAMA Kenji, SHOJO Masayuki, MUKAI Fumio, TSUJI S

  2004, Proc. 29th Int. Conf. on Anim. Genet., p.138, English

  International conference proceedings


• Comparisons between Japanese Black and Holstein cattle using gene expression profiling.

  Wang Y H, OYAMA Kenji, Reverter-Gomez A, TANIGUCHI M, Byrne K A, Harper G S, MANNEN Hideyuki, TSUJI S, LEHNERT S A

  2004, Proc. 29th Int. Conf. on Anim. Genet., p.70, English

  International conference proceedings


• 但馬牛集団の血縁係数による系統分類の試み

  福島 護之, 大山 憲二, 坂瀬 充洋, 野田 昌伸, 武田 和士, 有吉 哲志, 向井 文雄

  血縁係数を利用したクラスター分析により但馬牛集団の系統分類を試み、現存集団の遺伝的構成を把握し、系統の再構築の可能性を検討した。とくに、今回の分析で規模が小さな集団の増頭を目的として、仮想集団を作出した場合の系統分類についても検討した。1.供用中集団のデンドログラムを血縁係数0.10で切断し、7グループに分類した。2.各グループ内及びグループ間の平均血縁係数は、グループ1、2、3と5では全て0.3以上であった。グループ4も概ね0.3で上記グループとの血縁が高かった。グループ6と7が、他グループと異なりグループ間の血縁係数が低かった。3.各グループの育種価評価値の平均値を比較するとグループ毎に産肉あるいは種牛能力の特徴が明らかになった。4.600頭の仮想雌牛群と待機雄牛の後代を加え系統分類を実施した。その結果、中土井系繁殖雌牛に城崎系種雄牛を交配するシミュレーション集団1では、雌雄が逆の系統に属する交配集団2に比較して高率(1.7%vs16.0%)に城崎系であるグループが作出された。集団1では、種雄牛による差が大きく、奥秀、城富、城和、勘高や大勘で城崎系産子を生産する割合が高かったが、光城波は亜系の新たなグループを形成した。集団2では、城崎系を生産する母牛が6頭程度に限られ、中土井系の種雄牛を交配することで城崎系の産子を生産する可能性は低かった。

  兵庫県立農林水産技術総合センター, 2003, 兵庫県立農林水産技術センター研究報告［畜産編］, 39:16-21(39) (39), 16 - 21, Japanese

  Scientific journal


• ウシ体脂肪の脂肪酸組成に影響する遺伝的要因

  谷口 雅章, 万年 英之, 大山 憲二, 宇津木 健司, 辻 荘一

  日本動物遺伝育種学会, 2003, 動物遺伝育種研究, 30:17-20.(2) (2), 17 - 20, Japanese

  Scientific journal


• Identification of mitochondrial DNA substitutions related to meat quality in Japanese Black cattle

  H Mannen, M Morimoto, K Oyama, F Mukai, S Tsuji

  Complete sequences of mitochondrial (mt) genomes of eight Japanese Black cattle were determined to investigate the relationships between mt deoxyribonucleic acid (DNA) displacement loop (D-loop) types and other mtDNA regions and to identify the variation in the coding region that may influence the economic traits. The survey of mitochondrial sequences in the encoding region revealed 14 substitutions including six antonymous substitutions and one in 16S ribosomal ribonucleic acid (rRNA). Three methods of polymorphic DNA analyses (polymerase chain reaction [PCR]-restriction fragment length polymorphism [RFLP], mismatch PCR-RFLP, PCR-single-strand conformation polymorphism [SSCP]) were performed on these seven candidate substitutions (base pair [bp] 2,232, 12,158, 12,908, 13,310, 14,122, 14,140, and 14,565) for 202 Japanese Black cattle. The substitution of by 13,310 was observed in all samples, but not in the reference sequence, indicating that this is a minor substitution or a sequencing mistake in the reference sequence. The substitutions at by 14,122, 14,140, and 14,565 were observed in only a few samples, suggesting that these were also minor substitutions. The substitutions at by 2,232 (16S rRNA), 12,158, and 12,908 (reduced nicotinamide adenine dinucleotide-ubiquinone oxidoreductase chain-5) were closely related to mitochondrial D-loop types that have previously been related to differences in the carcass traits of Japanese Black cattle. Evaluation of the effects on six carcass traits with mixed model procedures suggests that the by 2,232 substitution affects longissimus muscle area and beef marbling score. The substitution at by 2,232 is a strong candidate for the mitochondrial effect on meat quality.

  AMER SOC ANIMAL SCIENCE, Jan. 2003, JOURNAL OF ANIMAL SCIENCE, 81(1) (1), 68 - 73, English

  Scientific journal


• Genetic parameters for reproductive performances of breeding cows and carcass traits of fattening animals in Japanese Black (Wagyu) cattle.

  OYAMA Kenji, KATSUTA T, ANADA K, MUKAI Fumio

  2003, Animal Genetics, 未記入, English

  Scientific journal


• Differences in Stearoyl-CoA Desaturase mRNA levels between Japanese Black and Holstein cattle.

  TANIGUCHI M, MANNEN Hideyuki, OYAMA Kenji, OKA A, WATANABE H, KOJIMA T, KOMATSU M, HARPER S G, TSUJI S

  2003, Livest. Prod. Sci., 未記入, 215 - 220, English

  Scientific journal


• Heritability and repeatability estimates for reproductive traits of Japanese black cows

  K Oyama, T Katsuta, K Anada, F Mukai

  Reproductive data collected from more than 20,000 Japanese Black cows of Hyogo and Shimane Prefectures were analyzed. Averages of age at first calving, gestation length, days open and calving interval were 25.1 mo, 289 d, 112 d and 401 d, respectively. Variance components were obtained by REML procedure and the heritability estimate of age at first calving was 0.22. In gestation length the heritability estimate was 0.40 and no permanent environmental effect was estimated. Estimated variance components of calving interval were similar to those of days open and the heritability and repeatability of calving interval were 0.0 and 0.09, respectively. Random farm effects accounted for approximately 10% of phenotypic variations in all traits. Genetic and farm correlations between age at first calving and calving interval were 0.27 and 0.39, respectively. It was found that temporary environment was an important source of variation for calving intervals of Japanese Black.

  ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOCIETIES, Dec. 2002, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 15(12) (12), 1680 - 1685, English

  [Refereed]

  Scientific journal


• Genetic Improvement of Japanese Black Cattle Based on Predicted Breeding Values

  OYAMA Kenji

  Genetic relationships among carcass traits and between reproductive or growth traits and carcass traits were estimated for Japanese Black using REML procedure. Among carcass traits a low but favorable genetic correlation (-0.04) was obtained between two carcass traits relating to fat deposition; beef marbling score and subcutaneous fat thickness, suggesting that these traits could be improved simultaneously. Beef marbling score had a weak (-0.01) and an undesirable (0.25) relationships with daily gain of young bulls at performance testing and calving interval of breeding females, respectively. These estimates indicated truncation selection by daily gain would remove superior young bulls in marbling from selection candidates and selection by marbling would lead calving interval longer. Hence, selection criteria, such as estimated or predicted breeding values, of beef marbling and calving interval should be introduced into appropriate selection stages of breeding programs. However a caution will be needed for rapid increase in inbreeding because very low heritability (0.05) was estimated for calving interval. To avoid the problem a simulation study, which examines the effectiveness of a simple genetic algorithm to optimize mating design, was conducted. The mating designed by genetic algorithm succeeded to reduce 8.1% to 14.8% of inbreeding level for various heritabilities compared with random mating after nine generations of selection and mating. The possibility of the algorithm to maintain genetic diversity of Japanese Black was demonstrated.

  Kobe University, Feb. 2002, The science reports of Faculty of Agriculture, Kobe University, 26, 49 - 54, Japanese


• Determination of the Optimum Mating Design with Constraints on Inbreeding Level and Mating Frequency of Sires via a Simple Genetic Algorithm

  OYAMA Kenji, MUKAI Fumio

  Using predicted breeding values (PBV) of an actual beef cattle population, application of a simple genetic algorithm (GA) was done to design the optimum mating, with constraints on inbreeding coefficient of each progeny, inbreeding level of the next generation, and mating frequency of sires. As the breeding stock, 15 sires and 400 dams were selected according to PBV of judging score for breeding females (JS-Data) or beef carcass marbling score (BMS-Data). Aggregate genetic merit (AGM) of eight traits (judging score, two reproductive traits, and five carcass traits) weighing differently was regarded as the breeding objective. When appropriate operator values were used, GA found satisfactory mating designs under pre-determined constraints. The losses in gains of AGM by imposing the constraint of 0.30 on mating frequency were only 31.3% in JS-Data and 35.6% in BMS-Data compared with the mating designs without constraints. Genetic gain of beef marbling score was 28.3% higher in the evaluation from BMSData than JS-Data. Efficiencies of GA to design mating were demonstrated.

  Japanese Society of Animal Science, Apr. 1998, Nihon Chikusan Gakkaiho, 69(4) (4), 333 - 340, English


• Effect of mitochondrial DNA variation on carcass traits of Japanese Black cattle

  H Mannen, T Kojima, K Oyama, F Mukai, T Ishida, S Tsuji

  Japanese Black fattening steers were used to examine relationships between carcass traits and mitochondria displacement loop (D-loop) variations. The D-loop region of Japanese Black cattle was sequenced and revealed 26 mitochondrial haplotypes defined by 25 polymorphic sites. The haplotypes were classified into five mitochondrial types (type 1 to 5) using the unweighted pair-group method with arithmetic means. Carcass weight, longissimus muscle area (LMA), rib thickness, subcutaneous fat thickness, yield estimate, and beef marbling score (BMS) were compared among five mitochondrial types with BLUP procedures. Significant differences between mitochondria types were detected for LMA and BMS. Difference (P <.05) was observed between mitochondrial types 2 and 4 for LMA. There was a highly significant difference (P <.01) in EMS between types 2 and 4. Difference (P <.05) was also found between types 1 and 4 on BMS. These results suggest that cytoplasmic genetic effects are important sources of variation for carcass traits in Japanese Black cattle.

