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FUJIMOTO Ryo
Graduate School of Agricultural Science / Department of Bioresource Science
Associate Professor

Researcher basic information

■ Research news
  • 20 Aug. 2019, The mechanism that controls Chinese cabbage flowering
    Link to Kobe Univ. HP
  • 27 Oct. 2016, DNA methylation affects superiority of hybrid plants -Discovery could be used in efficient breeding of high-yield vegetables-
    Link to Kobe Univ. HP
■ Research Areas
  • Environmental science/Agricultural science / Plant genetics and breeding
  • Environmental science/Agricultural science / Horticulture
■ Committee History
  • Apr. 2019 - Present, 園芸学会, The Horticulture Journal 編集委員
  • Apr. 2018 - Mar. 2020, 園芸学会, シンポジウム委員
  • 2019, Editorial Board Member for Frontiers in Plant Science
  • 2019, Editorial Board Member for Scientific Reports
  • 2018, Editorial Board Member for BMC Genomics

Research activity information

■ Award
  • Jan. 2018 神戸大学, 平成29年度優秀若手研究賞, 雑種強勢研究
    FUJIMOTO RYO
    Others

  • Oct. 2017 神戸大学農学部同窓会六篠会, 六篠論文賞, PNAS誌に掲載された論文
    FUJIMOTO RYO
    Others

  • Mar. 2017 日本育種学会, 平成28年度日本育種学会奨励賞, アブラナ科植物におけるゲノム多様性および雑種強勢に関する研究
    FUJIMOTO RYO
    Japan society

■ Paper
  • K. Tadokoro, M. Kobayashi, R. Fujimoto, T. Takasaki-Yasuda
    International Society for Horticultural Science (ISHS), Sep. 2024, Acta Horticulturae, (1404) (1404), 1051 - 1058, English
    Scientific journal

  • M.A. Akter, N. Miyaji, M. Shimizu, H. Mehraj, M.A.U. Doullah, I. Chuma, R. Fujimoto
    Corresponding, International Society for Horticultural Science (ISHS), Sep. 2024, Acta Horticulturae, (1404) (1404), 469 - 476, English
    Scientific journal

  • H. Mehraj, M.A. Akter, T. Takasaki-Yasuda, E.S. Dennis, R. Fujimoto
    International Society for Horticultural Science (ISHS), Sep. 2024, Acta Horticulturae, (1404) (1404), 323 - 330
    Scientific journal

  • Masako Mishio, Emi Sudo, Hiroshi Ozaki, Riichi Oguchi, Ryo Fujimoto, Nobuharu Fujii, Kouki Hikosaka
    Abstract Premise With the global atmospheric CO2 concentration on the rise, developing crops that can thrive in elevated CO2 has become paramount. We investigated the potential of hybridization as a strategy for creating crops with improved growth in predicted elevated atmospheric CO2. Methods We grew parent accessions and their F1 hybrids of Arabidopsis thaliana in ambient and elevated atmospheric CO2 and analyzed numerous growth traits to assess their productivity and underlying mechanisms. Results The heterotic increase in total dry mass, relative growth rate and leaf net assimilation rate was significantly greater in elevated CO2 than in ambient CO2. The CO2 response of net assimilation rate was positively correlated with the CO2 response of leaf nitrogen productivity and with that of leaf traits such as leaf size and thickness, suggesting that hybridization‐induced changes in leaf traits greatly affected the improved performance in elevated CO2. Conclusions Vegetative growth of hybrids seems to be enhanced in elevated CO2 due to improved photosynthetic nitrogen‐use efficiency compared with parents. The results suggest that hybrid crops should be well‐suited for future conditions, but hybrid weeds may also be more competitive.
    Wiley, Apr. 2024, American Journal of Botany, 111(4) (4), e16317 - e16317, English
    [Refereed]
    Scientific journal

  • Xinyu Zhao, Li Chen, Kaiwen Yuan, Yuxiang Liu, Limei Yang, Mu Zhuang, Yangyong Zhang, Yong Wang, Jialei Ji, Ryo Fujimoto, Honghao Lv
    Maximum Academic Press, 2024, Vegetable Research, 4(1) (1), e031, English
    [Refereed]
    Scientific journal

  • Ayasha Akter, Tomohiro Kakizaki, Etsuko Itabashi, Kohei Kunita, Motoki Shimizu, Mst Arjina Akter, Hasan Mehraj, Keiichi Okazaki, Elizabeth S Dennis, Ryo Fujimoto
    UNLABELLED: Brassica rapa L., which includes Chinese cabbage, turnip, and pak choi, has more complex flowering time regulation than does Arabidopsis thaliana due to the presence of multiple paralogous flowering time genes. FLOWERING LOCUS C (FLC) is one of the key genes regulating the flowering time, and B. rapa has four FLC paralogs. BrFLC5 on the reference genome is deemed a pseudogene because of a mutation (from G to A) in the splice site of the third intron, but there are some accessions with a G nucleotide in the splice site. In this study, we genotyped 310 B. rapa accessions and found that 19 had homozygous and 81 had heterozygous putative functional BrFLC5 alleles. Accessions of turnip showed the highest proportion with a functional BrFLC5 allele. BrFLC5 acts as a floral repressor when overexpressed in A. thaliana. The BrFLC5 expression level varied in pre-vernalized plants, and this transcriptional variation was not associated with the G/A polymorphism in the third intron. Three accessions having a higher BrFLC5 expression in pre-vernalized plants had a 584-bp insertion in the promoter region. Many regions homologous to this 584-bp sequence are present in the B. rapa genome, and this 584-bp inserted region has tandem duplications of an AT-rich sequence in its central region. The possibility that a high expression of a functional BrFLC5 could contribute to producing premature bolting-resistant lines in B. rapa vegetables is discussed. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11032-023-01405-0.
    Corresponding, Aug. 2023, Molecular breeding : new strategies in plant improvement, 43(8) (8), 58 - 58, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Victor Raboy, Ryo Fujimoto
    Corresponding, Frontiers Media SA, Jul. 2023, Frontiers in Plant Science, 14, 1239713, English
    [Refereed][Invited]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Md. Nuruzzaman, Mikiko Kojima, Masato Sato, Yumiko Takebayashi, Mozammel Hoque, Satoru Okamoto, Daniel J. Shea, Ryo Fujimoto, Eigo Fukai, Hitoshi Sakakibara, Keiichi Okazaki
    Elsevier BV, Jun. 2023, Scientia Horticulturae, 316, 112002 - 112002, English
    [Refereed]
    Scientific journal

  • Naomi Miyaji, Mst. Arjina Akter, Motoki Shimizu, Hasan Mehraj, Md Asad-Ud Doullah, Elizabeth S. Dennis, Izumi Chuma, Ryo Fujimoto
    Albugo candida causing white rust disease decreases the yield of Brassica rapa vegetables greatly. Resistant and susceptible cultivars in B. rapa vegetables have different immune responses against A. candida inoculation, however, the mechanism of how host plants respond to A. candida is still unknown. Using RNA-sequencing, we identified differentially expressed genes (DEGs) between A. candida inoculated [48 and 72 h after inoculation (HAI)] and non-inoculated samples in resistant and susceptible cultivars of komatsuna (B. rapa var. perviridis). Functional DEGs differed between the resistant and susceptible cultivars in A. candida inoculated samples. Salicylic acid (SA) responsive genes tended to be changed in their expression levels by A. candida inoculation in both resistant and susceptible cultivars, but different genes were identified in the two cultivars. SA-dependent systemic acquired resistance (SAR) involving genes were upregulated following A. candida inoculation in the resistant cultivar. Particular genes categorized as SAR that changed expression levels overlapped between A. candida and Fusarium oxysporum f. sp. conglutinans inoculated samples in resistant cultivar, suggesting a role for SAR in defense response to both pathogens particularly in the effector-triggered immunity downstream pathway. These findings will be useful for understanding white rust resistance mechanisms in B. rapa.
    Corresponding, May 2023, Scientific Reports, 13(1) (1), 8599 - 8599, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Saaya Shiraki, Kazumasa Fujiwara, Yoshiki Kamiya, Mst. Arjina Akter, Elizabeth S. Dennis, Ryo Fujimoto, Hasan Mehraj
    Heterosis is an important phenomenon for high-yield crop breeding and is utilized for breeding F1 varieties in horticultural crops. However, its molecular mechanism has not been elucidated, and compared to cereals, heterosis is less explored at the molecular level in horticultural crops. In this review, we compiled the new genetic and epigenetic studies on heterosis in horticultural crops. Because of the difficulty of predicting the level of heterosis from the parental genetic distance, molecular approaches are being used to study its molecular basis in horticultural crops. Transcriptome analyses in vegetables have identified photosynthesis-related genes as important in heterosis. Analysis of noncoding RNAs has suggested their involvement in regulating the heterosis of vegetative and fruit tissues. Quantitative trait locus (QTL) analysis has revealed the association of heterozygosity of a specific locus or multiple loci with heterosis of vegetative and fruit tissues. A higher level of DNA methylation was noted in the heterotic F1 of Brassica rapa leafy vegetables, while the roles of other epigenetic modifications such as histone marks have not been explored.
    Corresponding, MDPI AG, Mar. 2023, Horticulturae, 9(3) (3), 366 - 366, English
    [Refereed][Invited]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Y. Kamiya, S. Shiraki, H. Mehraj, M.A. Akter, S. Takahashi, M. Seki, E.S. Dennis, K. Osabe, R. Fujimoto
    Corresponding, International Society for Horticultural Science (ISHS), Mar. 2023, Acta Horticulturae, (1362) (1362), 65 - 70, English
    [Refereed]
    Scientific journal

  • S. Shiraki, Y. Kamiya, H. Mehraj, S. Takahashi, M. Seki, E.S. Dennis, R. Fujimoto
    Corresponding, International Society for Horticultural Science (ISHS), Mar. 2023, Acta Horticulturae, (1362) (1362), 107 - 112
    Scientific journal

  • M.A. Akter, N. Miyaji, M. Shimizu, T. Takasaki-Yasuda, E.S. Dennis, R. Fujimoto
    International Society for Horticultural Science (ISHS), Mar. 2023, Acta Horticulturae, (1362) (1362), 305 - 312
    Scientific journal

  • Long Tong, Cunbao Zhao, Jinhui Liu, Limei Yang, Mu Zhuang, Yangyong Zhang, Yong Wang, Jialei Ji, Bifeng Kuang, Kelan Tang, Zhiyuan Fang, Ryo Fujimoto, Honghao Lv
    Cabbage (Brassica oleracea var. capitata) Fusarium wilt (CFW) is a disease that poses a critical threat to global cabbage production. Screening for resistant resources in order to support the breeding of resistant cultivars is the most reliable approach to control this disease. CFW is caused by Fusarium oxysporum f. sp. conglutinans (Foc), which consists of two physiological races (race 1 and 2). While many studies have focused on resistance screening, gene mining, and inheritance-based research associated with resistance to Foc race 1, there have been few studies specifically analyzing resistance to Foc race 2, which is a potential threat that can overcome type A resistance. Here, 166 cabbage resources collected from around the world were evaluated for the resistance to both Foc races, with 46.99% and 38.55% of these cabbage lines being resistant to Foc race 1 and race 2, respectively, whereas 33.74% and 48.80% were susceptible to these two respective races. Of these 166 analyzed cabbage lines, 114 (68.67%) were found to be more susceptible to race 2 than to race 1, and 28 of them were resistant to race 1 while susceptible to race 2, underscoring the highly aggressive nature of Foc race 2. To analyze the inheritance of Foc race 2 resistance, segregated populations derived from the resistant parental line 'Badger Inbred 16' and the susceptible one '01-20' were analyzed with a major gene plus polygene mixed genetic model. The results of this analysis revealed Foc race 2-specific resistance to be under the control of two pairs of additive-dominant-epistatic major genes plus multiple additive-dominant-epistatic genes (model E). The heritability of these major genes in the BC1P1, BC1P2, and F2 generations were 32.14%, 72.80%, and 70.64%, respectively. In summary, these results may aid in future gene mining and breeding of novel CFW-resistant cabbage cultivars.
    Corresponding, Sep. 2022, Genes, 13(9) (9), English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Kaoru Tonosaki, Ryo Fujimoto, Elizabeth S Dennis, Victor Raboy, Kenji Osabe
    If food and feed production are to keep up with world demand in the face of climate change, continued progress in understanding and utilizing both genetic and epigenetic sources of crop variation is necessary. Progress in plant breeding has traditionally been thought to be due to selection for spontaneous DNA sequence mutations that impart desirable phenotypes. These spontaneous mutations can expand phenotypic diversity, from which breeders can select agronomically useful traits. However, it has become clear that phenotypic diversity can be generated even when the genome sequence is unaltered. Epigenetic gene regulation is a mechanism by which genome expression is regulated without altering the DNA sequence. With the development of high throughput DNA sequencers, it has become possible to analyze the epigenetic state of the whole genome, which is termed the epigenome. These techniques enable us to identify spontaneous epigenetic mutations (epimutations) with high throughput and identify the epimutations that lead to increased phenotypic diversity. These epimutations can create new phenotypes and the causative epimutations can be inherited over generations. There is evidence of selected agronomic traits being conditioned by heritable epimutations, and breeders may have historically selected for epiallele-conditioned agronomic traits. These results imply that not only DNA sequence diversity, but the diversity of epigenetic states can contribute to increased phenotypic diversity. However, since the modes of induction and transmission of epialleles and their stability differ from that of genetic alleles, the importance of inheritance as classically defined also differs. For example, there may be a difference between the types of epigenetic inheritance important to crop breeding and crop production. The former may depend more on longer-term inheritance whereas the latter may simply take advantage of shorter-term phenomena. With the advances in our understanding of epigenetics, epigenetics may bring new perspectives for crop improvement, such as the use of epigenetic variation or epigenome editing in breeding. In this review, we will introduce the role of epigenetic variation in plant breeding, largely focusing on DNA methylation, and conclude by asking to what extent new knowledge of epigenetics in crop breeding has led to documented cases of its successful use.
    Lead, Sep. 2022, Frontiers in plant science, 13, 958350 - 958350, English, International magazine
    [Refereed]
    Scientific journal

  • Md Nuruzzaman, Masato Sato, Satoru Okamoto, Hoque Mozammel, Daniel J Shea, Ryo Fujimoto, Motoki Shimizu, Eigo Fukai, Keiichi Okazaki
    Tuberous stem of kohlrabi is an important agronomic trait, however, the molecular basis of tuberization is poorly understood. To elucidate the tuberization mechanism, we conducted a comparative transcriptomic analysis between kohlrabi and broccoli at 10 and 20 days after germination (DAG) as tuberous stem initiated between these time points. A total of 5580 and 2866 differentially expressed transcripts (DETs) were identified between genotypes (kohlrabi vs broccoli) and growth stages (10 DAG vs 20 DAG), respectively, and most of the DETs were down-regulated in kohlrabi. Gene ontology (GO) and KEGG pathway enrichment analyses showed that the DETs between genotypes are involved in cell wall loosening and expansion, cell cycle and division, carbohydrate metabolism, hormone transport, hormone signal transduction, and in several transcription factors. The DETs identified in those categories may directly/indirectly relate to the initiation and development of tuberous stem in kohlrabi. In addition, the expression pattern of the hormone synthesis related DETs coincided with the endogenous IAA, IAAsp, GA, ABA and tZ profiles in kohlrabi and broccoli seedlings, that were revealed in our previous phytohormone analysis. This is the first report on comparative transcriptome analysis for tuberous stem formation in kohlrabi at early growth periods. The resulting data could provide significant insights into the molecular mechanism underlying tuberous stem development in kohlrabi as well as in other tuberous organ forming crops. This article is protected by copyright. All rights reserved.
    Aug. 2022, Physiologia plantarum, e13770, English, International magazine
    [Refereed]
    Scientific journal

  • Soodeh Tirnaz, Naomi Miyaji, Shohei Takuno, Philipp E. Bayer, Motoki Shimizu, Mst. Arjina Akter, David Edwards, Jacqueline Batley, Ryo Fujimoto
    DNA methylation is an epigenetic mark associated with several mechanisms in plants including immunity mechanisms. However, little is known about the regulatory role of DNA methylation in the resistance response of Brassica species against fungal diseases. White rust, caused by the fungus Albugo candida, is one of the most widespread and destructive diseases of all the cultivated Brassica species, particularly Brassica rapa L. and Brassica juncea (L.) Czern and Coss. Here, we investigate whole-genome DNA methylation modifications of B. rapa subsp. perviridis in response to white rust. As a result, 233 and 275 differentially methylated regions (DMRs) in the susceptible cultivar “Misugi” and the resistant cultivar “Nanane” were identified, respectively. In both cultivars, more than half of the DMRs were associated with genes (DMR-genes). Gene expression analysis showed that 13 of these genes were also differentially expressed between control and infected samples. Gene ontology enrichment analysis of DMR genes revealed their involvement in various biological processes including defense mechanisms. DMRs were unevenly distributed around genes in susceptible and resistant cultivars. In “Misugi,” DMRs tended to be located within genes, while in “Nanane,” DMRs tended to be located up and downstream of the genes. However, CG DMRs were predominantly located within genes in both cultivars. Transposable elements also showed association with all three sequence contexts of DMRs but predominantly with CHG and CHH DMRs in both cultivars. Our findings indicate the occurrence of DNA methylation modifications in B. rapa in response to white rust infection and suggest a potential regulatory role of DNA methylation modification in defense mechanisms which could be exploited to improve disease resistance.
    Corresponding, Frontiers Media SA, Jun. 2022, Frontiers in Plant Science, 13, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Mst. Arjina Akter, Hasan Mehraj, Naomi Miyaji, Satoshi Takahashi, Takeshi Takasaki-Yasuda, Motoaki Seki, Elizabeth S. Dennis, Ryo Fujimoto, Kenji Osabe
    Long noncoding RNAs (lncRNAs) play important roles in abiotic and biotic stress responses; however, studies on the mechanism of regulation of lncRNA expression are limited in plants. The present study examined the relationship between lncRNA expression level and two active histone modifications (H3K4me3 and H3K36me3) in Brassica rapa. Both histone marks were enriched in the chromatin regions encoding lncRNAs, especially around the transcription start site. The transcription level of long intergenic noncoding RNAs was positively associated with the level of H3K4me3 and H3K36me3, while this association was not observed in natural antisense RNAs (NATs) and intronic noncoding RNAs. As coordinate expression of mRNAs and paired NATs under biotic stress treatment has been identified, the transcriptional relationship between mRNAs and their paired NATs following Fusarium oxysporum f. sp. conglutinans (Foc) inoculation was examined. A positive association of expression levels between mRNAs and their paired NATs following Foc inoculation was observed. This association held for several defense-response-related genes and their NAT pairs. These results suggest that coordinate expression of mRNAs and paired NATs plays a role in the defense response against Foc.
    Corresponding, MDPI AG, Dec. 2021, Horticulturae, 8(1) (1), 17 - 17, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Hasan Mehraj, Satoshi Takahashi, Naomi Miyaji, Ayasha Akter, Yutaka Suzuki, Motoaki Seki, Elizabeth S. Dennis, Ryo Fujimoto
    Covalent modifications of histone proteins act as epigenetic regulators of gene expression. We report the distribution of two active histone marks (H3K4me3 and H3K36me3) in 14-day leaves in two lines of Brassica rapa L. by chromatin immunoprecipitation sequencing. Both lines were enriched with H3K4me3 and H3K36me3 marks at the transcription start site, and the transcription level of a gene was associated with the level of H3K4me3 and H3K36me3. H3K4me3- and H3K36me3-marked genes showed low tissue-specific gene expression, and genes with both H3K4me3 and H3K36me3 had a high level of expression and were constitutively expressed. Bivalent active and repressive histone modifications such as H3K4me3 and H3K27me3 marks or antagonistic coexistence of H3K36me3 and H3K27me3 marks were observed in some genes. Expression may be susceptible to changes by abiotic and biotic stresses in genes having both H3K4me3 and H3K27me3 marks. We showed that the presence of H3K36me3 marks was associated with different gene expression levels or tissue specificity between paralogous paired genes, suggesting that H3K36me3 might be involved in subfunctionalization of the subgenomes.
    Corresponding, Frontiers Media SA, Jun. 2021, Frontiers in Plant Science, 12, 659634 - 659634, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Naomi Miyaji, Mst Arjina Akter, Chizuko Suzukamo, Hasan Mehraj, Tomoe Shindo, Takeru Itabashi, Keiichi Okazaki, Motoki Shimizu, Makoto Kaji, Masahiko Katsumata, Elizabeth S. Dennis, Ryo Fujimoto
    In vegetables of Brassica rapa L., Fusarium oxysporum f. sp. rapae (For) or F. oxysporum f. sp. conglutinans (Foc) cause Fusarium yellows. A resistance gene against Foc (FocBr1) has been identified, and deletion of this gene results in susceptibility (focbr1-1). In contrast, a resistance gene against For has not been identified. Inoculation tests showed that lines resistant to Foc were also resistant to For, and lines susceptible to Foc were susceptible to For. However, prediction of disease resistance by a dominant DNA marker on FocBr1 (Bra012688m) was not associated with disease resistance of For in some komatsuna lines using an inoculation test. QTL-seq using four F2 populations derived from For susceptible and resistant lines showed one causative locus on chromosome A03, which covers FocBr1. Comparison of the amino acid sequence of FocBr1 between susceptible and resistant alleles (FocBr1 and FocBo1) showed that six amino acid differences were specific to susceptible lines. The presence and absence of FocBr1 is consistent with For resistance in F2 populations. These results indicate that FocBr1 is essential for For resistance, and changed amino acid sequences result in susceptibility to For. This susceptible allele is termed focbr1-2, and a new DNA marker (focbr1-2m) for detection of the focbr1-2 allele was developed.
    Corresponding, MDPI AG, May 2021, Plants, 10(6) (6), 1082 - 1082, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Naomi Miyaji, Motoki Shimizu, Takeshi Takasaki-Yasuda, Elizabeth S Dennis, Ryo Fujimoto
    KEY MESSAGE: Fusarium yellows resistant and susceptible lines in Brassica rapa showed different salicylic acid responses; the resistant line showed a similar response to previous reports, but the susceptible line differed. Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans (Foc) is an important disease. Previous studies showed that genes related to salicylic acid (SA) response were more highly induced following Foc infection in Brassica rapa Fusarium yellows resistant lines than susceptible lines. However, SA-induced genes have not been identified at the whole genome level and it was unclear whether they were up-regulated by Foc inoculation. Transcriptome analysis with and without SA treatment in the B. rapa Fusarium yellows susceptible line 'Misugi' and the resistant line 'Nanane' was performed to obtain insights into the relationship between SA sensitivity/response and Fusarium yellows resistance. 'Nanane's up-regulated genes were related to SA response and down-regulated genes were related to jasmonic acid (JA) or ethylene (ET) response, but differentially expressed genes in 'Misugi' were not. This result suggests that Fusarium yellows resistant and susceptible lines have a different SA response and that an antagonistic transcription between SA and JA/ET responses was found only in a Fusarium yellows resistant line. SA-responsive genes were induced by Foc inoculation in Fusarium yellows resistant (RJKB-T23) and susceptible lines (RJKB-T24). By contrast, 39 SA-induced genes specific to RJKB-T23 might function in the defense response to Foc. In this study, SA-induced genes were identified at the whole genome level, and the possibility, the defense response to Foc observed in a resistant line could be mediated by SA-induced genes, is suggested. These results will be useful for future research concerning the SA importance in Foc or other diseases resistance in B. rapa.
    Corresponding, Apr. 2021, Plant cell reports, 40(4) (4), 605 - 619, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Hasan Mehraj, Daniel J Shea, Satoshi Takahashi, Naomi Miyaji, Ayasha Akter, Motoaki Seki, Elizabeth S Dennis, Ryo Fujimoto, Kenji Osabe
    Long noncoding RNAs (lncRNAs) are RNA fragments that generally do not code for a protein but are involved in epigenetic gene regulation. In this study, lncRNAs of Brassica rapa were classified into long intergenic noncoding RNAs, natural antisense RNAs, and intronic noncoding RNAs and their expression analyzed in relation to genome-wide 24-nt small interfering RNAs (siRNAs), DNA methylation, and histone H3 lysine 27 trimethylation marks (H3K27me3). More than 65% of the lncRNAs analyzed consisted of one exon, and more than 55% overlapped with inverted repeat regions (IRRs). Overlap of lncRNAs with IRRs or genomic regions encoding for 24-nt siRNAs resulted in increased DNA methylation levels when both were present. LncRNA did not overlap greatly with H3K27me3 marks, but the expression level of intronic noncoding RNAs that did coincide with H3K27me3 marks was higher than without H3K27me3 marks. The Brassica genus comprises important vegetables and oil seed crops grown across the world. B. rapa is a diploid (AA genome) thought to be one of the ancestral species of both B. juncea (AABB genome) and B. napus (AACC) through genome merging (allotetrapolyploidization). Complex genome restructuring and epigenetic alterations are thought to be involved in these allotetrapolyploidization events. Comparison of lncRNAs between B. rapa and B. nigra, B. oleracea, B. juncea, and B. napus showed the highest conservation with B. oleracea. This study presents a comprehensive analysis of the epigenome structure of B. rapa at multi-epigenetic levels (siRNAs, DNA methylation, H3K27me3, and lncRNAs) and identified a suite of candidate lncRNAs that may be epigenetically regulated in the Brassica genus.
    2021, PloS one, 16(3) (3), e0242530, English, International magazine
    [Refereed]
    Scientific journal

