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KAWAGUCHI Fuki
Graduate School of Agricultural Science / Department of Bioresource Science
Assistant Professor

Researcher basic information

■ Research Keyword
  • Animal Breeding and Genetics
■ Research Areas
  • Life sciences / Animal production science
■ Committee History
  • Apr. 2022 - Present, 日本畜産学会, 若手企画委員

Research activity information

■ Award
  • Sep. 2022 日本畜産学会, 2022年度日本畜産学会奨励賞, 黒毛和種における筋肉内脂肪酸組成に関わる遺伝子多型の同定
    川口 芙岐

  • Mar. 2018 日本畜産学会第124回大会, 優秀発表賞, ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索
    川口芙岐, 木越洸利, 小浜菜美子, 秋山敬孝, 福島護之, 吉田恵実, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

  • Nov. 2016 日本動物遺伝育種学会第17回大会, 学会長特別賞, 黒毛和種繁殖雌牛集団におけるレプチン遺伝子内多型と繁殖形質との関連
    川口芙岐, 深澤謙治, 小浜菜美子, 秋山敬孝, 福島護之, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

■ Paper
  • Ryo Masuko, Ayin, Maho Masaoka, Fuki Kawaguchi, Shinji Sasazaki, Muhammad I. A. Dagong, Sri R. A. Bugiwati, Joseph S. Masangkay, Takahiro Yonezawa, Hideyuki Mannen
    Springer Science and Business Media LLC, Mar. 2025, Scientific Reports, 15(1) (1)
    [Refereed]
    Scientific journal

  • Ayin, Ryo Masuko, Naho Kakutani, Fuki Kawaguchi, Shinji Sasazaki, Sanjar Sultankulov, Asankadyr Zhunushov, Masahide Nishibori, Takahiro Yonezawa, Hideyuki Mannen
    Sep. 2024, Animal Science Journal, 95, e13999, English, International magazine, Co-authored internationally
    [Refereed]
    Scientific journal

  • Shinji Sasazaki, Hina Kondo, Yurika Moriishi, Fuki Kawaguchi, Kenji Oyama, Hideyuki Mannen
    Abstract Background Beef marbling is considered a desirable trait in the meat industry. Therefore, understanding the genetic factors that cause marbling is important. Previously, we performed a genome-wide association study to examine genetic factors associated with beef marbling in Japanese Black cattle and identified a candidate region between 10–30 Mbp on chromosome 7. We verified the effect of the SNPs in this region on beef marbling using linkage disequilibrium block analysis. We narrowed down the candidate region to a range of 15.8–16.1 Mbp. In this study, we comprehensively detected all of the SNPs in this region and verified their effects on beef marbling. Results Genome resequencing using four animals exhibiting high beef marbling standard (BMS) and four with low BMS revealed a total of 1,846 polymorphisms within the candidate region. Based on the annotation, we selected 13 SNPs exhibiting a moderate impact, as no high-impact SNPs were detected. All of the SNPs represented missense polymorphisms and were located in the following seven genes: RDH8, ANGPTL6, DNMT1, MRPL4, ICAM1, ICAM3, and ICAM5. Finally, we determined the effects of these SNPs on the BMS of a Japanese Black cattle population (n = 529). Analysis of variance revealed that the five SNPs were located in genes encoding the intercellular adhesion molecules (ICAM1, ICAM3, and ICAM5), and showed a highly significant association compared with the remainder (p < 0.01). The lowest p-value was observed for ICAM3_c.739G > A (p = 1.18E-04). Previous studies have suggested that intercellular adhesion molecules (ICAM) may be an upstream factor that regulates adipocyte differentiation. Therefore, considering the polymorphism and putative gene function, we suggest that ICAM1 is potentially responsible for beef marbling. c.470C > G and/or c.994G > A on ICAM1 may be responsible for this quantitative trait locus. Conclusions Promising SNP candidates responsible for beef marbling were identified using extensive polymorphism verification in a previously reported QTL region. We aim to elucidate the mechanism of beef marbling in future studies by investigating how these polymorphisms alter protein structure and function.
    Springer Science and Business Media LLC, Feb. 2024, BMC Genomic Data, 25(1) (1), English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • 黒毛和種の筋肉内脂肪における脂肪酸組成関連遺伝子多型の同定
    川口芙岐
    May 2023, 日本畜産学会報, 94(2) (2), 133 - 141, Japanese
    [Refereed]

  • Isaäc J. Nijman, Benjamin D. Rosen, Philippe Bardou, Thomas Faraut, Tristan Cumer, Kevin G. Daly, Zhuqing Zheng, Yudong Cai, Hojjat Asadollahpour, Bengi Çınar Kul, Wei‐Yi Zhang, Guangxin E., A. Ayin, Hayley Baird, Meirat Bakhtin, Valentin A. Bâlteanu, Diana Barfield, Beate Berger, Thor Blichfeldt, Geert Boink, Sri R. A. Bugiwati, Zexi Cai, Sean Carolan, Emily Clark, Vlatka Cubric‐Curik, Muhammad I. A. Dagong, Tashi Dorji, Louise Drew, Jiazhong Guo, Jón Hallsson, Simon Horvat, Juha Kantanen, Fuki Kawaguchi, Polat Kazymbet, Negar Khayatzadeh, Namshin Kim, Manoj Kumar Shah, Yuying Liao, Amparo Martínez, Joseph S. Masangkay, Maho Masaoka, Raffaele Mazza, John McEwan, Marco Milanesi, Faruque Md. Omar, Yuto Nomura, Nadjet‐Amina Ouchene‐Khelifi, Filipe Pereira, Goutam Sahana, Mazdak Salavati, Shinji Sasazaki, Anne Da Silva, Mojca Simčič, Johann Sölkner, Alison Sutherland, Johannes Tigchelaar, Hongpin Zhang, Paolo Ajmone‐Marsan, Daniel G. Bradley, Licia Colli, Cord Drögemüller, Yu Jiang, Chuzhao Lei, Hideyuki Mannen, François Pompanon, Gwenola Tosser‐Klopp, Johannes A. Lenstra, James Kijas, Bernt Guldbrandtsen, Laure Denoyelle, Julien Sarry, Estel le Talouarn, Adriana Alberti, Céline Orvain, Stefan Engelen, Dylan Duby, Pierre Martin, Coralie Danchin, Delphine Duclos, Daniel Allain, Remy Arquet, Nathalie Mandonnet, Michel Naves, Isabelle Palhiere, Rachel Rupp, Francois Pompanon, Hamid R. Rezaei, Maeve Foran, Alessandra Stella, Marcello Del Corvo, Alessandra Crisa, Donata Marletta, Paola Crepaldi, Michele Ottino, Ettore Randi, Denis Fidalis Mujibi, Timothy Gondwe, Badr Benjelloun, Maria Da Gloria Taela, Oyekan Nash, Muhammad Moaeen‐ud‐Din, Carina Visser, Felix Goyache, Isabel Alvarez, Marcel Amills, Armand Sànchez, Juan Capote, Jordi Jordana, Agueda Pons, Illes Balears, Antonio Molina, Hassan Ally Mruttu, Clet Wandui Masiga, Curtis P. Van Tassell, Jim Reecy, Gordon Luikart, Joseph Sikosana, Hoda Anila, Dobi Petrit, Baumung Roswitha, Baret Philippe, Fadlaoui Aziz, Papachristoforou Christos, M. A. A. El‐Barody, Taberlet Pierre, England Phillip, Luikart Gordon, Beja‐Pereira Albano, Zundel Stéphanie, Trommetter Michel, Erhardt Georg, Brandt Horst, Ibeagha‐Awemu Eveline, Gesine Lühken, Daniela Krugmann, Prinzenberg Eva‐Maria, Lipsky Shirin, Gutscher Katja, Peter Christina, Roosen Jutta, Bertaglia Marco, Georgoudis Andreas, Jamil Al Tarrayrah, Kliambas Georgios, Kutita Olga, Karetsou Katerina, Ligda Christina, Istvan Anton, Fesus Lazlo, Canali Gabriele, Milanesi Elisabetta, Pellecchia Marco, Carta Antonello, Sechi Tiziana, Cicogna Mario, Fornarelli Francesca, Giovenzana Stefano, Marilli Marta, S. Bordonaro, D’ Urso Giuseppe, Pilla Fabio, D’ Andrea Mariasilvia, Valentini Alessio, Cappuccio Irene, Pariset Lorraine, Abo‐Shehada Mahamoud, Lisette M. Van Cann, Niznikowski Roman, Dominik Popielarczyk, Strzelec Ewa, Vlaic Augustin, Dunner Susana, Canon Javier, Cortes Oscar, Garcia David, Caloz Régis, Obexer‐Ruff Gabriela, Marie‐Louise Glowatzki, Ertugrul Okan, Togan Inci, Koban Evren, Bruford Mike, Perez Trinidad, Juma Gabriela, Hewitt Godfrey, Dalamitra Stella, Wiskin Louise, Taylor Martin, Jones Sam, Scarpa Riccardo
    Wiley, Aug. 2022, Molecular Ecology, 31(16) (16), 4364 - 4380, English
    [Refereed]
    Scientific journal