  AMER SOC ANIMAL SCIENCE, Jan. 1998, JOURNAL OF ANIMAL SCIENCE, 76(1) (1), 36 - 41, English

  [Refereed]

  Scientific journal


• A Nature of Genetic Parameter Values Calculated on the Assumptions of Linearity and Normality for Ratio Traits in Japanese Black Cattle

  IWAISAKI Hiroaki, OYAMA Kenji, MUKAI Fumio, SHOJO Masayuki

  For a sub-population of the Japanese Black cattle, values of genetic parameters forcertain ratio traits and their components (numerator and denominator traits) were estimatedunder the assumptions of linearity and normality. Then, assuming individual selection on phenotypic values of the ratio traits, the nature and validity of the parameter values for the ratio traits was examined for predicting genetic changes for the ratio and component traits. Ratio traits studied were TDN efficiency (TDNE) defined as the ratio of weight gain (WG) to TDN intake (TDNI), and roughage intake ratio (RIR) expressed as roughage intake (RI) divided by total feed intake (TFI). The estimation of (co)variances was conducted by the restricted maximum likelihood procedure with a reduced animal model, using records of 662 bull calves performance-tested. In this study, the parameter estimates for the component traits were assumed to equal the current true values. For the TDNE-based selection, with the assumptions of linearity and normality, genetic changes in upward and downward directions were predicted for WG and TDNI, respectively, which corresponded to the directions of response expected truly. However, the amount of the change was overestimated for WG and underestimated for TDNI. For the selection on RIR, the changes expected truly in the component traits were both in downward directions, but the prediction on the assumptions yielded positive and negative values of response expected for RI and TFI, respectively. The amounts of the changes predicted utilizing information of the parameters defined based on a Taylor-series expansion of ratio also did not agree with those expected truly. These results indicate a possibility that the information of parameters obtained based on animal models treating the ratio traits as a single, normally-distributed trait is not appropriate for determing genetic changes for the ratio and component traits expected truly fromthe ratio traits-based selection. It would be worth making a comment that such changes in the negative direction for both RI and TFI as expected from the RIR-based selection may be undesirable for optimized food intake capacity.

  Japanese Society of Animal Science, Nov. 1996, Nihon Chikusan Gakkaiho, 67(11) (11), 949 - 955, English


• Prediction of Direct Response and Its Correlated Response Under Selection on Predicted Breeding Values

  OYAMA Kenji, MUKAI Fumio

  Three methods of predicting direct and correlated responses were compared. Selection based on predicted breeding values, in which accuracies of selection varied for each candidate, was performed using computer simulation. The three methods were 1) average accuracy (AA), in which accuracies of selection in the standard equations were replaced with average accuracies of predicted breeding values; 2) group accuracy (GA), in which candidates for selection were classified according to their accuracies and weighted genetic gains were calculated from each class; and 3) selection differential of predicted breeding values (SP). All three methods were comparable in predicting direct response. However AA showed severe underpredictions in correlated response. Generally, SP was more effective than GA for predicting correlated response where correlation between predicted breeding values and their accuracies did not exist.

  Japanese Society of Animal Science, Nov. 1996, Nihon Chikusan Gakkaiho, 67(11) (11), 930 - 940, English


• A Comparison of Actual and Expected Selection Responses from Selection Indices for Desired Gains in Layer Chicken Lines

  MUKAI Fumio, OYAMA Kenji, TSUJI Soichi, MIYATA Toru

  In Okazaki station of National Livestock Improvement Center, selection index for desired gains based on the assumed parameters has been used to improve layer chicken. The same indices were applied on three lines from 1989 to 1994. Pre-specified restrictions as objective were no change on egg weight at 252 days of age (EW) and body weight at 256 days of age (BW), 5% increase of egg production efficiency from first egg to 280 days of age in all lines, and more five days reduction of age at first egg (SM) was aimed in one line. The EW, BW, SM and egg production efficiency between 169-280 days were included in the index as selection criteria. In this study, expected theoretical responses from the index were compared with actual responses obtained by the regression of average predicted breeding values by birth year multiplied by number of generation selected. Breeding values of five traits were predicted with multiple-trait BLUP under animal models using (co) variances estimated with REML. Directions of the actual responses for the traits except BW agreed well with those of expected theoretical responses in all lines. Disagreement found in BW was caused by the difference between assumed genetic correlations and REML ones. The amount of responses was less than theoretically expected and differed across lines reflecting the discrepancy between genetic parameters assumed and REML estimates in each line. The results indicate that more reliable genetic parameters for a line under question, the so-called REML estimates, should be used in order to attain pre-specified objectives.

  Japanese Society of Animal Science, Nov. 1996, Nihon Chikusan Gakkaiho, 67(11) (11), 941 - 948, English


• Determination of Optimum Mating Design With Constraints on Inbreeding Level via a Simple Genetic Algorithm

  OYAMA Kenji, MUKAI Fumio

  Application of a simple genetic algorithm (GA) was examined to determine the optimum mating designs with constraints on inbreeding level because selection based on predicted breeding values always involves a risk of rapid increase in inbreeding. The GAs search for the optimum solution imitating biological evolution through 'crossover of chromosomes', 'mutation of genes', and 'selections'. The operators used in GA were first evaluated without constraints on inbreeding level. Numbers of chromosomes and of crossovers had little effect on performance of GA. However as mutation rate decreased, performance of GA increased. When constraints were applied on inbreeding level, GA found solutions if appropriate penalties were given to those chromosomes, which resulted in unacceptable inbreeding levels. In this study it became clear that penalty was depended on solution spaces allowed for GA. Although moreresearch is required on determination of operators, GA showed satisfactory performances to search for the optimum mating designs.

  Japanese Society of Animal Science, Oct. 1996, Nihon Chikusan Gakkaiho, 67(10) (10), 835 - 843, English


• Genetic Relationships Among Traits Recorded at Registry Judgment, Reproductive Traits of Breeding Females and Carcass Traits of Fattening Animals in Japanese Black Cattle

  OYAMA Kenji, MUKAI Fumio, YOSHIMURA Toyonobu

  Abstract Genetic relationships among traits recorded on breeding females and carcass traits of fattening animals in Japanese Black cattle were estimated using REML procedure under multiple-trait animal models. Traits analyzed were five exterior judging traits, three body measurements at registry judgment, two reproductive traits (age at first calving, AFC; average calving interval, ACI) of breeding females and five carcass traits collected in Hiroshima prefecture. Heritabilities of exterior judging traits, body measurements and carcass traits were estimated at moderate to high, while those of reproductive traits were very low. Genetic correlations among exterior judging traits and body measurements were high when the ratio of body weight to withers height was not included in analytical model. Generally exterior judging traits, body measurements and carcass traits correlated favorably with AFC. However carcass beef marbling score correlated unfavorably with ACI. Because selection on carcass traits will be more intense, considerations will be necessary on ACI.

  Japanese Society of Animal Science, Jun. 1996, Nihon Chikusan Gakkaiho, 67(6) (6), 511 - 518, English


• Genetic relationships between performance test traits and field carcass traits in Japanese Black cattle

  F Mukai, K Oyama, S Kohno

  Genetic relationships between body measurements and growth traits at performance testing of bull calves and field carcass traits in Japanese Black cattle were estimated using a two-trait sire and maternal grandsire model with REML procedure. Covariances between performance test and carcass traits were obtained from across environment analyses. Performance test traits included wither height, chest girth, chest depth, thurl width, body weight, and daily gain. For field carcass traits carcass weight, longissimus muscle area, rib thickness, subcutaneous fat thickness, yield estimate, and beef marbling score were measured on 8329 steers and heifers. Heritabilities for performance test traits ranged from 0.13 to 0.36, and for carcass traits from 0.39 to 0.55. The basis of selection at performance testing, daily gain, did not correlate well with carcass beef marbling score, the breeding goal, indicating that genetically superior bull calves in marbling may be culled at the end of testing. Chest girth at the middle and the end of testing correlated well in the desired directions with all carcass traits. It is possible to improve total merit of the carcass by introducing chest girth into performance testing.

  ELSEVIER SCIENCE BV, Dec. 1995, LIVESTOCK PRODUCTION SCIENCE, 44(3) (3), 199 - 205, English

  [Refereed]

  Scientific journal


• Genetic Relationships between Live Animal Measurements at Performance Testing and Field Carcass Traits of Japanese Black Cattle

  OYAMA Kenji, MUKAI Fumio, HARADA Hiroshi

  Genetic relationships between ultrasonically measured live animal measurements and carcass traits in Japanese Black cattle were estimated using a two-trait sire and maternal grandsire model with REML procedure. Live animal measurements included longissimus muscle area (7-REAu), subcutaneous fat thickness (SFTu), intermuscular fat thickness (IMFTu), rib thickness (RTu) and beef marbling score (BMSu) at 7th rib, and longissimus muscle area at 13th rib (13-REAu) by ultrasound on 154 performance tested bulls. For carcass traits, carcass weight (CWc), longissimus muscle area (REAc), rib thickness (RTc), subcutaneous fat thickness (SFTc), yield estimate (YEc), and beef marbling score (BMSc) were measured on 4, 725 steers and heifers. Heritabilities for live animal measurements, at the end of performance testing, were estimated at 0.20, 0.58, and 0.15 for 7-REAu, SFTu, and BMSu, respectively. For carcass traits, heritabilities of 0.25 for CWc, 0.52 for REAc, 0.55 for SFTc, and 0.56 for BMSc were obtained. Carcass traits were highly heritable and had higher heritability estimates compared to those for ultrasonic measurements. Genetic correlations between ultrasonic longissimus muscle area and REAc ranged from 0.52 to 0.71. Ultrasonic subcutaneous fat thickness correlated negatively to SFTc and BMSu correlated positively to BMSc at the beginning of testing but negatively at the end of testing. Although ultrasonic live animal measurements concerning fat deposition did not correlate consistently to corresponding carcass traits, possibilities for improving REAc were indicated by the introduction of ultrasonic techniques into performance testing.