  • Hasan Mehraj, Ayasha Akter, Naomi Miyaji, Junji Miyazaki, Daniel J Shea, Ryo Fujimoto, Md Asad-Ud Doullah
    The genus Brassica contains important vegetable crops, which serve as a source of oil seed, condiments, and forages. However, their production is hampered by various diseases such as clubroot and Fusarium wilt, especially in Brassica vegetables. Soil-borne diseases are difficult to manage by traditional methods. Host resistance is an important tool for minimizing disease and many types of resistance (R) genes have been identified. More than 20 major clubroot (CR) disease-related loci have been identified in Brassica vegetables and several CR-resistant genes have been isolated by map-based cloning. Fusarium wilt resistant genes in Brassica vegetables have also been isolated. These isolated R genes encode the toll-interleukin-1 receptor/nucleotide-binding site/leucine-rice-repeat (TIR-NBS-LRR) protein. DNA markers that are linked with disease resistance allele have been successfully applied to improve disease resistance through marker-assisted selection (MAS). In this review, we focused on the recent status of identifying clubroot and Fusarium wilt R genes and the feasibility of using MAS for developing disease resistance cultivars in Brassica vegetables.
    Jun. 2020, Plants (Basel, Switzerland), 9(6) (6), English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Hasan Mehraj;Takahiro Kawanabe; Motoki Shimizu; Naomi Miyaji; Ayasha Akter; Elizabeth S. Dennis; Ryo Fujimoto
    Hasan Mehraj, Takahiro Kawanabe, Motoki Shimizu, Naomi Miyaji, Ayasha Akter, Elizabeth S. Dennis, Ryo Fujimoto
    Corresponding, Mar. 2020, Plants, 9(4) (4), 414
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Ayasha Akter, Etsuko Itabashi, Tomohiro Kakizaki, Keiichi Okazaki, Elizabeth S Dennis, Ryo Fujimoto
    The genus Brassica includes oil crops, vegetables, condiments, fodder crops, and ornamental plants. Brassica species underwent a whole genome triplication event after speciation between ancestral species of Brassica and closely related genera including Arabidopsis thaliana. Diploid species such as Brassica rapa and Brassica oleracea have three copies of genes orthologous to each A. thaliana gene, although deletion in one or two of the three homologs has occurred in some genes. The floral transition is one of the crucial events in a plant's life history, and time of flowering is an important agricultural trait. There is a variation in flowering time within species of the genus Brassica, and this variation is largely dependent on a difference in vernalization requirements. In Brassica, like in A. thaliana, the key gene of vernalization is FLOWERING LOCUS C (FLC). In Brassica species, the vernalization response including the repression of FLC expression by cold treatment and the enrichment of the repressive histone modification tri-methylated histone H3 lysine 27 (H3K27me3) at the FLC locus is similar to A. thaliana. B. rapa and B. oleracea each have four paralogs of FLC, and the allotetraploid species, Brassica napus, has nine paralogs. The increased number of paralogs makes the role of FLC in vernalization more complicated; in a single plant, paralogs vary in the expression level of FLC before and after vernalization. There is also variation in FLC expression levels between accessions. In this review, we focus on the regulatory circuits of the vernalization response of FLC expression in the genus Brassica.
    Corresponding, 2020, Frontiers in plant science, 11, 619417 - 619417, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Ayasha Akter, Junji Miyazaki, Daniel J. Shea, Namiko Nishida, Satoko Takada, Naomi Miyaji, Hasan Mehraj, Motoki Shimizu, Md. Asad-ud Doullah, Takeshi Takasaki-Yasuda, Keiichi Okazaki, Ryo Fujimoto
    Last, Japanese Society for Horticultural Science, 2020, The Horticulture Journal, 89(3) (3), 268 - 277, English
    [Refereed]
    Scientific journal

  • Comparison of Cold Responses for Orthologs of Cabbage Vernalization-related Genes
    Etsuko Itabashi, Daniel J. Shea, Nobuko Fukino, Ryo Fujimoto, Keiichi Okazaki, Tomohiro Kakizaki, Takayoshi Ohara
    Oct. 2019, The Horticulture Journal, 88(4) (4), 462 - 470, English
    [Refereed]
    Scientific journal

  • Ayasha Akter, Satoshi Takahashi, Weiwei Deng, Daniel J Shea, Etsuko Itabashi, Motoki Shimizu, Naomi Miyaji, Kenji Osabe, Namiko Nishida, Yutaka Suzuki, Chris A Helliwell, Motoaki Seki, William James Peacock, Elizabeth S Dennis, Ryo Fujimoto
    Brassica rapa L. is an important vegetable and oilseed crop. We investigated the distribution of the histone mark tri-methylation of H3K27 (H3K27me3) in B. rapa and its role in the control of gene expression at two stages of development (2-day cotyledons and 14-day leaves) and among paralogs in the triplicated genome. H3K27me3 has a similar distribution in two inbred lines, while there was variation of H3K27me3 sites between tissues. Sites that are specific to 2-day cotyledons have increased transcriptional activity, and low levels of H3K27me3 in the gene body region. In 14-day leaves, levels of H3K27me3 were associated with decreased gene expression. In the triplicated genome, H3K27me3 is associated with paralogs that have tissue-specific expression. Even though B. rapa and Arabidopsis thaliana are not closely related within the Brassicaceae, there is conservation of H3K27me3-marked sites in the two species. Both B. rapa and A. thaliana require vernalization for floral initiation with FLC being the major controlling locus. In all four BrFLC paralogs, low-temperature treatment increases H3K27me3 at the proximal nucleation site reducing BrFLC expression. Following return to normal temperature growth conditions, H3K27me3 spreads along all four BrFLC paralogs providing stable repression of the gene.
    Oct. 2019, DNA research : an international journal for rapid publication of reports on genes and genomes, 26(5) (5), 433 - 443, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Satoko Takada, Ayasha Akter, Etsuko Itabashi, Namiko Nishida, Daniel J Shea, Naomi Miyaji, Hasan Mehraj, Kenji Osabe, Motoki Shimizu, Takeshi Takasaki-Yasuda, Tomohiro Kakizaki, Keiichi Okazaki, Elizabeth S Dennis, Ryo Fujimoto
    There is a wide variation of flowering time among lines of Brassica rapa L. Most B. rapa leafy (Chinese cabbage etc.) or root (turnip) vegetables require prolonged cold exposure for flowering, known as vernalization. Premature bolting caused by low temperature leads to a reduction in the yield/quality of these B. rapa vegetables. Therefore, high bolting resistance is an important breeding trait, and understanding the molecular mechanism of vernalization is necessary to achieve this goal. In this study, we demonstrated that BrFRIb functions as an activator of BrFLC in B. rapa. We showed a positive correlation between the steady state expression levels of the sum of the BrFLC paralogs and the days to flowering after four weeks of cold treatment, suggesting that this is an indicator of the vernalization requirement. We indicate that BrFLCs are repressed by the accumulation of H3K27me3 and that the spreading of H3K27me3 promotes stable FLC repression. However, there was no clear relationship between the level of H3K27me3 in the BrFLC and the vernalization requirement. We also showed that if there was a high vernalization requirement, the rate of repression of BrFLC1 expression following prolonged cold treatments was lower.
    Sep. 2019, Scientific reports, 9(1) (1), 13843 - 13843, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Allele specific DNA marker for fusarium resistance gene FocBo1 in Brassica oleracea
    Maho Sato, Motoki Shimizu, Daniel J. Shea, Mozammel Hoque, Takahiro Kawanabe, Naomi Miyaji, FUJIMOTO RYO, Eigo Fukai, Keiichi Okazaki
    Jul. 2019, Breeding Science, 69, 308 - 315, English
    [Refereed]
    Scientific journal

  • Daniel J Shea, Namiko Nishida, Satoko Takada, Etsuko Itabashi, Satoshi Takahashi, Ayasha Akter, Naomi Miyaji, Kenji Osabe, Hasan Mehraj, Motoki Shimizu, Motoaki Seki, Tomohiro Kakizaki, Keiichi Okazaki, Elizabeth S Dennis, Ryo Fujimoto
    Brassica rapa L. is an important agricultural crop that requires a period of prolonged cold for flowering. This process is known as vernalization. Studies have shown that long noncoding RNAs (lncRNAs) play important roles in abiotic stress responses and several cold-responsive noncoding RNAs have been suggested to be involved in vernalization. We examined the transcriptome of the Chinese cabbage inbred line (B. rapa L. var. pekinensis) RJKB-T24, and identified 1,444 long intergenic noncoding RNAs (lincRNAs), 551 natural antisense transcripts (NATs), and 93 intronic noncoding RNAs (incRNAs); 549 of the 2,088 lncRNAs significantly altered their expression in response to four weeks of cold treatment. Most differentially expressed lncRNAs did not lead to a change of expression levels in mRNAs covering or near lncRNAs, suggesting that the transcriptional responses to four weeks of cold treatment in lncRNA and mRNA are independent. However, some differentially expressed mRNAs had NATs with expression altered in the same direction. These genes were categorized as having an abiotic stress response, suggesting that the paired-expression may play a role in the transcriptional response to vernalization or cold treatment. We also identified short-term cold treatment induced NATs in BrFLC and BrMAF genes, which are involved in vernalization. The lncRNAs we identified differed from those reported in Arabidopsis thaliana, suggesting the role of lncRNAs in vernalization differ between these two species.
    Jun. 2019, Scientific reports, 9(1) (1), 9302 - 9302, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • 土松 隆志, 安田 晋輔, 高田 美信, 北柴 大泰, 新倉 聡, FUJIMOTO RYO, 柿崎 智博
    Japanese Society of Breeding, Jun. 2019, 育種学研究, 21(1) (1), 61 - 68, Japanese

  • Genome-Wide Analysis of Parent-of-Origin Allelic Expression in Endosperms of Brassicaceae Species, Brassica rapa.
    Takanori Yoshida, Takahiro Kawanabe, Yina Bo, FUJIMOTO RYO, Akira Kawabe
    Dec. 2018, Plant & Cell Physiology, 59(12) (12), 2590 - 2601, English
    [Refereed]
    Scientific journal

  • Satoshi Takahashi, Kenji Osabe, Naoki Fukushima, Shohei Takuno, Naomi Miyaji, Motoki Shimizu, Takeshi Takasaki-Yasuda, Yutaka Suzuki, Elizabeth S Dennis, Motoaki Seki, Ryo Fujimoto
    Epigenetic gene regulation is crucial to plant life and can involve dynamic interactions between various histone modifications, DNA methylation, and small RNAs. Detailed analysis of epigenome information is anticipated to reveal how the DNA sequence of the genome is translated into the plant's phenotype. The aim of this study was to map the DNA methylation state at the whole genome level and to clarify the relationship between DNA methylation and transcription, small RNA expression, and histone H3 lysine 9 di-methylation (H3K9me2) in Brassica rapa. We performed whole genome bisulfite sequencing, small RNA sequencing, and chromatin immunoprecipitation sequencing using H3K9me2 antibody in a Chinese cabbage inbred line, RJKB-T24, and examined the impact of epigenetic states on transcription. Cytosine methylation in DNA was analysed in different sequence contexts (CG, CHG, and CHH) (where H could be A, C, or T) and position (promoter, exon, intron, terminator, interspersed repeat regions), and the H3K9me2 and 24 nucleotide small interfering RNAs (24 nt-siRNA) were overlaid onto the B. rapa reference genome. The epigenome was compared with that of Arabidopsis thaliana and the relationship between the position of DNA methylation and gene expression, and the involvement of 24 nt siRNAs and H3K9me2 are discussed.
    Oct. 2018, DNA research : an international journal for rapid publication of reports on genes and genomes, 25(5) (5), 511 - 520, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • How Brassica oleracea FLOWERING LOCUS C 2 works in Brassica rapa genetic background
    Namiko Nishida, Daniel J. Shea, Keiichi Okazaki, FUJIMOTO RYO
    Sep. 2018, AusCanola 2018 Proceedings E-book, 127 - 131, English
    [Invited]

  • Characterization of FLOWERING LOCUS C genes in leafy Brassica rapa vegetables
    FUJIMOTO RYO, Ayasha Akter, Namiko Nishida, Takeshi Takasaki-Yasuda, Elizabeth S Dennis
    Sep. 2018, AusCanola 2018 Proceedings E-book, 71 - 75, English
    [Invited]

  • The production and characterization of a BoFLC2 introgressed Brassica rapa by repeated backcrossing to an F1
    Daniel J. Shea, Yuki Tomaru, Etsuko Itabashi, Yuri Nakamura, Toshio Miyazaki, Tomohiro Kakizaki, Tonu Nazmoon Naher, Motoki Shimizu, FUJIMOTO RYO, Eigo Fukai, Keiichi Okazaki
    Aug. 2018, Breeding Science, 68(3) (3), 316 - 325, English
    [Refereed]
    Scientific journal

  • Genome re-sequencing, SNP analysis, and genetic mapping of the parental lines of a commercial F1 hybrid cultivar of Chinese cabbage
    Daniel J. Shea, Motoki Shimizu, Etsuko Itabashi, Naomi Miyaji, Junji Miyazaki, Kenji Osabe, Makoto Kaji, Keiichi Okazaki, FUJIMOTO RYO
    Aug. 2018, Breeding Science, 68(3) (3), 375 - 380, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Naomi Miyaji, Ryo Fujimoto
    Academic Press Inc., Jan. 2018, Advances in Botanical Research, 88, 247 - 275, English
    Scientific journal

  • Daniel J. Shea, Etsuko Itabashi, Satoko Takada, Eigo Fukai, Tomohiro Kakizaki, Ryo Fujimoto, Keiichi Okazaki
    CSIRO, 2018, Crop and Pasture Science, 69(1) (1), 30 - 39, English
    [Refereed]
    International conference proceedings
    Link to Kobe Univ. Repository (Kernel)

  • Satoshi Takahashi, Naoki Fukushima, Kenji Osabe, Etsuko Itabashi, Motoki Shimizu, Naomi Miyaji, Takeshi Takasaki-Yasuda, Yutaka Suzuki, Motoaki Seki, Ryo Fujimoto
    CSIRO, 2018, Crop and Pasture Science, 69(1) (1), 107 - 120, English
    [Refereed]
    International conference proceedings
    Link to Kobe Univ. Repository (Kernel)

  • Etsuko Itabashi, Kenji Osabe, Ryo Fujimoto, Tomohiro Kakizaki
    Springer Verlag, Jan. 2018, Plant Cell Reports, 37(1) (1), 87 - 101, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Ryo Fujimoto, Kosuke Uezono, Wsonoko Ishikura, Kenji Osabe, W. James Peacock, Elizabeth S. Dennis
    Japanese Society of Breeding, 2018, Breeding Science, 68(2) (2), 145 - 158, English
    [Refereed][Invited]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Daniel J. Shea, Motoki Shimizu, Namiko Nishida, Eigo Fukai, Takashi Abe, Ryo Fujimoto, Keiichi Okazaki
    Dec. 2017, BMC GENETICS, 18, 101, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Naomi Miyaji, Motoki Shimizu, Junji Miyazaki, Kenji Osabe, Maho Sato, Yusuke Ebe, Satoko Takada, Makoto Kaji, Elizabeth S. Dennis, Ryo Fujimoto, Keiichi Okazaki
    Dec. 2017, PLANT CELL REPORTS, 36(12) (12), 1841 - 1854, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • FUJIMOTO RYO
    Japanese Society of Breeding, Sep. 2017, 育種学研究, 19(3) (3), 116 - 123, Japanese
    [Invited]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Mozammel Hoque, Daniel J. Shea, Mitsuru Asada, Md. Asad-ud-Doullah, Motoki Shimizu, Ryo Fujimoto, Eigo Fukai, Keiichi Okazaki
    Sep. 2017, MOLECULAR BREEDING, 37(9) (9), 109, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • 山本 雅也, 藤井 壮太, 柿崎 智博, FUJIMOTO RYO, 彦坂 幸毅, 畠山 勝徳, 伊藤 秀臣, 岡崎 桂一
    日本育種学会, Apr. 2017, 育種学研究, 19(1) (1), 21 - 29, Japanese
    [Invited]
    Link to Kobe Univ. Repository (Kernel)

  • Kazutaka Kawamura, Motoki Shimizu, Takahiro Kawanabe, Zujing Pu, Taro Kodama, Makoto Kaji, Kenji Osabe, Ryo Fujimoto, Keiichi Okazaki
    Jan. 2017, EUPHYTICA, 213(1) (1), 28, English
    [Refereed]
    Scientific journal

  • Takahiro Kawanabe, Sonoko Ishikura, Naomi Miyaji, Taku Sasaki, Li Min Wu, Etsuko Itabashi, Satoko Takada, Motoki Shimizu, Takeshi Takasaki-Yasuda, Kenji Osabe, W. James Peacock, Elizabeth S. Dennis, Ryo Fujimoto
    Oct. 2016, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 113(43) (43), E6704 - E6711, English
    [Refereed]
    Scientific journal

  • Genome-wide analysis of DNA methylation in Chinese cabbage
    Naoki Fukushima, Satoshi Takahashi, Etsuko Itabashi, Motoki Shimizu, Kenji Osabe, Takeshi Yasuda, Yutaka Suzuki, Motoaki Seki, Elizabeth S. Dennis, FUJIMOTO RYO
    Oct. 2016, Brassica 2016 Abstract Book, 172 - 177, English
    International conference proceedings

  • Identification of the Fusarium yellows resistance genes; its application for marker-assisted selection in Brassica rapa
    Naomi Miyaji, Motoki Shimizu, Satoko Takada, Takeshi Takasaki-Yasuda, Makoto Kaji, Kenji Osabe, Elizabeth S. Dennis, Keiichi Okazaki, FUJIMOTO RYO
    Oct. 2016, Brassica 2016 Abstract Book, 185 - 190, English

  • The epigenetic regulation of FLC expression by vernalization in Brassica rapa L
    Satoko Takada, Etsuko Itabashi, Naomi Miyaji, Takeshi Takasaki-Yasuda, Kenji Osabe, Elizabeth S. Dennis, FUJIMOTO RYO
    Oct. 2016, Brassica 2016 Abstract Book, 220 - 225, English

  • Characterization of early developmental and yield heterosis in Chinese cabbage
    FUJIMOTO RYO, Motoki Shimizu, Naomi Miyaji, Satoko Takada, Naoki Fukushima, Makoto Kaji, W. James Peacock, Elizabeth S. Dennis
    Oct. 2016, Brassica 2016 Abstract Book, 166 - 171, English

  • Kaoru Tonosaki, Kenji Osabe, Takahiro Kawanabe, Ryo Fujimoto
    Jun. 2016, BREEDING SCIENCE, 66(3) (3), 333 - 349, English
    [Refereed]
    Scientific journal

  • Ultrastructural Changes in the stigma and upper style during floral development of Japanese pear.
    Li Lily, Okamura Ayaha, Nakanishi Tetsu, Fujimoto Ryo, TAKASAKI-YASUDA TAKESHI
    医学生物学電子顕微鏡技術学会, Mar. 2016, J. Electr. Microsc.Technol. Med. Biol., 29(2) (2), 1 - 7, English
    [Refereed]
    Scientific journal

  • Kazutaka Kawamura, Takahiro Kawanabe, Motoki Shimizu, Atsushi J. Nagano, Natsumi Saeki, Keiichi Okazaki, Makoto Kaji, Elizabeth S. Dennis, Kenji Osabe, Ryo Fujimoto
    Elsevier B.V., Mar. 2016, Plant Gene, 5, 1 - 7, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Kazutaka Kawamura, Takahiro Kawanabe, Motoki Shimizu, Keiichi Okazaki, Makoto Kaji, Elizabeth S. Dennis, Kenji Osabe, Ryo Fujimoto
    Elsevier Inc., Mar. 2016, Data in Brief, 6(6) (6), 229 - 237, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Natsumi Saeki, Takahiro Kawanabe, Hua Ying, Motoki Shimizu, Mikiko Kojima, Hiroshi Abe, Keiichi Okazaki, Makoto Kaji, Jennifer M. Taylor, Hitoshi Sakakibara, W. James Peacock, Elizabeth S. Dennis, Ryo Fujimoto
    Feb. 2016, BMC PLANT BIOLOGY, 16(1) (1), 45, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Zijing Pu, Yoko Ino, Yayoi Kimura, Asumi Tago, Motoki Shimizu, Satoshi Natsume, Yoshitaka Sano, Ryo Fujimoto, Kentaro Kaneko, Daniel J. Shea, Eigo Fukai, Shin-Ichi Fuji, Hisashi Hirano, Keiichi Okazaki
    Feb. 2016, FRONTIERS IN PLANT SCIENCE, 7, 31, English
    [Refereed]
    Scientific journal

  • Takahiro Kawanabe, Kenji Osabe, Etsuko Itabashi, Keiichi Okazaki, Elizabeth S. Dennis, Ryo Fujimoto
    Feb. 2016, GENES & GENETIC SYSTEMS, 91(1) (1), 1 - 10, English
    [Refereed]
    Scientific journal

  • Motoki Shimizu, Zi-jing Pu, Takahiro Kawanabe, Hiroyasu Kitashiba, Satoru Matsumoto, Yusuke Ebe, Monari Sano, Taketo Funaki, Eigo Fukai, Ryo Fujimoto, Keiichi Okazaki
    Jan. 2015, THEORETICAL AND APPLIED GENETICS, 128(1) (1), 119 - 130, English
    [Refereed]
    Scientific journal

  • Motoki Shimizu, Ryo Fujimoto, Hua Ying, Zi-jing Pu, Yusuke Ebe, Takahiro Kawanabe, Natsumi Saeki, Jennifer M. Taylor, Makoto Kaji, Elizabeth S. Dennis, Keiichi Okazaki
    Jun. 2014, PLANT MOLECULAR BIOLOGY, 85(3) (3), 247 - 257, English
    [Refereed]
    Scientific journal

  • Yuji Kishima, Ryohei Terauchi, Yoshinobu Takada, TAKUMI SHIGEO, FUJIMOTO RYO, Koji Murai
    日本育種学会, Jun. 2014, 育種学研究, 16(2) (2), 79 - 85, Japanese
    [Invited]

  • Md. Masud Karim, Asfakun Siddika, Nazmoon Naher Tonu, Delwar M. Hossain, Md. Bahadur Meah, Takahiro Kawanabe, Ryo Fujimoto, Keiichi Okazaki
    Jan. 2014, BREEDING SCIENCE, 63(5) (5), 495 - 502, English
    [Refereed]
    Scientific journal

  • Hiroya Tomita, Motoki Shimizu, Md Asad-ud Doullah, Ryo Fujimoto, Keiichi Okazaki
    Dec. 2013, MOLECULAR BREEDING, 32(4) (4), 889 - 900, English
    [Refereed]
    Scientific journal

  • Michael Groszmann, Ian K. Greaves, Ryo Fujimoto, W. James Peacock, Elizabeth S. Dennis
    Dec. 2013, TRENDS IN GENETICS, 29(12) (12), 684 - 690, English
    [Refereed]
    Scientific journal

