Research activity information

• Oct. 2024 神戸大学, 令和６年度学長表彰, 財務貢献者

  辻野義雄


• Oct. 2023 神戸大学, 令和５年度学⻑表彰, 財務貢献者

  辻野義雄


• Oct. 2022 神戸大学, 令和４年度学⻑表彰, 財務貢献者

  辻野義雄


• Apr. 2019 In-cosmetics 2019 Paris, Innovation Zone Best Ingredient Award 2019, Category: Functional, Finalist

  Yoshio Tsujino


• Feb. 2019 一般財団法人 油脂工業会館, 油脂技術優秀論文賞

  辻野義雄

• Synthesis of a new amino acid derivative with long‐lasting hair shape control effects and elucidation of its mechanisms

  Sotaro Sato, Hinako Tabuchi, Kohtaro Katayama, Kenji Matsumoto, Hiroyuki Miyamoto, Sorai Kanno, Hiroshi Sekiguchi, Yoshinobu Tanaka, Mari Inoue, Choji Murata, Hiroki Hotta, Yoshio Tsujino

  Abstract Objective Hair is a crucial aspect of an individual's physical appearance. Thus, there is always a need for technology to care for curls and undulations. In this study, we found that heat treatment with a mixed aqueous solution of cysteamine (2‐aminoethanethiol) and fumaric acid was highly effective in improving hair quality. The purpose of this study was to scientifically elucidate the effectiveness of this technology by identifying its active ingredients, evaluating their functionality and analysing changes in the internal structure of hair after treatment. Method We observed changes in hair shape through a hair straightening test, analysed the components using liquid chromatography/mass spectrometry (LC/MS) and investigated the effects on mechanical properties using bending and surface friction measurements. Furthermore, we analysed the hair conditions using small‐angle X‐ray scattering (SAXS) measurements and evaluated water retention using thermal analysis. Results In the hair straightening test, even extremely strong curls of African human hair improved. The LC/MS results revealed that the active ingredient was 2‐(2‐aminoethylthio) succinic acid (ATS). Furthermore, by treating hair with ATS, decreases in the coefficient of friction and bending stiffness and an increase in moisture content were observed. The SAXS measurements revealed that the treatment widened the distance between the intermediate filaments (IFs) inside the hair and improved the orientation of the IFs. Conclusion ATS, produced by the thiol–ene reaction between fumaric acid and cysteamine, acts as an active ingredient in hair shape control. ATS reacted within the matrix and increased the IF–IF distance. This suggests that ATS functions as a crosslinker for keratin proteins. ATS is believed to increase the moisture content of hair and improve hair texture.

  Corresponding, Wiley, Feb. 2025, International Journal of Cosmetic Science, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Fraglide-1 from traditional Chinese aromatic vinegar: A natural AhR antagonist for atopic dermatitis.

  Kosuke Kato, Miki Akamatsu, Saya Kakimaru, Mayuko Koreishi, Masahiro Takagi, Masahiro Miyashita, Yoshiyuki Murata, Yoshimasa Nakamura, Ayano Satoh, Yoshio Tsujino

  Traditional Chinese Zhenjiang aromatic vinegar (Kozu) contains Fraglide-1 (FG1), a bioactive lactone with demonstrated peroxisome proliferator-activated receptor gamma (PPARγ) agonist and antioxidant activities. This study explored FG1's novel ability to antagonize the aryl hydrocarbon receptor (AhR) signaling pathway, which regulates artemin expression and contributes to itching and inflammation in atopic dermatitis. Through molecular docking simulations and cell-based assays in human keratinocytes, we demonstrated FG1's potent antagonistic activity against AhR signaling. FG1 effectively suppressed FICZ-induced inflammatory responses, including artemin expression, with potency (half maximal inhibitory concentration, IC50 = 5.1 μM) comparable to the synthetic antagonist StemRegenin 1 (SR1) while demonstrating a superior safety profile (median lethal concentration, LC50 > 100 μM vs. 27.5 μM for SR1). These findings expand our understanding of bioactive compounds from traditional fermented foods and their regulatory effects on AhR signaling, providing a foundation for future studies on FG1's role in modulating skin inflammation.

  Feb. 2025, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 115301 - 115301, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Resveratrol, a food-derived polyphenol, promotes Melanosomal degradation in skin fibroblasts through coordinated activation of autophagy, lysosomal, and antioxidant pathways

  Saki Okamoto, Saya Kakimaru, Mayuko Koreishi, Mika Sakamoto, Yoshimasa Nakamura, Hideya Ando, Yoshio Tsujino, Ayano Satoh

  Resveratrol, a polyphenol found in grapes and peanuts, is known for diverse biological activities, yet its effects on dermal hyperpigmentation (so-called dark spots) remain unexplored. We investigated resveratrol's ability to enhance melanosomal degradation in human dermal fibroblasts. At concentrations of 25–50 μM, resveratrol increased autophagy as measured by microtubule-associated protein 1A/1B-light chain 3 (LC3)-II/LC3-I ratio and enhanced lysosomal activity as assessed by a lysosomal activity reporter system. RNA sequencing revealed upregulation of lysosomal and autophagy-related genes, including cathepsins. Furthermore, reporter assays showed resveratrol's activation of antioxidant response via nuclear factor erythroid 2-related factor 2 (NRF2)-mediated, leading to upregulation of transcription factor EB/transcription factor E3 (TFEB/TFE3), master regulators of lysosomal function. In fibroblasts pre-loaded with melanosomes, resveratrol reduced melanosome content compared to control by day 3. The findings reveal the activation of interconnected autophagy, lysosomal, and antioxidant pathways by resveratrol, suggesting potential applications in functional foods targeting dermal hyperpigmentation.

