Research activity information

• Sep. 2021 日本畜産学会, 2021年度日本畜産学会賞, 兵庫県黒毛和種集団における肉質関連遺伝子の同定

  笹崎晋史


• Mar. 2011 日本畜産学会, 日本畜産学会奨励賞, 牛肉の品種及び産地を識別するDNAマーカーの開発

  SASAZAKI SHINJI

  Japan society

• Maternal and paternal lineage analysis of Island Southeast Asian goats reveals continental propagation routes and introgression through the Indian ocean

  Ryo Masuko, Ayin, Maho Masaoka, Fuki Kawaguchi, Shinji Sasazaki, Muhammad I. A. Dagong, Sri R. A. Bugiwati, Joseph S. Masangkay, Takahiro Yonezawa, Hideyuki Mannen

  Springer Science and Business Media LLC, Mar. 2025, Scientific Reports, 15(1) (1)

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Maternal genetic phylogeography analysis of Kyrgyz native cattle

  Ayin, Ryo Masuko, Naho Kakutani, Fuki Kawaguchi, Shinji Sasazaki, Sanjar Sultankulov, Asankadyr Zhunushov, Masahide Nishibori, Takahiro Yonezawa, Hideyuki Mannen

  Sep. 2024, Animal Science Journal, 95, e13999, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal


• Comprehensive genotyping analysis of single nucleotide polymorphisms responsible for beef marbling in Japanese Black cattle

  Shinji Sasazaki, Hina Kondo, Yurika Moriishi, Fuki Kawaguchi, Kenji Oyama, Hideyuki Mannen

  Abstract Background Beef marbling is considered a desirable trait in the meat industry. Therefore, understanding the genetic factors that cause marbling is important. Previously, we performed a genome-wide association study to examine genetic factors associated with beef marbling in Japanese Black cattle and identified a candidate region between 10–30 Mbp on chromosome 7. We verified the effect of the SNPs in this region on beef marbling using linkage disequilibrium block analysis. We narrowed down the candidate region to a range of 15.8–16.1 Mbp. In this study, we comprehensively detected all of the SNPs in this region and verified their effects on beef marbling. Results Genome resequencing using four animals exhibiting high beef marbling standard (BMS) and four with low BMS revealed a total of 1,846 polymorphisms within the candidate region. Based on the annotation, we selected 13 SNPs exhibiting a moderate impact, as no high-impact SNPs were detected. All of the SNPs represented missense polymorphisms and were located in the following seven genes: RDH8, ANGPTL6, DNMT1, MRPL4, ICAM1, ICAM3, and ICAM5. Finally, we determined the effects of these SNPs on the BMS of a Japanese Black cattle population (n = 529). Analysis of variance revealed that the five SNPs were located in genes encoding the intercellular adhesion molecules (ICAM1, ICAM3, and ICAM5), and showed a highly significant association compared with the remainder (p < 0.01). The lowest p-value was observed for ICAM3_c.739G > A (p = 1.18E-04). Previous studies have suggested that intercellular adhesion molecules (ICAM) may be an upstream factor that regulates adipocyte differentiation. Therefore, considering the polymorphism and putative gene function, we suggest that ICAM1 is potentially responsible for beef marbling. c.470C > G and/or c.994G > A on ICAM1 may be responsible for this quantitative trait locus. Conclusions Promising SNP candidates responsible for beef marbling were identified using extensive polymorphism verification in a previously reported QTL region. We aim to elucidate the mechanism of beef marbling in future studies by investigating how these polymorphisms alter protein structure and function.

  Springer Science and Business Media LLC, Feb. 2024, BMC Genomic Data, 25(1) (1), 17, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Comprehensive assessment of genetic diversity, structure, and relationship in four Japanese cattle breeds by 50K SNP array analysis

  Fuki KAWAGUCHI, Mitsuki NAKAMURA, Keiichi SUZUKI, Eiji KOBAYASHI, Shinji SASAZAKI, Hideyuki MANNEN

  Wiley, Oct. 2022, Animal Science Journal, 93(1) (1), e13770, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Application of Mass Spectrometry for Determining the Geographic Production Area of Wagyu Beef

  Shuji Ueda, Yasuharu Takashima, Yunosuke Gotou, Ryo Sasaki, Rio Nakabayashi, Takeshi Suzuki, Shinji Sasazaki, Ituko Fukuda, Biniam Kebede, Yuki Kadowaki, Maiko Tamura, Hiroki Nakanishi, Yasuhito Shirai

  Japanese Black cattle (Japanese Wagyu) beef is attracting attention for its aroma and marbling, and its handling is increasing worldwide. Here, we focused on the origin discrimination of Wagyu beef and analyzed the nutritional components of Japanese Wagyu (produced in multiple prefectures of Japan), Hybrid Wagyu (a cross between Angus and Wagyu cattle born in Australia and transported to Japan), and Australian Wagyu beef using mass spectrometry (MS). Triple-quadrupole liquid chromatography-MS was used to clarify the molecular species of lipids in Wagyu beef. Fourteen classes of lipids were separated, and 128 different triacylglycerides (TGs) were detected. A simple comparative analysis of these TGs using high-performance liquid chromatography revealed significantly higher levels of triolein (C18:1/C18:1/C18:1; abbreviated OOO) and C18:1/C18:1/C16:1 (OOPo) in Japanese Wagyu. Wagyu elements beef were comprehensively analyzed using inductively coupled plasma (ICP)-MS and ICP-optical emission spectrometry. We found significant differences in the rubidium, cesium, and lithium levels of Japanese and Australian Wagyu beef. On comparing metabolites using gas chromatography-MS, we identified significant differences in the levels of amino acids and other components of the Japanese and Australian Wagyu beef. These results suggest the possibility of determining the origin of Wagyu cattle breeds using MS and genetic discrimination.

  Aug. 2022, Metabolites, 12(9) (9), 777, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Verification of Candidate SNP Effects Reveals Two QTLs on BTA7 for Beef Marbling in Two Japanese Black Cattle Populations

  Shinji Sasazaki, Raito Yamamoto, Shintaro Toyomoto, Hina Kondo, Takayuki Akiyama, Namiko Kohama, Emi Yoshida, Fuki Kawaguchi, Kenji Oyama, Hideyuki Mannen

  In our previous study, we used genome resequencing to detect all candidate polymorphisms within a quantitative trait loci (QTL) region for beef marbling reported previously at 10–30 Mbp on bovine chromosome 7, and we selected 6044 polymorphisms as candidate quantitative trait nucleotides (QTNs). In the present study, we aimed to identify quantitative trait genes (QTGs) and QTNs in this QTL region by verifying the effect of SNPs on beef marbling in two Japanese Black cattle populations using a Dynamic Array integrated fluidic circuit. In total, 96 selected SNPs were genotyped in 441 and 529 animals in Hyogo and Miyazaki cattle populations, respectively. The most significant p-values were detected in a SNP in a splice region of ALDH7A1 (SNP93_ALDH7A1; p = 3.46 × 10−5) in Hyogo cattle and a missense polymorphism of intercellular adhesion molecule-1 (ICAM1) (SNP37_ICAM1; p = 3.33 × 10−4) in Miyazaki cattle. Interestingly, SNP93_ALDH7A1 was not significant (p = 0.459) in Miyazaki cattle, and SNP37_ICAM1 showed a weakly significant association (p = 0.043) in Hyogo cattle. Thus, each population would likely have different QTGs and QTNs for beef marbling in the QTL region. In the Hyogo population, it was not possible to determine the accurate range of the linkage disequilibrium (LD) block in LD block analysis because of a strong LD structure throughout the assessed region. In Miyazaki cattle, however, an LD block containing SNP37_ICAM1 had a range of 15.8–16.1 Mbp, suggesting that QTNs would be located within this region. The functions of 19 genes in the LD block were investigated. ICAM1 is known to play an important role in adipocyte differentiation; given this function and the effect of amino acid substitution, SNP37_ICAM1 was identified as a promising candidate QTN for beef marbling. Further research on the effect of SNP37_ICAM1 on adipocyte differentiation is expected to provide insights into the mechanism underlying beef marbling formation.

  MDPI AG, Jul. 2022, Genes, 13(7) (7), 1190 - 1190, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Geographical contrasts of Y‐chromosomal haplogroups from wild and domestic goats reveal ancient migrations and recent introgressions

  Var Goats Consortium, Isaäc J. Nijman, Benjamin D. Rosen, Philippe Bardou, Thomas Faraut, Tristan Cumer, Kevin G. Daly, Zhuqing Zheng, Yudong Cai, Hojjat Asadollahpour, Bengi Çınar Kul, Wei‐Yi Zhang, E. Guangxin, A. Ayin, Hayley Baird, Meirat Bakhtin, Valentin A. Bâlteanu, Diana Barfield, Beate Berger, Thor Blichfeldt, Geert Boink, Sri R. A. Bugiwati, Zexi Cai, Sean Carolan, Emily Clark, Vlatka Cubric‐Curik, Muhammad I. A. Dagong, Tashi Dorji, Louise Drew, Jiazhong Guo, Jón Hallsson, Simon Horvat, Juha Kantanen, Fuki Kawaguchi, Polat Kazymbet, Negar Khayatzadeh, Namshin Kim, Manoj Kumar Shah, Yuying Liao, Amparo Martínez, Joseph S. Masangkay, Maho Masaoka, Raffaele Mazza, John McEwan, Marco Milanesi, Md. Omar Faruque, Yuto Nomura, Nadjet‐Amina Ouchene‐Khelifi, Filipe Pereira, Goutam Sahana, Mazdak Salavati, Shinji Sasazaki, Anne Da Silva, Mojca Simčič, Johann Sölkner, Alison Sutherland, Johannes Tigchelaar, Hongpin Zhang, Econogene Consortium, Paolo Ajmone‐Marsan, Daniel G. Bradley, Licia Colli, Cord Drögemüller, Yu Jiang, Chuzhao Lei, Hideyuki Mannen, François Pompanon, Gwenola Tosser‐Klopp, Johannes A. Lenstra

  Wiley, Jun. 2022, Molecular Ecology, 31(16) (16), 4364 - 4380, English

  [Refereed]

  Scientific journal


• Phylogenomics and Spatiotemporal Dynamics of Bovine Leukemia Virus Focusing on Asian Native Cattle: Insights Into the Early Origin and Global Dissemination

  Kohei Nishikaku, Takahiro Yonezawa, Masahide Nishibori, Masashi Harada, Fuki Kawaguchi, Shinji Sasazaki, Yasushi Torii, Kazuhiko Imakawa, Kuniko Kawai, Jianquan Liu, Hideyuki Mannen, Tomoko Kobayashi

  Bovine leukemia virus (BLV), the causative agent of enzootic bovine leukosis, is currently one of the most important pathogens affecting the cattle industry worldwide. Determining where and in which host it originated, and how it dispersed across continents will provide valuable insights into its historical emergence as the cattle pathogen. Various species in the Bos genus were domesticated in Asia, where they also diversified. As native cattle (taurine cattle, zebu cattle, yak, and water buffalo) are indigenous and adapted to local environments, we hypothesized that Asian native cattle could have harbored BLV and, therefore, that they were important for virus emergence, maintenance, and spread. In this study, phylogeographic and ancestral trait analyses—including sequences obtained from Asian native cattle—were used to reconstruct the evolutionary history of BLV. It was shown that, since its probable emergence in Asia, the virus spread to South America and Europe via international trade of live cattle. It was inferred that zebu cattle were the hosts for the early origin of BLV, while taurine cattle played the significant role in the transmission worldwide. In addition, the results of positive selection analysis indicate that yak had a substantially minor role in the transmission of this virus. In this study, endogenous deltaretrovirus sequences in bats, collected in Asian countries, were also analyzed on whether these sequences were present in the bat genome. Endogenous deltaretrovirus sequences were detected from bat species endemic to specific regions and geographically isolated for a long time. Endogenous deltaretrovirus sequences from these geographically isolated species represent ancient exogenous deltaretroviruses distributions. The phylogenetic analysis revealed that these newly obtained endogenous deltaretrovirus sequences were closely related to those of BLV from Asian native cattle, indicating that BLV-related ancient deltaretroviruses circulated in Asia long before the emergence of BLV. Together, our analyses provide evidence for origin and spatiotemporal dynamics of BLV.

  Frontiers Media SA, Jun. 2022, Frontiers in Microbiology, 13, English

  [Refereed]

  Scientific journal


• Development of DNA markers for improvement of meat quality in a Japanese Black cattle population in Hyogo Prefecture

  Shinji Sasazaki

  Dec. 2021, Animal Science Journal, 92(1) (1), English

  [Refereed][Invited]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• FGF5 and EPAS1 gene polymorphisms are associated with high‐altitude adaptation in Nepalese goat breeds

  Shinji Sasazaki, Koichiro Tomita, Yuto Nomura, Fuki Kawaguchi, Tetsuo Kunieda, Manoj Kumar Shah, Hideyuki Mannen

  Lead, Wiley, Sep. 2021, Animal Science Journal, 92(1) (1), English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Recent achievements of candidate polymorphism detection for fatty acid composition in Japanese Black cattle

  Fuki KAWAGUCHI, Hideyuki MANNEN, Shinji SASAZAKI

  Corresponding, Aug. 2021, The Journal of Animal Genetics, 49, 67 - 75, English

  [Refereed]


• Effect of Five Polymorphisms on Percentage of Oleic Acid in Beef and Investigation of Linkage Disequilibrium to Confirm the Locations of Quantitative Trait Loci on BTA19 in Japanese Black Cattle

  Fuki Kawaguchi, Fuka Kakiuchi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Five polymorphisms associated with the percentage of oleic acid (C18:1) in beef fat were previously reported on bovine chromosome 19 in different Japanese Black cattle populations. This study aimed to verify the effects of these five polymorphisms on C18:1 using the same Japanese Black cattle population and conduct linkage disequilibrium (LD) analysis in order to determine the locations of the quantitative trait loci (QTLs). We genotyped the five polymorphisms (SREBP1 c.1065 + 83 (84bp indel), STARD3 c.1187 C > T, GH c.379 C > G, FASN g.841 G > C, and FASN g.16024 A > G) in two populations, which were bred in Hyogo and Gifu Prefectures, Japan (n = 441 and 443, respectively) in order to analyze their effects on C18:1 using analysis of variance (ANOVA). In the Hyogo population, SREBP1 c.1065 + 83 and STARD3 c.1187 C > T were significantly associated with C18:1 (p < 0.001). Meanwhile, FASN g.841 G > C, FASN g.16024 A > G, and GH c.379 C > G were significantly associated with C18:1 (p < 0.01) in the Gifu population. LD analysis was subsequently conducted to detect the range of the QTLs, which ranged from 32.2 to 46.4 Mbp and from 47.8 to 52.1 Mbp in the Hyogo and Gifu populations, respectively. In conclusion, this study confirmed the existence of QTLs on BTA19 and divided the candidate region for each QTL based on LD coefficients. These results could contribute to efficient searches for responsible genes and polymorphisms for fatty acid composition.

