Research activity information

• DNA repair ability in a patient with voriconazole-related squamous cell carcinoma that required differential diagnosis from xeroderma pigmentosum

  Takeshi Fukumoto, Tomoka Harada, Takamichi Ito, Satoshi Fukushima, Ryusuke Ono, Masutaka Furue, Chikako Nishigori

  Elsevier BV, Feb. 2024, Journal of Dermatological Science

  Scientific journal


• Clinical trial on the efficacy and safety of NPC-15 for patients with xeroderma pigmentosum exaggerated sunburn reaction type: XP-1 study protocol for a multicentre, double-blinded, placebo-controlled, two-group crossover study followed by a long-term open study in Japan.

  Mariko Tsujimoto, Yasumasa Kakei, Nozomi Yamano, Takeshi Fujita, Takehiro Ueda, Ryusuke Ono, Sae Murakami, Shinichi Moriwaki, Chikako Nishigori

  INTRODUCTION: Xeroderma pigmentosum (XP) is a rare intractable disease without a fundamental treatment, presenting with severe photosensitivity, freckle-like pigmented and depigmented maculae and numerous skin cancers before the age of 10 years without strict sun protection. About 70% of the patients exhibit extremely severe sunburn reactions and most of them develop neurological symptoms, including sensorineural hearing impairment and progressive peripheral and central nervous disorders beginning from childhood ages. In the preclinical study, we found that N-acetyl-5-methoxytryptamine was effective in suppressing skin tumour development in addition to improvement of auditory brainstem response in chronically ultraviolet-irradiated XP-A model mice. METHODS AND ANALYSIS: On the bases of the preclinical study, we conduct a clinical trial on the efficacy of NPC-15 for patients with XP with exaggerated sunburn reaction type by a multicentre, double-blinded placebo-controlled, two-group crossover study followed by a 52 weeks open study. ETHICS AND DISSEMINATION: Ethics approval is overseen by the Kobe University Institutional Review Board and Osaka Medical and Pharmaceutical University Institutional Review Board, and the study is conducted in accordance with the approved protocol. All participants will be required to provide written informed consent. Findings will be disseminated through scientific and professional conferences and peer-reviewed journal publications. The data sets generated during the study will be available from the corresponding author on reasonable request. TRIAL REGISTRATION NUMBER: jRCTs051210181.

  Mar. 2023, BMJ open, 13(3) (3), e068112, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Lower efficacy of omalizumab in older adults with chronic spontaneous urticaria.

  Rikuma Kitao, Yoshiko Oda, Ken Washio, Yukimasa Tai, Ryusuke Ono, Chikako Nishigori, Atsushi Fukunaga

  Omalizumab is known to be effective in treating chronic spontaneous urticaria (CSU) with an inadequate response to H1 -antihistamine. Although many reports have described pre-treatment biomarkers to predict the efficacy of omalizumab in CSU, there are few reports that examined the relationship between age and the therapeutic effectiveness of omalizumab. Thus, we aimed to investigate the relationship between response to omalizumab and age. This retrospective study comprised 52 CSU patients receiving three consecutive omalizumab courses during the period from April 2017 to March 2021. Participants were categorized as responders or non/partial responders using the urticaria control test to evaluate clinical variables on week 12. The female rate tended to be higher, and the mean age and the median disease duration tended to be lower with no significance in responders compared with in non/partial responders. In addition, they exhibited no significant differences regarding serum immunoglobulin E levels, basophil counts, eosinophil counts, d-dimer, and autologous serum skin test results reported as predictor in the past between two groups. Interestingly, when patients were categorized as age <65 years or ≥65 years, those in the ≥65 years group had a significantly lower response to omalizumab than those aged <65 years. These findings suggest that physicians should keep in mind that the age of their CSU patients may be a predictor of the therapeutic efficacy of omalizumab.

  Mar. 2022, The Journal of dermatology, 49(7) (7), 729 - 731, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Replication-related genes are upregulated in XP-A cells after UV-C irradiation.

  Seiji Takeuchi, Toshiro Matsuda, Mariko Tsujimoto, Takeshi Fukumoto, Ryusuke Ono, Chikako Nishigori

  BACKGROUND: Xeroderma pigmentosum (XP) is hereditary disorder characterized by photosensitivity, predisposition to skin cancers of sun-exposed body sites and progressive neurologic symptoms in some cases. Cells from XP patients show higher sensitivity to ultraviolet radiation (UV) than normal cells. OBJECTIVE: We aimed to ascertain the genes differentially regulated in XP complementation group A (XP-A) cells after UV irradiation. METHODS: XP-A cells were harvested at 4 or 12 h after a single exposure to low-dose UV-C radiation and subjected to transcriptome analysis by microarray. RESULTS: The number of genes with significantly altered expression (≥2-fold difference) at 12 h was markedly higher in XP-A cells than that in normal cells, suggesting that the number of altered genes could be correlated to the amount of DNA damage. CONCLUSION: We recently reported that mitotic genes are induced in normal human fibroblasts after UV-C exposure, and similar results were observed in XP-A cells as normal cells. In addition, a majority of replication-related genes were significantly upregulated in XP-A cells, whereas no such expression pattern was observed in the normal control cells. Collectively, these results indicate that the XPA protein can transcriptionally inhibit the series of replication-related genes, and could possibly regulate replication and/or re-replication after UV irradiation.

