Research activity information

• Mar. 2022 公益財団法人日本農芸化学会, JSBBA Award for Young Investigators, 難吸収性食品成分の組織間ネットワークを介した生理機能に関する研究


• Jul. 2021 Japan Society of Nutrition and Food Science, JSNFS Award for Young Investigators, ポリフェノールの消化管シグナルを介した生体調節機能解明に関する研究


• Nov. 2020 公益財団法人農学会, 農学進歩賞, 難吸収食品成分の機能性に関する時空間的制御機構の解明


• Jan. 2019 Kobe university, 神戸大学 平成３０年度優秀若手研究者賞, プロシアニジンの肥満高血糖予防効果

  ヤマシタヨウコ

  Others


• Mar. 2018 日本農芸化学会, JSBBA Award for Young Women Scientists, プロシアニジンの高血糖・肥満予防効果に関する研究

  YAMASHITA YOKO

  Japan society


• Dec. 2014 一般財団法人大阪科学技術センター, 第3回ネイチャー・インダストリー・アワード特別賞, メタボリックシンドロームの予防・改善に寄与する機能性成分の網羅的探索法と作用機構解明，ならびにそれを高含有する農作物生産のための環境創造型農業の開発

  YAMASHITA Yoko, ASHIDA HITOSHI, 保田 茂

  Japan society


• Nov. 2014 日本フードファクター学会, Young Investigator Award 第1位, 若手研究者

  YAMASHITA YOKO

  Japan society


• 2013 公益社団法人日本食品科学工学会, 第60回日本食品科学工学会 優秀発表賞, 重合度の異なるカカオ由来プロシアニジンが筋肉細胞でのGLUT4膜移行を介したグルコース取り込みにおよぼす効果

  YAMASHITA YOKO

  Japan society


• 2013 日本薬学会生薬天然物部会, 第5回食品薬学シンポジウム 優秀発表賞, カカオポリフェノールの高血糖・肥満抑制効果とその作用機構

  YAMASHITA YOKO, ASHIDA HITOSHI

  Japan society


• 2013 日本フードファクター学会, 第18回日本フードファクター学会 学術集会(JSoFF2013) Young investigator award銅賞, カカオ由来プロシアニジンによるインクレチン効果を介した血糖調整作用

  YAMASHITA YOKO

  Japan society


• Sep. 2011 日本カテキン学会, 最優秀プレゼンテーション賞, 若手研究者

  YAMASHITA YOKO

  Japan society


• 2011 日本フードファクター学会, JSoFF travel award, エピカテキン重合体であるプロシアニジンを高含有する組成物の肥満予防効果

  YOKO Yamashita

  International society

• Theaflavin 3'-gallate activates G protein-coupled receptor 55 (GPR55) and enhances GLP-1 secretion via Ca2+/CaMKII/ERK signaling in enteroendocrine STC-1 cells, mitigating postprandial hyperglycemia in mice.

  Kevin Odongo, Naoki Harada, Ryoichi Yamaji, Yoko Yamashita, Hitoshi Ashida

  The antihyperglycemic effect of black tea is well-known, and theaflavins (TFs) are considered active compounds. It is, however, unclear whether glucagon-like peptide-1 (GLP-1) is involved in the antihyperglycemic effects of TFs. We demonstrate that TFs suppress postprandial hyperglycemia by stimulating GLP-1 secretion in mice. In STC-1 cells, theaflavin 3'-gallate (TF2B), possessing a galloyl group at the 3'-position, showed the strongest effect on GLP-1 secretion among the four TFs. TF2B activated G protein-coupled receptor 55 (GPR55) and was confirmed to bind to the receptor, notably exhibiting the highest binding affinity. Moreover, GPR55 antagonist canceled TF2B-induced GLP-1 secretion. Downstream, TF2B increased intracellular Ca2+ levels and activated the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and extracellular signal-regulated kinases 1/2 (ERK1/2) pathways. Inhibitors of Ca2+ signaling, CaMKII, and ERK pathways abolished TF2B-stimulated GLP-1 secretion. These findings suggest that TF2B from black tea prevents hyperglycemia through GPR55-dependent stimulation of GLP-1 secretion via Ca2+-Ca2+/CaMKII and ERK1/2 pathways.

  Mar. 2025, Food & function, 16(6) (6), 2487 - 2502, English, International magazine

  Scientific journal


• Ashitaba Chalcone 4-Hydroxydericcin Promotes Glucagon-Like Peptide-1 Secretion and Prevents Postprandial Hyperglycemia in Mice.

  Kevin Odongo, Moe Ishinaka, Ayane Abe, Naoki Harada, Ryoichi Yamaji, Yoko Yamashita, Hitoshi Ashida

  Certain polyphenols improve glucose tolerance by stimulating glucagon-like peptide-1 (GLP-1) secretion from intestinal L-cells. Ashitaba chalcones, 4-hydroxyderricin (4-HD), and xanthoangelol (XAG) have antihyperglycemic effects, but their molecular mechanism, including whether they promote GLP-1 secretion is unknown. This study investigates the 4-HD-induced GLP-1 secretory mechanisms and its anti-hyperglycemic effects. The secretory mechanisms were examined in STC-1 cells and antihyperglycemic effects in male ICR mice. In STC-1 cells, 4-HD, but not XAG, stimulated GLP-1 secretion through membrane depolarization and intracellular Ca2+ increase [Ca2+]i, via the L-type Ca2+ channel (VGCC). Verapamil and nifedipine, blockers of VGCC, and treatment in Ca2+-free buffer abolished 4-HD effects on [Ca2+]i and GLP-1 secretion. Moreover, 4-HD activated CaMKII and ERK1/2. Consistently, oral 4-HD suppressed postprandial hyperglycemia in mice and increased plasma GLP-1 and insulin levels, GLUT4 translocation, and activation of LKB-1 and Akt pathways in skeletal muscle. Furthermore, exendin 9-39, a GLP-1R antagonist, and compound C, an AMPK inhibitor, completely canceled the 4-HD-caused anti-hyperglycemic activities. 4-HD stimulated GLP-1 secretion through membrane depolarization coupled with [Ca2+]i increase via VGCC in L-cells and activated AMPK- and insulin-induced GLUT4 translocation in skeletal muscle. Thus, 4-HD possesses dual mechanisms for the prevention of hyperglycemia.

  Mar. 2025, Molecular nutrition & food research, 69(6) (6), e202400690, English, International magazine

  Scientific journal


• Black Soybean Seed Coat Extract Suppresses Gut Tumorigenesis by Augmenting the Production of Gut Microbiota-Derived Short-Chain Fatty Acids.

  Yasuyuki Shimizu, Shunta Hirano, Mohammed Salah, Namiko Hoshi, Yoko Yamashita, Takeshi Fukumoto, Naritoshi Mukumoto, Ai Nakaoka, Takeaki Ishihara, Daisuke Miyawaki, Hitoshi Ashida, Ryohei Sasaki

  BACKGROUND: Proanthocyanidins (PACs) from black soybean seed coat have antioxidant and anti-tumorigenic properties. We investigated the anti-tumor properties and mechanisms of action of PACs on colorectal cancer (CRC). METHODS: We fed the APCmin/+ mice, which are highly susceptible to spontaneous intestinal adenoma formation, diets supplemented with or without PACs for 7 weeks and assessed adverse effects, the number and size of intestinal polyps, and the expression of pro- and anti-proliferative proteins in the intestine. The mouse gut microbiome composition was analyzed, and the concentrations of gut short-chain fatty acids (SCFAs) were quantified. We also compared CRC incidence in Tamba in Japan, where black soybean is consumed frequently, with that in the rest of Japan. RESULTS: The number and size of intestinal polyps notably decreased in the PAC-fed mice. Compared with control mice, the PAC-fed mice showed lower expression of proliferation markers proliferating cell nuclear antigen and β catenin and a higher expression of the anti-inflammatory protein oligomeric mucus gel-forming. PAC supplementation increased the prevalence and concentrations of beneficial gut microbes and SCFAs, respectively. CONCLUSIONS: Diet supplemented with black soybean-derived PACs could prevent CRC development in mice through gut microbiome remodeling. Regions consuming black soybeans have low CRC incidence. Notably, the incidence of CRC, breast cancer, and liver cancer was significantly lower in Tamba than in the rest of Hyogo Prefecture or Japan. Future studies should delineate the mechanisms underlying the CRC-protective effects of PACs. Nevertheless, our results demonstrate the potential of including PACs in dietary recommendations for cancer prevention.

  Nov. 2024, Cancers, 16(22) (22), English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A Natural Chalcone Cardamonin Inhibited Transformation of Aryl Hydrocarbon Receptor Through Binding to the Receptor Competitively.

  Tianshun Zhang, Chao He, Shieru Ota, Tomoya Kitakaze, Ryoichi Yamaji, Sayuri Shimazu, Yoko Yamashita, Hitoshi Ashida

  SCOPE: Chalcones are widely present in most plants and have various health beneficial functions. This study investigates the suppressive effect of 13 natural and synthetic chalcones on transformation of aryl hydrocarbon receptor (AhR) induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 3-methylcholanthrene (3-MC) in a cell-free system, Hepa-1c1c7 cells, and liver of ICR mice. METHODS AND RESULTS: In the cell-free system, cardamonin dose-dependently inhibits AhR transformation. Chalcones with substitution on 2' and/or 6' position is important for the suppressive effect, while the substitution on 4' position is negatively for the effect. Moreover, cardamonin and 2'-hydroxychalcone competitively inhibit the binding of [3H]-3-MC to the AhR. In Hepa-1c1c7 cells, cardamonin inhibits AhR transformation and expression of cytochrome P4501A1 (CYP1A1) in a dose-dependent manner through suppressing TCDD-induced phosphorylation of both AhR and AhR nuclear translocator, heterodimerization of them, and nuclear translocation of AhR. In the liver of mice, oral administered cardamonin also inhibits 3-MC-induced AhR translocation and expression of CYP1A1. CONCLUSION: Among used chalcones, a natural chalcone cardamonin competitively binds to AhR and suppresses its transformation. Thus, cardamonin is an effective food factor for suppression of the dioxin-caused biochemical alterations and toxicities.

  Sep. 2024, Molecular nutrition & food research, 68(18) (18), e2400185, English, International magazine

  Scientific journal


• Dietary flaxseed oil suppresses hyperglycemia and hyperinsulinemia through increasing in α-linolenic acid content in the muscle.

  Midori Seike, Yasuko Makino, Yoko Yamashita, Hitoshi Ashida

  Types of fats and oils affect the onset of lifestyle diseases. In this study, we investigated the relationship between the postprandial hyperglycemia and fatty acids content in the skeletal muscle of C57BL/6 mice given 20% lard, palm oil, corn oil, safflower oil, and flaxseed oil for 16 weeks. Lard increased plasma glucose and insulin levels at the end of feeding period, whereas flaxseed oil did not. It was noteworthy that there is a positive correlation between palmitic acid content in the muscle and postprandial hyperglycemia, and a negative correlation between α-linolenic acid content and hyperglycemia. Alternatively, mice were given 30% lard for 16 weeks. When lard was partially substituted with flaxseed oil (10-50% substitution), flaxseed oil dose-dependently prevented lard-induced hyperglycemia and hyperinsulinemia. In conclusion, flaxseed oil prevents the adverse effects of lard through increasing in α-linolenic acid content in the muscle.

  Sep. 2024, Journal of clinical biochemistry and nutrition, 75(2) (2), 133 - 144, English, Domestic magazine

  Scientific journal


• Physiological functions of poorly absorbed polyphenols via the glucagon-like peptide 1.

  Yoko Yamashita

  Polyphenols are compounds of plant origin with several documented bioactivities related to health promotion. Some polyphenols are hard to be absorbed into the body due to their structural characteristics. This review focuses on the health beneficial effects of polyphenols mediated by intestinal hormones, particularly related to the systemic functions through the secretion of glucagon-like peptide 1 (GLP-1), an enteric hormone that stimulates postprandial insulin secretion. GLP-1 is secreted from L cells in the distal small intestine. Therefore, some poorly absorbed polyphenols are known to have the ability to act on the intestines and promote GLP-1 secretion. It has been reported that it not only reduces hyperglycemia but also prevents obesity by reduction of overeating and improves blood vessel function. In this review discuss examples of health effects of polyphenols mediated by GLP-1 secretion.

  Feb. 2024, Bioscience, biotechnology, and biochemistry, 88(5) (5), 493 - 498, English, International magazine

  Scientific journal


• Black Soybean Seed Coat Extract Improves Endothelial Function and Upregulates Oxidative Stress Marker Expression in Healthy Volunteers by Stimulating Nitric Oxide Production in Endothelial Cells.

  Ryota Akagi, Fumio Nanba, Shizuka Saito, Toshinari Maruo, Toshiya Toda, Yoko Yamashita, Hitoshi Ashida, Toshio Suzuki

  Black soybean seed coat extract (BE) contains multiple bioactive polyphenols, including flavan-3-ols and anthocyanins. BE improves endothelial function; however, it is unclear whether BE protects endothelial cells from senescence. In this study, we examined the effects of BE on endothelial cell senescence and vascular function in healthy individuals. High concentrations of glucose were used to induce senescence in bovine aortic endothelial cells incubated with BE. Senescence, vascular function, and oxidative stress markers were measured. Incubation with BE remarkably inhibited senescence-associated β-galactosidase and lactate dehydrogenase activities and dose dependently reduced intracellular reactive oxygen species levels in bovine aortic endothelial cells. BE treatment increased the levels of endothelial nitric oxide synthase (eNOS) mRNA and endothelial nitric oxide (NO) metabolites and increased the mRNA expression of klotho, a gene associated with an antiaging phenotype. To examine the effects of BE in humans, we conducted a clinical study using the second derivative of the fingertip photoplethysmogram to investigate vascular function and aging in 24 healthy volunteers. The participants consumed BE supplements (100 mg/day) or a placebo for 2 weeks. When compared with the placebo group, the BE group showed considerably improved vascular function, NO metabolite levels, and oxidative stress. These results suggest that BE supplementation improves endothelial function, possibly through antioxidant activity and NO production, and may consequently reduce the cardiovascular risk associated with aging. BE supplementation may be an effective and safe approach to reduce the risk of atherosclerosis and cardiovascular disease; however, additional studies investigating chronic vascular inflammation are needed.

  Feb. 2024, Journal of medicinal food, 27(2) (2), 134 - 144, English, International magazine

  Scientific journal


• Black soybean seed coat polyphenols have different effects on glucose and lipid metabolism in growing and young adult mice.

  Toshiki Nishijima, Yoko Yamashita, Hitoshi Ashida

  Black soybean contains flavan-3-ols and cyanidin 3-O-glucoside in its seed coat. Polyphenol-rich black soybean seed coat extract (BE) possesses various health benefits, such as antioxidant, anti-obesity, and anti-hyperglycemic effects. However, these functions have been evaluated mainly in the growing stage of animals, and there is no comparison data for different life stages. In this present study, we compared the effect of BE in growing (5-week old) and young adult (22-week old) ICR male mice. These mice were given an AIN 93M diet containing 2.0% BE for 4 weeks. BE did not affect body weight gain in both growing and young adult mice, but it suppressed mesenteric and subcutaneous white adipose tissue weights and decreased the cell size. BE also significantly suppressed plasma free-fatty acid levels. The effect of both BE and life stages were observed in the protein expression of adipogenesis-related transcription factors; in particular, BE suppressed the expression of C/EBPα and PPARγ. No significant change was observed in lipolysis and lipogenesis factors in the white adipose tissue and liver. Alternatively, BE showed low glucose tolerance without affecting plasma insulin levels after glucose loading in young adult mice, as seen from the results of the oral glucose tolerance test. However, plasma glucose and insulin levels remained unchanged at the end of the experimental period. In conclusion, these results strongly suggest that the health-beneficial effects of BE may alter in mice at different life stages.

  Jan. 2024, Food & function, 15(2) (2), 1004 - 1020, English, International magazine

  Scientific journal


• Polyphenol content and antioxidant activity of two black soybean varieties over five cultivation years

  Wei-Jing Li, Kevin Odongo, Ken-yu Hironao, Yoko Yamashita, Hitoshi Ashida

  Japanese Society for Food Science and Technology, 2024, Food Science and Technology Research, 30(3) (3), 367 - 376

  [Refereed]

  Scientific journal


• Dietary flaxseed oil induces production of adiponectin in visceral fat and prevents obesity in mice.

  Midori Seike, Hitoshi Ashida, Yoko Yamashita

  Induction of obesity by dietary fats and oils differs according to the type of fat. Adiponectin is believed to be related to obesity prevention. We hypothesized that flaxseed oil is important for preventing obesity and producing adiponectin. To clarify this hypothesis, we investigated the relationship between obesity and different fat sources in mice fed diets with 6 types of fat and oils. C57BL/6J mice were given a control diet containing 5% corn oil or a high-fat diet containing 20% of either lard, palm oil, rapeseed oil, oleate-rich safflower oil, corn oil, or flaxseed oil for 14 weeks. In another experiment, mice were given a control diet and rosiglitazone (10 mg/kg body weight) by oral gavage for 1 week. At the end of study, plasma adiponectin and expression of fatty acid metabolism-related factors in white and brown adipose tissue and the liver were measured. Dietary flaxseed oil, which is rich in α-linolenic acid, did not induce obesity. Flaxseed oil resulted in increased β-oxidation-related factors in epididymal white adipose tissue, decreased fatty acid synthesis-related factors in the liver, and thermogenesis-related factor in brown adipose tissue following increase of plasma adiponectin. The results suggested that increase in plasma adiponectin after intake of flaxseed oil may be due to altered expression of AdipoQ and peroxisome proliferator-activated receptor γ in epididymal white adipose tissue. Flaxseed oil increased expression of adiponectin in visceral fat and regulated obesity-controlling fatty acid metabolism-related factors in white adipose tissue and liver, and thermogenesis-related factor in brown adipose tissue.

  Jan. 2024, Nutrition research (New York, N.Y.), 121, 16 - 27, English, International magazine

  Scientific journal


• Single oral administration of quercetin glycosides prevented acute hyperglycemia by promoting GLUT4 translocation in skeletal muscles through the activation of AMPK in mice.

  Yoko Yamashita, Hao Jiang, Fukiko Okada, Tomoya Kitakaze, Yasukiyo Yoshioka, Hitoshi Ashida

  Quercetin is a natural flavonol and has various health beneficial functions. Our pervious study demonstrated that long-term feeding (13 weeks) of quercetin and its glycosides, isoquercitrin, rutin, and enzymatically modified isoquercitrin, which is a mixture of quercetin monoglycoside and its oligoglycosides, prevented hyperglycemia and adiposity in mice fed a high-fat diet but not standard diet. It is, however, unclear whether a single administration of these compounds prevent postprandial hyperglycemia or not. In the present study, we estimated their prevention effect on acute hyperglycemia by an oral glucose tolerance test in ICR mice and investigated its mechanism. It was found that quercetin glycosides, but not the aglycone, suppressed acute hyperglycemia and isoquercitrin showed the strongest effect among the glycosides. As the underlying mechanism, quercetin glycosides promoted translocation of glucose transporter 4 to the plasma membrane of skeletal muscle of mice through phosphorylation of adenosine monophosphate-activated protein kinase and its upstream Ca2+/calmodulin-dependent protein kinase kinase β without activating the insulin- and JAK/STAT-signal pathways. In conclusion, single oral administration of quercetin glycosides prevented a blood sugar spike by promoting glucose transporter 4 translocation through activating the CAMKKβ/AMPK signaling pathway.

  Jan. 2024, Journal of clinical biochemistry and nutrition, 74(1) (1), 37 - 46, English, Domestic magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Mung bean peptides promote glucose uptake via Jak2 activation in L6 myotubes.

  Yasukiyo Yoshioka, Qing Zhang, Xin Wang, Tomoya Kitakaze, Yoko Yamashita, Mitsutaka Kohno, Hitoshi Ashida

  Mung beans are among the important edible legumes cultivated in Asia, Southern Europe, and Northern America. Mung beans contain 20-30% proteins with high digestibility and possess biological activities, but detailed health beneficial functions are not fully understood yet. In this study, we report the isolation and identification of active peptides from mung beans which promote glucose uptake and elucidate their mechanism in L6 myotubes. HTL, FLSSTEAQQSY, and TLVNPDGRDSY were isolated and identified as active peptides. These peptides promoted the translocation of glucose transporter 4 (GLUT4) to the plasma membrane. The tripeptide HTL promoted glucose uptake through the activation of adenosine monophosphate-activated protein kinase, while the oligopeptides FLSSTEAQQSY and TLVNPDGRDSY through the activation of the PI3K/Akt pathway. Furthermore, these peptides promoted the phosphorylation of Jak2 via interaction with the leptin receptor. Thus, mung bean is a promising functional food for the prevention of hyperglycemia and type 2 diabetes through promoting glucose uptake accompanied by JAK2 activation in the muscle cells.

  May 2023, Food & function, English, International magazine

  Scientific journal


• Development of sandwich ELISAs for detecting glucagon-like peptide-1 secretion from intestinal L-cells and their application in STC-1 cells and mice.

  Kevin Odongo, Ken-Yu Hironao, Yoko Yamashita, Hitoshi Ashida

  Certain nutrients stimulate glucagon-like peptide-1 (GLP-1) secretion from the intestinal enteroendocrine L-cells, but due to rapid degradation by the DPP-4 enzyme, >90% is converted to inactive metabolite before reaching the target organs via circulation. Plants are a source of potent bioactive compounds that promote endogenous secretion of GLP-1 from L-cells. To search for the effective bioactive compound from a vast library of natural compounds, a reliable and low-cost assay is required considering the high cost of commercial assays. We developed a low-cost sandwich enzyme-linked immunosorbent assays (s-ELISAs) for detecting 'total', 'sensitive active', and 'wide-range active' GLP-1. The s-ELISAs exhibited high sensitivity with measurement ranges between 0.94~240, 0.98~62.5, and 4.8~4,480 pmol/L, respectively. High precision was observed; i.e., CVs within 5% and 20% for intra- and inter-assay variations, respectively, and excellent recovery of exogenous GLP-1 from assay buffer. The developed s-ELISAs had the same performance as the commercial kits and approximately 80% cheaper cost. For their application, cinnamtannin A2-induced GLP-1 secretion was confirmed in STC-1 cells consistent with our previous findings. The s-ELISAs were further validated by measuring plasma GLP-1 level in mice after oral administration of black soy bean seed coat extract containing cinnamtannin A2.

  Jan. 2023, Journal of clinical biochemistry and nutrition, 72(1) (1), 28 - 38, English, Domestic magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Pectolinarigenin Induces Antioxidant Enzymes through Nrf2/ARE Pathway in HepG2 Cells.

  Mariko Shiraiwa, Tomoya Kitakaze, Yoko Yamashita, Yuichi Ukawa, Katsuyuki Mukai, Hitoshi Ashida

  Pectolinarigenin (PG) and its glycoside pectolinarin (PN) were reported to have various health beneficial functions such as anti-inflammatory and anti-carcinogenic activities. It has also been reported that PG and PN have radical scavenging ability as direct antioxidant activity. However, the indirect antioxidant activity of PG and PN by inducing antioxidant enzymes in hepatocytes is not fully understood yet. In this study, we investigated whether PG and PN increase expression of antioxidant enzymes through the nuclear factor-erythroid-2-related factor 2 (Nrf2)-mediated pathway in human hepatoma HepG2 cells and the liver of male ICR mice. PG, but not PN, induced antioxidant enzymes, namely heme oxigenase-1, NAD(P)H:quinone oxidoreductase 1, and aldo-keto reductase family 1 member B10, in HepG2 cells. As for the induction mechanism of these enzymes, PG-induced nuclear accumulation of Nrf2 increased antioxidant response element (ARE)-mediated transcriptional activity and suppressed degradation of Nrf2 through modification of Kelch-like EXH-associated protein 1. Oral administration of PG also induced nuclear accumulation Nrf2 and expression of antioxidant enzymes in the liver of mice. Therefore, PG, but not PN, exhibits the indirect antioxidant activity by inducing antioxidant enzymes through the Nrf2/ARE pathway and may protect liver from oxidative stress.

