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MANNEN HideyukiGraduate School of Agricultural Science / Department of Bioresource ScienceProfessor
Researcher basic information
■ Research Keyword■ Research Areas
■ Committee History
- Apr. 2024 - Present, 大学基準協会, 評価委員
- Aug. 2020 - Present, JST, 創発的研究支援事業アドバイザー
- Apr. 2018 - Present, 学術システム研究センター, 研究員
- Apr. 2018 - Present, 国研）水産研究・教育機構, ブリ優良人工種苗推進委員会評価委員
- Apr. 2018 - Present, 東北大学農学部等, 評価委員「豚の抗病性向上手法開発事業」
- Apr. 2013 - Present, 日本畜産学会, 編集委員
- Oct. 2012 - Present, 在来家畜研究会, 会長
- 2012 - Present, 農林水産消費安全技術センター, ISO/TC34/SC16国内対策委員
- Oct. 2008 - Present, 在来家畜研究会, 庶務幹事
- Apr. 2008 - Present, 兵庫県畜産共進会, 顧問
- Oct. 2002 - Present, 日本動物遺伝育種学会, 理事
- Mar. 2020 - Oct. 2020, 関西畜産学会, 関西畜産学会賞受賞候補者選考委員会委員
- Apr. 2019 - Mar. 2020, 日本畜産学会, 日本畜産学会賞・奨励賞受賞候補者選考委員会委員
- Oct. 2011 - Mar. 2020, 関西畜産学会, 評議員
- Apr. 2005 - Mar. 2017, 家畜改良センター, 調査研究評価委員
- Oct. 2012 - Sep. 2016, 日本動物遺伝育種学会, 会長
Research activity information
■ Award- Nov. 2024 日本動物遺伝育種学会, 優秀発表賞・学会長特別賞, ウシ6番染色体の脂肪酸組成QTLに対する責任多型の探索
- Nov. 2023 日本動物遺伝育種学会, 優秀発表賞・学会長特別賞, ミトゲノム解析によるアジア在来ヤギハプログループBの起源および伝播経路の推定
- Nov. 2023 日本動物遺伝育種学会, 優秀発表賞・学会長特別賞, 兵庫県黒毛和種集団を用いた17番染色体における脂肪交雑QTLの探索
- Nov. 2023 日本動物遺伝育種学会, 優秀発表賞, 全ゲノムリシーケンスデータを用いたヤギ高地適応遺伝子多型の探索
- Sep. 2023 日本畜産学会, 優秀発表賞, 宮崎県黒毛和種集団を用いたウシ7番染色体における脂肪交雑に対する原因多型の探索
- Sep. 2023 日本畜産学会, 優秀発表賞, 高密度 SNPマーカーを用いた東南アジア諸島在来ヤギの遺伝構造と伝播、遺伝子流入の推定
- Nov. 2021 日本動物遺伝育種学会, 第22回日本動物遺伝育種学会大会優秀発表賞, アジ ア在来ヤギにおける角の有無と既報の 1番染色体無角関連多型との関連
- Nov. 2021 日本動物遺伝育種学会, 第22回日本動物遺伝育種学会大会優秀発表賞学会長特別賞, 父母系および核ゲノムSNPマーカーを用いたインドネシア在来ヤギの遺伝構造解析及び遺伝子流入の推定
- Apr. 2021 日本畜産学会, The 2021 Award for excellence in reviewing Animal Science JournalOfficial journal
- Mar. 2021 日本畜産学会, 優秀発表賞, 父母系および高密度 SNPマーカーを用いたフィリピン在来ヤギの遺伝構造と伝播の推定
- Nov. 2020 日本動物遺伝育種学会, 優秀発表賞, 集積流体回路を用いた兵庫県黒毛和種集団におけるロース脂肪割合候補多型の効果の検証
- Nov. 2020 日本動物遺伝育種学会, 優秀発表賞学会長特別賞, ネパール在来ヤギにおけるFGF5,EPAS1遺伝子多型と高地適応形質との関連調査
- Nov. 2019 日本動物遺伝育種学会, 優秀発表賞, 在来鶏および改良品種の遺伝的構造と遺伝的多様性に関する研究
- Nov. 2019 日本動物遺伝育種学会, 優秀発表賞, ミトゲノム配列による ハプログループT4の系統解析Japan society
- Mar. 2019 日本畜産学会, Animal Science Journal Reviewers Award, Animal Science Journalにおける審査Official journal
- Sep. 2018 日本動物遺伝育種学会, 日本動物遺伝育種学会優秀発表賞学会長特別賞, カザフスタン在来ヤギに対するmtDNAとY染色体の塩基配列解析～Eurasian Steppeは重要なヤギの伝播経路の一つである～Japan society
- Sep. 2018 日本動物遺伝育種学会, 日本動物遺伝育種学会優秀発表賞, 兵庫県黒毛和種における全ゲノムリシーケンスデータを用いた枝肉形質に対する候補変異の抽出Japan society
- Mar. 2018 日本畜産学会, 第124回日本畜産学会大会優秀発表賞, ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索Japan society
- Mar. 2018 日本畜産学会, Animal Science Journal Reviewers Award, Animal Science Journalにおける審査Official journal
- Sep. 2017 関西畜産学会, 第67回関西畜産学会優秀発表賞2, Indelマーカーを用いた家畜ウシ11集団に対する遺伝的構造および系統解析Japan society
- Sep. 2017 関西畜産学会, 第67回関西畜産学会優秀発表賞1, 北海道ホルスタインは希少mtDNAハプログループPを有するJapan society
- Mar. 2017 Japanese Society of Animal Science, Animal Science Journal Excellent Paper award, Genome Wide association study for fatty acid composition in Japanese Black cattleJapan society
- Nov. 2016 日本動物遺伝育種学会, 第17回日本動物遺伝育種学会大会・学会長特別賞, 黒毛和種繁殖雌牛集団におけるレプチン遺伝子内多型と繁殖形質との関連Japan society
- Mar. 2015 Japanese Society of Animal Science, ・Animal Science Journal Excellent paper award, Identification and utilization of genes associated with beef qualitiesJapan society
- Mar. 2015 Japanese Society of Animal Science, Animal Science Journal Excellent Paper award, Effect of DNA polymorphisms related to fatty acid composition in adipose tissueJapan society
- Nov. 2014 日本動物遺伝育種学会, 日本動物遺伝育種学会学会長特別賞, DigiTag2 assay を用いたアジア牛集団に対する遺伝的類縁関係と遺伝的構造の解析Japan society
- Oct. 2013 神戸大学農学部六篠会, 六篠教育貢献賞, 牛品種と牛肉品質を判別するウシゲノム診断技術の開発Others
- Mar. 2013 Japanese Society of Animal Science, Animal Science Journal Excellent paper award, Effect of SCD and SREBP genotypes on fatty acid composition in adipose tissue of Japanese Black cattle herdsJapan society
- Oct. 2012 神戸大学農学部六篠会, 六篠業績賞, アジアの在来家畜の起源・系統史研究と遺伝資源の先駆的汎利用Others
- Oct. 2012 神戸大学, 神戸大学学長表彰, ウシを中心とする家畜の遺伝育種学的研究Others
- Apr. 2012 文部科学省, The Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology, 牛品種と牛肉品質を判別するウシゲノム診断技術の開発
- Mar. 2012 日本畜産学会, Animal Science Journal Reviewers Award, 優秀論文
- Sep. 2011 関西畜産学会, 優秀発表賞, DigiTag2 アッセイを用いたウシ個体識別及び親子鑑定システムの確立
- Apr. 2011 日本農学会, 日本農学賞, アジアの在来家畜の起源・系統史研究と遺伝資源の先駆的汎利用
- Apr. 2011 読売新聞社, 読売農学賞, アジアの在来家畜の起源・系統史研究と遺伝資源の先駆的汎利用
- Mar. 2011 日本畜産学会, Animal Science Journal Reviewers Award, 優秀論文審査
- Jul. 2010 International Society of Animal Genetics, Excellent Poster Award, Polymorphisms of fat metabolism related genes and association between genotypes and carcass traits in Japanese Black cattle
- Mar. 2010 日本畜産学会, 日本畜産学会賞, 牛肉品質に関連する遺伝子の同定と利用
- Nov. 2006 日本動物遺伝育種学会, 学会長特別賞, ニワトリ筋ジストロフィー原因候補遺伝子の解析
- Nov. 2002 日本動物遺伝育種学会, 学会長特別賞, 黒毛和種ホルスタイン種との交雑種の品種鑑定に向けたAFLP法の適用
- Mar. 1995 日本畜産学会, 日本畜産学会奨励賞, DNAフィンガープリント法の家畜育種への応用
- Corresponding, Mar. 2025, Scientific Reports, 15, 9411, English, International magazine, Co-authored internationally[Refereed]Scientific journal
- Sep. 2024, Animal Science Journal, 95, e13999, English, International magazine, Co-authored internationally[Refereed]Scientific journal
- Springer Science and Business Media LLC, Apr. 2024, In Vitro Cellular & Developmental Biology - Animal, 60, 771 - 780, English, International magazine[Refereed]Scientific journal
- Abstract Background Beef marbling is considered a desirable trait in the meat industry. Therefore, understanding the genetic factors that cause marbling is important. Previously, we performed a genome-wide association study to examine genetic factors associated with beef marbling in Japanese Black cattle and identified a candidate region between 10–30 Mbp on chromosome 7. We verified the effect of the SNPs in this region on beef marbling using linkage disequilibrium block analysis. We narrowed down the candidate region to a range of 15.8–16.1 Mbp. In this study, we comprehensively detected all of the SNPs in this region and verified their effects on beef marbling. Results Genome resequencing using four animals exhibiting high beef marbling standard (BMS) and four with low BMS revealed a total of 1,846 polymorphisms within the candidate region. Based on the annotation, we selected 13 SNPs exhibiting a moderate impact, as no high-impact SNPs were detected. All of the SNPs represented missense polymorphisms and were located in the following seven genes: RDH8, ANGPTL6, DNMT1, MRPL4, ICAM1, ICAM3, and ICAM5. Finally, we determined the effects of these SNPs on the BMS of a Japanese Black cattle population (n = 529). Analysis of variance revealed that the five SNPs were located in genes encoding the intercellular adhesion molecules (ICAM1, ICAM3, and ICAM5), and showed a highly significant association compared with the remainder (p < 0.01). The lowest p-value was observed for ICAM3_c.739G > A (p = 1.18E-04). Previous studies have suggested that intercellular adhesion molecules (ICAM) may be an upstream factor that regulates adipocyte differentiation. Therefore, considering the polymorphism and putative gene function, we suggest that ICAM1 is potentially responsible for beef marbling. c.470C > G and/or c.994G > A on ICAM1 may be responsible for this quantitative trait locus. Conclusions Promising SNP candidates responsible for beef marbling were identified using extensive polymorphism verification in a previously reported QTL region. We aim to elucidate the mechanism of beef marbling in future studies by investigating how these polymorphisms alter protein structure and function.Springer Science and Business Media LLC, Feb. 2024, BMC Genomic Data, 25(1) (1), English[Refereed]Scientific journal
- In this study, the genetic parameters of major visceral diseases were estimated using the postmortem inspection records of 9057 fattening Japanese Black cattle in Shimane Prefecture, Japan, and the genetic correlation between visceral diseases and carcass traits was analyzed. There were six visceral diseases with a prevalence of 5% or higher, namely, pleurisy, pneumonia, bovine abdominal fat necrosis (BFN), rumenitis, hemorrhagic hepatitis, and perihepatitis. Variance components were estimated using the Gibbs sampling method, and the heritability of the visceral disease ranged from 0.07 to 0.49 for perihepatitis and BFN, respectively. Significant negative genetic correlations were identified between pleurisy and rib thickness (-0.32), BFN and carcass weight (-0.29), and BFN and rib eye area (-0.22). No significant genetic correlation was observed among the visceral diseases. The least squares analysis of variance suggested that some visceral diseases decrease the value of carcass traits. In particular, carcass weight and rib eye area in individuals with BFN were 11.7 kg and 1.87 cm2 lower than those of healthy cattle, respectively. Thus, it was inferred that genetic factors were involved in the visceral diseases of fattening Japanese Black cattle in Shimane Prefecture.Feb. 2024, Animal science journal = Nihon chikusan Gakkaiho, 95(1) (1), e13930, English, International magazine[Refereed]Scientific journal
- Since Malagasy human culture became established in a multi-layered way by genetic admixture of Austronesian (Indonesia), Bantu (East Africa) and West Asian populations, the Malagasy native livestock should also have originated from these regions. While recent genetic studies revealed that Malagasy native dogs and goats were propagated from Africa, the origin of Malagasy native chickens is still controversial. Here, we conducted a phylogeographic analysis of the native chickens, focusing on the historical relationships among the Indian Ocean rim countries and based on mitochondrial D-loop sequences. Although previous work suggested that the rare Haplogroup D occurs with high frequencies in Island Southeast Asia-Pacific, East Africa and Madagascar, the major mitochondrial lineage in Malagasy populations is actually not Haplogroup D but the Sub-haplogroup C2, which is also observed in East Africa, North Africa, India and West Asia. We demonstrate that the Malagasy native chickens were propagated directly from West Asia (including India and North Africa), and not via East Africa. Furthermore, they display clear genetic differentiation within Madagascar, separated into the Highland and Lowland regions as seen in the human genomic landscape on this island. Our findings provide new insights for better understanding the intercommunion of material/non-material cultures within and around Madagascar.Jan. 2024, Scientific reports, 14(1) (1), 569 - 569, English, International magazine, Co-authored internationally[Refereed]Scientific journal
- Jun. 2023, The 4th International Conference of Animal Science and Technology, 2628, 020003, English, International magazineOrigins and propagation routes of South/Southeast Asian goats analyzed by DNA information[Refereed][Invited]International conference proceedings
- Last, Elsevier BV, Apr. 2023, Small Ruminant Research, 221, 106937 - 106937, English, Co-authored internationally[Refereed]Scientific journal
- There are four unique cattle breeds in Japan: Japanese Black, Japanese Brown, Japanese Polled, and Japanese Shorthorn. The objective of this study was to comprehensively assess the genetic diversity, structure, relationship, and the degree of influence from foreign breeds (Angus, Simmental, Hanwoo, Shorthorn, Ayrshire, Brown Swiss, and Devon) in the Japanese cattle breeds using Illumina 50 K SNP array. In principal component analysis, each Japanese breed was separately clustered except for Japanese Shorthorn and Shorthorn. Japanese cattle breeds also showed different genetic components from each other at K ≥ 5 in population structure analysis. Japanese Shorthorn, on the other hand, had a very similar structure to Shorthorn at K ≤ 9, and Japanese Polled had a partially similar component with Angus at K = 3-7. Such close relationships were also observed in the phylogenetic tree. These findings imply that Japanese cattle breeds share genetic components with European cattle breeds to some extent while they have been almost differentiated. In population structure analysis, Japanese Black cattle shared little genetic component (3.5%) with European breeds. This is the first study to determine the extent to which European breeds impact Japanese breeds.Oct. 2022, Animal science journal = Nihon chikusan Gakkaiho, 93(1) (1), e13770, English, International magazine[Refereed]Scientific journal
- Wiley, Aug. 2022, Molecular Ecology, 31(16) (16), 4364 - 4380, English, International magazine, Co-authored internationally[Refereed]Scientific journal
- The elongation factor 1 alpha 1 (EEF1A1), an isoform of EEF1A, is one of the most abundant cytoplasmic proteins and an important component of the translational machinery. We investigated the relative expression, alternative polyadenylation (APA), and changes in poly(A) tail length of EEF1A1 mRNA in the endometrial caruncle (CAR) and intercaruncle (ICAR) at early and mid-gestation in Japanese Black cattle. The relative EEF1A1 mRNA expression levels in the CAR were the highest on Gestation day 20 and were significantly decreased at mid-gestation. The expression levels in the ICAR were significantly higher than those in the CAR, and the gestation stage had no significant impact. Four different EEF1A1 transcripts with distinct 3' untranslated regions (UTRs) (proximal and distal types) and poly(A) tails (medium and short types) of different lengths were identified. The EEF1A1 mRNAs with distal 3' UTR and medium-length poly(A) tails were specific from the CAR of uterus horn at early gestation. RNA-sequencing data analyses revealed that the HSF1, MZF1, E47, SRF, GATA2, GATA3, GATA6, HNF-3 beta (FOXA2), CPSF1, and Ataxin-2 genes might affect the EEF1A1 gene expression or poly(A) length.Jul. 2022, Animal science journal = Nihon chikusan Gakkaiho, 93(1) (1), e13746, English, International magazine[Refereed]Scientific journal
- In our previous study, we used genome resequencing to detect all candidate polymorphisms within a quantitative trait loci (QTL) region for beef marbling reported previously at 10–30 Mbp on bovine chromosome 7, and we selected 6044 polymorphisms as candidate quantitative trait nucleotides (QTNs). In the present study, we aimed to identify quantitative trait genes (QTGs) and QTNs in this QTL region by verifying the effect of SNPs on beef marbling in two Japanese Black cattle populations using a Dynamic Array integrated fluidic circuit. In total, 96 selected SNPs were genotyped in 441 and 529 animals in Hyogo and Miyazaki cattle populations, respectively. The most significant p-values were detected in a SNP in a splice region of ALDH7A1 (SNP93_ALDH7A1; p = 3.46 × 10−5) in Hyogo cattle and a missense polymorphism of intercellular adhesion molecule-1 (ICAM1) (SNP37_ICAM1; p = 3.33 × 10−4) in Miyazaki cattle. Interestingly, SNP93_ALDH7A1 was not significant (p = 0.459) in Miyazaki cattle, and SNP37_ICAM1 showed a weakly significant association (p = 0.043) in Hyogo cattle. Thus, each population would likely have different QTGs and QTNs for beef marbling in the QTL region. In the Hyogo population, it was not possible to determine the accurate range of the linkage disequilibrium (LD) block in LD block analysis because of a strong LD structure throughout the assessed region. In Miyazaki cattle, however, an LD block containing SNP37_ICAM1 had a range of 15.8–16.1 Mbp, suggesting that QTNs would be located within this region. The functions of 19 genes in the LD block were investigated. ICAM1 is known to play an important role in adipocyte differentiation; given this function and the effect of amino acid substitution, SNP37_ICAM1 was identified as a promising candidate QTN for beef marbling. Further research on the effect of SNP37_ICAM1 on adipocyte differentiation is expected to provide insights into the mechanism underlying beef marbling formation.Last, MDPI AG, Jul. 2022, Genes, 13(7) (7), 1190 - 1190, English, International magazine[Refereed]Scientific journal
- Bovine leukemia virus (BLV), the causative agent of enzootic bovine leukosis, is currently one of the most important pathogens affecting the cattle industry worldwide. Determining where and in which host it originated, and how it dispersed across continents will provide valuable insights into its historical emergence as the cattle pathogen. Various species in the Bos genus were domesticated in Asia, where they also diversified. As native cattle (taurine cattle, zebu cattle, yak, and water buffalo) are indigenous and adapted to local environments, we hypothesized that Asian native cattle could have harbored BLV and, therefore, that they were important for virus emergence, maintenance, and spread. In this study, phylogeographic and ancestral trait analyses—including sequences obtained from Asian native cattle—were used to reconstruct the evolutionary history of BLV. It was shown that, since its probable emergence in Asia, the virus spread to South America and Europe via international trade of live cattle. It was inferred that zebu cattle were the hosts for the early origin of BLV, while taurine cattle played the significant role in the transmission worldwide. In addition, the results of positive selection analysis indicate that yak had a substantially minor role in the transmission of this virus. In this study, endogenous deltaretrovirus sequences in bats, collected in Asian countries, were also analyzed on whether these sequences were present in the bat genome. Endogenous deltaretrovirus sequences were detected from bat species endemic to specific regions and geographically isolated for a long time. Endogenous deltaretrovirus sequences from these geographically isolated species represent ancient exogenous deltaretroviruses distributions. The phylogenetic analysis revealed that these newly obtained endogenous deltaretrovirus sequences were closely related to those of BLV from Asian native cattle, indicating that BLV-related ancient deltaretroviruses circulated in Asia long before the emergence of BLV. Together, our analyses provide evidence for origin and spatiotemporal dynamics of BLV.Frontiers Media SA, Jun. 2022, Frontiers in Microbiology, 13, English, International magazine, Co-authored internationally[Refereed]Scientific journal
- Jan. 2022, Frontiers in Genetics, 823364, English, International magazine, Co-authored internationallyOrigin and demographic history of Philippine pigs inferred from mitochondrial DNA[Refereed]Scientific journal
- Corresponding, Wiley, Sep. 2021, Animal Science Journal, 92(1) (1), English, International magazine, Co-authored internationally[Refereed]Scientific journal
- Aug. 2021, J. Anim. Genet., 49(2) (2), 67 - 75, EnglishRecent achievements of candidate polymorphism detection for fatty acied composition in Japanese Black cattle[Refereed]Scientific journal
- Five polymorphisms associated with the percentage of oleic acid (C18:1) in beef fat were previously reported on bovine chromosome 19 in different Japanese Black cattle populations. This study aimed to verify the effects of these five polymorphisms on C18:1 using the same Japanese Black cattle population and conduct linkage disequilibrium (LD) analysis in order to determine the locations of the quantitative trait loci (QTLs). We genotyped the five polymorphisms (SREBP1 c.1065 + 83 (84bp indel), STARD3 c.1187 C > T, GH c.379 C > G, FASN g.841 G > C, and FASN g.16024 A > G) in two populations, which were bred in Hyogo and Gifu Prefectures, Japan (n = 441 and 443, respectively) in order to analyze their effects on C18:1 using analysis of variance (ANOVA). In the Hyogo population, SREBP1 c.1065 + 83 and STARD3 c.1187 C > T were significantly associated with C18:1 (p < 0.001). Meanwhile, FASN g.841 G > C, FASN g.16024 A > G, and GH c.379 C > G were significantly associated with C18:1 (p < 0.01) in the Gifu population. LD analysis was subsequently conducted to detect the range of the QTLs, which ranged from 32.2 to 46.4 Mbp and from 47.8 to 52.1 Mbp in the Hyogo and Gifu populations, respectively. In conclusion, this study confirmed the existence of QTLs on BTA19 and divided the candidate region for each QTL based on LD coefficients. These results could contribute to efficient searches for responsible genes and polymorphisms for fatty acid composition.MDPI AG, Jun. 2021, Life, 11(7) (7), 597 - 597, English[Refereed]Scientific journal
- Objective: Conservation and genetic improvement of cattle breeds require information about genetic diversity and population structure of the cattle. In this study, we investigated the genetic diversity and population structure of the three cattle breeds in the Korean peninsula.Methods: Jeju Black, Hanwoo, Holstein cattle in Korea, together with six foreign breeds were examined. Genetic diversity within the cattle breeds was analyzed with minor allele frequency (MAF), observed and expected heterozygosity (HO and HE), inbreeding coefficient (FIS) and past effective population size. Molecular variance and population structure between the nine breeds were analyzed using a model-based clustering method. Genetic distances between breeds were evaluated with Nei’s genetic distance and Weir and Cockerham’s FST.Results: Our results revealed that Jeju Black cattle had lowest level of heterozygosity (HE = 0.21) among the studied taurine breeds, and an average MAF of 0.16. The level of inbreeding was –0.076 for Jeju Black, while –0.018 to –0.118 for the other breeds. Principle component analysis and neighbor-joining tree showed a clear separation of Jeju Black cattle from other local (Hanwoo and Japanese cattle) and taurine/indicine cattle breeds in evolutionary process, and a distinct pattern of admixture of Jeju Black cattle having no clustering with other studied populations. The FST value between Jeju Black cattle and Hanwoo was 0.106, which was lowest across the pair of breeds ranging from 0.161 to 0.274, indicating some degree of genetic closeness of Jeju Black cattle with Hanwoo. The past effective population size of Jeju Black cattle was very small, i.e. 38 in 13 generation ago, whereas 209 for Hanwoo.Conclusion: This study indicates genetic uniqueness of Jeju Black cattle. However, a small effective population size of Jeju Black cattle indicates the requirement for an implementation of a sustainable breeding policy to increase the population for genetic improvement and future conservation.Asian Australasian Association of Animal Production Societies, May 2021, Animal Bioscience, 34(5) (5), 789 - 800, English, International magazine, Co-authored internationally[Refereed]Scientific journal
- Springer Science and Business Media LLC, Nov. 2020, Scientific Reports, 10(1) (1), 20842, English, International magazine, Co-authored internationally
Abstract Surveys of mitochondrial DNA (mtDNA) variation have shown that worldwide domestic cattle are characterized by just a few major haplogroups. Two, T and I, are common and characterizeBos taurus andBos indicus , respectively, while the other three, P, Q and R, are rare and are found only in taurine breeds. Haplogroup P is typical of extinct European aurochs, while intriguingly modern P mtDNAs have only been found in northeast Asian cattle. These Asian P mtDNAs are extremely rare with the exception of the Japanese Shorthorn breed, where they reach a frequency of 45.9%. To shed light on the origin of this haplogroup in northeast Asian cattle, we completely sequenced 14 Japanese Shorthorn mitogenomes belonging to haplogroup P. Phylogenetic and Bayesian analyses revealed: (1) a post-glacial expansion of aurochs carrying haplogroup P from Europe to Asia; (2) that all Asian P mtDNAs belong to a single sub-haplogroup (P1a), so far never detected in either European or Asian aurochs remains, which was incorporated into domestic cattle of continental northeastern Asia possibly ~ 3700 years ago; and (3) that haplogroup P1a mtDNAs found in the Japanese Shorthorn breed probably reached Japan about 650 years ago from Mongolia/Russia, in agreement with historical evidence.[Refereed]Scientific journal - Abstract. Carcass traits have been efficiently improved by recent selection using DNA markers in beef cattle. Additionally, DNA markers might have an effect on other traits such as fertility traits; therefore attention should also be paid to such pleiotropic effects. However, the effects of the markers on both carcass and fertility traits have never been evaluated in the same population, since they are generally measured in different populations. The objective in the current study was to discuss effectiveness of DNA markers developed for carcass traits through investigation of their effects on carcass and fertility traits in a population. We genotyped six markers SCD V293A, FASN g.841G>C, PLAG1 g.49066C>G, NCAPG I442M, DGAT1 K232A, and EDG1 g.1471620G>T in a Japanese Black cattle population (n=515). To investigate their effects on carcass and fertility traits, we performed statistical analysis (ANOVA and the Tukey–Kramer honestly significant difference (HSD) test). In the results, three of six markers, SCD V293A, NCAPG I442M, and EGD1 g.1471620G>T, were significantly associated with both carcass and fertility traits. Remarkably, the same allele for each marker had positive effects on both traits, suggesting that we would be able to simultaneously improve them using these markers in this population. However, previous studies reported that the effects of DNA markers could differ among populations. Therefore, it is necessary to confirm the effect of the marker in each population before it is used for improvement.Copernicus GmbH, Jan. 2020, Archives Animal Breeding, 63(1) (1), 9 - 17, English, International magazine[Refereed]Scientific journal
- Wiley, Jan. 2020, Animal Science Journal, 91(1) (1), e13485, English, International magazine, Co-authored internationally[Refereed]Scientific journal
- Wiley, Jan. 2020, Animal Science Journal, 91(1) (1), e13335[Refereed]Scientific journal
- Meat quality in beef cattle is controlled by genetic factors. SPP1 (secreted phosphoprotein 1) gene, coding a multifunctional cytokine with diverse biological functions, is the candidate gene influencing carcass traits. In this study, we tried to discover DNA polymorphisms associated with beef quality in bovine SPP1 gene, so that two SNPs (single nucleotide polymorphisms) in the promoter region and one SNP in the CDS (coding sequence) region were identified. Although the formers were predicted to alter SPP1 expression, they did not show any effects on the traits. On the contrary, statistical analysis revealed that g.58675C > T, a non-synonymous mutation from threonine to methionine in the conservative region, had a significant effect on carcass weight. Carcass weight of the animals with C/T allele (473.9 ± 6.0 kg) was significantly heavier than that of the C/C homozygotes (459.2 ± 2.8 kg). Because SPP1 gene functions in skeletal muscle cells as a positive regulator, the non-synonymous mutation might influence muscle development and remodeling, resulting in increased carcass weight of the C/T animals. Our results indicate that the SNP can be applied as a DNA marker for the improvement of beef cattle.Dec. 2019, Heliyon, 5(12) (12), e03006, English, International magazine[Refereed]Scientific journal
- Sep. 2019, Warm Regional Society of Animal Science, 62(2) (2), 115 - 118, EnglishGenotyping of The FGD3 Gene in Japanese Black Cattle Bred in Oita Prefecture[Refereed]Scientific journal
- Aug. 2019, Journal of Animal Genetics, 47(2) (2), 37 - 41, EnglishEffect of STARD3 gene polymorphism on carcass traits and fatty acid composition in Japanese Black cattle[Refereed]Scientific journal
- Corresponding, Zoological Society of Japan, Aug. 2019, Zoological Science, 36(4) (4), 294 - 294, English, International magazine, Co-authored internationally[Refereed]Scientific journal
- Elsevier BV, Jun. 2019, Meta Gene, 20, 100558 - 100558, English[Refereed]Scientific journal
- Wiley, Apr. 2019, Animal Science Journal, 90(4) (4), 467 - 472, English[Refereed]Scientific journal
- (公社)日本畜産学会, Mar. 2019, 日本畜産学会大会講演要旨集, 125回, 177 - 177, Japanese複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討
- Corresponding, Wiley, Mar. 2019, Animal Science Journal, 90(3) (3), 317 - 322, English, International magazine, Co-authored internationally[Refereed]Scientific journal
- Corresponding, Wiley, Jan. 2019, Animal Science Journal, 90(1) (1), 29 - 34, English, International magazine, Co-authored internationally[Refereed]Scientific journal
- Local cattle breeds are popular in Myanmar because they make excellent draught animals, so it is of fundamental importance that their genetic diversities and population structures are elucidated for breeding and conservation purposes. In this study, we characterized the genetic diversities and population structures of four popular Myanmar local cattle breeds and five native cattle populations from neighboring countries (Bangladesh, Laos, Cambodia, and Vietnam) using the GGP Bovine 50K array. The mean observed and expected heterozygosity estimates using pruned datasets ranged from 0.317 and 0.322, respectively, in Cambodia to 0.448 and 0.421, respectively, in Vietnam South. The four Myanmar local breeds exhibited similar levels of genetic diversity. However, AMOVA revealed significant genetic differentiation among the nine populations (p < 0.00001), and Neighbor-Net analysis showed that Shan Ngwar Pu was distinct from the other Myanmar local breeds. Furthermore, PCA and population structure analyses revealed that the native cattle from neighboring countries genetically influenced the Myanmar local breeds to some extent and that the genetic origins could also be observed in the local breeds. These findings highlight the importance of Myanmar local breeds as genetic resources and provide useful information for the future development of breeding strategies and conservation management plans.Dec. 2018, Animal science journal = Nihon chikusan Gakkaiho, 89(12) (12), 1648 - 1655, English, International magazine, Co-authored internationally[Refereed]Scientific journal
- Corresponding, Aug. 2018, 動物遺伝育種研究, 46(2) (2), 49 - 55, Japanese日本ホルスタインにおいて観察された稀少なmtDNAハプログループP[Refereed]Scientific journal
- Wiley, Aug. 2018, Animal Science Journal, 89(8) (8), 1060 - 1066, English[Refereed]Scientific journal
- Aug. 2018, 日本畜産学会報, 89(3) (3), 313 - 321, JapaneseIndelマーカーを用いた家畜ウシ11集団に対する遺伝的構造および系統解析[Refereed]Scientific journal
- Aug. 2018, J. Anim. Genet., 46(2) (2), 57 - 67, English, International magazine, Co-authored internationallyGenetic diversity of Myanmar cattle breeds using complete mitochondrial D-loop sequence[Refereed]Scientific journal
- Wiley, May 2018, Animal Science Journal, 89(5) (5), 743 - 751, English[Refereed]Scientific journal
- (公社)日本畜産学会, Mar. 2018, 日本畜産学会大会講演要旨集, 124回, 111 - 111, Japaneseウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索
- Background Aurochs (Bos primigenius) were distributed throughout large parts of Eurasia and Northern Africa during the late Pleistocene and the early Holocene, and all modern cattle are derived from the aurochs. Although the mtDNA haplogroups of most modern cattle belong to haplogroups T and I, several additional haplogroups (P, Q, R, C and E) have been identified in modern cattle and aurochs. Haplogroup P was the most common haplogroup in European aurochs, but so far, it has been identified in only three of > 3,000 submitted haplotypes of modern Asian cattle. Methodology We sequenced the complete mtDNA D-loop region of 181 Japanese Shorthorn cattle and analyzed these together with representative bovine mtDNA sequences. The haplotype P of Japanese Shorthorn cattle was analyzed along with that of 36 previously published European aurochs and three modern Asian cattle sequences using the hypervariable 410 bp of the D-loop region. Conclusions We detected the mtDNA haplogroup P in Japanese Shorthorn cattle with an extremely high frequency (83/181). Phylogenetic networks revealed two main clusters, designated as Pa for haplogroup P in European aurochs and Pc in modern Asian cattle. We also report the genetic diversity of haplogroup P compared with the sequences of extinct aurochs. No shared haplotypes are observed between the European aurochs and the modern Asian cattle. This finding suggests the possibility of local and secondary introgression events of haplogroup P in northeast Asian cattle, and will contribute to a better understanding of its origin and genetic diversity.Public Library of Science, Jan. 2018, PLoS ONE, 13(1) (1), e0190937, English[Refereed]Scientific journal
- Wiley, Jan. 2018, Animal Science Journal, 89(1) (1), 257 - 258, English[Refereed]Scientific journal
- Genetics and Molecular Research, 2018, Genetics and Molecular Research, 17(3) (3), 18056, English[Refereed]Scientific journal
- Sep. 2017, Animal Veterinary Sciences, 5, 69 - 72, English[Refereed]Scientific journal
- Myanmar native horses are small horses used mainly for drafting carts or carriages in rural areas and packing loads in mountainy areas. In the present study, we investigated genotype distributions and allele frequencies of the LCORL/NCAPG, MSTN and DMRT3 genes, which are associated with body composition and locomotion traits of horses, in seven local populations of Myanmar native horses. The genotyping result of LCORL/NCAPG showed that allele frequencies of C allele associated with higher withers height ranged from 0.08 to 0.27, and 0.13 in average. For MSTN, allele frequencies of C allele associated with higher proportion of Type 2B muscular fiber ranged from 0.05 to 0.23, and 0.09 in average. For DMRT3, allele frequencies of A allele associated with ambling gait ranged from 0 to 0.04, and 0.01 in average. The presences of the minor alleles of these genes at low frequencies suggest a possibility that these horse populations have not been under strong selection pressure for particular locomotion traits and body composition. Our findings of the presence of these minor alleles in Southeast Asian native horses are also informative for considering the origins of these minor alleles associated with body composition and locomotion traits in horse populations.WILEY, Aug. 2017, ANIMAL SCIENCE JOURNAL, 88(8) (8), 1198 - 1203, English[Refereed]Scientific journal
- This study aims to estimate the mitochondrial genetic diversity and structure of Japanese Polled and Kuchinoshima feral cattle, which are maintained in small populations. We determined the mitochondrial DMA (mtDNA) displacement loop (D-loop) sequences for both cattle populations and analyzed these in conjunction with previously published data from Northeast Asian cattle populations. Our findings showed that Japanese native cattle have a predominant, Asian-specific mtDNA haplogroup T4 with high frequencies (0.43-0.81). This excluded Kuchinoshima cattle (32 animals), which had only one mtDNA haplotype belonging to the haplogroup T3. Japanese Polled showed relatively lower mtDNA diversity in the average sequence divergence (0.0020) than other Wagyu breeds (0.0036-0.0047). Japanese Polled have been maintained in a limited area of Yamaguchi, and the population size is now less than 200. Therefore, low mtDNA diversity in the Japanese Polled could be explained by the decreasing population size in the last three decades. We found low mtDNA diversity in both Japanese Polled and Kuchinoshima cattle. The genetic information obtained in this study will be useful for maintaining these populations and for understanding the origin of Japanese native cattle.WILEY, May 2017, ANIMAL SCIENCE JOURNAL, 88(5) (5), 739 - 744, English[Refereed]Scientific journal
- Previous studies have indicated that some leptin gene polymorphisms were associated with economically important traits in cattle breeds. However, polymorphisms in the leptin gene have not been reported thus far in Japanese Black cattle. Here, we aimed to identify the leptin gene polymorphisms which are associated with carcass traits and fatty acid composition in Japanese Black cattle. We sequenced the full-length coding sequence of leptin gene for eight Japanese Black cattle. Sequence comparison revealed eight single nucleotide polymorphisms (SNPs). Three of these were predicted to cause amino acid substitutions: Y7F, R25C and A80V. Then, we genotyped these SNPs in two populations (JB1 with 560 animals and JB2 with 450 animals) and investigated the effects on the traits. Y7F in JB1 and A80V in JB2 were excluded from statistical analysis because the minor allele frequencies were low (<0.1). Association analysis revealed that Y7F had a significant effect on the dressed carcass weight in JB2; R25C had a significant effect on C18:0 and C14:1 in JB1 and JB2, respectively; and A80V had a significant effect on C16:0, C16:1, C18:1, monounsaturated fatty acid and saturated fatty acid in JB1. The results suggested that these SNPs could be used as an effective marker for the improvement of Japanese Black cattle.WILEY, Mar. 2017, ANIMAL SCIENCE JOURNAL, 88(3) (3), 433 - 438, English[Refereed]Scientific journal
- Japanese Society of Animal Breeding and Genetics, 2017, The Journal of Animal Genetics, 45(1) (1), 3 - 8, Japanese[Refereed]Scientific journal
- 日本組織適合性学会, Oct. 2016, MHC: Major Histocompatibility Complex, 23(2Suppl.) (2Suppl.), 97 - 97, Japanese日本在来牛と黒毛和種のウシMHC領域のリシークエンスによる比較解析[Refereed]
- Japanese Society of Animal Science, Aug. 2016, 日本畜産学会報, 87(3) (3), 219 - 226, Japanese
Kuchinoshima cattle are unique feral cattle in Kuchinoshima Island in Kagoshima prefecture. We genotyped 54K SNPs for Kuchinoshima cattle, Japanese Black, Japanese Brown and Japanese Holstein using high density SNP chip to estimate genetic diversity and genetic structure in Kuchinoshima cattle. Average minor allele frequency in Kuchinoshima cattle was lower (0.089) than those of the other populations (0.181-0.251). Ratio of monomorphic SNPs for each chromosome in Kuchinoshima cattle ranged from 47.7% (BTA24) to 72.3% (BTA20). Subsequently, we investigated expected heterozygosity in each chromosome, and observed six monomorphic regions longer than 5Mb, especially, the longest region extended 12.80Mb. This study revealed the extremely low genetic diversity of Kuchinoshima cattle, due to founder effect derived from a few ancestral individuals and genetic drift caused by small population with below 100 animals lasting for a long time in Kuchinoshima Island.