  • Comparison of Positions of QTLs Conferring Resistance to Xanthomonas campestris pv. campestris in Brassica oleracea
    TONU NAZMOON NAHER, DOULLAH MD. ASAD-UD, SHIMIZU MOTOKI, KARIM MD. MASUD, KAWANABE TAKAHIRO, FUJIMOTO RYO, OKAZAKI KEIICHI
    Black rot, caused by Xanthomonas campestris pv. campestris (Xcc) is possibly the most important disease of Brassica worldwide. To compare chromosomal positions of Xcc resistance loci in Brassica oleracea between the present and published studies and to develop marker assisted selection (MAS) to resistance against Xcc race 1, we constructed a B. oleracea map, including pW, pX an
    Scientific Research, Aug. 2013, American Journal of Plant Sciences, 4(8) (8), 11 - 20, English
    [Refereed]
    Scientific journal

  • Osabe K, Sasaki T, Ishikawa R, Fujimoto R
    2013, DNA Methylation: Principles, Mechanisms and Challenges
    [Refereed]

  • Takahiro Kawanabe, Ryo Fujimoto, Taku Sasaki, Jennifer M. Taylor, Elizabeth S. Dennis
    Sep. 2012, GENE, 506(2) (2), 301 - 309, English
    [Refereed][Invited]
    Scientific journal

  • Zi-jing Pu, Motoki Shimizu, Yan-ju Zhang, Tomohiko Nagaoka, Takeshi Hayashi, Hidetaka Hori, Satoru Matsumoto, Ryo Fujimoto, Keiichi Okazaki
    Aug. 2012, MOLECULAR BREEDING, 30(2) (2), 809 - 818, English
    [Refereed]
    Scientific journal

  • Ryo Fujimoto, Taku Sasaki, Ryo Ishikawa, Kenji Osabe, Takahiro Kawanabe, Elizabeth S. Dennis
    Aug. 2012, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 13(8) (8), 9900 - 9922, English
    [Refereed][Invited]
    Scientific journal

  • Kenji Osabe, Takahiro Kawanabe, Taku Sasaki, Ryo Ishikawa, Keiichi Okazaki, Elizabeth S. Dennis, Tomohiko Kazama, Ryo Fujimoto
    Jul. 2012, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 13(7) (7), 8696 - 8721, English
    [Refereed][Invited]
    Scientific journal

  • Ryo Fujimoto, Jennifer M. Taylor, Sachiko Shirasawa, W. James Peacock, Elizabeth S. Dennis
    May 2012, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(18) (18), 7109 - 7114, English
    [Refereed][Invited]
    Scientific journal

  • Kawanabe T, Osabe K, Fujimoto R
    2012, Bulletin of the Faculty of Agriculture, Niigata University, 64(2) (2), 143 - 149
    [Refereed]

  • Ryo Fujimoto, Jennifer M. Taylor, Taku Sasaki, Takahiro Kawanabe, Elizabeth S. Dennis
    Nov. 2011, PLANT MOLECULAR BIOLOGY, 77(4-5) (4-5), 419 - 431, English
    [Refereed]
    Scientific journal

  • Takahiro Kawanabe, Ryo Fujimoto
    Oct. 2011, PLANT SCIENCE, 181(4) (4), 496 - 503, English
    [Refereed]
    Scientific journal

  • Michael Groszmann, Ian K. Greaves, Nicolas Albert, Ryo Fujimoto, Chris A. Helliwell, Elizabeth S. Dennis, W. James Peacock
    Aug. 2011, BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, 1809(8) (8), 427 - 437, English
    [Refereed]
    Scientific journal

  • Ryo Fujimoto, Taku Sasaki, Hiroshi Kudoh, Jennifer M. Taylor, Tetsuji Kakutani, Elizabeth S. Dennis
    Jun. 2011, PLANT JOURNAL, 66(5) (5), 831 - 843, English
    [Refereed]
    Scientific journal

  • Taku Sasaki, Ryo Fujimoto, Sachie Kishitani, Takeshi Nishio
    Jan. 2011, PLANT CELL REPORTS, 30(1) (1), 81 - 88, English
    [Refereed]
    Scientific journal

  • Ryo Fujimoto, Yuki Kinoshita, Akira Kawabe, Tetsu Kinoshita, Kazuya Takashima, Magnus Nordborg, Mikhail E. Nasrallah, Kentaro K. Shimizu, Hiroshi Kudoh, Tetsuji Kakutani
    Apr. 2008, PLOS GENETICS, 4(4) (4), English
    [Refereed]
    Scientific journal

  • Ryo Fujimoto, Yuki Kinoshita, Akira Kawabe, Tetsu Kinoshita, Kazuya Takashima, Magnus Nordborg, Mikhail E. Nasrallah, Kentaro K. Shimizu, Hiroshi Kudoh, Tetsuji Kakutani
    Apr. 2008, PLoS Genetics, 4(4) (4), e1000048, English
    [Refereed]
    Scientific journal

  • Ryo Fujimoto, Taku Sasaki, Hisashi Inoue, Takeshi Nishio
    Mar. 2008, PLANT MOLECULAR BIOLOGY, 66(5) (5), 463 - 473, English
    [Refereed]
    Scientific journal

  • The pattern of amplification and differentiation of Ty1-copia and Ty3-gypsy retrotransposons in Brassicaceae species
    Ryo Fujimoto, Shohei Takuno, Taku Sasaki, Takeshi Nishio
    Feb. 2008, GENES & GENETIC SYSTEMS, 83(1) (1), 13 - 22, English
    [Refereed]
    Scientific journal

  • Akira Kawabe, Ryo Fujimoto, Deborah Charlesworth
    Nov. 2007, CURRENT BIOLOGY, 17(21) (21), 1885 - 1889, English
    [Refereed]
    Scientific journal

  • Shohei Takuno, Ryo Fujimoto, Tetsu Sugimura, Keiichi Sato, Shunsuke Okamoto, Shao-Ling Zhang, Takeshi Nishio
    Oct. 2007, GENETICS, 177(2) (2), 949 - 958, English
    [Refereed]
    Scientific journal

  • Y. Hori, R. Fujimoto, Y. Sato, T. Nishio
    Jul. 2007, THEORETICAL AND APPLIED GENETICS, 115(2) (2), 217 - 224, English
    [Refereed]
    Scientific journal

  • Shunsuke Okamoto, Masashi Odashima, Ryo Fujimoto, Yutaka Sato, Hiroyasu Kitashiba, Takeshi Nishio
    May 2007, PLANT JOURNAL, 50(3) (3), 391 - 400, English
    [Refereed]
    Scientific journal

  • Ryo Fujimoto, Takeshi Nishio
    2007, ADVANCES IN BOTANICAL RESEARCH: INCORPORATING ADVANCES IN PLANT PATHOLOGY, VOL 45, 45, 139 - 154, English
    [Refereed]
    In book

  • Characterization of DNA methyltransferase genes in Brassica rapa
    Ryo Fujimoto, Taku Sasaki, Takeshi Nishio
    Aug. 2006, GENES & GENETIC SYSTEMS, 81(4) (4), 235 - 242, English
    [Refereed]
    Scientific journal

  • Ryo Fujimoto, Tetsu Sugimura, Eigo Fukai, Takeshi Nishio
    Jul. 2006, PLANT MOLECULAR BIOLOGY, 61(4-5) (4-5), 577 - 587, English
    [Refereed]
    Scientific journal

  • T Shinada, Y Kikuchi, R Fujimoto, S Kishitani
    Apr. 2006, PLANT AND CELL PHYSIOLOGY, 47(4) (4), 549 - 553, English
    [Refereed]
    Scientific journal

  • R Fujimoto, T Sugimura, T Nishio
    Jan. 2006, FEBS LETTERS, 580(2) (2), 425 - 430, English
    [Refereed]
    Scientific journal

  • Ryo Fujimoto, Keiichi Okazaki, Eigo Fukai, Makoto Kusaba, Takeshi Nishio
    2006, Genetics, 173(2) (2), 1157 - 1167, English
    [Refereed]
    Scientific journal

  • Characterization of DNA methyltransferase genes in Brassica rapa
    R Fujimoto, T Sasaki, T Nishio
    2006, PLANT AND CELL PHYSIOLOGY, 47(4) (4), S238 - S238, English
    [Refereed]
    Scientific journal

  • E Fukai, R Fujimoto, T Nishio
    Jun. 2003, MOLECULAR GENETICS AND GENOMICS, 269(3) (3), 361 - 369, English
    [Refereed]
    Scientific journal

  • Commonality of self-recognition specificity of S haplotypes between Brassica oleracea and Brassica rapa
    Y Sato, R Fujimoto, K Toriyama, T Nishio
    Jun. 2003, PLANT MOLECULAR BIOLOGY, 52(3) (3), 617 - 626, English
    [Refereed]
    Scientific journal

  • R. Fujimoto, T. Nishio
    May 2003, THEORETICAL AND APPLIED GENETICS, 106(8) (8), 1433 - 1437, English
    [Refereed]
    Scientific journal

  • Recognition specificity of self-incompatibility maintained after the divergence of Brassica oleracea and Brassica rapa
    R Kimura, K Sato, R Fujimoto, T Nishio
    Jan. 2002, PLANT JOURNAL, 29(2) (2), 215 - 223, English
    [Refereed]
    Scientific journal

■ MISC
  • Unveiling the NB-LRR diversity from cultivars of Brassica rapa vegetables
    清水元樹, 藤本龍
    2024, 育種学研究, 26

  • Brassica rapaの極晩抽性系統の選抜とFLCの春化応答性の調査
    矢野秀汰, 清水元樹, 安田剛志, 藤本龍
    2024, 園芸学研究 別冊, 23(1) (1)

  • Refinement of white rust resistance gene loci in Brassica vegetables
    宮路直実, 宮路直実, AKTER M., 清水元樹, 中馬いづみ, 藤本龍
    2023, 育種学研究, 25

  • Development of a DNA marker for white rust resistance in Brassica vegetables.
    宮路直実, 清水元樹, 鈴鴨千鶴子, 勝又雅彦, 安田(高崎)剛志, 中馬いづみ, 藤本龍
    2021, 園芸学研究 別冊, 20(1) (1)

  • Identification of a resistance gene to white rust in Brassica rapa L.
    宮路直実, 清水元樹, 鈴鴨千鶴子, 勝又雅彦, 安田(高崎)剛志, 中馬いづみ, 藤本龍
    2021, 育種学研究, 23

  • Fusarium resistance and FocBr1 polymorphism in Brassica rapa cultivars
    川又美祐, 佐藤真帆, SHEA D., 清水元樹, 藤本龍, 深井英吾, 岡崎桂一
    2021, 育種学研究, 23

  • Resistance mechanism to white rust (Albugo candida) in Brassica rapa L.
    宮路直実, 清水元樹, 安田(高崎)剛志, 中馬いづみ, 藤本龍
    2021, 日本植物病理学会大会プログラム・講演要旨予稿集, 2021

  • Evaluation of the low temperature requirement for vernalization and growth stage responding to vernalization in Turnip rape (Brassica napus L.)
    北村八祥, 小栗速斗, 小堀純奈, 藤本龍, 板橋悦子
    2021, 園芸学研究 別冊, 20(1) (1)

  • Genetic inheritance of white rust disease resistance and genome structure covering white rust resistance gene in Brassica rapa L.
    宮路直実, 清水元樹, 安田(高崎)剛志, 中馬いづみ, 藤本龍
    2020, 日本植物病理学会大会プログラム・講演要旨予稿集, 2020

  • Elucidation of genetic inheritance of white rust disease resistance and analysis of the genome structure covering white rust resistance gene in Brassica rapa L.
    宮路直実, 清水元樹, 安田(高崎)剛志, 中馬いづみ, 藤本龍
    2020, 育種学研究, 22

  • Fine mapping of QTL for tuberous stem formation of kohlrabi
    佐藤庄人, MOZAMMEL H., SHEA D., 松村涼介, 清水元樹, 藤本龍, 深井英吾, 岡崎桂一
    2020, 育種学研究, 22

  • アブラナ科におけるFLCパラログ間の発現量比について
    西田菜美子, AKTER A, 板橋悦子, 宮路直実, MEHRAJ H, 柿崎智博, 岡崎桂一, 藤本龍
    06 Sep. 2019, 育種学研究, 21, 52, Japanese

  • Brassica rapa L.における萎黄病・根こぶ病抵抗性DNAマーカー選抜系の応用
    宮路直実, MEHRAJ Hasan, 清水元樹, 加治誠, 安田(高崎)剛志, 岡崎桂一, 藤本龍
    2019, 園芸学研究 別冊, 18(1) (1)

  • 萎黄病抵抗性・感受性ハクサイ系統における萎黄病菌感染時の免疫応答
    宮路直実, 清水元樹, 加治誠, 安田剛志, 岡崎桂一, 藤本龍
    2019, 日本植物病理学会大会プログラム・講演要旨予稿集, 2019

  • キャベツにおける葉齢依存的な春化応答性のトランスクリプトーム解析
    板橋悦子, SHEA D, 吹野伸子, 藤本龍, 岡崎桂一, 柿崎智博, 小原隆由
    Sep. 2018, 育種学研究, 20(2) (2), 212, Japanese

  • 結球野菜の開花調節機構に関する研究の現状と今後の展望について
    藤本龍, 高田紗都子, 西田菜美子, 板橋悦子, 安田剛志, 柿崎智博, 宇野雄一
    24 Mar. 2018, 園芸学研究 別冊, 17(1) (1), 131, Japanese

  • キャベツの緑体春化におけるFLOWERING LOCUS Cの役割
    板橋悦子, 柿崎智博, 吹野伸子, 岡崎桂一, 藤本龍, 小原隆由
    24 Mar. 2018, 園芸学研究 別冊, 17(1) (1), 132, Japanese

  • ハクサイ市販品種‘W77’の両親系統間リシークエンスによる片親特異的遺伝子変異の同定
    上園倖輔, SHEA Daniel, 清水元樹, 板橋悦子, 宮路直実, 岡崎桂一, 安田(高崎)剛志, 藤本龍
    2018, 園芸学研究 別冊, 17(1) (1)

  • 萎黄病菌キャベツ分離菌抵抗性遺伝子DNAマーカーで萎黄病菌コマツナ分離菌抵抗性を予想できるか?
    宮路直実, 清水元樹, 加治誠, 安田(高崎)剛志, 岡崎桂一, 藤本龍
    2018, 園芸学研究 別冊, 17(1) (1)

  • ハクサイ市販品種‘W77’の両親系統のリシークエンスによるゲノム構造の系統間比較
    上園倖輔, SHEA Daniel, 清水元樹, 板橋悦子, 譚永, 宮路直実, 岡崎桂一, 安田(高崎)剛志, 藤本龍
    2018, 園芸学研究 別冊, 17(1) (1)

  • ハクサイ初期生育期における子葉および本葉サイズ関連遺伝子の探索
    上園倖輔, SHEA D., 清水元樹, 板橋悦子, HASAN M., 岡崎桂一, 安田(高崎)剛志, 藤本龍
    2018, 育種学研究, 20

  • コールラビ茎肥大性のトランスクリプトーム解析
    HOQUE M., HOQUE M., SHEA D., SHEA D., 佐藤庄人, 松村涼介, DOULLAH M., 清水元樹, 藤本龍, 深井英吾, 岡崎桂一
    2018, 育種学研究, 20

  • Brassica oleracea FLC2領域を移入交雑で導入したBrassica rapaの特性解析
    SHEA D, 戸丸祐貴, 戸丸祐貴, 板橋悦子, 宮崎俊夫, 柿崎智博, 清水元樹, 藤本龍, 深井英吾, 岡崎桂一
    Oct. 2017, 育種学研究, 19(2) (2), 123, Japanese

  • アブラナ科植物の雑種強勢に関する研究
    川辺 隆大, FUJIMOTO RYO
    Oct. 2017, アグリバイオ, 1(11) (11), 43 - 44, Japanese
    [Invited]
    Introduction commerce magazine

  • Brassica rapa L.におけるアブラナ科植物白さび病抵抗性遺伝子の探索と検出
    西本良太, 宮路直実, 藤本龍, 土佐幸雄, 中馬いづみ
    12 Apr. 2017, 日本植物病理学会大会プログラム・講演要旨予稿集, 2017, 64, Japanese

  • トランスクリプトーム解析を用いたハクサイの萎黄病菌感染時における免疫応答調査
    宮路直実, 清水元樹, 高田紗都子, 加治誠, 安田(高崎)剛志, 岡崎桂一, 藤本龍
    2017, 育種学研究, 19

  • シロイヌナズナの早期開花エピ変異体の形質評価
    高田紗都子, 石倉園子, 板橋悦子, 宮路直実, 清水元樹, 安田(高崎)剛志, 藤本龍
    2017, 育種学研究, 19

  • Brassica oleraceaにおける萎黄病抵抗性遺伝子FocBo1の多型解析と選抜マーカー開発
    佐藤真帆, 清水元樹, 藤本龍, 深井英吾, 岡崎桂一
    2017, 育種学研究, 19

  • 萎黄病抵抗性・感受性ハクサイ系統における萎黄病菌感染時転写応答の解明
    宮路直実, 清水元樹, 高田紗都子, 加治誠, 安田(高崎)剛志, 岡崎桂一, 藤本龍
    2017, 園芸学研究 別冊, 16(2) (2)

  • アブラナ科植物白さび病菌Albugo candidaに対する抵抗性遺伝子同定に向けたBrassica rapa抵抗性品種の選抜
    西本良太, 宮路直実, 駒寛子, 藤本龍, 土佐幸雄, 中馬いづみ
    24 Sep. 2016, 育種学研究, 18, 115, Japanese

  • Brassica rapa L.における春化によるFLOWERING LOCUS Cのエピジェネティックな転写制御について
    高田紗都子, 板橋悦子, 宮路直実, 清水元樹, 安田剛志, 藤本龍
    2016, 園芸学研究 別冊, 15(2) (2)

  • ハクサイにおける萎黄病菌感染時のトランスクリプトーム解析による病害反応遺伝子の調査
    宮路直実, 清水元樹, 高田紗都子, 加治誠, 安田(高崎)剛志, 岡崎桂一, 藤本龍
    2016, 園芸学研究 別冊, 15(2) (2)

  • Whole genome bisulfite sequencingによるハクサイのDNAメチル化領域の探索
    福島尚樹, 高橋聡史, 板橋悦子, 清水元樹, 安田剛志, 鈴木穣, 関原明, 藤本龍
    2016, 園芸学研究 別冊, 15(2) (2)

  • ハクサイにおけるメチローム解析
    福島尚樹, 高橋聡史, 板橋悦子, 清水元樹, 安田剛志, 鈴木穣, 関原明, 藤本龍
    2016, 育種学研究, 18

  • 萎黄病菌を接種したときの抵抗性と感受性ハクサイ系統のトランスクリプトームプロファイリング
    宮路直実, 清水元樹, 高田紗都子, 加治誠, 安田剛志, 岡崎桂一, 藤本龍
    2016, 育種学研究, 18

  • For the identification of the molecular mechanism of heterosis by epigenetic approach in Brassica
    Takahiro Kawanabe, Ryo Fujimoto
    Dec. 2015, GENES & GENETIC SYSTEMS, 90(6) (6), 373 - 373, English
    Summary international conference

  • ハクサイ市販一代雑種品種“W77”における雑種強勢に関わるQTLの探索
    ABE HIROSHI, KAWANABE TAKAHIRO, SAEKI NATSUMI, SHIMIZU MOTOKI, KONNO SHUHEI, NAGANO ATSUSHI, HONJO MIE, YASUGI MASAKI, KUDO HIROSHI, HATAKEYAMA KATSUNORI, MATSUMOTO SATOSHI, OKAZAKI KEIICHI, KAJI MAKOTO, FUJIMOTO RYO
    26 Sep. 2014, 育種学研究, 16, 111, Japanese

  • Transcriptional regulation of key gene of vernalization, FLOWERING LOCUS C
    Tomaru Yuki, Osabe Kenji, Okazaki Keiichi, Fujimoto Ryo
    To adjust the timing of flowering, plants can sense the temperature and day length. Vernalization is a phenomenon where flowering is induced by a long period of low temperature, and has an important role in controlling flowering time. In Arabidopsis thaliana, FLOWERING LOCUS C (FLC) acts as a floral repressor, and transcription of FLC is repressed through the increase of tri-methylation of histone H3 Lysine 27 (H3K27me3) by vernalization. This transcriptional repression is caused by POLYCOMB REPRESSIVE COMPLEX 2 (PRC2) that functions to modify the H3K27me3. Two types of long non-coding RNAs (lncRNA) from the intragenic region of FLC, COOLAIR and COLDAIR, are expressed by vernalization. Notably COLDAIR leads to an increase of H3K27me3 with PRC2 interaction. A detailed understanding of the regulatory mechanism of the FLC will provide a good model of other epigenetically regulated genes.植物は温度や日長を認識することで開花するタイミングを調節している。春化は、植物が長期間の低温に遭遇することにより花成が誘導される現象であり、開花期を制御する重要な役割を担っている。シロイヌナズナ（Arabidopsis thaliana）の開花抑制遺伝子であるFLOWEING LOCUS C(FLC) では、春化によって、転写抑制にかかわるヒストンマークであるヒストンH3の27番目のリジン残基のトリメチル化（H3K27me3）を介した転写抑制が生じる。この遺伝子サイレンシングにはH3K27me3をターゲットとするPOLYCOMB RPRESSIVE COMPLEX 2（PRC2）複合体が関与する。これは動植物で保存された転写制御メカニズムである。FLC 遺伝子座内から転写される長鎖非翻訳RNA（long non-coding RNA: lncRNA）であるCOOLAIR とCOLDAIR は、春化によって誘導される。特に、COLDAIR はPRC2と結合することで、FLC 遺伝子座内のH3K27me3レベルの増加をもたらす。FLC の制御機構が詳細に理解されれば、他のエピジェネティックな制御を受ける遺伝子のモデルになるだろう。
    新潟大学農学部, Mar. 2014, 新潟大学農学部研究報告, 66(2) (2), 105 - 110, English

  • Screening of DNA markers suitable for purity test of inbred lines in Brassica rapa
    Kawamura Kazutaka, Saeki Natsumi, Ebe Yusuke, Abe Hirofumi, Shimizu Motoki, Osabe Kenji, Okazaki Keiichi, Kawanabe Takahiro, Fujimoto Ryo
    親候補となる近交系統の作成は、植物の一代雑種(F1)品種の育成過程の最初の段階である。一般的に遺伝的に均一な近交系統の育成には5、6世代以上の自殖が必要であると考えられている。DNAマーカーを利用した近交系の純度検定は信頼性が高いが、Brassica rapa L. では、純度検定に有効なDNAマーカーの報告は少ない。本研究では、F1品種の両親系統間の多型を見分けることができるDNAマーカーを見出すために、321のSSRプライマー対を試した。F2集団では、両親系統のゲノムが分離することから、両親系統の塩基配列の多型を追跡することが可能となる。材料にはハクサイの市販F1品種であるW77のF2個体を用いた。59のDNAマーカーでF2集団の個体間で多型が見られたことから、これらのDNAマーカーは純度検定に有効的であると考えられた。
    新潟大学農学部, Mar. 2014, 新潟大学農学部研究報告, 66(2) (2), 111 - 124, English

  • Differential expression解析を用いたハクサイ萎黄病抵抗性候補遺伝子の同定
    清水元樹, 江部裕介, 川辺隆大, 佐伯なつみ, 加治誠, 岡崎桂一, 藤本龍
    2014, 園芸学研究 別冊, 13(1) (1)

  • ハクサイ市販一代雑種品種W77及びそのF2後代の形質調査
    阿部寛史, 川辺隆大, 野瀬笙子, 佐伯なつみ, 清水元樹, 今野周平, 加治誠, 岡崎桂一, 藤本龍
    2014, 育種学研究, 16

  • Brassica oleraceaにおける近交系の純度検定に最適なDNAマーカーのスクリーニングと汎用性
    川村和隆, 玄田真優, 佐々木一嘉, 清水元樹, 佐伯なつみ, 岡崎桂一, 藤本龍, 川辺隆大
    2014, 育種学研究, 16

  • Brassica oleracea近交系の遺伝的均一性評価およびF1種子純度検定に適したDNAマーカーの探索
    川村和隆, 清水元樹, 佐伯なつみ, 阿部寛史, 江部裕介, 岡崎桂一, 川辺隆大, 藤本龍
    2014, 園芸学研究 別冊, 13(2) (2)

