Research activity information

• Mar. 2006 日本育種学会, 平成17年度日本育種学会奨励賞, 遺伝子導入によるアブラナ科自家不和合性の制御に関する研究

  Takeshi TAKASAKI

  Japan society


• Apr. 2002 日経BP社, 汎用性の高いハイブリット種子作製技術, 2001年(第11回)日経BP技術賞大賞

  HINATA K, TAKASAKI T, HATAKEYAMA K, SUZUKI G, WATANABE M, ISOGAI A

  Publisher

• Typing and distribution of the SFBB alleles encoded by four S-haplotypes of European pear (Pyrus communis L.)

  K. Tadokoro, M. Kobayashi, R. Fujimoto, T. Takasaki-Yasuda

  Corresponding, International Society for Horticultural Science (ISHS), Sep. 2024, Acta Horticulturae, (1404) (1404), 1051 - 1058

  [Refereed]

  Scientific journal


• A GRAS-Di system linkage map in Chinese cabbage

  H. Mehraj, M.A. Akter, T. Takasaki-Yasuda, E.S. Dennis, R. Fujimoto

  International Society for Horticultural Science (ISHS), Sep. 2024, Acta Horticulturae, (1404) (1404), 323 - 330

  [Refereed]

  Scientific journal


• The role of salicylic acid responsive genes in disease resistance in Brassica rapa vegetable

  M.A. Akter, N. Miyaji, M. Shimizu, T. Takasaki-Yasuda, E.S. Dennis, R. Fujimoto

  International Society for Horticultural Science (ISHS), Mar. 2023, Acta Horticulturae, (1362) (1362), 305 - 312

  [Refereed]

  Scientific journal


• Transcriptional Association between mRNAs and Their Paired Natural Antisense Transcripts Following Fusarium oxysporum Inoculation in Brassica rapa L.

  Mst. Arjina Akter, Hasan Mehraj, Naomi Miyaji, Satoshi Takahashi, Takeshi Takasaki-Yasuda, Motoaki Seki, Elizabeth S. Dennis, Ryo Fujimoto, Kenji Osabe

  Long noncoding RNAs (lncRNAs) play important roles in abiotic and biotic stress responses; however, studies on the mechanism of regulation of lncRNA expression are limited in plants. The present study examined the relationship between lncRNA expression level and two active histone modifications (H3K4me3 and H3K36me3) in Brassica rapa. Both histone marks were enriched in the chromatin regions encoding lncRNAs, especially around the transcription start site. The transcription level of long intergenic noncoding RNAs was positively associated with the level of H3K4me3 and H3K36me3, while this association was not observed in natural antisense RNAs (NATs) and intronic noncoding RNAs. As coordinate expression of mRNAs and paired NATs under biotic stress treatment has been identified, the transcriptional relationship between mRNAs and their paired NATs following Fusarium oxysporum f. sp. conglutinans (Foc) inoculation was examined. A positive association of expression levels between mRNAs and their paired NATs following Foc inoculation was observed. This association held for several defense-response-related genes and their NAT pairs. These results suggest that coordinate expression of mRNAs and paired NATs plays a role in the defense response against Foc.

  MDPI AG, Dec. 2021, Horticulturae, 8(1) (1), 17 - 17

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The transcriptional response to salicylic acid plays a role in Fusarium yellows resistance in Brassica rapa L.

  Naomi Miyaji, Motoki Shimizu, Takeshi Takasaki-Yasuda, Elizabeth S Dennis, Ryo Fujimoto

  KEY MESSAGE: Fusarium yellows resistant and susceptible lines in Brassica rapa showed different salicylic acid responses; the resistant line showed a similar response to previous reports, but the susceptible line differed. Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans (Foc) is an important disease. Previous studies showed that genes related to salicylic acid (SA) response were more highly induced following Foc infection in Brassica rapa Fusarium yellows resistant lines than susceptible lines. However, SA-induced genes have not been identified at the whole genome level and it was unclear whether they were up-regulated by Foc inoculation. Transcriptome analysis with and without SA treatment in the B. rapa Fusarium yellows susceptible line 'Misugi' and the resistant line 'Nanane' was performed to obtain insights into the relationship between SA sensitivity/response and Fusarium yellows resistance. 'Nanane's up-regulated genes were related to SA response and down-regulated genes were related to jasmonic acid (JA) or ethylene (ET) response, but differentially expressed genes in 'Misugi' were not. This result suggests that Fusarium yellows resistant and susceptible lines have a different SA response and that an antagonistic transcription between SA and JA/ET responses was found only in a Fusarium yellows resistant line. SA-responsive genes were induced by Foc inoculation in Fusarium yellows resistant (RJKB-T23) and susceptible lines (RJKB-T24). By contrast, 39 SA-induced genes specific to RJKB-T23 might function in the defense response to Foc. In this study, SA-induced genes were identified at the whole genome level, and the possibility, the defense response to Foc observed in a resistant line could be mediated by SA-induced genes, is suggested. These results will be useful for future research concerning the SA importance in Foc or other diseases resistance in B. rapa.

  Apr. 2021, Plant cell reports, 40(4) (4), 605 - 619, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Gene Expression Analysis in Response to Vernalization in Chinese Cabbage (Brassica rapa L.)

  Ayasha Akter, Junji Miyazaki, Daniel J. Shea, Namiko Nishida, Satoko Takada, Naomi Miyaji, Hasan Mehraj, Motoki Shimizu, Md. Asad-ud Doullah, Takeshi Takasaki-Yasuda, Keiichi Okazaki, Ryo Fujimoto

  Japanese Society for Horticultural Science, 2020, The Horticulture Journal, 89(3) (3), 268 - 277

  [Refereed]

  Scientific journal


• The role of FRIGIDA and FLOWERING LOCUS C genes in flowering time of Brassica rapa leafy vegetables.

  Satoko Takada, Ayasha Akter, Etsuko Itabashi, Namiko Nishida, Daniel J Shea, Naomi Miyaji, Hasan Mehraj, Kenji Osabe, Motoki Shimizu, Takeshi Takasaki-Yasuda, Tomohiro Kakizaki, Keiichi Okazaki, Elizabeth S Dennis, Ryo Fujimoto

  There is a wide variation of flowering time among lines of Brassica rapa L. Most B. rapa leafy (Chinese cabbage etc.) or root (turnip) vegetables require prolonged cold exposure for flowering, known as vernalization. Premature bolting caused by low temperature leads to a reduction in the yield/quality of these B. rapa vegetables. Therefore, high bolting resistance is an important breeding trait, and understanding the molecular mechanism of vernalization is necessary to achieve this goal. In this study, we demonstrated that BrFRIb functions as an activator of BrFLC in B. rapa. We showed a positive correlation between the steady state expression levels of the sum of the BrFLC paralogs and the days to flowering after four weeks of cold treatment, suggesting that this is an indicator of the vernalization requirement. We indicate that BrFLCs are repressed by the accumulation of H3K27me3 and that the spreading of H3K27me3 promotes stable FLC repression. However, there was no clear relationship between the level of H3K27me3 in the BrFLC and the vernalization requirement. We also showed that if there was a high vernalization requirement, the rate of repression of BrFLC1 expression following prolonged cold treatments was lower.

  Sep. 2019, Scientific reports, 9(1) (1), 13843 - 13843, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Genome-wide characterization of DNA methylation, small RNA expression, and histone H3 lysine nine di-methylation in Brassica rapa L.

  Satoshi Takahashi, Kenji Osabe, Naoki Fukushima, Shohei Takuno, Naomi Miyaji, Motoki Shimizu, Takeshi Takasaki-Yasuda, Yutaka Suzuki, Elizabeth S Dennis, Motoaki Seki, Ryo Fujimoto

  Epigenetic gene regulation is crucial to plant life and can involve dynamic interactions between various histone modifications, DNA methylation, and small RNAs. Detailed analysis of epigenome information is anticipated to reveal how the DNA sequence of the genome is translated into the plant's phenotype. The aim of this study was to map the DNA methylation state at the whole genome level and to clarify the relationship between DNA methylation and transcription, small RNA expression, and histone H3 lysine 9 di-methylation (H3K9me2) in Brassica rapa. We performed whole genome bisulfite sequencing, small RNA sequencing, and chromatin immunoprecipitation sequencing using H3K9me2 antibody in a Chinese cabbage inbred line, RJKB-T24, and examined the impact of epigenetic states on transcription. Cytosine methylation in DNA was analysed in different sequence contexts (CG, CHG, and CHH) (where H could be A, C, or T) and position (promoter, exon, intron, terminator, interspersed repeat regions), and the H3K9me2 and 24 nucleotide small interfering RNAs (24 nt-siRNA) were overlaid onto the B. rapa reference genome. The epigenome was compared with that of Arabidopsis thaliana and the relationship between the position of DNA methylation and gene expression, and the involvement of 24 nt siRNAs and H3K9me2 are discussed.