[Refereed]Scientific journal - Consideration of the shortened fattening period seems to be worthwhile for the realization of profitable beef production. In this study, change of fatty acid composition of the lumbar longissimus during the final stage of fattening was investigated in Japanese Black cattle. Each of 110 fattening animals was sampled three times: the initial two samples were taken by biopsy (25.7 months and 27.5 months on average) and the final one was from carcasses (29.9 months on average). Preliminary analysis indicated that removing muscle tissues from the constant body position of the living animals should be essential for sampling. Average monounsaturated fatty acids (MUFA) at three sampling points were 58.1%, 58.5% and 60.5%, and the differences of the third sampling with the first and second samplings were significant. Both in steers and heifers, MUFA also increased as the fattening stage proceeded, and MUFA of the heifers at all the sampling points were significantly higher than those of the steers. The increasing rate of MUFA rose from 0.21 percentage points (pp)/month at period 1 (from the first sampling to the second sampling) to 0.84 pp/month at period 2 (from the second sampling to the slaughter).WILEY-BLACKWELL, Apr. 2016, ANIMAL SCIENCE JOURNAL, 87(4) (4), 578 - 583, English[Refereed]Scientific journal
- Marker-assisted selection (MAS) is expected to accelerate the genetic improvement of Japanese Black cattle. However, verification of the effects of the genes for MAS in different subpopulations is required prior to the application of MAS. In this study, we investigated the allelic frequencies and genotypic effects for carcass traits of six genes, which can be used in MAS, in eight local subpopulations. These genes are SCD, FASN and SREBP1, which are associated with the fatty acid composition of meat, and NCAPG, MC1R and F11, which are associated with carcass weight, coat color and blood coagulation abnormality, respectively. The frequencies of desirable alleles of SCD and FASN were relatively high and that of NCAPG was relatively low, and NCAPG was significantly associated with several carcass traits, including carcass weight. The proportions of genotypic variance explained by NCAPG to phenotypic variance were 4.83 for carcass weight. We thus confirmed that NCAPG is a useful marker for selection of carcass traits in these subpopulations. In addition, we found that the desirable alleles of six genes showed no negative effects on carcass traits. Therefore, selection using these genes to improve target traits should not have negative impacts on carcass traits.WILEY, Apr. 2016, ANIMAL SCIENCE JOURNAL, 87(4) (4), 469 - 476, English[Refereed]Scientific journal
- In this study, we identified a cluster of 14 avian -defensins (AvBD; approximately 66 kbp) in the Japanese quail, Coturnix japonica. Except for AvBD12 (CjAvBD12) and -13, the CjAvBDs coding sequences exhibited greater than 78.0% similarity to the respective orthologous chicken AvBD genes (GgAvBD). The putative amino acid sequence encoded by each CjAvBD contained six cysteine residues and the GXC (X1-2) motif considered essential for the -defensin family. Each CjAvBDs also formed a sub-group with the respective orthologous genes of various bird species in a phylogenetic tree analysis. Synteny between the CjAvBD cluster and GgAvBD cluster was confirmed. The CjAvBD cluster was mapped on the long-arm end of chromosome 3 by linkage analysis based on single nucleotide polymorphisms (SNPs) of CjAvBD1 and CjAvBD12 (approximately 46kbp), as well as GgAvBD cluster. We also confirmed that CjAvBD1, -4, -5, -9, and -10 are transcribed in 20 tissues, including immune and digestive tissues. However, our experimental data indicated that the CjAvBD cluster lacks the AvBD3 and -7 loci, whereas the CjAvBD101, -101, and -101 loci arose from gene duplication of the AvBD6 orthologous locus in the CjAvBD cluster after differentiation between Coturnix - Gallus.WILEY-BLACKWELL, Mar. 2016, ANIMAL SCIENCE JOURNAL, 87(3) (3), 311 - 320, English[Refereed]Scientific journal
- In this study, we genotyped 117 autosomal single nucleotide polymorphisms using a DigiTag2 assay to assess the genetic diversity, structure and relationships of 16 Eurasian cattle populations, including nine cattle breeds and seven native cattle. Phylogenetic and principal component analyses showed that Bos taurus and Bos indicus populations were clearly distinguished, whereas Japanese Shorthorn and Japanese Polled clustered with European populations. Furthermore, STRUCTURE analysis demonstrated the distinct separation between Bos taurus and Bos indicus (K=2), and between European and Asian populations (K=3). In addition, Japanese Holstein exhibited an admixture pattern with Asian and European cattle (K=3-5). Mongolian (K=13-16) and Japanese Black (K=14-16) populations exhibited admixture patterns with different ancestries. Bos indicus populations exhibited a uniform genetic structure at K=2-11, thereby suggesting that there are close genetic relationships among Bos indicus populations. However, the Bhutan and Bangladesh populations formed a cluster distinct from the other Bos indicus populations at K=12-16. In conclusion, our study could sufficiently explain the genetic construction of Asian cattle populations, including: (i) the close genetic relationships among Bos indicus populations; (ii) the genetic influences of European breeds on Japanese breeds; (iii) the genetic admixture in Japanese Holstein, Mongolian and Japanese Black cattle; and (iv) the genetic subpopulations in Southeast Asia.WILEY-BLACKWELL, Feb. 2016, ANIMAL SCIENCE JOURNAL, 87(2) (2), 190 - 196, English[Refereed]Scientific journal
- A missense mutation in the gene encoding WWP1 was identified as the most promising candidate responsible for chicken muscular dystrophy (MD) by genetic link-age analysis. WWP1 is a HECT-type E3 ubiquitin protein ligase composed of 922 amino acids, which contains 4 tandem WW domains that interact with the proline-rich peptide motifs of target proteins. The missense mutation changes arginine 441 that is located in the centre of the WW domains into glutamine (R441Q), which potentially affects the function of the WWP1 protein. Here, we show that WWP1 is detected as similar to 130-kDa protein that localizes to various structures, such as the plasma membrane (sarcolemma), sarcoplasmic reticulum, mitochondria and nucleus, in normal chicken skeletal muscle. However, in MD chickens, the mutant WWP1 protein was markedly degraded and was absent in the sarcolemma. These changes were also observed in the muscles of chickens in early pre-pathological states. Moreover, in vitro expression analysis showed significant degradation of mutant, but not wild-type WWP1, specifically in myogenic cells. Altogether, our data revealed that the R441Q missense mutation in the WWP1 protein causes degradation and loss of the sarcolemmal localization of WWP1, which may play a role in the pathogenesis of chicken MD.OXFORD UNIV PRESS, Feb. 2016, JOURNAL OF BIOCHEMISTRY, 159(2) (2), 171 - 179, English[Refereed]Scientific journal
- Japanese Society of Animal Breeding and Genetics, 2016, The Journal of Animal Genetics, 44(1) (1), 1 - 1, Japanese
- Japanese Society of Animal Science, 2016, Nihon Chikusan Gakkaiho, 87(4) (4), 333 - 338, Japanese[Refereed]Scientific journal
- The objective of the current study is to evaluate the association between fatty acid composition and fatty acid synthase gene polymorphisms as responsible mutations. For this purpose, we selected seven previously reported single nucleotide polymorphisms (SNPs) in FASN gene, including one within promoter region (g.841G>C) and six non-synonymous SNPs (g.8805C>T, g.13126C>T, g.15532A>C, g.16024A>G, g.16039C>T, g.17924A>G), and genotyped them in Japanese Black cattle. Genotyping results revealed that g.8805 C>T and g.17924 A>G were monomorphic loci. Genome-wide association analysis including the other five SNPs revealed that only g.841G>C showed significant associations with the percentages of C14:0, C14:1, C16:1 and C18:1 at 5% genome-wide significance level. In order to further evaluate the effect, we genotyped g.841G>C using additional three populations, including two Japanese Black populations and a Holstein cattle population. g.16024A>G was also genotyped and included in the analysis because it has been reported to be associated with fatty acid composition in Japanese Black cattle. In the result of analysis of variance, g.841G>C showed stronger effects on fatty acid percentage than those of g.16024A>G in all populations. These results suggested that g.841G>C would be a responsible mutation for fatty acid composition and contribute to production of high-grade beef as a selection marker in beef cattle.WILEY-BLACKWELL, Aug. 2015, ANIMAL SCIENCE JOURNAL, 86(8) (8), 737 - 746, English[Refereed]Scientific journal
- This study was a first analysis of paternal genetic diversity for extensive Asian domestic goats using SRY gene sequences. Sequencing comparison of the SRY 3-untranslated region among 210 Asian goats revealed four haplotypes (Y1A, Y1B, Y2A and Y2B) derived from four variable sites including a novel substitution detected in this study. In Asian goats, the predominant haplotype was Y1A (62%) and second most common was Y2B (30%). Interestingly, the Y2B was a unique East Asian Y chromosomal variant, which differentiates eastern and western Eurasian goats. The SRY geographic distribution in Myanmar and Cambodia indicated predominant the haplotype Y1A in plains areas and a high frequency of Y2B in mountain areas. The results suggest recent genetic infiltration of modern breeds into South-East Asian goats and an ancestral SRY Y2B haplotype in Asian native goats.WILEY-BLACKWELL, Jun. 2015, ANIMAL GENETICS, 46(3) (3), 337 - 339, English[Refereed]Scientific journal
- Allele frequency for c.335 A > C polymorphisms in porcine ghrelin/obestatin prepropeptide gene and association analysis with performance traits in various pig breedsThe allelic frequency of c.335A>C polymorphisms in the porcine ghrelin/obestatin prepropeptide (GHRL) gene was surveyed among six pig breeds and two subspecies of wild boars. The c.335C was the most frequent allele in Berkshire, Landrace, Large White, Yorkshire, and Clawn miniature pigs and Ryukyu wild boars (Sus scrofa riukiuanus), whereas c.335A was the most frequent allele in Duroc and Meishan pigs and Japanese wild boars (S. s. leucomystax). The association of c.335A>C with performance traits was analyzed in Duroc, Landrace, Large White, and (Landrace x Large White) x Duroc (LWD) cross-bred pigs. No associations between c.335A>C genotype and average daily weight gain, backfat thickness, or intramuscular fat were detected. However, an association was observed between loin eye muscle area (EMA) and c.335A>C genotype in Duroc gilts. The AA genotype group had larger EMA than the AC genotype group in Duroc gilts; however, this association was not significant in Duroc boars and barrows or the other pig populations investigated. These results demonstrate that GHRL c.335A>C is not a major quantitative trait loci candidate on pig chromosome 13 affecting fat deposition.CZECH ACADEMY AGRICULTURAL SCIENCES, 2015, CZECH JOURNAL OF ANIMAL SCIENCE, 60(9) (9), 411 - 416, English[Refereed]Scientific journal
- Oct. 2014, 日本マーケティング学会大会紀要集, Japanese豪州産WAGYUの海外市場流通状況把握と、DNAチップ技術によるオンサイト品種検査体制整備についての検討 （共著）[Refereed]Symposium
- 日本動物遺伝育種学会, Jul. 2014, 動物遺伝育種研究, 42(2) (2), 11 - 19, Japanese口之島牛集団における経済形質、遺伝性疾患および毛色に関連する遺伝子の対立遺伝子頻度とその分布[Refereed]Scientific journal
- Fatty acid composition of beef adipose tissue is one of its important traits because a high proportion of monounsaturated fatty acid is related to favorable beef flavor and tenderness. In this study, we searched polymorphisms in full length coding DNA sequence of urotensin 2 recepter and investigated the effects on fatty acid composition (C14:0, C14:1, C16:0, C16:1, C18:0, C18:1, C18:2, monounsaturated fatty acid, saturated fatty acid). Eight single nucleotide polymorphisms (SNP) were identified by sequence comparison among eight animals, including five Japanese Black and three Holstein cattle. One of these SNP (c.866C>T) was predicted to cause amino acid substitutions (P289L) and the other seven synonymous SNP, including c.267C>T, were presumed to be in linkage disequilibrium. Therefore we selected two SNP (c.267C>T and c.866C>T) for further analysis. We investigated associations between these genotypes and fatty acid composition in three Japanese Black populations (n=560, 245 and 287) and a Holstein population (n=202). Tukey-Kramer's honestly significant difference test revealed that CC genotype in c.267C>T indicated lower C14:0 and higher C18:1 than the other genotypes in Japanese Black cattle and CC genotype in c.866C>T showed lower C16:1 than CT genotype in Holstein cattle (P<0.05). These results suggested that these genotypes would contribute to production of high-grade meat as selection markers in beef cattle.WILEY-BLACKWELL, May 2014, ANIMAL SCIENCE JOURNAL, 85(5) (5), 499 - 505, English[Refereed]Scientific journal
- Genetic factors are known to influence meat quality in beef cattle. Expression of fatty acid desaturase 2 (FADS2) and fatty acid-binding protein 4 (FABP4) genes in the fat metabolism pathway has been reported to influence fat-related traits. In this study, we aimed to discover DNA polymorphisms in the promoter regions of the FADS2 and FABP4 genes in cattle populations to develop genetic markers. As a consequence, one single nucleotide polymorphism (SNP) in the FADS2 promoter (FADS2 g.-823G > A) and two linked SNPs in the FABP4 promoter (FABP4 g.-295A > G and FABP4 g.-287A > G) were identified. All of them were assumed to influence binding sites for transcription factors, suggesting these SNPs influence the genes' expression and beef quality. Therefore, we performed association analysis between the genotypes and traits. Our results revealed that the FADS2 g.-823G > A had significant effects on several traits including beef marbling score (BMS). BMS of the G/G animals in a Holstein population (2.36) was higher than that of the G/A animals (2.13). The same tendency was observed in Japanese Black populations. On the other hand, although the FABP4 SNPs also had significant effects on some traits, they did not show additive effects on any of the traits analyzed in this study. Our results suggest that the FADS2 g.-823G > A is a useful genetic marker for breeding in beef cattle. (C) 2014 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, May 2014, LIVESTOCK SCIENCE, 163, 34 - 40, English[Refereed]Scientific journal
- Applying DNA Chip Technology to Distribution of Agricultural ProductsToshiba invented an electrical current detection type of DNA (deoxyribonucleic acid) chip technology, and holds the basic patent. However, penetration has not been quick. The reason lies in the difficulties found in the business model for medical DNA testing of the human body. Difficulties concerning a social consensus on genetic testing, finding applications, and pharmaceutical approval are major cost factors. So the Ministry of Agriculture, Forestry and Fisheries, Toshiba, and several researchers plan to apply this technology to value-added branding for agricultural product exports to create more business opportunities. Another aim is to improve agricultural productivity by prevent highly infectious diseases. A questionnaire survey of agricultural producers was completed, and more than 70% of respondents supported application of this technology to agriculture. However, where should this technology be introduced in the very long supply chain of the agricultural export market? According to our findings, we should try to introduce this technology into wholesaler sectors of local governments in Japan, as well as quarantine authorities at export destination. This finding offers significant suggestion for thinking about where the technology should be inserted in the supply chain in order to promote market penetration.IEEE, 2014, 2014 PORTLAND INTERNATIONAL CONFERENCE ON MANAGEMENT OF ENGINEERING & TECHNOLOGY (PICMET), 3091 - 3094, English[Refereed]International conference proceedings
- Effects of genes on economically important traits of Japanese Black cattle in Hyogo populationIn this study, we investigated allelic frequencies and genetic effects of SCD, SREBP-1, FASN, EDG-1, F11, MC1R and CW-2 genes on beef carcass traits including fatty acid composition in the closed Hyogo population of Japanese Black cattle. The favorable allele frequencies were 0.960 in SCD, 0.322 in SREBP-1, 0.910 in FASN, 0.265 in EDG-1, 0.623 in F11, 0.464 in MC1R and 0.007 inNihon Chikusan Gakkai, Nov. 2013, Nihon Chikusan Gakkaiho, 84(2) (2), 157 - 162, Japanese[Refereed]Scientific journal
- Mitochondrial genetic diversity of goat in South Eastern AsiaIn this study, we determined 481bp hypervariable sequence of mitochondrial DNA D-loop region for Bangladeshi and Philippine native goats. The mitochondrial genetic diversity was estimated using obtained sequences, and then the origins of predominance mitochondrial lineage A and B in South East Asia were investigated. The analyses of 53 Bangladesh and 30 Philippine goats revealeNihon Chikusan Gakkai, Nov. 2013, Nihon Chikusan Gakkaiho, 84(2) (2), 149 - 155, Japanese[Refereed]Scientific journal
- Fatty acid composition is one of the important traits in beef. The aim of this study was to identify candidate genomic regions for fatty acid composition by genome-wide association study with 50K single nucleotide polymorphism (SNP) array in Japanese Black cattle. A total of 461 individuals and 40657 SNPs were used in this study. We applied genome-wide rapid association using mixed model and regression (GRAMMAR) and genomic control approaches to estimate the associations between genotypes and fatty acid composition. In addition, two SNPs in fatty acid synthase (FASN) (T1952A) and stearoyl-CoA desaturase (SCD) (V293A) genes were also genotyped. Association analysis revealed that 30 significant SNPs for several fatty acids (C14:0, C14:1, C16:1 and C18:1) were located in the BTA19FASN gene located within this region but the FASN mutation had no significant effect on any traits. We also detected one significant SNP for C18:1 on BTA23 and two SNPs for C16:0 on BTA25. The region around 17Mb on BTA26 harbored two significant SNPs for C14:1 and SNP in SCD in this region showed the strongest association with C14:1. This study demonstrated novel candidate regions in BTA19, 23 and 25 for fatty acid composition.WILEY-BLACKWELL, Oct. 2013, ANIMAL SCIENCE JOURNAL, 84(10) (10), 675 - 682, English[Refereed]Scientific journal
- Japanese Black cattle are at risk for genetic homogeneity due to intensive use of a few sires. Therefore, assessment of the actual genetic diversity of this breed is important for future breeding plans. In the present study, we investigated the genetic diversity within and among eight subpopulations of Japanese Black cattle using 52 microsatellite markers. The parameters for genetic diversity of Japanese Black cattle were comparable to those of other cattle breeds, suggesting that the relatively high genetic diversity of the breed. However, upon comparison among the eight subpopulations, the Hyogo subpopulation showed markedly low genetic diversity. The results of the pairwise F-ST values, phylogenetic network and structure analysis indicated that the Hyogo population has remarkably high level of genetic differentiation from other populations, while Yamagata, Niigata, Hiroshima and Kagawa populations have low levels of genetic differentiation. Furthermore, multidimensional scaling plots indicated that individuals in some subpopulations were separated from individuals in the other subpopulations. We conclude that while the overall genetic diversity of Japanese Black cattle is still maintained at a relatively high level, that of a particular subpopulation is significantly reduced, and therefore the effective population size of the breed needs to be controlled by correct mating strategies.WILEY-BLACKWELL, Aug. 2013, ANIMAL SCIENCE JOURNAL, 84(8) (8), 585 - 591, English[Refereed]Scientific journal
- In our previous study, we examined divergently selected regions between Japanese Black cattle and Holstein cattle based on a 50k single nucleotide polymorphism (SNP) panel and a Sliding Window Allele Difference method resulting in the identification of 11 genomic regions. The aim of the present study was to investigate the association between these genomic regions and economic traits, including seven carcass and five milk production traits. For this purpose, representative SNP markers were selected from the 11 genomic regions and used to estimate the effects on the traits in Japanese Black cattle (N=488) and Holstein cattle (N=194). Association analyses revealed that five SNPs showed a significant effect on the carcass traits in Japanese Black cattle and other five SNP showed a significant association with milk production traits in Holstein cattle (P< 0.05). These results indicated that divergently selected regions identified using a Sliding Window Allele Difference method contain the quantitative trait loci (QTL) for economic traits. Furthermore, most of the QTL identified in this study were consistent with the previously reported QTL. Further investigations of these regions may lead to the identification of the genes and polymorphisms responsible for the economic traits. © 2013 Elsevier B.V.Elsevier, Aug. 2013, Livestock Science, 155(2-3) (2-3), 180 - 185, English[Refereed]Scientific journal
- In the current study, a total of 65 single nucleotide polymorphisms (SNPs) within the intron region were developed in goat (Capra hircus) by utilizing genomic information of cattle and sheep due to poor available genomic information on goat. Using these markers, we carried out genetic diversity and structure analyses for 10 Asian goat populations. The phylogenetic tree and principal components analysis showed good correspondence between clustered populations and their geographic locations. The STRUCTURE software analysis illustrated six divergent genetic structures among 10 populations. Myanmar and Cambodia populations showed high admixture patterns with different ancestry, suggesting genetic introgression into native goat populations. We also investigated the correlation between genetic diversity and geographic distance from a domestication center. This result showed a decreasing trend of genetic diversity according to the distance (P = 0.014). This result supported common consensus that western Asia is one of the centers of origin for modern Asian domestic goat.WILEY-BLACKWELL, Aug. 2013, ANIMAL SCIENCE JOURNAL, 84(8) (8), 579 - 584, English[Refereed]Scientific journal
- Comparative analysis on gene expression profiles in longissimus dorsi muscle of Japanese Black cattleRecently, fatty acid composition has been recognized as an important economic trait of Japanese Black cattle. The aim of this study was to seek for candidate genes which might be useful for beef breeding to increase the mono-unsaturated fatty acid (MUFA) level. For this purpose, we compared gene expression profile of Japanese Black cattle which showed high percentage of MUFA in日本動物遺伝育種学会, Jun. 2013, The Journal of Animal Genetics, 41(1) (1), 7 - 14, Japanese[Refereed]Scientific journal
- Genetic analyses have contributed to improvements of economically important traits derived from adipose tissue such as fatty acid composition in beef. Elongation of very long chain fatty acids (ELOVL) genes encode for the enzymes that play an important role in elongation of long-chain fatty acids. In this study, we aimed to discover genetic polymorphisms of ELOVL gene family in cattle populations to develop genetic markers. As a result, five synonymous mutations were detected in the coding regions of the ELOVL1, ELOVL2, ELOVL3 and ELOVL5 genes. In addition, six mutations were identified in promoter region of the ELOVL5. Two of five mutations in the promoter region of ELOVL5 were expected to alter the ELOVL5 expression and influence the economic traits, because of the high synteny of the region which was essential for activation of Elovl5 in mouse. Therefore, we performed association analysis between the genotypes and traits and our result revealed that T allele of g.-110T > C in ELOVL5 gene promoter indicated significantly thinner subcutaneous fat thickness (TT, 2.39 cm; CT, 2.35 cm) than that of C allele (CC, 2.68 cm) in a Japanese Black population. Our results suggest that the g.-110T > C is a useful genetic marker for the breeding in beef cattle.SPRINGER, Apr. 2013, MOLECULAR BIOLOGY REPORTS, 40(4) (4), 3231 - 3237, English[Refereed]Scientific journal
- The domestic goat is one of the most important livestock species, but its origins and genetic diversity still remain uncertain. Multiple highly divergent maternal lineages of goat have been reported in previous studies. Although one of the mitochondrial DNA lineages, lineage B, was detected only in eastern and southern Asia, the geographic distribution of these lineages was previously unclear. Here, we examine the genetic diversity and phylogeographic structure of Asian goats by mitochondrial DNA sequences and morphological characteristics. The analyses of a total of 1661 Asian goats from 12 countries revealed a high frequency of lineage B in Southeast Asia. The frequency of this lineage tended to be higher in mountain areas than in plain areas in Southeast Asian countries, and there was a significant correlation between its frequency and morphological traits. The results suggest an original predominance of lineage B in Southeast Asia and the recent infiltration of lineage A into Southeast Asian goats.WILEY-BLACKWELL, Feb. 2013, ANIMAL GENETICS, 44(1) (1), 79 - 85, English[Refereed]Scientific journal
- Genome-wide association mapping for complex traits in cattle populations is a powerful, but expensive, selection tool. The DNA pooling technique can potentially reduce the cost of genome-wide association studies. However, in DNA pooling design, the additional variance generated by pooling-specific errors must be taken into account. Therefore, this study aimed to investigate factors such as: (i) the accuracy of allele frequency estimation; (ii) the magnitude of errors in pooling construction and in the array; and (iii) the effect of the number of replicate arrays on P-values estimated by a genome-wide association study. Results showed that the Illumina correction method is the most effective method to correct the allele frequency estimation; pooling errors, especially array variance, should be taken into account in DNA pooling design; and the risk of a type I error can be reduced by using at least two replicate arrays. These results indicate the practical capability and cost-effectiveness of pool-based genome-wide association studies using the BovineSNP50 array in a cattle population.WILEY-BLACKWELL, Nov. 2012, ANIMAL SCIENCE JOURNAL, 83(11) (11), 719 - 726, English[Refereed]Scientific journal
- Fatty acid composition is an important economic trait for both dairy and beef cattle and controlled by genetic factors. Candidate genes controlling fatty acid composition may be found in fat synthesis and metabolism pathways. Acetyl-CoA carboxylase is the flux-determining enzyme in the regulation of fatty acid synthesis in animal tissues. One of two isozymes of this enzyme, acetyl-CoA carboxylase-alpha (ACACA), catalyses the first committed step of fatty acid synthesis in mammalian cytosol, leading to the biosynthesis of long-chain fatty acids. In the current study, the sequence comparison of the coding sequence (CDS) and two promoter regions (PIA and PIII) in bovine ACACA gene was performed between Japanese Black and Holstein cattle to detect nucleotide polymorphisms influencing fatty acid composition in milk and beef. Five single nucleotide polymorphisms (SNPs) were identified in the CDS region, 28 SNPs in the PIA region and three SNPs in the PIII region. Association study revealed that CCT/CCT type of PIII_#1, #2/PIA_#26 indicated a higher percentage of C14:0 in the milk of the Holstein cattle than CCT/GTC type (p = 0.050) and that a difference of the percentage of C16:0 was observed between CCT/CCT and GTC/GTC type (p = 0.023). CDS_#2 T/T type indicated a higher percentage of C18:0 than T/C type (p = 0.008). In addition, the Japanese Black cattle with CC/GT type of PIII_#1, #2 showed a higher percentage of C18:2 in the meat than those with GT/GT type (p = 0.025). Since PIII is the promoter specific to mammary gland during lactation, the altered expression of the ACACA gene owing to the SNPs in the PIII region may influence the fatty acid composition in the milk.SPRINGER, Sep. 2012, MOLECULAR BIOLOGY REPORTS, 39(9) (9), 8637 - 8644, English[Refereed]Scientific journal
- FASN is a multifunctional enzyme that catalyzes de novo biosynthesis of long-chain saturated fatty acid, and alteration of this enzyme may affect economic traits including fatty acid composition. Sequencing the coding sequence (CDS) of the FASN gene has so far been done only partially for dairy cattle. In this study, the CDS of the FASN gene of eight Holstein cattle was sequenced to detect nucleotide polymorphisms. We could identify 13 single nucleotide polymorphisms (SNPs) of which five were non-synonymous mutations (H1390Y, I1856L, T1950A, W1955R and T2264A). The association between these SNPs and economic traits was analyzed using 198 Holstein dams. Genotypes of H1390Y corresponded to those of the I1856L in the group, suggesting these SNPs were linked. Furthermore, the T1950A was suggestively linked with the W1955R. The association study revealed that T1950A/W1955R had an effect on milk fat content and the C14 Index. This will help to understand the complex association between the FASN gene and economic traits. (C) 2011 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, Apr. 2012, LIVESTOCK SCIENCE, 144(3) (3), 281 - 284, English[Refereed]Scientific journal
- Differences between average allelic frequencies of genes that relate to traits suggest that it would be evidence of artificial selections. Sliding window approach is a useful method to identify genomic regions that have been differently selected between two breeds. The objective of this study was to identify the divergently selected regions between Japanese Black (JB) and Japanese Holstein (JH) cattle based on genotypic information obtained through a high-density single nucleotide polymorphism (SNP) panel. After genotyping of 54 001 SNP markers on 100 animals (50 JB and 50 JH), 40 635 SNPs were suitable for the analysis. For each of these SNPs, the absolute difference between allelic frequencies of JB and JH was calculated. In the current study, 10 consecutive SNPs were defined as components of a window. For each window, the average difference in allelic frequency was calculated. This was termed sliding window average difference (SWAD). Among 40 055 windows, we focused on 39 windows with the largest SWAD. This was equivalent to 0.1% of all windows and the SWAD was more than 0.435. Some of these windows overlapped and were distributed in 11 regions. These regions were in good agreement with reported quantitative trait locus, therefore would be selection signatures and good candidates that harbor the causative mutations.WILEY-BLACKWELL, 2012, ANIMAL SCIENCE JOURNAL, 83(1) (1), 7 - 13, English[Refereed]Scientific journal