  • Shinji Sasazaki, Raito Yamamoto, Shintaro Toyomoto, Hina Kondo, Takayuki Akiyama, Namiko Kohama, Emi Yoshida, Fuki Kawaguchi, Kenji Oyama, Hideyuki Mannen
    In our previous study, we used genome resequencing to detect all candidate polymorphisms within a quantitative trait loci (QTL) region for beef marbling reported previously at 10–30 Mbp on bovine chromosome 7, and we selected 6044 polymorphisms as candidate quantitative trait nucleotides (QTNs). In the present study, we aimed to identify quantitative trait genes (QTGs) and QTNs in this QTL region by verifying the effect of SNPs on beef marbling in two Japanese Black cattle populations using a Dynamic Array integrated fluidic circuit. In total, 96 selected SNPs were genotyped in 441 and 529 animals in Hyogo and Miyazaki cattle populations, respectively. The most significant p-values were detected in a SNP in a splice region of ALDH7A1 (SNP93_ALDH7A1; p = 3.46 × 10−5) in Hyogo cattle and a missense polymorphism of intercellular adhesion molecule-1 (ICAM1) (SNP37_ICAM1; p = 3.33 × 10−4) in Miyazaki cattle. Interestingly, SNP93_ALDH7A1 was not significant (p = 0.459) in Miyazaki cattle, and SNP37_ICAM1 showed a weakly significant association (p = 0.043) in Hyogo cattle. Thus, each population would likely have different QTGs and QTNs for beef marbling in the QTL region. In the Hyogo population, it was not possible to determine the accurate range of the linkage disequilibrium (LD) block in LD block analysis because of a strong LD structure throughout the assessed region. In Miyazaki cattle, however, an LD block containing SNP37_ICAM1 had a range of 15.8–16.1 Mbp, suggesting that QTNs would be located within this region. The functions of 19 genes in the LD block were investigated. ICAM1 is known to play an important role in adipocyte differentiation; given this function and the effect of amino acid substitution, SNP37_ICAM1 was identified as a promising candidate QTN for beef marbling. Further research on the effect of SNP37_ICAM1 on adipocyte differentiation is expected to provide insights into the mechanism underlying beef marbling formation.
    MDPI AG, Jul. 2022, Genes, 13(7) (7), 1190 - 1190, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Kohei Nishikaku, Takahiro Yonezawa, Masahide Nishibori, Masashi Harada, Fuki Kawaguchi, Shinji Sasazaki, Yasushi Torii, Kazuhiko Imakawa, Kuniko Kawai, Jianquan Liu, Hideyuki Mannen, Tomoko Kobayashi
    Bovine leukemia virus (BLV), the causative agent of enzootic bovine leukosis, is currently one of the most important pathogens affecting the cattle industry worldwide. Determining where and in which host it originated, and how it dispersed across continents will provide valuable insights into its historical emergence as the cattle pathogen. Various species in the Bos genus were domesticated in Asia, where they also diversified. As native cattle (taurine cattle, zebu cattle, yak, and water buffalo) are indigenous and adapted to local environments, we hypothesized that Asian native cattle could have harbored BLV and, therefore, that they were important for virus emergence, maintenance, and spread. In this study, phylogeographic and ancestral trait analyses—including sequences obtained from Asian native cattle—were used to reconstruct the evolutionary history of BLV. It was shown that, since its probable emergence in Asia, the virus spread to South America and Europe via international trade of live cattle. It was inferred that zebu cattle were the hosts for the early origin of BLV, while taurine cattle played the significant role in the transmission worldwide. In addition, the results of positive selection analysis indicate that yak had a substantially minor role in the transmission of this virus. In this study, endogenous deltaretrovirus sequences in bats, collected in Asian countries, were also analyzed on whether these sequences were present in the bat genome. Endogenous deltaretrovirus sequences were detected from bat species endemic to specific regions and geographically isolated for a long time. Endogenous deltaretrovirus sequences from these geographically isolated species represent ancient exogenous deltaretroviruses distributions. The phylogenetic analysis revealed that these newly obtained endogenous deltaretrovirus sequences were closely related to those of BLV from Asian native cattle, indicating that BLV-related ancient deltaretroviruses circulated in Asia long before the emergence of BLV. Together, our analyses provide evidence for origin and spatiotemporal dynamics of BLV.
    Frontiers Media SA, Jun. 2022, Frontiers in microbiology, 13, 917324 - 917324, English, International magazine
    [Refereed]
    Scientific journal