  Oct. 2018, DNA research : an international journal for rapid publication of reports on genes and genomes, 25(5) (5), 511 - 520, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Characterization of FLOWERING LOCUS C genes in leafy Brassica rapa vegetables

  FUJIMOTO RYO, Ayasha Akter, Namiko Nishida, Takeshi Takasaki-Yasuda, Elizabeth S Dennis

  Sep. 2018, AusCanola 2018 Proceedings E-book, 71 - 75, English

  [Invited]

  International conference proceedings


• Identification of DNA methylated regions by using methylated DNA immunoprecipitation sequencing in Brassica rapa

  Satoshi Takahashi, Naoki Fukushima, Kenji Osabe, Etsuko Itabashi, Motoki Shimizu, Naomi Miyaji, Takeshi Takasaki-Yasuda, Yutaka Suzuki, Motoaki Seki, Ryo Fujimoto

  CSIRO, 2018, Crop and Pasture Science, 69(1) (1), 107 - 120, English

  [Refereed]

  International conference proceedings

  Link to Kobe Univ. Repository (Kernel)


• Complex dominance hierarchy controlled by polymorphism of small RNAs and their targets

  Yasuda, S, Wada, Y, Kakizaki, T, Tarutani, Y, Miura-Uno, E, Murase, K, Fujii, S, Hioki, T, Shimoda, T, Takada, Y, Shiba, H, Takasaki-Yasuda, T, Suzuki, G, Watanabe, M, Takayama, S

  In diploid organisms, phenotypic traits are often biased by effects known as Mendelian dominant-recessive interactions between inherited alleles. Phenotypic expression of SP11 alleles, which encodes the male determinants of self-incompatibility in Brassica rapa, is governed by a complex dominance hierarchy1-3. Here, we show that a single polymorphic 24 nucleotide small RNA, named SP11 methylation inducer 2 (Smi2), controls the linear dominance hierarchy of the four SP11 alleles (S44 > S60 > S40 > S29). In all dominant-recessive interactions, small RNA variants derived from the linked region of dominant SP11 alleles exhibited high sequence similarity to the promoter regions of recessive SP11 alleles and acted in trans to epigenetically silence their expression. Together with our previous study4, we propose a new model: sequence similarity between polymorphic small RNAs and their target regulates mono-allelic gene expression, which explains the entire five-phased linear dominance hierarchy of the SP11 phenotypic expression in Brassica.

  Dec. 2016, Nature Plants, 3, 1 - 5, English, International magazine

  [Refereed]

  Scientific journal


• The epigenetic regulation of FLC expression by vernalization in Brassica rapa L

  Satoko Takada, Etsuko Itabashi, Naomi Miyaji, Takeshi Takasaki-Yasuda, Kenji Osabe, Elizabeth S. Dennis, FUJIMOTO RYO

  Oct. 2016, Brassica 2016 Abstract Book, 220 - 225, English

  International conference proceedings


• Role of DNA methylation in hybrid vigor in Arabidopsis thaliana

  Takahiro Kawanabe, Sonoko Ishikura, Naomi Miyaji, Taku Sasaki, Li Min Wu, Etsuko Itabashi, Satoko Takada, Motoki Shimizu, Takeshi Takasaki-Yasuda, Kenji Osabe, W. James Peacock, Elizabeth S. Dennis, Ryo Fujimoto

  Oct. 2016, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 113(43) (43), E6704 - E6711, English

  [Refereed]

  Scientific journal


• Identification of the Fusarium yellows resistance genes; its application for marker-assisted selection in Brassica rapa

  Naomi Miyaji, Motoki Shimizu, Satoko Takada, Takeshi Takasaki-Yasuda, Makoto Kaji, Kenji Osabe, Elizabeth S. Dennis, Keiichi Okazaki, FUJIMOTO RYO

  Oct. 2016, Brassica 2016 Abstract Book, 185 - 190, English

  International conference proceedings


• Genome-wide analysis of DNA methylation in Chinese cabbage

  Naoki Fukushima, Satoshi Takahashi, Etsuko Itabashi, Motoki Shimizu, Kenji Osabe, Takeshi Yasuda, Yutaka Suzuki, Motoaki Seki, Elizabeth S. Dennis, FUJIMOTO RYO

  Oct. 2016, Brassica 2016 Abstract Book, 172 - 177, English

  International conference proceedings


• Ultrastructural Changes in the stigma and upper style during floral development of Japanese pear.

  Li Lily, Okamura Ayaha, Nakanishi Tetsu, Fujimoto Ryo, TAKASAKI-YASUDA TAKESHI

  Corresponding, 医学生物学電子顕微鏡技術学会, Mar. 2016, J. Electr. Microsc.Technol. Med. Biol., 29(2) (2), 1 - 7, English

  [Refereed]

  Scientific journal


• Cloning an S-RNase allele, including the longest intron, from cultivars of European pear (Pyrus communis L.)

  T. Takasaki-Yasuda, N. Nomura, Y. Moriya-Tanaka, K. Okada, H. Iwanami, H. Bessho

  Headley Brothers Ltd, 2013, Journal of Horticultural Science and Biotechnology, 88(4) (4), 427 - 432, English

  [Refereed]

  Scientific journal


• S-RNase-based genotyping of seven triploid cultivars of European pear (Pyrus communis L.)

  T. Takasaki-Yasuda, N. Nomura, Y. Moriya-Tanaka, K. Okada, H. Iwanami, H. Bessho

  Headley Brothers Ltd, 2013, Journal of Horticultural Science and Biotechnology, 88(6) (6), 751 - 755, English

  [Refereed]

  Scientific journal


• Dual recognition of S (1) and S (4) pistils by S (4) (sm) pollen in self-incompatibility of Japanese pear (Pyrus pyrifolia Nakai)

  Toshihiro Saito, Yoshihiko Sato, Yutaka Sawamura, Moriyuki Shoda, Takeshi Takasaki-Yasuda, Kazuo Kotobuki

  Aug. 2012, TREE GENETICS & GENOMES, 8(4) (4), 689 - 694, English

  [Refereed]

  Scientific journal


• Related polymorphic F-box protein genes between haplotypes clustering in the BAC contig sequences around the S-RNase of Japanese pear

  Kazuma Okada, Nozomi Tonaka, Tomio Taguchi, Takehiko Ichikawa, Yutaka Sawamura, Tetsu Nakanishi, Takeshi Takasaki-Yasuda

  Corresponding, Mar. 2011, JOURNAL OF EXPERIMENTAL BOTANY, 62(6) (6), 1887 - 1902, English

  [Refereed]

  Scientific journal


• Renumbering the S-RNase alleles of European pears (Pyrus communis L.) and cloning the S109-RNase allele.

  Goldway, M, Sanzol, J, Mota, M, Zisovich, A, Stern, R, Sansavini, S, Takasaki-Yasuda, T

  Apr. 2009, Scientia Horticulturae, 119 (4): 417-422., English

  [Refereed]

  Scientific journal


• S-genotype Assignments of Local Cultivars in Japanese Pear 'Senryo', 'Kuroki', and 'Hogyoku'

  Kazuma Okada, Carlos Castillo, Yutaka Sawamura, Tetsu Nakanishi, Takeshi Takasaki-Yasuda

  Corresponding, Jan. 2009, JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, 78(1) (1), 55 - 60, English

  [Refereed]

  Scientific journal


• Characterisation of partial self-compatibility in the European pear cultivar, 'Grand Champion'

  Yuki Moriya, Kazuma Okada, Kentaro Yamamoto, Hiroshi Iwanami, Hideo Bessho, Takeshi Takasaki-Yasuda

  Corresponding, Jan. 2009, JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY, 84(1) (1), 77 - 82, English

  [Refereed]

  Scientific journal


• Deletion of a 236 kb region around S-4-RNase in a stylar-part mutant S-4(sm)-haplotype of Japanese pear

  Kazuma Okada, Nozomi Tonaka, Yuki Moriya, Naoko Norioka, Yutaka Sawamura, Tatsuya Matsumoto, Tetsu Nakanishi, Takeshi Takasaki-Yasuda

  Corresponding, Mar. 2008, PLANT MOLECULAR BIOLOGY, 66(4) (4), 389 - 400, English

  [Refereed]

  Scientific journal


• S-Genotype Assignment of European Pear Cultivars Using S-RNase Specific CAPS Marker

  T. Takasaki-Yasuda, Y. Moriya, T. Nakanishi, H. Iwanami, H. Bessho

  2008, PROCEEDINGS OF THE XTH INTERNATIONAL PEAR SYMPOSIUM, VOLS 1 AND 2, 800: 391-400.(800) (800), 391 - +, English

  [Refereed]

  International conference proceedings


• Selection of Self-Compatible Trees by S-4(sm)-Haplotype Specific Marker in Japanese Pear

  K. Okada, N. Tonaka, T. Takasaki, T. Matsumoto, Y. Sawamura

  2008, PROCEEDINGS OF THE XTH INTERNATIONAL PEAR SYMPOSIUM, VOLS 1 AND 2, 800: 401-408.(800) (800), 401 - +, English

  [Refereed]

  International conference proceedings


• Development of a CAPS marker system for genotyping European pear cultivars harboring 17 S alleles

  Yuki Moriya, Kentaro Yamamoto, Kazuma Okada, Hiroshi Iwanami, Hideo Bessho, Tetsu Nakanishi, Takeshi Takasaki

  Mar. 2007, PLANT CELL REPORTS, 26(3) (3), 345 - 354, English

  [Refereed]

  Scientific journal


• Development of a CAPS marker system for genotyping European pear cultivars harboring 17 alleles

  Takeshi Takasaki

  Feb. 2007, Plant Cell Reports, 26(3): 345-354., English

  [Refereed]