  Corresponding, MDPI AG, Jun. 2021, Life, 11(7) (7), 597 - 597, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Genetic characteristics of Korean Jeju Black cattle with high density single nucleotide polymorphisms

  M. Zahangir Alam, Yun-Mi Lee, Hyo-Jung Son, Lauren H. Hanna, David G. Riley, Hideyuki Mannen, Shinji Sasazaki, Se Pill Park, Jong-Joo Kim

  Objective: Conservation and genetic improvement of cattle breeds require information about genetic diversity and population structure of the cattle. In this study, we investigated the genetic diversity and population structure of the three cattle breeds in the Korean peninsula.Methods: Jeju Black, Hanwoo, Holstein cattle in Korea, together with six foreign breeds were examined. Genetic diversity within the cattle breeds was analyzed with minor allele frequency (MAF), observed and expected heterozygosity (HO and HE), inbreeding coefficient (FIS) and past effective population size. Molecular variance and population structure between the nine breeds were analyzed using a model-based clustering method. Genetic distances between breeds were evaluated with Nei’s genetic distance and Weir and Cockerham’s FST.Results: Our results revealed that Jeju Black cattle had lowest level of heterozygosity (HE = 0.21) among the studied taurine breeds, and an average MAF of 0.16. The level of inbreeding was –0.076 for Jeju Black, while –0.018 to –0.118 for the other breeds. Principle component analysis and neighbor-joining tree showed a clear separation of Jeju Black cattle from other local (Hanwoo and Japanese cattle) and taurine/indicine cattle breeds in evolutionary process, and a distinct pattern of admixture of Jeju Black cattle having no clustering with other studied populations. The FST value between Jeju Black cattle and Hanwoo was 0.106, which was lowest across the pair of breeds ranging from 0.161 to 0.274, indicating some degree of genetic closeness of Jeju Black cattle with Hanwoo. The past effective population size of Jeju Black cattle was very small, i.e. 38 in 13 generation ago, whereas 209 for Hanwoo.Conclusion: This study indicates genetic uniqueness of Jeju Black cattle. However, a small effective population size of Jeju Black cattle indicates the requirement for an implementation of a sustainable breeding policy to increase the population for genetic improvement and future conservation.

  Asian Australasian Association of Animal Production Societies, May 2021, Animal Bioscience, 34(5) (5), 789 - 800

  [Refereed]

  Scientific journal


• Indonesian native goats (Capra hircus) reveal highest genetic frequency of mitochondrial DNA haplogroup B in the world

  Hideyuki Mannen, Kenta Iso, Fuki Kawaguchi, Shinji Sasazaki, Takahiro Yonezawa, Muhammad Ihsan Andi Dagong, Sri Rachma Aprilita Bugiwati

  Nov. 2020, Animal Science Journal

  [Refereed]

  Link to Kobe Univ. Repository (Kernel)


• Cattle mitogenome variation reveals a post-glacial expansion of haplogroup P and an early incorporation into northeast Asian domestic herds

  Hideyuki Mannen, Takahiro Yonezawa, Kako Murata, Aoi Noda, Fuki Kawaguchi, Shinji Sasazaki, Anna Olivieri, Alessandro Achilli, Antonio Torroni

  Surveys of mitochondrial DNA (mtDNA) variation have shown that worldwide domestic cattle are characterized by just a few major haplogroups. Two, T and I, are common and characterize Bos taurus and Bos indicus, respectively, while the other three, P, Q and R, are rare and are found only in taurine breeds. Haplogroup P is typical of extinct European aurochs, while intriguingly modern P mtDNAs have only been found in northeast Asian cattle. These Asian P mtDNAs are extremely rare with the exception of the Japanese Shorthorn breed, where they reach a frequency of 45.9%. To shed light on the origin of this haplogroup in northeast Asian cattle, we completely sequenced 14 Japanese Shorthorn mitogenomes belonging to haplogroup P. Phylogenetic and Bayesian analyses revealed: (1) a post-glacial expansion of aurochs carrying haplogroup P from Europe to Asia; (2) that all Asian P mtDNAs belong to a single sub-haplogroup (P1a), so far never detected in either European or Asian aurochs remains, which was incorporated into domestic cattle of continental northeastern Asia possibly ~ 3700 years ago; and (3) that haplogroup P1a mtDNAs found in the Japanese Shorthorn breed probably reached Japan about 650 years ago from Mongolia/Russia, in agreement with historical evidence.

  Springer Science and Business Media LLC, Nov. 2020, Scientific Reports, 10(1) (1), English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effect of DNA markers on the fertility traits of Japanese Black cattle for improving beef quantity and quality

  Fuki Kawaguchi, Miyako Tsuchimura, Kenji Oyama, Tamako Matsuhashi, Shin Maruyama, Hideyuki Mannen, Shinji Sasazaki

  Abstract. Carcass traits have been efficiently improved by recent selection using DNA markers in beef cattle. Additionally, DNA markers might have an effect on other traits such as fertility traits; therefore attention should also be paid to such pleiotropic effects. However, the effects of the markers on both carcass and fertility traits have never been evaluated in the same population, since they are generally measured in different populations. The objective in the current study was to discuss effectiveness of DNA markers developed for carcass traits through investigation of their effects on carcass and fertility traits in a population. We genotyped six markers SCD V293A, FASN g.841G>C, PLAG1 g.49066C>G, NCAPG I442M, DGAT1 K232A, and EDG1 g.1471620G>T in a Japanese Black cattle population (n=515). To investigate their effects on carcass and fertility traits, we performed statistical analysis (ANOVA and the Tukey–Kramer honestly significant difference (HSD) test). In the results, three of six markers, SCD V293A, NCAPG I442M, and EGD1 g.1471620G>T, were significantly associated with both carcass and fertility traits. Remarkably, the same allele for each marker had positive effects on both traits, suggesting that we would be able to simultaneously improve them using these markers in this population. However, previous studies reported that the effects of DNA markers could differ among populations. Therefore, it is necessary to confirm the effect of the marker in each population before it is used for improvement.

  Corresponding, Copernicus GmbH, Jan. 2020, Archives Animal Breeding, 63(1) (1), 9 - 17, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Detection of candidate polymorphisms around the QTL for fat area ratio to rib eye area on BTA7 using whole‐genome resequencing in Japanese Black cattle

  Shinji Sasazaki, Fuki Kawaguchi, Ayaka Nakajima, Raito Yamamoto, Takayuki Akiyama, Namiko Kohama, Emi Yoshida, Eiji Kobayashi, Takeshi Honda, Kenji Oyama, Hideyuki Mannen

  Wiley, Jan. 2020, Animal Science Journal, 91(1) (1), e13335

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The non-synonymous mutation in SPP1 gene influences carcass weight in Japanese Black cattle

  Matsumoto H, Kohara R, Sugi M, Usui A, Oyama K, Mannen H, Sasazaki S

  Meat quality in beef cattle is controlled by genetic factors. SPP1 (secreted phosphoprotein 1) gene, coding a multifunctional cytokine with diverse biological functions, is the candidate gene influencing carcass traits. In this study, we tried to discover DNA polymorphisms associated with beef quality in bovine SPP1 gene, so that two SNPs (single nucleotide polymorphisms) in the promoter region and one SNP in the CDS (coding sequence) region were identified. Although the formers were predicted to alter SPP1 expression, they did not show any effects on the traits. On the contrary, statistical analysis revealed that g.58675C > T, a non-synonymous mutation from threonine to methionine in the conservative region, had a significant effect on carcass weight. Carcass weight of the animals with C/T allele (473.9 ± 6.0 kg) was significantly heavier than that of the C/C homozygotes (459.2 ± 2.8 kg). Because SPP1 gene functions in skeletal muscle cells as a positive regulator, the non-synonymous mutation might influence muscle development and remodeling, resulting in increased carcass weight of the C/T animals. Our results indicate that the SNP can be applied as a DNA marker for the improvement of beef cattle.

  Last, Dec. 2019, Heliyon, 5(12) (12), e03006, English, International magazine

  [Refereed]

  Scientific journal


• Phylogeographic Analysis of Madagascan Goats Using mtDNA Control Region and SRY Gene Sequences

  Risa Tabata, Fuki Kawaguchi, Shinji Sasazaki, Yoshio Yamamoto, Felix Rakotondraparany, Fanomezana Mihaja Ratsoavina, Takahiro Yonezawa, Hideyuki Mannen

  Zoological Society of Japan, Aug. 2019, Zoological Science, 36(4) (4), 294 - 294, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The SNPs in bovine MMP14 promoter influence on fat-related traits

  Hirokazu Matsumoto, Fuki Kawaguchi, Shotaro Itoh, Suzuna Yotsu, Kento Fukuda, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Last, Elsevier BV, Jun. 2019, Meta Gene, 20, 100558 - 100558

  [Refereed]

  Scientific journal


• Whole‐genome resequencing to identify candidate genes for the QTL for oleic acid percentage in Japanese Black cattle

  Fuki Kawaguchi, Hiroto Kigoshi, Moriyuki Fukushima, Eiji Iwamoto, Eiji Kobayashi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Corresponding, Wiley, Apr. 2019, Animal Science Journal, 90(4) (4), 467 - 472, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討

  山本 雷斗, 川口 芙岐, 秋山 敬孝, 吉田 恵実, 小浜 菜美子, 小林 栄治, 大山 憲二, 万年 英之, 笹崎 晋史

  (公社)日本畜産学会, Mar. 2019, 日本畜産学会大会講演要旨集, 125回, 177 - 177, Japanese


• The Eurasian Steppe is an important goat propagation route: A phylogeographic analysis using mitochondrial DNA and Y-chromosome sequences of Kazakhstani goats

  Risa Tabata, Fuki Kawaguchi, Shinji Sasazaki, Yoshio Yamamoto, Meirat Bakhtin, Polat Kazymbet, Alykhan Meldevekob, Maratbek Z. Suleimenov, Masahide Nishibori, Hideyuki Mannen

  Wiley, Mar. 2019, Animal Science Journal, 90(3) (3), 317 - 322, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effect of STARD3 gene polymorphism on carcass traits and fatty acid composition in Japanese Black cattle

  Hiroto Kigoshi, Fuki Kawaguchi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Corresponding, Japanese Society of Animal Breeding and Genetics, 2019, The Journal of Animal Genetics, 47(2) (2), 37 - 45

  [Refereed]

  Scientific journal


• Kazakhstani native cattle reveal highly divergent mtDNA from Bos taurus and Bos indicus lineages with an absence of Bos indicus Y chromosome

  Hayate Yamanaka, Kako Murata, Risa Tabata, Fuki Kawaguchi, Shinji Sasazaki, Yoshio Yamamoto, Meirat Bakhtin, Polat Kazymbet, Alykhan Meldevekob, Maratbek Z. Suleimenov, Masahide Nishibori, Hideyuki Mannen

  Wiley, Jan. 2019, Animal Science Journal, 90(1) (1), 29 - 34, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 日本ホルスタインにおいて観察された稀少なmtDNAハプログループP

  野田葵, 川口 芙岐, 笹崎晋史, 万年英之

  Aug. 2018, 動物遺伝育種研究, 46(2) (2), 49 - 55, Japanese

  [Refereed]

  Scientific journal


• Pool-based genome-wide association study identified novel candidate regions on BTA9 and 14 for oleic acid percentage in Japanese Black cattle

  Fuki Kawaguchi, Hiroto Kigoshi, Ayaka Nakajima, Yuta Matsumoto, Yoshinobu Uemoto, Moriyuki Fukushima, Emi Yoshida, Eiji Iwamoto, Takayuki Akiyama, Namiko Kohama, Eiji Kobayashi, Takeshi Honda, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Corresponding, Wiley, Aug. 2018, Animal Science Journal, 89(8) (8), 1060 - 1066, English

  [Refereed]

  Scientific journal


• Indelマーカーを用いた家畜ウシ11集団に対する遺伝的構造および系統解析

  山中 颯, 笹崎晋史, Hla Hla Moe, Moe Lwin, 下桐 猛, 万年英之

  Aug. 2018, 日本畜産学会報, 89(3) (3), 313 - 321, Japanese

  [Refereed]

  Scientific journal


• A genome-wide association study for fat-related traits computed by image analysis in Japanese Black cattle

  Ayaka Nakajima, Fuki Kawaguchi, Yoshinobu Uemoto, Moriyuki Fukushima, Emi Yoshida, Eiji Iwamoto, Takayuki Akiyama, Namiko Kohama, Eiji Kobayashi, Takeshi Honda, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Corresponding, Wiley, May 2018, Animal Science Journal, 89(5) (5), 743 - 751, English

  [Refereed]

  Scientific journal


• ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索

  川口 芙岐, 木越 洸利, 小浜 菜美子, 秋山 敬孝, 福島 護之, 吉田 恵実, 小林 栄治, 大山 憲二, 万年 英之, 笹崎 晋史

  (公社)日本畜産学会, Mar. 2018, 日本畜産学会大会講演要旨集, 124回, 111 - 111, Japanese


• The rare mtDNA haplogroup P observed in Japanese Holstein cattle

  Aoi NODA, Fuki KAWAGUCHI, Shinji SASAZAKI,, Hideyuki MANNEN

  Japanese Society of Animal Breeding and Genetics, 2018, The Journal of Animal Genetics, 46(2) (2), 49 - 55

  [Refereed]

  Scientific journal


• The mtDNA haplogroup P of modern Asian cattle: A genetic legacy of Asian aurochs?

  Aoi Noda, Riku Yonesaka, Shinji Sasazaki, Hideyuki Mannen

  Public Library of Science, Jan. 2018, PLoS ONE, 13(1) (1), e0190937, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Application of DNA markers for discrimination between Japanese and Australian Wagyu beef

  Fuki Kawaguchi, Yuto Kitamura, Ryuji Nakajima, Masayoshi Takahashi, Hiroaki Goto, Yuichi Washida, Yoshikazu Yamamoto, Shinji Sasazaki, Hideyuki Mannen

  Corresponding, Wiley, Jan. 2018, Animal Science Journal, 89(1) (1), 257 - 258, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Research Article Effect of the PLAG1 gene polymorphism on oleic acid percentage in Japanese Black cattle populations

  H. Kigoshi, F. Kawaguchi, R. Yasuzumi, K. Oyama, H. Mannen, S. Sasazaki

  Corresponding, Genetics and Molecular Research, 2018, Genetics and Molecular Research, 17(3) (3), 18056, English

  [Refereed]

  Scientific journal


• Preservation of Genetic Diversity of the Asian Native Goats

  Honda T, Shibano M, Matsumoto H, Sasazaki S, Oyama K, Mannen H

  Sep. 2017, Animal Veterinary Sciences, 5, 69 - 72, English

  [Refereed]

  Scientific journal


• Low mitochondrial DNA diversity of Japanese Polled and Kuchinoshima feral cattle

  Hideyuki Mannen, Riku Yonesaka, Aoi Noda, Takeshi Shimogiri, Ichiro Oshima, Kiyomi Katahira, Misao Kanemaki, Tetsuo Kunieda, Yousuke Inayoshi, Fumio Mukai, Shinji Sasazaki

  May 2017, ANIMAL SCIENCE JOURNAL, 88(5) (5), 739 - 744, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Identification of leptin gene polymorphisms associated with carcass traits and fatty acid composition in Japanese Black cattle

  Fuki Kawaguchi, Kazuki Okura, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Corresponding, Mar. 2017, ANIMAL SCIENCE JOURNAL, 88(3) (3), 433 - 438, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The effect of LPL gene polymorphisms on fatty acid composition in Japanese Black cattle

  Fuki KAWAGUCHI, Satoshi KOGA, Kenji OYAMA, Hideyuki MANNEN,, Shinji SASAZAKI

  Corresponding, Japanese Society of Animal Breeding and Genetics, 2017, The Journal of Animal Genetics, 45(1) (1), 3 - 8, Japanese

  [Refereed]

  Scientific journal


• 高密度SNPアレイを用いた口之島野生化牛の全常染色体及び各染色体に対する遺伝的多様性の評価

  齊藤祐也, 笹崎晋史, 下桐猛, 大島一郎, 片平清美, 印牧美佐生, 国枝哲夫, 万年英之

  Kuchinoshima cattle are unique feral cattle in Kuchinoshima Island in Kagoshima prefecture. We genotyped 54K SNPs for Kuchinoshima cattle, Japanese Black, Japanese Brown and Japanese Holstein using high density SNP chip to estimate genetic diversity and genetic structure in Kuchinoshima cattle. Average minor allele frequency in Kuchinoshima cattle was lower (0.089) than those of the other populations (0.181-0.251). Ratio of monomorphic SNPs for each chromosome in Kuchinoshima cattle ranged from 47.7% (BTA24) to 72.3% (BTA20). Subsequently, we investigated expected heterozygosity in each chromosome, and observed six monomorphic regions longer than 5Mb, especially, the longest region extended 12.80Mb. This study revealed the extremely low genetic diversity of Kuchinoshima cattle, due to founder effect derived from a few ancestral individuals and genetic drift caused by small population with below 100 animals lasting for a long time in Kuchinoshima Island.