  • DNAマーカーを用いたBrassica rapa近交系の基盤整備
    川村和隆, 吉田実央, 清水元樹, 佐伯なつみ, 阿部寛史, 江部裕介, 岡崎桂一, 藤本龍, 川辺隆大
    2014, 育種学研究, 16

  • アブラナ科萎黄病菌(Fusarium oxysporum)のde novo全ゲノムシークエンス情報を用いた分化型特異的遺伝子領域の探索
    船木武人, 田子明日美, PU Zi-jing, 深井英吾, 佐野義孝, 阿部貴志, 藤晋一, 藤本龍, 清水元樹, 夏目俊, 寺内良平, 岡崎桂一
    2014, 育種学研究, 16

  • 藤本 龍, 白澤 沙知子, 川辺 隆大, 岡本 俊介
    公益社団法人 日本農芸化学会, 01 May 2013, 化学と生物, 51(5) (5), 283 - 285, Japanese

  • Screening of DNA markers suitable for purity test of inbred lines in Brassica oleracea
    Tomita Hiroya, Shimizu Motoki, Kume Chiori, Kawanabe Takahiro, Osabe Kenji, Okazaki Keiichi, Fujimoto Ryo
    近交系統の作成は、植物のF1品種の育成過程の最初の段階である。遺伝的に均一な近交系統の育成には5、6世代以上の自殖が必要であると考えられている。DNAマーカーを利用した近交系の純度検定は信頼性が高いが、B. oleraceaでは、純度検定に有効なDNAマーカーの報告は少ない。本研究では、F1品種の両親系統間の多型を見分けることができるDNA マーカーを見出すために、175のSSRプライマー対を試した。F2集団では、両親系統のゲノムが分離することから、両親系統の塩基配列の多型を追跡することが可能となる。そこで、材料にはキャベツの市販F1品種であるYR錦秋協力152のF2個体を用いた。12のDNAマーカーでF2集団の個体間で多型が見られたことから、これらのマーカーは純度検定に有効的であると考えられた。
    新潟大学農学部, Mar. 2013, 新潟大学農学部研究報告 = Bulletin of the Faculty of Agriculture, Niigata University, 65(2) (2), 137 - 147, English

  • ハクサイF1品種W39における生育初期及び収穫期の雑種強勢の評価
    佐伯なつみ, 川辺隆大, 阿部寛史, 今野周平, 清水元樹, 加治誠, 藤本龍
    2013, 園芸学研究 別冊, 12(2) (2)

  • ハクサイF1品種「W77」における生育初期及び収穫期の雑種強勢の評価
    川辺隆大, 阿部寛史, 佐伯なつみ, 清水元樹, 今野周平, 加治誠, 藤本龍
    2013, 園芸学研究 別冊, 12(2) (2)

  • Brassica oleracea染色体添加型B.rapaを用いた緑植物体春化の遺伝解析
    戸丸祐貴, 中村友理, 清水元樹, 藤本龍, 岡崎桂一
    2013, 育種学研究, 15

  • Brassica oleracea品種における萎黄病抵抗性遺伝子FocBo1連鎖DNAマーカーと抵抗性との関連性
    清水元樹, 川辺隆大, PU Zi-Jing, 藤本龍, 岡崎桂一
    2013, 育種学研究, 15

  • Analysis of the promoter activity of anther-specific genes in Arabidopsis thaliana
    Kawanabe Takahiro, Osabe Kenji, Fujimoto ryo
    葯特異的遺伝子やそれらのプロモーター配列の解析は、葯発達に関わる遺伝子の同定や、遺伝子組換えによる雄性不稔植物の作出を目的として、これまで多くなされてきている。本研究では、6つのプロモーター配列の単離を行い、GUS遺伝子に連結してシロイヌナズナに導入することで、葯の発達段階における発現誘導様式を調べた。プロモーターGUSの発現は、様々な花粉発達ステージにおいて観察された。また、塩基配列の解析では葯/花粉特異的遺伝子の発現に必要とされるシス配列が多く含まれていたことから、これらのシス配列が葯特異的に発現誘導していることが示唆された。
    新潟大学農学部, Sep. 2012, 新潟大学農学部研究報告 = Bulletin of the Faculty of Agriculture, Niigata University, 65(1) (1), 31 - 37, English

  • 植物の細胞質雄性不稔性と核遺伝子による稔性の制御について
    板橋 悦子, 川辺 隆大, 藤本 龍
    細胞質雄性不稔性(Cytoplasmic male sterility:CMS)は、ミトコンドリアゲノム上に存在する特有のorfによって引き起こされ、母性遺伝する形質である。多くの場合、核ゲノムにコードされる稔性回復遺伝子(Restorer of fertility:Rf)の存在下で、稔性が回復する。CMSとCMSの維持系統、Rf遺伝子を保持する稔性回復系統を組合せて利用する事で一代雑種育種を行う事ができるため、CMSやその稔性回復は農業上重要な現象である。これまでに同定されているRf遺伝子の多くは、ミトコンドリアに局在するpentatricopeptide repeat(PPR)タンパク質をコードしており、CMS関連ミトコンドリア遺伝子の発現を抑制している。
    新潟大学農学部, Mar. 2012, 新潟大学農学部研究報告 = Bulletin of the Faculty of Agriculture, Niigata University, 64(2) (2), 135 - 142, Japanese

  • 白菜のSハプロタイプ間における自家不和合性の強度の比較
    川辺 隆大, 長部 謙二, 藤本 龍
    アブラナ科野菜の自家不和合性の強度は、F1種子の生産において、自殖種子の混入を避けるために重要な要素である。アブラナ科植物の自家不和合性は、1つの複合遺伝子座(S複合座)によって胞子体型に制御されており、Sハプロタイプ間で優劣性が見られる。しかし、自家不和合性の強度と優劣性との関連性はあまり研究されていない。本研究では、Brassica rapa(白菜)のF1品種に由来するSホモ個体を作成し、3つの独立なSハプロタイプの組合せについて自家不和合性の強度を比較した。優劣性の有無にかかわらず、2つのclass IのSハプロタイプ間では自家不和合性の強度に違いが見られなかった。しかし、花粉において優劣性があるclass Iとclass IIのSハプロタイプ間では、class II Sハプロタイプの自家不和合性の強度はclass I Sハプロタイプに比べて弱かった。これらの結果から、Sハプロタイプ間における自家不和合性の強度は優劣性とは独立していることが示唆された。
    新潟大学農学部, Mar. 2012, 新潟大学農学部研究報告 = Bulletin of the Faculty of Agriculture, Niigata University, 64(2) (2), 143 - 149, English

  • Brassica oleracea根こぶ病抵抗性遺伝子の集積による抵抗性育種
    富田寛也, 清水元樹, 藤本龍, DOULLAH M. A. U., 松元哲, 岡崎桂一
    2012, 育種学研究, 14

  • キャベツ萎黄病抵抗性遺伝子Foc-Bo1の同定とファインマッピング
    清水元樹, PU Zi-Jing, PU Zi-Jing, 富田寛也, 藤本龍, 松元哲, 林武司, ZHANG Y., 岡崎桂一
    2012, 育種学研究, 14

  • RNA-sequence法を用いたハクサイ品種間(S11×R09)特異的なNBS-LRRモチーフ遺伝子の探索
    清水元樹, 清水元樹, 江部裕介, 藤本龍, 川邊隆大, YING Hua, 加治誠, DENNIS Elizabeth, 岡崎桂一
    2012, 育種学研究, 14

  • Analysis of Organ-specific Regulation of DNA Methylation in Brassica rapa by MSAP
    Sasaki Taku, Kawanabe Takahiro, Fujimoto Ryo
    DNA methylation is an important epigenetic modification regulating gene expression. In this study, we investigateddifferences of DNA methylation status between leaves, stamens, and pistils by methylation sensitive amplificationpolymorphism (MSAP) analysis in Brassica rapa. The difference of DNA methylation status was the largest between leavesand stamens/pistils. About half of the differentially methylated sequences were genic regions, but their expression levelsdid not differ between organs. The percentage of genic regions detected by MSAP analysis was higher than that of the totallength of the genic regions in the genome, suggesting that genic regions are differentially methylated between organs in B.rapa.DNA のメチル化は遺伝子の転写制御に重要なエピジェネティックな変化である。本研究では、Brassica rapa の葉、雄しべ、雌しべのDNA のメチル化レベルの違いについてMSAP 法を用いて調べた。DNA のメチル化は葉と雄しべ/ 雌しべで最も違っていた。DNA のメチル化が異なっていた配列の半分は遺伝子領域であったが、遺伝子の発現量は3つの器官で違いが見られなかった。MSAP 法で見出された領域の内、遺伝子領域が占める割合は、ゲノム中に占める遺伝子領域の割合よりも高かったことから、B. rapa において、器官特異的なDNA のメチル化の違いは遺伝子領域に起こりやすい可能性が示された。
    新潟大学農学部, Sep. 2011, 新潟大学農学部研究報告, 64(1) (1), 7 - 16, English

  • Dominant/Recessive Relationship in Male Determinant Gene of Self-incompatibility in Brassica
    Fujimoto Ryo, Kawanabe Takahiro, Sasaki Taku, Okazaki Keiichi
    アブラナ科植物の自家不和合性は、S複合座に座乗するSRK（S receptor kinase）とSP11／SCR（S locus protein 11／S locus cysteine rich）のS特異的な相互作用により決定されている。アブラナ科植物の自家不和合性は、胞子体型自家不和合性であることから、花粉の表現型は親の遺伝子型によって決定される。SP11／SCRの対立遺伝子間で優劣性が見られる場合、ヘテロ接合体の花粉では、劣性側のSP11／SCRの発現が抑制される。この劣性側のSP11／SCRの転写抑制は、優勢側のS複合座から、劣性側のSP11／SCRのプロモーター領域と相同性があるsmall RNAが発現し、その結果、劣性側のSP11／SCRのプロモーター領域にDNAのメチル化が誘導されることで起こる可能性が示唆された。
    新潟大学農学部, Sep. 2011, 新潟大学農学部研究報告, 64(1) (1), 17 - 26, Japanese

  • エピジェネティクスが制御する植物の形質
    藤本 龍, ISHIKAWA RYO
    遺伝子の発現情報の伝達は塩基配列に依存するジェネティックな遺伝と塩基配列によらず、遺伝子の修飾状態の変化に依存するエピジェネティックな遺伝が存在する．植物のエピジェネティクス研究は、モデル植物であるシロイヌナズナで進んでおり、特に突然変異体を用いた解析がこの分野の進展に大きく貢献している．動物のエピジェネティックな制御に関連する遺伝子の突然変異体は、致死に到ることが多いが、植物の突然変異体では致死に到らず、遺伝学的な解析が可能であることが理由として挙げられる．また、エピジェネティックな遺伝子の発現制御は、遺伝子機能・環境応答・生殖制御などとの関連性が示唆されていることからも育種において重要な研究課題である．本総説では、育種学的観点からエピジェネティックな制御機構の重要性について、トランスポゾン・生殖・環境応答の項目に分けて概説し、またエピジェネティックな現象による重要農業形質の例について最近の話題を交えて紹介する．
    日本育種学会, 2010, 育種学研究, 12(4) (4), 123 - 131, Japanese
    [Refereed]
    Introduction scientific journal

  • Recent molecular biological studies on Brassica self-incompatibility and future perspectives
    Fujimoto R, Nishio T
    アブラナ科植物の多くは、近親交配を防ぐために、自己花粉の発芽・柱頭内への花粉管の侵入を特異的に阻害する機構である自家不和合性を有している。アブラナ科植物の自家不和合性は、基本的には多数の複対立遺伝子を持つ1遺伝子によって説明され、花粉と雌しべのS対立遺伝子が一致したときに、柱頭内への花粉管の侵入が阻害される。また、花粉の認識特異性が胞子体の遺伝子型によって決定されるため、胞子体型自家不和合性に分類される。自己花粉が認識されて花粉管の侵入が柱頭上で抑制されることから、柱頭と花粉に自家不和合性の自他認識反応に関与する分子が存在すると考えられた。アブラナ科植物には、主要な野菜であるキャベツ、ブロッコリー、カリフラワーやカブ、ハクサイ、ツケナ類、チンゲンサイ及び、ダイコンが含まれ、自家不和合性を利用した一代雑種品種の採種が商業的に行われている。アブラナ科植物の自家不和合性は、分子生物学的に興味深い現象であると同時に、育種学的にも重要な研究課題である。
    Japanese Society of Breeding, Mar. 2008, Breeding Research, 10(1) (1), 1 - 9, Japanese
    Introduction other

  • Evolution of imprinted FWA gene in Arabidopsis
    Fujimoto Ryo, Kawabe Akira, Kinoshita Yuki, Takashima Kazuya, Kinoshita Tetsu, Miura Asuka, Kakutani Tetsuji
    Dec. 2007, GENES & GENETIC SYSTEMS, 82(6) (6), 549 - 549, English
    Summary international conference

  • Analysis of genome structure of class II self-incompatibility (s) locus in Brassica rapa
    FUKAI E., FUJIMOTO R., NISHIO T.
    26 Aug. 2002, 育種学研究 = Breeding research, 4, 346 - 346, Japanese

  • Comparison of genome structure of class I self-incompatibility (S) locus between Brassica oleracea and Brassica rapa
    FUJIMOTO R., NISHIO T.
    26 Aug. 2002, 育種学研究 = Breeding research, 4, 347 - 347, Japanese

  • Identification of S haplotype in Brassica by dot blot analysis of SP11 alleles
    FUJIMOTO R., NISHIO T.
    30 Mar. 2002, 育種学研究 = Breeding research, 4(1) (1), 256 - 256, Japanese

■ Books And Other Publications
  • The role of epigenetic transcriptional regulation in Brassica vegetables: a potential resource for epigenetic breeding
    Yoshiki Kamiya, Saaya Shiraki, Kazumasa Fujiwara, Mst. Arjina Akter, Ayasha Akter, Ryo Fujimoto, Hasan Mehraj
    Contributor, Chapter 1, Springer Singapore, Jan. 2023, ISBN: 9789811953668

  • The current status and perspective of research on hybrid vigor in plants
    Kazumasa Fujiwara, Saya Shiraki, Yoshiki Kamiya, Ryo Fujimoto
    Joint work, 第96巻・第12号 1075-1085, YOKENDO, Dec. 2021

  • Chinese Cabbage (Brassica rapa L. var. pekinensis) Breeding: Application of Molecular Technology
    Takumi Okamoto, Xiaochun Wei, Hasan Mehraj, Mohammad Rashed Hossain, Ayasha Akter, Naomi Miyaji, Yoshinobu Takada, Jong-In Park, Ryo Fujimoto, Ill-Sup Nou, Masao Watanabe
    Joint work, pp 59-94, Advances in Plant Breeding Strategies: Vegetable Crops. Springer, Cham, Aug. 2021

  • Breeding for Disease Resistance in Brassica Vegetables Using DNA Marker Selection
    Mst Arjina Akter, Hasan Mehraj, Takeru Itabashi, Tomoe Shindo, Masaaki Osaka, Ayasha Akter, Naomi Miyaji, Naoki Chiba, Junji Miyazaki, Ryo Fujimoto
    Joint work, IntechOpen, Feb. 2021

  • The Importance of Genetic and Epigenetic Research in the Brassica Vegetables in the Face of Climate Change
    Honghao Lv, Naomi Miyaji, Kenji Osabe, Ayasha Akter, Hasan Mehraj, Daniel J. Shea and Ryo Fujimoto
    Joint work, 161-256, Springer, Mar. 2020

  • Chapter 8 Hybrid Vigor: Importance of Epigenetic Processes and Consequences for Breeding
    Naomi Miyaji, FUJIMOTO RYO
    Joint work, Advances in Botanical Research-Plant Epigenetics Coming of Age for Breeding Applications, Nov. 2018, English
    Scholarly book

  • Genetic and Epigenetic Regulation of Vernalization in Brassicaceae
    Ayasha Akter, Namiko Nishida, Satoko Takada, Etsuko Itabashi, Kenji Osabe, Daniel J Shea, FUJIMOTO RYO
    Joint work, IntechOpen, Oct. 2018, English
    Scholarly book

  • 遺伝学の基礎 14章 エピジェネティクス
    FUJIMOTO RYO
    Others, 朝倉書店, Sep. 2018, Japanese
    Textbook

  • 育種学研究
    貴島 祐治, 寺内 良平, 高田 美信, 宅見 薫雄, FUJIMOTO RYO, 村井 耕二
    Joint work, 日本育種学会, Jul. 2014, Japanese
    Scholarly book

  • 植物の雑種強勢の分子生物学的な研究と展望
    FUJIMOTO RYOU, 白澤 沙知子, 川辺 隆大, 岡本 俊介
    Joint work, 日本農芸化学会, May 2013, Japanese
    Scholarly book

  • Self‐Incompatibility
    FUJIMOTO RYO, Takeshi Nishio
    Joint work, Advances in Botanical Research, 2007, English
    Scholarly book

■ Lectures, oral presentations, etc.
  • Identification of Factors That Regulate Early Flowering in ddm1 Mutant of C24 Accession in Arabidopsis thaliana
    Kohei Kunita, Motoki Shimizu, Ryo Fujimoto
    Plant and Animal Genome Conference (PAG32), Jan. 2025, English
    Poster presentation

  • Common Mechanisms Between the Decrease in Heterosis Levels By Loss of DDM1 Function and the Reduction in Biomass By Shade Avoidance Syndrome
    Keita Nishimura, Kodai Matsuo, Motoki Shimizu, Ryo Fujimoto
    Plant and Animal Genome Conference (PAG32), Jan. 2025, English
    Poster presentation

  • DNA マーカー選抜による複合病害抵抗性育種
    藤本龍
    令和6年度野菜花き課題別研究会-アブラナ科野菜の育種・生産を取り巻く現状と展望, Oct. 2024, Japanese
    [Invited]
    Invited oral presentation

  • Genetic and epigenetic approaches to hybrid vigor, vernalization, ad disease responsiveness in Brassicaceae
    Ryo Fujimoto
    Special Seminar-University of Nebraska–Lincoln, Sep. 2024, English
    Public discourse

  • Brassica rapa野菜 NB-LRR遺伝子の掌握
    清水 元樹, 藤本 龍
    日本育種学会 第146回講演会, Sep. 2024, Japanese
    Poster presentation

  • シロイヌナズナC24 系統のddm1変異体を早期開花させる因子の探索
    國田 康平, 藤本 龍
    日本育種学会 第146回講演会, Sep. 2024, Japanese
    Oral presentation

  • 老化関連遺伝子が DDM1の機能喪失による雑種強勢低減と避陰反応による葉面積減少に関与する可能性について
    西村 慶太, 藤本 龍
    日本育種学会第146回講演会, Sep. 2024, Japanese
    Oral presentation

  • ハクサイにおける避陰反応による生育低下を引き起こす原因遺伝子の探索
    西村慶太, Hasan Mehraj, 安田剛志, 藤本龍
    令和６年度園芸学会近畿支部滋賀大会, Aug. 2024, Japanese
    Oral presentation

  • Brassica rapaにおける根こぶ病菌感染により発現が変動する遺伝子の同定
    藤本龍, 清水元樹, 鎌田諒徳, 鈴鴨千鶴子, 加治 誠, 勝又雅彦, 中馬いづみ
    令和６年度園芸学会近畿支部滋賀大会, Aug. 2024, Japanese
    Poster presentation

  • Functional assessment and epigenome editing of Brassica rapa FLC in Arabidopsis thaliana
    Kenji Osabe, Miharu Yamada, Akhi Paul Chowdhury, Ryo Fujimoto, Diana M Buzas
    The 13th International Congress on Plant Molecular Biology, Jun. 2024, English
    Poster presentation

  • Are additive histone modifications important for exhibiting heterosis in Chinese cabbage?
    Hasan Mehraj, Satoshi Takahashi, Motoaki Seki, Ryo Fujimoto
    The 13th International Congress on Plant Molecular Biology, Jun. 2024, English
    Poster presentation

  • IDENTIFICATION OF GENES WHOSE EXPRESSION FLUCTUATES FOLLOWING PATHOGEN INFECTION IN BRASSICA RAPA VEGETABLES
    Ryo Fujimoto, Naomi Miyaji, Mst Arjina Akter, Motoki Shimizu, Hasan Mehraj, Md Asad-Ud Doullah, Izumi Chuma
    International Conference on Advanced Agricultural Research, May 2024, English
    [Invited]
    Invited oral presentation

  • ナデシコ属植物の春化応答に関わるFLCオルソログの解析
    八木雅史, 小西晴香, 小野凌汰, 藤本龍, 岡本花弥, 小山竜平, 宇野雄一
    園芸学会令和6年度春季大会, Mar. 2024
    Poster presentation

  • Brassica rapaの晩抽性分子育種への試み
    藤本龍, 矢野秀汰, 國田康平, チョウドリー アキ, 安田剛志
    園芸学会令和6年度春季大会, Mar. 2024
    Oral presentation

  • シロイヌナズナのddm1変異体の系統間比較
    西村慶太, 國田康平, 神矢良樹, 藤本龍
    日本育種学会 第145回講演会, Mar. 2024, Japanese
    Oral presentation

  • Comparison of the transcriptional response of FLC following vernalization in two different growth stages in artificially synthesized Brassica napus
    Ryo Fujimoto, Motoki Shimizu, Elizabeth Dennis, Keiichi Okazaki
    The 16th International Rapeseed Congress (IRC) 2023, Sep. 2023, English
    Poster presentation

  • Loss of DDM1 function leads to reduced levels of heterosis in Arabidopsis thaliana
    Keita Nishimura, Yoshiki Kamiya, Kodai Matsuo, Motoki Shimizu, Elizabeth S. Dennis, Ryo Fujimoto
    International Plant and Animal Genome Conference (PAG Australia), Sep. 2023, English
    Poster presentation

  • Characterization of FLOWERING LOCUS C 5 in Brassica rapa L.
    Kohei Kunita, Ayasha Akter, Tomohiro Kakizaki, Etsuko Itabashi, Motoki Shimizu, Mst. Arjina Akter, Hasan Mehraj, Keiichi Okazaki, Elizabeth S Dennis, Ryo Fujimoto
    International Plant and Animal Genome Conference (PAG Australia), Sep. 2023, English
    Poster presentation

  • Idenfification of the genetic region related to heterosis in Arabidopsis thaliana
    Kazumasa Fujiwara, Naomi Miyaji, Motoki Shimizu, Ryo Fujimoto
    International Plant and Animal Genome Conference (PAG Australia), Sep. 2023, English
    Poster presentation

  • Characterization of the transcriptional immune response to Albugo candida in Brassica rapa L.
    Mst Arjina Akter, Naomi Miyaji, Motoki Shimizu, Elizabeth S. Dennis, Izumi Chuma, Ryo Fujimoto
    International Plant and Animal Genome Conference (PAG Australia), Sep. 2023, English
    Poster presentation

  • Overexpression of ASC8 affects growth and heterosis in Arabidopsis thaliana
    Saaya Shiraki, Takashi Yasuda, Ryo Fujimoto
    International Plant and Animal Genome Conference (PAG Australia), Sep. 2023, English
    Poster presentation

  • The Association between Salt Tolerance and Enhancement of Biomass in Arabidopsis thaliana Hybrids
    Yoshiki Kamiya, Hasan Mehraj, Takeshi Takasaki-Yasuda, Ryo Fujimoto
    International Plant and Animal Genome Conference (PAG Australia), Sep. 2023, English
    Poster presentation

  • コマツナにおける白さび病菌感染による転写応答の系統間比較
    アクタ アルジーナ, 宮路 直実, 清水 元樹, 中馬 いづみ, 藤本 龍
    日本育種学会 第144回講演会, Sep. 2023, Japanese
    Poster presentation

  • シロイヌナズナの耐塩性とバイオマスのヘテロシ スの関係性
    神矢 良樹, Mehraj Hasan, 安田 剛志, 藤本 龍
    日本育種学会 第144回講演会, Sep. 2023, Japanese
    Oral presentation

  • シロイヌナズナの雑種強勢に関わるゲノム領域の探求
    藤原 一将, 宮路 直実, 安田 剛志, 藤本 龍
    日本育種学会 第144回講演会, Sep. 2023, Japanese
    Oral presentation