  Scientific journal


• cDNA cloning of nine S alleles and establishment of a PCR-RFLP system for genotyping European pear cultivars

  T Takasaki, Y Moriya, K Okada, K Yamamoto, H Iwanami, H Bessho, T Nakanishi

  May 2006, THEORETICAL AND APPLIED GENETICS, 112(8) (8), 1543 - 1552, English

  [Refereed]

  Scientific journal


• Parthenocarpy and self- and cross-incompatibility in ten European pear cultivars

  Y Moriya, Y Takai, K Okada, D Ito, Y Shiozaki, T Nakanishi, T Takasaki

  Nov. 2005, JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, 74(6) (6), 424 - 430, English

  [Refereed]

  Scientific journal


• Parthenocarpy and Self- and Cross-incompatibility in Ten European Pear Cultivars

  Moriya Yuki, Takai Yoshihiro, Okada Kazuma, Ito Daiyu, Shiozaki Yunosuke, Nakanishi Tetsu, Takasaki Takeshi

  Self- and cross-incompatibility in European pear (<i>Pyrus communis</i> L.), which is estimated by the fruit set and seed formation, is yet unclear. We carried out self-, cross- and non-pollinations with one flower per cluster on 10 European pear cultivars, and estimated their parthenocarpic potential, and self- and cross-incompatibility. Most cultivars exhibited parthenocarpy, which suggests that the fruit set is not a suitable criterion for distinguishing incompatibility from compatibility. However, clear judgment could be provided by using the self-incompatibility (SI) index, (the number of viable seeds per flower obtained from test pollination / the number of viable seeds per flower resulting from compatible cross-pollination) × 100, as a new criterion. Based on this index, 'Grand Champion' has proven to be partially self-compatible, whereas others were classified as self-incompatible. Traits of the seeded fruits, such as weight, size, and soluble solids content, were superior to those of the parthenocarpic fruits. Thus, cross-compatible pollination is necessary for a stable fruit set and production of large, good quality fruits in cultivars with a high parthenocarpic potential. Two cross-incompatible combinations were found between 'Flemish Beauty' and 'Starkrimson', and 'Bartlett' and 'Seigneur d' Espéren', respectively.

  一般社団法人 園芸学会, 2005, Journal of the Japanese Society for Horticultural Science, 74(6) (6), 424 - 430


• Molecular cloning, functional expression and characterization of two serine/threonine-specific protein kinases from Nicotiana tabacum pollen

  K Dissanayake, C Castillo, T Takasaki, T Nakanishi, N Norioka, S Norioka

  Dec. 2004, SEXUAL PLANT REPRODUCTION, 17(4) (4), 165 - 175, English

  Scientific journal


• Reconsideration of S-genotype for a Japanese pear 'Kumoi'

  K Okada, T Takasaki, T Saito, Y Moriya, C Castillo, S Norioka, T Nakanishi

  Nov. 2004, JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, 73(6) (6), 524 - 528, English

  Scientific journal


• Pollination effects of the sweat bee Lasioglossum villosulum trichopse (Hymenoptera : Halictidae) on genic male-sterile lettuce

  M Goubara, T Takasaki

  Feb. 2004, APPLIED ENTOMOLOGY AND ZOOLOGY, 39(1) (1), 163 - 169, English

  Scientific journal


• Sequence of the S-9-RNase cDNA and PCR-RFLP system for discriminating S-1- to S-9-allele in Japanese pear

  T Takasaki, K Okada, C Castillo, Y Moriya, T Saito, Y Sawamura, N Norioka, S Norioka, T Nakanishi

  2004, EUPHYTICA, 135(2) (2), 157 - 167, English

  Scientific journal


• Effect of silver nitrate on shoot regeneration and Agrobacterium - Mediated transformation of turnip (Brassica rapa L. var. rapifera)

  Takeshi Takasaki, Katsunori Hatakeyama, Kokichi Hinata

  Japanese Society for Plant Cell and Molecular Biology, 2004, Plant Biotechnology, 21(3) (3), 225 - 228, English

  Scientific journal


• Flower visitors of lettuce under field and enclosure conditions

  M Goubara, T Takasaki

  Nov. 2003, APPLIED ENTOMOLOGY AND ZOOLOGY, 38(4) (4), 571 - 581, English

  Scientific journal


• Flower visitors of lettuce under field and enclosure conditions.

  GOUBARA M, TAKASAKI T

  Oct. 2003, Applied Entomology and Zoology, 38 (4): 571-581., English

  [Refereed]

  Scientific journal


• An efficient and reproducible in vitro plant regeneration from leaf discs in pear cultivars (Pyrus spp.).

  HENNAYAKA CK, DISSANAYAKE K, MATSUDA N, TAKASAKI T, NAKANISHI T

  Aug. 2003, Plant Biotechnology, 20 (4): 283-289, English

  [Refereed]

  Scientific journal


• Antisense inhibition of a nuclear gene, BrDAD1, in Brassica causes male sterility that is restorable with jasmonic acid treatment

  K Hatakeyama, S Ishiguro, K Okada, T Takasaki, K Hinata

  May 2003, MOLECULAR BREEDING, 11(4) (4), 325 - 336, English

  Scientific journal


• Antisense inhibition of a nuclear gene, BrDAD1, in Brassica causes male sterility that is restorable with jasmonic acid treatment

  HATAKEYAMA K, ISHIGURO S, OKADA K, TAKASAKI T, HINATA K

  Mar. 2003, Molecular Breeding, 11(4) (4), 325 - 336, English

  [Refereed]

  Scientific journal


• An efficient and reproducible in vitro plant regeneration from leaf discs in pear cultivars(Pyrus spp.).

  HENNAYAKE K, DISSANAYAKE K, MATSUDA N, TAKASAKI Takeshi, NAKANISHI Tetsu

  2003, Plant Biotech, 20(4),283-289.(b)(c)(e), English

  Scientific journal


• S-RNase based PCR-RFLP system for S-genotype assignment in Japanese pear

  C. Castillo, T. Nakanishi, T. Ishimizu, T. Takasaki, S. Norioka, T. Saito

  International Society for Horticultural Science, 2002, Acta Horticulturae, 587, 449 - 458, English

  [Refereed]

  International conference proceedings


• The S receptor kinase gene determines dominance relationships in stigma expression of self-incompatibility in Brassica.

  Hatakeyama, K, Takasaki, T, Suzuki, G, Nishio, T, Watanabe, M, Isogai, A, Hinata, K

  ISI 被引用数：25

  Sep. 2001, Plant J., 26, 69 - 76, English

  [Refereed]

  Scientific journal


• Sequence comparison of the 5' flanking regions of Japanese pear ( Pyrus pyrifolia ) S-RNases associated with gametophytic self-incompatibility

  Naoko Norioka, Hironori Katayama, Tohru Matsuki, Takeshi Ishimizu, Takeshi Takasaki, Tetsu Nakanishi, S. Norioka

  Springer Science and Business Media {LLC}, May 2001, Sexual Plant Reproduction, 13(5) (5), 289 - 291

  [Refereed]

  Scientific journal


• Sequence comparison of the 5 ' flanking regions of Japanese pear (Pyrus pyrifolia) S-RNases associated with gametophytic self-incompatibility

  N Norioka, H Katayama, T Matsuki, T Ishimizu, T Takasaki, T Nakanishi, S Norioka

  May 2001, SEXUAL PLANT REPRODUCTION, 13(5) (5), 289 - 291, English

  [Refereed]

  Scientific journal


• Homology-dependent suppression of stigma phenotype by an antisense S-locus glycoprotein (SLG) gene in Brassica rapa L.

  Takasaki, T, Hatakeyama, K, Watanabe, M, Toriyama, K, Hinata, K

  ISI 被引用数：1

  Apr. 2001, Breed. Sci., 51, 89 - 94, English

  [Refereed]

  Scientific journal


• Expression of a fusion protein of Pyrus pyrifolia S-Rnase with glutathione-S-transferase in E. coli

  Niranjali Gamage, Takanori Matsuura, Naoko Norioka, Yumi Yoshimura, Takeshi Takasaki, Tetsu Nakanishi, Shigemi Norioka

  Sep. 2000, Biotechnology Letters, 22(17) (17), 1413 - 1417, English

  [Refereed]

  Scientific journal


• Highly divergent sequences of the pollen self-incompatibility (S) gene in class-I S haplotypes of Brassica campestris (syn. rapa) L.

  Masao Watanabe, Akiko Ito, Yoshinobu Takada, Chie Ninomiya, Tomohiro Kakizaki, Yoshihito Takahata, Katsunori Hatakeyama, Kokichi Hinata, Go Suzuki, Takeshi Takasaki, Yoko Satta, Hiroshi Shiba, Seiji Takayama, Akira Isogai

  May 2000, FEBS Letters, 473(2) (2), 139 - 144

  Scientific journal


• The S receptor kinase determines self-incompatibility in Brassica stigma

  Takeshi Takasaki, Katsunori Hatakeyama, Go Suzuki, Masao Watanabe, Akira Isogai, Kokichi Hinata

  Feb. 2000, Nature, 403(6772) (6772), 913 - 916, English

  [Refereed]

  Scientific journal


• Introduction of SLG (S-locus glycoprotein) alters the phenotype of endogenous S-haplotype, but confers no new S-haplotype specificity in Brassica rapa L.