  Japanese Society of Animal Science, Aug. 2016, 日本畜産学会報, 87(3) (3), 219 - 226, Japanese

  [Refereed]

  Scientific journal


• Allelic frequencies and association with carcass traits of six genes in local subpopulations of Japanese Black cattle

  Takahiro Nishimaki, Takayuki Ibi, Siqintuya, Naohiko Kobayashi, Tamako Matsuhashi, Takayuki Akiyama, Emi Yoshida, Kazumi Imai, Mayu Matsui, Keiichi Uemura, Hisayoshi Eto, Naoto Watanabe, Tatsuo Fujita, Yosuke Saito, Tomohiko Komatsu, Hiroshi Hoshiba, Hideyuki Mannen, Shinji Sasazaki, Tetsuo Kunieda

  Apr. 2016, ANIMAL SCIENCE JOURNAL, 87(4) (4), 469 - 476, English

  [Refereed]

  Scientific journal


• Genetic structure and relationships of 16 Asian and European cattle populations using DigiTag2 assay

  Riku Yonesaka, Shinji Sasazaki, Hiroshi Yasue, Satoru Niwata, Yousuke Inayoshi, Fumio Mukai, Hideyuki Mannen

  Feb. 2016, ANIMAL SCIENCE JOURNAL, 87(2) (2), 190 - 196, English

  [Refereed]

  Scientific journal


• The effect of leptin gene polymorphisms on fertility traits in Japanese Black cattle

  Fuki KAWAGUCHI, Kenji FUKAZAWA, Namiko KOHAMA, Takayuki AKIYAMA, Moriyuki FUKUSHIMA, Eiji KOBAYASHI, Kenji OYAMA, Hideyuki MANNEN, Shinji SASAZAKI

  Corresponding, Japanese Society of Animal Science, 2016, Nihon Chikusan Gakkaiho, 87(4) (4), 333 - 338, Japanese

  [Refereed]

  Scientific journal


• The g.841G > C SNP of FASN gene is associated with fatty acid composition in beef cattle

  Kiri Hayakawa, Takayuki Sakamoto, Atsushi Ishii, Keita Yamaji, Yoshinobu Uemoto, Nanae Sasago, Eiji Kobayashi, Naohiko Kobayashi, Tamako Matsuhashi, Shin Maruyama, Hirokazu Matsumoto, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Corresponding, Aug. 2015, ANIMAL SCIENCE JOURNAL, 86(8) (8), 737 - 746, English

  [Refereed]

  Scientific journal


• Paternal phylogeography and genetic diversity of East Asian goats

  A. Waki, S. Sasazaki, E. Kobayashi, H. Mannen

  Jun. 2015, ANIMAL GENETICS, 46(3) (3), 337 - 339, English

  [Refereed]

  Scientific journal


• UTS2R gene polymorphisms are associated with fatty acid composition in Japanese beef cattle

  Shinji Sasazaki, Kento Akiyama, Takahiro Narukami, Hirokazu Matsumoto, Kenji Oyama, Hideyuki Mannen

  Lead, May 2014, ANIMAL SCIENCE JOURNAL, 85(5) (5), 499 - 505, English

  [Refereed]

  Scientific journal


• The SNPs in the promoter regions of the bovine FADS2 and FABP4 genes are associated with beef quality traits

  Hirokazu Matsumoto, Takuya Nogi, Ichiro Tabuchi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  May 2014, LIVESTOCK SCIENCE, 163, 34 - 40, English

  [Refereed]

  Scientific journal


• Numerical analysis of intensity signals resulting from genotyping pooled DNA samples in beef cattle and broiler chicken

  A. Reverter, J. M. Henshall, R. McCulloch, S. Sasazaki, R. Hawken, S. A. Lehnert

  May 2014, JOURNAL OF ANIMAL SCIENCE, 92(5) (5), 1874 - 1885, English

  [Refereed]

  Scientific journal


• Evidence for pleiotropism and recent selection in the PLAG1 region in Australian Beef cattle

  M. R. S. Fortes, K. Kemper, S. Sasazaki, A. Reverter, J. E. Pryce, W. Barendse, R. Bunch, R. McCulloch, B. Harrison, S. Bolormaa, Y. D. Zhang, R. J. Hawken, M. E. Goddard, S. A. Lehnert

  Dec. 2013, ANIMAL GENETICS, 44(6) (6), 636 - 647, English

  [Refereed]

  Scientific journal


• Effects of genes on economically important traits of Japanese Black cattle in Hyogo population

  Ohkura K, Akiyama T, YOSHIDA E, FUKUSHIMA M, IWAMOTO E, OKA A, MATSUMOTO H, SASAZAKI Shinji, OYAMA Kenji, MANNEN Hideyuki

  In this study, we investigated allelic frequencies and genetic effects of SCD, SREBP-1, FASN, EDG-1, F11, MC1R and CW-2 genes on beef carcass traits including fatty acid composition in the closed Hyogo population of Japanese Black cattle. The favorable allele frequencies were 0.960 in SCD, 0.322 in SREBP-1, 0.910 in FASN, 0.265 in EDG-1, 0.623 in F11, 0.464 in MC1R and 0.007 in

  Nihon Chikusan Gakkai, Nov. 2013, Nihon Chikusan Gakkaiho, 84(2) (2), 157 - 162, Japanese

  [Refereed]

  Scientific journal


• Mitochondrial genetic diversity of goat in South Eastern Asia

  Kato T, Takahashi A, Matsumoto H, SASAZAKI Shinji, FARUQUE O, MASANGKAY JS, Nomura K, Takahashi Y, Amano T, Yamamoto Y, Namikawa T, MANNEN Hideyuki

  In this study, we determined 481bp hypervariable sequence of mitochondrial DNA D-loop region for Bangladeshi and Philippine native goats. The mitochondrial genetic diversity was estimated using obtained sequences, and then the origins of predominance mitochondrial lineage A and B in South East Asia were investigated. The analyses of 53 Bangladesh and 30 Philippine goats reveale

  Nihon Chikusan Gakkai, Nov. 2013, Nihon Chikusan Gakkaiho, 84(2) (2), 149 - 155, Japanese

  [Refereed]

  Scientific journal


• Genome-wide association study for fatty acid composition in Japanese Black cattle

  Atsushi Ishii, Keita Yamaji, Yoshinobu Uemoto, Nanae Sasago, Eiji Kobayashi, Naohiko Kobayashi, Tamako Matsuhashi, Shin Maruyama, Hirokazu Matsumoto, Shinji Sasazaki, Hideyuki Mannen

  Oct. 2013, ANIMAL SCIENCE JOURNAL, 84(10) (10), 675 - 682, English

  [Refereed]

  Scientific journal


• The assessment of genetic diversity within and among the eight subpopulations of Japanese Black cattle using 52 microsatellite markers

  Takahiro Nishimaki, Takayuki Ibi, Yoshihiro Tanabe, Yoshiyuki Miyazaki, Naohiko Kobayashi, Tamako Matsuhashi, Takayuki Akiyama, Emi Yoshida, Kazumi Imai, Mayu Matsui, Keiichi Uemura, Naoto Watanabe, Tatsuo Fujita, Yosuke Saito, Tomohiko Komatsu, Takahisa Yamada, Hideyuki Mannen, Shinji Sasazaki, Tetsuo Kunieda

  Aug. 2013, ANIMAL SCIENCE JOURNAL, 84(8) (8), 585 - 591, English

  [Refereed]

  Scientific journal


• Identification of quantitative trait loci affecting economic traits based on divergently selected genomic regions between beef and dairy cattle

  Keita Yamaji, Daisuke Hosokawa, Atsushi Ishii, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  Elsevier, Aug. 2013, Livestock Science, 155(2-3) (2-3), 180 - 185, English

  [Refereed]

  Scientific journal


• Genetic diversity and structure in Asian native goat analyzed by newly developed SNP markers

  Bang Zhong Lin, Taiki Kato, Makoto Kaneda, Hirokazu Matsumoto, Shinji Sasazaki, Hideyuki Mannen

  Aug. 2013, ANIMAL SCIENCE JOURNAL, 84(8) (8), 579 - 584, English

  [Refereed]

  Scientific journal


• Comparative analysis on gene expression profiles in longissimus dorsi muscle of Japanese Black cattle

  MATSUMOTO H, OGURI Y, MIZUNO Y, ISHIDA T, KOBAYASHI I, IWAISAKI H, SAITO K, SASAGO N, KOBAYASHI E, KUGE S, HONDA Takeshi, SASAZAKI Shinji, OYAMA Kenji, MANNEN Hideyuki

  Recently, fatty acid composition has been recognized as an important economic trait of Japanese Black cattle. The aim of this study was to seek for candidate genes which might be useful for beef breeding to increase the mono-unsaturated fatty acid (MUFA) level. For this purpose, we compared gene expression profile of Japanese Black cattle which showed high percentage of MUFA in

  日本動物遺伝育種学会, Jun. 2013, The Journal of Animal Genetics, 41(1) (1), 7 - 14, Japanese

  [Refereed]

  Scientific journal


• The SNP in the promoter region of the bovine ELOVL5 gene influences economic traits including subcutaneous fat thickness

  Hirokazu Matsumoto, Yumiko Shimizu, Atsuko Tanaka, Takuya Nogi, Ichiro Tabuchi, Kenji Oyama, Masaaki Taniguchi, Hideyuki Mannen, Shinji Sasazaki

  Apr. 2013, MOLECULAR BIOLOGY REPORTS, 40(4) (4), 3231 - 3237, English

  [Refereed]

  Scientific journal


• Molecular phylogeography and genetic diversity of East Asian goats

  B. Z. Lin, S. Odahara, M. Ishida, T. Kato, S. Sasazaki, K. Nozawa, H. Mannen

  Feb. 2013, ANIMAL GENETICS, 44(1) (1), 79 - 85, English

  [Refereed]

  Scientific journal


• The SNPs in the ACACA gene are effective on fatty acid composition in holstein milk

  Hirokazu Matsumoto, Kenta Sasaki, Takuya Bessho, Eiji Kobayashi, Tsuyoshi Abe, Shinji Sasazaki, Kenji Oyama, Hideyuki Mannen

  Sep. 2012, MOLECULAR BIOLOGY REPORTS, 39(9) (9), 8637 - 8644, English

  [Refereed]

  Scientific journal


• Sampling method for estimating neutral allele frequency in a pedigreed population

  Takeshi Honda, Shinji Sasazaki, Kenji Oyama, Fumio Mukai, Tetsuro Nomura

  Jun. 2012, JOURNAL OF ANIMAL BREEDING AND GENETICS, 129(3) (3), 226 - 233, English

  [Refereed]

  Scientific journal


• Identification of SNPs in the FASN gene and their effect on fatty acid milk composition in Holstein cattle

  Hirokazu Matsumoto, Satoshi Inada, Eiji Kobayashi, Tsuyoshi Abe, Hiroyuki Hasebe, Shinji Sasazaki, Kenji Oyama, Hideyuki Mannen

  Apr. 2012, LIVESTOCK SCIENCE, 144(3) (3), 281 - 284, English

  [Refereed]

  Scientific journal


• Identification of divergently selected regions between Japanese Black and Holstein cattle using bovine 50k SNP array

  Daisuke Hosokawa, Atsushi Ishii, Keita Yamaji, Shinji Sasazaki, Kenji Oyama, Hideyuki Mannen

  2012, ANIMAL SCIENCE JOURNAL, 83(1) (1), 7 - 13, English

  [Refereed]

  Scientific journal


• 黒毛和種におけるDGAT1遺伝子多型の枝肉形質に対する効果

  鳴神貴太, MANNEN HIDEYUKI, OYAMA KENJI, 庄司則章, 中嶋宏明, SASAZAKI SHINJI

  日本畜産学会, Nov. 2011, 日本畜産学会報, 82(2) (2), 125 - 130, Japanese

  [Refereed]

  Scientific journal


• Development of discrimination markers between Japanese domestic and imported beef

  Shinji Sasazaki, Daisuke Hosokawa, Rie Ishihara, Hiroki Aihara, Kenji Oyama, Hideyuki Mannen

  2011, ANIMAL SCIENCE JOURNAL, 82(1) (1), 67 - 72, English

  [Refereed]

  Scientific journal


• Effect of DNA polymorphisms related to fatty acid composition in adipose tissue of Holstein cattle

  Takahiro Narukami, Shinji Sasazaki, Kenji Oyama, Takuya Nogi, Masaaki Taniguchi, Hideyuki Mannen

  2011, ANIMAL SCIENCE JOURNAL, 82(3) (3), 406 - 411, English

  [Refereed]

  Scientific journal


• Allele frequencies of gene polymorphisms related to economic traits in Bos taurus and Bos indicus cattle breeds

  Makoto Kaneda, Bang Zhong Lin, Shinji Sasazaki, Kenji Oyama, Hideyuki Mannen

  2011, ANIMAL SCIENCE JOURNAL, 82(6) (6), 717 - 721, English

  [Refereed]

  Scientific journal


• Suppression of WWP1 Gene Via RNAi Induced the Reduction of Proliferation Rate of C2C12 Myoblasts

  Hirokazu Matsumoto, Michihiro Takahama, Ryohsuke Kajiyama, Shinji Sasazaki, Kenji Oyama, Hideyuki Mannen

  Oct. 2010, JOURNAL OF POULTRY SCIENCE, 47(4) (4), 288 - 293, English

  [Refereed]

  Scientific journal


• Accumulation of caveolin-3 protein is limited in damaged muscle in chicken muscular dystrophy

  Hirokazu Matsumoto, Shinji Sasazaki, Akira Fujiwara, Nobutsune Ichihara, Tateki Kikuchi, Hideyuki Mannen

  Sep. 2010, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 157(1) (1), 68 - 72, English

  [Refereed]

  Scientific journal


• Mitochondrial DNA variation and genetic construction of indigenous cattle population in Bhutan

  Dorji T, Kaneda M, Lin BZ, Takahashi A, OYAMA Kenji, SASAZAKI Shinji, Yamamoto Y, Kawamoto Y, MANNEN Hideyuki