  Mar. 2022, Journal of dermatological science, 105(3) (3), 152 - 158, English, International magazine

  Scientific journal


• 悪性黒色腫肺転移に対してnivolumabにて加療した色素性乾皮症バリアント型の1例

  上田 佳奈, 小野 竜輔, 大郷 真理子, 松本 由佳里, 鷲尾 健, 錦織 千佳子

  (一社)日本皮膚悪性腫瘍学会, Jun. 2021, 日本皮膚悪性腫瘍学会学術大会プログラム・抄録集, 37回, 146 - 146, Japanese


• 慢性蕁麻疹に対するオマリズマブの治療反応性予測因子と長期的使用実態に関する後方視的解析

  北尾 陸将, 織田 好子, 有吉 綾香, 藤井 翔太郎, 田井 志正, 鷲尾 健, 小野 竜輔, 福永 淳, 錦織 千佳子

  (公社)日本皮膚科学会, May 2021, 日本皮膚科学会雑誌, 131(5) (5), 1386 - 1386, Japanese


• Report of two Japanese patients with piebaldism including a novel mutation in KIT.

  Kei Nagatani, Ken Okamura, Kazumoto Katagiri, Ryusuke Ono, Chikako Nishigori, Yuta Araki, Toru Saito, Yutaka Hozumi, Tamio Suzuki

  Feb. 2021, The Journal of dermatology, 48(2) (2), e94-e95, English, International magazine


• Whole-exome sequencing and host cell reactivation assay lead to a diagnosis of xeroderma pigmentosum group D with mild ultraviolet radiation sensitivity.

  Makoto Sugaya, Kaoru Funamizu, Michihiro Kono, Yusuke Okuno, Taisuke Kondo, Ryusuke Ono, Masashi Akiyama, Chikako Nishigori, Shinichi Sato

  A case of xeroderma pigmentosum (XP) group D in a 39-year-old Japanese man is reported. The patient had suffered from moderate to severe solar sensitivity and freckle-like pigmented macules in sun-exposed areas since 6 years of age, and developed skin malignancies such as squamous cell carcinoma, actinic keratosis, Bowen's disease and basal cell carcinoma. The minimal erythema dose for ultraviolet (UV) radiation was decreased with a delayed peak reaction. The level of unscheduled DNA synthesis of fibroblasts from the patient was 70% of normal, while they expressed POLH, a gene product responsible for the XP variant. Whole-exome sequencing indicated that the patient harbored a homozygous mutation of c.1802G>T, p.Arg601Leu in ERCC2. A genetic complementation test was carried out by host cell reactivation assay, which showed that the patient's fibroblasts recovered only when they were transfected with XPD cDNA, confirming the diagnosis of XP-D. Arg601Leu mutation in ERCC2 may be related to mild UV radiation sensitivity and moderate skin lesions.

  Jan. 2021, The Journal of dermatology, 48(1) (1), 96 - 100, English, International magazine

  Scientific journal


• 重症アトピー性皮膚炎長期罹患後に発症したfolliculotropic mycosis fungoidesの1例

  木谷 美湖野, 江崎 諒, 鷲尾 健, 小野 竜輔, 齊藤 泰之, 神保 直江, 錦織 千佳子

  (株)医学書院, May 2020, 臨床皮膚科, 74(6) (6), 423 - 429, Japanese


• 51歳で診断されたXP-G群の1例

  新川 衣里子, 辻本 昌理子, 小野 竜輔, 錦織 千佳子, 徳岡 秀樹, 佐竹 渉, 千原 典夫, 松本 理器, 戸田 達史

  日本皮膚科学会-大阪地方会・京滋地方会, Feb. 2019, 皮膚の科学, 18(1) (1), 53 - 53, Japanese

  [Refereed]


• 【小児先天性皮膚疾患】臨床例 色素性乾皮症D群の小児

  橋本真哉, Ono Ryusuke, 柳原茂人, 錦織千佳子

  Jan. 2019, 皮膚病診療, 41(1号) (1号), 57 - 60, Japanese

  [Refereed][Invited]

  Scientific journal


• 重症アトピー性皮膚炎としてフォローされていたFolliculotropic mycosis fungoidesの1例

  木谷 美湖野, 江崎 諒, 鷲尾 健, 小野 竜輔, 錦織 千佳子, 齊藤 泰之, 神保 直江

  日本皮膚科学会-大阪地方会・京滋地方会, Dec. 2018, 皮膚の科学, 17(6) (6), 375 - 375, Japanese

  [Refereed]


• A case of xeroderma pigmentosum complementation group C with diverse clinical features

  Masaki T, Nakano E, Okamura K, Ono R, Sugasawa K, Lee M-H, Suzuki T, Nishigori C

  Jun. 2018, BRITISH JOURNAL OF DERMATOLOGY, 178(6) (6), 1451 - +, Japanese

  Scientific journal


• Xeroderma Pigmentosum Diagnosis Using a Flow Cytometry-Based Nucleotide Excision Repair Assay.

  Eiji Nakano, Seiji Takeuchi, Ryusuke Ono, Mariko Tsujimoto, Taro Masaki, Chikako Nishigori

  Feb. 2018, The Journal of investigative dermatology, 138(2) (2), 467 - 470, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Co-regulation of Cxcl1 and versican in the inflammatory response to UVB induced reactive oxygen species in skin photo-tumorigenesis.

  Chihiro Takemori, Makoto Kunisada, Flandiana Yogianti, Sugako Oka, Kunihiko Sakumi, Ryusuke Ono, Yusaku Nakabeppu, Chikako Nishigori

  Feb. 2017, Journal of dermatological science, 85(2) (2), 140 - 143, English, International magazine

  [Refereed]


• The present status of xeroderma pigmentosum in Japan and a tentative severity classification scale

  Eiji Nakano, Taro Masaki, Fumio Kanda, Ryusuke Ono, Seiji Takeuchi, Shinichi Moriwaki, Chikako Nishigori

  Aug. 2016, EXPERIMENTAL DERMATOLOGY, 25, 28 - 33, English

  [Refereed]

  Scientific journal


• Mitotic genes are transcriptionally upregulated in the fibroblast irradiated with very low doses of UV-C

  Seiji Takeuchi, Toshiro Matsuda, Ryusuke Ono, Mariko Tsujimoto, Chikako Nishigori

  Jul. 2016, SCIENTIFIC REPORTS, 6, 29233, English

  [Refereed]

  Scientific journal


• A 10-year follow-up of a child with mild case of xeroderma pigmentosum complementation group D diagnosed by whole-genome sequencing.