  • Fuki Kawaguchi, Mitsuki Nakamura, Eiji Kobayashi, Takahiro Yonezawa, Shinji Sasazaki, Hideyuki Mannen
    Lead, Wiley, Jan. 2022, Animal Science Journal, 93(1) (1), e13770, English
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Fuki Kawaguchi, Fuka Kakiuchi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki
    Five polymorphisms associated with the percentage of oleic acid (C18:1) in beef fat were previously reported on bovine chromosome 19 in different Japanese Black cattle populations. This study aimed to verify the effects of these five polymorphisms on C18:1 using the same Japanese Black cattle population and conduct linkage disequilibrium (LD) analysis in order to determine the locations of the quantitative trait loci (QTLs). We genotyped the five polymorphisms (SREBP1 c.1065 + 83 (84bp indel), STARD3 c.1187 C > T, GH c.379 C > G, FASN g.841 G > C, and FASN g.16024 A > G) in two populations, which were bred in Hyogo and Gifu Prefectures, Japan (n = 441 and 443, respectively) in order to analyze their effects on C18:1 using analysis of variance (ANOVA). In the Hyogo population, SREBP1 c.1065 + 83 and STARD3 c.1187 C > T were significantly associated with C18:1 (p < 0.001). Meanwhile, FASN g.841 G > C, FASN g.16024 A > G, and GH c.379 C > G were significantly associated with C18:1 (p < 0.01) in the Gifu population. LD analysis was subsequently conducted to detect the range of the QTLs, which ranged from 32.2 to 46.4 Mbp and from 47.8 to 52.1 Mbp in the Hyogo and Gifu populations, respectively. In conclusion, this study confirmed the existence of QTLs on BTA19 and divided the candidate region for each QTL based on LD coefficients. These results could contribute to efficient searches for responsible genes and polymorphisms for fatty acid composition.
    Lead, MDPI AG, Jun. 2021, Life, 11(7) (7), 597 - 597
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Shinji Sasazaki, Koichiro Tomita, Yuto Nomura, Fuki Kawaguchi, Tetsuo Kunieda, Manoj Kumar Shah, Hideyuki Mannen
    Wiley, Jan. 2021, Animal Science Journal, 92(1) (1), e13640, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Recent achievements of candidate polymorphism detection for fatty acid composition in Japanese Black cattle
    Fuki KAWAGUCHI, Hideyuki MANNEN, Shinji SASAZAKI
    Lead, 2021, The Journal of Animal Genetics, 49, 67 - 75, English
    [Refereed]

  • Hideyuki Mannen, Takahiro Yonezawa, Kako Murata, Aoi Noda, Fuki Kawaguchi, Shinji Sasazaki, Anna Olivieri, Alessandro Achilli, Antonio Torroni
    AbstractSurveys of mitochondrial DNA (mtDNA) variation have shown that worldwide domestic cattle are characterized by just a few major haplogroups. Two, T and I, are common and characterize Bos taurus and Bos indicus, respectively, while the other three, P, Q and R, are rare and are found only in taurine breeds. Haplogroup P is typical of extinct European aurochs, while intriguingly modern P mtDNAs have only been found in northeast Asian cattle. These Asian P mtDNAs are extremely rare with the exception of the Japanese Shorthorn breed, where they reach a frequency of 45.9%. To shed light on the origin of this haplogroup in northeast Asian cattle, we completely sequenced 14 Japanese Shorthorn mitogenomes belonging to haplogroup P. Phylogenetic and Bayesian analyses revealed: (1) a post-glacial expansion of aurochs carrying haplogroup P from Europe to Asia; (2) that all Asian P mtDNAs belong to a single sub-haplogroup (P1a), so far never detected in either European or Asian aurochs remains, which was incorporated into domestic cattle of continental northeastern Asia possibly ~ 3700 years ago; and (3) that haplogroup P1a mtDNAs found in the Japanese Shorthorn breed probably reached Japan about 650 years ago from Mongolia/Russia, in agreement with historical evidence.
    Springer Science and Business Media LLC, Nov. 2020, Scientific Reports, 10(1) (1), 20842
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Indonesian native goats (Capra hircus) reveal highest genetic frequency of mitochondrial DNA haplogroup B in the world
    Hideyuki Mannen, Kenta Iso, Fuki Kawaguchi, Shinji Sasazaki, Takahiro Yonezawa, Muhammad Ihsan, Andi Dagong, Sri Rachma Aprilita Bugiwati
    Sep. 2020, Animal Science Journal, 91(1) (1), e13485
    [Refereed]
    Link to Kobe Univ. Repository (Kernel)

  • Fuki Kawaguchi, Miyako Tsuchimura, Kenji Oyama, Tamako Matsuhashi, Shin Maruyama, Hideyuki Mannen, Shinji Sasazaki
    Abstract. Carcass traits have been efficiently improved by recent selection using DNA markers in beef cattle. Additionally, DNA markers might have an effect on other traits such as fertility traits; therefore attention should also be paid to such pleiotropic effects. However, the effects of the markers on both carcass and fertility traits have never been evaluated in the same population, since they are generally measured in different populations. The objective in the current study was to discuss effectiveness of DNA markers developed for carcass traits through investigation of their effects on carcass and fertility traits in a population. We genotyped six markers SCD V293A, FASN g.841G>C, PLAG1 g.49066C>G, NCAPG I442M, DGAT1 K232A, and EDG1 g.1471620G>T in a Japanese Black cattle population (n=515). To investigate their effects on carcass and fertility traits, we performed statistical analysis (ANOVA and the Tukey–Kramer honestly significant difference (HSD) test). In the results, three of six markers, SCD V293A, NCAPG I442M, and EGD1 g.1471620G>T, were significantly associated with both carcass and fertility traits. Remarkably, the same allele for each marker had positive effects on both traits, suggesting that we would be able to simultaneously improve them using these markers in this population. However, previous studies reported that the effects of DNA markers could differ among populations. Therefore, it is necessary to confirm the effect of the marker in each population before it is used for improvement.
    Lead, Copernicus GmbH, Jan. 2020, Archives Animal Breeding, 63(1) (1), 9 - 17, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Shinji Sasazaki, Fuki Kawaguchi, Ayaka Nakajima, Raito Yamamoto, Takayuki Akiyama, Namiko Kohama, Emi Yoshida, Eiji Kobayashi, Takeshi Honda, Kenji Oyama, Hideyuki Mannen
    In our previous study, we performed genome-wide association study (GWAS) to identify the genomic region associated with Fat area ratio to rib eye area (FAR) and detected a candidate in BTA7 at 10-30 Mbp. The present study aims to comprehensively detect all polymorphisms in the candidate region using whole-genome resequencing data. Based on whole-genome resequencing of eight animals, we detected 127,090 polymorphisms within the region. Of these, 31,945 were located within the genes. We further narrowed the polymorphisms to 6,044 with more than five allele differences between the high and low FAR groups that were located within 179 genes. We subsequently investigated the functions of these genes and selected 170 polymorphisms in eight genes as possible candidate polymorphisms. We focused on SLC27A6 K81M as a putative candidate polymorphism. We genotyped the SNP in a Japanese Black population (n = 904) to investigate the effect on FAR. Analysis of variance revealed that SLC27A6 K81M had a lower p-value (p =.0009) than the most significant SNP in GWAS (p =.0049). Although only SLC27A6 K81M was verified in the present study, subsequent verification of the remaining candidate genes and polymorphisms could lead to the identification of genes and polymorphisms responsible for FAR.
    WILEY, Jan. 2020, ANIMAL SCIENCE JOURNAL, 91(1) (1), English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Risa Tabata, Fuki Kawaguchi, Shinji Sasazaki, Yoshio Yamamoto, Felix Rakotondraparany, Fanomezana Mihaja Ratsoavina, Takahiro Yonezawa, Hideyuki Mannen
    Zoological Society of Japan, Aug. 2019, Zoological Science, 36(4) (4), 294 - 294, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Hirokazu Matsumoto, Fuki Kawaguchi, Shotaro Itoh, Suzuna Yotsu, Kento Fukuda, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki
    Elsevier BV, Jun. 2019, Meta Gene, 20, 100558 - 100558, English
    [Refereed]
    Scientific journal