  Japanese Society of Animal Science, Jan. 1995, Nihon Chikusan Gakkaiho, 66(1) (1), 7 - 15, English

• Position tracking using low power radio for analyzing interaction among grazing cattle

  太田 能, 大川 剛直, 大山 憲二

  電子情報通信学会, 10 Jul. 2019, 電子情報通信学会技術研究報告 = IEICE technical report : 信学技報, 119(106) (106), 139 - 144, Japanese


• 省電力無線タグ受信電力に基づく時系列を考慮した深層学習による屋外位置推定

  山西 雄大, 治京 拓人, 高木 由美, 鎌田 十三郎, 太田 能, 西出 亮, 大山 憲二, 大川 剛直

  Mar. 2019, 電子情報通信学会総合大会予稿集, B-18-6, Japanese

  Summary national conference


• 省電力無線タグのモデルベース受信電力推定データを用いた深層学習に基づく屋外位置推定方式に関する一検討

  治京 拓人, 山西 雄大, 高木 由美, 太田 能, 大川 剛直, 西出 亮, 大山 憲二, 鎌田 十三郎

  Mar. 2019, 電子情報通信学会総合大会予稿集, B-18-5, Japanese

  Summary national conference


• 遺伝的多様性確保に向けた新たな改良指標の設定

  福島護之, 小浜菜美子, 秋山敬孝, 秋山敬孝, 吉田恵実, 坂瀬充洋, 加登岳史, 大山憲二

  2019, 肉用牛研究会報, (106) (106)


• 複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討

  山本雷斗, 川口芙岐, 秋山敬考, 吉田恵実, 小浜菜美子, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

  2019, 日本畜産学会大会講演要旨, 125th


• 兵庫県産黒毛和種の枝肉単価に対するモノ不飽和脂肪酸割合及び細かさ指数の影響

  小浜菜美子, 小浜菜美子, 秋山敬孝, 大山憲二

  2019, 日本畜産学会大会講演要旨, 126th


• 但馬牛の系統分類が長期的な遺伝的多様性に与える効果

  山田栞, 本多健, 小浜菜美子, 坂瀬充洋, 大山憲二

  2019, 日本畜産学会大会講演要旨, 126th


• 兵庫県黒毛和種集団におけるSLC27A6遺伝子多型のロース脂肪割合に対する効果の検討

  中島彩華, 栖原功太郎, 小林栄治, 福島護之, 吉田恵実, 秋山敬孝, 小浜菜美子, 大山憲二, 本多健, 万年英之, 笹崎晋史

  2018, 日本畜産学会大会講演要旨, 124th


• ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索

  川口芙岐, 木越洸利, 小浜菜美子, 秋山敬孝, 福島護之, 吉田恵実, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

  2018, 日本畜産学会大会講演要旨, 124th


• 肥育後期の黒毛和種における腰最長筋の脂肪酸組成の変化

  大山 憲二

  畜産技術協会, Feb. 2017, 畜産技術 = Livestock technology, (741) (741), 5 - 8, Japanese


• Identification of SNP associated with fertility trait using pool-based genome-wide association study in Japanese Black cattle.

  H. Ozaki, T. Tamura, K. Fukazawa, Y. Uemoto, M. Nishio, E. Kobayashi, T. Matsuhashi, S. Maruyama, T. Honda, K. Oyama, S. Sasazaki, H. Mannen

  AMER SOC ANIMAL SCIENCE, Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 21 - 21, English

  Summary international conference


• Identification of polymorphisms associated with oleic acid percentage by pool-based genome-wide association study in Japanese Black cattle.

  F. Kawaguchi, A. Nakajima, Y. Matsumoto, Y. Uemoto, M. Fukushima, E. Yoshida, E. Iwamoto, T. Akiyama, N. Kohama, E. Kobayashi, T. Honda, K. Oyama, H. Mannen, S. Sasazaki

  AMER SOC ANIMAL SCIENCE, Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 17 - 18, English

  Summary international conference


• Genome-wide association study identifies a QTL for fat percentage in ribeye area on BTA10 in Japanese Black cattle.

  A. Inoue, T. Nakajima, A. Nakajima, Y. Uemoto, M. Fukushima, E. Yoshida, E. Iwamoto, T. Akiyama, N. Kohama, E. Kobayashi, K. Oyama, T. Honda, H. Mannen, S. Sasazaki

  AMER SOC ANIMAL SCIENCE, Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 17 - 17, English

  Summary international conference


• 胆馬牛における胸最長筋,背半棘筋及び僧帽筋の画像解析形質に関する遺伝的パラメータの推定

  KOHAMA NAMIKO, SHOJI REIKO, AKIYAMA TOSHITAKA, SAKASE MITSUHIRO, OKA AKIO, FUKUSHIMA MORIYUKI, OYAMA KENJI

  28 Mar. 2015, 日本畜産学会大会講演要旨, 119th, 162, Japanese


• 兵庫県黒毛和種集団の遺伝的多様性に対する「系統らしさ」指標の有効性

  NAKATSUKA MACHI, HONDA TAKESHI, FUKUSHIMA MORIYUKI, OYAMA KENJI

  28 Mar. 2015, 日本畜産学会大会講演要旨, 119th, 163, Japanese


• 但馬系黒毛和種における在胎日数の年次推移とそれに影響を及ぼす要因の解明

  FUKUSHIMA MORIYUKI, YOSHIDA EMI, KOHAMA NAMIKO, AKIYAMA TAKAYUKI, SAKASE MITSUHIRO, OYAMA KENJI

  24 Jan. 2013, 肉用牛研究会報, 94(94) (94), 51 - 52, Japanese


• ウシFABP4遺伝子のプロモーター領域に対する多型探索

  松本大和, 大山憲二, 万年英之, 笹崎晋史

  日本動物遺伝育種学会, 01 Dec. 2012, 動物遺伝育種研究, 40(2) (2), 78 - 78, Japanese


• 岐阜県黒毛和種集団におけるUTS2R遺伝子内多型の脂肪酸組成に対する効果

  石井淳, 山地啓太, 阪本嵩幸, 秋山賢斗, 上本吉伸, 笹子奈々恵, 小林栄治, 小林直彦, 松橋珠子, 丸山新, 松本大和, 笹崎晋史, 大山憲二, 万年英之

  01 Dec. 2012, 動物遺伝育種研究, 40(2) (2), 73, Japanese


• 黒毛和種におけるLEP遺伝子内多型の脂肪酸組成に対する効果

  大倉一輝, 松本大和, 笹崎晋史, 大山憲二, 万年英之

  01 Dec. 2012, 動物遺伝育種研究, 40(2) (2), 85, Japanese


• 黒毛和種におけるFASN遺伝子内多型の脂肪酸組成に対する効果

  阪本嵩幸, 石井淳, 山地啓太, 早川きり, 上本吉伸, 笹子奈々恵, 高須賀晶子, 小林栄治, 小林直彦, 松橋珠子, 丸山新, 平野貴, 杉本喜憲, 松本大和, 笹崎晋史, 大山憲二, 万年英之

  01 Dec. 2012, 動物遺伝育種研究, 40(2) (2), 72, Japanese


• 大容量データによる黒毛和種雌牛の繁殖形質についての遺伝率および反復率の推定

  松田 洋和, 谷口 幸雄, 勝田 智博, 大山 憲二, 祝前 博明

  28 Jan. 2012, 肉用牛研究会報, 92, 64 - 64, Japanese


• ホルスタイン集団におけるsliding window approachを利用した乳形質関連遺伝子座の探索

  山地啓太, 石井淳, 細川大介, 松本大和, 笹崎晋史, 大山憲二, 万年英之

  01 Dec. 2011, 動物遺伝育種研究, 39(2) (2), 133, Japanese


• Genetic variability of Wagyu cattle estimated by statistical approaches

  Kenji Oyama

  The genetic evaluation of economically important traits utilizes estimates of genetic variability, which are represented by heritability. This review summarizes the published heritabilities of traits estimated in Wagyu cattle. Two different mean heritabilities, unweighted and weighted by standard errors, were calculated. In Japanese Black cattle, the average unweighted and weighted direct heritabilities of birth weight were 0.35 and 0.28, respectively, whereas the respective maternal heritabilities were 0.17 and 0.07. The mean unweighted heritability of calf market weight was estimated to be 0.30 in Japanese Black cattle. The mean unweighted heritability of daily gain during performance testing was 0.29 in Japanese Black and 0.40 in Japanese Shorthorn cattle. In Japanese Black cattle, the unweighted mean heritability was 0.48 for carcass weight, 0.46 for rib-eye area, 0.38 for rib thickness, 0.39 for subcutaneous fat thickness, and 0.55 for marbling. The mean weighted heritability of the calving interval was low, and estimated to be 0.05. In general, the heritabilities estimated in Wagyu cattle were similar to those estimated in other beef breeds.

  WILEY-BLACKWELL, 2011, ANIMAL SCIENCE JOURNAL, 82(3) (3), 367 - 373, English

  [Refereed]

  Book review


• 鳥取和牛肉の脂肪酸組成割合に関する遺伝的パラメータの推定

  野儀 卓哉, 大山 憲二

  鳥取県農林水産部農林総合研究所畜産試験場, Dec. 2008, 鳥取県農林水産部農林総合研究所畜産試験場研究報告, (36) (36), 14 - 21, Japanese


• 科学を用いた美味しさの追及「神戸大学ビーフ」(大学発!美味しいバイオ)

  大山 憲二, 万年 英之

  日本生物工学会, 2008, 生物工学会誌 : seibutsu-kogaku kaishi, 86(8) (8), 410 - 411, Japanese


• 種雄牛造成や交配種雄牛助言サービスソフト「MSAS」の利用が但馬牛集団に及ぼす影響

  福島 護之, 坂瀬 充洋, 野田 昌伸, 武田 和士, 田中 幹雄, 上野 悟, 大山 憲二

  20 Dec. 2005, 肉用牛研究会報, 80, 7 - 9, Japanese


• Estimation of genetic parameters for growth and feed utilization traits in Japanese Black cattle

  Masayuki Shojo, Jiang Yong, Katsuhito Anada, Kenji Oyama, Fumio Mukai

  Performance test results of 3250 sire candidates were used to estimate the genetic parameters of growth and feed utilization traits in Japanese Black cattle. Growth traits analyzed were six body measurements at the end of the performance test and daily gain (DG) during the test. Feed utilization traits were intakes and conversions of concentrate, roughage, digestible crude protein and total digestible nutrient (TDN). Genetic (co)variance components were estimated by the restricted maximum likelihood procedure using an expectation maximization algorithm under the two-trait animal model. Heritabilities for growth traits ranged from 0.40 to 0.70 and for feed utilization traits from 0.21 to 0.74. Genetic correlations of DG were positive with feed intake (0.15-0.77) and negative with feed conversions (-0.63 to -0.30). These relationships indicate that the selection based on DG improves feed efficiency but it simultaneously increases feed intake. Feed conversions showed genetic correlations ranging from -0.09 to 0.03 with total available energy consumption, TDN intake. Thus the results suggested that feed conversions were not efficient selection criteria to decrease TDN intake and to improve comprehensive feed utilization ability.