  • DDM1の機能喪失による雑種強勢低減と避陰反応による生育低下に共通する遺伝子の探索
    西村 慶太, 松尾 康大, 安田 剛志, 藤本 龍
    日本育種学会 第144回講演会, Sep. 2023, Japanese
    Oral presentation

  • アブラナ科野菜における白さび病抵抗性遺伝子座 乗領域の詳細化
    宮路 直実, アクター アルジーナ, 清水 元樹, 中馬 いづみ, 藤本 龍
    日本育種学会 第144回講演会, Sep. 2023, Japanese
    Oral presentation

  • シロイヌナズナにおける ACS8 の発現上昇が雑種 強勢に及ぼす影響
    白木 沙彩, 松尾 康大, 安田 剛志, 藤本 龍
    日本育種学会 第144回講演会, Sep. 2023, Japanese
    Oral presentation

  • Brassica rapaの極晩抽性系統の選抜とFLCの春化応答性の調査
    矢野秀汰, 清水元樹, 安田剛志, 藤本龍
    令和５年度 園芸学会近畿支部兵庫大会, Sep. 2023, Japanese
    Oral presentation

  • Brassica rapaにおけるFLOWERING LOCUS C 5の評価
    國田康平, 藤本龍
    令和５年度 園芸学会近畿支部兵庫大会, Sep. 2023, Japanese
    Oral presentation

  • Brassica rapaエピジェネティック変異体におけるDNAメチル化レベルの低下と生育への影響
    神矢良樹, 奥崎文子, 殿崎薫, 肥塚信也, 藤本龍
    令和５年度 園芸学会近畿支部兵庫大会, Sep. 2023, Japanese
    Oral presentation

  • Role of FLOWERING LOCUS C 5 as a floral repressor in Brassica rapa L.
    Kohei Kunita, Ayasha Akter, Tomohiro Kakizaki, Motoki Shimizu, Keiichi Okazaki, Elizabeth S Dennis, Ryo Fujimoto
    The 4th Asian Horticultural Congress (AHC2023), Aug. 2023, English
    Poster presentation

  • Comparison of the histone modification H3 lysine 27 tri-methylation states between accessions in Chinese cabbage
    Saaya Shiraki, Hasan Mehraj, Satoshi Takahashi, Motoaki Seki, Elizabeth S Dennis, Ryo Fujimoto
    The 4th Asian Horticultural Congress (AHC2023), Aug. 2023, English
    Poster presentation

  • Typing and distribution of the SFBB alleles encoding in four S-haplotypes of European pear (Pyrus communis L.)
    Kohta Tadokoro, Matsuri Kobayashi, Ryo Fujimoto, Takeshi Takasaki-Yasuda
    The 4th Asian Horticultural Congress (AHC2023), Aug. 2023, English
    Poster presentation

  • Identification of canididate genes associated with the defense response following Albugo candida inoculation in Brassica rapa L.
    Mst Arjina Akter, Naomi Miyaji, Motoki Shimizu, Hasan Mehraj, Md Asad-Ud Doullah, Elizabeth S. Dennis, Izumi Chuma, Ryo Fujimoto
    The 4th Asian Horticultural Congress (AHC2023), Aug. 2023, English
    Oral presentation

  • Genetic and epigenetic approaches for understanding molecular mechanism of heterosis in shoots at early developmental stages of Chinese cabbage
    Ryo Fujimoto, Hasan Mehraj, Satoshi Takahashi, Motoki Shimizu, Takeshi Yasuda, Motoaki Seki, Elizabeth S. Dennis
    The 4th Asian Horticultural Congress (AHC2023), Aug. 2023, English
    Oral presentation

  • New Chinese cabbage cultivar 'Saiten Neo 70' reduces the risk of clubroot disease
    Keita Nishimura, Tomoe Shindo, Takeru Itabashi, Naoki Chiba, Hiroshi Kano, Akitoshi Sawasato, Hayato Takahashi, Shun Ito, Makoto Kaji, Masahiko Katsumata, Ryo Fujimoto
    The 4th Asian Horticultural Congress (AHC2023), Aug. 2023, English
    Poster presentation

  • Albugo candida inoculation has different effects on DNA methylation in resistant and susceptible accessions of komatsuna
    Yoshiki Kamiya, Mst Arjina Akter, Soodeh Tiranz, Naomi Miyaji, Shohei Takuno, Phillipp E. Bayer, Motoki Shiimzu, David Edwards, Jacqueline Batley, Ryo Fujimoto
    The 4th Asian Horticultural Congress (AHC2023), Aug. 2023, English
    Poster presentation

  • Investigation of heterosis in the salinity tolerance of intraspecific hybrid in Arabidopsis thaliana
    Yoshiki Kamiya, Hasan Mehraj, Ryo Fujimoto
    The 33rd International Conference on Arabidopsis Research (ICAR2023), Jun. 2023, English
    Poster presentation

  • High parent dominance of trimethylation of lysine 27 of histone H3 level in intraspecific hybrids of Arabidopsis thaliana
    Saaya Shiraki, Kodai Matsuo, Satoshi Takahashi, Motoki Shimizu, Elizabeth S Dennis, Motoaki Seki, Ryo Fujimoto
    The 33rd International Conference on Arabidopsis Research (ICAR2023), Jun. 2023, English
    Poster presentation

  • For identification of factors on early flowering in ddm1 mutant of C24 accession in Arabidopsis thaliana
    KOHEI KUNITA, Motoki Shimizu, Ryo Fujimoto
    The 33rd International Conference on Arabidopsis Research (ICAR2023), Jun. 2023, English
    Poster presentation

  • The Influence of loss of DECREASE IN DNA METHYLATION 1 function on heterosis in Arabidopsis thaliana
    Keita Nishimura, Yoshiki Kamiya, Kodai Matsuo, Motoki Shimizu, Elizabeth S. Dennis, Ryo Fujimoto
    The 33rd International Conference on Arabidopsis Research (ICAR2023), Jun. 2023, English
    Poster presentation

  • Brassica rapaにおけるH3K4me3とH3K36me3の遺伝子発現における役割
    白木沙彩, Mehraj Hasan, 安田剛志, 藤本龍
    園芸学会令和5年度春季大会, Mar. 2023, Japanese
    Oral presentation

  • Induction of salicylic acid-responsive genes triggers disease resistance of Brassica rapa L.
    Akter Mst. Arjina, 宮路直実, 藤本龍
    園芸学会令和5年度春季大会, Mar. 2023, English
    Oral presentation

  • コールラビの塊茎肥大に関連するコールラビとブロッコリーの比較トランスクリプトームと植物ホルモンプロファイリング
    Md Nuruzzaman, 小嶋美紀子, 佐藤庄人, 竹林裕美子, Mozammel Hoque, 岡本暁, シェイ ダニエル ジョン, 清水元樹, 藤本龍, 榊原均, 深井英吾, 岡崎桂一
    日本育種学会第143回講演会, Mar. 2023, English
    Oral presentation

  • アブラナ科野菜の晩抽性育種においてBrFLC5は考慮しなくていいか?
    藤本龍, 國田康平, 柿崎智博, 板橋悦子, 岡崎桂一
    日本育種学会第143回講演会, Mar. 2023, Japanese
    Oral presentation

  • シロイヌナズナの早期開花変異体の原因遺伝子同定に向けて
    藤本龍, 國田康平, ブザス ディアナ
    筑波大学形質転換植物デザイン研究拠点令和4年度成果報告会, Mar. 2023, Japanese
    Oral presentation

  • Genetic and epigenetic approaches for understanding the molecular mechanism of heterosis in Arabidopsis thaliana and Brassica rapa
    Ryo Fujimoto, Hasan Mehraj, Satoshi Takahashi, Kazumasa Fujiwara, Naomi Miyaji, Saaya Shiraki, Motoki Shimizu, Motoaki Seki, Elizabeth S. Dennis
    Cold Spring Harbor Asia conference on Integrative Epigenetics in Plants, Dec. 2022, English
    Oral presentation

  • Change of trimethylation of lysine 27 of histone H3 in the decrease in DNA methylation 1 mutant of Arabidopsis thaliana
    Saaya Shiraki, Kodai Matsuo, Satoshi Takahashi, Motoki Shimizu, Elizabeth S. Dennis, Motoaki Seki, Ryo Fujimoto
    Cold Spring Harbor Asia conference on Integrative Epigenetics in Plants, Dec. 2022, English
    Poster presentation

  • The relationship between the function of DECREASE IN DNA METHYLATION 1 (DDM1) and the salinity tolerance of Arabidopsis thaliana accessions, Columbia-0 and C24
    Yoshiki Kamiya, Hasan Mehraj, Kodai Matsuo, Motoki Shimizu, Satoshi Takahashi, Motoaki Seki, Takeshi Takasaki, Ryo Fujimoto
    Cold Spring Harbor Asia conference on Integrative Epigenetics in Plants, Dec. 2022, English
    Poster presentation

  • Identification of the genetic region related to heterosis in Arabidopsis thaliana
    Kazumasa Fujiwara, Naomi Miyaji, Satoshi Takahashi, Motoki Shimizu, Motoaki Seki, Takeshi Yasuda, Elizabeth S. Dennis, Ryo Fujimoto
    Cold Spring Harbor Asia conference on Integrative Epigenetics in Plants, Dec. 2022, English
    Poster presentation

  • Role of four major histone midifications in regulating heterosis in Chinease cabbage
    Hasan Mehraj, Satoshi Takahashim, Motoki Shimizu, Takeshi Yasuda, Elizabeth S. Dennis, Motoaki Seki, Ryo Fujimoto
    Cold Spring Harbor Asia conference on Integrative Epigenetics in Plants, Dec. 2022, English
    Poster presentation

  • The role of DNA methylation in disease resitance against Albugo candida inoculation in Brassica rapa var. perviridis
    Mst. Arjina Akter, Soodeh Tirnaz, Naomi Miyaji, Shohei Takuno, Philipp E. Bayer, Motoki Shimizu, David Edwards, Jacqueline Batley, Ryo Fujimoto
    Cold Spring Harbor Asia conference on Integrative Epigenetics in Plants, Dec. 2022, English
    Poster presentation

  • シロイヌナズナの雑種強勢に関係する遺伝領域の同定
    藤原一将, 宮路直実, 安田剛志, 藤本龍
    日本育種学会第142回講演会, Sep. 2022, Japanese
    Oral presentation

  • シロイヌナズナの耐塩性における雑種強勢の評価
    神矢良樹, Mehraj Hasan, 安田剛志, 藤本龍
    日本育種学会第142回講演会, Sep. 2022, Japanese
    Oral presentation

  • ハクサイにおける長鎖非コードRNAゲノム領域のエピジェネティック修飾
    神矢良樹, Mehraj Hasan、Akiter, Mst. Arjina, 高橋聡史, 関原明, 安田剛志, 藤本龍
    園芸学会令和4年度秋季大会, Sep. 2022, Japanese
    Poster presentation

  • ハクサイにおいて活性型ヒストン修飾が遺伝子発現に及ぼす影響
    白木沙彩, Mehraj Hasan, 高橋聡史, 安田剛志, 関原明, 藤本龍
    園芸学会令和4年度秋季大会, Sep. 2022, Japanese
    Poster presentation

  • The role of histone modification in gene expression in Brassica rapa vegetables
    Saaya Shiraki, Hasan Mehraj, Satoshi Takahashi, Ayasha Akter, Naomi Miyaji, Motoaki Seki, Elizabeth S. Dennis, Ryo Fujimoto
    The 31st International Horticultural Congress (IHC2022), Aug. 2022, English
    Oral presentation

  • The role of salicylic acid responsive genes in disease resistance in Brassica rapa vegetables
    Naomi Miyaji, Mst. Arjina Akter, Motoki Shimizu, Takeshi Takasaki-Yasuda, Ryo Fujimoto
    The 31st International Horticultural Congress (IHC2022), Aug. 2022, English
    Oral presentation

  • The role of epigenetic modifications in the transcriptional regulation of long noncoding RNAs in Brassica rapa vegetables
    Yoshiki Kamiya, Hasan Mehraj, Satoshi Takahashi, Ayasha Akter, Naomi Miyaji, Motoaki Seki, Elizabeth S. Dennis, Kenji Osabe, Ryo Fujimoto
    The 31st International Horticultural Congress (IHC2022), Aug. 2022, English
    Oral presentation

  • The epigenome analysis in Brassica rapa L.
    Ryo Fujimoto
    School of Life and Medical Sciences Research Conference, Jun. 2022, English
    [Invited]
    Invited oral presentation

  • Brassica rapaにおける白さび病菌の感染がDNAメチル化に及ぼす影響
    宮路直実, 宅野将平, 藤本 龍
    園芸学会令和4年度春季大会, Mar. 2022, Japanese
    Poster presentation

  • Brassica rapaにおける極晩抽性系統の春化特性評価
    Akter Ayasha, 藤本龍
    日本育種学会第141回講演会, Mar. 2022, Japanese
    Oral presentation

  • ニホンナシS2, S3, S4ハプロタイプのゲノム構造解析とSFBBの探索
    西村遼太郎, 溝口桃香, 張競月, 藤本龍, 安田(高崎)剛志
    園芸学会令和4年度春季大会, Mar. 2022, Japanese
    Oral presentation

  • Prospects for increasing the density of genetic markers in Chinese cabbage linkage map using GRAS-Di technique
    Mehraj Hasan, 安田剛志, 藤本龍
    園芸学会令和4年度春季大会, Mar. 2022, English
    Oral presentation

  • Transcriptional association of mRNAs and their paired natural antisense transcripts following Fusarium oxysporum inoculation in Brassica rapa L.
    Akter Mst. Arjina, Mehraj Hasan, 藤本龍
    園芸学会令和4年度春季大会, Mar. 2022, English
    Oral presentation

  • Brassica rapaの春化応答因子の同定に向けて
    藤本龍, アクタ アヤシャ, ブザス ディアナ
    筑波大学形質転換植物デザイン研究拠点 令和3年度成果報告会, Mar. 2022, Japanese
    Oral presentation

  • Comparative paralog histone modification states following whole genome triplication in Brassica rapa L.
    Hasan Mehraj, Satoshi Takahashi, Naomi Miyaji, Motoaki Seki, Elizabeth S Dennis, Ryo Fujimoto
    2021 Cold Spring Harbor meeting: Plant Genomes, Systems Biology and Engineering (Virtual) ., Dec. 2021, English
    Poster presentation

  • アブラナ科野菜における新規萎黄病抵抗性 DNA マーカーの開発
    宮路直実, 鈴鴨千鶴子, 清水元樹, 進藤友恵, 板橋建, 加治誠, 勝又雅彦, 藤本龍
    園芸学会令和3年度秋季大会, Sep. 2021, Japanese
    Poster presentation

  • Brassica rapa L. における萎黄病抵抗性育種のための共優性 SSR マーカーの開発
    進藤友恵, 大坂正明, 千葉直樹, 勝又雅彦, 鈴鴨千鶴子, 加治誠, 藤本龍, 板橋建
    園芸学会令和3年度秋季大会, Sep. 2021, Japanese
    Poster presentation

  • Characterization of Histone H3 Lysine 4 and 36 Tri-methylation in Brassica rapa L.
    Hasan Mehraj, Satoshi Takahashi, Yutaka Suzuki, Motoaki Seki, Elizabeth S. Dennis, Ryo Fujimoto
    2021 Australian Brassica Conference, Sep. 2021, English
    Oral presentation

  • アブラナ科野菜における白さび病抵抗性DNAマーカーの開発
    宮路直実, 清水元樹, 鈴鴨千鶴子, 勝又雅彦, 安田(高崎)剛志, 中馬いづみ, 藤本龍
    園芸学会令和３年度春季大会, Mar. 2021, Japanese
    Poster presentation

  • アブラナ科野菜におけるサリチル酸応答遺伝子の同定
    宮路直実, 安田剛志, 藤本龍
    園芸学会令和３年度春季大会, Mar. 2021, Japanese
    Poster presentation

  • ナバナ（Brassica napus L.）の春化に必要な低温要求量と春化応答を示す生育ステージの評価
    北村八祥, 小栗速斗, 小堀純奈, 藤本龍, 板橋悦子
    園芸学会令和３年度春季大会, Mar. 2021, Japanese
    Poster presentation

  • Brassica rapa品種の萎黄病抵抗性とFocBr1抵抗性遺伝子の多型解析
    川又美祐, 佐藤真帆, シェイダニエル, 清水元樹, 藤本龍, 深井英吾, 岡崎桂一
    日本育種学会 第139回講演会, Mar. 2021, Japanese

  • Brassica rapa L.における白さび病抵抗性遺伝子の同定
    宮路直実, 清水元樹, 鈴鴨千鶴子, 勝又雅彦, 安田(高崎) 剛志, 中馬いづみ, 藤本龍
    日本育種学会 第139回講演会, Mar. 2021, Japanese
    Oral presentation

  • Brassica rapa L.における白さび病抵抗性機構について
    宮路直実, 清水元樹, 安田(高崎)剛志, 中馬いづみ, 藤本龍
    令和3年度日本植物病理学会大会, Mar. 2021, Japanese
    Oral presentation

  • DNAメチル化転移酵素によるシロイヌナズナの雑種強勢制御機構
    山下萌, 安田(高崎)剛志, 藤本龍
    日本育種学会 第138回講演会, Oct. 2020, Japanese
    Oral presentation

  • シロイヌナズナにおけるヒストン脱アセチル化酵素HDA6が雑種強勢に及ぼす影響
    村上照実, 安田 (高崎) 剛志, 藤本龍
    日本育種学会 第138回講演会, Oct. 2020, Japanese
    Oral presentation

  • キャベツにおける春化応答性の品種間差とBoFLC2遺伝子の関係
    板橋 悦子, シェイ ダニエル, 吹野 伸子, 藤 本 龍, 岡崎 桂一, 柿崎 智博, 小原 隆由
    日本育種学会第137回講演会, Mar. 2020
    Oral presentation

  • Brassica rapa L. における白さび病抵抗性遺伝子の遺伝様式の解明と抵抗性遺伝子候補の探索
    宮路 直実, 清水 元樹, 安田 ( 高崎 ) 剛志, 中 馬 いづみ, 藤本 龍
    日本育種学会第137回講演会, Mar. 2020
    Oral presentation

  • キャベツ BoFLC2 導入ハクサイ系統の春化特性
    西田 菜美子, 岡崎 桂一, 藤本 龍
    日本育種学会第137回講演会, Mar. 2020
    Poster presentation

  • コールラビ茎肥大性 QTL のファインマッピング
    佐藤 庄人 , モジャミール ホック , シェイ ダニエルジョン, 松村 涼介, 清水 元樹, 藤本 龍, 深井 英吾, 岡崎 桂一
    日本育種学会第137回講演会, Mar. 2020
    Poster presentation

  • Transcriptome analysis before and after salicylic acid treatments in Brassica rapa L.
    Naomi Miyaji, Ryo Fujimoto
    Improvement of important agricultural traits based on genomic information of horticultural crops, Jan. 2020
    Oral presentation

  • Comparison of the Transcriptome profile between F1 hybrid cultivar and it's parental lines at two early developmental stages in Chinese cabbage.
    Mehraj Hasan, Ryo Fujimoto
    Improvement of important agricultural traits based on genomic information of horticultural crops, Jan. 2020
    Oral presentation

  • Characterization of FLOWERLING LOCUS C parlors in a late flowering line of Brassica rapa.
    Ayasha Akter, Ryo Fujimoto
    Improvement of important agricultural traits based on genomic information of horticultural crops, Jan. 2020
    Oral presentation

  • Transcriptome analysis before and after salicylic acid treatments in Brassica rapa L.
    Naomi Miyaji, Takeshi Takasaki-Yasuda, Ryo Fujimoto
    Plant and Animal Genome XXVIII Conference, Jan. 2020, English
    Poster presentation

  • 春化によりキャベツ由来のFLC2がハクサイの遺伝的背景でどのように機能するか
    西田菜美子, シェア ダニエル, 板橋悦子, 安田剛志, 柿崎智博, 岡崎桂一, 藤本龍
    園芸学会令和元年度秋季大会, Sep. 2019
    Oral presentation

  • Comparison of the Transcriptome profile between F1 hybrid cultivar and its parental lines at two early developmental stages in Chinese cabbage
    メエラジ ハサン, 松尾康大, 安田剛志, 藤本龍
    園芸学会令和元年度秋季大会, Sep. 2019
    Oral presentation

  • Characterization of FLOWERING LOCUS C parlors in a late flowering line of Brassica rapa
    アクタ アヤシャ, 西田菜美子, 宮路直実, 安田剛志, 藤本龍
    園芸学会令和元年度秋季大会, Sep. 2019
    Oral presentation

  • ハクサイにおける長鎖非コードRNAとエピジェネティクスとの関係性
    西田菜美子, シェア ダニエル, 高橋聡史, 板橋悦子, アクタ アヤシャ, 宮路直実, メエラジ ハサン, 安田剛志, 柿崎智博, 関原明, 岡崎桂一, 藤本龍
    園芸学会令和元年度秋季大会, Sep. 2019
    Oral presentation

  • ペチュニア花粉におけるニホンナシSFBB融合タンパク質発現系の開発
    上野環太, 久保健一, 藤本龍, 高山誠司, 安田剛志
    園芸学会令和元年度秋季大会, Sep. 2019
    Oral presentation

  • ニホンナシSホモ系統の花粉RNAからのPsSFBB配列の再構築とクローニング
    野木彩未, 高畑宏基, 西村遼太郎, 藤本龍, 安田剛志
    園芸学会令和元年度秋季大会, Sep. 2019
    Oral presentation

  • トランスクリプトーム解析によるシロイヌナズナDDM1変異雑種における雑種強勢低減の改名について
    松尾康大, 宮路直実, 安田剛志, 藤本龍
    日本育種学会第136回講演会, Sep. 2019
    Oral presentation

  • アブラナ科におけるFLCパラログ間の発現量比について
    西田菜美子, アクタ アヤシャ, 板橋悦子, 宮路直実, メエラジ ハサン, 柿崎 智博, 岡崎 桂一, 藤本龍
    日本育種学会第136回講演会, Sep. 2019
    Oral presentation

  • Vernalization alters histone H3 lysine 27 trimethylation at FLC locus in Brassica rapa
    Ayasha Akter, Satoshi Takahashi, Namiko Nishida, Naomi Miyaji, Takeshi Yasuda, Yutaka Suzuki, Motoaki Seki, FUJIMOTO RYO
    2019 KSBB & SABRAO International Conference on Plant Breeding for Sustainable Development, Jul. 2019, English, Kimdaejung Convention Center, Gwangju, International conference
    Poster presentation

  • Identification of non-additively expressed genes at early developmental stages in an F1 hybrid cultivar of Chinese cabbage
    Hasan Mehraj, Kodai Matsuo, Motoki Shimizu, Takeshi Yasuda, FUJIMOTO RYO
    2019 KSBB & SABRAO International Conference on Plant Breeding for Sustainable Development, Jul. 2019, English, Kimdaejung Convention Center, Gwangju, International conference
    Poster presentation

  • Transcriptome analysis indicate mechanisms underlying the DDM1-mediated heterosis in Arabidopsis thaliana
    Kodai Matsuo, Naomi Miyaji, Takeshi Yasuda, FUJIMOTO RYO
    International Symposium; Principles of pluripotent stem cells underlying plant vitality, May 2019, English, Tohoku University, International conference
    Poster presentation

  • Characterization of the transcriptome in heterotic F1 hybrids in Arabidopsis thaliana
    Terumi Murakami, Kodai Matsuo, Naomi Miyaji, Takeshi Yasuda, FUJIMOTO RYO
    International Symposium; Principles of pluripotent stem cells underlying plant vitality 英文, May 2019, English, Tohoku University, International conference
    Poster presentation

  • 萎黄病抵抗性・感受性ハクサイ系統における萎黄病菌感染時の免疫応答
    宮路 直実, 清水 元樹, 加治 誠, 安田 剛志, 岡崎 桂一, FUJIMOTO RYO
    平成31年度日本植物病理学会大会, Mar. 2019, Japanese, つくば国際会議場, Domestic conference
    Oral presentation

