Research activity information

• Potential of zoledronate for treating diffuse sclerosing osteomyelitis of the mandible in adult patients.

  Junya Kusumoto, Shungo Furudoi, Yumi Muraki, Moeka Warabi, Daisuke Takeda, Masaya Akashi

  INTRODUCTION: Diffuse sclerosing osteomyelitis (DSO) is a rare nonbacterial bone disease associated with recurrent pain and swelling, and its pathogenesis remains unknown. Despite the absence of an established treatment for DSO, bisphosphonates have recently been considered effective in managing this condition. However, the use of zoledronate is off-label, with limited reported cases. Therefore, this study aimed to investigate the effects of zoledronate on pain suppression in DSO. METHODS: This single-arm retrospective study evaluated adult patients diagnosed with mandibular DSO and treated with zoledronate. Patient demographics, pain suppression effect, recurrence, number of zoledronate administration, adverse reactions to zoledronate, and imaging findings were investigated. RESULTS: The study included 18 patients (median age of 59.5 years). Zoledronate effectively suppressed pain in all patients, with a median duration of effect onset of 1 day. Symptom recurrence was observed in 66.7% of patients, with a median time of 29 months from the first zoledronate administration to recurrence. Zoledronate was administered multiple times to 44.7% of patients. The median duration of response was 80 months for patients who experienced relief after a single administration of zoledronate compared to 32 months for those who received multiple administrations (p < 0.001). Adverse reactions, including flu-like symptoms, were observed in 72.2% of the patients, and no medication-related osteonecrosis of the jaw was observed during the follow-up period. CONCLUSIONS: Zoledronate is a relatively safe and effective treatment option for DSO of the mandible. Key Points • Zoledronate effectively suppresses pain in mandibular diffuse sclerosing osteomyelitis. • All condylar lesions recurred after zoledronate administration. • Patients with mandibular canal enlargement required multiple doses of zoledronate. • Adverse reactions to zoledronate administration were minor.

  Apr. 2025, Clinical rheumatology, English, International magazine

  Scientific journal


• Evaluation of Zoledronate for Pain Suppression in Diffuse Sclerosing Osteomyelitis of the Mandible: Protocol for a Prospective, Single-Arm Interventional Study.

  Junya Kusumoto, Shungo Furudoi, Daisuke Takeda, Megumi Kishimoto, Masaya Akashi

  INTRODUCTION: Diffuse sclerosing osteomyelitis (DSO) is a non-bacterial osteomyelitis that occurs infrequently and is characterized by recurrent severe pain, swelling of the mandible, and trismus. To date, no effective treatment has been established. Bisphosphonates have been suggested as an efficacious treatment for DSO; however, no prospective studies have examined the effect of bisphosphonate treatment in adults with DSO. This study aims to evaluate the effect of zoledronate on pain suppression in patients with DSO. METHODS: This is a single-center, exploratory, single-arm, prospective interventional study. The participants will include eight patients with DSO of the mandible. The primary endpoint is the change in pain levels before and after zoledronate administration. The secondary endpoints are assessing the efficacy of the clinical course, imaging changes, and safety monitoring. Patients will be enrolled if they are deemed eligible following screening tests and will receive only one 4.0 mg intravenous dose of zoledronate within seven days of enrollment. Study participants will be followed up at one, four, 12, 24, and 48 weeks post-dose or at discontinuation after zoledronate administration. CONCLUSION: This study is the first prospective trial to evaluate the effect of administering a single dose of zoledronate on pain suppression in patients with DSO. We believe this study will lead to the conclusion that zoledronate is an effective treatment for DSO.

  Mar. 2025, Cureus, 17(3) (3), e80739, English, International magazine

  Scientific journal


• Zymosan-Treated SKG Mice: Assessing Effects of Systemic Inflammation on the Temporomandibular Joint.

  Kaito Uryu, Yasumasa Kakei, Sho Sendo, Ikuko Goto, Kengo Akashi, Keisuke Nishimum, Tatsuya Shirai, Junya Hirota, Daisuke Takeda, Manabu Shigeoka, Akira Kimoto, Takumi Hasegawa, Jun Saegusa, Masaya Akashi

  OBJECTIVES: The effects of systemic inflammation on the temporomandibular joint (TMJ) are poorly understood. This study aimed to establish a mouse model to study the effects of systemic inflammation on the TMJ. MATERIALS AND METHODS: SKG mice, a BALB/c strain with spontaneous onset of rheumatoid arthritis-like symptoms due to a spontaneous point mutation (W163C) in the gene encoding the SH2 domain of ZAP-70, were treated with zymosan (β-1,3-glucan). Synovitis, bone erosion, and cartilage damage in the TMJ were evaluated using established scores for animal models of inflammatory arthritis. Myeloperoxidase-positive areas and numbers of tartrate-resistant acid phosphatase (TRAP)-positive cells were compared between naive and zymosan-treated SKG mice. Correlations between TMJ inflammation scores and clinical scores for extremities were also assessed. RESULTS: There were significant differences in TMJ inflammation scores, including synovitis, bone erosion, and cartilage damage, between naive and high-dose zymosan-treated mice. There were significant differences in myeloperoxidase-positive areas and numbers of TRAP-positive cells between naive and zymosan-treated mice. There were significant correlations between TMJ inflammation scores and clinical scores for extremities. CONCLUSIONS: Systemic administration of zymosan efficiently induces TMJ inflammation in SKG mice. Zymosan-treated SKG mice offer a useful tool to investigate the effects of systemic inflammation on the TMJ.

  Dec. 2024, Oral diseases, English, International magazine

  Scientific journal


• Predictive factors in difficult postoperative airway management of severe odontogenic deep neck infection.

  Eiji Iwata, Go Inokuchi, Masakazu Kawakami, Taiki Matsui, Junya Kusumoto, Akira Tachibana, Masaya Akashi

  In this study, we aimed to identify risk factors that predict the postoperative need for advanced or prolonged airway management in patients with severe odontogenic deep neck infections (DNIs). This retrospective case-control study included patients of both sexes aged ≥ 18 years who had undergone surgical drainage including debridement of necrotic tissues of odontogenic deep neck abscesses and necrotizing soft tissue infection under general anesthesia between April 2016 and September 2023 at a single center. The patients' characteristics, laboratory tests, and computed tomography (CT) findings were analyzed and compared between the difficult postoperative airway group, which required prolonged intubation or tracheostomy, and the short-term intubation group. Statistical significance was set at P < 0.05. Sixty-four patients required surgical drainage including debridement under general anesthesia. Of them, 7 (10.9%) patients were included in the difficult postoperative airway group. In addition to increased inflammatory markers, the presence of arytenoid edema among laryngeal edema and retro- and parapharyngeal space abscesses on preoperative CT images were identified as risk factors. The presence of pharyngeal space abscesses was significantly associated with laryngeal edema, and the intubation period was longer in patients with more elements relevant to these two factors. Thus, the presence of pharyngeal space abscesses and degree of laryngeal edema on preoperative CT images can be used to predict the complexity of postoperative airway management. Our results suggest that tracheostomy is preferable for patients with retropharyngeal space abscesses, and that patients with parapharyngeal space abscesses and laryngeal edema are desirable to undergo prolonged intubation.

  Dec. 2024, Odontology, English, Domestic magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Dd診断力てすと 右下顎臼歯部の腫瘤

  筧 康正, 明石 昌也

  (株)デンタルダイヤモンド社, Dec. 2024, DENTAL DIAMOND, 49(16) (16), 19 - 20, Japanese


• Effect of Statins on Patients With Osteoradionecrosis of the Jaw.

  Junya Kusumoto, Yumi Muraki, Akiko Sakakibara, Shungo Furudoi, Masaya Akashi

  BACKGROUND: Osteoradionecrosis of the jaw (ORN) is a late complication of radiation therapy. Radiation-induced fibrosis is the most likely mechanism for developing ORN, and statins are effective against radiation-induced fibrosis. However, no reports have indicated the direct effectiveness of statins in treating ORN. PURPOSE: This study aimed to measure the association between statin exposure and ORN disease resolution. STUDY DESIGN, SETTING, SAMPLE: This retrospective cohort study included patients with ORN diagnosed between January 2008 and December 2020 at the Hospital's Department of Oral and Maxillofacial Surgery. Patients who were immunocompromised or followed up for < 6 months were excluded. PREDICTOR VARIABLE: The predictor variable was statin exposure, which was defined as the use of statins for dyslipidemia. MAIN OUTCOME VARIABLE: The main outcome variable was ORN disease progression status (good prognosis). Patients who showed full recovery and improvement were included in the good prognosis group, and those who showed invariance and deterioration were included in the poor prognosis group. COVARIATES: We analyzed the clinicodemographic including the age of onset, sex, history of smoking, alcohol consumption, history of chemotherapy, tumor site, association with dental treatment, location (maxilla or mandible), the time to ORN onset from radiation therapy, and stage of ORN, and treatment characteristics including hyperbaric oxygen therapy, long-term macrolide administration, and sequestrectomy. ANALYSES: We analyzed the association between statin exposure or covariates and time to ORN improvement using bivariate and multivariate Cox regression. The significance level was set at P = .05. RESULTS: We analyzed 102 patients, and the improvement rate was 32.4%. The favorable prognostic factors were statin exposure (adjusted hazard ratio [HR], 3.71; 95% confidence interval [CI], 1.62 to 8.50; P = .002), onset in the maxilla (HR, 2.15; 95% CI, 1.02 to 4.55; P = .045), and stage 1 of ORN (HR, 2.65; 95% CI, 1.20 to 5.83; P = .016). CONCLUSION AND RELEVANCE: In this study, statin exposure, onset in the maxilla, and stage 1 of Lyons's classification were favorable prognostic factors for ORN. Although this study's findings were insufficient to recommend statin use for ORN, statins may be a novel and effective treatment for ORN.

  Dec. 2024, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 82(12) (12), 1585 - 1595, English, International magazine

  Scientific journal


• Dental dysplasia in childhood cancer survivors: a case series of permanent tooth abnormalities.

  Yumi Muraki, Atsushi Shioyasono, Mika Nishii, Daisuke Takeda, Junya Kusumoto, Masaya Akashi

  PURPOSE: This study was performed to investigate the association of dental dysplasia with childhood cancer. We examined the occurrence of agenesis, microdontia, and enamel changes of permanent teeth in pediatric cancer survivors. METHODS: Seventy-six patients with pediatric cancer and hematologic diseases were referred to our department for the first time from October 2005 to December 2019. Of these, 17 patients who presented for a checkup in our department fulfilled the study criteria and were analyzed. Clinical examinations and panoramic radiographs were performed to investigate dental dysplasia of permanent teeth. A total of 34 developmental abnormalities (18 missing teeth, 16 cases of microdontia) occurred in 5 patients. The patients' medical records were also analyzed to assess the relationships of specific types of dysplasia with the treatment duration and cumulative drug dose administered. RESULTS: All five pediatric cancer survivors had dental dysplasia; all five had tooth agenesis, and four had microdontia. All five patients were < 4 years of age and had undergone high-dose chemotherapy. CONCLUSION: In this study, childhood cancer survivors who received high-dose chemotherapy before age 4 years experienced the dental dysplasia, including tooth agenesis, microdontia. These findings highlight the need for careful dental monitoring and early intervention in pediatric patients undergoing high-dose chemotherapy. CLINICAL TRIAL NUMBER: This research was conducted in accordance with the Declaration of Helsinki and approved by the Medical Ethics Committee of the Medical University of Kobe (protocol code No. B230066, 1 September 2023).

  Nov. 2024, Oral and maxillofacial surgery, 29(1) (1), 9 - 9, English, International magazine

  Scientific journal


• Relationship between bone union and degree of bone marrow fibrosis at resection margins of advanced mandibular ORN.

  Hiroaki Ohori, Eiji Iwata, Chihiro Ichikawa, Manabu Shigeoka, Yoshiaki Tadokoro, Daisuke Takeda, Junya Kusumoto, Takumi Hasegawa, Masaya Akashi

  BACKGROUND: The pathological evaluation of cancellous bone at resection margins of mandibular osteoradionecrosis (ORN) has not been well elucidated. Here, we developed a unique classification system for evaluating the degree of bone marrow fibrosis, one of most common pathological features, in patients with mandibular ORN, based on which we investigated its relationship with treatment outcome. METHODS: This study included 15 patients who underwent mandibulectomy and free fibula osteocutaneous flap reconstruction. The extent of mandibulectomy was determined, with safety margins of approximately 10 mm from the apparent osteolytic areas on preoperative computed tomography image. Special staining was performed on thin sections from center of the osteolytic areas (medial area) and bilateral resection margins, and the degree of bone marrow fibrosis was evaluated and investigated its relationship with presence of bone union as a treatment outcome. RESULTS: The degree of bone marrow fibrosis of medial area was significantly higher than those of resection margins. Although most resection margins had collagen fibers which indicate severe fibrosis, all transferred fibula flaps achieved bone union. CONCLUSION: When mandibulectomy is performed with safety margins of approximately 10 mm from the apparent osteolytic areas, all transferred fibula flaps achieved bone union regardless of the degree of bone marrow fibrosis at resection margin. In other words, the association between severe bone marrow fibrosis at resection margins and treatment outcome was not seen. CLINICAL RELEVANCE: Setting safety margins of approximately 10 mm may achieve bone union, but further study is needed.

  Nov. 2024, Clinical oral investigations, 28(11) (11), 626 - 626, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 周術期のオーラルマネジメントによる外科感染症の予防 歯とHeartとの関連

  楠元 順哉, 片山 めぐみ, 村木 友美, 明石 昌也

  (一社)日本外科感染症学会, Oct. 2024, 日本外科感染症学会雑誌, 21(1) (1), 198 - 198, Japanese


• The Radiographic Characteristics of Mandibular Wisdom Teeth That Can Cause Severe Deep Neck Infection.

  Eiji Iwata, Junya Kusumoto, Takumi Hasegawa, Akira Tachibana, Masaya Akashi

  PURPOSE: Mandibular wisdom teeth can occasionally cause infections, which can progress to severe deep neck infections (DNIs) including deep neck abscesses or necrotizing soft tissue infections, which are fatal. This study aimed to identify the radiographic characteristics of mandibular wisdom teeth that developed severe DNIs. METHODS: This study included patients who were admitted for the treatment of severe mandibular wisdom tooth infection between July 2012 and June 2024 at a single center. Patient characteristics, clinical data, and radiographic findings were analyzed and compared between the severe DNI group and mild DNI group including patients with cellulitis or superficial abscess. P < 0.05 was considered significant. RESULTS: Nineteen of 42 patients (45.2%) were included in the severe DNI group. The multivariate analysis showed that the highest odds ratio (OR) was for the presence of a radicular cyst (OR=17.7), followed by the presence of a dentigerous cyst (OR =14.5). The most common mandibular wisdom tooth with a dentigerous cyst in patients with severe DNIs was inverted according to Winter's classification and type IIIC in the Pell and Gregory classification. CONCLUSION: Radiographic characteristics associated with severe DNIs included the presence of radicular and dentigerous cysts in the mandibular wisdom teeth. Especially in dentigerous cysts, deeply impacted teeth should be taken attention.

  Oct. 2024, Cureus, 16(10) (10), e70791, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 下顎びまん性硬化性骨髄炎に対してゾレドロン酸は有効な治療薬となりうるか?

  楠元 順哉, 古土井 春吾, 村木 友美, 武田 大介, 明石 昌也

  (NPO)日本口腔科学会, Sep. 2024, 日本口腔科学会雑誌, 73(2) (2), 195 - 195, Japanese


• ヒト下歯槽神経のバリア制御に関連する分子の解析

  平岡 佑二郎, 松村 恵実, 筧 康正, 武田 大介, 重岡 学, 木本 明, 長谷川 巧実, 明石 昌也

  (NPO)日本口腔科学会, Sep. 2024, 日本口腔科学会雑誌, 73(2) (2), 113 - 113, Japanese


• 歯の喪失は65歳高齢者の早期要介護発生リスクと関係するか?

  筧 康正, 白井 達也, 長谷川 巧実, 明石 昌也

  (NPO)日本口腔科学会, Sep. 2024, 日本口腔科学会雑誌, 73(2) (2), 216 - 216, Japanese


• What Is the Appropriate Antibiotic Administration During Tooth Extractions in Patients Receiving High-Dose Denosumab?

  Eiji Iwata, Takumi Hasegawa, Hiroaki Ohori, Toshiya Oko, Tsutomu Minamikawa, Daisuke Miyai, Masaki Kobayashi, Naoki Takata, Shungo Furudoi, Junichiro Takeuchi, Kosuke Matsumoto, Akira Tachibana, Masaya Akashi

  PURPOSE: Medication-related osteonecrosis of the jaw (MRONJ) occasionally occurs following tooth extractions in cancer patients receiving denosumab (Dmab). However, there are currently no established guidelines for perioperative antibiotic administration during tooth extraction in these patients. The primary objective was to develop guidelines for the dose and frequency of antibiotics during tooth extraction by investigating the correlation between the current status of antibiotic administration and the development of MRONJ. METHODS: This study included 68 cancer patients receiving high-dose Dmab who had tooth extractions between 2012 and 2022 at 10 hospitals. The relationship between the way of perioperative antibiotic administration and the development of MRONJ was analyzed. A P-value < .05 was considered significant. RESULTS: There was considerable variability across hospitals and surgeons regarding the type, dosage, and duration of antibiotic administration. Amoxicillin (AMPC) was the most commonly used antibiotic. Focusing exclusively on teeth extracted under AMPC administration, MRONJ developed in 21 out of 123 teeth (17.0%). No significant relationship was found between the development of MRONJ and the dosage or duration of perioperative AMPC administration. CONCLUSION: Perioperative antibiotic administration alone may not be sufficient to prevent MRONJ. Therefore, a single preoperative dose is likely adequate for effective and appropriate AMPC administration. .

  Aug. 2024, Cureus, 16(8) (8), e67237, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Prognostic value of a mandibular canal staging system for primary lesions in patients with lower gingival squamous cell carcinoma: a multicenter, retrospective study

  Nobuhiro Yamakawa, Masaya Okura, Takumi Hasegawa, Mitsunobu Otsuru, Hironori Sakai, Eiji Hirai, Shin Rin, Shin-ichi Yamada, Souichi Yanamoto, Yusuke Yokota, Masahiro Umeda, Hiroshi Kurita, Michihiro Ueda, Masaya Akashi, Tadaaki Kirita

  Springer Science and Business Media LLC, May 2024, International Journal of Clinical Oncology, 29(8) (8), 1122 - 1132

  Scientific journal


• 頸部膿瘍におけるドレナージ術後抜管困難症例の特徴

  岩田 英治, 楠元 順哉, 薄田 友理子, 橘 進彰, 明石 昌也

  (一社)日本有病者歯科医療学会, May 2024, 有病者歯科医療, 33(3) (3), 210 - 211, Japanese


• 重症感染症と歯性感染症を再考する 重症歯性感染症のスクリーニングと起因菌を含めた重症化因子

  楠元 順哉, 明石 昌也

  (一社)日本口腔感染症学会, May 2024, 日本口腔感染症学会雑誌, 31(1) (1), 23 - 31, Japanese


• Efficacy of perioperative oral care management in the prevention of surgical complications in 503 patients after pancreaticoduodenectomy for resectable malignant tumor: A multicenter retrospective analysis using propensity score matching

  Taihei Yamguchi, Kazuyo Mori, Yuka Kojima, Takumi Hasegawa, Junya Hirota, Masaya Akashi, Sakiko Soutome, Masako Yoshimatsu, Hiroshi Nobuhara, Yasuhiro Matsugu, Shinichiro Kato, Yasuyuki Shibuya, Hiroshi Kurita, Shin ichi Yamada, Hirokazu Nakahara

  Background: Pancreaticoduodenectomy has been associated with a high mortality rate and significant postoperative morbidity. Recently, perioperative oral care management has been reported to be effective in preventing postoperative pneumonia and surgical site infection. In this study, we examined the effect of perioperative oral care management in reducing complications after pancreaticoduodenectomy, including surgical site infection. Methods: This retrospective multicenter study included 503 patients who underwent pancreaticoduodenectomy at 8 facilities between January 2014 and December 2016. Among these, 144 received perioperative oral management by dentists and dental hygienists (oral management group), whereas the remaining 359 did not (control group). The oral care management program included oral health instructions, removal of dental calculus, professional mechanical tooth cleaning, removal of tongue coating, denture cleaning, instructions for gargling, and tooth extraction. The participants were matched using propensity scores to reduce background bias. Various factors were examined for correlation with the development of complications. Results: The incidence of organ/space surgical site infection was significantly lower in the oral management group than in the control group (8.0% vs 19.6%, P = .005). Multivariable logistic regression analysis revealed that hypertension and lack of perioperative oral management were independent risk factors for organ/space surgical site infection. Lack of perioperative oral management had an odds ratio of 2.847 (95% confidence interval 1.335–6.071, P = .007). Conclusion: Perioperative oral care management reduces the occurrence of surgical site infections after pancreaticoduodenectomy and should be recommended as a strategy to prevent infections in addition to antibiotic use.

  Apr. 2024, Surgery (United States), 175(4) (4), 1128 - 1133

  Scientific journal


• Efficacy of perioperative oral care management in the prevention of surgical complications in 503 patients after pancreaticoduodenectomy for resectable malignant tumor: A multicenter retrospective analysis using propensity score matching.

  Taihei Yamguchi, Kazuyo Mori, Yuka Kojima, Takumi Hasegawa, Junya Hirota, Masaya Akashi, Sakiko Soutome, Masako Yoshimatsu, Hiroshi Nobuhara, Yasuhiro Matsugu, Shinichiro Kato, Yasuyuki Shibuya, Hiroshi Kurita, Shin-Ichi Yamada, Hirokazu Nakahara

  BACKGROUND: Pancreaticoduodenectomy has been associated with a high mortality rate and significant postoperative morbidity. Recently, perioperative oral care management has been reported to be effective in preventing postoperative pneumonia and surgical site infection. In this study, we examined the effect of perioperative oral care management in reducing complications after pancreaticoduodenectomy, including surgical site infection. METHODS: This retrospective multicenter study included 503 patients who underwent pancreaticoduodenectomy at 8 facilities between January 2014 and December 2016. Among these, 144 received perioperative oral management by dentists and dental hygienists (oral management group), whereas the remaining 359 did not (control group). The oral care management program included oral health instructions, removal of dental calculus, professional mechanical tooth cleaning, removal of tongue coating, denture cleaning, instructions for gargling, and tooth extraction. The participants were matched using propensity scores to reduce background bias. Various factors were examined for correlation with the development of complications. RESULTS: The incidence of organ/space surgical site infection was significantly lower in the oral management group than in the control group (8.0% vs 19.6%, P = .005). Multivariable logistic regression analysis revealed that hypertension and lack of perioperative oral management were independent risk factors for organ/space surgical site infection. Lack of perioperative oral management had an odds ratio of 2.847 (95% confidence interval 1.335-6.071, P = .007). CONCLUSION: Perioperative oral care management reduces the occurrence of surgical site infections after pancreaticoduodenectomy and should be recommended as a strategy to prevent infections in addition to antibiotic use.

  Apr. 2024, Surgery, 175(4) (4), 1128 - 1133, English, International magazine

  Scientific journal


• Assessment of Patient Characteristics Influencing the Analgesic Effects of Ibuprofen Gargle After Mandibular Third Molar Extractions.

  Yasumasa Kakei, Takeshi Ioroi, Keiko Miyakoda, Takahiro Ito, Masahiko Kashin, Tatsuya Shirai, Takumi Hasegawa, Toshiyasu Sakane, Ikuko Yano, Masaya Akashi

  Introduction In our previous work, we investigated the analgesic effects of ibuprofen gargle after mandibular third molar extractions. However, a subsequent detailed review of individual patient data revealed variations in postoperative pain reduction among patients. Consequently, the present study was designed to conduct post-hoc subanalyses that identified factors contributing to variation in the analgesic response to ibuprofen gargle after third molar extractions. Materials and methods This study involved thirty-five Japanese patients from a prior randomized, double-blind, placebo-controlled, crossover study, which focused on the analgesic effects of ibuprofen gargle after mandibular third molar extractions. Participants were categorized as responders (n = 13) and non-responders (n = 22) based on the within-subject difference (ibuprofen-placebo, IP) of visual analog scale (VAS) changes. Baseline characteristics were compared, along with variables, such as age, sex, the reason for extraction, extraction site, Pell Gregory (space and depth) classification, Winter's classification, surgeon's experience, and surgery time. Baseline characteristics predicting responder status were examined using multivariate logistic regression. Results In the univariate analysis, variables such as age, sex, and baseline VAS scores with p-values <0.2 were evaluated using a stepwise approach. This analysis identified age (per -10 years) with an odds ratio of 4.163 (95% confidence interval (CI): 1.170-31.952, p = 0.0233) and sex (female) with an odds ratio of 9.977 (95% CI: 1.336-208.256, p = 0.0213) as significant predictors of responder status. Conclusions In young and female patients, ibuprofen gargle decreased postoperative pain after mandibular third molar extractions.

  Apr. 2024, Cureus, 16(4) (4), e57516, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Differential Photosensitivity of Fibroblasts Obtained from Normal Skin and Hypertrophic Scar Tissues.

  Junya Kusumoto, Masaya Akashi, Hiroto Terashi, Shunsuke Sakakibara

  It is unclear whether normal human skin tissue or abnormal scarring are photoreceptive. Therefore, this study investigated photosensitivity in normal skin tissue and hypertrophic scars. The expression of opsins, which are photoreceptor proteins, in normal dermal fibroblasts (NDFs) and hypertrophic scar fibroblasts (HSFs) was examined. After exposure to blue light (BL), changes in the expression levels of αSMA and clock-related genes, specifically PER2 and BMAL1, were examined in both fibroblast types. Opsins were expressed in both fibroblast types, with OPN3 exhibiting the highest expression levels. After peripheral circadian rhythm disruption, BL induced rhythm formation in NDFs. In contrast, although HSFs showed changes in clock-related gene expression levels, no distinct rhythm formation was observed. The expression level of αSMA was significantly higher in HSFs and decreased to the same level as that in NDFs upon BL exposure. When OPN3 knocked-down HSFs were exposed to BL, the reduction in αSMA expression was inhibited. This study showed that BL exposure directly triggers peripheral circadian synchronization in NDFs but not in HSFs. OPN3-mediated BL exposure inhibited HSFs. Although the current results did not elucidate the relationship between peripheral circadian rhythms and hypertrophic scars, they show that BL can be applied for the prevention and treatment of hypertrophic scars and keloids.

  Feb. 2024, International journal of molecular sciences, 25(4) (4), English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Segmental mandibulectomy for mandibular osteoradionecrosis in an older adult with underweight status

  Junya Kusumoto, Yuka Hayase, Yuriko Susukida, Takumi Sato, Akiko Sakakibara, Masaya Akashi

  Refractory osteomyelitis can lead to recurrent cellulitis and pathological fractures in patients with advanced mandibular osteoradionecrosis (ORN), the optimal treatment for which remains to be established. In addition, difficulties in intake due to trismus and severe pain caused by mandibular ORN may lead to malnutrition and underweight. Therefore, refeeding syndrome (RFS) should be considered when highly invasive surgical procedures are performed. In this report, we discuss the case of an 80-year-old woman with underweight status who had undergone radiotherapy for oropharyngeal malignant melanoma, following which she developed severe pain and a pathological fracture in the right mandible. Given her advanced age and cognitive decline, decisionmaking regarding the treatment plan was difficult. After repeated consultations with the patient and her family members, the treatment priorities were established as pain control, infection control, and increased mouth opening. A segmental mandibulectomy was performed. After nutritional intake was initiated postoperatively, a marked decrease in serum potassium and phosphorus levels was observed. Because there were no symptoms suggestive of RFS, imminent RFS was considered. Appropriate management in accordance with the National Institute for Health and Care Excellence guidelines prevented progression to RFS. Overall, surgery was successful in achieving the initial treatment objectives, and the patient exhibited a general improvement in quality of life. Careful perioperative management for RFS prevention should be considered necessary when performing surgical procedures for advanced mandibular ORN, especially in older adults.

  ELSEVIER SCIENCE INC, Jan. 2024, JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY MEDICINE AND PATHOLOGY, 36(1) (1), 58 - 63, English

  Scientific journal


• Transcutaneous carbon dioxide suppresses skeletal muscle atrophy in a mouse model of oral squamous cell carcinoma.

  Aki Sasaki, Daisuke Takeda, Hotaka Kawai, Yoshiaki Tadokoro, Aki Murakami, Nanae Yatagai, Satomi Arimoto, Hitoshi Nagatsuka, Masaya Akashi, Takumi Hasegawa

  Cancer cachexia causes skeletal muscle atrophy, impacting the treatment and prognosis of patients with advanced cancer, but no treatment has yet been established to control cancer cachexia. We demonstrated that transcutaneous application of carbon dioxide (CO2) could improve local blood flow and reduce skeletal muscle atrophy in a fracture model. However, the effects of transcutaneous application of CO2 in cancer-bearing conditions are not yet known. In this study, we calculated fat-free body mass (FFM), defined as the skeletal muscle mass, and evaluated the expression of muscle atrophy markers and uncoupling protein markers as well as the cross-sectional area (CSA) to investigate whether transcutaneous application of CO2 to skeletal muscle could suppress skeletal muscle atrophy in cancer-bearing mice. Human oral squamous cell carcinoma was transplanted subcutaneously into the upper dorsal region of nude mice, and 1 week later, CO2 gas was applied to the legs twice a week for 4 weeks and FFM was calculated by bioimpedance spectroscopy. After the experiment concluded, the quadriceps were extracted, and muscle atrophy markers (muscle atrophy F-box protein (MAFbx), muscle RING-finger protein 1 (MuRF-1)) and uncoupling protein markers (uncoupling protein 2 (UCP2) and uncoupling protein 3 (UCP3)) were evaluated by real-time polymerase chain reaction and immunohistochemical staining, and CSA by hematoxylin and eosin staining. The CO2-treated group exhibited significant mRNA and protein expression inhibition of the four markers. Furthermore, immunohistochemical staining showed decreased MAFbx, MuRF-1, UCP2, and UCP3 in the CO2-treated group. In fact, the CSA in hematoxylin and eosin staining and the FFM revealed significant suppression of skeletal muscle atrophy in the CO2-treated group. We suggest that transcutaneous application of CO2 to skeletal muscle suppresses skeletal muscle atrophy in a mouse model of oral squamous cell carcinoma.

  2024, PloS one, 19(4) (4), e0302194, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Gold Nanoparticles Enhance the Tumor Growth-Suppressing Effects of Cetuximab and Radiotherapy in Head and Neck Cancer In Vitro and In Vivo

  Takumi Sato, Yasumasa Kakei, Takumi Hasegawa, Masahiko Kashin, Shun Teraoka, Akinobu Yamaguchi, Ryohei Sasaki, Masaya Akashi

  Introduction: Head and neck squamous cell carcinoma (HNSCC) treatment includes surgery, radiotherapy, and immunotherapy with the aim of eradicating cancer cells without affecting normal tissues. HNSCC expresses epidermal growth factor receptor (EGFR) and cetuximab, an IgG1 monoclonal antibody targeting epidermal growth factor receptor, has been approved for the treatment of HNSCC. However, cetuximab has low reactivity and induces serious side effects. Gold nanoparticles (AuNPs) were reported to enhance the local antitumor effects of radiotherapy without damaging normal cells. Methods and Results: This study investigated the in vitro effects of single and combination therapy with AuNPs (1.0 nM), cetuximab (30 nM), and radiotherapy (4 Gy) on a human HNSCC cell line, HSC-3. Combination treatment of AuNPs + cetuximab + radiotherapy markedly reduced HSC-3 numbers and proliferation and enhanced apoptosis compared with single and double combination treatments. Furthermore, the in vivo combination treatment (AuNPs + cetuximab + radiotherapy) of a xenograft model of HSC-3 cells transplanted into nude mice (BALB/cAJcl-nu/nu) reduced the tumor volume compared with the controls. Scanning electron microscopy demonstrated the presence of AuNPs in tumor tissues and toxicity analysis indicated that AuNPs had no toxic effect on normal tissues. Conclusions: This study showed that AuNPs alone do not have a tumor-suppressing effect, but they sensitize tumors to radiotherapy and bind to cetuximab, leading to enhanced antitumor effects.

  MDPI AG, Dec. 2023, Cancers, 15(23) (23), 5697 - 5697

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Detection of bone marrow edema in differential diagnoses of odontogenic cysts using dual-energy computed tomography.

  Takumi Hasegawa, Satomi Arimoto, Izumi Saito, Nanae Yatagai, Aki Murakami, Aki Sasaki, Yoshiaki Tadokoro, Wakiko Tani, Kiyosumi Kagawa, Masaya Akashi

  PURPOSE: In this study, we prospectively investigated the relationship between bone marrow edema (BME) and odontogenic cysts and explored the possibility of using dual-energy computed tomography (DECT) as an auxiliary tool for the diagnosis of odontogenic cysts. METHODS: This cross-sectional study included 73 patients who underwent the DECT scan and surgery for odontogenic cysts or odontogenic tumors. The virtual noncalcium (VNCa) computed tomography (CT) values and CT values were measured at several sites. The predictor variable was diagnosis, and the other variables included age, sex, and sites. The primary outcome was VNCa CT value. Variables were tested using the chi-square test or the Kruskal-Wallis test. The VNCa CT and CT values were tested using the Scheffe test for multiple comparisons. All variables were analyzed as independent variables affecting the VNCa CT values around the lesion in the multiple regression analysis. RESULT: There were 35 men and 38 women. The mean patient age was 50.0 ± 19.5 years (range: 8-86). The VNCa CT values (- 6.2 ± 34.3) around the lesion in patients with RCs were significantly higher than those in patients with dentigerous cysts (- 44.4 ± 28.6) and odontogenic keratocysts (- 67.3 ± 19.5). In multiple regression analysis, the VNCa CT values around the lesion showed a significant positive correlation with histological results (regression coefficient: - 0.605, P < 0.001). CONCLUSION: The presence of BME is associated with radicular cysts, and DECT can be used as an auxiliary tool for radicular cyst diagnosis.

  Dec. 2023, Oral and maxillofacial surgery, 27(4) (4), 675 - 684, English, International magazine

  Scientific journal


• Tooth Loss as a Predictor of Long-Term Care Requirements in the Elderly: A Study in Kobe City, Japan.

  Yasumasa Kakei, Tatsuo Kagimura, Yasuji Yamamoto, Tohmi Osaki, Hiroyuki Kajita, Shinsuke Kojima, Hisatomo Kowa, Miyuki Kawabata, Takumi Hasegawa, Masaya Akashi, Yoji Nagai

  INTRODUCTION: The Kobe project, which utilizes prospective data from the national health insurance system, focuses on early detection and preventive strategies through the Frail Kenshin health check-up program. Previous research has underscored the correlation between tooth loss and the decline in physical and cognitive functions. In this study, using Kobe project data, we examined the link between remaining teeth and long-term care needs in individuals aged 64-65 years, with primary and secondary objectives involving various health parameters and quality of life. METHODS: We analyzed baseline data from a prospective study conducted alongside the Frail Check program for generally healthy individuals aged 64-65 years to examine the relationship between the number of remaining teeth and various health indicators. This study focused on citizens aged 64-65 years to identify those at risk of needing long-term care by the age of 65 years. RESULTS: Data from 1,530 participants were obtained, excluding eight individuals for specific reasons. At the end of the follow-up period, 41 (2.7%) individuals required support and 15 (1.0%) needed long-term care alone. The data revealed a significant association between the number of remaining teeth and the need for long-term care or support, as demonstrated by the Cochran-Armitage trend test (p<0.001). Although trends were noted for nutrition and total Cognitive Functional Instrument Self scores, they did not reach statistical significance. Additionally, a decrease in the number of remaining teeth was significantly associated with worse European Quality of Life Five Dimensions (EQ-5D-5L) visual analog scale scores, mobility, and regular activities (p<0.001). CONCLUSION: Tooth loss indicates the potential long-term care needs of older adults. Monitoring oral health is crucial for addressing care requirements.

  Dec. 2023, Cureus, 15(12) (12), e49851, English, International magazine

  Scientific journal


• Efficacy and safety of ibuprofen gargle for postoperative pain after mandibular third molar extraction: A phase II, placebo-controlled, double-blind, randomized crossover trial.

  Takeshi Ioroi, Yasumasa Kakei, Takahiro Ito, Tatsuya Shirai, Yutaro Okazaki, Takumi Hasegawa, Masaya Akashi, Ikuko Yano

  OBJECTIVE: This study was designed to evaluate the postoperative efficacy and safety of using an ibuprofen gargle as a pain management strategy for patients who have undergone mandibular third molar extraction. We also ensured that the quality of treatment was not compromised throughout the study. MATERIAL AND METHODS: Patients were randomized in a 1:1 ratio into two groups: the ibuprofen-placebo (IP) group and the placebo-ibuprofen (PI) group. On postoperative Day (POD) 1, the IP group initiated ibuprofen administration, while the PI group started taking placebo. On POD 2, the IP group switched to using placebo, whereas the PI group switched to ibuprofen. From PODs 3-5, both groups were prescribed ibuprofen gargle. The primary endpoint was within-subject visual analog scale (VAS) score before and 5 min after the first use of the ibuprofen or placebo gargle on PODs 1 and 2 (ΔVAS5_ibuprofen  - ΔVAS5_placebo ). The incidence and severity of adverse events were assessed using the Common Terminology Criteria for Adverse Events version 5.0 and a subjective rating scale. RESULTS: This study enrolled 40 patients. The within-subject VAS5 of the IP and PI groups were 1.25 ± 12.0 and -5.26 ± 8.93 mm, respectively. The treatment effect of ibuprofen gargle was -2.01 ± 10.62 mm (p = .246). None of the patients in each group presented with serious adverse events or clinically significant complications (including dry sockets) after extraction. Transient adverse events, such as throat tingling and oral discomfort (grade 1), were observed in each group. CONCLUSION: Ibuprofen gargle was safe but did not provide significant pain relief when used after mandibular third molar extraction.

  Nov. 2023, Clinical and experimental dental research, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Risk factors associated with prognosis of patients with medication-related osteonecrosis of the jaw.

  Yoshiaki Tadokoro, Takumi Hasegawa, Daisuke Takeda, Aki Murakami, Nanae Yatagai, Satomi Arimoro, Eiji Iwata, Izumi Saito, Junya Kusumoto, Masaya Akashi

  OBJECTIVES: Medication-related osteonecrosis of the jaw (MRONJ) is a severe adverse effect of antiresorptive and/or antiangiogenic agents. As the treatment application for MRONJ is controversial, we aimed to identify the risk factors for poor prognosis and to help determine appropriate management. METHODS: This study included 119 patients. Relevant clinical data were obtained for all the patients. In computed tomography images, osteosclerosis, osteolysis, cortical perforation (buccal or lingual), periosteal reaction, and sequestration were evaluated. RESULTS: Multivariate analyses showed statistically significant associations between poor prognosis in patients with MRONJ and conservative treatment alone (hazard ratio [HR] 1.89), osteolysis (HR 4.67), and the absence of sequestration (HR 5.33). CONCLUSIONS: Conservative treatment alone without clear objectives needs to be avoided, and osteolytic change could be the criteria for surgical intervention. As the boundary between the lesion and vital bone is indistinct, we recommend extensive surgery in cases with unpredictable sequestration.

  Nov. 2023, Head & neck, English, International magazine

  Scientific journal


• The Efficacy of Carbon Dioxide Paste in Alleviating Pain in Patients After Neck Dissection: Protocol for a Double-Blinded, Randomized Controlled Trial.

  Yoshiaki Tadokoro, Daisuke Takeda, Izumi Saito, Nanae Yatagai, Yasumasa Kakei, Masaya Akashi, Takumi Hasegawa

  BACKGROUND: Head and neck cancers that cause severe aesthetic and functional disorders normally metastasize to the cervical lymph nodes. Patients with cervical lymph node metastasis are undergoing neck dissection. Shoulder complaints are common after neck dissection, with patients reporting symptoms such as pain, weakness, shoulder droop, and disability. However, no safe and effective treatment is available for this condition at present. We will conduct a double-blinded, randomized controlled trial to evaluate the efficacy of carbon dioxide (CO2) paste in relieving pain in patients after neck dissection. OBJECTIVE: This will be the first clinical study to compare the efficacy of CO2 paste with placebo in relieving postoperative pain in patients who underwent neck dissection. METHODS: We will perform this trial at the Kobe University Hospital in Japan. Patients will be randomized 1:1 into the CO2 paste and control groups. Patients in the CO2 paste group will have the CO2 paste applied to the cervical surface skin for 10 minutes once per day for 14 consecutive days. The primary end point of the study is a change in the visual analog scale (VAS) scores of neck pain from baseline on day 1 (preapplication) to the end of drug application (day 15). Secondary end points include changes in the following parameters from baseline on day 1 to the end of drug application (day 15) or the study (day 29): neck pain VAS score (days 1-29), grip strength (days 1-15 and 1-29), VAS scores for subjective symptoms (the feeling of strangulation, numbness, swelling, and warmth in the neck and shoulder region) for days 1-15 and 1-29, whether the VAS score improved more than 30% (days 1-15), the arm abduction test (days 1-15 and 1-29), shoulder range of motion (abduction and flexion) for days 1-15 and 1-29, occurrence of skin disorders, and occurrence of serious side effects. Periodic monitoring will be conducted for participants during the trial. This study was approved by the certified review board of Kobe University. RESULTS: The intervention commenced in May 2021 and will continue until March 2024. The collected data will provide information on the efficacy of the CO2 paste treatment. The primary end point will be compared using the Wilcoxon test, with the 1-sided significance level set at 5%. Each evaluation item will be summarized. Secondary efficacy end points will be analyzed to provide additional insights into the primary analysis. Findings based on the treatment effects are expected to be submitted for publication in 2025. CONCLUSIONS: This trial will provide exploratory evidence of the efficacy and safety of CO2 paste in relieving pain in patients after neck dissection. TRIAL REGISTRATION: Japan Registry of Clinical Trials (jRCT) identifier: jRCTs051210028; https://jrct.niph.go.jp/en-latest-detail/jRCTs051210028. INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID): DERR1-10.2196/50500.

  Nov. 2023, JMIR research protocols, 12, e50500, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 「日本におけるフルカスタムデバイスの展望(最精度顎再建から咬合再建最適化に向けて)」 遊離腓骨皮弁による下顎再建 過去,現在,これから

  楠元 順哉, 明石 昌也

  (公社)日本口腔外科学会, Nov. 2023, 日本口腔外科学会雑誌, 69(11) (11), 499 - 508, Japanese


• Development of Cu application using dual-energy computed tomography for detecting medication-related osteonecrosis of the jaw.

  Kazuki Shigenaga, Satomi Arimoto, Masahiro Kubo, Takumi Sato, Yujiro Hiraoka, Daisuke Takeda, Takumi Hasegawa, Kiyosumi Kagawa, Masaya Akashi

  INTRODUCTION: The present study developed an application using dual-energy computed tomography (DECT) focused on Cu for detecting medication-related osteonecrosis of the jaw (MRONJ). MATERIALS AND METHODS: First, we performed two types of phantom studies using a Cu wire syringe and pig mandible with Cu wire to detect Cu on DECT. Second, DECT examinations of 44 patients with MRONJ were performed to compare lesion and normal bone sites using single-energy CT, DECT-virtual non-calcium (VNCa), and DECT-Cu applications. Quantitative analyses of VNCa CT and CT values were performed, and a cut-off value was calculated using receiver operating characteristic analysis. Third, we compared the Cu content in the MRONJ and normal bone groups using inductively coupled plasma atomic emission spectroscopy (ICP-AES). RESULTS: The material-specific differences in attenuation between the two different energies enabled the accurate separation of Cu from Ca in phantom studies. The sensitivity and specificity for single-energy CT, DECT-VNCa, and DECT-Cu applications were 97.7% and 2.3%, 86.4% and 81.8%, and 88.6% and 97.7%, respectively. Thus, VNCa CT values obtained on DECT-Cu application images showed the highest area under the curve value and maximal diagnostic efficacy in differentiating lesion sites from normal bone sites. On ICP-AES analyses, the Cu content was significantly higher in the MRONJ group than in the normal bone group. CONCLUSION: DECT-Cu application demonstrated better diagnostic performance in detecting MRONJ compared with single-energy CT or DECT-VNCa.

  Nov. 2023, Journal of bone and mineral metabolism, 41(6) (6), 865 - 876, English, Domestic magazine

  Scientific journal


• 当科における口腔癌癌患者の下顎の広範囲顎骨支持型装置に際し口腔前庭拡張術を要した11例

  高木 ひかる, 筧 康正, 白井 達也, 平岡 佑二郎, 有本 智美, 武田 大介, 木本 明, 長谷川 巧実, 明石 昌也

  (公社)日本顎顔面インプラント学会, Nov. 2023, Japanese Journal of Maxillo Facial Implants, 22(3) (3), 242 - 242, Japanese


• Photodynamic therapy with verteporfin accelerates apoptotic bleb formation in human ameloblastoma.

  Junya Yamashita, Akira Kimoto, Shun Teraoka, Yujiro Hiraoka, Daisuke Takeda, Yasumasa Kakei, Manabu Shigeoka, Takumi Hasegawa, Masaya Akashi

  OBJECTIVE: Although benign, ameloblastoma is a locally aggressive lesion in some patients and the development of additional treatments is needed. Verteporfin (VP) is a photosensitizer exhibiting considerable photocytotoxicity in various tumor cells. We aimed to investigate the effects of verteporfin photodynamic therapy (VP PDT) on ameloblastoma. METHODS: Eighteen patients who underwent surgery for ameloblastoma were randomly selected. We performed an immunohistochemical assessment to investigate the expression of low-density lipoprotein receptor (LDLR) and Yes-associated protein (YAP), targets of VP, in human ameloblastoma tissues and cultured human ameloblastoma cell line (HAM1). The effect of VP PDT on cell proliferation and apoptosis in HAM1 was analyzed. RESULTS: The expression of LDLR and YAP were detected in human ameloblastoma tissues and HAM1. LDLR expression was significantly higher in patients who had previously undergone surgery than in patients who were receiving it for the first time. The cytotoxic effect of the combination of low-concentration VP administration and laser irradiation was comparable to high-concentration VP administration with and without laser irradiation. The addition of laser irradiation to VP administration significantly accelerated apoptotic bleb formation compared with VP administration alone. CONCLUSION: VP PDT has the potential to become an additional treatment for large-sized ameloblastoma.

  Corresponding, Oct. 2023, Oral diseases, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Correction to: Denosumab-related osteonecrosis of the jaw after tooth extraction and the effects of a short drug holiday in cancer patients: a multicenter retrospective study (Osteoporosis International, (2021), 32, 11, (2323-2333), 10.1007/s00198-021-05995-3)

  T. Hasegawa, N. Ueda, Si Yamada, S. Kato, E. Iwata, S. Hayashida, Y. Kojima, M. Shinohara, I. Tojo, H. Nakahara, T. Yamaguchi, T. Kirita, H. Kurita, Y. Shibuya, S. Soutome, M. Akashi

  In Table 1 of this article, the data (18(46.2) and 54(55.7)) in the columns 2 and 3/row 48 (page 2326) headed “Drug holiday before tooth extraction, yes” were mistakenly listed. The correct expression is 10(40.0) and 18(38.3). In Table 1 of this article, the data (21(53.8) and 43(44.3)) in the column 2 and 3/row 49 (page 2326) headed “Drug holiday before tooth extraction, No” were mistakenly listed. The correct expression is 15(60.0) and 29(61.7). In Table 1 of this article, the data (P value: 0.347) in the column 4 /row 48 (page 2326) were mistakenly listed. The correct expression is 1.000. In Table 1 (continued) of this article, the data (24(61.5) and 51(52.6)) in the [column 2 and 3/row 26] (page 2327) headed “Preoperative antibiotics administration, yes” were mistakenly listed. The correct expression is 15(60.0) and 23(48.9). In Table 1 (continued) of this article, the data (15(38.5) and 46(47.4)) in the [column 2 and 3/row 27] (page 2327) headed “Preoperative antibiotics administration, No” were mistakenly listed. The correct expression is 10(40.0) and 24(51.1). In Table 1 (continued) of this article, the data (P value:0.446) in the [column 4 /row 28] (page 2327) were mistakenly listed. The correct expression is 0.460. (Table presented.) Errata Variable DRONJ P-value Present n (%) Absent n (%) Patients, n 25 (34.7) 47 (65.3) Stage 0 1 (4.0) - Stage 1 10 (40.0) - Stage 2 11 (44.0) - Stage 3 3 (12.0) - Sex Male 10 (40.0) 21 (43.1) 0.805 * Female 15 (60.0) 26 (56.9) Age Range (years) 44–82 41–85 Mean ± SD 66.3 ± 11.3 64.60 ± 12.2 0.534 ** Performance status 0 or 1 23 (92.0) 44 (93.6) 1.000 * 2 or 3 2 (8.0) 3 (6.4) Smoking history Yes 3 (12.0) 9 (19.1) 0.735* No 18 (72.0) 33 (70.2) Unknown 4 (16.0) 5 (10.6) Duration of oncologic doses of denosumab Range (months) 5–51 1–85 Mean ± SD 22.4 ± 13.5 13.7 ± 15.3 < 0.001** ≥ 18 months 16 (66.7) 10 (27.8) < 0.001* < 18 months 9 (33.3) 37 (72.2) Comorbidity or drug-induced risk factors Diabetes mellitus Yes 2 (8.0) 3 (6.4) 1.000* No 23 (92.0) 44 (93.6) Corticosteroid therapy Yes 4 (16.0) 8 (17.0) 1.000* No 21 (84.0) 39 (83.0) Additional chemotherapy Yes 17 (72.3) 34 (70.8) 0.787* No 8 (27.7) 13 (28.2) Type of cancer Breast cancer 8 (32.0) 12 (25.5) 0.171*** Prostate cancer 2 (8.0) 12 (25.5) Multiple myeloma 1 (4.0) 4 (8.5) Lung cancer 2 (8.0) 6 (12.8) Other 1 (4.0) 4 (8.5) Unknown 11 (44.0) 9 (19.1) Interval between tooth extraction and last denosumab injection Range (days) 1–283 2–272 Mean ± SD 66.0 ± 61.7 58.9 ± 63.5 0.367** Drug holiday before tooth extraction Yes 10 (40.0) 18 (38.3) 1.000* No 15 (60.0) 29 (61.7) Reason for tooth extraction Periapical periodontitis Yes 16 (64.0) 25 (56.9) 0.457* No 9 (36.0) 22 (43.1) P4 periodontitis Yes 3 (12.0) 10 (21.3) 0.521* No 22 (88.0) 37 (78.7) Pericoronitis Yes 4 (16.0) 2 (4.6) 0.173* No 21 (84.0) 45 (95.7) Jawbone Maxillary 10 (40.0) 23 (48.9) 0.881*** Mandibular 14 (56.0) 21 (44.7) Maxillary and mandibular 1 (4.0) 3 (6.4) Site of tooth extraction Anterior region 2 (8.0) 5 (10.6) 0.562*** Molar region 22 (88.0) 37 (78.7) Anterior and molar region 1 (4.0) 5 (10.6) Bone volume around tooth Adequate alveolar bone volume 19 (76.0) 31 (66.0) 0.432* Bone loss 6 (24.0) 16 (44.0) Pre-existing inflammation Yes 24 (96.0) 29 (61.7) 0.002* No 1 (4.0) 18 (38.3) Preoperative antibiotics administration Yes 15 (60.0) 23 (48.9) 0.460* No 10 (40.0) 24 (51.1) Number of teeth extracted Single 11 (44.0) 20 (42.6) 1.000* Multiple 14 (56.0) 27 (57.4) Additional surgical procedure Bone removal Yes 11 (44.0) 11 (23.4) 0.106* No 14 (56.0) 36 (76.6) Root amputation Yes 5 (20.0) 6 (12.8) 0.497* No 20 (80.0) 41 (87.2) Wound status after extraction Open 12 (48.0) 15 (31.9) 0.736*** Closed with suture 12 (48.0) 28 (59.6) Completely closed with relaxation incision or removal of bone edge 1 (4.0) 4 (8.5) *Fisher’s exact test, **Mann-Whitney U test, ***chi-squared test. DRONJ, denosumab-related osteonecrosis of the jaw; SD, standard deviation

  Oct. 2023, Osteoporosis International, 34(10) (10), 1823 - 1825

  Scientific journal


• Comparison of the 8th edition of TNM staging of oral cancer with the 7th edition and its prognostic significance using clinical depth of invasion and extranodal extension

  Yusuke Yokota, Takumi Hasegawa, Nobuhiro Yamakawa, Shin Rin, Mitsunobu Otsuru, Shin-ichi Yamada, Eiji Hirai, Yuichi Ashikaga, Kozo Yamamoto, Michihiro Ueda, Tadaaki Kirita, Masahiro Umeda, Masaya Akashi, Hiroshi Kurita, Yoichi Ohiro, Souichi Yanamoto, Masaya Okura

  Elsevier BV, Oct. 2023, Oral Oncology, 145, 106519 - 106519

  Scientific journal


• Correction: Is the LRINEC score useful for predicting necrotizing fasciitis as a complication of MRONJ?

  Eiji Iwata, Junya Kusumoto, Yuriko Susukida, Taiki Matsui, Naoki Takata, Takumi Hasegawa, Akira Tachibana, Masaya Akashi

  Sep. 2023, Journal of bone and mineral metabolism, 41(5) (5), 652 - 653, English, Domestic magazine


• Effect of Frequent Computed Tomography Examinations with Contrast Media on the Renal Function of Patients with Oral Squamous Cell Cancer and an Evaluation of Risk Factors for Post-Operative Chronic Kidney Disease

  Takumi Hasegawa, Aya Matsuda, Rika Amano, Izumi Saito, Daisuke Takeda, Yasumasa Kakei, Akira Kimoto, Akiko Sakakibara, Masaya Akashi

  PurposeThis retrospective study aimed to investigate the effect of frequent computed tomography (CT) examinations with contrast media on the renal function of patients with oral squamous cell cancer (OSCC) that underwent radical surgery, by using estimated glomerular filtration rate (eGFR); to identify risk factors of occurrence of post-operative chronic kidney disease (CKD) in these patients; and to explore the relationship between risk factors and occurrence of postoperative CKD during follow-up.MethodsHerein, 188 patients (107 male; 81 female) who underwent radical surgery for OSCC were included. We evaluated the risk factors for postoperative CKD after treatment, including demographic, perioperative, and postoperative factors by univariate and multivariate analyses. Patients were divided into post-operative CKD and control groups based on eGFR evaluation. Overall survival (OS) rates were compared between the groups.ResultseGFR decreased over time after treatment in both patient groups. Postoperative CKD was diagnosed in 56 (29.8%) patients. The average number of contrast-enhanced CT examinations was not an independent risk factor for postoperative CKD. However, lower hemoglobin on hospital discharge [odds ratio (OR) = 0.53], lower eGFR on hospital discharge (OR = 0.84), and common use of nonsteroidal anti-inflammatory drugs (OR = 48.79) were significant risk factors associated with postoperative CKD. The control group was associated with a better OS than the postoperative CKD group; however, this difference was not significant.ConclusionsClinicians should pay close attention to these risk factor of post-operative CKD during the management of patients with OSCC that undergo radical surgery and frequent follow-up CT examinations with contrast media.

  SPRINGER INDIA, Sep. 2023, JOURNAL OF MAXILLOFACIAL & ORAL SURGERY, English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Relationship of Mitochondrial-Related Protein Expression with the Differentiation, Metastasis, and Poor Prognosis of Oral Squamous Cell Carcinoma.

  Aki Murakami, Daisuke Takeda, Junya Hirota, Izumi Saito, Rika Amano-Iga, Nanae Yatagai, Satomi Arimoto, Yasumasa Kakei, Masaya Akashi, Takumi Hasegawa

  Mitochondrial dysfunction and respiratory function changes have been consistently associated with the initiation and progression of cancer. The purpose of this study was to retrospectively investigate the expression of mitochondrial tumor-suppressor and DNA-repair proteins in patients with oral squamous cell carcinoma (OSCC) and to evaluate the relationship between their expression and prognosis. We enrolled 197 patients with OSCC who underwent surgical resection between August 2013 and October 2018. Clinical, pathological, and epidemiological data were retrospectively collected from hospital records. The expression of peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α), mitochondrial transcription factor A, mitochondrial tumor suppressor gene 1, silent information regulator 3, and 8-hydroxyguanine DNA glycosylase was investigated using immunochemistry. The 3-year disease-specific survival (DSS) rates of patients showing positive expression of all selected proteins were significantly higher than those of patients showing a lack of expression. Multivariate analysis revealed that the expression of PGC-1α (hazard ratio, 4.684) and vascular invasion (hazard ratio, 5.690) can predict the DSS rate (p < 0.001). Low PGC-1α expression and vascular invasion are potential clinically effective predictors of the prognosis of OSCC.

  Aug. 2023, Cancers, 15(16) (16), English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Author Correction: Risk factors for pathological fracture in patients with mandibular osteoradionecrosis.

  Hiroaki Ohori, Eiji Iwata, Daisuke Takeda, Junya Kusumoto, Takumi Hasegawa, Masaya Akashi

  Jul. 2023, Scientific reports, 13(1) (1), 11216 - 11216, English, International magazine


• A Novel Imaging Technique for Analyzing Condylar Movement During Mastication in Patients With Jaw Deformities: Four-Dimensional Computed Tomography.

  Akira Kimoto, Junya Yamashita, Hiroaki Ohori, Noriyuki Negi, Toshinori Sekitani, Hiroki Komori, Atsushi Shioyasono, Chizu Tateishi, Takumi Hasegawa, Masaya Akashi

  OBJECTIVE: This study aimed to reveal characteristic condylar movements in patients with jaw deformities. STUDY DESIGN: Thirty patients with jaw deformities before surgery were enrolled and instructed to chew a cookie during 4-dimensional computed tomography (4DCT). The distance between the most anterior and posterior positions of the bilateral condyles on 4DCT images was measured and compared among patients with different skeletal classes. Correlations between the condylar protrusion and cephalometric values were also determined. RESULTS: The distances of condylar protrusion during mastication were significantly greater in the skeletal class II group than in the skeletal class III group (P=0.0002). Significant correlations were found between the distances of condylar protrusion during mastication and the sella-nasion-B point angle (r=-0.442, P=0.015), A point-nasion-B point angle (r=0.516, P=0.004), sella-nasion plane to ramus plane angle (r=0.464, P=0.01), sella-nasion plane to occlusal plane angle (r=0.367, P=0.047), and condylion-gonion length (r=-0.366, P=0.048). CONCLUSION: Motion analysis with 4DCT images revealed that condylar movement in patients with retrognathism was larger than in patients with mandibular prognathism. Skeletal structure was therefore correlated with condylar movement during mastication.

  Jul. 2023, The Journal of craniofacial surgery, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• LRINEC scoreは薬剤関連顎骨壊死の合併症としての壊死性筋膜炎の予測に有用か?