  Ryusuke Ono, Taro Masaki, Franklin Mayca Pozo, Yuka Nakazawa, Sigrid M A Swagemakers, Eiji Nakano, Wataru Sakai, Seiji Takeuchi, Fumio Kanda, Tomoo Ogi, Peter J van der Spek, Kaoru Sugasawa, Chikako Nishigori

  BACKGROUND: Most patients with xeroderma pigmentosum complementation group D (XP-D) from Western countries suffer from neurological symptoms, whereas Japanese patients display only skin manifestations without neurological symptoms. We have previously suggested that these differences in clinical manifestations in XP-D patients are attributed partly to a predominant mutation in ERCC2, and the allele frequency of S541R is highest in Japan. METHODS: We diagnosed a child with mild case of XP-D by the evaluation of DNA repair activity and whole-genome sequencing, and followed her ten years. RESULTS: Skin cancer, mental retardation, and neurological symptoms were not observed. Her minimal erythema dose was 41 mJ/cm(2) , which was slightly lower than that of healthy Japanese volunteers. The patient's cells showed sixfold hypersensitivity to UV in comparison with normal cells. Post-UV unscheduled DNA synthesis was 20.4%, and post-UV recovery of RNA synthesis was 58% of non-irradiated samples, which was lower than that of normal fibroblasts. Genome sequence analysis indicated that the patient harbored a compound heterozygous mutation of c.1621A>C and c.591_594del, resulting in p.S541R and p.Y197* in ERCC2: then, patient was diagnosed with XP-D. Y197* has not been described before. CONCLUSION: Her mild skin manifestations might be attributed to the mutational site on her genome and daily strict sun protection. c.1621A>C might be a founder mutation of ERCC2 among Japanese XP-D patients, as it was identified most frequently in Japanese XP-D patients and it has not been found elsewhere outside Japan.

  Jul. 2016, Photodermatology, photoimmunology & photomedicine, 32(4) (4), 174 - 80, English, International magazine

  [Refereed]

  Scientific journal


• 免疫抑制剤の長期使用中に発生した皮膚悪性腫瘍の2例

  小猿恒志, 津村協子, 仲田かおり, Ono Ryusuke, 岡昌宏, 永井宏, 錦織千佳子

  Apr. 2015, 皮膚の科学, 14(2号) (2号), 89 - 90, Japanese

  [Refereed]

  Scientific journal


• Inhibitory Effects of Dietary Spirulina platensis on UVB-Induced Skin Inflammatory Responses and Carcinogenesis

  Flandiana Yogianti, Makoto Kunisada, Eiji Nakano, Ryusuke Ono, Kunihiko Sakumi, Sugako Oka, Yusaku Nakabeppu, Chikako Nishigori

  Oct. 2014, JOURNAL OF INVESTIGATIVE DERMATOLOGY, 134(10) (10), 2610 - 2619, English

  [Refereed]

  Scientific journal


• Differences in Clinical Phenotype among Patients with XP Complementation Group D: 3D Structure and ATP-Docking of XPD In Silico

  Eiji Nakano, Ryusuke Ono, Taro Masaki, Seiji Takeuchi, Yutaka Takaoka, Eiichi Maeda, Chikako Nishigori

  Jun. 2014, JOURNAL OF INVESTIGATIVE DERMATOLOGY, 134(6) (6), 1775 - 1778, English

  [Refereed]

  Scientific journal


• Suppressive effect of administration of recombinant human thioredoxin on cutaneous inflammation caused by UV

  Ryusuke Ono, Atsushi Fukunaga, Taro Masaki, Xijun Yu, Junji Yodoi, Chikako Nishigori

  Jul. 2013, Bioengineered, 4(4) (4), 254 - 257, English

  [Refereed]

  Scientific journal


• Suppressive effect of administration of recombinant human thioredoxin on cutaneous inflammation caused by UV

  Ryusuke Ono, Atsushi Fukunaga, Taro Masaki, Xijun Yu, Junji Yodoi, Chikako Nishigori

  Jul. 2013, BIOENGINEERED, 4(4) (4), 254 - 257, English

  [Refereed]

  Scientific journal


• 皮膚有棘細胞癌と鑑別を要した卵巣成熟嚢胞奇形腫によるSister Mary Joseph's Noduleの1例

  濱岡 大, 尾藤 利憲, 坂口 正展, 池田 哲哉, 小野 竜輔, 中林 幸士, Nishigori Chikako

  A 68-year-old woman was referred to our hospital in October 2011 for an umbilical nodule that had appeared 2 months earlier. A skin biopsy specimen taken from the lesion histologically showed atypical squamous cell invasion from the epidermis into the dermis, suggesting cutaneous squamous cell carcinoma. Magnetic resonance imaging (MRI) was performed before treatment, and revealed that she had a smooth tumor, approximately 10.5×13.5×17 centimeters at her right ovary. At that time, we diagnosed her as having a cutaneous squamous cell carcinoma and a benign ovarian tumor. However, during the operation, the ovarian tumor was diagnosed as being histologically malignant. Thus, the patient underwent wide-excision. Finally, we diagnosed this case as being a Sister Mary Joseph's nodule associated with mature cystic ovarian teratoma. She underwent additional chemotherapy combined with Paclitaxel and Carboplatin. During the follow up over the 18 subsequent months, there was no evidence of disease recurrence or metastasis. Although malignant transformation of a mature cystic ovarian teratoma is very rare, in such a case the tumor occasionally presents as a squamous cell carcinoma. Thus, a Sister Mary Joseph's nodule originating from mature cystic ovarian teratoma is indistinguishable from cutaneous squamous cell carcinoma. This article reports important information for dermatologists.[Skin Cancer (Japan) 2013 ; 28 : 34-38]