- 日本畜産学会, Nov. 2011, 日本畜産学会報, 82(2) (2), 125 - 130, Japanese黒毛和種におけるDGAT1遺伝子多型の枝肉形質に対する効果[Refereed]Scientific journal
- The quality of fat is an important factor in defining the quality of meat. Fat quality is determined by the composition of fatty acids. Among lipid metabolism-related genes, including fatty acid synthesis genes, several genetic variations have been reported in the bovine fatty acid synthase (FASN), stearoyl-CoA desaturase (SCD), sterol regulatory element-binding protein 1 (SREBP1), and GH genes. In the present study, we evaluated the single and epistatic effects of 5 genetic variations (4 SNP and 1 insertion/deletion) in 4 genes (FASN, SCD, SREBP1, and GH) on the fatty acid composition of the longissimus thoracis muscle and carcass and meat quality traits in 480 commercial Japanese Black cattle. Significant single effects of FASN, SCD, and GH(L127V) polymorphisms on the fatty acid composition of the longissimus thoracis muscle were detected. The A293V polymorphism of SCD had the largest effect on myristic acid (C14:0, P < 0.001), myristoleic acid (C14:1, P < 0.001), stearic acid (C18:0, P < 0.001), oleic acid (C18:1, P < 0.001), and MUFA (P < 0.001). Polymorphisms in the FASN, SCD, and SREBP1 genes showed no effect on any meat yield trait. There were no significant epistatic effects on fatty acid composition among pairs of the 3 genes (FASN, SCD, and SREBP1) involved in fatty acid synthesis. No epistatic interactions (P > 0.1) were detected between FASN and SCD for any carcass trait. When the genotypes of 3 markers (FASN, SCD, and GH(L127V)) were substituted from the lesser effect allele to the greater effect allele, the proportion of C18:1 increased by 4.46%. More than 20% of the genetic variance in the C18: 1 level could be accounted for by these 3 genetic markers. The present results revealed that polymorphisms in 2 fatty acid synthesis genes (FASN and SCD) independently influenced fatty acid composition in the longissimus thoracis muscle. These results suggest that SNP in the FASN and SCD genes are useful markers for the improvement of fatty acid composition in commercial Japanese Black cattle.AMER SOC ANIMAL SCIENCE, Jan. 2011, JOURNAL OF ANIMAL SCIENCE, 89(1) (1), 12 - 22, English[Refereed]Scientific journal
- The electrophoretic variation in bovine hemoglobin-beta (HBB) is one of the most investigated genetic markers. The presence of a unique HBB variant, HBBX, in Southeast Asian cattle has been reputed as a sign of gene-flow from wild bovine species. In this study, we analyzed the DNA sequences of HBB genes in domestic and wild bovine species to verify this belief. Isoelectric focusing of HBB chain revealed that the HBBX in domestic cattle had dimorphism and was separated into HBBX1 and HBBX2. The HBBX1 had the same DNA sequence of the common HBB variant in gayal (Bos gaurus frontalis), while some of the HBBX2 were identical with that of Cambodian banteng (Bos javanicus birmanicus). As a result, we confirmed that the bovine HBB variants can be a good indicator of introgression between wild and domestic cattle. The HBBX1 was always predominant to HBBX2 in the continental populations, suggesting that the gaur had contributed to the gene pool of domestic cattle in this region much more than the banteng. On the other hand, the mitochondrial DNA analysis could not detect gene-flow from wild species. Autosomal markers that can trace the phylogeny between alleles are suitable for the assessment of bovine interspecific introgression.WILEY-BLACKWELL PUBLISHING, INC, 2011, ANIMAL SCIENCE JOURNAL, 82(1) (1), 36 - 45, English[Refereed]Scientific journal
- In the meat industry, correct labeling of beef origins or breed is required to assure quality and safety. This paper describes the development of discrimination markers between Japanese domestic and imported beef from the United States (US) and Australia (AUS) based on a bovine 50K single nucleotide polymorphism (SNP) array using a total of 110 samples: Japanese Black (n = 50), Japanese Holstein (n = 50) and US cattle (n = 10). Genotyping information revealed 1081 SNPs as candidate markers that were polymorphic only in US cattle. The genotyping results by PCR - restriction length polymorphism in Japanese Black (n = 300) and Holstein cattle (n = 146) revealed that 11 SNPs had alleles specific to US cattle. Their allelic frequencies in US cattle (n = 108) ranged from 0.097 to 0.250 with an average of 0.178 and the combined identification probability of US cattle was 0.987. In addition, we also verified the applicability of these US-specific markers to AUS cattle. Their allelic frequencies in AUS cattle (n = 280) ranged from 0.063 to 0.224 with an average of 0.137 and the combined identification probability of AUS cattle was 0.963. In conclusion, a set of these markers could be useful for discriminating between Japanese domestic and imported beef and would contribute to identify origins and prevent falsified labeling of beef.WILEY-BLACKWELL, 2011, ANIMAL SCIENCE JOURNAL, 82(1) (1), 67 - 72, English[Refereed]Scientific journal
- Fatty acid composition of adipose tissue has been recognized as an important carcass trait because of its relationship with eating quality such as favorable beef flavor and tenderness. Therefore, we investigated the effects of genetic polymorphisms of liver X receptor, alpha (LXR), stearoyl-CoA desaturase (SCD), Fatty acid synthase (FASN), and Fatty acid binding protein 4 (FABP4) on fatty acid composition in intramuscular fat tissue of Holstein steers. The major allele frequencies were 0.705 in SCD, 0.518 in FABP4, 0.888 in FASN, and 0.984 in LXR. Genotyping of SCD showed significant effect on C14:0, C14:1, C18:0 and saturated fatty acid (P < 0.05). In addition, the result suggested that SCD genotype possibly had effect on composition of C18:1 and monounsaturated fatty acid. Genotype of FABP4 had significant effect on composition of C16:0. Effect of LXR genotypes could not be analyze because of extremely biased genotype frequencies. Our results suggest that genotypes of SCD and FABP4 may in part affect meat quality in Holstein.WILEY-BLACKWELL, 2011, ANIMAL SCIENCE JOURNAL, 82(3) (3), 406 - 411, English[Refereed]Scientific journal
- Allele frequencies of 10 representative polymorphisms for beef and milk traits were investigated for a total of 240 animals from Bos taurus and Bos indicus breeds, including two Japanese groups (Japanese Black and Japanese Brown), two East Asian groups (Korean and Mongolian), three European groups (Holstein, Angus and Hereford) and a Bos indicus group in South Asia (Myanmar, Laos and Cambodia). The Japanese Black revealed unique genetic construction in GH, FASN and SREBP-1 and the other Asian populations show intermediate frequencies between European and Japanese populations. The Bos indicus group showed low favorable allele frequencies in most of the genes. The study showed the variability and distribution of 10 genes affecting economic traits among world representative cattle breeds. The genetic information would contribute to elucidating the genetic background for worldwide cattle breeds and the possibility of improvement using the markers.WILEY-BLACKWELL, 2011, ANIMAL SCIENCE JOURNAL, 82(6) (6), 717 - 721, English[Refereed]Scientific journal
- Suppression of WWP1 Gene Via RNAi Induced the Reduction of Proliferation Rate of C2C12 MyoblastsThe WW domain containing E3 ubiquitin protein ligase 1 (WWP1), which plays an important role in ubiquitin-proteasome pathway to degrade unneeded or damaged proteins, was recently identified as the responsible for chicken muscular dystrophy. Despite intensive studies on oncogenic characters, the role of WWP1 to muscular diseases has not yet been fully understood. Previously, we transfected either of wild and mutated types of WWP1 gene into C2C12 mouse myoblasts to monitor the expression pattern of muscle-differentiation markers, so that excessive WWP1 expression enhanced the expression of the myosin heavy chain (MyHC) Ia gene but lowered the expression of the MyHC IIb gene, while mutated WWP1 gene transfected in to myoblasts was distinct from these cases in that the MyHC gene or genes expression inhibited the normal myoblast differentiation. However, the mechanism for the mutation to inhibit muscle differentiation remains elucidated. The current study attempted to suppress the WWP1 expression by RNAi technique and to observe its effect on C2C12 cells. The effect of WWP1 suppression was clearly different from that of the R441Q missense mutation in the WWP1 gene. The WWP1 suppression reduced the proliferation rate of C2C12 myoblasts, while clear difference was not observed in the proliferation rate by the transfection of the mutated WWP1 gene into the cells. Our RT-PCR analysis indicated that the reduction of the WWP1 expression is the specific effect mediated by RNAi and that the reduction of proliferation rate observed in this study is largely attributed to the suppression of the WWP1 expression. These data indicated that the mutation responsible for chicken muscular dystrophy does not eliminate the enzymatic activity and provides some new function for the gene.JAPAN POULTRY SCIENCE ASSOC, Oct. 2010, JOURNAL OF POULTRY SCIENCE, 47(4) (4), 288 - 293, English[Refereed]Scientific journal
- Six polymorphic sites of the bovine prion protein gene (PRNP) were genotyped in 569 animals of Asian native cattle, Japanese breeds, purebred mythun (Bos frontalis), and mythun x cattle composite animals. At the 23-bp indel site, a deletion (23-) allele was a major allele in all populations except mythun. At the 12-bp indel site, an insertion (12+) allele was a major allele in all populations. The 14-bp indel site was polymorphic in all Asian native cattle. In the octapeptide repeat region, a six-repeat allele was a major allele in all populations, and 5/5 and 4/6 genotypes were detected in Japanese Black and Mongolian cattle and in mythun, respectively. Two nonsynonymous single nucleotide polymorphisms (SNPs) (K3T and S154N) were detected in Asian native cattle and mythun. Haplotype analysis using the genotypes of the six sites estimated 33 different haplotypes. The haplotype 23- 12- K 6 S 14+ was found in all populations.SPRINGER/PLENUM PUBLISHERS, Oct. 2010, BIOCHEMICAL GENETICS, 48(9-10) (9-10), 829 - 839, English[Refereed]Scientific journal
- Members of the caveolin family are the main component of caveolae, and caveolin-3 is a muscle-specific protein. Caveolin-3 deficiency induces a muscular dystrophic phenotype, while its overexpression is also harmful to muscle cells Increased caveolae were observed in chicken muscular dystrophy, however, the underlying mechanism causing the onset remains unclear Therefore, the current study analyzes the expression of caveolin-3 and other caveola-related proteins in dystrophic chickens. Western blotting and semi-quantitative RT-PCR analysis revealed that (1) caveolin-3 is highly expressed in the damaged muscle of dystrophic chickens (7 12-fold), (2) the amount of caveolin-3 protein is regulated in posttranslational modification, since no significant increase is observed at the mRNA level (1.09-fold), and (3) the expression pattern of other caveola-related proteins is similar to that of caveolin-3 These results suggest that the accumulation of caveolin-3 protein may be associated with the causative process of chicken muscular dystrophy. (C) 2010 Elsevier Inc. All rights reservedELSEVIER SCIENCE INC, Sep. 2010, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 157(1) (1), 68 - 72, English[Refereed]Scientific journal
- Japanese Society of Animal Genetics, Jun. 2010, Journal of Animal Genetics, 38(1) (1), 77 - 81, EnglishMitochondrial DNA variation and genetic construction of indigenous cattle population in Bhutan[Refereed]Scientific journal
- In Japan, Japanese Black and Holstein cattle are appreciated as popular sources of meat, and imported beef from Australia and the United States is also in demand in the meat industry. Since the BSE outbreak, the problem of false sales has arisen: imported beef has sometimes been mislabeled as domestic beef due to consumer concerns. A method is needed to correctly discriminate between Japanese and imported cattle for food safety. The objective of this study was to develop breed discrimination markers between Japanese and US cattle using a 50K SNP array. As a result, five US-specific markers (BISNP7, BISNP15, BISNP21, BISNP23, and BISNP26) were developed with allelic frequencies that ranged from 0.102 (BISNP15) to 0.250 (BISNP7) and averaged 0.184. The combined use of the five markers would permit discrimination between Japanese and US cattle with a probability of identification of 0.858. This result indicates the potential of the bovine 50K SNP array as a powerful tool for developing breed identification markers. These markers would contribute to the prevention of falsified beef displays in Japan. (C) 2010 Elsevier Ltd. All rights reserved.ELSEVIER SCI LTD, Jun. 2010, MEAT SCIENCE, 85(2) (2), 285 - 288, English[Refereed]Scientific journal
- The WW domain containing E3 ubiquitin protein ligase 1 (WWP1), an enzyme to degrade unneeded or damaged proteins, was recently identified as the responsible for chicken muscular dystrophy. Despite of intensive studies on oncogenic characters, the role of WWP1 to muscular diseases has not yet been fully understood. Since it is generally known that the switching of myosin heavy chain (MyHC) isoforms from neonatal isoform to adult one is inhibited in chicken muscular dystrophy, we transfected either of wild and mutated types of WWP1 gene into C2C12 cells to monitor the expression pattern of muscle-differentiation markers including MyHCs by real-time PCR. Excessive WWP1 expression enhanced the expression of the MyHC la gene but lowered the expression of the MyHC IIb gene. On the other hand, mutated WWP1 gene transfected into myoblasts was distinct from these cases in that the MyHC gene or genes expression inhibited the normal myoblast differentiation. The present data suggest that WWP1 promotes myoblast differentiation from embryonic into fast twitch phase while a mutation in WWP1 results to retain slow and fast twitch isoforms characteristic of dystrophic fast twitch muscles.JAPAN POULTRY SCIENCE ASSOC, Apr. 2010, JOURNAL OF POULTRY SCIENCE, 47(2) (2), 115 - 119, English[Refereed]Scientific journal
- International Society for Animal Genetics, Apr. 2010, Animal Genetics, 41(5) (5), 554 - 556, EnglishDiversity and phylogeny of mitochondrial DNA isolated from mithun Bos frontalis located in Bhutan[Refereed]Scientific journal
- Animal domestication was a major step forward in human prehistory, contributing to the emergence of more complex societies. At the time of the Neolithic transition, zebu cattle (Bos indicus) were probably the most abundant and important domestic livestock species in Southern Asia. Although archaeological evidence points toward the domestication of zebu cattle within the Indian subcontinent, the exact geographic origins and phylogenetic history of zebu cattle remains uncertain. Here, we report evidence from 844 zebu mitochondrial DNA (mtDNA) sequences surveyed from 19 Asiatic countries comprising 8 regional groups, which identify 2 distinct mitochondrial haplogroups, termed I1 and I2. The marked increase in nucleotide diversity (P < 0.001) for both the I1 and I2 haplogroups within the northern part of the Indian subcontinent is consistent with an origin for all domestic zebu in this area. For haplogroup I1, genetic diversity was highest within the Indus Valley among the three hypothesized domestication centers (Indus Valley, Ganges, and South India). These data support the Indus Valley as the most likely center of origin for the I1 haplogroup and a primary center of zebu domestication. However, for the I2 haplogroup, a complex pattern of diversity is detected, preventing the unambiguous pinpointing of the exact place of origin for this zebu maternal lineage. Our findings are discussed with respect to the archaeological record for zebu domestication within the Indian subcontinent.OXFORD UNIV PRESS, Jan. 2010, MOLECULAR BIOLOGY AND EVOLUTION, 27(1) (1), 1 - 6, English[Refereed]Scientific journal
- Genotype data from eight microsatellite markers were used to assess genetic diversity and relationships among five indigenous Bhutanese sheep populations, Sakten, Jakar, Sarpang, Sipsu and Tsirang. Estimates of mean observed and expected heterozygosities, mean number of alleles per locus/population were obtained. The highest observed heterozygosities were found in Jakar (0.657) and Sakten (0.647), while the lowest one was found in Tsirang (0.539). Genetic distances, pairwise proportion of different alleles, UPGMA tree, and principal component analysis indicate close relationship among Tsirang, Sipsu and Sarpang populations, while Jakar and Sakten populations are located in one cluster. These two clusters are separated geographically, and show distinct phenotypic as well as molecular characters. We therefore recommend that the Bhutanese native sheep populations be classified into at least two distinct breeds, Jakar-Sakten sheep and Sipsu sheep. Since Jakar and Sakten sheep have different morphological phenotypes, further analyses will be required to understand the genetic differences between these two sheep populations.WILEY-BLACKWELL PUBLISHING, INC, 2010, ANIMAL SCIENCE JOURNAL, 81(2) (2), 145 - 151, English[Refereed]Scientific journal
- This study describes the development of efficient single nucleotide polymorphism (SNP) markers for individual identification and parentage tests in a Japanese Black cattle population. An amplified fragment length polymorphism method was employed to detect informative candidate markers, and yielded 44 SNP markers from 220 primer combinations. 29 unlinked SNPs were finally selected as diagnostic markers. The allelic frequencies for each marker were estimated by using PCR-RFLP in the Japanese Black population. Based on the frequency data, the estimated identity power of these markers was 2.73 x 10-12. Parentage exclusion probabilities, when both suspected parents' genotypes were known and when only one suspected parent was genotyped, were estimated as 0.96929 and 0.99693, respectively. This panel of SNP markers is theoretically sufficient for individual identification, and would also be a powerful tool for a parentage test in Japanese Black cattle. The markers could contribute to the management of the beef industry in Japan.WILEY-BLACKWELL PUBLISHING, INC, 2010, ANIMAL SCIENCE JOURNAL, 81(2) (2), 152 - 157, English[Refereed]Scientific journal
- The purpose of this study was to assess genetic diversity, phylogenetic relationship and population structure among nine Eurasian cattle populations using 58 single nucleotide polymorphism (SNP) markers. The calculated distribution of minor allele frequencies and heterozygosities suggested that the genetic diversity of Bos indicus populations was lower than that of Bos taurus populations. Phylogenetic analyses revealed the main divergence between the Bos taurus and Bos indicus populations, and subsequently between Asian and European populations. By principal components analysis, the Bos taurus and Bos indicus populations were clearly distinguished with PC1 (61.1%); however, six Bos taurus populations clustered loosely and the partial separation between European and Asian groups was observed by PC2 (12.5%). The structure analysis was performed using the STRUCTURE program. Distinct separation between Bos taurus and Bos indicus was shown at K = 2, and that between European and Asian populations at K = 3. At K = 4, 5 and 6, Mongolian population showed an admixture pattern with different ancestry of Asian and European cattle. At K = 7, all Bos taurus populations showed each cluster with little proportion of admixture. In conclusion, 58 SNP markers in this study could sufficiently estimate the genetic diversity, relationship and structure for nine Eurasian cattle populations, especially by analyses of principal components and STRUCTURE.WILEY-BLACKWELL, 2010, ANIMAL SCIENCE JOURNAL, 81(3) (3), 281 - 289, English[Refereed]Scientific journal
- Individual identification and parentage analysis using DNA markers are essential for assuring food identity and managing livestock population. The objective of this study was to develop a single nucleotide polymorphism (SNP) panel system for individual effective identification and parentage testing in a Japanese Black cattle population using BovineSNP50 BeadChip. On the basis of SNP frequencies, 60 unlinked informative SNPs were finally selected as candidate markers. The allelic frequencies for each SNP were estimated using additional individuals by PCR-RFLP (restriction fragment length polymorphism). A total of 87 SNP markers added in conjunction with previously developed 27 SNPs were evaluated to assess the utility of the test. The estimated identity power was 2.01 x 10-34. Parentage exclusion probabilities, when both suspected parents' genotypes were known and when only one suspected parent was genotyped, were estimated as 0.99999997 and 0.99998010, respectively. This developed SNP panel was quite powerful and could successfully exclude false sires with a probability of > 0.9999 even if the dam's genotype information was not obtained. The SNP system would contribute to management of the beef industry in Japan.WILEY-BLACKWELL, 2010, ANIMAL SCIENCE JOURNAL, 81(4) (4), 506 - 512, English[Refereed]Scientific journal
- In the current study, milk protein variation was examined in cattle (Bos indicus), mithun (Bos frontalis), yak (Bos grunniens) and their hybrid populations in Bhutan to estimate genetic variability, conduct genetic characterization and assess the possibility of gene flow between mithun and cattle. Isoelectric focusing of 372 milk samples from 11 populations detected four molecular types of beta-lactoglobulin (A, B, E and M), five molecular types of alpha(S1) -casein (A, B, C, E and X) and three molecular types of k-casein (A, B and X). Mithun and yak shared alleles but were found to exhibit different allele frequencies for the proteins studied. The degree of genetic variability within populations was measured by average heterozygosity and ranged from 24-40% in cattle, 26% for yak and 33% for mithun. We also resolved the traditional mithun and cattle hybridization system via principal component analysis. Our results suggested secondary introgression of mithun genes to the village Thrabum population, and a close genetic relationship between Bhutanese indigenous cattle and Indian cattle.WILEY-BLACKWELL, 2010, ANIMAL SCIENCE JOURNAL, 81(5) (5), 523 - 529, English[Refereed]Scientific journal
- MC1R gene is known to be the main regulator of the switch between black and red coat color pigments in cattle. However, another gene would be associated with black and brown coat colors of cattle because our previous study reported that there were different coat color animals with the same MC1R genotype. The aim of this study was to identify the candidate region related to black/brown coat color in cattle. For this purpose, we genotyped 313 microsatellite markers evenly distributed across all cattle autosomes in the F2 Japanese Black × Limousin resource family. In addition, 36 microsatellite markers located within candidate regions were developed in order to narrow down the candidate region. Our results revealed that the responsible gene for black/brown coat color would be included in the region from 16.1Mb to 26.5Mb on BTA 16.Japanese Society of Animal Genetics, Jun. 2009, Journal of Animal Genetics, 37(1) (1), 3 - 8, English[Refereed]Scientific journal
- MC1R gene is known to be the main regulator of the switch between black and red coat color pigments in cattle. However, another gene would be associated with black and brown coat colors of cattle because our previous study reported that there were different coat color animals with the same MC1R genotype. The aim of this study was to identify the candidate region related to black/brown coat color in cattle. For this purpose, we genotyped 313 microsatellite markers evenly distributed across all cattle autosomes in the F2 Japanese Black × Limousin resource family. In addition, 36 microsatellite markers located within candidate regions were developed in order to narrow down the candidate region. Our results revealed that the responsible gene for black/brown coat color would be included in the region from 16.1Mb to 26.5Mb on BTA 16.Japanese Society of Animal Genetics, Jun. 2009, Journal of Animal Genetics, 37(1) (1), 3 - 8, English[Refereed]Scientific journal
- Fatty acid composition of beef adipose tissue is one of important traits because high proportions of monounsaturated fatty acid are related to favorable beef flavor and tenderness. In this study, we investigated effects of genetic factors such as stearoyl-CoA desaturase (SCD) and sterol regulatory element binding protein (SREBP) on beef carcass traits including fatty acid composition using two cattle populations. Sire effect was significantly related to almost all traits except BMS, suggesting that the trait examined in this study is highly controlled by genetic factors. The effect of SCD genotype on fatty acid composition was detected remarkably in both cattle groups, especially on stearic and oleic acids. This result was consistent with our previous studies and suggests that SCD is associated with fatty acid composition. Unlike SCD genotyping, the effect of SREBP genotype was not identified in this study. Our results suggested that SCD genotype would contribute to improving beef quality in field populations. Further studies about the relationship among these factors will bring an insight into the molecular mechanism of fatty acid metabolism in cattle.WILEY-BLACKWELL PUBLISHING, INC, Jun. 2009, ANIMAL SCIENCE JOURNAL, 80(3) (3), 225 - 232, English[Refereed]Scientific journal
- The WW domain containing E3 ubiquitin protein ligase 1 (WWP1) is classified into one of ubiquitin ligases which play an important role in ubiquitin-proteasome pathway. Previously, we identified the WWP1 gene as a candidate gene of chicken Muscular dystrophy by linkage analysis and sequence comparison. However, the mechanism causing pathological changes and underlaying gene function remains elucidated. In the present study. we analyzed the WWP1 gene expression in various Muscles and tissues of normal chickens, and compared with those Front muscular dystrophic chickens. Two mRNA isoforms were detected in all tissues examined and revealed almost equal expression level. The WWP1 expression of dystrophic chickens was decreased in almost all skeletal Muscles including unaffected Muscles. These data indicate that there might not be a causal relationship between the alteration of WWP1 expression level and the severity of muscular dystrophy.JAPAN POULTRY SCIENCE ASSOC, Apr. 2009, JOURNAL OF POULTRY SCIENCE, 46(2) (2), 95 - 99, English[Refereed]Scientific journal
- Growth hormone receptor (GHR) belongs to a member of the cytokine receptor superfamily. Polymorphism of presence or absence of an approximately 1.2 kbp LINE-1 element is observed in bovine GHR gene. The present study was carried out for estimating the genetic diversity and the origin of the LINE-1 element in 10 European, Southeastern Asian and East Asian cattle breeds or populations. Genotyping of the LINE-1 revealed predominant LINE-1 presence in European breeds (0.917 similar to 0.991), absence in the Bos taurus indicus populations (0.000 similar to 0.017), and intermediate presence in Northeast Asian cattle (0.417 similar to 0.522). From genetic features of LINE families, LINE-1 of GHR could be attributed to the same origin in both European and Asian cattle, and Asian LINE-1 may not be derived from recent introgression. This result suggested that LINE-1 in bovine GHR gene could have arisen in an ancestral population of Bos taurus taurus.WILEY, 2009, ANIMAL SCIENCE JOURNAL, 80(5) (5), 528 - 531, English[Refereed]Scientific journal
- Background: Fatty acid composition has become an important trait in the beef industry in terms of beef flavor and decreasing the circulating concentration of LDL cholesterol. In this study, we examined the association between polymorphisms of six genes, adipocytes-type fatty acid binding protein (FABP4), liver X receptor alpha (LXR alpha), cytochrome b(5) (Cyt b(5)), long-chain acyl-CoA synthetase (ACSL) 1, ACSL4 and diacylglycerol acyltransferase 2 (DGAT2) and fatty acid composition. Results: Sequence comparisons revealed 14 single nucleotide polymorphisms in six genes. Four of them, I74V and V110M in FABP4 and G51E and V133I in LXR alpha, were nonsynonymous substitutions. The associations between the genotypes and fatty acid compositions were analyzed by using 234 Japanese Black cattle. The genotypes of FABP4 I74V and LXR alpha V133I were significantly associated with palmitoleic acids (C16: 1, P = 0.0086) and linoleic acid (C18:2, P = 0.0121) content in intramuscular fat, respectively. Conclusion: Our findings suggest that the two polymorphisms of FABP4 I74V and LXR alpha V133I might be genetic factors in part associated with palmitoleic acid (FABP4 I74V) and linoleic acid (LXR alpha V133I) composition in intramuscular fat of Japanese Black cattle, respectively. Especially, FABP4 I74V had highly significant effect (P < 0.01) on C16:1 proportion, indicating that the I/I homozygote exhibited 0.5% higher percentage than V/V homozygote.BIOMED CENTRAL LTD, Dec. 2008, BMC GENETICS, 9, 84, English[Refereed]Scientific journal
- This study seeks to investigate the genetic variability of PRNP in Asian goats. We sequenced the PRNP coding region using a total of 193 samples from seven Asian countries (Japan, Laos, Vietnam. Bhutan, Mongolia. Myanmar and Cambodia). Sequence comparison revealed five previously reported polymorphisms in the PRNP coding region. Two of those polymorphisms (G126A and C414T) were silent mutations, and the other three (T304G, A428G and T718C) Caused amino acid changes (W102G, H143R and S240P). In the total of 193 animals, one amino acid mutation (T304G) exhibited low variability (minor allele frequency = 0.04), but the other four were high (0.31-0.36). In addition, allele frequencies of C414T and T718C exhibited remarkable differences among countries (p-values of 6.50E-17 and 5.49E-18). These results Suggest high genetic variability of PRNP among these countries and are useful information for estimating genetic diversity in Asian goats. (C) 2008 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, Nov. 2008, SMALL RUMINANT RESEARCH, 80(1-3) (1-3), 101 - 103, English[Refereed]Scientific journal