  • Fuki Kawaguchi, Hiroto Kigoshi, Moriyuki Fukushima, Eiji Iwamoto, Eiji Kobayashi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki
    Lead, Wiley, Apr. 2019, Animal Science Journal, 90(4) (4), 467 - 472, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Risa Tabata, Fuki Kawaguchi, Shinji Sasazaki, Yoshio Yamamoto, Meirat Bakhtin, Polat Kazymbet, Alykhan Meldevekob, Maratbek Z Suleimenov, Masahide Nishibori, Hideyuki Mannen
    Goats (Capra hircus) were domesticated in the Fertile Crescent and propagated all over the world. The Silk Road through the Eurasian Steppe belt is a possible propagation route for domestic goats to Central Asia. Kazakhstan is in close geographical proximity to domestication centers and covers the majority of the Eurasian Steppe belt. In this study, we examined the genetic diversity and phylogeographic structure of Kazakhstani goats. The mtDNA sequences of 141 Kazakhstani goats were categorized into haplogroups A, C, and D, of which haplogroup A was predominant (97%), whereas haplogroups C and D were detected at low frequencies (1.4% each). The Kazakhstani haplotypes C were thzen categorized into Asian mtDNA type. Sequence analysis of the SRY gene on the Y-chromosome in 67 male Kazakhstani goats revealed two haplotypes: Y1A (64%) and Y2A (36%). Analysis of the distribution of mtDNA haplogroups and SRY haplotypes from Eurasia and Africa demonstrated genetic similarity among animals from Kazakhstan, Mongolia, and Northwest China located on the Eurasian Steppe belt. These phylogeographic results suggested that the Eurasian Steppe belt was an important propagation route for goats to Central Asia.
    Wiley, Mar. 2019, Animal science journal = Nihon chikusan Gakkaiho, 90(3) (3), 317 - 322, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Hiroto Kigoshi, Fuki Kawaguchi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki
    Japanese Society of Animal Breeding and Genetics, 2019, The Journal of Animal Genetics, 47(2) (2), 37 - 45, English
    [Refereed]
    Scientific journal

  • Hayate Yamanaka, Kako Murata, Risa Tabata, Fuki Kawaguchi, Shinji Sasazaki, Yoshio Yamamoto, Meirat Bakhtin, Polat Kazymbet, Alykhan Meldevekob, Maratbek Z Suleimenov, Masahide Nishibori, Hideyuki Mannen
    Kazakhstan is the largest landlocked country and contains two important propagation routes for livestock from the Fertile Crescent to Asia. Therefore, genetic information about Kazakhstani cattle can be important for understanding the propagation history and the genetic admixture in Central Asian cattle. In the present study, we analyzed the complete mtDNA D-loop sequence and SRY gene polymorphism in 122 Kazakhstani native cattle. The D-loop sequences revealed 79 mitochondrial haplotypes, with the major haplogroups T and I. The Bos taurus subhaplogroups consisted of T (3.3%), T1 (2.5%), T2 (2.5%), and T4 (0.8%) in addition to the predominant subhaplogroup T3 (86.9%), and the Bos indicus subhaplogroup of I1 (4.1%). Subsequently, we investigated the paternal lineages of Bos taurus and Bos indicus, however, all Kazakhstani cattle were shown to have Y chromosome of Bos taurus origin. While highly divergent mtDNA subhaplogroups in Kazakhstani cattle could be due to the geographical proximity of Kazakhstan with the domestication center of the Fertile Crescent, the absence of Bos indicus Y chromosomes could be explained by a decoupling of the introgression dynamics of maternal and paternal lineages. This genetic information would contribute to understanding the genetic diversity and propagation history of cattle in Central Asia.
    Wiley, Jan. 2019, Animal science journal = Nihon chikusan Gakkaiho, 90(1) (1), 29 - 34, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Fuki Kawaguchi, Hiroto Kigoshi, Ayaka Nakajima, Yuta Matsumoto, Yoshinobu Uemoto, Moriyuki Fukushima, Emi Yoshida, Eiji Iwamoto, Takayuki Akiyama, Namiko Kohama, Eiji Kobayashi, Takeshi Honda, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki
    Fatty acid composition is an important indicator of beef quality. The objective of this study was to search the potential candidate region for fatty acid composition. We performed pool-based genome-wide association studies (GWAS) for oleic acid percentage (C18:1) in a Japanese Black cattle population from the Hyogo prefecture. GWAS analysis revealed two novel candidate regions on BTA9 and BTA14. The most significant single nucleotide polymorphisms (SNPs) in each region were genotyped in a population (n=899) to verify their effect on C18:1. Statistical analysis revealed that both SNPs were significantly associated with C18:1 (p=.0080 and .0003), validating the quantitative trait loci (QTLs) detected in GWAS. We subsequently selected VNN1 and LYPLA1 genes as candidate genes from each region on BTA9 and BTA14, respectively. We sequenced full-length coding sequence (CDS) of these genes in eight individuals and identified a nonsynonymous SNP T66M on VNN1 gene as a putative candidate polymorphism. The polymorphism was also significantly associated with C18:1, but the p value (p=.0162) was higher than the most significant SNP on BTA9, suggesting that it would not be responsible for the QTL. Although further investigation will be needed to determine the responsible gene and polymorphism, our findings would contribute to development of selective markers for fatty acid composition in the Japanese Black cattle of Hyogo.
    Lead, WILEY, Aug. 2018, ANIMAL SCIENCE JOURNAL, 89(8) (8), 1060 - 1066, English
    [Refereed]
    Scientific journal

  • Ayaka Nakajima, Fuki Kawaguchi, Yoshinobu Uemoto, Moriyuki Fukushima, Emi Yoshida, Eiji Iwamoto, Takayuki Akiyama, Namiko Kohama, Eiji Kobayashi, Takeshi Honda, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki
    The objective of this study was to identify genomic regions associated with fat-related traits using a Japanese Black cattle population in Hyogo. From 1836 animals, those with high or low values were selected on the basis of corrected phenotype and then pooled into high and low groups (n = 100 each), respectively. DNA pool-based genome-wide association study (GWAS) was performed using Illumina BovineSNP50 BeadChip v2 with three replicate assays for each pooled sample. GWAS detected that two single nucleotide polymorphisms (SNPs) on BTA7 (ARS-BFGL-NGS-35463 and Hapmap23838-BTA-163815) and one SNP on BTA12 (ARS-BFGL-NGS-2915) significantly affected fat percentage (FAR). The significance of ARS-BFGL-NGS-35463 on BTA7 was confirmed by individual genotyping in all pooled samples. Moreover, association analysis between SNP and FAR in 803 Japanese Black cattle revealed a significant effect of SNP on FAR. Thus, further investigation of these regions is required to identify FAR-associated genes and mutations, which can lead to the development of DNA markers for marker-assisted selection for the genetic improvement of beef quality.
    Blackwell Publishing, May 2018, Animal Science Journal, 89(5) (5), 743 - 751, English
    [Refereed]
    Scientific journal