  The Japanese Skin Cancer Society, Jun. 2013, Skin Cancer, 28(1号) (1号), 34 - 38, Japanese

  [Refereed]

  Scientific journal


• Three school-age cases of xeroderma pigmentosum variant type

  Ryusuke Ono, Taro Masaki, Seiji Takeuchi, Ayako Shimizu, Miki Tanioka, Naotomo Kambe, Hiroyuki Matsue, Ryoichi Kamide, Chikako Nishigori

  Jun. 2013, PHOTODERMATOLOGY PHOTOIMMUNOLOGY & PHOTOMEDICINE, 29(3) (3), 132 - 139, English

  [Refereed]

  Scientific journal


• Hydrochlorothiazide enhances UVA-induced DNA damage

  Makoto Kunisada, Taro Masaki, Ryusuke Ono, Hironobu Morinaga, Eiji Nakano, Flandiana Yogianti, Kunihiro Okunishi, Hiroshi Sugiyama, Chikako Nishigori

  May 2013, Photochemistry and Photobiology, 89(3) (3), 649 - 654, English

  [Refereed]

  Scientific journal


• 腸上皮化生を認めたストマ周囲潰瘍の1例

  小野 竜輔, 坂口 正展, 国定 充, 長野 徹, 岡 昌宏, 錦織 千佳子, 川上 史

  (公社)日本皮膚科学会, Apr. 2013, 日本皮膚科学会雑誌, 123(4) (4), 494 - 494, Japanese

  [Refereed]


• Thioredoxin ameliorates cutaneous inflammation by regulating the epithelial production and release of pro-Inflammatory cytokines

  Hai Tian, Yoshiyuki Matsuo, Atsushi Fukunaga, Ryusuke Ono, Chikako Nishigori, Junji Yodoi

  2013, FRONTIERS IN IMMUNOLOGY, 4, English

  [Refereed]

  Scientific journal


• Syringomatous carcinoma on the leg

  Ken Washio, Toshinori Bito, Ryusuke Ono, Tatsuya Horikawa, Chikako Nishigori

  Dec. 2012, JOURNAL OF DERMATOLOGY, 39(12) (12), 1041 - 1043, English

  [Refereed]

  Scientific journal


• 【顔面の腫瘤 鑑別診断と治療】 色素性乾皮症に合併する顔面の皮膚腫瘍

  Ono Ryusuke, Nishigori Chikako

  Dec. 2012, Derma., (199号) (199号), 1 - 6, Japanese

  [Refereed]

  Scientific journal


• Skin tumours induced by narrowband UVB have higher frequency of p53 mutations than tumours induced by broadband UVB independent of Ogg1 genotype.

  Yogianti F, Kunisada M, Ono R, Sakumi K, Nakabeppu Y, Nishigori C

  Different wavelengths of ultraviolet (UV) light have different promoting effects on skin carcinogenesis. Narrowband UVB (NB-UVB) has a single-peak wavelength of 311 nm and is widely used for treating skin diseases. Our previous work showed that, in comparison with conventional broadband UVB (BB-UVB), long-term exposure to NB-UVB induces higher frequency of skin cancer in mice, and it suggested that this is mediated through the formation of cyclobutane pyrimidine dimers (CPDs). To explore whether the frequency of p53 mutations in skin tumours correlates with CPD-induced mutations, we compared the frequency and types of p53 mutations between NB-UVB-induced and BB-UVB-induced malignant skin tumours produced in wild-type and Ogg1 knockout mice, which are deficient in repair of oxidative 8-oxoguanine (8-oxoG), a DNA damage mediated by reactive oxygen species (ROS). The frequency of p53 mutation was significantly higher in NB-UVB-induced than in BB-UVB-induced tumours in both wild-type and Ogg1 knockout mice. Most of the p53 mutations found were G:C → A:T transitions at dipyrimidine sites in both the NB-UVB- and BB-UVB-exposed groups. However, G:C → T:A mutations caused by 8-oxoG did not increase in Ogg1 knockout mice exposed to either NB-UVB or BB-UVB. Our results strongly suggest that NB-UVB induces highly malignant tumours caused by p53 dipyrimidine mutations through the formation of CPDs.

  Nov. 2012, Mutagenesis, 27(6) (6), 637 - 643, English, International magazine

  [Refereed]

  Scientific journal


• Suppressive effect of recombinant human thioredoxin on ultraviolet light-induced inflammation and apoptosis in murine skin

  Ryusuke Ono, Taro Masaki, Siphora Dien, Xijun Yu, Atsushi Fukunaga, Junji Yodoi, Chikako Nishigori

  Oct. 2012, JOURNAL OF DERMATOLOGY, 39(10) (10), 843 - 851, English

  [Refereed]

  Scientific journal


• レボセチリジン塩酸塩の皮脂欠乏性皮膚炎に対する有用性の検討

  Fukunaga Atsushi, Kanki Haruhisa, Ono Ryusuke, Nishigori Chikako

  Sep. 2012, 診療と新薬, 49(9号) (9号), 1131 - 1137, Japanese

  [Refereed]

  Scientific journal


• Six cases of photosensitive disorders probably due to hydrochlorothiazide

  Eriko Tanaka, Toshinori Bito, Eriko Masuoka, Ryusuke Ono, Hideki Shimizu, Youzou Yamada, Masahiro Oka, Chikako Nishigori, Shimano Ikemura

  Apr. 2011, Skin Research, 10(2) (2), 133 - 140, Japanese

  Scientific journal


• Agents that Reverse UV-Induced Immune Suppression and Photocarcinogenesis Affect DNA Repair

  Sreevidya CS, Fukunaga Atsushi, Khaskhely NM, Masaki T, Ono R, Nishigori Chikako, Ullrich SE

  Oct. 2009, J Invest Dermatol, 130(5) (5), 1428 - 1437, English

  [Refereed]

  Scientific journal


• Four types of possible founder mutations are responsible for 87% of Japanese patients with Xeroderma pigmentosum variant type.