  Takasaki, T, Hatakeyama, K, Watanabe, M, Toriyama, K, Isogai, A, Hinata, K

  ISI 被引用数：25

  Aug. 1999, Plant Mol. Biol., 40, 659 - 668, English

  [Refereed]

  Scientific journal


• High sequence similarity between SLG and the receptor domain of SRK is not necessarily involved in higher dominance relationships in stigma in self-incompatible Brassica rapa L.

  Hatakeyama, K, Takasaki, T, Watanabe, M, Hinata, K

  ISI 被引用数：9

  Oct. 1998, Sex. Plant Reprod., 11, 292 - 294, English

  [Refereed]

  Scientific journal


• Molecular characterization of S locus genes, SLG and SRK, in a pollen-recessive self-incompatibility haplotype of Brassica rapa L.

  Hatakeyama, K, Takasaki, T, Watanabe, M, Hinata, K

  ISI 被引用数：30

  Sep. 1998, Genetics, 149, 1587 - 1597, English

  [Refereed]

  Scientific journal


• Dominance relationships between S-alleles in self-incompatible Brassica campestris L.

  Hatakeyama, K, Watanabe, M, Takasaki, T, Ojima, K, Hinata, K

  ISI 被引用数：39

  Mar. 1998, Heredity, 80, 241 - 247, English

  [Refereed]

  Scientific journal


• Factors Influencing Agrobacterium -mediated Transformation of Brassica rapa L

  Takeshi Takasaki, Katsunori Hatakeyama, Kunihiko Ojima, Masao Watanabe, Kinya Toriyama, Kokichi Hinata

  Japanese Society of Breeding, 1997, Breeding Science, 47(2) (2), 127 - 134, English

  Scientific journal

• Brassica rapaの極晩抽性系統の選抜とFLCの春化応答性の調査

  矢野秀汰, 清水元樹, 安田剛志, 藤本龍

  2024, 園芸学研究 別冊, 23(1) (1)


• 萎黄病抵抗性・感受性ハクサイ系統における萎黄病菌感染時の免疫応答

  宮路直実, 清水元樹, 加治誠, 安田剛志, 岡崎桂一, 藤本龍

  2019, 日本植物病理学会大会プログラム・講演要旨予稿集, 2019


• Brassica rapa L.における春化によるFLOWERING LOCUS Cのエピジェネティックな転写制御について

  高田紗都子, 板橋悦子, 宮路直実, 清水元樹, 安田剛志, 藤本龍

  2016, 園芸学研究 別冊, 15(2) (2)


• Whole genome bisulfite sequencingによるハクサイのDNAメチル化領域の探索

  福島尚樹, 高橋聡史, 板橋悦子, 清水元樹, 安田剛志, 鈴木穣, 関原明, 藤本龍

  2016, 園芸学研究 別冊, 15(2) (2)


• ハクサイにおけるメチローム解析

  福島尚樹, 高橋聡史, 板橋悦子, 清水元樹, 安田剛志, 鈴木穣, 関原明, 藤本龍

  2016, 育種学研究, 18


• 萎黄病菌を接種したときの抵抗性と感受性ハクサイ系統のトランスクリプトームプロファイリング

  宮路直実, 清水元樹, 高田紗都子, 加治誠, 安田剛志, 岡崎桂一, 藤本龍

  2016, 育種学研究, 18


• ニホンナシ在来品種‘千両’,‘黒木’と‘宝玉’のS遺伝子型同定

  岡田和馬, CASTILLO Carlos, 澤村豊, 中西テツ, 安田(高崎)剛志

  27 Sep. 2008, 園芸学研究 別冊, 7(2) (2), 103, Japanese


• 遺伝子導入によるアブラナ科自家不和合性の制御

  Takeshi Takasaki

  Japanese Society of Breeding, Sep. 2006, 育種学研究, 8(3)127-134.(3) (3), 127 - 134, Japanese

  Introduction scientific journal


• Stigma- and pollen-S genes determine self-incompatibility in Brassica

  TAKASAKI Takeshi

  Kobe University, 25 Feb. 2002, The science reports of Faculty of Agriculture, Kobe University, 26, 43 - 46, Japanese


• Cloning of the S8-RNase (S8-allele) of Japanese pear (Pyrus pyrifolia nakai)

  Carlos Castillo, Takeshi Takasaki, Toshihiro Saito, Shigemi Norioka, Tetsu Nakanishi

  Japanese Society for Plant Cell and Molecular Biology, 2002, Plant Biotechnology, 19(1) (1), 1 - 6, English


• Reconsideration of S-genotype assignments, and discovery of a new allele based on S-RNase PCR-RFLPs in Japanese pear cultivars

  C Castillo, T Takasaki, T Saito, Y Yoshimura, S Norioka, T Nakanishi

  Mar. 2001, BREEDING SCIENCE, 51(1) (1), 5 - 11, English

• 果樹園芸学 第5章 ナシ

  TAKASAKI-YASUDA TAKESHI, 田村文男

  Joint work, 文永堂, Mar. 2015, Japanese, 第5章 ナシ

  Textbook


• 果樹園芸学 第10章果樹園芸学の発展に多大な貢献をした人々-前田正名と福羽逸人によるオリーブの導入と普及-

  TAKASAKI-YASUDA TAKESHI, 中西テツ

  Joint work, 文永堂, Mar. 2015, Japanese

  Textbook

• フラグメント解析によるナシ自家不和合性遺伝子型判定マーカーの開発

  西村遼太郎, 西尾総悟, 藤本龍, 安田(高崎)剛志

  園芸学会令和6年度秋季大会, Nov. 2024

  Oral presentation


• 相同性が高いS-RNaseをもつセイヨウナシとニホンナシの交雑不和合;和合性

  田所晃汰, 安田(高崎)剛志

  令和６年度園芸学会近畿支部滋賀大会, Aug. 2024

  Poster presentation


• Typing and distribution of the SFBB alleles encoded by four S-haplotypes of European pear (Pyrus communis L.)

  K. Tadokoro a, M. Kobayashi, R. Fujimoto, T. Takasaki-Yasuda

  The 4th Asian Horticultural Congress (AHC2023), Aug. 2023

  Poster presentation


• ニホンナシ S2, S3, S4 ハプロタイプのゲノム構造解析と SFBB の探索

  西村遼太郎, 溝口桃香, 張 競月, 藤本龍, 安田(高崎)剛志

  園芸学会令和4年度春季大会, Mar. 2022, Japanese

  Oral presentation


• ペチュニア花粉におけるニホンナシSFBB融合タンパク質発現系の開発

  上野環太, 久保健一, 藤本龍, 高山誠司, 安田(高崎)剛志

  園芸学会令和元年度秋季大会, Sep. 2019, Japanese

  Oral presentation


• ニホンナシSホモ系統の花粉RNAからのPpSFBB配列の再構築とクローニング

  野木彩未, 高畑宏基, 西村遼太郎, 藤本龍, 安田(高崎)剛志

  園芸学会令和元年度秋季大会, Sep. 2019, Japanese

  Oral presentation


• Vernalization alters histone H3 lysine 27 trimethylation at FLC locus in Brassica rapa

  Ayasha Akter, Satoshi Takahashi, Namiko Nishida, Naomi Miyaji, Takeshi Yasuda, Yutaka Suzuki, Motoaki Seki, FUJIMOTO RYO

  2019 KSBB & SABRAO International Conference on Plant Breeding for Sustainable Development, Jul. 2019, English, Kimdaejung Convention Center, Gwangju, International conference

  Poster presentation


• Identification of non-additively expressed genes at early developmental stages in an F1 hybrid cultivar of Chinese cabbage

  Hasan Mehraj, Kodai Matsuo, Motoki Shimizu, Takeshi Yasuda, FUJIMOTO RYO

  2019 KSBB & SABRAO International Conference on Plant Breeding for Sustainable Development, Jul. 2019, English, Kimdaejung Convention Center, Gwangju, International conference

  Poster presentation


• Transcriptome analysis indicate mechanisms underlying the DDM1-mediated heterosis in Arabidopsis thaliana

  Kodai Matsuo, Naomi Miyaji, Takeshi Yasuda, FUJIMOTO RYO

  International Symposium; Principles of pluripotent stem cells underlying plant vitality, May 2019, English, Tohoku University, International conference

  Poster presentation


• Characterization of the transcriptome in heterotic F1 hybrids in Arabidopsis thaliana

  Terumi Murakami, Kodai Matsuo, Naomi Miyaji, Takeshi Yasuda, FUJIMOTO RYO

  International Symposium; Principles of pluripotent stem cells underlying plant vitality 英文, May 2019, English, Tohoku University, International conference

  Poster presentation


• 萎黄病抵抗性・感受性ハクサイ系統における萎黄病菌感染時の免疫応答

  宮路 直実, 清水 元樹, 加治 誠, 安田 剛志, 岡崎 桂一, FUJIMOTO RYO

  平成31年度日本植物病理学会大会, Mar. 2019, Japanese, つくば国際会議場, Domestic conference

  Oral presentation


• ハクサイにおける全ゲノムメチル化解析

  FUJIMOTO RYO, 高橋 聡史, 鈴木 穣, 関 原明, 安田 剛志

  園芸学会平成３１年度春季大会, Mar. 2019, Japanese, 明治大学, Domestic conference

  Oral presentation


• Distribution of histone H3 lysine 27 trimethylation in different tissues of Brassica rapa