  Japanese Society of Animal Genetics, Jun. 2010, Journal of Animal Genetics, 38(1) (1), 77 - 81, English

  [Refereed]

  Scientific journal


• Development of breed identification markers based on a bovine 50K SNP array

  Y. Suekawa, H. Aihara, M. Araki, D. Hosokawa, H. Mannen, S. Sasazaki

  Jun. 2010, MEAT SCIENCE, 85(2) (2), 285 - 288, English

  [Refereed]

  Scientific journal


• Mutated WWP1 Induces an Aberrant Expression of Myosin Heavy Chain Gene in C2C12 Skeletal Muscle Cells

  Hirokazu Matsumoto, Yumi Inba, Shinji Sasazaki, Akira Fujiwara, Nobutsune Ichihara, Tateki Kikuchi, Hideyuki Mannen

  Apr. 2010, JOURNAL OF POULTRY SCIENCE, 47(2) (2), 115 - 119, English

  [Refereed]

  Scientific journal


• Zebu Cattle Are an Exclusive Legacy of the South Asia Neolithic

  Shanyuan Chen, Bang-Zhong Lin, Mumtaz Baig, Bikash Mitra, Ricardo J. Lopes, Antonio M. Santos, David A. Magee, Marisa Azevedo, Pedro Tarroso, Shinji Sasazaki, Stephane Ostrowski, Osman Mahgoub, Tapas K. Chaudhuri, Ya-ping Zhang, Vania Costa, Luis J. Royo, Felix Goyache, Gordon Luikart, Nicole Boivin, Dorian Q. Fuller, Hideyuki Mannen, Daniel G. Bradley, Albano Beja-Pereira

  Jan. 2010, MOLECULAR BIOLOGY AND EVOLUTION, 27(1) (1), 1 - 6, English

  [Refereed]

  Scientific journal


• Sheep genetic diversity in Bhutan using microsatellite markers

  Tashi Dorji, Han Jianlin, Po Wafula, Yoshio Yamamoto, Shinji Sasazaki, Kenji Oyama, Oliver Hanotte, Bang-Zhong Lin, Hideyuki Mannen

  2010, ANIMAL SCIENCE JOURNAL, 81(2) (2), 145 - 151, English

  [Refereed]

  Scientific journal


• Development of SNP markers for individual identification and parentage test in a Japanese Black cattle population

  Kazuhiro Hara, Hideki Watabe, Shinji Sasazaki, Fumio Mukai, Hideyuki Mannen

  2010, ANIMAL SCIENCE JOURNAL, 81(2) (2), 152 - 157, English

  [Refereed]

  Scientific journal


• Genetic diversity and structure in Bos taurus and Bos indicus populations analyzed by SNP markers

  Bang Zhong Lin, Shinji Sasazaki, Hideyuki Mannen

  2010, ANIMAL SCIENCE JOURNAL, 81(3) (3), 281 - 289, English

  [Refereed]

  Scientific journal


• Development of novel SNP system for individual and pedigree control in a Japanese Black cattle population using whole-genome genotyping assay

  Kazuhiro Hara, Yukari Kon, Shinji Sasazaki, Fumio Mukai, Hideyuki Mannen

  2010, ANIMAL SCIENCE JOURNAL, 81(4) (4), 506 - 512, English

  [Refereed]

  Scientific journal


• Haplotype analysis revealed candidate region for black/brown coat color gene in cattle

  SASAZAKI Shinji, Usui M, Yoshozaki Y, Taniguchi M, Hasebe H, Abe T, Kobayashi E, MANNEN Hideyuki

  MC1R gene is known to be the main regulator of the switch between black and red coat color pigments in cattle. However, another gene would be associated with black and brown coat colors of cattle because our previous study reported that there were different coat color animals with the same MC1R genotype. The aim of this study was to identify the candidate region related to black/brown coat color in cattle. For this purpose, we genotyped 313 microsatellite markers evenly distributed across all cattle autosomes in the F₂ Japanese Black × Limousin resource family. In addition, 36 microsatellite markers located within candidate regions were developed in order to narrow down the candidate region. Our results revealed that the responsible gene for black/brown coat color would be included in the region from 16.1Mb to 26.5Mb on BTA 16.

  Japanese Society of Animal Genetics, Jun. 2009, Journal of Animal Genetics, 37(1) (1), 3 - 8, English

  [Refereed]

  Scientific journal


• brown coat color gene in cattle

  SASAZAKI Shinji, Usui M, Yoshozaki Y, Taniguchi M, Hasebe H, Abe T, Kobayashi E, MANNEN Hideyuki

  MC1R gene is known to be the main regulator of the switch between black and red coat color pigments in cattle. However, another gene would be associated with black and brown coat colors of cattle because our previous study reported that there were different coat color animals with the same MC1R genotype. The aim of this study was to identify the candidate region related to black/brown coat color in cattle. For this purpose, we genotyped 313 microsatellite markers evenly distributed across all cattle autosomes in the F₂ Japanese Black × Limousin resource family. In addition, 36 microsatellite markers located within candidate regions were developed in order to narrow down the candidate region. Our results revealed that the responsible gene for black/brown coat color would be included in the region from 16.1Mb to 26.5Mb on BTA 16.

  Japanese Society of Animal Genetics, Jun. 2009, Journal of Animal Genetics, 37(1) (1), 3 - 8, English

  [Refereed]

  Scientific journal


• Effect of SCD and SREBP genotypes on fatty acid composition in adipose tissue of Japanese Black cattle herds

  Hideki Ohsaki, Atsuko Tanaka, Shogo Hoashi, Shinji Sasazaki, Kenji Oyama, Masaaki Taniguchi, Fumio Mukai, Hideyuki Mannen

  Jun. 2009, ANIMAL SCIENCE JOURNAL, 80(3) (3), 225 - 232, English

  [Refereed]

  Scientific journal


• Expression Pattern of WWP1 in Muscular Dystrophic and Normal Chickens

  Hirokazu Matsumoto, Hideaki Maruse, Shinji Sasazaki, Akira Ftijiwara, Shin'ichi Takeda, Nobutsune Ichihara, Tateki Kikuchi, Fumio Mukai, Hideyuki Mannen

  Apr. 2009, JOURNAL OF POULTRY SCIENCE, 46(2) (2), 95 - 99, English

  [Refereed]

  Scientific journal


• Genetic diversity of growth hormone receptor gene in cattle

  Bang Zhong Lin, Shinji Sasazaki, June Heon Lee, Hideyuki Mannen

  2009, ANIMAL SCIENCE JOURNAL, 80(5) (5), 528 - 531, English

  [Refereed]

  Scientific journal


• Association between fatty acid compositions and genotypes of FABP4 and LXR-alpha in Japanese Black cattle

  Shogo Hoashi, Tomoko Hinenoya, Atsuko Tanaka, Hideki Ohsaki, Shinji Sasazaki, Masaaki Taniguchi, Kenji Oyama, Fumio Mukai, Hideyuki Mannen

  Dec. 2008, BMC GENETICS, 9, 84, English

  [Refereed]

  Scientific journal


• Genetic diversity of prion protein gene in Asian native goat

  Shinji Sasazaki, T. Nagato, H. Mannen

  Nov. 2008, SMALL RUMINANT RESEARCH, 80(1-3) (1-3), 101 - 103, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The ubiquitin ligase gene (WWP1) is responsible for the chicken muscular dystrophy

  Hirokazu Matsumoto, Hideaki Maruse, Yumi Inaba, Kanako Yoshizawa, Shinji Sasazaki, Akira Fujiwara, Masahide Nishibori, Akinori Nakamura, Shin'ichi Takeda, Nobutsune Ichihara, Tateki Kikuchi, Fumio Mukai, Hideyuki Mannen

  Jun. 2008, FEBS LETTERS, 582(15) (15), 2212 - 2218, English

  [Refereed]

  Scientific journal


• Discrimination of Korean cattle (Hanwoo) using DNA markers derived from SNPs in bovine mitochondrial and SRY genes

  D. Yoon, Y. S. Kwon, K. Y. Lee, W. Y. Jung, S. Sasazaki, H. Mannen, J. T. Jeon, J. H. Lee

  Jan. 2008, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 21(1) (1), 25 - 28, English

  [Refereed]

  Scientific journal


• A new tandem repeat in bovine fibrinogen A alpha gene

  Yumi Inaba, Kei Tsurifune, Shinji Sasazaki, Hideyuki Mannen

  2008, ANIMAL BIOTECHNOLOGY, 19(2) (2), 122 - 126, English

  [Refereed]

  Scientific journal


• Genotype of bovine sterol regulatory element binding protein-1 (SREBP-1) is associated with fatty acid composition in Japanese Black cattle

  Shogo Hoashi, Nobuhisa Ashida, Hideki Ohsaki, Takeshi Utsugi, Shinji Sasazaki, Masaaki Taniguchi, Kenji Oyama, Fumio Mukai, Hideyuki Mannen

  Dec. 2007, MAMMALIAN GENOME, 18(12) (12), 880 - 886, English

  [Refereed]

  Scientific journal


• Stearoyl-CoA desaturase mRNA expression during bovine adipocyte differentiation in primary culture derived from Japanese Black and Holstein cattle

  H. Ohsaki, T. Sawa, S. Sasazaki, K. Kano, M. Taniguchi, F. Mukai, H. Mannen

  Nov. 2007, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 148(3) (3), 629 - 634, English

  [Refereed]

  Scientific journal


• Pinpointing the candidate region for muscular dystrophy in chickens with an abnormal muscle gene

  Hirokazu Matsumoto, Hideaki Maruse, Kanako Yoshizawa, Shinji Sasazaki, Akira Fujiwara, Takeki Kikuchi, Nobutsune Ichihara, Fumio Mukai, Hideyuki Mannen

  Oct. 2007, ANIMAL SCIENCE JOURNAL, 78(5) (5), 476 - 483, English

  [Refereed]

  Scientific journal


• Development of DNA markers for discrimination between domestic and imported beef

  S. Sasazaki, H. Mutoh, K. Tsurifune, H. Mannen

  Oct. 2007, MEAT SCIENCE, 77(2) (2), 161 - 166, English

  [Refereed]

  Scientific journal


• Genetic diversity of Bhutanese Cattle analyzed by mitochondrial DNA variation

  Lin B, Odahara S, Sasazaki S, Yamamoto Y, Namikawa T, Tanaka K, Dorji T, Tshering G, MUKAI Fumio, MANNEN Hideyuki

  2007, Journal of Animal Genetics, 35(1):5-10, 5 - 10, English

  [Refereed]

  Scientific journal


• Proteomic comparison between Japanese Black and Holstein cattle by two-dimensional gel electrophoresis and identification of proteins

  H. Ohsaki, M. Okada, S. Sasazaki, T. Hinenoya, T. Sawa, S. Iwanaga, H. Tsuruta, F. Mukai, H. Mannen

  Asian-Australasian Association of Animal Production Societies, 2007, Asian-Australasian Journal of Animal Sciences, 20(5) (5), 638 - 644, English

  [Refereed]

  Scientific journal


• Mitochondrial DNA variation and genetic relationships in Japanese and Korean cattle

  S. Sasazaki, S. Odahara, C. Hiura, F. Mukai, H. Mannen

  Oct. 2006, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 19(10) (10), 1394 - 1398, English

  [Refereed]

  Scientific journal


• Breed discrimination using DNA markers derived from AFLP in Japanese beef cattle

  S Sasazaki, T Imada, H Mutoh, K Yoshizawa, H Mannen

  Aug. 2006, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 19(8) (8), 1106 - 1110, English

  [Refereed]

  Scientific journal


• A comparative map of macrochromosomes between chicken and Japanese quail based on orthologous genes

  S. Sasazaki, T. Hinenoya, B. Lin, A. Fujiwara, H. Mannen

  Aug. 2006, ANIMAL GENETICS, 37(4) (4), 316 - 320, English

  [Refereed]

  Scientific journal


• Mitochondrial DNA diversity of Korean native goats

  S Odahara, HJ Chung, SH Choi, SL Yu, S Sasazaki, H Mannen, CS Park, JH Lee

  Apr. 2006, ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 19(4) (4), 482 - 485, English

  [Refereed]

  Scientific journal


• Mapping of expressed sequence tag markers with a cDNA-amplified fragment length polymorphism method in Japanese quail (Coturnix japonica)

  S Sasazaki, T Hinenoya, D Fujima, S Kikuchi, A Fujiwara, H Mannen

  Feb. 2006, ANIMAL SCIENCE JOURNAL, 77(1) (1), 42 - 46, English

  [Refereed]

  Scientific journal


• Development of breed discrimination markers in domestic beef

  SASAZAKI Shinji, MANNEN Hideyuki

  Japanese Society of Animal Breeding and Genetics, 2006, The journal of animal genetics, 34(1) (1), 41 - 45


• Construction of a genetic linkage map of Japanese quail (Coturnix japonica) based on AFLP and microsatellite markers

  S. Kikuchi, D. Fujima, S. Sasazaki, S. Tsuji, M. Mizutani, A. Fujiwara, H. Mannen

  Jun. 2005, Animal Genetics, 36(3) (3), 227 - 231, English

  Scientific journal


• Allele frequencies of the extension locus encoding the melanocortin-1 receptor in Japanese and Korean cattle

  Shinji Sasazaki, Munehiro Usui, Hideyuki Mannen, Chihiro Hiura, Soichi Tsuji

  Apr. 2005, Animal Science Journal, 76(2) (2), 129 - 132, English

  Scientific journal


• Development and Mapping of Microsatellite Markers derived from cDNA in Japanese Quail (Coturnix japonica)

  MANNEN Hideyuki, MURATA K, KIKUCHI S, FUJIMA D, SASAZAKI S, FUJIWARA A, TSUJI S

  Japan Poultry Science Association, 2005, J. Poult. Sci., 42:263-271(3) (3), 263 - 271, English

  Scientific journal


• Development of breed identification markers derived from AFLP in beef cattle

  S. Sasazaki, K. Itoh, S. Arimitsu, T. Imada, A. Takasuga, H. Nagaishi, S. Takano, H. Mannen, S. Tsuji

  Jun. 2004, Meat Science, 67(2) (2), 275 - 280, English

  Scientific journal


• An association study using AFLP markers and application to a beef cattle breeding population

  S. Tsuji, K. Itoh, S. Sasazaki, H. Mannen, K. Oyama, M. Shojo, F. Mukai

  Feb. 2004, Animal Genetics, 35(1) (1), 40 - 43, English

  Scientific journal


• Selective genotyping for marker assisted selection in Japanese Black cattle.

  SASAZAKI S, ITOH K, URATSUJI H, MANNEN Hideyuki, OYAMA Kenji, SHOJO Masayuki, MUKAI Fumio, TSUJI S

  2004, Proc. 29th Int. Conf. on Anim. Genet., p.138, English

  International conference proceedings


• Genealogical relationship between pedigree and microsatellite information and analysis of genetic structure of a highly inbred Japanese black cattle strain

  S. Sasazaki, T. Honda, M. Fukushima, K. Oyama, H. Mannen, F. Mukai, S. Tsuji

  Asian-Australasian Association of Animal Production Societies, 2004, Asian-Australasian Journal of Animal Sciences, 17(10) (10), 1355 - 1359, English

  Scientific journal


• Phylogenic analysis of five beef cattle breeds using AFLP marker.