  Mar. 2022, Antioxidants (Basel, Switzerland), 11(4) (4), English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Phenylpropanoids and neolignans isolated from Myristica fragrans enhance glucose uptake in myotubes.

  Yasukiyo Yoshioka, Ryunoshin Kono, Masaki Kuse, Yoko Yamashita, Hitoshi Ashida

  Nutmeg is the seed of Myristica fragrans or its powder and is used as a spice and a traditional medicine. The antidiabetic effect of nutmeg is not fully understood yet. In this study, we examine the isolation and identification of the active compounds of Myristica fragrans with regards to glucose uptake and elucidate their mechanism in L6 myotubes. Myrisiticin, licarin B, erythro-2-(4-allyl-2,6-dimethoxy-phenoxy)-1-(3,4-dimethoxyphenyl)-propan-1-ol (ADDP) and (7S,8R)-2-(4-allyl-2,6-dimethoxyphenoxy)-1-(3,4,5-trimethoxyphenyl)-propan-1-ol (ADTP) were isolated and identified as the active compounds. Myristicin or a mixture of ADDP and ADTP promoted the translocation of glucose transporter 4 (GLUT4) through phosphorylation of AMP-activated protein kinase in L6 myotubes 15 min after treatment, while licarin B promoted it 240 min after treatment. Oral administration of the fraction from Myristica fragrans containing these active compounds to ICR mice suppressed post-prandial hyperglycemia. Thus, Myristica fragrans is a promising functional food to prevent post-prandial hyperglycemia and type 2 diabetes mellitus by promoting glucose uptake in muscle.

  Mar. 2022, Food & function, English, International magazine

  [Refereed]

  Scientific journal


• Preventive effects of black soybean polyphenols on non-alcoholic fatty liver disease in three different mouse models.

  Mio Yamamoto, Yasukiyo Yoshioka, Tomoya Kitakaze, Yoko Yamashita, Hitoshi Ashida

  Non-alcoholic fatty liver disease (NAFLD) and its advanced stage, non-alcoholic steatohepatitis (NASH), are a major health issue throughout the world. Certain food components such as polyphenols are expected to possess preventive effects on NAFLD and NASH. In this study, the preventive effects of black soybean polyphenols were examined by using three NAFLD/NASH animal models. In a choline-deficient and L-amino acid-defined high-fat diet-induced NASH model, the intake of black soybean polyphenols decreased oxidative stress, but failed in attenuating liver injury and decreasing the expression of alpha-smooth muscle actin (α-SMA). In a Western diet with sucrose and fructose containing sweetened water-induced NAFLD model, black soybean polyphenols suppressed hepatic lipid accumulation, oxidative stress, aminotransferase activities in the plasma, inflammatory cytokine expression, and α-SMA expression accompanied by modulation of lipid metabolism. In a combination of Western diet and carbon tetrachloride model, black soybean polyphenols also suppressed hepatic lipid accumulation, oxidative stress, aminotransferase activities in the plasma, and α-SMA expression. In conclusion, black soybean is an attractive food for the prevention of NAFLD and NASH due to its strong antioxidant activity.

  Jan. 2022, Food & function, 13(2) (2), 1000 - 1014, English, International magazine

  [Refereed]

  Scientific journal


• Black soybean seed coat polyphenol ameliorates the abnormal feeding pattern induced by high-fat diet consumption.

  Ken-Yu Hironao, Hitoshi Ashida, Yoko Yamashita

  High-fat diet (HFD) consumption induces chronic inflammation and microglial accumulation in the mediobasal hypothalamus (MBH), the central regulator of feeding behavior and peripheral metabolism. As a result, the diurnal feeding rhythm is disrupted, leading to the development of obesity. Diet-induced obesity (DIO) can be prevented by restoring the normal feeding pattern. Therefore, functional foods and drugs that ameliorate hypothalamic inflammation and restore the normal feeding pattern may prevent or ameliorate DIO. Numerous functional foods and food-derived compounds with anti-obesity effects have been identified; however, few studies have been performed that assessed their potential to prevent the HFD-induced hypothalamic inflammation and disruption of feeding rhythm. In the present study, we found that polyphenols derived from black soybean seed coat (BE) significantly ameliorated the accumulation of activated microglia and pro-inflammatory cytokine expression in the arcuate nucleus of the hypothalamus of HFD-fed mice, and restored their feeding pattern to one comparable to that of standard diet-fed mice, thereby ameliorating DIO. Furthermore, cyanidin 3-O-glucoside-the principal anthocyanin in BE-was found to be a strong candidate mediator of these effects. This is the first study to show that BE has the potential to provide a variety of beneficial effects on health, which involve amelioration of the HFD-induced hypothalamic inflammation and abnormal feeding pattern. The results of this study provide new evidence for the anti-obesity effects of black soybean polyphenols.

  2022, Frontiers in nutrition, 9, 1006132 - 1006132, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 5-Aminolevulinic acid combined with ferrous iron improves glucose tolerance in high-fat diet-fed mice via upregulation of glucose transporter 1.

  Yasushi Kuroda, Atsuko Kamiya, Takuya Ishii, Masahiro Ishizuka, Yoko Yamashita, Hitoshi Ashida

  Decreased mitochondrial metabolism suppresses glucose metabolism, resulting in obesity and diabetes. The present study aimed to investigate mechanisms underlying the 5-aminolevulinic acid (5-ALA) hydrochloride-mediated increase in glucose uptake in high-fat diet (HFD)-fed mice in vivo and C2C12 myotube cells in vitro. C57BL/6N male mice (20 weeks old) were fed either HFD or normal diet (ND) for 4 weeks. A total of five HFD-fed mice were orally administered with 300 mg/kg 5-ALA hydrochloride and 47.1 mg/kg sodium ferrous citrate (SFC; HFD + 5-ALA/SFC), whereas ND and other HFD-fed mice were orally administered with saline. After 4 weeks, these mice were intraperitoneally administered with 2 g/kg glucose and 3.2 mg/kg 2-deoxyglucose (2DG) for intraperitoneal glucose tolerance test (IPGTT) and glucose uptake test. Body weights, plasma glucose levels and the area under the curve of IPGTT were lower in mice treated with HFD + 5-ALA/SFC compared with in those treated with HFD alone. 2DG uptake in the gastrocnemius muscle and heart were more significantly improved in the HFD + 5-ALA/SFC mice compared with the HFD-fed mice. Furthermore, 5-ALA/SFC increased 2DG uptake in C2C12 cells to a similar level to the insulin-treated group. Moreover, it increased glucose transport (GLUT)1 translocation in the plasma membrane by 2.5-fold relative to the controls without affecting GLUT1 expression; however, it had no effect on GLUT4 translocation. Therefore, 5-ALA/SFC enhanced gastrocnemius and cardiac glucose uptake in HFD-fed mice, and upregulated GLUT1 translocation to the plasma membrane, but not GLUT4 in C2C12 myotube cells. Therefore, it could potentially be used as a novel drug for the treatment of diabetes.

  Dec. 2021, Experimental and therapeutic medicine, 22(6) (6), 1454 - 1454, English, International magazine

  [Refereed]

  Scientific journal


• Kaempferol Promotes Glucose Uptake in Myotubes through a JAK2-Dependent Pathway.

  Tomoya Kitakaze, Hao Jiang, Takuya Nomura, Ken-Yu Hironao, Yoko Yamashita, Hitoshi Ashida

  Kaempferol possesses various health-promoting functions including antihyperglycemic activity, but its underlying molecular mechanism is poorly understood. Glucose transporter 4 (GLUT4) plays an important role in the uptake of blood glucose into muscle cells after its translocation to the plasma membrane. In this study, we demonstrated that kaempferol at 1.0 nM or more significantly increased the uptake of 2-[3H]- deoxy-d-glucose by 1.3-1.4-fold in L6 myotubes. Kaempferol at 10 pM or more also significantly increased GLUT4 translocation by 1.3-1.6-fold. Kaempferol at 1.0 nM significantly increased the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) by 2.9-fold, liver kinase B1 and Janus kinase 2 (JAK2) by 1.9-fold, and signal transducer and activator of transcription 3 by 3.7-fold. In addition, kaempferol increased phosphorylation of phosphoinositide 3-kinase (PI3K) by 1.8-fold but not the insulin receptor. Small interfering RNA (siRNA) for AMPK, JAK2, or PI3K canceled kaempferol-induced glucose uptake and GLUT4 translocation. Furthermore, siRNA for JAK2 canceled kaempferol-induced phosphorylation of AMPK and PI3K. These results indicate that a JAK2-depdendent pathway regulates kaempferol-induced glucose uptake and GLUT4 translocation in L6 myotubes and that kaempferol may be an effective compound for the prevention of hyperglycemia.

  Nov. 2020, Journal of agricultural and food chemistry, 68(47) (47), 13720 - 13729, English, International magazine

  [Refereed]

  Scientific journal


• Insights into the potential benefits of black soybean (Glycine max L.) polyphenols in lifestyle diseases.

  Yoko Yamashita, Hiroyuki Sakakibara, Toshiya Toda, Hitoshi Ashida

  Black soybean (Glycine max L.), a cultivar containing abundant polyphenols in its seed coat such as anthocyanins and flavan-3-ols, has been reported to possess various health benefits toward lifestyle diseases. In this review article, the safety evaluation of polyphenol-rich black soybean seed coat extract (BE) and absorption of BE polyphenols are summarized. Additionally, we describe the antioxidant activity of BE polyphenols and their ability to induce antioxidant enzymes. The health benefits of BE and its polyphenols, such as anti-obesity and anti-hyperglycemic activities through the activation of AMP-activated protein kinase and translocation of glucose transporter 4, respectively, are also discussed. Furthermore, we found that black soybean polyphenols were involved in the improvement of vascular function. These emerging data require further investigation in scientific studies and human trials to evaluate the prevention of lifestyle diseases using black soybean polyphenols.

  Sep. 2020, Food & function, 11(9) (9), 7321 - 7339, English, International magazine

  [Refereed]

  Scientific journal


• Black Soybean Improves Vascular Function and Blood Pressure: A Randomized, Placebo Controlled, Crossover Trial in Humans.

  Yoko Yamashita, Asuka Nakamura, Fumio Nanba, Shizuka Saito, Toshiya Toda, Junichi Nakagawa, Hitoshi Ashida

  Vascular dysfunction and injurious stimuli such as oxidative stress are closely related to the risk of cardiovascular diseases (CVD). Dietary polyphenols are reported to exert beneficial effects in reducing the risk of CVD. Black soybean has been used as a nutritionally rich food and contains abundant polyphenols in its seed coat and grain. Black soybean has many beneficial physiological activities, and its prevention effects on CVD risk were reported mainly in animal experiments. In this study, we performed a randomized, single blind, placebo controlled, crossover trial to investigate the effect of black soybean consumption on the vascular function in healthy humans. Twenty-two healthy adults aged from 30 to 60 completed the four week trial with daily consumption of about a 40 g test material cookie containing 20 g roasted black soybean powder. Body composition, vascular function, biomarkers for oxidative stress, and polyphenol contents in the urine and the plasma were measured. After ingestion of the black soybean cookie, vascular function, which was evaluated by plethysmogram using a Pulse Analyzer®, was improved and systolic blood pressure was decreased. Moreover, nitric oxide levels in plasma and urine were increased, while an oxidative stress biomarker, 8-hydroxy-2'-deoxyguanosine level, in the plasma was decreased accompanied by an increase in the concentration of polyphenols derived from black soybean in plasma and urine. These results suggest that the antioxidant activity of black soybean polyphenols and an increase in the nitric oxide level may contribute to the improvement of vascular function. Thus, black soybean is an attractive food material for improvement of vascular function through decreasing oxidative stress by its potent antioxidant activity and increasing the nitric oxide level in healthy humans.

  Sep. 2020, Nutrients, 12(9) (9), English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Daily consumption of black soybean (Glycine max L.) seed coat polyphenols attenuates dyslipidemia in apolipoprotein E-deficient mice

  Wataru Tanaka, Hiroki Matsuyama, Daigo Yokoyama, Yoko Yamashita, Hitoshi Ashida, Masanobu Sakono, Hiroyuki Sakakibara

  Elsevier BV, Sep. 2020, Journal of Functional Foods, 72, 104054 - 104054

  [Refereed]

  Scientific journal


• Black soybean seed coat polyphenols promote nitric oxide production in the aorta through the Akt/eNOS pathway

  Chiaki Domae, Hitoshi Ashida, Yoko Yamashita

  Background: Black soybean seed coat contains an abundance of flavan-3-ols and possesses various bioregulatory functions. Nitric oxide (NO) is produced by endothelial nitric oxide synthase (eNOS) in vascular endothelial cells and regulates vascular function through vasodilation and the inhibition of platelet aggregation in blood vessels. It has been reported that flavan-3-ols increase NO production, but many previous reports used a high concentration of flavan-3-ols. In the present study, we investigated the effect of flavan-3-ol-rich black soybean seed coat extract (BE) on NO production at a lower concentration that is close to the concentration after permeation through the monolayer of Caco-2 cells.Methods: Human umbilical vein endothelial cells (HUVEC) were incubated with BE, and then NO production in the medium and eNOS phosphorylation in the cells were examined. Intestinal epithelial Caco-2 cells on the upper side of a transwell filter were co-cultured with HUVEC on the basolateral compartment of the transwell apparatus. BE was added from the upper side, and the basolateral medium was collected to measure the concentration of NO and the content of flavan-3-ols. Furthermore, HUVEC were incubated with each flavan-3-ol in order to individuate the most effective compound in BE.Results: BE significantly increased NO production in the medium of HUVEC. When polyphenols in BE were removed from the basolateral medium by ethyl acetate extraction, increased NO production from HUVEC was not observed. Additionally, BE increased phosphorylation of eNOS and Akt in HUVEC. A portion of flavan-3-ols in BE had permeated through intestinal epithelial cells. Among the flavan-3-ols that had permeated, procyanidin C1 had the strongest effect on NO production in HUVEC at the concentration that had permeated the monolayer of Caco-2 cells. Procyanidin C1 (0.05 µM) also induced phosphorylation of eNOS and Akt in HUVEC without affecting the cAMP level. Conclusion: A portion of flavan-3-ols in BE directly promoted NO production through the Akt/eNOS pathway in vascular endothelial cells. These findings suggest that flavan-3-ols in the black soybean seed coat may contribute to improve the vascular function.Keywords: Black soybean seed coat polyphenols; NO; eNOS; Akt; vascular endothelial cells

  Functional Food Center, Aug. 2020, Functional Foods in Health and Disease, 10(8) (8), 330 - 330

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Black soybean improves the vascular function through an increase in nitric oxide and a decrease in oxidative stress in healthy women.

  Yoko Yamashita, Liuqing Wang, Asuka Nakamura, Fumio Nanba, Shizuka Saito, Toshiya Toda, Junichi Nakagawa, Hitoshi Ashida

  Vascular dysfunction and injurious stimuli such as oxidative stress is closely related to the risk of cardiovascular diseases (CVD). Dietary polyphenols is reported to exert the beneficial effects on reducing the risk of CVD. Black soybean is rich in polyphenols, including isoflavones, anthocyanidins and flavan-3-ols, and its prevention effects on CVD risk were reported in the animal experiments. In this study, we investigated the effect of black soybean consumption on the vascular function and oxidative stress associating with the polyphenol concentrations in healthy women. Lowered vascular age was observed in 33 out of 44 volunteers who completed the 8-week trial. It was observed that improvement of the vascular stiffness, increasing in the urinary NO2 and NO3 level, and decreasing in the oxidative stress markers, 8-hydroxy-2'-deoxyguanosine, hexanoyl-lysine and myeloperoxidase. In addition, concentration of 12 polyphenols in black soybean increased in the plasma and urine. Increased concentration of polyphenols would be involved in the decreased oxidative stress. Thus, black soybean consumption improved the vascular function through an increase in nitric oxide and a decrease in oxidative stress accompanied by increasing the polyphenol concentrations in healthy women.

  Jul. 2020, Archives of biochemistry and biophysics, 688, 108408 - 108408, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Cacao polyphenols regulate the circadian clock gene expression and through glucagon-like peptide-1 secretion.

  Ken-Yu Hironao, Yuji Mitsuhashi, Shujiao Huang, Hideaki Oike, Hitoshi Ashida, Yoko Yamashita

  Energy metabolism and circadian rhythms are closely related together, i.e., the timing of nutrient intake affects metabolism under the regulation of circadian rhythms. Previously, we have reported that cacao liquor procyanidin (CLPr) promotes energy metabolism, resulting in preventing obesity and hyperglycemia. However, it is not unclear whether CLPr regulates clock gene expression. In this study, we investigated whether the administration timing of CLPr affected clock gene expression and found that CLPr regulated the circadian clock gene expression through the glucagon-like peptide-1 (GLP-1) signaling pathway. CLPr administration at Zeitgeber time 3 increased the expression level of Per family and Dbp in the liver. At the same administration timing, CLPr increased GLP-1 and insulin concentration in the plasma and phosphorylation of AMPK in the liver. It was noteworthy that an antagonist for GLP-1 receptor Exendin (9-39) canceled CLPr-increased expression of Per family and Dbp and phosphorylation of AMPK in the liver, in addition to insulin secretion. These results strongly suggest that CLPr-induced GLP-1 regulates the changes in clock gene expression in the liver through increased insulin. Thus, CLPr is a possible functional food material for prevention and/or amelioration of metabolic disorders through preventing circadian disruption through GLP-1 and AMPK pathways.

  Jul. 2020, Journal of clinical biochemistry and nutrition, 67(1) (1), 53 - 60, English, Domestic magazine

  [Refereed]

  Scientific journal


• The cacao procyanidin extract-caused anti-hyperglycemic effect was changed by the administration timings.

  Ken-Yu Hironao, Hitoshi Ashida, Yoko Yamashita

  Mammals have the biological clocks with approximately 24 h-rhythm. Energy metabolism including glucose metabolism is regulated by the biological clocks. Glucose metabolism is affected by not only meal volume and its energy but also meal timing. We have reported that cacao liquor procyanidin-rich extract (CLPr) ameliorated the postprandial hyperglycemia through AMP-activated protein kinase pathway. However, the effect of administration timing of CLPr on the postprandial hyperglycemia and its signaling pathway are still unclear. In the present study, we compared the effect of CLPr-administration at the rest-phase (light-period) and active-phase (dark-period) on glucose metabolism. Single oral administration of CLPr to ICR mice at the rest-phase, but not at the active-phase, promoted phosphorylation of AMP-activated protein kinase and its upstream liver kinase B1 and translocation of glucose transporter 4 to the plasma membrane in the skeletal muscle, resulting in reduced postprandial hyperglycemia. These results indicated that the intake of CLPr at the rest-phase more effectively suppressed postprandial hyperglycemia.

  Jul. 2020, Journal of clinical biochemistry and nutrition, 67(1) (1), 61 - 66, English, Domestic magazine

  [Refereed]

  Scientific journal


• Bisacurone suppresses hepatic lipid accumulation through inhibiting lipogenesis and promoting lipolysis.

  Hitoshi Ashida, Xiaokuo Tian, Tomoya Kitakaze, Yoko Yamashita

  Turmeric and its components have various health beneficial functions. However, little is known about function of bisacurone, which is one of the sesquiterpenes in turmeric, at the compound level. In this study, we investigated the preventive effect of bisacurone on hepatic lipid accumulation and its mechanism in HepG2 cells and ICR mice. In HepG2 cells, bisacurone significantly inhibited fatty acid-induced intracellular lipid accumulation in a dose-dependent manner. Bisacurone at 10 µM increased protein expression of peroxisome proliferator-activated receptor α and carnitine palmitoyltransferase-1A accompanied by phosphorylation of AMP-activated protein kinase. In the liver of ICR mice, bisacurone decreased total lipids, triglyceride, and cholesterol contents. Bisacurone at 10 mg/kg body weight increased phosphorylation of AMP-activated protein kinase, and its downstream acetyl-CoA carboxylase as a rate-limiting enzyme for lipogenesis, while it decreased the nuclear translocation level of sterol regulatory element-binding protein 1 and carbohydrate-responsive element-binding protein as the major transcription factors for lipogenesis. On the other hand, bisacurone promoted lipolysis by up-expression of peroxisome proliferator-activated receptor α and carnitine palmitoyltransferase-1A. Thus, bisacurone might be a valuable food factor for preventing hepatic lipid accumulation by inhibiting lipogenesis and promoting lipolysis through phosphorylation of AMP-activated protein kinase.

  Jul. 2020, Journal of clinical biochemistry and nutrition, 67(1) (1), 43 - 52, English, Domestic magazine

  [Refereed]

  Scientific journal


• Prevention effect of quercetin and its glycosides on obesity and hyperglycemia through activating AMPKα in high-fat diet-fed ICR mice.

  Hao Jiang, Yuko Horiuchi, Ken-Yu Hironao, Tomoya Kitakaze, Yoko Yamashita, Hitoshi Ashida

  Quercetin and its glycosides possess various health beneficial functions, but comparative study of them on energy metabolism in different tissues are not well studied. In this study, we investigated AMP-activated protein kinase regulated glucose metabolism in the skeletal muscle and lipid metabolism in the white adipose tissue and liver to compare the effectiveness of quercetin and its glycosides, namely isoquercitrin, rutin, and enzymatically modified isoquercitrin, in male ICR mice. The mice were fed a standard or high-fat diet supplemented with 0.1% quercetin and its glycosides for 13 weeks. Quercetin glycosides, but not quercetin, decreased body weight gain and fat accumulation in the mesenteric adipose tissue in high-fat groups. All compounds decreased high-fat diet-increased plasma glucose and insulin levels. Moreover, all compounds significantly increased AMP-activated protein kinase phosphorylation in either standard or high-fat diet-fed mice in all tissues tested. As its downstream events, all compounds induced glucose transporter 4 translocation in the muscle. In the white adipose tissue and liver, all compounds increased lipogenesis while decreased lipolysis. Moreover, all compounds increased browning markers and decreased differentiation markers in adipose tissue. Therefore, quercetin and its glycosides are promising food components for prevention of adiposity and hyperglycemia through modulating AMP-activated protein kinase-driven pathways.

  Jul. 2020, Journal of clinical biochemistry and nutrition, 67(1) (1), 74 - 83, English, Domestic magazine

  [Refereed]

  Scientific journal


• 4-Hydroxyderricin and xanthoangelol isolated from Angelica keiskei prevent dexamethasone-induced muscle loss.