  岩田 英治, 楠元 順哉, 薄田 友理子, 橘 進彰, 明石 昌也

  (一社)日本有病者歯科医療学会, Jul. 2023, 有病者歯科医療, 32(4) (4), 191 - 192, Japanese


• TruMatch Reconstruction Systemを用いて行った遊離腓骨皮弁による下顎再建の特徴

  蓮井 友統, 楠元 順哉, 早瀬 悠夏, 明石 昌也

  (NPO)日本口腔科学会, Jul. 2023, 日本口腔科学会雑誌, 72(2) (2), 123 - 123, Japanese


• OHATスコアは膿胸患者の3ヵ月時死亡リスク評価に有用である 傾向スコアマッチング法を用いた後ろ向き観察研究

  岩田 英治, 橘 進彰, 八谷 奈苗, 松井 太輝, 楠元 順哉, 長谷川 巧実, 明石 昌也

  (NPO)日本口腔科学会, Jul. 2023, 日本口腔科学会雑誌, 72(2) (2), 158 - 158, Japanese


• 経皮的炭酸ガスペーストによるラット筋損傷モデルにおける瘢痕化防止と筋再生促進効果

  廣田 純也, 長谷川 巧実, 伊賀 利香, 八谷 奈苗, 黒澤 泉, 武田 大介, 筧 康正, 木本 明, 明石 昌也

  (NPO)日本口腔科学会, Jul. 2023, 日本口腔科学会雑誌, 72(2) (2), 166 - 166, Japanese


• Incidence of antiresorptive agent-related osteonecrosis of the jaw: A multicenter retrospective epidemiological study in Hyogo Prefecture, Japan

  Masanori Nashi, Hiromitsu Kishimoto, Masaki Kobayashi, Akira Tachibana, Motoo Suematsu, Shigeyoshi Fujiwara, Yoshiyuki Ota, Susumu Hashitani, Takeshi Shibatsuji, Tetsuya Nishida, Kazuma Fujimura, Shungo Furudoi, Yoshiki Ishida, Shoichiro Ishii, Tsuyoshi Fujita, Soichi Iwai, Takashi Shigeta, Takeshi Harada, Daisuke Miyai, Daisuke Takeda, Masaya Akashi, Kazuma Noguchi, Toshihiko Takenobu

  Background/purpose: The incidence of medication-related osteonecrosis of the jaw is increasing worldwide, mostly due to the use of antiresorptive agents (ARAs) such as bi-sphosphonate (BP) and denosumab (Dmab). However, the proportion of BP-related osteonecro-sis of the jaw (BRONJ) and Dmab-related osteonecrosis of the jaw (DRONJ) among all ARA-related osteonecrosis of the jaw (ARONJ) cases is not clear; this hinders appropriate treat-ment, recurrence-prevention planning, and avoidance of unnecessary Dmab withdrawal. More-over, the causative drug administered at each disease stage remains unknown. Therefore, we conducted a retrospective study of patients with ARONJ who visited oral and maxillofacial sur-gery departments at hospitals in Hyogo Prefecture, Japan, over 3 years to classify and compare patient characteristics with those having BRONJ and DRONJ. We sought to identify the propor-tion of DRONJ in ARONJ. Materials and methods: After excluding stage 0 patients, 1021 patients were included (471 high-dose; 560 low-dose). ARA treatment for bone metastases of malignant tumors and multi-ple myeloma was considered high dose, while that for cancer treatment-induced bone loss and osteoporosis was low dose. Results: Low doses of BP and Dmab accounted for >50% patients; the results differed from those in other countries. DRONJ accounted for 58% and 35% of high-dose and low-dose cases, respectively. Stage 3 ARONJ cases comprised 92 (19.5%) low-dose BRONJ, 39 (20.1%) high-dose BRONJ, 24 (30%) low-dose DRONJ, and 68 (24.5%) high-dose DRONJ. Eighty-nine patients who received switch therapy were divided into BRONJ or DRONJ, but there was no difference in the ratio of each stage compared to the non-switch therapy. Conclusion: To the best of our knowledge, this is the first study to clarify the proportion of BRONJ and DRONJ cases, causative drug, and its doses by disease stages. DRONJ accounted for approximately 30% of the ARONJ, approximately 60% of which was due to high doses. 2022 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons. org/licenses/by-nc-nd/4.0/).

  ELSEVIER TAIWAN, Jul. 2023, JOURNAL OF DENTAL SCIENCES, 18(3) (3), English

  Scientific journal


• Expression of JCAD and EGFR in Perineurial Cell-Cell Junctions of Human Inferior Alveolar Nerve.

  Yujiro Hiraoka, Megumi Matsumura, Yasumasa Kakei, Daisuke Takeda, Manabu Shigeoka, Akira Kimoto, Takumi Hasegawa, Masaya Akashi

  Although perineurium has an important role in maintenance of the blood-nerve barrier, understanding of perineurial cell-cell junctions is insufficient. The aim of this study was to analyze the expression of junctional cadherin 5 associated (JCAD) and epidermal growth factor receptor (EGFR) in the perineurium of the human inferior alveolar nerve (IAN) and investigate their roles in perineurial cell-cell junctions using cultured human perineurial cells (HPNCs). In human IAN, JCAD was strongly expressed in endoneurial microvessels. JCAD and EGFR were expressed at various intensities in the perineurium. In HPNCs, JCAD was clearly expressed at cell-cell junctions. EGFR inhibitor AG1478 treatment changed cell morphology and the ratio of JCAD-positive cell-cell contacts of HPNCs. Therefore, JCAD and EGFR may have a role in the regulation of perineurial cell-cell junctions.

  Corresponding, Jun. 2023, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 71(6) (6), 221554231182193 - 221554231182193, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Is the LRINEC score useful for predicting necrotizing fasciitis as a complication of MRONJ?

  Eiji Iwata, Junya Kusumoto, Yuriko Susukida, Taiki Matsui, Naoki Takata, Takumi Hasegawa, Akira Tachibana, Masaya Akashi

  INTRODUCTION: Necrotizing fasciitis as a complication of medication-related osteonecrosis of the jaw (MRONJ), which we named "ONJ-NF", has been sometimes reported. This study aimed to investigate the usefulness of the Laboratory Risk Indicator for Necrotizing Fasciitis (LRINEC) score for predicting ONJ-NF. MATERIALS AND METHODS: We included patients with acute MRONJ who required hospitalization at a single institution from April 2013 to June 2022. They were divided into two groups: patients with ONJ-NF and those with severe cellulitis as a complication of MRONJ, which we named "ONJ-SC." LRINEC scores were compared between the groups and the cut-off value of the score was set by creating a receiver operating characteristic curve. RESULTS: Eight patients with ONJ-NF and 22 patients with ONJ-SC were included. The LRINEC score was significantly higher in patients with ONJ-NF (median: 8.0 points, range 6-10 points) than in those with ONJ-SC (median: 2.5 points, range 0-6 points). A LRINEC score of ≥ 6 points had a sensitivity of 100.0%, a specificity of 77.3%, and an area under the curve of 0.97. Among 6 parameters of LRINEC score, only C-reactive protein (CRP) and white blood cell count (WBC) had significant differences between two groups. Most of the patients with ONJ-NF were rescued by antibiotic therapy and surgical drainage including debridement of necrotic tissues, but unfortunately, one patient did not survive. CONCLUSION: Our results suggested that the LRINEC score may be a useful diagnostic tool to predict ONJ-NF but valuating only CRP and WBC may be sufficient particularly in patients with osteoporosis.

  Jun. 2023, Journal of bone and mineral metabolism, English, Domestic magazine

  Scientific journal


• 埋伏歯の開窓牽引中に生じた頸部膿瘍の1例

  八谷 奈苗, 橘 進彰, 薄田 友理子, 船原 隆一郎, 岩田 英治, 松井 太輝, 楠元 順哉, 明石 昌也

  (一社)日本口腔感染症学会, May 2023, 日本口腔感染症学会雑誌, 30(1) (1), 68 - 69, Japanese


• ステージ1-2舌癌におけるT分類第8版の妥当性の検証と後発頸部リンパ節転移の予測因子

  船原 隆一郎, 古川 浩平, 松井 太輝, 長谷川 巧実, 梅田 正博, 明石 昌也

  過去10年間にUICC第7版でステージ1-2と診断して舌扁平上皮癌手術を行った92例(男性53例、女性39例:25～92歳、平均65歳)を対象に、T分類に腫瘍の深達度(DOI)の概念を導入した第8版の妥当性と好発頸部リンパ節転移の予測因子について検討した。その結果、第8版T分類はDOIや簇出(TB)と共に潜在性頸部リンパ節転移と密接な関連を認め、予後を推定するために臨床的に有用な分類法である。

  (公社)日本口腔外科学会, May 2023, 日本口腔外科学会雑誌, 69(5) (5), 233 - 240, Japanese


• 当科で新型コロナウイルス感染症に対してPCR検査を行った患者についての考察

  利川 誠将, 武田 大介, 長谷川 巧実, 明石 昌也

  (一社)日本口腔感染症学会, May 2023, 日本口腔感染症学会雑誌, 30(1) (1), 65 - 66, Japanese


• 頭頸部粘膜悪性黒色腫に対する陽子線治療+adjuvant Nivolumab療法

  瓜生 開人, 出水 祐介, 窪田 光, 美馬 正幸, 福光 延吉, 今村 善宣, 明石 昌也, 副島 俊典

  (一社)日本頭頸部癌学会, May 2023, 頭頸部癌, 49(2) (2), 234 - 234, Japanese


• ダプトマイシンの長期投与が奏功したMRSA感染を伴う放射線性下顎骨骨髄炎の1例

  西川 華子, 古土井 春吾, 伊賀 利香, 黒澤 泉, 筧 康正, 明石 昌也

  (一社)日本口腔感染症学会, May 2023, 日本口腔感染症学会雑誌, 30(1) (1), 60 - 60, Japanese


• Four-Dimensional Computed Tomographic Image Evaluation of the Change of Condylar Movement With and Without Implant-Supported Removable Prostheses in the Fibular Mandible: A Pilot Case Series Report.

  Junya Yamashita, Kazunobu Hashikawa, Yasumasa Kakei, Toshinori Sekitani, Masaya Akashi

  Implant-supported removable prostheses (ISrP) improve the quality of life, especially in patients who underwent mandibular reconstruction, but few studies have focused on the effect of ISrP in the fibular mandible on the function of the temporomandibular joint. The purpose of this pilot case series was to determine the usefulness of four-dimensional computed tomography (4DCT) images for the evaluation of differences in condylar movements with and without ISrP. Three patients who underwent ISrP following segmental mandibulectomy and free-flap reconstruction were evaluated. The participants were instructed to masticate a cookie during the 4DCT scan. The distance between the most anterior and posterior positions of the condyles on the sagittal view of the 4DCT images during the chewing of the cookies was measured and compared with and without ISrP. 4DCT revealed changes in the distances of condylar protrusion with and without wearing ISrP, but there were no obvious differences among the three patients. The 4DCT motion analysis was useful for the evaluation of the effect of wearing ISrP on condylar movements during mastication in patients with mandibular reconstruction and may become a useful objective evaluation method for the functional evaluation of ISrP.

  Corresponding, May 2023, Cureus, 15(5) (5), e39419, English, International magazine

  Link to Kobe Univ. Repository (Kernel)


• 歯科衛生士が口腔衛生管理を行った患者に対するCOVID-19発症状況について

  岡崎 葉菜, 武田 大介, 片山 めぐみ, 西井 美佳, 村木 友美, 長谷川 巧実, 明石 昌也

  (一社)日本口腔ケア学会, Apr. 2023, 日本口腔ケア学会雑誌, 17(3) (3), 172 - 172, Japanese


• COVID-19感染が契機と考えられたスティーブンスジョンソン症候群患者への口腔衛生管理の1例

  片山 めぐみ, 村木 友美, 岡崎 葉菜, 西井 美佳, 武田 大介, 長谷川 巧実, 明石 昌也

  (一社)日本口腔ケア学会, Apr. 2023, 日本口腔ケア学会雑誌, 17(3) (3), 229 - 229, Japanese


• Local application of a transcutaneous carbon dioxide paste prevents excessive scarring and promotes muscle regeneration in a bupivacaine-induced rat model of muscle injury

  Junya Hirota, Takumi Hasegawa, Atsuyuki Inui, Daisuke Takeda, Rika Amano-Iga, Nanae Yatagai, Izumi Saito, Satomi Arimoto, Masaya Akashi

  In postoperative patients with head and neck cancer, scar tissue formation may interfere with the healing process, resulting in incomplete functional recovery and a reduced quality of life. Percutaneous application of carbon dioxide (CO2) has been reported to improve hypoxia, stimulate angiogenesis, and promote fracture repair and muscle damage. However, gaseous CO2 cannot be applied to the head and neck regions. Previously, we developed a paste that holds non-gaseous CO2 in a carrier and can be administered transdermally. Here, we investigated whether this paste could prevent excessive scarring and promote muscle regeneration using a bupivacaine-induced rat model of muscle injury. Forty-eight Sprague Dawley rats were randomly assigned to either a control group or a CO2 group. Both groups underwent surgery to induce muscle injury, but the control group received no treatment, whereas the CO2 group received the CO2 paste daily after surgery. Then, samples of the experimental sites were taken on days 3, 7, 14, and 21 post-surgery to examine the following: (1) inflammatory (interleukin [IL]-1 beta, IL-6), and transforming growth factor (TGF)-beta and myogenic (MyoD and myogenin) gene expression by polymerase chain reaction, (2) muscle regeneration with haematoxylin and eosin staining, and (3) MyoD and myogenin protein expression using immunohistochemical staining. Rats in the CO2 group showed higher MyoD and myogenin expression and lower IL-1 beta, IL-6, and TGF-beta expression than the control rats. In addition, treated rats showed evidence of accelerated muscle regeneration. Our study demonstrated that the CO2 paste prevents excessive scarring and accelerates muscle regeneration. This action may be exerted through the induction of an artificial Bohr effect, which leads to the upregulation of MyoD and myogenin, and the downregulation of IL-1 beta, IL-6, and TGF-beta. The paste is inexpensive and non-invasive. Thus, it may be the treatment of choice for patients with muscle damage.

  WILEY, Apr. 2023, INTERNATIONAL WOUND JOURNAL, 20(4) (4), 1151 - 1159, English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Local application of a transcutaneous carbon dioxide paste prevents excessive scarring and promotes muscle regeneration in a bupivacaine-induced rat model of muscle injury.

  Junya Hirota, Takumi Hasegawa, Atsuyuki Inui, Daisuke Takeda, Rika Amano-Iga, Nanae Yatagai, Izumi Saito, Satomi Arimoto, Masaya Akashi

  In postoperative patients with head and neck cancer, scar tissue formation may interfere with the healing process, resulting in incomplete functional recovery and a reduced quality of life. Percutaneous application of carbon dioxide (CO2 ) has been reported to improve hypoxia, stimulate angiogenesis, and promote fracture repair and muscle damage. However, gaseous CO2 cannot be applied to the head and neck regions. Previously, we developed a paste that holds non-gaseous CO2 in a carrier and can be administered transdermally. Here, we investigated whether this paste could prevent excessive scarring and promote muscle regeneration using a bupivacaine-induced rat model of muscle injury. Forty-eight Sprague Dawley rats were randomly assigned to either a control group or a CO2 group. Both groups underwent surgery to induce muscle injury, but the control group received no treatment, whereas the CO2 group received the CO2 paste daily after surgery. Then, samples of the experimental sites were taken on days 3, 7, 14, and 21 post-surgery to examine the following: (1) inflammatory (interleukin [IL]-1β, IL-6), and transforming growth factor (TGF)-β and myogenic (MyoD and myogenin) gene expression by polymerase chain reaction, (2) muscle regeneration with haematoxylin and eosin staining, and (3) MyoD and myogenin protein expression using immunohistochemical staining. Rats in the CO2 group showed higher MyoD and myogenin expression and lower IL-1β, IL-6, and TGF-β expression than the control rats. In addition, treated rats showed evidence of accelerated muscle regeneration. Our study demonstrated that the CO2 paste prevents excessive scarring and accelerates muscle regeneration. This action may be exerted through the induction of an artificial Bohr effect, which leads to the upregulation of MyoD and myogenin, and the downregulation of IL-1β, IL-6, and TGF-β. The paste is inexpensive and non-invasive. Thus, it may be the treatment of choice for patients with muscle damage.

  Apr. 2023, International wound journal, 20(4) (4), 1151 - 1159, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Risk factors for pathological fracture in patients with mandibular osteoradionecrosis.

  Hiroaki Ohori, Eiji Iwata, Daisuke Takeda, Junya Kusumoto, Takumi Hasegawa, Masaya Akashi

  Osteoradionecrosis (ORN) often results in pathological fractures through progression. We aimed to identify the risk factors for pathological fracture in patients with mandibular ORN. Seventy-four patients with mandibular ORN were included in this retrospective study. We investigated various risk factors for pathological fracture in patients with mandibular ORN, including number of mandibular teeth with a poor prognosis each at initial evaluation before radiation therapy (RT) and when fracture occurred, and the proportion of antibiotic administration period in a follow-up duration after RT. The rate of occurrence of pathological fractures in patients with mandibular ORN was 25.7%. The median of duration between RT completion and fracture occurrence was 74.0 months. We found that pathological fracture was significantly associated with a larger number of mandibular teeth with a poor prognosis at initial evaluation before RT (P = 0.024) and when fracture occurred (P = 0.009). Especially, a larger number of mandibular teeth with P4 periodontitis, in other words severe periodontal status, was related to pathological fracture in both timings. The proportion of antibiotic administration period in a follow-up duration was also significant risk factor (P = 0.002). Multivariate analyses showed statistically significant associations between pathological fracture and a larger number of mandibular teeth with a poor prognosis when fracture occurred (hazard ratio 3.669). The patient with a larger number of mandibular teeth with P4 periodontitis may have a risk of not only occurrence of ORN but resulting in pathological fracture by accumulation of infection. Surgeons should consider extraction of those teeth regardless of before/after RT if necessary for infection control.

  Apr. 2023, Scientific reports, 13(1) (1), 5367 - 5367, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Multicenter retrospective study of the prognosis and the effect of postoperative adjuvant therapy in Japanese oral squamous cell carcinoma patients with close margin.

  Takumi Hasegawa, Yasumasa Kakei, Nobuhiro Yamakawa, Tadaaki Kirita, Masaya Okura, Tomofumi Naruse, Mitsunobu Otsuru, Shin-Ichi Yamada, Hiroshi Kurita, Eiji Hirai, Shin Rin, Michihiro Ueda, Masahiro Umeda, Masaya Akashi

  BACKGROUND: The purpose of this retrospective study was to investigate the prognosis of patients with oral cavity cancer with positive margin (PM) or close margin (CM) divided into pN- and pN+ groups. METHODS: The evaluated endpoints were local control and disease-specific survival (DSS) rates. RESULTS: Higher T classification, lymphovascular space invasion (LVSI), and older age were significant risk factors for DSS in the pN- groups. On the other hand, extranodal extension, multiple lymph node metastases, and LVSI were significant risk factors for DSS in the pN+ groups. Among the CM pN+ patients, no significant differences in the 3-year DSS were observed between the only surgery (51.9%) and adjuvant groups (53.2%). CONCLUSIONS: Higher T classification and LVSI are high-risk features more than PM or CM in the pN- groups for DSS. However, further prospective studies are needed to demonstrate the usefulness of adjuvant treatment in patients with PM or CM.

  Mar. 2023, Head & neck, 45(6) (6), 1418 - 1429, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Efficacy and Long-Term Safety of Ibuprofen Gargle for Oral Lichen Planus: A Study Protocol of Randomized Crossover and Long-Term Extension Trials

  Yumi Kitahiro, Takeshi Ioroi, Yasumasa Kakei, Junya Yamashita, Akira Kimoto, Takumi Hasegawa, Asami Morioka, Kazuhiro Yamamoto, Masaya Akashi, Ikuko Yano

  Oral lichen planus (OLP) is a type of chronic and refractory stomatitis characterized by abnormal keratinization, which is often painful. There is no consensus regarding treatment options for OLP, particularly in the presence of pain. The current study protocol focuses on the short-term efficacy and long-term safety of an ibuprofen gargle for pain management in patients with OLP. Patients (n = 24) with painful OLP will be enrolled. During a crossover study period, patients in the ibuprofen–placebo (IP) group will receive an ibuprofen gargle (0.6%) on day 1, a placebo gargle on day 2, and an ibuprofen gargle on days 3–5 at least once daily. Patients in the placebo–ibuprofen (PI) group will receive a placebo gargle on day 1, an ibuprofen gargle on day 2, and an ibuprofen gargle on days 3–5 at least once daily. The primary endpoint of the crossover study period is the change in pain level as measured by a visual analogue scale score from before gargle administration to 5 min after gargle administration on days 1 and 2. The primary endpoint of the long-term extension study is assessment of long-term safety. The results of this study may support existing evidence regarding the effectiveness of ibuprofen rinses in treating OLP.

  MDPI AG, Jan. 2023, Methods and Protocols, 6(1) (1), 7 - 7

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Transcutaneous carbon dioxide application suppresses the expression of cancer-associated fibroblasts markers in oral squamous cell carcinoma xenograft mouse model.

  Yoshiaki Tadokoro, Daisuke Takeda, Aki Murakami, Nanae Yatagai, Izumi Saito, Satomi Arimoto, Yasumasa Kakei, Masaya Akashi, Takumi Hasegawa

  Oral squamous cell carcinoma (OSCC) is the most common head and neck cancer. Cancer-associated fibroblasts (CAFs) are the main stromal cells in the tumor microenvironment (TME). As CAFs promote tumor progression and hypoxia in the TME, regulating the conversion of normal fibroblasts (NFs) into CAFs is essential for improving the prognosis of patients with OSCC. We have previously reported the antitumor effects of transcutaneous carbon dioxide (CO2) application in OSCC. However, the effects of reducing hypoxia in the TME remain unclear. In this study, we investigated whether CO2 administration improves the TME by evaluating CAFs marker expression. Human OSCC cells (HSC-3) and normal human dermal fibroblasts (NHDF) were coinjected subcutaneously into the dorsal region of mice. CO2 gas was applied twice a week for 3 weeks. The tumors were harvested six times after transcutaneous CO2 application. The expression of CAFs markers (α-SMA, FAP, PDPN, and TGF-β) were evaluated by using real-time polymerase chain reaction and immunohistochemical staining. The expression of α-SMA, FAP, PDPN, and TGF-β was significantly increased over time after co-injection. In the CO2-treated group, tumor growth was significantly suppressed after treatment initiation. In addition, the mRNA expression of these markers was significantly inhibited. Furthermore, immunohistochemical staining revealed a significant decrease in the protein expression of all CAFs markers in the CO2-treated group. We confirmed that transcutaneous CO2 application suppressed CAFs marker expression and tumor growth in OSCC xenograft mouse model.

  2023, PloS one, 18(8) (8), e0290357, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Relationship between oral health and prognosis in patients with empyema: Single center retrospective study with propensity score matching analysis.

  Eiji Iwata, Teruaki Nishiuma, Suya Hori, Keiko Sugiura, Masato Taki, Shuntaro Tokunaga, Junya Kusumoto, Takumi Hasegawa, Akira Tachibana, Masaya Akashi

  BACKGROUND: Empyema is a life-threatening infection often caused by oral microbiota. To the best of our knowledge, no reports have investigated the association between the objective assessment of oral health and prognosis in patients with empyema. MATERIALS AND METHODS: A total of 63 patients with empyema who required hospitalization at a single institution were included in this retrospective study. We compared non-survivors and survivors to assess risk factors for death at three months, including the Renal, age, pus, infection, diet (RAPID) score, and Oral Health Assessment Tool (OHAT) score. Furthermore, to minimize the background bias of the OHAT high-score and low-score groups determined based on the cut-off value, we also analyzed the association between the OHAT score and death at 3 months using the propensity score matching method. RESULTS: The 3-month mortality rate was 20.6% (13 patients). Multivariate analysis showed that a RAPID score ≥5 points (odds ratio (OR) 8.74) and an OHAT score ≥7 points (OR 13.91) were significantly associated with death at 3 months. In the propensity score analysis, a significant association was found between a high OHAT score (≥7 points) and death at 3 months (P = 0.019). CONCLUSION: Our results indicated that oral health assessed using the OHAT score may be a potential independent prognostic factor in patients with empyema. Similar to the RAPID score, the OHAT score may become an important indicator for the treatment of empyema.

  2023, PloS one, 18(3) (3), e0282191, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Hematologic and inflammatory parameters for determining severity of odontogenic infections at admission: a retrospective study.

  Junya Kusumoto, Eiji Iwata, Wensu Huang, Naoki Takata, Akira Tachibana, Masaya Akashi

  BACKGROUND: Severe odontogenic infections in the head and neck region, especially necrotizing soft tissue infection (NSTI) and deep neck abscess, are potentially fatal due to their delayed diagnosis and treatment. Clinically, it is often difficult to distinguish NSTI and deep neck abscess in its early stage from cellulitis, and the decision to perform contrast-enhanced computed tomography imaging for detection is often a challenge. This retrospective case-control study aimed to examine the utility of routine blood tests as an adjunctive diagnostic tool for NSTI in the head and neck region and deep neck abscesses. METHODS: Patients with severe odontogenic infections in the head and neck region that required hospitalization were classified into four groups. At admission, hematologic and inflammatory parameters were calculated according to the blood test results. In addition, a decision tree analysis was performed to detect NSTI and deep neck abscesses. RESULTS: There were 271 patients, 45.4% in Group I (cellulitis), 22.5% in Group II (cellulitis with shallow abscess formation), 27.3% in Group III (deep neck abscess), and 4.8% in Group IV (NSTI). All hematologic and inflammatory parameters were higher in Groups III and IV. The Laboratory Risk Indicator for Necrotizing Fasciitis score, with a cut-off value of 6 and C-reactive protein (CRP) + the neutrophil-to-lymphocyte ratio (NLR), with a cut-off of 27, were remarkably useful for the exclusion diagnosis for Group IV. The decision tree analysis showed that the systemic immune-inflammation index (SII) of ≥ 282 or < 282 but with a CRP + NLR of ≥ 25 suggests Group III + IV and the classification accuracy was 89.3%. CONCLUSIONS: Hematologic and inflammatory parameters calculated using routine blood tests can be helpful as an adjunctive diagnostic tool in the early diagnosis of potentially fatal odontogenic infections. An SII of ≥ 282 or < 282 but with a CRP + NLR of ≥ 25 can be useful in the decision-making for performing contrast-enhanced computed tomography imaging.

  Dec. 2022, BMC infectious diseases, 22(1) (1), 931 - 931, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Perioperative bridging anticoagulation in patients with oral cancer surgery: a multicenter retrospective study

  Ryo Kajihara, Shin ichi Yamada, Takumi Hasegawa, Hitoshi Yoshimura, Hidetake Tachinami, Kei Tomihara, On Hasegawa, Sumiyo Hishida, Nobuhiro Ueda, Kenjiro Okamoto, Sakiko Soutome, Yuya Denda, Masaaki Miyakoshi, Kiriko Matsuzawa, Eiji Kondo, Yoshimasa Kitagawa, Daichi Chikazu, Yoshihide Ota, Yasuyuki Shibuya, Masaya Akashi, Tatsushi Matsumura, Masahiro Umeda, Tadaaki Kirita, Makoto Noguchi, Hiroshi Kurita

  The number of patients administered oral antithrombotic drugs who require elective surgery has increased. Perioperative heparin bridging needs to be considered for patients at a high risk of thromboembolism. The present study investigated the effects of perioperative heparin bridging therapy on perioperative complications (postoperative bleeding and thrombotic events) in oral cancer surgery. The medical records of 98 patients who underwent oral cancer surgery with perioperative bridging therapy were retrospectively analyzed. Postoperative hemorrhage occurred in 16 patients (16.3%) and thrombotic events (deep venous thrombosis) in 5 (5.1%), which were higher than in other surgeries. A multivariate analysis identified the administration of prostaglandins as the only significant independent risk factor for postoperative hemorrhage, and a longer duration of postoperative heparin bridging therapy was associated with the incidence of deep venous thrombosis. The present results suggest a higher incidence of thrombotic and bleeding events in patients who underwent oral cancer surgery with heparin bridging, and the nature of surgery (such as revascularization and prolonged bed rest) may be a contributing factor. Future studies are needed to establish whether heparin bridging is effective in oral cancer surgery.

  Nov. 2022, Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, 34(6) (6), 734 - 739


• 当科における口腔癌患者の広範囲顎骨支持型装置の成功率に関する後ろ向き観察研究

  早瀬 悠夏, 長谷川 巧実, 白井 達也, 平岡 佑二郎, 有本 智美, 武田 大介, 筧 康正, 木本 明, 明石 昌也

  (公社)日本顎顔面インプラント学会, Nov. 2022, Japanese Journal of Maxillo Facial Implants, 21(3) (3), 237 - 237, Japanese


• The Impact of Pretreatment Low Body Mass Index on Cause-Specific Mortality in Patients with Squamous Cell Carcinoma of the Oral Cavity.

  Kyoko Kurioka, Shin Rin, Mitsunobu Otsuru, Tomohumi Naruse, Takumi Hasegawa, Nobuhiro Yamakawa, Shin-Ichi Yamada, Eiji Hirai, Kozo Yamamoto, Michihiro Ueda, Tadaaki Kirita, Masaya Akashi, Hiroshi Kurita, Yoichi Ohiro, Masaya Okura

  The association between the pretreatment body mass index (BMI) and oral squamous cell carcinoma (SCC) outcomes is controversial. We aimed to examine the association between BMI and cause-specific mortality due to cancer of the oral cavity and patterns of failure that correlate with increased mortality. We enrolled 2,023 East Asian patients in this multicenter cohort study. We used the cumulative incidence competing risks method and the Fine-Gray model to analyze factors associated with cause-specific mortality, local recurrence, regional metastasis, and distant metastasis as first events. The median follow-up period was 62 mo. The 5-year cause-specific mortality for patients with underweight was 25.7%, which was significantly higher than that for patients with normal weight (12.7%, P < 0.0001). The multivariate model revealed that underweight was an independent risk factor for cause-specific mortality and regional metastasis (P < 0.05). Moreover, patients with underweight displayed a 51% and 55% increased risk of cause-specific mortality and regional metastasis, respectively, compared with their normal weight counterparts. Local recurrence was not associated with the BMI categories; however, the incidence of distant metastasis inversely decreased with BMI value. In summary, being underweight at diagnosis should be considered a high-risk mortality factor for oral SCC.

  Oct. 2022, Nutrition and cancer, 1 - 12, English, International magazine

  Scientific journal


• Prevalence of and risk factors for postoperative complications after lower third molar extraction: A multicenter prospective observational study in Japan.

  Shin-Ichi Yamada, Takumi Hasegawa, Nobuhiko Yoshimura, Yusuke Hakoyama, Tetsuya Nitta, Narihiro Hirahara, Hironori Miyamoto, Hitoshi Yoshimura, Nobuhiro Ueda, Yoshiko Yamamura, Hideki Okuyama, Atsushi Takizawa, Yoshitaka Nakanishi, Eiji Iwata, Daisuke Akita, Ryuichi Itoh, Kiriko Kubo, Seiji Kondo, Hironobu Hata, Yoshito Koyama, Youji Miyamoto, Hirokazu Nakahara, Masaya Akashi, Tadaaki Kirita, Yasuyuki Shibuya, Masahiro Umeda, Hiroshi Kurita

  Lower third molar extraction is the most common surgical treatment among routine dental and oral surgical procedures. while the surgical procedures for lower third molar extraction are well established, the difficulty of tooth extraction and the frequency of postoperative complications differ depending on the patient's background. To establish a management protocol for the lower third molars, the prevalence of and risk factors for postoperative complications after lower third molar extraction were investigated in a large number of Japanese patients in a multicenter prospective study. During 6 consecutive months in 2020, 1826 lower third molar extractions were performed at the 20 participating institutions. The medical records of the patients were reviewed, and relevant data were extracted. The prevalence of and risk factors for postoperative complications were analyzed. The prevalence of postoperative complications after lower third molar extraction was 10.0%. Multivariate analysis indicated that age (≤32 vs >32, odds ratio [OR]: 1.428, 95% confidence interval [95% CI]: 1.040-1.962, P < .05), the radiographic anatomical relationship between the tooth roots and mandibular canal (overlapping of the roots and canal vs no close anatomical relationship between the roots and the superior border of the canal, OR: 2.078, 95% CI: 1.333-3.238, P < .01; overlapping of the roots and canal vs roots impinging on the superior border of the canal, OR: 1.599, 95% CI: 1.050-2.435, P < .05), and impaction depth according to the Pell and Gregory classification (position C vs position A, OR: 3.7622, 95% CI: 2.079-6.310, P < .001; position C vs position B, OR: 2.574, 95% CI: 1.574-4.210, P < .001) are significant independent risk factors for postoperative complications after lower third molar extraction. These results suggested that higher age and a deeply impacted tooth might be significant independent risk factors for postoperative complications after lower third molar extraction.

  Aug. 2022, Medicine, 101(32) (32), e29989, English, International magazine

  Scientific journal


• Actinomycosis of the Lower Lip: Report of a Case

  Manabu Shigeoka, Daisuke Takeda, Masaya Akashi

  Actinomycosis is usually a chronic infectious disease caused by Actinomyces species, which are anaerobic Gram-positive bacteria that normally colonize the human oral cavity and digestive and urogenital tracts. Although this lesion often occurs on soft tissue around the mandible, cases localized in the lip are very uncommon. We encountered a patient with actinomycosis in the lower lip. A 76-year-old woman with a 5-mm submucosal nodule of the lower lip was referred to our hospital from a dental clinic. The clinical diagnosis was a benign submucosal tumor. Total excision and histological examination were conducted. No oral antibiotic therapy was prescribed. The histological diagnosis was actinomycosis. The postoperative course was uneventful with no signs of recurrence during the 6 months after surgery.

  HINDAWI LTD, Aug. 2022, CASE REPORTS IN DENTISTRY, 2022, English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Treatment and Prognosis of Oral Cancer Patients with Confirmed Contralateral Neck Metastasis: A Multicenter Retrospective Analysis.

  Yuki Sakamoto, Mitsunobu Otsuru, Takumi Hasegawa, Masaya Akashi, Shin-Ichi Yamada, Hiroshi Kurita, Masaya Okura, Nobuhiro Yamakawa, Tadaaki Kirita, Souichi Yanamoto, Masahiro Umeda, Yuka Kojima

  The prognosis of oral cancer that has metastasized to the contralateral cervical lymph nodes is poor, although the appropriate treatment method has not been established because of its rarity. A multicenter retrospective study on the treatment and prognosis of pN2c oral cancer patients was conducted. We investigated the treatment and prognosis of 62 pN2c patients out of 388 pN+ patients with oral squamous cell carcinomas. Statistical analysis was performed on the various factors with overall survival (OS) and disease specific survival (DSS). In multivariate cox regression analysis, advanced T stage was significantly correlated with poor OS (p = 0.011) and DSS (p = 0.023) of patients with pN2c neck. In pN2c patients, OS, DSS, and neck control was not different between those undergoing ipsilateral neck dissection initially and those undergoing bilateral neck dissection. Thus, contralateral elective neck dissection is not recommended. The most important risk factor for prognosis in pN2c oral cancer patients is advanced T stage. No evidence was found to recommend contralateral elective neck dissection in clinically N1/2b patients. Therefore, the indication for contralateral elective neck dissection in N1/2b patients should be carefully determined in consideration of individual conditions.

  Jul. 2022, International journal of environmental research and public health, 19(15) (15), English, International magazine

  Scientific journal


• Exploratory clinical trial to evaluate the efficacy and safety of carbon dioxide paste in healthy people.

  Nanae Yatagai, Takumi Hasegawa, Katsusuke Kyotani, Tomohiro Noda, Rika Amano, Izumi Saito, Satomi Arimoto, Daisuke Takeda, Yasumasa Kakei, Masaya Akashi

  INTRODUCTION: Scarring and pain are postoperative complications in patients after head and neck cancer treatment; however, there is no effective treatment. These complications are affected by local blood flow disorders, and it is well known that the transcutaneous application of carbon dioxide (CO2) improves local blood flow. Previously, we have shown that the transcutaneous application of carbon dioxide causes absorption of CO2 and increase the oxygen (O2) pressure in the treated tissue; it is expected that the application of CO2 may reduce scarring and pain caused by cancer treatment. We newly introduced the CO2 paste as a new CO2 application method, which does not need to use CO2 gas directly. In this study, we aimed to apply of CO2 paste to healthy people and to investigate its usefulness, safety and feasibility by analysing the increase in blood flow and frequency of adverse events. METHODS: We applied carbon dioxide paste to skin over the sternocleidomastoid and gastrocnemius muscles of eight healthy volunteers. The changes in blood flow before and after the CO2 paste application using dynamic MRI, and changes in the vital signs were evaluated. RESULTS: In the neck area and middle layer of the lower leg, the signal intensity (SI) significantly increased 60 seconds after application. In the surface layer of the lower leg, the SI was significantly increased 60 and 300 seconds after paste application. Although mild heat was noted after the paste application, no obvious adverse events occurred. CONCLUSION: We demonstrated the increase in SI by dynamic MRI at the site of the carbon dioxide paste application, which indicates the paste application is effective in improving the blood flow.

  Jul. 2022, Medicine, 101(29) (29), e29511, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Cover Image

  Junya Kusumoto, Kazunobu Hashikawa, Akiko Sakakibara, Nobuyuki Murai, Masaya Akashi

  Wiley, Jul. 2022, Microsurgery, 42(5) (5)

  Scientific journal


• 当科における下顎骨びまん性硬化性骨髄炎症例に関する臨床的検討

  早瀬 悠夏, 楠元 順哉, 古土井 春吾, 武田 大介, 明石 昌也

  (NPO)日本口腔科学会, Jul. 2022, 日本口腔科学会雑誌, 71(2) (2), 81 - 81, Japanese


• 放射線性顎骨壊死患者を対象とした予後とリスク因子に関する後ろ向き観察研究

  田所 慶誠, 長谷川 巧実, 佐藤 匠, 平岡 佑二郎, 有本 智美, 武田 大介, 楠元 順哉, 明石 昌也

  (NPO)日本口腔科学会, Jul. 2022, 日本口腔科学会雑誌, 71(2) (2), 137 - 137, Japanese


• 緩和照射に関する臨床的検討

  松添 雄大, 南川 勉, 瓜生 開人, 棚倉 万紀子, 筧 康正, 長谷川 巧実, 明石 昌也

  (NPO)日本口腔科学会, Jul. 2022, 日本口腔科学会雑誌, 71(2) (2), 91 - 91, Japanese


• 局所的炭酸ガス投与による口腔扁平上皮癌の腫瘍免疫抑制に対する改善効果

  八谷 奈苗, 長谷川 巧実, 武田 大介, 筧 康正, 榊原 晶子, 明石 昌也

  (NPO)日本口腔科学会, Jul. 2022, 日本口腔科学会雑誌, 71(2) (2), 110 - 110, Japanese


• Evaluation of the Effects of Covering With Polyglycolic Acid Sheet on Wound Healing: A Pilot Histopathological Study.

  Yasumasa Kakei, Kazunobu Hashikawa, Kaito Uryu, Ryuichiro Funahara, Manabu Shigeoka, Masaya Akashi

  PURPOSE: Although the usefulness of polyglycolic acid (PGA) sheet for wound dressing has been recently reported, its histopathological effect on wound healing is not completely elucidated. This pilot study focused on the neo-epithelium formation and the remaining inflammation. METHODS: Full-thickness defects of 8 mm were created on the back of seven-week-old rats. Four rats were divided into the control (raw surface) group and the PGA group, in which the wounds were covered with a PGA sheet. The wounds were assessed on days seven and 12 after wound creation. The length of neo-epithelium on day seven was measured by referring to Masson's trichrome (MT) and α-smooth muscle actin (α-SMA) staining. The remaining inflammation on days seven and 12 was assessed with ionized calcium-binding adapter molecule 1 (Iba-1) staining. RESULTS: The average values of neo-epithelium length on day seven measured by referring to the borderline between MT staining and α-SMA expression were 959.2 μm in the control group and 582.2 μm in the PGA group. The number of Iba-1-positive cells on day 12 was significantly higher in the PGA group than in the control group. CONCLUSIONS: To assess the neo-epithelium length and the remaining inflammation, the α-SMA, MT, and Iba-1 staining may be appropriate.

  Jul. 2022, Cureus, 14(7) (7), e27209, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effect of compression on mandibular fracture haematoma-derived cells.

  Satomi Arimoto, Takumi Hasegawa, Eiji Iwata, Daisuke Takeda, Masaya Akashi

  Mechanical stress induces a variety of biochemical and morphological reactions in bone cell biology. This study aimed to investigate appropriate pressures of osteogenesis on the biological responses of 3-dimensional cultured human mandibular fracture haematoma-derived cells by compressive loading. Six patients with mandibular fractures who underwent open reduction and internal fixation were included in the study. During the operation, fracture haematomas that formed fibrin clots were manually removed before irrigation. First, pressures were applied to human mandibular fracture haematoma-derived cell-seeded collagen sponges. The sponges were subjected to mechanical compression using loading equipment applied at no compression, 0.5, or 1 mm. Compressive loading was applied to the samples prior to compression for 0, 6, 12, or 24 hours. Collagen sponge samples were collected for quantification of mRNA using several parameters including alkaline phosphatase (ALP), osteopontin (OPN), osterix (OSX), runt-related gene 2 (RUNX2), protein level, and immunocytochemistry (anti-sclerostin). Among these the 0.5 mm compression group compared with the control and 1.0 mm compression groups upregulated mRNA expression of OPN and OSX after 24 hours. Additionally, compared with the control group, a significantly higher OSX gene expression was observed in both the 0.5 mm and 1.0 mm groups after 6, 12, and 24 hours of compression (p < 0.05). However, no significant differences were observed regarding ALP and RUNX2 expression. These results indicated increased stimulation of osteogenesis of the mandibular fracture-line gap in the 0.5 mm compression group compared with the control and 1.0 mm compression groups.

  Jun. 2022, The British journal of oral & maxillofacial surgery, 60(9) (9), 1216 - 1223, English, International magazine

  Scientific journal


• 放射線性下顎骨壊死の低体重患者に下顎骨区域切除を行った1例

  早瀬 悠夏, 楠元 順哉, 薄田 友理子, 佐藤 匠, 印南 勇祐, 明石 昌也

  (一社)日本口腔感染症学会, Jun. 2022, 日本口腔感染症学会雑誌, 29(1) (1), 34 - 35, Japanese


• Factors Associated with Treatment Outcomes and Pathological Features in Patients with Osteoradionecrosis: A Retrospective Study

  Yoshiaki Tadokoro, Takumi Hasegawa, Daisuke Takeda, Aki Murakami, Nanae Yatagai, Eiji Iwata, Izumi Saito, Junya Kusumoto, Masaya Akashi

  A standard treatment for osteoradionecrosis (ORN) has not yet been established because of the diversity. Therefore, identifying the risk factors for a poor prognosis is essential. This study retrospectively investigated the factors associated with the prognosis of ORN in 68 patients. Relevant clinical data of all patients were obtained. Of the patients, 16 who underwent extensive surgery underwent histopathological analysis. The necrotic changes of the anterior and posterior margins in the cortical and cancellous bones were investigated. Multivariate analyses showed statistically significant associations between poor prognosis in patients with ORN and high radiation dose (hazard ratio [HR] 1.15), orocutaneous fistula (HR 2.93), and absence of sequestration (HR 2.49). Histopathological analysis showed a viable anterior margin of the middle portion of the cortical bone for all recovered cases; in contrast, most cases (75%) with a poor prognosis showed necrotic changes. The anterior margin of the cancellous bone was viable and resilient to high irradiation, regardless of the prognosis. These results suggest that patients with orocutaneous fistula should receive early surgical intervention, even if the affected area is limited or asymptomatic. In extensive surgery, a sufficient safety margin of necrotic bone, particularly in the anterior region, is required to improve the prognosis.

  MDPI, Jun. 2022, INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 19(11) (11), English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Blowing time ratio and high-resolution manometry to evaluate swallowing function of patients with oral and oropharyngeal cancer.

  Hirokazu Komatsu, Tatsuya Furukawa, Keisuke Iritani, Shun Tatehara, Miki Takahashi, Shinobu Iwaki, Yasumasa Kakei, Takumi Hasegawa, Masanori Teshima, Hirotaka Shinomiya, Naoki Otsuki, Kazunobu Hashikawa, Naomi Kiyota, Ryohei Sasaki, Masaya Akashi, Ken-Ichi Nibu

  OBJECTIVE: The blowing time ratio, which is the ratio of the blowing time when the nostrils are open and closed, is significantly correlated with velopharyngeal pressure, not only during speech but also during swallowing. This study aimed to further evaluate the usefulness of the blowing time ratio as a screening tool to evaluate the swallowing pressure of patients treated for oral and oropharyngeal cancers using high-resolution manometery (HRM). METHODS: Ten patients treated for oral or oropharyngeal cancer were recruited for this study. Swallowing pressures at the velopharynx, oropharynx, and upper esophageal sphincter (UES) were measured using HRM. Their correlations with the blowing time ratio were analyzed. RESULTS: The blowing time ratio was significantly correlated with the swallowing pressures of the oropharynx (CC = 0.815, p = 0.004) and the velopharynx (CC = 0.657, p = 0.039), but not of the UES. CONCLUSIONS: The present results further support our previous finding that the blowing time ratio is a useful screening tool to evaluate velopharyngeal and oropharyngeal swallowing pressures in patients treated for oral and oropharyngeal cancer.

  Jun. 2022, Auris, nasus, larynx, 49(3) (3), 477 - 483, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Factors Associated with Treatment Outcomes and Pathological Features in Patients with Osteoradionecrosis: A Retrospective Study.

  Yoshiaki Tadokoro, Takumi Hasegawa, Daisuke Takeda, Aki Murakami, Nanae Yatagai, Eiji Iwata, Izumi Saito, Junya Kusumoto, Masaya Akashi

  A standard treatment for osteoradionecrosis (ORN) has not yet been established because of the diversity. Therefore, identifying the risk factors for a poor prognosis is essential. This study retrospectively investigated the factors associated with the prognosis of ORN in 68 patients. Relevant clinical data of all patients were obtained. Of the patients, 16 who underwent extensive surgery underwent histopathological analysis. The necrotic changes of the anterior and posterior margins in the cortical and cancellous bones were investigated. Multivariate analyses showed statistically significant associations between poor prognosis in patients with ORN and high radiation dose (hazard ratio [HR] 1.15), orocutaneous fistula (HR 2.93), and absence of sequestration (HR 2.49). Histopathological analysis showed a viable anterior margin of the middle portion of the cortical bone for all recovered cases; in contrast, most cases (75%) with a poor prognosis showed necrotic changes. The anterior margin of the cancellous bone was viable and resilient to high irradiation, regardless of the prognosis. These results suggest that patients with orocutaneous fistula should receive early surgical intervention, even if the affected area is limited or asymptomatic. In extensive surgery, a sufficient safety margin of necrotic bone, particularly in the anterior region, is required to improve the prognosis.

  May 2022, International journal of environmental research and public health, 19(11) (11), English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Efficacy of Ibuprofen Gargle for Postoperative Pain After Mandibular Third Molar Extraction: Protocol for a Phase II, Placebo-Controlled, Double-Blind, Randomized Crossover Trial.

  Yasumasa Kakei, Takeshi Ioroi, Takahiro Ito, Yutaro Okazaki, Takumi Hasegawa, Ikuko Yano, Masaya Akashi

  BACKGROUND: Extraction of mandibular third molars is one of the most commonly performed oral surgical procedures, and nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used for pain management. Oral NSAIDs are associated with adverse events such as gastrointestinal disorders, renal and hepatic dysfunction, and platelet dysfunction. Topical analgesics have been proposed as alternatives to oral and injectable medications to safely improve postoperative pain relief. We will conduct a single-center, placebo-controlled, double-blind, randomized crossover trial to assess the pain-relieving effect of an ibuprofen-containing gargle in patients undergoing extraction of mandibular third molars when compared with a placebo gargle. OBJECTIVE: This will be the first clinical study to compare the efficacy of an ibuprofen gargle with that of a placebo for relieving postoperative pain in addition to loxoprofen after mandibular third molar extraction. METHODS: This study will be performed at Kobe University Hospital. Participants (N=40) will be randomized equally to 1 of 2 groups. The ibuprofen-placebo group will receive an ibuprofen gargle on postoperative day (POD) 1 and a placebo gargle on POD 2. The placebo-ibuprofen group will receive a placebo gargle on POD 1 and an ibuprofen gargle on POD 2. Both groups will receive ibuprofen gargles on PODs 3-5 at least once daily. The primary objective is to estimate the within-subject difference on a visual analog scale (VAS) before and 5 minutes after using the ibuprofen or placebo gargle on PODs 1 and 2. The secondary objectives are to estimate the within-subject differences in ΔVAS before and 15 minutes after using the ibuprofen or placebo gargle on PODs 1 and 2, ΔVAS before and 5 or 15 minutes after using the ibuprofen gargle on PODs 3-5, overall efficacy (self-completion, 5 scales) on PODs 1-5, daily frequency of use (ibuprofen or placebo gargle and analgesics) on PODs 1-7, and the occurrence of adverse events. RESULTS: The Certified Review Board of Kobe University approved the study. The intervention was implemented in May 2021. For the primary analysis, we will calculate the mean and SD of ΔVAS5 on PODs 1 and 2 and the within-study difference in ΔVAS5. The treatment effect will be estimated by dividing the mean ΔVAS5 in the within-subject difference by 2 and calculating the P value using an unpaired t test. For the secondary analysis, we will calculate the mean and SD of ΔVAS15 on PODs 1 and 2 and the within-study difference in ΔVAS15. The treatment effect will be estimated as in the primary analysis. CONCLUSIONS: This trial will provide exploratory evidence of the efficacy and safety of an ibuprofen gargle for pain reduction after mandibular third molar extraction. TRIAL REGISTRATION: Japan Registry of Clinical Trials jRCTs051210022; https://tinyurl.com/39ej23zu. INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID): DERR1-10.2196/35533.

  May 2022, JMIR research protocols, 11(5) (5), e35533, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Significance of intratumoural CD163+ macrophages in oral malignant melanoma: A preliminary study.

  Takayuki Kodama, Manabu Shigeoka, Mari Nishio, Yu-Ichiro Koma, Masaya Akashi, Hiroshi Yokozaki

  May 2022, Oral diseases, English, International magazine

  Scientific journal


• Perioperative oral care can prevent surgical site infection after colorectal cancer surgery: A multicenter, retrospective study of 1,926 cases analyzed by propensity score matching.

  Hiroshi Nobuhara, Yasuhiro Matsugu, Sakiko Soutome, Saki Hayashida, Takumi Hasegawa, Masaya Akashi, Shin-Ichi Yamada, Hiroshi Kurita, Hirokazu Nakahara, Miyuka Nakahara, Nobuhiro Ueda, Tadaaki Kirita, Tomohisa Nakamura, Yasuyuki Shibuya, Kazuyo Mori, Taihei Yamaguchi

  BACKGROUND: Surgical site infection is a common postoperative complication of colorectal cancer surgery, and surgical site infection increases medical costs, prolongs hospitalization, and worsens long-term prognosis. Perioperative oral care has been reported to be effective in preventing postoperative pneumonia, although there are only a few reports on its effectiveness in preventing surgical site infection. This study aimed to determine the role of perioperative oral care in surgical site infection prevention after colorectal cancer surgery. METHODS: In this study, 1,926 patients with colorectal cancer from 8 institutions were enrolled; 808 patients (oral care group) received perioperative oral care at the hospital's dental clinic, and 1,118 (control group) did not receive perioperative oral care. The data were matched by propensity score to reduce bias. Ultimately, a total of 1,480 patients were included in the analysis. RESULTS: The incidence of surgical site infection was significantly lower in the oral care group than in the control group (8.4% vs 15.7%, P < .001). Multivariate logistic regression analysis revealed 4 independent risk factors for surgical site infection: low albumin level, rectal cancer, blood loss, and lack of perioperative oral care. Lack of perioperative oral care had an odds ratio of 2.100 (95% confidence interval 1.510-2.930, P < .001). CONCLUSION: These results suggest that perioperative oral care can reduce the incidence of surgical site infection after colorectal cancer resection. Perioperative oral care may have an important role in the future perioperative management of colorectal cancer as a safe and effective method of surgical site infection prevention, although further validation in prospective studies is needed.

  Apr. 2022, Surgery, 172(2) (2), 530 - 536, English, International magazine

  Scientific journal


• 口腔粘膜炎に対するエピシル口腔溶液の効果や有効性について

  岡崎 葉菜, 武田 大介, 西井 美佳, 村川 智美, 片山 めぐみ, 澤田 麻衣子, 楠元 順哉, 明石 昌也

  (一社)日本口腔ケア学会, Apr. 2022, 日本口腔ケア学会雑誌, 16(3) (3), 140 - 140, Japanese


• 頭頸部がん放射線療法における口腔カンジダ症に対するミコナゾール貼付薬の有効性について

  西井 美佳, 楠元 順哉, 村川 智美, 岡崎 葉菜, 片山 めぐみ, 澤田 麻衣子, 武田 大介, 明石 昌也

  (一社)日本口腔ケア学会, Apr. 2022, 日本口腔ケア学会雑誌, 16(3) (3), 141 - 141, Japanese


• Strategy for preventing skin paddle necrosis in mandibular reconstruction with free fibula osteocutaneous flap.

  Junya Kusumoto, Kazunobu Hashikawa, Akiko Sakakibara, Nobuyuki Murai, Masaya Akashi

  BACKGROUND: Non-thrombotic skin paddle necrosis occasionally occurs during mandibular reconstructions with free fibula osteocutaneous flaps. The number of perforators, size of the skin paddle, and ischemia time of the flap are considered as causes of skin paddle necrosis. The importance of donor side selection has also been highlighted. This study aimed to investigate the leading cause of skin paddle necrosis and the optimal reconstructive procedure. METHODS: A total of 66 patients who underwent mandibular reconstruction using a free fibula osteocutaneous flap were retrospectively analyzed. Skin paddle necrosis, number of cutaneous perforators, size of the skin paddle, and ischemia time of the flap were investigated. An incorrect "laterality" was defined as a skin paddle (septum) covering the reconstruction plate. Donor-site morbidity was recorded. RESULTS: Skin paddle necrosis occurred in 15.2% of patients. An incorrect laterality was associated with a higher incidence of skin paddle necrosis (odds ratio, 22.0; 95% confidence interval, 2.5-195; p = .005). Donor-site morbidity was noted in 18.8% of the patients, without any significant difference in terms of the donor side with and without skin graft (p = .592). The postoperative activities of daily living were not affected. CONCLUSIONS: To prevent skin paddle necrosis, donor side selection is an important safety strategy during mandibular reconstruction with free fibula osteocutaneous flap. The postoperative activities of daily living were found to be little affected by differences in the donor side.

  Mar. 2022, Microsurgery, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Success of dental implants in patients with large bone defect and analysis of risk factors for implant failure: a non-randomized retrospective cohort study.

  Takumi Hasegawa, Aki Sasaki, Izumi Saito, Satomi Arimoto, Nanae Yatagai, Yujiro Hiraoka, Daisuke Takeda, Yasumasa Kakei, Masaya Akashi

  OBJECTIVES: This study aimed to retrospectively investigate the success and survival rates of dental implants used for dentomaxillary prostheses at our hospital and the risk factors associated with large bone defects. MATERIALS AND METHODS: A total of 138 external joint system implants used for dentomaxillary prostheses in 40 patients with large bone defects were included in this study. The alveolar bone at the site of implant insertion was evaluated using panoramic radiography and computed tomography. Various risk factors (demographic characteristics, dental status, and operative factors such as the employment of alveolar bone augmentation, the site, the length, and diameter of implants) for implant failure and complete implant loss were investigated using univariate and multivariate analyses. The associations between the variables and the success and survival rates of dental implants were analyzed using the multivariate Cox proportional hazard models. RESULTS: The 10-year overall success and survival rates were 81.3% and 88.4% in this study. Multivariable analysis showed that the male sex (HR 6.22), shorter implants (≤ 8.5 mm) (HR 5.21), and bone augmentation (HR 2.58) were independent predictors of success rate. Bone augmentation (HR 5.14) and narrow implants (≤ 3.3 mm) (HR 3.86) were independent predictors of the survival rate. CONCLUSION: Male sex, shorter or narrow implants, and bone augmentation were independent risk factors for dental implants used in dentomaxillary prostheses in patients with large bone defects. CLINICAL RELEVANCE: Clinicians should consider these risk factors and pay close attention to the management of these patients.

  Mar. 2022, Clinical oral investigations, 26(3) (3), 2743 - 2750, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Risk factors associated with post-extraction bleeding in patients on warfarin or direct-acting oral anticoagulants: a retrospective cohort study.

  Eiji Iwata, Akira Tachibana, Junya Kusumoto, Takumi Hasegawa, Ryo Kadoya, Yui Enomoto, Naoki Takata, Masaya Akashi

  PURPOSE: The purpose of this study was to investigate the risk factors associated with post-extraction persistent bleeding in patients on warfarin or direct-acting oral anticoagulants (DOACs) and the ability of risk scores to predict post-extraction bleeding. METHODS: Three hundred ninety-one patients taking warfarin or DOACs underwent tooth extractions. Various risk factors for post-extraction bleeding, including number of tooth extraction, with antiplatelet therapy, and risk scores, were investigated by univariate and multivariate analyses. A post-extraction bleeding was classified into grades 1-3. RESULTS: The incidence of post-extraction bleeding was 26.8% (77 out of 287 patients; grade 1: 63, grade 2:14) in patients taking warfarin, and 26.0% (27 out of 104 patients; grade 1: 20, grade 2:7) in patients taking warfarin DOACs. Multivariate analyses showed that multiple teeth extractions and HAS-BLED scores (above 3 points) in patients taking warfarin, and only multiple teeth extractions in patients taking DOAC, were significantly associated with post-extraction bleeding, respectively. CONCLUSION: Most of the post-extraction bleedings were grade 1, which can be stopped by eligibly pressing gauze by surgeons. If patients taking anticoagulants are scheduled to undergo multiple teeth extractions or their HAS-BLED score are above 3 points (if warfarin), we recommend informing patients risk of post-extraction bleeding before operation, taking carefully hemostasis, and instructing patients to bite down accurately on the gauze for longer than usual.

  Jan. 2022, Oral and maxillofacial surgery, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Actinomycosis of the Lower Lip: Report of a Case.

  Manabu Shigeoka, Daisuke Takeda, Masaya Akashi

  Actinomycosis is usually a chronic infectious disease caused by Actinomyces species, which are anaerobic Gram-positive bacteria that normally colonize the human oral cavity and digestive and urogenital tracts. Although this lesion often occurs on soft tissue around the mandible, cases localized in the lip are very uncommon. We encountered a patient with actinomycosis in the lower lip. A 76-year-old woman with a 5-mm submucosal nodule of the lower lip was referred to our hospital from a dental clinic. The clinical diagnosis was a benign submucosal tumor. Total excision and histological examination were conducted. No oral antibiotic therapy was prescribed. The histological diagnosis was actinomycosis. The postoperative course was uneventful with no signs of recurrence during the 6 months after surgery.

  2022, Case reports in dentistry, 2022, 6121315 - 6121315, English, International magazine

  Link to Kobe Univ. Repository (Kernel)


• A Case of a Rare Branching Pattern in the Carotid Artery.

  Ayumi Sakai, Kazunobu Hashikawa, Akiko Sakakibara, Nobuyuki Murai, Tadashi Nomura, Masaya Akashi, Hiroto Terashi

  2022, Eplasty, 22, ic1, English, International magazine


• アカデミア臨床研究環境のこれまで 臨床研究法施行の背景

  筧 康正, 永井 洋士, 長谷川 巧実, 明石 昌也

  わが国における臨床研究環境は近年著しく変化している。なかでも2018年4月1日に臨床研究法が施行されたことは大きな転換点といえる。その背景として2013年から2014年にかけて、ディオバン事件を代表とする臨床研究に関する不適切な事案が複数生じたことが挙げられる。それらの臨床研究では、複数の大学機関が関連していたデータ操作・不透明な奨学寄附金を通じた研究者と製薬企業との関係性が大きくクローズアップされ、社会問題化した。これらの研究不正に鑑みて、厚労省は、臨床研究の信頼の回復のためには法規制が必要との結論に達し、倫理指針の改定・厳格化を経て臨床研究法施行の運びとなった。臨床研究に関わる全てのものにとって本法の要請を理解し、適切に対応することが求められている一方で、本法を理解するための文書類の多さや手続きの煩雑さなど、研究者の負担が大きく増えたことから、臨床試験の停滞や萎縮を招いている。本法の大きな特徴として、研究責任医師に臨床研究の責任が集約されたこと、研究者は厚労省が認定した認定臨床研究審査委員会を通して試験計画を厚労省に届けること、利益相反の流れが詳細に定められたことなどが挙げられる。本報告では臨床研究倫理における臨床研究法成立までの臨床研究環境の変化について述べたうえで、臨床研究法について概説する。(著者抄録)

  (一社)日本口腔腫瘍学会, Dec. 2021, 日本口腔腫瘍学会誌, 33(4) (4), 159 - 163, Japanese


• アカデミア臨床研究環境のこれまで 臨床研究法施行の背景

  筧 康正, 永井 洋士, 長谷川 巧実, 明石 昌也

  わが国における臨床研究環境は近年著しく変化している。なかでも2018年4月1日に臨床研究法が施行されたことは大きな転換点といえる。その背景として2013年から2014年にかけて、ディオバン事件を代表とする臨床研究に関する不適切な事案が複数生じたことが挙げられる。それらの臨床研究では、複数の大学機関が関連していたデータ操作・不透明な奨学寄附金を通じた研究者と製薬企業との関係性が大きくクローズアップされ、社会問題化した。これらの研究不正に鑑みて、厚労省は、臨床研究の信頼の回復のためには法規制が必要との結論に達し、倫理指針の改定・厳格化を経て臨床研究法施行の運びとなった。臨床研究に関わる全てのものにとって本法の要請を理解し、適切に対応することが求められている一方で、本法を理解するための文書類の多さや手続きの煩雑さなど、研究者の負担が大きく増えたことから、臨床試験の停滞や萎縮を招いている。本法の大きな特徴として、研究責任医師に臨床研究の責任が集約されたこと、研究者は厚労省が認定した認定臨床研究審査委員会を通して試験計画を厚労省に届けること、利益相反の流れが詳細に定められたことなどが挙げられる。本報告では臨床研究倫理における臨床研究法成立までの臨床研究環境の変化について述べたうえで、臨床研究法について概説する。(著者抄録)

  (一社)日本口腔腫瘍学会, Dec. 2021, 日本口腔腫瘍学会誌, 33(4) (4), 159 - 163, Japanese


• 口腔癌術後の再建組織より採取した分層植皮により顎堤形成術を施行した1例

  重永 一輝, 有本 智美, 武田 大介, 木本 明, 長谷川 巧実, 明石 昌也

  (公社)日本顎顔面インプラント学会, Nov. 2021, Japanese Journal of Maxillo Facial Implants, 20(3) (3), 189 - 189, Japanese


• 放射線下顎骨壊死に対する腓骨皮弁再建後にインプラントによる咬合回復を行った1例

  武田 大介, 有本 智美, 長谷川 巧実, 明石 昌也

  (公社)日本顎顔面インプラント学会, Nov. 2021, Japanese Journal of Maxillo Facial Implants, 20(3) (3), 227 - 227, Japanese


• Local Application of Transcutaneous Carbon Dioxide Paste Decreases Inflammation and Accelerates Wound Healing.