  • H. Kigoshi, F. Kawaguchi, R. Yasuzumi, K. Oyama, H. Mannen, S. Sasazaki
    Genetics and Molecular Research, 2018, Genetics and Molecular Research, 17(3) (3), English
    [Refereed]
    Scientific journal

  • Aoi NODA, Fuki KAWAGUCHI, Shinji SASAZAKI,, Hideyuki MANNEN
    Japanese Society of Animal Breeding and Genetics, 2018, The Journal of Animal Genetics, 46(2) (2), 49 - 55, Japanese
    [Refereed]
    Scientific journal

  • Fuki Kawaguchi, Yuto Kitamura, Ryuji Nakajima, Masayoshi Takahashi, Hiroaki Goto, Yuichi Washida, Yoshikazu Yamamoto, Shinji Sasazaki, Hideyuki Mannen
    The objective of this study was to discriminate between original Japanese and Australian Wagyu beef, which is sold in the Singapore markets, using six previously developed DNA markers. To effectively evaluate the six markers for breed identification, the probability of identification as Australian Wagyu beef was calculated based on the estimated allele frequencies using 130 Australian Wagyu individuals. The combined use of six markers would allow the discrimination of Australian Wagyu beef with an estimated probability of 0.776. The probability to discriminate Australian Wagyu from Japanese Wagyu beef was sufficiently high. In addition, Australian Wagyu has maternal mitochondrial DNA of Bos indicus cattle with moderate high frequency of 0.377. The DNA marker system could also be used as a deterrent force against false sales, and contribute to the reduction and prevention of incorrect or falsified labeling of beef.
    Lead, Blackwell Publishing, Jan. 2018, Animal Science Journal, 89(1) (1), 257 - 258, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Fuki Kawaguchi, Kazuki Okura, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki
    Previous studies have indicated that some leptin gene polymorphisms were associated with economically important traits in cattle breeds. However, polymorphisms in the leptin gene have not been reported thus far in Japanese Black cattle. Here, we aimed to identify the leptin gene polymorphisms which are associated with carcass traits and fatty acid composition in Japanese Black cattle. We sequenced the full-length coding sequence of leptin gene for eight Japanese Black cattle. Sequence comparison revealed eight single nucleotide polymorphisms (SNPs). Three of these were predicted to cause amino acid substitutions: Y7F, R25C and A80V. Then, we genotyped these SNPs in two populations (JB1 with 560 animals and JB2 with 450 animals) and investigated the effects on the traits. Y7F in JB1 and A80V in JB2 were excluded from statistical analysis because the minor allele frequencies were low (< 0.1). Association analysis revealed that Y7F had a significant effect on the dressed carcass weight in JB2 R25C had a significant effect on C18:0 and C14:1 in JB1 and JB2, respectively and A80V had a significant effect on C16:0, C16:1, C18:1, monounsaturated fatty acid and saturated fatty acid in JB1. The results suggested that these SNPs could be used as an effective marker for the improvement of Japanese Black cattle.
    Lead, Blackwell Publishing, Mar. 2017, Animal Science Journal, 88(3) (3), 433 - 438, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Fuki KAWAGUCHI, Satoshi KOGA, Kenji OYAMA, Hideyuki MANNEN,, Shinji SASAZAKI
    Lead, Japanese Society of Animal Breeding and Genetics, 2017, The Journal of Animal Genetics, 45(1) (1), 3 - 8, Japanese
    [Refereed]
    Scientific journal

  • KAWAGUCHI Fuki, FUKAZAWA Kenji, KOHAMA Namiko, AKIYAMA Takayuki, FUKUSHIMA Moriyuki, KOBAYASHI Eiji, OYAMA Kenji, MANNEN Hideyuki, SASAZAKI Shinji

    In this study, we investigated the effect of leptin gene polymorphisms on fertility traits in Japanese Black cattle of Hyogo Prefecture (n＝409). Three amino acid substitutions (Y7F, R25C, and A80V) were identified by sequence comparison of full-length coding region among 16 animals. We genotyped these substitutions for 409 animals and then analyzed the effects on three fertility traits (calving interval, age at first calving, and the number of calves produced at 4 years of age). As the result, Y7F and R25C were associated with the calving interval (P<0.05). In addition, Y7F and R25C were in strong linkage disequilibrium (r2＝0.88). However, Y7F is not so important in mature leptin because this position is cleaved off before leptin is excreted from the adipose tissue. These results suggested that the effect of Y7F on the calving interval may be due to the linkage disequilibrium with R25C and R25C would be used as effective DNA marker for the improvement of calving interval.

    Lead, Zootechnical Science Society of Japan, 2016, Nihon Chikusan Gakkaiho, 87(4) (4), 333 - 338, Japanese
    [Refereed]
    Scientific journal

■ MISC
  • 黒毛和種における全ゲノムリシーケンス解析を用いたオレイン酸含有率QTLに対する候補多型の探索
    川口芙岐
    Feb. 2020, (777) (777), 2 - 6, Japanese
    Introduction other

■ Lectures, oral presentations, etc.
  • ミトゲノム解析による東南アジア在来ヤギの伝播過程の推定
    小楠夏帆, 笹崎晋史, 川口芙岐, 田中啓介, 呉 佳齊, 米澤隆弘, 万年英之
    日本動物遺伝育種学会第25回大会, Nov. 2024, Japanese

  • ウシ6番染色体の脂肪酸組成QTLに対する責任多型の探索
    伊藤亮太朗, 吉田恵美, 岩本英治, 大山憲二, 万年英之, 笹崎晋史, 川口芙岐
    日本動物遺伝育種学会第25回大会, Nov. 2024, Japanese
    Oral presentation

  • 岩手県黒毛和種における白斑発生に関わるMITF遺伝子多型の探索
    小出毅大, 西和隆, 万年英之, 笹崎晋史, 川口芙岐
    日本畜産学会第132回大会, Sep. 2024, Japanese

  • 兵庫県黒毛和種の19番染色体における脂肪酸組成に対する責任多型の探索
    橋本 晃太郎, 小浜 菜美子, 吉田 恵実, 岩本 英治, 大山 憲二, 万年 英之, 笹崎 晋史, 川口 芙岐
    日本畜産学会第132回大会, Sep. 2024, Japanese
    Oral presentation