  アクタ アヤシャ, 宮路 直実, 安田 剛志, FUJIMOTO RYO

  園芸学会平成３１年度春季大会, Mar. 2019, English, 明治大学, Domestic conference

  Oral presentation


• ニホンナシSホモ系統における花柱内の和合・不和合花粉管伸長

  高橋侑也, FUJIMOTO RYO, TAKASAKI-YASUDA TAKESHI

  園芸学会平成30年度秋季大会, Sep. 2018, Japanese, 鹿児島大学, Domestic conference

  Oral presentation


• ニホンナシS3-RNase周辺領域のBACコンティグの拡張によるPpSFBB3の探索

  張競月, 武矢悠太郎, FUJIMOTO RYO, TAKASAKI-YASUDA TAKESHI

  平成30年度園芸学会近畿支部会大阪大会, Sep. 2018, Japanese, 大阪府立大学, Domestic conference

  Poster presentation


• Brassica rapa L.における萎黄病・根こぶ病抵抗性DNAマーカー選抜系の応用

  宮路 直実, Hasan Mehraj, 清水 元樹, 加治 誠, 安田, 高崎, 剛志, FUJIMOTO RYO

  平成30年度園芸学会近畿支部大阪大会, Sep. 2018, Japanese, 大阪府立大学 中百舌鳥キャンパス, Domestic conference

  Oral presentation


• Transcriptome Difference by Fusarium inoculation between Fusarium yellows resistant and susceptible lines in Brassica rapa L.

  Naomi Miyaji, Motoki Shimizu, Takeshi Takasaki-Yasuda, Keiichi Okazaki, FUJIMOTO RYO

  INTERNATIONAL PLANT MOLECULAR BIOLOGY 2018, Aug. 2018, English, International conference

  Poster presentation


• Characterization of epigenetic states in Brassica rapa L.

  FUJIMOTO RYO, Ayasha Akter, Satoshi Takahashi, Takeshi Takasaki-Yasuda, Yutaka Suzuki, Elizabeth S. Dennis, Motoaki Seki

  Brassica 2018, Jul. 2018, English, Saint-Malo, France, International conference

  Oral presentation


• Identification of long non-coding RNAs before and after prolonged cold treatment in Brassica rapa L.

  Namiko Nishida, Daniel J. Shea, Satoko Takada, Etsuko Itabashi, Takeshi Takasaki-Yasuda, Keiichi Okazaki, Elizabeth S. Dennis, FUJIMOTO RYO

  The 25th International Congress on Sexual Plant Reproduction, Jun. 2018, English, Nagaragawa Convention Center, International conference

  Poster presentation


• DDM1 has an important function on heterosis in Arabidopsis thaliana

  Naomi Miyaji, Etsuko Itabashi, Takeshi Takasaki-Yasuda, W. James Peacock, Elizabeth S. Dennis, FUJIMOTO RYO

  The 25th International Congress on Sexual Plant Reproduction, Jun. 2018, English, Nagaragawa Convention Center, International conference

  Poster presentation


• Characterization of histone H3 lysine 27 tri-methylation in Brassica rapa L.

  FUJIMOTO RYO, Ayasha Akter, Satoshi Takahashi, Namiko Nishida, Takashi Takasaki-Yasuda, Yutaka Suzuki, Motoaki Seki, Elizabeth S. Dennis

  The 25th International Congress on Sexual Plant Reproduction, Jun. 2018, English, Nagaragawa Convention Center, International conference

  Poster presentation


• 結球野菜の開花調節機構に関する研究の現状と今後の展望について

  FUJIMOTO RYO, 高田 紗都子, 西田 菜美子, 板橋 悦子, 安田 剛志, 柿崎 智博, 宇野 雄一

  園芸学会平成30年度春期大会, Mar. 2018, Japanese, 近畿大学農学部, Domestic conference

  Public symposium


• 萎黄病菌キャベツ分離菌抵抗性遺伝子DNAマーカーで萎黄病菌コマツナ分離菌抵抗性を予測できるか?

  宮路 直実, 清水 元樹, 加治 誠, 安田 (高, 崎, 剛志, 岡崎 桂一, FUJIMOTO RYO

  園芸学会平成30年度春期大会, Mar. 2018, Japanese, Domestic conference

  Poster presentation


• ハクサイ市販品種'W77'の両親系統間リシークエンスによる片親特異的遺伝子変異の同定

  上園 倖輔, Shea Daniel, 清水 元樹, 板橋 悦子, 宮路 直美, 岡崎 桂一, 安田, 高崎, 剛志, FUJIMOTO RYO

  園芸学会平成30年度春期大会, Mar. 2018, Japanese, Domestic conference

  Oral presentation


• Brassica rapaにおける春化経路関連遺伝子の機能解析

  西田 菜美子, 高田 紗都子, 板橋 悦子, 宮路 直実, 安田 剛志, FUJIMOTO RYO

  園芸学会平成30年度春期大会, Mar. 2018, Japanese, 近畿大学農学部, Domestic conference

  Oral presentation


• 萎黄病抵抗性•感受性ハクサイ系統における萎黄病菌感染時転写応答の解明

  宮路 直実, 清水 元樹, 高田 紗都子, 加治 誠, 安田, 高崎, 剛志, 岡崎 桂一, FUJIMOTO RYO

  園芸学会平成29年度秋期大会, Sep. 2017, Japanese, Domestic conference

  Oral presentation


• ハクサイ市販品種'W77'の両親系統のリシークエンスによるゲノム構造の系統間比較

  上園 倖輔, Shea Daniel, 清水 元樹, 板橋 悦子, 譚 永, 宮路 直美, 岡崎 桂一, 安田, 高崎, 剛志, FUJIMOTO RYO

  平成29年度園芸学会近畿支部滋賀大会, Sep. 2017, Japanese, Domestic conference

  Poster presentation


• ハクサイ市販品種'W77'における初期生育の雑種強勢関連領域の同定にむけて

  譚 永, 上園 倖輔, 板橋 悦子, 安田, 高崎, 剛志, FUJIMOTO RYO

  平成29年度園芸学会近畿支部滋賀大会, Sep. 2017, Japanese, ピアザ淡海, Domestic conference

  Oral presentation


• ニホンナシS2およびS4ハプロタイプ間のゲノム構造比較

  西村 遼太郎, 安藤 肇, FUJIMOTO RYO, 安田, 剛志

  園芸学会平成29年度秋期大会, Sep. 2017, Japanese, Domestic conference

  Oral presentation


• MeDIP-seqによるハクサイ系統間のDNAメチル化比較解析

  FUJIMOTO RYO, 高橋 聡史, 鈴木 穣, 関 原明, 安田 剛志

  園芸学会平成29年度秋期大会, Sep. 2017, Japanese, Domestic conference

  Oral presentation


• Brassica rapa L.における春化によるFLCのエピジェネティックな修飾変化の系統間比較

  高田 紗都子, 板橋 悦子, 宮路 直実, 安田 剛志, FUJIMOTO RYO

  園芸学会平成29年度秋期大会, Sep. 2017, Japanese, Domestic conference

  Oral presentation


• DDM1 is required for a full level of heterosis in Arabidopsis thaliana

  Naomi Miyaji, Sonoko Ishikura, Satoko Takada, Takeshi Takasaki-Yasuda, W. James Peacock, Elizabeth S. Dennis, FUJIMOTO RYO

  Plant Biology 2017, Jun. 2017, English, International conference

  Poster presentation


• ニホンナシ柱頭の表面および微細構造の変化と柱 頭浸出液の出現について

  高橋 侑也, 李 麗怡, 岡村 彩葉, FUJIMOTO RYO, 安田 (高, 崎, 剛志

  医学生物学電子顕微鏡技術学会第33回学術講演会, May 2017, Japanese, Domestic conference

  Oral presentation


• トランスクリプトーム解析を用いたハクサイの萎黄病菌感染時における免疫応答調査

  宮路 直実, 清水 元樹, 高田 紗都子, 加治 誠, 安田 剛志, 岡崎 桂一, FUJIMOTO RYO

  日本育種学会第131回講演会, Mar. 2017, Japanese, 名古屋大学, Domestic conference

  Oral presentation


• シロイヌナズナの早期開花エピ変異体の形質評価

  高田 紗都子, 石倉 園子, 板橋 悦子, 宮路 直実, 清水 元樹, 安田 剛志, FUJIMOTO RYO

  日本育種学会第131回講演会, Mar. 2017, Japanese, 名古屋, Domestic conference

  Oral presentation


• The epigenetic regulation of FLC expression by vernalization in Brassica rapa L

  Satoko Takada, Etsuko Itabashi, Naomi Miyaji, Takeshi Takasaki-Yasuda, Kenji Osabe, Elizabeth S. Dennis, FUJIMOTO RYO

  Brassica 2016, Oct. 2016, English, International conference

  Poster presentation


• Identification of the Fusarium yellows resistance genes; its application for marker-assisted selection in Brassica rapa

  Naomi Miyaji, Motoki Shimizu, Satoko Takada, Takeshi Takasaki-Yasuda, Makoto Kaji, Kenji Osabe, Elizabeth S. Dennis, Keiichi Okazaki, FUJIMOTO RYO