  Rika Amano-Iga, Takumi Hasegawa, Daisuke Takeda, Aki Murakami, Nanae Yatagai, Izumi Saito, Satomi Arimoto, Yasumasa Kakei, Akiko Sakakibara, Masaya Akashi

  INTRODUCTION:  Delayed wound healing after surgery lowers the long-term quality of a patient's life and leads to discomfort and pain. However, treatments for wound healing are often difficult and have not yet been fully established. In this study, we investigated the effect of a special paste that can be administered transdermally and holds a non-gaseous carbon dioxide (CO2) source in its carrier, which can be applied to the head and neck region for wound healing in a rat skin defect model. METHODS: Forty-eight Sprague Dawley rats were randomized into control and CO2 groups. We punched a 6.2-mm wound on the back of each rat. The control rats were left untreated, whereas rats in the CO2 group were treated with the CO2 paste every day after surgery. We evaluated wound healing 3, 7, 14, and 21 days after wounding by analyzing the diameter of the wound, gene expression of inflammatory markers vascular endothelial growth factor (VEGF), transforming growth factor (TGF)-β, hypoxia-inducible factor (HIF)-1α, interleukin (IL)-1β, and IL-6 using quantitative real-time polymerase chain reaction, hematoxylin and eosin, and immunohistochemical staining patterns. RESULTS: Rats in the CO2 group showed accelerated wound healing compared to those in the control group. Furthermore, VEGF and TGF-β were overexpressed, whereas HIF-1α, IL-1β, and IL-6 were downregulated in the rats treated with CO2. Immunohistochemical analysis also revealed similar patterns of expression. CONCLUSION: Taken together, the CO2 paste promoted wound healing by regulating the hypoxic environment, reducing inflammation, and accelerating angiogenesis.

  Nov. 2021, Cureus, 13(11) (11), e19518, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Risk factors for severe radiation-induced oral mucositis in patients with oral cancer

  Sakiko Soutome, Souichi Yanamoto, Mika Nishii, Yuka Kojima, Takumi Hasegawa, Madoka Funahara, Masaya Akashi, Toshiyuki Saito, Masahiro Umeda

  Background/purpose: Radiotherapy for head and neck cancer often causes severe oral mucositis. The purpose of this retrospective study was to further examine the risk factors for developing severe oral mucositis in patients with oral cancer undergoing radiotherapy as a compliment to a previous study performed by our group. Materials and methods: A total of 181 patients with oral cancer undergoing radiotherapy were enrolled in the study. The association between a number of potential risk factors and grade 3 oral mucositis were analyzed using the cox proportional hazard model and a logistic regression analysis. Results: Grade 3 oral mucositis occurred in 56 patients. The cox proportional hazard model analysis revealed that those with lower hemoglobin levels, concurrent cisplatin and cetuximab administration, and a larger number of teeth showed a significantly higher incidence of severe oral mucositis. Logistic regression analysis revealed that patients who had lower hemoglobin levels, received concurrent cisplatin or cetuximab treatment, and were not administered pilocarpine showed a significantly higher incidence of severe oral mucositis. The presence of teeth may stimulate the oral mucosa and become a risk factor for mucositis, and the administration of pilocarpine might reduce the risk. Conclusion: This study describes the risk factors of severe radiation-induced oral mucositis in oral cancer patients and shows the possibility of risk reduction by pilocarpine. This information could help patients avoid painful mucositis.

  Oct. 2021, Journal of Dental Sciences, 16(4) (4), 1241 - 1246

  Scientific journal


• Sturge-Weber症候群に伴う口腔内化膿性肉芽腫の治療経験:術前塞栓療法と外科的切除の併用

  酒井 亜結美, 野村 正, 明石 昌也, 橋川 和信, 寺師 浩人

  57歳女性。19歳時にSturge-Weber症候群を発症し、4年前より右上歯肉に赤色病変が出現した。数ヵ月前から病変が急速に増大し、病変による咀嚼困難、構音障害を生じたため当院へ受診となった。受診時、悪臭や拍動を伴う易出血性の右上歯肉病変が口腔内を占拠し、顔面造影CTおよび血管造影検査では病変は大口蓋動脈と顔面動脈から豊富に栄養され、硬口蓋の広範囲に及んでいた。症状の緩和目的で口腔内に突出した病変を切除する方針となったが、右上歯肉病変は止血困難であることが予想されたため、まず動脈塞栓療法を行い、次いで外科的切除術が行われた。その結果、病理組織学的に化膿性肉芽腫と診断された。術後6ヵ月経過現在、病変の再増大や再出血はなく、咀嚼も構音も改善した。

  (一社)日本形成外科学会, Sep. 2021, 日本形成外科学会会誌, 41(9) (9), 522 - 527, Japanese


• Peripheral facial nerve schwannoma at the inferior mandibular margin: a case report.

  Akiko Sakakibara, Takumi Hasegawa, Daisuke Takeda, Junya Kusumoto, Shunsuke Sakakibara, Masaya Akashi

  Schwannomas commonly occur in the head and neck region as acoustic neuromas. Facial nerve schwannomas are rare and usually occur in the temporal region. A 57-year-old woman presented with a mass at the right mandibular margin. Magnetic resonance imaging revealed a schwannoma located immediately caudal to the mental foramen. We were initially uncertain whether it arose from the trigeminal nerve or the facial nerve. Excision was performed under general anesthesia. The mass was encapsulated and easily detached from the surrounding tissue. The nerve of origin was identified proximal to the tumor. A facial nerve origin was confirmed as the muscles supplied by the marginal mandibular branch of the facial nerve moved on nerve stimulation. Nerve fibers were not found distal to the tumor, possibly because they had been cut during excision. We believe that this is the first report of a schwannoma arising from the peripheral facial nerve.

  Jul. 2021, Journal of surgical case reports, 2021(7) (7), rjab299, English, International magazine

  Link to Kobe Univ. Repository (Kernel)


• 皮膚充填材への感染により生じた頬部蜂窩織炎の1例

  榎本 由依, 橘 進彰, 格谷 僚, 岩田 英治, 高田 直樹, 明石 昌也

  71歳女性。右側頬部の腫脹と開口障害を主訴に前医より歯性感染症を疑われ、当院へ紹介となった。初診時、右側頬部皮膚の腫脹と一致して、右側頬粘膜にも腫脹が認められたが、原因となる歯科疾患はみられなかった。その後、詳細な問診で4年前に両側頬部にバイオアルカミドによる除皺術を受けたことが判明した。MRI所見より頬部の皮膚充填材への感染に起因する右側頬部蜂窩織炎と診断され、治療としてSBT/ABPCの投与を行った結果、炎症所見や腫脹、開口障害の改善が得られた。

  (公社)日本口腔外科学会, Jul. 2021, 日本口腔外科学会雑誌, 67(7) (7), 447 - 451, Japanese


• 皮膚充填材への感染により生じた頬部蜂窩織炎の1例

  榎本 由依, 橘 進彰, 格谷 僚, 岩田 英治, 高田 直樹, 明石 昌也

  (公社)日本口腔外科学会, Jul. 2021, 日本口腔外科学会雑誌, 67(7) (7), 447 - 451, Japanese


• Tongue Cancer Cell-Derived CCL20 Induced by Interaction With Macrophages Promotes CD163 Expression on Macrophages

  Manabu Shigeoka, Yu-ichiro Koma, Takayuki Kodama, Mari Nishio, Masaya Akashi, Hiroshi Yokozaki

  CD163-positive macrophages contribute to the aggressiveness of oral squamous cell carcinoma. We showed in a previous report that CD163-positive macrophages infiltrated not only to the cancer nest but also to its surrounding epithelium, depending on the presence of stromal invasion in tongue carcinogenesis. However, the role of intraepithelial macrophages in tongue carcinogenesis remains unclear. In this study, we assessed the biological behavior of intraepithelial macrophages on their interaction with cancer cells. We established the indirect coculture system (intraepithelial neoplasia model) and direct coculture system (invasive cancer model) of human monocytic leukemia cell line THP-1-derived CD163-positive macrophages with SCC25, a tongue squamous cell carcinoma (TSCC) cell line. Conditioned media (CM) harvested from these systems were analyzed using cytokine array and enzyme-linked immunosorbent assay and extracted a specific upregulated cytokine in CM from the direct coculture system (direct CM). The correlation of both this cytokine and its receptor with various clinicopathological factors were evaluated based on immunohistochemistry using clinical samples from 59 patients with TSCC. Moreover, the effect of this cytokine in direct CM on the phenotypic alterations of THP-1 was confirmed by real-time polymerase chain reaction, western blotting, immunofluorescence, and transwell migration assay. It was shown that CCL20 was induced in the direct CM specifically. Interestingly, CCL20 was produced primarily in SCC25. The expression level of CCR6, which is a sole receptor of CCL20, was higher than the expression level of SCC25. Our immunohistochemical investigation showed that CCL20 and CCR6 expression was associated with lymphatic vessel invasion and the number of CD163-positive macrophages. Recombinant human CCL20 induced the CD163 expression and promoted migration of THP-1. We also confirmed that a neutralizing anti-CCL20 antibody blocked the induction of CD163 expression by direct CM in THP-1. Moreover, ERK1/2 phosphorylation was associated with the CCL20-driven induction of CD163 expression in THP-1. Tongue cancer cell-derived CCL20 that was induced by interaction with macrophages promotes CD163 expression on macrophages.

  Frontiers Media SA, Jun. 2021, Frontiers in Oncology, 11

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A Case Report of Tongue Lymphoepithelial Carcinoma with a Histological Diagnostic Dilemma.

  Daisuke Takeda, Manabu Shigeoka, Tenyu Sugano, Nanae Yatagai, Takumi Hasegawa, Masaya Akashi

  Most head and neck lymphoepithelial carcinomas (LECs) arise in the nasopharynx and harbor Epstein-Barr virus (EBV). LEC is also a rare subtype of the oral squamous cell carcinoma (SCC). Morphologically, LEC is defined as resembling non-keratinizing nasopharyngeal carcinoma, undifferentiated subtype. The histological features and pathogenesis of oral LEC are not established. We describe a case of tongue LEC with histopathological diagnostic difficulties. A 72-year-old Japanese female presented with a whitish change on her left-side tongue. The diagnosis was atypical epithelium; neoplastic change could not be ruled out by a biopsy. Although the lesion was monitored at our hospital per her request, invasive carcinoma was detected 11 months later. Microscopically, conventional SCC was observed with the characteristic features as LEC confined to the deep part of the lesion. We briefly discuss this unusual histological finding and make a novel proposal for distinguishing oral LEC from LECs in other regions based on these histological findings.

  Jun. 2021, Diagnostics (Basel, Switzerland), 11(6) (6), English, International magazine

  Link to Kobe Univ. Repository (Kernel)


• A Case Report of Tongue Lymphoepithelial Carcinoma with a Histological Diagnostic Dilemma

  Daisuke Takeda, Manabu Shigeoka, Tenyu Sugano, Nanae Yatagai, Takumi Hasegawa, Masaya Akashi

  Most head and neck lymphoepithelial carcinomas (LECs) arise in the nasopharynx and harbor Epstein-Barr virus (EBV). LEC is also a rare subtype of the oral squamous cell carcinoma (SCC). Morphologically, LEC is defined as resembling non-keratinizing nasopharyngeal carcinoma, undifferentiated subtype. The histological features and pathogenesis of oral LEC are not established. We describe a case of tongue LEC with histopathological diagnostic difficulties. A 72-year-old Japanese female presented with a whitish change on her left-side tongue. The diagnosis was atypical epithelium; neoplastic change could not be ruled out by a biopsy. Although the lesion was monitored at our hospital per her request, invasive carcinoma was detected 11 months later. Microscopically, conventional SCC was observed with the characteristic features as LEC confined to the deep part of the lesion. We briefly discuss this unusual histological finding and make a novel proposal for distinguishing oral LEC from LECs in other regions based on these histological findings.

  MDPI, Jun. 2021, DIAGNOSTICS, 11(6) (6), English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Denosumab-related osteonecrosis of the jaw after tooth extraction and the effects of a short drug holiday in cancer patients: a multicenter retrospective study.

  T Hasegawa, N Ueda, S I Yamada, S Kato, E Iwata, S Hayashida, Y Kojima, M Shinohara, I Tojo, H Nakahara, T Yamaguchi, T Kirita, H Kurita, Y Shibuya, S Soutome, M Akashi

  Pre-existing inflammation, corticosteroid therapy, periapical periodontitis, longer duration of denosumab therapy, and female sex were significantly associated with an increased risk of denosumab-related osteonecrosis of the jaw after tooth extraction in patients with cancer on oncologic doses of denosumab. A short drug holiday did not protect against this complication. INTRODUCTION: This study retrospectively investigated the relationship between various risk factors, including brief discontinuation of denosumab, and development of denosumab-related osteonecrosis of the jaw (DRONJ) after tooth extraction in patients with cancer who were receiving oncologic doses of this agent. METHODS: Data were collected on demographic characteristics, duration of denosumab therapy, whether or not denosumab was discontinued before tooth extraction (drug holiday), duration of discontinuation, presence of pre-existing inflammation, and whether or not additional surgical procedures were performed. Risk factors for DRONJ after tooth extraction were evaluated by univariate and multivariate analyses. RESULTS: A total of 136 dental extractions were performed in 72 patients (31 men, 41 women) with cancer who were receiving oncologic doses of denosumab. Post-extraction DRONJ was diagnosed in 39 teeth (28.7%) in 25 patients. Tooth extraction was significantly associated with development of DRONJ only in patients with pre-existing inflammation (odds ratio [OR] 243.77), those on corticosteroid therapy (OR 73.50), those with periapical periodontitis (OR 14.13), those who had been taking oncologic doses of denosumab for a longer period (OR 4.69), and in women (OR 1.04). There was no significant difference in the occurrence of DRONJ between patients who had a drug holiday before tooth extraction and those who did not. CONCLUSIONS: These findings suggest that inflamed teeth should be extracted immediately in patients with cancer who are receiving oncologic doses of denosumab. Drug holidays have no significant impact on the risk of DRONJ.

  May 2021, Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Alteration of Macrophage Infiltrating Compartment: A Novel View on Oral Carcinogenesis

  Manabu Shigeoka, Yu-ichiro Koma, Mari Nishio, Masaya Akashi, Hiroshi Yokozaki

  <b><i>Background:</i></b> The mortality of oral squamous cell carcinoma (OSCC) has remained high for decades; therefore, methods for early detection of OSCC are warranted. However, in the oral cavity, various mucosal diseases may be encountered, including reactive lesions and oral potentially malignant disorders, and it is difficult to differentiate OSCC from these lesions based on both clinical and histopathological findings. It is well known that chronic inflammation contributes to oral cancer development. Macrophages are among the most common inflammatory cells in cancer stromal tissue and have various roles in cancer aggressiveness. Although the roles of macrophages in cancer development have attracted attention, only a few studies have linked macrophages to carcinogenesis, particularly, oral precancerous lesions. <b><i>Summary:</i></b> This review article consists of 3 parts: first, we summarize current knowledge on macrophages in human various epithelial precancerous lesions, excluding the oral cavity, to show the importance and gaps in knowledge regarding macrophages in carcinogenesis; second, we review published data related to the role of macrophages in oral carcinogenesis; finally, we present a novel view on oral carcinogenesis, focusing on crosstalk between epithelial cells and macrophages. <b><i>Key Messages:</i></b> The biological features of macrophages in oral carcinogenesis differ drastically depending on the anatomical compartment that they infiltrate. Focusing on the alteration of macrophage infiltrating compartment may serve as a useful novel approach for studying the role of the macrophages in oral carcinogenesis and for gaining further insight into cancer prevention and early detection.

  S. Karger AG, May 2021, Pathobiology, 1 - 11

  Scientific journal


• 早期舌癌治療のパラダイムシフトへ向けて-医科歯科病理の知の融合- 早期舌扁平上皮癌における頸部後発転移の新たな予測因子

  山川 延宏, 桐田 忠昭, 梅田 正博, 柳本 惣一, 大鶴 光信, 大倉 正也, 相川 友直, 太田 嘉英, 栗田 浩, 山田 慎一, 明石 昌也, 長谷川 巧, 上田 倫弘

  (一社)日本頭頸部癌学会, May 2021, 頭頸部癌, 47(2) (2), 142 - 142, Japanese


• 口腔・中咽頭癌放射線治療326例における重症口腔粘膜炎および口腔カンジダ症発症に関連する因子

  西井 美佳, 五月女 さき子, 岩田 英治, 長谷川 巧実, 兒島 由佳, 船原 まどか, 梅田 正博, 明石 昌也

  (一社)日本口腔ケア学会, Apr. 2021, 日本口腔ケア学会雑誌, 15(3) (3), 145 - 145, Japanese


• Occurrence and Treatment Outcome of Late Complications After Free Fibula Flap Reconstruction for Mandibular Osteoradionecrosis.

  Junya Yamashita, Masaya Akashi, Daisuke Takeda, Junya Kusumoto, Takumi Hasegawa, Kazunobu Hashikawa

  PURPOSE: This study aimed to evaluate the occurrence and treatment outcome of late complications after free fibula osteocutaneous flap reconstruction for mandibular osteoradionecrosis (ORN). METHODS: We enrolled 15 consecutive patients (14 men, one woman; median age 65 years, range 57-80 years) who underwent free fibula reconstruction for advanced mandibular ORN during 2013-2017 with two or more years of follow-up. Late complications included infection, plate exposure, and recurrence at the resection margin. The effect of perioperative antibiotic administration on late complications was also assessed. RESULTS: Late complications occurred in 33.3% (5/15) of patients, including two infections (local and distant), two plate exposures, and two recurrences (plate exposure and recurrence occurred in one patient). Perioperative antibiotic administration duration did not significantly affect the occurrence of postoperative late complications. All late complications were treated without problems. CONCLUSIONS: Late complications after ORN reconstructive surgery are not uncommon, but can be treated properly.

  Corresponding, CUREUS INC, Mar. 2021, Cureus, 13(3) (3), e13833, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Impact of Oral Health Status on Postoperative Complications and Functional Recovery After Cardiovascular Surgery

  Masato Ogawa, Seimi Satomi-Kobayashi, Naofumi Yoshida, Yasunori Tsuboi, Kodai Komaki, Nagisa Nanba, Kazuhiro P. Izawa, Takeshi Inoue, Yoshitada Sakai, Masaya Akashi, Ken ichi Hirata, Kenji Okada

  Background: Poor oral health status can lead to a deteriorated level of general health and is common among patients undergoing cardiovascular surgery. However, the effect of oral health status on postoperative outcomes in cardiovascular surgery patients remains unclear. Thus, we investigated the effect of preoperative oral health status on postoperative complications and functional recovery after cardiovascular surgery. Methods: This single-centre retrospective cohort study included 884 inpatients undergoing elective cardiovascular surgery. Oral health status was assessed based on the number of remaining teeth, use of dentures, occlusal support, and periodontal status. We investigated postoperative complications related to surgery and postoperative functional recovery by measuring the reacquisition of walking ability, activities of daily living, and length of postoperative hospital stay. Results: In this cohort (age 66.9 ± 13.4 years), the mean number of remaining teeth was 18.7 ± 9.4. Patients were grouped based on tertiles of the data distribution of remaining teeth: ≥ 20 teeth (470 patients); 10-19 teeth (137 patients); < 10 teeth (185 patients). The number of missing teeth was associated with age (P < 0.001). The prevalence of postoperative pneumonia and reintubation after surgery was 3.2% and 2.5%, respectively, which was significantly higher in patients with severe tooth loss (P < 0.05 for both). After adjusting for age and other confounding factors, the number of remaining teeth was a statistically significant predictor of functional recovery (P < 0.05). Conclusions: Preoperative oral health status was related to postoperative respiratory complications and independently associated with functional recovery. Preoperative oral intervention may improve functional recovery after cardiovascular surgery.

  Mar. 2021, CJC Open, 3(3) (3), 276 - 284

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Can CT predict the development of oroantral fistula in patients undergoing maxillary third molar removal?

  Eiji Iwata, Takumi Hasegawa, Masaki Kobayashi, Akira Tachibana, Naoki Takata, Toshiya Oko, Daisuke Takeda, Yoshiki Ishida, Tsuyoshi Fujita, Ikuko Goto, Junichiro Takeuchi, Masaya Akashi

  PURPOSE: In maxillary wisdom tooth extraction, the necessity of CT is unknown. The purpose of this study was to investigate whether CT adding to orthopantomography is useful for predicting oroantral perforation during maxillary third molar extraction. METHODS: Various risk factors for oroantral perforation during maxillary third molar extraction were investigated by univariate and multivariate analyses. We analyzed those of all patients and the patients who underwent CT, respectively. The proximity of the roots to the maxillary sinus floor (root-sinus [RS] classification) and Archer classification were assessed using panoramic radiography. The number of roots and vertical relationship were assessed using CT. RESULTS: A total of 604 out of 3299 patients underwent CT adding to orthopantomography. In all cases, multivariate analyses except for CT findings showed that the RS classification type III/IV and the Archer classification Type B/C/D in panoramic findings were significantly correlated with oroantral perforation as radiological findings. In cases for which CT was performed, multivariate analyses showed that one root (OR 12.87) and the vertical relationship Type D (OR 5.63) in CT findings, besides the RS classification type III/IV (OR 4.47) in panoramic findings, were significantly related to oroantral perforation. CONCLUSION: The RS classification and the Archer classification in panoramic findings can predict the risk of oroantral perforation. The usefulness of CT adding to orthopantomography is limited. However, when the relationship between the upper wisdom tooth and maxillary sinus floor (RS classification) is unclear, to check whether the number of roots is one and the apex of one root is projecting into the maxillary sinus in CT findings, is useful for the prediction.

  Mar. 2021, Oral and maxillofacial surgery, 25(1) (1), 7 - 17, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Association between dental extraction after radiotherapy and osteoradionecrosis: A multi-centre retrospective study.

  Izumi Saito, Takumi Hasegawa, Yumiko Kawashita, Shinichiro Kato, Shin-Ichi Yamada, Yuka Kojima, Nobuhiro Ueda, Masahiro Umeda, Yasuyuki Shibuya, Hiroshi Kurita, Tadaaki Kirita, Masaya Akashi

  OBJECTIVE: Radiotherapy (RT) carries a substantial risk for the development of osteoradionecrosis (ORN) of the jaw. This study was performed to investigate the relationship between dental extractions after RT and the development of ORN. MATERIAL AND METHODS: Thirty-two patients with head and neck cancer who underwent tooth extraction after RT were investigated for correlations between the development of ORN and various factors. RESULTS: Postextraction ORN was diagnosed in 12 (12.1%) teeth of 9 patients. The RT dose against the site of tooth extraction was 62.0 and 37.4 Gy in the ORN and Non-ORN groups, respectively (p < .001). The duration from RT to tooth extraction was 41.2 and 28.2 months in the ORN and Non-ORN groups, respectively (p = .025). Tooth extraction was significantly associated with ORN in patients with a high RT dose against the site (odds ratio = 1.231) and a longer duration of time from RT (odds ratio = 1.084). CONCLUSIONS: Extraction of non-restorable teeth and those with a poor prognosis should not necessarily be postponed even when patients are undergoing RT. However, clinicians should pay special attention to postoperative management after tooth extraction in patients with a high RT dose and longer time from RT.

  Mar. 2021, Oral diseases, 28(4) (4), 1181 - 1187, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Time to Recurrence Associated With Poor Prognosis in Japanese Oral Squamous Cell Carcinoma Patients

  Takumi Hasegawa, Erina Kobayashi, Rika Amano, Izumi Saito, Daisuke Takeda, Yasumasa Kakei, Akira Kimoto, Akiko Sakakibara, Masaya Akashi

  Purpose Recurrence in oral squamous cell carcinoma (OSCC) is not rare. Due to lack of studies assessing characteristics of recurrent OSCC, including time to recurrence and outcomes, we sought to investigate its characteristics, time to recurrence, and outcomes in Japanese OSCC patients. Methods This study was a nonrandomized retrospective cohort study in a tertiary referral centre. It included 208 (117 men and 91 women) patients with recurrent oral cancer who underwent major curative surgery in the Department of Oral and Maxillofacial Surgery at Kobe University Hospital between January 1999 and April 2017. The outcomes were disease-specific survival (DSS) and overall survival (OS). Results In multivariable Cox proportional hazards analysis, the time to recurrence (hazard ratio [HR] 3.55, 95% confidence interval [CI] 1.69-6.63; P = 0.001), extranodal extension (ENE, HR 2.72, 95% CI 1.51-4.89; P = 0.001), and high T stage (HR 2.00, 95% CI 1.01-3.97; P = 0.046) were independent predictors of DSS. The time to recurrence (HR 3.29, 95% CI 1.82-5.96; P < 0.001) and ENE (HR 2.64, 95% CI 1.52-4.56; P = 0.001) were independent predictors of OS. Conclusion Time to recurrence, extranodal extension, and higher T stage were independent prognosis predictors in OSCC.

  SPRINGER INDIA, Feb. 2021, JOURNAL OF MAXILLOFACIAL & ORAL SURGERY, 21(3) (3), 856 - 864, English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Relationship between oral health and physical frailty in patients with cardiovascular disease.

  Masato Ogawa, Seimi Satomi-Kobayashi, Naofumi Yoshida, Yasunori Tsuboi, Kodai Komaki, Nagisa Nanba, Kazuhiro P Izawa, Yoshitada Sakai, Masaya Akashi, Ken-Ichi Hirata

  BACKGROUND: Oral health is important for maintaining general health and is associated with components of physical frailty among the elderly. Oral health problems are common in hospitalized patients; however, no reports on oral health problems pertain to patients with cardiovascular diseases (CVD). The present study aimed to evaluate the association between oral health and physical frailty in these patients. METHODS: In this retrospective cohort study, we included consecutive patients admitted for CVD to our hospital between May 2014 and December 2018. Physical frailty was assessed using the Short Physical Performance Battery (SPPB). Oral health characteristics, such as the number of remaining teeth, denture use, occlusal support, and periodontal status, were assessed. RESULTS: In our cohort (n = 457), 111 (24.3%) patients had physical frailty. Univariate linear regression showed that the number of teeth present and the prevalence of occlusal support were significantly lower in patients with than without physical frailty. Pearson correlation indicated that the number of teeth significantly correlated with the nutritional status (r = 0.27) and SPPB score (r = 0.24), grip strength (r = 0.33), and 6-minute walking distance (r = 0.26). Multiple linear regression analysis showed that the number of teeth was independently associated with physical frailty after adjusting for confounders. CONCLUSIONS: Oral health was closely associated with physical frailty, and nutritional status in patients with CVD; thus, it could be an important screening marker for early frailty symptoms and a predictor of future malnutrition risk.

  Feb. 2021, Journal of cardiology, 77(2) (2), 131 - 138, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of preoperative dental examination and oral hygiene instruction on surgical site infection after hepatectomy: a retrospective study.

  Takumi Hasegawa, Daisuke Takeda, Motofumi Tanaka, Rika Amano, Izumi Saito, Yasumasa Kakei, Akira Kimoto, Takumi Fukumoto, Masaya Akashi

  PURPOSE: This study retrospectively investigated relationships among risk factors and post-hepatectomy surgical site infection (SSI) and other complications in patients who underwent hepatectomy for hepatocellular carcinoma (HCC). METHODS: We included 334 patients who underwent hepatectomies for liver cancers between January 2011 and December 2015 in this study. We evaluated risk factors for SSI and other post-hepatectomy complication, including demographic factors, preoperative factors, and preoperative intervention including oral management, perioperative factors, and length of hospital stay, with univariate and multivariate analyses. The oral management intervention included self-care instructions, extraction of infected teeth, removal of dental plaques and calculus (scaling), professional mechanical teeth cleaning, removal of tongue coating, and cleaning of dentures. SSI was defined in accordance with the guideline issued by the Centers for Disease Control and Prevention; it included purulent discharge from any incision or organ space within 30 days postoperatively, with or without microbiological evidence. Complications of grade II or greater, according to the Clavien-Dindo classification, were regarded as postoperative complications. RESULTS: We found bacterial infection of ascites (Odds ratio (OR) = 13.72), lack of preoperative oral management intervention (OR = 10.17), and severe liver fibrosis (OR = 2.76) to be associated with SSI and severe liver fibrosis (OR = 2.28), hypoalbuminemia (OR = 2.02), blood transfusion (OR = 1.86), and longer operation time (OR = 1.80) to be associated with postoperative complications. CONCLUSIONS: Preoperative oral management may reduce the risk of SSI in patients with HCC who undergo hepatectomy.

  Feb. 2021, Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer, 29(2) (2), 653 - 659, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The prospective evaluation and risk factors of dysphagia after surgery in patients with oral cancer.

  Takumi Hasegawa, Nanae Yatagai, Tatsuya Furukawa, Emi Wakui, Izumi Saito, Daisuke Takeda, Yasumasa Kakei, Akiko Sakakibara, Ken-Ichi Nibu, Masaya Akashi

  BACKGROUND: This prospective study investigated the change of swallowing ability using the Swallowing Ability Scale System (SASS) and swallowing-related quality of life (QOL) by Performance Status Scale for Head and Neck Cancer patients (PSS-H&N). This study also investigated the risk factors for postoperative dysphagia in patients who received reconstructive surgery for oral cancer. SUBJECTS AND METHODS: This study included 64 patients (33 men and 31 women) who underwent radical surgery with neck dissection and reconstructive surgery for oral cancers between July 2014 and February 2018. We evaluated risk factors for poor swallowing ability after treatment, including demographic factors, preoperative factors and perioperative factors, with univariate and multivariate analyses. The change of swallowing ability by the SASS and swallowing-related QOL by PSS-H&N were evaluated prospectively prior to the initiation of surgery within 1 week and at 1 and 3 months after treatment. RESULTS: Advanced T stage (T3, 4) (odds ratio (OR) = 79.71), bilateral neck dissection (OR = 20.66) and the resection of unilateral or bilateral suprahyoid muscles (OR = 17.00) were associated with poor swallowing ability after treatment. The scores for time for food intake and Eating in Public were associated with decrease of QOL in the poor group. CONCLUSIONS: We propose that clinicians consider the risk factors identified in this study and pay close attention to the management of oral cancer patients with reconstructive surgery.

  Jan. 2021, Journal of otolaryngology - head & neck surgery = Le Journal d'oto-rhino-laryngologie et de chirurgie cervico-faciale, 50(1) (1), 4 - 4, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effect of educational intervention on the appropriate use of oral antimicrobials in oral and maxillofacial surgery: a retrospective secondary data analysis.

  Junya Kusumoto, Atsushi Uda, Takeshi Kimura, Shungo Furudoi, Ryosuke Yoshii, Megumi Matsumura, Takayuki Miyara, Masaya Akashi

  BACKGROUND: In Japan, oral third-generation cephalosporins with broad-spectrum activity are commonly prescribed in the practices of dentistry and oral surgery. However, there are few reports on the appropriate use of antibiotics in the field of oral surgery. This study aimed to evaluate the appropriateness of antibiotic use before and after an educational intervention in the Department of Oral and Maxillofacial Surgery, Kobe University Hospital. METHODS: The use of oral antibiotics was investigated among inpatients and outpatients before and after an educational intervention conducted by the antimicrobial stewardship team. Additionally, the frequency of surgical site infection after the surgical removal of an impacted third mandibular molar under general anesthesia and the prevalence of adverse effects of the prescribed antibiotics were comparatively evaluated between 2013 and 2018. RESULTS: After the educational intervention, a remarkable reduction was noted in the prescription of oral third-generation cephalosporins, but increased use of penicillins was noted among outpatients. There was reduced use of macrolides and quinolones in outpatients. Although a similar trend was seen for inpatients, the use of quinolones increased in this population. Despite the change in the pattern of antibiotic prescription, inpatients who underwent mandibular third molar extraction between 2013 and 2018 did not show a significant increase in the prevalence of surgical site infections (6.2% vs. 1.8%, p = .336) and adverse effects of drugs (2.1% vs. 0%, p = .466). CONCLUSIONS: This study suggests that the judicious use of oral antibiotics is possible through conscious and habitual practice of appropriate antibiotic use. However, further investigation is required to develop measures for appropriate use of oral antibiotics.

  Jan. 2021, BMC oral health, 21(1) (1), 20 - 20, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Risk factors for osteoradionecrosis of the jaw in patients with head and neck squamous cell carcinoma.

  Hikaru Kubota, Daisuke Miyawaki, Naritoshi Mukumoto, Takeaki Ishihara, Megumi Matsumura, Takumi Hasegawa, Masaya Akashi, Naomi Kiyota, Hirotaka Shinomiya, Masanori Teshima, Ken-Ichi Nibu, Ryohei Sasaki

  BACKGROUND: To evaluate factors associated with osteoradionecrosis of the jaw (ORNJ) in patients with head and neck squamous cell carcinoma (HNSCC), focusing on jaw-related dose-volume histogram (DVH) parameters. METHODS: We retrospectively reviewed the medical records of 616 patients with HNSCC treated with curative-intent or postoperative radiation therapy (RT) during 2008-2018. Patient-related (age, sex, history of smoking or alcohol use, diabetes mellitus, performance status, pre-RT dental evaluation, pre- or post-RT tooth extraction), tumor-related (primary tumor site, T-stage, nodal status), and treatment-related (pre-RT surgery, pre-RT mandible surgery, induction or concurrent chemotherapy, RT technique) variables and DVH parameters (relative volumes of the jaw exposed to doses of 10 Gy-70 Gy [V10-70]) were investigated and compared between patients with and without ORNJ. The Mann-Whitney U test was used to compare RT dose parameters. Univariate and multivariate Cox regression analyses were used to assess factors associated with ORNJ development. Kaplan-Meier analyses were performed for cumulative ORNJ incidence estimation. RESULTS: Forty-six patients (7.5%) developed ORNJ. The median follow-up duration was 40 (range 3-145) months. The median time to ORNJ development was 27 (range 2-127) months. DVH analysis revealed that V30-V70 values were significantly higher in patients with than in those without ORNJ. In univariate analyses, primary tumor site, pre-RT mandible surgery, post-RT tooth extraction, and V60 > 14% were identified as important factors. In multivariate analyses, V60 > 14% (p = 0.0065) and primary tumor site (p = 0.0059) remained significant. The 3-year cumulative ORNJ incidence rates were 2.5% and 8.6% in patients with V60 ≤ 14% and > 14%, respectively (p < 0.0001), and 9.3% and 1.4% in patients with oropharyngeal or oral cancer and other cancers, respectively (p < 0.0001). CONCLUSIONS: V60 > 14% and oropharyngeal or oral cancer were found to be independent risk factors for ORNJ. These findings might be useful to minimize ORNJ incidence in HNSCC treated with curative RT.

  Jan. 2021, Radiation oncology (London, England), 16(1) (1), 1 - 1, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Bacterial Colonization of the Condyle in Patients with Advanced Mandibular Osteoradionecrosis: Analysis of Hemimandibulectomy Specimens.

  Daisuke Takeda, Kazunobu Hashikawa, Manabu Shigeoka, Maki Kanzawa, Nanae Yatagai, Satomi Arimoto, Junya Kusumoto, Takumi Hasegawa, Hiroto Terashi, Masaya Akashi

  Advanced mandibular osteoradionecrosis (ORN) sometimes requires extended resection (e.g., hemimandibulectomy). Bacterial infection contributes to ORN pathogenesis. To control infection and determine the extent of debridement required, an understanding of bacterial spread within sites of mandibular ORN is important. The current study used a histopathological approach to assess bacterial colonization in the mandibular condyle and elucidate possible paths of bacterial spread towards the mandibular condyle. Four hemimandibulectomy specimens were selected. Areas of bone destruction were macroscopically assessed and confirmed using hematoxylin and eosin staining. Bacterial presence within mandibular condyle was confirmed with Gram staining. Bone exposure was observed in the molar area in all specimens. Macroscopic bone destruction was apparent especially near the medial side of the cortical wall. Gram staining revealed bacterial colonization of the mandibular condyle in three of the four specimens. In conclusion, bacteria tended to spread posteriorly and through the medial side of the mandibular cortical wall. In patients with advanced ORN, the potential for bacterial colonization of the mandibular condyle should be considered during treatment.

  Corresponding, 2021, International journal of dentistry, 2021, 9998397 - 9998397, English, International magazine

  Scientific journal


• Transcutaneous Carbon Dioxide Decreases Immunosuppressive Factors in Squamous Cell Carcinoma In Vivo.

  Nanae Yatagai, Takumi Hasegawa, Rika Amano, Izumi Saito, Satomi Arimoto, Daisuke Takeda, Yasumasa Kakei, Masaya Akashi

  INTRODUCTION: In recent years, the tumour immunosuppressive mechanism has attracted attention as a cause of tumour chemoresistance. Although chemoresistance and immunosuppression of tumours have been reported to be associated with a hypoxic environment, effective treatments to improve hypoxia in tumours have not yet been established. We have previously applied carbon dioxide (CO2) to squamous cell carcinoma and have shown that improvement in local oxygenation has an antitumour effect. However, the effects of local CO2 administration on tumour immunosuppression, chemoresistance, and combination with chemotherapy are unknown. In this study, we investigated the effects of local CO2 administration on squamous cell carcinoma and the effects of combined use with chemotherapy, focusing on the effects on tumour immunosuppressive factors. METHODS: Human oral squamous cell carcinoma (HSC-3) was transplanted subcutaneously into the back of a nude mouse, and CO2 and cisplatin were administered. After administration twice a week for a total of 4 times, tumours were collected and the expression of tumour immunosuppressive factors (PD-L1, PD-L2, and galectin-9) was evaluated using real-time polymerase chain reaction and immunostaining. RESULTS: Compared with the control group, a significant decrease in the mRNA expression of PD-L1 was observed in both, CO2-treated and combination groups. Similarly, the expression of PD-L2 and galectin-9 decreased in the CO2-treated and combination groups. Furthermore, immunostaining also showed a significant decrease in the protein expression of tumour immunosuppressive factors in the CO2-treated and combination groups. CONCLUSION: It was confirmed that the tumour immunosuppressive factors decreased due to local CO2 administration to the mouse model. CO2 administration has the potential to improve the hypoxic environment in tumours, and combined use with chemotherapy may also improve tumour immunosuppression.

  2021, BioMed research international, 2021, 5568428 - 5568428, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The characteristics of oro-cervical necrotizing fasciitis-Comparison with severe cellulitis of oro-cervical region and necrotizing fasciitis of other body regions.

  Eiji Iwata, Junya Kusumoto, Naoki Takata, Shungo Furudoi, Akira Tachibana, Masaya Akashi

  BACKGROUND: Necrotizing fasciitis (NF) is an acute and life-threatening soft-tissue infection however rarely seen in oro-cervical region. Therefore, the details of oro-cervical NF (OCNF) are not well known. The purpose of this study was to investigate the characteristics of OCNF by comparing it with severe cellulitis of oro-cervical region (OCSC) or NF of other body regions (e.g., limb, perineum, and trunk) (BNF), respectively. MATERIALS AND METHODS: At first, various risk factors for OCNF in oro-cervical severe infection (OCSI; composed of OCNF and OCSC), including neutrophil-to-lymphocyte ratio (NLR) and Laboratory Risk Indicator for Necrotizing Fasciitis (LRINEC) score, were investigated by univariate and multivariate analyses. Next, the differences between OCNF and BNF, including inflammatory markers and mortality, were investigated. RESULTS: In the present study, 14 out of 231 OCSI patients had OCNF. Multivariate analyses of OCSI patients showed that NLR ≥15.3 and LRINEC score ≥6 points were significantly related to OCNF. During the same period, 17 patients had BNF. The OCNF group had significantly higher inflammatory markers than the BNF group when diagnosis, but significantly lower clinical stages at the time and mortality as outcomes. CONCLUSION: We found that compared to BNF, OCNF can be detected at lower clinical stage by using indexes, such as NLR and LRINEC score, besides clinical findings, which may help contributing to patient's relief.

  2021, PloS one, 16(12) (12), e0260740, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of a miconazole oral patch on preventing development of oral candidiasis in patients with head and neck cancer undergoing radiotherapy: results of a preliminary study quantifying the prevalence of Candida albicans in saliva

  Ryuichiro Funahara, Sakiko Soutome, Madoka Funahara, Shoma Tsuda, Takumi Hasegawa, Masahiro Umeda, Masaya Akashi

  Purpose: Serious oral mucositis develops during radiation therapy (RT) for head and neck cancer, but there is no effective preventive method. We have used a steroid ointment to prevent oral mucositis during RT, but the use of steroid ointment is discontinued when oral candidiasis develops. Therefore, prevention of oral candidiasis is important. The purpose of this study was to examine whether administration of a miconazole oral patch reduced the amount of Candida albicans in saliva and prevented the development of oral candidiasis during RT. Methods: Participants were patients with head and neck cancer receiving RT ≥ 60 Gy. Patients in the intervention group received miconazole oral patches for 14 days after the appearance of grade 2 oral mucositis. The control group received oral care only. Total bacteria and C. albicans counts in the saliva were analyzed by real-time polymerase chain reaction. The incidence of oral candidiasis was compared between the groups. Results: Total bacterial counts did not change throughout RT in either the intervention or the control group. However, C. albicans count significantly increased at 30 Gy and 60 Gy in the control group but was suppressed in the intervention group. The saliva pH did not show a significant change throughout RT in either group. The incidence of oral candidiasis in the intervention group tended to be lower than that in the control group. Conclusion: This study suggested that prophylactic use of a miconazole oral patch was effective in suppressing the growth of C. albicans count in saliva during RT for head and neck cancer.

  2021, Supportive Care in Cancer

  Scientific journal


• Can four-dimensional computed tomography support diagnosis and treatment planning?: a case report before and after coronoidectomy.

  Wensu Huang, Masaya Akashi, Takuro Nishio, Noriyuki Negi, Akira Kimoto, Takumi Hasegawa

  INTRODUCTION: Elongated mandibular coronoid process is a rare condition defined as enlargement of the coronoid process. The only useful way to treat the mouth-opening limitation is resection of the elongated coronoid process. Four-dimensional CT (4DCT; three spatial dimensions plus time axis) is a novel imaging technique. Its usefulness of for evaluation of dynamic movements such as joint motion has been reported. Here, we show the potential usefulness of 4DCT evaluation in a patient with elongated mandibular coronoid process. CASE PRESENTATION: A 59-year-old female who suffered from mouth-opening difficulty and pressure during mouth opening was referred to our department. Elongation of the right coronoid process was evident in a panoramic X-ray image. The mandibular movement and temporal muscle motility before and after coronoidectomy in this patient on 4DCT could be evaluated. CONCLUSIONS: 4DCT is useful in the diagnosis and surgical outcome of elongated coronoid process.

  Corresponding, Dec. 2020, Oral and maxillofacial surgery, 24(4) (4), 515 - 520, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Does prophylactic antibiotic administration for tooth extraction affect PT-INR in patients taking warfarin?

  Eiji Iwata, Akira Tachibana, Junya Kusumoto, Naoki Takata, Takumi Hasegawa, Masaya Akashi

  BACKGROUND: Various antibiotics and analgesics have been reported to interact with warfarin. Reports that investigate the effects of medication taken for just a few days during tooth extraction on the prothrombin time-international normalized ratio are rare. METHODS: A total of 110 patients receiving long-term stable warfarin therapy underwent tooth extraction without interruption of warfarin treatment. INR values were measured 1 month before the tooth extraction, the day of the extraction, and 1 week after the extraction. We investigated the changes in INR values between the day of extraction and 1 week after extraction, as well as the various risk factors for increases in INR values. RESULTS: Before and after tooth extraction, the number of patients taking cefcapene pivoxil, amoxicillin, and azithromycin was 57, 36, and 8, respectively. Nine patients were administered ampicillin before tooth extraction and received amoxicillin after their tooth extraction. One week after tooth extraction, the INR values increased beyond the therapeutic range in 3 out of 110 patients (2.7%). The INR values before tooth extraction in these three patients were close to 3.0. The INR value increased by more than twice as much in 1 out of 110 patients (0.9%). CONCLUSION: Our results suggest that prophylactic antibiotic administration has little effect on INR values when patients on stable warfarin therapy undergo tooth extraction. Surgeons have to take attention if the patients whose INR values are close to 3.0 before their extraction.

  Nov. 2020, BMC oral health, 20(1) (1), 331 - 331, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 生体腎移植後患者に発症した歯性感染症によるガス壊疽の1例

  高田 直樹, 橘 進彰, 岩田 英治, 榎本 由依, 明石 昌也

  症例は68歳女性で、11年前に高血圧性腎硬化症を発症し、5年前に夫をドナーとする生体腎移植を受けていた。今回、左側下顎部の疼痛と腫脹を主訴に近在歯科医院を受診し、抗菌薬内服で症状改善を認めないため、精査加療目的に当科を紹介され救急受診した。画像所見より左側頭頸部ガス壊疽と診断し、側頭部および顎下部より切開排膿術、左側下顎第一小臼歯および左側下顎第一から第三大臼歯の抜歯を施行した。側頭筋、内側翼突筋、咬筋の壊死部はデブリードメントを行い、下顎骨の内外側で側頭部と顎下部をそれぞれ交通させペンローズドレーンを計3本留置した。また、初診時血液検査結果より急性腎不全が疑われたため、抗菌薬はスルバクタム・アンピシリン3g/日とクリンダマイシン1.2g/日を併用し経静脈投与した。切開部からの排膿はほぼ消失したため第27病日にすべてのドレーンを抜去し、第39病日に軽快退院となった。

  (一社)日本口腔感染症学会, Nov. 2020, 日本口腔感染症学会雑誌, 27(2) (2), 61 - 66, Japanese


• 生体腎移植後患者に発症した歯性感染症によるガス壊疽の1例

  高田 直樹, 橘 進彰, 岩田 英治, 榎本 由依, 明石 昌也

  (一社)日本口腔感染症学会, Nov. 2020, 日本口腔感染症学会雑誌, 27(2) (2), 61 - 66, Japanese


• 消化器外科SSI対策 経験からその検証へ 大腸癌術後SSIに対する周術期口腔ケアの予防効果に関する多施設共同研究

  延原 浩, 五月女 さき子, 林田 咲, 梅田 正博, 長谷川 巧実, 明石 昌也, 山田 慎一, 栗田 浩, 中原 寛和, 上田 順宏, 桐田 忠昭, 中村 知寿, 渋谷 恭之, 山口 泰平, 森 和代

  (一社)日本外科感染症学会, Oct. 2020, 日本外科感染症学会雑誌, 17(5) (5), 329 - 329, Japanese


• JCAD expression and localization in human blood endothelial cells.

  Manabu Shigeoka, Satomi Arimoto, Masaya Akashi

  Background: Junctional Cadherin 5 Associated (JCAD) is an endothelial, cell-cell junction protein, and its expression is associated with cardiovascular diseases including atherosclerosis and hypertension. However, to date, there are few studies confirming JCAD expression and precise localization in human tissues by immunohistochemical staining. Methods: JCAD expression and localization was assessed in four human submandibular gland (SMG) specimens by immunohistochemical staining. One specimen of SMG with sialoadenitis was accompanied by severe inflammation and fibrosis, while the other was largely normal. Other two SMGs were accompanied by severe fibrosis because of irradiation. Results: Immunohistochemical analysis of human SMGs revealed JCAD localization at the blood endothelial cell-cell junctions. JCAD expression was more evident in microvessels and arteries in areas affected by inflammation. Conclusions: The localization of JCAD at endothelial cell-cell junctions was confirmed in human tissues. JCAD expression may be affected by pathological conditions.

  Corresponding, Oct. 2020, Heliyon, 6(10) (10), e05121, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Phase I dose-escalation trial of S-1 combined with carbon-ion radiotherapy for sinonasal squamous cell carcinoma.

  Daiki Takahashi, Yusuke Demizu, Sung Chul Park, Yoshiro Matsuo, Nor Shazrina Sulaiman, Kazuki Terashima, Sunao Tokumaru, Masaya Akashi, Tomoaki Okimoto

  This study aimed to determine the maximum tolerance dose (MTD) and to estimate the recommended dose (RD) of concomitant S-1 with carbon-ion radiotherapy (RT) for sinonasal squamous cell carcinoma (SCC). Nine patients with sinonasal SCC received carbon-ion RT with escalating doses of S-1 according to phase I methods. Doses of 40, 60 and 80 mg/m2/day were administered twice daily in dose levels 1, 2 and 3, respectively, from days 1 to 14 and 22 to 35. Carbon-ion RT was administered at a dose of 70.4 Gy (relative biological effectiveness) in 32 fractions, 5 days a week. Two patients developed grade 3 acute dermatitis. However, none developed dose-limiting toxicities. Therefore, the MTD of S-1 could not be determined; the RD was estimated to be 80 mg/m2/day with concurrent carbon-ion RT. Partial response and stable disease were noted in 5 and 4 patients, respectively. The 2-year overall survival and local control rates were 56 and 74%, respectively. Overall, 2 patients developed ≥grade 3 late toxicities; among them, 1 patient developed grade 3 cataract and the other developed grade 4 cataract, optic nerve disorder and hearing impairment. To the best of our knowledge, this phase I study is the first clinical trial to evaluate concomitant S-1 with carbon-ion RT for sinonasal SCC. The MTD of S-1 could not be determined, and the RD was estimated to be 80 mg/m2/day. This study demonstrated a manageable safety profile for this combination. The observed outcomes may facilitate further evaluation of this novel therapy.

  Sep. 2020, Journal of radiation research, 61(5) (5), 733 - 739, English, International magazine

  Scientific journal


• 膵頭十二指腸切除術における周術期口腔機能管理の術後合併症予防効果

  山口 泰平, 高島 裕之, 森田 麻希, 渋谷 恭之, 五月女 さき子, 林田 咲, 村田 真穂, 梅田 正博, 延原 浩, 長谷川 巧実, 明石 昌也, 兒島 由佳, 小西 尚晃, 中原 寛和, 山田 慎一, 栗田 浩, 森 和代, 於保 孝彦

  (一社)日本口腔ケア学会, Sep. 2020, 日本口腔ケア学会雑誌, 14(3) (3), 114 - 114, Japanese


• 周術期口腔ケアによる胃癌術後合併症予防効果に関する臨床的検討

  高島 裕之, 五月女 さき子, 林田 咲, 村田 真穂, 梅田 正博, 延原 浩, 長谷川 巧実, 明石 昌也, 上田 順宏, 下辻 寛子, 桐田 忠昭, 小西 尚晃, 中原 寛和, 山田 慎一, 栗田 浩, 山口 泰平, 森 和代, 渋谷 恭之

  (一社)日本口腔ケア学会, Sep. 2020, 日本口腔ケア学会雑誌, 14(3) (3), 123 - 123, Japanese


• 口腔ケアのエビデンスを検証するためにはどのような研究をすればよいのか アカデミア臨床研究環境のこれまで 臨床研究法施行の背景

  筧 康正, 長谷川 巧実, 明石 昌也

  (一社)日本口腔ケア学会, Sep. 2020, 日本口腔ケア学会雑誌, 14(3) (3), 53 - 54, Japanese


• 大腸癌術後合併症に対する周術期口腔機能管理の予防効果に関する多施設共同研究

  延原 浩, 五月女 さき子, 林田 咲, 村田 真穂, 梅田 正博, 長谷川 巧実, 明石 昌也, 山田 慎一, 栗田 浩, 小西 尚晃, 中原 寛和, 上田 順宏, 下辻 寛子, 桐田 忠昭, 中村 知寿, 渋谷 恭之, 山口 泰平, 森 和代

  (一社)日本口腔ケア学会, Sep. 2020, 日本口腔ケア学会雑誌, 14(3) (3), 122 - 122, Japanese


• Clinical investigation on sarcoma in oral and maxillofacial regions: A multicenter retrospective analysis in Japan

  Ryuichi Itoh, Shin ichi Yamada, Takumi Hasegawa, Nobuhiro Yamakawa, Yuya Denda, Hiroki Otagiri, Masaya Okura, Souichi Yanamoto, Masaya Akashi, Tadaaki Kirita, Yoshihide Ota, Masahiro Umeda, Hiroshi Kurita

  The aim of the present study was to review the treatment outcomes of oral sarcoma in Japan based on a multicenter retrospective analysis. Between January 2001 and December 2015, 30 patients were histologically diagnosed with sarcoma in the oral and maxillofacial regions and retrospectively reviewed. Osteosarcoma was the most common in 6 (20 %) out of 30 cases, followed by 3 cases (10 %) of fibrosarcoma. The average maximum size of the primary tumor was 42.3 ± 18.7 mm (range: 5−80 mm). The mandible was the most common site of the primary tumor and observed in 15 cases (50 %), followed by the maxilla in 12 cases (40 %). Soft tissue sarcoma was noted in 3 cases (10 %). The average follow-up period was 5.6 ± 4.6 years (range, 0.1–14.7 years). Five- and 10-year OS rates were 81.4 and 62.0 %, respectively. Five- and 10-year OS rates in cases that underwent radical surgery were 95.5 and 72.7 %, respectively, and were significantly higher than those treated with non-surgical therapy (p < 0.0001). The early detection and complete surgical resection of primary tumors with adequate surgical margins appears to be important for improving the prognosis of oral sarcoma patients.

  Sep. 2020, Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, 32(5) (5), 356 - 359

  Scientific journal


• Intraepithelial Macrophage Expressing CD163 Is a Histopathological Clue to Evaluate the Malignant Potency of Oral Lichenoid Condition: A Case Report and Immunohistochemical Investigation.

  Manabu Shigeoka, Yu-Ichiro Koma, Maki Kanzawa, Masaya Akashi, Hiroshi Yokozaki

  Oral lichenoid conditions (OLC), including oral lichen planus (OLP), oral lichenoid lesions and oral lichenoid dysplasia, differ in pathogenesis and biological malignancy. However, distinguishing them based on clinical or histological features is difficult. It is well known that CD163+ macrophages are associated with oral cancer aggressiveness. We recently demonstrated that CD163+ macrophages of noncancerous lesions infiltrate the stroma, not the intraepithelial area. In this report, we describe a case of OLC that was not detected as malignant by the first local biopsy. Furthermore, we evaluated the malignant potency of OLC by retrospectively comparing the histological findings between local biopsy and resected specimens focusing on CD163+ macrophages. A 72-year-old man with a white lesion in the unilateral buccal mucosa was diagnosed with OLP through the biopsy although invasive cancer was detected two years later. Intraepithelial CD163+ macrophages were found not only on the resected specimen but also biopsy. This is the first report to demonstrate that intraepithelial CD163+ macrophages may be noteworthy indicators to identify the malignant potency of OLC.

  MDPI AG, Aug. 2020, Diagnostics (Basel, Switzerland), 10(9) (9), 624 - 624, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 全身麻酔導入時にロクロニウムによるアナフィラキシーをきたした1例

  高田 直樹, 橘 進彰, 高井 美玲, 榎本 由依, 岩田 英治, 黄 文蘇, 明石 昌也

  全身麻酔中のアナフィラキシーの原因は筋弛緩薬によるものが最も多い。今回われわれは、全身麻酔導入後にロクロニウムによるアナフィラキシーをきたした1例を経験したのでその概要を報告する。患者は41歳、女性。両側下顎智歯抜歯依頼にて当科紹介初診となった。全身麻酔下での両側下顎埋伏智歯抜歯術を予定した。プロポフォール、レミフェンタニル、ロクロニウムで導入を行ったが、59/32mmHgへ血圧が低下した。昇圧薬を投与したが血圧上昇を認めなかったため、アナフィラキシーが疑われた。皮膚症状も出現し、アドレナリン、ステロイド、クロルフェニラミンを投与した。バイタルサインが安定したため手術延期を決定し、抜管後ICU管理とした。二相性反応なく、バイタルサインは安定していたため翌日当科退院となった。79日後にアレルギーテストを行い、原因物質はロクロニウムとの診断であった。再手術時は筋弛緩薬を使用せず全身麻酔を行ったが、術中、術後とも問題なく順調に経過した。アナフィラキシーの原因物質を特定できたことで、アナフィラキシーを予防することが可能であった。治療に際しては医科関連各科と連携していくことが重要である。(著者抄録)

  (一社)日本有病者歯科医療学会, Aug. 2020, 有病者歯科医療, 29(3) (3), 75 - 80, Japanese


• Which symptoms negatively affect the oral health-related quality of life in patients with osteonecrosis of the jaw?

  Takumi Sato, Junya Kusumoto, Daisuke Takeda, Megumi Kishimoto, Masahiko Kashin, Shungo Furudoi, Masaya Akashi

  OBJECTIVES: One of the treatment goals for osteonecrotic lesions of the jaw, such as medication-related osteonecrosis of the jaw (MRONJ) or osteoradionecrosis (ORN), is restoration of quality of life (QOL). This study aimed to identify symptoms that negatively affect QOL in patients with unhealed MRONJ or ORN. STUDY DESIGN: This cross-sectional study included patients who were previously diagnosed with MRONJ or ORN and who underwent treatment at the Kobe University Hospital between June 2015 and February 2016. Patient QOL was measured by using the Oral Health Impact Profile (OHIP-14). The predictor variable was disease status (stage and healing). The outcome variable was OHIP-14. One-way analysis of variance and Tukey's test were performed. RESULTS: The study included 74 patients (37 men and 37 women; mean age 70 years). Although there was no significant difference between the OHIP-14 scores of unhealed MRONJ and ORN (stages 1-3) and those of healed ones, the "worsened sense of taste" resulted in significant differences among stages in patients with unhealed MRONJ (P = .027) and the "painful mouth aching" in patients with unhealed ORN (P = .041). CONCLUSIONS: Worsened sense of taste and pain negatively affected QOL in patients with unhealed MRONJ and ORN.

  Corresponding, Aug. 2020, Oral surgery, oral medicine, oral pathology and oral radiology, 130(2) (2), 175 - 180, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Postoperative adjuvant therapy for patients with loco-regionally advanced oral squamous cell carcinoma who are at high risk of recurrence

  S. Yanamoto, Y. Denda, Y. Ota, T. Hasegawa, M. Akashi, M. Okura, S. I. Yamada, H. Kurita, N. Yamakawa, T. Kirita, M. Ueda, M. Umeda

  Extranodal extension (ENE) of lymph node metastasis and the presence of a positive or close margin (PCM) are major risk factors for head and neck squamous cell carcinoma recurrence. This retrospective multicentre cohort study compared the prognostic impact of postoperative radiotherapy (RT) and concurrent chemoradiotherapy (CCRT) in oral squamous cell carcinoma (OSCC) patients at high risk of recurrence. One hundred and eighteen patients with PCM and/or ENE who underwent definitive surgery plus either adjuvant RT or CCRT using cisplatin for OSCC were investigated. The cohort-wide 5-year loco-regional control (LRC), disease-free survival (DFS), and overall survival (OS) rates (the main outcome measures) were 54.3%, 35.8%, and 43.2%, respectively. Multivariate analysis showed that age ≥64 years (hazard ratio (HR) 0.584), cT3–4 stage (HR 1.927), ≥4 metastatic lymph nodes (HR 1.912), and PCM (HR 2.014) were significant independent predictors of OS. Moreover, postoperative CCRT with cisplatin was associated with a significantly improved LRC rate, but not with improved DFS or OS rates, compared to postoperative RT (HR 0.360). Given that CCRT with cisplatin does not significantly improve survival, additional clinical trials will be required to validate new regimens that further improve the outcomes of patients with loco-regionally advanced OSCC going forward.

  Jul. 2020, International Journal of Oral and Maxillofacial Surgery, 49(7) (7), 848 - 853

  Scientific journal


• Prevalence of and risk factors for postoperative hemorrhage after lower third molar extraction on warfarin therapy: a multicenter retrospective study in Japan.