  • Exploring the gene polymorphisms associated with high-altitude adaptation in Nepalese goat breeds using Whole-genome resequencing
    Ryo Masuko, Natsumi Murai, Fuki Kawaguchi, Hideyuki Mannen, Shinji Sasazaki
    Plant & Animal Genome Conference: PAG 31, Jan. 2024, English

  • Comprehensive verification of all SNPs within a QTL region suggested that ICAM gene polymorphisms would be responsible for beef marbling in Japanese Black cattle
    Shinji Sasazaki, Hina Kondo, Yurika Moriishi, Fuki Kawaguchi, Kenji Oyama, Hideyuki Mannen
    Plant & Animal Genome Conference: PAG 31, Jan. 2024, English

  • The reported indel on caprine chromosome 1 is responsible for polledness in Asian goats
    Fuki Kawaguchi, Ryo Morisawa, Shinji Sasazaki, Hideyuki Mannen
    Plant & Animal Genome Conference: PAG 31, Jan. 2024, English

  • ミトゲノム解析による アジア 在来ヤギ ハプログループ B の起源および伝播経路の推定
    井上真梨, 笹崎晋史, 川口芙岐, 田中啓介, 米澤隆 弘, 呉 佳齊, 万年英之
    日本動物遺伝育種学会第 24 回大会, Nov. 2023, Japanese
    Oral presentation

  • 兵庫県黒毛和種集団を用いた 17 番染色体における 脂肪交雑 QTL の探索
    伊藤ひかり, 足立亮仁, 川口芙岐, 万年英之, 笹崎晋史
    日本動物遺伝育種学会第 24 回大会, Nov. 2023, Japanese
    Oral presentation

  • 全ゲノムリシーケンスデータを用いた ヤギ高地適応遺伝子多型の探索
    村井菜摘, 増子諒, 川口芙岐, 万年英之, 笹崎晋史
    日本動物遺伝育種学会第 24 回大会, Nov. 2023, Japanese
    Oral presentation

  • 高密度SNP マーカーを用いた東南アジア諸島在来ヤギの遺伝構造と伝播、遺伝子流入の推定
    増子諒, 高尾祐樹, アインアイン, 笹崎晋史, 川口芙岐, 山本義雄, Masangakay JS, Dagong MIA, Bugiwati SRA, Jiaqi Wu, 米澤隆弘, 万年英之
    日本畜産学会第131回大会, Sep. 2023, Japanese
    Oral presentation

  • アジア在来ヤギにおける毛色の多様性に関わるMC1R 遺伝子多型の探索
    釋天音, 川口芙岐, 万年英之, 笹崎晋史
    日本畜産学会第 131 回大会優秀発表賞応募, Sep. 2023, Japanese
    Oral presentation

  • 宮崎県黒毛和種集団を用いたウシ7番染色体における脂肪交雑に対する原因多型の探索
    森石 宥璃華, 川口 芙岐, 万年 英之, 笹崎 晋史
    日本畜産学会第131回大会, Sep. 2023, Japanese
    Oral presentation

  • Verification of candidate SNP effects reveals two QTLs on BTA7 for beef marbling in two Japanese Black cattle populations
    Shinji Sasazaki, Raito Yamamoto, Shintaro Toyomoto, Hina Kondo, Namiko Kohama, Emi Yoshida, Eiji Iwamoto, Fuki Kawaguchi, Kenji Oyama, Hideyuki Mannen
    Plant & Animal Genome Conference: PAG 30, Jan. 2023, English
    Poster presentation

  • Genome-wide association study for the percentage of oleic acid in Japanese Black cattle revealed the candidate region on BTA19
    Fuki Kawaguchi, Natsuki Maeda, Kenji Oyama, Emi Yoshida, Eiji Iwamoto, Namiko Kohama, Shinji Sasazaki, Hideyuki Mannen
    Plant & Animal Genome Conference: PAG 30, Jan. 2023, English
    Poster presentation

  • 岩手県黒毛和種における白斑とMITF遺伝子多型との関連
    金丸智香, 西和隆, 川口芙岐, 万年英之, 笹崎晋史
    日本動物遺伝育種学会第23回大会, Nov. 2022, Japanese
    Oral presentation

  • 兵庫県黒毛和種におけるゲノムワイド関連解析を用いたオレイン酸含有率QTLの探索
    前田菜月, 小浜菜美子, 秋山敬孝, 吉田恵実, 岩本英治, 大山憲二, 笹崎晋史, 万年英之, 川口芙岐
    日本畜産学会第130回大会, Sep. 2022, Japanese
    Oral presentation

  • アジア在来ヤギにおける角の有無と既報の1番染色体無角関連多型との関連
    森澤亮, 笹崎晋史, 万年英之, 川口芙岐
    日本動物遺伝育種学会第22回大会, Nov. 2021, Japanese
    Oral presentation

  • 父母系および核ゲノムSNPマーカーを用いたインドネシア在来ヤギの遺伝構造解析及び遺伝子流入の推定
    政岡真帆, 笹崎晋史, 川口芙岐, 米澤隆弘, 呉 佳齊, 山本義雄, DAGONG MIA, BUGIWATI SRA, 万年英之
    日本動物遺伝育種学会第22回大会, Nov. 2021, Japanese
    Oral presentation

  • 黒毛和種現場後代検定調査牛集団における全ゲノムリシーケンスデータを用いたオレイン酸含有率に関わる多型の探索
    緋田泰良, 笹崎晋史, 大山憲二, 万年英之, 川口芙岐
    日本畜産学会第129回大会, Sep. 2021, Japanese
    Oral presentation

  • Confirmation of quantitative trait locus location on BTA19 for the percentage of oleic acid in beef based on effects of 5 polymorphisms and linkage disequilibrium analysis in 2 Japanese Black cattle populations.
    Fuki Kawaguchi, Fuka Kakiuchi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki
    38th International Society for Animal Genetics Conference, Jul. 2021, English
    Poster presentation

  • 父母系および高密度 SNPマーカーを用いたフィリ ピン在来ヤギの遺伝構造と伝播の推定
    AYIN AYIN, 田端 里彩, 笹崎 晋史, 川口 芙岐, 小林 栄治, 山本 義雄, 米澤 隆弘, 呉 佳齊, Masangkay J, 万年 英之
    日本畜産学会第128回大会, Mar. 2021

  • 50K SNPアレイを用いた和牛4品種に関する多様性および類縁関係の調査
    中村充暉, 鈴木啓一, 小林栄治, 万年英之, 笹崎晋史, 川口芙岐
    日本畜産学会第128回大会, Mar. 2021

  • 集積流体回路を用いた兵庫県黒毛和種集団におけるロース脂肪割合候補多型の効果の検証
    近藤陽菜, 小浜菜美子, 秋山敬孝, 吉田恵実, 小林栄治, 川口芙岐, 万年英之, 笹崎晋史
    日本動物遺伝育種学会第21回大会, Nov. 2020

  • Phylogenetic analysis of Nepalese goats using mtDNA D-loop region and SRY gene sequences
    Nomura Y, Kunieda T, Shah M, Kawaguchi F, Sasazaki S, Mannen H
    37th International Society for Animal Genetics Conference, Jul. 2019, English
    Poster presentation