  Apr. 2005, Animal Science Journal, 76(2) (2), 115 - 119, English

  Others


• 交配種雄牛助言サービスソフト(MSAS)の繁殖現場での利用効果と問題点

  福島 護之, 坂瀬 充洋, 野田 昌伸, 山下 寿也, 太田 泰浩, 林 孝志, 大山 憲二

  22 Jan. 2003, 肉用牛研究会報, 74, 7 - 9, Japanese


• 但馬牛集団の血縁係数による系統分類の試み

  福島 護之, 大山 憲二, 坂瀬 充洋, 野田 昌伸, 武田 和士, 有吉 哲志, 向井 文雄

  血縁係数を利用したクラスター分析により但馬牛集団の系統分類を試み、現存集団の遺伝的構成を把握し、系統の再構築の可能性を検討した。とくに、今回の分析で規模が小さな集団の増頭を目的として、仮想集団を作出した場合の系統分類についても検討した。1.供用中集団のデンドログラムを血縁係数0.10で切断し、7グループに分類した。2.各グループ内及びグループ間の平均血縁係数は、グループ1、2、3と5では全て0.3以上であった。グループ4も概ね0.3で上記グループとの血縁が高かった。グループ6と7が、他グループと異なりグループ間の血縁係数が低かった。3.各グループの育種価評価値の平均値を比較するとグループ毎に産肉あるいは種牛能力の特徴が明らかになった。4.600頭の仮想雌牛群と待機雄牛の後代を加え系統分類を実施した。その結果、中土井系繁殖雌牛に城崎系種雄牛を交配するシミュレーション集団1では、雌雄が逆の系統に属する交配集団2に比較して高率(1.7%vs16.0%)に城崎系であるグループが作出された。集団1では、種雄牛による差が大きく、奥秀、城富、城和、勘高や大勘で城崎系産子を生産する割合が高かったが、光城波は亜系の新たなグループを形成した。集団2では、城崎系を生産する母牛が6頭程度に限られ、中土井系の種雄牛を交配することで城崎系の産子を生産する可能性は低かった。

  兵庫県立農林水産技術総合センター, 2003, 兵庫県立農林水産技術総合センター研究報告〔畜産編〕, 39,pp.16-21(39) (39), 16 - 21, Japanese

  Others


• 予測育種価に基づく黒毛和種の遺伝的改良

  大山 憲二

  畜産技術協会, Aug. 2001, 畜産技術, (555) (555), 17 - 20, Japanese


• 但馬牛における生後270日齢までの平均発育曲線の推定

  野田 昌伸, 木伏 雅彦, 福島 護之, 大山 憲二, 高橋 毅

  25 Dec. 2000, 肉用牛研究会報, 70, 27 - 28, Japanese


• Property of Difference between Two Predicted Breeding Values from BLUP Methodology

  OYAMA Kenji, OTANI Shinji, MUKAI Fumio

  日本畜産学会, 25 Sep. 1999, Animal science journal, 70(5) (5), 268 - 272, English


• Property of Difference between Two Predicted Breeding Values from BLUP Methodology

  OYAMA Kenji, OTANI Shinji, MUKAI Fumio

  25 Sep. 1999, 日本畜産學會報 = The Japanese journal of zootechnical science, 70(5) (5), 268 - 272, English


• Estimation of Genetic Parameters by Restricted Maximum Likelihood under Multi-trait Animal Models in Selected Layer Lines

  DANBARO Gariba, OYAMA Kenji, MUKAI Fumio, TSUJI Soichi, MIYATA Toru

  日本万国家禽学会, 25 May 1996, Japanese poultry science, 33(3) (3), 185 - 192, English


• Genetic Trends from a Selection Experiment in Broiler Breeder Lines Using Best Linear Unbiased Prediction Methodology

  DANBARO Gariba, OYAMA Kenji, MUKAI Fumio, TSUJI Soichi, TATEISHI Tomonori, MAE Michio

  日本万国家禽学会, 25 Nov. 1995, Japanese poultry science, 32(6) (6), 379 - 386, English


• Heritabilities and Genetic Correlations from a Selection Experiment in Broiler Breeders using Restricted Maximum Likelihood

  DANBARO Gariba, OYAMA Kenji, MUKAI Fumio, TSUJI Soichi, TATEISHI Tomonori, MAE Michio

  日本万国家禽学会, 25 Jul. 1995, Japanese poultry science, 32(4) (4), 257 - 266, English


• 肥育農家を変量効果としたときの枝肉形質の遺伝的評価への影響

  吉上 渉, 向井 文雄, 大山 憲二

  広島県立畜産試験場, Mar. 1995, 広島県立畜産試験場研究報告, (10) (10), p55 - 62, Japanese

• アグリバイオ 特集「和牛の育種改良」

  大山憲二

  Contributor, 北隆館, Jan. 2020


• 兵庫の和牛 但馬牛－育種価評価による改良の推進

  OYAMA KENJI

  Others, 丸善プラネット, Mar. 2018, Japanese

  Scholarly book


• 動物遺伝育種学－選抜と選抜育種

  OYAMA KENJI

  Others, 朝倉書店, Mar. 2017, Japanese

  Textbook


• エシカルな農業－未来の但馬牛のために今すべきこと

  OYAMA KENJI

  Others, 誠文堂新光社, Oct. 2016, Japanese

  General book


• 最新畜産ハンドブック

  OYAMA KENJI

  Others, 講談社, Jul. 2014, Japanese, ISBN: 9784061537392

  Others


• 生物統計学

  OYAMA KENJI

  Joint work, 化学同人, Mar. 2011, Japanese

  Textbook

• 黒毛和種肥育牛における脂肪壊死の発症予測性について

  小西幹生, 吉川未来, 本多健, 大山憲二

  第61回肉用牛研究会兵庫大会, Oct. 2024

  Oral presentation


• 「神戸大学ビーフ」の生産とブランド化

  大山 憲二

  近畿大学×神戸大学SDGsフォーラム, Jul. 2023

  [Invited]

  Public discourse


• 未来の但馬牛が見えますか？

  大山 憲二

  兵庫県朝来家畜保健衛生所令和4年度和牛講習会, Feb. 2023

  [Invited]

  Public discourse


• 和牛育種組合連合会第60回通常総会

  [Invited]


• 湯村温泉但馬ビーフ流通振興協議会総会

  [Invited]


• 平成3年度日本獣医師会獣医学術学会年次大会シンポジウム

  [Invited]


• Genetic improvement of Japanese Black / Overview of Japanese Breeding Programs

  Oyama K

  WagyuEdge Conference, Apr. 2021

  [Invited]


• 但馬牛の系統分類が長期的な遺伝的多様性に与える効果

  山田 栞, 本多 健, 小浜菜美子, 坂瀬充洋, 大山憲二

  日本畜産学会第126回大会, Sep. 2019

  Oral presentation


• 兵庫県産黒毛和種の枝肉単価に対するモノ不飽和脂肪酸割合及び細かさ指数の影響

  小浜菜美子, 秋山敬孝, 大山憲二

  日本畜産学会第126回大会, Sep. 2019

  Oral presentation


• Validation of candidate SNPs for fat percentage in bovine rib-eye area using Integrated Fluidic Circuit

  Yamamoto R, Toyomoto S, Kohama N, Akiyama T, Yoshida E, Kobayashi E, Oyama K, Mannen H, Sasazaki S

  34th International Society for Animal Genetics Conference, Jul. 2019, English, Lleida, Spain, International conference

  Poster presentation


• Effect of the PLAG1 gene polymorphism on carcass weight and oleic acid percentage in Japanese Black cattle populations

  Kawaguchi F, Kigoshi H, Yasuzumi R, Oyama K, Mannen H, Sasazaki S

  34th International Society for Animal Genetics Conference, Jul. 2019, English, Lleida, Spain, International conference

  Poster presentation


• 複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討

  山本雷斗, 川口芙岐, 秋山 敬孝, 吉田 恵実, 小浜 菜美子, 小林 栄治, 大山 憲二, 万年 英之, 笹崎 晋史

  第125回日本畜産学会大会, Mar. 2019, Japanese, 日本畜産学会, 相模原, Domestic conference

  Oral presentation


• 省電力無線タグ受信電力に基づく時系列を考慮した深層学習による屋外位置推定

  YAMANISHI Takahiro, JIKYO Takuto, TAKAKI Yumi, KAMADA Tomio, OHTA Chikara, NISHIDE Ryo, OYAMA Kenji, OHKAWA Takenao

  電子情報通信学会総合大会, Mar. 2019, Japanese, 電子情報通信学会, 新宿区, Domestic conference

  Oral presentation


• 省電力無線タグのモデルベース受信電力推定データを用いた深層学習に基づく屋外位置推定方式に関する一検討

  JIKYO Takuto, YAMANISHI Takahiro, TAKAKI Yumi, OHTA Chikara, OHKAWA Takenao, NISHIDE Ryo, OYAMA Kenji, KAMADA Tomio