  Brassica 2016, Oct. 2016, English, International conference

  Oral presentation


• Genome-wide analysis of DNA methylation in Chinese cabbage

  Naoki Fukushima, Satoshi Takahashi, Etsuko Itabashi, Motoki Shimizu, Kenji Osabe, Takeshi Yasuda, Yutaka Suzuki, Motoaki Seki, Elizabeth S. Dennis, FUJIMOTO RYO

  Brassica 2016, Oct. 2016, English, International conference

  Poster presentation


• 萎黄病菌を接種したときの抵抗性と感受性ハクサイ系統のトランスクリプトームプロファイリング

  宮路 直実, 清水 元樹, 高田 紗都子, 加治 誠, 安田 剛志, 岡崎 桂一, FUJIMOTO RYO

  日本育種学会第130回講演会, Sep. 2016, Japanese, 鳥取大学, Domestic conference

  Poster presentation


• ハクサイにおける萎黄病菌感染時のトランスクリプトーム解析による病害反応遺伝子の調査

  宮路 直実, 清水 元樹, 高田 紗都子, 加治 誠, 安田 剛志, 岡崎 桂一, FUJIMOTO RYO

  園芸学会平成28年度秋季大会, Sep. 2016, Japanese, 名城大学, Domestic conference

  Oral presentation


• ハクサイにおけるメチローム解析

  福島 尚樹, 高橋 聡史, 板橋 悦子, 清水 元樹, 安田 剛志, 鈴木 穣, 関 原明, FUJIMOTO RYO

  日本育種学会第130回講演会, Sep. 2016, Japanese, Domestic conference

  Oral presentation


• ニホンナシS3-RNase 周辺BACコンティグの塩基配列解析

  武矢 悠太郎, 野村 直希, FUJIMOTO RYO, 安田, 剛志

  園芸学会平成28年度秋季大会, Sep. 2016, Japanese, 名城大学, Domestic conference

  Oral presentation


• ニホンナシS2遺伝子座のゲノム構造および発現遺伝子解析

  西村 遼太郎, FUJIMOTO RYO, 安田, 剛志

  園芸学会平成28年度秋季大会, Sep. 2016, Japanese, 名城大学, Domestic conference

  Oral presentation


• シロイヌナズナの雑種強勢に関与する遺伝子領域の探索

  宮路 直実, 石倉 園子, 高田 紗都子, 板橋 悦子, 安田 剛志, FUJIMOTO RYO

  日本育種学会第130回講演会, Sep. 2016, Japanese, Domestic conference

  Oral presentation


• クロマチンリモデリング因子DDM1によるエピジェネティックな制御がシロイヌナズナのヘテローシスに及ぼす影響

  石倉 園子, 宮路 直実, 板橋 悦子, 高田 紗都子, 高橋 聡史, 清水 元樹, 安田 剛志, 関 原明, FUJIMOTO RYO

  日本育種学会第130回講演会, Sep. 2016, Japanese, 鳥取大学, Domestic conference

  Oral presentation


• Brassica rapa L.の開花期の決定におけるFRIGIDAとFLOWERING LOCUS Cの役割

  高田 紗都子, 板橋 悦子, 宮路 直実, 安田 剛志, FUJIMOTO RYO

  日本育種学会第130回講演会, Sep. 2016, Japanese, Domestic conference

  Oral presentation


• Brassica rapa L.における春化によるFLOWERING LOCUS C のエピジェネティックな転写制御について

  高田 紗都子, 板橋 悦子, 宮路 直実, 清水 元樹, 安田 剛志, FUJIMOTO RYO

  園芸学会平成28年度秋季大会, Sep. 2016, Japanese, 名城大学, Domestic conference

  Poster presentation


• Brassica rapa L.における開花関連遺伝子FRIGIDA の発現解析および全長配列の決定

  高田 紗都子, 板橋 悦子, 宮路 直実, 安田 剛志, FUJIMOTO RYO

  園芸学会平成28年度秋季大会, Sep. 2016, Japanese, 名城大学, Domestic conference

  Oral presentation


• ニホンナシS3-RNase 周辺領域451kb のゲノム構造解析

  武矢 悠太郎, 野村 直希, FUJIMOTO RYO, 安田 剛志

  平成28 年度園芸学会近畿支部兵庫大会, Aug. 2016, Japanese, Domestic conference

  Oral presentation


• ニホンナシS2-RNase 周辺領域663kb のゲノム構造解析

  西村 遼太郎, FUJIMOTO RYO, 安田 剛志

  平成28 年度園芸学会近畿支部兵庫大会, Aug. 2016, Japanese, 神戸大学 瀧川記念学術交流会館, Domestic conference

  Oral presentation


• Brassica rapa L.における春化経路関連遺伝子FLOWERING LOCUS C の発現解析および エピジェネティックな転写制御について

  高田 紗都子, 板橋 悦子, 宮路 直美, 安田 剛志, FUJIMOTO RYO

  平成28 年度園芸学会近畿支部兵庫大会, Aug. 2016, Japanese, Domestic conference

  Oral presentation


• シロイヌナズナの雑種強勢の分子機構の解明に向けたRILの整備

  川辺 隆大, 宮路 直美, 高田 紗都子, 板橋 悦子, 安田 剛志, FUJIMOTO RYO

  日本育種学会第129回講演会, Mar. 2016, Japanese, 横浜市立大学, Domestic conference

  Oral presentation


• ハクサイにおけるヒストンメチル化抗体を用いたクロマチン免疫沈降法のポジティブ ネガティブコントロールプライマーの作製

  FUJIMOTO RYO, 川辺 隆大, 板橋 悦子, 安田 剛志

  平成27年度園芸学会秋季大会, Sep. 2015, Japanese, 園芸学会, 徳島大学, 概要, Domestic conference

  Oral presentation


• ハクサイにおけるヒストンメチル化抗体を用いたクロマチン免疫沈降法のポジティブ/ネガティブコントロールプライマーの作製

  FUJIMOTO RYO, 川辺 隆大, 板橋 悦子, 安田 剛志, 岡崎 桂一

  園芸学会平成27年度秋季大会, Sep. 2015, Japanese, Domestic conference

  Oral presentation


• ハクサイにおいて、両親系統間の遺伝距離から雑種強勢を予測できるか?

  川村 和隆, 佐伯 なつみ, 今野 周平, 永野 敦, 本庄 三恵, 八杉 公基, 工藤 洋, 安田 剛志, 加治 誠, 岡崎 桂一, FUJIMOTO RYO

  日本育種学会第128回講演会, Sep. 2015, Japanese, Domestic conference

  Oral presentation


• ハクサイにおいて、両親系統間の遺伝距離から雑種強勢を予測できるか?