  • Genetic diversity of Bos taurus and Bos indicus using developed indel markers. 37th International Society for Animal Genetics Conference
    Yasui T, Yamanaka H, Kawaguchi F, Sasazaki S, Yonezawa T, Wu J, Mannen H
    37th International Society for Animal Genetics Conference, Jul. 2019, English
    Poster presentation

  • Genetic diversity and structure in 11 native Asian goat populations analyzed by high density SNP array. 37th International Society for Animal Genetics Conference
    Iso K, Sasazaki S, Kawaguchi F, Yonezawa T, Wu J, Nomura K, Takahashi Y, Kobayashi E, Shah M, Faruque O, Masangkay J.S, Bakhtin M, Kazymbet P, Dorji T, Mannen H
    37th International Society for Animal Genetics Conference, Jul. 2019, English
    Poster presentation

  • Effect of the PLAG1 gene polymorphism on carcass weight and oleic acid percentage in Japanese Black cattle populations
    Fuki KAWAGUCHI, Hiroto KIGOSHI, Riri YASUZUMI, Kenji OYAMA, Hideyuki MANNEN, Shinji SASAZAKI
    37th International Society for Animal Genetics Conference, Jul. 2019, English
    Poster presentation

  • Molecular epidemiological study of bovine leukemia virus in Zebu cattle (Bos indicus)
    Nishikaku K, Yonezawa T, Murakami S, Torii Y, Kawaguchi F, Sasazaki S, Mannen H, Kobayashi T
    The 2nd Joint Meeting of Veterinary Science in East Asia, Apr. 2019

  • アジア在来牛における牛白血病ウイルスの分子疫学的解析
    西角光平, 米澤隆弘, 村上覚史, 鳥居恭司, 川口芙岐, 刘建全, 万年英之, 小林朋子
    第54回獣医疫学会学術集会, Mar. 2019

  • mtDNA D-loop領域とSRY遺伝子の塩基配列に基づいたネパール在来ヤギの遺伝的類縁関係
    野村悠登, 国枝哲夫, Manoj Shah, 笹崎晋史, 川口芙岐, 万年英之
    日本畜産学会第125回大会, Mar. 2019, Japanese
    Oral presentation

  • 複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討
    山本雷斗, 川口芙岐, 秋山敬孝, 吉田恵実, 小浜菜美子, 小林栄治, 大山憲二, 万年英之, 笹崎晋史
    日本畜産学会第125回大会, Mar. 2019, Japanese
    Oral presentation

  • 兵庫県黒毛和種集団における全ゲノムリシーケンスデータを用いた枝肉形質に対する候補変異の抽出
    木越洸利, 川口芙岐, 大山憲二, 万年英之, 笹崎晋史
    日本動物遺伝育種学会第19回大会, Sep. 2018, Japanese
    Oral presentation

  • Detection of candidate polymorphisms in the QTL for oleic acid percentage on BTA9 based on whole-genome resequencing data
    Fuki KAWAGUCHI, Hiroto KIGOSHI, Namiko KOHAMA, Takayuki AKIYAMA, Moriyuki FUKUSHIMA, Emi YOSHIDA, Eiji KOBAYASHI, Kenji OYAMA, Hideyuki MANNEN, Shinji SASAZAKI
    Plant & Animal Genome Asia 2018, May 2018, English
    Poster presentation

  • ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索
    川口芙岐, 木越洸利, 小浜菜美子, 秋山敬孝, 福島護之, 吉田恵実, 小林栄治, 大山憲二, 万年英之, 笹崎晋史
    日本畜産学会第124回大会, Mar. 2018, Japanese
    Oral presentation

  • 兵庫県黒毛和種におけるゲノムワイド関連解析により同定されたVNN1遺伝子T66M多型のオレイン酸含有率に対する効果
    木越洸利, 川口芙岐, 上本吉伸, 福島護之, 吉田恵実, 岩本英治, 秋山敬孝, 小浜菜美子, 小林栄治, 大山憲二, 万年英之, 笹崎晋史
    日本動物遺伝育種学会第 18 回年次大会, Nov. 2017, Japanese
    Oral presentation

  • 複数の黒毛和種集団を用いたPLAG1遺伝子多型のオレイン酸含有率に対する効果の検討
    川口芙岐, 木越洸利, 安栖梨理, 小浜菜美子, 秋山敬孝, 福島護之, 小林栄治, 大山憲二, 万年英之, 笹崎晋史
    日本動物遺伝育種学会第 18 回年次大会, Nov. 2017, Japanese
    Oral presentation

  • Association of VNN1 gene polymorphism with fatty acid composition in Japanese Black cattle
    Kigoshi H, Matsumoto Y, Kawaguchi F, Uemoto Y, Kobayashi E, Fukushima M, Iwamoto E, Yoshida E, Akiyama T, Kohama N, Oyama K, Honda T, Mannen H, Sasazaki S
    36th International Society for Animal Genetics Conference, Jul. 2017, English
    Poster presentation

  • Effect of DNA markers associated with carcass traits and fatty acid composition on fertility traits in Japanese Black cows
    Tsuchimura M, Fukazawa K, Kawaguchi F, Matsuhashi T, Maruyama S, Oyama K, Sasazaki S, Mannen H
    36th International Society for Animal Genetics Conference, Jul. 2017, English
    Poster presentation

  • PLAG1 polymorphism (ss319607405) is associated with oleic acid percentage in Japanese Black cattle
    Fuki KAWAGUCHI, Hiroto KIGOSHI, Ayaka NAKAJIMA, Yuta MATSUMOTO, Yoshinobu UEMOTO, Moriyuki FUKUSHIMA, Emi YOSHIDA, Eiji IWAMOTO, Takayuki AKIYAMA, Namiko KOHAMA, Eiji KOBAYASHI, Takeshi HONDA, Kenji OYAMA, Hideyuki MANNEN, Shinji SASAZAKI
    36th International Society for Animal Genetics Conference, Jul. 2017, English
    Poster presentation

  • 黒毛和種繁殖雌牛集団におけるレプチン遺伝子内多型と繁殖形質との関連
    川口芙岐, 深澤謙治, 小浜菜美子, 秋山敬孝, 福島護之, 小林栄治, 大山憲二, 万年英之, 笹崎晋史
    日本動物遺伝育種学会第 17 回年次大会, Nov. 2016, Japanese
    Oral presentation

  • Identification of polymorphisms associated with oleic acid percentage by pool-based genome wide association study (GWAS) in Japanese Black
    Fuki KAWAGUCHI, Ayaka NAKAJIMA, Yuta MATSUMOTO, Yoshinobu UEMOTO, Moriyuki FUKUSHIMA, Emi YOSHIDA, Eiji IWAMOTO, Takayuki AKIYAMA, Namiko KOHAMA, Eiji KOBAYASHI, Takeshi HONDA, Kenji OYAMA, Shinji SASAZAKI, Hideyuki MANNEN
    35th International Society for Animal Genetics Conference, Jul. 2016, English
    Poster presentation