  電子情報通信学会総合大会, Mar. 2019, Japanese, 電子情報通信学会, 新宿区, Domestic conference

  Oral presentation


• 遺伝的多様性確保に向けた新たな改良指標の設定

  福島護之, 小浜菜美子, 秋山敬孝, 吉田恵実, 坂瀬充洋, 加登岳史, OYAMA KENJI

  第56回肉用牛研究会愛媛大会, Nov. 2018, Japanese, Domestic conference

  Oral presentation


• 兵庫県黒毛和種集団における全ゲノムリシーケンスデータを用いた枝肉形質に対する候補変異の抽出

  木越洸利, 川口芙岐, 大山憲二, 万年英之, 笹崎晋史

  第18回日本動物遺伝育種学会大会, Sep. 2018, Japanese, 京都, Domestic conference

  Oral presentation


• 黒毛和種で発生する白斑と枝肉形質との関連性の調査

  小山秀美, 有木 啓, 今村清人, 坂元信一, 大里和弘, 西 和隆, 河邊弘太郎, 岡本 新, 本多健, OYAMA KENJI, 下桐 猛

  日本動物遺伝育種学会第18回大会, Sep. 2018, Japanese, Domestic conference

  Poster presentation


• ゲノムリシーケンスデータを用いたウシ10番染色体におけるロース脂肪割合の原因変異の探索

  安栖梨理, 井上麻美, 小林 栄治, 福島 護之, 小浜 菜美子, 大山 憲二, 本多 健, 万年 英之, 笹崎 晋史

  第18回日本動物遺伝育種学会大会, Sep. 2018, Japanese, 京都, Domestic conference

  Oral presentation


• IoTを活用した放牧牛のインタラクション分析に基づく飼養管理技術の開発

  NISHIDE Ryo, OHKAWA Takenao, OYAMA Kenji, OHTA Chikara, TAKAKI Yumi

  平成30年度 第68回関西畜産学会大会, Sep. 2018, Japanese, 関西畜産学会, Domestic conference

  Oral presentation


• Effect of SLC27A6 gene K81M polymorphism on fat percentage in rib-eye area in Japanese Black cattle

  Toyomoto S, Kohama N, Akiyama T, Fukushima M, Yoshida E, Kobayashi E, Oyama K, Mannen H, Sasazaki S

  PAG Asia 2018, May 2018, English, Scherago International, Seoul, Korea, International conference

  Poster presentation


• Detection of candidate polymorphisms in the QTL for oleic acid percentage on BTA9 based on whole-genome resequencing data

  Kawaguchi F, Kigoshi H, Kohama N, Akiyama T, Fukushima M, Yoshida E, Kobayashi E, Oyama K, Mannen H, Sasazaki S

  PAG Asia 2018, May 2018, English, Scherago International, Seoul, Korea, International conference

  Poster presentation


• 兵庫県黒毛和種集団におけるSLC27A6遺伝子多型のロース脂肪割合に対する効果の検討

  中島彩華, 栖原功太郎, 小林 栄治, 福島 護之, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 大山 憲二, 本多 健, 万年 英之, 笹崎 晋史

  第124回日本畜産学会大会, Mar. 2018, Japanese, 日本畜産学会, 東京, Domestic conference

  Oral presentation


• ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索

  川口芙岐, 木越洸利, 小浜菜美子, 秋山敬孝, 福島護之, 吉田恵実, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

  第124回日本畜産学会大会, Mar. 2018, Japanese, 日本畜産学会, 東京, Domestic conference

  Oral presentation


• 兵庫県黒毛和種におけるゲノムワイド関連解析により同定されたVNN1遺伝子T66M多型のオレイン酸含有率に対する効果

  木越洸利, 川口芙岐, 上本吉伸, 福島護之, 吉田恵実, 岩本英治, 秋山敬孝, 小浜菜美子, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

  第18回日本動物遺伝育種学会大会, Nov. 2017, Japanese, 動物遺伝育種学会, 厚木, Domestic conference

  Oral presentation


• 複数の黒毛和種集団を用いたPLAG1遺伝子多型のオレイン酸含有率に対する効果の検討

  川口芙岐, 木越洸利, 安栖梨理, 小浜菜美子, 秋山敬孝, 福島護之, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

  第18回日本動物遺伝育種学会大会, Nov. 2017, Japanese, 動物遺伝育種学会, 厚木, Domestic conference

  Oral presentation


• ゲノムリシーケンスデータを利用したウシ7番染色体におけるロース脂肪割合に関わる遺伝子変異の探索

  中島彩華, 中嶋翼, 上本吉伸, 小林 栄治, 福島 護之, 岩本 英治, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 大山 憲二, 本多 健, 万年 英之, 笹崎 晋史

  第67回関西畜産学会大会, Sep. 2017, Japanese, 関西畜産学会, 大阪, Domestic conference

  Oral presentation


• 無線タグによる和牛の個体識別・トラッキングに関する取り組み

  OHTA Chikara, OHKAWA Takenao, OYAMA Kenji

  電子情報通信学会コミュニケーションクォリティ研究会研究会, Jul. 2017, Japanese, 電子情報通信学会, 神戸市, Domestic conference

  Invited oral presentation


• PLAG1 polymorphism (ss319607405) is associated with oleic acid percentage in Japanese Black cattle

  Kawaguchi F, kigoshi H, Nakajima A, Matsumoto Y, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Honda T, Oyama K, Mannen H, Sasazaki S

  36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

  Poster presentation


• Genome-wide association study revealed a candidate region for beef marbling on BTA7 in Japanese Black cattle

  Sasazaki S, Nakajima T, Nakajima A, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Oyama K, Mannen H

  36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

  Poster presentation


• Effects of DNA markers associated with carcass traits and fatty acid composition on fertility traits in Japanese Black cows

  Tsuchimura M, Fukazawa K, Matsuhashi T, Maruyama S, Oyama K, Sasazaki S, Mannen H

  36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

  Poster presentation


• Association of VNN1 gene polymorphism with fatty acid composition in Japanese Black cattle

  Kigoshi H, Matsumoto Y, Kawaguchi F, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Oyama K, Honda T, Mannen H, Sasazaki S

  36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

  Poster presentation


• 黒毛和種の損徴に関する遺伝育種学的研究

  小山 秀美, 今村 清人, 坂元 信一, 西 和隆, 河邊 弘太郎, 岡本 新, HONDA TAKESHI, OYAMA KENJI, 下桐 猛

  日本畜産学会第122回大会, Mar. 2017, Japanese, 神戸大学, Domestic conference

  Oral presentation


• 鹿児島県産黒毛和種で発生する白斑に関する遺伝育種学的研究

  小山 秀美, 今村 清人, 坂元 信一, 西 和隆, 河邊 弘太郎, 岡本 新, HONDA TAKESHI, OYAMA KENJI, 下桐 猛

  日本動物遺伝育種学会第17回大会, Nov. 2016, Japanese, Domestic conference

  Poster presentation


• 黒毛和種繁殖雌牛集団におけるレプチン遺伝子内多型と繁殖形質との関連

  川口芙岐, 深澤謙治, 小浜菜美子, 秋山敬孝, 福島護之, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

  第17回日本動物遺伝育種学会大会, Nov. 2016, Japanese, 日本動物遺伝育種学会, 名古屋, Domestic conference

  Oral presentation


• 鹿児島県産黒毛和種における死産と母牛の審査時形質との関連性

  小山 秀美, 西 和隆, 今村 清人, 坂元 信一, 河邊 弘太郎, 岡本 新, HONDA TAKESHI, OYAMA KENJI, 下桐 猛

  日本暖地畜産学会第9回山口大会, Oct. 2016, Japanese, Domestic conference

  Oral presentation


• ステレオカメラを用いた肥育牛の体重予測

  山下 歩, OYAMA KENJI, OHKAWA TAKENAO

  平成28年 電気学会 電子・情報・システム部門大会, Aug. 2016, Japanese, Domestic conference

  Oral presentation


• Estimation of genetic parameters for stillbirth of Japanese Black cattle in Kagoshima

  Oyama H, Nishi K, Imamura K, Sakamoto S, Kawabe K, Okamoto S, HONDA TAKESHI, OYAMA KENJI, Shimogiri T

  Proceedings of 17th AAAP Animal Science Congress, Aug. 2016, English, International conference

  Poster presentation


• Identification of SNP associated with fertility trait using pool-based genome-wide association study in Japanese Black cattle

  Ozaki H, Tamura T, Fukazawa K, Uemoto Y, Nishio M, Kobayashi E, Matsuhashi T, Maruyama S, Honda T, Oyama K, Sasazaki S, Mannen H

  35th International Society for Animal Genetics Conference, Jul. 2016, English, International Society for Animal Genetics, Slat Lake City, USA, International conference

  Poster presentation


• Identification of polymorphisms associated with oleic acid percentage by pool-based genome wide association study (GWAS) in Japanese Black

  Kawaguchi F, Nakajima A, Matsumoto Y, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Honda T, Oyama K, Mannen H, Sasazaki S

  35th International Society for Animal Genetics Conference, Jul. 2016, English, International Society for Animal Genetics, Slat Lake City, USA, International conference

  Poster presentation


• Genome-wide association study identifies a QTL for fat percentage in rib-eye area on BTA10 in Japanese Black cattle

  Inoue A, Nakajima T, Nakajima A, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Oyama K, Mannen H, Sasazaki S

  35th International Society for Animal Genetics Conference, Jul. 2016, English, International Society for Animal Genetics, Slat Lake City, USA, International conference

  Poster presentation


• ウシ10番染色体におけるロース脂肪割合に影響する遺伝子の探索

  井上麻美, 中島彩華, 中嶋翼, 上本吉伸, 小林 栄治, 福島 護之, 岩本 英治, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 大山 憲二, 本多 健, 万年英之, 笹崎晋史

  第121回日本畜産学会大会, Mar. 2016, Japanese, 日本畜産学会, 東京, Domestic conference

  Poster presentation


• 兵庫県黒毛和種集団の遺伝的多様性に対する「系統らしさ」指標の長期的な有効性

  NAKATSUKA MACHI, HONDA TAKESHI, FUKUSHIMA MORIYUKI, OYAMA KENJI

  日本動物遺伝育種学会第16回大会, Nov. 2015, Japanese, 神戸, Domestic conference

  Poster presentation


• 兵庫県黒毛和種集団におけるオレイン酸含有率に対するゲノムワイド関連解析

  松本祐汰, 中島彩華, 上本 吉伸, 小林栄治, 福島 護之, 岩本 英治, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 笹崎晋史, 本多健, 大山憲二, 万年英之