  Shin-Ichi Yamada, Takumi Hasegawa, Sakiko Soutome, Hitoshi Yoshimura, Masaaki Miyakoshi, Nobuhiro Ueda, Kenjiro Okamoto, Sumiyo Hishida, Satoshi Rokutanda, Hirokazu Nakahara, Shigeyuki Fujita, Masaya Akashi, Yoshimasa Kitagawa, Tadaaki Kirita, Yasuyuki Shibuya, Masahiro Umeda, Hiroshi Kurita

  Postoperative hemorrhage after tooth extraction is a critical and clinically important issue for clinicians and patients receiving anticoagulants. The purpose of the present study was to investigate the prevalence of and risk factors for postoperative hemorrhage after lower third molar extraction in Japanese patients receiving warfarin therapy. A total of 142 patients who underwent lower third molar extraction between January 2010 and December 2016 were included, and their medical records were retrospectively reviewed. The prevalence of and risk factors for postoperative hemorrhage were investigated. The prevalence of postoperative hemorrhage after lower third molar extraction was significantly higher in patients receiving warfarin than in healthy subjects (21.8% vs 0.7%, P < 0.001). The cutoff value for PT-INR was 2.11 based on a receiver-operating characteristic analysis. A multivariate analysis indicated that an elevated PT-INR value [hazard ratio (HR) 3.798, 95% confidence interval (CI) 1.400-10.467, P < 0.01], preoperative antibiotic administration (HR 4.434, 95% CI 1.591-14.775, P < 0.01), difficulties with intraoperative hemostasis (HR 16.298, 95% CI 2.986-110.677, P < 0.01), and higher serum creatinine levels (HR 7.465, 95% CI 1.616-39.576, P < 0.05) are significant predictors of postoperative hemorrhage after lower third molar extraction. Multivariate correlations were observed between risk factors including an elevated PT-INR value, preoperative antibiotic administration, and higher serum creatinine levels, and postoperative hemorrhage after lower third molar extraction in patients receiving warfarin therapy. Clinicians need to consider these risk factors for postoperative hemorrhage after the lower third molar extraction and monitor PT-INR in patients receiving warfarin therapy.

  Jul. 2020, Odontology, 108(3) (3), 462 - 469, English, Domestic magazine

  [Refereed]

  Scientific journal


• Neutrophil-lymphocyte ratio associated with poor prognosis in oral cancer: a retrospective study.

  Takumi Hasegawa, Tomoya Iga, Daisuke Takeda, Rika Amano, Izumi Saito, Yasumasa Kakei, Junya Kusumoto, Akira Kimoto, Akiko Sakakibara, Masaya Akashi

  BACKGROUND: Prognostic biomarkers provide essential information about a patient's overall outcome. However, existing biomarkers are limited in terms of either sample collection, such as requiring tissue specimens, or the process, such as prolonged time for analysis. In view of the need for convenient and non-invasive prognostic biomarkers for oral cancer, we aimed to investigate the prognostic values of neutrophil-to-lymphocyte ratio, lymphocyte-to-monocyte ratio, and platelet-to-lymphocyte ratio in patient survival. We also aimed to explore the associations of these ratios with the clinicopathologic characteristics of Japanese oral squamous cell carcinoma patients. METHODS: This study was a non-randomized retrospective cohort study in a tertiary referral center. We included 433 patients (246 men, 187 women) who underwent radical surgery for oral cancers between January 2001 and December 2013. We evaluated various risk factors for poor prognosis including neutrophil-to-lymphocyte ratio, lymphocyte-to-monocyte ratio, and platelet-to-lymphocyte ratio with univariate and multivariate analyses. The disease-specific survival and overall survival rates of patients were compared among the factors and biomarkers. RESULTS: In multivariable Cox proportional hazards analysis, high neutrophil-to-lymphocyte ratio (hazard ratio 2.87, 95% confidence interval 1.59-5.19, P <  0.001), moderately or poorly differentiated histology (hazard ratio 2.37, 95% confidence interval 1.32-4.25, P <  0.001), and extranodal extension (hazard ratio 1.95, 95% confidence interval 1.13-3.35, P = 0.016) were independent predictors of disease-specific survival. High neutrophil-to-lymphocyte ratio (hazard ratio 2.30, 95% confidence interval 1.42-3.72, P <  0.001), moderately or poorly differentiated (hazard ratio 1.72, 95% confidence interval 1.07-2.76, P = 0.025), and extranodal extension (hazard ratio 1.79, 95% confidence interval 1.13-2.84, P = 0.013) were independent predictors of overall survival. CONCLUSIONS: Neutrophil-to-lymphocyte ratio might be a potential independent prognostic factor in Japanese oral squamous cell carcinoma patients.

  Jun. 2020, BMC cancer, 20(1) (1), 568 - 568, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Clinical significance of the G8 screening tool in elderly patients with oral squamous cell carcinoma.

  Shin-Ichi Yamada, Takumi Hasegawa, Kohei Okuyama, Nobuhiro Yamakawa, Masaya Okura, Masao Hashidume, Souichi Yanamoto, Masaya Akashi, Tadaaki Kirita, Masahiro Umeda, Hiroshi Kurita

  OBJECTIVES: The present study investigated the clinical significance of the G8 screening tool in elderly oral squamous cell carcinoma (OSCC) patients using a multicenter analysis. MATERIALS AND METHODS: The medical records of 438 primary OSCC patients aged 75 years and older were retrospectively reviewed. To examine the clinical significance of the G8 screening tool in elderly OSCC patients, self-reliance (SR) rates and prognostic factors were statistically analyzed. RESULTS: The mean score of the G8 screening tool was 10.9 ± 2.93. The SR cut-off value of the G8 score was 9.5 in a receiver operating characteristic curve analysis. Five-year overall survival rates were 40.1% in patients with a G8 score < 9.5 and 58.4% in those with a G8 score ≥ 9.5 (p < 0.01). Five-year cancer-specific rates were 34.3% in patients with a G8 score < 9.5 and 55.7% in those with a G8 score ≥ 9.5 (p < 0.01). Five-year SR rates were 40.7% in patients with a G8 score < 9.5 and 55.3% in those with a G8 score ≥ 9.5 (p < 0.05). A multivariate analysis identified an advanced age (≥ 80 vs < 80; HR, 1.437; 95%CI, 1.181-1.843; p < 0.01), poorer ECOG-PS (2-4 vs 0-1; HR, 1.560; 95%CI, 1.14-2.106; p < 0.01), and standard treatment (non-standard therapy vs standard therapy; HR, 0.598; 95%CI, 0.405-0.910; p < 0.05) as significant independent prognostic factors for SR. A lower G8 score was associated with poorer SR (≥ 9.5 vs < 9.5; HR, 0.765; 95%CI, 0.575-1.034; p = 0.081). CONCLUSION: The clinical significance of the G8 score for elderly OSCC patients was demonstrated and its combination with ECOG-PS may be useful for assessing their prognoses.

  Jun. 2020, Clinical oral investigations, 24(6) (6), 1953 - 1961, English, International magazine

  [Refereed]

  Scientific journal


• Extent of neck dissection for patients with clinical N1 oral cancer.

  Yasumasa Kakei, Hirokazu Komatsu, Tsutomu Minamikawa, Takumi Hasegawa, Masanori Teshima, Hirotaka Shinomiya, Naoki Otsuki, Ken-Ichi Nibu, Masaya Akashi

  BACKGROUND: No clear consensus has been reached on the indication of supraomohyoid neck dissection (SOHND) for clinically positive lymph-node metastasis. PATIENTS: Consecutive 100 patients with previously untreated oral cancer treated at Kobe University Hospital were included in this study. All patients were clinically staged as anyTN1M0 and underwent radical dissection of the primary site and level I-V neck dissection as the initial treatment. RESULTS: None of the 100 patients had pathological lymph-node metastasis (pLN) to level V. pLN to level IV was observed in two patients with tongue cancer in whom clinical lymph-node metastasis was preoperatively observed at level II. CONCLUSIONS: Level V may be excluded in the neck dissection for patients with N1 oral cancers. Level IV dissection should be considered in the patient with tongue cancer and clinical lymph-node metastasis at level II.

  Jun. 2020, International journal of clinical oncology, 25(6) (6), 1067 - 1071, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Intraepithelial CD163+ macrophages in tongue leukoplakia biopsy: A promising tool for cancer screening.

  Manabu Shigeoka, Yu-Ichiro Koma, Takayuki Kodama, Mari Nishio, Masaya Akashi, Hiroshi Yokozaki

  OBJECTIVE: Oral leukoplakia has mixed and differing histopathological features, and it is thus difficult to reach an accurate histological diagnosis of oral leukoplakia based on a local biopsy alone. We recently demonstrated the significance of CD163+ macrophages in oral carcinogenesis. Herein we sought to determine whether CD163+ macrophages in biopsy specimens of oral leukoplakia help identify the overall histological nature of the lesion. PATIENTS AND METHODS: Twenty-six patients with tongue leukoplakia who underwent a histological examination by both a preoperative local biopsy and consecutive total excision were enrolled. We evaluated clinicopathological factors and the expression of CD163+ macrophages based on a retrospective comparison of the histological diagnostic concordance between the biopsies and excisions. RESULTS: Seventeen patients (diagnostic-agreement group) were diagnosed with squamous intraepithelial lesion based on both the biopsy and the excision. Nine patients (diagnostic-discrepancy group) were diagnosed with invasive cancer by excision, although invasive cancer was not observed in their biopsy specimens. Compared to the diagnostic-agreement group, the diagnostic-discrepancy group had more tongue leukoplakia with non-homogenous or high numbers of intraepithelial CD163+ macrophages. CONCLUSION: The evaluation of intraepithelial CD163+ macrophages in local biopsy specimens from tongue leukoplakia patients is a promising tool for cancer screening.

  Apr. 2020, Oral diseases, 26(3) (3), 527 - 536, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Factors associated with severe oral mucositis and candidiasis in patients undergoing radiotherapy for oral and oropharyngeal carcinomas: a retrospective multicenter study of 326 patients.

  Mika Nishii, Sakiko Soutome, Akiko Kawakita, Hirokazu Yutori, Eiji Iwata, Masaya Akashi, Takumi Hasegawa, Yuka Kojima, Madoka Funahara, Masahiro Umeda, Takahide Komori

  PURPOSE: The present retrospective multicenter study intended to investigate the factors associated with severe oral mucositis and candidiasis in patients undergoing radiotherapy for oral and oropharyngeal carcinomas. METHODS: A total of 326 patients who underwent radiotherapy for oral and oropharyngeal cancers were enrolled in the study. The patients' age, sex, body mass index, primary site, diabetes, serum albumin, creatinine, hemoglobin, leukocyte and lymphocyte, concurrent cisplatin or cetuximab, method of radiation, total radiation dose, feeding route, use of spacers, pilocarpine hydrochloride, and corticosteroid ointment were examined, and the associations of each variable with oral mucositis and candidiasis were analyzed by multivariate Cox regression analysis. RESULTS: Grade 3 oral mucositis occurred in 136 (41.7%) patients. Male sex, oropharyngeal cancer, low hemoglobin levels, low leukocytes or lymphocytes, concurrent cisplatin or cetuximab, and oral feeding were found to be significantly associated with a higher incidence of severe oral mucositis. Oral candidiasis occurred in 101 (31.0%) patients. Oropharyngeal cancer, low leukocyte count, and oral mucositis of grade 2 or higher were found to be significantly associated with a higher incidence of oral candidiasis. The use of a topical steroid ointment was not found to be a risk factor for oral candidiasis. CONCLUSIONS: The present retrospective study demonstrated that certain factors may predispose patients with oral and oropharyngeal cancers receiving radiotherapy to develop severe oral mucositis and oral candidiasis. A preventive strategy for severe oral mucositis needs to be established in the future for high-risk cases.

  Mar. 2020, Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer, 28(3) (3), 1069 - 1075, English, International magazine

  [Refereed]

  Scientific journal


• Tooth Loss Predicts Long-Term Prognosis of Esophageal Cancer After Esophagectomy.

  Susumu Miura, Tetsu Nakamura, Takumi Hasegawa, Yukiko Miura, Gosuke Takiguchi, Naoki Urakawa, Hiroshi Hasegawa, Masashi Yamamoto, Shingo Kanaji, Yoshiko Matsuda, Kimihiro Yamashita, Takeru Matsuda, Taro Oshikiri, Satoshi Suzuki, Masaya Akashi, Yoshihiro Kakeji

  BACKGROUND: Oral health is associated with various diseases, including cancer. Tooth loss is a simple and objective index of oral health. OBJECTIVE: The purpose of this study was to investigate the association between preoperative tooth loss and esophageal cancer prognosis after esophagectomy. METHODS: This study included 191 patients who underwent esophagectomy for esophageal cancer after perioperative dental evaluation and oral care at Kobe University Hospital from April 2011 to March 2016. Patients were divided into two groups: Group A (tooth loss < 7) and Group B (tooth loss ≥ 7). Three-year overall survival (OS) and multivariate analysis were performed, along with subgroup analysis for elderly patients (age ≥ 65 years). RESULTS: The 3-year OS rate was 68.1% in Group A (104 patients) and 49.2% in Group B (87 patients). Group A had significantly higher OS than Group B (p = 0.002), and there were no significant differences in sex and clinical T or N stage between the two groups. However, the mean age of Group A was younger than that of Group B (64.2 vs. 68.5 years; p = 0.0002). Among elderly patients, the 3-year OS rate was 68.2% in Group A (55 patients) and 45.1% in Group B (65 patients) [p = 0.003]. Multivariate analysis that included age demonstrated that tooth loss is an independent prognostic factor (hazard ratio 1.87, 95% confidence interval 1.22-2.87), in addition to clinical T stage and preoperative serum albumin. CONCLUSION: Tooth loss is an independent prognostic factor for esophageal cancer after esophagectomy.

  Mar. 2020, Annals of surgical oncology, 27(3) (3), 683 - 690, English, International magazine

  [Refereed]

  Scientific journal


• CD163+ Foamy Macrophages Are Associated with the Morphogenesis of Oral Verruciform Xanthoma through Angiogenesis by VEGF Expression: An Immunohistochemical Study.

  Manabu Shigeoka, Yu-Ichiro Koma, Takayuki Kodama, Mari Nishio, Masaya Akashi, Hiroshi Yokozaki

  Oral verruciform xanthoma (OVX) is an uncommon benign lesion that is characterized histologically by the accumulation of several foamy macrophages in the lamina propria papillae. The pathogenesis of OVX has not been completely elucidated, although the significance of macrophage polarization (M1, tumor suppression; and M2, tumor promotion) and the contribution of M2 macrophages to angiogenesis are well established. This study investigated the role of foamy macrophages in OVX, with a focus on angiogenesis. Four patients who underwent surgical excision or total excisional biopsy for OVXs were enrolled in this study. We evaluated the expression of the macrophage markers CD68 (broad) and CD163 (M2) and the CD34-positive microvessel density (MVD) of OVXs. The foamy macrophages of all patients exhibited positivity to CD68 and CD163. We evaluated the MVD and the expression of the vascular endothelial growth factor (VEGF) based on histological architecture. The MVD of all OVX cases was significantly higher than that of the corresponding normal epithelia. Interestingly, the MVD of verrucous-type OVX cases was higher than that of the other type. VEGF was expressed on foamy macrophages in all cases. Overall, the foamy macrophages expressing CD163 were associated with the morphogenesis of OVX through the process of angiogenesis by VEGF expression.

  Feb. 2020, Dentistry journal, 8(1) (1), English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Gold Nanoparticles Enhance EGFR Inhibition and Irradiation Effects in Head and Neck Squamous Carcinoma Cells.

  Masahiko Kashin, Yasumasa Kakei, Shun Teraoka, Takumi Hasegawa, Akinobu Yamaguchi, Takao Fukuoka, Ryohei Sasaki, Masaya Akashi

  Cetuximab, an epidermal growth factor receptor inhibitor (EI), is currently the only targeted molecular therapy used in combination with radiotherapy for head and neck squamous cell carcinoma (HNSCC). Gold nanoparticles (AuNPs) are expected to enhance radiotherapy effects in cancers. To investigate whether AuNPs combined with AG1478, an EI, enhanced irradiation effects on HNSCC cells, we first examined AG1478 adsorption on AuNP surfaces, using surface-enhanced Raman scattering, which indicated an adsorption equilibrium of AG1478 to AuNPs. We then used transmission electron microscopy to find internalization rates of AuNP alone and AuNP+AG1478; we found that intracellular uptake of AuNP alone and AuNP+AG1478 did not significantly differ. We compared cell numbers, proliferation, apoptosis, and migration between control cells and those treated with or without 60 nm AuNP (1.0 nM), AG1478 (0.5 μM), and irradiation (4 Gy). We found that AuNP+AG1478 inhibited proliferation more than AG1478 alone; the combination of irradiation+AuNP+AG1478 significantly reduced total cell numbers compared with the combination of irradiation+AuNP; AuNP+AG1478 increased apoptotic reaction to irradiation; the combinations of AuNP+AG1478 and irradiation+AuNP induced more apoptosis than AG1478+irradiation. Whereas AuNP+AG1478 enhanced cytotoxicity in human HNSCC cells by inhibiting proliferation, irradiation+AuNP enhanced cytotoxicity by inducing apoptosis.

  2020, BioMed research international, 2020, 1281645 - 1281645, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effect of low-intensity pulsed ultrasound after intraoral vertical ramus osteotomy.

  Satomi Arimoto, Takumi Hasegawa, Daisuke Takeda, Chizu Tateishi, Masaya Akashi, Shungo Furudoi, Takahide Komori

  OBJECTIVE: The present study investigated the effect of low-intensity pulsed ultrasound (LIPUS) on long-term osseous healing of the cleavage space between bone fragments after intraoral vertical ramus osteotomy (IVRO). STUDY DESIGN: Patients undergoing IVRO were randomly assigned to the LIPUS group (n = 12) or the control group (n = 9) after surgery. LIPUS treatments were applied daily to the cleavage space between bone fragments for 3 weeks. We observed 3-dimensional quantitative color mapping of the whole mandible created by computed tomography (CT) data at 1 month, 6 months, and 1 year postoperatively. On the basis of CT values, the color grades were classified as D1 to D5 by using the Misch criteria. We then calculated mean CT values and rated each color grade in different selection ranges. RESULTS: The mean CT values of the LIPUS group were significantly higher than those of the control group at 1 month, 6 months and 1 year postoperatively (P < .01). The color grades of the cleavage between bone fragments increased from D5 to D1 over time. CONCLUSIONS: Our results indicated that LIPUS promoted osseous healing after IVRO, thus improving bone density and offering clinical benefits.

  Dec. 2019, Oral surgery, oral medicine, oral pathology and oral radiology, 128(6) (6), 581 - 589, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 「口腔がん治療における周術期口腔機能管理とは?」 口腔・中咽頭がん放射線治療時の口腔粘膜炎と口腔カンジダ症のリスク因子 多施設共同研究による326例の解析結果

  西井 美佳, 五月女 さき子, 長谷川 巧実, 柚鳥 宏和, 川北 晃子, 兒島 由佳, 船原 まどか, 梅田 正博, 明石 昌也, 古森 孝英

  口腔・中咽頭がん放射線治療患者326例の口腔粘膜炎と口腔カンジダ症について後ろ向きに検討し、以下の結果を得た。1.グレード3口腔粘膜炎は、326例中131例(41.7%)に生じた。2.グレード3口腔粘膜炎の発症リスク因子として、男性、中咽頭原発、シスプラチンやセツキシマブ併用、白血球低値・リンパ球低値・ヘモグロビン低値、経口摂取が挙げられた。3.口腔カンジダ症は、326例中101例(31.0%)に生じた。4.口腔カンジダ症発症のリスク因子として、中咽頭原発、白血球減少、グレード2以上の口腔粘膜炎が挙げられた。ステロイド軟膏はリスク因子ではなかった。(著者抄録)

  (一社)日本口腔腫瘍学会, Sep. 2019, 日本口腔腫瘍学会誌, 31(3) (3), 111 - 116, Japanese

  [Refereed]


• [総説]口腔・中咽頭がん患者の放射線性顎骨壊死発症リスク因子について：多施設共同後ろ向き研究の結果より

  兒島由桂, 河岡有美, 川下由美子, 山田慎一, 栗田 浩, 長谷川巧実, 古森孝英, 明石昌也, 上田順宏, 桐田忠昭, 高島裕之, 渋谷恭之, 梅田正博

  2008年〜2017年に6施設で放射線治療を行った口腔・中咽頭がん患者366例において、放射線顎骨壊死(ORN)の発症リスク因子をCox比例ハザードモデルにて解析した。その結果、根尖病巣、重度歯周病、血清クレアチニン上昇がORN発症リスクを有意に増大させ、IMRT、照射前抜歯がリスクを有意に低下させた。照射後抜歯は有意ではなかったがリスクを増加させた。今後、ORNの発症予防法を確立する必要がある。(著者抄録)

  (一社)日本口腔腫瘍学会, Sep. 2019, 日本口腔腫瘍学会誌, 31(3) (3), 117 - 120, Japanese


• Dental intervention against osteoradionecrosis of the jaws in irradiated patients with head and neck malignancy: a single-arm prospective study.

  Yumi Muraki, Masaya Akashi, Yasuo Ejima, Takumi Hasegawa, Daisuke Miyawaki, Hirotaka Shinomiya, Mika Nishii, Naoki Otsuki, Ryohei Sasaki, Ken-Ichi Nibu, Takahide Komori

  BACKGROUND: The purpose of this study was to investigate the effectiveness of dental intervention before and after radiation therapy (RT) for head and neck malignancy on prevention of osteoradionecrosis (ORN) of the jaws. METHODS: This is a single-arm prospective study according to intervention protocol of prophylactic dental extraction before RT and routine follow-up after RT. The primary endpoint was the occurrence of jawbone exposure during the first 2 years after RT. RESULTS: Sixty-seven patients were assessed. Before RT, 144 teeth among 39 patients (58%) were prophylactically extracted. The occurrence of transient jawbone exposure during the first 2 years after RT was 7%. Because those jawbone exposures healed with intervention after RT, no jawbone exposure was found at 2 years after RT. CONCLUSIONS: Dental intervention both before and after RT seemed to be important to prevent ORN development. Further studies in larger cohorts are necessary.

  Corresponding, Sep. 2019, Oral and maxillofacial surgery, 23(3) (3), 297 - 305, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effect of size difference between hemiglossectomy and reconstruction flap on oral functions: A retrospective cohort study.

  Akiko Sakakibara, Junya Kusumoto, Shunsuke Sakakibara, Takumi Hasegawa, Masaya Akashi, Tsutomu Minamikawa, Shungo Furudoi, Kazunobu Hashikawa, Takahide Komori

  BACKGROUND AND OBJECTIVE: Forearm free flaps are used after hemiglossectomy. However, no investigation has been performed on whether oral functions are better preserved when sizes of the resection and reconstruction flap are exact matches, or whether the size of the resection should be changed. We aimed to retrospectively examine whether size differences between the resection and reconstruction flap affect speech and swallowing functions postoperatively, and to determine whether there are more favorable flap size ratios. METHODS: This is a retrospective cohort study of patients undergoing hemiglossectomy using a forearm free flap between 2006 and 2016 at Kobe University Hospital, Japan. The effect of size difference between the resection and reconstruction flap on maintained oral function was assessed. Speech and swallowing functions were assessed, and their correlation with the ratio of the flap size to that of the resected area was determined. With these data, distribution maps of the relationship between the functional level and reconstructed dimension ratio were prepared. The more suitable reconstructed dimension ratio was examined and evaluated. The Fisher exact test, Kruskal-Wallis test, and Scheffe test were used in statistical analyses. RESULTS: Eighty-eight patients underwent hemiglossectomy using a forearm free flap during a 10-year period. Of these cases, 66 patients were included in this study, while 22 were excluded. The ratio of the area of the reconstruction flap to that of the resection site was 0.59-2.79 (median: 1.61). Sixty patients had flaps greater than the resection area, whereas 6 had smaller flaps. Significant differences were found in speech intelligibility and swallowing function when the reconstructed dimension ratio was categorized as follows: ≤1.3, 1.3-1.8, and ≥1.8. CONCLUSION: Our findings suggest that postoperative deterioration of oral functions after hemiglossectomy could be reduced if reconstruction is performed using a forearm free flap with a surface area 1.3 to 1.8 times greater than that of the resection area.

  Jul. 2019, Journal of plastic, reconstructive & aesthetic surgery : JPRAS, 72(7) (7), 1135 - 1141, English, International magazine

  Scientific journal


• Dental Rehabilitation for Free Fibula Flap-Reconstructed Mandible with Scar Contracture: A Technical Note.

  Masaya Akashi, Kousuke Matsumoto, Daisuke Takeda, Junya Yamashita, Nanae Yatagai, Kazunobu Hashikawa, Takahide Komori

  Dental rehabilitation with osseointegrated implants in reconstructed mandibles is a common procedure, but the technique still requires improvement, especially in its reliability and technical simplification. We herein report dental rehabilitation of a free fibula-reconstructed mandible with scar contracture. A vestibuloplasty technique with application of a polyglycolic acid (PGA) sheet is described. The implants were inserted into a viable fibula flap with severe scar contracture of the overlying epithelium resulting from vascular instability in skin paddle. Only the fibula periosteum was sutured after implant insertion; exposed surfaces were covered with a combination of PGA sheet and fibrin sealant. The area with PGA sheet coverage gradually healed with moderate contracture. The epithelium around the almost implants became immobilized. The implant-supported removable partial denture with custom titanium bar was acceptable. Dental rehabilitation is possible for reconstructed mandibles with severe scar contracture. Application of a PGA sheet may be useful for vestibuloplasty in patients with reconstructed mandibles.

  Jun. 2019, Dentistry journal, 7(3) (3), English, International magazine

  [Refereed]

  Link to Kobe Univ. Repository (Kernel)


• A radiologic evaluation of the incidence and morphology of maxillary sinus septa in Japanese dentate maxillae.

  Daisuke Takeda, Takumi Hasegawa, Izumi Saito, Satomi Arimoto, Masaya Akashi, Takahide Komori

  PURPOSE: Evaluation of the inner aspect of the maxillary sinus is important for the success of a sinus lift procedure. The most common surgical complication is perforation of the Schneiderian membrane, which is thought to occur because of the presence of maxillary sinus septa. Therefore, we retrospectively investigated the incidence and morphology of maxillary sinus septa using multiplanar reformatted computed tomographic (CT) images from dentate Japanese patients. METHODS: A total of 276 patients and 552 maxillary sinus segments were evaluated. The maxillary sinus septa were divided into four locations: forefront, anterior, middle, and posterior. The heights of the septa were measured at three sites from the deepest point of the sinus floor: lateral, mid-point, and medial. RESULTS: Sinus septa were identified in 191 of 552 (34.6%) maxillary sinus segments obtained from 111 of 276 (40.2%) patients. One unilateral septum was most commonly detected, and the sinus septa were most often located in the middle of the maxillary sinus. The average height of the identified septa was 8.69 ± 4.68 mm (mean ± standard deviation). CONCLUSION: Multiplanar reformatted CT images can identify maxillary sinus septa in any plane. The height of maxillary sinus septa in the dentate maxillae was higher than detected in previous studies. Appropriate treatment planning using CT images should be considered to prevent surgical complications.

  Jun. 2019, Oral and maxillofacial surgery, 23(2) (2), 233 - 237, English, International magazine

  Scientific journal


• Long-Term Effects on Volume Change in Musculocutaneous Flaps after Head and Neck Reconstruction.

  Akiko Sakakibara, Junya Kusumoto, Shunsuke Sakakibara, Takumi Hasegawa, Masaya Akashi, Tsutomu Minamikawa, Shungo Furudoi, Kazunobu Hashikawa, Takahide Komori

  OBJECTIVE:  Musculocutaneous flap reconstruction surgery is one of the standard procedures following head and neck cancer resection. However, no previous studies have classified flaps in terms of muscle and fat or examined them after long-term follow-up. The purpose of this study was to estimate the fat and muscle volume changes in musculocutaneous flaps during long-term follow-up. METHODS:  We conducted a retrospective analysis of 35 patients after musculocutaneous flap reconstruction. The total, fat, and muscle volumes of the musculocutaneous flaps were measured using 3-dimensional images. Changes in flap volumes over time (1 month, 1 year [POY1], and 5 years [POY5] postoperatively) were assessed. Flap persistence was calculated using flap volumes at 1 month after reconstruction for reference. RESULTS:  Flap persistence at POY5 was 42.0% in total, 64.1% in fat, and 25.4% in muscle. Muscle persistence was significantly decreased (p < 0.0001). In a multiple regression analysis, decreased body mass index (BMI) of ≥ 5% influenced fat persistence less than muscle persistence at POY1; however, there was no significant difference at POY5. Postoperative radiation therapy was associated with a significant decrease in total flap persistence at POY1 (p = 0.046) and POY5 (p = 0.0097). Muscle persistence significantly decreased at POY5 (p = 0.0108). Age significantly influenced muscle volume at POY1 (p = 0.0072). CONCLUSION:  Reconstruction flaps are well-preserved with high fat-to-muscle ratios. Recommendations for weight maintenance are necessary for patients less than 2 years after surgery due to the influence of BMI on fat persistence. Radiation therapy is necessary for some patients based on their disease state. Intensity-modulated radiation therapy can be offered to reduce scattering irradiation to normal tissues.

  May 2019, Journal of reconstructive microsurgery, 35(4) (4), 235 - 243, English, International magazine

  [Refereed]

  Scientific journal


• Induced Pluripotent Stem Cell-related Genes Correlate With Poor Prognoses of Oral Squamous Cell Carcinoma.

  Yumi Muraki, Takumi Hasegawa, Daisuke Takeda, Takeshi Ueha, Eiji Iwata, Izumi Saito, Rika Amano, Akiko Sakakibara, Masaya Akashi, Takahide Komori

  BACKGROUND/AIM: We recently investigated the contribution of the iPS-related genes SOX2, OCT4, and Nanog to de-differentiation by assaying for their mRNA levels. Given that mRNA expression does not always correlate with the protein levels, the aim of this study was to retrospectively determine the expression of these four iPS-related factors in human OSCC specimens by immunohistochemistry and examine their association with patient prognosis. MATERIALS AND METHODS: iPS cell-related gene expression in 89 OSCC patients by tissue microarray, and its correlation with clinicopathological factors, differentiation, metastasis, and poor prognoses were investigated. RESULTS: No evidence of statistically significant relationships was found between the expression of iPS cell-related genes and clinicopathological parameters. However, our data indicated that KLF4 expression was associated with survival, and poor tumor differentiation. In addition, high expression of KLF4 was an independent poor prognostic factor (p=0.004) for OSCC patients. CONCLUSION: In preoperative biopsies, higher KLF4 and poor differentiation may be clinically effective predictors for the prognosis of oral cancer.

  Mar. 2019, Anticancer research, 39(3) (3), 1205 - 1216, English, International magazine

  [Refereed]

  Scientific journal


• Perioperative intervention by oral medicine team in cardiovascular surgery patients.

  Masaya Akashi, Nagisa Nanba, Junya Kusumoto, Takahide Komori

  In brief, perioperative oral intervention consists of elimination of odontogenic foci and maintenance of oral hygiene in patients undergoing surgery. The importance of oral intervention before, during, and after medical treatments is well-known, especially in cancer patients, because odontogenic foci such as untreated deep dental caries or periodontitis can cause systemic infection in patients with myelosuppression resulting from chemotherapy. Although perioperative oral intervention is currently recommended for patients with cardiovascular disease, its efficacy in this population has not been established. This article consists of three sections: first, we review the current knowledge about the association between dental disease and cardiovascular disease to show the importance of oral hygiene maintenance and the risks of invasive dental procedures in patients with cardiovascular disease; second, we introduce pertinent, but limited evidence concerning the effect of oral care in preventing postoperative pneumonia; and finally, we present the optimal strategy for perioperative oral intervention in cardiovascular surgery patients.

  Feb. 2019, General thoracic and cardiovascular surgery, 67(2) (2), 197 - 202, English, Domestic magazine

  [Refereed][Invited]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A literature review of perioperative antibiotic administration in surgery for medication-related osteonecrosis of the jaw.

  Masaya Akashi, Junya Kusumoto, Daisuke Takeda, Takashi Shigeta, Takumi Hasegawa, Takahide Komori

  PURPOSE: Few studies exist that focus on the details of perioperative antibiotic administration for surgery to treat medication-related osteonecrosis of the jaw (MRONJ). The regime and duration of perioperative antibiotics applied in published studies were reviewed to clarify appropriate perioperative antibiotic use in MRONJ surgery. METHODS: A literature search was conducted using the MEDLINE database via PubMed. RESULTS: The search resulted in 453 hits on PubMed. After reading the downloaded full-text articles, 17 articles met the inclusion and exclusion criteria. The most common perioperative antibiotic used for MRONJ surgery was a combination of penicillin-based antibiotics and β-lactamase inhibitor (52.9%), and the second most common regime was penicillin-based antibiotics with metronidazole (17.6%). The duration of administration was 2 weeks postoperatively in nine studies, whereas four studies applied long-term administration (2-6 weeks postoperatively). CONCLUSIONS: Oral and maxillofacial surgeons mostly prefer penicillin-based antibiotics plus β-lactamase inhibitor or metronidazole for MRONJ surgery. The duration of administration of these medications may be based on empirical experience.

  Dec. 2018, Oral and maxillofacial surgery, 22(4) (4), 369 - 378, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Lymphangiogenesis and Lymph Node Metastasis in Oral Squamous Cell Carcinoma.

  Satomi Arimoto, Takumi Hasegawa, Daisuke Takeda, Izumi Saito, Rika Amano, Masaya Akashi, Takahide Komori

  BACKGROUND/AIM: Tumor lymphangiogenesis plays a key role in lymph node (LN) metastasis in oral squamous cell carcinoma (OSCC). The purpose of this study was to investigate podoplanin and lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) and their relationship to nodal metastasis and other clinicopathological variables. PATIENTS AND METHODS: Podoplanin and LYVE-1 expression of the primary tumor and normal tissue were investigated by means of a quantitative real-time PCR assay and immunohistochemistry in samples from 33 cases of OSCC. RESULTS: The mRNA high expression levels of both genes had a statistically significantly higher rate of LN metastasis (p<0.01) and histological grade (p<0.01 for podoplanin, p<0.05 for LYVE-1). High expression of each gene, as shown by immunohistochemistry, had a statistically significant higher rate of LN metastasis (p<0.01 for podoplanin, p<0.05 for LYVE-1). CONCLUSION: Podoplanin and LYVE-1 were strongly associated with LN metastasis.

  Nov. 2018, Anticancer research, 38(11) (11), 6157 - 6162, English, International magazine

  [Refereed]

  Scientific journal


• Gold nanoparticles enhance X-ray irradiation-induced apoptosis in head and neck squamous cell carcinoma in vitro.

  Shun Teraoka, Yasumasa Kakei, Masaya Akashi, Eiji Iwata, Takumi Hasegawa, Daisuke Miyawaki, Ryohei Sasaki, Takahide Komori

  Enhancing the antitumor effect of radiation, while reducing damage to organs, is a significant challenge in radiation therapy for head and neck malignancies. One promising radiosensitizer is gold. The present study aimed to determine whether gold nanoparticles (AuNPs) have the potential to enhance the effects of X-ray irradiation on head and neck cancer cells. The human head and neck carcinoma cell line HSC-3 was used. Total cell number and the levels of cell proliferation and apoptosis were compared between control cells and cells treated with 5-nm AuNPs alone at four concentrations (0.1, 0.4, 1.0 and 10.0 nM), X-ray irradiation alone at three doses (2, 4 and 8 Gy), or a combination of 4 Gy X-ray irradiation and 1.0 nM AuNPs. Analysis of variance and Tukey-Kramer testing were performed to compare the different groups. The total number of cells significantly decreased following 4 and 8 Gy X-ray irradiation, compared with in the control group (control vs. 4Gy, P=2.19×10-4; control vs. 8Gy, P=1.28×10-6). The combination of 4 Gy X-ray irradiation and 1.0 nM AuNPs significantly reduced the total number of cells compared with 4 Gy X-ray irradiation alone (P=2.95×10-4). Cell proliferation was not affected by AuNP treatment alone, 4 Gy X-ray irradiation alone or the combination of X-ray irradiation and AuNPs. The combination of 4 Gy irradiation and 1.0 nM AuNPs significantly increased the number of apoptotic cells compared with 4 Gy irradiation alone (P=0.0261). In conclusion, AuNPs combined with X-ray irradiation enhanced the cytotoxic effect on human head and neck cancer cells in vitro, through the induction of apoptosis, but not inhibition of cell proliferation.

  Corresponding, Nov. 2018, Biomedical reports, 9(5) (5), 415 - 420, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effect of local application of transcutaneous carbon dioxide on survival of random-pattern skin flaps.

  Izumi Saito, Takumi Hasegawa, Takeshi Ueha, Daisuke Takeda, Eiji Iwata, Satomi Arimoto, Akiko Sakakibara, Masaya Akashi, Shunsuke Sakakibara, Yoshitada Sakai, Hiroto Terashi, Takahide Komori

  INTRODUCTION: Skin flap procedures are widely used to reconstruct skin and soft tissue defects. Skin flap necrosis is a serious postoperative complication. Many researchers have introduced pharmacological agents to improve flap ischemia in experimental studies. However, outcomes of these studies remain controversial. We previously demonstrated that transcutaneous CO2 application improves hypoxia in fracture repair. In this study, we hypothesized that improving hypoxia by transcutaneous CO2 application can improve the blood flow in skin flaps and increase angiogenesis. We investigated whether transcutaneous CO2 application can increase the survival of random-pattern skin flaps. MATERIALS AND METHODS: Six-week-old male Sprague-Dawley rats were divided into two equal groups: the control group (n = 6) and CO2 group (n = 6). A random-pattern skin flap was constructed in these rats. Topical CO2 was applied using a hydrogel every day for 5 days in the CO2 group. The flap survival area was measured on postoperative days 1, 3, and 5. The vessel density and expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and hypoxia-inducible factor-1α (HIF-1α) were evaluated on postoperative day 5. RESULTS: A statistically significant difference was found in the percentage of the flap survival area between the two groups on postoperative days 3 and 5 (p < 0.05). Furthermore, the expression of VEGF and bFGF was significantly higher and that of HIF-1α was significantly lower in the CO2 than in the control group (p < 0.05). CONCLUSIONS: Transcutaneous CO2 application can improve the blood flow in skin flaps and increase angiogenesis, thus increasing the survival of random-pattern skin flaps.

  Nov. 2018, Journal of plastic, reconstructive & aesthetic surgery : JPRAS, 71(11) (11), 1644 - 1651, English, International magazine

  [Refereed]

  Scientific journal


• Delayed Socket Healing After Dental Extraction in Patients Undergoing Myelosuppressive Chemotherapy for Hematological Malignancy: Incidence and Risk Factors.

  Masaya Akashi, Megumi Kishimoto, Junya Kusumoto, Kimikazu Yakushijin, Hiroshi Matsuoka, Takahide Komori

  PURPOSE: The purpose of this study was to measure the frequency and identify factors associated with delayed socket healing after dental extraction in patients undergoing myelosuppressive chemotherapy for hematologic malignancy. MATERIALS AND METHODS: This prospective cohort study focused on delayed healing after extraction in patients with hematologic malignancy. Sockets with delayed healing were defined as those with intense pain and bone exposure 1 week postoperatively. Patients with and without delayed socket healing were compared using the Fisher exact test and Mann-Whitney U test with some variables. Receiver operating characteristics curve analysis was conducted to define cutoff values for delayed healing. RESULTS: One hundred ninety-four dental extractions in 93 patients (median age, 64 yr; range, 20 to 85 yr) were analyzed. The incidence of delayed socket healing was 7.5% (7 of 93 patients). There was no postoperative bleeding. Older age, type of hematologic malignancy (acute leukemia), shorter time from dental extraction to initiation of chemotherapy, low platelet count or hemoglobin level, requirement for red blood cell concentrate or platelet transfusion, and use of an absorbable hemostatic agent were statistically associated with the occurrence of delayed socket healing. Platelet and hemoglobin cutoffs were 4.6 × 104/μL and 7.7 g/dL, respectively. CONCLUSIONS: Although dental extraction can be safely performed in patients undergoing myelosuppressive chemotherapy for hematologic malignancy, oral surgeons should understand the potential risk for delayed socket healing. When considering dental extraction, patients with hematologic malignancy and low hemoglobin or platelet levels should be informed about the possibility of delayed socket healing.

  Oct. 2018, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 76(10) (10), 2057 - 2065, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Association between pain severity and clinicohistopathologic findings in the mandibular canal and inferior alveolar nerve of patients with advanced mandibular osteoradionecrosis.

  Yujiro Hiraoka, Masaya Akashi, Satoshi Wanifuchi, Junya Kusumoto, Manabu Shigeoka, Takumi Hasegawa, Kazunobu Hashikawa, Hiroto Terashi, Takahide Komori

  OBJECTIVE: Pain is one of the most problematic symptoms in patients with osteoradionecrosis of the jaws. This study investigated the associations between pain severity and morphologic alterations of the mandibular canal and inferior alveolar nerve, in respective computerized tomography images and resected specimens of mandibular osteoradionecrosis. STUDY DESIGN: We assessed 14 lesions in 13 patients who underwent segmental mandibulectomy for surgical debridement and simultaneous reconstruction with free fibula flap (1 patient exhibited bilateral lesions). The extent of the mandibular canal bone defect on preoperative coronal computerized tomography images and the number of inferior alveolar nerve fascicles in resected specimens were evaluated. Comparisons were made between the slight pain and extreme pain groups. In most of the patients in the extreme pain group, either mandibular canal bone defects were absent or entire circumferential defects were present; inferior alveolar nerve fascicles were either distinguishable or completely absent in the resected specimens. RESULTS: Although there was no statistically significant association between extreme pain and computerized tomography or histopathologic findings, the histopathologically indistinguishable inferior alveolar nerve fascicles was significantly associated with slight pain. CONCLUSIONS: The degree of degeneration of mandibular canal and inferior alveolar nerve may be associated with pain severity in patients with mandibular osteoradionecrosis.

  Corresponding, Mosby Inc., Sep. 2018, Oral surgery, oral medicine, oral pathology and oral radiology, 126(3) (3), 264 - 271, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Ionizing Radiation Enhances Paracellular Permeability Through Alteration of Intercellular Junctions in Cultured Human Lymphatic Endothelial Cells.

  Megumi Kishimoto, Masaya Akashi, Yasumasa Kakei, Junya Kusumoto, Akiko Sakakibara, Takumi Hasegawa, Shungo Furudoi, Ryohei Sasaki, Takahide Komori

  BACKGROUND: A problematic complication after radiation therapy is lymphedema. Development of lymphedema is associated with an increase in lymphatic paracellular permeability. The current study investigated the effects of radiation on intercellular junctions and paracellular permeability in cultured human dermal lymphatic endothelial cells (HDLECs). METHODS AND RESULTS: Double immunofluorescence staining with vascular endothelial (VE)-cadherin and actin immediately after X-ray irradiation (5 or 20 Gy) was performed. Morphological changes induced by irradiation were assessed. Cell viability and paracellular permeability after irradiation were also evaluated. Broad junctions in which VE-cadherin was accumulated at cell-cell contacts and almost colocalized with actin were significantly decreased in a dose-dependent manner in confluent and sparse irradiated HDLECs. Irradiation shortened the width of VE-cadherin-positive areas at the cell-cell contacts. Actin filaments did not colocalize with VE-cadherin after 20 Gy irradiation. Although cell viability was not affected by irradiation, paracellular permeability significantly increased in a dose-dependent manner. CONCLUSIONS: A dose of 5 or 20 Gy irradiation in HDLECs does not affect cell viability, but changes VE-cadherin mediated intercellular junctions and actin structure, resulting in an increase of paracellular permeability. Further investigations on the regulatory proteins involved in radiation-induced changes, which were observed in the current study, may contribute to development of lymphedema therapy.

  Corresponding, Aug. 2018, Lymphatic research and biology, 16(4) (4), 390 - 396, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Treatment modalities and risk factors associated with refractory neurosensory disturbances of the inferior alveolar nerve following oral surgery: a multicentre retrospective study

  T. Hasegawa, S. I. Yamada, N. Ueda, S. Soutome, M. Funahara, M. Akashi, S. Furuno, H. Miyamoto, S. Hayashida, R. Amano, K. Mori, Y. Kojima, H. Kurita, T. Kirita, M. Umeda, Y. Shibuya, S. Fujita, T. Komori, Japanese Study Group of Cooperative Dentistry with Medicine (JCDM)

  Little research has been conducted into hypoesthesia, and no studies have elucidated the risk factors for refractory hypoesthesia and compared treatment modalities. The purpose of this multicentre retrospective cohort study was to investigate the relationships between various risk factors, treatment modalities, and refractory hypoesthesia. Risk factors for refractory hypoesthesia after oral surgery were evaluated using univariate and multivariate analysis. To minimize the selection bias associated with a retrospective data analysis, a propensity score analysis was performed between the medication and non-medication groups (65 sites in each group). Moderate or severe hypoesthesia (odds ratio 13.42) and no or late administration of ATP/vitamin B12 (odds ratio 2.28) were significantly associated with refractory hypoesthesia. In the propensity score analysis, the incidence rate of refractory hypoesthesia in the medication group was lower than that in the non-medication group (P < 0.001). This study demonstrated the multivariate relationships between various risk factors, treatment modalities, and refractory hypoesthesia. Moderate or severe hypoesthesia and no or late administration of ATP/vitamin B12 were significantly associated with refractory hypoesthesia. Therefore, clinicians should consider these risk factors and initiate early oral administration of ATP/vitamin B12 in cases of hypoesthesia.

  Churchill Livingstone, Jun. 2018, International Journal of Oral and Maxillofacial Surgery, 47(6) (6), 794 - 801, English

  [Refereed]

  Scientific journal


• 血管柄付遊離皮弁を用いた口腔癌の術後感染に関わる因子の検討

  梶真人, Furudoi Shungo, 岸本恵美, 岩田英治, 高橋淳子, 楠元順哉, Akashi Masaya, Komori Takahide

  ガイドライン導入前の当院の抗菌薬予防投与指針に準じて抗菌薬予防投与を行った口腔癌即時再建手術の術後感染発症例を後ろ向きに調査し、術後感染の発症に影響を与える因子について検討した。当科で気管切開術、口腔癌切除術、頸部郭清術および血管柄付遊離皮弁による即時再建術を施行した40例を対象とした。術後感染を認めた症例は40例中12例で、発症率は30.0%であった。基礎疾患の有無による術後感染発症率に有意差は認められず、アルブミン値およびヘモグロビン値との関連もなかった。手術時間での比較では、術後感染を認めなかった症例の平均手術時間が684.1分であったのに対し、術後感染発症例の平均手術時間は785.0分と長く有意差を認めた。また、術中の出血量においても感染を認めなかった症例の平均出血量が671.9mlであったのに対し、術後感染発症例の平均は1050.3mlと多く有意差を認めた。

  (一社)日本口腔感染症学会, Jun. 2018, 日本口腔感染症学会雑誌, 25(1) (1), 14 - 19, Japanese

  [Refereed]


• Elucidating the masticatory function and oral quality of life according to the range of mandibulectomy

  Michinori Maeda, Marina Hirose, Keinoshin Wada, Megumi Kishimoto, Masaya Akashi, Akira Kimoto, Takahide Komori, Yasuyuki Shibuya

  The aim of this study was to clarify the masticatory function and oral health-related quality of life (OHRQoL) for different ranges of mandibulectomy and also continuity resection of the mandible. A total of thirty one patients who underwent mandibulectomy and were classified into AT, body, and marginal resection (MR) groups according to the CAT classification were investigated. The masticatory function was evaluated using a color-changing gum, a gummy jelly, and a modified Sato's questionnaire. The OHRQoL was evaluated using the Oral Health Impact Profile (OHIP), which included seven subscales. There were significant differences between the AT group and body group in the findings from the color-changing gum and the OHIP (P < 0.05). There was also a significant difference between the AT group and body group regarding the degree of physical disability and psychological disability (subscales of the OHIP) (P < 0.05). There were no significant differences between the body and MR group in any of the tests. Consequently, the body group showed a higher masticatory function and OHRQoL than the AT group despite having the same category of mandibulectomy defect however, the masticatory function and OHRQoL in the body and MR groups were found to be closely similar, at least, regarding the chewing performance.

  Elsevier Ltd, May 2018, Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, 30(3) (3), 220 - 224, English

  [Refereed]

  Scientific journal


• Computed Tomographic Evaluation of Posttreatment Soft-Tissue Changes by Using a Lymphedema Scoring System in Patients with Oral Cancer.

  Masaya Akashi, Shun Teraoka, Yasumasa Kakei, Junya Kusumoto, Takumi Hasegawa, Tsutomu Minamikawa, Kazunobu Hashikawa, Takahide Komori

  BACKGROUND: This study aimed to evaluate posttreatment soft-tissue changes in patients with oral cancer with computed tomography (CT). To accomplish that purpose, a scoring system was established, referring to the criteria of lower leg lymphedema (LE). METHODS AND RESULTS: One hundred and six necks in 95 patients who underwent oral oncologic surgery with neck dissection (ND) were analyzed retrospectively using routine follow-up CT images. A two-point scoring system to evaluate soft-tissue changes (so-called "LE score") was established as follows: Necks with a "honeycombing" appearance were assigned 1 point. Necks with "taller than wide" fat lobules were assigned 1 point. Necks with neither appearance were assigned 0 points. Comparisons between patients with LE score ≥1 and LE score = 0 at 6 months postoperatively were performed using the Fisher exact test for discrete variables and the Mann-Whitney U test for continuous variables. Univariate predictors associated with posttreatment changes (i.e., LE score ≥1 at 6 months postoperatively) were entered into a multivariate logistic regression analysis. Values of p < 0.05 were considered to indicate statistical significance. The occurrence of the posttreatment soft-tissue changes was 32%. Multivariate logistic regression analysis showed that postoperative radiation therapy (RT) and bilateral ND were potential risk factors of posttreatment soft-tissue changes on CT images. CONCLUSIONS: Sequential evaluation of "honeycombing" and the "taller than wide" appearances on routine follow-up CT revealed the persistence of posttreatment soft-tissue changes in patients who underwent oral cancer treatment, and those potential risk factors were postoperative RT and bilateral ND.

  Apr. 2018, Lymphatic research and biology, 16(2) (2), 147 - 153, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 下顎歯肉癌を生じたCowden症候群の1例

  榎本由依, AKASHI MASAYA, 平岡佑二郎, SHIGEOKA MANABU, MINAMIKAWA TSUTOMU, KOMORI TAKAHIDE

  症例は65歳男性で、神経線維腫症の診断で顎口腔領域に放射線療法が施行された。口腔内全体に広がる小腫瘤の精査のため生検を施行し、神経線維腫の診断となった。右側下顎の腫脹・疼痛を認め、パノラマX線写真で放射線性顎骨壊死を疑った。右側下顎臼歯部の近辺に乳頭腫様腫瘤を伴う、易出血性の30mm程度の潰瘍を認め、潰瘍周囲には硬結を触れた。頬粘膜・舌・口蓋粘膜全域に及ぶ多発性の乳頭腫様腫瘤を認めた。パノラマX線写真、CT画像では、右側下顎臼歯部から下顎枝にかけての舌側皮質骨から骨髄質に至る不規則な骨吸収像を認めた。臨床経過や画像所見から右側下顎骨髄炎を疑ったが、悪性腫瘍の可能性もあった。口腔内潰瘍部と潰瘍周囲の腫瘤から生検を施行し、扁平上皮癌と診断した。Cowden症候群の可能性が示唆されたため、下部消化管内視鏡検査とFDG・PETを施行し、下顎歯肉癌を合併したCowden症候群と診断した。下顎歯肉癌に対して全身麻酔下に、気管切開・右側頸部郭清術・下顎半側切除術・右側遊離腹直筋皮弁による即時再建術を施行した。局所および頸部再発等なく経過良好である。

  (公社)日本口腔外科学会, Apr. 2018, 日本口腔外科学会誌, 64(4) (4), 213 - 218, Japanese

  [Refereed]


• Differences between osteoradionecrosis and medication-related osteonecrosis of the jaw.

  Masaya Akashi, Satoshi Wanifuchi, Eiji Iwata, Daisuke Takeda, Junya Kusumoto, Shungo Furudoi, Takahide Komori

  PURPOSE: The appearance of osteoradionecrosis (ORN) and medication-related osteonecrosis of the jaw (MRONJ) is similar, but clinically important differences between ORN and MRONJ exist. The aim of this study was to compare the clinical data between ORN and MRONJ and to reveal the critical differences between these diseases. METHODS: We retrospectively reviewed the epidemiological data, clinical findings, and treatment in 27 ORN and 61 MRONJ patients. Radiographic signs before the initiation of treatment were also assessed. RESULTS: The median age (P = 0.0474) and the ratio of female to male patients (P < 0.0001) were significantly higher in MRONJ patients. There were significantly more MRONJ patients who reported a history of pain when compared with ORN patients (P = 0.0263). As an aetiological factor, tooth extraction was significantly more relevant to MRONJ than ORN (P = 0.0352). When assessing the radiographic signs on computed tomographic images, periosteal reaction was found only in MRONJ patients (P = 0.0158). Minimal debridement was performed significantly more frequently for MRONJ (P = 0.0093), and by contrast, surgical resection was performed more frequently for ORN (P = 0.0002). CONCLUSIONS: Understanding the clinical and underlying pathological differences between ORN and MRONJ probably contributes to the selection of appropriate treatment for each patient.

  Mar. 2018, Oral and maxillofacial surgery, 22(1) (1), 59 - 63, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• スニチニブ投与患者に発症した顎骨壊死の1例

  岩田英治, Furudoi Shungo, 鰐渕聡, 岸本恵実, Akashi Masaya, Komori Takahide

  症例は72歳男性で、左腎癌に対して左腎摘出術を施行した。経過観察していたが、胸部CT画像にて肺癌を指摘された。左肺癌の診断で胸腔鏡補助下左下葉切除術を施行し、病理組織学的に腎細胞癌の肺転移と診断した。経過観察していたが、胸部CT画像で右胸膜・縦隔リンパ節転移を認めた。スニチニブの経口投与を開始したが、6日後に肝機能障害を認めたため中止した。スニチニブを減量し、2週内服1週休薬で施行し、4コース目までが終了した。右下顎大臼歯部の骨露出および同部への舌の接触痛を自覚した。薬剤関連性顎骨壊死と診断し、口腔衛生管理および局所の洗浄を行うこととした。露出骨に対し鋭縁研磨を行い、右舌縁部の潰瘍に対しデキサメタゾン軟膏塗布を行った。スニチニブは減量し、2週内服1週休薬で継続した。2ヵ月後には骨露出部に歯肉の増生を認め、露出範囲は縮小し、右舌縁部の潰瘍は消失した。初診より8ヵ月目の胸部CT画像から肺転移巣にPDが確認され、スニチニブを中止し、新たにアキシチニブの内服を開始した。胸部CT画像から胸膜転移巣の増大ありと判断した。アキシチニブを中止し、新たにオプジーボの内服を開始し、現在も継続中である。

  (公社)日本口腔外科学会, Feb. 2018, 日本口腔外科学会雑誌, 64(2) (2), 83 - 87, Japanese

  [Refereed]


• Four-Dimensional Computed Tomography Evaluation of Condylar Movement in a Patient With Temporomandibular Joint Osteoarthritis.

  Masaya Akashi, Takumi Hasegawa, Satoru Takahashi, Takahide Komori

  Temporomandibular joint (TMJ) osteoarthritis (OA) is a disease of the bone, cartilage, and supporting tissues of the joint. Patients with advanced TMJ-OA often have symptoms such as pain, swelling, and joint dysfunction, and they sometimes require surgical intervention when conservative treatment is not effective. The etiology of TMJ-OA remains elusive. The usefulness of 4-dimensional (4D) computed tomography (CT) in motion analysis of various joints has recently been reported. This article introduces a novel imaging technique of 4D CT that aims to identify kinematic features that may be associated with the etiology of TMJ-OA. In a 69-year-old female patient with severe TMJ-OA, 4D CT evaluation of condylar movement was performed. During the scan, she was instructed to masticate a cookie normally and her natural condylar movement during mastication was evaluated. The coronal 4D CT motion images showed that the synovial cavity was narrower on the affected side than on the unaffected side. Repeated friction between the articular surface of the condyle and the caudal surface of the articular eminence was observed during natural mastication. Although friction between the condyle and articular eminence has been considered a factor in the initiation and progression of TMJ-OA in previous experimental studies using animals, this is the first study to directly visualize the friction between the atrophic and flattened condylar surface and the articular eminence. Four-dimensional CT is a novel imaging technique with the potential to assess kinematic features that cannot be visualized with other imaging modalities in patients with TMJ disease.

  W.B. Saunders, Feb. 2018, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 76(2) (2), 304 - 313, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Facial artery musculomucosal flap to close an oroantral fistula

  Yumi Ohtsuki, Masaya Akashi, Kazunobu Hashikawa, Takahide Komori

  Oroantral fistulas (OAF), which develop as a result of maxillary sinus infection caused by oroantral communication, are sometimes intractable. We present a case of closure with a facial artery musculomucosal flap (FAMM flap) for intractable OAF. A 73-year-old man presenting with an OAF and rhinorrhea was referred to our department. Twenty-eight years earlier, he had undergone bilateral radical surgery for maxillary sinusitis, and six years earlier, he underwent extraction of his right maxillary first and second molars. Six months after the dental extractions, he developed maxillary sinusitis secondary to oroantral communication. Four years after the dental extractions, he underwent endoscopic sinus surgery with closure of the OAF with a rotational pedicled palatal flap, but the OAF recurred. To treat the OAF recurrence, we used a FAMM flap. His postoperative course was good, with no OAF recurrence 6 years after surgery. FAMM flaps are useful to close intractable OAFs.

  Corresponding, Elsevier Ltd, 2018, Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Potential role of post-treatment follow-up FDG-PET CT to detect mandibular osteoradionecrosis: A case report.

  Masaya Akashi, Satoshi Wanifuchi, Junya Kusumoto, Megumi Kishimoto, Yasumasa Kakei, Kazunobu Hashikawa, Takahide Komori

  It was hypothesized that fluorodeoxyglucose (FDG) uptake on post-treatment follow-up positron emission tomography with computed tomography (PET CT; using PET CT to monitor and rule out recurrence and metastasis of head and neck carcinoma) would be useful for detecting and understanding the disease state of osteoradionecrosis (ORN) of the jaw. The present study included 14 patients who developed mandibular ORN following radiation therapy (RT) for head and neck cancer and underwent follow-up PET CT several times following RT. Areas exhibiting FDG uptake were retrospectively assessed on post-treatment follow-up PET CT images and were classified into three types: Spot type: Only spot accumulation of FDG; localized type: Accumulation of FDG restricted to within the bone resorption area; extensive type: Accumulation of FDG extending into surrounding soft tissue. PET classification at the time of clinical diagnosis of mandibular ORN in the 14 patients demonstrated the extensive type in 43%, localized type in 36% and spot type in 21%. An increased area of FDG uptake around the ORN was revealed retrospectively on post-treatment follow-up FDG PET-CT images in 50% of patients. Alterations in PET classification included spot type to localized type in 36% and localized type to extensive type in 14%. A significantly increased number of patients with extensive-type ORN (P=0.026) required surgery. Post-treatment follow-up FDG-PET CT may be useful for early detection and better understanding of ORN.

  Jan. 2018, Molecular and clinical oncology, 8(1) (1), 61 - 67, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A Case Series of Fibro-Osseous Lesions of the Jaws.

  Masaya Akashi, Kenji Matsuo, Manabu Shigeoka, Yasumasa Kakei, Takumi Hasegawa, Akira Tachibana, Shungo Furudoi, Takahide Komori

  The aim of this case series was to reveal the difficulties in diagnosing fibro-osseous lesions with radiological and histopathological examinations and quantify the potential risk of infection to fibro-osseous legions. To analyze the concordance between radiological and histopathological diagnoses, this retrospective case series included patients who were clinically diagnosed with fibro-osseous lesions via radiological findings and excluded the patients who did not undergo histopathological examinations. This study also included the patients in whom histopathological results confirmed fibro-osseous legions when preoperative radiological diagnosis did not include fibro-osseous legions. Eleven patients (three men, eight women; median age 24.5 years, range 15-57 years) were enrolled. Although radiological diagnoses of fibrous dysplasia (FD) corresponded with histopathological diagnoses in seven patients, mismatches between radiological findings and histopathological results were found in three patients. In one patient, suspected diagnosis with radiological examinations was malignant lymphoma or FD. In two patients, the histopathological differentiation between FD and ossifying fibroma (OF) was difficult. One patient had lesion recurrence which was suspected to be OF with surgical findings and postoperative course after the initial surgery. In three patients, infections of FD were found. Preoperative diagnosis of OF with radiographic feature of unilocular radiolucency is difficult. In cases in which histopathological differentiation between FD and OF is difficult, operative findings should be used because OF is often found to be well-encapsulated and easily enucleated. Bone in FD showing mixed radiolucent-radiopaque may be vulnerable to infection.