  Yasukiyo Yoshioka, Yumi Samukawa, Yoko Yamashita, Hitoshi Ashida

  Since a decrease in muscle mass leads to an increased risk of mortality, the prevention of muscle wasting contributes to maintaining the quality of life. Recently, we reported that glabridin, a prenylated flavonoid in licorice, prevents dexamethasone-induced muscle loss. In this study, we focused on the other prenylated chalcones 4-hydroxyderricin and xanthoangelol in Ashitaba (Angelica keiskei) and investigated their prevention effect on dexamethasone-induced muscle loss. It was found that 4-hydroxyderricin and xanthoangelol significantly prevented dexamethasone-induced protein degradation in C2C12 myotubes by suppressing the expression of ubiquitin ligases, Cbl-b and MuRF-1. These prenylated chalcones acted as the antagonists of the glucocorticoid receptor and inhibited the binding of dexamethasone to this receptor and its subsequent nuclear translocation. In addition, the chalcones suppressed the phosphorylation of p38 and FoxO3a as the upstream factors for ubiquitin ligases. Dexamethasone-induced protein degradation and upregulation of Cbl-b were attenuated by the knockdown of the glucocorticoid receptor but not by the knockdown of p38. In male C57BL/6J mice, the Ashitaba extract, containing 4-hydroxyderricin and xanthoangelol, suppressed dexamethasone-induced muscle mass wasting accompanied by a decrease in the expression of ubiquitin ligases by inhibiting the nuclear translocation of the glucocorticoid receptor and phosphorylation of FoxO3a. In conclusion, 4-hydroxyderricin and xanthoangelol are effective compounds to inhibit steroid-induced muscle loss.

  Jun. 2020, Food & function, 11(6) (6), 5498 - 5512, English, International magazine

  [Refereed]

  Scientific journal


• 6-(Methylsulfinyl)hexyl isothiocyanate protects acetaldehyde-caused cytotoxicity through the induction of aldehyde dehydrogenase in hepatocytes.

  Tomoya Kitakaze, Sihao Yuan, Masako Inoue, Yasukiyo Yoshioka, Yoko Yamashita, Hitoshi Ashida

  In the body, alcohol dehydrogenase rapidly converts ethanol to its toxic metabolite, acetaldehyde, which is further metabolized to non-toxic acetic acid by aldehyde dehydrogenase (ALDH). 6-(methylsulfinyl)hexyl isothiocyanate (6-MSITC), a major bioactive compound in Wasabi (Wasabia japonica) has various physiological effects such as anti-oxidative, anti-inflammatory and anti-cancer effects. However, the effect of 6-MSITC on alcohol metabolism has not been studied. In this study, we investigated the effects of 6-MSITC on hepatic ALDH activity and protein expression both in vitro and in vivo. 6-MSITC inhibited ethanol- and acetaldehyde-induced cytotoxicity. Treatment with 6-MSITC to HepG2 cells enhanced ALDH activity through the induction of mitochondrial ALDH2 expression, but not cytosolic ALDH1A1. Knockdown of Nrf2 canceled the 6-MSITC-induced ALDH2 expression, indicating that Nrf2 regulated ALDH2 expression. Moreover, 6-MSITC increased the nuclear translocation of Nrf2 and the expression levels of HO-1 and SOD2, Nrf2-regulated phase II drug-metabolizing enzymes. Oral administration of 6-MSITC increased the mitochondrial ALDH2 activity and its expression in the liver of C57BL/6J mice. These results suggested that 6-MSITC is possible to protect acetaldehyde toxicity in hepatocytes by induction of mitochondrial ALDH2 expression through Nrf2/ARE pathway.

  Jun. 2020, Archives of biochemistry and biophysics, 686, 108329 - 108329, English, International magazine

  [Refereed]

  Scientific journal


• Extracellular transglutaminase 2 induces myotube hypertrophy through G protein-coupled receptor 56.

  Tomoya Kitakaze, Miki Yoshikawa, Yasuyuki Kobayashi, Naohiro Kimura, Naoki Goshima, Takahiro Ishikawa, Yoshiyuki Ogata, Yoko Yamashita, Hitoshi Ashida, Naoki Harada, Ryoichi Yamaji

  Skeletal muscle secretes biologically active proteins that contribute to muscle hypertrophy in response to either exercise or dietary intake. The identification of skeletal muscle-secreted proteins that induces hypertrophy can provide critical information regarding skeletal muscle health. Dietary provitamin A, β-carotene, induces hypertrophy of the soleus muscle in mice. Here, we hypothesized that skeletal muscle produces hypertrophy-inducible secretory proteins via dietary β-carotene. Knockdown of retinoic acid receptor (RAR) γ inhibited the β-carotene-induced increase soleus muscle mass in mice. Using RNA sequencing, bioinformatic analyses, and literature searching, we predicted transglutaminase 2 (TG2) to be an all-trans retinoic acid (ATRA)-induced secretory protein in cultured C2C12 myotubes. Tg2 mRNA expression increased in ATRA- or β-carotene-stimulated myotubes and in the soleus muscle of β-carotene-treated mice. Knockdown of RARγ inhibited β-carotene-increased mRNA expression of Tg2 in the soleus muscle. ATRA increased endogenous TG2 levels in conditioned medium from myotubes. Extracellular TG2 promoted the phosphorylation of Akt, mechanistic target of rapamycin (mTOR), and ribosomal p70 S6 kinase (p70S6K), and inhibitors of mTOR, phosphatidylinositol 3-kinase, and Src (rapamycin, LY294002, and Src I1, respectively) inhibited TG2-increased phosphorylation of mTOR and p70S6K. Furthermore, extracellular TG2 promoted protein synthesis and hypertrophy in myotubes. TG2 mutant lacking transglutaminase activity exerted the same effects as wild-type TG2. Knockdown of G protein-coupled receptor 56 (GPR56) inhibited the effects of TG2 on mTOR signaling, protein synthesis, and hypertrophy. These results indicated that TG2 expression was upregulated through ATRA-mediated RARγ and that extracellular TG2 induced myotube hypertrophy by activating mTOR signaling-mediated protein synthesis through GPR56, independent of transglutaminase activity.

  Feb. 2020, Biochimica et biophysica acta. Molecular cell research, 1867(2) (2), 118563 - 118563, English, International magazine

  [Refereed]

  Scientific journal


• Low dose of luteolin activates Nrf2 in the liver of mice at start of the active phase but not that of the inactive phase.

  Tomoya Kitakaze, Atsushi Makiyama, Yoko Yamashita, Hitoshi Ashida

  A flavone luteolin has various health-promoting activities. Several studies reported that high dose of luteolin activates the Nrf2/ARE pathway in the liver. However, the effect of the low dose of luteolin that can be taken from a dietary meal on the Nrf2 activation remain unclear. It is expected that the flavonoid metabolism possesses a circadian rhythm, since nutritional metabolism processes daily cycle. In this study we investigated whether an administration affects the Nrf2 activation. ICR mice were orally administered 0.01-10 mg/kg body weight of luteolin once a day for 7 days at two time-points: at the start of active phase (ZT12) or at that of inactive phase (ZT0). Luteolin increased the nuclear translocation of Nrf2, resulting in the increases in its target gene products HO-1 and NQO1 at ZT12 but not at ZT0. The expression level of Nrf2 was lower at ZT12 than at ZT0 in the liver. We also found that the level of luteolin aglycon in the plasma is higher at ZT12 than at ZT0. These results suggest that the low dose of luteolin can activate Nrf2 pathway and the aglycon form of luteolin may mainly contribute to activate the Nrf2 pathway at ZT12 in the liver.

  2020, PloS one, 15(4) (4), e0231403, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Black soybean seed coat polyphenols promote nitric oxide production in the aorta through glucagon-like peptide-1 secretion from the intestinal cells.

  Chiaki Domae, Fumio Nanba, Toshinari Maruo, Toshio Suzuki, Hitoshi Ashida, Yoko Yamashita

  Black soybean seed coat polyphenols were reported to possess various bioregulatory functions. However, the effects of black soybean seed coat polyphenols on vascular functions are unknown. Vascular dysfunction caused by aging and vascular stiffness is associated with a risk of cardiovascular disease (CVD), and a reduction in nitric oxide (NO) levels can trigger the onset of CVD. In the present study, we investigated the effect of polyphenol-rich black soybean seed coat extract (BE) on vascular functions and the underlying mechanisms involved. The oral administration of BE at 50 mg per kg body weight to Wistar rats increased NO levels as determined by eNOS phosphorylation. The administration of BE also increased GLP-1 and cAMP levels. Furthermore, the effects of BE were inhibited in the presence of a GLP-1 receptor antagonist. This suggests that GLP-1 is strongly involved in the underlying mechanism of NO production in vivo. In conclusion, BE contributes to the improvement of vascular functions by promoting NO production. Regarding the putative underlying mechanism, GLP-1 secreted from intestinal cells by the polyphenols in BE activates eNOS in vascular endothelial cells.

  Dec. 2019, Food & function, 10(12) (12), 7875 - 7882, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Characterization of an organic-solvent-stable elastase from Pseudomonas indica and its potential use in eggshell membrane hydrolysis

  Takenaka Shinji, Yokoyama Moe, Kimura Yukihiro, Yamashita Yoko, Ashida Hitoshi

  Oct. 2019, PROCESS BIOCHEMISTRY, 85, 156 - 163

  [Refereed]

  Scientific journal


• Enzymatically modified isoquercitrin promotes energy metabolism through activating AMPKα in male C57BL/6 mice.

  Hao Jiang, Yasukiyo Yoshioka, Sihao Yuan, Yuko Horiuchi, Yoko Yamashita, Kevin D Croft, Hitoshi Ashida

  Quercetin possesses various health beneficial functions, but its poor bioavailability limits these functions. Enzymatically modified isoquercitrin (EMIQ) is a quercetin glycoside with a greater bioavailability than quercetin. In this study, we investigated whether EMIQ regulates energy metabolism in mice and its underlying molecular mechanism. Male C57BL/6 mice were fed a normal diet with different doses of EMIQ or quercetin (0.02%, 0.1% and 0.5%) for two weeks. Supplementation with 0.1% EMIQ significantly decreased white adipose tissue (WAT) weight. Supplementation with 0.02% and 0.1% EMIQ promoted phosphorylation of adenosine monophosphate activated protein kinase (AMPK) in the WAT, liver, and muscle. In the WAT, 0.1% EMIQ downregulated peroxisome proliferator-activated receptor (PPAR)γ, CCAAT-enhancer-binding protein (C/EBP)α, C/EBPβ, and sterol regulatory element-binding protein 1 expression, as well as upregulated mitochondrial uncoupling protein (UCP) 2 and carnitine palmitoyltransferase-1 expression. Supplementation with 0.1% EMIQ also promoted the expression of thermogenesis-associated factors including PPARγ coactivator α (PGC-1α), UCP1, PR-domain containing protein 16, and sirtuin 1 in the WAT. In the liver, EMIQ promoted the phosphorylation of acetyl-CoA carboxylase, and increased the expression of PPARα, constitutive androstane-receptor, and farnesoid X receptor. Furthermore, supplementation with 0.02% or 0.1% EMIQ suppressed the plasma glucose level accompanied by the translocation of glucose transporter 4 to the plasma membrane of the muscle. Our results suggest that EMIQ is a potential food additive for the regulation of energy metabolism through AMPK phosphorylation.

  Aug. 2019, Food & function, 10(8) (8), 5188 - 5202, English, International magazine

  [Refereed]

  Scientific journal


• Green Tea Ameliorates Hyperglycemia by Promoting the Translocation of Glucose Transporter 4 in the Skeletal Muscle of Diabetic Rodents.

  Manabu Ueda-Wakagi, Hironobu Nagayasu, Yoko Yamashita, And Hitoshi Ashida

  It is known that green tea helps prevent obesity and diabetes mellitus. In this study, we aimed to determine whether green tea ameliorates hyperglycemia and the mechanism involved in diabetic rodents. Green tea consumption reduced blood glucose and ameliorated glucose intolerance, which was assessed using an oral glucose tolerance test in both streptozotocin-induced type 1 diabetic rats and type 2 diabetic KK-Ay mice. Green tea also reduced the plasma fructosamine and glycated hemoglobin concentrations in both models. Furthermore, it increased glucose uptake into the skeletal muscle of both model animals, which was accompanied by greater translocation of glucose transporter 4 (GLUT4). Moreover, epigallocatechin gallate (EGCG), the principal catechin in green tea, also ameliorated glucose intolerance in high-fat diet-induced obese and diabetic mice. These results suggest that green tea can ameliorate hyperglycemia in diabetic rodents by stimulating GLUT4-mediated glucose uptake in skeletal muscle, and that EGCG is one of the effective compounds that mediate this effect.

  May 2019, International journal of molecular sciences, 20(10) (10), English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Liquorice flavonoid oil suppresses hyperglycaemia accompanied by skeletal muscle myocellular GLUT4 recruitment to the plasma membrane in KK-Ay mice.

  Yoko Yamashita, Hideyuki Kishida, Kaku Nakagawa, Yasukiyo Yoshioka, Hitoshi Ashida

  For over 4000 years, liquorice has been one of the most frequently employed botanicals as a traditional herbal medicine. Although previous reports have found that liquorice flavonoids possess various health beneficial effects, the underlying mechanism responsible for the anti-diabetic effect of liquorice flavonoids remains unclear. The present study demonstrates that liquorice flavonoid oil (LFO) improves type 2 diabetes mellitus through GLUT4 translocation to the plasma membrane by activating both the adenosine monophosphate-activated protein kinase (AMPK) pathway and Akt pathway in muscle of KK-Ay mice. Furthermore, LFO lowered postprandial hyperglycaemia in a human study. These results indicate that LFO may exert a therapeutic effect on metabolic disorders, such as diabetes and hyperglycaemia, by modulating glucose metabolism through AMPK- and insulin-dependent pathways in skeletal muscle.

  May 2019, International journal of food sciences and nutrition, 70(3) (3), 294 - 302, English, International magazine

  [Refereed]

  Scientific journal


• Glabridin inhibits dexamethasone-induced muscle atrophy.

  Yasukiyo Yoshioka, Yusuke Kubota, Yumi Samukawa, Yoko Yamashita, Hitoshi Ashida

  Prevention of muscle wasting is known to contribute to improving the quality of life and extending a healthy life. Recently, we have reported that licorice flavonoid oil containing glabridin, which is a prenylated isoflavone, enhances muscle mass in mice. In this study, we investigated the prevention effect of glabridin on dexamethasone-induced muscle atrophy and clarified its mechanism in cultured myotubes and in muscle of mice. Treatment with glabridin to C2C12 myotubes inhibited dexamethasone-induced protein degradation through dexamethasone-induced expression of ubiquitin ligases, MuRF1 and Cbl-b, but not atrogin-1. Mechanistically, glabridin inhibited nuclear translocation of the glucocorticoid receptor. Glabridin directly bound to the glucocorticoid receptor, resulting in the inhibition of binding between dexamethasone and the receptor protein. Glabridin also inhibited dexamethasone-induced phosphorylation of p38 and FoxO3a, as the upstream for the induction of ubiquitin ligases in C2C12 myotubes. Moreover, the glabridin-induced inhibition of protein degradation was eliminated by knockdown of the glucocorticoid receptor, but not by p38 knockdown. These data indicated that the inhibitory mechanism of glabridin against dexamethasone-induced muscle atrophy was mainly mediated by the inhibition of binding between dexamethasone and the glucocorticoid receptor in myotubes. Oral administration of glabridin prevented dexamethasone-induced protein degradation in the tibialis anterior muscle of mice. It was confirmed that glabridin inhibited dexamethasone-induced nuclear translocation of the glucocorticoid receptor and phosphorylation of FoxO3a in the muscle of mice. These findings suggest that glabridin is an effective food ingredient for the prevention of glucocorticoid-induced skeletal muscle atrophy.

  Mar. 2019, Archives of biochemistry and biophysics, 664, 157 - 166, English, International magazine

  [Refereed]

  Scientific journal


• A physiological concentration of luteolin induces phase II drug-metabolizing enzymes through the ERK1/2 signaling pathway in HepG2 cells.

  Tomoya Kitakaze, Atsushi Makiyama, Yumi Samukawa, Songyan Jiang, Yoko Yamashita, Hitoshi Ashida

  The flavon luteolin has various health-promoting activities including cardiovascular protection, anti-inflammatory activity and anticancer activity. A serum concentration of about 100 nM luteolin is reached by dietary habit. However, little is known about the function of luteolin over its physiological concentration range. In this study, we investigated whether a physiological concentration of luteolin could activate nuclear factor-erythroid-2-related factor 2 (Nrf2)-mediated expression of phase II drug-metabolizing enzymes in human hepatoma HepG2 cells. Interestingly, less than 1 nM of luteolin could induce phase II drug-metabolizing enzymes, such as GSTs, HO-1, and NQO1. Both 1 and 100 nM luteolin increased expression and activity of ALDH2, which metabolized toxic acetaldehyde into nontoxic acetic acid. Luteolin increased nuclear accumulation of Nrf2 and enhanced the ARE-binding complex through increasing the stability of the Nrf2 protein. Luteolin increased phosphorylation of Nrf2 at Ser40, and MEK inhibitors (U0126 and PD98059) canceled luteolin-induced phosphorylation of Nrf2. Furthermore, luteolin increased modified Keap1. In conclusion, a physiological concentration of luteolin induces the expression of phase II drug-metabolizing enzymes by enhancement of Nrf2 nuclear accumulation through MEK1/2-ERK1/2-mediated phosphorylation of Nrf2, increasing Nrf2 stability and inducing a conformational change of Keap1.

  Mar. 2019, Archives of biochemistry and biophysics, 663, 151 - 159, English, International magazine

  [Refereed]

  Scientific journal


• Quercetin and its metabolite isorhamnetin promote glucose uptake through different signalling pathways in myotubes.

  Hao Jiang, Yoko Yamashita, Asuka Nakamura, Kevin Croft, Hitoshi Ashida

  Quercetin and its metabolite isorhamnetin elicit various beneficial effects on human health. However, their bioavailability is low. In this study, we investigated whether low concentrations in the physiological range could promote glucose uptake in L6 myotubes, as well as the underlying molecular mechanisms. We found that 0.1 nM and 1 nM quercetin or 1 nM isorhamnetin significantly increased glucose uptake via translocation of glucose transporter type 4 (GLUT4) to the plasma membrane of L6 myotubes. Quercetin principally activated the CaMKKβ/AMPK signalling pathway at these concentrations, but also activated IRS1/PI3K/Akt signalling at 10 nM. In contrast, 1 nM and 10 nM isorhamnetin principally activated the JAK/STAT pathway. Treatment with siAMPKα and siJAK2 abolished quercetin- and isorhamnetin-induced GLUT4 translocation, respectively. However, treatment with siJAK3 did not affect isorhamnetin-induced GLUT4 translocation, indicating that isorhamnetin induced GLUT4 translocation mainly through JAK2, but not JAK3, signalling. Thus, quercetin preferably activated the AMPK pathway and, accordingly, stimulated IRS1/PI3K/Akt signalling, while isorhamnetin activated the JAK2/STAT pathway. Furthermore, after oral administration of quercetin glycoside at 10 and 100 mg/kg body weight significantly induced GLUT4 translocation to the plasma membrane of skeletal muscles in mice. In the same animals, plasma concentrations of quercetin aglycone form were 4.95 and 6.80 nM, respectively. In conclusion, at low-concentration ranges, quercetin and isorhamnetin promote glucose uptake by increasing GLUT4 translocation via different signalling pathways in skeletal muscle cells; thus, these compounds may possess beneficial functions for maintaining glucose homeostasis by preventing hyperglycaemia at physiological concentrations.

  Feb. 2019, Scientific reports, 9(1) (1), 2690 - 2690, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of Microbial Metabolites of (-)-Epigallocatechin Gallate on Glucose Uptake in L6 Skeletal Muscle Cell and Glucose Tolerance in ICR Mice.

  Akiko Takagaki, Yasukiyo Yoshioka, Yoko Yamashita, Tomoya Nagano, Masaki Ikeda, Aya Hara-Terawaki, Ryota Seto, Hitoshi Ashida

  Glucose uptake ability into L6 skeletal muscle cell was examined with eleven kinds of ring fission metabolites of (-)-epigallocatechin gallate (EGCG) produced by intestinal bacteria. The metabolites 5-(3,5-dihydroxyphenyl)-γ-valerolactone (EGC-M5), 4-hydroxy-5-(3,4,5-trihydroxyphenyl)valeric acid (EGC-M6), 5-(3,4,5-trihydroxyphenyl)-γ-valerolactone (EGC-M7) and 5-(3-hydroxyphenyl)valeric acid (EGC-M11) have been found to promote uptake of glucose into L6 myotubes significantly. EGC-M5, which is one of the major ring fission metabolites of EGCG, was also found to have a promotive effect on glucose transporter 4 (GLUT4) translocation accompanied by phosphorylation of AMP-activated protein kinase (AMPK) signaling pathway in skeletal muscle both in vivo and in vitro. Furthermore, the effect of oral single dosage of EGC-M5 on glucose tolerance test with ICR mice was examined and significant suppression of hyperglycemia was observed. These data suggested that EGC-M5 has an antidiabetic effect in vivo.

  2019, Biological & pharmaceutical bulletin, 42(2) (2), 212 - 221, English, Domestic magazine

  [Refereed]

  Scientific journal


• Cacao liquor procyanidins prevent postprandial hyperglycaemia by increasing glucagon-like peptide-1 activity and AMP-activated protein kinase in mice.

  Yoko Yamashita, Masaaki Okabe, Midori Natsume, Hitoshi Ashida

  Procyanidins have been reported to possess potential for the prevention of hyperglycaemia. However, there are very few data for procyanidins about the difference the degree of polymerisation (DP) has on anti-hyperglycaemic effects. Moreover, the underlying molecular mechanisms by which procyanidins suppress hyperglycaemia are not yet fully understood. In the present study, we prepared procyanidin fractions with different DP, namely low-DP (DP≤3) and high-DP (DP≥4) fractions, from a cacao liquor procyanidin-rich extract (CLPr). These fractions were administered orally to Institute of Cancer Research (ICR) mice and their anti-hyperglycaemic effects were examined. We found that CLPr and its fractions prevent postprandial hyperglycaemia accompanied by an increase in the plasma glucagon-like peptide-1 (GLP-1) level with or without glucose load. In the absence of glucose load, both fractions increased the plasma insulin level and activated its downstream signalling pathway in skeletal muscle, resulting in promotion of the translocation of GLUT4. Phosphorylation of AMP-activated protein kinase (AMPK) was also involved in the promotion of GLUT4 translocation. High- and low-DP fractions showed a similar activation of insulin and AMPK pathways. In conclusion, cacao liquor procyanidins prevent hyperglycaemia by promoting GLUT4 translocation in skeletal muscle, and both the GLP-1-activated insulin pathway and the AMPK pathway are involved in the underlying molecular mechanism.

  2019, Journal of nutritional science, 8, e2, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Absorption, metabolism, distribution and faecal excretion of B-type procyanidin oligomers in mice after a single oral administration of black soybean seed coat extract.

  Liuqing Wang, Yoko Yamashita, Shingo Komeda, Akiko Saito, Hitoshi Ashida

  We investigated the absorption, metabolism, distribution and faecal excretion of 3 B-type procyanidin oligomers, including procyanidin B2, procyanidin C1 and cinnamtannin A2, and their monomeric unit (-)-epicatechin after a single oral administration of black soybean seed coat extract (BE) to male ICR mice at 250 mg per kg body weight. Plasma, tissues and faeces samples were collected within 24 h for the determination of (-)-epicatechin, procyandidin B2, procyanidin C1 and cinnamtannin A2 with or without β-glucuronidase and sulfatase treatment by the high-performance liquid chromatography method. A portion of the B-type procyanidin oligomers and (-)-epicatechin in BE was absorbed from the small intestine after the oral administration of BE. In the plasma, absorbed procyanidins and (-)-epicatechin existed mainly as conjugates. In the tissues, procyanidin B2, procyandin C1 and cinnamtannin A2, in addition to (-)-epicatechin distributed widely, primarily in their free forms. Their conjugation occurred mainly in the small intestine, rather than in the liver. Monomeric unit (-)-epicatechin had the highest bioavailability, followed by procyanidin B2, procyanidin C1 and cinnamtannin A2.