  • 黒毛和種集団におけるLeptin遺伝子翻訳領域内多型の脂肪酸組成に対する効果
    川口 芙岐, 大倉 一輝, 大山 憲二, 万年 英之, 笹崎 晋史
    日本畜産学会第119回大会, Mar. 2015, Japanese
    Oral presentation

■ Research Themes
  • Comprehensive Study of native Asian goats using whole genome information: New origins and migration routes of Southeast Asian Goats
    万年 英之, 川口 芙岐, 笹崎 晋史, 米澤 隆弘
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2024 - 31 Mar. 2028

  • ヒトの健康に資する牛肉生産に向けたウシ筋肉内における脂肪酸代謝機構の解明
    川口 芙岐
    日本学術振興会, 科学研究費助成事業, 若手研究, 神戸大学, 01 Apr. 2023 - 31 Mar. 2026
    本年度、兵庫県黒毛和種の全ゲノムリシーケンスデータを用い、96候補SNPsの選出と432頭集団におけるバイオマークシステムを用いたそれらの遺伝子型判定を実施した。加えて、統計解析を実施し、脂肪酸組成との関連を調査したところ、候補領域としていた35～47 Mbp全域に渡って比較的低いｐ値を示すSNPが観察された。具体的に、上位15 SNPsの選出による候補領域の絞り込みも試みたが、それら15 SNPsは領域内全体に分散しており、当初の領域より狭く有力な候補領域への絞り込みはなされなかった。しかし、現候補領域全体が候補となるべき領域であることが裏付けられ、各遺伝子の機能等調査に重点を置き、その情報に基づいて有力候補となる遺伝子の抽出を進める方針を中心とすべきであることが示された。 遺伝子の機能調査においては、NCBIやDAVID、GeneCardsなどいくつかのデータベースに登録されている各遺伝子の機能に関わる情報および先行研究から論文によって報告されている情報に基づいて、脂肪酸代謝に関わる可能性について精査した。候補領域内には約300遺伝子が位置していたが、ここでは、全ゲノムリシーケンス解析により各遺伝子内に検出されいている多型について多型間の連鎖不平衡や遺伝子内での位置に基づき、比較的易しい基準で候補多型の有無を判断し、候補多型を有する79遺伝子のみ調査対象とした。その結果、HER2、BRCA1、MLXの3遺伝子について、脂肪酸の合成や不飽和化の制御の一端を担っていると考えられ、有力な候補遺伝子であると考えられた。 本年度の成果から、候補領域の絞り込みは難しかったものの、遺伝子機能の調査によって有力な遺伝子の特定に至ったという点で責任多型の同定に大きな進展が得られた。今後、特にそれら3遺伝子内の多型に焦点を当てた分析を進め、責任多型の同定を目指す。

  • International joint research to elucidate the propagation of domestic animals across the Indian Ocean
    万年 英之, 米澤 隆弘, 下桐 猛, 笹崎 晋史, 川口 芙岐, 西堀 正英
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Kobe University, 07 Oct. 2021 - 31 Mar. 2026
    【研究の目的】本国際共同研究は「海の道」による家畜の海上伝播とその影響を明らかにすることを目的とし、着想したインド洋海上経路が中小家畜の伝播経路の一つであるという新しい仮説を検証する。その具体的方策として、インド洋海上経路の複数国（インドネシア、モーリシャス、マダガスカルなど）と国際共同研究を構築し、収集する中小家畜（ヤギ、ブタ、ニワトリ）試料に対し全ゲノムリシーケンスを含むゲノムワイドな遺伝学的解析を実施する。解析情報から広大なインド洋海路が家畜の伝播や遺伝子流入に与えた影響を評価し、ヒトの歴史に紐づけた家畜伝播の考察を行う。この解析には、家畜情報と試料（DNA）の収集という観点から、インド洋広範囲にわたる国際共同研究が必須となる。本課題により、インド洋海路に基づく新しい国際共同研究コンソーシアムを構築し、若手研究者参画によるグローバル視点を持つ将来の国際共同研究のリーダーを育成する。 【2021年度の研究実績】当該年度は世界的なコロナ禍が継続中のため、オンラインを使い国際共同研究者と調査計画を立案した。調査対象国はインドネシアとモーリシャスである。モーリシャスはFAREIのGobindram博士と連絡を取り、コロナ禍の状況を注視しながら2022年9月頃に2-3週間の調査を行う計画を立て、対象動物はヤギとニワトリである。モーリシャス本島とロドリゲス島の2島に対して調査を計画しつつある。インドネシアはHasanuddin大学のBugiwati 教授と連絡を取り、2022年度の調査計画を進めている。Lakor島、Timor島、Lembata島を対象とし、対象家畜はヤギである。コロナ禍の状況を鑑み、インドネシア共同研究者による試料収集を計画を進めている。採取された試料は冷凍保存し、機会を見て代表者が現地でのDNA精製等を行う予定としている。

  • ウシ脂肪交雑形成機構の解明に向けた候補遺伝子変異の網羅的検証
    笹崎 晋史, 川口 芙岐, 万年 英之, 大山 憲二
    日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2022 - 31 Mar. 2025

  • Have East native cattle received genetic introgression from wild cattle? -evaluation of the genetic structure and propagation route-
    万年 英之, 川口 芙岐, 笹崎 晋史, 米澤 隆弘
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2021 - 31 Mar. 2024
    本課題では、ユーラシア大陸を網羅する北方系家畜ウシ集団に対し、遺伝様式が異なるmtDNA解析、高密度SNPアレイ解析、全ゲノムリシーケンスなどによるビックデータを用いた網羅的な遺伝構造解析を実施し、その遺伝子流入、遺伝構造、起源、伝播経路などを包括的に明らかにする。その成果を通して、東アジア家畜ウシの遺伝資源保護・開発につなげ、次世代畜産分野に対する学術的知見および基礎情報を得ることを目的とする。 家畜ウシのハプログループT4は東アジア地域で特異的に観察され、特に和牛品種ではこのハプログループT4が他の東アジア地域に比べ、高頻度で観察されている。そこで本課題では、和牛品種として日本短角種と黒毛和種におけるハプログループT4のミトゲノム配列決定をすることで、ハプログループT4の系統解析や分岐年代の推定を行い、ハプログループT4の分岐・成立過程を明らかにすることを目的とした。また高密度SNPアレイを用いた解析を、和牛4品種およびアジア品種、西洋品種の遺伝子型データを得て様々な遺伝的解析を実施した。 ミトゲノム解析の結果、和牛品種は韓牛と同じクラスターに含まれ、先行研究と同様にハプログループT4はサブハプログループT3aから分岐したものであることが明らかとなった。分岐年代推定より、ハプログループT4は家畜化中心地である西アジアの肥沃な三日月地帯からシルクロードの内、温暖な環境で水が豊富に存在するオアシスの道を経て、東のアジアへと伝播する過程において生じたハプログループであると考えられた。高密度SNPアレイを用いた解析では、和牛の多様性が他の海外品種と比較して全体的に低い傾向が示された。

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