  川村 和隆, 佐伯 なつみ, 今野 周平, 永野 敦, 本庄 三恵, 八杉 公基, 工藤 洋, 安田 剛志, 加治 誠, 岡崎 桂一, FUJIMOTO RYO

  育種学会第128回講演会, Sep. 2015, Japanese, 日本育種学会, 新潟大学, 概要, Domestic conference

  Oral presentation


• ニホンナシSホモ系統の花粉cDNAからのPpSFBB2ホモログのクローニングII

  西村 遼太郎, FUJIMOTO RYO, 安田 剛志

  平成27年度秋季大会, Sep. 2015, Japanese, 園芸学会, 徳島大学, 概要, Domestic conference

  Oral presentation


• ニホンナシ和合・不和合花粉管へのS-RNaseの取り込み

  李 麗怡, 時安 美奈, FUJIMOTO RYO, 安田 剛志

  平成26年度秋季大会, Sep. 2014, Japanese, 園芸学会, 佐賀大学, Domestic conference

  Oral presentation


• ニホンナシSホモ系統の花粉cDNAからのPpSFBB2ホモログのクローニング

  西村 遼太郎, 今村 剛士, 加藤 大貴, FUJIMOTO RYO, 安田 剛志

  平成26年度秋季大会, Sep. 2014, Japanese, 園芸学会, 佐賀大学, Domestic conference

  Oral presentation


• ニホンナシS4-RNase周辺BACコンティグの拡張によるPpSFBB遺伝子群の探索

  安藤 肇, 山下 翔人, FUJIMOTO RYO, 安田 剛志

  平成26年度秋季大会, Sep. 2014, Japanese, 園芸学会, 佐賀大学, Domestic conference

  Oral presentation


• ニホンナシnon-S-RNaseの花柱組織における蓄積と花粉管への取り込み

  時安 美奈, 李 麗怡, 岡村 彩葉, FUJIMOTO RYO, 安田 剛志

  平成26年度秋季大会, Sep. 2014, Japanese, 園芸学会, 佐賀大学, Domestic conference

  Oral presentation


• ニホンナシにおける自家・他家受粉後の花粉管微細構造の変化

  李麗怡, 時安美奈, 朴杓允, TAKASAKI-YASUDA TAKESHI

  園芸学会平成25年度秋季大会, Sep. 2013, Japanese, 園芸学, 岩手大学, Domestic conference

  Oral presentation


• S2-RNase周辺BACコンティグの拡張によるPpSFBB遺伝子群の探索II

  藤田裕樹, ズムラット クルバン, 齋藤寿広, TAKASAKI-YASUDA TAKESHI

  園芸学会平成25年度秋季大会, Sep. 2013, Japanese, 岩手大学, Domestic conference

  Oral presentation


• ニホンナシS4-RNase 周辺BAC コンティグの拡張による新規PpSFBB4 遺伝子の探索

  岡田和馬, 市川雄彦, TAKASAKI-YASUDA TAKESHI

  園芸学会平成25年度春季大会, Mar. 2013, Japanese, 園芸学会, 東京農工大学, Domestic conference

  Oral presentation


• ニホンナシ花柱組織の発育に伴うS-RNaseの蓄積

  岡村彩葉, 李麗怡, 石水毅, TAKASAKI-YASUDA TAKESHI

  園芸学会平成24年度秋季大会, Sep. 2012, Japanese, 福井県立大学, Domestic conference

  Oral presentation


• S2-RNase周辺BACコンティグの拡張によるPpSFBB遺伝子群の探索II

  藤田裕樹, TAKASAKI-YASUDA TAKESHI

  園芸学会平成24年度秋季大会, Sep. 2012, Japanese, Domestic conference

  Oral presentation


• ニホンナシ花柱組織の発育に伴う微細構造の変化

  岡村彩葉, 柏木由夏, 李麗怡, 朴杓允, TAKASAKI-YASUDA TAKESHI

  園芸学会平成23年度秋季大会, Sep. 2011, Japanese, 岡山大学, Domestic conference

  Oral presentation


• ニホンナシの柱頭浸出液中にもS-RNaseが含まれている

  木村穣, 太田文清, 石水毅, TAKASAKI-YASUDA TAKESHI

  園芸学会平成23年度秋季大会, Sep. 2011, Japanese, 岡山大学, Domestic conference

  Oral presentation


• ニホンナシにおける不和合花粉の発芽伸長は和合花粉に比べ遅延する

  山下翔人, TAKASAKI-YASUDA TAKESHI

  園芸学会平成23年度秋季大会, Sep. 2011, Japanese, 岡山大学, Domestic conference

  Oral presentation


• セイヨウナシ数品種からの新規Sx-RNaseのクローニング

  野村直希, 田中(守谷)友紀, TAKASAKI-YASUDA TAKESHI

  園芸学会平成23年度秋季大会, Sep. 2011, Japanese, 岡山大学, Domestic conference

  Oral presentation


• ニホンナシS2-及びS4-RNase周辺領域に存在するF-boxタンパク質遺伝子群の比較解析

  岡田和馬, 戸中希, 田口富雄, 市川雄彦, 澤村豊, 中西テツ, TAKASAKI-YASUDA TAKESHI

  園芸学会平成22年度秋季大会, Sep. 2010, Japanese, 大分大学, Domestic conference

  Oral presentation


• ニホンナシS3-RNase周辺域を含むBACクローンの配列解析

  野村直希, TAKASAKI-YASUDA TAKESHI

  園芸学会近畿支部会兵庫大会, Aug. 2010, Japanese, 神戸大学, Domestic conference

  Poster presentation


• ニホンナシ花柱内における不和合花粉管先端部の微細構造の変化

  河野友美, 山北由貴, 太田文清, 朴杓允, 中西テツ, TAKASAKI-YASUDA TAKESHI

  園芸学会平成21年度秋季大会, Sep. 2009, Japanese, 秋田大学, Domestic conference

  Oral presentation


• ニホンナシS4-RNase周辺BACコンティグの塩基配列解析

  岡田和馬, 戸中希, 市川雄彦, 澤村豊, 中西テツ, TAKASAKI-YASUDA TAKESHI

  園芸学会平成21年度秋季大会, Sep. 2009, Japanese, 秋田大学, Domestic conference

  Oral presentation


• Ultrastructural observation of the compatible and incompatible pollen tube in the style in S-homozygotes of the Japanese pear.

  Kono, Y, Yamakita ,Y, Ota, F, Park, P, Nakanishi,T, TAKASAKI-YASUDA TAKESHI

  6th International Symposium on Electron Microscopy in Medicine and Biology, Sep. 2009, English, Kobe University, International conference

  Poster presentation


• ニホンナシ在来品種 ‘千両’, ‘黒木’と ‘宝玉’のS遺伝子型同定

  岡田和馬, Calros Castillo, 澤村豊, 中西テツ, TAKASAKI-YASUDA TAKESHI

  園芸学会平成20年度秋季大会, Sep. 2008, Japanese, 三重大学, Domestic conference

  Oral presentation


• ニホンナシ花柱における自家・他家花粉管の微細構造の解析

  河野友美, 山北由貴, 太田文清, 朴 杓允, 中西テツ, TAKASAKI-YASUDA TAKESHI

  園芸学会平成20年度秋季大会, Sep. 2008, Japanese, 三重大学, Domestic conference

  Oral presentation


• ニホンナシS2-RNase周辺領域のBACコンティグの塩基配列解析

  田口富雄, 戸中希, 岡田和馬, 中西テツ, TAKASAKI-YASUDA TAKESHI

  園芸学会平成20年度秋季大会, Sep. 2008, Japanese, 三重大学, Domestic conference

  Oral presentation


• ニホンナシS4smハプロタイプにおける236kb欠失領域の塩基配列解析

  岡田和馬, 戸中希, 澤村豊, 中西テツ, TAKASAKI-YASUDA TAKESHI

  園芸学会平成19年度秋季大会, Sep. 2007, Japanese, 香川大学, Domestic conference

  Oral presentation


• ニホンナシS2-およびS3-RNase周辺領域のBACコンティグの構築

  戸中希, 岡田和馬, 澤村豊, 中西テツ, TAKASAKI-YASUDA TAKESHI

  園芸学会平成19年度秋季大会, Sep. 2007, Japanese, 香川大学, Domestic conference

  Oral presentation


• セイヨウナシ三倍体品種のS遺伝子型推定

  守谷友紀, 山本健太郎, 岡田和馬, 岩波宏, 古籐田信博, 中西テツ, TAKASAKI-YASUDA TAKESHI

  園芸学会平成19年度秋季大会, Sep. 2007, Japanese, 香川大学, Domestic conference

  Oral presentation


• S-genotype assignment of European pear cultivars using S-RNase specific CAPS marker.

  TAKASAKI-YASUDA, T, Moriya, Y, Iwanami, H, Bessho, H, Nakanishi, T

  10th International pear symposium, May 2007, English, ポルトガル, International conference

  Poster presentation


• Selection of self-compatible trees by S4sm-haplotype specific marker in Japanese pear.

  Okada, K, Tonaka, N, Sawamura, Y, Matumoto, T, TAKASAKI-YASUDA, T

  10th International pear symposium, May 2007, Japanese, International conference

  Poster presentation


• ニホンナシ‘長十郎’のBACライブラリーの作製によるS2-RNase周辺領域BACコンティグの構築

  Nozomi Tonaka, Takeshi Takasaki

  園芸学会平成18年度秋季大会, Sep. 2006, Japanese, 長崎大学, Domestic conference

  Oral presentation


• ニホンナシS4smハプロタイプ特異的マーカーによる自家和合性個体の選抜

  Kazuma Okada, Takeshi Takasaki

  園芸学会平成18年度秋季大会, Sep. 2006, Japanese, 長崎大学, Domestic conference

  Oral presentation


• セイヨウナシにおける自家摘果性の品種間差異

  Kentaro Yamamoto, Takeshi Takasaki

  園芸学会平成18年度秋季大会/日本園芸学会, Sep. 2006, Japanese, 長崎大学, Domestic conference

  Oral presentation


• Sa, Sb, Sc, Sd, Se, Sg, Sh, Si, Sk, Sl, Sm, Sn, Sp, Sq, Sr, Ss, St対立遺伝子を持つセイヨウナシ品種のS遺伝子型を推定するPCR-RFLPシステム

  Yuki Moriya, Takeshi Takasaki

  園芸学会平成18年度秋季大会, Sep. 2006, Japanese, 長崎大学, Domestic conference

  Oral presentation


• 遺伝子導入によるアブラナ科自家不和合性の制御に関する研究

  Takeshi Takasaki

  日本育種学会第109回講演会受賞講演, Mar. 2006, Japanese, 東京農工大学, Domestic conference

  Invited oral presentation


• ニホンナシS4smハプロタイプにおけるS4-RNase周辺欠失領域の同定

  Okada Kazuma, Takeshi Takasaki

  日本育種学会第109回講演会, Mar. 2006, Japanese, 東京農工大学, Domestic conference

  Oral presentation


• セイヨウナシにおける17種類のS対立遺伝子の全長 cDNAの単離・解析と遺伝子型を推定するPCR-RFLPシステムの開発

  Takeshi Takasaki, Yuki Moriya

  日本育種学会, Mar. 2006, Japanese, 東京農工大学, Domestic conference

  Oral presentation


• ニホンナシS4smハプロタイプにおけるS4-Rnase周辺欠失領域の解析

  岡田 和馬, 柏木 由夏, 守谷 友紀, 澤村 豊, 乗岡 茂巳, 中西 テツ, 高崎 剛志

  園芸学会平成17年度秋季大会＋園芸学雑誌74別2pp600, Oct. 2005, Japanese, 園芸学会, 東北大学, Domestic conference

  Oral presentation


• S1～S12対立遺伝子を持つセイヨウナシ品種のS遺伝子型推定

  守谷 友紀, 山本 健太郎, 岡田 和馬, 岩波 宏, 中西 テツ, 高崎 剛志

  園芸学会平成17年度秋季大会＋園芸学雑誌74別2pp601, Oct. 2005, Japanese, 園芸学会, 東北大学, Domestic conference