- In this study, we analyzed DNA sequence of mitochondrial DNA (mtDNA) control regions on the 130 native domestic pigs and eight wild boars in the mainland South and South-east Asian countries including Bhutan, Cambodia, Laos, Myanmar, and Vietnam. Forty-four haplotypes were found in the 138 individuals, 41 were in the domestic and four were in wild boars. Only one haplotype was shared by domestic and wild population in Bhutan. In other cases, mtDNA of wild boars did not show close affinity to that of the domestic pigs in the same location, indicating that the native domestic pigs in these countries did not originate in the present habitat. Phylogenetic analyzes of mtDNA haplotypes recapitulated several major clusters identified in other studies, but 11 haplotypes were grouped in a new cluster we named MTSEA. In most cases, more than one lineage group were present in a sampling station, indicating that the present indigenous domestic pigs may have multiple origins. The MTSEA haplotypes were present in relatively high frequencies in domestic pigs in the mountainous area of mainland South-east Asia (Cambodia and Laos), with a few found in Myanmar and Bhutan. The distributions of MTSEA haplotypes are in great conformity with the distribution of present-day Mon-Khmer language and indicated the existence of yet another independent domestication. The D2 haplotypes that distribute high frequency (almost 100%) throughout the Chinese breeds were dominant in Bhutan, Myanmar, and Vietnam. These results suggest an existence of human-mediated dispersal of domestic pigs from north to the south during the historical expansion of Sino-Tibetan and Tai peoples. The D3 haplotypes previously reported in north India were found in sympatric domestic and wild pigs in Bhutan. The D3 haplotype is an important proof of independent domestication event and/or great gene flow between wild and domestic pigs in the foot of Himalaya.WILEY-BLACKWELL, Aug. 2008, ANIMAL SCIENCE JOURNAL, 79(4) (4), 417 - 434, English[Refereed]Scientific journal
- Chicken muscular dystrophy with abnormal muscle (AM) has been studied for more than 50 years, but the gene responsible for it remains unclear. Our previous studies narrowed down the AM candidate region to approximately 1 Mbp of chicken chromosome 2q containing seven genes. In this study, we performed sequence comparison and gene expression analysis to elucidate the responsible gene. One missense mutation was detected in AM candidate genes, while no remarkable alteration of expression patterns was observed. The mutation was identified in WWP1, detected only in dystrophic chickens within several tetrapods. These results suggested WWP1 is responsible for chicken muscular dystrophy. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, Jun. 2008, FEBS LETTERS, 582(15) (15), 2212 - 2218, English[Refereed]Scientific journal
- Background: Analyses of population structure and breed diversity have provided insight into the origin and evolution of cattle. Previously, these studies have used a low density of microsatellite markers, however, with the large number of single nucleotide polymorphism markers that are now available, it is possible to perform genome wide population genetic analyses in cattle. In this study, we used a high-density panel of SNP markers to examine population structure and diversity among eight cattle breeds sampled from Bos indicus and Bos taurus. Results: Two thousand six hundred and forty one single nucleotide polymorphisms ( SNPs) spanning all of the bovine autosomal genome were genotyped in Angus, Brahman, Charolais, Dutch Black and White Dairy, Holstein, Japanese Black, Limousin and Nelore cattle. Population structure was examined using the linkage model in the program STRUCTURE and Fst estimates were used to construct a neighbor-joining tree to represent the phylogenetic relationship among these breeds. Conclusion: The whole-genome SNP panel identified several levels of population substructure in the set of examined cattle breeds. The greatest level of genetic differentiation was detected between the Bos taurus and Bos indicus breeds. When the Bos indicus breeds were excluded from the analysis, genetic differences among beef versus dairy and European versus Asian breeds were detected among the Bos taurus breeds. Exploration of the number of SNP loci required to differentiate between breeds showed that for 100 SNP loci, individuals could only be correctly clustered into breeds 50% of the time, thus a large number of SNP markers are required to replace the 30 microsatellite markers that are currently commonly used in genetic diversity studies.BIOMED CENTRAL LTD, May 2008, BMC GENETICS, 9, 37, English[Refereed]Scientific journal
- Discrimination of Korean cattle (Hanwoo) using DNA markers derived from SNPs in bovine mitochondrial and SRY genesIn order to distinguish Korean cattle (Hanwoo) beef from the imported beef from Australia in Korean markets, DNA markers based on PCR-RFLP from mitochondrial genes and SRY gene were applied. A total of 2,826 beef samples comprising 1,495 Hanwoo and 1,331 foreign cattle breeds were obtained in Korea. An 801 bp fragment of the SRY gene on the bovine Y chromosome, a 343 bp fragment of ND4 gene and a 528 bp fragment of ND5 gene in the bovine mtDNA were amplified by PCR and digested with three restriction enzymes, Msel, HpyCH(4)III and Tsp509I, respectively. The results showed that Bos taurus (T) type was the majority in Hanwoo by combining three markers (99.5%). However, 78.2% of Bos indicus (1) type was observed in the imported beef samples. These results indicated that three markers used in this study will be used as valuable markers for discriminating imported beef against Hanwoo.ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC, Jan. 2008, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 21(1) (1), 25 - 28, English[Refereed]Scientific journal
- In this study, we describe intraspecies variation in the alpha C connector region of the bovine fibrinogen A alpha gene. Sequencing and genotyping of six bovine breeds revealed 7 to 10 tandem repeats in the alpha C connector region. In addition, we observed length differences between B. indicus and B. taurus, with the B. indicus having longer fibrinogen alpha C connectors (10-repeat alleles) than B. taurus (7- and 9-repeats). The difference in tandem repeats may be related to the function of blood coagulation system.TAYLOR & FRANCIS INC, 2008, ANIMAL BIOTECHNOLOGY, 19(2) (2), 122 - 126, English[Refereed]Scientific journal
- To investigate genetic factors that affect fatty acid composition in beef carcass, we previously investigated genetic profiles of stearoyl-CoA desaturase (SCD) and their effect on fatty acid composition in fat tissue of cattle. It has been known that sterol regulatory element binding protein (SREBP) is a transcription factor that regulates gene expression levels of SCD and other genes relevant to lipid and fatty acid metabolism in tissue. Therefore, we determined the full-length sequence of bovine SREBP-1 cDNA and then surveyed polymorphisms in whole exons and introns in the bovine genome. Large 84-bp insertion (long type: L) and deletion (short type: S) were found in intron 5 of bovine SREBP-1 in Japanese Black cattle, although there was no notable mutation in exon regions. The associations between the SREBP-1 genotypes and fatty acid compositions/fat melting points were analyzed by using genomic DNA with carcass trait information from 606 Japanese Black cattle. The S type contributed to 1.3% higher monounsaturated fatty acid (MUFA) proportion and 1.6 degrees C lower melting point in intramuscular fat. Genotyping of bovine SREBP-1 is considered to reflect a genetic variation which is associated with physiologic characteristics of fat tissue in Japanese black cattle.SPRINGER, Dec. 2007, MAMMALIAN GENOME, 18(12) (12), 880 - 886, English[Refereed]Scientific journal
- Stearoyl-CoA desaturase (SCD) catalyzes the synthesis of monounsaturated fatty acids (MUFAs). In cattle, the MUFAs are related to softness and flavor of meat. In order to investigate gene expression profile during bovine preadipocyte differentiation, we isolated stromal-vascular cells from perirenal adipose tissues of Japanese Black and Holstein steers. Gene expression level of adipocyte type fatty acid binding protein (FABP4), SCD, sterol regulatory element binding protein 1 (SREBP1) and CCAAT/enhancer binding protein alpha (C/EBP-alpha) were elucidated by real-time PCR assay. The levels of SCD mRNA expression were significantly increased to 10.8 and 6.3-fold in Japanese Black and Holstein, respectively, on day 1 of the culture. The difference in SCD expression between the two breeds may reflect differences in the fat development characteristics of the cattle breeds. Although transcription factors SREBP1 and C/EBP-alpha are supposed to regulate SCD expression, expression levels of the two factors were not completely consistent with that of SCD. (c) 2007 Elsevier Inc. All rights reserved.ELSEVIER SCIENCE INC, Nov. 2007, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 148(3) (3), 629 - 634, English[Refereed]Scientific journal
- Background: Bovine whole genome linkage disequilibrium maps were constructed for eight breeds of cattle. These data provide fundamental information concerning bovine genome organization which will allow the design of studies to associate genetic variation with economically important traits and also provides background information concerning the extent of long range linkage disequilibrium in cattle. Results: Linkage disequilibrium was assessed using r(2) among all pairs of syntenic markers within eight breeds of cattle from the Bos taurus and Bos indicus subspecies. Bos taurus breeds included Angus, Charolais, Dutch Black and White Dairy, Holstein, Japanese Black and Limousin while Bos indicus breeds included Brahman and Nelore. Approximately 2670 markers spanning the entire bovine autosomal genome were used to estimate pairwise r(2) values. We found that the extent of linkage disequilibrium is no more than 0.5 Mb in these eight breeds of cattle. Conclusion: Linkage disequilibrium in cattle has previously been reported to extend several tens of centimorgans. Our results, based on a much larger sample of marker loci and across eight breeds of cattle indicate that in cattle linkage disequilibrium persists over much more limited distances. Our findings suggest that 30,000 - 50,000 loci will be needed to conduct whole genome association studies in cattle.BIOMED CENTRAL LTD, Oct. 2007, BMC GENETICS, 8, 74, English[Refereed]Scientific journal
- Muscular dystrophies, a group of inherited diseases with the progressive weakness and degeneration of skeletal muscle, contain genetically variable diseases. Though chicken muscular dystrophy with abnormal muscle (AM) has long been known, the gene responsible has not yet been identified. In this study, a resource family for AM was established with 487 F2 individuals and 22 gene markers, including microsatellite and insertion-deletion markers, were developed. The haplotypes were analyzed with these markers for the candidate region of GGA2q described in a previous study. The candidate region was successfully narrowed down to approximately 1Mbp. The region included seven functional genes predicted as the most likely AM candidates.BLACKWELL PUBLISHING, Oct. 2007, ANIMAL SCIENCE JOURNAL, 78(5) (5), 476 - 483, English[Refereed]Scientific journal
- In the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. This paper describes the development of DNA markers to discriminate between Japanese and Australian beef. Two Bos indicus-specific markers and MCIR marker were used as possible candidate markers. Amplified fragment length polymorphism method was employed to develop additional candidate markers. The 1564 primer combinations provided three markers that were converted into single nucleotide polymorphisms markers for high-throughput genotyping. In these markers, the allele frequencies in cattle from both countries were investigated for discrimination ability using PCR-RFLP. The probability of identifying Australian beef was 0.933 and probability of misjudgment was 0.017 using six selected markers. These markers could be useful for discriminating between Japanese and Australian beef and would contribute to the prevention of falsified breed labeling of meat. (c) 2007 Elsevier Ltd. All rights reserved.ELSEVIER SCI LTD, Oct. 2007, MEAT SCIENCE, 77(2) (2), 161 - 166, English[Refereed]Scientific journal
- The beta 3-adrenergic receptor (ADRB3) is a G protein-coupled receptor that is involved in regulating energy homeostasis. We have studied DNA sequences of porcine ADRB3 to find candidate genetic polymorphisms for economically important growth and performance traits in pigs. Five novel haplotypes derived from the three In/dels and 44 SNPs were identified among domestic pigs and wild boars. Three of them encode non-synonymous amino acid sequences by five missense polymorphisms and a frameshift by a thymine insertion. The amino acid polymorphic sites were distributed as follows: one substitution was in extracellular loop 1, three substitutions were in intracellular loop3 and one substitution and the deletion of two amino acids were at the carboxyl-terminal. There was no polymorphism in the transmembrane domains. In addition, we surveyed the allelic frequency of the thymine insertion that cause frameshift in South-east Asian local pigs, including some commercial breeds and wild boars. This thymine insertion was distributed widely in the domestic pigs and wild boars. The frequencies of this allele were relatively low in Western improved breeds, while they were very common in Asian breeds and wild boars in Asia. This result indicates that this insertion originally occurred in ancient Asian wild boars and then circulated among Asian domestic pigs. This allele also spread over Western breeds, probably through the introgression of Asian pigs into European stocks during the 18th and 19th centuries.WILEY-BLACKWELL, Jun. 2007, ANIMAL SCIENCE JOURNAL, 78(3) (3), 243 - 250, English[Refereed]Scientific journal
- Proteomic comparison between Japanese Black and Holstein cattle by two-dimensional gel electrophoresis and identification of proteinsDifferences of meat qualities between Japanese Black and Holstein have been known in Japan, however, the causative proteins and/or the genetic background have been unclear. The aim of this study was to identify candidate proteins causing differences of the meat qualities between the two breeds. Using technique of two-dimensional gel electrophoresis, protein profiling was compared from samples of the longissimus dorsi muscle and subcutaneous adipose tissue. Five protein spots were observed with different expression levels between breeds. By using LC-MS/MS analysis and Mascot program, three of them were identified as ankyrin repeat protein 2, phosphoylated myosin light chain 2 and mimecan protein. Subsequently, we compared the DNA coding sequences of three proteins between breeds to find any nucleotide substitution. However, there was no notable mutation which could affect pI or molecular mass of the proteins. The identified proteins may be responsible for different characteristics of the meat qualities between Japanese Black and Holstein cattle.ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOCIETIES, May 2007, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 20(5) (5), 638 - 644, English[Refereed]Scientific journal
- Bov-A2 is a retroposon that is widely distributed among the genomes of ruminants (e.g., cow, deer, giraffe, pronghorn, musk deer, and chevrotain). This retroposon is composed of two monomers, called Bov-A units, which are joined by a linker sequence. The structure and origin of Bov-A2 has been well characterized but a genome-level exploration of this retroposon has not been implemented. In this study we performed an extensive search for Bov-A2 using all available genome sequence data on Bos taurus. We found unique Bov-A2-derived sequences that were longer than Bov-A2 due to amplification of three to six Bov-A units arranged in tandem. Detailed analysis of these elongated Bov-A2-derived sequences revealed that they originated through unequal crossing-over of Bov-A2. We found a large number of these elongated Bov-A2-derived sequences in cattle genomes, indicating that unequal crossing-over of Bov-A2 occurred very frequently. We found that this type of elongation is not observed in wild bovine and is therefore specific to the domesticated cattle genome. Furthermore, at specific loci, the number of Bov-A units was also polymorphic between alleles, implying that the elongation of Bov-A units might have occurred very recently. For these reasons, we speculate that genomic instability in bovine genomes can lead to extensive unequal crossing-over of Bov-A2 and levels of polymorphism might be generated in part by repeated outbreeding.SPRINGER, Mar. 2007, MAMMALIAN GENOME, 18(3) (3), 187 - 196, English[Refereed]Scientific journal
- Springer, 2007, Vertebrate Paleobiology and Paleoanthropology, (9781402055614) (9781402055614), 385 - 391, EnglishIn book
- Japanese Society of Animal Breeding and Genetics, 2007, Journal of Animal Genetics, 35(1):5-10(1) (1), 5 - 10, English[Refereed]Scientific journal
- ここ数年来、牛肉品種の偽装問題により、牛肉に対する消費者の信頼は落ち、牛肉の需要にも影響している。偽装表示を防ぐための牛肉の鑑別手法の開発には、大きく2種類の方向性がある。一つは、国内産牛肉内での識別であり、これは黒毛和種と交雑牛 (F1) を含むその他品種間での識別である。二つ目としては、輸入牛肉と国内産牛肉間での識別である。我々は牛肉の偽装表示を防ぐ目的で、DNAマーカーを用いた品種識別法の開発に取り組んできた。その結果、1) 国内産牛の黒毛和種、ホルスタイン種、およびそのF1を識別するDNAマーカーの開発を行い、品種識別に有効な11のマーカーを開発した。特に4マーカーが有効であり、F1の検出率が91.7%、信頼度が99.3%であった。2) 豪州産輸入牛肉と国産牛肉を識別するマーカーの開発に取り組んだ。現在までにmtDNA、Y染色体、核ゲノム由来からなる6つのマーカーを開発した。このシステムによる豪州産輸入牛肉の検出頻度は92.1%、信頼度は98.5%である。この開発により、国産牛内と豪州産輸入牛肉のDNA識別が可能となった。日本動物遺伝育種学会, Nov. 2006, 動物遺伝育種研究 = The journal of animal genetics, 34, 1 - 12, Japanese
- Mitochondrial DNA variation and genetic relationships in Japanese and Korean cattleThe complete mtDNA D-loop regions of Japanese and Korean cattle were analyzed for their mtDNA variations and genetic relationships. Sequencing the 30 Higo substrain and 30 Tosa substrain of Japanese Brown, respectively 12 and 17 distinct Bos haplotypes were identified from 77 polymorphic nucleotide sites. In order to focus on the relationships among Japanese and Korean cattle, two types of phylogenetic tree were constructed using individual sequences; first, a neighbor-joining tree with all sequences and second, reduced median networks within each Japanese and Korean cattle group. The trees revealed that two major mtDNA haplotype groups, T3 and T4, were represented in Japanese and Korean cattle. The T4 haplogroup predominated in Japanese Black and Japanese Brown cattle (frequency of 43.3-66.7%), while the T3 haplogroup was predominant (83.3%) and T4 was represented only twice in the Korean cattle. The results suggested that the mitochondrial origins of Japanese Brown were Japanese ancient cattle as well as Japanese Black in despite of the considerable introgression of Korean and European cattle into Japanese Brown.ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOCIETIES, Oct. 2006, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 19(10) (10), 1394 - 1398, English[Refereed]Scientific journal
- Breed discrimination using DNA markers derived from AFLP in Japanese beef cattleIn the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. This paper describes the development of DNA markers to discriminate between Japanese Black and F1 (Japanese Black x Holstein) breeds. The amplified fragment length polymorphism method was employed to detect candidate markers absent in Japanese Black but present in Holstein. The 1,754 primer combinations yielded eleven markers that were converted into single nucleotide polymorphism markers for high-throughput genotyping. The allele frequencies in both breeds were investigated for discrimination ability using PCR-RFLP. The probability of identifying F1 was 0.9168 and probability of misjudgment was 0.0066 using four selected markers. The markers could be useful for discriminating between Japanese Black and F1 and would contribute to the prevention of falsified breed labeling of meat.ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOCIETIES, Aug. 2006, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 19(8) (8), 1106 - 1110, English[Refereed]Scientific journal
- In order to develop a comparative map between chicken and quail, we identified orthologous gene markers based on chicken genomic sequences and localized them on the Japanese quail Kobe-NIBS linkage map, which had previously been constructed with amplified fragment length polymorphisms. After sequencing the intronic regions of 168 genes located on chicken chromosomes 1-8, polymorphisms among Kobe-NIBS quail family parents were detected in 51 genes. These orthologous markers were mapped on eight Japanese quail linkage groups (JQG), and they allowed the comparison of JQG to chicken macrochromosomes. The locations of the genes and their orders were quite similar between the two species except within a previously reported inversion on quail chromosome 2. Therefore, we propose that the respective quail linkage groups are macrochromosomes and designated as quail chromosomes CJA 1-8.BLACKWELL PUBLISHING, Aug. 2006, ANIMAL GENETICS, 37(4) (4), 316 - 320, English[Refereed]Scientific journal
- Mitochondrial DNA diversity of Korean native goatsKorean native goats have lived on the Korean peninsula for more than 2,000 Nears and are regarded as a valuable genetic resource for the world. As an initial step to investigate the genetic structures of this breed, phylogenetic analysis and calculation of genetic diversities have been performed using mitochondrial DNA (mtDNA) sequence variations. A total of 19 Korean native goats were grouped into six haplotypes and the large majority of haplotypes were present in 13 animals, All mtDNA of these Korean goat; belonged to the mitochondrial (mt) lineage A and revealed remarkably small genetic distances within the Population when compared with other Asian goat populations, indicating less genetic variation in the Korean native goats. These results indicate high-inbred status of the Korean native goats and will influence breeding and conservation strategies adopted for this breed.ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC, Apr. 2006, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 19(4) (4), 482 - 485, English[Refereed]Scientific journal
- In our previous study, a Kobe-NIBS Japanese quail (KNQ) linkage map was constructed mainly using amplified fragment length polymorphism (AFLP) markers. In order to compare chicken and quail chromosomes, we developed expressed sequence tag (EST) markers derived from cDNA-AFLP fragments and localized these markers on the linkage map, Using a total of 128 AFLP primer combinations, 24 polymorphic bands were obtained between a neurofilament-deficient mutant quail line male and a muscular disorder quail line female, which were the parents of the KNQ resource family. Nine of the 24 markers were mapped by linkage analysis. These markers were mapped to seven linkage groups, namely 1, 2, 3, 6, 8, 15 and 42. A subsequent homology search using chicken genome sequences strongly suggests that these linkage groups correspond with chicken chromosomes 1, 2, 3, 5, 15, 23 and 26.BLACKWELL PUBLISHING, Feb. 2006, ANIMAL SCIENCE JOURNAL, 77(1) (1), 42 - 46, English[Refereed]Scientific journal
- Japanese Society of Animal Breeding and Genetics, 2006, The journal of animal genetics, 34(1) (1), 41 - 45
- The Japanese quail (Coturnix japonica) is a notably valuable egg and meat producer but has also been used as a laboratory animal. In the present study, we constructed a Japanese quail linkage map with 1735 polymorphic amplified fragment length polymorphisms markers, and nine chicken microsatellite (MS) markers, as well as sex and phenotypes of two genetic diseases; a muscular disorder (LWC) and neurofilament-deficient mutant (Quv). Linkage analysis revealed 578 independent loci. The resulting linkage map contained 44 multipoint linkage groups covering 2597.8 cM and an additional 218.2 cM was contained in 21 two-point linkage groups. The total map was 2816 cM in length with an average marker interval of 5.5 cM. The Quv locus was located on linkage group 5, but linkage was not found between the LWC locus and any of the markers. Comparative mapping with chicken using orthologous markers revealed chromosomal assignments of the quail linkage group 1 to chicken chromosome 2 (GGA2), 5 to GGA22, 2 to GGA5, 8 to GGA7, 27 to GGA11, 29 to GGA1 and 45 to GGA4.BLACKWELL PUBLISHING, Jun. 2005, ANIMAL GENETICS, 36(3) (3), 227 - 231, EnglishScientific journal
- In order to estimate the influence of the Extension (E) locus in cattle coat color, the melanocortin-1 receptor (MC1R) gene in Japanese Black, Japanese Brown and Korean (Hanwoo) cattle were sequenced. The sequences of the coding region revealed three alleles (ED, E+ and e), which were previously reported. Polymerase chain reaction-restriction fragment length polymorphism was performed to investigate the gene frequencies of the three breeds. Japanese Black was almost composed of ED and E+ individuals, ED = 0.481 and E+ = 0.514, and no homozygous e/e, therefore that is consistent with the hypothesis that ED and E+ induce black pigment synthesis. Allele frequencies between Japanese Brown and Hanwoo were obviously different however, recessive red e allele frequency was 0.038 for Japanese Brown and 0.948 for Hanwoo, even though both breeds have quite similar coat colors (ranging from yellowish brown to dark brown including a red coat color). This result suggested that other genes are also associated with a coat color of red and brown in cattle.Apr. 2005, Animal Science Journal, 76(2) (2), 129 - 132, EnglishScientific journal
- We used a 9.6 K cattle muscle/fat cDNA microarray to study gene expression differences between the longuissimus dorsi (LD) muscle of Japanese Black (JB) and Holstein (HOL) cattle. JB cattle exhibit an unusual ability to accumulate intramuscular adipose tissue with fat melting points lower than that in other breeds. The LD biopsies from three JB (Tajima strain) and three HOL animals were used in this breed comparison. Seventeen genes were identified as preferentially expressed in LD samples from JB and seven genes were found to be expressed more highly in HOL. The expression of six selected differentially expressed genes was confirmed by quantitative real-time PCR. The genes more highly expressed in JB are associated with unsaturated fatty acid synthesis, fat deposition, and the thyroid hormone pathway. These results are consistent with the increased amounts and proportions of monounsaturated fatty acids observed in the muscle of JB animals. By discovering as yet uncharacterized genes that are differentially regulated in this comparison, the work may lead us to a better understanding of the regulatory pathways involved in the development of intramuscular adipose tissue.SPRINGER, Mar. 2005, MAMMALIAN GENOME, 16(3) (3), 201 - 210, English[Refereed]Scientific journal
- We used a 9.6 K cattle muscle/fat cDNA microarray to study gene expression differences between the longuissimus dorsi (LD) muscle of Japanese Black (JB) and Holstein (HOL) cattle. JB cattle exhibit an unusual ability to accumulate intramuscular adipose tissue with fat melting points lower than that in other breeds. The LD biopsies from three JB (Tajima strain) and three HOL animals were used in this breed comparison. Seventeen genes were identified as preferentially expressed in LD samples from JB and seven genes were found to be expressed more highly in HOL. The expression of six selected differentially expressed genes was confirmed by quantitative real-time PCR. The genes more highly expressed in JB are associated with unsaturated fatty acid synthesis, fat deposition, and the thyroid hormone pathway. These results are consistent with the increased amounts and proportions of monounsaturated fatty acids observed in the muscle of JB animals. By discovering as yet uncharacterized genes that are differentially regulated in this comparison, the work may lead us to a better understanding of the regulatory pathways involved in the development of intramuscular adipose tissue.SPRINGER, Mar. 2005, MAMMALIAN GENOME, 16(3) (3), 201 - 210, EnglishScientific journal
- Japanese Black cattle are characterised by a unique ability to deposit intramuscular fat with lower melting temperature. In this study, 3 consecutive biopsies from Longissimus muscle tissue were taken and RNA isolated from 3 Japanese Black (Tajima strain) and 3 Holstein animals at age 11 - 20 months. The gene expression changes in these samples were analysed using a bovine fat/muscle cDNA microarray. A mixed-ANOVA model was fitted to the intensity signals. A total of 335 (4.8%) array elements were identified as differentially expressed genes in this breed x time comparison study. Genes preferentially expressed in Japanese Black are associated with mono-unsaturated fatty acid synthesis, fat deposition, adipogenesis development and muscle regulation, while examples of genes preferentially expressed in Holstein come from functional classes involved in connective tissue and skeletal muscle development. The gene expression differences detected between the Longissimus muscle of the 2 breeds give important clues to the molecular basis for the unique features of the Japanese Black breed, such as the onset and rate of adipose tissue development, metabolic differences, and signalling pathways involved in converting carbohydrate to lipid during lipogenesis. These findings will impact on industry management strategies designed to manipulate intramuscular adipose development at different development stages to gain maximum return for beef products.C S I R O PUBLISHING, 2005, AUSTRALIAN JOURNAL OF EXPERIMENTAL AGRICULTURE, 45(7-8) (7-8), 809 - 820, EnglishScientific journal
- Japan Poultry Science Association, 2005, J. Poult. Sci., 42:263-271(3) (3), 263 - 271, EnglishDevelopment and Mapping of Microsatellite Markers derived from cDNA in Japanese Quail (Coturnix japonica)Scientific journal