  Oct. 2018, Food & function, 9(10) (10), 5362 - 5370, English, International magazine

  [Refereed]

  Scientific journal


• Licorice flavonoid oil enhances muscle mass in KK-Ay mice.

  Yasukiyo Yoshioka, Yoko Yamashita, Hideyuki Kishida, Kaku Nakagawa, Hitoshi Ashida

  Elsevier Inc., Jul. 2018, Life sciences, 205, 91 - 96, English, International magazine

  [Refereed]

  Scientific journal


• Piperine Promotes Glucose Uptake through ROS-Dependent Activation of the CAMKK/AMPK Signaling Pathway in Skeletal Muscle.

  Ayumi Maeda, Takeshi Shirao, Daishi Shirasaya, Yasukiyo Yoshioka, Yoko Yamashita, Mitsugu Akagawa, Hitoshi Ashida

  SCOPE: The prevalence of type 2 diabetes mellitus (T2DM) is increasing yearly worldwide. Glycemic control is the basis for the treatment of T2DM, as it can prevent the progress of associated complications. Spices possess various health beneficial effects on humans. The aim of this study is to search for spices that can promote glucose uptake and to elucidate the underlying molecular mechanism(s). METHODS AND RESULTS: Among 24 spice extracts, the extracts from black pepper and white pepper significantly increase glucose uptake in L6 myotubes. Piperine is found to be the active compound in these extracts. Treatment of myotubes with piperine induces the translocation of glucose transporter 4 (GLUT4) to the plasma membrane by phosphorylation of AMP-activated protein kinase (AMPK). Piperine increases the intracellular Ca2+ level and reactive oxygen species (ROS) generation through transient receptor potential vanilloid channel 1 (TRPV1), followed by activation of Ca2+ /calmodulin-dependent protein kinase kinase-beta (CaMKKβ) as the upstream events for AMPK phosphorylation. Furthermore, oral administration of piperine to Wistar rats at 0.01 and 0.1 mg kg-1 body weight decreases postprandial hyperglycemia accompanied by GLUT4 translocation and AMPK phosphorylation. CONCLUSION: Piperine in pepper prevents hyperglycemia by GLUT4 translocation through CaMKKβ/AMPK signaling via TRPV1-dependent increase in the intracellular Ca2+ level and ROS generation.

  Jun. 2018, Molecular nutrition & food research, 62(11) (11), e1800086, English, International magazine

  [Refereed]

  Scientific journal


• Screening plant derived dietary phenolic compounds for bioactivity related to cardiovascular disease.

  Kevin D Croft, Yoko Yamashita, Helen O'Donoghue, Daishi Shirasaya, Natalie C Ward, Hitoshi Ashida

  The potential health benefits of phenolic acids found in food and beverages has been suggested from a number of large population studies. However, the mechanism of how these compounds may exert biological effects is less well established. It is also now recognised that many complex polyphenols in the diet are metabolised to simple phenolic acids which can be taken up in the circulation. In this paper a number of selected phenolic compounds have been tested for their bioactivity in two cell culture models. The expression and activity of endothelial nitric oxide synthase (eNOS) in human aortic endothelial cells and the uptake of glucose in muscle cells. Our data indicate that while none of the compounds tested had a significant effect on eNOS expression or activation in endothelial cells, several of the compounds increased glucose uptake in muscle cells. These compounds also enhanced the translocation of the glucose transporter GLUT4 to the plasma membrane, which may explain the observed increase in cellular glucose uptake. These results indicate that simple cell culture models may be useful to help understand the bioactivity of phenolic compounds in relation to cardiovascular protection.

  Apr. 2018, Fitoterapia, 126, 22 - 28, English, International magazine

  [Refereed]

  Scientific journal


• Curcumin and its derivatives inhibit 2,3,7,8,-tetrachloro-dibenzo-p-dioxin-induced expression of drug metabolizing enzymes through aryl hydrocarbon receptor-mediated pathway.

  Rika Nakai, Shun Fukuda, Masaya Kawase, Yoko Yamashita, Hitoshi Ashida

  Japan Society for Bioscience Biotechnology and Agrochemistry, Apr. 2018, Bioscience, biotechnology, and biochemistry, 82(4) (4), 616 - 628, English, International magazine

  [Refereed]

  Scientific journal


• Effects of black soybean seed coat extract on improving endothelial vascular function - A randomized, double-blind, placebo-controlled, crossover study

  Fumio Nanba, Toshiya Toda, Masahiro Fukuda, Yoko Yamashita, Hitoshi Ashida

  Life Science Publishing Co. Ltd, 2018, Japanese Pharmacology and Therapeutics, 46(5) (5), 857 - 868, Japanese

  Scientific journal


• Methylxanthine Derivative-Rich Cacao Extract Suppresses Differentiation of Adipocytes through Downregulation of PPARγ and C/EBPs.

  Yoko Yamashita, Takakazu Mitani, Liuqing Wang, Hitoshi Ashida

  Center for Academic Publications Japan, 2018, Journal of nutritional science and vitaminology, 64(2) (2), 151 - 160, English, Domestic magazine

  [Refereed]

  Scientific journal


• Flavan 3-ol delays the progression of disuse atrophy induced by hindlimb suspension in mice.

  Mao Ito, Naoto Kudo, Yuji Miyake, Tatsuya Imai, Tomoki Unno, Yoko Yamashita, Yoshihisa Hirota, Hitoshi Ashida, Naomi Osakabe

  Periods of skeletal muscle disuse, for example due to a sedentary lifestyle or bed rest, are associated with aging and can lead to muscle atrophy. We previously found that the flavan 3-ol fraction derived from cocoa (FL) enhanced energy expenditure with metabolic changes in skeletal muscle. In the present study, we examined the effect of FL on disuse muscle atrophy induced by hindlimb suspension in mice. Male C57BL/6J mice were assigned to four groups as follows: unsuspended-vehicle, unsuspended-FL, suspended-vehicle, and suspended-FL. Mice in the vehicle treatment groups were administered distilled water and those in the FL treatment groups were dosed with FL (50mg/kg/day) for 2weeks. The weights of the gastrocnemius (GC), tibialis anterior (TA), and soleus (SOL), but not the extensor digitorum longus (EDL), decreased significantly in mice with hindlimb suspension (-11.8%, -16.5%, and -41.0%, respectively). This reduction in GC, TA, and SOL mass was inhibited by FL (-5.3%, +2.0%, and -16.6%, respectively). The FL increased the EDL weight >20% with or without hindlimb suspension. The protein level of the ubiquitin ligase, muscle ring finger-1, in the SOL was significantly increased by hindlimb suspension, but inhibited by treatment with FL. Protein expression of p70S6 kinase in the SOL was significantly decreased by hindlimb suspension, and FL treatment inhibited this change. These results suggested that FL delayed disuse muscle atrophy by metabolic alteration.

  Nov. 2017, Experimental gerontology, 98, 120 - 123, English, International magazine

  [Refereed]

  Scientific journal


• The interaction of auraptene and other oxyprenylated phenylpropanoids with glucose transporter type 4.

  Salvatore Genovese, Hitoshi Ashida, Yoko Yamashita, Tomoya Nakgano, Masaki Ikeda, Shirasaya Daishi, Francesco Epifano, Vito Alessandro Taddeo, Serena Fiorito

  Aug. 2017, Phytomedicine : international journal of phytotherapy and phytopharmacology, 32, 74 - 79, English, International magazine

  [Refereed]

  Scientific journal


• An analysis method for flavan-3-ols using high performance liquid chromatography coupled with a fluorescence detector.

  Liuqing Wang, Yoko Yamashita, Akiko Saito, Hitoshi Ashida

  Jul. 2017, Journal of food and drug analysis, 25(3) (3), 478 - 487, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Enzymatically synthesized glycogen inhibits colitis through decreasing oxidative stress.

  Takakazu Mitani, Yasukiyo Yoshioka, Takashi Furuyashiki, Yoko Yamashita, Yasuhito Shirai, Hitoshi Ashida

  May 2017, Free radical biology & medicine, 106, 355 - 367, English, International magazine

  [Refereed]

  Scientific journal


• Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells.

  Yasukiyo Yoshioka, Xiu Li, Tianshun Zhang, Takakazu Mitani, Michiko Yasuda, Fumio Nanba, Toshiya Toda, Yoko Yamashita, Hitoshi Ashida

  Mar. 2017, Journal of clinical biochemistry and nutrition, 60(2) (2), 108 - 114, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Caffeine-Stimulated Intestinal Epithelial Cells Suppress Lipid Accumulation in Adipocytes.

  Takakazu Mitani, Tomoya Nagano, Kiyonari Harada, Yoko Yamashita, Hitoshi Ashida

  Center for Academic Publications Japan, 2017, Journal of nutritional science and vitaminology, 63(5) (5), 331 - 338, English, Domestic magazine

  [Refereed]

  Scientific journal


• Rapid Preparation of a Plasma Membrane Fraction: Western Blot Detection of Translocated Glucose Transporter 4 from Plasma Membrane of Muscle and Adipose Cells and Tissues.

  Norio Yamamoto, Yoko Yamashita, Yasukiyo Yoshioka, Shin Nishiumi, Hitoshi Ashida

  Membrane proteins account for 70% to 80% of all pharmaceutical targets, indicating their clinical relevance and underscoring the importance of identifying differentially expressed membrane proteins that reflect distinct disease properties. The translocation of proteins from the bulk of the cytosol to the plasma membrane is a critical step in the transfer of information from membrane-embedded receptors or transporters to the cell interior. To understand how membrane proteins work, it is important to separate the membrane fraction of cells. This unit provides a protocol for rapidly obtaining plasma membrane fractions for western blot analysis. © 2016 by John Wiley & Sons, Inc.

  Aug. 2016, Current protocols in protein science, 85, 29.18.1-29.18.12 - 29.18.12, English, International magazine

  [Refereed]

  Scientific journal


• Procyanidin Promotes Translocation of Glucose Transporter 4 in Muscle of Mice through Activation of Insulin and AMPK Signaling Pathways.

  Yoko Yamashita, Liuqing Wang, Fumio Nanba, Chiaki Ito, Toshiya Toda, Hitoshi Ashida

  2016, PloS one, 11(9) (9), e0161704, English, International magazine

  [Refereed]

  Scientific journal


• beta-Conglycinin Peptides Improve Glucose Uptake through the AMPK Signaling Pathway in L6 Myotubes

  Yoko Yamashita, Manabu Ueda-Wakagi, Mai Sakamoto, Nobuhiko Tachibana, Satoshi Wanezaki, Mitsutaka Kohno, Hitoshi Ashida

  Sep. 2015, FOOD SCIENCE AND TECHNOLOGY RESEARCH, 21(5) (5), 727 - 732, English

  [Refereed]

  Scientific journal


• Black Tea Polyphenols Promotes GLUT4 Translocation through Both PI3K-and AMPK-dependent Pathways in Skeletal Muscle Cells

  Tomoya Nagano, Kaori Hayashibara, Manabu Ueda-Wakagi, Yoko Yamashita, Hitoshi Ashida

  May 2015, FOOD SCIENCE AND TECHNOLOGY RESEARCH, 21(3) (3), 489 - 494, English

  [Refereed]

  Scientific journal


• Luteolin suppresses TCDD-induced wasting syndrome in a cultured adipocyte model.

  Hitoshi Ashida, Kiyonari Harada, Sakiho Mishima, Takakazu Mitani, Yoko Yamashita, Fumio Matsumura

  May 2015, Pesticide biochemistry and physiology, 120, 14 - 20, English, International magazine

  [Refereed]

  Scientific journal


• Ashitaba (Angelica keiskei) extract prevents adiposity in high-fat diet-fed C57BL/6 mice.

  Tianshun Zhang, Yoko Yamashita, Michiko Yasuda, Norio Yamamoto, Hitoshi Ashida

  Jan. 2015, Food & function, 6(1) (1), 135 - 45, English, International magazine

  [Refereed]

  Scientific journal


• Oolong, black and pu-erh tea suppresses adiposity in mice via activation of AMP-activated protein kinase.

  Yoko Yamashita, Liuqing Wang, Lihua Wang, Yuki Tanaka, Tianshun Zhang, Hitoshi Ashida

  Oct. 2014, Food & function, 5(10) (10), 2420 - 9, English, International magazine

  [Refereed]

  Scientific journal


• Luteolin modulates expression of drug-metabolizing enzymes through the AhR and Nrf2 pathways in hepatic cells.

  Tianshun Zhang, Yuki Kimura, Songyan Jiang, Kiyonari Harada, Yoko Yamashita, Hitoshi Ashida

  Sep. 2014, Archives of biochemistry and biophysics, 557, 36 - 46, English, International magazine

  [Refereed]

  Scientific journal


• Glabridin induces glucose uptake via the AMP-activated protein kinase pathway in muscle cells.

  Keisuke Sawada, Yoko Yamashita, Tianshun Zhang, Kaku Nakagawa, Hitoshi Ashida

  Aug. 2014, Molecular and cellular endocrinology, 393(1-2) (1-2), 99 - 108, English, International magazine

  [Refereed]

  Scientific journal


• The chalcones cardamonin and flavokawain B inhibit the differentiation of preadipocytes to adipocytes by activating ERK.

  Tianshun Zhang, Norio Yamamoto, Yoko Yamashita, Hitoshi Ashida

  Jul. 2014, Archives of biochemistry and biophysics, 554, 44 - 54, English, International magazine

  [Refereed]

  Scientific journal


• Soy β-conglycinin improves glucose uptake in skeletal muscle and ameliorates hepatic insulin resistance in Goto-Kakizaki rats.

  Nobuhiko Tachibana, Yoko Yamashita, Mayuko Nagata, Satoshi Wanezaki, Hitoshi Ashida, Fumihiko Horio, Mitsutaka Kohno

  Feb. 2014, Nutrition research (New York, N.Y.), 34(2) (2), 160 - 7, English, International magazine

  [Refereed]

  Scientific journal


• Luteolin modulates expression of drug-metabolizing enzymes through AhR and Nrf2 pathways in hepatocytes

  KIMURA Yuki, ZHANG Tianshun, JIANG Songyan, YAMASHITA Yoko, ASHIDA Hitoshi

  2014, Polyphenols Communications 2014 (Proceedings for XXVIth International Conference on Polyphenols & 8th Tannin Conference), 393 - 394, English

  International conference proceedings


• Glabridin suppresses hyperglycemia through promotion of glucose uptake accompanied by translocation of glucose transporter 4 in muscle

  YAMASHITA Yoko, SAWADA Keisuke, KISHIDA Hideyuki, ZNAKAGAWA Kaku, ASHIDA Hitoshi

  2014, Polyphenols Communications 2014 (Proceedings for XXVIth International Conference on Polyphenols & 8th Tannin Conference), 361 - 362, English

  International conference proceedings


• Fermented tea suppresses adiposity through the activation of AMPK and modulation of its downstream events.

  YAMASHITA Yoko, WANG Liuqing, WANG Lihua, TANAKA Yuki, ZHANG Tianshun, ASHIDA Hitoshi

  2014, Food and Functions, 5, 2420 - 2429, English

  [Refereed]

  Scientific journal


• Effects of luteolin on TCDD- and tert-butylhydroquinone-induced drug-metabolizing enzynes and nuclear factor-erythroid-2-related factor 2.

  ASHIDA Hitoshi, ZHANG Tianshun, KIMURA Yuki, JIANG Songyan, HARADA Kiyonari, YAMASHITA Yoko

  2014, Organohalogen Compounds, 76, 317 - 320, English

  Scientific journal


• Bioavailability of procyanidins in mice

  WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

  2014, Polyphenols Communications 2014 (Proceedings for XXVIth International Conference on Polyphenols & 8th Tannin Conference), 589 - 590, English

  International conference proceedings


• 10-Hydroxy-2-decenoic acid, a unique medium-chain fatty acid, activates 5'-AMP-activated protein kinase in L6 myotubes and mice.

  Masathito Takikawa, Aya Kumagai, Harumi Hirata, Minoru Soga, Yoko Yamashita, Manabu Ueda, Hitoshi Ashida, Takanori Tsuda

  Oct. 2013, Molecular nutrition & food research, 57(10) (10), 1794 - 802, English, International magazine

  [Refereed]

  Scientific journal


• Black soybean seed coat extract ameliorates hyperglycemia and insulin sensitivity via the activation of AMP-activated protein kinase in diabetic mice.

  Yuta Kurimoto, Yuki Shibayama, Seiya Inoue, Minoru Soga, Masahito Takikawa, Chiaki Ito, Fumio Nanba, Tadashi Yoshida, Yoko Yamashita, Hitoshi Ashida, Takanori Tsuda

  Jun. 2013, Journal of agricultural and food chemistry, 61(23) (23), 5558 - 64, English, International magazine

  [Refereed]

  Scientific journal


• Detection of orally administered inositol stereoisomers in mouse blood plasma and their effects on translocation of glucose transporter 4 in skeletal muscle cells.

  Yoko Yamashita, Masaru Yamaoka, Tomohisa Hasunuma, Hitoshi Ashida, Ken-ichi Yoshida

  May 2013, Journal of agricultural and food chemistry, 61(20) (20), 4850 - 4, English, International magazine

  [Refereed]

  Scientific journal


• Black soybean seed coat polyphenols prevent B(a)P-induced DNA damage through modulating drug-metabolizing enzymes in HepG2 cells and ICR mice.

  Tianshun Zhang, Songyan Jiang, Chao He, Yuki Kimura, Yoko Yamashita, Hitoshi Ashida

  Apr. 2013, Mutation research, 752(1-2) (1-2), 34 - 41, English, International magazine

  [Refereed]

  Scientific journal


• 大豆たん白質由来ペプチドによる筋肉細胞への糖取込み活性促進作用とその作用機序の解明

  SAWADA keisuke, YAMASHITA Yoko, ASHIDA Hitoshi

  不二たん白質研究振興財団, 2013, 大豆たん白質研究, 15(33) (33), 72 - 78, Japanese

  Symposium


• Effect of green tea extract on copper dynamics in mouse hair

  Masaya Kawase, Tadashi Saito, Yukihiro Nakano, Yoko Yamashita, Sayuri Imada, Hitoshi Ashida

  2013, Food Science and Technology Research, 19(1) (1), 123 - 125, English

  [Refereed]

  Scientific journal


• Cinnamtannin A2, a tetrameric procyanidin, increases GLP-1 and insulin secretion in mice.

  Yoko Yamashita, Masaaki Okabe, Midori Natsume, Hitoshi Ashida

  2013, Bioscience, biotechnology, and biochemistry, 77(4) (4), 888 - 91, English, International magazine

  [Refereed]

  Scientific journal


• Prevention mechanisms of glucose intolerance and obesity by cacao liquor procyanidin extract in high-fat diet-fed C57BL/6 mice.

  Yoko Yamashita, Masaaki Okabe, Midori Natsume, Hitoshi Ashida

  Nov. 2012, Archives of biochemistry and biophysics, 527(2) (2), 95 - 104, English, International magazine

  [Refereed]

  Scientific journal


• Fermented tea improves glucose intolerance in mice by enhancing translocation of glucose transporter 4 in skeletal muscle.

  Yoko Yamashita, Lihua Wang, Zhang Tinshun, Toshiyuki Nakamura, Hitoshi Ashida

  Nov. 2012, Journal of agricultural and food chemistry, 60(45) (45), 11366 - 71, English, International magazine

  [Refereed]

  Scientific journal


• Comparison of Anti-Hyperglycemic Activities Between Low- and High-Degree of Polymerization Procyanidin Fractions from Cacao Liquor Extract

  Yoko Yamashita, Masaaki Okabe, Midori Natsume, Hitoshi Ashida

  Apr. 2012, JOURNAL OF FOOD AND DRUG ANALYSIS, 20, 283 - 287, English

  [Refereed]

  Scientific journal


• Coffee improves insulin-stimulated Akt phosphorylation in liver and skeletal muscle in diabetic KK-A(y) mice.

  Misato Kobayashi, Yuji Matsuda, Hiroshi Iwai, Masanori Hiramitsu, Takashi Inoue, Takao Katagiri, Yoko Yamashita, Hitoshi Ashida, Atsushi Murai, Fumihiko Horio

  2012, Journal of nutritional science and vitaminology, 58(6) (6), 408 - 14, English, Domestic magazine

  [Refereed]

  Scientific journal


• Cacao liquor procyanidin extract improves glucose tolerance by enhancing GLUT4 translocation and glucose uptake in skeletal muscle.

  Yoko Yamashita, Masaaki Okabe, Midori Natsume, Hitoshi Ashida

  2012, Journal of nutritional science, 1, e2 - 9, English, International magazine

  [Refereed]

  Scientific journal


• A black soybean seed coat extract prevents obesity and glucose intolerance by up-regulating uncoupling proteins and down-regulating inflammatory cytokines in high-fat diet-fed mice.

  Yuki Kanamoto, Yoko Yamashita, Fumio Nanba, Tadashi Yoshida, Takanori Tsuda, Itsuko Fukuda, Sachiko Nakamura-Tsuruta, Hitoshi Ashida

  Aug. 2011, Journal of agricultural and food chemistry, 59(16) (16), 8985 - 93, English, International magazine

  [Refereed]

  Scientific journal

• ケルセチン配糖体の単回経口投与による高血糖予防効果

  岡田蕗子, 山下陽子, 北風智也, 北風智也, 吉岡泰淳, 吉岡泰淳, 芦田均

  2023, 日本フードファクター学会学術集会講演要旨集, 28th (CD-ROM)


• ナノモルサイエンスの観点からのケルセチンとその配糖体の高血糖予防効果.

  芦田 均, 山下 陽子

  Nov. 2022, 日本ポリフェノール学会誌, 11, 27 - 32

  [Refereed]


• 概日リズムを考慮したポリフェノールの高血糖抑制効果.

  山下 陽子, 廣直 賢勇, 芦田 均

  Lead, Aug. 2022, 機能性食品と薬理栄養,, 16, 17 - 22

  [Refereed]


• https://doi.org/10.4327/jsnfs.75.77

  山下陽子

  Lead, Apr. 2022, 日本栄養食糧学会誌, 75, 77 - 78, Japanese

  [Refereed][Invited]


• 三つの異なるモデルマウスでのクロダイズポリフェノールによる非アルコール性脂肪性肝疾患の予防効果

  山本美桜, 吉岡泰淳, 北風智也, 山下陽子, 芦田均

  2022, 日本栄養・食糧学会大会講演要旨集, 76th


• Glucose uptake in L6 skeletal muscle cells by 5-hydroxyphenyl-γ-valerolactone, the main microbial metabolite of epigallocatechin gallate

  高垣晶子, 高垣晶子, 吉岡泰淳, 山下陽子, 長野智哉, 池田真規, 芦田均

  2020, 日本農芸化学会大会講演要旨集(Web), 2020


• 6-(メチルスルフィニル)ヘキシルイソチオシアネートはALDH2の誘導を介してアセトアルデヒド毒性から細胞を保護する

  佐澤小桃, 北風智也, 吉岡泰淳, 山下陽子, 芦田均

  2020, 日本栄養・食糧学会近畿支部大会講演要旨集, 59th


• グラブリジンの筋重量に及ぼす効果とその作用機構

  山下陽子, 吉岡泰淳, 久保田祐介, 芦田均

  2019, 日本ポリフェノール学会雑誌, 8(1) (1)


• 黒大豆ポリフェノールの機能性に関する研究.