  • ハクサイにおける全ゲノムメチル化解析
    FUJIMOTO RYO, 高橋 聡史, 鈴木 穣, 関 原明, 安田 剛志
    園芸学会平成３１年度春季大会, Mar. 2019, Japanese, 明治大学, Domestic conference
    Oral presentation

  • ハクサイにおけるノンコーディングRNAの同定
    西田菜美子, Daniel J Shea, 板橋 悦子, Ayasha Akter, 宮路 直美, Hasan Mehraj, 柿崎 智博, 岡崎 桂一, FUJIMOTO RYO
    日本育種学会第135回講演会, Mar. 2019, Japanese, 千葉大学, Domestic conference
    Oral presentation

  • Distribution of histone H3 lysine 27 trimethylation in different tissues of Brassica rapa
    アクタ アヤシャ, 宮路 直実, 安田 剛志, FUJIMOTO RYO
    園芸学会平成３１年度春季大会, Mar. 2019, English, 明治大学, Domestic conference
    Oral presentation

  • Molecular and cellular characteristics of hybrid vigour in a commercial hybrid of Chinese cabbage.
    FUJIMOTO RYO
    Improvement of important agricultural traits based on genomic information of horticultural crops, Dec. 2018, English, Sunchon National University, International conference
    Public discourse

  • Epigenetics and heterosis
    Kodai Matsuo, FUJIMOTO RYO
    Improvement of important agricultural traits based on genomic information of horticultural crops, Dec. 2018, English, Sunchon National University, International conference
    Public discourse

  • Brassica oleraceaにおける異なる菌株に対する根こぶ病QTL解析
    斎藤 浩之, 小玉 太郎, Mozammel Hoque, Daniel John Shea, 清水 元樹, FUJIMOTO RYO, 深井 英吾, 岡崎 桂一
    第13回東北育種研究集会, Nov. 2018, Japanese, Domestic conference
    Poster presentation

  • アブラナ科野菜における雑種強勢研究の動向
    FUJIMOTO RYO
    平成30年度野菜花き課題別研究会 「アブラナ科野菜における育種と栽培の現状と展望」, Oct. 2018, Japanese, 愛知県産業労働センター, Domestic conference
    Oral presentation

  • ハクサイ初期生育期における子葉および本葉サイズ関連遺伝子の探索
    上園 倖輔, シェア ダニエル, 清水 元樹, 板橋 悦子, ハサン メエラジ, 岡崎 桂一, 安田, 高崎, 剛志, FUJIMOTO RYO
    日本育種学会第134回講演会, Sep. 2018, Japanese, Domestic conference
    Oral presentation

  • ニホンナシSホモ系統における花柱内の和合・不和合花粉管伸長
    高橋侑也, FUJIMOTO RYO, TAKASAKI-YASUDA TAKESHI
    園芸学会平成30年度秋季大会, Sep. 2018, Japanese, 鹿児島大学, Domestic conference
    Oral presentation

  • ニホンナシS3-RNase周辺領域のBACコンティグの拡張によるPpSFBB3の探索
    張競月, 武矢悠太郎, FUJIMOTO RYO, TAKASAKI-YASUDA TAKESHI
    平成30年度園芸学会近畿支部会大阪大会, Sep. 2018, Japanese, 大阪府立大学, Domestic conference
    Poster presentation

  • コールラビ茎肥大性のトランスクリプトーム解析
    ホック モジャミール, シェイ ダニエル, 佐藤 庄人, 松村 涼介, ドウラ モハメドアサドウド, 清水 元樹, FUJIMOTO RYO, 深井 英吾, 岡崎 桂一
    日本育種学会第134回講演会, Sep. 2018, Japanese, Domestic conference
    Poster presentation

  • キャベツにおける葉齢依存的な春化応答性のトランスクリプトーム解析
    板橋 悦子, シェイ ダニエル, 吹野 伸子, FUJIMOTO RYO, 岡崎 桂一, 柿崎 智博, 小原 隆由
    日本育種学会第134回講演会, Sep. 2018, Japanese, Domestic conference
    Poster presentation

  • アブラナ科植物におけるPCR-RFLP法によるSハプロタイプ判定法が直面する問題点
    FUJIMOTO RYO, 川辺 隆大, 岡崎 桂一
    日本育種学会第134回講演会, Sep. 2018, Japanese, Domestic conference
    Oral presentation

  • The molecular basis of vernalization in Brassica rapa
    FUJIMOTO RYO
    Seminar in AgriBio, Sep. 2018, English, La Trobe University, International conference
    Public discourse

  • How Brassica oleracea FLOWERING LOCUS C2 works in a Brassica rapa genetic background
    Namiko Nishida, Daniel J. Shea, Keiichi Okazaki, FUJIMOTO RYO
    AusCanola2018, Sep. 2018, English, Rendezvous Hotel Scarborough, International conference
    Poster presentation

  • Characterization of FLOWERING LOCUS C genes in leafy Brassica rapa vegetables
    FUJIMOTO RYO, Ayasha Akter, Namiko Nishida, Takashi Takasaki-Yasuda, Elizabeth S. Dennis
    AusCanola2018, Sep. 2018, English, Rendezvous Hotel Scarborough, International conference
    Poster presentation

  • Brassica rapaにおけるメチローム解析
    高橋 聡史, 長部 謙二, 宮路 直実, 鈴木 穣, 関 原明, FUJIMOTO RYO
    日本育種学会第134回講演会, Sep. 2018, Japanese, Domestic conference
    Poster presentation

  • Brassica rapa L.における萎黄病・根こぶ病抵抗性DNAマーカー選抜系の応用
    宮路 直実, Hasan Mehraj, 清水 元樹, 加治 誠, 安田, 高崎, 剛志, FUJIMOTO RYO
    平成30年度園芸学会近畿支部大阪大会, Sep. 2018, Japanese, 大阪府立大学 中百舌鳥キャンパス, Domestic conference
    Oral presentation

  • Transcriptome Difference by Fusarium inoculation between Fusarium yellows resistant and susceptible lines in Brassica rapa L.
    Naomi Miyaji, Motoki Shimizu, Takeshi Takasaki-Yasuda, Keiichi Okazaki, FUJIMOTO RYO
    INTERNATIONAL PLANT MOLECULAR BIOLOGY 2018, Aug. 2018, English, International conference
    Poster presentation

  • QTL mapping combined with transcriptome analysis using broccoli and kohlrabi
    Mozammel Hoque, Daniel J. Shea, Masato Sato, Ryosuke Matsumura, Mohamad Asad- ud-doullah, Motoki Shimizu, FUJIMOTO RYO, Eigo Fukai, Keiichi Okazaki
    Brassica 2018, Jul. 2018, English, Saint-Malo, France, International conference
    Poster presentation

  • Characterization of epigenetic states in Brassica rapa L.
    FUJIMOTO RYO, Ayasha Akter, Satoshi Takahashi, Takeshi Takasaki-Yasuda, Yutaka Suzuki, Elizabeth S. Dennis, Motoaki Seki
    Brassica 2018, Jul. 2018, English, Saint-Malo, France, International conference
    Oral presentation

  • Identification of long non-coding RNAs before and after prolonged cold treatment in Brassica rapa L.
    Namiko Nishida, Daniel J. Shea, Satoko Takada, Etsuko Itabashi, Takeshi Takasaki-Yasuda, Keiichi Okazaki, Elizabeth S. Dennis, FUJIMOTO RYO
    The 25th International Congress on Sexual Plant Reproduction, Jun. 2018, English, Nagaragawa Convention Center, International conference
    Poster presentation

  • DDM1 has an important function on heterosis in Arabidopsis thaliana
    Naomi Miyaji, Etsuko Itabashi, Takeshi Takasaki-Yasuda, W. James Peacock, Elizabeth S. Dennis, FUJIMOTO RYO
    The 25th International Congress on Sexual Plant Reproduction, Jun. 2018, English, Nagaragawa Convention Center, International conference
    Poster presentation

  • Characterization of histone H3 lysine 27 tri-methylation in Brassica rapa L.
    FUJIMOTO RYO, Ayasha Akter, Satoshi Takahashi, Namiko Nishida, Takashi Takasaki-Yasuda, Yutaka Suzuki, Motoaki Seki, Elizabeth S. Dennis
    The 25th International Congress on Sexual Plant Reproduction, Jun. 2018, English, Nagaragawa Convention Center, International conference
    Poster presentation

  • 結球野菜の開花調節機構に関する研究の現状と今後の展望について
    FUJIMOTO RYO, 高田 紗都子, 西田 菜美子, 板橋 悦子, 安田 剛志, 柿崎 智博, 宇野 雄一
    園芸学会平成30年度春期大会, Mar. 2018, Japanese, 近畿大学農学部, Domestic conference
    Public symposium

  • 萎黄病菌キャベツ分離菌抵抗性遺伝子DNAマーカーで萎黄病菌コマツナ分離菌抵抗性を予測できるか?
    宮路 直実, 清水 元樹, 加治 誠, 安田 (高, 崎, 剛志, 岡崎 桂一, FUJIMOTO RYO
    園芸学会平成30年度春期大会, Mar. 2018, Japanese, Domestic conference
    Poster presentation

  • ハクサイ市販品種'W77'の両親系統間リシークエンスによる片親特異的遺伝子変異の同定
    上園 倖輔, Shea Daniel, 清水 元樹, 板橋 悦子, 宮路 直美, 岡崎 桂一, 安田, 高崎, 剛志, FUJIMOTO RYO
    園芸学会平成30年度春期大会, Mar. 2018, Japanese, Domestic conference
    Oral presentation

  • キャベツの緑体春化におけるFLOWERING LOCUS Cの役割
    板橋 悦子, 柿崎 智博, 吹野 伸子, 岡崎 桂一, FUJIMOTO RYO, 小原 隆由
    園芸学会平成30年度春期大会, Mar. 2018, Japanese, Domestic conference
    Public symposium

  • LncRNA BrFLC2as in Brassica rapa differs from Arabidopsis thaliana COOLAIR
    Shea Daniel, 高田 紗都子, 西田 菜美子, 板橋 悦子, 清水 元樹, 岡崎 桂一, FUJIMOTO RYO
    日本育種学会第133回講演会, Mar. 2018, Japanese, 九州大学, Domestic conference
    Poster presentation

  • Brassica rapaにおける春化経路関連遺伝子の機能解析
    西田 菜美子, 高田 紗都子, 板橋 悦子, 宮路 直実, 安田 剛志, FUJIMOTO RYO
    園芸学会平成30年度春期大会, Mar. 2018, Japanese, 近畿大学農学部, Domestic conference
    Oral presentation

  • 萎黄病抵抗性タンパク質と相互作用するタンパク質の同定に向けて
    梁瀬 拓雄, 上杉 百花, 神崎 洋之, 清水 元樹, Daniel J Shea, FUJIMOTO RYO, 深井 英吾, 岡崎 桂一
    第１２回東北育種研究集会, Nov. 2017, Japanese, 秋田県立大学, Domestic conference
    Poster presentation

  • Brassica rapaにおける萎黄病抵抗性遺伝子FocBr1の多型解析と選抜マーカーの開発
    佐藤 真帆, Daniel J. Shea, 清水 元樹, FUJIMOTO RYO, 深井 英吾, 岡崎 桂一
    第１２回東北育種研究集会, Nov. 2017, Japanese, 秋田県立大学, Domestic conference
    Poster presentation

  • Re-sequencing and SNP analysis of the parental lines of a commercial F1 hybrid cultivar of Chinese cabbage
    Daniel Shea, Motoki Shimizu, Etsuko Itabashi, Naomi Miyaji, Makoto Kaji, Keiichi Okazaki, FUJIMOTO RYO
    日本育種学会第132回講演会, Oct. 2017, English, Domestic conference
    Poster presentation

  • Brassica oleracea FLC2領域を移入交雑で導入したBrassica rapaの特性解析
    シェイ ダニエル, 戸丸 祐貴, 板橋 悦子, 宮崎 俊夫, 柿崎 智博, 清水 元樹, FUJIMOTO RYO, 深井 英吾, 岡崎 桂一
    日本育種学会第132回講演会, Oct. 2017, English, Domestic conference
    Oral presentation

  • 萎黄病抵抗性•感受性ハクサイ系統における萎黄病菌感染時転写応答の解明
    宮路 直実, 清水 元樹, 高田 紗都子, 加治 誠, 安田, 高崎, 剛志, 岡崎 桂一, FUJIMOTO RYO
    園芸学会平成29年度秋期大会, Sep. 2017, Japanese, Domestic conference
    Oral presentation

  • ハクサイ市販品種'W77'の両親系統のリシークエンスによるゲノム構造の系統間比較
    上園 倖輔, Shea Daniel, 清水 元樹, 板橋 悦子, 譚 永, 宮路 直美, 岡崎 桂一, 安田, 高崎, 剛志, FUJIMOTO RYO
    平成29年度園芸学会近畿支部滋賀大会, Sep. 2017, Japanese, Domestic conference
    Poster presentation

  • ハクサイ市販品種'W77'における初期生育の雑種強勢関連領域の同定にむけて
    譚 永, 上園 倖輔, 板橋 悦子, 安田, 高崎, 剛志, FUJIMOTO RYO
    平成29年度園芸学会近畿支部滋賀大会, Sep. 2017, Japanese, ピアザ淡海, Domestic conference
    Oral presentation

  • ニホンナシS2およびS4ハプロタイプ間のゲノム構造比較
    西村 遼太郎, 安藤 肇, FUJIMOTO RYO, 安田, 剛志
    園芸学会平成29年度秋期大会, Sep. 2017, Japanese, Domestic conference
    Oral presentation

  • MeDIP-seqによるハクサイ系統間のDNAメチル化比較解析
    FUJIMOTO RYO, 高橋 聡史, 鈴木 穣, 関 原明, 安田 剛志
    園芸学会平成29年度秋期大会, Sep. 2017, Japanese, Domestic conference
    Oral presentation

  • Brassica rapa L.における春化によるFLCのエピジェネティックな修飾変化の系統間比較
    高田 紗都子, 板橋 悦子, 宮路 直実, 安田 剛志, FUJIMOTO RYO
    園芸学会平成29年度秋期大会, Sep. 2017, Japanese, Domestic conference
    Oral presentation

  • Similar but different: the molecular basis of vernalization in Brassica rapa
    FUJIMOTO RYO
    THE PLANT EPIGENETICS CONSORTIUM IN JAPAN- SECOND MEETING, Aug. 2017, English, Domestic conference
    Oral presentation

  • DDM1 is required for a full level of heterosis in Arabidopsis thaliana
    Naomi Miyaji, Sonoko Ishikura, Satoko Takada, Takeshi Takasaki-Yasuda, W. James Peacock, Elizabeth S. Dennis, FUJIMOTO RYO
    Plant Biology 2017, Jun. 2017, English, International conference
    Poster presentation

  • ニホンナシ柱頭の表面および微細構造の変化と柱 頭浸出液の出現について
    高橋 侑也, 李 麗怡, 岡村 彩葉, FUJIMOTO RYO, 安田 (高, 崎, 剛志
    医学生物学電子顕微鏡技術学会第33回学術講演会, May 2017, Japanese, Domestic conference
    Oral presentation

  • Brassica rapa L. におけるアブラナ科 植物白さび病抵抗性遺伝子の探索と検 出
    西本 良太, 宮路 直実, FUJIMOTO RYO, 土佐 幸雄, 中馬 いづみ
    平成29年度日本植物病理学会大会, Apr. 2017, Japanese, Domestic conference
    Oral presentation

  • トランスクリプトーム解析を用いたハクサイの萎黄病菌感染時における免疫応答調査
    宮路 直実, 清水 元樹, 高田 紗都子, 加治 誠, 安田 剛志, 岡崎 桂一, FUJIMOTO RYO
    日本育種学会第131回講演会, Mar. 2017, Japanese, 名古屋大学, Domestic conference
    Oral presentation

  • シロイヌナズナの早期開花エピ変異体の形質評価
    高田 紗都子, 石倉 園子, 板橋 悦子, 宮路 直実, 清水 元樹, 安田 剛志, FUJIMOTO RYO
    日本育種学会第131回講演会, Mar. 2017, Japanese, 名古屋, Domestic conference
    Oral presentation

  • アブラナ科植物におけるゲノム多様性および雑種強勢に関する研究
    FUJIMOTO RYO
    日本育種学会第131回講演会, Mar. 2017, Japanese, 名古屋大学, Domestic conference
    [Invited]
    Invited oral presentation

  • Brassica oleraceaにおける萎黄病抵抗性遺伝子FocBo1の多型解析と選抜マーカー開発
    佐藤 真帆, 清水 元樹, FUJIMOTO RYO, 深井 英吾, 岡崎 桂一
    日本育種学会第131回講演会, Mar. 2017, Japanese, 名古屋大学, Domestic conference
    Poster presentation

  • The epigenetic regulation of FLC expression by vernalization in Brassica rapa L
    Satoko Takada, Etsuko Itabashi, Naomi Miyaji, Takeshi Takasaki-Yasuda, Kenji Osabe, Elizabeth S. Dennis, FUJIMOTO RYO
    Brassica 2016, Oct. 2016, English, International conference
    Poster presentation

  • QTL mapping for stem swelling trait of Kohlrabi (Brassica oleracea var. gongylodes)
    Mozammel Hoque, Mitsuru Asada, Daniel Shea, Motoki Shimizu, FUJIMOTO RYO, Eigo Fukai, Keiichi Okazaki
    Brassica 2016, Oct. 2016, English, International conference
    Oral presentation

  • Identification of the Fusarium yellows resistance genes; its application for marker-assisted selection in Brassica rapa
    Naomi Miyaji, Motoki Shimizu, Satoko Takada, Takeshi Takasaki-Yasuda, Makoto Kaji, Kenji Osabe, Elizabeth S. Dennis, Keiichi Okazaki, FUJIMOTO RYO
    Brassica 2016, Oct. 2016, English, International conference
    Oral presentation

  • Genome-wide analysis of DNA methylation in Chinese cabbage
    Naoki Fukushima, Satoshi Takahashi, Etsuko Itabashi, Motoki Shimizu, Kenji Osabe, Takeshi Yasuda, Yutaka Suzuki, Motoaki Seki, Elizabeth S. Dennis, FUJIMOTO RYO
    Brassica 2016, Oct. 2016, English, International conference
    Poster presentation

  • Characterization of early developmental and yield heterosis in Chinese cabbage
    FUJIMOTO RYO, Motoki Shimizu, Naomi Miyaji, Satoko Takada, Naoki Fukushima, Makoto Kaji, W. James Peacock, Elizabeth S. Dennis
    Brassica 2016, Oct. 2016, English, International conference
    Oral presentation

  • 萎黄病菌を接種したときの抵抗性と感受性ハクサイ系統のトランスクリプトームプロファイリング
    宮路 直実, 清水 元樹, 高田 紗都子, 加治 誠, 安田 剛志, 岡崎 桂一, FUJIMOTO RYO
    日本育種学会第130回講演会, Sep. 2016, Japanese, 鳥取大学, Domestic conference
    Poster presentation

  • ハクサイにおける萎黄病菌感染時のトランスクリプトーム解析による病害反応遺伝子の調査
    宮路 直実, 清水 元樹, 高田 紗都子, 加治 誠, 安田 剛志, 岡崎 桂一, FUJIMOTO RYO
    園芸学会平成28年度秋季大会, Sep. 2016, Japanese, 名城大学, Domestic conference
    Oral presentation

  • ハクサイにおけるメチローム解析
    福島 尚樹, 高橋 聡史, 板橋 悦子, 清水 元樹, 安田 剛志, 鈴木 穣, 関 原明, FUJIMOTO RYO
    日本育種学会第130回講演会, Sep. 2016, Japanese, Domestic conference
    Oral presentation

  • ハクサイにおけるエピゲノム解析
    FUJIMOTO RYO, 高橋 聡史, 板橋 悦子, 鈴木 穣, 関 原明
    日本育種学会第130回講演会, Sep. 2016, Japanese, 鳥取大学, Domestic conference
    Public symposium

  • ニホンナシS3-RNase 周辺BACコンティグの塩基配列解析
    武矢 悠太郎, 野村 直希, FUJIMOTO RYO, 安田, 剛志
    園芸学会平成28年度秋季大会, Sep. 2016, Japanese, 名城大学, Domestic conference
    Oral presentation

  • ニホンナシS2遺伝子座のゲノム構造および発現遺伝子解析
    西村 遼太郎, FUJIMOTO RYO, 安田, 剛志
    園芸学会平成28年度秋季大会, Sep. 2016, Japanese, 名城大学, Domestic conference
    Oral presentation

  • シロイヌナズナの雑種強勢に関与する遺伝子領域の探索
    宮路 直実, 石倉 園子, 高田 紗都子, 板橋 悦子, 安田 剛志, FUJIMOTO RYO
    日本育種学会第130回講演会, Sep. 2016, Japanese, Domestic conference
    Oral presentation

  • クロマチンリモデリング因子DDM1によるエピジェネティックな制御がシロイヌナズナのヘテローシスに及ぼす影響
    石倉 園子, 宮路 直実, 板橋 悦子, 高田 紗都子, 高橋 聡史, 清水 元樹, 安田 剛志, 関 原明, FUJIMOTO RYO
    日本育種学会第130回講演会, Sep. 2016, Japanese, 鳥取大学, Domestic conference
    Oral presentation

  • アブラナ科植物白さび病菌Albugo candidaに対する抵抗性遺伝子同定に向けたBrassica rapa抵抗性品種の選抜
    西本 良太, 宮路 直実, FUJIMOTO RYO, 土佐 幸雄, 中馬 いづみ
    日本育種学会第130回講演会, Sep. 2016, Japanese, 鳥取大学, Domestic conference
    Oral presentation

  • Whole genome bisulfite sequencingによるハクサイのDNAメチル化領域の探索
    福島 尚樹, 高橋 聡史, 板橋 悦子, 清水 元樹, 安田 剛志, 鈴木 穣, 関 原明, FUJIMOTO RYO
    園芸学会平成28年度秋季大会, Sep. 2016, Japanese, 名城大学, Domestic conference
    Oral presentation

  • Brassica rapa L.の開花期の決定におけるFRIGIDAとFLOWERING LOCUS Cの役割
    高田 紗都子, 板橋 悦子, 宮路 直実, 安田 剛志, FUJIMOTO RYO
    日本育種学会第130回講演会, Sep. 2016, Japanese, Domestic conference
    Oral presentation

  • Brassica rapa L.における春化によるFLOWERING LOCUS C のエピジェネティックな転写制御について
    高田 紗都子, 板橋 悦子, 宮路 直実, 清水 元樹, 安田 剛志, FUJIMOTO RYO
    園芸学会平成28年度秋季大会, Sep. 2016, Japanese, 名城大学, Domestic conference
    Poster presentation

  • Brassica rapa L.における開花関連遺伝子FRIGIDA の発現解析および全長配列の決定
    高田 紗都子, 板橋 悦子, 宮路 直実, 安田 剛志, FUJIMOTO RYO
    園芸学会平成28年度秋季大会, Sep. 2016, Japanese, 名城大学, Domestic conference
    Oral presentation

  • ニホンナシS3-RNase 周辺領域451kb のゲノム構造解析
    武矢 悠太郎, 野村 直希, FUJIMOTO RYO, 安田 剛志
    平成28 年度園芸学会近畿支部兵庫大会, Aug. 2016, Japanese, Domestic conference
    Oral presentation

  • ニホンナシS2-RNase 周辺領域663kb のゲノム構造解析
    西村 遼太郎, FUJIMOTO RYO, 安田 剛志
    平成28 年度園芸学会近畿支部兵庫大会, Aug. 2016, Japanese, 神戸大学 瀧川記念学術交流会館, Domestic conference
    Oral presentation

  • Brassica rapa における白さび病抵抗性遺伝子同定に向けた病害反応調査
    宮路 直美, 西本 良太, 清水 元樹, 高田 紗都子, 安田 剛志, 土佐 幸雄, 中馬 いづみ, FUJIMOTO RYO
    平成28 年度園芸学会近畿支部兵庫大会, Aug. 2016, Japanese, 神戸大学 瀧川記念学術交流会館, Domestic conference
    Oral presentation

  • Brassica rapa L.における春化経路関連遺伝子FLOWERING LOCUS C の発現解析および エピジェネティックな転写制御について
    高田 紗都子, 板橋 悦子, 宮路 直美, 安田 剛志, FUJIMOTO RYO
    平成28 年度園芸学会近畿支部兵庫大会, Aug. 2016, Japanese, Domestic conference
    Oral presentation