  Masaki Taro, Ono Ryusuke, Tanioka Miki, Funasaka Yoko, Nagano Tohru, Moriwaki Shinichi, Nishigori Chikako

  Nov. 2008, Journal of dermatological science, 52(2) (2), 144 - 8, English


• 重複癌に合併した線状IgA水疱性皮膚症

  川崎 加織, 山本 微香, 津田 達也, 夏秋 優, Ono Ryusuke, 石井 文人, 橋本 隆, 山西 清文

  医学書院, May 2008, 臨床皮膚科, 62(6号) (6号), 390 - 393, Japanese

  [Refereed]

  Scientific journal


• 【薬疹-2005】 臨床例 メシル酸イマチニブによる扁平苔癬型薬疹

  Ono Ryusuke, 村田 洋三, 熊野 公子

  Oct. 2005, 皮膚病診療, 27(10号) (10号), 1185 - 1188, Japanese

  [Refereed]

  Scientific journal


• 重症皮膚石灰沈着症を伴ったoverlap症候群の1例

  正木 太朗, 大橋 明子, 山本 麻由, 岩尾 麻依子, Ono Ryusuke, 田中 美奈子, 藤原 規広, 市橋 正光, 田村 真吾

  医学書院, May 2003, 臨床皮膚科, 57(6号) (6号), 473 - 475, Japanese

  [Refereed]

  Scientific journal

• 当施設にてSPECT/CTを利用したRI法によりセンチネルリンパ節生検を検討した皮膚悪性腫瘍72症例のまとめ

  橋本 真哉, 神保 晴紀, 新川 衣里子, 中野 英司, 藤原 進, 小野 竜輔, 錦織 千佳子

  (一社)日本皮膚悪性腫瘍学会, Dec. 2020, 日本皮膚悪性腫瘍学会学術大会プログラム・抄録集, 36回, 161 - 161, Japanese


• 尋常性天疱瘡の治療中に足趾に生じた増殖性天疱瘡の1例

  小野 竜輔, 横山 大輔, 竹内 千尋, 吉岡 愛育, 中野 英司, 錦織 千佳子

  日本皮膚科学会-大阪地方会・京滋地方会, Dec. 2020, 皮膚の科学, 19(4) (4), 269 - 270, Japanese


• 巨大な有棘細胞癌を形成したXP-C群の1例

  樫本 梨沙, 小野 竜輔, 飯田 裕里佳, 新川 衣里子, 田井 志正, 中野 英司, 錦織 千佳子

  (公社)日本皮膚科学会, May 2020, 日本皮膚科学会雑誌, 130(5) (5), 1235 - 1235, Japanese


• Characteristics of Xeroderma Pigmentosum in Japan: Lessons From Two Clinical Surveys and Measures for Patient Care

  NISHIGORI CHIKAKO, Nakano E, Masaki T, Ono Ryusuke, Takeuchi S, Tsujimoto M, Ueda Takehiro

  Xeroderma pigmentosum (XP) is a rare autosomal recessive hereditary disease caused by deficiency in repair of DNA lesions generated by ultraviolet radiation and other compounds. Patients with XP display pigmentary change and numerous skin cancers in sun-exposed sites, and some patients show exaggerated severe sunburns even upon minimum sun exposure as well as neurological symptoms. We conducted a nationwide survey for XP since 1980. In Japan, the frequency of the XP complementation group A is the highest, followed by the variant type; while in the Western countries, those of groups C or D are the highest. Regarding skin cancers in XP, basal cell carcinoma was the most frequent cancer that afflicted patients with XP, followed by squamous cell carcinoma, and malignant melanoma. The frequency of these skin cancers in patients with XP has decreased in these 20 years, and the age of onset of developing skin cancers is higher than those previously observed, owing to early diagnosis and education to patients and care takers on strict prevention from sunlight for patients with XP. On the other hand, the effective therapy for neurological XP has not been established yet, and this needs to be done urgently.

  Jan. 2019, Photochem Photobiol, 95(1) (1), 140 - 153, English, International magazine

  [Refereed]

  Introduction scientific journal


• Truncated XPA protein could not interact with TFIIH but presented mild clinical manifestations

  E. Nakano, M. Tusjimoto, S. Takeuchi, R. Ono, T. Masaki, K. Sugasawa, C. Nishigori

  May 2017, JOURNAL OF INVESTIGATIVE DERMATOLOGY, 137(5) (5), S131 - S131, English

  Summary international conference


• GENOTYPE -PHENOTYPE CORRELATION AMONG XERODERMA PIGMENTOSUM COMPLEMENTATION GROUP D

  Eiji Nakano, Ryusuke Ono, Taro Masaki, Seiji Takeuchi, Yutaka Takaoka, Kaoru Sugasawa, Chikako Nishigori

  Oct. 2014, JOURNAL OF DERMATOLOGY, 41, 14 - 14, English

  Summary international conference


• 低線量紫外線照射が遺伝子発現プロファイルに与える影響

  松田 外志朗, 竹内 聖二, 小野 竜輔, 錦織 千佳子

  (一社)日本放射線影響学会, Sep. 2014, 日本放射線影響学会大会講演要旨集, 57回, 143 - 143, Japanese


• A群色素性乾皮症患者細胞における低線量紫外線照射時の網羅的遺伝子発現解析

  竹内 聖二, 松田 外志朗, 小野 竜輔, 錦織 千佳子

  (一社)日本放射線影響学会, Sep. 2014, 日本放射線影響学会大会講演要旨集, 57回, 144 - 144, Japanese


• Hearing loss in Xeroderma pigmnetosum and mechanism of inner ear disorder.