  Dec. 2017, The Kobe journal of medical sciences, 63(3) (3), E73-E79 - 79, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Dental injuries caused by endotracheal intubation – A retrospective study

  Yasumasa Kakei, Masaya Akashi, Masahiko Kashin, Sayaka Komori, Takahide Komori

  Objective Dental injury is the most common complication of general anaesthesia. The primary purpose of this study was to report the occurrence, location, type, and treatment of dental injury caused by endotracheal intubation. Methods This retrospective study evaluated 36,627 cases of general anaesthesia at our hospital from January 2008 through December 2015. The examination items included risk factors such as age sex surgical department type of intubation(orotracheal or nasotracheal intubation) presence of preoperative trismus Cormack–Lehane classification experience of the attending anaesthesiologist intubation appliance mouth guard use tooth mobility diagnosed by attending anaesthesiologist before surgery the location, type, and treatment of injured teeth and forensic implications. Results The incidence of dental injury was 0.06% (22 of 36,627 patients). There was a significant difference in the rate of dental injury according to age (0.026% among those < 60 years of age vs. 0.096% among those ≥60 years P < 0.05) and according to surgical department (0.189% among neurosurgery patients P < 0.05). The most common Cormack–Lehane classification of laryngeal view in the 22 patients with dental injury was grade 2 (50%). Tooth mobility was diagnosed by anaesthesiologists in 50% of injured patients before surgery. There was no preoperative dental consultation in our department in any of the patients with injured teeth. Conclusions Dental screening is recommended for patients in whom anaesthesiologists find mobile teeth and difficult intubation. A system to facilitate cooperation between anaesthesiologists and dental surgeons should be established.

  Elsevier Ltd, Nov. 2017, Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, 29(6) (6), 518 - 521, English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Axial four-dimensional computed tomographic images to analyze crosswise differences in protrusive condylar movement in patients who underwent mandibulectomy and free flap reconstruction.

  Masaya Akashi, Toshinori Sekitani, Yumi Ohtsuki, Yasumasa Kakei, Junya Kusumoto, Takumi Hasegawa, Michinori Maeda, Noriyuki Negi, Kazunobu Hashikawa, Yasuyuki Shibuya, Satoru Takahashi, Takahide Komori

  OBJECTIVE: Few studies evaluate condylar movement following mandibular reconstruction. The main objective of this study was to show that axial four-dimensional computed tomography (4DCT) could visualize bilateral protrusive condylar movement directly. We used axial 4DCT images to assess condylar protrusion in patients who underwent mandibular reconstruction. METHODS: We enrolled seven healthy volunteers (median age 30 years, range 27-38 years) and seven patients (median age 65 years, range 52-80 years), who underwent mandibulectomy (segmental in five, hemi in one, marginal in one) and free flap reconstruction (using the fibula in six and the radial forearm in one). Six study subjects were instructed to masticate a cookie during the 4DCT scan (the seventh made chewing motions). The distance between the most anterior and posterior positions of the bilateral condyles on 4DCT (axial view) images was then measured and compared between controls and patients using the Mann-Whitney U-test. RESULTS: The crosswise difference in the distances of condylar protrusion was significantly greater in patients than in the controls. CONCLUSION: Axial 4DCT images can visualize a bilateral condylar protrusive path. Axial 4DCT images for patients who have undergone mandibulectomy and reconstruction may be useful for evaluation of functional movement of condyles.

  CHURCHILL LIVINGSTONE, Nov. 2017, Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery, 45(11) (11), 1778 - 1783, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Literature Review of Criteria for Defining Recipient-Site Infection after Oral Oncologic Surgery with Simultaneous Reconstruction.

  Masaya Akashi, Junya Kusumoto, Akiko Sakakibara, Kazunobu Hashikawa, Shungo Furudoi, Takahide Komori

  BACKGROUND: The lack of uniformity of criteria for defining recipient-site infection after oral oncologic surgery with simultaneous reconstruction is problematic despite numerous studies on this issue. This study aimed to investigate the difference in the criteria for defining recipient-site infection after oral oncologic surgery with reconstruction. METHODS: A Medline search was performed via PUBMED using the following combinations of key terms that were tagged in the title, abstract, or both: "surgical site infection-head neck," "surgical site infection-oral cancer," "antibiotic prophylaxis-head neck," and "surgical site infection-oral carcinoma." Search results were filtered between 2005 and 2017. Articles in which there was no mention of the criteria for definition of surgical-site infection were excluded. RESULTS: The number of articles that met the inclusion criteria was 24. The lack of uniformity in the criteria for defining recipient-site infection in each article appeared to be attributable mainly to differences in whether an orocutaneous fistula and superficial incisional infection were regarded as recipient-site infection. CONCLUSION: Reconsideration of the categorization of orocutaneous fistula as infection, regardless of the etiology, and differentiation of superficial and deep incisional infections are necessary for correct assessment of recipient-site infection in oral oncologic surgery.

  MARY ANN LIEBERT, INC, Oct. 2017, Surgical infections, 18(7) (7), 755 - 764, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Targeted Disruption of JCAD (Junctional Protein Associated With Coronary Artery Disease)/KIAA1462, a Coronary Artery Disease-Associated Gene Product, Inhibits Angiogenic Processes In Vitro and In Vivo.

  Tetsuya Hara, Tomoko Monguchi, Noriko Iwamoto, Masaya Akashi, Kenta Mori, Toshihiko Oshita, Mitsumasa Okano, Ryuji Toh, Yasuhiro Irino, Masakazu Shinohara, Yui Yamashita, Go Shioi, Mikio Furuse, Tatsuro Ishida, Ken-Ichi Hirata

  OBJECTIVE: Recent genome-wide association studies newly identified the human KIAA1462 gene as a new locus for coronary artery disease. However, the function of the gene product, named JCAD (junctional protein associated with coronary artery disease), is unknown. Because JCAD is expressed at cell-cell junctions in endothelial cells, we hypothesized and tested whether JCAD regulates angiogenic processes in vitro and in vivo. APPROACH AND RESULTS: Cell culture experiments revealed impaired angiogenic ability (proliferation, migration, and cord formation) by the knockdown of JCAD with siRNA (P<0.05 versus control siRNA). We have generated mice lacking JCAD (mKIAA1462-/-) by gene-targeted deletion of JCAD to address in vivo angiogenic function. mKIAA1462-/- mice did not show morphological differences in development of retinal vasculature. Ex vivo aortic ring model demonstrated impaired neovascularization in aorta from mKIAA1462-/- mice than control wild-type mice (P<0.05). Tumor growth was assessed by monitoring tumor volume after the subcutaneous injection of melanoma, LLC (Lewis lung carcinoma), and E0771 cells into the mice. mKIAA1462-/- mice exhibited significantly smaller tumor volume compared with wild-type mice (P<0.001). Histological assessment of the tumor exhibited less smooth muscle actin-positive neovascularization determined by CD31-positive vascular structure in tumor of mKIAA1462-/- mice than wild-type mice, indicating that knockdown of JCAD inhibited the vascular maturation in pathological angiogenic process. CONCLUSIONS: These in vitro and in vivo studies suggest that JCAD has a redundant functional role in physiological angiogenesis but serves a pivotal role in pathological angiogenic process after birth.

  LIPPINCOTT WILLIAMS & WILKINS, Sep. 2017, Arteriosclerosis, thrombosis, and vascular biology, 37(9) (9), 1667 - 1673, English, International magazine

  [Refereed]

  Scientific journal


• インプラント周囲に放射線性顎骨壊死を生じ保存的に治療した1例

  明石昌也, 鰐渕聡, 岩田英治, 筧康正, Hasegawa Takumi, 鈴木泰明, Komori Takahide

  頭頸部癌に対する放射線治療は重要な治療法であるが、重篤な合併症の一つに放射線性顎骨壊死がある。最も良く知られた発症の誘因は、抜歯などの外科的侵襲である。今回、われわれはインプラント周囲に放射線性顎骨壊死を生じ、長期に渡る保存的治療ののちに腐骨分離した1例を経験したので報告する。症例は68歳の女性。左側顎下腺腺様嚢胞癌の診断のもと、手術と術後化学放射線療法が施行された。放射線治療から3年後、右側下顎に放射線治療前より埋入されていたインプラント周囲溝からの排膿を伴う感染所見を認め、当科を受診した。インプラント周囲の放射線性顎骨壊死の診断のもと、局所の洗浄や抗菌薬経口投与などの保存的治療を継続し、放射線治療から5年後インプラントと腐骨が一塊に分離したため、局所麻酔下に除去した。放射線性顎骨壊死は多くの場合難治性であり、治療法は随伴症状の重篤度や全身状態などを総合的に判断し選択する必要がある。予防にはインプラント周囲の衛生状態の維持を目的とした放射線治療後の口腔衛生管理がきわめて重要である。(著者抄録)

  (公社)日本顎顔面インプラント学会, Aug. 2017, Japanese Journal of Maxillo Facial Implants, 16(2) (2), 75 - 80, Japanese

  [Refereed]


• Changes in cell junctions induced by inhibition of epidermal growth factor receptor in oral squamous cell carcinoma cells.

  Yasumasa Kakei, Shun Teraoka, Masaya Akashi, Takumi Hasegawa, Takahide Komori

  The benefits of epidermal growth factor receptor (EGFR) targeting in the treatment of head and neck cancer, have been documented. However, a minority of patients with head and neck cancer are unresponsive to EGFR targeting therapies. The present study evaluated the effects and limitations of an EGFR inhibitor on oral squamous cell carcinoma cells, particularly on cell-cell junctions mediated by epithelial (E)-cadherin. HSC-3 oral squamous cell carcinoma cells were treated with the EGFR inhibitor, AG1478 (0, 0.5, 2, 10 and 50 µM), and the effects of EGFR inhibition in HSC-3 cells were evaluated by wound healing assays, E-cadherin immunostaining and measurement of transepithelial electrical resistance in vitro. It was observed that treatment of oral squamous cell carcinoma cells with AG1478 suppressed cell motility, altered cell morphology and increased the number of cell-cell junctions compared with untreated control cells. Knockdown of EGFR induced a similar phenotype to that observed by the inhibition of EGFR. Furthermore, in oral squamous cell carcinoma cells treated with high-dose EGFR inhibitor (50 µM), the small number of cells that survived formed cell-cell junctions that were positive for E-cadherin expression. In cells treated with low concentrations of EGFR inhibitor (2 µM), recovery of epithelial properties was observed. The retention of E-cadherin expression in cells that survived high-dose EGFR inhibitor treatment may be a survival mechanism of cancer cells.

  Corresponding, SPANDIDOS PUBL LTD, Aug. 2017, Experimental and therapeutic medicine, 14(2) (2), 953 - 960, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 頭頸部癌患者における化学放射線療法による口腔粘膜炎の重症度を予測する因子

  岸本恵実, Furudoi Shungo, 岩田英治, 藤林淳子, 楠元順哉, 明石昌也, Komori Takahide

  術後追加療法として化学放射線療法(CRT)を行った口腔癌21例、中咽頭癌26例の計47例(男33例、女14例、平均年齢59.9歳、範囲16〜76歳)を対象に、頭頸部癌に対するCRT中の口腔粘膜炎の重症度を予測する因子について、後ろ向きに検討した。CRT中に出現した口腔粘膜炎のGrade別はGrade 2が40例(85.1%)、Grade 3が7例(14.9%)であり、背景因子の比較では栄養学的予後指数(PNI)に有意な群間差を認めた。また、Grade 3出現例ではGrade 2出現例よりも末梢血総リンパ球数、血清アルブミン濃度が有意に低値を示し、算出したCRT開始前のPNIについてROC曲線のカットオフ値は41.1で、AUC:0.86、感度:0.95、特異度:0.71であった。PNIは口腔粘膜炎の重症度を予測する因子となる可能性が示唆された。

  (一社)日本口腔感染症学会, Jul. 2017, 日本口腔感染症学会雑誌, 24(1) (1), 9 - 13, Japanese

  [Refereed]


• Postoperative drainage in head and neck surgery for oral cancer

  Izumi Saito, Takumi Hasegawa, Eiji Iwata, Natsuki Yonezawa, Satomi Arimoto, Daisuke Takeda, Akiko Sakakibara, Masaya Akashi, Tsutomu Minamikawa, Takahide Komori

  Objective Previous reports regarding postoperative drainage have documented complications associated with the drainage volume (DV). However, there is little information available concerning the postoperative and operative factors that influence postoperative DV. In this study, we retrospectively investigated the relationship between postoperative drainage and various risk factors in patients undergoing head and neck surgery for oral cancer. Methods This study included 136 patients (88 males, 48 females, mean age 67.2 ± 12.9 years) who underwent head and neck surgery for oral cancer. We retrospectively investigated the following variables: age, sex, subsite, medical history, surgical procedure, operative time, blood loss, duration of drain retention, DV, and postoperative complications. Results DV after reconstruction with a pectoral major musculocutaneous flap was significantly higher than DV after reconstruction with a forearm flap (p <  0.05). DV was significantly greater in cases involving a large amount of blood loss or a long operative time (p <  0.05). DV was significantly greater after modified radical neck dissection than after supraomohyoid or submandibular neck dissection (p <  0.05). DV was significantly greater in patients receiving antithrombotic therapy than in patients without (p <  0.05). Multiple regression analysis revealed that total DV was positively correlated with older age, antithrombotic therapy, type of surgery (advanced surgery), and type of neck dissection (wide range). Conclusions DV was greater in those with older age, antithrombotic therapy, advanced surgery, and wide-ranging neck dissection. Therefore, clinicians should consider these risk factors and pay special attention to the postoperative management of drains in such cases.

  Elsevier Ltd, May 2017, Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, 29(3) (3), 217 - 221, English

  [Refereed]

  Scientific journal


• 肺癌術後肺合併症と周術期口腔機能管理の有用性

  岩田 英治, 長谷川 巧実, 西井 美佳, 明石 昌也, 古森 孝英

  (一社)日本口腔ケア学会, Apr. 2017, 日本口腔ケア学会雑誌, 11(3) (3), 170 - 170, Japanese

  [Refereed]


• Brain Abscess Potentially Resulting from Odontogenic Focus: Report of Three Cases and a Literature Review.

  Masaya Akashi, Kazuhiro Tanaka, Junya Kusumoto, Shungo Furudoi, Kohkichi Hosoda, Takahide Komori

  INTRODUCTION: Odontogenic foci can rarely cause intracranial infection. Hematogenous spread is considered to be the most important pathophysiological mechanism of intracranial infection of odontogenic origin. To investigate the oral origin of intracranial infections, oral surgeons should understand the underlying mechanisms by which oral bacteria spread to the central nervous system. However, there have been very few reports of intracranial infection resulting from odontogenic infection. CASE REPORTS: The authors report the cases of a 64-year-old man, a 68-year-old man, and a 64-year-old woman whose brain abscesses perhaps have arisen from odontogenic foci, because other sources of intracranial infection such as endocarditis and maxillary sinusitis were not found. Bacteriological examination of brain abscess specimens identified Staphylococcus aureus in case 1, Streptococcus constellatus, Fusobacterium nucleatum, and Parvimonas micra in case 2, and Lactobacillus catenaformis, Porphyromonas gingivalis, and F. nucleatum in case 3. All suspected causal teeth had no obvious signs of acute inflammation in all three cases. CONCLUSIONS: Oral surgeons should understand these characteristics of odontogenic brain abscess, in which the potentially causal odontogenic foci often lack acute symptoms. If other origins of infection are not found, it would be better to eliminate the potentially causal odontogenic foci for improvement of oral hygiene, however, the decision making criteria to eliminate suspected causal teeth is needed to be elucidated.

  Mar. 2017, Journal of maxillofacial and oral surgery, 16(1) (1), 58 - 64, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Meaning and Limitation of Cortical Bone Width Measurement with DentaScan in Medication-Related Osteonecrosis of the Jaws.

  Eiji Iwata, Masaya Akashi, Megumi Kishimoto, Junya Kusumoto, Takumi Hasegawa, Shungo Furudoi, Takahide Komori

  Mandibular cortical bone measurement with x-ray imaging is known to be a potentially useful tool in the detection of dimensional changes caused by bisphosphonate. The primary purpose of this study was to assess the meaning and limitation of cortical bone measurement with computed tomography (CT) in patients with medication-related osteonecrosis of the jaw (MRONJ). The investigators obtained DentaScan images of the mandible from 15 patients with MRONJ, 15 patients with a history of antiresorptive agent administration without symptoms of MRONJ (non-MRONJ), and 15 control subjects. The cortical bone width measured on DentaScan images was compared between the three groups (ANOVA and Tukey's test). Interobserver reliability between two observers was also assessed. The values of interclass correlation coefficient were 0.48 in the MRONJ group, 0.29 in the Non-MRONJ group, and 0.34 in control group. The cortical bone widths calculated both by observer 1 and observer 2 were thicker in patients with MRONJ than in the non-MRONJ group and controls. There were significant differences in cortical bone width among the MRONJ, non-MRONJ, and control groups in observer 1 (P < 0.001) and observer 2 (P < 0.001), specifically comparing the MRONJ group with the non-MRONJ group and the control group. Cortical bone width measurement is useful for the distinction between medication-related osteonecrosis of the jaw and normal bone, in spite of the low interobserver reliability.

  Corresponding, Kobe University School of Medicine, Feb. 2017, The Kobe journal of medical sciences, 62(5) (5), E114-E119 - E119, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Survival of Brånemark System Mk III implants and analysis of risk factors associated with implant failure

  T. Hasegawa, S. Kawabata, D. Takeda, E. Iwata, I. Saito, S. Arimoto, A. Kimoto, M. Akashi, H. Suzuki, T. Komori

  The purpose of this study was to retrospectively investigate the outcomes of Brånemark System Mk III TiUnite/Groovy implants placed in patients at Kobe University Hospital. Various risk factors for implant failure, including mechanical coupling, were investigated by univariate and multivariate analysis. The predictive variables investigated included age, sex, smoking habit, general health, history of radiation therapy, application of a dentomaxillary prosthesis, type of prosthesis, use of alveolar bone augmentation, site of implant insertion, mechanical coupling between implants, and the length and diameter of the implants. Of the 907 implants investigated, only 23 were unsuccessful the overall survival rate was 96.7%. Increased age, radiation therapy, application of a removable prosthesis or dentomaxillary prosthesis, lack of mechanical coupling between implants, and shorter implants (≤8.5 mm) were significant risk factors for implant failure according to univariate analysis (P <  0.05). Multivariate analysis identified a significant association (P <  0.05) between dental implant failure and a lack of mechanical coupling between implants (odds ratio 6.88) and shorter implants (≤8.5 mm) (odds ratio 3.43). The findings of this study demonstrated multivariate relationships between various risk factors and dental implant failure.

  Churchill Livingstone, Feb. 2017, International Journal of Oral and Maxillofacial Surgery, 46(2) (2), 267 - 273, English

  [Refereed]

  Scientific journal


• Upregulated Expression of Transient Receptor Potential Cation Channel Subfamily V Receptors in Mucosae of Patients with Oral Squamous Cell Carcinoma and Patients with a History of Alcohol Consumption or Smoking.

  Akiko Sakakibara, Shunsuke Sakakibara, Junya Kusumoto, Daisuke Takeda, Takumi Hasegawa, Masaya Akashi, Tsutomu Minamikawa, Kazunobu Hashikawa, Hiroto Terashi, Takahide Komori

  OBJECTIVES: Transient receptor potential cation channel (subfamily V, members 1-4) (TRPV1-4) are expressed in skin and neurons and activated by external stimuli in normal mucosae of all oral cavity sites. The oral cavity is exposed to various stimuli, including temperature, mechanical stimuli, chemical substances, and changes in pH, and, notably, the risk factors for oncogenic transformation in oral squamous epithelium are the same as the external stimuli received by TRPV1-4 receptors. Hence, we examined the relationship between oral squamous cell carcinoma (SCC) and TRPV1-4 expression. MATERIALS AND METHODS: Oral SCC patients (n = 37) who underwent surgical resection were included in this study. We investigated the expression of TRPV1-4 by immunohistochemical staining and quantification of TRPV1-4 mRNA in human oral mucosa. In addition, we compared the TRPV1-4 levels in mucosa from patients with SCC to those in normal oral mucosa. RESULTS: The receptors were expressed in oral mucosa at all sites (tongue, buccal mucosa, gingiva, and oral floor) and the expression was stronger in epithelia from patients with SCC than in normal epithelia. Furthermore, alcohol consumption and tobacco use were strongly associated with the occurrence of oral cancer and were found to have a remarkable influence on TRPV1-4 receptor expression in normal oral mucosa. In particular, patients with a history of alcohol consumption demonstrated significantly higher expression levels. CONCLUSION: Various external stimuli may influence the behavior of cancer cells. Overexpression of TRPV1-4 is likely to be a factor in enhanced sensitivity to external stimuli. These findings could contribute to the establishment of novel strategies for cancer therapy or prevention.

  PUBLIC LIBRARY SCIENCE, 2017, PloS one, 12(1) (1), e0169723, English, International magazine

  [Refereed]

  Scientific journal


• Prognosis of oral squamous cell carcinoma patients with level IV/V metastasis: An observational study.

  Takumi Hasegawa, Yasuyuki Shibuya, Daisuke Takeda, Eiji Iwata, Izumi Saito, Yasumasa Kakei, Akiko Sakakibara, Masaya Akashi, Tsutomu Minamikawa, Takahide Komori

  PURPOSE: The objectives of this study were to retrospectively describe the characteristics and outcomes of patients with oral squamous cell carcinoma (OSCC) with level IV/V metastases, and to evaluate the multivariate relationships among potential risk factors for metastasis and prognosis. MATERIALS AND METHODS: We evaluated 291 patients (178 men and 113 women; mean age, 65.9 ± 13.5 years). Clinicopathological data, time of development of level IV/V metastases, and clinical course were investigated. RESULTS: Twenty-three patients (7.9%) developed level IV/V metastases. The 3-year overall survival rates when level IV/V metastasis first developed were 27.3% upon initial treatment, 57.1% when metachronous neck metastasis developed, and 40.0% when the tumor recurred. Oral tongue tumor subsite, high N staging, neck dissection when metachronous neck metastasis developed, as well as recurrence were independent risk factors for level IV/V metastasis. CONCLUSION: We demonstrate here the multivariate relationships among the risk factors indicated above for level IV/V metastasis and their prognostic significance for patients with OSCC. Oral tongue tumors, high N staging, and neck dissection upon the occurrence of metachronous neck metastasis or recurrence were risk factors for level IV/V metastasis and positive extracapsular spread, presence of multiple lymph metastases, and moderate or poor differentiation were poor prognostic factors.

  CHURCHILL LIVINGSTONE, Jan. 2017, Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery, 45(1) (1), 145 - 149, English, International magazine

  [Refereed]

  Scientific journal


• Intensity and duration of neutropenia relates to the development of oral mucositis but not odontogenic infection during chemotherapy for hematological malignancy.

  Megumi Kishimoto, Masaya Akashi, Kazuyuki Tsuji, Junya Kusumoto, Shungo Furudoi, Yasuyuki Shibuya, Yumiko Inui, Kimikazu Yakushijin, Shinichiro Kawamoto, Atsuo Okamura, Hiroshi Matsuoka, Takahide Komori

  BACKGROUND: D-index which combines the intensity and duration of neutropenia is reported as a tool for evaluating the dynamics of neutropenia. This study aimed to analyze the relationship between D-index and oral complications (i.e., oral mucositis [OM] and odontogenic infection [OI]) during chemotherapies for hematological malignancies. METHODS: A total of 421 chemotherapeutic courses in 104 patients were analyzed. Chemotherapeutic courses in patients who finished all of the prophylactic dental treatments were defined as "treatment Finish". Chemotherapeutic courses in patients who did not finish prophylactic dental treatments were defined as "treatment not-Finish". OM was evaluated according to the Common Terminology Criteria for Adverse Events, version 4.0. D-index was compared between chemotherapeutic courses with versus without oral complications. RESULTS: D-index was significantly higher in chemotherapeutic courses with grade 1 or 2 OM (p < 0.001) than courses without OM. In contrast, higher D-index did not relate to the development of OI (p = 0.18). The occurrence of OI (p < 0.001) but not OM (p = 0.56) during chemotherapy was significantly higher in chemotherapeutic courses without the completion of dental intervention. CONCLUSIONS: Higher D-index relates to the development of OM. In contrast, OI occurs due to untreated odontogenic foci, and its occurrence does not relate to higher D-index.

  Corresponding, PUBLIC LIBRARY SCIENCE, 2017, PloS one, 12(7) (7), e0182021, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Heterogeneity of Necrotic Changes between Cortical and Cancellous Bone in Mandibular Osteoradionecrosis: A Histopathological Analysis of Resection Margin after Segmental Mandibulectomy.

  Masaya Akashi, Kazunobu Hashikawa, Satoshi Wanifuchi, Junya Kusumoto, Manabu Shigeoka, Shungo Furudoi, Hiroto Terashi, Takahide Komori

  BACKGROUND: This study aimed to analyze differences in necrotic changes between cortical and cancellous bone in resection margins after segmental mandibulectomy for advanced mandibular osteoradionecrosis. METHODS: Anteroposterior bone specimens from eleven patients who underwent segmental mandibulectomy with simultaneous free fibula flap reconstruction for advanced osteoradionecrosis were analyzed histopathologically for the presence of necrotic bone based on the presence of blood vessels within Haversian canals. RESULTS: Ten of eleven (91%) cortices near the inferior border of the mandible at the anterior margins were necrotic. All cancellous bones at the anterior margins were viable. Seven of eleven (64%) cortices near the inferior border of the mandible at the posterior margins were necrotic. Three of eleven (27%) cancellous bones at the posterior margins were necrotic. CONCLUSION: Necrotic changes are more prevalent in cortices than in cancellous bones in mandibular osteoradionecrosis, probably due to a decrease of periosteal blood supply caused by radiotherapy.

  HINDAWI LTD, 2017, BioMed research international, 2017, 3125842 - 3125842, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Clinical Analysis of Tooth Injuries in Elderly Person

  古土井 春吾, 可信 雅彦, 筧 康正, 松本 耕祐, 明石 昌也, 古森 孝英

  外傷歯の特徴は年齢層で相違がある。今回われわれは、高齢者の外傷歯の特徴を明らかにする目的で、2008年1月から2015年12月までに神戸大学医学部附属病院歯科口腔外科を受診した外傷歯患者188例(497歯)を対象に調査した。対象症例の年齢を世代別に0〜12歳:43例(93歯)、13〜39歳:81例(215歯)、40〜64歳:38例(108歯)、65歳以上:26例(80歯)の4つのグループに分類した。男女比は全体では1.6:1で男性に多かったが、65歳以上では女性の比率が高かった。受傷原因では、0〜12歳と65歳以上では転倒が高い割合を示した。顎骨骨折は13〜39歳で最も多く認められ、65歳以上の割合は30.8%であった。歯の受傷様式では、全世代で不完全脱臼の頻度が高く、65歳以上では歯根破折の割合が他の世代より高かった。老齢人口は増加し続けることから、高齢者の外傷歯も増加すると予測される。高齢者の顎顔面外傷を予防するための対策の検討が必要である。(著者抄録)

  日本外傷歯学会, Dec. 2016, 日本外傷歯学会雑誌, 12(1) (1), 53 - 59, Japanese


• Cause and occurrence timing of osteoradionecrosis of the jaw: a retrospective study focusing on prophylactic tooth extraction.

  Satoshi Wanifuchi, Masaya Akashi, Yasuo Ejima, Hirotaka Shinomiya, Tsutomu Minamikawa, Shungo Furudoi, Naoki Otsuki, Ryohei Sasaki, Ken-Ichi Nibu, Takahide Komori

  PURPOSE: This retrospective study aimed to analyze the relationship between tooth extraction and osteoradionecrosis (ORN) occurrence. The irradiation field, dose, and time interval between radiotherapy (RT) and ORN were reviewed. We also discuss appropriate guidelines for prophylactic tooth extraction. METHODS: A total of 33 patients treated for grade ≥2 (clinical) ORN in our department from 2002 to 2014 were enrolled. The following epidemiological data were retrospectively gathered: age, sex, histological diagnosis, primary tumor sites, radiation dose, chemotherapy, site of ORN, relationship between tooth extraction and ORN occurrence, and time interval between tooth extraction and the initiation or end of RT. RESULTS: Twenty-one percent of ORN cases resulted from tooth extraction. The most common site of ORN (82 %) was the mandibular molar region. About half of ORN cases (49 %) occurred within 2 years after RT. All patients who received tooth extraction after RT developed ORN (100 %) independently of time interval between tooth extraction and the end of RT (median interval, 37.5 months; range, 27-120 months). In contrast, only 50 % of patients who received tooth extraction before RT developed ORN. There may have been an association between the irradiation field and the site of ORN development CONCLUSIONS: ORN occurrence due to tooth extraction was 21 %. Occurrence timing of ORN did not depend on time interval between tooth extraction and the end of RT. The irradiation field is certainly related to the site of ORN; therefore, prophylactic tooth extraction should be performed in consideration of the proposed radiation field and dose.

  Corresponding, Dec. 2016, Oral and maxillofacial surgery, 20(4) (4), 337 - 342, English, International magazine

  [Refereed]

  Scientific journal


• Postradiotherapy Dental Implant Insertion Into Bone Grafts Harvested From Nonirradiated Tissue: Case Reports.

  Akira Kimoto, Yasuyuki Shibuya, Masaki Kobayashi, Masaya Akashi, Takumi Hasegawa, Hiroaki Suzuki, Takahide Komori

  BACKGROUND: Prostheses and dental implants are often used to aid oral rehabilitation after surgery (with/without radiotherapy) for oral cancer. However, some studies have reported that the insertion of dental implants into irradiated bone results in a higher frequency of implant failure than the insertion of such implants in nonirradiated bone. MATERIALS AND METHODS: This report describes the cases of 4 patients with oral cancer who underwent surgery and radiotherapy (total dose: 50-86 Gy) and then had dental implants inserted within the irradiated area. In each case, an ilium bone graft or a latissimus dorsi myocutaneous flap containing scapular bone was transferred to the dental implant site before the insertion of the implants. RESULTS: Twenty-three implants were inserted. After loading, 2 implants were lost, and 21 remained stable. CONCLUSION: In patients who have undergone radiotherapy for oral cancer, transferring bone grafts harvested from nonirradiated tissue to the irradiated site before implant insertion might help to improve dental implant survival rates.

  LIPPINCOTT WILLIAMS & WILKINS, Oct. 2016, Implant dentistry, 25(5) (5), 715 - 9, English, International magazine

  [Refereed]

  Scientific journal


• Comparison between primary closure and skin grafts of the free fibula osteocutaneous flap donor site.

  Masaya Akashi, Kazunobu Hashikawa, Hiroyuki Takasu, Kazuhiro Watanabe, Junya Kusumoto, Akiko Sakakibara, Takumi Hasegawa, Tsutomu Minamikawa, Takahide Komori

  PURPOSE: This study aimed to compare the size of skin paddles, the postoperative course, and donor site complications between primary closure and skin grafts of the free fibula flap donor site. METHODS: Thirty-five consecutive patients were enrolled. Medical records were retrospectively reviewed for risk factors for delayed healing, size of skin paddles, time to resumption of gait with a mobility aid and self-ambulation, early donor site morbidity, and late donor site complaints. RESULTS: The harvested skin paddles were significantly wider in the skin graft group than in the primary closure group (P = 0.02), with no difference in length (P = 0.1). The difference in time to resuming gait with a mobility aid was also significant (P = 0.01), but not the time to self-ambulation (P = 0.9). Two early donor site morbidities (5.7 %) and 12 late donor site complaints (34.3 %) were found. No significant difference in the incidence of early donor site morbidity was observed between two groups. Occurrence of late donor site complaints was not affected by any risk factors. CONCLUSIONS: The width of the harvested skin paddle, but not the length, is one of factors involved in donor site closure. Resumption of gait with a mobility aid, but not self-ambulation, may be delayed in skin graft patients.

  Springer Verlag, Sep. 2016, Oral and maxillofacial surgery, 20(3) (3), 233 - 7, English, International magazine

  [Refereed]

  Scientific journal


• Four-dimensional computed tomography evaluation of jaw movement following mandibular reconstruction: A pilot study.

  Masaya Akashi, Yasuyuki Shibuya, Satoru Takahashi, Kazunobu Hashikawa, Takumi Hasegawa, Yasumasa Kakei, Noriyuki Negi, Toshinori Sekitani, Takahide Komori

  PURPOSE: The purpose of this study was to analyze jaw movement during mastication in patients who underwent mandibular reconstruction following segmental mandibulectomy, including mouth opening and mastication time, based on four-dimensional computed tomography (4D CT) images. MATERIAL AND METHODS: This study included six surgical patients, who underwent segmental mandibulectomy and simultaneous reconstruction with a free fibula osteocutaneous flap, and four controls. 4D CT was performed during mastication of a cookie to evaluate the movement of the jaw during natural function. The maximum mouth opening, mastication time, and movement of the mandibular angle during mastication were evaluated from the 4D CT images. RESULTS: 4D CT images enabled visualization of jaw movement during mastication. When compared with the controls, the maximum mouth opening during mastication and excursion of the mandibular angle, especially on the diseased side, tended to decrease in surgical patients; however, this did not occur with mastication time. The numerical differences between the diseased and nondiseased side in surgical patients tended to be higher than the crosswise differences in controls. CONCLUSION: 4D CT images revealed differences in jaw function between patients who underwent mandibular reconstruction and controls. 4D CT could be used to evaluate postoperative outcomes following mandibular reconstruction.

  CHURCHILL LIVINGSTONE, May 2016, Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery, 44(5) (5), 637 - 41, English, International magazine

  [Refereed]

  Scientific journal


• 対側オトガイ部の知覚異常が早期症状と疑われたびまん性大細胞型B細胞性リンパ腫の1例

  高端大希, 明石昌也, 高橋佑輔, 辻和志, 南川勉, Komori Takahide

  症例は73歳女性で、右側顎下部から口唇にかけて知覚鈍麻と疼痛を自覚した。初診時、三叉神経痛を疑いカルバマゼピンを開始した。症状の改善を認めないため、下顎神経の神経障害性疼痛を疑いプレガバリンを開始した。就寝前の断続的な激痛は軽快したが、薬剤性アレルギーに起因すると考えられる発疹を生じた。ガバペンチン、クロナゼパムに変更したが、その後も右側の知覚鈍麻は残存した。この頃より右側オトガイ部と類似した知覚鈍麻が左側オトガイ部にも出現した。初診より5ヵ月後、左側頬部から左側臼歯部歯肉に腫脹を認め、画像検査を施行した。顔面CTおよびMRIのSTIR画像で左側下顎骨周囲軟組織の腫脹を認めた。画像検査から数日後には左側頬部の腫脹が急激に増大し、緊急入院となった。左側顎下部より穿刺吸引細胞診を施行し、クロマチン増量を伴うN/C比の高い異型細胞を認め悪性腫瘍を疑った。左側臼歯部歯肉から腫瘤性病変を切開生検し、び漫性大細胞型B細胞性リンパ腫と確定診断した。R-CHOP療法を開始し、腫瘍は縮小したが、両側に及ぶ知覚鈍麻は残存している。

  (公社)日本口腔外科学会, Apr. 2016, 日本口腔外科学会雑誌, 62(4) (4), 163 - 168, Japanese

  [Refereed]


• A comparison of radial forearm free-flap reconstruction and non-reconstruction after partial maxillectomy

  Akashi Masaya, ShibuyaY, TakahashiY, KusumotoJ, SakakibaraA, HasegawaT, MinamikawaT, HashikawaK, KomoriT

  2016, J Oral Maxillofac Surg Med Pathol, 28(2) (2), 111 - 117, English

  [Refereed]

  Scientific journal


• Incidence of Venous Thromboembolism After Oral Oncologic Surgery With Simultaneous Reconstruction.

  Yasumasa Kakei, Masaya Akashi, Takumi Hasegawa, Tsutomu Minamikawa, Satoshi Usami, Takahide Komori

  PURPOSE: Patients with oral cancer who undergo resection with simultaneous reconstruction are presumed to be at high risk for developing venous thromboembolism (VTE) according to current criteria. The primary purpose of this retrospective study was to report the incidence of VTE after oral cancer surgery requiring primary reconstruction and to identify the potential risk factors for VTE in this population. MATERIALS AND METHODS: This retrospective study evaluated 133 consecutive patients who had undergone oral cancer resection with simultaneous reconstruction from April 2007 through December 2014. Bilateral lower-extremity venous duplex ultrasonography (VDUS) was routinely performed 2 days after surgery. Pulmonary embolism was confirmed with computed tomography. The Caprini risk score (CRS) was obtained for each patient. Patients with and without VTE were compared using the χ2 test and Student t test with some variables. Univariate predictors associated with VTE were entered into a multivariate logistic regression analysis. A P value less than .05 was regarded as indicating statistical significance. RESULTS: The incidence of VTE after oral oncologic surgery with simultaneous reconstruction was 26.3% (35 of 133 patients): deep vein thrombosis (DVT) and pulmonary embolism occurred in 2.3% (3 of 133) and DVT alone was found in 24.0% (32 of 133). Multivariate logistic regression analysis showed that only a high CRS was statistically relevant to VTE occurrence. CONCLUSIONS: Because a high incidence of VTE was found after major oral and maxillofacial surgery, lower-extremity VDUS should be undertaken in patients who undergo oral cancer surgery with simultaneous reconstruction, especially those with a high CRS.

  Corresponding, W B SAUNDERS CO-ELSEVIER INC, Jan. 2016, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 74(1) (1), 212 - 7, English, International magazine

  [Refereed]

  Scientific journal


• Temporal Evaluation of Neurosensory Complications After Mandibular Third Molar Extraction: Current Problems for Diagnosis and Treatment.

  Masaya Akashi, Yujiro Hiraoka, Takumi Hasegawa, Takahide Komori

  OBJECTIVE: This retrospective study aimed to report the incidence of neurosensory complications after third molar extraction and also to identify current problems and discuss appropriate management of these complications. METHOD: Patients who underwent extraction of deeply impacted mandibular third molars under general anesthesia were included. The following epidemiological data were retrospectively gathered from medical charts: type of neurosensory complication, treatment for complication, and outcome. RESULTS: A total 369 mandibular third molars were extracted in 210 patients under general anesthesia during this study period. Thirty-one of the 369 teeth (8.4%) in 31 patients had neurosensory complications during the first postoperative week resulting from inferior alveolar nerve damage. Neurosensory complications lasting from 1 to 3 months postoperatively included 17 cases of hypoesthesia and 8 of dysesthesia in 19 patients. Five cases of hypoesthesia and 4 of dysesthesia in 5 patients persisted over 1 year postoperatively. Sixteen of 369 teeth (4.3%) in 16 patients had persistent neurosensory complications after third molar extraction under general anesthesia. Stellate ganglion block was performed in 4 patients. Early initiation of stellate ganglion block (within 2 weeks postoperatively) produced better outcomes than late stellate ganglion block (over 6 months postoperatively). CONCLUSION: Refractory neurosensory complications after third molar extraction often combine both hypoesthesia and dysesthesia. Current problems in diagnosis and treatment included delayed detection of dysesthesia and the lack of uniform timing of stellate ganglion block. In the future, routinely inquiring about dysesthesia and promptly providing affected patients with information about stellate ganglion block might produce better outcomes.

  Bentham Science Publishers B.V., 2016, The open dentistry journal, 10, 728 - 732, English, International magazine

  [Refereed]

  Scientific journal


• ロケーター・磁性バーアタッチメントによるインプラントオーバーデンチャー関連合併症

  有本智美, Hasegawa Takumi, 斉藤泉, 岩田英治, 武田大介, Akashi Masaya, Suzuki Yasuaki, 古土井春吾, Komori Takahide

  (公社)日本顎顔面インプラント学会, Nov. 2015, Japanese Journal of Maxillo Facial Implants, 14(3) (3), 261 - 261, Japanese


• 薬剤関連性顎骨壊死(MRONJ)の1例

  岩田英治, Akashi Masaya, 後藤育子, Furudoi Shungo, Komori Takahide

  症例は68歳男性、大動脈弁狭窄症に対して大動脈弁置換術を施行した。胸腹部CT画像にて右下葉にスリガラス様不透過像と左下葉結節影を指摘され、肺腺癌と診断した。化学療法を施行し、4コース終了後の胸腹部CT画像にて左下葉結節影の増大や胸水の増量などからPDと診断した。追加治療としてシスプラチンとペメトレキセドを併用した化学療法を2週ごとに施行した。骨盤転移に対しては、ゾレドロン酸を1回、デノスマブを毎月1回投与した。食事時に口腔内の接触痛を自覚し、両側下顎臼歯部の骨露出を指摘された。保存的に経過観察していたが、接触痛の増悪を自覚した。両側下顎大臼歯部舌側に10mm大の骨出と、周囲歯肉の発赤、接触痛および軽度腫脹を認めた。薬剤関連下顎骨壊死と診断し、口腔衛生管理および局所の洗浄を行った。デノスマブ投与は中止した。体調不良により通院困難となり、多臓器不全のため初診より6ヵ月後に死亡した。

  (一社)日本口腔診断学会, Oct. 2015, 日本口腔診断学会雑誌, 28(3) (3), 208 - 212, Japanese

  [Refereed]


• 頭頸部領域における壊死性筋膜炎に対するLRINEC scoreの有用性の検討

  楠元順哉, Furudoi Shungo, 榊原晶子, 畑みどり, Akashi Masaya, Komori Takahide

  2001年1月〜2013年12月に経験した18歳以上の壊死性筋膜炎(NF)10例(男性3例、女性7例、39〜81歳、平均65.4歳)、同時期に入院加療した重症蜂窩織炎86例(男性48例、女性38例、18〜90歳、平均56.4歳)を対象に、鑑別診断におけるLRINECスコアの有用性について検討した。NF群は女子が多く、重症蜂窩織炎は男性に多いが、両者に年齢、性別で有意差はなく、血液検査データではCRP、CreでNF群と重症蜂窩織炎間に有意差を認めた。頭頸部NFに対するLRINECスコアの有用性については、カットオフ値を6点とすると、感度90%、特異度88.4%、PPV 47.4%、NPV 98.7%、LR+7.7、LR-0.11で除外診断に有用と思われた。

  (公社)日本口腔外科学会, Oct. 2015, 日本口腔外科学会雑誌, 61(10) (10), 505 - 512, Japanese

  [Refereed]


• CT Evaluation of Morphology of Transferred Fibula for Implant Placement in Reconstructed Mandible

  Masaya Akashi, Yasuyuki Shibuya, Satoshi Wanifuchi, Junya Kusumoto, Akiko Sakakibara, Akira Kimoto, Takumi Hasegawa, Hiroaki Suzuki, Kazunobu Hashikawa, Takahide Komori

  Background:Dental rehabilitation with osseointegrated implants in reconstructed mandibles remains one of the most challenging procedures for oral and maxillofacial surgeons. Satisfactory outcome requires appropriate assessment of graft morphology. There are few analyses of the morphology of fibulae in reconstructed mandibles, although cadaver studies on fibular shape have been performed.Materials and Methods:In this study, we used postoperative computed tomography to retrospectively evaluate the shape, height, and orientation of fibulae transferred after mandibulectomy in 19 patients.Results:The average height of transferred fibulae was 14.3 mm (range, 10.8-20.5 mm). The cross-sectional morphology of transferred fibulae could be classified into 2 types: apex and nonapex. The former type included knife-edged and triangular shapes; the latter included square and circular shapes.Conclusion:When implant insertion is planned in a reconstructed mandible, the orientation of the apex of transferred fibula should be evaluated preoperatively to allow for adjustments in implant procedure because the ridge at the apex of the fibula is narrow.

  LIPPINCOTT WILLIAMS & WILKINS, Oct. 2015, IMPLANT DENTISTRY, 24(5) (5), 541 - 546, English

  [Refereed]

  Scientific journal


• Risk factors associated with postoperative delirium after surgery for oral cancer

  Takumi Hasegawa, Izumi Saito, Daisuke Takeda, Eiji Iwata, Natsuki Yonezawa, Yasumasa Kakei, Akiko Sakakibara, Masaya Akashi, Tsutomu Minamikawa, Takahide Komori

  Objective: The purpose of this study was to retrospectively investigate the multivariate relationships among the various risk factors for postoperative delirium in patients undergoing oral cancer surgery. Material and methods: The medical records of all patients with oral cancer who underwent curative head and neck surgery between April 2011 and March 2013 at our institution were retrospectively reviewed. There was a total of 188 patients, including 110 males and 78 females. Results: We found that older age, extensive surgical procedure, longer operation, excessive hemorrhage, blood transfusion, longer postoperative management in the intensive care unit, longer postoperative hospital stay, lower albumin level in the preoperative blood test, and lower total protein, albumi, n and hemoglobin levels and a higher C-reactive protein (CRP) level in the postoperative blood tests were significant variables in the univariate analysis (p < 0.05). We also determined that an older age (odds ratio [OR] = 6.83), intraoperative lower hemoglobin levels (OR = 6.82), and excessive hemorrhage (OR = 3.62) during surgery were significant variables in the multivariate analysis. Conclusion: Clinicians should pay special attention to preventing delirium during the postoperative management of older patients with these risk factors. Furthermore, increasing the hemoglobin levels during surgery may be able to prevent postoperative delirium. (C) 2015 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.

  CHURCHILL LIVINGSTONE, Sep. 2015, JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY, 43(7) (7), 1094 - 1098, English

  [Refereed]

  Scientific journal


• Sequential evaluation for bone union of transferred fibula flaps in reconstructed mandibles: panoramic X-ray versus computed tomography

  M. Akashi, K. Hashikawa, Y. Kakei, A. Sakakibara, T. Hasegawa, T. Minamikawa, T. Komori

  The purpose of this study was to sequentially evaluate bone union of fibular grafts in mandibular reconstruction. Patients who underwent routine follow-up computed tomography (CT) and panoramic X-ray imaging during a period of >= 2 years were enrolled. On panoramic X-ray images, bone union was scored as 0 (absent callus formation) or 1 (complete callus formation). On CT images, a scale of 0 to 2 was used (0, absent callus formation; 1, complete callus formation only on the labial side; 2, complete callus formation on both the labial and lingual side). A total of 56 bone junctions were evaluated in 20 patients. Five of 56 junctions (9%) in four of 20 patients (20%) showed radiological non-union (panoramic X-ray score = 0, CT score = 0 or 1) at 2 years after surgery. All bone junctions with radiological nonunion were located at the mandibular angle. No categorical values, including diabetes mellitus and radiation therapy, were significantly associated with radiological non-union. In conclusion, assessing at least two sides (i.e. labial and lingual sides) on CT images is adequate to evaluate bone union in transferred fibula flaps. Careful fixation at the mandibular angle may improve the rate of bone union.

  Corresponding, CHURCHILL LIVINGSTONE, Aug. 2015, INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 44(8) (8), 942 - 947, English

  [Refereed]

  Scientific journal


• Determining the location of the internal maxillary artery on ultrasonography and unenhanced magnetic resonance imaging before orthognathic surgery

  S. Arimoto, T. Hasegawa, N. Okamoto, A. Shioyasono, C. Tateishi, M. Akashi, H. Suzuki, S. Furudoi, T. Komori

  The purpose of this study was to examine the efficacy of ultrasonography (US) and unenhancedmagnetic resonance imaging(MRI) to determine the location of the internal maxillary artery (IMA) beforeorthognathic surgery. The study subjects were 19 patients (seven males and twelve females) with mandibular prognathismseen at the authors' institution between March 2012 and April 2013.The distance from the skin to the IMA (S-IMA)and the distance from the mandibular notch to the IMA (MN-IMA) were measured. Using the US and coronal MRI images, S-IMA(cl)and MN-IMA(cl) in the closed position and S-IMA(op) and MN-IMA(op) in the open position were measured at a total of four points in each cross-section. There were significant correlations between the distances measured on coronal MRI and US for all groups (P < 0.05). A total of 35 (92%) IMAs were classified as clear and three (8%) as unclear based on the US findings. Regarding the location of the IMA, 37 of the 38 sides studied (97%) were of the lateral type, while only one (3%) was of the medial type. The results of this study indicate that US can be used effectively to determine the location of the IMA.

  CHURCHILL LIVINGSTONE, Aug. 2015, INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 44(8) (8), 977 - 983, English

  [Refereed]

  Scientific journal


• Low-intensity pulsed ultrasound enhances bone morphogenetic protein expression of human mandibular fracture haematoma-derived cells

  W. Huang, T. Hasegawa, Y. Imai, D. Takeda, M. Akashi, T. Komori

  We previously demonstrated that human mandibular fracture haematoma-derived cells (MHCs) play an important role in mandibular fracture healing and that low-intensity pulsed ultrasound (LIPUS) accelerates this effect by stimulating various osteogenic cytokines. In the present study, we investigated how LIPUS affects the expression of bone morphogenetic proteins (BMPs), which are also known to have the ability to induce bone formation. MHCs were isolated from human mandibular fracture haematomas and the cells were divided into two groups: a LIPUS (+) group and a LIPUS (-) group, both of which were cultured in osteogenic medium. LIPUS was applied to the LIPUS (+) group 20 min a day for 4, 8, 14, and 20 days (1.5 MHz, 30 mW/cm(2)). Real-time PCR and immunofluorescence studies were carried out to determine the expression of BMP-2, 4, and 7. Compared to the LIPUS (+) group, gene expression levels were significantly increased in the LIPUS (+) group for BMP-2 on day 20 (67.38 +/- 26.59 vs. 11.52 +/- 3.42, P<0.001), for BMP-4 on days 14 (45.12 +/- 11.06 vs. 9.20 +/- 2.88, P=0.045) and 20 (40.96 +/- 24.81 vs. 3.22 +/- 1.53, P=0.035), and for BMP-7 on day 8 (48.11 +/- 35.36 vs. 7.03 +/- 3.96, P=0.034). These findings suggest that BMP-2, 4, and 7 may be mediated by LIPUS therapy during the bone repair process.

  CHURCHILL LIVINGSTONE, Jul. 2015, INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 44(7) (7), 929 - 935, English

  [Refereed]

  Scientific journal


• 歯科インプラント埋入のためのCT画像を用いた移植腓骨の形態評価

  Akashi Masaya, 橋川和信, 榊原晶子, 楠元順哉

  (一社)日本形成外科学会, Jun. 2015, 日本形成外科学会会誌, 35(6) (6), 359 - 359, Japanese


• Risk Factors Associated with Distant Metastasis in Patients with Oral Squamous Cell Carcinoma

  Takumi Hasegawa, Makiko Tanakura, Daisuke Takeda, Akiko Sakakibara, Masaya Akashi, Tsutomu Minamikawa, Takahide Komori

  Objective The purpose of this study was to retrospectively evaluate the incidence of distant metastasis (DM) after curative surgical treatment and assess the multivariate relationships among various risk factors for DM in patients with oral squamous cell carcinoma (OSCC). Study Design Case series with chart review. Setting Tertiary referral center. Subjects and Methods The medical records of all patients with OSCC without DM who underwent curative surgery with or without postoperative adjuvant chemoradiation between January 2001 and February 2014 at our institution were retrospectively reviewed. There were a total of 451 patients, including 271 men and 180 women, with a mean age of 65.9 13.5 years. Results Of these patients, 30 (6.7%) developed DM. During the follow-up period, the 5-year overall survival rate was 76.2%. Sites of DM comprised the lungs (80.0%), skin (13.3%), liver (10.0%), bone (6.7%), and brain (3.3%). We found the T classification and N classification as well as the histologic grade to be significant risk factors associated with the development of DM in the univariate analysis only (P = .003), whereas locoregional failure (odds ratio [OR], 4.03), multiple lymph node metastases (more than 4 positive nodes; OR, 3.32) and positive extracapsular spread (OR, 3.06) were identified to be significant risk factors associated with the development of DM in the multivariate analysis. Conclusion We demonstrated multivariate relationships among various risk factors for DM in OSCC patients. Clinicians should consider these risk factors and pay special attention to detecting DM early during the postoperative management of OSCC patients with these risk factors.

  SAGE PUBLICATIONS LTD, Jun. 2015, OTOLARYNGOLOGY-HEAD AND NECK SURGERY, 152(6) (6), 1053 - 1060, English

  [Refereed]

  Scientific journal


• Three-Dimensional Venous Visualization with Phase-Lag Computed Tomography Angiography for Reconstructive Microsurgery

  Shunsuke Sakakibara, Hiroyuki Onishi, Kazunobu Hashikawa, Masaya Akashi, Akiko Sakakibara, Tadashi Nomura, Hiroto Terashi

  Background Most free flap reconstruction complications involve vascular compromise. Evaluation of vascular anatomy provides considerable information that can potentially minimize these complications. Previous reports have shown that contrast-enhanced computed tomography is effective for understanding three-dimensional arterial anatomy. However, most vascular complications result from venous thromboses, making imaging of venous anatomy highly desirable. Methods The phase-lag computed tomography angiography (pl-CTA) technique involves 64-channel (virtually, 128-channel) multidetector CT and is used to acquire arterial images using conventional CTA. Venous images are three-dimensionally reconstructed using a subtraction technique involving combined venous phase and arterial phase images, using a computer workstation. Results This technique was used to examine 48 patients (12 lower leg reconstructions, 34 head and neck reconstructions, and 2 upper extremity reconstructions) without complications. The pl-CTA technique can be used for three-dimensional visualization of peripheral veins measuring approximately 1 mm in diameter. Conclusion The pl-CTA information was especially helpful for secondary free flap reconstructions in the head and neck region after malignant tumor recurrence. In such cases, radical dissection of the neck was performed as part of the first operation, and many vessels, including veins, were resected and used in the first free-tissue transfer. The pl-CTA images also allowed visualization of varicose changes in the lower leg region and helped us avoid selecting those vessels for anastomosis. Thus, the pl-CTA-derived venous anatomy information was useful for exact evaluations during the planning of free-tissue transfers.

  THIEME MEDICAL PUBL INC, May 2015, JOURNAL OF RECONSTRUCTIVE MICROSURGERY, 31(4) (4), 305 - 312, English

  [Refereed]

  Scientific journal


• Does Salivary Duct Repositioning Prevent Complications After Tumor Resection or Salivary Gland Surgery?

  Akiko Sakakibara, Tsutomu Minamikawa, Kazunobu Hashikawa, Shunsuke Sakakibara, Takumi Hasegawa, Masaya Akashi, Shungo Furudoi, Takahide Komori

  Purpose: Tissue that is resected for the treatment of oral tumors often includes salivary gland ducts. At their institution, the authors conserve and transfer as much of the salivary duct as possible during these procedures to avoid obstructive complications. Differentiating these obstructive complications from a metastatic node can be challenging and can confound subsequent oncologic management. This study compared and examined the effectiveness of salivary duct repositioning in decreasing the incidence of obstructive complications. Materials and Methods: Cases of oromandibular disease treated with salivary duct resection at Kobe University Graduate School of Medicine from 2008 to 2013 were retrospectively analyzed. Thirty-two cases (25 patients) of Wharton duct resection and 31 cases (31 patients) of Stensen duct resection were included. The incidence of complications after salivary duct repositioning, duct ligation, and retention of the sublingual gland around the Wharton duct was compared. Results: Wharton ducts were repositioned in 30 cases and ligated in 2 cases. Complications, including oral swelling at the Wharton duct, were observed in 5 cases of repositioning and 2 cases of ligation. Stensen ducts were repositioned in 9 cases and ligated in 22 cases. The only complication reported was a single case of salivary fistula after ligation. Conclusions: Salivary duct repositioning is performed to prevent blockage of physiologic salivary discharge. Complications were more frequently associated with Wharton ducts than with Stensen ducts because of the unique physiologic and anatomic characteristics of the Wharton duct. Repositioning of the salivary duct is a suitable method for preventing complications associated with the Wharton duct. (c) 2015 American Association of Oral and Maxillofacial Surgeons

  W B SAUNDERS CO-ELSEVIER INC, May 2015, JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 73(5) (5), 1003 - 1007, English

  [Refereed]

  Scientific journal


• 頸部郭清術後に中毒性巨大結腸症を併発した偽膜性腸炎を発症した1例

  岩田英治, Furudoi Shungo, 楠元順哉, Akashi Masaya, 後藤育子, Komori Takahide

  82歳男。左側口蓋部の腫瘤を指摘された。左側口蓋から左側上顎結節部にかけて25×15mm大の腫瘤を認め、その中心部に潰瘍を認めた。左側口蓋悪性腫瘍の診断で全身麻酔下に口蓋腫瘍切除術を施行した。摘出物の病理組織診断結果は、口蓋嚢胞腺癌であった。軽快退院となったが、外来通院中に撮影したCTにて右側頸部リンパ節に後発転移を認めた。全身麻酔下に右側根治的頸部廓清術変法を施行した。病理組織検査結果は転移が疑われるリンパ節は癌陽性および節外陰性、他リンパ節は癌陰性であった。SBT/ABPCを投与したが、炎症値の著しい上昇を認め、腹部に著明な膨満と軽度圧痛を認めた。CTにて著明な腸管拡張を認めた、中毒性巨大結腸症と診断した。バンコマイシンの経口投与に変更した。内視鏡的脱気術によるガス抜きや肛門バルーン留置による吸引にて、糞便貯留は改善した。

  (一社)日本口腔感染症学会, May 2015, 日本口腔感染症学会雑誌, 22(1) (1), 12 - 16, Japanese

  [Refereed]


• 当院における心臓血管外科患者に対する周術期口腔機能管理

  藤大補, Akashi Masaya, 古土井春吾, 西井美佳, 後藤育子, Komori Takahide

  (一社)日本口腔感染症学会, May 2015, 日本口腔感染症学会雑誌, 22(1) (1), 37 - 37, Japanese


• 抗RANKLモノクローナル抗体(デノスマブ)投与患者に発症した顎骨壊死の1例

  岩田英治, Akashi Masaya, Hasegawa Takumi, 古土井春吾, Komori Takahide

  (一社)日本口腔感染症学会, May 2015, 日本口腔感染症学会雑誌, 22(1) (1), 41 - 42, Japanese


• Postoperative abnormal response of C-reactive protein as an indicator for infectious complications after oral oncologic surgery with primary reconstruction

  Masaya Akashi, Shungo Furudoi, Kazunobu Hashikawa, Akiko Sakakibara, Takumi Hasegawa, Takashi Shigeta, Tsutomu Minamikawa, Takahide Komori

  Background: C-reactive protein (CRP) screening has been reported to be reliable for detection of infectious complications. Postoperative abnormal response of CRP can predict wound infection in colorectal surgery. This study aimed to determine the efficacy of CRP monitoring to detect infectious complications in oral oncologic surgery. Methods: One hundred patients who underwent oral cancer resection with primary reconstruction were enrolled. Postoperative kinetics of CRP were classified into a normal or abnormal response. Results: A normal CRP response after surgery was observed in 61 patients and an abnormal response was observed in 39. There were postoperative infectious complications in 21 patients, with surgical site infections in 13 patients (early onset in six and late onset in seven). Non-wound infections were found in nine patients. Sensitivity, specificity, the positive predictive value, and the negative predictive value for abnormal CRP response as a predictor for early infectious complications were 100%, 70.1%, 35.9%, and 100%, respectively. Conclusion: Postoperative serial CRP screening is a useful test as an indicator of infectious complications in oral oncologic surgery. Normal CRP responses can rule out almost all early infectious complications.

  BIOMED CENTRAL LTD, Apr. 2015, JOURNAL OF OTOLARYNGOLOGY-HEAD & NECK SURGERY, 44, 13, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Retrospective study of changes in the sensitivity of the oral mucosa: sagittal split ramus osteotomy (SSRO) versus intraoral vertical ramus osteotomy (IVRO)

  T. Hasegawa, C. Tateishi, M. Asai, Y. Imai, N. Okamoto, A. Shioyasono, A. Kimoto, M. Akashi, H. Suzuki, S. Furudoi, T. Komori

  We investigated changes in the sensitivity of cutaneous points and the oral mucosa after sagittal split ramus osteotomy (SSRO) and assessed the differences between SSRO and intraoral vertical ramus osteotomy (IVRO). The subjects included in this study were 46 patients with mandibular progmathism who underwent IVRO (88 rami) and 30 patients who underwent SSRO (59 rami). An objective evaluation of the neurosensory status of each patient was completed preoperatively and at 1, 4, 8, 12, and 24 weeks postoperatively. Other variables studied for each patient included sex, age, magnitude of mandibular setback, and amount of blood loss during surgery. We found that a neurosensory recovery occurred earlier in the oral mucosa than at cutaneous points. The number of oral mucosa points showing reduced neurosensory function and neurosensory disturbance after SSRO was significantly higher than after IVRO at 1, 4, and 8 weeks (P < 0.05). The nerve recovery observed after SSRO was delayed for a longer period than that noted in our previous study of IVRO. In conclusion, we found changes in sensitivity at cutaneous points and the oral mucosa after SSRO and assessed the differences between SSRO and IVRO.