- Genealogical relationship between pedigree and microsatellite information and analysis of genetic structure of a highly inbred Japanese black cattle strainJapanese Black cattle of Hyogo prefecture (Tajima strain) are famous for its ability to produce high-quality meat and have been maintained as a closed system for more than 80 years. In order to assess the usefulness of microsatellite markers in closed cattle populations, and evaluate the genetic structure of the Tajima strain, we analyzed representative dams of the Tajima strain comprised of the substrains Nakadoi and Kinosaki. Genetic variability analyses indicated-low genetic diversity in the Tajima strain. In addition, a recent genetic bottleneck, which could be accounted for by the high level of inbreeding, was detected in both substrains. In phylogenetic analyses, relationship coefficients and genetic distances between individuals were calculated using pedigree and microsatellite information. Two phylogenetic trees were constructed from microsatellite and pedigree information using the UPGMA method. Both trees illustrated that most individuals were distinguished clearly on the basis of the two substrains, although in the microsatellite tree some individuals appeared in clusters of different substrains. Comparing the two phylogenetic trees revealed good consistency between the microsatellite analysis tree and the pedigree information. The correlation coefficient between genetic distances derived from microsatellite and pedigree information was 0.686 with a high significance level (p<0.001). These results indicated that microsatellite information may provide data substantially equivalent to pedigree information even in unusually inbred herds of cattle, and suggested that microsatellite markers may be useful in revealing genetic structure without accurate or complete pedigree information.ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOCIETIES, Oct. 2004, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 17(10) (10), 1355 - 1359, EnglishScientific journal
- Our previous studies revealed that the genetic locus for chicken muscular dystrophy of abnormal muscle (AM) mapped to chromosome 2q, and that the region showed conserved synteny with human chromosome 8q11-24.3. In the current study, we mapped the chicken orthologues of genes from human chromosome 8q11-24 in order to identify the responsible gene. Polymorphisms in the chicken orthologues were identified in the parents of the resource family. Twenty-three genes and expressed sequence tags (ESTs) were mapped to chicken chromosome 2 by linkage analysis. The detailed comparative map shows a high conservation of synteny between chicken chromosome 2q and human chromosome 8q. The AM locus was mapped between [inositol(myo)-1(or4)-monophosphatase 1] (IMPA1) gene and [core-binding factor, runt domain, alpha-subunit 2; translocated to 1; cyclin D-related] (CBFA2T1) gene. The genes located between IMPA1 and CBFA2T1 are the most likely candidates for chicken muscular dystrophy.BLACKWELL PUBLISHING LTD, Oct. 2004, ANIMAL GENETICS, 35(5) (5), 397 - 400, EnglishScientific journal
- Trace of native cattle in Japanese Holstein assessed by mitochondrial DNA sequence polymorphismOn the basis of sequence variation in the displacement loop region of mtDNA, 588 Japanese and North American Holstein cows were classified into 5 mitochondrial haplotypes, which were found in Japanese Black cattle. One of the haplotypes (named type 1), which was present at the highest frequency in Japanese Black cattle, was not observed in either European or African cattle. This haplotype is characterized by 2 single-nucleotide polymorphisms. One is called the type B polymorphism, and it refers to a base change from T to C at nucleotide 16042 of the mitochondrial genome (T160042C). The other is called the type I polymorphism, and it refers to the base change as G16093A. The proportion of the Japanese Holstein population with both polymorphisms was 18.3%, whereas none of the North American cows had this genotype. Because the mitochondrial types were inherited maternally, it is clear that a considerable number of Japanese Holstein cows are descended from native Japanese cattle. Polymorphisms B and I accounted for no variance in the estimated breeding value for milk production among cows from the Hyogo herd (582 cows) or the Chiba region herd (758 cows). This result suggests that most autosomal genes of native animals have been successively replaced by those of pure Holstein after grading up of over 15 generations, even though resulting animals have native animal-oriented mitochondrial types and may still have some number of the native autosomal genes.AMER DAIRY SCIENCE ASSOC, Sep. 2004, JOURNAL OF DAIRY SCIENCE, 87(9) (9), 3071 - 3075, EnglishScientific journal
- Complete mitochondrial DNA (mtDNA) control region of 30 Pakistani domestic goats was sequenced to investigate the genetic diversity and organization. Twenty-two new haplotypes were observed and all were classified into mt-lineage A. Phylogenetic analysis revealed two distinct clusters in mt-lineage A, A1 and A2. A 17 bp deletion and a 76 bp insertion were detected in the L-domain and observed 10 and one animals, respectively, in 232 Pakistani domestic goats. These remarkable deletion/insertion events would be useful to investigate mtDNA diversity within the highly divergent mt-lineage A. In analysis of the control region feature, the caprine mtDNA was A/T rich as observed for other artiodactyls. Four conserved regions, Block A in the L domain, the C-domain, Block B and CSB1 in the R domain, were defined as well as in the other species.Aug. 2004, Animal Science Journal, 75(4) (4), 303 - 309, EnglishScientific journal
- BLACKWELL PUBLISHING LTD, Aug. 2004, ANIMAL GENETICS, 35(4) (4), 356 - 358, EnglishScientific journal
- In order to clarify the origin and genetic diversity of cattle in North Eastern Asia, this study examined mitochondrial displacement loop sequence variation and frequencies of Bos taurus and Bos indicus Y chromosome haplotypes in Japanese, Mongolian, and Korean native cattle. In mitochondrial analyses, 20% of Mongolian cattle carried B. indicus mitochondrial haplotypes, but Japanese and Korean cattle carried only R taurus haplotypes. In contrast, all samples revealed R taurus Y chromosome haplotypes. This may be due to the import of zebu and other cattle during the Mongol Empire era with subsequent crossing with native taurine cattle. B. taurus mtDNA sequences fall into several geographically distributed haplogroups and one of these, termed here T4, is described in each of the test samples, but has not been observed in Near Eastern, European or African cattle. This may have been locally domesticated from an East Eurasian strain of Bos primigenius. (C) 2004 Elsevier Inc. All rights reserved.ACADEMIC PRESS INC ELSEVIER SCIENCE, Aug. 2004, MOLECULAR PHYLOGENETICS AND EVOLUTION, 32(2) (2), 539 - 544, EnglishScientific journal
- In the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. This paper describes the development of DNA markers to discriminate between Japanese Black and F1 (Japanese Black x Holstein) breeds. Amplified fragment length polymorphism method was employed to detect candidate markers absent in Japanese Black but present in Holstein. The 500 primer combinations yielded six selected markers that were converted into single nucleotide polymorphisms markers for high-throughput genotyping. The allele frequencies in both breeds were investigated for discrimination ability using PCR-RFLP. The probability of identifying F1 was 0.882 and probability of misjudgment was 0.0198. The markers could be useful for discriminating between Japanese Black and F1 and would contribute to the elimination of falsified breed labeling of meat. (C) 2003 Elsevier Ltd. All rights reserved.ELSEVIER SCI LTD, Jun. 2004, MEAT SCIENCE, 67(2) (2), 275 - 280, EnglishScientific journal
- The effect of cattle breed on stearoyl-CoA desaturase (SCD) gene expression was investigated in this study. Detailed comparisons of SCD mRNA level were made among three steers each of Japanese Black, Holstein and their crossbreed which were age-matched had been fed the same diet and were sampled by biopsy of the longissimus dorsi (LD) muscle and subcutaneous fat. The levels of SCD mRNA were measured in samples of muscle and subcutaneous fat. The levels of SCD mRNA demonstrated a breed effect in each tissue, though the relative expression was higher (P < 0.05) in subcutaneous fat. The ratio of SCD mRNA to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA for Japanese Black, crossbreed and Holstein were 132.1 +/- 34.1, 73.5 +/- 22.7 and 39.5 +/- 12.9, respectively, and significant (P<0.05) differences existed between Japanese Black and Holstein cattle. Japanese Black subcutaneous fat had consistently higher (P<0.05) monounsaturated fatty acids (MUFA) percentage than Holstein subcutaneous fat. These results suggest that differences in SCD gene expression may contribute to the fatty acid compositional differences seen between subcutaneous adipose tissue of Japanese Black cattle and Holstein. (C) 2003 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, May 2004, LIVESTOCK PRODUCTION SCIENCE, 87(2-3) (2-3), 215 - 220, EnglishScientific journal
- Isolation and linkage mapping of coding sequences from chicken cosmids by exon trappingWe performed exon trapping in order to locate functional genes on chicken chromosomes (GGA) and to identify functional gene sequences from chicken cosmids. Sequence analysis of 100 clones revealed 17 putative exons, five of which were identified with known sequences in a gene database search: thymopoietin beta (TMPO), U5 snRNP-specific 40 kDa protein (HPRP8BP), dihydropyridine receptor alpha 1 subunit (CACNLIA3), cystein string protein (CPS) and C15orf4. We attempted to map the genes to chicken chromosomes by using FISH and linkage analysis. The chromosomal localizations were GGA1 (TMPO), GGA10 (C15orf4), GGA23 (HPRP8BP) and GGA28 (CPS) by FISH and linkage analysis, while that of CACNLIA3 was predicted to be on a microchromosome by FISH but not by linkage analysis. Comparative mapping analyses between chickens and humans for the genes revealed both known and new synteny. The syntenic conservation,between GGAI and human chromosome (HSA) 12q23 (TMPO) and between GGA10 and HSA 15q25 (C15orf4), were consistent with a recent publication, while two new syntenies were observed between GGA28 and HSA20q13.3 in CPS and between GGA23 and HSAI p34-35 in HPPP8BP. The information of presently mapped genes can contribute as anchor markers based on functional genes and the construction of a comparative map.ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOCIETIES, Mar. 2004, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 17(3) (3), 309 - 312, EnglishScientific journal
- To investigate the genetic factors that affect fatty acid composition of beef, we compared the full-length bovine stearoyl-CoA desaturase (SCD) cDNA from 20 Japanese Black steers. Two types of the SCD gene with single nucleotide polymorphisms (SNPs) were observed in the ORF of SCD cDNA, in which an amino acid replacement from valine (type V) to alanine (type A) was predicted. We developed a method for genotyping these two SCD genes based on PCR-RFLP. We have classified 1003 Japanese Black carcasses into three genotypes, VV, VA, and AA, and compared fatty acid composition among them. The SCD type A gene contributed to higher MUFA percentage and lower melting point in intramuscular fat. The SCD genotype was not the only genetic factor contributing to fatty acid composition of Japanese Black steers, but the SCD genotype was considered one of the causes of genetic variation in fatty acid composition of Japanese Black steers. Transcription factors such as sterol regulatory element binding protein-1c (SREBP-1c) may account for the remaining part of the genetic variation in fatty acid composition.SPRINGER-VERLAG, Feb. 2004, MAMMALIAN GENOME, 15(2) (2), 142 - 148, EnglishScientific journal
- Using amplified fragment length polymorphism (AFLP) fingerprinting, selective genotyping was performed to determine if this method was effective for selecting superior breeding stock. Forty-eight cows with extreme genetic merit for beef marbling score (BMS) were selected from a population of Japanese Black cattle (n = 4462), including 25 with the highest for predicted breeding value (PBV) and 23 with the lowest. Sixteen AFLP fragments were selected for further analysis based on fragment frequency differences between the high and low groups. A linear discriminant analysis using these AFLP fragments was applied in order to derive a discriminant function that classified the cows into high and low groups. Seven of the 16 fragments were included in the resulting function and the discriminant scores (general genetic values, GGV) of the 48 cows were calculated using the function. These cows were clearly separated into high and low groups by GGV with a correlation ratio of 0.91 (discriminative error of 2.1%). The same function was then applied to 121 additional cows that were randomly selected from the original population. A significant regression coefficient of GGV on BMS-PBV (R-2 = 0.45) was obtained, which indicates that the GGV can be used as a selection criterion for BMS in this population. These results suggest that AFLP fingerprinting can be used for animal breeding without identifying the underlying genes affecting the trait of interest.BLACKWELL PUBLISHING LTD, Feb. 2004, ANIMAL GENETICS, 35(1) (1), 40 - 43, EnglishScientific journal
- Japanese Society of Animal Breeding and Genetics, 2004, The journal of animal genetics, 31(2) (2), 33 - 39
- On the basis of sequence variation in the displacement loop region of mtDNA, 588 Japanese and North American Holstein cows were classified into 5 mitochondrial haplotypes, which were found in Japanese Black cattle. One of the haplotypes (named type 1), which was present at the highest frequency in Japanese Black cattle, was not observed in either European or African cattle. This haplotype is characterized by 2 single-nucleotide polymorphisms. One is called the type B polymorphism, and it refers to a base change from T to C at nucleotide 16042 of the mitochondrial genome (T160042C). The other is called the type I polymorphism, and it refers to the base change as G16093A. The proportion of the Japanese Holstein population with both polymorphisms was 18.3%, whereas none of the North American cows had this genotype. Because the mitochondrial types were inherited maternally, it is clear that a considerable number of Japanese Holstein cows are descended from native Japanese cattle. Polymorphisms B and I accounted for no variance in the estimated breeding value for milk production among cows from the Hyogo herd (582 cows) or the Chiba region herd (758 cows). This result suggests that most autosomal genes of native animals have been successively replaced by those of pure Holstein after grading up of over 15 generations, even though resulting animals have native animal-oriented mitochondrial types and may still have some number of the native autosomal genes.American Dairy Science Association, 2004, Journal of Dairy Science, 87(9) (9), 3071 - 3075, EnglishScientific journal
- 2004, Rep. Soc. Res. Native Livestock., 21, 129-133, EnglishStudy on wooden and bamboo bells for the cattle kept in Myanmar.Scientific journal
- 2004, Rep. Soc. Res. Native Livestock., 21, 313-325, EnglishStudy on the origin and phylogeny of domestic goats.Scientific journal
- 2004, Proc. 29th Int. Conf. on Anim. Genet., p.138, EnglishSelective genotyping for marker assisted selection in Japanese Black cattle.International conference proceedings
- 2004, Rep. Soc. Res. Native Livestock., 21, 155-169, EnglishMorphological traits and biochemical Polymorphisms of Myanmar sheep.Scientific journal
- 2004, Rep. Soc. Res. Native Livestock., 21, 195-204, EnglishMorphological study on the external characteristics of Myanmar's native pig population.Scientific journal
- 2004, Rep. Soc. Res. Native Livestock., 21, 205-211, EnglishMorphological and genetic studies using small samples on the Indian wild pig (Sus scrofa cristatus) in Myanmar.Scientific journal
- 2004, Rep. Soc. Res. Native Livestock., in press, EnglishMitochondrial DNA Diversity of Native Goat in Myanmar.Scientific journal
- 2004, Animal Genetics., in press, 121 - 127, EnglishLinkage mapping of four chicken calpain genes.Scientific journal
- 2004, Rep. Soc. Res. Native Livestock., 21, 171-194, EnglishGene constitution of the native goats of Myanmar.Scientific journal
- 2004, Rep. Soc. Res. Native Livestock., 21, 135-154, EnglishGene constitution of the horse in Myanmar.Scientific journal
- 2004, Rep. Soc. Res. Native Livestock., 21, 225-236, EnglishGene constitution of egg white proteins of native chicken population in Myanmar.Scientific journal
- 2004, Rep. Soc. Res. Native Livestock., 21, 123-127, EnglishCytogenetic analysis of mithan on Myanmar.Scientific journal
- 2004, Rep. Soc. Res. Native Livestock., 21, 107-121, EnglishComposition of the gene controlling blood protein types of native water buffaloes in Myanmar and their phylogenetic study.Scientific journal
- 2004, Rep. Soc. Res. Native Livestock., 21, 213-223, EnglishComplete sequences of mitochondrial D-loop region of red junglefowls(Gallus gallus) and their genetic diversity in Myanmar and its neighbor countries.Scientific journal
- 2004, Proc. 29th Int. Conf. on Anim. Genet., p.70, EnglishComparisons between Japanese Black and Holstein cattle using gene expression profiling.International conference proceedings
- 2004, Rep. Soc. Res. Native Livestock., 21, 77-89, EnglishComparison of gene controlling blood protein types of native cattle and Mithan in Myanmar and their phylogenetic study.Scientific journal
- 2004, Rep. Soc. Res. Native Livestock., 21, 59-75, EnglishCoat-Color variation and body measurement of the native cattle in Myanmar.Scientific journal
- 2004, Rep. Soc. Res. Native Livestock., 21, 101-105, EnglishCoat-color polymorphism in water buffaloes in Myanmar.Scientific journal
- 2004, Rep. Soc. Res. Native Livestock., 21, 245-256, EnglishCoat-color and other morphogenetic polumorphisms in the cats of Myanmar.Scientific journal
- 2004, Rep. Soc. Res. Native Livestock., 21, 237-243, EnglishBlood protein variations of native fowls in Myanmar.Scientific journal
- 2004, Rep. Soc. Res. Native Livestock., 21, 91-99, EnglishAn assay for paternal gene flow between the taurus and indicus cattle in Myanmar using polymorphisms in SRY gene.Scientific journal
- Mitochondrial DNA diversity of Pakistani goatsPakistan contains numerous domestic goat breeds, but until now there has been no comprehensive study on genetic diversity or a phylogenetic analysis of Pakistani goats. In this study, we analysed the complete mitochondrial DNA D-loop and the cytochrome b gene of 13 Pakistani domestic goat breeds (Capra hircus) and one wild goat, the Sindh Ibex (Capra aegagrus blythi). The phylogenetic analyses and sequence divergence (SD) established four distinct mt-lineages termed as A, B and C (previously reported) and a new lineage D. The Sindh Ibex appeared as an outgroup of domestic goats. The estimated divergence times between the most recently evolved mt-lineages A and D were from 260 483 to 371 052 YA. This suggested that at least four different strains of wild Capra might have been the source of the modern domestic goats. The new mt-lineage D revealed high SD from mt-lineage A and may be the oldest branch under domestication, while mt-lineages B and C showed lower SD and might have been domesticated during an advanced stage of the domestication process.BLACKWELL PUBLISHING LTD, Dec. 2003, ANIMAL GENETICS, 34(6) (6), 417 - 421, EnglishScientific journal
- Analyses of beta-1 syntrophin, syndecan 2 and Gem GTPase as candidates for chicken muscular dystrophyDespite intensive studies of muscular dystrophy of chicken, the responsible gene has not yet been identified. Our recent studies mapped the genetic locus for abnormal muscle (AM) of chicken with muscular dystrophy to chromosome 2q using the Kobe University (KU) resource family, and revealed the chromosome region where the AM gene is located has conserved synteny to human chromosome 8q11-24.3, where the beta-1 syntrophin (SNTB1), syndecan 2 (SDC2) and Gem GTPase (GEM) genes are located. It is reasonable to assume those genes might be candidates for the AM gene. In this study, we cloned and sequenced the chicken SNTB1, SDC2 and GEM genes, and identified sequence polymorphisms between parents of the resource family. The polymorphisms were genotyped to place these genes on the chicken linkage map. The AM gene of chromosome 2q was mapped 130 cM from the distal end, and closely linked to calbindin 1 (CALB1). SNTB1 and SDC2 genes were mapped 88.5 cM distal and 27.6 cM distal from the AM gene, while the GEM gene was mapped 18.5 cM distal from the AM gene and 9.1 cM proximal from SDC2. Orthologues of SNTB1, SDC2 and GEM were syntenic to human chromosome 8q. SNTB1, SDC2 and GEM did not correspond to the AM gene locus, suggesting it is unlikely they are related to chicken muscular dystrophy. However, this result also suggests that the genes located in the proximal region of the CALB1 gene on human chromosome 8q are possible candidates for this disease.INT PRESS EDITING CENTRE INC, Oct. 2003, EXPERIMENTAL ANIMALS, 52(5) (5), 391 - 396, EnglishScientific journal
- (公社)日本畜産学会, Mar. 2003, 日本畜産学会大会講演要旨集, 101回, 101 - 101, Japaneseミャンマーの在来家畜に関する研究(1) ミャンマーにおける在来家畜の飼養概況
- (公社)日本畜産学会, Mar. 2003, 日本畜産学会大会講演要旨集, 101回, 102 - 102, Japaneseミャンマーの在来家畜に関する研究(6) ミャンマー馬の遺伝子構成
- (公社)日本畜産学会, Mar. 2003, 日本畜産学会大会講演要旨集, 101回, 103 - 103, Japaneseマイクロサテライト分析によるミャンマー在来ヤギの遺伝的多様性
- (公社)日本畜産学会, Mar. 2003, 日本畜産学会大会講演要旨集, 101回, 198 - 198, Japaneseミタン(Bos gaurus)のプリオン遺伝子(PRNP)について
- 日本食肉研究会, 2003, 食肉の科学, 44:1-5(2) (2), 175 - 179, Japanese牛肉の品種判別技術についてScientific journal
- 日本動物遺伝育種学会, 2003, 動物遺伝育種研究, 30:17-20.(2) (2), 17 - 20, JapaneseScientific journal
- 2003, Rep. Soc. Res. Native Livestock., 未記入, EnglishMitochondrial DNA Diversity of Native Goat in Myanmar.Scientific journal
- Identification of mitochondrial DNA substitutions related to meat quality in Japanese Black cattleComplete sequences of mitochondrial (mt) genomes of eight Japanese Black cattle were determined to investigate the relationships between mt deoxyribonucleic acid (DNA) displacement loop (D-loop) types and other mtDNA regions and to identify the variation in the coding region that may influence the economic traits. The survey of mitochondrial sequences in the encoding region revealed 14 substitutions including six antonymous substitutions and one in 16S ribosomal ribonucleic acid (rRNA). Three methods of polymorphic DNA analyses (polymerase chain reaction [PCR]-restriction fragment length polymorphism [RFLP], mismatch PCR-RFLP, PCR-single-strand conformation polymorphism [SSCP]) were performed on these seven candidate substitutions (base pair [bp] 2,232, 12,158, 12,908, 13,310, 14,122, 14,140, and 14,565) for 202 Japanese Black cattle. The substitution of by 13,310 was observed in all samples, but not in the reference sequence, indicating that this is a minor substitution or a sequencing mistake in the reference sequence. The substitutions at by 14,122, 14,140, and 14,565 were observed in only a few samples, suggesting that these were also minor substitutions. The substitutions at by 2,232 (16S rRNA), 12,158, and 12,908 (reduced nicotinamide adenine dinucleotide-ubiquinone oxidoreductase chain-5) were closely related to mitochondrial D-loop types that have previously been related to differences in the carcass traits of Japanese Black cattle. Evaluation of the effects on six carcass traits with mixed model procedures suggests that the by 2,232 substitution affects longissimus muscle area and beef marbling score. The substitution at by 2,232 is a strong candidate for the mitochondrial effect on meat quality.AMER SOC ANIMAL SCIENCE, Jan. 2003, JOURNAL OF ANIMAL SCIENCE, 81(1) (1), 68 - 73, EnglishScientific journal
- 2003, Mammalian Genome., 未記入, 142 - 148, EnglishGenotypes of stearoyl-CoA desaturase affect on the difference of fatty acid composition of Japanese Black steers.Scientific journal
- 農畜産業振興事業団企画情報部, 2003, 畜産の情報, 8:27-29(166) (166), 27 - 29, JapaneseDNA マーカーによる黒毛和種とF1 との品種識別Scientific journal
- 2003, Livest. Prod. Sci., 未記入, EnglishDifferences in Stearoyl-CoA Desaturase mRNA levels between Japanese Black and Holstein cattle.Scientific journal
- 2003, MeatScience., 未記入, EnglishDevelopment of Breed Identification Markers derived from AFLP in beef cattle.Scientific journal
- BLACKWELL PUBLISHING LTD, Aug. 2002, ANIMAL GENETICS, 33(4) (4), 312 - 312, EnglishLinkage mapping of the mitochondrial aconi-tase (ACO2) gene to chicken chromosome 1
- A chicken linkage map, constructed with the Kobe University (KU) resource family was used to locate the genetic locus for muscular dystrophy of abnormal muscle type (AM). The KU resource family is a backcross pedigree with 55 offspring produced from the mating of a White Leghorn F-line (WL-F) male and a hybrid female produced from a cross between the WL-F male and a female of the Fayoumi OPN line who was homozygous for the AM gene. In total, 872 loci were genotyped on the pedigree: 749 (86%) were informative and mapped to 38 linkage groups. These informative loci included 649 AFLPs, 93 MS, three functional genes, the AM locus, sex phenotype, and two red blood cell loci. The remaining 123 markers were unlinked. Nineteen of the 38 KU linkage groups were assigned to macrochromosomes 1-8 and 11 microchromosomes including chromosome W, while 19 linkage groups were unassigned. The total map was 3569 cm in length, with an average marker interval of 4.8 cm. The AM locus was mapped 130 cm from the distal end of chromosome 2q.2002, Animal Genetics, 33(1) (1), 42 - 48, EnglishScientific journal
- This article describes the complete sequences of the mitochondrial DNA displacement loop (D-loop) region and cytochrome b gene from domestic goats in Laos (Laos native) and wild goat "markhor" (C. falconeri). The wild goat "bezoar" (Capra aegagrus) has been considered to be the strongest candidate for the ancestor of the domestic goats (C. hircus) however, there is not sufficient molecular data to verify the hypothesis at present. In phylogenetic analyses, two wild goats, the markhor and the ibex (C. ibex), appeared as an outgroup, while the bezoar was located in a cluster of domestic goats. Mitochondrial haplotypes of Laos natives revealed two distinct major clusters: one was the same as the bezoar, the second, unique to Laos natives. The topology and calibrated levels of sequence divergence suggests that these clusters might represent at least two different subspecies of ancestral bezoars.2001, Biochemical Genetics, 39(5-6) (5-6), 145 - 154, English[Refereed]Scientific journal
- 2001, Anim. Sci. J., 72, 172Phylogenic analysis of five beef cattle breeds using AFLP marker.[Refereed]
- Japanese Society of Animal Breeding and Genetics, Nov. 2000, The journal of animal genetics, 28(1) (1), 95 - 100, Japanese
- Pig testicular lactate dehydrogenase-C (LDHC) cDNA was cloned and sequenced. The deduced sequence of 332 amino acids from pig LDHC shows 73% and 67% identity with that of pig LDHA (muscle) and LDHB (heart) respectively, whereas pig LDHA and LDHB isozymes shows 74% sequence identity. Pig and mouse LDHC cDNAs were subcloned into bacterial expression vector, and the expressed pig LDHC isozyme was shown to be as thermally stable as mouse LDHC isozyme. Pig genomic DNAs from Chinese Meishan, English Yorkshire, Danish Landrace and American Duroc were shown to exhibit polymorphic sites for restriction enzymes EcoRI, BamHI and PstI. (C) 2000 Elsevier Science B.V. All rights reserved.ELSEVIER SCIENCE BV, Jan. 2000, GENE, 242(1-2) (1-2), 151 - 154, EnglishScientific journal
- Mitochondrial DNA variation and evolution of Japanese black cattle (Bos taurus)This article describes complete mitochondrial DNA displacement loop sequences from 32 Japanese Black cattle and the analysis of these data in conjunction with previously published sequences from African, European, and Indian subjects. The origins of North East Asian domesticated cattle are unclear. The earliest domestic cattle in the region were Bos taurus and may have been domesticated from local wild cattle (aurochsen; B. primigenius), or perhaps had an origin in migrants from the early domestic center of the Near East. In phylogenetic analyses, taurine sequences form a dense tree with a center consisting of intermingled European and Japanese sequences with one group of Japanese and another of all African sequences, each forming distinct clusters at extremes of the phylogeny. This topology and calibrated levels of sequence divergence suggest that the clusters may represent three different strains of ancestral aurochs, adopted at geographically and temporally separate stages of the domestication process. Unlike Africa, half of Japanese cattle sequences are topologically intermingled with the European variants. This suggests an interchange of variants that may be ancient, perhaps a legacy of the first introduction of domesticates to East Asia.GENETICS, Nov. 1998, GENETICS, 150(3) (3), 1169 - 1175, English[Refereed]Scientific journal
- Chicken ornithine transcarbamylase gene, structure, regulation, and chromosomal assignment: Repetitive sequence motif in intron 3 regulates this enzyme activityOrnithine transcarbamylase (OTC) is one of the urea cycle enzymes. While the chicken is a uricotelic animal, it has measurable OTC activity in its kidney. OTC activity is highly variable within and between chicken breeds, Chicken OTC may have some physiological significance because of its significant activity in the kidney, We cloned the OTC cDNA from chicken kidney and found 77% homology between the deduced amino acid sequence of the mature protein and that of mammals. The chicken OTC gene spans 26 kb, consists of 10 exons and 9 introns, and utilizes the same exon-intron boundaries as the human gene, The 5'-fIanking region contains a putative TATA box and two potential regulatory sites, but neither the 5'-flanking region nor the splice sites correlated with variation in OTC activity. In intron 3, two polymorphic sites were found: one comprising a deletion of 401 nucleotides; and the other was a length and sequence polymorphic region located 8 bases upstream from the deletion. The latter polymorphism provides an explanation for phenotypic variation in OTC. Linkage analysis has suggested reassignment of the chicken OTC gene from the suggested Z chromosome to chromosome 1q.JAPANESE BIOCHEMICAL SOC, Nov. 1998, JOURNAL OF BIOCHEMISTRY, 124(5) (5), 962 - 971, EnglishScientific journal
- Japanese Society of Animal Breeding and Genetics, 1998, The Journal of Animal Genetics, 26(1) (1), 13 - 18, Japanese
- Japanese Black fattening steers were used to examine relationships between carcass traits and mitochondria displacement loop (D-loop) variations. The D-loop region of Japanese Black cattle was sequenced and revealed 26 mitochondrial haplotypes defined by 25 polymorphic sites. The haplotypes were classified into five mitochondrial types (type 1 to 5) using the unweighted pair-group method with arithmetic means. Carcass weight, longissimus muscle area (LMA), rib thickness, subcutaneous fat thickness, yield estimate, and beef marbling score (BMS) were compared among five mitochondrial types with BLUP procedures. Significant differences between mitochondria types were detected for LMA and BMS. Difference (P <.05) was observed between mitochondrial types 2 and 4 for LMA. There was a highly significant difference (P <.01) in EMS between types 2 and 4. Difference (P <.05) was also found between types 1 and 4 on BMS. These results suggest that cytoplasmic genetic effects are important sources of variation for carcass traits in Japanese Black cattle.AMER SOC ANIMAL SCIENCE, Jan. 1998, JOURNAL OF ANIMAL SCIENCE, 76(1) (1), 36 - 41, English[Refereed]Scientific journal