  YAMASHITA Yoko

  2019, 化学と生物, 57, 146 - 152, Japanese

  [Refereed]

  Introduction scientific journal


• 卵殻膜分解活性を示す微生物由来エラスターゼの検索と特性解析

  横山萌, 竹中慎治, 吉岡泰淳, 山下陽子, 芦田均

  2018, 日本農芸化学会大会講演要旨集(Web), 2018


• クロダイズポリフェノールの非アルコール性脂肪肝疾患予防効果について

  山本美桜, 吉岡泰淳, 山下陽子, 芦田均

  2018, 日本栄養・食糧学会大会講演要旨集, 72nd


• クロダイズの非アルコール性脂肪肝炎予防効果について

  山本美桜, 吉岡泰淳, 山下陽子, 芦田均

  2018, 日本フードファクター学会学術集会講演要旨集, 23rd


• 筋肉への糖取り込み促進効果を持つナツメグ抽出物中の関与成分の検討

  河野龍之進, 前田歩海, 吉岡泰淳, 山下陽子, 芦田均

  2018, 日本農芸化学会大会講演要旨集(Web), 2018


• ピペリンはTRPV1を介してAMPKを活性化し,グルコース取り込みを促進する

  前田歩海, 白鞘大志, 吉岡泰淳, 山下陽子, 芦田均

  2018, 日本農芸化学会関西支部講演会講演要旨集, 505th


• ピペリンはROSを介してAMPKを活性化しグルコース取り込みを促進する

  前田歩海, 吉岡泰淳, 山下陽子, 芦田均

  2018, 日本農芸化学会大会講演要旨集(Web), 2018


• グラブリジンが筋萎縮予防におよぼす効果とその作用機構について

  山下陽子, 吉岡泰淳, 久保田祐介, 芦田均

  2018, 日本栄養・食糧学会近畿支部大会講演要旨集, 57th


• 甘草由来グラブリジンの機能性について

  YAMASHITA YOKO, ASHIDA HITOAHI

  食品資材研究会, 2018, New food industry, 60, 1 - 7, Japanese

  [Invited]

  Introduction scientific journal


• クロダイズの栽培と加工条件による種皮ポリフェノール含量と機能性の変化

  仲村 明日賀, YAMASHITA Yoko, 難波 文男, 戸田 登志也, ASHIDA Hitoshi

  2018, 日本ポリフェノール学会誌, 7, 32 - 40, Japanese

  [Refereed]

  Introduction other


• 黒大豆種皮抽出物の肝臓における食事由来の酸化ストレス抑制効果について

  山本美桜, 吉岡泰淳, 山下陽子, 芦田均

  2017, 日本フードファクター学会学術集会講演要旨集, 22nd


• 微生物由来エラスターゼを用いた卵殻膜の可溶化と生理活性ペプチドの調製

  横山萌, 吉岡泰淳, 山下陽子, 芦田均, 竹中慎治

  2017, 日本生化学会大会(Web), 90th


• 黒大豆種皮ポリフェノールはTGF β1の誘導する肝線維化を阻害する

  吉岡泰淳, 難波文男, 戸田登志也, 山下陽子, 芦田均

  2017, 日本栄養・食糧学会大会講演要旨集, 71st


• 黒大豆種皮由来ポリフェノールによる肝線維化抑制効果

  吉岡泰淳, 李岫, 山下陽子, 芦田均

  2017, 日本酸化ストレス学会学術集会プログラム・抄録集, 70th


• ピペリンによるROSの産生を介したグルコース取り込み促進作用の解明

  前田歩海, 白尾健, 吉岡泰淳, 赤川貢, 山下陽子, 芦田均

  2017, 日本フードファクター学会学術集会講演要旨集, 22nd


• ナツメグ抽出物による筋肉への糖取り込み促進効果について

  河野龍之進, 前田歩海, 吉岡泰淳, 山下陽子, 芦田均

  2017, 日本フードファクター学会学術集会講演要旨集, 22nd


• ピペリンはROSの産生を介してグルコース取り込みを促進する

  前田歩海, 白尾健, 吉岡泰淳, 赤川貢, 山下陽子, 芦田均

  2017, 日本栄養・食糧学会近畿支部大会講演要旨集, 56th


• 黒大豆種皮抽出物は肝臓の酸化ストレスを抑制する

  山本美桜, 吉岡泰淳, 山下陽子, 芦田均

  2017, 日本栄養・食糧学会近畿支部大会講演要旨集, 56th


• 高血糖予防効果を有する緑豆由来ペプチドの探索

  吉岡泰淳, 王きん, 河野光登, 橘伸彦, 山下陽子, 芦田均

  2017, 日本食品科学工学会大会講演集, 64th


• ヒトの健康に寄与する食品成分探索、食物と健康

  ASHIDA Hitoshi, YAMASHITA Yoko

  2017, 神戸女子大学, 165, 3 - 5, Japanese

  [Invited]

  Introduction research institution


• カカオポリフェノールのメタボリックシンドローム予防効果

  YAMASHITA Yoko, ASHIDA Hitoshi

  日本油化学会, 2017, オレオサイエンス, 17(10) (10), 467 - 474, Japanese

  [Refereed][Invited]

  Introduction scientific journal


• グラブリジン含有組成物のマウスにおける筋肉増強作用機構

  吉岡泰淳, 山下陽子, 岸田英之, 中川格, 芦田均

  2016, 日本栄養・食糧学会大会講演要旨集, 70th


• ケルセチンとイソラムネチンによる筋肉細胞へのグルコース取り込み促進効果

  芦田均, 姜浩, 吉岡泰淳, 山下陽子

  2016, ビタミン, 90(4) (4)


• ピペリンによる血糖調節作用の機構解明

  前田歩海, 吉岡泰淳, 山下陽子, 赤川貢, 芦田均

  2016, 日本食品科学工学会大会講演集, 63rd


• カルコンを高含有するアシタバ抽出物の肥満予防効果

  芦田均, 張天順, 山下陽子, 山本憲朗

  2016, 日本栄養・食糧学会大会講演要旨集, 70th


• 日本ヨモギエタノール抽出物が糖尿病モデルマウスにおよぼす影響

  辻愛, 山本憲朗, 山下陽子, 芦田均

  2016, 日本農芸化学会関西支部講演会講演要旨集, 496th


• プロシアニジンの機能性

  YAMASHITA Yoko, ASHIDA Hitoshi

  公益社団法人 日本農芸化学会, 2016, 化学と生物, 54(10) (10), 747 - 752, Japanese

  [Refereed]

  Introduction scientific journal


• 黒豆種皮含有ポリフェノールは酸化的DNA損傷を抑制する

  吉岡泰淳, 李岫, 難波文男, 戸田登志也, 山下陽子, 芦田均, 芦田均

  2015, 日本栄養・食糧学会近畿支部大会講演要旨集, 54th


• 食品成分による耐糖の異常の改善について

  ASHIDA HITOSHI, 山下 陽子

  日本食品機械研究会, 2015, 食品加工技術, 35(1) (1), 31 - 35, Japanese

  [Invited]

  Introduction scientific journal


• プロシアニジンの新たな生体調節機能

  YAMASHITA Yoko, ASHIDA Hitoshi

  2014, 化学と生物, 52(8) (8), 493 - 494, Japanese

  [Refereed]

  Introduction scientific journal


• 陽子先生の食育コラム第4回：食卓に真心を・・・

  YAMASHITA Yoko

  Jan. 2014, 新鮮mini情報(農林水産省神戸地域センター情報誌) 平成26年1月号, Japanese

  Introduction other


• 陽子先生の食育コラム第3回：発酵食品に真心を・・・

  YAMASHITA Yoko

  Dec. 2013, 新鮮mini情報(農林水産省神戸地域センター情報誌) 平成25年12月号, Japanese

  Introduction other


• 陽子先生の食育コラム第2回：”食育は真心”の主役は微生物 微生物に真心を・・・

  YAMASHITA Yoko

  Nov. 2013, 新鮮mini情報(農林水産省神戸地域センター情報誌) 平成25年11月号, Japanese

  Introduction other


• 陽子先生の食育コラム第１回：食育は、”風土に根ざすまごころの営み”という視点を大切に・・・

  YAMASHITA Yoko

  Oct. 2013, 新鮮mini情報(農林水産省神戸地域センター情報誌) 平成25年10月号, Japanese

  Introduction other


• フラボノイド類のグルコーストランスポーターを介した血糖値調節作用について

  YAMASHITA Yoko, ASHIDA Hitoshi

  FFIジャーナル編集委員会, 2013, FFIジャーナル, 218(2) (2), 158 - 165, Japanese

  Introduction commerce magazine


• ポリフェノールによるグルコーストランスポーターの機能調節

  YAMASHITA Yoko, YAMAMOTO Norio, ASHIDA Hitoshi

  THE VITAMIN SOCIETY OF JAPAN, 2012, ビタミン学会誌, 86(3) (3), 163 - 173, Japanese

  [Refereed]

  Introduction scientific journal

• ポリフェノールの科学 : 基礎化学から健康機能まで 8 メタボリックシンドロームの予防

  寺尾, 純二, 下位, 香代子, 越阪部, 奈緒美, 榊原, 啓之, 中村, 宜督, 三好, 規之, 室田, 佳恵子

  Joint work, 朝倉書店, Nov. 2023, Japanese, ISBN: 9784254103038


• "Health beneficial functions of black soybean polyphenols" in "Food Biotechnology" 3rd Edition

  YAMASHITA Yoko, YOSHIOKA Yasykiyo, ASHIDA Hitoshi

  Joint work, CRC Press, 2019, English

  Scholarly book


• 黒大豆ポリフェノール 「血流改善成分の開発と応用」

  YAMASHITA Yoko, ASHIDA Hitoshi

  Joint work, シーエムシー出版, 2018, Japanese

  Scholarly book


• 第Ⅱ編食品素材としてのポリフェノールの研究開発；４．カカオポリフェノールの抗肥満効果『ポリフェノール: 『機能性成分研究開発の最新動向』

  ASHIDA Hitoshi, YAMASHITA Yoko

  Joint work, シーエムシー出版, 2016, Japanese

  Scholarly book


• 第12章 プロシアニジンによる血糖ならびに脂質代謝調節「食品因子による栄養機能制御」pp. 177-195，日本栄養・食糧学会監修，芦田 均，立花 宏文，原 博 責任編集

  YOKO YAMASHITA, ASHIDA HITOSHI

  Joint work, 建帛社, May 2015, Japanese

  Scholarly book

• 生理的濃度域でのルテオリンの投与タイミングの違いによる薬物代謝酵素の発現について

  MAKIYAMA Atsushi, KITAKAZE Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

  第57回日本栄養・食糧学会近畿支部大会, Dec. 2018, Japanese, Nara, Domestic conference

  Oral presentation


• レチノイン酸誘発性トランスグルタミナーゼ2は分泌タンパク質として筋肥大を誘発する

  YOSHIKAWA Miki, KITAKAZE Tomoya, HARADA Naoki, INUI Hiroshi, YAMASHITA Yoko, ASHIDA Hitoshi, YAMAJI Ryoichi

  第57回日本栄養・食糧学会近畿支部大会, Dec. 2018, Japanese, Nara, Domestic conference

  Oral presentation


• グラブリジンが筋萎縮予防におよぼす影響とその作用機構について

  YAMASHITA Yoko, YOSHIOKA Yasukiyo, KUBOTA Yusuke, ASHIDA Hitoshi

  第57回日本栄養・食糧学会近畿支部大会, Dec. 2018, Japanese, Nara, Domestic conference

  Oral presentation


• アシタバ由来カルコンによるステロイド性筋萎縮の抑制効果について

  SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

  第57回日本栄養・食糧学会近畿支部大会, Dec. 2018, Japanese, Domestic conference

  Oral presentation


• B-ring catechol structure of flavonol os important for promotion of glucose uptake into muscle cells

  AYA Megumi, SHIRASAYA Daishi, YAMASHITA Yoko, ASHIDA Hitoshi

  若手フロンティア研究会2018, Dec. 2018, Japanese, Kobe, Domestic conference

  Poster presentation


• Ashidtaba chalcons prevent steroid-induced musle atrophy

  SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

  若手フロンティア研究会2018, Dec. 2018, Japanese, Kobe, Domestic conference

  Poster presentation


• The effect of black soybean on vascular function

  YAMASHITA Yoko, NAKAMURA Asuka, Asuka Nakamura, WANG Liuqing, SAITOU Shizuka, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi

  4th International Conference on Pharma and Food (ICPF2018), Nov. 2018, English, Shizuoka, International conference

  Poster presentation


• Hepa1c1c7細胞における芳香族炭化水素受容体の活性化がBMAL1の発現に与える影響

  KITAKAZE Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

  第41回日本分子生物学会年会, Nov. 2018, Japanese, Yokohama, Domestic conference

  Poster presentation


• Health beneficial functions of black soybean polyphenols

  ASHIDA Hitoshi, YAMASHITA Yoko

  4th International Conference on Pharma and Food (ICPF2018), Nov. 2018, English, Shizuoka, International conference

  [Invited]

  Invited oral presentation


• Differences in polyphenol content of black soybeans among different varieties and cultivation years

  LI Weijing, YAMASHITA Yoko, ASHIDA Hitoshi

  JSBBA Kansai 5th Student Forum, Nov. 2018, English, Nara, International conference

  Poster presentation


• B-ring catechol structure of flavonol is important for prevention of postprandial hyperglycemia via promotion of glucose uptake into muscle cells

  AYA Megumi, YAMASHITA Yoko, ASHIDA Hitoshi

  The31th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2018 Tsukuba), Nov. 2018, English, Tsukuba, International conference

  Poster presentation


• Ashiba chalcons prevent dexamethasone-induced muscle protein degradation

  SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

  The31th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2018 Tsukuba), Nov. 2018, English, Tsukuba, International conference

  Poster presentation


• The preventive effect of Italian propolis on postprandial hyperglycemia

  SHIRASAYA Daishi, YAMASHITA Yoko, ASHIDA Hitoshi

  International Conference and Exhibition on Nutraceuticals and Functional Foods 2018, Oct. 2018, English, Vancouver, International conference

  Poster presentation


• The effect of black soybean on vascular function in human trial

  NAKAMURA Asuka, WANG Liuqing, NAMBA Fumio, SAITO Shizuka, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

  International Conference and Exhibition on Nutraceuticals and Functional Foods 2018, Oct. 2018, English, Vancouver, International conference

  Poster presentation


• Preventive effect of black soybean polyphenols on non-alcoholic steatohepatitis

  YAMAMOTO Mio, YOSHIOKA Yasukiyo, YAMASHITA Yoko, ASHIDA Hitoshi

  International Conference and Exhibition on Nutraceuticals and Functional Foods 2018, Oct. 2018, English, Vancouver, International conference

  Poster presentation


• Luteolin induces expression of phase II drug metabolizing enzymes by modulating Nrf2-ARE pathway in HepG2 cells

  KITAKAZE Tomoya, MAKIYAMA Atsushi, YAMASHITA Yoko, ASHIDA Hitoshi

  International Conference and Exhibition on Nutraceuticals and Functional Foods 2018, Oct. 2018, English, Vancouver, International conference

  Poster presentation


• Chalcones derived from ashitaba prevent dexamethasone-induced muscle protein degradation

  SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

  International Conference and Exhibition on Nutraceuticals and Functional Foods 2018, Oct. 2018, English, Vancouver, International conference

  Poster presentation


• 芳香族炭化水素受容体の活性化による時計遺伝子発現の変化に対する食品因子の作用

  KITAKAZE Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

  Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

  Poster presentation


• 生理的濃度域のルテオリンによるNrf2-ARE経路を介した薬物代謝酵素の発現調節機構

  MAKIYAMA Atsushi, KITAKAZE Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

  2018年度日本農芸化学会関西支部大会, Sep. 2018, Japanese, Kyoto, Domestic conference

  Oral presentation


• 黒大豆ポリフェノール含量の品種並びに栽培年度での差について

  LI Weijing, YAMASHITA Yoko, ASHIDA Hitoshi

  Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

  Poster presentation


• 黒大豆ポリフェノールの血管機能向上効果とその作用機構の解明

  DOMAE Chiaki, WANG Liuqing, NAMBA Fumio, SAITOU Shizuka, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

  Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

  Poster presentation


• 黒大豆ポリフェノールの吸収、代謝、分布と排泄および血管機能に及ぼす影響に関する研究

  WANG Liuqing, YAMASHITA Yoko, NAKAMURA Asuka, NAKAGAWA Junichi, NAMBA Fumio, SAITOU Shizuka, TODA Toshiya, ASHIDA Hitoshi

  2018年度日本農芸化学会関西支部大会, Sep. 2018, Japanese, Kyoto, Domestic conference

  Oral presentation


• 黒大豆クッキー摂取が欠陥機能に与える効果（ヒト介入試験）の検証

  NAKAMURA Asuka, WANG Liuqing, NAMBA Fumio, SAITOU Shizuka, MIKI Fumio, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

  Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

  Oral presentation


• ピペリンはTRPV1を介してAMPKを活性化し，グルコース取り込みを促進する

  MAEDA Ayumi, SHIRASAYA Daishi, YOSHIOKA Yasukiyo, YAMASHITA Yoko, ASHIDA Hitoshi

  2018年度日本農芸化学会関西支部大会, Sep. 2018, Japanese, Kyoto, Domestic conference

  Oral presentation


• シカ肉の肝エネルギー代謝に関する研究

  TIAN Xiaokuo, YAMASHITA Yoko, ASHIDA Hitoshi

  Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

  Poster presentation


• クロダイズ種皮由来ポリフェノールによる脂肪蓄積抑制効果とその作用機序

  KOIKE Akihiro, YAMASHITA Yoko, ASHIDA Hitoshi, NAMBA Fumio, TODA Toshiya

  Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

  Poster presentation


• クロダイズの非アルコール性脂肪肝炎予防効果について

  YAMAMOTO Mio, YOSHIOKA Yasykiyo, YAMASHITA Yoko, ASHIDA Hitoshi

  Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

  Poster presentation


• アシタバ由来カルコンによる筋萎縮抑制効果の検証とその分子機構の解明

  SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

  Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

  Oral presentation


• Prevention mechanisms of glucose intolerance and obesity by enzymatically modified isoquercitrin and quercetin in mice

  JIANG Hao, YOSHIOKA Yasukiyo, YUAN Sihao, YAMASHITA Yoko, ASHIDA Hitoshi

  Food Congress 2018, Sep. 2018, English, Kyoto, Domestic conference

  Oral presentation


• Nrf2-ARE経路を介した薬物代謝酵素の発現調節機構に生理的濃度のルテオリンが与える影響

  MAKIYAMA Atsushi, KITAKAZE Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

  Food Congress 2018, Sep. 2018, Japanese, Kyoto, Domestic conference

  Poster presentation


• 健常者を対象とした黒大豆加工品摂取による血管機能向上効果（ヒト試験）の検証

  NAKAMURA Asuka, WANG Liuqing, NAMBA Fumio, SAITOU Shizuka, MIKI Fumio, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

  日本食品工学会第65回大会, Aug. 2018, Japanese, Sendai, Domestic conference

  Oral presentation


• クロダイズが血管機能に及ぼす影響とその作用機構の解明

  DOMAE Chiaki, WANG Liuqing, NAMBA Fumio, SAITOU Shizuka, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

  日本食品工学会第65回大会, Aug. 2018, Japanese, Sendai, Domestic conference

  Oral presentation


• カカオポリフェノールが時計遺伝子の発現に及ぼす影響について

  YAMASHITA Yoko, HUANG shujiao, MITSUHASHI Yuji, ASHIDA Hitoshi

  第5回時間栄養科学研究会, Aug. 2018, Japanese, Saitama, Domestic conference

  Oral presentation


• 芳香族炭化水素受容体の活性化と抑制作用をもつ食品成分による時計遺伝子の発現変動について

  KITAKAZE Tomoya, MAKIYAMA Atsushi, YAMASHITA Yoko, ASHIDA Hitoshi

  第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

  Oral presentation


• 生理的濃度のルテオリンによるNrf2を介した薬物代謝酵素の発現調節機構

  MAKIYAMA Atsushi, KITAKAZE Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

  第71回日本酸化ストレス学会第18回日本NO学会合同学術集会, May 2018, Japanese, Kyoto, Domestic conference

  Poster presentation


• 黒大豆の血管機能向上効果とその作用機構

  DOMAE Chiaki, WANG Linqing, NAMBA Fumio, SAITOU Shizuka, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

  第71回日本酸化ストレス学会第18回日本NO学会合同学術集会, May 2018, Japanese, Kyoto, Domestic conference

  Poster presentation


• 健常者を対象としたクロダイズ加工品摂取による血管機能向上効果(ヒト試験)の検証

  NAKAMURA Asuka, WANG Liuqing, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

  第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

  Oral presentation


• フラボノールのB環カテコール構造が筋肉細胞へのグルコース取り込み促進を介した食後高血糖予防効果に重要である

  AYA Megumi, SHIRASAYA Daishi, YAMASHITA Yoko, ASHIDA Hitoshi

  第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

  Oral presentation


• トランスグルタミナーゼ2は骨格筋のタンパク質合成を促進する

  YOSHIKAWA Miki, KITAKAZE Tomoya, HARADA Naoki, INUI Hiroshi, YAMASHITA Yoko, ASHIDA Hitoshi, YAMAJI Ryoichi

  第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

  Oral presentation


• ケルセチンとその配糖体が血糖調節に及ぼす効果

  YAMASHITA Yoko, JIANG Hao, SHIRASAYA Daishi, AYA Megumi, ASHIDA Hitoshi

  第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

  Oral presentation


• クロダイズ種皮由来ポリフェノールによる脂肪蓄積の抑制とその作用機構

  KOIKE Akihiro, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

  第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

  Oral presentation


• クロダイズポリフェノールの非アルコール性脂肪肝疾患予防効果について

  YOSHIOKA Yasukiyo, YAMASHITA Yoko, ASHIDA Hitoshi

  第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

  Oral presentation


• カカオポリフェノールの肥満・高血糖予防について

  ASHIDA Hitoshi, YAMASHITA Yoko

  第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

  [Invited]

  Nominated symposium


• アシタバカルコンによる筋タンパク質分解抑制効果の検証とその分子機構解明myotubes

  SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

  第72回 日本栄養・食糧学会, May 2018, Japanese, Okayama, Domestic conference

  Oral presentation


• 卵殻膜分解活性を示す微生物由来エラスターゼの検索と特性解析

  YOKOYAMA Moe, TAKENAKA Shinji, YOSHIOKA Yasykiyo, YAMASHITA Yoko, ASHIDA Hitoshi

  日本農芸化学会2018年度大会, Mar. 2018, Japanese, Nagoya, Domestic conference

  Oral presentation


• 生理的濃度のルテオリンはNrf2-Keap1経路を薬物代謝第II相酵素の発現量を調節する

  KITAKAZE Tomoya, MAKIYAMA Atsushi, YAMASHITA Yoko, ASHIDA Hitoshi

  日本農芸化学会2018年度大会, Mar. 2018, Japanese, Nagoya, Domestic conference

  Oral presentation


• 筋肉への糖取り込み促進効果を持つナツメグ抽出物中の関与成分の検討

  KONO Ryunoshin, MAEDA Ayumi, YOSHIOKA Yasykiyo, YAMASHITA Yoko, ASHIDA Hitoshi

  日本農芸化学会2018年度大会, Mar. 2018, Japanese, Nagoya, Domestic conference

  Oral presentation


• プロシアニジンの高血糖抑制効果とその作用機構についての比較検討

  YAMASHITA Yoko, TSUDA Takanori, ASHIDA Hitoshi

  日本農芸化学会2018年度大会, Mar. 2018, Japanese, Nagoya, Domestic conference

  Oral presentation


• フラボノールによるグルコース取り込み促進効果にはB環のカテコール構造が重要である

  AYA Megumi, SHIRSAYA Daishi, YAMASHITA Yoko, ASHIDA Hitoshi

  日本農芸化学会2018年度大会, Mar. 2018, Japanese, Nagoya, Domestic conference

  Oral presentation


• ピペリンはROSを介してAMPKを活性化しグルコース取り込みを促進する

  MAEDA Ayumi, YOSHIOKA Yasykiyo, YAMASHITA Yoko, ASHIDA Hitoshi

  日本農芸化学会2018年度大会, Mar. 2018, Japanese, Nagoya, Domestic conference

  Oral presentation


• Kaempferol ameliorates glucose uptake via AMPK- and JAK-STAT-pathways in L6 myotubes