  CHURCHILL LIVINGSTONE, Mar. 2015, INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 44(3) (3), 349 - 355, English

  [Refereed]

  Scientific journal


• 後方への進展なくルビエールリンパ節に転移を生じた口腔癌2例

  重田崇至, Minamikawa Tsutomu, Akashi Masaya, Hasegawa Takumi, 榊原晶子, 松井太輝, 筧康正, 高橋佑輔, 渋谷恭之, 梅田正博, Komori Takahide

  外側咽頭後リンパ節は後咽頭隙内で椎前筋の外側、内頸動脈の内側に位置しており、鼻副鼻腔、軟口蓋、上・中咽頭からリンパ流を受け内頸静脈に輸出リンパ管を送るリンパ節で、その最上方のものはルビエールリンパ節と称される。ルビエールリンパ節へは咽頭癌からの転移がしばしばみられるが、口腔癌の転移はまれとされている。ルビエールリンパ節に転移するような口腔癌への適切な治療方法は確立されておらず、その予後は極めて不良とされている。今回後方への浸潤がないにもかかわらずルビエールリンパ節に転移を生じた口腔癌2例を経験したので報告する。2例中1例は傍咽頭隙郭清を施行し、7年経過した現在無病生存で経過良好である。後方への浸潤のない口腔癌のルビエールリンパ節への転移機序、傍咽頭隙郭清の有用性について検討を行った。(著者抄録)

  (一社)日本口腔腫瘍学会, Mar. 2015, 日本口腔腫瘍学会誌, 27(1) (1), 1 - 6, Japanese

  [Refereed]


• The osteogenic activity of human mandibular fracture haematoma-derived progenitor cells is affected by bisphosphonate in vitro

  Y. Imai, T. Hasegawa, D. Takeda, M. Akashi, T. Komori

  It is known that bisphosphonates (BPs) suppress the activity of osteoclasts; however, it has not been reported whether BPs affect the potential of human mandibular fracture haematoma-derived cells (MHCs) for bone differentiation. In this study, we examined whether the degree of bone differentiation changes following the administration of BP in vitro. The effects of alendronate and risedronate (10(-8) to 10(-7) M (mol/l)) on cell proliferation were evaluated at 4 and 8 days, after which BP treatment was applied for 4, 8, 14, and 20 days prior to assessing the alkaline phosphatase (ALP) activity and performing the mineralization assay. Alendronate 10(-8) and 10(-7) M and risedronate 10(-7) M decreased the degree of cell proliferation on day 8 (P < 0.05). Using an ELISA, the ALP activity of the control, alendronate 10(-8) M, risedronate 10(-8) M, and risedronate 10(-7) M groups were 112.1 +/- 10.2%, 156.1 +/- 24.3%, 138.8 +/- 16.5%, and 133.3 +/- 10.3%, respectively, at 14 days after treatment (day 0 in each group was considered to be 100%). ALP activity was significantly higher in the alendronate 10(-8) M and risedronate 10(-8) and 10(-7) M groups than in the control group (P = 0.010, 0.014, and 0.009, respectively). It is possible that BPs increase the potential of MHCs for osteogenic differentiation depending on the concentration of the drug.

  CHURCHILL LIVINGSTONE, Mar. 2015, INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 44(3) (3), 412 - 416, English

  [Refereed]

  Scientific journal


• Clinical Study of 19 Cases Resulting in Dental Implant Removal.

  Hiroaki Suzuki, Daiki Takahashi, Kenji Matsuo, Akira Kimoto, Kousuke Matsumoto, Takumi Hasegawa, Masaya Akashi, Takahide Komori

  Dental implant treatment is a highly anticipated therapy with widespread use. However, various accidental symptoms and complications have been reported, and we may reluctantly have to remove an implant body out of necessity. This study evaluated 19 cases (21 jaws) that resulted in removal after the enforcement of implant treatment in our hospital from January 2003 to August 2013 and clarified the details of the removals clinically for the purpose of improvement of future treatment results. Implants were removed at a higher frequency in elderly patients, particularly those 70 years of age or older. The removal rate regarding the tooth missing style was high in edentulous jaw cases in both the maxilla and mandible, and was low in defective cases of middle teeth. Regarding the duration and cause of removal, approximately half of the implants were removed due to defective primary stability within 6 months; for periods greater than 6 months, all cases except 1 jaw were removed within 30 months and many cases were due to peri-implantitis. Preoperation evaluations, primary stability of the implant body at the time of the operation, long-term observation and maintenance, and patient education are crucial to reduce the frequency of removal.

  Kobe University School of Medicine, Feb. 2015, The Kobe journal of medical sciences, 61(4) (4), E102-8 - E108, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Prospective Study of Dental Intervention for Hematopoietic Malignancy

  K. Tsuji, Y. Shibuya, M. Akashi, S. Furudoi, K. Yakushijin, S. Kawamoto, A. Okamura, H. Matsuoka, T. Komori

  Various chemotherapeutic agents used in patients with hematopoietic malignancy cause serious side effects, including myelosuppression and immunosuppression. Immunosuppression makes patients more susceptible to infection, resulting in an increased risk of infectious complications, including the development of severe septicemia that may be life-threatening. It is necessary for dental staff to be familiar with an appropriate protocol in such cases and to share information about the chemotherapy with a hematologist. To verify the effectiveness of our dental intervention protocol, we conducted a prospective study on the incidence of complications for each myelosuppressive grade of chemotherapy in patients with hematopoietic malignancy. We compared the incidence of complications between treatment P (patients who finished all the dental treatments according to the protocol) and treatment Q (patients who did not) per grade (A, B, C, D) and incidence of systemic or oral findings. We also compared the incidence of oral complication related to the residual teeth between first chemo (patients who were undergoing chemotherapy for the first time) and prior chemo (not the first time). There were significant differences in inflammatory complications between treatment P and treatment Q. We found that both systemic and oral inflammatory complications increased with higher-grade myelosuppressive chemotherapy. Additionally, there was a significant difference between the incidence of oral complications related to the residual teeth between first chemo and prior chemo. Complete implementation of the dental intervention protocol was associated with fewer oral and systemic infectious and inflammatory complications in patients with hematopoietic malignancies undergoing chemotherapy. The incidence of oral and systemic complications also increased with grade of chemotherapy. These results support the validity of our dental intervention protocol. We should pay close attention to the oral state of de novo hematopoietic malignancy patients.

  SAGE PUBLICATIONS INC, Feb. 2015, JOURNAL OF DENTAL RESEARCH, 94(2) (2), 289 - 296, English

  Scientific journal


• Long-term Follow-up Study of Radial Forearm Free Flap Reconstruction After Hemiglossectomy

  Masaya Akashi, Kazunobu Hashikawa, Akiko Sakakibara, Takahide Komori, Hiroto Terashi

  Previous studies on postoperative long-term results in patients who underwent reconstructive free flap transfer following hemiglossectomy had some issues, including the heterogeneity of the patient population and the observation period. The present study aimed to evaluate changes of reconstructed tongues in patients who underwent radial forearmfree flap (RFFF) after hemiglossectomy with long-term follow-up. We enrolled 23 patients who underwent RFFFafter hemiglossectomy with a postoperative follow-up of 5 years or more. Postoperative status (eating, speech, sensation function) was assessed by concisemedical inquiries. Morphological changes of flaps were evaluated by reviewing clinical photographs. Hemiglossectomy involving the base of the tongue was performed in 4 cases (17.4%) and was limited to the mobile tongue in 19 cases (82.6%). The mean follow-up was 85.4 months (range, 60-122 months). All patients experienced gradually improved postoperative status. The most significant improvement was found between 1 and 5 years after surgery (P = 0.007), but not between 1 and 3 years (P = 0.075) or between 3 and 5 years (P = 0.530). In almost all of the flaps, there were few morphological changes throughout the follow-up period. Postoperative status in patients who underwent reconstructive RFFF following hemiglossectomy improved sequentially.

  LIPPINCOTT WILLIAMS & WILKINS, Jan. 2015, JOURNAL OF CRANIOFACIAL SURGERY, 26(1) (1), 48 - 51, English

  [Refereed]

  Scientific journal


• Spindle cell carcinoma of the oral cavity: The impact of chemotherapy on pulmonary metastatic tumor doubling time

  Takashi Shigeta, Tsutomu Minamikawa, Taiki Matsui, Yasumasa Kakei, Masaya Akashi, Takumi Hasegawa, Akiko Sakakibara, Yasuyuki Shibuya, Takahide Komori

  Spindle cell carcinoma (SpCC) an aggressive squamous cell carcinoma, variant, frequently metastasizes to regional lymph nodes and distant organs. Unfortunately, an effective treatment method for oral SpCC distant metastasis has not yet been established. Here we present 2 of oral SpCC cases that showed distant metastases after initial treatment, and 2 that showed distant metastases following during surgery. We calculated the tumor doubling time (TDT) and onset of the pulmonary metastatic and examined the TDT of the pulmonary metastatic tumor in patients with or without chemotherapy to determine the effect of anticancer drugs on oral SpCC. Tumor growth curves revealed that pulmonary metastasis likely grew to 1mm, 122 days before the initial examination, indicating that most oral SpCC patients should be treated for metastases. Three patients underwent chemotherapy for pulmonary metastatic tumor, complete response (CR) in one patient and no change (NC) in two. Thus, SpCC patients may have pulmonary micro-metastases even at the initial examination. We recommend wide resection for oral SpCC patients, followed by chemotherapy to prevent metastases.

  Kobe University School of Medicine, 2015, Kobe Journal of Medical Sciences, 61(3) (3), E64 - E70, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Measurement of the Total Number of Bacteria in Saliva Using Quantitative Real-Time PCR During Treatment for Head and Neck Malignancy: A Series of Cases

  GotoI, FurudoiS, Akashi Masaya, Komori Takahide

  2015, Oral Health Dent Manag, 142(776) (776), English

  [Refereed]

  Scientific journal


• Evaluation and comparison of CT values in bisphosphonate-related osteonecrosis of the jaw

  ImaiY, Hasegawa Takumi, TakedaD, KusumotoJ, Akashi Masaya, LeeSY, FurudoiS, Komori Takahide

  2015, J Oral Maxillofac Surg Med Pathol, English

  [Refereed]

  Scientific journal


• Alteration of Cell-Cell Junctions in Cultured Human Lymphatic Endothelial Cells with Inflammatory Cytokine Stimulation

  Yasumasa Kakei, Masaya Akashi, Takashi Shigeta, Takumi Hasegawa, Takahide Komori

  Background: To maintain normal function, the lymphatic endothelium is regulated by cell-cell junctions. There have been few studies of lymphatic endothelial cell junctions using standard cell biological methods. This study had two purposes: to characterize cell junctions in cultured lymphatic endothelial cells and to investigate the effects of the inflammatory cytokine TNF-alpha on altered cell-cell junctions. Methods and Results: Cultured human dermal lymphatic endothelial cells (HDLEC) were immunostained with the tight junction marker, ZO-1, and adherens junction markers, VE-cadherin and PECAM-1. In TNF-alpha-treated HDLEC, we evaluated changes in endothelial cell junctions by immunostaining and through the use of transendothelial electrical resistance (TER). Immunofluorescence staining of HDLEC revealed heterogeneity among the endothelial cell junctions, which could be classified into continuous and discontinuous junctions. In these cell junctions, ZO-1 and VE-cadherin were co-localized. Double immunofluorescence staining revealed the broad distribution of VE-cadherin at the cell periphery, where VE-cadherin and PECAM-1 were co-localized. TNF-alpha treatment decreased TER, caused a predominance in the appearance of discontinuous junctions with a reduction in the broad distribution of VE-cadherin at the cell periphery in HDLEC. Conclusions: The results indicate a heterogeneous distribution of cell junctions in HDLEC involving continuous and discontinuous junctions. Our data also suggest that TNF-alpha alters the normal distribution of cell junctions and affects the endothelial barrier of cultured lymphatic endothelial cells. The broad distribution of VE-cadherin at the cell periphery may reflect the lymphatic permeability.

  Corresponding, MARY ANN LIEBERT, INC, Sep. 2014, LYMPHATIC RESEARCH AND BIOLOGY, 12(3) (3), 136 - 143, English

  [Refereed]

  Scientific journal


• Sequential changes in oral dryness evaluated by a moisture-checking device in patients with oropharyngeal cancer during chemoradiotherapy: a pilot study

  Nishii M, Akashi Masaya, Kakei Y, Hasegawa Takumi, Minamikawa Tsutomu, Furudoi Shungo, Shibuya Yasuyuki, Takahashi M, Ohtsuki Naoki, Nibu Ken-ichi, Komori Takahide

  Corresponding, Jun. 2014, Oral Health Dent Manag, 13(2) (2), 507 - 11, English

  [Refereed]

  Scientific journal


• Oral squamous cell carcinoma with microscopic extracapsular spread in the cervical lymph nodes

  Y. Shibuya, Y. Ohtsuki, C. Hirai, T. Hasegawa, M. Akashi, T. Shigeta, T. Minamikawa, T. Komori

  The purpose of this study was to determine the detailed background of cases of oral squamous cell carcinoma (OSCC) with microscopic extracapsular spread (ECS) in the cervical lymph nodes. The cases of 78 patients with primary OSCC, who attended hospital from October 2007 to July 2011 and underwent resection of the primary tumour with neck dissection, were reviewed. The subjects were classified into three categories: pN0, pN+/ECS-, and pN+/ECS+; the outcomes of pN+/ECS+ patients were compared in detail with those of the other categories. Thirty-one cases (39.7%) were pN0, 25 cases (32.1%) were pN+/ECS-, and 22 cases (28.2%) were pN+/ECS+. The 3-year overall survival rate was 82.1% in pN0, 74.1% in pN+/ECS-, and 39.8% in pN+/ECS+ (pN0 vs. pN+/ECS+, P = 0.0004; pN+/ECS- vs. pN+/ECS+, P = 0.0086). The 3-year disease-specific survival rate was 96.2% in pN0, 77.2% in pN+/ECS-, and 39.8% in pN+/ECS+ (pN0 vs. pN+/ECS+, P < 0.0001; pN+/ECS- vs. pN+/ECS+, P = 0.0038). Patients with poorly differentiated carcinoma, those with three or more ECS+ nodes, and those with ECS+ node(s) located at levels III, IV, and V, had the worst prognosis among pN+/ECS+ subjects.

  CHURCHILL LIVINGSTONE, Apr. 2014, INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 43(4) (4), 387 - 392, English

  [Refereed]

  Scientific journal


• The osteogenic activity of human mandibular fracture haematoma-derived cells is stimulated by low-intensity pulsed ultrasound in vitro

  Y. Imai, T. Hasegawa, D. Takeda, M. Akashi, S. Y. Lee, T. Niikura, Y. Shibuya, M. Kurosaka, T. Komori

  Low intensity pulsed ultrasound (LIPUS) stimulation is a clinically established treatment method used to accelerate long bone fracture healing; however, this method is currently not applied to mandibular fractures. In this study, we investigated the effects of LIPUS on human mandibular fracture haematoma-derived cells (MHCs) in order to explore the possibility of applying LIPUS treatment to mandibular fractures. MHCs were isolated from five patients. The cells were divided into two groups: (1) LIPUS (+) group: MHCs cultured in osteogenic medium with LIPUS treatment; and (2) LIPUS () group: MHCs cultured in osteogenic medium without LIPUS treatment. The osteogenic differentiation potential and proliferation of the MHCs were compared between the two groups. The waveform used was equal to the wave conditions of a clinical fracture healing system. The gene expression levels of ALP, OC, Runx2, OSX, OPN, and PTH-R1 and mineralization were increased in the LIPUS (+) group compared to the LIPUS () group. There were no significant differences in cell proliferation between the two groups. These findings demonstrate the significant effects of LIPUS on the osteogenic differentiation of MHCs. This study provides significant evidence for the potential usefulness of the clinical application of LIPUS to accelerate mandibular fracture healing.

  CHURCHILL LIVINGSTONE, Mar. 2014, INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 43(3) (3), 367 - 372, English

  [Refereed]

  Scientific journal


• Changes in the sensitivity of cutaneous points and the oral mucosa after intraoral vertical ramus osteotomy

  T. Hasegawa, C. Tateishi, M. Asano, N. Takata, M. Akashi, T. Shigeta, S. Furudoi, Y. Shibuya, T. Komori

  In this study we investigated the changes in the sensitivity of cutaneous points and the oral mucosa that occur after intraoral vertical ramus osteotomy (IVRO). Additionally, postoperative changes in the sensitivity and the relationships between neurosensory disturbance and factors associated with IVRO operations were evaluated. An objective evaluation of the neurosensory status of cutaneous points and the oral mucosa of each patient was completed preoperatively and at 1, 2, 4, 8, 12, and 24 weeks postoperatively. The other variables studied for each patient included sex, age, magnitude of mandibular setback, and the amount of haemorrhage that occurred during surgery. In addition, the relationships between neurosensory disturbance and factors connected with IVRO operations were evaluated. We found that at cutaneous points, contributing factors such as sex, age, the magnitude of mandibular setback, and haemorrhage were associated with an increased risk of neurosensory disturbance after IVRO. However, these factors were not associated with that in the oral mucosa. In conclusion, we demonstrated the changes that occur in the sensitivity of cutaneous points and the oral mucosa after IVRO, the postoperative changes in sensitivity, and the relationships between neurosensory disturbance and factors connected with IVRO operations.

  CHURCHILL LIVINGSTONE, Nov. 2013, INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 42(11) (11), 1454 - 1461, English

  [Refereed]

  Scientific journal


• Preoperative MR angiography for free fibula osteocutaneous flap transfer

  Masaya Akashi, Tadashi Nomura, Shunsuke Sakakibara, Akiko Sakakibara, Kazunobu Hashikawa

  Introduction: Magnetic resonance angiography (MRA) is currently considered the most useful test to evaluate the vascular anatomy of the lower leg prior to free fibula osteocutaneous flap transfer. This study aimed to confirm the validity of preoperative MRA. Methods: In 19 patients underwent free fibula osteocutaneous flap transfer for maxillary and mandibular reconstruction, the MRA and intraoperative findings and the postoperative complications were retrospectively analyzed. The location and number of distal septocutaneous perforators (dSCPs) that were preoperatively identified and harvested with flaps were documented. Results: Preoperative MRA detected dSCPs with 100 % sensitivity. MRA findings also revealed the diversity of vascular structures, such as the tibio-peroneal bifurcation location and the anatomical relationship between the peroneal vessels and the fibula. No patients suffered postoperative ischemic complications in the donor leg. The total flap survival rate was 95 %. Conclusions: Preoperative MRA effectively excluded large vessel anomalies and peripheral vascular disease, and precisely identified the septocutaneous perforators. Additionally, preoperative MRA contributed to a safer fibular osteotomy by predicting the anatomical relationship between the peroneal vessels and the fibula. (c) 2013 Wiley Periodicals, Inc.

  WILEY-BLACKWELL, Sep. 2013, MICROSURGERY, 33(6) (6), 454 - 459, English

  [Refereed]

  Scientific journal


• Myelosuppression grading of chemotherapies for hematologic malignancies to facilitate communication between medical and dental staff: Lessons from two cases experienced odontogenic septicemia

  Masaya Akashi, Yasuyuki Shibuya, Junya Kusumoto, Shungo Furudoi, Yumiko Inui, Kimikazu Yakushijin, Atsuo Okamura, Hiroshi Matsuoka, Takahide Komori

  Background: Odontogenic diseases can be a risk factor for life-threatening infection in patients with hematologic malignancies during chemotherapy that induces myelosuppression of variable severity. Previous studies noted the necessity of the elimination of all odontogenic foci before hematopoietic stem cell transplantation. To enable planning for the adequate dental intervention, the oral medicine team must understand the general status of patient and the intensity of the chemotherapy, which is sometimes difficult to be fully appreciated by dental staff. Therefore, a simplified grading would facilitate the sharing of information between hematologists, dentists and oral hygienists. This study aimed to introduce our myelosuppression grading of chemotherapies for hematologic malignancies and analyze the timing of occurrence of severe odontogenic infection.Methods: 37 patients having received various chemotherapies for hematologic malignancies were enrolled. The chemotherapy regimens were classified into four grades based on the persistency of myelosuppression induced by chemotherapy. Mild myelosuppressive chemotherapies were classified as grade A, moderate ones as grade B, severe ones as grade C, and chemotherapies that caused severe myelosuppression and persistent immunodeficiency (known as conditioning regimens for transplant) as grade D. The timing of occurrence of severe odontogenic infection was retrospectively investigated.Results: Two patients (5.4%) had severe odontogenic infections after grade B or C chemotherapy. One occurred after extraction of non-salvageable teeth the other resulted from advanced periodontitis in a tooth that could not be extracted because of thrombocytopenia. Both were de novo hematologic malignancy patients. During grade D chemotherapy, no patients had severe odontogenic infections.Conclusions: The simplified grading introduced in this study is considered a useful tool for understanding the myelosuppressive state caused by chemotherapy and facilitating communication between medical and dental staff. During the period around the primary chemotherapy, especially for de novo hematologic malignancy patients who often received grade B to C myelosuppression chemotherapy, caution should be exercised for severe odontogenic infection by the oral medicine team, irrespective of whether invasive treatment is to be performed. © 2013 Akashi et al. licensee BioMed Central Ltd.

  Aug. 2013, BMC Oral Health, 13(1) (1), 41, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Risk factors associated with inferior alveolar nerve injury after extraction of the mandibular third molar-a comparative study of preoperative images by panoramic radiography and computed tomography

  T. Hasegawa, S. Ri, T. Shigeta, M. Akashi, Y. Imai, Y. Kakei, Y. Shibuya, T. Komori

  In this study we investigated the relationships among the risk factors for inferior alveolar nerve injury (IANI), and the difference between preoperative imaging findings on panoramic radiographs and computed tomography (CT), by univariate and multivariate analyses. We determined the following to be significant variables by multivariate analysis: panoramic radiographic signs, such as the loss of the white line of the inferior alveolar canal or the diversion of the canal; excessive haemorrhage during extraction; and a close relationship of the roots to the IAN (type 1 cases) on CT examination. CT findings of type 1 were associated with a significantly higher risk (odds ratio 43.77) of IANI. In addition, many panoramic findings were not consistent with CT findings (275 of 440 teeth; 62.5%). These results suggest that CT findings may be able to predict the development of IANI more accurately than panoramic findings. Panoramic radiography alone did not provide sufficiently reliable images required for predicting IANI. Therefore, when the panoramic image is suggestive of a close relationship between the impacted tooth and the IAN, CT should be recommended as a means of conducting further investigations.

  CHURCHILL LIVINGSTONE, Jul. 2013, INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 42(7) (7), 843 - 851, English

  [Refereed]

  Scientific journal


• がん切除後下顎骨再建における再建理論「CATコンセプト」 臨床症例における実践結果

  Hashikawa Kazunobu, Akashi Masaya, 野村 正, 寺師 浩人

  (一社)日本頭頸部癌学会, May 2013, 頭頸部癌, 39(2) (2), 166 - 166, Japanese

  [Refereed]


• Tight junction dysfunction in the stratum granulosum leads to aberrant stratum corneum barrier function in claudin-1-deficient mice

  Tomoko Sugawara, Noriko Iwamoto, Masaya Akashi, Taro Kojima, Junzo Hisatsune, Motoyuki Sugai, Mikio Furuse

  Background: Tight junctions (TJs) contribute to the epithelial barrier function by preventing leakage of solutes through the intercellular space. In the skin, TJs occur in the stratum granulosum (SG), where claudin-1 and claudin-4 are expressed as adhesion molecules of TJs. Claudin-1-deficient (Cldn1(-/-)) mice die within one day of birth accompanied by excessive transepidermal water loss, indicating a critical role of TJs in the epidermal barrier function. However, it has been debated whether the impaired TJ function in the SG also affects the stratum corneum (SC) barrier function or whether it results in skin barrier defects despite a normal SC barrier. Objective: To clarify whether the impaired TJ function affects the SC barrier function in Cldn1(-/-) mice. Methods: The morphology, barrier function and biochemical characteristic of the SC were compared between Cldn1(-/-) and Cldn(+/+) mice. Results: Scanning electron microscopy demonstrated abnormally wrinkled and rough corneocytes in Cldn1(-/-) mice. Notably, the X-gal tracer easily permeated into the Cldn1(-/-) SC, and water evaporation through isolated Cidn1(-/-) SC sheets was significantly higher than that through Cldn1(+/+) SC sheets. Furthermore, the ceramide composition of the SC lipids and filaggrin processing were altered in Cldn1(-/-) mice. Conclusion: Cldn1(-/-) mice exhibited the abnormal SC formation and SC barrier defects. These findings demonstrate for the first time that TJs in the SG play crucial roles in the complete SC formation and SC barrier function. (C) 2013 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.

  ELSEVIER IRELAND LTD, Apr. 2013, JOURNAL OF DERMATOLOGICAL SCIENCE, 70(1) (1), 12 - 18, English

  [Refereed]

  Scientific journal


• Oral Squamous Cell Carcinoma With Multiple Neck Metastases-Cases With More Than Ten Pathologically Positive Lymph Nodes in the Unilateral Side

  Yasuyuki Shibuya, Takumi Hasegawa, Masaya Akashi, Takashi Shigeta, Tsutomu Minamikawa, Takahide Komori

  Purpose: The purpose of this study is to know an outcome of the treatment for oral squamous cell carcinoma having more than 10 unilateral pathologically positive lymph nodes (pN)s. Patients and Methods: We reviewed 212 primary cases of oral squamous cell carcinoma that visited our hospital from January 1999 to December 2011 and underwent resection of the primary tumor with neck dissection. The patients were classified into 3 categories of pN0, pN1-9, and pN >= 10 cases, and an outcome of pN >= 10 was studied specifically in comparison with the other categories. Results: Of the patients studied, 103 cases (48.6%) were pN0, 103 cases (48.6%) were pN1-9, and 6 cases (2.8%) were pN >= 10. The pN >= 10 group, which had positive nodes of 10 to 30 on the ipsilateral side, was predominantly male and had no cases of well-differentiated squamous cell carcinoma. Extracapsular spread (ECS) was found in all pN >= 10 cases, and postoperative distant metastasis occurred in cases with ECS, which was localized in levels higher than III. The proportion of distant metastasis was 0% (0 cases) in pN0, 8.7% (9 cases) in pN1-9, and 66.7% (4 cases) in pN10. Overall survival rate in pN >= 10 was 20.8%, whereas it was 89.0% in pN0, and 34.9% in pN1-9. Conclusion: Oral squamous cell carcinoma having multiple neck metastases (pN >= 10) had a poor prognosis because the proportion of distant metastasis was higher in comparison with the other groups of pN0 and pN1-9. (C) 2013 American Association of Oral and Maxillofacial Surgeons J Oral Maxillofac Surg 71:793-797, 2013

  W B SAUNDERS CO-ELSEVIER INC, Apr. 2013, JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 71(4) (4), 793 - 797, English

  [Refereed]

  Scientific journal


• p16 overexpression in malignant and premalignant lesions of the oral and esophageal mucosa following allogeneic hematopoietic stem cell transplantation

  Yasumasa Kakei, Masaya Akashi, Hideki Komatsubara, Tsutomu Minamikawa, Takahide Komori

  Objectives: Secondary malignancy in the oral mucosa is recognized as one of the most serious complications in patients who received allogenic hematopoietic stem cell transplantation (HSCT). However, potential risk factors associated with carcinogenesis after HSCT that have been reported remain elusive. We experienced a rare case of secondary malignancies of the oral and esophageal mucosa and analyzed the expression of tumor suppressor gene product p16. Case report: A 35-year-old male had malignant lesions of the oral and esophageal mucosa two years after HSCT. Partial maxillectomy and endoscopic submucosal dissection were performed. Immunohistochemical analyses revealed that the tumor cells of malignant and premalignant lesions of the oral cavity and esophagus but not keratosis were positive for p16. Conclusions: Pathological examinations with p16 immunohistochemistry may contribute to an early diagnosis of secondary malignancy after HSCT.

  Corresponding, BIOMED CENTRAL LTD, Jun. 2012, HEAD & NECK ONCOLOGY, 4, 38, English

  [Refereed]

  Scientific journal


• A coronary artery disease-associated gene product, JCAD/KIAA1462, is a novel component of endothelial cell-cell junctions

  Masaya Akashi, Tomohito Higashi, Sayuri Masuda, Takahide Komori, Mikio Furuse

  Cell-cell junctioris play crucial roles in the organization and function of epithelial and endothelial cellular sheets. Here, we have identified the protein product for KIAA1462 gene, whose single nucleotide polymorphisms (SNPs) have recently reported to be associated with coronary artery disease, as a novel component of cell-cell junctions. We propose the name of KIAA1462 protein junctional protein associated with coronary artery disease (JCAD). JCAD is a similar to 145 kDa protein without any known domains but contains a proline-rich region. Immunolocalization studies revealed that JCAD is specifically localized at cell-cell junctions in endothelial cells but not in epithelial cells. The accumulation of JCAD at cell-cell junctions in cultured endothelial cells was impaired by RNAi-mediated suppression of VE-cadherin expression. In cell adhesion-deficient mouse L fibroblasts, JCAD was recruited to cell-cell contacts when cadherin-mediated cell-cell adhesion was induced. These results indicate that JCAD is a component of VE-cadherin-based cell-cell junctions in endothelial cells. This study also suggests the implication of endothelial cell-cell adhesion in coronary artery disease. (C) 2011 Elsevier Inc. All rights reserved.

  Lead, ACADEMIC PRESS INC ELSEVIER SCIENCE, Sep. 2011, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 413(2) (2), 224 - 229, English

  Scientific journal


• Phosphorylation state regulates the localization of Scribble at adherens junctions and its association with E-cadherin-catenin complexes

  Ken Yoshihara, Junichi Ikenouchi, Yasushi Izumi, Masaya Akashi, Shoichiro Tsukita, Mikio Furuse

  Mammalian ortholog of Scribble tumor suppressor has been reported to regulate cadherin-mediated epithelial cell adhesion by stabilizing the coupling of E-cadherin with catenins, but the molecular mechanism involved remains unknown. In this study, we investigated the relationship between the localization of mouse Scribble at cadherin-based adherens junctions (AJs) and its phosphorylation state. Immunofluorescence staining confirmed that Scribble was localized at AJs as well as at the basolateral plasma membrane in epithelial cells. We found that Scribble was detected as two bands by Western blotting analysis and that the band shift to the higher molecular weight was dependent on its phosphorylation at Ser 1601. Triton X-100 treatment extracted Scribble localized on the basolateral membrane but not Scribble localized at AJs in cultured epithelial cells, and the Triton X-100-resistant Scribble was the Ser 1601-unphosphorylated form. Conversely, an in-house-generated antibody that predominantly recognized Ser 1601-phosphorylated Scribble only detected Scribble protein on the lateral plasma membrane. Furthermore, Ser 1601-unphosphorylated Scribble was selectively coprecipitated with E-cadherin-catenin complexes in E-cadherin-expressing mouse L fibroblasts. Taken together, these results suggest that the phosphorylation state of Scribble regulates its complex formation with the E-cadherin-catenin system and may control cadherin-mediated cell-cell adhesion. (C) 2010 Elsevier Inc. All rights reserved.

  ELSEVIER INC, Feb. 2011, EXPERIMENTAL CELL RESEARCH, 317(4) (4), 413 - 422, English

  [Refereed]

  Scientific journal


• LSR defines cell corners for tricellular tight junction formation in epithelial cells

  Sayuri Masuda, Yukako Oda, Hiroyuki Sasaki, Junichi Ikenouchi, Tomohito Higashi, Masaya Akashi, Eiichiro Nishi, Mikio Furuse

  Epithelial cell contacts consist of not only bicellular contacts but also tricellular contacts, where the corners of three cells meet. At tricellular contacts, tight junctions (TJs) generate specialized structures termed tricellular TJs (tTJs) to seal the intercellular space. Tricellulin is the only known molecular component of tTJs and is involved in the formation of tTJs, as well as in the normal epithelial barrier function. However, the detailed molecular mechanism of how tTJs are formed and maintained remains elusive. Using a localization-based expression cloning method, we identified a novel tTJ-associated protein known as lipolysis-stimulated lipoprotein receptor (LSR). Upon LSR knockdown in epithelial cells, tTJ formation was affected and the epithelial barrier function was diminished. Tricellulin accumulation at the tricellular contacts was also diminished in these cells. By contrast, LSR still accumulated at the tricellular contacts upon tricellulin knockdown. Analyses of deletion mutants revealed that the cytoplasmic domain of LSR was responsible for the recruitment of tricellulin. On the basis of these observations, we propose that LSR defines tricellular contacts in epithelial cellular sheets by acting as a landmark to recruit tricellulin for tTJ formation.

  COMPANY OF BIOLOGISTS LTD, Feb. 2011, JOURNAL OF CELL SCIENCE, 124(4) (4), 548 - 555, English

  [Refereed]

  Scientific journal


• 上顎洞内異物が自然排出された1例

  Akashi Masaya, 李 進彰, 石田 佳毅, 中川 直美, 柳川 由美恵, 古森 孝英

  25歳女。歯科で左側上顎埋伏智歯の抜歯中に切削器具が破折し、洞内に迷入した。当日夜の当院初診時、パノラマX線で上顎洞後壁相当部に金属様不透過像を認めた。洞底線の不明瞭な部分を認めたが、明らかな交通はなかった。翌日再診時のCTでは、上顎洞内の異物は鼻腔側に移動していた。感染の可能性などを説明し、抜歯による疼痛や精神的疲労、本人・家族の希望もあり、同日全身麻酔下に異物除去術を施行した。歯肉頬移行部に横切開を加え、犬歯窩相当部の上顎洞前壁を開削し洞内へアプローチしたが、異物は発見できなかった。自然孔からの排出による誤飲誤嚥を疑い、術中に胸腹部X線撮影を行ったところ、胃内に金属様不透過像を認めた。外科・内科医の協力で、内視鏡下に胃の幽門部付近に停滞していた異物を摘出した。摘出物は長さ7mmの歯科用切削バーの破折片であった。術後合併症はなく、経過順調である。

  (公社)日本口腔外科学会, Mar. 2008, 日本口腔外科学会雑誌, 54(3) (3), 210 - 213, Japanese

  [Refereed]


• A clinical study of temporomandibular joint disorders -An analysis based on the Japanese subtype classification

  Yasuyuki Shibuya, Junichiro Takeuchi, Noriko Ikehata, Masaya Akashi, Tsuyoshi Fujita, Satoshi Yokoo, Masahiro Umeda, Takahide Komori

  In this study, cases of temporomandibular joint disorder (TMJD) were analyzed based on the subtype classification established by Japanese Society for the Temporomandibular Joint (JSTMJ) in 2001. The subject of our investigation consisted of cases who visited to the department of Oral and Maxillofacial Surgery of Kobe University Hospital in 2002 because of TMJD. Among them, any cases with no physical examination or strong psychogenic factors related to their symptoms were excluded. As a result, a total of 195 cases were investigated in this study. These cases consisted of 50 males and 145 females with a mean age of 38.1 years, and they were classified as type I (28 cases), type II (7 cases), type IIIa (91 cases), type IIIb (44 cases) and type IV (25 cases). The most frequently used therapy of the all diagnostic categories was a stabilization type of splint therapy (99 cases), and medication with muscle relaxants (52 cases) or analgesics (50 cases) ranked thereafter. All cases were followed until December 2004, and the results of the treatment were classified into 5 categories of 'improved', 'effective', 'no change', 'deteriorated' and 'discontinued'. No cases with a deterioration of symptoms were observed, while 109 cases (55.9%) were assessed as either 'improved' or 'effective'.

  2007, Kobe Journal of Medical Sciences, 53(2) (2), 63 - 70, English

  [Refereed]

  Scientific journal


• 上顎洞内異物が自然排出した1例

  Akashi Masaya, 李 進彰, 石田 佳毅, 中川 直美, 柳川 由美恵, 古森 孝英

  (NPO)日本口腔科学会, Jan. 2007, 日本口腔科学会雑誌, 56(1) (1), 189 - 190, Japanese


• 輸血拒否患者(エホバの証人)に対する口腔癌手術の経験

  UMEDA, Masahiro, 李進彰, 石田佳毅, 中川直美, 明石昌也, 大川修司, 西松成器, MINAMIKAWA,Tsutomu, KOMORI, Takahide

  エホバの証人は宗教上の理由から輸血を拒否し,比較的侵襲の大きな手術を予定する際にさまざまな問題を生じる可能性がある.今回われわれは,2名のエホバの証人患者に口腔癌手術を行ったので報告する.症例1は64歳女性の下顎歯肉癌(T2N2bM0)で,頸部郭清,下顎骨辺縁切除を施行した.出血量は300gであった.症例2は50歳男性の舌癌(T2N2bM0)で,頸部郭清,舌半側切除,大胸筋皮弁移植を施行した.出血量は445gであった.いずれも術前に患者と免責証書を取り交わし,手術は低血圧麻酔下で行われた.エホバの証人の治療に関する問題点についても考察を行った(著者抄録)

  (一社)日本口腔腫瘍学会, Mar. 2006, 日本口腔腫瘍学会誌, 18(1) (1), 1 - 5, Japanese

  [Refereed]

• 頭頸部腺様嚢胞癌に対する陽子線治療の初期成績

  瓜生 開人, 出水 祐介, 岩下 和真, 福光 延吉, 明石 昌也, 副島 俊典

  (一社)日本頭頸部癌学会, May 2024, 頭頸部癌, 50(2) (2), 190 - 190, Japanese


• Neutrophil-to-lymphocyte ratio in response to nivolumab for oral squamous cell carcinoma: a retrospective cohort study

  立浪秀剛, 冨原圭, 山田慎一, 池田篤司, 今上修一, 中井裕美, 黒原一人, 吉岡幸男, 長谷川巧実, 鳴瀬智史, 新山宗, 嶋根哲, 上田倫弘, 柳本惣市, 明石昌也, 梅田正博, 栗田浩, 宮崎晃亘, 新井直也, 野口誠

  2023, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 41st


• Oral and maxillofacial exfoliative cytology based on LBC/Oral Bethesda system: Roles of cytology at hospital dentistry.

  佐々木亜紀, 重岡学, 重岡学, 米田亜紀子, 米田亜紀子, 武田大介, 明石昌也

  2023, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 41st


• 頭頸部粘膜悪性黒色腫に対する陽子線治療+adjuvant Nivolumab療法

  瓜生開人, 出水祐介, 窪田光, 美馬正幸, 福光延吉, 今村善宣, 明石昌也, 副島俊典

  2023, 頭頸部癌, 49(2) (2)


• 嗅神経芽細胞腫に対する陽子線を用いた術後照射 神戸陽子線センターにおける初期治療成績

  瓜生 開人, 出水 祐介, 美馬 正幸, 福光 延吉, 明石 昌也, 副島 俊典

  (一社)日本頭頸部癌学会, May 2022, 頭頸部癌, 48(2) (2), 239 - 239, Japanese


• A study of patients who needed to apply for nursing insurance during hospitalization for oral cancer

  武田大介, 長谷川巧実, 筧康正, 明石昌也

  2022, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 40th


• A multicenter retrospective study of tongue cancer closed surgical margins-Japan Oral Oncology Group (JOOG) study-

  大鶴光信, 長谷川巧実, 明石昌也, 山川延宏, 桐田忠昭, 大倉正也, 大倉正也, 山田慎一, 栗田浩, 平井英治, 山本晃三, 猪俣徹, 里見貴史, 齋藤寛一, 野村武史, 古川浩平, 鳴瀬智史, 柳本惣市, 梅田正博

  2022, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 40th


• Tongue Osseous Choristoma in an 11-Year-Old Female: A Case Report and Literature Review Focusing on Pediatric Cases

  Satomi Arimoto, Manabu Shigeoka, Masaya Akashi

  Osseous choristoma is an uncommon benign lesion characterized by the presence of ectopic mature bone within soft tissue. In most cases, these lesions occur on the dorsum of the tongue in patients in their third and fourth decades of life. This article describes a case of lingual osseous choristoma in a pediatric patient. An eleven-year-old girl with a lingual mass was referred to our hospital from a dental clinic. Total excisional biopsy and histological examination were performed, and osseous choristoma was diagnosed. The postoperative course was uneventful with no signs of recurrence during the 12 months after surgery. Moreover, a literature review focusing on pediatric cases with lingual osseous choristoma was performed to know the etiology, clinicopathological characteristics, and course of treatment of the lesion.

  Hindawi Limited, 2021, Case Reports in Dentistry, 2021, English

  Book review


• Effect of percutaneous administration of carbon dioxide to improve tumor immunosuppression

  八谷奈苗, 長谷川巧実, 武田大介, 齊藤泉, 有本智美, 筧康正, 榊原晶子, 明石昌也

  2021, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 39th


• A case of tongue cancer histopathologically diagnosed as lymphoepithelial carcinoma

  武田大介, 重岡学, 長谷川真子, 村上明希, 八谷奈苗, 長谷川巧実, 明石昌也

  2021, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 39th


• Consideration of non-survival cases in patients with oral squamous cell carcinoma and Future perspectives in a tertiary referral center

  長谷川巧実, 武田大介, 筧康正, 明石昌也

  2021, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 39th


• 消化器外科SSI対策 経験からその検証へ 大腸癌術後SSIに対する周術期口腔ケアの予防効果に関する多施設共同研究

  延原 浩, 五月女 さき子, 林田 咲, 梅田 正博, 長谷川 巧実, 明石 昌也, 山田 慎一, 栗田 浩, 中原 寛和, 上田 順宏, 桐田 忠昭, 中村 知寿, 渋谷 恭之, 山口 泰平, 森 和代

  (一社)日本外科感染症学会, Oct. 2020, 日本外科感染症学会雑誌, 17(5) (5), 329 - 329, Japanese


• 舌下神経麻痺を契機に発見された前立腺癌脳転移の1例

  佐々木 亜紀, 木本 明, 八谷 奈苗, 村上 明希, 明石 昌也

  (NPO)日本口腔科学会, Sep. 2020, 日本口腔科学会雑誌, 69(3) (3), 245 - 245, Japanese


• 舌に生じた腺扁平上皮癌の1例

  大堀 浩明, 筧 康正, 山下 淳也, 南川 勉, 明石 昌也

  (NPO)日本口腔科学会, Sep. 2020, 日本口腔科学会雑誌, 69(3) (3), 248 - 248, Japanese


• 顎骨壊死の病態機序解明

  明石 昌也

  顎骨壊死(osteonecrosis of the jaws)とは、様々な誘因により発症する顎口腔領域において最も難治性の感染性病変である。顎骨壊死の主な誘因は骨吸収抑制薬と放射線で、双方ともに抜歯や義歯性の潰瘍といった外的刺激が契機となることが多いが、歯周炎等の歯性感染症により続発して自然発症するケースもある。病態機序の解明はいまだ十全ではないものの、著者らがこれまで行なってきた研究結果について概略する。(著者抄録)

  (株)北隆館, Jul. 2020, BIO Clinica, 35(7) (7), 680 - 683, Japanese


• 頭頸部癌における免疫チェックポイント阻害薬後の救済化学療法についての後方視的検討

  小山 泰司, 清田 尚臣, 今村 善宣, 長谷 善明, 高倉 嗣丈, 蓼原 瞬, 入谷 啓介, 古川 竜也, 手島 直則, 四宮 弘隆, 宮脇 大輔, 明石 昌也, 佐々木 良平, 丹生 健一

  (一社)日本頭頸部癌学会, Jul. 2020, 頭頸部癌, 46(2) (2), 174 - 174, Japanese


• 当院における頭頸部がんに対する高用量シスプラチン併用化学放射線療法による急性腎障害の後方視的検討

  高倉 嗣丈, 今村 善宣, 小山 泰司, 長谷 善明, 四宮 弘隆, 手島 直則, 古川 竜也, 入谷 啓介, 蓼原 瞬, 宮脇 大輔, 明石 昌也, 佐々木 良平, 丹生 健一, 清田 尚臣

  (一社)日本頭頸部癌学会, Jul. 2020, 頭頸部癌, 46(2) (2), 185 - 185, Japanese


• 嚢胞壁の一部に異所性胃粘膜を認めた甲状舌管嚢胞の1例

  小林 正樹, 森田 麻希, 中村 健人, 中村 知寿, 水谷 友美, 菱田 純代, 青木 尚史, 加藤 伸一郎, 明石 昌也, 渋谷 恭之

  (NPO)日本口腔科学会, Jul. 2020, 日本口腔科学会雑誌, 69(2) (2), 87 - 87, Japanese


• 原因検索に難渋した口底蜂窩織炎の1例

  岩田 英治, 橘 進彰, 格谷 僚, 高井 美玲, 榎本 由依, 楠元 順哉, 高田 直樹, 明石 昌也

  (NPO)日本口腔科学会, Jul. 2020, 日本口腔科学会雑誌, 69(2) (2), 119 - 120, Japanese


• 顎骨壊死を契機として切開排膿を要した咀嚼筋隙膿瘍の検討

  下間 祐輝, 武田 大介, 佐々木 亜紀, 小林 英里奈, 八橋 明子, 齋藤 泉, 楠元 順哉, 長谷川 巧実, 古土井 春吾, 明石 昌也

  (NPO)日本口腔科学会, Jul. 2020, 日本口腔科学会雑誌, 69(2) (2), 121 - 121, Japanese


• 当科で歯科介入を行った高用量骨吸収抑制薬使用患者におけるMRONJ発症とリスク因子についての検討

  廣田 純也, 長谷川 巧実, 武田 大介, 筧 康正, 楠元 順哉, 榊原 晶子, 木本 明, 明石 昌也

  (NPO)日本口腔科学会, Jul. 2020, 日本口腔科学会雑誌, 69(2) (2), 153 - 153, Japanese


• 神戸大学医学部付属病院歯科口腔外科における抗菌薬適正使用の現状

  吉位 亮助, 楠元 順哉, 松村 恵美, 古土井 春吾, 明石 昌也

  (一社)日本口腔感染症学会, Jul. 2020, 日本口腔感染症学会雑誌, 27(1) (1), 36 - 37, Japanese


• エピシル口腔用液による口腔粘膜炎の疼痛緩和についての検討

  松村 恵実, 西井 美佳, 楠元 順哉, 古土井 春吾, 明石 昌也

  (一社)日本口腔感染症学会, Jul. 2020, 日本口腔感染症学会雑誌, 27(1) (1), 38 - 38, Japanese


• 若年女性の頬部蜂窩織炎から肺化膿症にまで進展したMRSA菌血症の1例

  大橋 雄高, 南川 勉, 岩田 英治, 松村 恵美, 古土井 春吾, 明石 昌也

  (一社)日本口腔感染症学会, Jul. 2020, 日本口腔感染症学会雑誌, 27(1) (1), 41 - 41, Japanese


• 頬部の皮膚充填剤により生じた頬部蜂窩織炎の1例

  榎本 由依, 橘 進彰, 岩田 英治, 高田 直樹, 明石 昌也

  (一社)日本口腔感染症学会, Jul. 2020, 日本口腔感染症学会雑誌, 27(1) (1), 44 - 44, Japanese


• 症状の背景にあるもの

  明石 昌也

  (一社)兵庫県歯科医師会, Jun. 2020, 歯界月報, (827) (827), 24 - 30, Japanese


• 口腔癌に対するレーザー照射とその影響

  佐々木 崇良, 木本 明, 八谷 奈苗, 山下 淳也, 重岡 学, 明石 昌也

  (一社)日本レーザー歯学会, May 2020, 日本レーザー歯学会誌, 30(1) (1), 22 - 22, Japanese


• アルツハイマー型認知症患者の口腔内に生じた孤立性形質細胞腫に対して放射線治療が奏功した1例

  棚倉万紀子, 武田大介, 三谷泉, 明石昌也

  2020, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 38th


• 口腔外科手術周術期における抗血栓療法患者に対するヘパリンブリッジの有効性に関する多施設共同後ろ向き観察研究

  後藤弘一, 山田慎一, 長谷川巧実, 吉村仁志, 五月女さき子, 冨原圭, 長谷川温, 菱田純代, 上田順宏, 傳田祐也, 岡本健二郎, 明石昌也, 近津大地, 野口誠, 太田嘉英, 藤田茂之, 藤田茂之, 渋谷恭之, 桐田忠昭, 梅田正博, 栗田浩

  2020, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 38th


• Effect of perioperative oral management on the prevention of surgical site infection after colorectal cancer surgery: A multicenter retrospective analysis

  延原浩, 五月女さき子, 林田咲, 梅田正博, 長谷川巧実, 明石昌也, 山田慎一, 栗田浩, 中原寛和, 上田順宏, 桐田忠昭, 中村知寿, 渋谷恭之, 山口泰平, 森和代

  2020, 日本外科感染症学会雑誌(Web), 17(5) (5)


• 【「顎骨再建材料」】ポリ-L-乳酸

  明石 昌也

  (一社)日本歯科理工学会, Jan. 2020, 日本歯科理工学会誌, 39(1) (1), 13 - 16, Japanese


• 明日から役立つ!がん患者の口腔機能管理テクニック

  西井 美佳, 古土井 春吾, 松村 恵実, 楠元 順哉, 明石 昌也

  (一社)日本口腔感染症学会, Jan. 2020, 日本口腔感染症学会雑誌, 26(2) (2), 68 - 73, Japanese


• 舌に発生した粘液脂肪腫の1例

  岩田 英治, 橘 進彰, 高井 美玲, 榎本 由依, 楠元 順哉, 高田 直樹, 明石 昌也

  粘液脂肪腫は極めて稀な脂肪腫の亜型であり、本邦でこれまで報告された口腔内の発生例はわずか4例しかない。われわれは64歳男性の舌縁部に発生した粘液脂肪腫の1例を経験したので報告する。患者は舌の腫脹を主訴に紹介初診された。T1、T2強調像にて11×9×11mmの高信号を認めた。全身麻酔下に舌腫瘍摘出術を行った。病理組織学的所見は、成熟した大小不同の脂肪細胞の増殖からなり、間質に粘液腫様変化を認めたため、舌粘液脂肪腫と診断した。術後1年が経過するが再発は認めていない。(著者抄録)

  (一社)日本口腔腫瘍学会, Dec. 2019, 日本口腔腫瘍学会誌, 31(4) (4), 203 - 206, Japanese


• 重症心身障がい児(者)における口腔衛生状態の実態調査

  柴田 里紗, 明石 昌也, 小紫 香代, 玉村 宣尚

  国立病院総合医学会, Nov. 2019, 国立病院総合医学会講演抄録集, 73回, P1 - 805, Japanese


• 【「歯科用レーザーの現在」】軟組織に対する歯科用レーザーの活用

  木本 明, 明石 昌也, 古森 孝英

  (一社)日本歯科理工学会, Sep. 2019, 日本歯科理工学会誌, 38(3) (3), 149 - 152, Japanese


• 歯科、口腔外科領域における進歩と課題 口腔がんに対するレーザー治療の応用 早期表在性舌がんに対するレーザー治療

  木本 明, 明石 昌也

  (NPO)日本レーザー医学会, Sep. 2019, 日本レーザー医学会誌, 40(3) (3), 265 - 265, Japanese


• 多剤耐性菌が検出された薬剤関連顎骨壊死患者の治療経験

  大橋 雄高, 古土井 春吾, 岸本 恵, 岩田 英治, 高橋 淳子, 楠元 順哉, 明石 昌也, 古森 孝英

  (一社)日本口腔感染症学会, Jun. 2019, 日本口腔感染症学会雑誌, 26(1) (1), 43 - 43, Japanese


• ゾレドロン酸の投与が奏効したび漫性硬化性下顎骨骨髄炎の1例

  佐藤 匠, 古土井 春吾, 大橋 雄高, 岸本 恵美, 岩田 英治, 楠元 順哉, 明石 昌也, 古森 孝英

  (一社)日本口腔感染症学会, Jun. 2019, 日本口腔感染症学会雑誌, 26(1) (1), 45 - 46, Japanese


• 頭頸部癌に対する放射線治療後の放射線性顎骨壊死に関する検討

  窪田 光, 宮脇 大輔, 佐々木 香織, 妹尾 悟史, 井上 由子, 川口 弘毅, 西川 遼, 石原 武明, 吉田 賢史, 岸本 恵美, 明石 昌也, 佐々木 良平

  (一社)日本頭頸部癌学会, May 2019, 頭頸部癌, 45(2) (2), 244 - 244, Japanese


• 頭頸部癌に対する放射線治療後の放射線性顎骨壊死に関する検討

  窪田 光, 宮脇 大輔, 佐々木 香織, 妹尾 悟史, 井上 由子, 川口 弘毅, 西川 遼, 石原 武明, 吉田 賢史, 岸本 恵美, 明石 昌也, 佐々木 良平

  (一社)日本頭頸部癌学会, May 2019, 頭頸部癌, 45(2) (2), 244 - 244, Japanese


• 口腔扁平上皮癌における人工多能性幹細胞関連因子の発現について

  武田大介, 長谷川巧実, 江崎友美, 榊原晶子, 明石昌也, 南川勉, 古森孝英

  2019, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 37th


• 当科におけるUnusualな再発症例について

  筧康正, 南川勉, 松田彩, 榊原晶子, 長谷川巧実, 明石昌也, 古森孝英

  2019, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 37th, 122, Japanese


• 炭酸ガスを使用した効率的な低酸素環境改善による口腔癌の新規治療法の開発と治療抵抗性の改善効果

  長谷川巧実, 武田大介, 齋藤泉, 岩田英治, 筧康正, 榊原晶子, 明石昌也, 南川勉, 古森孝英

  2019, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 37th, 120, Japanese


• 再建を伴う口腔癌の周術期静脈血栓塞栓症について

  筧康正, 明石昌也, 長谷川巧実, 南川勉, 古森孝英

  2019, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 37th, 112, Japanese


• 口腔癌におけるPET‐MRI~第二報~

  瓜生開人, 南川勉, 大橋雄高, 大橋雄高, 松田彩, 武田大介, 筧康正, 松井太輝, 榊原晶子, 長谷川巧実, 明石昌也, 古森孝英

  2019, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 37th, 156, Japanese


• 薬剤関連顎骨壊死の転帰に影響する因子についての検討

  古土井 春吾, 岩田 英治, 岸本 恵美, 高橋 淳子, 楠元 順哉, 明石 昌也, 古森 孝英

  当科で経験した薬剤関連顎骨壊死症例65例(男性15例、女性50例、平均74.9歳)を分析し、転帰に影響する因子について検討した。原疾患別では、悪性腫瘍が23例(35.4%)、骨粗鬆症が39例(60.0%)、悪性腫瘍・骨粗鬆症合併が3例(4.6%)であった。治療法はnonsurgical approach群が35例(53.8%)、conservative surgery群が23例(35.4%)、extensive surgery群が7例(10.8%)であった。転帰については、「症状消失」が38例(58.5%)、Stageが下がった「症状改善」が11例(16.9%)、「症状不変」が7例(10.8%)、Stageが上昇した「症状悪化」は5例(7.7%)、経過観察中に患者が原疾患により死亡した症例が4例(6.3%)であった。原疾患別とリスク製剤休薬・変更の可否で統計学的に有意差が認められ、悪性腫瘍と休薬・変更不可症例では「症状消失」症例が有意に少なかった。また、各Stageに占める「症状消失」症例の割合をみたところ、Stage 0:8/10例(80.0%)、Stage 1:14/17例(82.4%)、Stage 2:14/29例(48.3%)、Stage 3:2/5例(40.0%)であった。

  (一社)日本口腔感染症学会, Dec. 2018, 日本口腔感染症学会雑誌, 25(2) (2), 71 - 77, Japanese


• 当科におけるインプラント埋入症例と喪失リスク因子に関する後ろ向き観察研究

  長谷川 巧実, 武田 大介, 齋藤 泉, 有本 智美, 筧 康正, 明石 昌也, 鈴木 泰明, 古森 孝英

  (公社)日本顎顔面インプラント学会, Nov. 2018, Japanese Journal of Maxillo Facial Implants, 17(3) (3), 224 - 224, Japanese


• 当科におけるインプラント埋入症例と喪失リスク因子に関する後ろ向き観察研究

  長谷川巧実, 武田大介, 齋藤泉, 有本智美, 筧康正, 明石昌也, 鈴木泰明, 古森孝英

  (公社)日本顎顔面インプラント学会, Nov. 2018, Japanese Journal of Maxillo Facial Implants, 17(3) (3), 224 - 224, Japanese


• Four‐dimensional CT(4DCT)による咀嚼時の下顎頭前方滑走の解析

  関谷俊範, 明石昌也, 筧康正, 根宜典行, 西尾卓郎, 星野貴志, 櫻井玲, 泉谷信行, 日下亜起子, 古森孝英, 村上卓道

  (公社)日本診療放射線技師会, Sep. 2018, 日本診療放射線技師会誌, 65(9) (9), 1011 - 1011, Japanese


• 人工呼吸器装着中の重症心身障害児者における口腔環境と肺炎の関係

  柴田 里紗, 明石 昌也, 玉村 宜尚

  日本重症心身障害学会, Aug. 2018, 日本重症心身障害学会誌, 43(2) (2), 343 - 343, Japanese


• 当院における過去10年間の外傷歯症例の臨床的検討―世代別の特徴に関する分析―

  佐藤匠, 古土井春吾, 可信雅彦, 筧康正, 明石昌也, 古森孝英

  (一社)日本外傷歯学会, Jul. 2018, 日本外傷歯学会総会・学術大会プログラム・抄録集, 18回, 48 - 48, Japanese


• 頸部リンパ節転移陽性口腔扁平上皮癌の予後因子におけるlymph node ratioの有用性に関する検討

  筧 康正, 南川 勉, 白井 達也, 大橋 雄高, 松田 彩, 松井 太輝, 榊原 晶子, 長谷川 巧実, 明石 昌也, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2018, 日本口腔科学会雑誌, 67(2) (2), 103 - 103, Japanese


• 頸部郭清を伴う口腔癌術後合併症に関する臨床的検討

  八谷 奈苗, 長谷川 巧実, 武田 大介, 筧 康正, 松井 太輝, 明石 昌也, 南川 勉, 古土井 春吾, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2018, 日本口腔科学会雑誌, 67(2) (2), 117 - 117, Japanese


• 膠原病を有する口腔癌症例の臨床的検討

  廣田 純也, 南川 勉, 松田 彩, 松井 太輝, 筧 康正, 榊原 晶子, 長谷川 巧実, 明石 昌也, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2018, 日本口腔科学会雑誌, 67(2) (2), 121 - 121, Japanese


• 当科における口腔癌への緩和照射に関する臨床的検討

  松田 彩, 南川 勉, 白井 達也, 筧 康正, 松井 太輝, 長谷川 巧実, 榊原 晶子, 明石 昌也, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2018, 日本口腔科学会雑誌, 67(2) (2), 112 - 112, Japanese


• 術後に気道閉塞を生じた口腔癌患者の3例

  香西 慎一, 松井 太輝, 筧 康正, 松田 彩, 明石 昌也, 長谷川 巧実, 南川 勉, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2018, 日本口腔科学会雑誌, 67(2) (2), 113 - 113, Japanese


• 口腔癌術後に予定外に再度の全身麻酔による手術を要した症例の検討

  大橋 雄高, 筧 康正, 松田 彩, 松井 太輝, 榊原 晶子, 長谷川 巧実, 明石 昌也, 南川 勉, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2018, 日本口腔科学会雑誌, 67(2) (2), 110 - 110, Japanese


• 口腔癌原発巣手術における術中迅速診断の臨床的検討

  白井 達也, 松井 太輝, 筧 康正, 大橋 雄高, 松田 彩, 長谷川 巧実, 明石 昌也, 南川 勉, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2018, 日本口腔科学会雑誌, 67(2) (2), 119 - 119, Japanese


• 当科における過去10年間の血液培養検査に関する臨床的検討

  大橋 雄高, 古土井 春吾, 岸本 恵, 岩田 英治, 高橋 淳子, 楠元 順哉, 明石 昌也, 古森 孝英

  (一社)日本口腔感染症学会, Jun. 2018, 日本口腔感染症学会雑誌, 25(1) (1), 48 - 48, Japanese


• 翼突下顎隙に及ぶ蜂窩織炎に付随した舌神経知覚鈍麻に対し星状神経節ブロックを施行した1例

  白井 達也, 明石 昌也, 岸本 恵実, 古森 孝英

  (NPO)日本口腔科学会, Mar. 2018, 日本口腔科学会雑誌, 67(1) (1), 64 - 64, Japanese


• 下顎智歯抜歯後の術後出血に関するリスク因子についての検討

  廣田純也, 長谷川巧実, 西條翔, 大山剛平, 武田大介, 明石昌也, 古森孝英

  2018, 日本口腔科学会雑誌(Web), 67(2) (2)