- BLACKWELL SCIENCE LTD, Dec. 1997, ANIMAL GENETICS, 28(6) (6), 456 - 456, EnglishA highly polymorphic minisatellite locus (KBMS2) in bovine[Refereed]Scientific journal
- The cDNA cloning and molecular evolution of reptile and pigeon lactate dehydrogenase isozymesThe cDNAs encoding lactate dehydrogenase isozymes LDH-A (muscle) and LDH-B (heart) from alligator and turtle and LDH-A, LDH-B, and LDH-C (testis) from pigeon were cloned and sequenced. The evolutionary relationships among vertebrate LDH isozymes were analyzed. Contrary to the traditional belief that the turtle lineage branched off before the divergence between the lizard/alligator and bird lineages, the turtle lineage was found to be clustered with either the alligator lineage or the alligator-bird clade, while the lizard lineage was found to have branched off before the divergence between the alligator/turtle and bird lineages. The pigeon testicular LDH-C isozyme was evidently duplicated from LDH-B (heart), so it is not orthologous to the mammalian testicular LDH-C isozymes.OXFORD UNIV PRESS, Nov. 1997, MOLECULAR BIOLOGY AND EVOLUTION, 14(11) (11), 1081 - 1087, English[Refereed]Scientific journal
- Japanese Society of Animal Breeding and Genetics, 1997, The Journal of Animal Genetics, 25(1) (1), 21 - 26, Japanese
- Japanese Society of Animal Breeding and Genetics, 1997, 動物遺伝研究会誌, 25, 16 - 23, Japanese
- Polymerase Chain Reaction (PCR) was applied to species identification of meats and meat products. For the identification of meat species by PCR, three sets of primer, i. e., BF (5'-GGATCCCTCTTCTCGCTCCG-3') and BR (5'-GGTTAAGCTACATCAACGGG-3') for cattle, PF (BF) and PR (5'-ACTGAATAGCACCTTGTTTGG-3') for pig, and also CF(5'-CCCCTTTCCCCAGGGGGGGT-3') and CR (5'-AGGGGTTGCTGATCTCTCGT-3') for chicken, were designed according to the published mitochondrial D-loop DNA sequences from cattle, pig and chicken. Amplification was carried out 35 cycles of denaturation at 94°C for 2min, annealing at 66-68°C for 2min, and extension at 72°C for 3min in the presence of Taq DNA polymerise. Products amplified were analyzed by 2% agarose gel electrophoresis. Products amplified from beef, pork and chicken gave single specific DNA fragment of 518bp, 321bp and 400bp, respectively. Using this method, several meat products were analyzed. Mixing of beef, pork or chicken in meat products was revealed by identification of each single specific DNA fragment. When three sets of primers were employed together, beef, pork and/or chicken specific DNA fragment from these mixed meat products could be identified by only one PCR reaction. Sheep is closely related species of cattle, but lamb does not gave any amplyfied DNA fragment under these experimental conditions. The detection limits in a mixed DNA sample were approximately 0.1% beef in pork, 0.001% pork in beef, 1% chicken in pork, respectively.Japanese Society of Animal Science, Oct. 1996, Nihon Chikusan Gakkaiho, 67(10) (10), 900 - 905, Japanese
- SMXA recombinant inbred (RI) strains of mice were produced by systematic inbreeding from the F2 generation of a cross between two progenitor inbred strains, A/J and SM/J, which differ considerably with respect to many characteristics, and consists of 28 inbred strains. In this study, we investigated the applicability of DNA fingerprinting with M13 phage DNA to the identification of these closely related strains. DNA fingerprints of the SMXA RI strains and their progenitors, SM/J and A/J, showed strain-specific patterns, with the same banding patterns within each strain. Linkage analysis by using strain distribution patterns of minisatellite loci with 108 genetic markers containing microsatellites, biochemical and immunological marker genes allowed 23 minisatellite loci to be assigned to 11 chromosomes. The results suggested that DNA fingerprinting with M13 phage DNA is applicable not only for strain identification but also for genetic monitoring of RI strains on almost all chromosomes.Japanese Association for Laboratory Animal Science, Apr. 1995, Proceedings of The Japanese Society of Animal Models for Human Diseases, 44(2) (2), 87 - 93, English
- Identification of substrains or sublines of inbred mice and assessment of genetic relationships among them were performed on the basis of DNA fingerprinting using M13 phage DNA as a probe. We used eight C57BL/6 sublines (J//Jcl, J//Jms, J//Sic, J//Nrs, Yok, Jah, N//Crj, N//Jcl) and eleven C3H/He sublines (J, J//Jcl, J//Yok, J//Nrs, Jms, N, N//Jcl, N//Crj, Sic, Jah, Nrs) . Two kinds of restriction endonucleases (HinfI andPstI) were used. It was found that : 1) DNA fingerprint within each subline showed identical patterns. 2) Most sublines of C57BL/6 and C3H/He could be identified using DNA fingerprinting withHinfI except between N//Crj and Sic, and among J, J//Nrs and J//Yok in C3H/He. DNA fingerprints withPstI endonucleases showed low polymorphic banding patterns. 3) A dendrogram constructed from DNA fingerprint patterns reflected generally the genealogy of the sublines used. 4) DNA fingerprinting, therefore, seemed to be suitable for the genetic monitoring and assessment of genetic relationships among sublines of inbred mice having close relationships.Japanese Association for Laboratory Animal Science, 1994, Experimental Animals, 43(4) (4), 521 - 526
- GENETIC SIMILARITY USING DNA FINGERPRINTING IN CATTLE TO DETERMINE RELATIONSHIP COEFFICIENTThe relation between relationship coefficient and genetic similarity was evaluated using DNA fingerprinting in Japanese Black cattle. We proposed a new method for evaluating heterozygous and homozygous minisatellite loci. Genetic similarity by DNA fingerprinting was estimated by two methods-band sharing (BS) and a new evaluation called genetic similarity (GS). Linear regressions of the relationship coefficient (y) on levels of BS (x1) and GS (x2) yielded the equations y = 0.049 + 0.684x1 and y = -0.206 + 1.149x2, with highly significant correlation coefficients of .631 and .813, respectively. It was suggested that DNA fingerprinting could be applied to estimate the relationship coefficient not only in cattle but also in other species.OXFORD UNIV PRESS INC, May 1993, JOURNAL OF HEREDITY, 84(3) (3), 166 - 169, English[Refereed]Scientific journal
- Using two lines of chicken, 52 line originated from White Cornish and WL-B line originated from White Leghorn, we examined DNA fingerprints probed with five chemically synthesized repetitive sequences (M13, YNZ22, mo-1, 33.15 and alpha-globin) comparing with that probed with M13 phage repetitive sequence. To make each probe, an oligonucleotide of each repetitive unit less than 20 bases was syn-thesized using a DNA synthesizer with a complementary oligonucleotide, and after phosphorylation they were annealed and ligated. The ligated DNA fragment was amplyfied by PCR using the oligonucleotides as primers. The amplyfied DNA frag-ments are from 0.5 to 20kbp. DNA fingerprints probed with three repetitive sequences of YNZ22, mo-1 and alphaglobin showed clear and well separated numerous bands as well as that probed with M13 phage repetitive sequence. Since the number of bands clearly detectable are increased up to 393 as a total, it is possible to make a linkage analysis between these markers and some qualitative or quantitative traits. DNA fingerprints probed with the repetitive sequence revealed a characteristic pattern in each chicken and in line, indicating that the method described here was useful for parentage analysis and for elucidaing of the genetic structure of a chicken strain.Japan Poultry Science Association, 1993, The Journal of Poultry Science, 30(4) (4), 287 - 297, Japanese
- We carried out parentage testing with DNA fingerprinting in two breeds of horse (Throughbred and Percheron) using three probes (M13 repetitive sequence, core sequence and MSl) and four restriction endonucleases (Hae III, Hinf I, Msp I and Pst I) . Using the core probe, extremely low polymorphisms were found in all restriction endonucleases. With the M13 probe, a few available fragments for parentage testing were detected in DNA fingerprints (DFPs) with Hae III and Msp I endonucleases. Using the MS1 probe, distinct DFPs fragments were found, but their polymorphism was found to be low. The results suggest that polymorphism in horses is lower than in the other species, such as chicken, cattle and mouse.Japanese Society of Animal Breeding and Genetics, 1993, The Journal of Animal Genetics, 21(1) (1), 39 - 43, English
- Using bovine cDNA of lipoprotein lypase (LPL) (triacyl glycero protein acylhydrolase, EC 3.1.1.34) as a probe, restriction fragment length polymorphisms (RFLP) have been examined on a herd of Japanese Black cattle. The LPL cDNA insert of plasmid pCD-LPL was divided into two halves using restriction enzymes Bam H1 and Sma I; one is 1.3kb 5'-half containing open reading frame and the other is 1.9kb 3'-half containing 3' non-translated region. Using the 1.9kb fragment as a probe a RFLP was observed only with Hind III out of 9 restriction enzymes examined. The 1.3kb probe gave distinctive RFLPs with Pst I, Hae III, Hind III, Taq I and Msp I. According to RFLP, animals could be divided into three genotypes AA, AB and BB with any of restriction enzymes shown above. Types of RFLP being given with each restriction enzyme related to each other and were expressed as clusters A and B, suggesting these RFLP types observed here are linked each other. It is quite possible that a transposon like insert having five different restriction sites shown above is invertedly inserted at a given site of the LPL gene. Heifers of 116 Japanese Black of a breeding herd were classified into three groups, 15 of AA, 65 of AB and 35 of BB. Gene frequencies of clusters A and B were estimated as 0.418 and 0.582, respectively. It was suggested by an analysis of twenty families from the same herd that A and B RFLP types are codominantly inherited. No relationships between RFLP types and beef marbling standard (BMS) were observed using 20 steer carcasses of Japanese BlackJapanese Society of Animal Science, 1993, Nihon Chikusan Gakkaiho, 64(5) (5), 474 - 479, Japanese
- We have cloned cDNAs of porcine lactate dehydrogenase isozymes A and B; their sizes are 1684bp and 1320bp, respectively. These cDNAs were subcloned into Bluescript SK- vector and amplified by PCR using T3 and T7 primers. Using the amplified cDNAs as probes, we examined restriction fragment length polymorphisms (RFLPs) at the porcine LDH genes. With LDH-A and -B probes, RFLPs were observed with the following five restriction enzymes; Bam HI, Msp I, Pst I, Hae III, and Eco RI. To test five restriction enzymes at the same time, only one pig was sampled from each of the Meishan, Jinhua, and Landrace breeds. However, their RFLPs seemed to be different each other. Using Msp I, RFLPs in a crossbred family of Jinhua and Meishan composed of parents and six piglets were also examined for parentage test. LDH-A probe represents 13 fragments, whereas LDH-B probe nine fragments. Out of these 22 fragments, thirteen fragments were commonly shared between parents and nine fragments were originated from either of the parents, indicating that the latter fragments are available for parentage test. We also examined DNA fingerprints on the same crossbred family to evaluate usefulness of RFLPs of LDH isozyme genes. Using M13 repeated sequences as a probe, 17 to 18 fragments were observed in the DNA fingerprints when with Msp I. Seven out of these fragments were available for the parentage test. From these results, it was concluded that porcine LDH cDNAs are useful probes not only for parentage test but also for linkage analysis.Japanese Society of Animal Science, 1993, Nihon Chikusan Gakkaiho, 64(11) (11), 1109 - 1114, Japanese
- It is well known that laboratory rabbits are not controlled genetically like laboratory mice and rats. In order to test the usefulness of DNA fingerprinting in investigation of genetic uniformity of the laboratory rabbits strains and their relationships, we applied DNA fingerprinting using bacteriophage M13 probe to five strains (2 inbreds (JWY-NIBS and DuY-NIBS) and 3 outbreds (JW-NIBS, Icl: JW and WHHL) ) . DNA fingerprints of 2 inbred strains showed the same banding patterns within each strain but the strain-specific patterns. Although there were no rabbits showing the same banding patterns in 3 outbred strains, average percent differences (APD) were 13.7 to 18.6. A dendrogram based on APD of DNA fingerprints was constructed by 2 large clusters, JW group and DuY. The dendrogram was essentially similar to that based on rabbit mandible measurements. These results suggest that DNA fingerprinting is available not only for the genetic monitoring of the laboratory rabbit strains but also for the investigation of their genetic relationships.Japanese Association for Laboratory Animal Science, 1993, Experimental Animals, 42(3) (3), 337 - 342
- Features of a long-haired mutant and inheritance of mutation were investigated in the golden hamster (Mesocricetus auratus) . The hair of adult long-haired hamsters was longest at the rump and flanks (approximately 70 mm) and was also unusually long at the neck (45 to 50 mm) . The hair length of males was more striking than that of females. Mating experiments indicated that an autosomal recessive gene is responsible for the inheritance of long hair. An albino long-haired hamster strain was established by continuous full-sib mating from F2 hybrids, descendants of (acromelanic albino x agouti long-haired) F1 progeny, and genetic homogeneity was confirmed by DNA fingerprinting. The long-haired strain was characterised by the fact that males grew larger than females, in contrast to the other strains of hamsters.Japanese Association for Laboratory Animal Science, 1993, Experimental Animals, 42(3) (3), 343 - 347
- Strain identification and the assessment of genetic relationships among inbred strains of mice were examined by DNA fingerprinting using M13 phage DNA as a probe. We used 8 inbred strains (NC/Jah, DDD/Jah, DSD/Jah, RR/Jah, SS/Jah, C3H/HeJah, C57BL/6 Jah and C57BL/6CrSlc) and coisogenic (NC-brp) as well as congenic (C57BL/6-bg) strains. DNA fingerprints using M13 phage DNA as a probe revealed identical patterns within strains. Bands due to new mutations in minisatellite regions within each strain were not observed. The banding patterns were strain-specific. Thus, DNA fingerprinting seemed to be suitable for genetic monitoring of mice. A dendrogram constructed with difference values calculated from DNA fingerprint patterns showed that relationships among strains reflected the history of these strains, so DNA fingerprinting could also be effectively used to assess the relationships between inbred strains of mice.Japanese Association for Laboratory Animal Science, 1993, Experimental Animals, 42(2) (2), 169 - 173
- SMXA recombinant inbred (RI) strains were derived from the F2 generation of a cross between two progenitor inbred strains, A/J and SM/J, which differ considerably with respect to many characters. In order to determine the morphometric profiles of the mandible of SMXA RI strains and to identify these strains, principal component and discriminant analyses (mandible analysis) were carried out using 11 mandible measurements. Principal component analysis revealed that the mandibles of each strain have their own characteristic size and shape. The RI strains, however, were roughly divided into 15 groups based on the morphometric profile of their mandibles. Discriminant analysis showed the probability of erroneous discrimination to be 6.49% (20/308) for the males and 8.06% (27/335) for the females. In both sexes, most of the erroneous discrimination (13 in the male, 17 in the female) occurred between RI strains having comparatively similar morphometric profiles. This suggested that all of the SMXA RI strains examined could be regarded as genetically homogeneous within each strain.Japanese Association for Laboratory Animal Science, 1993, Experimental Animals, 42(1) (1), 41 - 50
- Genomic DNA was extracted from laboratory animals (rabbit, mastomys and Syrian hamster) and digested with several restriction endonucleases. DNA fingerprinting was applied to the digested DNA using repetitive sequence in the bacteriophage M13 as a probe. As a result, the banding patterns in these DNA fingerprints were highly polymorphic and specific to each individual. The same banding pattern was observed in the liver DNA and the blood DNA in the same individual. From these results, this method seems to be usefull in strain identification and genetic monitoring not only for laboratory mouse and rat but also for developing laboratory animals such as rabbit, mastomys and hamsters. Parentage test of two rabbit families (Japanese white × New Zealand white) confirmed that all bands of Fl were derived from either parent.Japanese Society of Animal Breeding and Genetics, 1992, The Journal of animal genetics, 1992(20) (20), 27 - 31
- A DNA fingerprint analysis of cattle using the repetitive sequence of M 13 phage DNA as a probe revealed 15 to 22 bands, ranging between 1 and 20 kilobases in size, in each individual examined. DNA fingerprints of three cattle breeds, Japanese Black, Japanese Brown and Holstein, showed high variability and specific banding pattern for each individual. The probabilities of shared band (Sb) for DNA fingerprints to estimate genetic similarity between individuals were 0.659±0.093 for Japanese Black, 0.714±0.056 for Japanese Brown, 0.643±0.155 for Holstein, and the Sb between breeds were 0.580±0.068 for Japanese Black and Japanese Brown, 0.520±0.056 for Japanese Black and Holstein, and 0.535±0.079 for Japanese Brown and Holstein. DNA fingerprint analysis of four Japanese Black families showed that all bands of offspring originated from those of either parent. The number of common bands between individuals increased with increasing coefficient of relationship between them. These results indicate that DNA fingerprinting using M 13 phage repetitive sequence is a powerful tool for identifying individuals and parentage test in cattle.Japanese Society of Animal Science, 1992, Nihon Chikusan Gakkaiho, 63(9) (9), 928 - 936, Japanese
- Japanese Society of Animal Breeding and Genetics, 1991, The Journal of animal genetics, 1991(19) (19), 11 - 18
- Genomic DNA was extracted from three breeds of chickens and digested with restriction enzyme, Hae III. DNA fingerprinting was applied to the digested DNA using 15 base pair repeats in the bacteriophage M13 as a probe. These repeats detected 25 to 35 bands, between 0.5kbp and 10kbp, in each chicken examined. Although banding pattern in the DNA fingerprint is specific to each individual, several common bands were found within the same breed. A parentage test was carried out with parent-offspring DNA fingerprints. All bands of F1 embryo were always originated from either parent. The similar banding pattern in DNA fingerprint was observed between the germ cell and the differentiated cell in the same individual. From the above results, the method described here seemed to be useful for identifying the individual and parent-offspring.Japanese Society of Animal Science, 1990, Nihon Chikusan Gakkaiho, 61(12) (12), 1057 - 1062, Japanese
- Lead, May 2019, 畜産技術, 768, 12 - 15, JapanesemtDNAハプログループPの存在から推測される日本北部在来牛の起源2019[Invited]Introduction scientific journal
- 2019, 日本畜産学会大会講演要旨, 125th複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討
- Oct. 2018, 畜産技術, 761(10) (10), 11 - 14, JapaneseオーストラリアWAGYU 牛肉に対するDNA 鑑定法の適用[Refereed]Introduction scientific journal
- In Japan, Wagyu cattle include four Japanese breeds; Black, Brown, Shorthorn, and Polled. Today, the renowned brand name Wagyu includes not only cattle produced in Japan, but also cattle produced in countries such as Australia and the United States. In recent years, the intramuscular fat percentage in beef (longissimus muscle) from Japanese Black cattle has increased to be greater than 30%. The Japanese Black breed is genetically predisposed to producing carcass lipids containing higher concentrations of monounsaturated fatty acids than other breeds. However, there are numerous problems with the management of this breed including high production costs, disposal of untreated excrement, the requirement for imported feed, and food security risks resulting from various viral diseases introduced by imported feed. The feeding system needs to shift to one that is more efficient, and improves management for farmers, food security for consumers, and the health environment for residents of Japan. Currently, we are developing a metabolic programming and an information and communications technology (ICT, or Interne of Things) management system for Wagyu beef production as future systems. If successful, we will produce safe, high-quality Wagyu beef using domestic pasture resources while solving the problems of how to utilize increasing areas of abandoned agricultural land and to make use of the plant-based feed resources in Japan's mountainous areas.Last, Jul. 2018, Asian-Australasian journal of animal sciences, 31(7) (7), 933 - 950, English, International magazine[Refereed]
- 2018, 日本畜産学会大会講演要旨, 124thmtDNA塩基配列を用いたカザフスタン在来ウシの系統遺伝学的解析
- 2018, 日本畜産学会大会講演要旨, 124th兵庫県黒毛和種集団におけるSLC27A6遺伝子多型のロース脂肪割合に対する効果の検討
- 2018, 日本畜産学会大会講演要旨, 124thウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索
- 農林水産・食品産業技術振興協会, Sep. 2017, JATAFFジャーナル = JATAFF journal : 農林水産技術, 5(9) (9), 31 - 34, JapaneseGenome analysis and utilization of the DNA markers in Wagyu
- Sep. 2017, JATAFFジャーナル, 5(9) (9), 31 - 34, Japanese和牛のゲノム解析[Refereed]Introduction scientific journal
- Sep. 2017, J Anim Breed Genomics, 1(1) (1), 17 - 22, EnglishThe Genetic Diversity of Japanese Wagyu using Molecular Markers[Refereed]Introduction scientific journal
- 畜産技術協会, Apr. 2017, 畜産技術, 743(743) (743), 2 - 6, Japanese高密度SNPアレイを用いた口之島野生化牛の遺伝的多様性評価[Refereed]Introduction scientific journal
- 2017, 日本畜産学会大会講演要旨, 122ndカザフスタンにおける在来馬調査の概要
- 2017, 日本畜産学会大会講演要旨, 122ndmtDNA配列を用いたカザフスタン5地域の在来ヤギにおける遺伝的構造の比較
- 2017, 日本畜産学会大会講演要旨, 122ndカザフスタン在来家畜とその近縁野生種の遺伝資源学的国際協同調査研究の概要
- 2017, 日本畜産学会大会講演要旨, 122ndカザフスタン在来ヤギのマイクロサテライトDNA多型
- 2017, 日本畜産学会大会講演要旨, 122ndカザフスタン在来牛のマイクロサテライトDNA多型
- AMER SOC ANIMAL SCIENCE, Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 21 - 21, EnglishIdentification of SNP associated with fertility trait using pool-based genome-wide association study in Japanese Black cattle.Summary international conference
- AMER SOC ANIMAL SCIENCE, Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 17 - 18, EnglishSummary international conference
- AMER SOC ANIMAL SCIENCE, Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 18 - 18, EnglishSummary international conference
- AMER SOC ANIMAL SCIENCE, Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 17 - 17, EnglishSummary international conference
- Oct. 2014, Animal Frontiers, 4(4) (4), 46 - 54, EnglishMeat Produced by Japanese Black Cattle and Wagyu[Refereed]Introduction scientific journal
- 岡山実験動物研究会, Apr. 2014, 岡山実験動物研究会報, (30) (30), 68 - 69, Japaneseニワトリ筋ジストロフィーに関する研究
- 日本動物遺伝育種学会, 01 Dec. 2012, 動物遺伝育種研究, 40(2) (2), 78 - 78, JapaneseウシFABP4遺伝子のプロモーター領域に対する多型探索
- 01 Dec. 2012, 動物遺伝育種研究, 40(2) (2), 73, Japanese岐阜県黒毛和種集団におけるUTS2R遺伝子内多型の脂肪酸組成に対する効果
- 01 Dec. 2012, 動物遺伝育種研究, 40(2) (2), 85, Japanese黒毛和種におけるLEP遺伝子内多型の脂肪酸組成に対する効果
- 01 Dec. 2012, 動物遺伝育種研究, 40(2) (2), 72, Japanese黒毛和種におけるFASN遺伝子内多型の脂肪酸組成に対する効果
- 01 Dec. 2012, 動物遺伝育種研究, 40(2) (2), 71, Japanese黒毛和種の枝肉形質に対するSCD,SREBP1,FASNおよびNCAPG遺伝子多型の関連解析
- 30 Jun. 2012, 食肉の科学, 53(1) (1), 125 - 126, Japaneseおいしい牛肉や牛肉偽装を見抜くDNA診断法の開発
- (公社)日本畜産学会, Mar. 2012, 日本畜産学会大会講演要旨集, 115回, 118 - 118, Japaneseプーリング法を用いた高密度SNP解析による黒毛和種閉鎖集団の遺伝的多様性の評価
- Genes Associated with Fatty Acid Composition of BeefFatty acid composition has become recognized as an important trait in beef because of its relationship with beef quality, including beef flavor and tenderness. Our laboratory attempted to identify genes responsible for the fatty acid composition of cattle. We found several such genes and developed genetic markers of fatty acid composition. The findings were as follows. 1) Genetic polymorphisms of the stearoyl-CoA desaturase (SCD) gene are associated with fatty acid composition. The effects of SCD type A gene substitution on the percentage of monounsaturated fatty acid (MUFA) and the melting point of intramuscular fat were on average approximately +1.0% and -1.0 degrees C, respectively. 2) Intron polymorphisms of sterol regulatory element binding protein-1 (SREBP-1) also affected MUFA. 3) No effect of SCE) or SREBP-1 genotypes on any representative carcass traits of Japanese Black cattle was observed in the field population. 4) Additional genetic markers, adipocytes fatty acid binding protein 4 (FABP4) and liver X receptor a, also affected the fatty acid composition. 5) SCD and FABP4 significantly affected fatty acid composition of Holstein steers. These findings bring new insights into the fat-related carcass traits of beef cattle. In addition, the developed DNA makers will contribute to improved meat quality of beef.KARGER, Jan. 2012, FOOD SCIENCE AND TECHNOLOGY RESEARCH, 18(1) (1), 1 - 6, English[Refereed][Invited]Book review
- 01 Dec. 2011, 動物遺伝育種研究, 39(2) (2), 142, Japanese兵庫県黒毛和種肥育集団における経済形質関連遺伝子の遺伝構造
- 01 Dec. 2011, 動物遺伝育種研究, 39(2) (2), 133, Japaneseホルスタイン集団におけるsliding window approachを利用した乳形質関連遺伝子座の探索
- 01 Dec. 2011, 動物遺伝育種研究, 39(2) (2), 135, Japanese黒毛和種集団を用いた脂肪酸組成に関するゲノムワイド相関解析
- 01 Dec. 2011, 動物遺伝育種研究, 39(2) (2), 142, Japanese黒毛和種における各地域集団の遺伝的多様性と類縁関係の解析
- 全国和牛登録協会, Oct. 2011, 和牛, 257, 6 - 10, Japanese一塩基多型（SNP）を利用した和牛の遺伝子型解析[Invited]Introduction commerce magazine
- 肉牛新報社, Aug. 2011, 肉牛ジャーナル, 8, 25, Japanese牛肉の脂肪酸組成に関わる遺伝子とその効果[Invited]Introduction commerce magazine
- 日本食肉研究会, Jun. 2011, 食肉の科学, 52(1) (1), 7 - 12, Japaneseおいしい牛肉や牛肉偽装を見抜くDNA診断法の開発[Refereed][Invited]Introduction scientific journal
- The Korean Society of Poultry Science, Jun. 2011, Korean Journal of Poultry Science, 38(2) (2), 145 - 154, EnglishIdentification of the gene responsible for chicken muscular dystrophy[Refereed][Invited]Introduction scientific journal
- (一社)日本細胞生物学会, May 2011, 日本細胞生物学会大会講演要旨集, 63回, 149 - 149, Englishニワトリ筋ジストロフィーと関連があるWWP1遺伝子における点変異が、変異WWP1導入遺伝子を発現したマウス筋に与える影響(Effect of a Point Mutation in the WWP1 Gene Associated with Chicken Muscular Dystrophy on Mouse Muscle Expressing Mutated WWP1 Transgene)
- 畜産技術協会, Jan. 2011, 畜産技術, 668(1) (1), 29 - 32, Japanese黒毛和種の現場後代検定牛におけるSCDおよびSREBP遺伝子型の効果[Invited]Introduction commerce magazine
- The fatty acid composition of adipose tissue in beef has been recognized as an important trait because of its relationship with beef quality, including favorable beef flavor and tenderness. Over the last decade, we have tried to identify the genes responsible for the fatty acid composition in cattle, and have found the following. (i) Genetic polymorphism of stearoyl-CoA desaturase (SCD) is one of the responsible genes associated with fatty acid composition. The average effects of gene substitution of the SCD type A gene on the monounsaturated fatty acid (MUFA) percentage and the melting point of intramuscular fat were approximately +1.0% and -1.0 degrees C, respectively. (ii) Intron polymorphism of sterol regulatory element binding protein-1 (SREBP-1) also affected MUFA. (iii) No effect of SCD or SREBP-1 genotypes on any representative carcass traits of Japanese Black in the field population was observed. (iv) Additional genetic markers adipocytes fatty acid binding protein 4 (FABP4) and liver X receptor alpha also affected the fatty acid composition. (v) SCD and FABP4 significantly affected fatty acid composition in Holstein steers. These findings will bring new insight into the fat-related carcass traits of beef cattle and will thus contribute to the beef industry.WILEY-BLACKWELL PUBLISHING, INC, 2011, ANIMAL SCIENCE JOURNAL, 82(1) (1), 1 - 7, English[Refereed]Book review
- 国立民族学博物館, Dec. 2010, 月刊「みんぱく」, 12, 5, Japanese美味しい牛肉の探求と品種の多様性[Invited]Introduction research institution
- 関西実験動物研究会, Dec. 2010, 関西実験動物研究会会報, 32, 1 - 12, Japaneseニワトリ筋ジストロフィー責任遺伝子の同定とモデル動物としての展開[Invited]Introduction scientific journal
- Effects of bovine fatty acid synthase, stearoyl-CoA desaturase, sterol regulatory element-binding protein 1, and growth hormone gene polymorphisms on fatty acid composition and carcass traits in Japanese Black cattle筋肉内脂肪の質は牛肉の肉質を特徴づける重要な因子である。脂肪の質は含まれる各種脂肪酸の割合によって決定される。脂質代謝に関わる遺伝子の中で、脂肪酸合成酵素FASN、ステアリル酸-CoA不飽和化酵素SCD，ステロール調節配列結合蛋白質SREBP1、そして成長ホルモンGHの各遺伝子上にはそれぞれ1ヶ所以上の多型部位が報告されている。そこで本研究では黒毛和種肥育牛を用いて、胸最長筋内脂肪の脂肪酸組成や枝肉形質に対する4遺伝子上の5種類の遺伝子多型の単独効果とエピスタシス効果を調べた。その結果、FASN、SCD、そしてGH L127Vについて脂肪酸結成に対する単独効果が検出された。中でもSCD上に存在するA293V多型はミリスチン酸(C14:0，P＜0.001)、ミリストレイン酸(C14:1，P < 0.001)、ステアリン酸(C18:0，P < 0.001)、オレイン酸(C18:1，P ＜ 0.001)、そして一価不飽和脂肪酸(MUFA、P ＜ 0.001)について最も大きな効果を示した。FASN、SCD、GH L127Vの3遺伝子マーカーはC18:1割合に対する遺伝分散の20%以上を説明しており、それぞれの遺伝子マーカーが成績の低い遺伝子型から高い遺伝子型に換わった場合、C18:1割合は4.46%高くなると推定された。脂肪酸組成に対するエピスタシス効果は脂肪酸合成関連の3遺伝子(FASN、SCD、SREBP1) 中では、どの2遺伝子間でも見出されなかった。一方、同一染色体上に存在するFASN、SREBP1、GH L127Vの間では各々の効果内に連鎖する別の遺伝子の影響が認められた。FASN、SCD、そしてSREBP1は、枝肉形質のうち肉量形質に対しては単独効果が検出されなかった。FASNとSCDの間では、枝肉形質でもエピスタシス効果は認められなかった(P＞ 0.1)。以上の結果から、脂肪酸合成に関わる2遺伝子(FASNとSCD)は胸最長筋内脂肪の脂肪酸組成に対して独立に影響していること、一方C18:1の割合を高くする方向に2遺伝子の遺伝子型を選抜した場合にも枝肉形質に対しては負の影響を与えないことが示され、FASNとSCD上のSNPは黒毛和種の脂肪酸組成を改良する際に有効なDNAマーカーとなることが示唆された。岐阜県畜産研究所, Jul. 2010, Bulletin of the Gifu Prefectural Livestock Research Institute, 10(10) (10), 11 - 22, JapaneseReport research institution
- 日本動物遺伝育種学会, Jun. 2010, 動物遺伝育種研究, 38(1) (1), 21 - 28, Japanese[Refereed]Introduction scientific journal
- 2010, 日本畜産学会大会講演要旨, 112th黒毛和種における脂肪酸組成の遺伝的パラメータに対する遺伝子多型の影響
- (公社)日本畜産学会, Sep. 2009, 日本畜産学会大会講演要旨集, 111回, 41 - 41, Japaneseニワトリ筋ジストロフィーにおけるcaveolin-3の発現解析
- 関西畜産学会, Feb. 2009, 関西畜産学会報, 164, 37 - 43, Japanese牛肉の美味しさに関る遺伝子とその効果[Invited]Introduction scientific journal
- 05 Jun. 2008, 日本養豚学会誌 = The Japanese journal of swine science, 45(2) (2), 93 - 93, Japaneseバングラデシュにおける在来豚の飼養と形態の特徴 : 特に西側地域の集団について
- 01 Jun. 2008, 動物遺伝育種研究 = The journal of animal genetics, 36(1) (1), 3 - 3, Japanese最先端動物遺伝育種セミナーに参加してみませんか?
- 日本生物工学会, 2008, 生物工学会誌 : seibutsu-kogaku kaishi, 86(8) (8), 410 - 411, Japanese科学を用いた美味しさの追及「神戸大学ビーフ」(大学発!美味しいバイオ)
- 農林水産技術情報協会, 01 Oct. 2007, 農林水産技術研究ジャーナル = Research Journal of Food and Agriculture, 30(10) (10), 16 - 20, JapaneseDevelopment of breed identification markers in beef cattle
- 2007, Rep. Soc. Res. Native Livestock, 24, 167 - 178Composition of the genes controlling blood groups and morpho-genetic traits of Bhutanese native chickens and its phylogenetic study.