  Sasazaki, S, Lee, E.J, Mannen, H, Kunieda, T, Sakurai, T, Yamauchi, K, Yeo, J.S, Tsuji, S

  2001, Anim. Sci. J., 72, 172

  [Refereed]

• 兵庫県黒毛和種集団における枝肉の画像解析形質を対象としたゲノムワイド関連解析

  笹崎 晋史

  May 2019, 畜産技術, 768, 16 - 20

  [Invited]


• 複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討

  山本雷斗, 川口芙岐, 秋山敬考, 吉田恵実, 小浜菜美子, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

  2019, 日本畜産学会大会講演要旨, 125th


• mtDNA塩基配列を用いたカザフスタン在来ウシの系統遺伝学的解析

  村田佳子, 山本義雄, 西堀正英, MEIRAT Bakthin, POLAT Kazymbet, MELDELOKOV Alyan, MARATBEK Suleimenov, 笹崎晋史, 万年英之

  2018, 日本畜産学会大会講演要旨, 124th


• 兵庫県黒毛和種集団におけるSLC27A6遺伝子多型のロース脂肪割合に対する効果の検討

  中島彩華, 栖原功太郎, 小林栄治, 福島護之, 吉田恵実, 秋山敬孝, 小浜菜美子, 大山憲二, 本多健, 万年英之, 笹崎晋史

  2018, 日本畜産学会大会講演要旨, 124th


• ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索

  川口芙岐, 木越洸利, 小浜菜美子, 秋山敬孝, 福島護之, 吉田恵実, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

  2018, 日本畜産学会大会講演要旨, 124th


• mtDNA配列を用いたカザフスタン5地域の在来ヤギにおける遺伝的構造の比較

  田端里彩, 高橋幸水, 野村こう, 山縣高宏, 山本義雄, 山本義雄, 角田健司, 角田健司, 国枝哲夫, 伊藤文香, 竹内佳子, BAKHTIN Meirat, KAZYMBET Polat, MELDELOKOV Alyan, SULEIMENOV Maratbek, 西堀正英, 笹崎晋史, 万年英之

  2017, 日本畜産学会大会講演要旨, 122nd


• Identification of SNP associated with fertility trait using pool-based genome-wide association study in Japanese Black cattle.

  H. Ozaki, T. Tamura, K. Fukazawa, Y. Uemoto, M. Nishio, E. Kobayashi, T. Matsuhashi, S. Maruyama, T. Honda, K. Oyama, S. Sasazaki, H. Mannen

  Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 21 - 21, English

  Summary international conference


• Identification of polymorphisms associated with oleic acid percentage by pool-based genome-wide association study in Japanese Black cattle.

  F. Kawaguchi, A. Nakajima, Y. Matsumoto, Y. Uemoto, M. Fukushima, E. Yoshida, E. Iwamoto, T. Akiyama, N. Kohama, E. Kobayashi, T. Honda, K. Oyama, H. Mannen, S. Sasazaki

  Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 17 - 18, English

  Summary international conference


• Genetic diversity and origin of mtDNA haplogroup P observed in Japanese Shorthorn.

  A. Noda, S. Sasazaki, H. Mannen

  Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 18 - 18, English

  Summary international conference


• Genome-wide association study identifies a QTL for fat percentage in ribeye area on BTA10 in Japanese Black cattle.

  A. Inoue, T. Nakajima, A. Nakajima, Y. Uemoto, M. Fukushima, E. Yoshida, E. Iwamoto, T. Akiyama, N. Kohama, E. Kobayashi, K. Oyama, T. Honda, H. Mannen, S. Sasazaki

  Sep. 2016, JOURNAL OF ANIMAL SCIENCE, 94, 17 - 17, English

  Summary international conference


• ニワトリ筋ジストロフィーに関する研究

  松本大和, 笹崎晋史, 万年英之

  岡山実験動物研究会, Apr. 2014, 岡山実験動物研究会報, (30) (30), 68 - 69, Japanese


• ウシFABP4遺伝子のプロモーター領域に対する多型探索

  松本大和, 大山憲二, 万年英之, 笹崎晋史

  日本動物遺伝育種学会, 01 Dec. 2012, 動物遺伝育種研究, 40(2) (2), 78 - 78, Japanese


• 岐阜県黒毛和種集団におけるUTS2R遺伝子内多型の脂肪酸組成に対する効果

  石井淳, 山地啓太, 阪本嵩幸, 秋山賢斗, 上本吉伸, 笹子奈々恵, 小林栄治, 小林直彦, 松橋珠子, 丸山新, 松本大和, 笹崎晋史, 大山憲二, 万年英之

  01 Dec. 2012, 動物遺伝育種研究, 40(2) (2), 73, Japanese


• 黒毛和種におけるLEP遺伝子内多型の脂肪酸組成に対する効果

  大倉一輝, 松本大和, 笹崎晋史, 大山憲二, 万年英之

  01 Dec. 2012, 動物遺伝育種研究, 40(2) (2), 85, Japanese


• 黒毛和種におけるFASN遺伝子内多型の脂肪酸組成に対する効果

  阪本嵩幸, 石井淳, 山地啓太, 早川きり, 上本吉伸, 笹子奈々恵, 高須賀晶子, 小林栄治, 小林直彦, 松橋珠子, 丸山新, 平野貴, 杉本喜憲, 松本大和, 笹崎晋史, 大山憲二, 万年英之

  01 Dec. 2012, 動物遺伝育種研究, 40(2) (2), 72, Japanese


• 黒毛和種の枝肉形質に対するSCD,SREBP1,FASNおよびNCAPG遺伝子多型の関連解析

  西牧孝洋, 揖斐隆之, 斯琴図雅, 小林直彦, 松橋珠子, 今井佳積, 松井茉由, 上村圭一, 衛藤央好, 渡邉直人, 藤田達男, 齊藤陽介, 小松智彦, 万年英之, 笹崎晋史, 国枝哲夫

  01 Dec. 2012, 動物遺伝育種研究, 40(2) (2), 71, Japanese


• プーリング法を用いた高密度SNP解析による黒毛和種閉鎖集団の遺伝的多様性の評価

  加藤 大樹, 松本 大和, 笹崎 晋史, 秋山 敬孝, 吉田 恵実, 福島 護之, 野村 哲郎, 万年 英之

  (公社)日本畜産学会, Mar. 2012, 日本畜産学会大会講演要旨集, 115回, 118 - 118, Japanese


• 兵庫県黒毛和種肥育集団における経済形質関連遺伝子の遺伝構造

  大倉一輝, 松本大和, 秋山敬孝, 吉田恵実, 福島護之, 笹崎晋史, 万年英之

  01 Dec. 2011, 動物遺伝育種研究, 39(2) (2), 142, Japanese


• ホルスタイン集団におけるsliding window approachを利用した乳形質関連遺伝子座の探索

  山地啓太, 石井淳, 細川大介, 松本大和, 笹崎晋史, 大山憲二, 万年英之

  01 Dec. 2011, 動物遺伝育種研究, 39(2) (2), 133, Japanese


• 黒毛和種集団を用いた脂肪酸組成に関するゲノムワイド相関解析

  石井淳, 山地啓太, 阪本嵩幸, 上本吉伸, 笹子奈々恵, 小林栄治, 小林直彦, 松橋珠子, 丸山新, 松本大和, 笹崎晋史, 万年英之

  01 Dec. 2011, 動物遺伝育種研究, 39(2) (2), 135, Japanese


• 黒毛和種における各地域集団の遺伝的多様性と類縁関係の解析

  西牧孝洋, 揖斐隆之, 田邉義弘, 宮崎義之, 小林直彦, 松橋珠子, 秋山敬孝, 吉田恵実, 今井佳積, 松井茉由, 上村圭一, 渡邉直人, 藤田達男, 齊藤陽介, 万年英之, 笹崎晋史, 国枝哲夫

  01 Dec. 2011, 動物遺伝育種研究, 39(2) (2), 142, Japanese


• Identification of the gene responsible for chicken muscular dystrophy

  Matsumoto H, SASAZAKI SHINJI, MANNEN HIDEYUKI

  The Korean Society of Poultry Science, Jun. 2011, Korean Journal of Poultry Science, 38(2) (2), 145 - 154, English

  [Refereed][Invited]

  Introduction scientific journal


• ニワトリ筋ジストロフィー責任遺伝子の同定とモデル動物としての展開

  MANNEN HIDEYUKI, SASAZAKI SHINJI, 松本大和

  関西実験動物研究会, Dec. 2010, 関西実験動物研究会会報, 32, 1 - 12, Japanese

  [Invited]

  Introduction scientific journal


• ニワトリ筋ジストロフィー原因遺伝子の同定

  松本大和, SASAZAKI SHINJI, MANNEN HIDEYUKI

  日本動物遺伝育種学会, Jun. 2010, 動物遺伝育種研究, 38(1) (1), 21 - 28, Japanese

  [Refereed]

  Introduction scientific journal


• ニワトリ筋ジストロフィーにおけるcaveolin-3の発現解析

  松本 大和, 笹崎 晋史, 藤原 哲, 菊池 建機, 市原 伸恒, 万年 英之

  (公社)日本畜産学会, Sep. 2009, 日本畜産学会大会講演要旨集, 111回, 41 - 41, Japanese

• アグリバイオー和牛の育種改良

  笹崎 晋史

  北隆館, Jan. 2020


• 兵庫の和牛 但馬牛

  SASAZAKI SHINJI

  Others, 兵庫県農政環境部農林水産局畜産課, Mar. 2018, Japanese

  Scholarly book


• 食のバイオ計測の最前線―機能解析と安全・安心の計測を目指して―「DNA鑑定を利用した牛肉偽装表示の防止」

  MANNEN HIDEYUKI, SASAZAKI SHINJI

  Others, ㈱シーエムシー出版, May 2011, Japanese

  Scholarly book


• 食品表示を裏づける分析技術 科学の目で偽装を見破る 「品種鑑別 牛」

  MANNEN HIDEYUKI, SASAZAKI SHINJI

  Joint editor, 日本分析化学 表示･期限分析技術研究懇談会編, Nov. 2010, Japanese

  Scholarly book

• ウシ6番染色体の脂肪酸組成QTLに対する責任多型の探索

  伊藤亮太朗, 吉田恵美, 岩本英治, 大山憲二, 万年英之, 笹崎晋史, 川口芙岐

  第25回日本動物遺伝育種学会大会, Nov. 2024


• ミトゲノム解析による東南アジア在来ヤギの伝播過程の推定

  小楠夏帆, 笹崎晋史, 川口芙岐, 田中啓介, 呉佳齊, 米澤隆弘, 万年英之

  第25回日本動物遺伝育種学会大会, Nov. 2024


• 岩手県黒毛和種における白斑発生に関わるMITF遺伝子多型の探索

  小出毅大, 西和隆, 川口芙岐, 万年英之, 笹崎晋史

  第132回日本畜産学会大会, Sep. 2024


• 兵庫県黒毛和種の19番染色体における脂肪酸組成に対する責任多型の探索

  橋本晃太郎, 小浜菜美子, 吉田恵実, 岩本英治, 大山憲二, 万年英之, 笹崎晋史, 川口芙岐

  第132回日本畜産学会大会, Sep. 2024


• Exploring the gene polymorphisms associated with high-altitude adaptation in Nepalese goat breeds using Whole-genome resequencing

  Ryo Masuko, Natsumi Murai, Fuki Kawaguchi, Hideyuki Mannen, Shinji Sasazaki

  31th Plant & Animal Genome Conference, Jan. 2024


• The previously reported 11.7 kb deletion on caprine chromosome 1 is responsible for polledness in Asian goats

  Fuki Kawaguchi, Ryo Morisawa, Shinji Sasazaki, Hideyuki Mannen

  31th Plant & Animal Genome Conference, Jan. 2024


• Comprehensive verification of all SNPs within a QTL region suggested that ICAM gene polymorphisms would be responsible for beef marbling in Japanese Black cattle

  Shinji Sasazaki, Hina Kondo, Yurika Moriishi, Fuki Kawaguchi, Kenji Oyama, Hideyuki Mannen

  31th Plant & Animal Genome Conference, Jan. 2024


• ミトゲノム解析によるアジア在来ヤギハプログループBの起源および伝播経路の推定

  井上真梨, 笹崎晋史, 川口芙岐, 田中啓介, 米澤隆弘, 呉佳齊, 万年英之

  日本動物遺伝育種学会第24回大会, Nov. 2023


• 兵庫県黒毛和種集団を用いた17番染色体における脂肪交雑QTLの探索

  伊藤ひかり, 足立亮仁, 川口芙岐, 万年英之, 笹崎晋史

  日本動物遺伝育種学会第24回大会, Nov. 2023


• 全ゲノムリシーケンスデータを用いたヤギ高地適応遺伝子多型の探索

  村井菜摘, 増子諒, 川口芙岐, 万年英之, 笹崎晋史

  日本動物遺伝育種学会第24回大会, Nov. 2023


• アジア在来ヤギにおける毛色の多様性に関わるMC1R遺伝子多型の探索

  釋 天音, 川口 芙岐, 万年 英之, 笹崎 晋史

  日本畜産学会第131回大会, Sep. 2023


• 高密度SNPマーカーを用いた東南アジア諸島在来ヤギの遺伝構造と伝播、遺伝子流入の推定

  増子諒, 高尾祐樹, アインアイン, 笹崎晋史, 川口芙岐, 山本義雄, Masangakay JS, Dagong MIA, Bugiwati SRA, Jiaqi Wu, 米澤隆弘, 万年英之

  日本畜産学会第131回大会, Sep. 2023


• 宮崎県黒毛和種集団を用いたウシ7番染色体における脂肪交雑に対する原因多型の探索

  森石 宥璃華, 川口 芙岐, 万年 英之, 笹崎 晋史

  日本畜産学会第131回大会, Sep. 2023


• Genome-Wide Association Study for the Percentage of Oleic Acid in Japanese Black Cattle Revealed the Candidate Region on BTA19

  Fuki Kawaguchi, Natsuki Maeda, Kenji Oyama, Emi Yoshida, Eiji Iwamoto, Namiko Kohama, Shinji Sasazaki, Hideyuki Mannen

  30th Plant & Animal Genome Conference, Jan. 2023, English


• Verification of Candidate SNP Effects Reveals Two QTLs on BTA7 for Beef Marbling in Two Japanese Black Cattle Populations

  Shinji Sasazaki, Raito Yamamoto, Shintaro Toyomoto, Hina Kondo, Namiko Kohama, Emi Yoshida, Eiji Iwamoto, Fuki Kawaguchi, Kenji Oyama, Hideyuki Mannen

  30th Plant & Animal Genome Conference, Jan. 2023, English


• 岩手県黒毛和種における白斑とMITF遺伝子多型との関連

  金丸智香, 西和隆, 川口芙岐, 万年英之, 笹崎晋史

  日本動物遺伝育種学会第23回大会, Nov. 2022


• 兵庫県黒毛和種におけるゲノムワイド関連解析を用いたオレイン酸含有率QTLの探索

  前田菜月, 小浜菜美子, 秋山敬孝, 吉田恵実, 岩本英治, 大山憲二, 笹崎晋史, 万年英之, 川口芙岐

  日本畜産学会第130回大会, Sep. 2022


• アジア在来ヤギにおける角の有無と既報の1番染色体無角関連多型との関連

  森澤亮, 笹崎晋史, 万年英之, 川口芙岐

  日本動物遺伝育種学会第22回大会, Nov. 2021


• 父母系および核ゲノムSNPマーカーを用いたインドネシア在来ヤギの遺伝構造解析及び遺伝子流入の推定

  政岡真帆, 笹崎晋史, 川口芙岐, 米澤隆弘, 呉 佳齊, 山本義雄, DAGONG MIA, BUGIWATI SRA, 万年英之

  日本動物遺伝育種学会第22回大会, Nov. 2021


• 黒毛和種現場後代検定調査牛集団における全ゲノムリシーケンスデータを用いたオレイン酸含有率に関わる多型の探索

  緋田泰良, 笹崎晋史, 大山憲二, 万年英之, 川口芙岐

  日本畜産学会第129回大会, Sep. 2021


• Confirmation of quantitative trait locus location on BTA19 for the percentage of oleic acid in beef based on effects of 5 polymorphisms and linkage disequilibrium analysis in 2 Japanese Black cattle populations.