  • シロイヌナズナの雑種強勢の分子機構の解明に向けたRILの整備
    川辺 隆大, 宮路 直美, 高田 紗都子, 板橋 悦子, 安田 剛志, FUJIMOTO RYO
    日本育種学会第129回講演会, Mar. 2016, Japanese, 横浜市立大学, Domestic conference
    Oral presentation

  • ハクサイにおけるヒストンメチル化抗体を用いたクロマチン免疫沈降法のポジティブ ネガティブコントロールプライマーの作製
    FUJIMOTO RYO, 川辺 隆大, 板橋 悦子, 安田 剛志
    平成27年度園芸学会秋季大会, Sep. 2015, Japanese, 園芸学会, 徳島大学, 概要, Domestic conference
    Oral presentation

  • ハクサイにおけるヒストンメチル化抗体を用いたクロマチン免疫沈降法のポジティブ/ネガティブコントロールプライマーの作製
    FUJIMOTO RYO, 川辺 隆大, 板橋 悦子, 安田 剛志, 岡崎 桂一
    園芸学会平成27年度秋季大会, Sep. 2015, Japanese, Domestic conference
    Oral presentation

  • ハクサイにおいて、両親系統間の遺伝距離から雑種強勢を予測できるか?
    川村 和隆, 佐伯 なつみ, 今野 周平, 永野 敦, 本庄 三恵, 八杉 公基, 工藤 洋, 安田 剛志, 加治 誠, 岡崎 桂一, FUJIMOTO RYO
    日本育種学会第128回講演会, Sep. 2015, Japanese, Domestic conference
    Oral presentation

  • ハクサイにおいて、両親系統間の遺伝距離から雑種強勢を予測できるか?
    川村 和隆, 佐伯 なつみ, 今野 周平, 永野 敦, 本庄 三恵, 八杉 公基, 工藤 洋, 安田 剛志, 加治 誠, 岡崎 桂一, FUJIMOTO RYO
    育種学会第128回講演会, Sep. 2015, Japanese, 日本育種学会, 新潟大学, 概要, Domestic conference
    Oral presentation

  • ニホンナシSホモ系統の花粉cDNAからのPpSFBB2ホモログのクローニングII
    西村 遼太郎, FUJIMOTO RYO, 安田 剛志
    平成27年度秋季大会, Sep. 2015, Japanese, 園芸学会, 徳島大学, 概要, Domestic conference
    Oral presentation

  • アブラナ科の雑種強勢の分子機構の解明を目指した後成遺伝学的なアプローチ
    FUJIMOTO RYO, 川辺 隆大
    日本遺伝学会第87大会, Sep. 2015, Japanese, 東北大学, Domestic conference
    [Invited]
    Invited oral presentation

  • Brassica oleraceaの倍加半数体系統群を用いた連鎖地図の作成と根こぶ病レース特異的CR-QTLの同定
    小玉 太郎, 川村 和隆, モハマドアサドウドドウラ, 清水 元樹, FUJIMOTO RYO, 深井 英吾, 岡崎 桂一
    日本育種学会第128回講演会, Sep. 2015, Japanese, Domestic conference
    Poster presentation

  • Brassica oleracea における根こぶ病抵抗性遺伝子PbBo(Anju)1 のファインマッピング
    川村 和隆, 小玉 太郎, 清水 元樹, モハマドアサドウドドウラ, 富田 寛也, 佐々木 一嘉, FUJIMOTO RYO, 深井 英吾, 岡崎 桂一
    日本育種学会第128回講演会, Sep. 2015, Japanese, Domestic conference
    Oral presentation

  • Genetic and epigenetic approaches for identification of molecular mechanism of heterosis in Brassica
    FUJIMOTO RYO
    A consortium of plant epigenetisists in Japan – First Meeting, Jul. 2015, English, National Institute of Genetics, Domestic conference
    Public discourse

  • DNA marker-assisted breeding in F1 hybrid cultivar of Chinese cabbage
    FUJIMOTO RYO
    Symposium; Epigenetics and Gene expression for Crop improvement, Apr. 2015, English, ANU university House, International conference
    Oral presentation

  • Genetic and epigenetic approaches for identification of molecular mechanism of heterosis in Brassica
    FUJIMOTO RYO
    Institute of Plant Science and Recourses Workshop; Plant Epigenetics: From Emerging Phenomena to Novel Molecular Events, Jan. 2015, English, Kurashiki Geibun kan, Domestic conference
    Oral presentation

  • ハクサイ市販一代雑種品種‘W77’における雑種強勢に関わるQTLの探索
    阿部 寛史, 川辺 隆大, 佐伯 なつみ, 清水 元樹, 今野 周平, 永野 惇, 本庄 三恵, 八杉 公基, 工藤 洋, 畠山 勝徳, 松元 哲, 岡崎 桂一, 加治 誠, FUJIMOTO RYO
    日本育種学会第126回講演会, Sep. 2014, Japanese, 南九州大学, Domestic conference
    Oral presentation

  • ニホンナシ和合・不和合花粉管へのS-RNaseの取り込み
    李 麗怡, 時安 美奈, FUJIMOTO RYO, 安田 剛志
    平成26年度秋季大会, Sep. 2014, Japanese, 園芸学会, 佐賀大学, Domestic conference
    Oral presentation

  • ニホンナシSホモ系統の花粉cDNAからのPpSFBB2ホモログのクローニング
    西村 遼太郎, 今村 剛士, 加藤 大貴, FUJIMOTO RYO, 安田 剛志
    平成26年度秋季大会, Sep. 2014, Japanese, 園芸学会, 佐賀大学, Domestic conference
    Oral presentation

  • ニホンナシS4-RNase周辺BACコンティグの拡張によるPpSFBB遺伝子群の探索
    安藤 肇, 山下 翔人, FUJIMOTO RYO, 安田 剛志
    平成26年度秋季大会, Sep. 2014, Japanese, 園芸学会, 佐賀大学, Domestic conference
    Oral presentation

  • ニホンナシnon-S-RNaseの花柱組織における蓄積と花粉管への取り込み
    時安 美奈, 李 麗怡, 岡村 彩葉, FUJIMOTO RYO, 安田 剛志
    平成26年度秋季大会, Sep. 2014, Japanese, 園芸学会, 佐賀大学, Domestic conference
    Oral presentation

  • シロイヌナズナの雑種強勢に概日リズムは関わるか?
    川辺 隆大, 佐伯 なつみ, 阿部 寛史, FUJIMOTO RYO
    日本育種学会第126回講演会, Sep. 2014, Japanese, 南九州大学, Domestic conference
    Poster presentation

  • アブラナ科萎黄病菌(Fusarium oxysporum)のde novo全ゲノムシークエンス情報を用いた分化型特異的遺伝子領域の探索
    船木 武人, 田子 明日美, Pu ZI-JING, 深井 英吾, 佐野 義孝, 阿部 貴志, 藤 晋一, FUJIMOTO RYO, 清水 元樹, 夏目 俊, 寺内 良平, 岡崎 桂一
    日本育種学会第126回講演会, Sep. 2014, Japanese, 南九州大学, Domestic conference
    Poster presentation

  • DNAマーカーを用いたBrassica rapa近交系の基盤整備
    川村 和隆, 吉田 実央, 清水 元樹, 佐伯 なつみ, 阿部 寛史, 江部 裕介, 岡崎 桂一, FUJIMOTO RYO, 川辺 隆大
    日本育種学会第126回講演会, Sep. 2014, Japanese, 南九州大学, Domestic conference
    Poster presentation

  • Brassica oleracea近交系の遺伝的均一性評価およびF1種子純度検定に適したDNAマーカーの探索
    川村 和隆, 清水 元樹, 佐伯 なつみ, 阿部 寛史, 江部 裕介, 岡崎 桂一, 川辺 隆大, FUJIMOTO RYO
    園芸学会平成26年度秋期大会, Sep. 2014, Japanese, Domestic conference
    Poster presentation

  • ハクサイ市販一代雑種品種‘W39’における雑種強勢関連遺伝子の発現解析
    佐伯 なつみ, 川辺 隆大, 野瀬 笙子, 阿部 寛史, 加治 誠, 岡崎 桂一, FUJIMOTO RYO
    園芸学会平成26年度春季大会, Mar. 2014, Japanese, 筑波大学, Domestic conference
    Oral presentation

  • ハクサイ市販一代雑種品種W77及びそのF2後代の形質調査
    阿部 寛史, 川辺 隆大, 野瀬 笙子, 佐伯 なつみ, 清水 元樹, 今野 周平, 加治 誠, 岡崎 桂一, FUJIMOTO RYO
    日本育種学会第125回講演会, Mar. 2014, Japanese, 東北大学, Domestic conference
    Oral presentation

  • シロイヌナズナの初期生育時に見られる雑種強勢はPol4の機能とは独立である
    川辺 隆大, 佐伯 なつみ, 佐々木 卓, 阿部 寛史, FUJIMOTO RYO
    日本育種学会第125回講演会, Mar. 2014, Japanese, 日本育種学会, Domestic conference
    Poster presentation

  • RNA-sequencing法によるハクサイ市販F1品種の両親系統間のSNP同定
    佐伯なつみ, 野瀬 笙子, 川辺 隆大, 阿部 寛史, 岡崎 桂一, 加治 誠, FUJIMOTO RYO
    日本育種学会第125回講演会, Mar. 2014, Japanese, 東北大学, Domestic conference
    Oral presentation

  • Differential expression解析を用いたハクサイ萎黄病抵抗性候補遺伝子の同定
    清水 元樹, 江部 裕介, 川辺 隆大, 佐伯 なつみ, 加治 誠, 岡崎 桂一, FUJIMOTO RYO
    園芸学会平成26年度春季大会, Mar. 2014, Japanese, 筑波大学, Domestic conference
    Poster presentation

  • Brassica oleraceaにおける近交系の純度検定に最適なDNAマーカーのスクリーニングと汎用性
    川村 和隆, 玄田 真優, 佐々木 一嘉, 清水 元樹, 佐伯 なつみ, 岡崎 桂一, FUJIMOTO RYO, 川辺 隆大
    日本育種学会第125回講演会, Mar. 2014, Japanese, 東北大学, Domestic conference
    Poster presentation

  • ハクサイ市販F1品種‘W39’の生育初期に見られる雑種強勢について
    佐伯 なつみ, 川辺 隆大, 阿部 寛史, 加治 誠, 岡崎 桂一, FUJIMOTO RYO
    日本育種学会第124回講演会, Oct. 2013, Japanese, 鹿児島大学, Domestic conference
    Oral presentation

  • シロイヌナズナの子葉における雑種強勢を示すF1とその両親系統間での転写比較解析
    FUJIMOTO RYO, TAYLOR M. JENNIFER, 川辺 隆大, 佐伯 なつみ, 阿部 寛史, PEACOCK J. WILLIAM, DENNIS S. ELIZABETH
    日本育種学会第124回講演会, Oct. 2013, Japanese, 鹿児島大学, Domestic conference
    Oral presentation

  • シロイヌナズナのF1雑種にみられる雑種強勢の程度について
    川辺 隆大, 井出 千賀, 佐伯 なつみ, FUJIMOTO RYO
    日本育種学会第124回講演会, Oct. 2013, Japanese, 鹿児島大学, Domestic conference
    Oral presentation

  • アブラナ科植物一代雑種品種における両親系統の対立遺伝子間相互作用
    川辺 隆大, 佐伯 なつみ, 阿部 寛史, 今野 周平, 加治 誠, 岡崎 桂一, FUJIMOTO RYO
    日本育種学会第124回講演会, Oct. 2013, Japanese, 鹿児島大学, Domestic conference
    Public symposium

  • Brassica oleracea品種における萎黄病抵抗性遺伝子FocBo1連鎖DNA マーカーと抵抗性との関連性
    清水 元樹, 川辺 隆大, PU ZI-JING, FUJIMOTO RYO, 岡崎 桂一
    日本育種学会第124回講演会, Oct. 2013, Japanese, 鹿児島大学, Domestic conference
    Poster presentation

  • Brassica oleracea染色体添加型 B. rapa を用いた緑植物体春化の遺伝解析
    戸丸 祐貴, 中村 友理, 清水 元樹, FUJIMOTO RYO, 岡崎 桂一
    日本育種学会第124回講演会, Oct. 2013, Japanese, 鹿児島大学, Domestic conference
    Oral presentation

  • 初期生育時に見られるハクサイとシロイヌナズナの雑種強勢の相違点
    FUJIMOTO RYO, 佐伯 なつみ, 阿部 寛史, 加治 誠, 川辺 隆大
    園芸学会平成25年度秋期大会, Sep. 2013, Japanese, 岩手大学, Domestic conference
    Poster presentation

  • ハクサイF1品種W77における初期生育と収穫期の雑種強勢の評価
    川辺 隆大, 阿部 寛史, 佐伯 なつみ, 清水 元樹, 今野 周平, 加治 誠, FUJIMOTO RYO
    園芸学会平成25年度秋期大会, Sep. 2013, Japanese, 岩手大学, Domestic conference
    Oral presentation

  • ハクサイF1品種W39における生育初期及び収穫期の雑種強勢の評価
    佐伯 なつみ, 川辺 隆大, 阿部 寛史, 今野 周平, 清水 元樹, 加治 誠, FUJIMOTO RYO
    園芸学会平成25年度秋期大会, Sep. 2013, Japanese, 岩手大学, Domestic conference
    Oral presentation

  • Variation of the level of the heterosis among F1 hybrid individuals in Arabidopsis thaliana
    KAWANABE TAKAHIRO, SAEKI NATSUMI, FUJIMOTO RYO
    24th International Conference on Arabidopsis Research, Jun. 2013, English, Sydney, Australia, International conference
    Poster presentation

  • A large cotyledon area after germination is a common phenomenon in heterotic F1 hybrids of Arabidopsis thaliana and Chinese cabbage
    SAEKI NATSUMI, KAWANABE TAKAHIRO, ABE HIROFUMI, SHIMIZU MOTOKI, KONNO SHUHEI, KAJI MAKOTO, FUJIMOTO RYO
    24th International Conference on Arabidopsis Research, Jun. 2013, English, Sydney, Australia, International conference
    Poster presentation

  • アブラナ科植物の種内、種間雑種に見られる非相加的遺伝子発現
    FUJIMOTO RYO
    日本育種学会第123回講演会, Mar. 2013, Japanese, 東京農業大学, Domestic conference
    [Invited]
    Invited oral presentation

  • Establishment of heterosis at early developmental stage in Arabidopsis thaliana is independent from Pol4 activity
    FUJIMOTO RYO, SASAKI TAKU, KAWANABE TAKAHIRO, SAEKI NATSUMI, ABE HIROFUMI, PEACOCK J. WILLIAM, DENNIS S. ELIZABETH
    第54回日本植物生理学会年会, Mar. 2013, English, 岡山大学, Domestic conference
    Oral presentation

  • Recent progress of clubroot resistance breeding through marker-assisted selection in Brassica oleracea and B. rapa
    Doullah MAU, Tomita H, Shimizu M, Matsumoto S, Fujimoto R, Okazaki K
    5th International symposium for the development of integrated pest management for sustainable agriculture in Asia and Africa, English
    Oral presentation

  • Analysis of QTLs controlling black rot (Xanthomonas campestris pv. campestris) resistance in Brassica oleracea
    Tonu NN, Doullah MAU, Shimizu M, Karim MM, Fujimoto R, Okazaki K
    5th International symposium for the development of integrated pest management for sustainable agriculture in Asia and Africa, Dec. 2012, English
    Oral presentation

  • Identification of Brassica rapa fusarium wilt resistance gene, FocBr1, by differential expression analysis of NBS-LRR motif genes
    Shimizu M, Ebe Y, Kawanabe T, Pu Z, Fujimoto R, Kaji M, Zhang Y, Sano Y, Okazaki K
    5th International symposium for the development of integrated pest management for sustainable agriculture in Asia and Africa, Dec. 2012, English
    Poster presentation

  • Strong Heterosis in Yield is Seen in Chinese Cabbage F1 Hybrid Cultivars
    Tomaru, Y, Kawanabe, T, Abe, H, Saeki, N, Konno, S, Shimizu, M, Tomita, H, Okazaki, K, Kaji, M, Dennis, E,S, Fujimoto, R
    6th International symposium on Brassica and 18th Crucifer Genetics Workshop, Nov. 2012, English
    Poster presentation

  • Identification of Brassica rapa homologue of fusarium wilt resistance gene, Foc-Bo1 by genome synteny analysis between B. oleracea and B. rapa
    Shimizu M, Ebe Y, Kawanabe T, Pu Z, Fujimoto R, Kaji M, Zhang Y, Okazaki K
    6th International symposium on Brassica and 18th Crucifer Genetics Workshop, Nov. 2012, English
    Poster presentation

  • Towards cloning a clubroot resistance minor gene PbBo(Anju)4 in Brassica oleracea
    Tomita, Motoki Shimizu, Md. Asad ud-doullah, Ryo Fujimoto, Keiichi Okazaki
    International symposium on Comparative Genomics and Breeding of Brassicaceae crops, Oct. 2012, English
    Poster presentation

  • Collinearity among a short segment having the major recognition gene complex of Arabidopsis thaliana chromosome 4 and triplicated regions within the Brassica oleracea and B. rapa
    Motoki Shimizu, Hiroya Tomita, Pu Zijing, Ryo Fujimoto, Keiichi Okazaki
    International symposium on Comparative Genomics and Breeding of Brassicaceae crops, Oct. 2012, English
    Poster presentation

  • Non-additive gene regulation in heterotic F1 hybrids of A. thaliana
    Satoko Matsuoka, Takahiro Kawanabe, Jennifer M. Taylor, W. James Peacock, Elizabeth S. Dennis, Ryo Fujimoto
    International symposium on Comparative Genomics and Breeding of Brassicaceae crops, Oct. 2012, English
    Poster presentation

  • Characterization of the early developmental heterosis phenotype of the commercial F1 hybrid cultivars in Chinese cabbage
    Yuki Ishizaki, Hirofumi Abe, Natsumi Saeki, Takahiro Kawanabe, Shuhei Konno, Makoto Kaji, Hua Ying, Elizabeth S. Dennis, Ryo Fujimoto
    International symposium on Comparative Genomics and Breeding of Brassicaceae crops, Oct. 2012, English
    Poster presentation

  • Characterization of NBS-LRR motif genes between S11 and R09 by RNA sequencing in Brassica rapa
    Motoki Shimizu, Hua Ying, Ryo Fujimoto, Yusuke Ebe, Hiroya Tomita, Takahiro Kawanabe, Makoto Kaji, Elizabeth S. Dennis, Keiichi Okazaki
    10th International Congress on Plant Molecular Biology, Oct. 2012, English
    Poster presentation

  • Towards identifying a fusarium wilt resistance gene in Chinese cabbage (Brassica rapa ssp. pekinensis)
    Tomita, H, Shimizu, M, Ebe, Y, Ying, H, Kawanabe, T, Kaji, M, Fujimoto,R, Dennis, E.S, Okazaki, K
    10th International Congress on Plant Molecular Biology, Oct. 2012, English
    Poster presentation

  • on-additive gene regulation in heterotic F1 hybrids of Arabidopsis thaliana and Chinese cabbage
    川辺隆大, Ying H, Taylor JM, 佐伯なつみ, 阿部寛史, Dennis ES, 藤本龍
    日本育種学会, Sep. 2012
    Poster presentation

  • RNA-sequence法を用いたハクサイ品種間（S11×R09）特異的なNBS-LRRモチーフ遺伝子の探索
    清水 元樹, 江部 裕介, 藤本 龍, 川邊 隆大, Hua Ying, 加治 誠, Elizabeth Dennis, 岡崎 桂一
    日本育種学会, Sep. 2012
    Oral presentation

  • The genetic association of clubroot and fusarium wild disease resistance gene between Brassica oleracea and Brassica rapa based on expression sequence tagged comparative genomics.
    Shimizu M, Pu Z, Tomita H, Fujimoto R, Okazaki K
    The 4th International Symposium on ‘‘Modern Agriculture and Rural Economic Development in Asia’’, Jul. 2012, English
    Poster presentation

  • Development of recombinant inbred line to clone three minor clubroot resistance genes in Brassica oleracea
    Tomita H, Ud-doullah MA, Shimizu M, Fujimoto R, Okazaki K
    The 4th International Symposium on ‘‘Modern Agriculture and Rural Economic Development in Asia’’, Jul. 2012, English
    Poster presentation

  • Early developmental heterosis are observed in F1 hybrid cultivar ‘W39’ of Chinese cabbage
    Saeki N, Abe H, Kawanabe T, Konno S, Shimizu M, Tomita H, Kaji M, Dennis ES, Fujimoto R
    The 4th International Symposium on ‘‘Modern Agriculture and Rural Economic Development in Asia’’, Jul. 2012, English
    Poster presentation

  • Characterization of the heterosis phenotype of the commercial F1 hybrid cultivar ‘W77’ in Chinese cabbage
    Abe H, Saeki N, Kawanabe T, Konno S, Shimizu M, Tomita H, Kaji M, Dennis ES, Fujimoto R
    The 4th International Symposium on ‘‘Modern Agriculture and Rural Economic Development in Asia’’, Jul. 2012, English
    Poster presentation

  • Stress response genes are differentially expressed both between related Arabidopsis species and in their amphidiploid
    Kawanabe Takahiro, Sasaki Taku, Taylor Jenifer, Dennis Elizabeth, Fujimoto Ryo
    植物生理学会, Mar. 2012
    Poster presentation

  • 強いヘテローシスは、ハクサイの一代雑種品種の収量に見られる
    川辺隆大, 今野周平, 阿部寛史, 清水元樹, 富田寛也, 加治誠, Elizabeth S. Dennis, 藤本龍
    日本育種学会, Mar. 2012
    Oral presentation

  • Brassica oleracea根こぶ病抵抗性遺伝子の集積による抵抗性育種
    富田寛也, 清水元樹, 藤本龍, U. Doullah MA, 松本哲, 岡崎桂一
    日本育種学会, Mar. 2012
    Oral presentation

  • キャベツ萎黄病抵抗性遺伝子Foc-Bo1の同定とファインマッピング
    清水元樹, Pu Zi-Jing, 富田寛也, 藤本龍, 松本哲, 林武司, Zhang Yan-ju, 岡崎桂一
    日本育種学会, Mar. 2012
    Oral presentation

  • Epigenetics and heterosis
    Greaves IK, Groszmann M, Fujimoto R, Dennis ES, Peacock WJ
    XXII International Congress on Sexual Plant Reproduction. Melbourne, Feb. 2012, English
    Oral presentation

  • Early developmental heterosis in both Arabidopsis thaliana and Chinese cabbage
    Fujimoto R, Taylor JM, Kawanabe T, Konno S, Shirasawa S, Peacock WJ, Dennis ES
    XXII International Congress on Sexual Plant Reproduction, Feb. 2012, English
    Oral presentation

  • The cumulative effect of five Clubroot resistant genes in Brassica oleracea
    Tomita, H, Shimizu, M, Fujimoto, R, Ud-doullah, M.A, Matsumoto, S, Okazaki, K
    PLANT & ANIMAL GENOME XX, Jan. 2012, English
    Poster presentation

  • シロイヌナズナの初期生育に見られるヘテローシスは開花時期とは独立である
    川辺隆大, Dennis ES, 藤本龍
    日本育種学会, Sep. 2011
    Poster presentation

  • シロイヌナズナとハクサイにおいて、生育初期のシュートでヘテローシスが見られる
    藤本龍, 川辺隆大, 今野周平, 加治誠, ジム ピーコック, エリザベス デニス
    日本育種学会, Sep. 2011
    Oral presentation

  • Epigenetic and genetic variation in imprinted genes between Arabidopsis thaliana and Arabidopsis lyrata
    Fujimoto, R, Sasaki, T, Dennis, E.S
    XVIII International botanical congress, Jul. 2011, English
    Oral presentation

  • Arabidopsis属の雑種強勢が見られる2つの人工複二倍体間でのゲノムワイドな転写の比較
    川辺隆大, 佐々木卓, Elizabeth S. Dennis, 藤本龍
    植物生理学会, Mar. 2011
    Poster presentation