- GENETICS SOC JAPAN, Dec. 2006, GENES & GENETIC SYSTEMS, 81(6) (6), 424 - 424, EnglishSequencing and phylogenetic analysis of beta globin gene in cattleSummary international conference
- (公社)日本畜産学会, 20 Mar. 2006, 日本畜産学会大会講演要旨, 106th, 73 - 73, JapaneseF2家系を用いたニワトリ筋ジストロフィー原因遺伝子の探索
- 2006, 日本畜産学会大会講演要旨, 106thブータン王国における豚の飼育状況と遺伝的組成
- 2006, 動物遺伝育種研究, 34(2) (2)ブータン在来鶏における血液タンパク質多型の解析
- 20 Jun. 2005, 日本養豚学会誌 = The Japanese journal of swine science, 42(2) (2), 77 - 77, Japaneseカンボジアに現存する小耳種系豚 : その形態と飼養状況について
- 2005, 日本畜産学会大会講演要旨, 104thカンボジア在来牛の血液蛋白型支配遺伝子構成
- 2005, 日本畜産学会大会講演要旨, 104thSRY遺伝子とミトコンドリアDNAの多型を指標にしたカンボジア在来牛における北方系牛とインド系牛の遺伝的寄与の評価
- 2005, 日本畜産学会大会講演要旨, 104thカンボジア在来水牛のミトコンドリアDNA多型解析
- 2005, 日本畜産学会大会講演要旨, 104thmtDNAの解析に基づいたカンボジア在来ブタとカンボジア産イノシシの系統関係
- 2005, 日本畜産学会大会講演要旨, 104thカンボジア在来水牛の血液蛋白型支配遺伝子構成
- 2005, 日本畜産学会大会講演要旨, 104thカンボジアにおける在来家畜及び近縁野生種の遺伝資源学的調査研究(概要)
- 2005, 日本畜産学会大会講演要旨, 104thカンボジア在来山羊の遺伝子構成
- 2004, 日本畜産学会大会講演要旨, 103rdミャンマー在来ブタとミャンマー産イノシシの系統関係
- 2004, 日本畜産学会大会講演要旨, 103rdSRY遺伝子とミトコンドリアDNAの多型を指標にしたミャンマー在来牛における北方系牛とインド系牛の遺伝的寄与の評価
- 2004, 日本家禽学会誌, 41カンボジアおよびその近隣東南アジア諸国におけるセキショクヤケイおよび在来鳥の分子系統学的解析
- 2003, 日本畜産学会大会講演要旨, 101stミャンマー産野生イノシシおよび在来豚の核型解析
- 2003, 日本畜産学会大会講演要旨, 101stミャンマーの在来家畜に関する研究 2. 在来牛の血液蛋白型支配遺伝子構成
- 2003, 日本畜産学会大会講演要旨, 101stミャンマーの在来家畜に関する研究 3. 在来水牛の血液蛋白型支配遺伝子構成
- 16 Jan. 2002, 日本胚移植学雑誌 = Japanese journal of embryo transfer, 24(1) (1), 28 - 33, Japanese黒毛和種の産肉形質に対するミトコンドリア DNA 効果
- 日本畜産学会, 25 Oct. 2000, Animal science journal, 71(10) (10), J470 - J474, JapaneseMitochondrial DNA Diversity of Japanese Black Cattle
- 25 Oct. 2000, 日本畜産學會報 = The Japanese journal of zootechnical science, 71(10) (10), J470 - J474, JapaneseMitochondrial DNA Diversity of Japanese Black Cattle
- 25 May 2000, 日本畜産學會報 = The Japanese journal of zootechnical science, 71(3) (3), 231 - 238, EnglishGenetic Analysis of Chicken Lines by Amplified Fragment Length Polymorphism (AFLP)
- 2000, 日本畜産学会大会講演要旨, 97thSRY遺伝子の多型解析を用いたラオス在来牛の系統的位置づけ
- 2000, 日本畜産学会大会講演要旨, 97thラオス在来ブタにおけるミトコンドリアDNAのRFLP解析
- 1999, 日本畜産学会大会講演要旨, 96thラオス在来牛の血液蛋白型支配遺伝子構成とその系統遺伝学的研究
- 1999, 日本畜産学会大会講演要旨, 96thニワトリMHC Classll Y-LβIII遺伝子を指標としたラオス在来鶏の分子系統学的解析
- 1999, 日本畜産学会大会講演要旨, 96thラオス在来山羊の遺伝子構成
- 1999, 日本畜産学会大会講演要旨, 96thウシ・ヘモグロビンβ鎖の多型におけるβグロビン遺伝子のDNA塩基配列の比較解析
- 1999, 日本畜産学会大会講演要旨, 95thラオス在来豚の飼養状況と形態および遺伝的特徴
- 1999, 日本畜産学会大会講演要旨, 95thmtDNAを用いたラオス在来ヤギの多型解析とその起源
- 1999, 日本畜産学会大会講演要旨, 95thラオス在来牛におけるmtDNA・D-loop領域の多型解析
- 1999, 日本畜産学会大会講演要旨, 96thラオス在来水牛の血液蛋白型支配遺伝子構成とその系統遺伝学的研究
- 1997, 全国和牛登録協会会誌, 200, 39 - 49黒毛和種牛のミトコンドリアDNA D-loop 領域の多型と枝肉成績との関係
- 日本万国家禽学会, Jan. 1993, 日本家禽学会誌, 30(1) (1), p66 - 71, Japanese体重選抜ウズラ系統におけるDNAフィンガ-プリント像による遺伝的構造の分析
- 1993, J.Anim.Genet., 21, 62 - 68DNA fingerprinting for individual identification and parentage test in Japanese Black cattle using five different mini-and on emicro-satellite probes
- 1991, ABRI, 19, 39 - 44ブタにおけるDNAフィンガープリント法の有用性
- 1991, ABRI, 19, 39 - 44Usufulness of DNA fingerprinting for Swine
- Contributor, 品種 pp.18-23, 緑書房, Mar. 2021新しい子牛の科学 -胎生期から初産分娩まで- 家畜感染症学会編
- Editor, 東京農業大学出版社, Aug. 2020アジアの在来家畜 -写真からみえる半世紀の記録-
- Joint editor, 兵庫県農政環境部農林水産局畜産課, Mar. 2018, Japanese兵庫の和牛 但馬牛 第6章General book
- Joint editor, 株式会社朝倉書店, Mar. 2017, JapaneseAnimal Breeding and GeneticsTextbook
- Others, 朝倉書店, May 2014, Japanese獣医遺伝育種学「家畜の品種と遺伝的多様性」Textbook
- Others, 朝倉書店, May 2014, Japanese獣医遺伝育種学「応用分子遺伝学とその実践」Textbook
- Others, 朝倉書店, Nov. 2013, Japaneseウシの科学「ウシの起源と品種」Textbook
- Others, 朝倉書店, Nov. 2013, Japaneseウシの科学「ウシの遺伝」Textbook
- Others, 文永堂出版, Jun. 2012, Japanese畜産学入門「遺伝と育種」Textbook
- Others, ㈱シーエムシー出版, May 2011, Japanese食のバイオ計測の最前線―機能解析と安全・安心の計測を目指して―「DNA鑑定を利用した牛肉偽装表示の防止」Scholarly book
- Others, 昭和堂, Apr. 2011, Japanese農村で学ぶはじめの一歩 -動物・在来家畜から畜産-Textbook
- Joint editor, 日本分析化学 表示･期限分析技術研究懇談会編, Nov. 2010, Japanese食品表示を裏づける分析技術 科学の目で偽装を見破る 「品種鑑別 牛」Scholarly book
- Joint work, 名古屋大学出版会, Nov. 2009, Japaneseアジアの在来家畜Scholarly book
- Joint work, チクサン出版社,緑書房(発売), Jan. 2008, Japanese, ISBN: 9784885006548新獣医学辞典
- Joint work, 17. Duality in Bos indicus mtDNA Diversity: Support for Geographical Complexity in Zebu Domestication, pp385-391, Springer, 2007The Evolution and History of Human Populations in South Asia -Inter-disciplinary Studies in Archaeology, Biological Anthropology, Linguistics and Genetics-
- Joint work, 国内産牛肉のDNA品種鑑定法（P356-365）, 光琳, Nov. 2006, Japanese, ISBN: 4771296049新・食品分析法
- Joint work, サイエンスフォーラム，東京, 2005, Japanese生鮮食肉の品種、鑑別食品鑑定技術ハンドブック，87-94General book
- Joint work, 636, 社団法人 畜産技術協会, Mar. 2001動物遺伝育種学事典
- Joint translation, ミトコンドリアDNA変異と細胞質遺伝効果（P196-199), ミトコンドリアDNA D-loop領域の分析でウシ品種の成立過程や相互関係がわかる（P199-202), 社団法人 畜産技術協会, Mar. 2000家畜ゲノム解析と新たな家畜育種戦略
- Joint work, DNAフィンガープリント法（P51-52）・遺伝子の単離と塩基配列の解析(P104-117), 朝倉書店, Mar. 1998, Japanese, ISBN: 4254450168動物遺伝育種学実験法
- Joint work, 188-190, チクサン出版社, Apr. 1995, Japanese, ISBN: 488500411Xアニマルバイオテクノロジー : 獣医・畜産研究者のための基礎知識
- 日本動物遺伝育種学会第25回大会, Nov. 2024, Japaneseウシ6番染色体の脂肪酸組成QTLに対する責任多型の探索
- 日本動物遺伝育種学会第25回大会, Nov. 2024, Japaneseミトゲノム解析による東南アジア在来ヤギの伝播過程の推定
- Plant and Animal Genome 31 Conference, Jan. 2024, EnglishExploring the gene polymorphisms associated with high-altitude adaptation in Nepalese goat breeds using Whole-genome resequencing
- Plant and Animal Genome 31 Conference, Jan. 2024, EnglishThe reported indel on caprine chromosome 1 is responsible for polledness in Asian goats
- Plant and Animal Genome 31 Conference, Jan. 2024, JapaneseComprehensive verification of all SNPs within a QTL region suggested that ICAM gene polymorphisms would be responsible for beef marbling in Japanese Black cattle
- 日本動物遺伝育種学会第24回大会, Nov. 2023全ゲノムリシーケンスデータを用いたヤギ高地適応遺伝子多型の探索
- 日本動物遺伝育種学会第24回大会, Nov. 2023兵庫県黒毛和種集団を用いた17番染色体における脂肪交雑QTLの探索
- 日本動物遺伝育種学会第24回大会, Nov. 2023, Japaneseミトゲノム解析によるアジア在来ヤギハプログループBの起源および伝播経路の推定
- 第131回日本畜産学会大会, Sep. 2023アジア在来ヤギにおける毛色の多様性に関わるMC1R遺伝子多型の探索
- 第131回日本畜産学会大会, Sep. 2023高密度 SNPマーカーを用いた東南アジア諸島在来ヤギの遺伝構造と伝播、遺伝子流入の推定
- 第131回日本畜産学会大会, Sep. 2023宮崎県黒毛和種集団を用いたウシ7番染色体における脂肪交雑に対する原因多型の探索
- Plant and Animal Genome 30 Conference, Jan. 2023Verification of candidate SNP effects reveals two QTLs on BTA7 for beef marbling in two Japanese Black cattle populations
- Plant and Animal Genome 30 Conference, Jan. 2023"Genome-Wide Association Study for the Percentage of Oleic Acid in Japanese Black Cattle Revealed the Candidate Region on BTA19"
- 第23回日本動物遺伝育種学会大会, Nov. 2022岩手県黒毛和種における白斑とMITF遺伝子多型との関連
- 第130回日本畜産学会大会, Sep. 2022兵庫県黒毛和種における ゲノムワイド関連解析を用いた オレイン酸含有率QTLの探索
- WCGALP congress, Jul. 2022Y-chromosomal haplogroups from wild and domestic goats reveal ancient migrations and recent introgressions"
- Plant and Animal Genome XXIX Conference, Jan. 2022Unravelling the Historical Biogeography of Philippine Domestic Pigs and Wild Pigs Inferred from Mitochondrial DNA
- 第22回日本動物遺伝育種学会大会, Nov. 2021父母系および核ゲノムSNPマーカーを用いたインドネシア在来ヤギの遺伝構造解析及び遺伝子流入の推定
- 第22回日本動物遺伝育種学会大会, Nov. 2021アジア在来ヤギにおける角の有無と既報の1番染色体無角関連多型との関連
- The 4th International Conference of Animal Science and Technology, Nov. 2021, EnglishOrigins and propagation routes of South/Southeast Asian goats analyzed by DNA information[Invited]Keynote oral presentation
- 第38回農薬環境科学研究会, Oct. 2021, Japanese神戸ビーフ：その美味しさと起源を探る -分子遺伝学からのアプローチ-[Invited]Invited oral presentation
- 第128回日本畜産学会大会, Mar. 2021, Japanese黒毛和種肥育牛における脂肪酸組成およびアミノ酸含量の遺伝的評価の試みPoster presentation
- 第128回日本畜産学会大会, Mar. 2021, Japanese父母系および高密度 SNPマーカーを用いたフィリピン在来ヤギの遺伝構造と伝播の推定Oral presentation
- 第128回日本畜産学会大会, Mar. 2021, Japanese50K SNPアレイを用いた和牛4品種に関する多様性および類縁関係の調査Oral presentation
- 第21回日本動物遺伝育種学会大会, Nov. 2020, Japanese集積流体回路を用いた兵庫県黒毛和種集団におけるロース脂肪割合候補多型の効果の検証Oral presentation
- 第21回日本動物遺伝育種学会大会, Nov. 2020, Japaneseネパール在来ヤギにおけるEPAS1, FGF5遺伝子多型と高地適応形質との関連調査Oral presentation
- 第20回日本動物遺伝育種学会大会, Nov. 2019, Japaneseミトゲノム配列による ハプログループT4の系統解析Oral presentation
- 第20回日本動物遺伝育種学会大会, Nov. 2019, Japanese在来鶏および改良品種の遺伝的構造と遺伝的多様性に関する研究Oral presentation
- The 10th anniversary Kobe University Brussels European Centre Symposium, Oct. 2019, EnglishIdentification and utilization of responsible genes associated with beef quality in Japanese Wagyu[Invited]Invited oral presentation
- Livestock Breeding and Genetics in Asian Countries, Hassanudin University, Oct. 2019, EnglishAnimal Breeding & Genetics in Japan and Japanese Wagyu[Invited]Keynote oral presentation
- 日本短角種研究会岩手大会, Sep. 2019, Japaneseゲノム情報に基づく日本短角種の遺伝的構造と起源・伝播[Invited]Keynote oral presentation
- 37th International Society for Animal Genetics Conference, Jul. 2019, EnglishPhylogenetic analysis of Nepalese goats using mtDNA D-loop region and SRY gene sequencesPoster presentation
- 37th International Society for Animal Genetics Conference, Jul. 2019, EnglishGenetic diversity of Bos taurus and Bos indicus using developed indel markersPoster presentation
- 37th International Society for Animal Genetics Conference, Jul. 2019, EnglishGenetic diversity and structure in 11 native Asian goat populations analyzed by high density SNP arrayPoster presentation
- 37th International Society for Animal Genetics Conference, Jul. 2019, EnglishValidation of candidate SNPs for fat percentage in bovine rib-eye area using Integrated Fluidic CircuitPoster presentation
- 37th International Society for Animal Genetics Conference, Jul. 2019, EnglishEffect of the PLAG1 gene polymorphism on carcass weight and oleic acid percentage in Japanese Black cattle populationsPoster presentation
- The 2nd Joint Meeting of Veterinary Science in East Asia, Apr. 2019, English, Seoul, Korea, International conferenceMolecular epidemiological study of bovine leukemia virus in Zebu cattle (Bos indicus)Oral presentation
- 第125回日本畜産学会大会, Mar. 2019, Japanese, 日本畜産学会, 相模原, Domestic conference複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討Oral presentation
- 第54回獣医疫学会学術集会, Mar. 2019, Japanese, 獣医疫学会, 東京, Domestic conferenceアジア在来牛における牛白血病ウイルスの分子疫学的解析Oral presentation
- 第125回日本畜産学会大会, Mar. 2019, Japanese, 日本畜産学会, 相模原, Domestic conferencemtDNA D-loop領域とSRY遺伝子の塩基配列に基づいたネパール在来ヤギの遺伝的類縁関係Oral presentation
- Korean Society of Animal Breeding and Gnetics, 2019, EnglishIdentification & utilization of responsible genes associated with economic traits in Japanese Black cattle[Invited]Invited oral presentation
- 海洋水産資源開発事業（ブリ優良人工種苗周年供給システムの構築）に係るブリ育種勉強会, Dec. 2018, Japanese, 水産研究・教育機構 開発調査センター, 横浜, Domestic conference家畜ウシ（黒毛和種）の育種改良とDNA情報の応用と利用[Invited]Invited oral presentation
- 第18回日本動物遺伝育種学会大会, Sep. 2018, Japanese, 京都, Domestic conference兵庫県黒毛和種集団における全ゲノムリシーケンスデータを用いた枝肉形質に対する候補変異の抽出Oral presentation
- 第18回日本動物遺伝育種学会大会, Sep. 2018, Japanese, 京都, Domestic conferenceゲノムリシーケンスデータを用いたウシ10番染色体におけるロース脂肪割合の原因変異の探索Oral presentation
- 第18回日本動物遺伝育種学会大会, Sep. 2018, Japanese, 京都, Domestic conferenceカザフスタン在来ヤギに対するmtDNAとY染色体の塩基配列解析～Eurasian Steppeは重要なヤギの伝播経路の一つである～Oral presentation
- General Lecture in NARC & Emerald Technical College, Sep. 2018, English, NARC & Emerald Technical College, Kathmandu, Nepal, International conferenceNative livestock & goat genetic diversity in Eurasia & Nepal[Invited]Invited oral presentation
- Workshop in Hassanudin University, Jun. 2018, English, Hasanuddin University, Makassr, Indonesia, International conferenceWriting Publication and DNA Research Technology in Animal Production[Invited]Invited oral presentation
- General Lecture in Hassanudin University, Jun. 2018, English, Hasanuddin University, Makassr, Indonesia, International conferenceDNA technology in animal breeding research and it's application for native livestock characterization[Invited]Invited oral presentation
- PAG Asia 2018, May 2018, English, Scherago International, Seoul, Korea, International conferenceEffect of SLC27A6 gene K81M polymorphism on fat percentage in rib-eye area in Japanese Black cattlePoster presentation
- PAG Asia 2018, May 2018, English, Scherago International, Seoul, Korea, International conferenceDetection of candidate polymorphisms in the QTL for oleic acid percentage on BTA9 based on whole-genome resequencing dataPoster presentation
- 第124回日本畜産学会大会, Mar. 2018, Japanese, 日本畜産学会, 東京, Domestic conference兵庫県黒毛和種集団におけるSLC27A6遺伝子多型のロース脂肪割合に対する効果の検討Oral presentation
- 第124回日本畜産学会大会, Mar. 2018, Japanese, 日本畜産学会, 東京, Domestic conferenceウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索Oral presentation
- 第124回日本畜産学会大会, Mar. 2018, Japanese, 日本畜産学会, 東京, Domestic conferencemtDNA塩基配列を用いたカザフスタン在来ウシの系統遺伝学的解析Oral presentation
- 第18回日本動物遺伝育種学会大会, Nov. 2017, Japanese, 動物遺伝育種学会, 厚木, Domestic conference兵庫県黒毛和種におけるゲノムワイド関連解析により同定されたVNN1遺伝子T66M多型のオレイン酸含有率に対する効果Oral presentation
- 第18回日本動物遺伝育種学会大会, Nov. 2017, Japanese, 動物遺伝育種学会, 厚木, Domestic conference複数の黒毛和種集団を用いたPLAG1遺伝子多型のオレイン酸含有率に対する効果の検討Oral presentation
- 第67回関西畜産学会大会, Sep. 2017, Japanese, 関西畜産学会, 大阪, Domestic conference北海道ホルスタインは希少mtDNAハプログループPを有するOral presentation
- 第67回関西畜産学会大会, Sep. 2017, Japanese, 関西畜産学会, 大阪, Domestic conferenceゲノムリシーケンスデータを利用したウシ7番染色体におけるロース脂肪割合に関わる遺伝子変異の探索Oral presentation
- 第67回関西畜産学会大会, Sep. 2017, Japanese, 関西畜産学会, 大阪, Domestic conferenceIndelマーカーを用いた家畜ウシ11集団に対する遺伝的構造および系統解析Oral presentation
- 36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conferencePLAG1 polymorphism (ss319607405) is associated with oleic acid percentage in Japanese Black cattlePoster presentation
- 36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conferencePhylogenetic analysis of Kazakhstani goats using mtDNA HV1 and SRY gene sequencesPoster presentation
- 36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conferenceGenome-wide association study revealed a candidate region for beef marbling on BTA7 in Japanese Black cattlePoster presentation
- 36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conferenceGenetic structure and relationships among 11 cattle populations using Indel markersPoster presentation
- 36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, International conferenceGenetic characterization of native horses in Myanmar and Laos using haplotypes of mitochondrial DNA and Y-chromosomal markersPoster presentation
- 36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conferenceGenetic characteristics of Kazakhstan native horsesPoster presentation
- 36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conferenceEffects of DNA markers associated with carcass traits and fatty acid composition on fertility traits in Japanese Black cowsPoster presentation
- 36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conferenceAssociation of VNN1 gene polymorphism with fatty acid composition in Japanese Black cattlePoster presentation
- PAG Asia 2017, May 2017, English, South Korea, International conferenceWhy Saiga antelope (Saiga tatarica) died unexpected and frequently in Kazakhstan?Poster presentation
- PAG Asia 2017, May 2017, English, South Korea, International conferenceGenetic diversity and immigration roots of East Asian cattle[Invited]Invited oral presentation
- 第122回日本畜産学会大会, Mar. 2017, Japanese, 日本畜産学会, 神戸, Domestic conferenceカザフスタン在来牛のマイクロサテライトDNA多型Oral presentation
- 第122回日本畜産学会大会, Mar. 2017, Japanese, 日本畜産学会, 神戸, Domestic conferenceカザフスタン在来家畜とその近縁野生種の遺伝資源学的国際協同調査研究の概要Oral presentation
- 第122回日本畜産学会大会, Mar. 2017, Japanese, 日本畜産学会, 神戸, Domestic conferenceカザフスタン在来ヤギのマイクロサテライトDNA多型Oral presentation
- 第122回日本畜産学会大会, Mar. 2017, Japanese, 日本畜産学会, 神戸, Domestic conferenceカザフスタンにおける在来馬調査の概要Oral presentation
- 第122回日本畜産学会大会, Mar. 2017, Japanese, 日本畜産学会, 神戸, Domestic conferencemtDNA配列を用いたカザフスタン5地域の在来ヤギにおける遺伝的構造の比較Oral presentation
- 第17回日本動物遺伝育種学会大会, Nov. 2016, Japanese, 日本動物遺伝育種学会, 名古屋, Domestic conference黒毛和種繁殖雌牛集団におけるレプチン遺伝子内多型と繁殖形質との関連Oral presentation
- 第17回日本動物遺伝育種学会大会, Nov. 2016, Japanese, 日本動物遺伝育種学会, 名古屋, Domestic conference見島牛・口之島牛および黒毛和種のウシ主要組織適合遺伝子領域のリシークエンスによる比較解析Oral presentation
- 第17回日本動物遺伝育種学会大会, Nov. 2016, Japanese, 日本動物遺伝育種学会, 名古屋, Domestic conferenceカザフスタン在来馬の遺伝学的解析Oral presentation
- 第25回日本組織適合性学会大会, Oct. 2016, Japanese, 札幌, Domestic conference日本在来種と黒毛和種のウシMHC領域のリシークエンスによる比較解析Oral presentation
- The 1st Symposium in Jeju Black cattle Research Center: Genetics & Breeding and Mass Production of Domestic Animal using Genome Analysis, Oct. 2016, English, Jeju Island, Korea, International conferenceThe origin, immigration roots and genetic diversity of Japanese Wagyu[Invited]Invited oral presentation
- 第2回日本筋学会学術集会, Aug. 2016, Japanese, 東京, Domestic conference筋ジストロフィーモデル動物，NH-413ニワトリの骨格筋におけるWWP1タンパク質の分解と機能の喪失Oral presentation
- 17th AAAP Animal Science Congress, Aug. 2016, English, Fukuoka, Japan, International conferenceThe origin and genetic diversity of Japanese WagyuKeynote oral presentation
- 35th International Society for Animal Genetics Conference, Jul. 2016, English, International Society for Animal Genetics, Slat Lake City, USA, International conferenceIdentification of SNP associated with fertility trait using pool-based genome-wide association study in Japanese Black cattlePoster presentation
- 35th International Society for Animal Genetics Conference, Jul. 2016, English, International Society for Animal Genetics, Slat Lake City, USA, International conferenceIdentification of polymorphisms associated with oleic acid percentage by pool-based genome wide association study (GWAS) in Japanese BlackPoster presentation
- 35th International Society for Animal Genetics Conference, Jul. 2016, English, International Society for Animal Genetics, Slat Lake City, USA, International conferenceGenome-wide association study identifies a QTL for fat percentage in rib-eye area on BTA10 in Japanese Black cattlePoster presentation
- 35th International Society for Animal Genetics Conference, Jul. 2016, English, International Society for Animal Genetics, Slat Lake City, USA, International conferenceGenetic diversity and origin of mtDNA haplogroup P observed in Japanese ShorthornPoster presentation
- 第121回日本畜産学会大会, Mar. 2016, Japanese, 日本畜産学会, 東京, Domestic conference日本短角種において観察される希少なmtDNAハプログループPの母系起源Oral presentation
- 第121回日本畜産学会大会, Mar. 2016, Japanese, 日本畜産学会, 東京, Domestic conferenceウシ10番染色体におけるロース脂肪割合に影響する遺伝子の探索Poster presentation
- American Society for Cell Biology, Dec. 2015, English, San Diego, CA, International conferenceChanges in stability and subcellular localization of WWP1 protein in skeletal muscle of muscular dystrophy chickensOral presentation
- 第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference兵庫県黒毛和種集団におけるオレイン酸含有率に対するゲノムワイド関連解析Poster presentation
- 第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference黒毛和種における産肉形質関連マーカーの繁殖形質に対する効果の推定Poster presentation
- 第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference岐阜県黒毛和種の繁殖形質を対象としたゲノムワイド関連解析Poster presentation
- 第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conferenceGWASを用いた黒毛和種集団におけるロース脂肪割合に関わる遺伝子座の探索Poster presentation
- 第118回日本畜産学会大会, Mar. 2015, Japanese, 日本畜産学会, 宇都宮, Domestic conference黒毛和種集団におけるLeptin遺伝子翻訳領域内多型の脂肪酸組成に対する効果Oral presentation
- 第118回日本畜産学会大会, Mar. 2015, Japanese, 日本畜産学会, 宇都宮, Domestic conference高密度SNPアレイによる口之島牛の遺伝的多様性解析Oral presentation
- 第15回日本動物遺伝育種学会大会, Nov. 2014, Japanese, 日本動物遺伝育種学会, 和光, Domestic conference口之島牛のウシ主要組織適合遺伝子複合体領域の(MHC) 領域のゲノム多様性Poster presentation
- 第15回日本動物遺伝育種学会大会, Nov. 2014, Japanese, 日本動物遺伝育種学会, 和光, Domestic conferenceミャンマー在来牛を用いたBos taurus 由来DNAマーカーの遺伝子型判定Poster presentation
- 第15回日本動物遺伝育種学会大会, Nov. 2014, Japanese, 日本動物遺伝育種学会, 和光, Domestic conferenceホルスタイン種におけるFASN-g.841 及びg.16024 多型の乳形質に対する効果Poster presentation
- 第15回日本動物遺伝育種学会大会, Nov. 2014, Japanese, 日本動物遺伝育種学会, 和光, Domestic conferenceウシ主要組織適合遺伝子複合体領域(MHC) のターゲットゲノム解析Poster presentation
- 第15回日本動物遺伝育種学会大会, Nov. 2014, Japanese, 日本動物遺伝育種学会, 和光, Domestic conferenceSRY 遺伝子とmtDNA 塩基配列を用いたシバヤギの遺伝的多様性解析Poster presentation
- 第15回日本動物遺伝育種学会大会, Nov. 2014, Japanese, 日本動物遺伝育種学会, 和光, Domestic conferenceDigiTag2 assay を用いたアジア牛集団に対する遺伝的類縁関係と遺伝的構造の解析Poster presentation
- 第15回日本動物遺伝育種学会大会, Nov. 2014, Japanese, 日本動物遺伝育種学会, 和光, Domestic conference50K SNP チップを用いた口之島野生化牛の遺伝的多様性に関する研究Poster presentation
- 34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conferenceThe g.841G>C SNP of FASN gene is associated with fatty acid composition in beef cattleOral presentation
- 34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conferencePaternal genetic structure of Asian goats using SRY geneOral presentation
- 34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conferenceLPL gene polymorphisms are associated with fatty acid composition in Japanese BlackOral presentation
- 34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conferenceGenome wide association study revealed candidate regions for calving interval in Japanese black cattleOral presentation
- 34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conferenceGenetic diversity and relationship among 16 Asian and European cattle populations using 121 autosomal SNPs genotypes by the DigiTag2 assayOral presentation
- 34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conferenceA genome wide association study for beef marbling and fatty acid composition in Japanese Black cattle using pooled DNAOral presentation
- 北信越畜産学, Nov. 2013, Japanese, 金沢, Domestic conference牛肉品質に関わる遺伝子の同定と利用[Invited]Invited oral presentation
- 農林水産消費安全技術センター神戸センターおよび食の安全・安心科学センター合同発表会, Nov. 2013, Japanese, 神戸, Domestic conferenceSNPを用いたウシ品種鑑定法の概要と今後の課題Keynote oral presentation
- Diversifying Biological Resources〜Toward Food Security and Sustainable Society〜, Nov. 2013, English, Tsukuba, International conferenceCattle genetic diversity and breed identification based on DNA markers[Invited]Nominated symposium
- 第14回日本動物遺伝育種学会大会, Oct. 2013, Japanese, 東京, Domestic conference黒毛和種における脂肪酸割合に対するFASN、SCD、SREBP-1、GH遺伝子の影響および枝肉成績に対するNCAPG（CW-2）、GH、GHR遺伝子の影響Oral presentation
- 第14回日本動物遺伝育種学会大会, Oct. 2013, Japanese, 日本動物遺伝育種学会, 東京, Domestic conference黒毛和種におけるLEP遺伝子プロモーター領域に対する多型探索Poster presentation
- 第14回日本動物遺伝育種学会大会, Oct. 2013, Japanese, 東京, Domestic conference口之島牛における各種遺伝子の対立遺伝子頻度の調査Oral presentation
- 第14回日本動物遺伝育種学会大会, Oct. 2013, Japanese, 日本動物遺伝育種学会, 東京, Domestic conferencemtDNA塩基配列を用いた口之島野生化牛、日本短角種、無角和種の遺伝的多様性解析Poster presentation
- 香川県獣医師会, Sep. 2013, Japanese, 香川, Domestic conference讃岐牛の改良に貢献する美味しさ関連遺伝子(DNAマーカー)の探索Oral presentation
- 第116回日本畜産学会大会, Mar. 2013, Japanese, 日本畜産学会, 広島, Domestic conferenceウシ脂肪酸組成に対するFASN 遺伝子内多型の効果Oral presentation
- 第116回日本畜産学会大会, Mar. 2013, Japanese, 日本畜産学会, 広島, Domestic conferenceSRY遺伝子を用いたアジア在来家畜ヤギの多様性解析Oral presentation
- 安全安心な畜産物の輸出促進に向けて～ＤＮＡ検査技術の活用可能性～, Feb. 2013, Japanese, 宮崎, Domestic conference畜産業における遺伝子検査の現状Public discourse
- DNA検査技術を活用した農畜産物の「食の安心・安全」公開検討会, Feb. 2013, Japanese, 沖縄, Domestic conference畜産業における遺伝子検査の現状Public discourse
- くまもとバイオ＆ライフサイエンスカフェ, Feb. 2013, Japanese, 熊本, Domestic conference食の安心安全の高度化と知財保護の必要性～優良品種と識別技術～Public discourse
- 神戸大学のミリョク, Feb. 2013, Japanese, 東京, Domestic conferenceDNAでひも解く牛肉のおいしさと起源Public discourse
- 千里ライフサイエンスフォーラム, Jan. 2013, Japanese, 大阪, Domestic conference和牛のおいしさと起源 - DNAで牛肉の品質や素性を探る –[Invited]Nominated symposium
- ＤＮＡチップ技術を活用した「食の安心・安全」公開検討会, Dec. 2012, Japanese, 十勝, Domestic conference畜産業における遺伝子検査の現状Public discourse
- 日本食品工業倶楽部月例特別講演, Nov. 2012, Japanese, 大阪, Domestic conference牛肉の品種・産地偽装とDNA鑑定[Invited]Invited oral presentation
- 第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 日本動物遺伝育種学会, 仙台, Domestic conference黒毛和種の枝肉形質に対するSCD、SREBP1、FASN およびNCAPG 遺伝子多型の関連解析Poster presentation
- 第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 日本動物遺伝育種学会, 仙台, Domestic conference黒毛和種におけるLEP 遺伝子内多型の脂肪酸組成に対する効果Poster presentation
- 第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 日本動物遺伝育種学会, 仙台, Domestic conference黒毛和種におけるFASN 遺伝子内多型の脂肪酸組成に対する効果Poster presentation
- 第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 日本動物遺伝育種学会, 仙台, Domestic conference岐阜県黒毛和種集団におけるUTS2R 遺伝子内多型の脂肪酸組成に対する効果Poster presentation
- 第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 仙台, Domestic conferenceウシFABP4 遺伝子のプロモーター領域に対する多型探索Poster presentation
- 神戸大学農学部ホームカミングデイ受賞講演, Oct. 2012, Japanese, 神戸, Domestic conferenceアジア在来家畜の現地調査と家畜牛の起源[Invited]Invited oral presentation
- 33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conferenceUTS2R gene polymorphisms are associated with fatty acid composition in Japanese beef cattlePoster presentation
- 33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conferenceThe SNPs in the ACACA gene effective on fatty acid composition in Holstein milkPoster presentation
- 33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conferenceThe SNP in the promoter region of the bovine ELOVL5 gene is effective on subcutaneous fat thicknessPoster presentation
- 33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conferenceIdentification of quantitative trait loci affecting economic traits based on divergently selected regions between Japanese Black and Holstein cattlePoster presentation
- 33nd International Conference on Animal Genetics, Jul. 2012, English, Cairns, Australia, International conferenceGenotyping of genes responsible for 7 defective traits in Japanese Black by DigiTag2 assayPoster presentation
- 33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conferenceGenome wide association study for fatty acid composition in Japanese Black cattlePoster presentation
- 33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conferenceGenetic diversity and structure in goat analyzed by newly-developed SNPsPoster presentation
- 33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conferenceEvaluation of genetic diversity in Japanese Black cattle population using SNP-MaP strategyPoster presentation
- 日本動物遺伝育種学会・在来家畜研究会合同シンポジウム「和牛育種戦略の新展開－在来牛遺伝資源、遺伝的多様性および知的財産の活用－」, Mar. 2012, Japanese, 名古屋, Domestic conference分子遺伝学から見たアジア在来牛の起源・系統史研究[Invited]Nominated symposium
- 第115回日本畜産学会大会, Mar. 2012, Japanese, 名古屋, Domestic conferenceプーリング法を用いた高密度SNP解析による黒毛和種閉鎖集団の遺伝的多様性の評価Oral presentation
- 第５３回日本食肉研究会大会, Mar. 2012, Japanese, 名古屋, Domestic conferenceおいしい牛肉や牛肉偽装を見抜くDNA診断法の開発[Invited]Invited oral presentation
- 全国学校栄養士協議会, Feb. 2012, Japanese, 京都, Domestic conference牛肉の素性と品質を遺伝子で探る[Invited]Invited oral presentation
- 第62回関西畜産学会大会, 2012, Japanese, Domestic conference黒毛和種の肥育後期における胸最長筋および腰最長筋の脂肪酸組成の経時的変化Oral presentation
- 第12回日本動物遺伝育種学会大会, Nov. 2011, Japanese, 日本動物遺伝育種学会, 広島, Domestic conference兵庫県黒毛和種肥育集団における経済形質関連遺伝子の遺伝構造Poster presentation
- 第12回日本動物遺伝育種学会大会, Nov. 2011, Japanese, 日本動物遺伝育種学会, 広島, Domestic conference黒毛和種集団を用いた脂肪酸組成に関するゲノムワイド相関解析Poster presentation
- 第12回日本動物遺伝育種学会大会, Nov. 2011, Japanese, 日本動物遺伝育種学会, 広島, Domestic conference黒毛和種およびホルスタインにおけるUTS2R遺伝子多型の脂肪酸組成に対する効果Poster presentation
- 第12回日本動物遺伝育種学会大会, Nov. 2011, Japanese, 日本動物遺伝育種学会, 広島, Domestic conferenceホルスタイン集団におけるsliding window approachを利用した乳形質関連遺伝子座の探索Poster presentation
- The Role of Agriculture and Natural Resources on Global Changes, Nov. 2011, English, Chiang Mai, Thailand, International conferenceDevelopment of DNA markers associated with beef quality[Invited]Invited oral presentation
- 第61回関西畜産学会大会, Sep. 2011, Japanese, 関西畜産学会, 岡山, Domestic conference黒毛和種閉鎖集団における形質関連遺伝子頻度の経時的変化Oral presentation
- 第61回関西畜産学会大会, Sep. 2011, Japanese, 関西畜産学会, 岡山, Domestic conferenceウシELOVL5とELOVL6遺伝子のプロモーター領域に対する多型探索Oral presentation
- 第61回関西畜産学会大会, Sep. 2011, Japanese, 関西畜産学会, 岡山, Domestic conferenceDigiTag2 アッセイを用いたウシ個体識別及び親子鑑定システムの確立Oral presentation
- 地球研生存地イニシアティブ・花博協会共催シンポジウム, Mar. 2011, Japanese, 京都, Domestic conference和牛のルーツと多様性Keynote oral presentation
- 日本畜産学会第114回大会, 2011, Japanese, Domestic conference黒毛和種の肥育後期における腰最長筋の筋肉内脂肪および脂肪酸組成の経時的変化Poster presentation
- H23全国和牛登録協会公開セミナー, Jan. 2011, Japanese, 京都, Domestic conference遺伝的多様性に係わるDNA（SNPs）情報の利用の可能性についてPublic discourse
- 「食品・農産物の表示の信頼性確保と機能性解明のための基盤技術の開発」研究成果発表会「信頼できる食品の表示の確保と健康増進に向けた食品の機能性の解明」, Nov. 2010, Japanese, つくば, Domestic conference輸入牛肉と国産牛肉をDNA診断で見分けるPublic discourse