  JIANG Hao, YOSHIOKA Yasukiyo, YAMASHITA Yoko, ASHIDA Hitoshi

  日本農芸化学会2018年度大会, Mar. 2018, Japanese, Nagoya, Domestic conference

  Oral presentation


• 野菜の機能性と健康効果

  YAMASHITA Yoko

  神河有機農業教室, Dec. 2017, Japanese, Hyogo, Domestic conference

  Public discourse


• 腸内環境からみた黒大豆ポリフェノールによる健康長寿

  ASHIDA Hitoshi, YAMASHITA Yoko

  第15回日本機能性食品医用学会, Dec. 2017, Japanese, Tokyo, Domestic conference

  [Invited]

  Invited oral presentation


• 食と健康に関する最新研究知見とそれを応用した食育の実践

  YAMASHITA Yoko

  中播磨地区学校給食研究協議会 栄養管理部会研修会, Dec. 2017, Japanese, Hyogo, Domestic conference

  Public discourse


• β-カロテンが誘導する分泌型タンパク質は骨格筋のタンパク質合成を増加させる

  KITAKAZE Tomoya, HARADA Naoki, YAMASHITA Yoko, ASHIDA Hitoshi, YAMAJI Ryoichi

  第22回日本フードファクター学会学術集会, Dec. 2017, Japanese, Fujisawa, Domestic conference

  Oral presentation


• ルテオリンによるNrf2を介した薬物代謝酵素の誘導調節機構

  MAKIYAMA Atsushi, KITAKAZE Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

  第22回日本フードファクター学会学術集会, Dec. 2017, Japanese, Fujisawa, Domestic conference

  Oral presentation


• プロポリス由来成分による高血糖予防効果の作用機序解明

  SHIRASAYA Daishi, IKADA Masaki, YAMASHITA Yoko, GENOVESE Salvatore, ASHIDA Hitoshi

  第22回日本フードファクター学会学術集会, Dec. 2017, Japanese, Fujisawa, Domestic conference

  Oral presentation


• ピペリンによるROSの産生を介したグルコース取り込み促進作用の解明

  MAEDA Ayumi, SHIRAO Takeshi, YOSHIOKA Yasykiyo, AKAGAWA Mitsugu, YAMASHITA Yoko, ASHIDA Hitoshi

  第22回日本フードファクター学会学術集会, Dec. 2017, Japanese, Fujisawa, Domestic conference

  Oral presentation


• ナツメグ抽出物による筋肉への糖取り込み促進効果について

  KONO Ryunoshin, MAEDA Ayumi, YOSHIOKA Yasykiyo, YAMASHITA Yoko, ASHIDA Hitoshi

  第22回日本フードファクター学会学術集会, Dec. 2017, Japanese, Fujisawa, Domestic conference

  Oral presentation


• アシタバカルコンによるデキサメタゾン誘導性筋タンパク質分解抑制効果に関する研究

  SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

  第22回日本フードファクター学会学術集会, Dec. 2017, Japanese, Fujisawa, Domestic conference

  Oral presentation


• 4-Hydroxyderricinによる筋タンパク質分解抑制効果の検証とその分子機構解明

  SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

  神戸大学若手フロンティア研究会2017, Dec. 2017, Japanese, Kobe, Domestic conference

  Poster presentation


• 黒大豆種皮抽出物は肝臓の酸化ストレスを抑制する

  YAMAMOTO Mio, YOSHIOKA Yasykiyo, YAMASHITA Yoko, ASHIDA Hitoshi

  第56回日本栄養・食糧学会近畿支部大会, Nov. 2017, Japanese, Ohtsu, Domestic conference

  Oral presentation


• 黒大豆の機能性を再発見～環境創造型農業による黒大豆の研究～

  YAMASHITA Yoko

  たじま優しい有機農業講座, Nov. 2017, Japanese, Hyogo, Domestic conference

  Public discourse


• 黒大豆の機能性を再発見～環境創造型農業による黒大豆の研究～

  YAMASHITA Yoko

  おおや有機農業の学校, Nov. 2017, Japanese, Hyogo, Domestic conference

  Public discourse


• プロポリス由来成分による高血糖予防効果の解明

  SHIRASAYA Daishi, IKADA Masaki, NAGANO Tomoya, YAMASHITA Yoko, GENOVESE Salvatore, ASHIDA Hitoshi

  第56回日本栄養・食糧学会近畿支部大会, Nov. 2017, Japanese, Ohtsu, Domestic conference

  Oral presentation


• ピペリンは ROS の産生を介してグルコース取り込みを促進する

  MAEDA Ayumi, SHIRAO Takeshi, YOSHIOKA Yasykiyo, AKAGAWA Mitsugu, YAMASHITA Yoko, ASHIDA Hitoshi

  第56回日本栄養・食糧学会近畿支部大会, Nov. 2017, Japanese, Ohtsu, Domestic conference

  Oral presentation


• アシタバカルコンによる筋タンパク質分解抑制効果に関する研究

  SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

  第56回日本栄養・食糧学会近畿支部大会, Nov. 2017, Japanese, Ohtsu, Domestic conference

  Oral presentation


• The elucidating hyperglycemia preventive effect by propolis compounds

  SHIRASAYA Daishi, IKEDA Masaki, YAMASHITA Yoko, GENOVESE Salvatore, ASHIDA Hitoshi

  JSBBA Kansai 4th Student Forum, Nov. 2017, English, Kobe, Domestic conference

  Poster presentation


• Cacao polypenols prevents hyperglycemia under the circadian clock genes

  HUANG Shujiao, YAMASHITA Yoko, ASHIDA Hitoshi

  JSBBA Kansai 4th Student Forum, Nov. 2017, English, Kobe, Domestic conference

  Poster presentation


• Procyanidin prevents hyperglycemia through promoting translocation of glucose transporter 4 in muscle cells

  YAMASHITA Yoko, ASHIDA Hitoshi

  8th International Conference on Polyphenols and Health, Oct. 2017, English, Quebec, International conference

  Oral presentation


• Glabridin inhibits glucocorticoid-induced muscle atrophy in C2C12 cells and C57BL6J mice

  ASHIDA Hitoshi, KUBOTA Yusuke, YOSHIOKA Yasukiyo, YAMASHITA Yoko

  8th International Conference on Polyphenols and Health, Oct. 2017, English, Quebec, International conference

  Oral presentation


• Fisetin, but not robinetin, promotes glucose uptake in skeletal muscle cells

  AYA Megumi, YAMASHITA Yoko, ASHIDA Hitoshi

  8th International Conference on Polyphenols and Health, Oct. 2017, English, Quebec, International conference

  Oral presentation


• Enzymatically Synthesized Glycogen Inhibits Colitis Through Decreasing Oxidative Stress

  ASHIDA Hitoshi, MITANI Takakazu, YOSHIOKA Yasukiyo, YAMASHITA Yoko, FURUYASIKI Takashi

  The 10th International Conference and Exhibition on Nutraceuticals and Functional Foods (ISNFF2017),, Oct. 2017, English, Gunsan, International conference

  [Invited]

  Invited oral presentation


• Curcumin and its derivatives inhibit TCDD-induced expression of drug metabolizing enzymes

  ASHIDA Hitoshi, NAKAI Rika, YAMASHITA Yoko

  8th International Conference on Polyphenols and Health, Oct. 2017, English, Quebec, International conference

  Poster presentation


• Black soybean seed coat polyphenols prevent fibrosis in the liver of mice via suppression of lipid peroxidation and inhibition of TGF-b Smads signaling pathway

  YOSHIOKA Yasukiyo, LI Xiu, YAMASHITA Yoko, ASHIDA Hitoshi

  8th International Conference on Polyphenols and Health, Oct. 2017, English, Quebec, International conference

  Poster presentation


• 発酵茶が脂肪蓄積と高血糖予防に及ぼす効果とその作用機構

  YAMASHITA Yoko, ASHIDA Hitoshi

  日本農芸化学会 関西・中四国・西日本支部 2017年度合同大阪大会（第49回講演会）, Sep. 2017, Japanese, Sakai, Domestic conference

  Oral presentation


• 食と健康に関する最新研究知見とそれを応用した食育の実践

  YAMASHITA Yoko

  日本教職員組合栄養教職員部近畿ブロック学習会, Sep. 2017, Japanese, Hyogo, Domestic conference

  Public discourse


• 黒ダイズ種皮抽出物は四塩化炭素の誘導する肝の線維化を抑制する

  YOSHIOKA Yasukiyo, RI Xiu, YAMASHITA Yoko, ASHIDA Hitoshi

  フードサイエンスフォーラム第23回学術集会, Sep. 2017, Japanese, Miyazaki, Domestic conference

  Oral presentation


• マウスにおけるプロシアニジンの体内動態

  WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

  日本農芸化学会 関西・中四国・西日本支部 2017年度合同大阪大会（第49回講演会）, Sep. 2017, Japanese, Sakai, Domestic conference

  Oral presentation


• ヒト介入試験による黒大豆の血管機能向上効果について

  YAMASHITA Yoko, ASHIDA Hitoshi

  フードサイエンスフォーラム第23回学術集会, Sep. 2017, Japanese, Miyazaki, Domestic conference

  Oral presentation


• アシタバカルコン4-hydroxyderricinはデキサメタゾン誘導性の筋タンパク質分解を抑制する

  SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

  フードサイエンスフォーラム第23回学術集会, Sep. 2017, Japanese, Miyazaki, Domestic conference

  Oral presentation


• 煎り黒豆を継続摂取したヒト血中および尿中のポリフェノール濃度.

  WANG Liuqing, YAMASHITA Yoko, NAMBA Fumio, SAITOU Shizuka, TODA Toshiya, ASHIDA Hitoshi

  日本食品科学工学会 第64回大会, Aug. 2017, Japanese, Syonan, Domestic conference

  Oral presentation


• 煎り黒大豆摂取がヒトの血管機能向上に及ぼす効果

  YAMASHITA Yoko, WANG Liuqing, NAMBA Fumio, SAITOU Shizuka, TODA Toshiya, ASHIDA Hitoshi

  日本食品科学工学会 第64回大会, Aug. 2017, Japanese, Syonan, Domestic conference

  Oral presentation


• 煎り黒大豆の摂取によるヒト血管機能の向上

  ASHIDA Hitoshi, YAMASHITA Yoko, NAMBA Fumio, TODA Toshiya

  SIP「次世代機能性農林水産物・食品の開発」報告会, Aug. 2017, Japanese, Tokyo, Domestic conference

  [Invited]

  Nominated symposium


• 黒大豆種皮抽出物の血管内皮機能改善作用

  SAITOU Shizuka, NAMBA Fumio, ASHIDA Hitoshi, YAMASHITA Yoko, TODA Toshiya

  日本食品科学工学会 第64回大会, Aug. 2017, Japanese, Syonan, Domestic conference

  Oral presentation


• 黒大豆ポリフェノールによる非アルコール性脂肪肝疾患の予防効果と作用機構の解明

  YOSHIOKA Yasukiyo, YAMASHITA Yoko, ASHIDA Hitoshi

  第11回日本ポリフェノール学会年次大会, Aug. 2017, Japanese, Kanazawa, Domestic conference

  Oral presentation


• 高血糖予防効果を有する緑豆由来ペプチドの探索

  YOSHIOKA Yasukiyo, WANG Xin, KOHNA Mitsutaka, TACHIBANA Nobuhiko, YAMASHITA Yoko, ASHIDA Hitoshi

  日本食品科学工学会 第64回大会, Aug. 2017, Japanese, Syonan, Domestic conference

  Oral presentation


• 加工過程によるクロダイズポリフェノール成分の変化とその生体機能性について

  NAKAMURA Asuka, LIUQING Wang, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

  第11回日本ポリフェノール学会年次大会, Aug. 2017, Japanese, Kanazawa, Domestic conference

  Oral presentation


• プロポリス由来prenyloxyphenylpropanoidsによる糖取り込み促進作用の作用機序解明

  SHIRASAYA Daishi, NAGANO Tomoya, IKEDA Masaki, YAMASHITA Yoko, Salvatore Genovese, ASHIDA Hitoshi

  第11回日本ポリフェノール学会年次大会, Aug. 2017, Japanese, Kanazawa, Domestic conference

  Oral presentation


• フィセチンとロビネチンの筋肉細胞への取り込み量と糖取り込み促進効果の関係について

  AYA Megumi, YAMASHITA Yoko, ASHIDA Hitoshi

  第11回日本ポリフェノール学会年次大会, Aug. 2017, Japanese, Kanazawa, Domestic conference

  Oral presentation


• クロダイズ種皮由来ポリフェノールによる高血糖・肥満抑制効果とその作用機構

  KOIKE Akihiro, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

  第11回日本ポリフェノール学会年次大会, Aug. 2017, Japanese, Kanazawa, Domestic conference

  Oral presentation


• カカオポリフェノール抽出物の投与タイミングの違いによる血糖調節と時計遺伝子に及ぼす影響

  HUANG shujiao, MITSUHASHI Yuji, ASHIDA Hitoshi, YAMASHITA Yoko

  第11回日本ポリフェノール学会年次大会, Aug. 2017, Japanese, Kanazawa, Domestic conference

  Oral presentation


• カカオポリフェノール抽出物の投与タイミングの違いが高血糖抑制作用と時計遺伝子に及ぼす影響

  HUANG Shujiao, MITSUHASHI Yuji, ASHIDA Hitoshi, YAMASHITA Yoko

  日本食品科学工学会 第64回大会, Aug. 2017, Japanese, Syonan, Domestic conference

  Oral presentation


• アシタバカルコン 4-hydroxyderricinによる筋萎縮抑制効果について

  SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

  第11回日本ポリフェノール学会年次大会, Aug. 2017, Japanese, Kanazawa, Domestic conference

  Oral presentation


• The effect of kaempferol on glucose uptake in L6 myotubes

  JIANG Hao, YOSHIOKA Yasukiyo, Yoko Yamashita, Hitoshi Ashida

  日本食品科学工学会 第64回大会, Aug. 2017, English, Syonan, Domestic conference

  Oral presentation


• ルテオリンによるNrf2を介した薬物代謝酵素の誘導調節

  MAKIYAMA Atsushi, JIANG Songyan, KITAKAZE Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

  日本動物細胞工学会2017年度大会(JAACT2017), Jul. 2017, Japanese, Matsuyama, Domestic conference

  Poster presentation


• プロポリス由来成分による筋肉細胞への糖取り込み作用について

  SHIRASAYA Daishi, NAGANO Tomoya, IKEDA Masaki, YAMASHITA Yoko, Salvatore Genovese, ASHIDA Hitoshi

  日本動物細胞工学会2017年度大会(JAACT2017), Jul. 2017, Japanese, Matsuyama, Domestic conference

  Poster presentation


• フィセチンとロビネチンによる筋肉細胞の糖取り込み促進効果について

  AYA Megumi, YAMASHITA Yoko, ASHIDA Hitoshi

  日本動物細胞工学会2017年度大会(JAACT2017), Jul. 2017, Japanese, Matsuyama, Domestic conference

  Poster presentation


• アシタバカルコン4-hydroxyderricinによる筋萎縮抑制効果について

  SAMUKAWA Yumi, YAMASHITA Yoko, ASHIDA Hitoshi

  日本動物細胞工学会2017年度大会(JAACT2017), Jul. 2017, Japanese, Matsuyama, Domestic conference

  Poster presentation


• The effect of kaempferol on glucose uptake in L6 myotubes

  JIANG Hao, YOSHIOKA Yasukiyo, Yoko Yamashita, Hitoshi Ashida

  日本動物細胞工学会2017年度大会(JAACT2017), Jul. 2017, English, Matsuyama, Domestic conference

  Poster presentation


• 黒大豆種皮由来ポリフェノールによる肝線維化抑制効果

  YOSHIOKA Yasukiyo, RI Xiu, YAMASHITA Yoko, ASHIDA Hitoshi

  第70回日本酸化ストレス学会学術集会, Jun. 2017, Japanese, Tsukuba, Domestic conference

  Oral presentation


• FisetinとRobinetinによる筋肉細胞のGLUT4膜移行促進効果の差異について

  AYA Megumi, YAMASHITA Yoko, ASHIDA Hitoshi

  日本ビタミン学会第69回大会, Jun. 2017, Japanese, Yokohama, Domestic conference

  Oral presentation


• 黒大豆種皮ポリフェノールはTGFβ1の誘導する肝線維化を阻害する

  YOSHIOKA Yasukiyo, NAMBA Fumio, TODA Toshiya, YAMASHITA Yoko, ASHIDA Hitoshi

  第71回日本栄養・食糧学会, May 2017, Japanese, Okinawa, Domestic conference

  Oral presentation


• 黒大豆の機能性を再発見～環境創造型農業による黒大豆の研究～

  YAMASHITA Yoko, ASHIDA Hitoshi

  神付有機農業教室, May 2017, Japanese, Hyogo, Domestic conference

  Public discourse


• グラブリジンの高血糖抑制効果について

  YAMASHITA Yoko, NAKAGAWA Kaku, KISHIDA Hideyuki, ASHIDA Hitoshi

  第71回日本栄養・食糧学会, May 2017, Japanese, Okinawa, Domestic conference

  Oral presentation


• 黒大豆種皮抽出物の血管内皮機能改善作用

  NAMBA Fumio, SAITOU Shizuka, TODA Toshiya, YAMASHITA Yoko, ASHIDA Hitoshi

  日本農芸化学会2017年度大会, Mar. 2017, Japanese, Kyoto, Domestic conference

  Oral presentation


• 黒大豆の生体機能性について

  YAMASHITA Yoko, NAMBA Fumio, SAITOU Shizuka, TODA Toshiya, ASHIDA Hitoshi

  日本農芸化学会2017年度大会, Mar. 2017, Japanese, Kyoto, Domestic conference

  Oral presentation


• 黒大豆の加工によるポリフェノール含量と機能性の変化

  NAKAMURA Asuka, Liuqing Wang, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

  日本農芸化学会2017年度大会, Mar. 2017, Japanese, Kyoto, Domestic conference

  Oral presentation


• ナツメグ抽出物による筋肉への糖取り込み促進効果について

  KONO Ryunoshin, MAEDA Ayumi, YOSHIOKA Yasukiyo, IKEDA Masaki, YAMASHITA Yoko, ASHIDA Hitoshi

  日本農芸化学会2017年度大会, Mar. 2017, Japanese, Kyoto, Domestic conference

  Oral presentation


• ケンフェロールとルテオリンによる薬物代謝酵素発現調節機構について

  NAKAI Rika, YAMASHITA Yoko, ASHIDA Hitoshi

  日本農芸化学会2017年度大会, Mar. 2017, Japanese, Kyoto, Domestic conference

  Oral presentation


• カカオポリフェノールによるエネルギー代謝促進効果：投与タイミングと時計遺伝子発現

  MITSUHASHI Yuji, YAMASHITA Yoko, ASHIDA Hitoshi

  日本農芸化学会2017年度大会, Mar. 2017, Japanese, Kyoto, Domestic conference

  Oral presentation


• アシタバカルコンはAMPK経路を介して成熟白色脂肪細胞のベージュ化を促進する

  LiuYiyun, YAMAMOTO Norio, YAMASHITA Yoko, ASHIDA Hitoshi

  日本農芸化学会2017年度大会, Mar. 2017, Japanese, Kyoto, Domestic conference

  Oral presentation


• 大豆の機能性を再発見～食による健康都市づくり～

  YAMASHITA Yoko

  平成２８年度近畿産大豆生産・需要拡大協議会講演会, Feb. 2017, Japanese, Kyoto, Domestic conference

  Public discourse


• 大学における機能性食品に関する研究と健康都市づくり事業について

  YAMASHITA Yoko

  日本食品保健指導士会 関西支部平成２８年度研修会, Feb. 2017, Japanese, Oosaka, Domestic conference

  Public discourse


• クルクミンおよびその誘導体による薬物代謝酵素発現調節機構の解明に関する研究(

  NAKAI Rika, YAMASHITA Yoko, ASHIDA Hitoshi

  日本農芸化学会関西支部第497回講演会, Dec. 2016, Japanese, Kobe, Domestic conference

  Oral presentation


• 脂質・エネルギー代謝と体内時計との関係 〜いつ・何をたべる？〜

  YAMASHITA Yoko

  第24回日本植物油協会植物油栄養懇話会, Nov. 2016, Japanese, Tokyo, Domestic conference

  [Invited]

  Invited oral presentation


• カカオポリフェノールの代謝促進効果：投与タイミングと時計遺伝子

  MITSUHASHI Yuji, YAMASHITA Yoko, ASHIDA Hitoshi

  第21回フードファクター学会学術集会, Nov. 2016, Japanese, Toyama, Domestic conference

  Poster presentation


• Xanthoangelol promotes browning in white adipocyte through activation of AMP-activated protein kinase

  LiuYiyun, YAMAMOTO Norio, YAMASHITA Yoko, ASHIDA Hitoshi

  The 29th Annual and International Meeting of Japanese Association for Animal Cell Technology, Nov. 2016, English, Kobe, International conference

  Poster presentation


• The effect of quercetin and its metabolite isorhamnetin on glucose uptake in myotubes

  JIANG Hao, YOSHIOKA Yasukiyo, YAMASHITA Yoko, ASHIDA Hitoshi

  The 29th Annual and International Meeting of Japanese Association for Animal Cell Technology, Nov. 2016, English, Kobe, International conference

  Oral presentation


• The anti-diabetes effect of quercetin and its metabolite isorhamnetin on glucose uptake in myotubes

  JIANG Hao, YOSHIOKA Yasukiyo, YAMASHITA Yoko, ASHIDA Hitoshi

  JSBBA Kansai (日本農芸化学会関西支部)3rd Student Forum, Nov. 2016, English, Kobe, International conference

  Oral presentation


• Prevention of hyperglycemia and obesity by cacao polyphenols

  ASHIDA Hitoshi, YAMASHITA Yoko

  The 3rd International Conference on Pharma and Food, Nov. 2016, English, Shizuoka, International conference

  [Invited]

  Invited oral presentation


• Piperine induces glucose uptake through AMPK signaling pathway

  MAEDA Ayumi, YAMASHITA Yoko, YOSHIOKA Yasukiyo, AKAGAW Mitsugu, ASHIDA Hitoshi

  The 29th Annual and International Meeting of Japanese Association for Animal Cell Technology, Nov. 2016, English, Kobe, International conference

  Oral presentation


• Luteolin enhanced kaempferol-inhibiting expression of drug-metabolizing enzymes

  NAKAI Rika, YAMASHITA Yoko, ASHIDA Hitoshi

  The 29th Annual and International Meeting of Japanese Association for Animal Cell Technology, Nov. 2016, English, Kobe, International conference

  Poster presentation


• Effect of cacao polyphenols on energy metabolism: Administration timing and clock genes