  Yamashita Daisuke, Fujita Takeshi, Masaki Taro, Takeuchi Seiji, Ueda Takehiro, Sakai Yoshitada, Nakagawa Taku, Toyoshima Daisaku, Morisada Naoya, Kanda Fumio, Nishigori Chikako

  Mar. 2014, International Symposium on Xeroderma Pigmentosum and Related Disease, English

  Summary national conference


• The inhibitory effect of Spirulina platensis on UVB-induced skin carcinogenesis: Anti-inflammatory and antioxidant mechanisms

  F. Yogianti, M. Kunisada, R. Ono, K. Sakumi, Y. Nakabeppu, C. Nishigori

  May 2013, JOURNAL OF INVESTIGATIVE DERMATOLOGY, 133, S214 - S214, English

  Summary international conference


• Suppressive effect of administration of human recombinant thioredoxin on ultrviolet light-induced inflammation and apoptosis of murine skin

  Ono Ryusuke, Fukunaga Atsushi, Masaki Taro, Yu X, Yodoi J, Nishigori Chikako

  Jan. 2013, Bioengineered, 4(4) (4), 1 - 4, English

  Introduction other


• Peristomal skin ulcer with intestinal metaplasia

  R. Ono, M. Oka, M. Sakaguchi, F. Kawakami, T. Nagano, M. Kunisada, C. Nishigori

  Jul. 2012, BRITISH JOURNAL OF DERMATOLOGY, 167(1) (1), 204 - 206, English

  Report scientific journal


• Increased expression of versican in the inflammatory response to UVB- and reactive oxygen species-induced skin tumorigenesis.

  Makoto Kunisada, Flandiana Yogianti, Kunihiko Sakumi, Ryusuke Ono, Yusaku Nakabeppu, Chikako Nishigori

  Excessive exposure to UV radiation is a major risk factor for developing skin cancer. UV-induced reactive oxygen species (ROS) cause accumulation of DNA damage products such as 8-oxoguanine (8-oxoG) in the skin. We have previously shown that mice lacking the repair enzyme 8-oxoguanine glycosylase (Ogg1 knockout mice) are highly susceptible to skin cancer after long-term UVB exposure. To investigate the genes involved, we performed gene profiling of Ogg1 knockout mouse skin after UVB exposure. Among the up-regulated genes in UVB-treated Ogg1 knockout mice, inflammatory response pathway-related genes were most affected. The Vcan gene, which encodes the large extracellular matrix proteoglycan versican, was continuously up-regulated in UVB-treated Ogg1 knockout mice, suggesting that versican is a mediator of skin cancer development. We examined the expression pattern of versican in skin tumors from wild-type mice and UVB-treated Ogg1 knockout mice, and also analyzed 157 sun-related human skin tumors. Versican was strongly expressed in malignant skin tumors in both mice and humans, and especially in Ogg1 knockout mice. Additionally, infiltrating neutrophils strongly colocalized with versican in UVB-treated Ogg1 knockout mouse skin. These data demonstrate that inflammatory responses, particularly neutrophil infiltration and versican up-regulation, are closely involved in UVB/ROS-induced skin tumorigenesis.

  Dec. 2011, The American journal of pathology, 179(6) (6), 3056 - 65, English, International magazine


• Founder mutations in the DNA polymerase eta gene in Japanese patients diagnosed as xeroderma pigmentosum variant type

  T. Masaki, R. Ono, M. Tanioka, T. Nagano, Y. Funasaka, S. Moriwaki, C. Nishigori

  Apr. 2008, JOURNAL OF INVESTIGATIVE DERMATOLOGY, 128, S123 - S123, English

  Summary international conference


• Molecular analysis of DNA polymerase eta gene in Japanese patients diagnosed as xeroderma pigmentosum variant type

  Miki Tanioka, Taro Masaki, Ryusuke Ono, Tohru Nagano, Eriko Otoshi-Honda, Yasuhiro Matsumura, Masahiro Takigawa, Hiroki Inui, Yoshiki Miyachi, Shinichi Moriwaki, Chikako Nishigori

  Jul. 2007, JOURNAL OF INVESTIGATIVE DERMATOLOGY, 127(7) (7), 1745 - 1751, English

  Introduction scientific journal


• A case of aspirin intolerance with anisakis-induced urticaria and positive intradermal test with autologous serum