  第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference

  Poster presentation


• 黒毛和種における産肉形質関連マーカーの繁殖形質に対する効果の推定

  深澤謙治, 笹崎晋史, 大山憲二, 本多健, 小林栄治, 福島 護之, 秋山 敬孝, 小浜 菜美子, 万年英之

  第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference

  Poster presentation


• 岐阜県黒毛和種の繁殖形質を対象としたゲノムワイド関連解析

  田村朋子, 深澤謙治, 尾崎弘樹, 上本吉伸, 西尾元秀, 小林栄治, 松橋珠子, 丸山新, 本多健, 笹崎晋史, 大山憲二, 万年英之

  第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference

  Poster presentation


• GWASを用いた黒毛和種集団におけるロース脂肪割合に関わる遺伝子座の探索

  中嶋翼, 中島彩華, 上本吉伸, 小林 栄治, 福島 護之, 岩本 英治, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 大山憲二, 本多健, 万年英之, 笹崎晋史

  第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference

  Poster presentation


• 放牧場の俯瞰動画データを用いた繁殖牛のインタラクション検出

  細見 洋司, 大川 剛直, 大山 憲二, 本多 健

  第62回情報システム研究会, May 2015, Japanese, 神戸大学, Domestic conference

  Oral presentation


• GPSを利用した放牧牛のインタラクション情報の取得とその分析

  松本 健吾, 大川 剛直, 大山 憲二, 本多 健

  第62回情報システム研究会, May 2015, Japanese, 神戸大学, Domestic conference

  Oral presentation


• 兵庫県黒毛和種集団の遺伝的多様性に対する「系統らしさ」指標の有効性

  NAKATSUKA MACHI, HONDA TAKESHI, FUKUSHIMA MORIYUKI, OYAMA KENJI

  日本畜産学会, Mar. 2015, Japanese, 宇都宮大学, Domestic conference

  Oral presentation


• 但馬牛における胸最長筋，背半棘筋及び僧帽筋の画像解析形質に関する遺伝的パラメータの推定

  小浜 菜美子, 小路 怜子, 秋山 敬孝, 坂瀬 充洋, 岡 章生, 福島 護之, OYAMA KENJI

  日本畜産学会第119回大会, Mar. 2015, Japanese, 宇都宮市, Domestic conference

  Oral presentation


• 黒毛和種肥育牛1047頭を用いたオレイン酸割合ゲノム育種価評価の試み

  田淵 一郎, 北村 夕貴, 野儀 卓也, OYAMA KENJI, 杉本 喜憲, 渡邊 敏夫

  日本畜産学会第119回大会, Mar. 2015, Japanese, 宇都宮市, Domestic conference

  Oral presentation


• 黒毛和種集団におけるLeptin遺伝子翻訳領域内多型の脂肪酸組成に対する効果

  川口芙岐, 大倉一輝, 大山憲二, 万年英之, 笹崎晋史

  第118回日本畜産学会大会, Mar. 2015, Japanese, 日本畜産学会, 宇都宮, Domestic conference

  Oral presentation


• 黒毛和種における内臓疾病に関連した経済的損失

  INOUE KEIICHI, HONDA TAKESHI, OYAMA KENJI

  日本畜産学会, Mar. 2015, Japanese, 宇都宮大学, Domestic conference

  Oral presentation


• 見島ウシの集団構造に関する基礎的研究

  HIRAMOTO AGEHA, HONDA TAKESHI, 稲吉 洋裕, OYAMA KENJI

  日本畜産学会, Mar. 2015, Japanese, 宇都宮大学, Domestic conference

  Oral presentation


• 肥育後期の黒毛和種における 不飽和脂肪酸の体内変化について

  OYAMA KENJI

  平成26年度現場後代検定合同調査会シンポジウム, Feb. 2015, Japanese, 神戸市, Domestic conference

  [Invited]

  Nominated symposium


• ホルスタイン種におけるFASN-g.841 及びg.16024 多型の乳形質に対する効果

  早川きり, 阿部剛, 長谷部浩行, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

  第15回日本動物遺伝育種学会大会, Nov. 2014, Japanese, 日本動物遺伝育種学会, 和光, Domestic conference

  Poster presentation


• 牛肉ブランドの現状と今後の方向性

  OYAMA KENJI

  2014年度農業施設学会大会シンポジウム, Aug. 2014, Japanese, 神戸市, Domestic conference

  [Invited]

  Nominated symposium


• LPL gene polymorphisms are associated with fatty acid composition in Japanese Black

  Koga S, Oyama K, Sasazaki S, Mannen H

  34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conference

  Oral presentation


• Genome wide association study revealed candidate regions for calving interval in Japanese black cattle

  Kawasaki Y, Fukazawa K, Fujii T, Uemoto Y, Fukushima M, Akiyama T, Kohama N, Kobayashi E, Oyama K, Sasazaki S, Mannen H

  34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conference

  Oral presentation


• A genome wide association study for beef marbling and fatty acid composition in Japanese Black cattle using pooled DNA

  Nakajima A, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Oyama K, Sasazaki S, Mannen H

  34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conference

  Oral presentation


• 鹿児島県産黒毛和種の枝肉における瑕疵間の遺伝相関の推定

  西 和隆, 下桐 猛, 草野 昭徳, 坂元 信一, 城元 清巳, 河邊 弘太郎, 岡本 新, 本多 健, 大山 憲二

  日本畜産学会第118回大会, Mar. 2014, Japanese, つくば市, Domestic conference

  Oral presentation


• 黒毛和種肥育牛を訓練集団としたオレイン酸割合ゲノム育種価評価の試み

  田淵 一郎, 野儀 卓也, OYAMA KENJI, 杉本 喜憲, 渡邊 敏夫

  日本畜産学会第118回大会, Mar. 2014, Japanese, つくば市, Domestic conference

  Poster presentation


• Searching for causal structures among internal diseases and carcass traits in Japanese Black cattle

  井上 慶一, Valente Brouno D, 本多 健, OYAMA KENJI, Rosa Guilherme J

  日本畜産学会第118回大会, Mar. 2014, English, つくば市, Domestic conference

  Poster presentation


• 黒毛和種におけるLEP遺伝子プロモーター領域に対する多型探索

  大倉 一輝, 笹崎 晋史, 大山 憲二, 万年 英之

  第14回日本動物遺伝育種学会大会, Oct. 2013, Japanese, 日本動物遺伝育種学会, 東京, Domestic conference

  Poster presentation


• ウシ脂肪酸組成に対するFASN 遺伝子内多型の効果

  早川 きり, 阪本 嵩幸, 石井 淳, 山地 啓太, 上本 吉伸, 笹子 奈々恵, 高須賀 晶子, 小林 栄治, 小林 直彦, 松橋 珠子, 丸山 新, 野偽 卓哉, 田淵 一郎, 平野 貴, 松本大和, 笹崎 晋史, 大山 憲二, 万年 英之

  第116回日本畜産学会大会, Mar. 2013, Japanese, 日本畜産学会, 広島, Domestic conference

  Oral presentation


• 兵庫系黒毛和種における育種集団毎の遺伝的多様性評価

  福島 護之, 本多 健, 小路 怜子, 小浜 菜美子, 秋山 敬孝, 坂瀬 充洋, 岡 章生, 大山 憲二

  日本畜産学会第117回大会, 2013, Japanese, Domestic conference

  Oral presentation


• 但馬牛における枝肉画像解析形質の遺伝的パラメータの推定

  小浜 菜美子, 小路 怜子, 秋山 敬孝, 坂瀬 充洋, 岡 章生, 福島 護之, 大山 憲二

  日本畜産学会第117回大会, 2013, Japanese, Domestic conference

  Oral presentation


• 鹿児島県産黒毛和種を用いた瑕疵と枝肉6形質の遺伝相関及び表型相関

  西 和隆, 下桐 猛, 草野 昭徳, 坂元 信一, 城元 清巳, 河邊 弘太郎, 岡本 新, 本多 健, 大山 憲二

  2013年度日本暖地畜産学会, 2013, Japanese, Domestic conference

  Oral presentation


• 鹿児島県産黒毛和種の枝肉で発生する瑕疵に関する遺伝的パラメータの推定

  西 和隆, 下桐 猛, 草野 昭徳, 坂元 信一, 城元 清巳, 河邊 弘太郎, 岡本 新, 本多 健, 大山 憲二

  日本畜産学会第117回大会, 2013, Japanese, Domestic conference

  Oral presentation


• 兵庫県黒毛和種集団における系統内交配による遺伝的分化の可能性

  木綿 萌, 本多 健, 福島 護之, 大山 憲二

  第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 日本動物遺伝育種学会, 仙台, Domestic conference

  Poster presentation


• 黒毛和種におけるLEP 遺伝子内多型の脂肪酸組成に対する効果

  大倉 一輝, 松本 大和, 笹崎 晋史, 大山 憲二, 万年 英之

  第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 日本動物遺伝育種学会, 仙台, Domestic conference

  Poster presentation


• 黒毛和種におけるFASN 遺伝子内多型の脂肪酸組成に対する効果

  阪本 嵩幸, 石井 淳, 山地 啓太, 早川 きり, 上本 吉伸, 笹子 奈々恵, 高須賀 晶子, 小林 栄治, 小林 直彦, 松橋 珠子, 丸山 新, 平野 貴, 杉本 喜憲, 松本 大和, 笹崎 晋史, 大山 憲二, 万年 英之

  第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 日本動物遺伝育種学会, 仙台, Domestic conference

  Poster presentation


• 岐阜県黒毛和種集団におけるUTS2R 遺伝子内多型の脂肪酸組成に対する効果

  石井 淳, 山地 啓太, 阪本 嵩幸, 秋山 賢斗, 上本 吉伸, 笹子 奈々恵, 小林 栄治, 小林 直彦, 松橋 珠子, 丸山 新, 松本 大和, 笹崎 晋史, 大山 憲二, 万年 英之