  Fuki Kawaguchi, Fuka Kakiuchi, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki

  38th International Society for Animal Genetics Conference, Jul. 2021


• 父母系および高密度 SNPマーカーを用いたフィリ ピン在来ヤギの遺伝構造と伝播の推定

  AYIN AYIN, 田端 里彩, 笹崎 晋史, 川口 芙岐, 小林 栄治, 山本 義雄, 米澤 隆弘, 呉 佳齊, Masangkay J, 万年 英之

  日本畜産学会第128回大会, Mar. 2021


• 50K SNPアレイを用いた和牛4品種に関する多様性および類縁関係の調査

  中村充暉, 鈴木啓一, 小林栄治, 万年英之, 笹崎晋史, 川口芙岐

  日本畜産学会第128回大会, Mar. 2021


• ネパール在来ヤギにおけるEPAS1, FGF5遺伝子多型と高地適応形質との関連調査

  冨田宏一郎, 野村悠登, 笹崎晋史, 万年英之

  第21回日本動物遺伝育種学会大会, Nov. 2020


• 集積流体回路を用いた兵庫県黒毛和種集団におけるロース脂肪割合候補多型の効果の検証

  近藤陽菜, 小浜菜美子, 秋山敬孝, 吉田恵実, 小林栄治, 川口芙岐, 万年英之, 笹崎晋史

  日本動物遺伝育種学会第21回大会, Nov. 2020


• ミトゲノム配列による ハプログループT4の系統解析

  角谷 菜帆, 村田 佳子, 川口 芙岐, 笹崎 晋史, 万年 英之

  第20回日本動物遺伝育種学会大会, Nov. 2019


• Validation of candidate SNPs for fat percentage in bovine rib-eye area using Integrated Fluidic Circuit

  Yamamoto R, Toyomoto S, Kohama N, Akiyama T, Yoshida E, Kobayashi E, Oyama K, Mannen H, Sasazaki S

  37th International Society for Animal Genetics Conference, Jul. 2019, English, Lleida, Spain, International conference

  Poster presentation


• Phylogenetic analysis of Nepalese goats using mtDNA D-loop region and SRY gene sequences

  Nomura Y, Kunieda T, Shah M, Kawaguchi F, Sasazaki S, Mannen H

  37th International Society for Animal Genetics Conference, Jul. 2019, English, Lleida, Spain, International conference

  Poster presentation


• Genetic diversity of Bos taurus and Bos indicus using developed indel markers

  Yasui T, Yamanaka H, Kawaguchi F, Sasazaki S, Yonezawa T, Wu J, Mannen H

  37th International Society for Animal Genetics Conference, Jul. 2019, English, Lleida, Spain, International conference

  Poster presentation


• Genetic diversity and structure in 11 native Asian goat populations analyzed by high density SNP array

  Iso K, Sasazaki S, Kawaguchi F, Yonezawa T, Wu J, Nomura K, Takahashi Y, Kobayashi E, Shah M, Faruque O, Masangkay JS, Bakhtin M, Kazymbet P, Dorji T, Mannen H

  37th International Society for Animal Genetics Conference, Jul. 2019, English, Lleida, Spain, International conference

  Poster presentation


• Effect of the PLAG1 gene polymorphism on carcass weight and oleic acid percentage in Japanese Black cattle populations

  Kawaguchi F, Kigoshi H, Yasuzumi R, Oyama K, Mannen H, Sasazaki S

  37th International Society for Animal Genetics Conference, Jul. 2019, English, Lleida, Spain, International conference

  Poster presentation


• Molecular epidemiological study of bovine leukemia virus in Zebu cattle (Bos indicus)

  Nishikaku K, Yonezawa T, Murakami S, Torii Y, Kawaguchi F, Sasazaki S, Mannen H, Kobayashi T

  The 2nd Joint Meeting of Veterinary Science in East Asia, Apr. 2019, English, Seoul, Korea, International conference

  Oral presentation


• 複数の黒毛和種集団を用いたSLC27A6遺伝子多型のBMSに対する効果の検討

  山本雷斗, 川口芙岐, 秋山 敬孝, 吉田 恵実, 小浜 菜美子, 小林 栄治, 大山 憲二, 万年 英之, 笹崎 晋史

  第125回日本畜産学会大会, Mar. 2019, Japanese, 日本畜産学会, 相模原, Domestic conference

  Oral presentation


• mtDNA D-loop領域とSRY遺伝子の塩基配列に基づいたネパール在来ヤギの遺伝的類縁関係

  野村悠登, 国枝哲夫, Manoj Shah, 笹崎晋史, 川口芙岐, 万年英之

  第125回日本畜産学会大会, Mar. 2019, Japanese, 日本畜産学会, 相模原, Domestic conference

  Oral presentation


• 兵庫県黒毛和種集団における全ゲノムリシーケンスデータを用いた枝肉形質に対する候補変異の抽出

  木越洸利, 川口芙岐, 大山憲二, 万年英之, 笹崎晋史

  第18回日本動物遺伝育種学会大会, Sep. 2018, Japanese, 京都, Domestic conference

  Oral presentation


• ゲノムリシーケンスデータを用いたウシ10番染色体におけるロース脂肪割合の原因変異の探索

  安栖梨理, 井上麻美, 小林 栄治, 福島 護之, 小浜 菜美子, 大山 憲二, 本多 健, 万年 英之, 笹崎 晋史

  第18回日本動物遺伝育種学会大会, Sep. 2018, Japanese, 京都, Domestic conference

  Oral presentation


• カザフスタン在来ヤギに対するmtDNAとY染色体の塩基配列解析～Eurasian Steppeは重要なヤギの伝播経路の一つである～

  田端里彩, 笹崎晋史, 山本義雄, Meirat BAKHTIN, Polat KAZYMBET, Meldelokov ALYAN, Maratbek Z SULEIMENOV, 西堀正英, 万年英之

  第18回日本動物遺伝育種学会大会, Sep. 2018, Japanese, 京都, Domestic conference

  Oral presentation


• Effect of SLC27A6 gene K81M polymorphism on fat percentage in rib-eye area in Japanese Black cattle

  Toyomoto S, Kohama N, Akiyama T, Fukushima M, Yoshida E, Kobayashi E, Oyama K, Mannen H, Sasazaki S

  PAG Asia 2018, May 2018, English, Scherago International, Seoul, Korea, International conference

  Poster presentation


• Detection of candidate polymorphisms in the QTL for oleic acid percentage on BTA9 based on whole-genome resequencing data

  Kawaguchi F, Kigoshi H, Kohama N, Akiyama T, Fukushima M, Yoshida E, Kobayashi E, Oyama K, Mannen H, Sasazaki S

  PAG Asia 2018, May 2018, English, Scherago International, Seoul, Korea, International conference

  Poster presentation


• 兵庫県黒毛和種集団におけるSLC27A6遺伝子多型のロース脂肪割合に対する効果の検討

  中島彩華, 栖原功太郎, 小林 栄治, 福島 護之, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 大山 憲二, 本多 健, 万年 英之, 笹崎 晋史

  第124回日本畜産学会大会, Mar. 2018, Japanese, 日本畜産学会, 東京, Domestic conference

  Oral presentation


• ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索

  川口芙岐, 木越洸利, 小浜菜美子, 秋山敬孝, 福島護之, 吉田恵実, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

  第124回日本畜産学会大会, Mar. 2018, Japanese, 日本畜産学会, 東京, Domestic conference

  Oral presentation


• mtDNA塩基配列を用いたカザフスタン在来ウシの系統遺伝学的解析

  村田佳子, 山本義雄, 西堀正英, Bakhtin M, Kazymbet P, Alyan M, Suleimenov MZ, 笹崎晋史, 万年英之

  第124回日本畜産学会大会, Mar. 2018, Japanese, 日本畜産学会, 東京, Domestic conference

  Oral presentation


• 兵庫県黒毛和種におけるゲノムワイド関連解析により同定されたVNN1遺伝子T66M多型のオレイン酸含有率に対する効果

  木越洸利, 川口芙岐, 上本吉伸, 福島護之, 吉田恵実, 岩本英治, 秋山敬孝, 小浜菜美子, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

  第18回日本動物遺伝育種学会大会, Nov. 2017, Japanese, 動物遺伝育種学会, 厚木, Domestic conference

  Oral presentation


• 複数の黒毛和種集団を用いたPLAG1遺伝子多型のオレイン酸含有率に対する効果の検討

  川口芙岐, 木越洸利, 安栖梨理, 小浜菜美子, 秋山敬孝, 福島護之, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

  第18回日本動物遺伝育種学会大会, Nov. 2017, Japanese, 動物遺伝育種学会, 厚木, Domestic conference

  Oral presentation


• 北海道ホルスタインは希少mtDNAハプログループPを有する

  野田葵, 笹崎晋史, 小林栄治, 万年英之

  第67回関西畜産学会大会, Sep. 2017, Japanese, 関西畜産学会, 大阪, Domestic conference

  Oral presentation


• ゲノムリシーケンスデータを利用したウシ7番染色体におけるロース脂肪割合に関わる遺伝子変異の探索

  中島彩華, 中嶋翼, 上本吉伸, 小林 栄治, 福島 護之, 岩本 英治, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 大山 憲二, 本多 健, 万年 英之, 笹崎 晋史

  第67回関西畜産学会大会, Sep. 2017, Japanese, 関西畜産学会, 大阪, Domestic conference

  Oral presentation


• Indelマーカーを用いた家畜ウシ11集団に対する遺伝的構造および系統解析

  山中颯, 笹崎晋史, Hla Hla Moe, Moe Lwin, 下桐猛, 万年英之

  第67回関西畜産学会大会, Sep. 2017, Japanese, 関西畜産学会, 大阪, Domestic conference

  Oral presentation


• PLAG1 polymorphism (ss319607405) is associated with oleic acid percentage in Japanese Black cattle

  Kawaguchi F, kigoshi H, Nakajima A, Matsumoto Y, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Honda T, Oyama K, Mannen H, Sasazaki S

  36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

  Poster presentation


• Phylogenetic analysis of Kazakhstani goats using mtDNA HV1 and SRY gene sequences

  Tabata R, Sasazaki S, Bakhtin M, Kazymbet P, Alyan M, Suleimenov M, Nishibori M, Mannen H

  36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

  Poster presentation


• Genome-wide association study revealed a candidate region for beef marbling on BTA7 in Japanese Black cattle

  Sasazaki S, Nakajima T, Nakajima A, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Oyama K, Mannen H

  36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

  Poster presentation


• Genetic structure and relationships among 11 cattle populations using Indel markers

  Yamanaka H, Sasazaki S, Moe HH, Lwin M, Shimogiri T, Mannen H

  36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

  Poster presentation


• Effects of DNA markers associated with carcass traits and fatty acid composition on fertility traits in Japanese Black cows

  Tsuchimura M, Fukazawa K, Matsuhashi T, Maruyama S, Oyama K, Sasazaki S, Mannen H

  36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

  Poster presentation


• Association of VNN1 gene polymorphism with fatty acid composition in Japanese Black cattle

  Kigoshi H, Matsumoto Y, Kawaguchi F, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Oyama K, Honda T, Mannen H, Sasazaki S

  36th International Society for Animal Genetics Conference, Jul. 2017, English, International Society for Animal Genetics, Dublin, Ireland, International conference

  Poster presentation


• mtDNA配列を用いたカザフスタン5地域の在来ヤギにおける遺伝的構造の比較

  田端里彩, 高橋幸水, 野村こう, 山縣高宏, 山本義雄, 角田健司, 国枝哲夫, 伊藤文香, 竹内佳子, Bakhtin M, Kazymbet P, 西堀正英, Alyan M, Suleimenov MZ, 笹崎晋史, 万年英之

  第122回日本畜産学会大会, Mar. 2017, Japanese, 日本畜産学会, 神戸, Domestic conference

  Oral presentation


• 黒毛和種繁殖雌牛集団におけるレプチン遺伝子内多型と繁殖形質との関連

  川口芙岐, 深澤謙治, 小浜菜美子, 秋山敬孝, 福島護之, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

  第17回日本動物遺伝育種学会大会, Nov. 2016, Japanese, 日本動物遺伝育種学会, 名古屋, Domestic conference

  Oral presentation


• Identification of SNP associated with fertility trait using pool-based genome-wide association study in Japanese Black cattle

  Ozaki H, Tamura T, Fukazawa K, Uemoto Y, Nishio M, Kobayashi E, Matsuhashi T, Maruyama S, Honda T, Oyama K, Sasazaki S, Mannen H

  35th International Society for Animal Genetics Conference, Jul. 2016, English, International Society for Animal Genetics, Slat Lake City, USA, International conference

  Poster presentation


• Identification of polymorphisms associated with oleic acid percentage by pool-based genome wide association study (GWAS) in Japanese Black

  Kawaguchi F, Nakajima A, Matsumoto Y, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Honda T, Oyama K, Mannen H, Sasazaki S

  35th International Society for Animal Genetics Conference, Jul. 2016, English, International Society for Animal Genetics, Slat Lake City, USA, International conference

  Poster presentation


• Genome-wide association study identifies a QTL for fat percentage in rib-eye area on BTA10 in Japanese Black cattle

  Inoue A, Nakajima T, Nakajima A, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Oyama K, Mannen H, Sasazaki S

  35th International Society for Animal Genetics Conference, Jul. 2016, English, International Society for Animal Genetics, Slat Lake City, USA, International conference

  Poster presentation


• Genetic diversity and origin of mtDNA haplogroup P observed in Japanese Shorthorn

  Noda A, Sasazaki S, Mannen H

  35th International Society for Animal Genetics Conference, Jul. 2016, English, International Society for Animal Genetics, Slat Lake City, USA, International conference

  Poster presentation


• 日本短角種において観察される希少なmtDNAハプログループPの母系起源

  野田葵, 万年英之, 笹崎晋史

  第121回日本畜産学会大会, Mar. 2016, Japanese, 日本畜産学会, 東京, Domestic conference

  Oral presentation


• ウシ10番染色体におけるロース脂肪割合に影響する遺伝子の探索

  井上麻美, 中島彩華, 中嶋翼, 上本吉伸, 小林 栄治, 福島 護之, 岩本 英治, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 大山 憲二, 本多 健, 万年英之, 笹崎晋史

  第121回日本畜産学会大会, Mar. 2016, Japanese, 日本畜産学会, 東京, Domestic conference

  Poster presentation


• 兵庫県黒毛和種集団におけるオレイン酸含有率に対するゲノムワイド関連解析

  松本祐汰, 中島彩華, 上本 吉伸, 小林栄治, 福島 護之, 岩本 英治, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 笹崎晋史, 本多健, 大山憲二, 万年英之