  小野 竜輔, 奥窪 美佳, 福永 淳

  医学書院, Oct. 2004, 臨床皮膚科, 58(11) (11), 920 - 923, Japanese

• 日経メディクイズ 皮膚 鑑別診断の基本 / [case08]49歳、男性。顔面の色素半の増加

  Ono Ryusuke, Nishigori Chikako

  Others, 日経BP社, Nov. 2012, Japanese

  General book

• 創内持続陰圧洗浄療法により治療したフルニエ壊疽の1例

  中村 彩, 鷲見 真由子, 新川 衣里子, ONO RYUSUKE, NISHIGORI CHIKAKO

  第471回日本皮膚科学会大阪地方会, Feb. 2019, Japanese, 大阪, Domestic conference

  Oral presentation


• びまん性神経線維腫症に動脈塞栓療法を併用して治療したNF1の1例

  ONO RYUSUKE, NISHIGORI CHIKAKO

  第10回レックリングハウゼン病学会学術大会, Feb. 2019, Japanese, 名古屋, Domestic conference

  Oral presentation


• 51歳で診断されたXP-G群の1例

  新川 衣里子, TSUJIMOTO MARIKO, ONO RYUSUKE, NISHIGORI CHIKAKO, 徳岡 秀樹, 千原 典夫, 佐竹 渉

  第471回日本皮膚科学会大阪地方会, Feb. 2019, Japanese, 大阪, Domestic conference

  Oral presentation


• 重症アトピー性皮膚炎としてフォローされていたFolliculotropic mycosis fungoidesの1例

  木谷 美湖野, 江崎 諒, WASHIO KEN, ONO RYUSUKE, NISHIGORI CHIKAKO, 齊藤 泰之, JIMBO NAOE

  第470回 日本皮膚科学会 大阪地方会, Dec. 2018, Japanese, 大阪, Domestic conference

  Oral presentation


• 神経症状を合併した色素性乾皮症D群の2例

  ONO RYUSUKE, NAKANO EIJI, 上原 奈津美, 上田 健博, 酒井 良忠, 栁原 茂人, NISHIGORI CHIKAKO

  第40回日本光医学・光生物学会, Jul. 2018, Japanese, 仙台, Domestic conference

  Oral presentation


• 色素性乾皮症D群の小児の1例

  橋本 真哉, NAKANO EIJI, ONO RYUSUKE, MASAKI TARO, TAKEUCHI SEIJI, 柳原 茂人, NISHIGORI CHIKAKO

  第42回日本小児皮膚科学会学術大会, Jul. 2018, Japanese, 東京, Domestic conference

  Oral presentation


• ペムブロリズマブ投与後に苔癬様反応を示す異なった形態の皮疹をきたした１例

  江﨑 諒, NISHIYAMA SATOSHI, 橋本 真哉, 中谷 祥子, 中内 恵美, ONO RYUSUKE, NISHIGORI CHIKAKO

  第111回近畿集談会 第468回大阪地方会・第456回京滋地方会, Jul. 2018, Japanese, 京都, Domestic conference

  Oral presentation


• ペムブロリズマブ投与後に苔癬様反応を示す異なった形態の皮疹をきたした１例

  江﨑 諒, NISHIYAMA SATOSHI, 橋本 真哉, 中谷 祥子, 中内 恵美, ONO RYUSUKE, NISHIGORI CHIKAKO

  第120回兵庫県皮膚科医会, Jun. 2018, Japanese, 神戸, Domestic conference

  Oral presentation


• 結節性紅斑を合併した肉芽腫性乳腺炎の１例

  桑原 咲, 大幡万里恵, 福本絢子, ONO RYUSUKE, NISHIGORI CHIKAKO, 谷野裕一

  第467回日本皮膚科学会大阪地方会, May 2018, Japanese, 大阪, Domestic conference

  Oral presentation


• The present status of Xeroderma pigmentosum in Japan-evaluation of symptoms by severity scale score.

  Nishigori Chikako, Nakano Eiji, Kunisada Makoto, Ueda Takehiro, Tsujimoto Mariko, Fujita T, Ono Ryusuke

  国際シンポジウム『早老症と関連疾患2018』（International Meeting on RECQ Helicases and Related Diseases 2018）, Feb. 2018, English, 難病医学研究財団, 千葉, International conference

  [Invited]

  Nominated symposium


• Replication-related genes are upregulated in XP-A cells after UV-C irradiation.

  Seiji Takeuchi, Toshiro Matsuda, Ono Ryusuke, Tsujimoto Mariko, Nishigori Chikako

  日本研究皮膚科学会第42回年次学術大会・総会, Dec. 2017, English, 日本研究皮膚科学会, 高知, Domestic conference

  Oral presentation


• Truncated XPA protein could not interact with TFIIH but presented mild clinical manifestations.

  Nakano Eiji, Tsujimoto Mariko, Seiji Takeuchi, Ono Ryusuke, Taro Masaki, Kaoru Sugasawa, Nishigori Chikako

  2017 SID Annual Meeting, Apr. 2017, English, Society for Investigative Dermatology, Portland, OR, USA, International conference

  Poster presentation


• Usefulness of flow-cytometry based nucleotide excision repair assay for diagnosis of xeroderma pigmentosum variant type

  Nakano Eiji, Seiji Takeuchi, Masaki Taro, Ono Ryusuke, Nishigori Chikako

  日本研究皮膚科学会第40回年次学術大会・総会, Dec. 2015, English, 日本研究皮膚科学会, 岡山, Domestic conference

  Oral presentation


• 異常な日光皮膚炎症状を伴わない小児の色素性乾皮症の2例

  Nakano Eiji, Masaki Taro, Ono Ryusuke, Kasagi Shinpei, 中町 祐司, Kawano Seiji, 深井 和吉, 新関 寛徳, Nishigori Chikako

  第39回日本小児皮膚科学会学術大会, Jul. 2015, Japanese, 日本小児皮膚科学会, 鹿児島, Domestic conference

  Oral presentation


• Transcriptome analysis with microarray in the human fibroblast exposed by low dose of UV

  Seiji Takeuchi, Toshiro Matsuda, Ono Ryusuke, Nishigori Chikako

  15th International Congress of Radiation Research, May 2015, English, International Congress of Radiation Research, Kyoto, International conference

  Poster presentation


• 生体腎移植後の免疫抑制剤長期内服中に発症した露光部皮膚悪性腫瘍の2例

  小猿 恒志, 津村 協子, 仲田 かおり, Ono Ryusuke, Oka Masahiro, Nishigori Chikako

  第447回日本皮膚科学会大阪地方会, Feb. 2015, Japanese, 日本皮膚科学大阪地方会, 大阪, Domestic conference

  Oral presentation


• NER assay based on flow cytometery of pyrimidine dimerimmunocytochemistry: comparison with unscheduled DNA synthesis using autoradiography