  • Epigenetic variation in the FWA gene within the genus Arabidopsis
    藤本龍, Elizabeth S. Dennis
    日本植物生理学会, Mar. 2011
    Poster presentation

  • The whole genome transcriptome suggests early events are associated with heteosis in Arabidopsis thaliana
    Fujimoto R, Taylor JM, Shirasawa S, Peacock WJ, Dennis ES
    Combio2011, 2011, English
    Oral presentation

  • Early developmental heterosis in both Arabidopsis thaliana and Chinese cabbage
    Fujimoto Ryo, Kawanabe Takahiro
    Epigenetics and heterosis meeting, 2011, English
    Oral presentation

  • Arabidopsis属の人工複二倍体に見られる非相加的遺伝子制御と雑種強勢様の表現型
    藤本龍, 川辺隆大, 佐々木卓, Elizabeth S. Dennis
    日本育種学会, Sep. 2010
    Poster presentation

  • Diversity of epigenetic control in the FWA gene within the genus Arabidopsis
    Fujimoto, R, Kudoh, H, Dennis, E.S, Kakutani, T
    21st International Conference on Arabidopsis Research, Jun. 2010, English
    Poster presentation

  • Genome wide gene expression in artificially synthesized amphidiploids in Arabidopsis
    Fujimoto, R, Taylor, J.M, Kakutani, T, Dennis, E.S
    Combio 2009, Dec. 2009, English
    Poster presentation

  • Control of FWA gene silencing in the genus Arabidopsis
    Fujimoto, R, Kudoh, H, Dennis, E.S, Kakutani, T
    Epigenetics 2009, Dec. 2009, English
    Poster presentation

  • Heterosis at early developmental stages in Arabidopsis thaliana is independent of flowering time
    Fujimoto, R, Scofield G. N, Peacock W. J, Dennis, E.S
    Combio 2009, Dec. 2009, English
    Oral presentation

  • Evolution and metastable epigenetic states of imprinted FWA genes in Arabidopsis
    Fujimoto Ryo
    Epigenome Conference, Sep. 2008, English
    Oral presentation

  • Intra-specific variation of vegetative expression of imprinted FWA gene
    Fujimoto, R, Kudoh, H, Kakutani, T
    19th International conference on Arabidopsis research, Jul. 2008, English
    Poster presentation

  • Evolution of imprinted FWA genes in Arabidopsis
    Fujimoto, R, Kinoshita, Y, Kawabe, A, Kinoshita, T, Takashima, K, Nordborg, M, Nasrallah, M. E. Shimizu, K. K, Kudoh, H, Kakutani, T
    XX International Congress of Genetics, Jul. 2008, English
    Poster presentation

  • 栄養組織におけるインプリント遺伝子FWAの発現の同一種内での多様性
    藤本龍, 工藤洋, 角谷徹仁
    第2回日本エピジェネティクス研究会年会, May 2008
    Poster presentation

  • Evolution and control of imprinted FWA genes in Arabidopsis
    Fujimoto, R, Kinoshita, Y, Kawabe, A, Kinoshita, T, Takashima, K, Nordborg, M, Nasrallah, M. E. Shimizu, K. K, Kudoh, H, Kakutani, T
    NIBB-EMBL Joint Meeting: Evolution of epigenetic regulation, Mar. 2008, English
    Poster presentation

  • Brassica rapaにおけるBrDDM1によるDNAメチル化制御の解析
    佐々木卓, 藤本龍, 岸谷幸枝, 西尾剛
    日本育種学会, Mar. 2008
    Oral presentation

  • インプリント遺伝子FWAの構造と発現制御の進化
    藤本龍
    特定領域研究「植物の生殖過程におけるゲノム障壁」若手研究者ワークショップ, Nov. 2007
    Public symposium

  • Brassica rapaにおける器官特異的DNAメチル化の解析
    佐々木卓, 藤本龍, 岸谷幸枝, 西尾剛
    日本育種学会, Sep. 2007
    Poster presentation

  • Brassica属自家不和合性複合遺伝子座における組換えの検出と塩基配列多型への影響
    宅野将平, 藤本龍, 杉村哲, 西尾剛
    日本育種学会, Sep. 2007
    Oral presentation

  • シロイヌナズナのインプリント遺伝子FWAの進化
    藤本龍, 河邊昭, 木下由紀, 高嶋和也, 木下哲, 三浦明日香, 角谷徹仁
    日本遺伝学会, Sep. 2007
    Oral presentation

  • インプリント遺伝子FWAの構造と発現制御の進化：反復配列の役割について
    藤本龍, 木下由紀, 高嶋和也, 木下哲, 三浦明日香, 角谷徹仁
    第1回日本エピジェネティクス研究会年会, Jun. 2007
    Poster presentation

  • Suppression of gene expression of a recessive SP11/SCR allele by an untranscribed SP11/SCR allele on same and different chromosomes in Brassica self-incompatibility
    Fujimoto, R, Okamoto, S, Sasaki, T, Sugimura, T, Nishio, T
    The Fifth Okazaki Biology Conference: Speciation and Adaptation, Mar. 2007, English
    Poster presentation

  • Brassica rapaにおけるDDM1のDNAメチル化ターゲットの解析
    佐々木卓, 藤本龍, 西尾剛
    日本植物生理学会, Mar. 2007
    Poster presentation

  • Brassica rapaでの器官特異的な内生DNAメチル化制御と、BrDDM1によるDNAメチル化制御の解析
    佐々木卓, 藤本龍, 西尾剛
    2006年度遺伝研研究集会「高等植物の生殖形質におけるゲノム障壁制御遺伝子の分子遺伝学的解析」/特定領域研究「植物ゲノム障壁」ワークショップ, Nov. 2006
    Poster presentation

  • アブラナ科植物の自家和合性変異体の和合性変異の解析
    藤本龍, 杉村哲, 西尾剛
    2006年度遺伝研研究集会「高等植物の生殖形質におけるゲノム障壁制御遺伝子の分子遺伝学的解析」/特定領域研究「植物ゲノム障壁」ワークショップ, Nov. 2006
    Poster presentation

  • Brassica rapa の自家和合性品種Yellow sarsonの起源Sハプロタイプの同定とS遺伝子座のゲノム構造の変異
    日本育種学会
    Sep. 2006
    Poster presentation

  • アブラナ科植物種におけるレトロトランスポゾンの系統解析
    宅野将平, 藤本龍, 西尾剛
    日本育種学会, Sep. 2006
    Poster presentation

  • Brassica rapaのddm1-RNAi形質転換体における低メチル化の遺伝について
    藤本龍, 佐々木卓, 品田智隆, 西尾剛
    日本育種学会, Sep. 2006
    Poster presentation

  • Brassica rapaにおけるddm1 RNAi形質転換体の作出とDDM1のメチル化ターゲットの解析
    佐々木卓, 藤本龍, 岸谷幸枝, 西尾剛
    日本育種学会, Sep. 2006
    Oral presentation

  • An alloplasmic male-sterile line of Brassica oleracea harboring the mitochondria from Diplotaxis muralis expresses a novel chimeric open reading frame, orf72
    Shinada, T, Kikuchi, Y, Fujimoto, R, Kishitani, S
    XIX th International Congress on Sexual Plant Reproduction, Jul. 2006, English
    Poster presentation

  • Suppression of gene expression of a recessive SP11/SCR allele by an untranscribed SP11/SCR allele in Brassica self-incompatibility
    Fujimoto, R, Sugimura, T, Shinada, T, Nishio, T
    XIX th International Congress on Sexual Plant Reproduction, Jul. 2006, English
    Poster presentation

  • Brassica rapaにおけるDNAメチルトランスフェラーゼ遺伝子の解析
    藤本龍, 佐々木卓, 西尾剛
    日本植物生理学会, Mar. 2006
    Poster presentation

  • Brassica rapaにおける低メチル化形質転換体の解析
    藤本龍, 杉村哲, 西尾剛
    日本分子生物学会, Dec. 2005
    Poster presentation

  • アブラナ科F1品種の自殖分離集団における自家不和性強度の評価
    杉村哲, 藤本龍, 西尾剛
    岩手大学-東北大学ジョイント国際シンポジウム, Aug. 2005
    Poster presentation

  • SLGからSRKへのgene conversionによって生じた自家和合性の解析
    藤本龍, 杉村哲, 西尾剛
    日本育種学会, Aug. 2005
    Poster presentation

  • Self-compatibility caused by a gene conversion between SRK and SLG of the same S haplotype
    Fujimoto, R, Sugimura, T, Nishio, T
    Plant Biology, Jul. 2005, English
    Poster presentation

  • アブラナ科自家和合性系統の和合性突然変異の解明
    藤本龍, 品田智隆, 岡本俊介, 西尾剛
    日本植物生理学会, Mar. 2005
    Poster presentation

  • 自家和合性Brassica rapa L. var yellow sarsonのSRK、SP11の遺伝子構造
    藤本龍
    2004年度遺伝研研究集会「高等植物の生殖システム統御機能の分子遺伝学的解析」, Nov. 2004
    Public symposium

  • Gene structure of SRK and SP11/SCR in self-compatible B. rapa L. var. yellow sarson
    Fujimoto, R, Sawasato, A, Fukai, E, Nishio, T
    Joint Meeting of the 14th Crucifer Genetics Workshop and the 4th ISHS Symposium on Brassica., Oct. 2004, English
    Poster presentation

  • 自家不和合性の認識特異性とS遺伝子座の進化
    西尾剛, 佐藤豊, 藤本龍, 岡本俊介
    日本遺伝学会, Sep. 2004
    Nominated symposium

  • Brassica rapa種内においてSRK、SLGが類似するSハプロタイプのゲノム構造の比較
    藤本龍, 岡本俊介, 西尾剛
    日本育種学会, Sep. 2004
    Oral presentation

  • Comparison of the genome structure of the self-incompatibility (S) locus in interspecific pairs of S haplotypes
    Fujimoto, R, Okazaki, K, Kusaba, M, Nishio, T
    XVIIIth International Congress on Sexual Plant Reproduction, Aug. 2004, English
    Poster presentation

  • Diversification and evolution of S haplotypes revealed by the study of Brassica and Rhaphanus self-incompatibility
    Nishio, T, Sato, Y, Fujimoto, R, Okamoto, S
    XVIIIth International Congress on Sexual Plant Reproduction, Aug. 2004, English
    [Invited]
    Invited oral presentation

  • Brassica oleraceaとBrassica rapaの自家不和合性クラスIIS遺伝子座のゲノム構造の比較
    藤本龍, 岡本俊介, 岡崎桂一, 西尾剛
    日本育種学会, Apr. 2004
    Oral presentation

  • Commonality of self-recognition specificity of S haplotypes between Brassica oleracea and Brassica rapa
    Sato, Y, Fujimoto, R, Nishio, T
    International symposium on plant self-incompatibility, Sep. 2003, English
    Poster presentation

  • Genomic organization of the S core region and the S flanking regions of a class-II S haplotype in Brassica rapa
    Fukai, E, Fujimoto R, Nishio, T
    International symposium on plant self-incompatibility, Sep. 2003, English
    Poster presentation

  • Comparison of self-incompatibility (S ) locus between Brassica oleracea and Brassica rapa, and diversity of the retrotransposons found in the S locus region of Brassica oleracea
    Fujimoto, R, Kusaba, M, Nishio, T
    International symposium on plant self-incompatibility, Sep. 2003, English
    Poster presentation

  • Brassica oleraceaとBrassica rapaの自家不和合性(S)遺伝子座の比較、及びBrassica oleraceaに存在するレトロトランスポゾンの多様性
    藤本龍, 西尾剛
    Sep. 2003
    Oral presentation

  • Genomic organization of the S core region and the S flanking regions of a class-II S haplotype in Brassica rapa
    Fukai, E, Fujimoto, R, Nishio, T
    Plant Biology, Jul. 2003, English
    Poster presentation

  • Comparison of genome structure of classⅠ self-incompatibility (S) locus between Brassica oleracea and Brassica rapa
    Fujimoto, R, Sato Y, Kusaba M, Nishio, T
    Plant Biology, Jul. 2003, English
    Poster presentation

  • Brassica oleraceaとB. rapaのSハプロタイプが有する自己認識特異性の共通性
    佐藤豊, 藤本龍, 鳥山欽哉, 西尾剛
    日本育種学会, Apr. 2003
    Oral presentation

  • Brassica rapa自家不和合性クラスIIS遺伝子座のゲノム構造解析
    深井英吾, 藤本龍, 西尾剛
    日本育種学会, Sep. 2002
    Oral presentation

  • Brassica oleracea及びBrassica rapaの自家不和合性クラスIS遺伝子座のゲノム構造の比較
    藤本龍, 西尾剛
    日本育種学会, Sep. 2002
    Oral presentation

  • SP11を用いたドットブロット分析によるSハプロタイプ判定
    藤本龍, 西尾剛
    日本育種学会, Mar. 2002
    Oral presentation

■ Research Themes
  • Elucidation of molecular mechanism of chiasma localization in bunching onion and its application to bulb onion breeding
    執行 正義, 佐藤 修正, 藤本 龍, 石井 孝佳
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Yamaguchi University, 01 Apr. 2024 - 31 Mar. 2027

  • アブラナ科野菜の雑種強勢における顕性遺伝子座の役割の解明
    藤本 龍, 奥崎 文子, 藤井 壮太
    日本学術振興会, 科学研究費助成事業, 基盤研究(B), 神戸大学, 01 Apr. 2022 - 31 Mar. 2026

  • 藤本 龍
    科学技術振興機構, 産学が連携した研究開発成果の展開 研究成果展開事業 研究成果最適展開支援プログラム(A-STEP) 産学共同(育成型), 神戸大学, 2023 - 2025
    アブラナ科葉・根菜類では、栽培中に低温にさらされると、花成誘導・とう立ち(抽だい)が起こり、食味等の品質が低下するため、栽培中に低温にさらされても花成誘導されにくい晩抽性に優れた品種が望まれている。しかし、晩抽性の能力を高めると花を咲かせるのに必要な低温処理期間が長くなることから、品種育成の効率化を妨げるといったジレンマが存在する。そこで本研究では、遺伝子修飾を巧みに利用することで、晩抽性を自在に制御する技術の確立を目指す。これにより、従来法とは異なった育種システムの構築が期待でき、育種法の幅を広げることが期待できる。本技術シーズを元に、産学連携へと発展させ、品種育成への導入を目指す。

  • ブラシカ属作物の春化機構における種内変異，種間変異の遺伝解析とエピゲノム解析
    岡崎 桂一, 深井 英吾, 藤本 龍, 板橋 悦子
    日本学術振興会, 科学研究費助成事業, 基盤研究(B), 新潟大学, 01 Apr. 2021 - 31 Mar. 2024

  • Comprehensive analysis SFBB for S-RNase in self-incompatibility of Japanese pear
    安田 剛志, 井上 加奈子, 藤本 龍
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2019 - 31 Mar. 2023
    ナシ属の自家不和合性による花粉管の伸長抑制は雌ずい側因子S-RNaseが花粉管内のRNAを分解することで起こる。一方, 和合花粉管内では花粉側因子であるF-boxタンパク質群（SFBB）によりS-RNaseが無毒化されると考えられている。本研究では, SハプロタイプにコードされるSFBB群を掌握することで, S-RNaseに対する特異性を推定する。その推定をペチュニアの花粉で発現させたSFBB融合タンパク質のS-RNaseに対する結合特性を検証することを目的とした。 令和元年度は, ニホンナシのS2, S3, S4 BACコンティグ内外に存在する未解読BACクローンを次世代シークエンサーPacBioRSIIで解析し, 取得した配列を既知のコンティグに統合した。コンティグ配列はS2-RNase上流578kb~下流481kb, S3-RNase上流824kb~下流471kb, S4-RNase上流935kb~下流538kbまで拡張され, それぞれ18個, 19個, 20個のSFBBが同定された。各コンティグの両末端にはSFBB様配列は見出せなかったことから, これらSハプロタイプがコードするSFBB群を掌握できた。RNA-seqデータから構築したS1,S5-SFBB群配列の非翻訳領域から設計したプライマーセットを用いたRT-PCRにより未だクローニングされていないSFBB群配列をクローニングし, 配列を確認した。一方で, Skを有するニホンナシ品種 ‘巾着’の花粉をRNA-seq解析して, Sk-SFBB群配列を構築した。 S4-SFBB4d1およびその対立遺伝子のS1-SFBB4d1をそれぞれ導入したペチュニア形質転換を作出し, RT-PCRにより花粉での発現を確認した。 電子線トモグラフィー解析に向けた技術的な課題（試料作製法および連続傾斜撮影法など）を洗い出した。

  • A challenge to modify the vernalization requirement of root and leaf vegetables of Brassica
    Fujimoto Ryo
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), Kobe University, 30 Jul. 2020 - 31 Mar. 2022
    This study aims to artificially control the low-temperature requirement of Brassica vegetables. In this study, we found four extremely late-bolting lines, one of which had an insertion in the first intron of the BrFLC2 or BrFLC3 gene. These inserted regions were highly DNA methylated. We suggest that DNA methylation in the inserted regions of these two genes are the possible cause of the decreased cold response. We also constructed a vector for epigenome editing using Arabidopsis thaliana to modify histone modifications at the FLC locus.

  • Elucidation of the molecular mechanism of hybrid vigor in Brassica vegetables
    FUJIMOTO RYO
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2019 - 31 Mar. 2022
    To elucidate the molecular mechanism of hybrid vigor in Chinese cabbage, we performed genetic, genome-wide association, and whole genome DNA methylation analyses. In the genetic analysis, we identified QTLs that are related leaf area at 14 days after sowing or yield. Whole genome DNA methylation analysis identified regions where DNA methylation levels differed between the parental lines and their F1 progenies. For genome-wide association analysis, RNA-seq was performed on 73 F1 hybrid cultivars to obtain polymorphic information. In addition, mutants of genes involved in DNA methylation were generated by genome editing.

  • Study on vernalization mechanism of Brassica crops using newly developed Intromap, a bioinformatic tool to identify alien genomic introgression regions
    Okazaki Keiichi
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Niigata University, 01 Apr. 2018 - 31 Mar. 2021
    In order to elucidate the vernalization mechanism of the genus Brassica, we did the following studies. We produced C-genome chromosome addition lines using BoFLC2-introgressed B. rapa as a recurrent parent and a cabbage as a c-genome chromosome donor, and characterized those lines with respect to the parental cultivars. In Chinese cabbage, the epigenetic regulation of gene expression at the FLC locus was clarified by RNA-seq, lncRNA analysis, and chromatin immunoprecipitation analysis. In addition, transcriptome analysis was performed on the leaves of the cabbage DH strain with/without low temperature treatment. QTL analysis was performed to determine vernalization characteristics of rapeseed cultivars using F2 populations derived from the crossing between seed vernalization typed rapeseed x green plant vernalization typed rapeseed.

  • 藤本 龍
    学術研究助成基金助成金／国際共同研究加速基金(国際共同研究強化), Apr. 2017 - Mar. 2020, Principal investigator
    Competitive research funding

  • 安田 剛志
    科学研究費補助金／基盤研究(B), Apr. 2015 - Mar. 2019
    Competitive research funding

  • 藤本 龍
    科学研究費補助金／若手研究(A), Apr. 2015 - Mar. 2019, Principal investigator
    Competitive research funding

  • Study on the mechanism of seed- and green plant-vernalization and development of green plant-vernalization type Chinese cabbage
    Keiichi Okazaki
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Niigata University, 01 Apr. 2015 - 31 Mar. 2018
    We characterized vernalization requirement of 49 strains of oilseed rape, and developed the F2 population of Isuzu-natane (low vernalization requirement) × synthetic B. napus (strong vernalization requirement ). In addition, we developed a line that was introduced the region containing BoFLC 2 region derived from cabbage into the top of chromosome 2 of Chinese cabbage. We showed that the differences of expression level of BrFLC 1/2/3 in cabbage and Chinese cabbage may be related to the diversity of vernalization requirement in cabbage and Chinese cabbage cultivars. The RNA-seq analysis revealed that long non coding RNA was expressed on BoFLC2 locus by low temperature treatment. Our developed Chinese cabbage line showed delay of flowering in the winter cropping pattern, compared with ordinary Chinese cabbage cultivars, indicating that it can be used as a breeding material.

  • アブラナ科植物におけるDNAメチル化による雑種強勢発現機構の解明
    藤本 龍
    独立行政法人日本学術振興会, 二国間国際交流（オーストラリア(OP)との共同研究）, 2017, Principal investigator
    Competitive research funding

  • アブラナ科野菜の白さび病抵抗性遺伝子の同定
    藤本 龍
    研究成果展開事業（マッチングプランナー プログラム）, 2016, Principal investigator
    Competitive research funding

  • アブラナ科植物のヘテローシスの分子機構の解析
    藤本 龍
    独立行政法人日本学術振興会, 二国間交流事業（オープンパートナーシップ共同研究）, 2016, Principal investigator
    Competitive research funding

  • 二国間交流「アブラナ科植物のヘテローシスの分子機構の解析」
    藤本 龍
    日本学術振興会, 二国間交流事業（オープンパートナーシップ共同研究）, 2015, Principal investigator
    Competitive research funding

  • さきがけ「雑種強勢の分子機構の解明とその高バイオマス作物への活用」
    藤本 龍
    科学技術振興機構, 戦略的創造研究推進事業 個人型研究さきがけ, 2015, Principal investigator
    Competitive research funding

  • 藤本 龍
    学術研究助成基金助成金／若手研究(B), Apr. 2012 - Mar. 2014, Principal investigator
    Competitive research funding

  • 藤本 龍
    科学研究費補助金／新学術領域研究, Apr. 2012 - Mar. 2014, Principal investigator
    Competitive research funding

  • 二国間交流「アブラナ科植物のヘテローシスの分子機構の解析」
    藤本 龍
    二国間交流事業（オープンパートナーシップ共同研究）, 2014, Principal investigator
    Competitive research funding

  • さきがけ「雑種強勢の分子機構の解明とその高バイオマス作物への活用」
    藤本 龍
    戦略的創造研究推進事業 個人型研究さきがけ, 2014, Principal investigator
    Competitive research funding

  • さきがけ「雑種強勢の分子機構の解明とその高バイオマス作物への活用」
    藤本 龍
    戦略的創造研究推進事業 個人型研究さきがけ, 2013, Principal investigator
    Competitive research funding

  • アブラナ科植物の自家不和合性遺伝子座に存在するレトロトランスポゾンの発現解析
    藤本 龍
    日本学術振興会, 科学研究費助成事業, 特別研究員奨励費, 東北大学, 2003 - 2005
    アブラナ科植物の自家不和合性は一つの遺伝子座(S)を想定することで説明されている。自家不和合性反応はS遺伝子座に遺伝子が座乗する柱頭側の認識分子SRKと花粉側の認識分子SP11の相互作用による。同じくS遺伝子座にあるSLGは、SRKの細胞外ドメインと相同性が見られる。F_1品種‘CR清雅65'(S-46/S-54)の自殖分離集団の中から、5つの自家和合性個体(S-54ホモ)が得られた。S-54の自家和合個体と自家不和合個体の相互交配の結果、和合性の原因が柱頭側にあることが明らかとなった。連鎖解析から和合性の要因がSと連鎖していることが明らかになった。柱頭側の認識因子であるSRKの発現量に差は見られなかったが、SLGからSRKへのgene conversionが生じていた。以上の結果から、自家和合性の原因は、gene conversionによって生じたSRKがSP11を認識できなくなったためであると考えた。 アブラナ科植物から、DNAのメチル化に関与すると考えられる遺伝子、BrMET1a、BrMET1b、BrCMT、BrDDM1を単離した。このうち、BrDDM1の配列を元にRNAiのコンストラクトを作成し、形質転換体の作成を行い、11個の独立した形質転換体を得た。形質転換体ではメチル化の程度の低下が確認され、その程度は個体間で異なった。メチル化の低下の程度が1番大きかった形質転換体の表現型はWTと変わらなかったが、WTで発現しないことを確認しているレトロトランスポゾンの発現が誘導されていた。このレトロトランスポゾンのメチル化程度を形質転換体とWTで比較したところ、形質転換体でDNAのメチル化の程度が低下していたことから、このレトロトランスポゾンの発現誘導は、メチル化レベルの低下により起こったと考えられた。

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