  第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference

  Poster presentation


• 黒毛和種における産肉形質関連マーカーの繁殖形質に対する効果の推定

  深澤謙治, 笹崎晋史, 大山憲二, 本多健, 小林栄治, 福島 護之, 秋山 敬孝, 小浜 菜美子, 万年英之

  第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference

  Poster presentation


• 岐阜県黒毛和種の繁殖形質を対象としたゲノムワイド関連解析

  田村朋子, 深澤謙治, 尾崎弘樹, 上本吉伸, 西尾元秀, 小林栄治, 松橋珠子, 丸山新, 本多健, 笹崎晋史, 大山憲二, 万年英之

  第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference

  Poster presentation


• GWASを用いた黒毛和種集団におけるロース脂肪割合に関わる遺伝子座の探索

  中嶋翼, 中島彩華, 上本吉伸, 小林 栄治, 福島 護之, 岩本 英治, 吉田 恵実, 秋山 敬孝, 小浜 菜美子, 大山憲二, 本多健, 万年英之, 笹崎晋史

  第16回日本動物遺伝育種学会大会, Nov. 2015, Japanese, 神戸, Domestic conference

  Poster presentation


• 黒毛和種集団におけるLeptin遺伝子翻訳領域内多型の脂肪酸組成に対する効果

  川口芙岐, 大倉一輝, 大山憲二, 万年英之, 笹崎晋史

  第118回日本畜産学会大会, Mar. 2015, Japanese, 日本畜産学会, 宇都宮, Domestic conference

  Oral presentation


• 高密度SNPアレイによる口之島牛の遺伝的多様性解析

  齊藤 祐也, 下桐 猛, 笹崎晋史, 万年英之

  第118回日本畜産学会大会, Mar. 2015, Japanese, 日本畜産学会, 宇都宮, Domestic conference

  Oral presentation


• ホルスタイン種におけるFASN-g.841 及びg.16024 多型の乳形質に対する効果

  早川きり, 阿部剛, 長谷部浩行, 小林栄治, 大山憲二, 万年英之, 笹崎晋史

  第15回日本動物遺伝育種学会大会, Nov. 2014, Japanese, 日本動物遺伝育種学会, 和光, Domestic conference

  Poster presentation


• SRY 遺伝子とmtDNA 塩基配列を用いたシバヤギの遺伝的多様性解析

  脇愛生, 笹崎晋史, 小林栄治, 万年英之

  第15回日本動物遺伝育種学会大会, Nov. 2014, Japanese, 日本動物遺伝育種学会, 和光, Domestic conference

  Poster presentation


• DigiTag2 assay を用いたアジア牛集団に対する遺伝的類縁関係と遺伝的構造の解析

  米坂陸, 笹崎晋史, 安江博, 庭田悟, 向井文雄, 万年英之

  第15回日本動物遺伝育種学会大会, Nov. 2014, Japanese, 日本動物遺伝育種学会, 和光, Domestic conference

  Poster presentation


• 50K SNP チップを用いた口之島野生化牛の遺伝的多様性に関する研究

  下桐猛, 吉田彩華, 笹崎晋史, 片平清美, 大島一郎, 河邊弘太郎, 岡本新, 印牧美佐生, 国枝哲夫, 万年英之

  第15回日本動物遺伝育種学会大会, Nov. 2014, Japanese, 日本動物遺伝育種学会, 和光, Domestic conference

  Poster presentation


• The g.841G>C SNP of FASN gene is associated with fatty acid composition in beef cattle

  Hayakawa K, Ishii A, Yamaji K, Uemoto Y, Sasago N, Kobayashi E, Kobayashi N, Matsuhashi T, Maruyama S, Matsumoto H, Sasazaki S, Mannen H

  34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conference

  Oral presentation


• Paternal genetic structure of Asian goats using SRY gene

  Waki A, Sasazaki S, Kobayashi E, Mannen H

  34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conference

  Oral presentation


• LPL gene polymorphisms are associated with fatty acid composition in Japanese Black

  Koga S, Oyama K, Sasazaki S, Mannen H

  34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conference

  Oral presentation


• Genome wide association study revealed candidate regions for calving interval in Japanese black cattle

  Kawasaki Y, Fukazawa K, Fujii T, Uemoto Y, Fukushima M, Akiyama T, Kohama N, Kobayashi E, Oyama K, Sasazaki S, Mannen H

  34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conference

  Oral presentation


• Genetic diversity and relationship among 16 Asian and European cattle populations using 121 autosomal SNPs genotypes by the DigiTag2 assay

  Yonesaka R, Sasazaki S, Yasue H, Niwata S, Mukai F, Mannen H

  34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conference

  Oral presentation


• A genome wide association study for beef marbling and fatty acid composition in Japanese Black cattle using pooled DNA

  Nakajima A, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Oyama K, Sasazaki S, Mannen H

  34th International Conference on Animal Genetics, Jul. 2014, English, ISAG, Xian, China, International conference

  Oral presentation


• 黒毛和種におけるLEP遺伝子プロモーター領域に対する多型探索

  大倉 一輝, 笹崎 晋史, 大山 憲二, 万年 英之

  第14回日本動物遺伝育種学会大会, Oct. 2013, Japanese, 日本動物遺伝育種学会, 東京, Domestic conference

  Poster presentation


• mtDNA塩基配列を用いた口之島野生化牛、日本短角種、無角和種の遺伝的多様性解析

  米坂 陸, 下桐猛, 稲吉 洋裕, 印牧 美佐生, 国枝 哲夫, 向井 文雄, 笹崎 晋史, 万年 英之

  第14回日本動物遺伝育種学会大会, Oct. 2013, Japanese, 日本動物遺伝育種学会, 東京, Domestic conference

  Poster presentation


• ウシ脂肪酸組成に対するFASN 遺伝子内多型の効果

  早川 きり, 阪本 嵩幸, 石井 淳, 山地 啓太, 上本 吉伸, 笹子 奈々恵, 高須賀 晶子, 小林 栄治, 小林 直彦, 松橋 珠子, 丸山 新, 野偽 卓哉, 田淵 一郎, 平野 貴, 松本大和, 笹崎 晋史, 大山 憲二, 万年 英之

  第116回日本畜産学会大会, Mar. 2013, Japanese, 日本畜産学会, 広島, Domestic conference

  Oral presentation


• SRY遺伝子を用いたアジア在来家畜ヤギの多様性解析

  脇 愛生, 万年 英之, 笹崎 晋史

  第116回日本畜産学会大会, Mar. 2013, Japanese, 日本畜産学会, 広島, Domestic conference

  Oral presentation


• 黒毛和種の枝肉形質に対するSCD、SREBP1、FASN およびNCAPG 遺伝子多型の関連解析

  西牧 孝洋, 揖斐 隆之, 斯琴 図雅, 小林 直彦, 松橋 珠子, 今井 佳積, 松井 茉由, 上村 圭一, 衛藤 央好, 渡邉 直人, 藤田 達男, 齊藤 陽介, 小松 智彦, 万年 英之, 笹崎 晋史, 国枝 哲夫

  第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 日本動物遺伝育種学会, 仙台, Domestic conference

  Poster presentation


• 黒毛和種におけるLEP 遺伝子内多型の脂肪酸組成に対する効果

  大倉 一輝, 松本 大和, 笹崎 晋史, 大山 憲二, 万年 英之

  第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 日本動物遺伝育種学会, 仙台, Domestic conference

  Poster presentation


• 黒毛和種におけるFASN 遺伝子内多型の脂肪酸組成に対する効果

  阪本 嵩幸, 石井 淳, 山地 啓太, 早川 きり, 上本 吉伸, 笹子 奈々恵, 高須賀 晶子, 小林 栄治, 小林 直彦, 松橋 珠子, 丸山 新, 平野 貴, 杉本 喜憲, 松本 大和, 笹崎 晋史, 大山 憲二, 万年 英之

  第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 日本動物遺伝育種学会, 仙台, Domestic conference

  Poster presentation


• 牛肉中脂肪の脂肪酸組成改良に向けた新規遺伝子マーカーの探索及び効果の検証

  SASAZAKI SHINJI

  第6回家畜DNA西郷シンポジウム, Oct. 2012, Japanese, 独立行政法人家畜改良センター, Domestic conference

  Public discourse


• 岐阜県黒毛和種集団におけるUTS2R 遺伝子内多型の脂肪酸組成に対する効果

  石井 淳, 山地 啓太, 阪本 嵩幸, 秋山 賢斗, 上本 吉伸, 笹子 奈々恵, 小林 栄治, 小林 直彦, 松橋 珠子, 丸山 新, 松本 大和, 笹崎 晋史, 大山 憲二, 万年 英之

  第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 日本動物遺伝育種学会, 仙台, Domestic conference

  Poster presentation


• ウシFABP4 遺伝子のプロモーター領域に対する多型探索

  松本 大和, OYAMA KENJI, MANNEN HIDEYUKI, SASAZAKI SHINJI

  第13回日本動物遺伝育種学会大会, Oct. 2012, Japanese, 仙台, Domestic conference

  Poster presentation


• UTS2R gene polymorphisms are associated with fatty acid composition in Japanese beef cattle

  Sasazaki Shinji, Akiyama Kento, Narukami Takahiro, Matsumoto Hirokazu, Oyama Kenji, Mannen Hideyuki

  33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

  Poster presentation


• The SNPs in the ACACA gene effective on fatty acid composition in Holstein milk

  Sakamoto Takayuki, Matsumoto Hirokazu, Sasaki Kenta, Bessho Takuya, Kobayashi Eiji, Abe Tsuyoshi, Sasazaki Shinji, Oyama Kenji, Mannen Hideyuki

  33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

  Poster presentation


• The SNP in the promoter region of the bovine ELOVL5 gene is effective on subcutaneous fat thickness

  MATSUMOTO Hirokazu, SHIMIZU Yumiko, TANAKA Atsuko, NOGI Takuya, TABUCHI Ichiro, Oyama Kenji, Mannen Hideyuki, Sasazaki Shinji

  33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

  Poster presentation


• Mutations in the PLAG1 region are associated with height, weight, puberty, IGF1, and fat deposition in cattle

  Fortes M. R. S, Sasazaki Shinji, Kemper K. E, Reverter A, Pryce J, Barendse W, Bunch R, Zhang Y. D, Hawken R. J, Goddard M. E, Lehnert S. A

  33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

  Poster presentation


• Identification of quantitative trait loci affecting economic traits based on divergently selected regions between Japanese Black and Holstein cattle

  YAMAJI Keita, HOSOKAWA Daisuke, ISHII Atsushi, Sasazaki Shinji, Oyama Kenji, Mannen Hideyuki

  33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

  Poster presentation


• Genome wide association study for fatty acid composition in Japanese Black cattle

  Ishii Atsushi, Yamaji K, Uemoto Y, Kobayashi E, Sasago N, Kobayashi N, Matsuhashi T, Maruyama S, Matsumoto H, Sasazaki Shinji, Mannen Hideyuki

  33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

  Poster presentation


• Genetic diversity and structure in goat analyzed by newly-developed SNPs

  MANNEN HIDEYUKI, Lin BZ, Kato T, Kaneda M, SASAZAKI SHINJI

  33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

  Poster presentation


• Evaluation of genetic diversity in Japanese Black cattle population using SNP-MaP strategy

  Kato Taiki, Matsumoto H, Sasazaki Shinji, Akiyama T, Fukushima M, Yoshida E, Nomura T, Mannen Hideyuki

  33th International Conference on Animal Genetics, Jul. 2012, English, International Society for Animal Genetics, Cairns, Australia, International conference

  Poster presentation


• プーリング法を用いた高密度SNP解析による黒毛和種閉鎖集団の遺伝的多様性の評価

  加藤 大樹, 松本 大和, 笹崎 晋史, 秋山 敬孝, 吉田 恵実, 福島 護之, MANNEN HIDEYUKI

  第115回日本畜産学会大会, Mar. 2012, Japanese, 名古屋, Domestic conference

  Oral presentation


• 兵庫県黒毛和種肥育集団における経済形質関連遺伝子の遺伝構造

  大倉一輝, 松本大和, 秋山敬孝, 吉田恵実, 福島護之, 笹崎晋史, 万年英之

  第12回日本動物遺伝育種学会大会, Nov. 2011, Japanese, 日本動物遺伝育種学会, 広島, Domestic conference

  Poster presentation


• 黒毛和種集団を用いた脂肪酸組成に関するゲノムワイド相関解析

  石井 淳, 山地啓太, 阪本嵩幸, 上本吉伸, 笹子奈々恵, 小林栄治, 小林直彦, 松橋珠子, 丸山 新, 松本大和, 笹崎晋史, 万年英之

  第12回日本動物遺伝育種学会大会, Nov. 2011, Japanese, 日本動物遺伝育種学会, 広島, Domestic conference

  Poster presentation


• 黒毛和種およびホルスタインにおけるUTS2R遺伝子多型の脂肪酸組成に対する効果

  秋山 賢斗, 鳴神 貴太, 笹崎 晋史, 大山 憲二, 万年 英之

  第12回日本動物遺伝育種学会大会, Nov. 2011, Japanese, 日本動物遺伝育種学会, 広島, Domestic conference

  Poster presentation


• ホルスタイン集団におけるsliding window approachを利用した乳形質関連遺伝子座の探索

  山地 啓太, 石井 淳, 細川 大介, 松本 大和, 笹崎 晋史, 大山 憲二, 万年 英之

  第12回日本動物遺伝育種学会大会, Nov. 2011, Japanese, 日本動物遺伝育種学会, 広島, Domestic conference

  Poster presentation


• 黒毛和種閉鎖集団における形質関連遺伝子頻度の経時的変化

  加藤 大樹, 松本 大和, 笹崎 晋史, 秋山 敬孝, 吉田 恵実, 福島 護之, 万年 英之

  第61回関西畜産学会大会, Sep. 2011, Japanese, 関西畜産学会, 岡山, Domestic conference

  Oral presentation


• ウシELOVL5とELOVL6遺伝子のプロモーター領域に対する多型探索

  清水 裕美子, 松本 大和, 笹崎 晋史, 万年 英之

  第61回関西畜産学会大会, Sep. 2011, Japanese, 関西畜産学会, 岡山, Domestic conference

  Oral presentation


• DigiTag2 アッセイを用いたウシ個体識別及び親子鑑定システムの確立

  梶山 亮介, 原 和弘, 笹崎 晋史, 松本 大和, 安江 博, 向井 文雄, 万年 英之

  第61回関西畜産学会大会, Sep. 2011, Japanese, 関西畜産学会, 岡山, Domestic conference

  Oral presentation


• 黒毛和種集団におけるsliding window approachを利用した枝肉形質関連遺伝子座の探索

  細川 大介, 石井 淳, 山地 啓太, 笹崎 晋史, 大山 憲二, 万年 英之

  第11回日本動物遺伝育種学会大会, Oct. 2010, Japanese, 日本動物遺伝育種学会, 白河, Domestic conference

  Poster presentation


• ウシELOVL遺伝子の塩基配列決定及び多型探索

  清水 裕美子, 笹崎 晋史, 万年 英之

  第11回日本動物遺伝育種学会大会, Oct. 2010, Japanese, 日本動物遺伝育種学会, 白河, Domestic conference

  Poster presentation


• Polymorphisms of fat metabolism related genes and association between genotypes and carcass traits in Japanese Black cattle