- 第６回バイオ計測プロジェクト / 第４回バイオ計測・試薬研究会「健食同源に繋がる食の安全安心科学・技術の更なる発展を目指して」発表交流会, Nov. 2010, Japanese, 神戸, Domestic conference牛肉の偽装表示を防ぐ輸入牛肉に対するDNAマーカーの開発Keynote oral presentation
- 朝日新聞農学シンポジウム2010 「“食”から日本を盛り上げる」, Nov. 2010, Japanese, 大阪, Domestic conference遺伝子学で見破る牛肉の偽装表示[Invited]Nominated symposium
- 第11回日本動物遺伝育種学会大会, Oct. 2010, Japanese, 日本動物遺伝育種学会, 白河, Domestic conference黒毛和種集団におけるsliding window approachを利用した枝肉形質関連遺伝子座の探索Poster presentation
- 神戸大学農学部ホームカミングデイ講演, Oct. 2010, Japanese, 神戸, Domestic conference牛肉の品質と素性を遺伝子で探る －美味しさの秘密と偽装を防ぐ鑑定法－Public discourse
- 国産牛肉と外国産牛肉（豪州産および米国産）の判別法の妥当性確認のための共同試験の事前説明会, Oct. 2010, Japanese, 神戸, Domestic conference外国産輸入牛肉に対する判別DNAマーカーの開発Public discourse
- 第11回日本動物遺伝育種学会大会, Oct. 2010, Japanese, 日本動物遺伝育種学会, 白河, Domestic conferenceウシELOVL遺伝子の塩基配列決定及び多型探索Poster presentation
- RIHN 5th International Symposium "The past and future of diversity", Oct. 2010, Japanese, Kyoto, International conferenceCattle biodiversity in past and present in South to East Asia[Invited]Nominated symposium
- 32th International Conference on Animal Genetics, Jul. 2010, English, International Society for Animal Genetics, Edinburgh, UK, International conferencePolymorphisms of fat metabolism related genes and association between genotypes and carcass traits in Japanese Black cattlePoster presentation
- 32th International Conference on Animal Genetics, Jul. 2010, English, International Society for Animal Genetics, Edinburgh, UK, International conferenceIdentification of divergently selected regions between Japanese Black and Holstein cattle based on a bovine 50k SNP arrayPoster presentation
- 32th International Conference on Animal Genetics, Jul. 2010, English, International Society for Animal Genetics, Edinburgh, UK, International conferenceGenetic diversity and structure in Bos taurus and Bos indicus populations analyzed by SNP markersPoster presentation
- 32th International Conference on Animal Genetics, Jul. 2010, English, International Society for Animal Genetics, Edinburgh, UK, International conferenceDevelopment of discrimination markers between Japanese domestic and imported beef based on a bovine 50K SNP arrayPoster presentation
- 32th International Conference on Animal Genetics, Jul. 2010, English, International Society for Animal Genetics, Edinburgh, UK, International conferenceAllele frequencies of gene polymorphisms related to economic traits in Bos taurus and Bos indicus cattle breedsPoster presentation
- 神戸大学・兵庫県環境研究センター学術交流会, Mar. 2010, Japanese, 神戸, Domestic conference美味しい牛肉や偽装表示を見抜くDNA鑑定法の開発Public discourse
- 第112回日本畜産学会大会, Mar. 2010, Japanese, 東京, Domestic conference黒毛和種における脂肪酸組成の遺伝的パラメータに対する遺伝子多型の影響Oral presentation
- 第112回日本畜産学会大会, Mar. 2010, Japanese, 日本畜産学会, 明治大学, Domestic conference黒毛和種におけるDGAT1遺伝子多型の枝肉形質に対する効果Oral presentation
- 日本畜産学会賞受賞講演, Mar. 2010, Japanese, Domestic conference牛肉品質に関連する遺伝子の同定と利用[Invited]Invited oral presentation
- 第112回日本畜産学会大会, Mar. 2010, Japanese, 東京, Domestic conferenceバングラデシュにおける在来家畜及び野生原種の遺伝資源学的研究 ８．イノシシ型在来ブタの分布と肉髯Oral presentation
- 第112回日本畜産学会大会, Mar. 2010, Japanese, 東京, Domestic conferenceバングラデシュにおける在来家畜及び野生原種の遺伝資源学的研究 5.ヤギのマイクロサテライトDNA多型Oral presentation
- 第112回日本畜産学会大会, Mar. 2010, Japanese, 東京, Domestic conferenceバングラデシュにおける在来家畜及び野生原種の遺伝資源学的研究 3.スイギュウのmtDNAとマイクロサテライトDNA多型Oral presentation
- 第112回日本畜産学会大会, Mar. 2010, Japanese, 東京, Domestic conferenceバングラデシュにおける在来家畜及び野生原種の遺伝資源学的研究 2.輸入インド牛と在来牛の遺伝的類縁関係Oral presentation
- 第112回日本畜産学会大会, Mar. 2010, Japanese, 日本畜産学会, 明治大学, Domestic conferenceバングラデシュにおける 在来家畜及び野生原種の遺伝資源学的研究Oral presentation
- 第112回日本畜産学会大会, Mar. 2010, Japanese, 東京, Domestic conferenceウシHTR1B遺伝子コード領域内に存在する３つのＳＮＰの頻度分布Oral presentation
- 第112回日本畜産学会大会, Mar. 2010, Japanese, 日本畜産学会, 明治大学, Domestic conferenceSNPマーカーを用いたウシの遺伝的多様性と遺伝構造解析Oral presentation
- DNA考古学研究会, Feb. 2010, Japanese, 京都, Domestic conferenceDNAマーカーに基づくウシ属の分類と新疆ウィグル自治区・小河墓遺跡から出土した動物遺存体への適用[Invited]Nominated symposium
- 第2回DNA鑑定学会, Dec. 2009, Japanese, 横浜, Domestic conferenceウシにおける品種鑑定法の開発[Invited]Invited oral presentation
- 第10回日本動物遺伝育種学会大会, Nov. 2009, Japanese, 日本動物遺伝育種学会, 群馬会館, Domestic conferenceホルスタイン種におけるFASN遺伝子の多型探索及び乳形質の効果Poster presentation
- 第10回日本動物遺伝育種学会大会, Nov. 2009, Japanese, 日本動物遺伝育種学会, 群馬会館, Domestic conferenceBovine 50k SNPs情報を利用したウシゲノム解析Poster presentation
- 第111回日本畜産学会大会, Sep. 2009, Japanese, 沖縄, Domestic conference日本短角種牛肉における脂肪酸組成関連遺伝子多型の形質への関与Oral presentation
- 第111回日本畜産学会大会, Sep. 2009, Japanese, 日本畜産学会, 琉球大学, Domestic conference国産牛と米国産牛とのＤＮＡ鑑定マーカーの開発Oral presentation
- 第111回日本畜産学会大会, Sep. 2009, Japanese, 日本畜産学会, 琉球大学, Domestic conferenceホルスタイン種における枝肉形質に関る遺伝子多型の効果Oral presentation
- 関西実験動物学会, Sep. 2009, Japanese, 京都, Domestic conferenceニワトリ筋ジストロフィー責任遺伝子の同定とモデル動物としての展開Keynote oral presentation
- 第111回日本畜産学会大会, Sep. 2009, Japanese, 日本畜産学会, 琉球大学, Domestic conferenceニワトリ筋ジストロフィーにおけるcaveolin-3の発現解析Oral presentation
- 第111回日本畜産学会大会, Sep. 2009, Japanese, 日本畜産学会, 琉球大学, Domestic conferenceウシ疾病関連遺伝子、抗病性関連遺伝子の多様性解析Oral presentation
- 名古屋大学鳥類バイオサイエンス研究センター講演, Jun. 2009, Japanese, 名古屋, Domestic conferenceニワトリ筋ジストロフィー責任遺伝子の同定とモデル動物としての利用Keynote oral presentation
- 第110回日本畜産学会大会, Mar. 2009, Japanese, 日本畜産学会, 日本大学, Domestic conference黒毛和種の個体識別および親子鑑定を目的としたSNPsパネルの開発Oral presentation
- 第110回日本畜産学会大会, Mar. 2009, Japanese, 神奈川, Domestic conference黒毛和種における胸最長筋内脂肪酸組成と脂肪酸合成関連遺伝子FASN・SCD・SREBPおよび成長ホルモン遺伝子多型との関連Oral presentation
- 第110回日本畜産学会大会, Mar. 2009, Japanese, 日本畜産学会, 日本大学, Domestic conferenceウシ経済形質関連遺伝子の多様性解析Oral presentation
- 第104回日本畜産学会大会. 要旨集103, 2005, Japanese, 不明, .東京., Domestic conference黒毛和種、褐毛和種および韓牛のMC1R遺伝子における対立遺伝子頻度の比較Oral presentation
- 日本動物遺伝育種学会大会第6 回大会. 要旨集99, 2005, Japanese, 不明, 札幌, Domestic conferenceミトコンドリアDNA を用いた褐毛和種の遺伝的多様性に関する研究Oral presentation
- 日本動物遺伝育種学会大会第6 回大会. 要旨集92., 2005, Japanese, 不明, 札幌, Domestic conferenceニホンウズラ連鎖地図に対する機能遺伝子のマッピング.Oral presentation
- 第104回日本畜産学会大会. 要旨集96, 2005, Japanese, 不明, .東京., Domestic conferenceカンボジア在来水牛の血液蛋白型支配遺伝子構成Oral presentation
- 第104回日本畜産学会大会. 要旨集95, 2005, Japanese, 不明, .東京., Domestic conferenceカンボジア在来水牛のミトコンドリアDNA多型解析Oral presentation
- 第104回日本畜産学会大会. 要旨集95, 2005, Japanese, 不明, .東京., Domestic conferenceカンボジア在来山羊の遺伝子構成Oral presentation
- 第104回日本畜産学会大会. 要旨集95, 2005, Japanese, 不明, .東京., Domestic conferenceカンボジア在来牛の血液蛋白型支配遺伝子構成Oral presentation
- 第104回日本畜産学会大会. 要旨集97, 2005, Japanese, 不明, .東京., Domestic conferenceカンボジアにおける在来家畜及び近縁野生種の遺伝資源学的調査研究（概要）Oral presentation
- 第104回日本畜産学会大会. 要旨集96, 2005, Japanese, 不明, .東京., Domestic conferenceウシβグロビンにおける電気泳動的多型のDNAレベルでの解明Oral presentation
- 日本動物遺伝育種学会大会第6 回大会. 要旨集99, 2005, Japanese, 不明, 札幌, Domestic conferenceウシSREBP-1 遺伝子における変異と脂肪酸組成との関連Oral presentation
- 第104回日本畜産学会大会. 要旨集95, 2005, Japanese, 不明, 東京, Domestic conferenceSRY 遺伝子とミトコンドリアDNA の多型を指標にしたカンボジア在来牛における北方系牛とインド系牛の遺伝的寄与の評価.Oral presentation
- 第104回日本畜産学会大会. 要旨集96, 2005, Japanese, 不明, .東京., Domestic conferencemtDNAの解析に基づいたカンボジア在来ブタとカンボジア産イノシシの系統関係Oral presentation
- 第3回神戸大学農学部秋季公開セミナー －農学が築く食の安全と安心－, Nov. 2004, Japanese, 神戸大学農学部, 兵庫県私学会館, Domestic conference牛肉の素性を探る －品種鑑定により偽装表示を防ぐ－Oral presentation
- 第2回農学バイオフォーラム－バイオを利用したニュービジネス（講演会・パネル展示・相談会）－, Feb. 2004, Japanese, 神戸大学農学部, 神大会館（六甲ホール）, Domestic conferenceDNA診断による牛肉品質の向上への取り組みOral presentation
- 日本家禽学会2004年度秋季大会, 2004, Japanese, 日本家禽学会, 広島, Domestic conferenceカンボジアおよびその近隣東南アジア諸国におけるセキショクヤケイおよび在来鶏の分子系統学的解析Oral presentation
- 未記入, 2004, English, 未記入, 未記入, International conferenceSelective genotyping for marker assisted selection in Japanese Black cattle.Oral presentation
- 29th International Conference on Animal Genetics., 2004, English, 未記入, 未記入, International conferenceProteomic analysis using two-dimensional gel electrophoresis in muscle and fat tissue between Japanese Black and Holstein cattle.Oral presentation
- 29th International Conference on Animal Genetics, 2004, English, 未記入, 未記入, International conferenceMuscular dystrophy candidate gene in chicken genome.Oral presentation
- 29th International Conference on Animal Genetics., 2004, English, 未記入, 未記入, International conferenceMitochondrial DNA diversity in East Asian native goats.Oral presentation
- 29th International Conference on Animal Genetics., 2004, English, 未記入, 未記入, International conferenceExpression of Stearoyl-CoA Desaturase mRNA in bovine preadipocyte during differentiation.Oral presentation
- 29th International Conference on Animal Genetics., 2004, English, 未記入, 未記入, International conferenceConstruction of linkage map in Japanese quail (Coturnix Japonica) using AFLP, functional genes, and Chicken microsatellite marlkers.Oral presentation
- 29th International Conference on Animal Genetics. Pp.83, 2004, English, 未記入, 未記入, International conferenceConstruction of linkage map in Japanese quail (coturnix japonica) using AFLP, functional gene, and Chicken microsatellite markers.Oral presentation
- 29th International Conference on Animal Genetics., 2004, English, 未記入, 未記入, International conferenceComparisons between Japanese Black and Holstein cattle using gene expression profiling.Oral presentation
- 29th International Conference on Animal Genetics., 2004, English, 未記入, 未記入, International conferenceBreed Identification of beef cattle using SNPs markers derived from AFLP.Oral presentation
- 29th International Conference on Animal Genetics., 2004, English, 未記入, 未記入, International conferenceAllele frequencies of the extension locus encoding melanocortin-1 receptor (MC1R) in Japanese Brown(KOCHI variety) and Japanese Black.Oral presentation
- 日本動物遺伝育種学会大会第4回大会, Nov. 2003, Japanese, 日本動物遺伝育種学会, 明治大学生田校舎, Domestic conference黒毛和種繁殖雌牛集団におけるセレクティブ・ジェノタイピングOral presentation
- 日本動物遺伝育種学会大会第4回大会, Nov. 2003, Japanese, 日本動物遺伝育種学会, 明治大学生田校舎, Domestic conferenceニワトリCA3 遺伝子のクローニングと発現解析Oral presentation
- 日本動物遺伝育種学会大会第4回大会, Nov. 2003, Japanese, 日本動物遺伝育種学会, 明治大学生田校舎, Domestic conferenceウシ体脂肪の脂肪酸組成に影響する遺伝的要因Oral presentation
- 第53回関西畜産学会, Sep. 2003, Japanese, 関西畜産学会, 大田市民会館（島根）, Domestic conferenceエクソントラッピング法を用いたニワトリ機能遺伝子のマッピングOral presentation
- 在来家畜研究会, 日本動物遺伝育種学会合同公開シンポジウム, Mar. 2003, Japanese, 未記入, 未記入, Domestic conference山羊の起源と系譜Oral presentation
- 第101回日本畜産学会大会, Mar. 2003, Japanese, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, Domestic conference形態およびミトコンドリアDNA からみたミャンマーのスンクス野生集団のアジアにおける位置関係Oral presentation
- 第101回日本畜産学会大会, Mar. 2003, Japanese, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, Domestic conferenceミャンマー産野生イノシシおよび在来豚の核型解析Oral presentation
- 第101回日本畜産学会大会, Mar. 2003, Japanese, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, Domestic conferenceミャンマー在来鶏における在来鶏の卵白蛋白質多型Oral presentation
- 第101回日本畜産学会大会, Mar. 2003, Japanese, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, Domestic conferenceミャンマーの在来家畜に関する研究 ８ 在来鶏の血液蛋白質多型Oral presentation
- 第101回日本畜産学会大会, Mar. 2003, Japanese, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, Domestic conferenceミャンマーの在来家畜に関する研究 ７ ミャンマー在来アヒルのマイクロサテライト多型Oral presentation
- 第101回日本畜産学会大会, Mar. 2003, Japanese, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, Domestic conferenceミャンマーの在来家畜に関する研究 ６ ミャンマー馬の遺伝子構成Oral presentation
- 第101回日本畜産学会大会, Mar. 2003, Japanese, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, Domestic conferenceミャンマーの在来家畜に関する研究 ５ ミャンマー産在来羊の系統分類学的位置関係Oral presentation
- 第101回日本畜産学会大会, Mar. 2003, Japanese, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, Domestic conferenceミャンマーの在来家畜に関する研究 ３ 在来水牛の血液蛋白型支配遺伝子構成Oral presentation
- 第101回日本畜産学会大会, Mar. 2003, Japanese, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, Domestic conferenceミャンマーの在来家畜に関する研究 ２ 在来牛の血液蛋白型支配遺伝子構成Oral presentation
- 第101回日本畜産学会大会, Mar. 2003, Japanese, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, Domestic conferenceミャンマーの在来家畜に関する研究 1 ミャンマーにおける在来家畜の飼養現況Oral presentation
- 第101回日本畜産学会大会, Mar. 2003, Japanese, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, Domestic conferenceミタン(Bos gaurus)のプリオン遺伝子(PRNP)についてOral presentation
- 第101回日本畜産学会大会, Mar. 2003, Japanese, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, Domestic conferenceマイクロサテライト分析によるミャンマー在来ヤギの遺伝的多様性Oral presentation
- 第101回日本畜産学会大会, Mar. 2003, Japanese, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, Domestic conferenceウシ妊娠子宮内膜におけるelongation factor(EF)1A 遺伝子の発現Oral presentation
- 大学教育学会 （講演論文集pp.121-124), Mar. 2003, Japanese, 未記入, 神戸, Domestic conferencecDNAAFLP法を用いたウシ品種間における発現遺伝子の比較分析Oral presentation
- 第101回日本畜産学会大会, Mar. 2003, Japanese, 日本畜産学会, 畜産草地研究所（つくば国際会議場）, Domestic conferenceAFLP 法を用いたニホンウズラの連鎖地図作成.Oral presentation
- 畜産草地推進部会 問題別研究会, 2003, Japanese, 未記入, 未記入, Domestic conferenceミトコンドリアDNA 多型の産肉形質に及ぼす影響に関する研究の現状と今後の展開Oral presentation
■ Research Themes
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, Apr. 2024 - Mar. 2028Comprehensive Study of native Asian goats using whole genome information: New origins and migration routes of Southeast Asian Goats
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Kobe University, 07 Oct. 2021 - 31 Mar. 2026International joint research to elucidate the propagation of domestic animals across the Indian Ocean【研究の目的】本国際共同研究は「海の道」による家畜の海上伝播とその影響を明らかにすることを目的とし、着想したインド洋海上経路が中小家畜の伝播経路の一つであるという新しい仮説を検証する。その具体的方策として、インド洋海上経路の複数国（インドネシア、モーリシャス、マダガスカルなど）と国際共同研究を構築し、収集する中小家畜（ヤギ、ブタ、ニワトリ）試料に対し全ゲノムリシーケンスを含むゲノムワイドな遺伝学的解析を実施する。解析情報から広大なインド洋海路が家畜の伝播や遺伝子流入に与えた影響を評価し、ヒトの歴史に紐づけた家畜伝播の考察を行う。この解析には、家畜情報と試料（DNA）の収集という観点から、インド洋広範囲にわたる国際共同研究が必須となる。本課題により、インド洋海路に基づく新しい国際共同研究コンソーシアムを構築し、若手研究者参画によるグローバル視点を持つ将来の国際共同研究のリーダーを育成する。 【2021年度の研究実績】当該年度は世界的なコロナ禍が継続中のため、オンラインを使い国際共同研究者と調査計画を立案した。調査対象国はインドネシアとモーリシャスである。モーリシャスはFAREIのGobindram博士と連絡を取り、コロナ禍の状況を注視しながら2022年9月頃に2-3週間の調査を行う計画を立て、対象動物はヤギとニワトリである。モーリシャス本島とロドリゲス島の2島に対して調査を計画しつつある。インドネシアはHasanuddin大学のBugiwati 教授と連絡を取り、2022年度の調査計画を進めている。Lakor島、Timor島、Lembata島を対象とし、対象家畜はヤギである。コロナ禍の状況を鑑み、インドネシア共同研究者による試料収集を計画を進めている。採取された試料は冷凍保存し、機会を見て代表者が現地でのDNA精製等を行う予定としている。
- 日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2022 - 31 Mar. 2025ウシ脂肪交雑形成機構の解明に向けた候補遺伝子変異の網羅的検証
- 学術振興会, 科学研究費補助金／基盤研究(B), Apr. 2021 - Mar. 2024, Principal investigator東アジア牛は野生牛から遺伝子流入を受けたのか？－その遺伝構造と伝播経路の解明 ―"
- 科学研究費／挑戦的萌芽研究, Apr. 2019 - Mar. 2022, Principal investigator全ゲノム情報を用いたヤギの起源と伝播、環境適応形質の包括的解明に向けた先駆的研究Competitive research funding
- 学術振興会 研究動向調査費, Apr. 2018 - Mar. 2022, Principal investigator動物生産科学関連分野に関する学術研究動向―動物生産科学における新たな潮流―Competitive research funding
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 01 Apr. 2018 - 31 Mar. 2021Detection of candidate polymorphisms for beef marbling based on whole-genome resequencing dataWe performed genome-wide association study (GWAS) to identify the genomic region associated with beef marbling, and detected a candidate in BTA7 at 10-30 Mbp. Next, we comprehensively detected all polymorphisms in the candidate region using whole-genome resequencing data. Based on whole-genome resequencing of eight animals, we detected 127,090 polymorphisms within the region. Of these, 31,945 were located within the genes. We further narrowed the polymorphisms to 6,044 of 179 genes based on allele differences between the high and low marbling groups. We subsequently investigated the functions of these genes and selected 170 polymorphisms in eight genes as possible candidate polymorphisms. We focused on SLC27A6 K81M as a putative candidate polymorphism. We genotyped the SNP in a Japanese Black population (n = 904) to investigate the effect on marbling. Analysis of variance revealed that SLC27A6 K81M had a lower p-value (p = 0.0009) than the most significant SNP in GWAS (p = 0.0049).
- 科学研究費補助金 基盤研究B(海外学術調査), Apr. 2017 - Mar. 2020中央アジアにおけるウシ科，ラクダ科家畜とその近縁種の遺伝資源学的調査研究Competitive research funding
- 科学研究費補助金／基盤研究(B), Apr. 2017 - Mar. 2020, Principal investigatorCompetitive research funding
- 科学研究費補助金／基盤研究(B), Apr. 2016 - Mar. 2020, Principal investigatorCompetitive research funding
- 学術研究助成基金助成金／挑戦的萌芽研究, Apr. 2016 - Mar. 2018, Principal investigatorCompetitive research funding
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Hiroshima University, 01 Apr. 2014 - 31 Mar. 2017Molecular phylogenetic study on domestic process and global gene migration of chicken and pigsMolecular phylogenetic study on domestic process and global gene migration of chickens and pigs were carried out using mitochondrial DNA and single nucleotide polymorphisms as molecular markers. In phylogenetic tree of wild boars and pigs using SNPs, (1) separated clades were formed for each breed, subspecies or population, (2) Japanese wild boar (JWB) and Ryukyu one (RWB) separated into two clades obviously, and especially RWB had formed five clades in each island. In molecular analysis of chickens using mitochondrial DNA, it suggested the dual origins of African native chickens. The first group was probably originated from South Asia, and then migrated to West Asia, and finally arrived to Africa thorough Egypt. The second group migrated from Pacific to East Africa via Indian Ocean probably by Austronesian people. Therefore, these obtained findings are important for the better understanding of the diversity and uniqueness of chickens (junglefowls) and pigs (wild boars).
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Okayama University, 01 Apr. 2013 - 31 Mar. 2017Research on genetic diversity of native horses in mountainy ranges of South AsiaIn the present study we investigate genetic diversity and relation of Asian native horse populations using DNA samples collected from mountainy ranges Myanmar, Laos, and Vietnam. We collected 184, 71, and 167 DNA samples of native horses in Myanmar, Laos, and Vietnam, respectively, and investigated haplotypes of mitochondrial DNA and Y chromosomal markers as well as genotypes of microsatellite markers and several functional genes. The results indicated that genetic diversities of maternal lineages are high in these populations and the have unique genetic compositions, while a part of these populations showed genetic influences of western breeds in paternal lineage.
- 学術研究助成基金助成金／挑戦的萌芽研究, Apr. 2014 - Mar. 2016, Principal investigatorCompetitive research funding
- 科学研究費補助金 基盤研究B(海外学術調査), Apr. 2013 - Mar. 2016カザフスタンの在来家畜とその近縁野生種の遺伝資源学的研究および放射線影響評価Competitive research funding
- 革新的技術開発・緊急展開事業, 2016, Principal investigator国産和牛肉の新たな差別化のための評価指標及び育種手法の開発Competitive research funding
- ＤＮＡマーカー育種の高度化のための技術開発委託事業, 2016, Principal investigatorウシの繁殖性に関連するゲノム領域の探索とDNAマーカーの開発Competitive research funding
- 科学研究費補助金／基盤研究(C), Apr. 2012 - Mar. 2015Competitive research funding
- 科学研究費補助金／基盤研究(B), Apr. 2011 - Mar. 2014, Principal investigatorCompetitive research funding
- 科学研究費補助金 基盤研究B, Apr. 2010 - Mar. 2013新彊ウイグル自治区小河墓遺跡の学際的調査による砂漠化過程の解明Competitive research funding
- 科学研究費補助金 基盤研究C, Apr. 2009 - Mar. 2011変異型ユビキチンリガーゼを発現する筋ジストロフィーモデルマウスの開発と病態の解析Competitive research funding
- 科学研究費補助金 基盤研究B(海外学術調査), Apr. 2008 - Mar. 2009バングラデシュにおける在来家畜及び野生原種の遺伝資源学的・文化人類学的研究Competitive research funding
- 科学研究費補助金／基盤研究(C), Apr. 2007 - Mar. 2009, Principal investigatorCompetitive research funding
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Nagoya University, 2004 - 2007Preservation and utilization of genetic resources of domestic ruminants, based on studies of the origin and phylogenyWe have collected several thousands of DNA samples of ruminants, such as cattle, water buffaloes, sheep, goats, pigs, etc, in Japan and Asian countries through overseas scientific surveys and present research project Those invaluable DNA samples were data-filed by the present investigators in order to facilitate them to be supplied far researchers elsewhere. On the other hand, we analyzed those samples to get knowledge of the genetic-variability and -differentiation between local populations, and genotypic variations of several functional genes. 1.Research on genetic differentiation between populations (1) Domestic pigs and wild boars from respective areas of South and Southeast Asian continent were analyzed of the r mtDNA haplotypes, The result showed that some pigs carried identical mtDNA haplotypes to those of wild boars inhabiting same area, i.e. polyphyletic origin of the pig. 2.Researches on genotypic variations of ruminant functional genes (1) Expression of a cluster of genes related to regulates of seasonal breeding of animals was examined by methods of system biology, we found that the TSH. elevation in MBH increased DIO2 gene expression followed by other gene expressions, indicating TSH in MBH is a trigger for the photoperiodic activities. (2) Polymorphism in B2C high-sulfur wool protein alleles was examined in sheep from China and East Asia and compared to European breeds. The local breeds of northern part of the area showed a similar allelic compositions to European breeds, but the southern local populations were marked different from them in the allelic frequencies. (3)Purion protein genotypes were examined in a variety of local or native populations, and found the scrapie sensitive genotype ARQ/ARQ is highly prevalent in East Asian local breeds or native populations.
- 科学研究費補助金 若手研究B, Apr. 2003 - Mar. 2005, Principal investigatorニホンウズラにおける遺伝連鎖地図と比較染色体地図の作製Competitive research funding
- 科学研究費補助金 基盤研究B, Apr. 2002 - Mar. 2004, Principal investigatorAFLP法によるウシ・ゲノム解析とその応用Competitive research funding
- 科学研究費補助金 基盤研究B(海外学術調査), Apr. 2001 - Mar. 2003ミャンマーにおける在来家畜と近縁野生種の遺伝資源学的調査と保全施策に関する研究Competitive research funding
- 日本学術振興会, 科学研究費助成事業, 萌芽研究, 神戸大学, 2002 - 2003黒毛和種遺伝子発現のDNAマイクロアレーによる解析家畜のゲノム研究は急速に発展し、ウシ全ゲノム配列が決定されようとしている。この段階に来て、家畜の経済的な形質に関連する全遺伝子を把握し、家畜の選抜や効率の良い生産を目指す学問が台頭してきた。それが、バイオインフォマティックスである。このバイオインフォマティックス研究にはマイクロアレーを利用した、網羅的・総合的な遺伝子解析が必要となる。我々は、オーストラリアのCooperative Research Center for Cattle and Beef Quality (CRCMQ)、CSIROと共同で、牛肉の肉質の改善に係わる遺伝子を発見するためのcDNAマイクロアレーの製作と、それを用いた分析に取り組んできた。実際には裾、神戸大学農学部附属農場にて、黒毛和種、ホルスタイン種、両者の一代雑種、夫々3頭を供試し、経時的にロース部分の筋肉(約1g)を生体より採取し、mRNAの分析、アミノ酸組成の分析、脂肪酸組成の分析等に取り組んだ。 mRNAの分析ではこれらの経時的に採取したサンプルと屠殺時に採取したサンプルとを9、000種類の遺伝子を貼り付けたcDNAマイクロアレーによって分析した。マイクロアレーの作成と分析はオーストラリア側が行った。その結果、肥育開始直後の10ヶ月齢付近で、品種間で著しく異なる遺伝子が、多数発見された。そのうちの一つは、以前から見当をつけていた遺伝子、Stearoyl coAdesaturase (SCD)で、この遺伝子が、牛肉の品質の指標になる事が改めて確認された。また、その他多数の遺伝子が発見されているので、今後はSCD以外の個々の遺伝子一つずつについて、Real-time PCR法などによって経時的に分析に、複数の遺伝子の経時的な動態が黒毛和種の肉質の改善にどのように係わっているかを明らかにしてゆく。この研究によって、何時の時期にどの遺伝子を指標にして調べれば、屠殺時の肉質の指標となるか、肉質改善の目標として、どのような遺伝子を対象にすれば肉質の改善が図れるかが明らかになろうとしていて、極めて期待の大きい成果が得られている。次いで、8頭の黒毛和種を供試し、ビタミンA濃度の肉質に関する影響を分析中で、ビタミンAの肉質に関わる遺伝子に及ぼす影響の発現解析が可能になる。
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Nagoya University, 2002 - 2003Survey of genetic resources on native domestic animals and the wild relatives in Cambodia1. In Preliminary Survey (H.14.8.18-25), First Survey (H14.12.19-H15.1.16) and Third Survey (H15.12.18-H16.1.14) in Cambodia, we got genetical, morphological or other data of domestic and wild type of animals by field observation, investigation on farming materials, measurements of animals or analyses of biological materials in 18 provinces of the country. Those data were compiled to subject further comparative analyses with those of other animals in other Asian countries we investigated so far. We still continue detailed experimental analyses with biological materials. Studies on history of establishment of Asian native domestic animals, genetic-resource evaluation and its cultural aspects will be extended. 2. Data or preliminary results obtained are as follows : (1) Cattle:field observation of coat-color variation of 28140 head, body-measurements of more than 200 head, blood samples of 212 head which were subjected to electrophoretic analyses for 21 genetic loci and DNA extraction, and collection of several cultural materials for museum exhibition. (2) Similarly in many individuals of water buffaloes, goats, pigs, horses, chickens and ducks, morphological or biological materials and data could be obtained. (3) biological materials of wild animals such as jungle fowls, wild boars and house shrews were also obtained. After detailed experimental analyses of those materials, most of research results concerned with Cambodian domestic and the wild relatives will be opened in the special issue of Report of the Society for Researches on Native Livestock (No.22) published in 2006.
- 科学研究費補助金 奨励研究A, Apr. 2000 - Mar. 2002, Principal investigatorAFLP法を用いたニホンウズラの連鎖地図構築Competitive research funding
- 科学研究費補助金 奨励研究A, Apr. 1999 - Mar. 2001, Principal investigatorAFLP法を用いたニワトリ筋ジストロフィー遺伝子の連鎖分析Competitive research funding
- 委任経理金・伊藤記念財団, Apr. 1999 - Mar. 2000, Principal investigatorミトコンドリアDNAを用いた我国の黒毛和種とホルスタイン種に対する比較遺伝構造分析Competitive research funding
- 委任経理金・伊藤記念財団, Apr. 1998 - Mar. 1999, Principal investigator毛根を用いた黒毛和種におけるミトコンドリアD-loopのSSCP分析Competitive research funding
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 1998 - 1999Polymorphism's of mitochondrial DNA and selection of Japanese BlackJapanese black is only one domestic animal which produce economically competitive beef with foreign products, because of producing high grade beef. The genes by which the high quality beef being produced are not yet known. Almost of all researches are concentrated their study on the genome DNA from the nuclei, however, we had paid attention on the genome from mitochondria for past few years. Japanese Black are classified into five groups depending on D-loop DNA sequence of mitochondria. We found that a group, type 4, produce high quality beef. 1)To ascertain if this mitochondrial effect is observed in commercial reproductive herd, cows of more than 8,000 were classified into 5 groups by mitochondrial DNA type. The effect of mitochondrial type is not clear because of environmental effects, such as feeding condition, year season, feedlot etc. But in Hyogo prefecture, the mitochondrial effect was only observed in steers of 28month old finishing. If D-loop has some physiological effects on beef production, as the D-loop region does not code any protein, so that mutations other region than D-loop region, which coordinates with D-loop type should be present. We selected two animals from each mitochondrial type and DNA sequence of whole mitochondria genome was determined. We found type 4 specific mutation in 16SrRNA which may affect on protein synthesis in mitochondria.
- 科学研究費補助金 国際学術研究, Apr. 1997 - Mar. 1998ラオスにおける動物資源の遺伝学的調査Competitive research funding
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), KOBE UNIVERSITY, 1994 - 1996GENETIC IMPROVEMENT OF JAPANESE BLACK CATTLE BY USING SINGLE LOCUS VNTR MARKERS.Toward efficient improvements of Japanese Black cattle, we made a plan to introduce marker assisted selection procedures. DNA markers are useful tools for the purpose. However, almost all DNA markers now available are dinucleotide repeats. The analysis of dinucleotide erpeats loci requires a special expensive apparatus and high running costs. Therefore, we are planned cloning of tri or tetra nucleotide repeats loci and/or more longer minisatellite loci. Using bovine genomic libray, we had picked up more than 20 clones of tetra nucleotide repeats loci using (GGAT) _4 as a probe. These clones contained (GGAT) _n sequence, but showed incomplete repeats less than 5 repeats, showing no length variation. For (GTG) repeat, we get one clone which is preferable for DNA markers ; heterozygosity of the loci is more than 87% and the polymorphism is easily detectable by using conventional agarose gel electrophoresis. On the other hand, cloning of microsatellite using VNTR 33.15 as a probe, we also obtained 20 positive clones. Employing Southern hybridization technology, we get a few clone of polymorphic.
- 家畜改良推進事業費補助金, Principal investigator家畜改良推進事業Competitive research funding
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