  Oral presentation


• ニホンナシS4-RNase 周辺領域のBACコンティグの構築とS4sm ハプロタイプの欠失領域の解析Ⅰ

  岡田 和馬, 柏木 由夏, 守谷 友紀, 澤村 豊, 乗岡 茂巳, 中西 テツ, 高崎 剛志

  園芸学会平成17年度春季大会＋園芸学雑誌74別1pp424, Apr. 2005, Japanese, 園芸学会, 筑波大学, Domestic conference

  Oral presentation


• セイヨウナシ品種’グランド・チャンピオン’の部分的自家和合性

  守谷 友紀, 高井 良裕, 山本 健太郎, 岡田 和馬, 伊藤 大雄, 塩崎 雄之輔, 岩波 宏, 継枝 良一郎, 中西 テツ, 高崎 剛志

  園芸学会平成17年度春季大会＋園芸学雑誌74別1pp425, Apr. 2005, Japanese, 園芸学会, 筑波大学, Domestic conference

  Oral presentation


• 西洋なし生産に他家受粉は必要か

  高崎 剛志

  西洋なし研究会 資料pp.9-10, Mar. 2005, Japanese, 独立行政法人農業・生物系特定産業技術研究機構果樹研究所, 山形県上山市, Domestic conference

  Oral presentation


• 受粉後の低温連続時間がニホンナシの花粉発芽に及ぼす影響Ⅱ

  安田 和弘, 太田 文清, 高崎 剛志, 中西 テツ

  平成16年度園芸学会近畿支部兵庫大会, 2004, Japanese, 園芸学会近畿支部, 神戸大学, Domestic conference

  Oral presentation


• ニホンナシ‘二十世紀’に由来するS4ホモ個体のBACライブラリーの作製

  岡田 和馬, 柏木 由夏, 守谷 友紀, 乗岡 茂巳, 中西 テツ, 高崎 剛志

  園芸学会平成16年度秋季大会, 2004, Japanese, 園芸学会, 静岡大学, Domestic conference

  Oral presentation


• ニホンナシの自殖後代におけるS遺伝子型の分離

  岡田 和馬, 守谷 友紀, 真野 隆司, 澤村 豊, 村田 謙司, 中西 テツ, 高崎 剛志

  平成16年度園芸学会近畿支部兵庫大会, 2004, Japanese, 園芸学会近畿支部, 神戸大学, Domestic conference

  Oral presentation


• セイヨウナシにおけるPCR-RFLPシステムの開発Ⅰ.S1~S7-遺伝子を持つ品種の遺伝子型推定

  守谷 友紀, 岡田 和馬, 加藤 秀一, 峯村 万貴, 山近 龍浩, 伊藤 大雄, 塩崎 雄之輔, 岩波 宏, 中西 テツ, 高崎 剛志

  園芸学会平成16年度秋季大会, 2004, Japanese, 園芸学会, 静岡大学, Domestic conference

  Oral presentation


• セイヨウナシS1-S7-Rnase 全長cDNAの単離と解析

  守谷 友紀, 岡田 和馬, 加藤 秀一, 峯村 万貴, 山近 龍浩, 伊藤 大雄, 塩崎 雄之輔, 岩波 宏, 中西 テツ, 高崎 剛志

  園芸学会平成16年度春季大会, 2004, Japanese, 園芸学会, 宇都宮大学, Domestic conference

  Oral presentation


• 交配に基づくセイヨウナシの不和合・和合の判定

  守谷 友紀, 高井 良裕, 加藤 秀一, 山近 龍浩, 伊藤 大雄, 塩崎 雄之輔, 真野 隆司, 中西 テツ, 高崎 剛志

  園芸学会平成15 年度春季大会+園芸学雑誌, Apr. 2003, Japanese, 園芸学会, 日本大学湘南キャンパス, Domestic conference

  Oral presentation


• ニホンナシ品種‘雲井’と‘世界一’のS 遺伝子型はS1S3である

  岡田 和馬, CARLOS Castillo, 齋藤 寿広, 乗岡 茂巳, 高崎 剛志, 中西 テツ

  園芸学会平成15 年度春季大会+園芸学雑誌, Apr. 2003, Japanese, 園芸学会, 日本大学湘南キャンパス, Domestic conference

  Oral presentation


• ニホンナシにおけるS9-RNase cDNA のクローニングとS1~S9 ハプロタイプを識別するPCR-RFLP システムの確立

  岡田 和馬, CASTILLA Carlos, 守谷 友紀, 齋藤 寿広, 澤村 豊, 乗岡 尚子, 乗岡 茂巳, 高崎 剛志, 中西 テツ

  育種会平成15 年度秋季大会+育種学研究, 2003, Japanese, 日本育種学会, 神戸大学, Domestic conference

  Oral presentation


• S-RNase に基づくセイヨウナシ(Pyrus communis L.)品種のS 遺伝子型の推定II S1~S12-RNase を持つ品種

  守谷 友紀, 高井 良裕, 岡田 和馬, 加藤 秀一, 峯村 万貴, 山近 龍浩, 伊藤 大雄, 塩崎 雄之輔, 中西 テツ, 高崎 剛志

  育種会平成15 年度秋季大会+育種学研究, 2003, Japanese, 日本育種学会, 神戸大学, Domestic conference

  Oral presentation


• PCR-RFLP system for distinguishing S1 to S9-haplotypes in Japanese pear (Pyrus pyrifolia Nakai)

  TAKASAKI Takeshi, OKADA K, CASTILLO Carlos, MORIYA Y, SAITO, SAWAMURA, NORIOKA, NAKANISHI Tetsu

  International Symposium on Plant Self-Incompatibility, 2003, English, 未記入, 未記入, International conference

  Oral presentation


• Cloning and characterization of two protein kinases expressed in pollen and pollen tubes of Nicotiana tabacum.

  DISSANAYAKE K, CASTILLO Carlos, TAKASAKI Takeshi, NAKANISHI Tetsu, NORIOKA N, NORIOKA S

  International Symposium on Plant Self-Incompatibility, 2003, English, 未記入, 未記入, International conference

  Oral presentation

• 医学生物学電子顕微鏡学会


• 国際園芸学会


• 園芸学会


• 日本育種学会

• Comprehensive analysis SFBB for S-RNase in self-incompatibility of Japanese pear

  安田 剛志, 井上 加奈子, 藤本 龍

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2019 - 31 Mar. 2023

  ナシ属の自家不和合性による花粉管の伸長抑制は雌ずい側因子S-RNaseが花粉管内のRNAを分解することで起こる。一方, 和合花粉管内では花粉側因子であるF-boxタンパク質群（SFBB）によりS-RNaseが無毒化されると考えられている。本研究では, SハプロタイプにコードされるSFBB群を掌握することで, S-RNaseに対する特異性を推定する。その推定をペチュニアの花粉で発現させたSFBB融合タンパク質のS-RNaseに対する結合特性を検証することを目的とした。 令和元年度は, ニホンナシのS2, S3, S4 BACコンティグ内外に存在する未解読BACクローンを次世代シークエンサーPacBioRSIIで解析し, 取得した配列を既知のコンティグに統合した。コンティグ配列はS2-RNase上流578kb~下流481kb, S3-RNase上流824kb~下流471kb, S4-RNase上流935kb~下流538kbまで拡張され, それぞれ18個, 19個, 20個のSFBBが同定された。各コンティグの両末端にはSFBB様配列は見出せなかったことから, これらSハプロタイプがコードするSFBB群を掌握できた。RNA-seqデータから構築したS1,S5-SFBB群配列の非翻訳領域から設計したプライマーセットを用いたRT-PCRにより未だクローニングされていないSFBB群配列をクローニングし, 配列を確認した。一方で, Skを有するニホンナシ品種 ‘巾着’の花粉をRNA-seq解析して, Sk-SFBB群配列を構築した。 S4-SFBB4d1およびその対立遺伝子のS1-SFBB4d1をそれぞれ導入したペチュニア形質転換を作出し, RT-PCRにより花粉での発現を確認した。 電子線トモグラフィー解析に向けた技術的な課題（試料作製法および連続傾斜撮影法など）を洗い出した。


• ニホンナシ自家不和合性における花粉管の伸長および伸長停止機構の包括的解析

  安田 剛志

  科学研究費補助金／基盤研究(B), Apr. 2015 - Mar. 2019, Principal investigator

  Competitive research funding


• ニホンナシ自家不和合性における花粉側Ｓ特異性の新展開

  安田 剛志

  科学研究費補助金／基盤研究(B), 2011, Principal investigator

  Competitive research funding


• ニホンナシ自家不和合性を制御するＳ遺伝子座の分子遺伝学的・組織学的解剖

  高崎 剛志

  科学研究費補助金／基盤研究(B), 2007

  Competitive research funding


• ニホンナシ自家不和合性における花粉側Ｓ遺伝子の分子的及び遺伝的解析の展開

  森本 テツ

  科学研究費補助金／基盤研究(B), 2005

  Competitive research funding


• セイヨウナシにおける結実管理システムの安定・省力化

  高崎 剛志

  科学研究費補助金／基盤研究(C), 2005

  Competitive research funding