  MITSUHASHI Yuji, YAMASHITA Yoko, ASHIDA Hitoshi

  JSBBA Kansai (日本農芸化学会関西支部)3rd Student Forum, Nov. 2016, English, Kobe, International conference

  Poster presentation


• 黒大豆種皮由来のプロシアニジンとアントシアニンの肥満・高血糖予防効果とその作用機構

  YAMASHITA Yoko, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi

  第55回 日本栄養・食糧学会近畿支部大会, Oct. 2016, Japanese, Oosaka, Domestic conference

  Oral presentation


• カカオポリフェノール抽出物の投与タイミングと時計遺伝子の関係

  MITSUHASHI Yuji, YAMASHITA Yoko, ASHIDA Hitoshi

  第55回 日本栄養・食糧学会近畿支部大会, Oct. 2016, Japanese, Oosaka, Domestic conference

  Oral presentation


• アシタバカルコンは白色脂肪細胞のベージュ化促進を介して脂質蓄積量を低下させる

  LiuYiyun, YAMAMOTO Norio, YAMASHITA Yoko, ASHIDA Hitoshi

  第55回 日本栄養・食糧学会近畿支部大会, Oct. 2016, Japanese, Oosaka, Domestic conference

  Oral presentation


• Preventive effects of black soybean seed coat extract on fibrosis in the liver of mice

  ASHIDA Hitoshi, RI Xiu, NAMBA FumioToshiya, TODA Toshiya, YAMASHITA Yoko

  The 9th International Conference and Exhibition on Nutraceuticals and Functional Foods 2016 (ISNFF2016), Oct. 2016, English, Florida, International conference

  Oral presentation


• ～食品の生体機能性に関する研究に魅了されて～難消化性ポリフェノールによる糖代謝・脂質代謝促進効果

  YAMASHITA Yoko

  第22回フードサイエンスフォーラム学術集会, Sep. 2016, Japanese, Okayama, Domestic conference

  [Invited]

  Nominated symposium


• 日本ヨモギエタノール抽出物が糖尿病モデルマウスにおよぼす影響

  TSUJI Ai, YAMAMOTO Norio, YAMASHITA Yoko, ASHIDA Hitoshi

  日本農芸化学会平成28年度関西支部大会, Sep. 2016, Japanese, Hikone, Domestic conference

  Oral presentation


• 黒大豆種皮ポリフェノールの抗酸化性を介した炎症性疾患予防効果

  YAMASHITA Yoko, RI Xiu, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi

  日本農芸化学会平成28年度関西支部大会, Sep. 2016, Japanese, Hikone, Domestic conference

  Oral presentation


• ピペリンによる血糖調節作用の機構解明

  MAEDA Ayumi, YOSHIOKA Yasukiyo, YAMASHITA Yoko, AKAGAW Mitsugu, ASHIDA Hitoshi

  第22回フードサイエンスフォーラム学術集会, Sep. 2016, Japanese, Okayama, Domestic conference

  Public symposium


• カテキン誘導体とアルブミンの結合親和性について

  IKEDA Masaki, WAKAGI-UEDA Manabu, HAYASHIBARA Kaori, YAMASHITA Yoko, ASHIDA Hitoshi

  日本農芸化学会平成28年度関西支部大会, Sep. 2016, Japanese, Hikone, Domestic conference

  Oral presentation


• 黒大豆種皮ポリフェノールの抗酸化能を介した疾病予防効果

  YAMASHITA Yoko, LI Xiu, YOSHIOKA Yasukiyo, ASHIDA Hitoshi

  第10回日本ポリフェノール学会, Aug. 2016, Japanese, Tokyo, Domestic conference

  Oral presentation


• 黒大豆加工品中ポリフェノールの体内動態に関する分析

  NAKAMURA Asuka, Liuqing Wang, NAMBA Fumio, TODA Toshiya, ASHIDA Hitoshi, YAMASHITA Yoko

  第10回日本ポリフェノール学会, Aug. 2016, Japanese, Tokyo, Domestic conference

  Poster presentation


• 筋肉細胞の糖取り込みを促進する緑豆タンパク質人工消化物由来ペプチドの単離と特定

  WANG Xin, YAMASHITA Yoko, ASHIDA Hitoshi

  日本食品科学工学会第63回大会, Aug. 2016, Japanese, Nagoya, Domestic conference

  Oral presentation


• ピペリンによる血糖調節作用の機構解明

  MAEDA Ayumi, YOSHIOKA Yasukiyo, YAMASHITA Yoko, AKAGAW Mitsugu, ASHIDA Hitoshi

  日本食品科学工学会第63回大会, Aug. 2016, Japanese, Nagoya, Domestic conference

  Oral presentation


• カテキンの相互作用による血清アルブミンの結合親和性に対する影響

  IKEDA Masaki, WAKAGI-UEDA Manabu, HAYASHIBARA Kaori, YAMASHITA Yoko, ASHIDA Hitoshi

  第10回日本ポリフェノール学会, Aug. 2016, Japanese, Tokyo, Domestic conference

  Poster presentation


• エビデンスに基づいた食による健康寿命の延伸

  YAMASHITA Yoko

  第50回若手栄養学研究者の集いサマーセミナー, Aug. 2016, Japanese, Tokyo, Domestic conference

  Public discourse


• The effect of quercetin and its metabolite isorhamnetin on glucose uptake in myotubes

  JIANG Hao, YOSHIOKA Yasukiyo, YAMASHITA Yoko, ASHIDA Hitoshi

  第10回日本ポリフェノール学会, Aug. 2016, English, Tokyo, Domestic conference

  Poster presentation


• 難消化性ポリフェノールの高血糖・肥満抑制効果に関わる新奇分子ターゲット

  YAMASHITA Yoko, MITSUHASHI Yuji, ASHIDA Hitoshi

  日本農芸化学会関西支部例会, Jul. 2016, Japanese, Oosaka, Domestic conference

  [Invited]

  Invited oral presentation


• ケルセチンとイソラムネチンによる筋肉細胞へのグルコース取り込み促進効果

  ASHIDA Hitoshi, Hao Jiang, YOSHIOKA Yasukiyo, YAMASHITA Yoko

  日本ビタミン学会第68回大会, Jun. 2016, Japanese, Toyama, Domestic conference

  Oral presentation


• ピペリンは骨格筋においてAMPKシグナル経路を介してグルコース取り込みを上昇させる

  MAEDA Ayumi, AKAGAW Mitsugu, YAMASHITA Yoko, ASHIDA Hitoshi

  第70回日本栄養・食糧学会, May 2016, Japanese, Nishinomiya, Domestic conference

  Oral presentation


• クルクミンおよびその誘導体によるAhRを介した薬物代謝酵素の発現調節機構の解明

  NAKAI Rika, FUKUDA Syun, YAMASHITA Yoko, ASHIDA Hitoshi

  第70回日本栄養・食糧学会, May 2016, Japanese, Nishinomiya, Domestic conference

  Oral presentation


• グラブリジン含有組成物のマウスにおける筋肉増強作用機構

  YOSHIOKA Yasukiyo, YAMASHITA Yoko, KISHIDA Hideyuki, NAKAGAWA Kaku, ASHIDA Hitoshi

  第70回日本栄養・食糧学会, May 2016, Japanese, Nishinomiya, Domestic conference

  Oral presentation


• カルコンを高含有するアシタバ抽出物の肥満予防効果

  ASHIDA Hitoshi, ZHANG Tianshun, YAMASHITA Yoko, YAMAMOTO Norio

  第70回日本栄養・食糧学会, May 2016, Japanese, Nishinomiya, Domestic conference

  Oral presentation


• カカオポリフェノール抽出物の投与タイミングと時計遺伝子の発現量および血糖調節作用の関係

  MITSUHASHI Yuji, YAMASHITA Yoko, ASHIDA Hitoshi

  第70回日本栄養・食糧学会, May 2016, Japanese, Nishinomiya, Domestic conference

  Oral presentation


• カカオポリフェノールの時間遺伝子に制御されたエネルギー代謝について

  YAMASHITA Yoko, MITSUHASHI Yuji, ASHIDA Hitoshi

  第70回日本栄養・食糧学会, May 2016, Japanese, Nishinomiya, Domestic conference

  [Invited]

  Invited oral presentation


• 黒大豆種皮抽出物の抗酸化能を介した肝損傷抑制効果について

  YAMASHITA Yoko, RI Xiu, TODA Toshiya, NAMBA Fumio, ASHIDA Hitoshi

  日本農芸化学会2016年度大会, Mar. 2016, Japanese, 札幌, Domestic conference

  Poster presentation


• 筋肉細胞の糖取り込みを促進する緑豆タンパク質人工消化物由来ペプチドの単離と特定

  WANG Xin, YAMASHITA Yoko, ASHIDA Hitoshi

  神戸大学若手フロンティア研究会, Dec. 2015, Japanese, 神戸, Domestic conference

  Poster presentation


• プロシアニジン組成物の投与タイミングと時計遺伝子の発現量および血統調節作用の関係

  MITSUHASHI Yuji, YAMASHITA Yoko, ASHIDA Hitoshi

  第12回日本カテキン学会年次学術大会, Dec. 2015, Japanese, 福岡, Domestic conference

  Poster presentation


• ターメリックに含まれる黄色色素であるクルクミンによるAhRならびにNrf2を介した薬物代謝酵素発現調節機構の解明

  NAKAI Rika, YAMASHITA Yoko, ASHIDA Hitoshi

  第30回 日本香辛料研究会, Dec. 2015, Japanese, 京都, Domestic conference

  Oral presentation


• Procyanidin promotes translocation of glucose transporter 4 in muscle of mice through activation of insulin and AMPK signaling pathways

  YAMASHITA Yoko, ASHIDA Hitoshi

  2015 THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES(PACIFICHEM), Dec. 2015, English, HONOLULU HAWAII, International conference

  Poster presentation


• Prevention of procyanidins and cyaniding 3-glucoside on B(a)P-induced DNA damage through modulating drug-metabolizing enzymes in hepatocytes

  ASHIDA Hitoshi, YAMASHITA Yoko, ZHANG Tianshun

  2015 THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES(PACIFICHEM), Dec. 2015, English, HONOLULU HAWAII, International conference

  [Invited]

  Invited oral presentation


• Piperine enhances glucose uptake through AMPK signaling pathway in skeletal muscle

  MAEDA Ayumi, AKAGAWA Mitsugu, YAMASHITA Yoko, ASHIDA Hitoshi

  2015 THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES(PACIFICHEM), Dec. 2015, English, HONOLULU HAWAII, International conference

  Poster presentation


• Mung bean protein isolate improves glucose tolerance by enhancing glucose transporter 4 translocation and glucose uptake in skeletal muscle

  WANG Xin, YAMASHITA Yoko, TACHIBANA Nobuhiko, WANEZAKI Satoshi, KOHNO Mitsunori, ASHIDA Hitoshi

  2015 THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES(PACIFICHEM), Dec. 2015, English, HONOLULU HAWAII, International conference

  Poster presentation


• GLUT4を介したカテキン代謝物の糖取り込み促進作用

  NAGANO Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

  第12回日本カテキン学会年次学術大会, Dec. 2015, Japanese, 福岡, Domestic conference

  Poster presentation


• 4-Hydroxyderricin and xanthoangerol from Ashitaba(Angelica keisekei) suppress differentiation of preadipocytes to adipocytes via AMPK and MAPK pathways

  ASHIDA Hitoshi, ZHANG Tianshun, YAMASHITA Yoko, YAMAMOTO Norio

  2015 THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES(PACIFICHEM), Dec. 2015, English, HONOLULU HAWAII, International conference

  Poster presentation


• Prevention of obesity by dietary flavonoids

  ASHIDA Hitoshi, YAMASHITA Yoko

  The 6th International Conference on Food Factors(ICOFF2015), Nov. 2015, English, Seoul, International conference

  [Invited]

  Invited oral presentation


• Pleventive effects of cacao polyphenols on hyperglycemia and obesity

  YAMASHITA Yoko, ASHIDA Hitoshi

  The 6th International Conference on Food Factors(ICOFF2015), Nov. 2015, English, Seoul, International conference

  [Invited]

  Invited oral presentation


• Kaempferol modulates expression of drug-metabolizing enzymes through the AhR and Nrf2 pathways

  NAKAI Rika, YAMASHITA Yoko, ASHIDA Hitoshi

  The 6th International Conference on Food Factors(ICOFF2015), Nov. 2015, English, Seoul, International conference

  Poster presentation


• Epigallocatechin gallate and its metabolites promote Traｎｓlocation of glucose transporter 4 in the plasma membrane of muscle cells as a preventive mechanism of hyperglycemia by tea

  ASHIDA Hitoshi, NAGANO Tomoya, YAMASHITA Yoko, UEDA-WAKAGIManabu

  19th International Conference of Functional Foods, Bioactive Compounds and Biomarkers: Longevity and Quality of life, Nov. 2015, English, 神戸, International conference

  [Invited]

  Invited oral presentation


• Effect of catechin metabolites on glucose uptake in myotubes

  NAGANO Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

  19th International Conference of Functional Foods, Bioactive Compounds and Biomarkers: Longevity and Quality of life, Nov. 2015, English, 神戸, International conference

  Poster presentation


• Curcumin modulates expression of drug-metabolizing enzymes through the AhR and Nrf2 pathways in HepG2 cells

  NAKAI Rika, FUNATSU Keiji, YAMASHITA Yoko, ASHIDA Hitoshi

  19th International Conference of Functional Foods, Bioactive Compounds and Biomarkers: Longevity and Quality of life, Nov. 2015, English, 神戸, International conference

  Poster presentation


• Catechin metabolites promote glucose uptake in myotubes

  NAGANO Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

  The 6th International Conference on Food Factors(ICOFF2015), Nov. 2015, English, Seoul, International conference

  Poster presentation


• 施肥条件の異なる黒大豆における抗酸化成分の含量変化について

  WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

  日本栄養・食糧学会 第54回近畿支部大会, Oct. 2015, Japanese, 神戸, Domestic conference

  Oral presentation


• 黒豆種皮含有ポリフェノールは酸化的DNA損傷を抑制する

  YOSHIOKA Yasukiyo, RI Xiu, NAMBA Fumio, TODA Toshiya, YAMASHITA Yoko, ASHIDA Hitoshi

  日本栄養・食糧学会 第54回近畿支部大会, Oct. 2015, Japanese, 神戸, Domestic conference

  Oral presentation


• ダイオキシンが誘導する薬物代謝酵素の発現に対するルテオリンとケンフェロールの抑制効果

  NAKAI Rika, WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

  日本栄養・食糧学会 第54回近畿支部大会, Oct. 2015, Japanese, 神戸, Domestic conference

  Oral presentation


• サフラナールとピペリンは相乗的なグルコース取り込み促進効果を示すのか?

  Maeda Ayumi, AKAGAWA Mitsugu, YAMASHITA Yoko, ASHIDA Hitoshi

  日本栄養・食糧学会 第54回近畿支部大会, Oct. 2015, Japanese, 神戸, Domestic conference

  Oral presentation


• Kaempferol modulates expression of phase Ⅰ and Ⅱdrug-metabolizing enzymes

  ASHIDA Hitoshi, Nakai Rika, YAMASHITA Yoko

  7th International Conference on Polyphenols and Health (ICPH2015), Oct. 2015, English, Tours, International conference

  Poster presentation


• Epigallocatechin gallate and its metabolites promote glucose uptake through translocation of GLUT4 in muscle cells

  NAGANO Tomoya, YAMASHITA Yoko, UEDA-WAKAGIManabu, ASHIDA Hitoshi

  7th International Conference on Polyphenols and Health (ICPH2015), Oct. 2015, English, Tours, International conference

  Poster presentation


• EGCGの筋肉細胞におけるグルコース取り込み促進作用機構の解明

  NAGANO Tomoya, UEDA-WAKAGIManabu, HAYASHIBAR Kaori, YAMASHITA Yoko, ASHIDA Hitoshi

  日本栄養・食糧学会 第54回近畿支部大会, Oct. 2015, Japanese, 神戸, Domestic conference

  Oral presentation


• Chalcones suppress differentiation of preadiopocytes to adipocytes via AMPK and MAPK pathways

  ASHIDA Hitoshi, ZHANG Tianshun, YAMASHITA Yoko, YAMAMOTO Norio

  7th International Conference on Polyphenols and Health (ICPH2015), Oct. 2015, English, Tours, International conference

  Poster presentation


• Development and application of an analysis method for procyanidins by using HPLC coupled with a Efluorescence detector

  WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

  The 8th International Conference and Exhibition for Nutraceuticals and Functional Foods (ISNFF2015), Sep. 2015, English, 無雀, International conference

  Poster presentation


• 黒大豆種皮抽出物による肝損傷抑制効果について

  RI Xiu, YAMASHITA Yoko, ASHIDA Hitoshi

  日本酸化ストレス学会, Jun. 2015, Japanese, 鹿児島, Domestic conference

  Poster presentation


• 卵殻膜由来ペプチドによるによるグルコース取り込み促進効果について

  ASHIDA Hitoshi, TANAKA Yuuki, YAMASHITA Yoko, TAKENAKA Shinji

  日本食品科学工学会, 2015, Japanese, 京都, Domestic conference

  Poster presentation


• β-コングリシニンによる筋肉細胞の分化と萎縮に及ぼす影響について

  KUBOTA Yuusuke, Tachibana Nobuhiko, WANEZAKI Satoshi, KOHNO Mitsunori, YAMASHITA Yoko, ASHIDA Hitoshi

  第28回日本動物細胞工学会2015年度大会(JAACT2015), 2015, Japanese, 仙台, Domestic conference

  Poster presentation


• ポリフェノールの生活習慣病予防効果 －カカオポリフェノールの効果－

  YAMASHITA YOKO

  兵庫工業会特別講演会, 2015, Japanese, 神戸, Domestic conference

  [Invited]

  Nominated symposium


• ピペリンによるグルコース取り込み促進効果の解明とその作用機構解明

  Maeda Ayumi, AKAGAWA Mitsugu, YAMASHITA Yoko, ASHIDA Hitoshi

  日本食品科学工学会, 2015, Japanese, 京都, Domestic conference

  Poster presentation


• ダイオキシンが誘導する消耗性症候群に対するルテオリンの抑制効果について

  ASHIDA Hitoshi, HARADA Kiyonari, MISHIMA Sakie, MITANI Takakazu, YAMASHITA Yoko, MATSUMURA Fumio

  The 2015 Annual Conference of the Japan Society for Bioscience, Biotechnology and Agrochemistry, 2015, Japanese, Domestic conference

  Oral presentation


• クルクミンによるAhRならびにNrf2を介した薬物代謝酵素発現調節機構の解明

  NAKAI Rik, Funatsu Keiji, YAMASHITA Yoko, ASHIDA Hitoshi

  日本食品科学工学会, 2015, Japanese, 京都, Domestic conference

  Poster presentation


• カテキン代謝物による筋肉細胞への糖取り込み促進効果

  NAGANO Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

  日本ポリフェノール学会, 2015, Japanese, 奈良, Domestic conference

  Poster presentation


• カテキン代謝物によるグルコース取り込み促進作用について

  NAGANO Tomoya, YAMASHITA Yoko, ASHIDA Hitoshi

  第28回日本動物細胞工学会2015年度大会(JAACT2015), 2015, Japanese, 仙台, Domestic conference

  Poster presentation


• Oolong, black and pu-erh tea suppresses adiposity in mice via activation of AMP-activated protein kinase

  YAMASHITA Yoko, WANG Liuqing, ASHIDA Hitoshi

  12th Asian Cogress of Nutrition, 2015, English, International conference

  Poster presentation


• Oolong, black and pu-erh tea suppresses adiposity and hyperglycemia in mice via activation of AMP-activated protein kinase

  YAMASHITA Yoko, WANG Liuqing, ASHIDA Hitoshi

  International Symposium on Dietary Antioxidants and Oxidative Stress in Health (DAOSH2015), 2015, English, 淡路, International conference

  Poster presentation


• Kaempferol modulates expression of drug-metabolizing enzymes through the AhR and Nrf2 pathways in HepG2 cells

  NAKAI Rika, KIMURA Yuki, YAMASHITA Yoko, ASHIDA Hitoshi

  12th Asian Cogress of Nutrition, 2015, English, International conference

  Poster presentation


• Caco-2細胞に透過させたカフェインが３T3-L1細胞の分化に与える影響

  HARADA Kiyonari, YAMASHITA Yoko, ASHIDA Hitoshi

  The 2015 Annual Conference of the Japan Society for Bioscience, Biotechnology and Agrochemistry, 2015, Japanese, Domestic conference

  Oral presentation


• Cacao polyphenol promotes energy metabolism under the circadian clock gene expressions

  MITSUHASHI Yuji, YAMASHITA Yoko, NATSUME Midori, ASHIDA Hitoshi

  12th Asian Cogress of Nutrition, 2015, English, International conference

  Poster presentation


• Cacao extract inhibits adipogenesis through suppressing the expression of PPAR gamma and C/EBP alpha

  ASHIDA Hitoshi, MITANI Takakazu, WANG Liuqing, YAMASHITA Yoko

  12th Asian Cogress of Nutrition, 2015, English, International conference

  Oral presentation


• 卵殻膜由来ペプチドによる血糖値上昇抑制効果

  TANAKA Yuuki, TAKENAKA Shinji, YAMASHITA Yoko, ASHIDA Hitoshi

  神戸大学研究基盤センター 若手フロンティア研究会2014, 2014, Japanese, Domestic conference

  Poster presentation


• 発酵茶が脂肪の蓄積に及ぼす効果とその作用機構

  YAMASHITA Yoko, ASHIDA Hitoshi

  The 11th annual meeting of the Japanese Society of Ctechinology, 2014, Japanese, Domestic conference

  Poster presentation


• 発酵茶が脂肪の蓄積に及ぼす効果とその作用機構

  WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

  神戸大学研究基盤センター 若手フロンティア研究会2014, 2014, Japanese, Domestic conference

  Poster presentation


• 脂肪細胞モデルにおいてルテオリンは TCDD が誘導する消耗性症候群を抑制する

  HARADA Kiyonari, MISHIMA Sakie, YAMASHITA Yoko, ASHIDA Hitoshi

  The 19th annual meeting of Japan Society of Food Factors, 2014, Japanese, Domestic conference

  Poster presentation


• 脂肪細胞の分化におけるカフェインとその代謝物の効果

  HARADA Kiyonari, YAMASHITA Yoko, ASHIDA Hitoshi

  The 2014 Annual Conference of the Japan Society for Bioscience, Biotechnology and Agrochemistry in KANSAI, 2014, Japanese, Domestic conference

  Oral presentation


• 黒大豆種皮由来プロシアニジン高含有抽出物が肥満・高血糖に及ぼす効果について

  WATANABE Yuki, YAMASHITA Yoko, NANNBA Fumio, TODA Toshio, ASHIDA Hitoshi

  The 19th annual meeting of Japan Society of Food Factors, 2014, Japanese, Domestic conference

  Poster presentation


• 高感度HPLCを用いたプロシアニジン分析系の構築とその適用

  WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

  The 2014 Annual Conference of the Japan Society for Bioscience, Biotechnology and Agrochemistry in KANSAI, 2014, Japanese, Domestic conference

  Oral presentation


• 紅茶による筋肉細胞へのGLUT4細胞膜移行促進機構の解明

  NAGANO Tomoya, HAYASHIBAR Kaori, YAMASHITA Yoko, ASHIDA Hitoshi

  神戸大学研究基盤センター 若手フロンティア研究会2014, 2014, Japanese, Domestic conference

  Poster presentation


• 蛍光検出器付き HPLC によるプロシアニジン分析法の構築とその適用

  WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

  The 19th annual meeting of Japan Society of Food Factors, 2014, Japanese, Domestic conference