  第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 日本動物遺伝育種学会, 仙台, Domestic conference

  Poster presentation


• ウシFABP4 遺伝子のプロモーター領域に対する多型探索

  松本 大和, OYAMA KENJI, MANNEN HIDEYUKI, SASAZAKI SHINJI

  第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 仙台, Domestic conference

  Poster presentation


• UTS2R gene polymorphisms are associated with fatty acid composition in Japanese beef cattle

  Sasazaki Shinji, Akiyama Kento, Narukami Takahiro, Matsumoto Hirokazu, Oyama Kenji, Mannen Hideyuki

  33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

  Poster presentation


• The SNPs in the ACACA gene effective on fatty acid composition in Holstein milk

  Sakamoto Takayuki, Matsumoto Hirokazu, Sasaki Kenta, Bessho Takuya, Kobayashi Eiji, Abe Tsuyoshi, Sasazaki Shinji, Oyama Kenji, Mannen Hideyuki

  33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

  Poster presentation


• The SNP in the promoter region of the bovine ELOVL5 gene is effective on subcutaneous fat thickness

  MATSUMOTO Hirokazu, SHIMIZU Yumiko, TANAKA Atsuko, NOGI Takuya, TABUCHI Ichiro, Oyama Kenji, Mannen Hideyuki, Sasazaki Shinji

  33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

  Poster presentation


• Identification of quantitative trait loci affecting economic traits based on divergently selected regions between Japanese Black and Holstein cattle

  YAMAJI Keita, HOSOKAWA Daisuke, ISHII Atsushi, Sasazaki Shinji, Oyama Kenji, Mannen Hideyuki

  33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

  Poster presentation


• 和牛の改良と但馬牛

  大山 憲二

  第2回但馬牛改良セミナー, 2012, Japanese, Domestic conference

  Public discourse


• 但馬系黒毛和種における在胎日数の年次推移とそれに影響を及ぼす要因の解明

  福島 護之, 吉田 恵実, 小浜 菜美子, 秋山 敬孝, 坂瀬 充洋, 大山 憲二

  第50回肉用牛研究会, 2012, Japanese, Domestic conference

  Oral presentation


• 黒毛和種の肥育後期における胸最長筋および腰最長筋の脂肪酸組成の経時的変化

  小栗 佑介, 柳原 佳代子, 石田 孝史, 本多 健, 万年 英之, 小林 郁雄, 大山憲二

  第62回関西畜産学会大会, 2012, Japanese, Domestic conference

  Oral presentation


• プロローグ ～方舟に乗せるべき遺伝資源～

  大山 憲二

  神戸大学農学研究科公開講座, 2012, Japanese, Domestic conference

  Public discourse


• 黒毛和種およびホルスタインにおけるUTS2R遺伝子多型の脂肪酸組成に対する効果

  秋山 賢斗, 鳴神 貴太, 笹崎 晋史, 大山 憲二, 万年 英之

  第12回日本動物遺伝育種学会大会, Nov. 2011, Japanese, 日本動物遺伝育種学会, 広島, Domestic conference

  Poster presentation


• ホルスタイン集団におけるsliding window approachを利用した乳形質関連遺伝子座の探索

  山地 啓太, 石井 淳, 細川 大介, 松本 大和, 笹崎 晋史, 大山 憲二, 万年 英之

  第12回日本動物遺伝育種学会大会, Nov. 2011, Japanese, 日本動物遺伝育種学会, 広島, Domestic conference

  Poster presentation


• 大容量データによる黒毛和種雌牛の繁殖形質についての遺伝率および反復率の推定

  松田 洋和, 谷口 幸雄, 勝田 智博, 大山 憲二, 祝前 博明

  第49回肉用牛研究会, 2011, Japanese, Domestic conference

  Oral presentation


• 神戸大学農学部における食の教育

  大山 憲二

  大阪府立大学「食」フォーラム, 2011, Japanese, Domestic conference

  [Invited]

  Nominated symposium


• 黒毛和種の肥育後期における腰最長筋の筋肉内脂肪および脂肪酸組成の経時的変化

  小栗 佑介, 水野 雄仁, 本多 健, 万年 英之, 石田 孝史, 祝前 博明, 久下 志朗, 齋藤 邦彦, 大山 憲二

  日本畜産学会第114回大会, 2011, Japanese, Domestic conference

  Poster presentation


• 黒毛和種集団におけるsliding window approachを利用した枝肉形質関連遺伝子座の探索

  細川 大介, 石井 淳, 山地 啓太, 笹崎 晋史, 大山 憲二, 万年 英之

  第11回日本動物遺伝育種学会大会, Oct. 2010, Japanese, 日本動物遺伝育種学会, 白河, Domestic conference

  Poster presentation


• Polymorphisms of fat metabolism related genes and association between genotypes and carcass traits in Japanese Black cattle

  Narukami Takahiro, Bessyo Takuya, Tanaka Atsuko, Sasazaki Shinji, Oyama Kenji, Mannen Hideyuki

  32th International Conference on Animal Genetics, Jul. 2010, English, International Society for Animal Genetics, Edinburgh, UK, International conference

  Poster presentation


• Development of discrimination markers between Japanese domestic and imported beef based on a bovine 50K SNP array

  Sasazaki Shinji, Hosokawa Daisuke, Ishihara Rie, Aihara Hiroki, Oyama Kenji, Mannen Hideyuki

  32th International Conference on Animal Genetics, Jul. 2010, English, International Society for Animal Genetics, Edinburgh, UK, International conference

  Poster presentation


• 黒毛和種におけるDGAT1遺伝子多型の枝肉形質に対する効果

  鳴神 貴太, 笹崎 晋史, 大山憲二, 庄司 則章, 中嶋 宏明, 万年 英之

  第112回日本畜産学会大会, Mar. 2010, Japanese, 日本畜産学会, 明治大学, Domestic conference

  Oral presentation


• 和牛における育種の現状と展開

  大山 憲二

  平成22年度問題別研究会, 2010, Japanese, Domestic conference

  [Invited]

  Nominated symposium


• 黒毛和種における脂肪酸組成の遺伝率推定に関する検討

  永井 雅, 荒川 愛作, 谷口 幸雄, 祝前 博明, 野儀 卓哉, 大山 憲二

  平成22年度関西畜産学会大会, 2010, Japanese, Domestic conference

  Oral presentation


• Wagyu

  大山 憲二

  嶺南大学校(韓国)農業マイスター大学シンポジウム, 2010, Japanese, International conference

  [Invited]

  Nominated symposium


• Sampling method for estimating neutral allele frequency in a pedigreed population

  HONDA Takeshi, SASAZAKI Shinji, OYAMA Kenji, NOMURA Tetsuro, MUKAI Fumio

  9th World Congress of Genetics Applied to Livestock Production, 2010, English, International conference

  Poster presentation


• ホルスタイン種におけるFASN遺伝子の多型探索及び乳形質の効果

  稲田 聡士, 笹崎 晋史, 大山憲二, 真崎匡, 長谷部浩行, 阿部剛, 万年 英之

  第10回日本動物遺伝育種学会大会, Nov. 2009, Japanese, 日本動物遺伝育種学会, 群馬会館, Domestic conference

  Poster presentation


• ホルスタイン種における枝肉形質に関る遺伝子多型の効果

  鳴神 貴太, 笹崎 晋史, 大山 憲二, 野儀 卓哉, 万年 英之

  第111回日本畜産学会大会, Sep. 2009, Japanese, 日本畜産学会, 琉球大学, Domestic conference

  Oral presentation


• 和牛の来た道、進む道

  大山 憲二

  神戸大学全学統一公開講座, 2009, Japanese, Domestic conference

  Public discourse


• 黒毛和種繁殖雌牛の近年の泌乳量

  辻 絵美, 本多 健, 久下 志朗, 大山 憲二

  日本畜産学会第111回大会, 2009, Japanese, Domestic conference

  Oral presentation


• Secret of Wagyu

  大山 憲二

  FOODEX JAPAN 2009, 2009, English, International conference

  [Invited]

  Nominated symposium


• 黒毛和種における外貌記載法審査と繁殖形質との遺伝的関連性

  大山 憲二, 西川 隆清, 勝田 智博, 穴田 勝人, 向井 文雄

  日本畜産学会第104回大会講演要旨 p.80, 2005, Japanese, 日本畜産学会, 東京大学, Domestic conference

  Oral presentation


• 血縁関係を考慮した黒毛和種種雄牛の最適な供用頻度の導出

  野島 正成, 大山 憲二, 福島 護之, 穴田 勝人, 向井 文雄

  日本畜産学会第104回大会講演要旨 p.83, 2005, Japanese, 日本畜産学会, 東京大学, Domestic conference

  Oral presentation


• 毛和種における外貌形質と繁殖形質との遺伝的関連性

  西川 隆清, 大山 憲二, 穴田 勝人, 勝田 智博, 向井 文雄

  第103回日本畜産学会, 2004, Japanese, 日本畜産学会, 東京, Domestic conference

  Oral presentation


• 兵庫系黒毛和種（但馬牛）集団のジーンドロッピング法による系統分類の試み

  福島 護之, 坂瀬 充洋, 野田 昌伸, 武田 和士, 上野 悟, 本多 健, 大山 憲二, 向井 文雄

  第42回肉用牛研究会, 2004, Japanese, 肉用牛研究会, 東京, Domestic conference

  Oral presentation


• 黒毛和種における外貌形質と繁殖形質との遺伝的関連性

  西川 隆清, 大山 憲二, 穴田 勝人, 勝田 智博, 向井 文雄

  第103回日本畜産学会大会, 2004, Japanese, 日本畜産学会, 東京農工大学, Domestic conference

  Oral presentation


• Selective genotyping for marker assisted selection in Japanese Black cattle.