  Narukami Takahiro, Bessyo Takuya, Tanaka Atsuko, Sasazaki Shinji, Oyama Kenji, Mannen Hideyuki

  32th International Conference on Animal Genetics, Jul. 2010, English, International Society for Animal Genetics, Edinburgh, UK, International conference

  Poster presentation


• Identification of divergently selected regions between Japanese Black and Holstein cattle based on a bovine 50k SNP array

  Yamaji Keita, Hosokawa Daisuke, Sasazaki Shinji, Mannen Hideyuki

  32th International Conference on Animal Genetics, Jul. 2010, English, International Society for Animal Genetics, Edinburgh, UK, International conference

  Poster presentation


• Genetic diversity and structure in Bos taurus and Bos indicus populations analyzed by SNP markers

  Lin BZ, Sasazaki Shinji, Mannen Hideyuki

  32th International Conference on Animal Genetics, Jul. 2010, English, International Society for Animal Genetics, Edinburgh, UK, International conference

  Poster presentation


• Development of discrimination markers between Japanese domestic and imported beef based on a bovine 50K SNP array

  Sasazaki Shinji, Hosokawa Daisuke, Ishihara Rie, Aihara Hiroki, Oyama Kenji, Mannen Hideyuki

  32th International Conference on Animal Genetics, Jul. 2010, English, International Society for Animal Genetics, Edinburgh, UK, International conference

  Poster presentation


• Allele frequencies of gene polymorphisms related to economic traits in Bos taurus and Bos indicus cattle breeds

  Kaneda Makoto, Shinji Sasazaki, Mannen Hideyuki

  32th International Conference on Animal Genetics, Jul. 2010, English, International Society for Animal Genetics, Edinburgh, UK, International conference

  Poster presentation


• 黒毛和種におけるDGAT1遺伝子多型の枝肉形質に対する効果

  鳴神 貴太, 笹崎 晋史, 大山憲二, 庄司 則章, 中嶋 宏明, 万年 英之

  第112回日本畜産学会大会, Mar. 2010, Japanese, 日本畜産学会, 明治大学, Domestic conference

  Oral presentation


• バングラデシュにおける 在来家畜及び野生原種の遺伝資源学的研究

  高橋絢子, 万年英之, 林 邦忠, 笹崎晋史, 山本義雄, 黒澤弥悦, 野村こう, 高橋幸水, 菅野雅子, Khan M.Y.A, Husain Z.M.A, Sarder M.J.U, Aziz S.A, Husain S.S, Omar Faruque MD, 天野卓

  第112回日本畜産学会大会, Mar. 2010, Japanese, 日本畜産学会, 明治大学, Domestic conference

  Oral presentation


• SNPマーカーを用いたウシの遺伝的多様性と遺伝構造解析

  林 邦忠, 笹崎晋史, 万年英之

  第112回日本畜産学会大会, Mar. 2010, Japanese, 日本畜産学会, 明治大学, Domestic conference

  Oral presentation


• Sampling method for estimating neutral allele frequency in a pedigreed population

  HONDA Takeshi, SASAZAKI Shinji, OYAMA Kenji, NOMURA Tetsuro, MUKAI Fumio

  9th World Congress of Genetics Applied to Livestock Production, 2010, English, International conference

  Poster presentation


• ホルスタイン種におけるFASN遺伝子の多型探索及び乳形質の効果

  稲田 聡士, 笹崎 晋史, 大山憲二, 真崎匡, 長谷部浩行, 阿部剛, 万年 英之

  第10回日本動物遺伝育種学会大会, Nov. 2009, Japanese, 日本動物遺伝育種学会, 群馬会館, Domestic conference

  Poster presentation


• Bovine 50k SNPs情報を利用したウシゲノム解析

  細川 大介, 笹崎 晋史, 万年 英之

  第10回日本動物遺伝育種学会大会, Nov. 2009, Japanese, 日本動物遺伝育種学会, 群馬会館, Domestic conference

  Poster presentation


• 国産牛と米国産牛とのＤＮＡ鑑定マーカーの開発

  相原 浩揮, 細川 大介, 石原 理恵, 末川 裕, 荒木 舞, 笹崎 晋史, 万年 英之

  第111回日本畜産学会大会, Sep. 2009, Japanese, 日本畜産学会, 琉球大学, Domestic conference

  Oral presentation


• ホルスタイン種における枝肉形質に関る遺伝子多型の効果

  鳴神 貴太, 笹崎 晋史, 大山 憲二, 野儀 卓哉, 万年 英之

  第111回日本畜産学会大会, Sep. 2009, Japanese, 日本畜産学会, 琉球大学, Domestic conference

  Oral presentation


• ニワトリ筋ジストロフィーにおけるcaveolin-3の発現解析

  松本 大和, 笹崎 晋史, 藤原 哲, 菊池 建機, 市原 伸恒, 万年 英之

  第111回日本畜産学会大会, Sep. 2009, Japanese, 日本畜産学会, 琉球大学, Domestic conference

  Oral presentation


• ウシ疾病関連遺伝子、抗病性関連遺伝子の多様性解析

  金田 真, 笹崎 晋史, 国枝 哲夫, 万年 英之

  第111回日本畜産学会大会, Sep. 2009, Japanese, 日本畜産学会, 琉球大学, Domestic conference

  Oral presentation


• 黒毛和種の個体識別および親子鑑定を目的としたSNPsパネルの開発

  紺 有加里, 原 和弘, 笹崎 晋史, 万年 英之

  第110回日本畜産学会大会, Mar. 2009, Japanese, 日本畜産学会, 日本大学, Domestic conference

  Oral presentation


• ウシ経済形質関連遺伝子の多様性解析

  金田 真, 笹崎 晋史, 万年 英之

  第110回日本畜産学会大会, Mar. 2009, Japanese, 日本畜産学会, 日本大学, Domestic conference

  Oral presentation

• ウシ脂肪交雑責任遺伝子ICAM1の機能解析による脂肪交雑形成機構の解明

  笹崎 晋史

  日本学術振興会, 科学研究費助成事業, 基盤研究(B), 神戸大学, 01 Apr. 2025 - 31 Mar. 2029


• Comprehensive Study of native Asian goats using whole genome information: New origins and migration routes of Southeast Asian Goats

  万年 英之, 川口 芙岐, 笹崎 晋史, 米澤 隆弘

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2024 - 31 Mar. 2028


• International joint research to elucidate the propagation of domestic animals across the Indian Ocean

  万年 英之, 米澤 隆弘, 下桐 猛, 笹崎 晋史, 川口 芙岐, 西堀 正英

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Kobe University, 07 Oct. 2021 - 31 Mar. 2026

  【研究の目的】本国際共同研究は「海の道」による家畜の海上伝播とその影響を明らかにすることを目的とし、着想したインド洋海上経路が中小家畜の伝播経路の一つであるという新しい仮説を検証する。その具体的方策として、インド洋海上経路の複数国（インドネシア、モーリシャス、マダガスカルなど）と国際共同研究を構築し、収集する中小家畜（ヤギ、ブタ、ニワトリ）試料に対し全ゲノムリシーケンスを含むゲノムワイドな遺伝学的解析を実施する。解析情報から広大なインド洋海路が家畜の伝播や遺伝子流入に与えた影響を評価し、ヒトの歴史に紐づけた家畜伝播の考察を行う。この解析には、家畜情報と試料（DNA）の収集という観点から、インド洋広範囲にわたる国際共同研究が必須となる。本課題により、インド洋海路に基づく新しい国際共同研究コンソーシアムを構築し、若手研究者参画によるグローバル視点を持つ将来の国際共同研究のリーダーを育成する。 【2021年度の研究実績】当該年度は世界的なコロナ禍が継続中のため、オンラインを使い国際共同研究者と調査計画を立案した。調査対象国はインドネシアとモーリシャスである。モーリシャスはFAREIのGobindram博士と連絡を取り、コロナ禍の状況を注視しながら2022年9月頃に2-3週間の調査を行う計画を立て、対象動物はヤギとニワトリである。モーリシャス本島とロドリゲス島の2島に対して調査を計画しつつある。インドネシアはHasanuddin大学のBugiwati 教授と連絡を取り、2022年度の調査計画を進めている。Lakor島、Timor島、Lembata島を対象とし、対象家畜はヤギである。コロナ禍の状況を鑑み、インドネシア共同研究者による試料収集を計画を進めている。採取された試料は冷凍保存し、機会を見て代表者が現地でのDNA精製等を行う予定としている。


• ウシ脂肪交雑形成機構の解明に向けた候補遺伝子変異の網羅的検証

  笹崎 晋史

  科学研究費補助金／基盤研究(C), Apr. 2022 - Mar. 2025, Principal investigator


• Have East native cattle received genetic introgression from wild cattle? -evaluation of the genetic structure and propagation route-

  万年 英之, 川口 芙岐, 笹崎 晋史, 米澤 隆弘

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2021 - 31 Mar. 2024

  本課題では、ユーラシア大陸を網羅する北方系家畜ウシ集団に対し、遺伝様式が異なるmtDNA解析、高密度SNPアレイ解析、全ゲノムリシーケンスなどによるビックデータを用いた網羅的な遺伝構造解析を実施し、その遺伝子流入、遺伝構造、起源、伝播経路などを包括的に明らかにする。その成果を通して、東アジア家畜ウシの遺伝資源保護・開発につなげ、次世代畜産分野に対する学術的知見および基礎情報を得ることを目的とする。 家畜ウシのハプログループT4は東アジア地域で特異的に観察され、特に和牛品種ではこのハプログループT4が他の東アジア地域に比べ、高頻度で観察されている。そこで本課題では、和牛品種として日本短角種と黒毛和種におけるハプログループT4のミトゲノム配列決定をすることで、ハプログループT4の系統解析や分岐年代の推定を行い、ハプログループT4の分岐・成立過程を明らかにすることを目的とした。また高密度SNPアレイを用いた解析を、和牛4品種およびアジア品種、西洋品種の遺伝子型データを得て様々な遺伝的解析を実施した。 ミトゲノム解析の結果、和牛品種は韓牛と同じクラスターに含まれ、先行研究と同様にハプログループT4はサブハプログループT3aから分岐したものであることが明らかとなった。分岐年代推定より、ハプログループT4は家畜化中心地である西アジアの肥沃な三日月地帯からシルクロードの内、温暖な環境で水が豊富に存在するオアシスの道を経て、東のアジアへと伝播する過程において生じたハプログループであると考えられた。高密度SNPアレイを用いた解析では、和牛の多様性が他の海外品種と比較して全体的に低い傾向が示された。


• 全ゲノム情報を用いたヤギの起源と伝播、環境適応形質の包括的解明に向けた先駆的研究

  万年 英之, 笹崎 晋史, 米澤 隆弘

  日本学術振興会, 科学研究費助成事業, 挑戦的研究(萌芽), 神戸大学, 28 Jun. 2019 - 31 Mar. 2023

  本研究では、世界規模で収集されたヤギのDNA試料に対し、全ゲノム解析を用いた大規模DNA情報解析を利用した起源・伝播経路の解明と環境適応形質遺伝子の全貌解明を着想し、その足掛かりとなる包括的研究を実施することを目的とした。 本年度は、mtDNAの東アジアにおける頻度分布調査とmtDNA全塩基配列決定による解析（ミトゲノム解析）、高密度SNP解析を中心に研究を進め、概ねの解析は終了した。その結果として、1) ハプログループBはパキスタン以東で高頻度に観察された。これは、ハプログループBが他のハプログループと異なる起源地を持つという先行研究を支持する結果となった。2) 12個体の東南アジア在来ヤギのミトゲノム配列の増幅に成功し、12のハプロタイプが観察された。さらにミトゲノムを使用した先行研究の配列データを用いてベイズ系統樹を作成した結果、B1が11個体、B2が1個体得られた。次に得られた系統樹を用いて、ベイズ法と最尤法で年代推定を行った結果、サブハプログループB1とB2の分岐年代は30,1600~307,700年前と推定された。3) 高密度SNP解析として、19 集団1,472 個体を本研究のデータとして使用し、14集団732個体 39,020 SNPを用いた解析を行った。系統解析からは地理的に近い集団がまとまって確認される地理的位置関係に即した結果が得られた。遺伝構造解析より、フィリピンでは東南アジア及び南アジア集団の中で唯一、スイスで優勢な遺伝的要素が確認された。また、フィリピン集団はスイスおよび南アフリカ共和国との遺伝的類似性が示唆される結果となった。一方、インドネシアでは父系統解析によりスイス・南アフリカ共和国方面からの遺伝子流入が推定されたが、核ゲノムSNP解析ではそれら地域との遺伝混合は認められなかった。


• ゲノムリシーケンスデータに基づくウシ脂肪交雑を支配する遺伝子変異の網羅的探索

  笹崎 晋史

  科学研究費補助金／基盤研究(C), Apr. 2018 - Mar. 2021, Principal investigator

  Competitive research funding


• フィリピン・インドネシア島嶼におけるマメヤギの遺伝学的調査研究

  万年 英之

  科学研究費補助金／基盤研究(B), Apr. 2017 - Mar. 2020

  Competitive research funding


• ウシ体脂肪の脂肪酸組成に関る責任遺伝子群の総合的解明

  万年 英之

  科学研究費補助金／基盤研究(B), Apr. 2016 - Mar. 2020

  Competitive research funding


• Indelマーカーの開発による家畜ウシの遺伝子流入の新たな推定法の確立と起源解明

  万年 英之

  学術研究助成基金助成金／挑戦的萌芽研究, Apr. 2016 - Mar. 2018

  Competitive research funding


• 近年におけるウシの繁殖性低下は経済形質の選抜によるものか

  万年 英之

  学術研究助成基金助成金／挑戦的萌芽研究, Apr. 2014 - Mar. 2016

  Competitive research funding


• 遺伝子発現量を基盤とした黒毛和種の不飽和脂肪酸に関与する遺伝子の探索

  大山 憲二

  科学研究費補助金／基盤研究(C), Apr. 2012 - Mar. 2015

  Competitive research funding


• 国際標準ＳＮＰシステムの構築に基づくアジア在来家畜集団の遺伝的多様性解析と保全策

  万年 英之

  科学研究費補助金／基盤研究(B), Apr. 2011 - Mar. 2014

  Competitive research funding


• ウシ脂肪酸代謝関連遺伝子の研究

  笹崎 晋史

  科学研究費補助金／若手研究(B), Apr. 2008 - Mar. 2010, Principal investigator

  Competitive research funding

• ウシ由来試料を鑑定する方法及びキット

  MANNEN HIDEYUKI, SASAZAKI SHINJI

  特願2010-095158, 16 Apr. 2010, 大学長, 特許5800307, 04 Sep. 2015

  Patent right


• 非黒毛和種か否かを鑑定する方法及びキット

  MANNEN HIDEYUKI, SASAZAKI SHINJI

  特願2009-223172, 28 Sep. 2009, 大学長, 特許5729897, 17 Apr. 2015

  Patent right


• 国産牛と豪州産輸入牛の鑑別方法

  MANNEN HIDEYUKI, SASAZAKI SHINJI, 向井 文雄

  特願2006-224984, 22 Aug. 2006, 大学長, 特許4872084, 02 Dec. 2011

  Patent right