• 頸部郭清を伴う口腔癌術後合併症に関する臨床的検討

  八谷奈苗, 長谷川巧実, 武田大介, 筧康正, 松井太輝, 明石昌也, 南川勉, 古土井春吾, 古森孝英

  2018, 日本口腔科学会雑誌(Web), 67(2) (2), 117(J‐STAGE), Japanese


• 頸部リンパ節転移陽性口腔扁平上皮癌の予後因子におけるlymph node ratioの有用性に関する検討

  筧康正, 南川勉, 白井達也, 大橋雄高, 大橋雄高, 松田彩, 松井太輝, 榊原晶子, 長谷川巧実, 明石昌也, 古森孝英

  2018, 日本口腔科学会雑誌(Web), 67(2) (2), 103(J‐STAGE), Japanese


• 膠原病を有する口腔癌症例の臨床的検討

  廣田純也, 南川勉, 松田彩, 松井太輝, 筧康正, 榊原晶子, 長谷川巧実, 明石昌也, 古森孝英

  2018, 日本口腔科学会雑誌(Web), 67(2) (2), 121(J‐STAGE), Japanese


• 放射線性下顎骨壊死の診断におけるfollow up PET‐CTの有用性

  鰐渕聡, 明石昌也, 平岡佑二郎, 武田大介, 筧康正, 松井太輝, 長谷川巧実, 南川勉, 古土井春吾, 古森孝英

  2018, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 36th, 161, Japanese


• 皮弁再建後の再発舌癌に対して救済手術を施行した2例

  大橋雄高, 松井太輝, 筧康正, 正井友里子, 松田彩, 榊原晶子, 長谷川巧実, 明石昌也, 南川勉, 古森孝英

  2018, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 36th, 181, Japanese


• 当科における口腔癌への緩和照射に関する臨床的検討

  松田彩, 南川勉, 白井達也, 筧康正, 松井太輝, 長谷川巧実, 榊原晶子, 明石昌也, 古森孝英

  2018, 日本口腔科学会雑誌(Web), 67(2) (2), 112(J‐STAGE), Japanese


• 超音波断層法による口腔癌頸部リンパ節転移の検討

  筧康正, 南川勉, 正井友里子, 松田彩, 松井太輝, 榊原晶子, 長谷川巧実, 明石昌也, 重田崇至, 古森孝英

  2018, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 36th, 160, Japanese


• 舌扁平上皮癌の原発巣再発例の検討

  松井太輝, 南川勉, 大橋雄高, 正井友里子, 松田彩, 筧康正, 長谷川巧実, 明石昌也, 古森孝英

  2018, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 36th, 164, Japanese


• 術後に気道閉塞を生じた口腔癌患者の3例

  香西慎一, 松井太輝, 筧康正, 松田彩, 明石昌也, 長谷川巧実, 南川勉, 古森孝英

  2018, 日本口腔科学会雑誌(Web), 67(2) (2), 113(J‐STAGE), Japanese


• 口腔癌術後病理minor risk症例の臨床的検討

  松田彩, 松井太輝, 正井友里子, 筧康正, 長谷川巧実, 榊原晶子, 明石昌也, 南川勉, 古森孝英

  2018, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 36th, 151, Japanese


• 口腔癌術後に予定外に再度の全身麻酔による手術を要した症例の検討

  大橋雄高, 筧康正, 松田彩, 松井太輝, 榊原晶子, 長谷川巧実, 明石昌也, 南川勉, 古森孝英

  2018, 日本口腔科学会雑誌(Web), 67(2) (2), 110(J‐STAGE), Japanese


• 口腔癌再建術後Recipient‐site infectionの定義に関する文献レビュー

  明石昌也, 筧康正, 武田大介, 松井太輝, 榊原晶子, 長谷川巧実, 南川勉, 橋川和信, 古森孝英

  2018, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 36th, 149, Japanese


• 口腔癌原発巣手術における術中迅速診断の臨床的検討

  白井達也, 松井太輝, 筧康正, 大橋雄高, 大橋雄高, 松田彩, 長谷川巧実, 明石昌也, 南川勉, 古森孝英

  2018, 日本口腔科学会雑誌(Web), 67(2) (2), 119(J‐STAGE), Japanese


• 上顎部分切除後の顎義歯装着に関する皮弁再建・非再建群の比較

  可信雅彦, 筧康正, 明石昌也, 古森孝英

  (一社)日本顎顔面補綴学会, Dec. 2017, 顎顔面補綴, 40(2) (2), 55 - 55, Japanese


• 移植腓骨へ埋入したインプラント体の周囲炎に対し炭酸ガスレーザーを適用した1例

  山下淳也, 鈴木泰明, 筧康正, 木本明, 長谷川巧実, 明石昌也, 松本耕佑, 古森孝英

  (公社)日本顎顔面インプラント学会, Nov. 2017, Japanese Journal of Maxillo Facial Implants, 16(3) (3), 233 - 233, Japanese


• CBCTを用いた実物大臓器立体モデル作製の基礎検討

  西尾卓郎, 西尾卓郎, 山本慶彦, 福富朗世, 宮本昌宜, 香川清澄, 根宜典行, 日下亜起子, 高橋哲, 明石昌也, 筧康正

  (公社)日本診療放射線技師会, Sep. 2017, 日本診療放射線技師会誌, 64(9) (9), 1220 - 1220, Japanese


• 重症心身障害者における動揺歯チェック表を用いた動揺歯の脱落予防に対する取り組み

  後藤 佳代子, 柴田 里紗, 明石 昌也, 玉村 宣尚

  日本重症心身障害学会, Aug. 2017, 日本重症心身障害学会誌, 42(2) (2), 257 - 257, Japanese


• 顎口腔領域の重症感染症例の検討

  高橋 淳子, 古土井 春吾, 岸本 恵実, 岩田 英治, 楠元 順哉, 梶 真人, 後藤 育子, 明石 昌也, 古森 孝英

  5年間に入院加療を要した顎口腔領域の重症感染症例40例(平均61.1歳、範囲7〜90歳、男性14例)について臨床的に検討した。臨床診断名は蜂窩織炎33例、下顎骨骨髄炎5例、壊死性筋膜炎2例であり、原因疾患は根尖性歯周炎17例、辺縁性歯周炎5例、智歯周囲炎5例などで原因部位は下顎大臼歯部が多かった。起因菌として通性嫌気性菌ではStreptococcus属、偏性嫌気性菌ではPeptostreptococcus属、Prevotella属が多く、抗菌薬投与日数の中央値は5.5日であった。また、下顎臼歯部を原因とする症例は難治性になりやすく、易感染性の基礎疾患を有する症例は抗菌薬投与日数が長くなる傾向にあり、発症から当科受診までの期間が長くなるほど、CRP値が高いほど治療期間が延長する傾向がみられた。早期の消炎手術は治療期間(抗菌薬投与期間)の短縮につながると考えられた。

  (一社)日本口腔感染症学会, Jul. 2017, 日本口腔感染症学会雑誌, 24(1) (1), 14 - 20, Japanese


• スニチニブに関連して発症したと考えられる下顎骨壊死の1例

  岩田 英治, 古土井 春吾, 岸本 恵実, 藤林 淳子, 楠元 順哉, 後藤 育子, 明石 昌也, 古森 孝英

  (一社)日本口腔感染症学会, Jul. 2017, 日本口腔感染症学会雑誌, 24(1) (1), 51 - 51, Japanese


• 口腔癌術後に消化管穿孔を発症した1例

  高橋 淳子, 古土井 春吾, 岸本 恵実, 岩田 英治, 楠元 順哉, 梶 真人, 後藤 育子, 明石 昌也, 古森 孝英

  (一社)日本口腔感染症学会, Jul. 2017, 日本口腔感染症学会雑誌, 24(1) (1), 54 - 55, Japanese


• 結節性硬化症患者に対する口腔衛生管理の現状 エベロリムスによる口内炎への対応

  西井 美佳, 古土井 春吾, 岸本 恵実, 岩田 英治, 楠元 順哉, 明石 昌也, 古森 孝英

  (一社)日本口腔感染症学会, Jul. 2017, 日本口腔感染症学会雑誌, 24(1) (1), 61 - 61, Japanese


• 当院における顎骨骨髄炎外来の現状と役割

  岩田 英治, 古土井 春吾, 鰐渕 聡, 明石 昌也, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2017, 日本口腔科学会雑誌, 66(2) (2), 192 - 192, Japanese


• 当科における予防的頸部郭清術に関する後ろ向き検討

  松田 彩, 筧 康正, 正井 友里子, 米澤 奈津季, 榊原 晶子, 長谷川 巧実, 明石 昌也, 南川 勉, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2017, 日本口腔科学会雑誌, 66(2) (2), 113 - 113, Japanese


• 口腔扁平上皮癌における術後早期(1年以内)の予後不良例に関する臨床的検討

  正井 友里子, 南川 勉, 松田 彩, 米澤 奈津季, 筧 康正, 榊原 晶子, 長谷川 巧実, 明石 昌也, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2017, 日本口腔科学会雑誌, 66(2) (2), 114 - 114, Japanese


• 4DCTによる咀嚼時の下顎頭前方滑走の解析

  明石昌也, 大槻有美, 黄文蘇, 筧康正, 古森孝英

  (一社)日本顎関節学会, Jul. 2017, 日本顎関節学会雑誌, 29(Suppl.) (Suppl.), 92 - 92, Japanese


• 口腔癌手術における嚥下改善手術に関する臨床的検討

  筧 康正, 南川 勉, 正井 友里子, 松田 彩, 米澤 奈津季, 榊原 晶子, 長谷川 巧実, 明石 昌也, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2017, 日本口腔科学会雑誌, 66(2) (2), 114 - 114, Japanese


• 両腋窩動脈狭窄症によりスティール症候群をきたし手指切断に至った透析患者の一例

  豊田 大祐, 加藤 敏輝, 芝田 圭佑, 齋藤 謙司, 花田 昌也, 明石 晋太郎

  (一社)日本透析医学会, May 2017, 日本透析医学会雑誌, 50(Suppl.1) (Suppl.1), 756 - 756, Japanese


• CAT分類における下顎骨切除範囲別の咀嚼機能と口腔QOLの評価

  前田 道徳, 渋谷 恭之, 明石 昌也, 古森 孝英

  (一社)日本頭頸部癌学会, May 2017, 頭頸部癌, 43(2) (2), 170 - 170, Japanese


• 神経症状を伴った下顎放射線性顎骨壊死における下歯槽神経の病理組織学的研究

  明石 昌也, 楠元 順哉, 長谷川 巧実, 南川 勉, 橋川 和信, 古森 孝英

  (一社)日本頭頸部癌学会, May 2017, 頭頸部癌, 43(2) (2), 189 - 189, Japanese


• 頭頸部癌化学放射線治療患者における口腔内細菌数と口腔粘膜水分量の解析

  西井美佳, 筧康正, 難波渚, 澤田麻衣子, 岩田英治, 長谷川巧実, 明石昌也, 古土井春吾, 古森孝英

  (一社)日本口腔ケア学会, Apr. 2017, 日本口腔ケア学会雑誌, 11(3) (3), 121 - 121, Japanese


• チタンミニプレートと固定用スクリューの関与が疑われた乾癬様皮疹の1例

  可信 雅彦, 明石 昌也, 古土井 春吾, 古森 孝英

  (NPO)日本口腔科学会, Mar. 2017, 日本口腔科学会雑誌, 66(1) (1), 54 - 54, Japanese


• 口腔癌放射線治療における新規併用療法の開発

  岩田英治, 長谷川巧実, 武田大介, 斎藤泉, 有本智美, 明石昌也, 南川勉, 古森孝英

  2017, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 35th


• 神戸大学医学部附属病院における心臓血管外科患者に対する周術期口腔機能管理の現状

  藤 大補, 古土井 春吾, 明石 昌也, 西井 美佳, 古森 孝英

  2013年8月〜11月迄の4ヵ月間に、周術期口腔機能管理を依頼され、歯科口腔外科を受診した例を対象に、心臓血管外科患者に対する、周術期口腔機能管理の現状について調査した。対象期間中に循環器内科、心臓血管外科より口腔機能管理の依頼があったのは125例で、このうち心臓血管外科手術が行われた109例(男性57例、女性52例、27〜92歳、平均62.1歳)を対象とした。原疾患の内訳は弁膜疾患52例、動脈瘤32例、冠動脈疾患18例、動脈解離15例、その他24例で弁膜疾患が最も多くを占めた。JSC2008に準じ、抗菌薬の感染性心内膜炎(IE)の予防のため抗菌薬投与が行われた患者の割合はクラスI 4例、IIa 50例、IIb 4例で合計58例、IE発症リスクが低いため抗菌薬を予防投与しなかった症例は51例であった。転院などで詳細不明な10例を除いた99例中、退院時に抗凝固薬や抗血小板薬が投与された症例は68例、施行されていない症例は31例であった。

  (一社)日本口腔感染症学会, Jan. 2017, 日本口腔感染症学会雑誌, 23(2) (2), 43 - 47, Japanese


• 当科における予防的頸部郭清術に関する後ろ向き検討

  松田彩, 筧康正, 正井友里子, 米澤奈津季, 榊原晶子, 長谷川巧実, 明石昌也, 南川勉, 古森孝英

  2017, 日本口腔科学会学術集会プログラム・抄録集, 71st, 181, Japanese


• 口腔扁平上皮癌における術後早期(1年以内)の予後不良例に関する臨床的検討

  正井友里子, 南川勉, 松田彩, 米澤奈津季, 筧康正, 榊原晶子, 長谷川巧実, 明石昌也, 古森孝英

  2017, 日本口腔科学会学術集会プログラム・抄録集, 71st, 183, Japanese


• 口腔癌手術における嚥下改善手術に関する臨床的検討

  筧康正, 南川勉, 正井友里子, 松田彩, 米澤奈津季, 榊原晶子, 長谷川巧実, 明石昌也, 古森孝英

  2017, 日本口腔科学会学術集会プログラム・抄録集, 71st, 183, Japanese


• Risk factors associated with oroantral perforation during surgical removal of maxillary third molar teeth.

  Takumi Hasegawa, Akira Tachibana, Daisuke Takeda, Eiji Iwata, Satomi Arimoto, Akiko Sakakibara, Masaya Akashi, Takahide Komori

  PURPOSE: The relationship between radiographic findings and the occurrence of oroantral perforation is controversial. Few studies have quantitatively analyzed the risk factors contributing to oroantral perforation, and no study has reported multivariate analysis of the relationship(s) between these various factors. This retrospective study aims to fill this void. METHODS: Various risk factors for oroantral perforation during maxillary third molar extraction were investigated by univariate and multivariate analysis. The proximity of the roots to the maxillary sinus floor (root-sinus [RS] classification) was assessed using panoramic radiography and classified as types 1-5. The relationship between the maxillary second and third molars was classified according to a modified version of the Archer classification. The relative depth of the maxillary third molar in the bone was classified as class A-C, and its angulation relative to the long axis of the second molar was also recorded. RESULTS: Performance of an incision (OR 5.16), mesioangular tooth angulation (OR 6.05), and type 3 RS classification (i.e., significant superimposition of the roots of all posterior maxillary teeth with the sinus floor; OR 10.18) were all identified as risk factors with significant association to an outcome of oroantral perforation. CONCLUSION: To our knowledge, this is the first multivariate analysis of the risk factors for oroantral perforation during surgical extraction of the maxillary third molar. This RS classification may offer a new predictive parameter for estimating the risk of oroantral perforation.

  Dec. 2016, Oral and maxillofacial surgery, 20(4) (4), 369 - 375, English, International magazine


• 当科におけるインプラント埋入症例と喪失リスクに関する臨床的検討

  片岡 侑嗣, 長谷川 巧実, 綿越 健太, 浅井 雅敏, 有本 智美, 明石 昌也, 鈴木 泰明, 古森 孝英

  (公社)日本顎顔面インプラント学会, Nov. 2016, Japanese Journal of Maxillo Facial Implants, 15(3) (3), 218 - 218, Japanese


• 内視鏡支援下で上顎洞迷入インプラントの摘出後に骨造成術を行った1例

  山下淳也, 山下淳也, 鈴木泰明, 筧康正, 木本明, 長谷川巧実, 明石昌也, 古森孝英

  (公社)日本顎顔面インプラント学会, Nov. 2016, Japanese Journal of Maxillo Facial Implants, 15(3) (3), 207 - 207, Japanese


• 放射線性顎骨壊死症例に対する画像診断を中心とした臨床的検討

  鰐渕 聡, 明石 昌也, 岩田 英治, 楠元 順哉, 松本 耕祐, 南川 勉, 古土井 春吾, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2016, 日本口腔科学会雑誌, 65(2) (2), 130 - 131, Japanese


• 当科における埋伏智歯抜歯後神経系合併症に対する治療法の現状

  平岡 佑二郎, 明石 昌也, 松本 耕祐, 榊原 晶子, 木本 明, 長谷川 巧実, 鈴木 泰明, 南川 勉, 古土井 春吾, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2016, 日本口腔科学会雑誌, 65(2) (2), 176 - 177, Japanese


• 当科における歯性上顎洞炎の臨床的検討

  岩田 英治, 明石 昌也, 岸本 恵実, 藤林 淳子, 楠元 順哉, 古土井 春吾, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2016, 日本口腔科学会雑誌, 65(2) (2), 220 - 221, Japanese


• 化学放射線療法による口腔粘膜炎の重症度を予測する因子についての検討

  岸本 恵実, 古土井 春吾, 藤林 淳子, 岩田 英治, 楠元 順哉, 梶 真人, 後藤 育子, 明石 昌也, 古森 孝英

  (一社)日本口腔感染症学会, Jul. 2016, 日本口腔感染症学会雑誌, 23(1) (1), 21 - 22, Japanese


• 歯性感染症が原因となった脳膿瘍の2例

  藤林 淳子, 古土井 春吾, 岸本 恵実, 岩田 英治, 楠元 順哉, 梶 真人, 後藤 育子, 明石 昌也, 古森 孝英

  (一社)日本口腔感染症学会, Jul. 2016, 日本口腔感染症学会雑誌, 23(1) (1), 25 - 26, Japanese


• 脳神経外科術後の顎関節症の臨床的検討

  大槻 有美, 明石 昌也, 黄 文蘇, 古森 孝英

  (一社)日本顎関節学会, Jul. 2016, 日本顎関節学会雑誌, 28(Suppl.) (Suppl.), 111 - 111, Japanese


• 顎関節に生じた滑膜骨軟骨腫症の1例

  黄 文蘇, 明石 昌也, 大槻 有美, 橘 進彰, 古森 孝英

  (一社)日本顎関節学会, Jul. 2016, 日本顎関節学会雑誌, 28(Suppl.) (Suppl.), 153 - 153, Japanese


• 下顎骨骨折の治療を行ったLennox-Gastaut症候群患児の1例

  中西 洋介, 松本 耕祐, 小守 紗也華, 長谷川 巧実, 明石 昌也, 柚鳥 宏和, 橘 進彰, 古森 孝英

  (一社)日本外傷歯学会, Jul. 2016, 日本外傷歯学会総会・学術大会プログラム・抄録集, 16回, 41 - 41, Japanese


• 当科におけるFibro-osseous lesionsの臨床的検討

  松尾 健司, 明石 昌也, 筧 康正, 松本 耕祐, 長谷川 巧実, 南川 勉, 古土井 春吾, 橘 進彰, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2016, 日本口腔科学会雑誌, 65(2) (2), 127 - 128, Japanese


• 遺伝子解析により確定診断を得た頬粘膜MALTリンパ腫の1例

  筧 康正, 明石 昌也, 木本 明, 長谷川 巧実, 南川 勉, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2016, 日本口腔科学会雑誌, 65(2) (2), 214 - 214, Japanese


• 当院における過去8年間の外傷歯患者の臨床統計的検討~顎骨骨折の有無と外傷歯の関係について~

  可信雅彦, 筧康正, 明石昌也, 古森孝英

  (一社)日本外傷歯学会, Jul. 2016, 日本外傷歯学会総会・学術大会プログラム・抄録集, 16回, 29 - 29, Japanese


• 全身麻酔挿管中に生じる歯牙損傷の後ろ向き調査

  小守紗也華, 筧康正, 明石昌也, 古森孝英

  (一社)日本外傷歯学会, Jul. 2016, 日本外傷歯学会総会・学術大会プログラム・抄録集, 16回, 30 - 30, Japanese


• 当科における顎関節症脱臼の臨床的検討

  大槻有美, 明石昌也, 黄文蘇, 筧康正, 古森孝英

  (一社)日本顎関節学会, Jul. 2016, 日本顎関節学会雑誌, 28(Suppl.) (Suppl.), 130 - 130, Japanese


• 4D-CTによる下顎再建術後患者の咀嚼の観察

  明石 昌也, 橋川 和信, 渋谷 恭之, 寺師 浩人, 古森 孝英

  (一社)日本頭頸部癌学会, May 2016, 頭頸部癌, 42(2) (2), 147 - 147, Japanese


• 歯科インプラント埋入のためのデンタルCTを用いた移植腓骨の形態評価

  明石 昌也, 橋川 和信, 鰐渕 聡, 楠元 順哉, 長谷川 巧実, 南川 勉, 寺師 浩人, 古森 孝英

  (一社)日本頭頸部癌学会, May 2016, 頭頸部癌, 42(2) (2), 147 - 147, Japanese


• 下顎枝垂直骨切り術後の骨癒合に対する低出力超音波パルス(LIPUS)の効果

  有本 智美, 長谷川 巧実, 岡本 奈々, 塩屋園 敦, 立石 千鶴, 明石 昌也, 鈴木 泰明, 古土井 春吾, 古森 孝英

  (NPO)日本顎変形症学会, May 2016, 日本顎変形症学会雑誌, 26(2) (2), 183 - 183, Japanese


• 当科における心臓血管外科周術期の口腔機能管理に関する実態と今後の課題

  難波 渚, 西井 美佳, 田村 恵利, 富田 和, 長谷川 巧実, 明石 昌也, 古土井 春吾, 古森 孝英

  (一社)日本口腔ケア学会, Mar. 2016, 日本口腔ケア学会雑誌, 10(1) (1), 199 - 199, Japanese


• 当院における周術期口腔機能管理に関する実態調査と今後の課題

  田村 惠利, 西井 美佳, 冨田 和, 難波 渚, 長谷川 巧実, 明石 昌也, 古土井 春吾, 古森 孝英

  (一社)日本口腔ケア学会, Mar. 2016, 日本口腔ケア学会雑誌, 10(1) (1), 225 - 225, Japanese


• 再建プレート露出に対する二期再建術前PET/CTが膿瘍の検知に有用であった1例

  松尾 健司, 明石 昌也, 楠元 順也, 藤林 淳子, 渡邊 一博, 南川 勉, 橋川 和信, 古森 孝英

  (NPO)日本口腔科学会, Mar. 2016, 日本口腔科学会雑誌, 65(1) (1), 69 - 69, Japanese


• 口腔扁平上皮癌における外的刺激受容体(TRPVチャネル)の発現亢進

  榊原晶子, 榊原俊介, 楠元順哉, 楠元順哉, 武田大介, 長谷川巧実, 明石昌也, 南川勉, 橋川和信, 古森孝英

  2016, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 34th


• 当科におけるFibro‐osseous lesionsの臨床的検討

  松尾健司, 明石昌也, 筧康正, 松本耕祐, 長谷川巧実, 南川勉, 古土井春吾, 橘進彰, 古森孝英

  2016, 日本口腔科学会学術集会プログラム・抄録集, 70th, 159, Japanese


• 術前18F‐FDG PET/CT撮影での口腔癌原発巣における定量評価の有用性

  米澤奈津季, 南川勉, 北島一宏, 高橋佑輔, 筧康正, 榊原晶子, 長谷川巧実, 明石昌也, 古森孝英

  2016, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 34th, 231, Japanese


• 口腔扁平上皮癌被膜外浸潤症例の臨床病理学的検討

  筧康正, 南川勉, 寺岡駿, 米澤奈津季, 高橋佑輔, 長谷川巧実, 明石昌也, 古森孝英

  2016, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 34th, 234, Japanese


• 口腔扁平上皮癌患者の遠位レベル(Level4,5)への頸部リンパ節転移が予後に与える影響

  定兼啓倫, 長谷川巧実, 渋谷恭之, 渋谷恭之, 米澤奈津季, 武田大介, 高橋佑輔, 筧康正, 明石昌也, 南川勉, 古森孝英

  2016, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 34th, 221, Japanese


• 局所的炭酸ガス投与による皮弁壊死抑制効果

  齊藤泉, 長谷川巧実, 武田大介, 岩田英治, 有本智美, 筧康正, 高橋佑輔, 榊原晶子, 明石昌也, 南川勉, 古森孝英

  2016, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 34th, 208, Japanese


• 遺伝子解析により確定診断を得た頬粘膜MALTリンパ腫の1例

  筧康正, 明石昌也, 木本明, 長谷川巧実, 南川勉, 古森孝英

  2016, 日本口腔科学会学術集会プログラム・抄録集, 70th, 286, Japanese


• 移植腓骨と下顎骨の癒合における骨膜温存の重要性

  明石昌也, 筧康正, 榊原晶子, 長谷川巧実, 南川勉, 橋川和信, 古森孝英

  2016, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 34th, 208, Japanese


• Targeted Disruption of JCAD/KIAA1462, a Coronary Artery Disease-associated Gene Product, Inhibits Angiogenic Processes in Vitro and in Vivo

  Tetsuya Hara, Tomoko Monguchi, Masaya Akashi, Noriko Iwamoto, Kenta Mori, Ryuji Toh, Yasuhiro Irino, Masakazu Shinohara, Mikio Furuse, Tatsuro Ishida, Ken-ichi Hirata

  LIPPINCOTT WILLIAMS & WILKINS, Nov. 2015, CIRCULATION, 132, English

  Summary international conference


• 入院加療を要した口腔頸部の重症感染症例の検討

  藤林 淳子, 古土井 春吾, 畑 みどり, 楠元 順哉, 後藤 育子, 明石 昌也, 古森 孝英

  (一社)日本口腔感染症学会, May 2015, 日本口腔感染症学会雑誌, 22(1) (1), 35 - 36, Japanese


• 口腔扁平上皮癌臨床検体におけるミトコンドリア量について

  武田大介, 長谷川巧実, 今井佑輔, 榊原晶子, 明石昌也, 南川勉, 古森孝英

  2015, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 33rd


• Clinical investigation of vascular malformations of the oral region treated by multiple photocoagulation with an Nd:YAG laser

  Matsumoto Kousuke, Ono Masataka, Kitayama Midori, Akashi Masaya, Suzuki Hiroaki, Komori Takahide

  Japanese Stomatological Society, 2015, Oral science in Japan, 63 - 65, English


• Clinical analysis of the chronological change in bone mass following sinus floor elevation

  Suzuki Hiroaki, Matsumoto Kousuke, Enomoto Yui, Kakei Yasumasa, Kimoto Akira, Akashi Masaya, Komori Takahide

  Japanese Stomatological Society, 2015, Oral science in Japan, 79 - 82, English


• 当科における頸部郭清術術後の頸部ドレーンに関する臨床的検討

  齊藤泉, 長谷川巧実, 米澤奈津季, 武田大介, 高橋佑輔, 筧康正, 松井太輝, 榊原晶子, 明石昌也, 南川勉, 古森孝英

  2015, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 33rd, 295, Japanese


• 当科における放射線性顎骨壊死に関する臨床的検討

  鰐渕聡, 明石昌也, 筧康正, 榊原晶子, 長谷川巧実, 南川勉, 古森孝英

  2015, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 33rd, 237, Japanese


• 当科における口腔扁平上皮癌遠隔転移例の臨床的検討とリスク因子について

  棚倉万紀子, 長谷川巧実, 米澤奈津季, 武田大介, 高橋佑輔, 筧康正, 松井太輝, 榊原晶子, 明石昌也, 南川勉, 古森孝英

  2015, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 33rd, 301, Japanese


• 口腔癌原発巣におけるDual‐time‐point FDG PETの有用性に関する検討

  米澤奈津季, 南川勉, 高橋佑輔, 筧康正, 松井太輝, 榊原晶子, 長谷川巧実, 明石昌也, 古森孝英

  2015, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 33rd, 188, Japanese


• 【全身疾患と抜歯-最新の知識による適切な対応-】 全身疾患を有する患者の抜歯の際に注意すべき基本的事項

  Komori Takahide, Akashi Masaya, Suzuki Yasuaki, 榊原晶子, Kimoto Akira, Minamikawa Tsutomu, Hasegawa Takumi, Furudoi Shungo

  (株)第一歯科出版, Jan. 2015, 歯科医療, 29(1) (1), 4 - 10, Japanese

  [Invited]

  Introduction scientific journal


• 【全身疾患と抜歯-最新の知識による適切な対応-】 循環器系疾患を有する患者の抜歯

  Akashi Masaya, Komori Takahide

  (株)第一歯科出版, Jan. 2015, 歯科医療, 29(1) (1), 11 - 18, Japanese

  [Invited]

  Introduction commerce magazine


• 遊離腓骨皮弁移植におけるMR Angiographyと超音波検査による下肢血管解剖の術前評価

  楠元 順哉, 橋川 和信, 寺師 浩人, 明石 昌也, 榊原 晶子

  (一社)日本形成外科学会, Nov. 2014, 日本形成外科学会会誌, 34(11) (11), 852 - 853, Japanese


• 当科における広範囲顎骨支持型インプラント補綴の臨床統計的検討

  今井 佑輔, 長谷川 巧実, 川畑 真哉, 武田 大介, 明石 昌也, 鈴木 泰明, 古森 孝英

  (公社)日本顎顔面インプラント学会, Nov. 2014, Japanese Journal of Maxillo Facial Implants, 13(3) (3), 128 - 128, Japanese


• 著明な上顎洞粘膜肥厚を有する患者に洞底挙上術後インプラント治療を行った1例

  榎本 由依, 鈴木 泰明, 木本 明, 長谷川 巧実, 明石 昌也, 浅井 知子, 竹内 純一郎, 古森 孝英

  (公社)日本顎顔面インプラント学会, Nov. 2014, Japanese Journal of Maxillo Facial Implants, 13(3) (3), 144 - 144, Japanese


• 当科におけるインプラント242例907本埋入に関する臨床的検討

  川畑 真哉, 長谷川 巧実, 今井 佑輔, 木本 明, 明石 昌也, 鈴木 泰明, 古森 孝英

  (公社)日本顎顔面インプラント学会, Nov. 2014, Japanese Journal of Maxillo Facial Implants, 13(3) (3), 160 - 160, Japanese


• 歯科インプラント埋入のためのCT画像を用いた移植腓骨の形態評価

  明石 昌也, 渋谷 恭之, 鰐渕 聡, 木本 明, 長谷川 巧実, 鈴木 泰明, 古森 孝英

  (公社)日本顎顔面インプラント学会, Nov. 2014, Japanese Journal of Maxillo Facial Implants, 13(3) (3), 166 - 166, Japanese


• 再発胃癌切除後に生じた胸腹壁欠損に対する治療経験

  長谷川 泰子, Nomura Tadashi, 橋川 和信, 寺師 浩人, 明石 昌也, 前田 翔

  (一社)日本形成外科学会, Nov. 2014, 日本形成外科学会会誌, 34(11) (11), 851 - 851, Japanese

  Others


• Preformed Reconstruction plateで下顎再建を行った2症例

  竹内 惇平, 明石 昌也, 藤 大補, 高橋 佑輔, 古土井 春吾, 榊原 晶子, 南川 勉, 渋谷 恭之, 古森 孝英

  (NPO)日本口腔科学会, Sep. 2014, 日本口腔科学会雑誌, 63(4) (4), 350 - 350, Japanese


• 放射線性口腔粘膜炎の重症度判定の客観性を高めるための方策

  川下 由美子, 林田 咲, 船原 まどか, 齋藤 俊行, 西井 美佳, 後藤 郁子, 明石 昌也, 古土井 春吾, 古森 孝英, 梅田 正博

  (NPO)日本口腔科学会, Sep. 2014, 日本口腔科学会雑誌, 63(4) (4), 358 - 358, Japanese


• 造血器悪性腫瘍患者への歯科的介入に関する前向き検討

  辻 和志, 渋谷 恭之, 岸本 恵実, 明石 昌也, 古土井 春吾, 古森 孝英

  (NPO)日本口腔科学会, Sep. 2014, 日本口腔科学会雑誌, 63(4) (4), 376 - 376, Japanese


• 口腔癌再建術後の頸部感染評価目的でのCTの有用性

  明石 昌也, 古土井 春吾, 南川 勉, 木本 明, 榊原 晶子, 長谷川 巧実, 後藤 育子, 渋谷 恭之, 古森 孝英

  (NPO)日本口腔科学会, Sep. 2014, 日本口腔科学会雑誌, 63(4) (4), 381 - 381, Japanese


• ビスフォスフォネート関連顎骨壊死症例の転帰に影響する因子についての検討

  古土井 春吾, 楠元 順哉, 畑 みどり, 藤林 淳子, 梶 真人, 後藤 育子, 明石 昌也, 吉位 尚, 古森 孝英

  2006年2月〜2012年3月に歯科口腔外科を受診し6ヵ月以上経過観察したビスホスホネート関連顎骨壊死(BRONJ)症例33例(男性7例、女性26例)を対象に分析し、転帰に影響する因子について検討した。治療法については原因歯の抜歯、抜歯窩掻爬、腐骨分離前の腐骨除去、顎骨切除など外科的処置を行った症例を外科的処置群、保存的処置を行った症例を保存的処置群とした。転帰評価方法は2013年2月時点、症状が消失した症状消失、ステージが改善した症状改善、変わらない症状不変、悪化した症状悪化に分けた。患者背景は性別は女性に多く、年齢は平均70.3(53〜85)歳、部位別では上顎9例(27.3%)、下顎22例(66.7%)、上下顎2例(2%)で、下顎に発症した症例が多く、投与経路はBP経口剤が多かった。初診時のステージ分類は2010年のBRONJにおける分類でステージ0が9例、ステージ1が9例(各27.3%)、ステージ2が10例(30.3%)、ステージ3が5例(15.1%)であった。発症の誘因として抜歯による症例が多く占めていた。転帰については症状消失24例(72.7%)、症状改善1例(3%)、症状不変8例(24.2%)であった。転帰に影響する因子はBP注射剤による症例、BP製剤を休薬できなかった症例、全身的危険因子を有する難治例が多い傾向がみられた。

  (一社)日本口腔感染症学会, Jun. 2014, 日本口腔感染症学会雑誌, 21(1) (1), 2 - 7, Japanese


• 当科における重症歯性感染症例の臨床的検討

  藤林 淳子, 古土井 春吾, 小松原 秀紀, 楠元 順哉, 後藤 育子, 明石 昌也, 古森 孝英

  (一社)日本口腔感染症学会, Jun. 2014, 日本口腔感染症学会雑誌, 21(1) (1), 49 - 49, Japanese


• 過去15年間の高齢口腔癌患者に関する臨床的検討

  米澤 奈津季, 南川 勉, 高橋 佑輔, 明石 昌也, 渋谷 恭之, 古森 孝英

  (一社)日本頭頸部癌学会, May 2014, 頭頸部癌, 40(2) (2), 158 - 158, Japanese


• 口腔癌再建術後の感染性合併症 術後CRPのabnormal responseに注目して

  明石 昌也, 橋川 和信, 南川 勉, 渋谷 恭之, 古森 孝英

  (一社)日本頭頸部癌学会, May 2014, 頭頸部癌, 40(2) (2), 182 - 182, Japanese


• 放射線誘発癌が疑われた口腔癌に対する遊離皮弁再建

  明石 昌也, 橋川 和信, 寺師 浩人

  (一社)日本形成外科学会, Mar. 2014, 日本形成外科学会会誌, 34(3) (3), 238 - 238, Japanese


• 口腔外に肉芽形成をきたしたビスフォスフォネート関連顎骨壊死の1例

  岩田 英治, 古土井 春吾, 楠元 順哉, 後藤 育子, 明石 昌也, 古森 孝英

  (NPO)日本口腔科学会, Mar. 2014, 日本口腔科学会雑誌, 63(2) (2), 246 - 246, Japanese


• 造血器悪性腫瘍患者への歯科的介入に関する前向き検討

  辻 和志, 渋谷 恭之, 明石 昌也, 古土井 春吾, 古森 孝英

  (NPO)日本口腔科学会, Jan. 2014, 日本口腔科学会雑誌, 63(1) (1), 65 - 66, Japanese


• 口腔癌cN1症例に対する頸部郭清術の郭清範囲についての検討

  筧康正, 南川勉, 高橋佑輔, 松井太輝, 長谷川巧実, 明石昌也, 重田崇至, 古土井春吾, 渋谷恭之, 古森孝英

  2014, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 32nd, 172, Japanese


• 舌癌180例のリンパ節転移に関する検討~健側転移について~

  松井太輝, 重田崇至, 南川勉, 明石昌也, 高橋佑輔, 筧康正, 古土井春吾, 渋谷恭之, 古森孝英

  2013, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 31st, 140, Japanese


• 口腔扁平上皮癌術後局所再発症例に関する検討

  高橋佑輔, 南川勉, 筧康正, 松井太輝, 榊原晶子, 明石昌也, 重田崇至, 渋谷恭之, 古森孝英

  2013, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 31st, 141, Japanese


• 口腔扁平上皮癌T4症例に対する手術療法の治療成績

  筧康正, 南川勉, 高橋佑輔, 松井太輝, 榊原晶子, 明石昌也, 重田崇至, 古土井春吾, 渋谷恭之, 古森孝英

  2013, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 31st, 144, Japanese


• プレート破折後に遊離腓骨皮弁で下顎骨再建を行った1例

  Akashi Masaya, Hashikawa Kazunobu, Tahara Shinya

  (一社)日本形成外科学会, Sep. 2012, 日本形成外科学会会誌, 32(9) (9), 711 - 712, Japanese

  [Refereed]

  Meeting report


• 骨髄移植後GVHD患者に発生した口腔扁平上皮癌の2例

  筧康正, 南川勉, 松井太輝, 榊原晶子, 明石昌也, 三浦真香, 重田崇至, 小松原秀紀, 渋谷恭之, 古森孝英

  2012, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 30th, 211, Japanese


• 口腔扁平上皮癌に対する頸部郭清術施行205例の頸部転移様相

  松井 太輝, 重田 崇至, 高橋 英哲, 高橋 佑輔, 明石 昌也, 南川 勉, 渋谷 恭之, 梅田 正博, 古森 孝英

  (公社)日本口腔外科学会, Sep. 2011, 日本口腔外科学会雑誌, 57(Suppl.) (Suppl.), 99 - 99, Japanese


• 口腔に発生した紡錘細胞癌における肺転移巣の倍加時間について

  重田 崇至, 南川 勉, 小松原 秀紀, 榊原 晶子, 明石 昌也, 高橋 英哲, 松井 太輝, 渋谷 恭之, 梅田 正博, 古森 孝英

  (公社)日本口腔外科学会, Sep. 2011, 日本口腔外科学会雑誌, 57(Suppl.) (Suppl.), 191 - 191, Japanese


• 顎口腔再建におけるフェーズコントラスト型CT Angiographyの有用性について

  明石 昌也, 橋川 和信, 南川 勉, 重田 崇至, 榊原 晶子, 古土井 春吾, 渋谷 恭之, 古森 孝英

  (公社)日本口腔外科学会, Sep. 2011, 日本口腔外科学会雑誌, 57(Suppl.) (Suppl.), 215 - 215, Japanese


• 舌癌のstage I・II症例における臨床的検討について

  西村 裕貴, 重田 崇至, 高橋 佑輔, 松井 太輝, 高橋 英哲, 榊原 晶子, 明石 昌也, 南川 勉, 渋谷 恭之, 梅田 正博, 古森 孝英

  (公社)日本口腔外科学会, Sep. 2011, 日本口腔外科学会雑誌, 57(Suppl.) (Suppl.), 324 - 324, Japanese


• 当科における口底扁平上皮癌手術症例の臨床病理学的検討

  筧 康正, 南川 勉, 松井 太輝, 高橋 英哲, 榊原 晶子, 明石 昌也, 重田 崇至, 渋谷 恭之, 梅田 正博, 古森 孝英

  (公社)日本口腔外科学会, Sep. 2011, 日本口腔外科学会雑誌, 57(Suppl.) (Suppl.), 200 - 200, Japanese


• 口腔癌手術における嚥下改善手術に関する臨床的検討

  明石 昌也, 南川 勉, 高橋 英哲, 小國 晶子, 橋川 和信, 古土井 春吾, 渋谷 恭之, 梅田 正博, 古森 孝英

  (公社)日本口腔外科学会, Sep. 2010, 日本口腔外科学会雑誌, 56(Suppl.) (Suppl.), 140 - 140, Japanese


• 外側舌リンパ節転移を認めた口底癌の1例

  木本 明, 南川 勉, 小林 正樹, 服部 真季, 明石 昌也, 澁谷 恭之, 横尾 聡, 梅田 正博, 古森 孝英

  (公社)日本口腔外科学会, Jun. 2007, 日本口腔外科学会雑誌, 53(6) (6), 400 - 400, Japanese


• 顎関節症患者の臨床統計的検討

  渋谷 恭之, 竹内 純一郎, 池端 伯子, 藤田 剛史, 明石 昌也, 古森 孝英

  (一社)日本顎関節学会, Apr. 2006, 日本顎関節学会雑誌, 18(1) (1), 48 - 48, Japanese


• 当科における顎関節症III a患者の臨床統計的検討

  竹内 純一郎, 渋谷 恭之, 池端 伯子, 藤田 剛史, 明石 昌也, 古森 孝英

  (一社)日本顎関節学会, Apr. 2006, 日本顎関節学会雑誌, 18(1) (1), 48 - 48, Japanese


• 当科における顎関節症I・II患者の臨床統計的検討

  池端 伯子, 渋谷 恭之, 竹内 純一郎, 明石 昌也, 藤田 剛史, 古森 孝英

  (一社)日本顎関節学会, Apr. 2006, 日本顎関節学会雑誌, 18(1) (1), 80 - 80, Japanese


• 当科における顎関節症III b型患者の臨床統計的検討

  藤田 剛史, 渋谷 恭之, 竹内 純一郎, 池端 伯子, 明石 昌也, 古森 孝英

  (一社)日本顎関節学会, Apr. 2006, 日本顎関節学会雑誌, 18(1) (1), 80 - 81, Japanese


• 当科における顎関節症IV型患者の臨床統計的検討

  明石 昌也, 渋谷 恭之, 竹内 純一郎, 池端 伯子, 藤田 剛史, 古森 孝英

  (一社)日本顎関節学会, Apr. 2006, 日本顎関節学会雑誌, 18(1) (1), 81 - 81, Japanese

• 改訂版日常の口腔外科 病診連携１ＳＭＡＲＴ＆ＳＭＯＯＴＨ / V-7 顎関節疾患治療の概要を知る

  古森孝英, 横尾聡, 古土井春吾, 綿谷早苗, 梅田正博, 渋谷恭之, 藤岡学, 立石千鶴, 南川勉, 松本耕祐, 榊原晶子, 鈴木泰明, Akashi Masaya

  Others, 永末書店, 2018, Japanese

  Textbook


• Oral and Craniofacial Diseases and Disorders / Capter 2: Complications Following Radiation Therapy for Head and Neck Malignancy

  AkashiM, Komori Takahide, MinamikawaT, Hasegawa Takumi, NishiiM, HashikawaK, SeddikMH, SasakiR

  Others, Oxford University Press, 2018, English

  Scholarly book


• OPEN ACCESS e Books: Oral and Craniofacial Disease and Disorders. / Oral complications following radiation theraphy for head anad neck malignancy

  Akashi Masaya, KomoriT, MinamikawaT, HasegawaT, NishiiM, HashikawaH, SeddikMH, SasakiR

  Others, －, 2018, English

  Scholarly book


• 新こんな患者さんが歯科に来たときは？全身疾患・口腔外科疾患に対する診療マニュアル / 味覚障害への対応

  Akashi Masaya, 鄒天皓

  Others, 第一歯科出版, 2014, Japanese

  General book


• 新こんな患者さんが歯科に来たときは？全身疾患・口腔外科疾患に対する診療マニュアル / 腎障害患者への対応

  Akashi Masaya, 大槻有美, 若林玲奈, 高橋英哲

  Others, 第一歯科出版, 2014, Japanese

  General book


• 新こんな患者さんが歯科に来たときは？全身疾患・口腔外科疾患に対する診療マニュアル / 三叉神経痛への対応

  Akashi Masaya, 藤原健一, 高田直樹

  Others, 第一歯科出版, 2014, Japanese

  General book

• 心血管外科手術周術期の口腔機能管理に関する臨床的検討

  難波渚, Akashi Masaya, Komori Takahide, 桶本翔吾, 小槇 公大, 小川 真人, 坪井 康典, Kobayashi Seimi, Gotake Yasuko, Okita Yutaka

  第3回日本心臓リハビリテーション学会近畿地方会, Feb. 2018, Japanese, 日本心臓リハビリテーション学会, 神戸, Domestic conference

  Oral presentation


• 下顎骨壊死における下歯槽神経障害の臨床病理学的検討

  平岡佑二郎, AKASHI MASAYA, SHIGEOKA MANABU, 楠元順哉, HASEGAWA TAKUMI, KOMORI TAKAHIDE

  第62回日本口腔外科学会総会・学術大会, Oct. 2017, Japanese, 京都, Domestic conference

  Oral presentation


• FDG PET-CTは重症下顎放射線性顎骨壊死の切除範囲決定に有用か?．

  Akashi Masaya, Hashikawa Kazunobu, 楠元順哉, Furudoi Shungo, Komori Takahide

  第62回日本口腔外科学会総会・学術大会（京都）, Oct. 2017, Japanese, 日本口腔外科学会, 京都, Domestic conference

  Public symposium


• 神経症状を伴った下顎放射線性顎骨壊死における下歯槽神経の病理組織学的研究．

  Akashi Masaya, 楠元順哉, Hasegawa Takumi, Minamikawa Tsutomu, Hashikawa Kazunobu, Komori Takahide

  第41回日本頭頸部癌学会（京都）, Jun. 2017, Japanese, 日本頭頸部癌学会, 京都, Domestic conference

  Oral presentation


• 【シンポジウム2「頭頸部再建に残された課題－二次再建の挑戦と課題－」】重症下顎骨放射線骨髄炎に対する手術―デブリドマン範囲を病理組織学的検討結果から考察する―．

  Hashikawa Kazunobu, Akashi Masaya, 楠元順哉, Nomura Tadashi, Terashi Hiroto

  第60回日本形成外科学会総会・学術集会（大阪）, Apr. 2017, Japanese, 日本形成外科学会, 大阪, Domestic conference

  Public symposium


• 4DCT EVALUATION OF CONDYLAR MOVEMEN IN PATIENTS UNDERWENT MANDIBULAR RECONSTRUCTION．

  Akashi Masaya, Toshinori Sekitani, Yumi Ohtsuki, Yasumasa Kakei, Hasegawa Takumi, Noriyuki Negi, Hashikawa Kazunobu, Yasuyuki Shibuya, Takahashi Satoru, Komori Takahide

  23nd International Conference on Oral and Maxillofacial Surgery（Hong Kong）, Mar. 2017, Japanese, International Conference on Oral and Maxillofacial Surgery, Hong Kong, 海外, Domestic conference

  Oral presentation


• 口腔扁平上皮癌では外的刺激受容体（TRPVチャネル）の発現が亢進する．

  Sakakibara Akiko, 榊原俊介, 楠元順哉, 武田大介, Hasegawa Takumi, Akashi Masaya, Minamikawa Tsutomu, Hashikawa Kazunobu, Komori Takahide

  第35回口腔腫瘍学会（福岡）, Jan. 2017, Japanese, 口腔腫瘍学会, 福岡, Domestic conference

  Oral presentation


• 内視鏡支援下で上顎洞迷入インプラントの摘出後に骨造成術を行った1例

  山下淳也, Suzuki Yasuaki, 筧康正, 木本 明, Hasegawa Takumi, Akashi Masaya, Komori Takahide

  第20回日本顎顔面インプラント学会, Dec. 2016, Japanese, 日本顎顔面インプラント学会, 東京, Domestic conference

  Poster presentation


• 当科におけるインプラント埋入症例と喪失リスクに関する臨床的検討

  片岡侑嗣, Hasegawa Takumi, 綿越健太, 浅井雅敏, 有本智美, Akashi Masaya, Suzuki Yasuaki, Komori Takahide

  第20回日本顎顔面インプラント学会, Dec. 2016, Japanese, 日本顎顔面インプラント学会, 東京, Domestic conference

  Poster presentation


• チタンミニプレートと固定用スクリューの関与が疑われた乾癬様皮疹の1例

  可信雅彦, Akashi Masaya, Furudoi Shungo, Komori Takahide

  第28回口腔科学会近畿地方部会, Dec. 2016, Japanese, 日本口腔科学会, 大阪, Domestic conference

  Oral presentation


• 腓骨皮弁による下顎再建におけるミニプレートと再建用プレートの比較

  平岡佑二郎, Akashi Masaya, 筧 康正, 楠元順哉, Sakakibara Akiko, Hasegawa Takumi, Minamikawa Tsutomu, Hashikawa Kazunobu, Komori Takahide

  第61回日本口腔外科学会総会・学術大会, Nov. 2016, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Oral presentation


• 放射線性顎骨壊死の病理組織学的検討

  鰐渕 聡, Akashi Masaya, 岩田英治, 筧 康正, 楠元順哉, Minamikawa Tsutomu, Furudoi Shungo, Hashikawa Kazunobu, Komori Takahide

  第61回日本口腔外科学会総会・学術大会, Nov. 2016, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Oral presentation


• 頭頸部領域の蜂窩識炎に対する好中球/リンパ球比，血小板/リンパ球比の有用性の検討

  楠元順哉, Furudoi Shungo, 岸本惠実, 岩田英治, Akashi Masaya, Komori Takahide

  第61回日本口腔外科学会総会・学術大会, Nov. 2016, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Oral presentation


• 頭頸部蜂窩織炎に対する好中球/リンパ球比，血小板/リンパ球比の有用性の検討

  楠元 順哉, Furudoi Shungo, 岸本 恵実, 岩田 英治, 藤林 淳子, Akashi Masaya, Komori Takahide

  第61回公益社団法人日本口腔外科学会総会・学術大会, Nov. 2016, Japanese, 公益社団法人日本口腔外科学会総会・学術大会, 千葉市, Domestic conference

  Oral presentation


• 当科における舌癌・口底癌手術症例の再建方法に関する検討

  綿越健太, Minamikawa Tsutomu, 米澤奈津季, 筧 康正, Hasegawa Takumi, Sakakibara Akiko, Akashi Masaya, Furudoi Shungo, Komori Takahide

  第61回日本口腔外科学会総会・学術大会, Nov. 2016, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Poster presentation


• 当科における心臓血管外科開胸手術患者に対する周術期口腔機能管理の臨床的検討

  難波 渚, Akashi Masaya, 西井美佳, 楠元順哉, Matsumoto Kousuke, Hasegawa Takumi, Furudoi Shungo, Komori Takahide

  第61回日本口腔外科学会総会・学術大会, Nov. 2016, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Oral presentation


• 当科における口腔癌患者の口腔内細菌数の変化とSSIについて

  小守紗也華, Hasegawa Takumi, 西井美佳, Akashi Masaya, Furudoi Shungo, Komori Takahide

  第61回日本口腔外科学会総会・学術大会, Nov. 2016, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Oral presentation


• 造血器悪性腫瘍患者に対する化学療法中の歯性感染症が原因の敗血症発症率

  岸本惠実, Akashi Masaya, 辻 和志, 楠元順哉, Furudoi Shungo, Komori Takahide

  第61回日本口腔外科学会総会・学術大会, Nov. 2016, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Oral presentation


• 舌癌切除後の前腕皮弁再建における 切除検体サイズと皮弁サイズの 相関と機能の検討

  Sakakibara Akiko, 楠元 順哉, Hasegawa Takumi, Akashi Masaya, Minamikawa Tsutomu, Furudoi Shungo, Hashikawa Kazunobu, Komori Takahide

  第61回公益社団法人日本口腔外科学会総会・学術大会, Nov. 2016, Japanese, 公益社団法人日本口腔外科学会総会・学術大会, 千葉市, Domestic conference

  Oral presentation


• 舌癌切除後の前腕皮弁再建における切除検体サイズと皮弁サイズの僧冠と機能の検討

  Sakakibara Akiko, 楠元順哉, Hasegawa Takumi, Akashi Masaya, Minamikawa Tsutomu, Furudoi Shungo, Hashikawa Kazunobu, Komori Takahide

  第61回日本口腔外科学会総会・学術大会, Nov. 2016, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Oral presentation


• 口腔癌放射線治療における有効な新規併用療法の探索

  岩田英治, Hasegawa Takumi, 武田大介, 斉藤 泉, 有本智美, Akashi Masaya, Minamikawa Tsutomu, Komori Takahide

  第61回日本口腔外科学会総会・学術大会, Nov. 2016, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Poster presentation


• 口腔癌術後リンパ浮腫に関するCT解析

  寺岡 駿, Akashi Masaya, 筧 康正, 楠元順哉, Sakakibara Akiko, Hasegawa Takumi, Minamikawa Tsutomu, Hashikawa Kazunobu, Komori Takahide

  第61回日本口腔外科学会総会・学術大会, Nov. 2016, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Oral presentation


• 局所的炭酸ガス投与による皮弁壊死抑制効果

  齊藤 泉, Hasegawa Takumi, 武田大介, 岩田英治, 有本智美, Sakakibara Akiko, Akashi Masaya, Minamikawa Tsutomu, Komori Takahide

  第61回日本口腔外科学会総会・学術大会, Nov. 2016, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Poster presentation


• 下顎枝垂直骨切り術後の骨癒合に対する低出力超音波パルス(LIPUS)の効果

  有本智美, Hasegawa Takumi, 齊藤 泉, 岩田英治, 武田大介, 立石千鶴, Akashi Masaya, Suzuki Yasuaki, Furudoi Shungo, Komori Takahide

  第61回日本口腔外科学会総会・学術大会, Nov. 2016, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Poster presentation


• 遠位レベル(Level IV,V)への頸部リンパ節転移を生じた口腔扁平上皮癌患者の予後に関する後ろ向き観察研究

  Hasegawa Takumi, Minamikawa Tsutomu, 米澤奈津季, 高橋佑輔, 筧 康正, Sakakibara Akiko, Akashi Masaya, Komori Takahide

  第61回日本口腔外科学会総会・学術大会, Nov. 2016, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Oral presentation


• 口腔癌術後に消化管穿孔を発症した1例

  高橋淳子, Furudoi Shungo, 岸本恵美, 岩田英治, 楠元順哉, 梶 真人, 後藤育子, Akashi Masaya, Komori Takahide

  第25回日本口腔感染症学会総会・学術大会, Oct. 2016, Japanese, 日本口腔感染症学会, 神戸, Domestic conference

  Oral presentation


• 結節性硬化症患者に対する口腔衛生管理の現状ーエベロリムスによる口内炎への対応ー

  西井美佳, Furudoi Shungo, 岸本惠実, 岩田英治, 楠元順哉, Akashi Masaya, Komori Takahide

  第25回日本口腔感染症学会総会・学術大会, Oct. 2016, Japanese, 日本口腔感染症学会, 神戸, Domestic conference

  Oral presentation


• スニチニブに関連して発症したと考えられる下顎骨壊死の1例

  岩田英治, Furudoi Shungo, 岸本惠実, 高橋淳子, 楠元順哉, 後藤育子, Akashi Masaya, Komori Takahide

  第25回日本口腔感染症学会総会・学術大会, Oct. 2016, Japanese, 日本口腔感染症学会, 神戸, Domestic conference

  Oral presentation


• Development of the new treatment of OSCC by local application of transcutaneous carbon dioxide

  Iwata E, Hasegawa Takumi, Takeda D, Saito Izumi, Arimoto S, Akashi Masaya, Minamikawa Tsutomu, Komori Takahide

  The 23rd European Association for Cranio Maxillo-Facial Surgery (EACMFS), Sep. 2016, Japanese, UKレーザー歯学会, London, UK, International conference

  Poster presentation


• Determination of resection margins by using a combination of CT, MRI, PET, and 3D model in osteoradionecrosis of the jaw

  Akashi Masaya, Hashikawa Kazunobu, Satoshi Wanifuchi, Megumi Kishimoto, Eiji Iwata, Yasumasa Kakei, Junya Kusumoto, Matsumoto Kousuke, Sakakibara Akiko, Hasegawa Takumi, Minamikawa Tsutomu, Furudoi Shungo, Komori Takahide

  The 23th Congress of EACMFS, Sep. 2016, English, Congress of EACMFS, London, イギリス, International conference

  Oral presentation


• Determination of resection margins by using a combination of CT, MRI, PET, and 3D model in osteoradionecrosis of the jaw

  Akashi Masaya, Hashikawa Kazunobu, Wanifuchi S, Kishimoto M, Iwata E, Kakei Y, Kusumoto J, Matsumoto Kensuke, Sakakibara Akiko, Hasegawa Takumi, Minamikawa Tsutomu, Furudoi Shungo, Komori Takahide

  The 23rd European Association for Cranio Maxillo-Facial Surgery (EACMFS), Sep. 2016, Japanese, UKレーザー歯学会, London, UK, International conference

  Poster presentation


• Changes of cell junctions induced by EGFR inhibition in oral squamous cell carcinomas

  Kakei Y, Akashi Masaya, Teraoka S, Hasegawa Takumi, Komori Takahide

  The 23rd European Association for Cranio Maxillo-Facial Surgery (EACMFS), Sep. 2016, Japanese, UKレーザー歯学会, London, UK, International conference

  Poster presentation


• A clinical study of injured patients treated with dental implants

  Suzuki Yasuaki, Takeuchi J, Matsuo K, Kakei Y, Kimoto A, Hasegawa Takumi, Akashi Masaya, Chikazu D, Komori Takahide

  The 23rd European Association for Cranio Maxillo-Facial Surgery (EACMFS), Sep. 2016, Japanese, UKレーザー歯学会, London, UK, International conference

  Poster presentation


• A clinical study of 12 implants resulting in implant removal

  Takahashi D, Suzuki Yasuaki, Kakei Y, Hasegawa Takumi, Akashi Masaya, Minamikawa Tsutomu, Komori Takahide

  The 23rd European Association for Cranio Maxillo-Facial Surgery (EACMFS),, Sep. 2016, Japanese, UKレーザー歯学会, London, UK, International conference

  Poster presentation


• 頬部に認めた孤立性線維性腫瘍の1例

  鰐渕 聡, 大槻有美, 後藤育子, Akashi Masaya, Suzuki Yasuaki, Minamikawa Tsutomu, Furudoi Shungo, Komori Takahide

  第47回日本口腔外科学会近畿支部学術集会, Jul. 2016, Japanese, 日本口腔外科学会, 大阪, Domestic conference

  Oral presentation


• 脳神経外科術後の顎関節症の臨床的検討

  大槻有美, Akashi Masaya, 黄文蘇, 筧康正, Komori Takahide

  第29回日本顎関節学会総会・学術大会, Jul. 2016, Japanese, 日本顎関節学会, 箱根, Domestic conference

  Oral presentation


• 当科における心臓血管外科周術期の口腔機能管理に関する実態と今後の課題

  難波 渚, Akashi Masaya, 冨田 和, 田村恵利, 澤田麻衣子, 西井美佳, Hasegawa Takumi, Furudoi Shungo, Komori Takahide

  第32回兵庫県歯科医学大会, Jul. 2016, Japanese, 兵庫県歯科医師会, 神戸, Domestic conference

  Oral presentation


• 当科における顎関節脱臼の臨床的検討

  大槻有美, Akashi Masaya, 黄文蘇, 筧康正, Komori Takahide

  第29回日本顎関節学会総会・学術大会, Jul. 2016, Japanese, 日本顎関節学会, 箱根, Domestic conference

  Poster presentation


• 当院における過去8年間の外傷歯患者の臨床統計的検討 ～顎骨骨折の有無と外傷歯の関係について～

  可信雅彦, 筧 康正, 小守紗也華, Akashi Masaya, Furudoi Shungo, Komori Takahide

  第16回日本外傷歯学会総会・学術大会, Jul. 2016, Japanese, 日本外傷歯学会, 神戸, Domestic conference

  Oral presentation


• 造血器悪性腫瘍に対する化学療法中のD-indexと口腔内感染性合併症との関連についての検討

  Akashi Masaya, 岸本 恵実, 辻 和志, 楠元 順哉, Yakushijin Kimikazu, Kawamoto Shinichiro, 岡村 篤夫, Matsuoka Hiroshi, Furudoi Shungo, Komori Takahide