  Poster presentation


• 甘草由来グラブリジン高含有組成物が血糖調節に及ぼす効果

  YAMASHITA Yoko, KISHIDA Hidenobu, NAKAGAWA Kaku, ASHIDA Hitoshi

  The 61st Annual Meeting of the Japanese Society for Food Science and Technology, 2014, Japanese, Domestic conference

  Poster presentation


• 甘草グラブラポリフェノールの主成分であるグラブリジンによる筋肉細胞への糖取り込み促進を介した血糖調節作用

  YAMASHITA Yoko, ASHIDA HITOSHI

  第17回日本補完代替医療学会学術集会, 2014, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• β-コングリシニンの筋肉分化に及ぼす影響について

  KUBOTA Yusuke, TACHIBANA Nobuhiko, WANEZAKI Satoshi, KOUNO Mitsuto, YAMASHITA Yoko, ASHIDA Hitoshi

  The 53th Annual meeting of the Japan Society of Nutrition and Food Science in KINKI, 2014, Japanese, Domestic conference

  Oral presentation


• ルテオリンがAhR-Nrf2経路を介した薬物代謝系酵素発現に与える影響

  KIMURA Yuki, JIANG Songyan, YAMASHITA Yoko, ASHIDA Hitoshi

  The 68th Annual meeting of the Japan Society of Nutrition and Food Science, 2014, Japanese, Domestic conference

  Oral presentation


• メタボリックシンドロームの予防・改善に寄与する機能性成分の網羅的探索法と作用機構解明，ならびにそれを高含有する農作物生産のための環境創造型農業の開発

  YAMASHITA Yoko, ASHIDA Hitoshi, YASUDA Shigeru

  第3回ネイチャー・インダストリー・アワード, 2014, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• プロシアニジンの高血糖・抗肥満予防作用について

  ASHIDA Hitoshi, YAMASHITA Yoko

  第12回日本機能性食品医用学会総会, 2014, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• プロシアニジンのインクレチンならびにエネルギー産生上昇効果

  YAMASHITA Yoko, ASHIDA Hitoshi

  The 68th Annual meeting of the Japan Society of Nutrition and Food Science, 2014, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• ピペリンによるグルコース取り込み促進効果の解明および作用機序の解析

  MAEDA Ayumi, AKAGAWA Mitugu, YAMASHITA Yoko, ASHIDA Hitoshi

  The 29th annual meeting of the japan society for spice and herbResearch, 2014, Japanese, Domestic conference

  Oral presentation


• グラブリジンの筋肉細胞における高血糖抑制効果とその作用機構

  YAMASHITA Yoko, KISHIDA Hidenobu, NAKAGAWA Kaku, ASHIDA Hitoshi

  The 19th annual meeting of Japan Society of Food Factors, 2014, Japanese, Domestic conference

  Oral presentation


• カフェインとその代謝物が脂肪細胞分化に与える影響

  HARADA Kiyonari, YAMASHITA Yoko, ASHIDA Hitoshi

  The 61st Annual Meeting of the Japanese Society for Food Science and Technology, 2014, Japanese, Domestic conference

  Poster presentation


• カカオ豆抽出物による脂肪細胞の分化と脂肪蓄積の抑制効果

  MITANI Takakazu, YAMASHITA Yoko, YAMAMOTO Kazunari, KAWABATA Hiromu, ASHIDA Hitoshi

  The 68th Annual meeting of the Japan Society of Nutrition and Food Science, 2014, Japanese, Domestic conference

  Oral presentation


• カカオリカープロシアニジンの投与時間の違いがエネルギー代謝と時計遺伝子発現におよぼす効果

  光橋 雄史, MITSUHASHI Yuji, YAMASHITA Yoko, NATSUME MIDORI, ASHIDA Hitoshi

  The 19th annual meeting of Japan Society of Food Factors, 2014, Japanese, Domestic conference

  Poster presentation


• Luteolin Modulates Expression of Drug-metabolizing Enzymes through AhR and Nrf2 Pathways in Hepatocytes.

  KIMURA Yuki, ZHANG Tianshun, JIANG Songyan, YAMASHITA Yoko, ASHIDA Hitoshi

  XXVIth International Conference on Polyphenols & 8th Tannin Conference (ICP2014), 2014, English, International conference

  Poster presentation


• Glabridin suppresses hyperglycemia through promotion of glucose uptake accompanied by translocation of glucose transporter 4 in muscle.

  YAMASHITA Yoko, SAWADA Keisuke, KISHIDA Hidenobu, NAKAGAWA Kaku, ASHIDA Hitoshi

  XXVIth International Conference on Polyphenols & 8th Tannin Conference (ICP2014), 2014, English, International conference

  Poster presentation


• Effects of luteolin on TCDD- and tert-butylhydroquinone-induced drug-metabolizing enzynes and nuclear factor-erythroid-2-related factor 2.

  ASHIDA Hitoshi, ZHANG Tianshun, KIMURA Yuki, JIANG Songyan, HARADA Kiyonari, YAMASHITA Yoko

  The 34th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN2014), 2014, English, International conference

  Poster presentation


• Bioavailability of procyanidins in mice.

  WANG Liuqing, YAMASHITA Yoko, ASHIDA Hitoshi

  XXVIth International Conference on Polyphenols & 8th Tannin Conference (ICP2014), 2014, English, International conference

  Poster presentation


• 重合度の異なるカカオ由来プロシアニジンが筋肉細胞でのGLUT4膜移行を介したグルコース取り込みにおよぼす効果

  YAMASHITA Yoko, OKABE Masaaki, NATSUME Midori, ASHIDA Hitoshi

  第60回日本食品科学工学会, 2013, Japanese, 立川, Domestic conference

  Oral presentation


• 黒大豆成分の摂取による血糖値上昇抑制作用とその作用機序解明

  KURIMOTO Yuta, SHIBAYAMA Yuki, INOUE Seiya, SOGA Minoru, TAKIKAWA Masahito, ITO Chiaki, NANBA Fumio, YOSHIDA Tadashi, YAMASHITA Yoko, ASHIDA Hitoshi, TSUDA Takanori

  第60回日本食品科学工学会, 2013, Japanese, 立川, Domestic conference

  Oral presentation


• 黒大豆種皮抽出物による薬物代謝系調節能を介したベンゾピレンの毒性抑制

  KIMURA Yuki, ZHANG Tianshun, JIANG Songyan, HE Chao, YAMASHITA Yoko, ASHIDA Hitoshi

  第67回日本栄養・食糧学会大会, 2013, Japanese, 名古屋, Domestic conference

  Oral presentation


• 黒大豆種皮抽出物によるベンゾピレンの毒性抑制機構の解明

  KIMURA Yuki, ZHANG Tianshun, JIANG Songyan, HE Chao, YAMASHITA Yoko, ASHIDA Hitoshi

  神戸大学若手フロンティア研究会2013, 2013, Japanese, 神戸, Domestic conference

  Poster presentation


• プロシアニジン単回経口投与マウスにおける体内動態に関する研究

  WANG Linqing, YAMASHITA Yoko, ASHIDA Hitoshi

  神戸大学若手フロンティア研究会2013, 2013, Japanese, 神戸, Domestic conference

  Poster presentation


• プロシアニジンのマウスにおける体内動態に関する研究

  WANG Linqing, YAMASHITA Yoko, ASHIDA Hitoshi

  第18回日本フードファクター学会 学術集会(JSoFF2013), 2013, Japanese, 東京, Domestic conference

  Oral presentation


• プロシアニジンのインクレチン効果を介した食後高血糖調節作用

  YAMASHITA Yoko, OKABE Masaaki, NATSUME Midori, ASHIDA Hitoshi

  第67回日本栄養・食糧学会大会, 2013, Japanese, 名古屋, Domestic conference

  Oral presentation


• プロシアニジンによる血糖値上昇抑制効果とその作用機構解明

  YAMASHITA Yoko, OKABE Masaaki, NATSUME Midori, ASHIDA Hitoshi

  日本栄養・食糧学会第52回近畿支部大会, 2013, Japanese, 彦根, Domestic conference

  Oral presentation


• カカオ由来プロシアニジンによるインクレチン効果を介した血糖調整作用

  YAMASHITA Yoko, OKABE Masaaki, NATSUME Midori, ASHIDA Hitoshi

  第18回日本フードファクター学会 学術集会(JSoFF2013), 2013, Japanese, 東京, Domestic conference

  Oral presentation


• カカオポリフェノールの高血糖・肥満抑制効果とその作用機構

  YAMASHITA Yoko, ASHIDA Hitoshi

  第5回食品薬学シンポジウム, 2013, Japanese, 京都, Domestic conference

  Oral presentation


• カカオポリフェノールによる肥満抑制について

  HARADA Kiyonari, ZHANG Tianshun, YAMASHITA Yoko, YAMAMOTO Kazuya, KAWABATA Hiroshi, ASHIDA Hitoshi

  第60回日本食品科学工学会, 2013, Japanese, 立川, Domestic conference

  Oral presentation


• Suppression of adipogenesis-relating factors by fermented tea

  TANAKA Yuki, YAMASHITA Yoko, ASHIDA Hitoshi

  The 5th International conference on O-CHA (Tea) Culture and Science, 2013, English, Shizuoka, Japan, International conference

  Poster presentation


• Prevention mechanism of glucose intolerance and obesity by cacao liquor procyanidin extract

  ASHIDA Hitoshi, YAMASHITA Yoko, OKABE Masaaki, NATSUME Midori

  The IUNS 20th International Congress of Nutrition (ICN2013), 2013, English, Granada, Spain, International conference

  Poster presentation


• Intake of tea prevents postprandial hyperglycemia by promoting GLUT4 translocation in skeletal muscle

  YAMASHITA Yoko, ASHIDA Hitoshi

  The 5th International conference on O-CHA (Tea) Culture and Science, 2013, English, Shizuoka, Japan, International conference

  Poster presentation


• Fermented tea improves glucose intolerance in mice by enhancing translocation of glucose transporter 4 in skeletal muscle

  YAMASHITA Yoko, WANG Lihua, ZHANG Tianshun, NAKAMURA Toshiuki, ASHIDA Hitoshi

  VIth International Conference on Polyphenol and Health, 2013, English, Buenos Aires, Argentina, International conference

  Poster presentation


• Effects of flavonoids on the expression system of drug-metabolizing enzymes

  ASHIDA Hitoshi, ZHANG Tianshun, JIANG Songyan, HE Chao, KIMURA Yuki, YAMASHITA Yoko

  Antioxidants and redox process in health – Bilateral Meeting Japan-Brazil, 2013, English, San Pauro, Brazil, International conference

  [Invited]

  Invited oral presentation


• Black soybean seed coat polyphenols prevent B(a)P-induced DNA damage through modulating drug-metabolizing enzymes in hepatocytes

  ASHIDA Hitoshi, ZHANG Tianshun, JIANG Songyan, HE Chao, Yuki Kimura, YAMASHITA Yoko

  VIth International Conference on Polyphenol and Health, 2013, English, Buenos Aires, Argentina, International conference

  Oral presentation


• Black soybean seed coat extract inhibits genotoxicity induced by benzo[a]pyrene through modulating of drug-metabolizing enzymes

  KIMURA Yuki, ZHANG Tianshun, JIANG Songyan, HE Chao, YAMASHITA Yoko, ASHIDA Hitoshi

  Antioxidants and redox process in health – Bilateral Meeting Japan-Brazil, 2013, English, San Pauro, Brazil, International conference

  Poster presentation


• Black soybean seed coat extract ameliorates hyperglycemia and insulin sensitivity via the activation of AMP-activated protein kinase in diabetic mice

  KURIMOTO Yuta, SHIBAYAMA Yuki, INOUE Seiya, SOGA Minoru, TAKIKAWA Masahito, ITO Chiaki, NANBA Fumio, YOSHIDA Tadashi, YAMASHITA Yoko, ASHIDA Hitoshi, TSUDA Takanori

  7th International Work shop on Anthocyanins, 2013, English, Port, Portogal, International conference

  Oral presentation


• 大豆たん白質由来ペプチドによる筋肉細胞への糖取込み活性促進作用とその作用機序の解明

  SAWADA Keisuke, 山下 陽子YAMASHITA Yoko, ASHIDA Hitoshi

  不二たん白質研究振興財団第15回研究報告会, 2012, Japanese, 仙台, Domestic conference

  Nominated symposium


• 黒大豆成分の血糖値上昇抑制作用とその作用機序

  KURIMOTO Yuta, TAKIKAWA Masahito, SHIBAYAMA Yuki, SOGA Minoru, YAMASHITA Yoko, ASHIDA Hitoshi, TSUDA Takanori

  第66回日本栄養・食糧学会大会, 2012, Japanese, 仙台, Domestic conference

  Oral presentation


• 黒大豆成分の血糖値上昇抑制作用とその作用機構の解明

  KURIMOTO Yuta, TAKIKAWA Masahito, SHIBAYAMA Yuki, SOGA Minoru, YAMASHITA Yoko, ASHIDA Hitoshi, TSUDA Takanori

  日本農芸化学会2012年度大会, 2012, Japanese, 京都, Domestic conference

  Oral presentation


• 酵素処理イソケルシトリンおよびケルセチンがGLUT4細胞膜移行に及ぼす影響

  HORIUCHI Yuko, YAMASHITA Yoko, OGAWA Rui, ASHIDA Hitoshi

  第66回日本栄養・食糧学会大会, 2012, Japanese, 仙台, Domestic conference

  Oral presentation


• 酵素処理イソクエルシトリンおよびケルセチンによる食餌誘導性高血糖の予防効果

  HORIUCHI Yuko, YAMASHITA Yoko, ASHIDA Hitoshi

  日本栄養・食糧学会第51回近畿支部大会, 2012, Japanese, 宝塚, Domestic conference

  Oral presentation


• ロシアニジンの食餌由来高血糖および肥満予防改善効果

  YAMASHITA Yoko, ASHIDA Hitoshi

  第17回日本フードファクター学会学術集会・第9回日本カテキン学会総会合同大会, 2012, Japanese, 静岡, Domestic conference

  Oral presentation


• カカオ由来プロシアニジン高含有組成物の肥満予防効果

  YAMASHITA Yoko, OKABE Masaaki, NATSUME Midori, ASHIDA Hitoshi

  日本農芸化学会2012年度大会, 2012, Japanese, 京都, Domestic conference

  Oral presentation


• 放射化分析による緑茶抽出物を摂取したマウス体毛中の元素分析

  KAWASE Masaya, SAITO Tadashi, NAKANO Yukihiro, YAMASHITA Yoko, IMADA Sayuri, ASHIDA Hitoshi

  京都大学原子炉利用者講演会, 2011, Japanese, Domestic conference

  Oral presentation


• 黒大豆種皮抽出物の肥満抑制作用機構の解明

  YAMASHITA Yoko, KANAMOTO Yuki, NAMBA Fumio, YOSHIDA Tadashi, TSUDA Takanori, NAKAMURA-TSURUTA Sachiko, ASHIDA Hitoshi

  日本農芸化学会2011年度大会, 2011, Japanese, Domestic conference

  Oral presentation


• 酵素処理イソクエルシトリン (EMIQ)および ケルセチンの筋肉細胞へのグルコース取込み促進効果について

  SHIRAO Takeshi, YAMASHITA Yoko, UEDA Manabu, SAWADA Keisuke, ASHIDA Hitoshi

  食品科学工学会第58回大会, 2011, Japanese, Domestic conference

  Oral presentation


• カカオポリフェノールは筋肉細胞でのGLUT4膜移行の促進を介して食後高血糖を低下させる

  YAMASHITA Yoko, UEDA Manabu, OGAWA Rui, NATSUME Midori, ASHIDA Hitoshi

  第65回日本栄養・食糧学大会, 2011, Japanese, Domestic conference

  Oral presentation


• エピカテキン重合体であるプロシアニジンを高含有する組成物の肥満予防効果

  YAMASHITA Yoko, ASHIDA Hitoshi

  第8回日本カテキン学会, 2011, Japanese, Domestic conference

  Oral presentation


• Quercetin and enzymatically modified isoquercitrin stimulate glucose uptake activity in skeletal muscle

  ASHIDA Hitoshi, YAMASHITA Yoko, SHIRAO Takeshi, SAWADA Keisuke, UEDA Manabu

  5th International conference on Polyphenols and Health, 2011, English, International conference

  Poster presentation


• Polyphenol-rich black soybean seed coat extract prevents hyperglycemia, insulin resistance and obesity in high-fat diet-fed C57BL/6J mice

  YAMASHITA Yoko, TSUDA Takanori, YOSHIDA Tadashi, TODA Toshiya, ASHIDA Hitoshi

  XI Asian Congress of Nutrition (ACN2011), 2011, English, International conference

  Poster presentation


• Effect of green tea extract on elemental contents in mice liver

  KAWASE Masaya, YAMASHITA Yoko, IMADA Sayuri, ASHIDA Hitoshi

  5th International conference on Polyphenols and Health, 2011, English, International conference

  Poster presentation


• Cacao liquor procyanidin extract suppresses hyperglycemia through promotion of glucose uptake accompanied by translocation of glucose transporter 4 in muscle

  YAMASHITA Yoko, NATSUME Midori, ASHIDA Hitoshi

  2011 International Conference on Food Factors (ICoFF2011), 2011, English, International conference

  [Invited]

  Invited oral presentation


• Cacao liquor procyanidinextract prevents hyperglycemia in mice

  ASHIDA Hitoshi, YAMASHITA Yoko, NATSUME Midori

  5th International conference on Polyphenols and Health, 2011, English, International conference

  Oral presentation


• Black soybean polyphenol prevents hyperglycemia, insulin resistance and obesity

  ASHIDA Hitoshi, YAMASHITA Yoko, TSUDA Takanori, YOSHIDA Tadashi, TODA Toshiya

  International Society for Nutraceuticals &Functional Foods, 2011 Annual conference (ISNFF2011), 2011, English, International conference

  [Invited]

  Invited oral presentation


• カカオポリフェノール抽出物の筋肉細胞へのグルコース取り込み促進効果について

  YAMASHITA Yoko, UEDA Manabu, NATSUME Midori, ASHIDA Hitoshi

  日本食品科学工学会第57回大会, 2010, Japanese, Domestic conference

  Oral presentation


• Effects of enzymatically synthesized glycogen on lipid methabolism in vivo

  YAMASHITA Yoko, OGAWA Rui, NAKAMURA-TSURUTA Sachiko, FURUYASHIKI Takashi, TAKATA Hiroki, ASHIDA Hitoshi

  PACIFICHEM2010(環太平洋国際化学会議), 2010, English, Domestic conference

  Poster presentation


• Cacao polyphenol extract promotes glucose uptake in muscle cells

  YAMASHITA Yoko, UEDA Manabu, NATSUME Midori, ASHIDA Hitoshi

  The 23rd Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT 2010), 2010, English, International conference

  Poster presentation

• 脳での摂食リズム調節を介した肥満予防に寄与するポリフェノールに関する研究

  山下 陽子

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, Apr. 2024 - Mar. 2027


• 血管機能の向上に寄与する食品成分の探索とその作用機序解明

  公益財団法人 飯島藤十郎記念食品科学振興財団, 2021年度学術研究助成金, Apr. 2022 - Mar. 2024, Principal investigator


• 糖尿病性認知症に寄与する食品成分の時空間的制御機構の解明

  山下 陽子

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Kobe University, Apr. 2020 - Mar. 2023

  本年度は、食餌誘導性肥満モデルマウスにおいて、クロダイズ種皮抽出物が脳機能や肥満・高血糖に及ぼす影響を検討した。①クロダイズ種皮抽出物 (BE) を高脂肪食 (HFD) に混餌した場合に、HFD摂取による食リズムの乱れを是正し、脳での作用が重要な役割を果たしていることを明らかにした。。またその効果にシアニジン 3-グルコシド (C3G) が寄与している可能性を明らかにした。また、②BEの強制単回経口投与は、迷走神経の活性化を介して摂食量を低下させることも明らかにした。①当初の仮説で、BE中のプロシアニジンが、消化管ホルモン分泌を介し、脳機能を是正するのではないかと仮説を立てて研究を行った。HFDの摂取は、視床下部弓状核 (ARC) に炎症をもたしたが、BEの４週間摂取は、このARCでの炎症を抑制し、摂食行動の異常を改善し、肥満も抑制した。現在、これが認知機能に及ぼす影響を検討するため、４８週間投与したマウスを継続して飼育中である。次に、BE中の有効成分を同定するため、単量体フラバン 3-オール、重合体プロシアニジン、およびC3Gの3種類に分画し、HFDへ混餌した結果、C3Gが有効成分であることを明らかにした。②BEの単回強制経口投与における脳機能に与える効果についても検証した。その結果、BEは１回の投与で、消化管からの情報が求心性迷走神経を介して脳に伝達され、摂食を抑制する効果を発揮することがわかった。これらが、認知機能や脳での炎症にもたらす作用機構の詳細も検討し、消化管ホルモンのGLP－１が作用機構の一端を担う可能性を示唆する知見を得た。以上のことより、今年度は、本課題解決で基盤となる、糖尿病性高血糖が認知機能の低下に、BEが効果的に作用することを発見したとともに、今後も重要となる技術や実験手法を独自で確立することができた。


• 油脂の摂取タイミングが体内時計の乱れと肥満に及ぼす影響とその作用機構解明

  公益財団法人 東洋食品研究所, Apr. 2021 - Mar. 2022, Principal investigator


• 抗高血糖効果を持つ食品成分の体内時計の影響とその作用機構解明

  山下 陽子

  公益社団法人日本栄養・食糧学会, 日本栄養食糧学会基金, Apr. 2020 - Mar. 2021


• ポリフェノール・パラドックスの解明

  芦田 均

  科学研究費補助金／基盤研究(A), Apr. 2017 - Mar. 2021

  Competitive research funding


• 難吸収性ポリフェノールにおける時間栄養学的観点からの腸脳相関作用の解明

  山下 陽子

  学術研究助成基金助成金／若手研究(B), Apr. 2017 - Mar. 2020, Principal investigator

  Competitive research funding


• ひょうご健康都市推進協議会 黒大豆

  山下 陽子

  農林水産省, 健康な食生活を支える地域・産業つくり推進事業：機能性農産物等の食による健康都市づくり支援事業(, 神戸大学, Apr. 2016 - Mar. 2019, Principal investigator


• 食料廃棄物を用いた有機資材による高付加価値黒大豆生産のための環境創造型農業の構築

  芦田 均

  科学研究費補助金／基盤研究(B), Apr. 2015 - Mar. 2018

  Competitive research funding


• 筋肉細胞へ糖取り込みを促進する食品成分は運動能力を高めることができるのか？

  芦田 均

  学術研究助成基金助成金／挑戦的萌芽研究, Apr. 2015 - Mar. 2017

  Competitive research funding


• 重合ポリフェノールの消化管ホルモンを介した代謝調節機構に関する研究

  山下 陽子

  学術研究助成基金助成金／若手研究(B), Apr. 2014 - Mar. 2017, Principal investigator

  Competitive research funding


• シカ肉の生体機能解明と高品質・高機能なシカ肉商品の創製

  山下 陽子

  兵庫県, 「農」イノベーションひょうご研究開発プロジェクト支援事業, 2017, Principal investigator

  Competitive research funding


• シカ肉の新たな機能性創出のための研究

  山下 陽子

  兵庫県, 「農」イノベーションひょうご研究開発プロジェクト支援事業, 2015, Principal investigator

  Competitive research funding