  SASAZAKI S, ITOH K, URATSUJI H, MANNEN Hideyuki, OYAMA Kenji, SHOJO Masayuki, MUKAI Fumio, TSUJI S

  未記入, 2004, English, 未記入, 未記入, International conference

  Oral presentation


• 黒毛和種繁殖雌牛集団におけるセレクティブ・ジェノタイピング

  浦辻 秀弥, 笹崎 晋司, 万年 英之, 大山 憲二, 庄條 昌之, 向井 文雄, 辻 荘一

  日本動物遺伝育種学会大会第4回大会, Nov. 2003, Japanese, 日本動物遺伝育種学会, 明治大学生田校舎, Domestic conference

  Oral presentation


• ウシ体脂肪の脂肪酸組成に影響する遺伝的要因

  宇津木 健司, 谷口 雅章, 万年 英之, 大山 憲二, 辻 荘一

  日本動物遺伝育種学会大会第4回大会, Nov. 2003, Japanese, 日本動物遺伝育種学会, 明治大学生田校舎, Domestic conference

  Oral presentation


• 黒毛和種繁殖雌牛の登録審査時に記録される外貌諸形質間の遺伝的関連性

  勝田 智博, 大山 憲二, 西川 隆清, 西郷 由香里, 穴田 勝人, 向井 文雄

  第101回日本畜産学会大会, Mar. 2003, Japanese, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, Domestic conference

  Oral presentation


• 黒毛和種繁殖雌牛の登録審査時に記載される外貌諸形質と枝肉形質間の遺伝的関連性

  大山 憲二, 勝田 智博, 西川 隆清, 西郷 由香里, 穴田 勝人, 向井 文雄

  第101回日本畜産学会大会, Mar. 2003, Japanese, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, Domestic conference

  Oral presentation


• 黒毛和種における種雄牛の供用パターンが集団構造に与える影響

  野島 正成, 大山 憲二, 福島 護之, 穴田 勝人, 向井 文雄

  第101回日本畜産学会大会, Mar. 2003, Japanese, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, Domestic conference

  Oral presentation


• 種雄牛の供用方法が但馬牛の集団構造に与える影響

  野島 正成, 大山 憲二, 福島 護之, 穴田 勝人, 向井 文雄

  第102回日本畜産学会, 2003, Japanese, 日本畜産学会, 岐阜大学, Domestic conference

  Oral presentation


• 種雄牛の供用方法が但馬牛の集団構造に与える影響

  野島 正成, 大山 憲二, 福島 護之, 穴田 勝人, 向井 文雄

  第101回日本畜産学会大会講演要旨, 2003, Japanese, 日本畜産学会, つくば, Domestic conference

  Oral presentation

• 動物の行動と管理学会


• 肉用牛研究会


• 日本動物遺伝育種学会


• 関西畜産学会


• 日本畜産学会

• 黒毛和種肥育牛における疾病の遺伝性と発症予測に関する研究

  大山 憲二

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2023 - 31 Mar. 2027


• Health Monitoring of Grazing Cattle by Time Series Analysis of Interactions

  大川 剛直, 太田 能, 大山 憲二

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Kobe University, 05 Apr. 2021 - 31 Mar. 2026

  本研究では、放牧牛に装着した無線センサタグを利用して得られる精緻な牛間インタラクションの時系列変化を分析することで、個別の牛から計測される運動情報や生体情報だけでは実現が困難であった高感度なヘルスモニタリング（ストレス検知、発情検知、健康状態把握、疾病・負傷発見）手法の開発を目的としている。令和3年度の主要な研究実績は以下の通りである。 放牧牛同士のインタラクションを抽出するためには、牛の位置を的確に把握することが求められる。そこで、牛間相互通信による位置推定の実現に向けて、双方向通信機能を有するBLE送受信デバイスの仕様について検討・試作を進めるとともに、受信電波強度データをもとに個々の放牧牛の位置推定が可能な手法について提案した。この手法では、放牧場のような実環境においてデータ欠損が頻繁に発生することに留意し、ゾーン分けによって欠損影響の削減を試みている。 また、詳細なインタラクション情報を得るためには、それぞれの時刻において牛がどのような行動を行っているかを把握する必要があることに着目し、BLE送受信デバイスに内蔵した加速度センサから得られる時系列データをもとに、個々の牛の行動（歩行、採食、横臥、佇立など）を推定するための手法について、基礎的な検討を実施した。 さらに、実際の牛における異常状態を把握するため、定期的な血液検査を実施し、データ蓄積を行った。具体的には、ストレス関連ホルモンであるコルチゾールの血中値をもとにストレスの度合いを定量化するとともに、一般生化学検査を通して、各個体の健康状態に関するデータを継続的に収集した。


• ウシ脂肪交雑形成機構の解明に向けた候補遺伝子変異の網羅的検証

  笹崎 晋史, 川口 芙岐, 万年 英之, 大山 憲二

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2022 - 31 Mar. 2025


• ゲノムリシーケンスデータに基づくウシ脂肪交雑を支配する遺伝子変異の網羅的探索

  笹崎 晋史

  学術研究助成基金助成金／基盤研究(C), Apr. 2018 - Mar. 2021

  Competitive research funding


• 但馬牛の長期的な遺伝的多様性維持における系統造成の有効性

  大山 憲二

  学術研究助成基金助成金／基盤研究(C), Apr. 2017 - Mar. 2020, Principal investigator

  Competitive research funding


• ウシ体脂肪の脂肪酸組成に関る責任遺伝子群の総合的解明

  万年 英之

  科学研究費補助金／基盤研究(B), Apr. 2016 - Mar. 2020

  Competitive research funding


• 放牧牛におけるインタラクション情報の取得によるコミュニティ分析とその応用

  大川 剛直

  学術研究助成基金助成金／挑戦的萌芽研究, Apr. 2015 - Mar. 2018

  Competitive research funding


• 放牧牛のインタラクション情報と生体・健康情報との関連性に関する分析と評価

  大山 憲二

  国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業 チーム型研究（CREST）, 2017, Principal investigator

  Competitive research funding


• 肉用子牛の体測定値推定技術を活用した母性能力向上のための研究開発・実証とその成果の普及

  大山 憲二

  公益社団法人畜産技術協会, 畜産・酪農生産力強化対策事業（繁殖性等向上対策）, 2017, Principal investigator

  Competitive research funding


• 近年におけるウシの繁殖性低下は経済形質の選抜によるものか

  万年 英之

  学術研究助成基金助成金／挑戦的萌芽研究, Apr. 2014 - Mar. 2016

  Competitive research funding


• 放牧牛のインタラクション情報と生体・健康情報との関連性に関する分析と評価

  大山 憲二

  国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業（CREST）, 2016, Principal investigator

  Competitive research funding


• 遺伝子発現量を基盤とした黒毛和種の不飽和脂肪酸に関与する遺伝子の探索

  大山 憲二

  科学研究費補助金／基盤研究(C), Apr. 2012 - Mar. 2015, Principal investigator

  Competitive research funding


• 遺伝的アルゴリズムによる黒毛和種集団の遺伝的多様性維持に関する研究

  大山 憲二

  科学研究費補助金／若手研究(B), 2005, Principal investigator

  Competitive research funding


• 黒毛和種遺伝子発現のDNAマイクロアレーによる解析

  辻 荘一, 大山 憲二, 万年 英之, 向井 文雄, 庄條 昌之, HARPER Greg

  日本学術振興会, 科学研究費助成事業, 萌芽研究, 神戸大学, 2002 - 2003

  家畜のゲノム研究は急速に発展し、ウシ全ゲノム配列が決定されようとしている。この段階に来て、家畜の経済的な形質に関連する全遺伝子を把握し、家畜の選抜や効率の良い生産を目指す学問が台頭してきた。それが、バイオインフォマティックスである。このバイオインフォマティックス研究にはマイクロアレーを利用した、網羅的・総合的な遺伝子解析が必要となる。我々は、オーストラリアのCooperative Research Center for Cattle and Beef Quality (CRCMQ)、CSIROと共同で、牛肉の肉質の改善に係わる遺伝子を発見するためのcDNAマイクロアレーの製作と、それを用いた分析に取り組んできた。実際には裾、神戸大学農学部附属農場にて、黒毛和種、ホルスタイン種、両者の一代雑種、夫々3頭を供試し、経時的にロース部分の筋肉(約1g)を生体より採取し、mRNAの分析、アミノ酸組成の分析、脂肪酸組成の分析等に取り組んだ。 mRNAの分析ではこれらの経時的に採取したサンプルと屠殺時に採取したサンプルとを9、000種類の遺伝子を貼り付けたcDNAマイクロアレーによって分析した。マイクロアレーの作成と分析はオーストラリア側が行った。その結果、肥育開始直後の10ヶ月齢付近で、品種間で著しく異なる遺伝子が、多数発見された。そのうちの一つは、以前から見当をつけていた遺伝子、Stearoyl coAdesaturase (SCD)で、この遺伝子が、牛肉の品質の指標になる事が改めて確認された。また、その他多数の遺伝子が発見されているので、今後はSCD以外の個々の遺伝子一つずつについて、Real-time PCR法などによって経時的に分析に、複数の遺伝子の経時的な動態が黒毛和種の肉質の改善にどのように係わっているかを明らかにしてゆく。この研究によって、何時の時期にどの遺伝子を指標にして調べれば、屠殺時の肉質の指標となるか、肉質改善の目標として、どのような遺伝子を対象にすれば肉質の改善が図れるかが明らかになろうとしていて、極めて期待の大きい成果が得られている。次いで、8頭の黒毛和種を供試し、ビタミンA濃度の肉質に関する影響を分析中で、ビタミンAの肉質に関わる遺伝子に及ぼす影響の発現解析が可能になる。

• 但馬牛博物館長

  Others, Apr. 2022


• 第25回 和牛育種・改良問題公開セミナー

  公益社団法人全国和牛登録協会

  Lecturer, 08 Jan. 2020


• 神戸大学学友会大阪クラブ・大阪凌霜クラブ定例会

  神戸大学学友会大阪クラブ・大阪凌霜クラブ

  Lecturer, 15 Oct. 2019


• 令和元年度第1回『食育シンポジウム』～農業体験して、食生活を考えよう！～

  農林水産省近畿農政局

  28 Jun. 2019


• 兵庫県立小野高校出張講義

  兵庫県立小野高校

  Lecturer, 13 Jun. 2019