  第14回日本臨床腫瘍学会, Jul. 2016, Japanese, 日本臨床腫瘍学会, 神戸市, Domestic conference

  Oral presentation


• 全身麻酔挿管中に生じる歯の損傷も後ろ向き調査

  小守紗也華, 筧 康正, 可信雅彦, Akashi Masaya, Furudoi Shungo, Komori Takahide

  第16回日本外傷歯学会総会・学術大会, Jul. 2016, Japanese, 日本外傷歯学会, 神戸, Domestic conference

  Oral presentation


• 顎関節に生じた滑膜骨軟骨腫症の1例

  黄文蘇, Akashi Masaya, 大槻有美, 橘進彰, Komori Takahide

  第29回日本顎関節学会総会・学術大会, Jul. 2016, Japanese, 日本顎関節学会, 箱根, Domestic conference

  Poster presentation


• 下顎歯肉癌の精査においてCowden症候群の合併が示唆された1例

  榎本由依, Akashi Masaya, 平岡佑二郎, 船原隆一郎, Minamikawa Tsutomu, Komori Takahide

  第47回日本口腔外科学会近畿支部学術集会, Jul. 2016, Japanese, 日本口腔外科学会, 大阪, Domestic conference

  Oral presentation


• 下顎骨骨折の治療を行ったLennox-Gastaut症候群患児の1例

  中西洋介, Matsumoto Kousuke, 小守紗也華, Hasegawa Takumi, Akashi Masaya, 柚鳥宏和, 橘進彰, Komori Takahide

  第16回日本外傷歯学会総会・学術大会, Jul. 2016, Japanese, 日本外傷歯学会, 神戸, Domestic conference

  Oral presentation


• 15歳女児に生じた舌扁平上皮癌の1例

  綿越健太, Minamikawa Tsutomu, 寺岡 駿, Akashi Masaya, Komori Takahide

  第47回日本口腔外科学会近畿支部学術集会, Jul. 2016, Japanese, 日本口腔外科学会, 大阪, Domestic conference

  Oral presentation


• 歯科インプラント埋入のためのデンタルCTを用いた移植腓骨の形態評価

  Akashi Masaya, Hashikawa Kazunobu, 鰐渕 聡, 楠元順哉, Hasegawa Takumi, Minamikawa Tsutomu, Terashi Hiroto, Komori Takahide

  第40回日本頭頸部癌学会総会・学術大会, Jun. 2016, Japanese, 日本頭頸部癌学会, 埼玉, Domestic conference

  Oral presentation


• 歯科インプラント埋入のためのデンタルCTを用いた移植腓骨の形態評価

  Akashi Masaya, Hashikawa Kazunobu, 鰐渕聡, 楠元 順哉, Hasegawa Takumi, Minamikawa Tsutomu, Terashi Hiroto, Komori Takahide

  第40回日本頭頸部癌学会, Jun. 2016, Japanese, 日本頭頸部癌学会, さいたま市, Domestic conference

  Oral presentation


• 下顎枝垂直骨切り術後の骨癒合に対する低出力超音波パルス(LIPUS)の効果

  有本智美, Hasegawa Takumi, 岡本奈那, 塩屋園敦, 立石千鶴, Akashi Masaya, Suzuki Yasuaki, Furudoi Shungo, Komori Takahide

  第26回日本顎変形症学会総会・学術大会, Jun. 2016, Japanese, 日本顎変形症学会, 東京, Domestic conference

  Poster presentation


• 4D-CTによる下顎再建術後患者の咀嚼の観察

  Akashi Masaya, Hashikawa Kazunobu, 渋谷恭之, Terashi Hiroto, Komori Takahide

  第40回日本頭頸部癌学会総会・学術大会, Jun. 2016, Japanese, 日本頭頸部癌学会, 埼玉, Domestic conference

  Oral presentation


• 4D-CTによる下顎再建術後患者の咀嚼の観察

  Akashi Masaya, Hashikawa Kazunobu, 渋谷恭之, Terashi Hiroto, Komori Takahide

  第40回日本頭頸部癌学会, Jun. 2016, Japanese, 日本頭頸部癌学会, さいたま市, Domestic conference

  Oral presentation


• 放射線性顎骨鋭氏壊死症例に対する画像診断を中心とした臨床的検討

  鰐渕 聡, Akashi Masaya, 岩田英治, 楠元順哉, 松本耕佑, Minamikawa Tsutomu, Furudoi Shungo, Komori Takahide

  第70回日本口腔科学会総会・学術大会, Apr. 2016, Japanese, 日本口腔科学会, 福岡, Domestic conference

  Oral presentation


• 当科における埋伏智歯抜歯後神経系合併症に対する治療法の現状

  平岡佑二郎, Akashi Masaya, Matsumoto Kousuke, Sakakibara Akiko, 木本 明, Hasegawa Takumi, Suzuki Yasuaki, Minamikawa Tsutomu, Furudoi Shungo, Komori Takahide

  第70回日本口腔科学会総会・学術大会, Apr. 2016, Japanese, 日本口腔科学会, 福岡, Domestic conference

  Oral presentation


• 当科における歯性上顎洞炎の臨床的検討

  岩田英治, Akashi Masaya, 岸本恵実, 藤林淳子, 楠元順哉, Furudoi Shungo, Komori Takahide

  第70回日本口腔科学会総会・学術大会, Apr. 2016, Japanese, 日本口腔科学会, 福岡, Domestic conference

  Poster presentation


• 当科におけるFibro-osseous lesionsの臨床的検討

  松尾健司, Akashi Masaya, 筧 康正, Matsumoto Kousuke, Hasegawa Takumi, Minamikawa Tsutomu, Furudoi Shungo, 橘進彰, Komori Takahide

  第70回日本口腔科学会総会・学術大会, Apr. 2016, Japanese, 日本口腔科学会, 福岡, Domestic conference

  Oral presentation


• 中咽頭癌化学放射線治療患者における口腔衛生管理-強度変調放射線治療(IMRT)と従来型放射線治療(RT)の比較

  西井美佳, 筧 康正, 冨田 和, 難波 渚, 田村恵利, 澤田麻衣子, Akashi Masaya, Furudoi Shungo, Komori Takahide

  第13回日本口腔ケア学会総会・学術大会, Apr. 2016, Japanese, 日本口腔ケア学会, 千葉, Domestic conference

  Oral presentation


• 遺伝子解析により確定診断を得たMALTリンパ腫の1例

  筧 康正, Akashi Masaya, 木本 明, Hasegawa Takumi, Minamikawa Tsutomu, Komori Takahide

  第70回日本口腔科学会総会・学術大会, Apr. 2016, Japanese, 日本口腔科学会, 福岡, Domestic conference

  Oral presentation


• 腓骨皮弁採取部における一期縫縮と植皮の比較

  渡邊一博, Akashi Masaya, 楠本順哉, Matsumoto Kousuke, 木本 明, Sakakibara Akiko, Hasegawa Takumi, Minamikawa Tsutomu, Hashikawa Kazunobu, Komori Takahide

  第34回日本口腔腫瘍学会総会・学術大会, Jan. 2016, Japanese, 日本口腔腫瘍学会, 横浜, Domestic conference

  Poster presentation


• 術前18F-FDG PET/CT撮影での口腔癌原発巣における定量評価の有用性

  米澤奈津季, Minamikawa Tsutomu, 北島一宏, 高橋佑輔, 筧 康正, Sakakibara Akiko, Hasegawa Takumi, Akashi Masaya, Komori Takahide

  第34回日本口腔腫瘍学会総会・学術大会, Jan. 2016, Japanese, 日本口腔腫瘍学会, 横浜, Domestic conference

  Poster presentation


• 口腔扁平上皮癌被膜外浸潤症例の臨床病理学的検討

  筧 康正, Minamikawa Tsutomu, 寺岡 駿, 米澤奈津季, 高橋佑輔, Hasegawa Takumi, Akashi Masaya, Komori Takahide

  第34回日本口腔腫瘍学会総会・学術大会, Jan. 2016, Japanese, 日本口腔腫瘍学会, 横浜, Domestic conference

  Poster presentation


• 口腔扁平上皮癌患者の遠位レベル（level4,5）への頸部リンパ節転移が予後に与える影響

  定兼啓倫, Hasegawa Takumi, 渋谷恭之, 米澤奈津季, 武田大介, 高橋佑輔, 筧 康正, Akashi Masaya, Minamikawa Tsutomu, Furudoi Shungo, Hashikawa Kazunobu, Komori Takahide

  第34回日本口腔腫瘍学会総会・学術大会, Jan. 2016, Japanese, 日本口腔腫瘍学会, 横浜, Domestic conference

  Poster presentation


• 口腔扁平上皮癌における外的刺激受容体（TRPVチャネル）の発現

  Sakakibara Akiko, 榊原 俊介, 楠元 順哉, 武田 大介, Hasegawa Takumi, Akashi Masaya, Minamikawa Tsutomu, Hashikawa Kazunobu, Komori Takahide

  第34回日本口腔腫瘍学会総会・学術大会, Jan. 2016, Japanese, 日本口腔腫瘍学会総会・学術大会, 横浜市, Domestic conference

  Oral presentation


• 口腔扁平上皮癌における外的刺激受容体(TRPVチャネル)の発現亢進

  Sakakibara Akiko, 榊原俊介, 楠元順哉, 武田大介, Hasegawa Takumi, Akashi Masaya, Minamikawa Tsutomu, Hashikawa Kazunobu, Komori Takahide

  第34回日本口腔腫瘍学会総会・学術大会, Jan. 2016, Japanese, 日本口腔腫瘍学会, 横浜, Domestic conference

  Poster presentation


• 局所的炭酸ガス投与による皮弁壊死抑制効果

  齊藤 泉, Hasegawa Takumi, 武田大介, 岩田英治, 有本智美, 筧康正, 高橋佑輔, Sakakibara Akiko, Akashi Masaya, Minamikawa Tsutomu, Komori Takahide

  第34回日本口腔腫瘍学会総会・学術大会, Jan. 2016, Japanese, 日本口腔腫瘍学会, 横浜, Domestic conference

  Poster presentation


• 移植腓骨と下顎骨の癒合における骨膜温存の重要性（ポスター発表）

  Akashi Masaya, 筧 康正, Sakakibara Akiko, Hasegawa Takumi, Minamikawa Tsutomu, Hashikawa Kazunobu, Komori Takahide

  第34回日本口腔腫瘍学会総会・学術大会, Jan. 2016, Japanese, 日本口腔腫瘍学会, 横浜, Domestic conference

  Poster presentation


• 移植腓骨と下顎骨の癒合における骨膜温存の重要性

  Akashi Masaya, 筧 康正, Sakakibara Akiko, Hasegawa Takumi, Minamikawa Tsutomu, Hashikawa Kazunobu, Komori Takahide

  第34回日本口腔腫瘍学会総会・学術大会, Jan. 2016, Japanese, 日本口腔腫瘍学会総会・学術大会, 横浜市, Domestic conference

  Oral presentation


• 再建プレート露出に対する二期再建術前PET/CTが膿瘍の検知に有用であった1例

  松尾 健司, Akashi Masaya, 楠元 順哉, 藤林 淳子, 渡邊 一博, Minamikawa Tsutomu, Hashikawa Kazunobu, 古森 孝英

  第27回NPO法人日本口腔科学会近畿地方部会．, Nov. 2015, Japanese, 日本口腔外科学会, 大阪, Domestic conference

  Oral presentation


• 化学放射線療法による口腔粘膜炎の重症度を予測する因子についての検討

  岸本 恵実, Furudoi Shungo, 藤林 淳子, 西井 美佳, 岩田 英治, 楠元 順哉, 梶 真人, 後藤 育子, Akashi Masaya, 古森 孝英

  第24回日本口腔感染症学会総会．, Nov. 2015, Japanese, 日本口腔感染症学会, 神奈川, Domestic conference

  Oral presentation


• Targeted Disruption of JCAD/KIAA1462, a Coronary Artery Disease-associated Gene Product, Inhibits Angiogenic Processes in Vitro and in Vivo.

  Tetsuya Hara, Tomoko Monguchi, Akashi Masaya, Noriko Iwamoto, Kenta Mori, Toh Ryuji, Irino Yasuhiro, Shinohara Masakazu, Mikio Furuse, Ishida Tatsuro, Hirata Ken-ichi

  American Heart Association Scientic Session 2015., Nov. 2015, English, American Heart Association, Orlando, USA, Background: Recent genome-wide association studies newly identified the human KIAA1462 gene as a new locus for coronary artery disease. However, the function of the gene product, named JCAD (junctional protein associated with coronary artery disease), is, International conference

  Poster presentation


• 入院管理下で口腔外科小手術を施行した患者に関する臨床統計

  棚倉 万紀子, 筧 康正, Minamikawa Tsutomu, 高橋 佑輔, 米澤 奈津季, Sakakibara Akiko, Hasegawa Takumi, Akashi Masaya, 古森 孝英

  第60回日本口腔外科学会総会, Oct. 2015, Japanese, 日本口腔外科学会, 名古屋, Domestic conference

  Oral presentation


• 超音波断層法による口腔癌頸部リンパ節転移の検討

  筧 康正, Minamikawa Tsutomu, 棚倉 万紀子, 米澤 奈津季, 高橋 佑輔, 松井 太輝, Hasegawa Takumi, Akashi Masaya, 重田 崇至, 古森 孝英

  第60回日本口腔外科学会総会, Oct. 2015, Japanese, 日本口腔外科学会, 名古屋, Domestic conference

  Oral presentation


• 進展例の放射線性顎骨壊死の外科的治療におけるPETの有用性.

  Akashi Masaya, Hashikawa Kazunobu, Minamikawa Tsutomu, Furudoi Shungo, Komori Takahide

  第60回日本口腔外科学会総会．, Oct. 2015, Japanese, 日本口腔外科学会, 名古屋, Domestic conference

  Oral presentation


• 三次元実態模型を用いた手術シミュレーションー当科における最近の使用経験

  鰐渕 聡, Akashi Masaya, Hashikawa Kazunobu, 筧 康正, 松本 耕祐, Kimoto Akira, Hasegawa Takumi, Minamikawa Tsutomu, Furudoi Shungo, 古森 孝英

  第60回日本口腔外科学会総会, Oct. 2015, Japanese, 日本口腔外科学会, 名古屋, Domestic conference

  Oral presentation


• 口腔扁平上皮癌における外的刺激受容体の発現

  Sakakibara Akiko, 楠元 順哉, Akashi Masaya, Minamikawa Tsutomu, Komori Takahide

  第60回日本口腔外科学会, Oct. 2015, Japanese, 日本口腔外科学会, 名古屋, Domestic conference

  Oral presentation


• 口腔癌原発巣の術前18F-FDG PET/CT撮影に関する検討

  米澤 奈津季, Minamikawa Tsutomu, 北島一宏, 高橋 佑輔, 筧 康正, Sakakibara Akiko, Hasegawa Takumi, Akashi Masaya, 古森 孝英

  第60回日本口腔外科学会総会, Oct. 2015, Japanese, 日本口腔外科学会, 名古屋, Domestic conference

  Oral presentation


• 経皮的炭酸ガス投与による口腔癌上皮間葉移行の抑制効果

  岩田 英治, Hasegawa Takumi, 武田 大介, Akashi Masaya, Minamikawa Tsutomu, 古森 孝英

  第60回日本口腔外科学会総会, Oct. 2015, Japanese, 日本口腔外科学会, 名古屋, Domestic conference

  Oral presentation


• ロケーター・マグネット付きカスタムバーによるインプラントオーバーデンチャー関連合併症

  有本 智美, Hasegawa Takumi, 斉藤 泉, 岩田 英治, 武田 大介, Akashi Masaya, Suzuki Yasuaki, Furudoi Shungo, 古森 孝英

  第60回日本口腔外科学会総会．, Oct. 2015, Japanese, 日本口腔外科学会, 名古屋, Domestic conference

  Oral presentation


• Observation of Mastication in Patients Underwent Mandibular Reconstruction by 4D-CT with 320 Detector-Row Scanning : A Preliminary Report,

  Akashi Masaya, Shibuya Y, Takahashi Satoru, Hashikawa Kazunobu, Hasegawa Takumi, Kakei Y, Negi N, Sekitani T, Komori Takahide

  22nd International conference on oral and maxilofacial surgery,, Oct. 2015, English, メルボルン, オーストラリア, International conference

  Oral presentation


• Incidence of venousthromboembolism after oral surgery with simultaneous reconstruction,

  Kakei Y, Akashi Masaya, Shigeta T, Takahashi Yutaka, Mastui T, Sakakibara Akiko, Hasegawa Takumi, Minamikawa Tsutomu, Komori Takahide

  22nd International conference on oral and maxilofacial surgery,, Oct. 2015, English, メルボルン, オーストラリア, International conference

  Oral presentation


• Evaluating the changes in the amount of bone augmentation after sinus lift using panoramic radiography: Comparison evaluations based on CT images．

  松尾 健司, Suzuki Yasuaki, 松本 耕祐, Kimoto Akira, Hasegawa Takumi, Akashi Masaya, 竹内 順一郎, 古森 孝英

  第60回日本口腔外科学会総会．, Oct. 2015, Japanese, 日本口腔外科学会, 名古屋, Domestic conference

  Oral presentation


• A novel strategy aimed at apoptosis via the mitochondrial pathway and improving hypoxia in oral squamous cell carcinoma.．

  武田 大介, Hasegawa Takumi, Sakakibara Akiko, Akashi Masaya, Minamikawa Tsutomu, 古森 孝英

  第60回日本口腔外科学会総会, Oct. 2015, Japanese, 日本口腔外科学会, 名古屋, Domestic conference

  Oral presentation


• 当科における下顎の骨折線上の歯に関する臨床的検討

  北山 美登里, Suzuki Yasuaki, 小守 紗也華, 松本 耕祐, Akashi Masaya, 古森 孝英

  第15回日本外傷歯学会，, Jul. 2015, Japanese, 日本外傷歯学会, 福岡, Domestic conference

  Oral presentation


• 混合歯列期の下顎骨骨折に対し非観血的整復治療を行った1例

  小守 紗也華, 松本 耕祐, 北山 美登里, Akashi Masaya, 立石 千鶴, Suzuki Yasuaki, Komori Takahide

  第15回日本外傷歯学会，, Jul. 2015, Japanese, 日本外傷歯学会, 福岡, Domestic conference

  Oral presentation


• 当院における周術期口腔機能管理に関する実態調査と今後の課題

  田村 惠利, 西井 美佳, 冨田 和, 難波 渚, Hasegawa Takumi, Akashi Masaya, Furudoi Shungo, 古森 孝英

  第12回日本口腔ケア学会総会・学術大会．, Jun. 2015, Japanese, 日本口腔ケア学会, 山口, Domestic conference

  Oral presentation


• 外科的矯正手術前後における顎関節症状および顎関節MRI画像の変化.

  定兼 啓倫, Hasegawa Takumi, 有本 智美, 岡本 奈那, 塩屋園 敦, 立石 千鶴, Akashi Masaya, Suzuki Yasuaki, Furudoi Shungo, Komori Takahide

  第25回日本顎変形症学会総会．, Jun. 2015, Japanese, 日本顎変形症学会, 東京, Domestic conference

  Oral presentation


• Sequential evaluation of bone union of transferred fibula flaps in reconstructed mandibles.

  Akashi Masaya, Hashikawa Kazunobu, Kakei Y, Sakakibara Akiko, Hasegawa Takumi, Minamikawa Tsutomu, Komori Takahide

  "The Joint Meeting of 4th Congress of Asian Society of Head and Neck Oncology & 39th Annual Meeting of Japan society for Head and Neck Cancer．, Jun. 2015, English, 神戸, Domestic conference

  Oral presentation


• Decreased mitochondrial copy numbers in oral squamous cell carcinoma.

  Takeda D, Hasegawa Takumi, Sakakibara Akiko, Akashi Masaya, Minamikawa Tsutomu, Komori Takahide

  "The Joint Meeting of 4th Congress of Asian Society of Head and Neck Oncology & 39th Annual Meeting of Japan society for Head and Neck Cancer．, Jun. 2015, English, 神戸, Domestic conference

  Oral presentation


• -

  難波 渚, 西井 美佳, 冨田 和, 田村 恵利, Hasegawa Takumi, Akashi Masaya, Furudoi Shungo, 古森 孝英

  第12回日本口腔ケア学会総会・学術大会．, Jun. 2015, Japanese, 日本口腔ケア学会, 山口, Domestic conference

  Oral presentation


• 下顎骨骨髄炎との鑑別が困難であった下顎歯肉悪性腫瘍の1例

  渡邊 一博, Furudoi Shungo, 筧 康正, Akashi Masaya, Minamikawa Tsutomu, 古森 孝英

  第46回日本口腔外科学会近畿支部学術集会．, Mar. 2015, Japanese, 日本口腔外科学会, 神戸, Domestic conference

  Oral presentation


• 移植非骨と下顎骨の骨癒合に関する経時的評価－パノラマX線とCT画像の比較－

  Akashi Masaya, Hashikawa Kazunobu, Sakakibara Akiko, 楠元 順哉

  第109回関西形成外科学会地方会．, Mar. 2015, Japanese, 関西形成外科学会, 京都, Domestic conference

  Oral presentation


• 当科における頸部郭清術術後の頸部ドレーンに関する臨床的検討

  齊藤 泉, Hasegawa Takumi, 米澤 奈津季, 武田 大介, 高橋 佑輔, 筧 康正, 松井 太輝, Sakakibara Akiko, Akashi Masaya, Minamikawa Tsutomu, 古森 孝英

  第33回日本口腔腫瘍学会, Jan. 2015, Japanese, 日本口腔腫瘍学会, 奈良, Domestic conference

  Oral presentation


• 当科における放射線性顎骨壊死に関する臨床的検討

  鰐渕 聡, Akashi Masaya, 筧 康正, Sakakibara Akiko, Hasegawa Takumi, Minamikawa Tsutomu, 古森 孝英

  第33回日本口腔腫瘍学会, Jan. 2015, Japanese, 日本口腔腫瘍学会, 奈良, Domestic conference

  Oral presentation


• 当科における口腔扁平上皮癌遠隔転移例の臨床的検討とリスク因子について

  棚倉 万紀子, Hasegawa Takumi, 米澤 奈津季, 武田 大介, 高橋 佑輔, 筧 康正, Sakakibara Akiko, Akashi Masaya, Minamikawa Tsutomu, 古森 孝英

  第33回日本口腔腫瘍学会, Jan. 2015, Japanese, 日本口腔腫瘍学会, 奈良, Domestic conference

  Oral presentation


• 口腔扁平上皮癌臨床検体におけるミトコンドリア量について

  武田 大介, Hasegawa Takumi, 今井 佑輔, Sakakibara Akiko, Akashi Masaya, Minamikawa Tsutomu, 古森 孝英

  第33回日本口腔腫瘍学会, Jan. 2015, Japanese, 日本口腔腫瘍学会, 奈良, Domestic conference

  Poster presentation


• 口腔癌手術症例におけるmodified Glasgow Prognostic Score(mGPS)を用いた長期予後に関する検討

  平岡 佑二郎, Minamikawa Tsutomu, 米澤 奈津季, 高橋 佑輔, Sakakibara Akiko, Hasegawa Takumi, Akashi Masaya, 古森 孝英

  第33回日本口腔腫瘍学会, Jan. 2015, Japanese, 日本口腔腫瘍学会, 奈良, Domestic conference

  Poster presentation


• 口腔癌原発巣におけるDual-time-point FDG PETの有用性

  米澤 奈津季, Minamikawa Tsutomu, 高橋 佑輔, 筧 康正, 松井 太輝, Sakakibara Akiko, Hasegawa Takumi, Akashi Masaya, 古森 孝英

  第33回日本口腔腫瘍学会, Jan. 2015, Japanese, 日本口腔腫瘍学会, 奈良, Domestic conference

  Oral presentation


• 下顎枝垂直骨切り術後の近位骨片と下顎頭変化の評価

  有本 智美, Hasegawa Takumi, 定兼 啓倫, 岡本 奈那, 塩屋園 敦, 立石 千鶴, Akashi Masaya, Suzuki Yasuaki, Furudoi Shungo, Komori Takahide

  第25回日本顎変形症学会総会．, Jan. 2015, Japanese, 日本顎変形症学会, 東京, Domestic conference

  Oral presentation


• 放射線治療後の甲状腺機能低下による低ナトリウム血症の1例

  村田 陽太郎, Hasegawa Takumi, 八橋 明子, Akashi Masaya, Minamikawa Tsutomu, Furudoi Shungo, Komori Takahide

  第26回日本口腔科学会近畿地方部会, Dec. 2014, Japanese, 日本口腔科学会, 京都, Domestic conference

  Oral presentation


• 歯科インプラント埋入のためのCT画像を用いた移植腓骨の形態評価

  Akashi Masaya, Hashikawa Kazunobu, Sakakibara Akiko, 楠元 順哉

  第108回関西形成外科学会学術集会, Dec. 2014, Japanese, 関西形成外科学会, 大阪, Domestic conference

  Oral presentation


• 経口的ビタミンB12投与が著効したHUNTER舌炎の1例

  中田 和甫史, 若林 玲奈, Akashi Masaya, Komori Takahide

  第26回日本口腔科学会近畿地方部会, Dec. 2014, Japanese, 日本口腔科学会, 京都, Domestic conference

  Oral presentation


• 当科における広範囲顎骨支持型インプラント補綴の臨床統計的検討

  今井 佑輔, Hasegawa Takumi, 川畑 真哉, 武田 大介, Akashi Masaya, Suzuki Yasuaki, Komori Takahide

  第18回日本顎顔面インプラント学会学術大会, Nov. 2014, Japanese, 日本顎顔面インプラント学会, 島根, Domestic conference

  Oral presentation


• 当科におけるインプラント242例907本埋入に関する臨床的検討

  川畑 真哉, Hasegawa Takumi, 今井 佑輔, Kimoto Akira, Akashi Masaya, Suzuki Yasuaki, Komori Takahide

  第18回日本顎顔面インプラント学会学術大会, Nov. 2014, Japanese, 日本顎顔面インプラント学会, 島根, Domestic conference

  Oral presentation


• 著明な上顎洞粘膜肥厚を有する患者に洞底挙上術後インプラント治療を行った症例

  榎本 由依, Suzuki Yasuaki, Kimoto Akira, Hasegawa Takumi, Akashi Masaya, 竹内 純一郎, Komori Takahide

  第18回日本顎顔面インプラント学会学術大会, Nov. 2014, Japanese, 日本顎顔面インプラント学会, 島根, Domestic conference

  Oral presentation


• 小児の顔面多発骨折症例の経験

  大﨑 健夫, 川北 育子, 北野 豊明, 桒水流 健二, Akashi Masaya

  第32回日本頭蓋顎顔面外科学会学術集会, Nov. 2014, Japanese, 日本頭蓋顎顔面外科学会, 大阪, Domestic conference

  Oral presentation


• 歯科インプラント埋入のためのCT画像を用いた移植腓骨の形態評価

  Akashi Masaya, Shibuya Yasuyuki, 鰐渕 聡, Kimoto Akira, Hasegawa Takumi, Suzuki Yasuaki, Komori Takahide

  第18回日本顎顔面インプラント学会学術大会, Nov. 2014, Japanese, 日本顎顔面インプラント学会, 島根, Domestic conference

  Oral presentation


• 入院加療を要した口腔頸部の重症感染症例の検討

  藤林 淳子, Furudoi Shungo, 畑 みどり, 楠元 順哉, 後藤 育子, Akashi Masaya, Komori Takahide

  第23回日本口腔感染症学会総会, Oct. 2014, Japanese, 日本口腔感染症学会, 伊丹, Domestic conference

  Oral presentation


• 頭頸部領域における壊死性筋膜炎に対するLRINECスコアの有用性の検討

  楠元 順哉, Furudoi Shungo, Akashi Masaya, Sakakibara Akiko, Komori Takahide

  第59回日本口腔外科学会総会, Oct. 2014, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Oral presentation


• 当科における口腔癌患者の術後せん妄に関する臨床的検討

  齊藤 泉, Hasegawa Takumi, 米澤 奈津季, 高橋 佑輔, 筧 康正, 松井 太輝, Sakakibara Akiko, Akashi Masaya, Minamikawa Tsutomu, Komori Takahide

  第59回日本口腔外科学会総会, Oct. 2014, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Oral presentation


• 当院における心臓血管外科患者における周術期口腔機能管理

  藤 大補, Akashi Masaya, Furudoi Shungo, 西井 美佳, 後藤 育子, Komori Takahide

  第23回日本口腔感染症学会総会, Oct. 2014, Japanese, 日本口腔感染症学会, 伊丹, Domestic conference

  Oral presentation


• 低出力超音波パルスの下顎骨骨折血腫細胞のBMP発現における効果

  黄 文蘇, Hasegawa Takumi, 今井 佑輔, 武田 大介, Akashi Masaya, Komori Takahide

  第59回日本口腔外科学会総会, Oct. 2014, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Poster presentation


• 上顎部分切除後の遊離前腕皮弁再建群と非再建群の比較

  Akashi Masaya, Shibuya Yasuyuki, 楠元 順哉, Sakakibara Akiko, Hasegawa Takumi, Minamikawa Tsutomu, Furudoi Shungo, Hashikawa Kazunobu, Komori Takahide

  第59回日本口腔外科学会総会, Oct. 2014, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Oral presentation


• 抗RANKLモノクローナル抗体（デノスマブ）に発症した顎骨壊死の1例

  岩田 英治, Akashi Masaya, Hasegawa Takumi, Furudoi Shungo, Komori Takahide

  第23回日本口腔感染症学会総会, Oct. 2014, Japanese, 日本口腔感染症学会, 伊丹, Domestic conference

  Oral presentation


• 口腔扁平上皮癌N0症例への予防的頸部郭清の検討：T1-2とT3-4の比較

  八橋 明子, Minamikawa Tsutomu, 松井 太輝, 高橋 佑輔, 米澤 奈津季, 筧 康正, Sakakibara Akiko, Hasegawa Takumi, Akashi Masaya, Komori Takahide

  第59回日本口腔外科学会総会, Oct. 2014, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Poster presentation


• 口腔癌手術における輸血療法の現状

  米澤 奈津季, Minamikawa Tsutomu, 高橋 佑輔, 筧 康正, 松井 太輝, Sakakibara Akiko, Hasegawa Takumi, Akashi Masaya, Shibuya Yasuyuki, Komori Takahide

  第59回日本口腔外科学会総会, Oct. 2014, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Oral presentation


• 口腔癌に対する救済手術についての検討

  筧 康正, Minamikawa Tsutomu, 米澤 奈津季, 高橋 佑輔, 松井 太輝, Sakakibara Akiko, Hasegawa Takumi, Akashi Masaya, Shibuya Yasuyuki, Komori Takahide

  第59回日本口腔外科学会総会, Oct. 2014, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Oral presentation


• 局所的炭酸ガス投与による口腔癌新規治療法の開発

  武田 大介, Hasegawa Takumi, 今井 佑輔, Sakakibara Akiko, Akashi Masaya, Minamikawa Tsutomu, Shibuya Yasuyuki, Komori Takahide

  第59回日本口腔外科学会総会, Oct. 2014, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Poster presentation


• 化学放射線療法中の中咽頭癌患者における口腔乾燥の経時的変化

  西井 美佳, Akashi Masaya, 筧 康正, Hasegawa Takumi, Minamikawa Tsutomu, Furudoi Shungo, Shibuya Yasuyuki, Komori Takahide

  第59回日本口腔外科学会総会, Oct. 2014, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Oral presentation


• フェーズラグCT（pl-CTA）による顎骨周囲の脈管の評価

  Sakakibara Akiko, Sakakibara Shunsuke, Hasegawa Takumi, Akashi Masaya

  第59回日本口腔外科学会総会・学術集会, Oct. 2014, Japanese, 日本口腔外科学会, 幕張, Domestic conference

  Oral presentation


• フェーズラグCTA（ｐｌ－CTA）による顎動静脈分岐の評価

  Sakakibara Akiko, Sakakibara Shunsuke, 楠元 順哉, Hasegawa Takumi, Akashi Masaya, Suzuki Yasuaki, Minamikawa Tsutomu, Furudoi Shungo, Hashikawa Kazunobu, Komori Takahide

  第59回日本口腔外科学会総会, Oct. 2014, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Oral presentation


• ビスフォスフォネート関連顎骨壊死症例のCT画像における臨床的検討

  岩田 英治, Akashi Masaya, 楠元 順哉, Hasegawa Takumi, 後藤 育子, Furudoi Shungo, Komori Takahide

  第59回日本口腔外科学会総会, Oct. 2014, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Poster presentation


• 対側オトガイ部の知覚異常を初発症状としたびまん性大細胞型B細胞性リンパ腫の1例

  高端 大希, Akashi Masaya, 高橋 佑輔, 辻 和志, 西尾 良介, Minamikawa Tsutomu, Komori Takahide

  第45回日本口腔外科学会近畿地方会, Jun. 2014, Japanese, 日本口腔外科学会, 奈良, Domestic conference

  Oral presentation


• 口腔癌再建術後の感染性合併症－術後CRPのabnormal responseに注目して－

  Akashi Masaya, Hashikawa Kazunobu, Minamikawa Tsutomu, Shibuya Yasuyuki, Komori Takahide

  第38回日本頭頸部癌学会, Jun. 2014, Japanese, 日本頭頸部癌学会, 東京, Domestic conference

  Oral presentation


• 顎関節部に限局する線維-骨性病変の1例

  八橋 明子, Shibuya Yasuyuki, 後藤 育子, Akashi Masaya, 松井 太輝, Komori Takahide

  第45回日本口腔外科学会近畿地方会, Jun. 2014, Japanese, 日本口腔外科学会, 奈良, Domestic conference

  Oral presentation


• 過去15年間の高齢口腔癌患者に関する臨床的検討

  米澤 奈津季, Minamikawa Tsutomu, 高橋 佑輔, Akashi Masaya, Shibuya Yasuyuki, Komori Takahide

  第38回日本頭頸部癌学会, Jun. 2014, Japanese, 日本頭頸部癌学会, 東京, Domestic conference

  Oral presentation


• 栄養・輸液サポートチームにおける歯科的介入について

  若林 玲奈, Shibuya Yasuyuki, 今井 佑輔, 黒木 信祐, 後藤 育子, Akashi Masaya, Furudoi Shungo, Komori Takahide

  第45回日本口腔外科学会近畿地方会, Jun. 2014, Japanese, 日本口腔外科学会, 奈良, Domestic conference

  Oral presentation


• 造血気悪性腫瘍患者への歯科的介入に関する前向き検討

  辻 和志, Shibuya Yasuyuki, 岸本 恵実, 棚倉 万紀子, Akashi Masaya, Furudoi Shungo, Komori Takahide

  第68回日本口腔科学会学術総会, May 2014, Japanese, 日本口腔科学会, 東京, Domestic conference

  Oral presentation


• 口腔内細菌数が放射線性口腔粘膜炎に及ぼす影響について

  後藤 育子, Furudoi Shungo, 西井 美佳, 楠本 順哉, 梶 真人, Akashi Masaya, Komori Takahide

  第68回日本口腔科学会学術総会, May 2014, Japanese, 日本口腔科学会, 東京, Domestic conference

  Poster presentation


• 口腔癌再建術後の頸部感染評価目的でのCTの有用性

  Akashi Masaya, Furudoi Shungo, Minamikawa Tsutomu, Kimoto Akira, Sakakibara Akiko, Hasegawa Takumi, 後藤 育子, Shibuya Yasuyuki, Komori Takahide

  第68回日本口腔科学会学術総会, May 2014, Japanese, 日本口腔科学会, 東京, Domestic conference

  Oral presentation


• Preformed Reconstruction plateで下顎再建を行った2症例

  竹内 惇平, Furudoi Shungo, 藤 大補, 高橋 佑輔, Akashi Masaya, Sakakibara Akiko, Minamikawa Tsutomu, Shibuya Yasuyuki, Komori Takahide

  第68回日本口腔科学会学術総会, May 2014, Japanese, 日本口腔科学会, 東京, Domestic conference

  Oral presentation


• パネルディスカッション3. ビデオで伝えたい私の手順

  Nomura Tadashi, Sakakibara Shunsuke, Takasu Hiroyuki, Akashi Masaya, Hashikawa Kazunobu, 田原 真也, Terashi Hiroto

  第19回日本形成外科手術手技学会, Feb. 2014, Japanese, 日本形成外科手術手技学会, 愛知, Domestic conference

  Public discourse


• 高齢口腔癌患者に関する臨床的検討

  米澤 奈津季, Minamikawa Tsutomu, Akashi Masaya, 高橋 佑輔, 重田 崇至, Shibuya Yasuyuki, Komori Takahide

  第32回日本口腔腫瘍学会, Jan. 2014, Japanese, 日本口腔腫瘍学会, 札幌, Domestic conference

  Oral presentation


• 口腔癌cN1症例に対する頸部郭清術の廓清範囲についての検討

  筧 康正, Minamikawa Tsutomu, 高橋 佑輔, 松井 太輝, Hasegawa Takumi, Akashi Masaya, 重田 崇至, Furudoi Shungo, Shibuya Yasuyuki, Komori Takahide

  第32回日本口腔腫瘍学会, Jan. 2014, Japanese, 日本口腔腫瘍学会, 札幌, Domestic conference

  Oral presentation


• 遊離腓骨皮弁移植におけるMR Angiographyによる下肢血管解剖の術前評価

  楠元 順哉, 橋川 和信, Akashi Masaya, 榊原 晶子, Terashi Hiroto

  第105回日本形成外科学会関西支部学術集会, Dec. 2013, Japanese, 本形成外科学会, 大阪, Domestic conference

  Oral presentation


• 口腔外に肉芽形成をきたしたビスフォスフォネート関連顎骨壊死の1例

  岩田英治, Furudoi Shungo, 楠元順哉, 後藤育子, Akashi Masaya, Komori Takahide

  第25回日本口腔科学会近畿地方部会, Dec. 2013, Japanese, 日本口腔科学会, 大阪, Domestic conference

  Oral presentation


• 胃癌術後の腹壁再発切除術により生じた 胸腹壁欠損に対する治療経験

  長谷川 泰子, Nomura Tadashi, Akashi Masaya, 前田 翔, 橋川 和信, Terashi Hiroto

  第105回関西形成外科学会学術集会, Dec. 2013, Japanese, 関西形成外科学会学術集会, 大阪市, Domestic conference

  Oral presentation


• 片側性唇顎口蓋裂を伴う咬合不全に対し腓骨皮弁による骨再建とインプラント埋入術を行った成人男性の1例

  齊藤 泉, Shibuya Yasuyuki, 筧 康正, Akashi Masaya, Shigeta Takashi, Komori Takahide

  第17回顎顔面インプラント学会総会, Nov. 2013, Japanese, 顎顔面インプラント学会, 東京, Domestic conference

  Oral presentation


• 当科における重症性感染症例の臨床的検討

  藤林淳子, Furudoi Shungo, 小松原秀紀, 楠元順哉, 後藤育子, Akashi Masaya, Komori Takahide

  第22回口腔感染症学会総会, Nov. 2013, Japanese, 口腔感染症学会, 八王子, Domestic conference

  Oral presentation


• 呼吸サポートチームへの歯科介入がVAP予防に与える影響について

  後藤育子, Furudoi Shungo, 西井美佳, 四宮麻美, 楠元順哉, 梶 真人, Akashi Masaya, Shibuya Yasuyuki, Komori Takahide

  第22回口腔感染症学会総会, Nov. 2013, Japanese, 口腔感染症学会, 八王子, Domestic conference

  Oral presentation


• 超高純度マグネシウムを用いて生体吸収性金属製骨接合プレートを作製する

  橋川 和信, Takasu Hiroyuki, Akashi Masaya, Terashi Hiroto

  第31回日本頭蓋顎顔面外科学会学術集会, Oct. 2013, Japanese, 日本頭蓋顎顔面外科学会学術集会, 名古屋市, Domestic conference

  Oral presentation


• 舌半側切除後の遊離前腕皮弁再建症例における長期観察結果．

  Akashi Masaya, 橋川 和信, 榊原 晶子, Hasegawa Takumi, Shigeta Takashi, Minamikawa Tsutomu, Furudoi Shungo, Shibuya Yasuyuki, Komori Takahide

  第58回日本口腔外科学会総会・学術大会, Oct. 2013, Japanese, 日本口腔外科学会総会・学術大会, 福岡市, Domestic conference

  Oral presentation


• 口腔癌患者における胃瘻造設に関する検討―術中同時胃瘻造設について-

  米澤奈津季, Minamikawa Tsutomu, Akashi Masaya, 高橋佑輔, Hasegawa Takumi, 榊原晶子, Shigeta Takashi, Shibuya Yasuyuki, Komori Takahide

  第58回日本口腔外科学会総会, Oct. 2013, Japanese, 日本口腔外科学会, 福岡, Domestic conference

  Oral presentation


• 口腔癌における周術期静脈血栓塞栓症について

  筧 康正, Minamikawa Tsutomu, 高橋佑輔, 松井太輝, 榊原晶子, Akashi Masaya, Shigeta Takashi, Furudoi Shungo, Shibuya Yasuyuki, Komori Takahide

  第58回日本口腔外科学会総会, Oct. 2013, Japanese, 日本口腔外科学会, 福岡, Domestic conference

  Oral presentation


• シスプラチン併用化学放射線療法の術後補助療法としての有効性に関する検討

  Shigeta Takashi, Minamikawa Tsutomu, 松井太輝, Akashi Masaya, Hasegawa Takumi, 高橋佑輔, 筧 康正, Shibuya Yasuyuki, 梅田正博, Komori Takahide

  第58回日本口腔外科学会総会, Oct. 2013, Japanese, 日本口腔外科学会, 福岡, Domestic conference

  Oral presentation


• 再発口腔中咽頭癌に対する遊離皮弁再建に関する検討．

  Akashi Masaya, 橋川 和信, Terashi Hiroto

  第40回日本マイクロサージャリー学会学術集会, Sep. 2013, Japanese, 日本マイクロサージャリー学会学術集会, 盛岡市, Domestic conference

  Oral presentation


• 再発口腔中咽頭癌に対する遊離皮弁再建における問題点-第2報-

  Akashi Masaya, 橋川和信, Terashi Hiroto

  第40回日本マイクロサージェリー学会総会, Sep. 2013, Japanese, 日本マイクロサージェリー学会, 岩手, Domestic conference

  Oral presentation


• 舌半側切除後の遊離前腕皮弁再建症例における長期観察結果．

  Akashi Masaya, 橋川 和信, 榊原 晶子, Terashi Hiroto

  第37回日本頭頸部癌学会, Jun. 2013, Japanese, 日本頭頸部癌学会, 東京都, Domestic conference

  Oral presentation


• 再発口腔中咽頭癌に対する遊離皮弁再建における問題点

  Akashi Masaya, 橋川 和信, Terashi Hiroto

  第37回日本頭頸部癌学会総会, Jun. 2013, Japanese, 日本頭頸部癌学会, 東京, Domestic conference

  Oral presentation


• がん切除後下顎再建における再建理論「CATコンセプト」．

  橋川 和信, Akashi Masaya, Nomura Tadashi, Terashi Hiroto

  第37回日本頭頸部癌学会, Jun. 2013, Japanese, 日本頭頸部癌学会, 東京都, Domestic conference

  Oral presentation


• 造血器悪性腫瘍患者への歯科的介入に関する前向き検討

  辻 和志, Shibuya Yasuyuki, Akashi Masaya, Furudoi Shungo, Komori Takahide

  第67回日本口腔科学会総会, May 2013, Japanese, 日本口腔科学会, 宇都宮, Domestic conference

  Oral presentation


• 放射線誘発癌が疑われた口腔癌に対する遊離皮弁再建

  Akashi Masaya, 橋川 和信, Terashi Hiroto

  第103回関西形成外科学会学術集会, Mar. 2013, Japanese, 関西形成外科学会学術集会, 大阪市, Domestic conference

  Oral presentation


• 舌癌180例のリンパ節転移に関する検討～健側転移について～

  松井太輝, Shigeta Takashi, Minamikawa Tsutomu, Akashi Masaya, 高橋佑輔, 筧 康正, Furudoi Shungo, Shibuya Yasuyuki, Komori Takahide

  第31回日本口腔腫瘍学会総会, Jan. 2013, Japanese, 日本口腔腫瘍学会, 東京, Domestic conference

  Oral presentation


• 口腔扁平上皮癌術後局所再発症例に関する検討

  高橋佑輔, Minamikawa Tsutomu, 筧 康正, 松井太輝, 榊原晶子, Akashi Masaya, Shigeta Takashi, Shibuya Yasuyuki, Komori Takahide

  第31回日本口腔腫瘍学会総会, Jan. 2013, Japanese, 日本口腔腫瘍学会, 東京, Domestic conference

  Oral presentation


• 口腔扁平上皮癌T4症例に対する手術療法の治療成績

  筧 康正, Minamikawa Tsutomu, 高橋佑輔, 松井太輝, 榊原晶子, Akashi Masaya, Shigeta Takashi, Furudoi Shungo, Shibuya Yasuyuki, Komori Takahide

  第31回日本口腔腫瘍学会総会, Jan. 2013, Japanese, 日本口腔腫瘍学会, 東京, Domestic conference

  Oral presentation


• Facial artery musculomucosal flapにより口腔上顎洞瘻孔を治療した1例

  Akashi Masaya, 橋川 和信, Sakurai Sayuri, Terashi Hiroto

  第30回日本頭蓋顎顔面外科学会学術集会, Nov. 2012, Japanese, 日本頭蓋顎顔面外科学会, 豊中, Domestic conference

  Oral presentation


• 遊離腓骨皮弁移植におけるMR Angiographyと超音波検査による下肢血管解剖の術前評価

  Akashi Masaya, 橋川 和信, 榊原 晶子, Hasegawa Takumi, Shigeta Takashi, Minamikawa Tsutomu, Furudoi Shungo, Shibuya Yasuyuki, Komori Takahide

  第57回日本口腔外科学会総会・学術大会, Oct. 2012, Japanese, 日本口腔外科学会, 横浜, Domestic conference

  Oral presentation


• 下顎骨区域欠損に対する再建理論「CATコンセプト」の実践

  橋川 和信, Akashi Masaya, 榊原 晶子

  第57回日本口腔外科学会総会・学術大会, Oct. 2012, Japanese, 日本口腔外科学会, 横浜, Domestic conference

  Oral presentation


• 外傷後に生じたclaw toe変形に対し長趾屈筋移行術を施行した1例

  Takasu Hiroyuki, 橋川 和信, Akashi Masaya, Terashi Hiroto

  第101回日本形成外科学会関西支部学術集会, Jul. 2012, Japanese, 日本形成外科学会関西支部, 大阪, Domestic conference

  Oral presentation


• 金属製再建プレートによる下顎骨再建後の合併症について－下顎骨硬性再建に関する一考察

  Akashi Masaya, 橋川 和信, 榊原 晶子, Sakakibara Shunsuke, 横尾 聡, Terashi Hiroto, 田原 真也

  第55回日本形成外科学会総会・学術集会, Apr. 2012, Japanese, 日本形成外科学会, 東京, Domestic conference

  Oral presentation


• プレート破折後に遊離腓骨皮弁で下顎骨再建を行った1例

  Akashi Masaya, Hashikawa Kazunobu, 田原 真也

  第99回日本形成外科学会 関西支部学術集会, Dec. 2011, Japanese, 日本形成外科学会, 京都, Domestic conference

  Oral presentation


• Protective immune response by intranasal immunization with split-virion influenza A vaccine with adjuvant and whole-viron influenza A vaccine

  Okamoto S, Matsuoka Shozo, Yamada Hideto, Gomi Y, Okuno Y, Akagi T, Akashi Masaya, Mori Yasuko, Yamanishi K

  Cell Symposia: Influenza - Translating basic insights, Dec. 2010, English, Elsevier(Cell Press), Washington DC, アメリカ合衆国, International conference

  Oral presentation


• Impaired stratum corneum formation in claudin-1-deficient mice with defect in tight junction function

  Sugawara T, Akashi Masaya, Yuki T, Nakagawa N, Sakai S, Sugiyama Y, Inoue S, Furuse Mikio

  第35回日本研究皮膚科学会総会, Dec. 2010, English, 日本研究皮膚科学会, 和歌山, Domestic conference

  Oral presentation


• 口腔癌手術における嚥下改善手術に関する臨床的検討．

  Akashi Masaya, Minamikawa Tsutomu, 高橋英哲, 小國 晶子, Hashikawa Kazunobu, Furudoi Shungo, Shibuya Yasuyuki, 梅田 正博, Komori Takahide

  第55回日本口腔外科学会総会, Oct. 2010, Japanese, 日本口腔外科学会, 千葉, Domestic conference

  Oral presentation

• 好中球に着眼したシェーグレン症候群の新規病理分類提案と治療薬探索

  後藤 育子, 西村 啓佑, 重岡 学, 木本 明, 明石 昌也, 明石 健吾, 筧 康正

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2023 - 31 Mar. 2026


• 顎骨壊死に対する腐骨分離促進療法の探索

  明石 昌也, 重岡 学, 有本 智美, 木本 明, 長谷川 巧実, 筧 康正

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2023 - 31 Mar. 2026


• ミトコンドリアの正常化と癌関連線維芽細胞の再教育を目指した新規口腔癌治療法の開発

  長谷川 巧実, 榊原 晶子, 明石 昌也, 武田 大介

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2022 - 31 Mar. 2026


• 統合オミックス解析による口腔がん制御機構の解明と創薬標的の網羅的探索

  木本 明, 明石 昌也, 榎本 由依

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2021 - 31 Mar. 2024

  口腔がんの大部分は扁平上皮癌であり、標準的治療は手術とされている。また、補助療法として放射線治療、化学療法が併用されることが多いが、進行がんに対しては予後が悪く、制御が可能であった場合でも患者自身のQOLを著しく低下させる。そのため、根治治療を目的としながらも、可能な限り機能を温存することも口腔がんにおいては重要である。現在、口腔がんに対する化学療法は、シスプラチン(CDDP)や5-FU、ドセタキセルが使用されている。しかし、進行がんを制御するまでは至っておらず、副作用も多い。本研究では、統合オミックス解析を行い、口腔がんに特異的な代謝経路を探索する。また、それに関わる代謝酵素のうち、がんの増殖の制御に関わるものを導き出し、その阻害剤の効果を検討することで、将来的な口腔がんの新たな治療薬の開発をめざす。 今年度は、まずヒト由来口腔がん細胞株（OSC-19、HSC-3）のガスクロマトグラフィー質量分析を実施した。また、口腔がん患者の血漿メタボローム解析を実施し、得られたデータを統計ソフトを用いて多変量解析することで、口腔がん患者に特異的な代謝物の変動を探索している。次に、ヒト由来口腔がん細胞株と血漿メタボローム解析の結果と比較することで、特に著明な変化を示す代謝経路の探索を開始している。今後は、これらに加え、口腔がん生体材料のメタボローム解析を実施することで、さらに口腔がん特異的な代謝経路を絞っていく予定である。


• Development of a new evaluation method for chewing function applying basic food science and its ripple effect on oral flail

  渋谷 恭之, 高岡 素子, 桐田 忠昭, 梅田 正博, 栗田 浩, 明石 昌也, 前田 道徳

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Nagoya City University, 01 Apr. 2020 - 31 Mar. 2024

  総義歯装着者や口腔外科等で顎骨切除した患者等に対して咀嚼機能を評価することは、総義歯の調整を行ったり、術後の経過を評価したり、術後の食事ケアの内容を決定したりするうえで極めて重要である。また、オーラルフレイルのある高齢者に提供する栄養学的に適切な食事メニューを決めるためにも、咀嚼機能の評価は必要である。従来の咀嚼機能評価としては、佐藤らの総義歯咀嚼能率判定表が良く用いられている。この判定法は、総義歯装着者に対して100種類の食品について咀嚼に関するアンケート調査を行った結果に基づき、各食品の咀嚼難易度を咀嚼指数を定めた表によって咀嚼機能が評価される。このため、各食材を用いることにより簡便に咀嚼機能を評価することができる。しかしながら、総義歯装着者に対するアンケートという主観的な調査方法に基づいているため客観性に乏しく、咀嚼難易度の判定基準が不明確であるという問題があった。また、食品の調理方法や形態についても定められておらず、曖昧であるという問題もあった。さらには、食生活の変化により、現代ではあまり食されていないという食品も多く含まれているという問題があった。そこで総義歯咀嚼能率判定表に示された数多くの食材について物理的特性を測定機器を用いて解析し、総義歯咀嚼能率判定表における咀嚼指数との相関関係を調べた。その結果、測定値から得られた５つのパラメータ（硬さ、粘着性、付着性、凝集性及び咀嚼性）のうち咀嚼性のみが咀嚼指数との間で有意な相関関係を示すことを見出した。さらに、咀嚼性を決定するパラメータのうち、硬さ及び凝集性の値によってクラスター分析を行ったところ、判定基準が明確で客観性を有するいくつかのクラスターに分類できることを明らかとなった。これを指標に試料となるグミを開発中である。


• Development of treatment system for periodontal disease and oral cancer using bio-compatible titanium dioxide

  荻野 千秋, 明石 昌也, 西村 勇哉, 筧 康正

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), Kobe University, 09 Jul. 2021 - 31 Mar. 2023

  近年、口腔がんと口腔細菌叢（歯周病菌叢）との関連を示唆する科学的根拠が報告され、注目を集めている(Scientific Reports, 7, 11773 (2017)など)。本研究の目的は、口腔がん、および歯周病の原因となる歯周病菌叢に対して、同一手法で治療する方法を提案する。具体的には、我々が構築した「過酸化チタン粒子」(TiOx)の酸化力、およびその放射線照射法の適用性を適用・検証する事である。本研究の目的は、「過酸化チタン／放射線」照射法を、口腔がんや歯周病菌叢へと適用し、少ない放射線量においてもがん細胞、および細菌損傷に適用可能か検証を行う事である。この研究目的の遂行のために、以下に掲げる2つの項目について検討を行った。 [1] 植物成分でコーティングされた過酸化チタン粒子の開発：TiO2 NPs(石原産業)を元に、RutinG-TiOx NPsと、PAA-TiOx NPsにRutinGを添加したRutinG+PAA-TiOx NPsを作製した。各修飾粒子をUV-visとFT-IRによって、RuitnGのコーティングを確認した。PAA-TiOx NPsと比較したRutinG-TiOxの放射線増感効果の検証するために、H2O2の吸脱着量を化学蛍光法によって定量した。PAA-TiOx及びRutinG-TiOx NPsから219.8, 39.5 micro-mol/gのH2O2が脱着した。この結果より、RutinG-TiOxはPAA-TiOxほどではないがH2O2徐放性を持つことが示唆された。 [2] 過酸化チタン/放射線による治療：in vitroでの放射線増感効果を検証するためコロニーアッセイを行った。5Gyを照射した場合、RutinG-TiOx NPsにもPAA-TiOx NPsと同様に放射線増感効果を持つことが示された。


• 血管・リンパ管新生に着眼した歯性感染症－顎骨骨髄炎移行メカニズム解明と治療法探索

  明石 昌也, 木本 明, 長谷川 巧実, 筧 康正

  日本学術振興会, 科学研究費助成事業 基盤研究(C), 基盤研究(C), 神戸大学, 01 Apr. 2019 - 31 Mar. 2023

  骨粗鬆症などに対し用いられる骨吸収抑制薬や、口腔癌に対し施行される放射線照射等の医療行為が素因となる顎骨骨髄炎は、近年患者数の増加に伴い顎口腔外科領域において最も新たな治療法開発が期待される難治性疾患の一つである。本研究の目的は、近年感染の進展にも関与していることが明らかとなりつつある血管・リンパ管新生をターゲットとし、歯性感染症－顎骨骨髄炎移行メカニズムを解明することである。 研究の端緒として、まず血管新生のマーカーとなりうる分子の検討を行なった。近年動脈硬化や高血圧、腫瘍の増大における病的な状態における血管新生に関与している可能性が示唆されている血管内皮細胞間接着複合体構成分子に注目した。この分子についてはマウスやヒト培養細胞を用いた研究はこれまでに行われてきたが、ヒトの組織内での発現や局在部位を確認した報告は稀少であった。当該年度の研究結果より、この分子がヒトの組織内でも特に非炎症部位の血管に比べ炎症部位の微小血管等に発現が強く、病的な血管のマーカーとなり得る可能性が示唆された。


• Development of cancer therapies to improve resistance and reduce side effects

  Hasegawa Takumi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 01 Apr. 2018 - 31 Mar. 2022

  In the present study, the combination of transcutaneous CO2 and radiation therapy significantly inhibited tumor growth compared with other treatments using a local carbon dioxide gas administration method. In the combination group, anti-human ROS modulator 1 and caspase8 were increased by radiation therapy (RT), and mitochondrial apoptosis (PARP, caspase 3, 9) were increased by transcutaneous CO2 therapy. These results indicated that transcutaneous CO2 could potentially improve the antitumor effect of RT by decreasing the intratumoral hypoxia and increasing the mitochondrial apoptosis. Our findings indicated that CO2 therapy may be a novel adjuvant therapy in combination with RT for OSCC.

  Competitive research funding


• Low-level laser therapy: A novel therapy for ARONJ with metabolomic analysis

  Hiroaki Suzuki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 01 Apr. 2017 - 31 Mar. 2021

  For the treatment of anti-resorptive agents-related osteonecrosis of the jaw, Low reactive Level Laser Therapy is indicated overseas than before. In recent years, Low reactive Level Laser Therapy has been recognized as Low reactive Level Light Therapy including light sources such as LEDs in addition to lasers, and this research was started to find out the effectiveness of LED light for anti-resorptive agents-related osteonecrosis. The effect of LED irradiation on the indigenous bacteria in the cultured oral cavity was analyzed. Streptococcus salivarius was irradiated at wavelengths of 405, 630, and 950 nm, and the number of viable bacteria in the LED-irradiated group and the LED-non-irradiated group (control group) was compared, and a decrease in the viable cell count in the LED-irradiated group was observed.


• Myofunction MRI analysis of the oral cancer reconstruction case

  FURUDOI SHUNGO

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Kobe University, 01 Apr. 2017 - 31 Mar. 2020

  Skeletal muscle functional magnetic resonance image (muscle functional Magnetic Resonance Image: mfMRI) using a functional image, the change in T2 value before and after exercise for chewing muscle at rest and chewing in healthy subjects (ΔT2) was measured, ΔT2 increase in bilateral masseter during chewing was confirmed. Morphological standardization of masticatory muscles has become possible. Subsequently, when applying mfMRI to oral cancer reconstruction patients, the health side is maintained the same form and muscle mass as before surgery, chewing force was considered to be maintained. On the other hand, since the resection side is excised by surgery, the morphology is changed muscle mass has been reduced, chewing force has been suggested to be reduced.

  Competitive research funding


• メタボローム解析を用いたARONJに対する新規LED治療の開発

  鈴木 泰明

  学術研究助成基金助成金／基盤研究(C), Apr. 2017 - Mar. 2020

  Competitive research funding


• Analysis of characteristics of lymphatic microcirculation within metastatic lymph nodes focusing on endothelial cell junctional complex

  Akashi Masaya

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Kobe University, 01 Apr. 2015 - 31 Mar. 2019, Principal investigator

  The effects of radiation on morphology, cell integrity, and permeability of lymphatic endothelial cells were analyzed for a better understanding of relationship between cancer and lymphatic vessels. As a result, changes in lymphatic endothelium were found under the influence of radiation, which could contribute to an understanding of the onset mechanism of complications such as lymph node metastasis of cancer and lymphedema after radiation.

  Competitive research funding


• Characterization of junctional complex of lymphatic endothelial cells in caner lymphangiogenesis

  AKASHI Masaya

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Kobe University, 28 Apr. 2011 - 31 Mar. 2015, Principal investigator

  To maintain normal function, the lymphatic endothelium is regulated by cell-cell junctions. One of objectives of this study was to characterize cell junctions in lymphatic endothelial cells and to investigate the effects of the inflammatory cytokine TNF-α on altered cell-cell junctions. Cultured human dermal lymphatic endothelial cells (HDLEC) were immunostained with the tight junction marker, and adherens junction markers, VE-cadherin. In TNF-α-treated HDLEC, we evaluated changes in endothelial cell junctions by immunostaining and through the use of transendothelial electrical resistance (TER). The results revealed a heterogeneous distribution of cell junctions in HDLEC involving continuous and discontinuous junctions. Our data also suggest that TNF-α alters the normal distribution of cell junctions and affects the endothelial barrier of cultured lymphatic endothelial cells.

  Competitive research funding