  Nakano Eiji, Seiji Takeuchi, Ono Ryusuke, Masaki Taro, Nishigori Chikako

  日本研究皮膚科学会 第39回年次学術大会・総会, Dec. 2014, English, 日本研究皮膚科学会, 大阪, Domestic conference

  Oral presentation


• 当科で経験した日光蕁麻疹22例の検討

  仲田 かおり, 田島 翔子, Ono Ryusuke, Fukunaga Atsushi, Nishigori Chikako

  第44回日本皮膚アレルギー・接触皮膚炎学会総会学術大会, Nov. 2014, Japanese, 日本皮膚アレルギー・接触皮膚炎学会, 仙台, Domestic conference

  Oral presentation


• 低線量紫外線照射が遺伝子発現プロファイルに与える影響

  松田 外志朗, 竹内 聖二, Ono Ryusuke, Nishigori Chikako

  第57回日本放射線影響学会, Oct. 2014, Japanese, 日本放射線影響学会, 鹿児島, Domestic conference

  Oral presentation


• A群色素性乾皮症患者細胞における低線量紫外線照射時の網羅的遺伝子発現解析

  竹内 聖二, 松田 外志朗, Ono Ryusuke, Nishigori Chikako

  第57回日本放射線影響学会, Oct. 2014, Japanese, 日本放射線影響学会, 鹿児島, Domestic conference

  Oral presentation


• Genotype -Phenotype Correlation Among Xeroderma Pigmentosum Complementation Group D.

  Nakano Eiji, Ono Ryusuke, Masaki Taro, Seiji Takeuchi, Takaoka Yutaka, Sugasawa Kaoru, Nishigori Chikako

  3rd Eastern Asia Dermatology Congress, Sep. 2014, English, Eastern Asia Dermatology Congress, Jeju, Korea, 韓国, International conference

  Public symposium


• Molecular analysis of DNA repair defects in cells from Japanese patients with xeroderma pigmentosum Group D

  Mayca Pozo, F, Sakai,W, van der Spek, P. J, Nakazawa, Y, Ogi, T, Ono, R, Nishigori, C, Sugasawa,K

  International Symposium on Xeroderma Pigmentosum and Related Diseases, Mar. 2014, English, Kobe, Japan, International conference

  Poster presentation


• DNA修復異常を伴う光線過敏症患者の分子細胞生物学的解析

  酒井 恒, Franklin Mayca Pozo, 菅澤 薫, 中沢 由華, 荻 朋男, 小野 竜輔, 錦織 千佳子

  厚生労働省科学研究費「神経皮膚症候群に関する調査研究班」平成25年度総会, Dec. 2013, Japanese, 神経皮膚症候群に関する調査研究班, 東京, Domestic conference

  Others


• 塩酸ジブカイン含有OTC外用薬による接触皮膚炎の2例

  Ono Ryusuke, Nishigori Chikako

  UV-ABClub 39, Mar. 2010, Japanese, UV-ABClub, 京都, Domestic conference

  Oral presentation


• 妊娠にて増悪した連圏状粃糠疹の1例

  Ono Ryusuke, Fukunaga Atsushi, Nishigori Chikako, Kanki Haruhisa

  日本皮膚科学会第415回大阪地方会, Oct. 2009, Japanese, 大阪地方会, 大阪, Domestic conference

  Oral presentation


• 色素性乾皮症バリアント群の小児の２例

  Ono Ryusuke, Nishigori Chikako

  第31回日本光医学・光生物学会, Jul. 2009, Japanese, 日本光医学・光生物学会, 大阪, Domestic conference

  Oral presentation


• Photoallergic contact dermatitis due to OTC topical medicament containing dibcaine hydrochloride.

  Ono Ryusuke, Nishigori Chikako

  The 4th Joint Meeting of JDA and ACD, Jul. 2009, English, Japanese Dermatological Association, Australasian College of Dermatologists, 札幌, International conference

  Public symposium


• ジブカイン含有OTC皮膚外用薬による光接触性皮膚炎

  Ono Ryusuke, Nishigori Chikako

  第85回兵庫県皮膚科医会総会・学術集談会, Jun. 2009, Japanese, 兵庫県皮膚科医会, 神戸, Domestic conference

  Oral presentation


• 神経症状を伴わない色素性乾皮症Ｄ群の5例

  Ono Ryusuke, Nishigori Chikako

  UV-ABClub 38, Feb. 2009, Japanese, UV-ABClub, 名古屋, Domestic conference

  Oral presentation


• 日本人色素性乾皮症バリアント群における遺伝子変異

  Ono Ryusuke, Nagano Toru, Funasaka Yoko, Nishigori Chikako

  第30回日本光医学・光生物学会, Jul. 2008, Japanese, 日本光医学・光生物学会, 松江, Domestic conference

  Invited oral presentation


• Founder mutations in the DNA polymerase eta gene in Japanese patients diagnosed as xeroderma pigmentosum variant type.

  Ono Ryusuke, Funasaka Yoko, Nishigori Chikako

  International Investigative Dermatology 2008, May 2008, English, IID, 京都, International conference

  Poster presentation


• 色素性乾皮症C群の小児例

  Ono Ryusuke, Nagano Toru, Nishigori Chikako

  UV-ABClub37, Mar. 2008, Japanese, UV-ABClub, 大阪, Domestic conference

  Oral presentation


• 色素性乾皮症C群の小児例

  Ono Ryusuke, Nagano Toru, Nishigori Chikako

  第405回日本皮膚科学会大阪地方会, Feb. 2008, Japanese, 日本皮膚科学会大阪地方会, 大阪, Domestic conference

  Oral presentation

• 多発血管腫に着目した皮膚血管内皮「セカンドヒット病」の探索と新規原因遺伝子の同定

  小野 竜輔, 久保 亜紀子, 吉岡 愛育, 福本 毅

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2024 - 31 Mar. 2027