  Feb. 2025, Journal of Functional Foods, 125, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Efficient Synthesis of cis,cis-Muconic Acid by Catechol Oxidation of Ozone in the Presence of a Base

  Kohtaro Katayama, Hiroki Hotta, Yoshio Tsujino

  Jan. 2025, Molecules2025, 30(1) (1), English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Bilirubin oxidase expression and activity enhancement from Myrothecium verrucaria in Aspergillus species

  Haruka Kado Horiguchi, Hironori Semba, Hiroyuki Yamada, Hirokazu Tsuboi, Takayuki Bogaki, Akio Koda, Kunishige Kataoka, Masahiro Takagi, Yoshio Tsujino

  Elsevier BV, Sep. 2024, Journal of Bioscience and Bioengineering, 138(3) (3), 212 - 217

  [Refereed]

  Scientific journal


• Aromatic oil from lavender as an atopic dermatitis suppressant

  Haruna Sato, Kosuke Kato, Mayuko Koreishi, Yoshimasa Nakamura, Yoshio Tsujino, Ayano Satoh

  In atopic dermatitis (AD), nerves are abnormally stretched near the surface of the skin, making it sensitive to itching. Expression of neurotrophic factor Artemin (ARTN) involved in such nerve stretching is induced by the xenobiotic response (XRE) to air pollutants and UV radiation products. Therefore, AD can be monitored by the XRE response. Previously, we established a human keratinocyte cell line stably expressing a NanoLuc reporter gene downstream of XRE. We found that 6-formylindolo[3,2-b]carbazole (FICZ), a tryptophan metabolite and known inducer of the XRE, increased reporter and Artemin mRNA expression, indicating that FICZ-treated cells could be a model for AD. Lavender essential oil has been used in folk medicine to treat AD, but the scientific basis for its use is unclear. In the present study, we investigated the efficacy of lavender essential oil and its major components, linalyl acetate and linalool, to suppress AD and sensitize skin using the established AD model cell line, and keratinocyte and dendritic cell activation assays. Our results indicated that lavender essential oil from L. angustifolia and linalyl acetate exerted a strong AD inhibitory effect and almost no skin sensitization. Our model is useful in that it can circumvent the practice of using animal studies to evaluate AD medicines.

  Public Library of Science (PLoS), Jan. 2024, PLoS one, 19(1) (1), e0296408 - e0296408, English

  [Refereed]

  Scientific journal


• Fucosyltransferase 8 (FUT8) and core fucose expression in oxidative stress response.

  Yuki M Kyunai, Mika Sakamoto, Mayuko Koreishi, Yoshio Tsujino, Ayano Satoh

  GlycoMaple is a new tool to predict glycan structures based on the expression levels of 950 genes encoding glycan biosynthesis-related enzymes and proteins using RNA-seq data. The antioxidant response, protecting cells from oxidative stress, has been focused on because its activation may relieve pathological conditions, such as neurodegenerative diseases. Genes involved in the antioxidant response are defined within the GO:0006979 category, including 441 human genes. Fifteen genes overlap between the glycan biosynthesis-related genes defined by GlycoMaple and the antioxidant response genes defined by GO:0006979, one of which is FUT8. 5-Hydroxy-4-phenyl-butenolide (5H4PB) extracted from Chinese aromatic vinegar induces the expression of a series of antioxidant response genes that protect cells from oxidative stress via activation of the nuclear factor erythroid 2-related factor 2-antioxidant response element pathway. Here, we show that FUT8 is upregulated in both our RNA-seq data set of 5H4PB-treated cells and publicly available RNA-seq data set of cells treated with another antioxidant, sulforaphane. Applying our RNA-seq data set to GlycoMaple led to a prediction of an increase in the core fucose of N-glycan that was confirmed by flow cytometry using a fucose-binding lectin. These results suggest that FUT8 and core fucose expression may increase upon the antioxidant response.

  2023, PloS one, 18(2) (2), e0281516, English, International magazine

  [Refereed]

  Scientific journal


• The Number of Electrons for the Oxidation of Caffeic Acid Depends on the Internal Volume of Electrolytic Cell

  Kenji Matsumoto, Sorai Kanno, Yuito Kitagawa, Yoshio Tsujino, Toshiyuki Osakai, Hiroki Hotta

  Jun. 2022, Journal of Flow Injection Analysis, 39(1) (1), English

  [Refereed]

  Scientific journal


• Evaluation of skin sensitization based on interleukin‑2 promoter activation in Jurkat cells

  Taichi Nagahata, Yoshio Tsujino, Eiji Takayama, Haruka Hikasa, Ayano Satoh

  Skin sensitization is an allergic reaction caused by certain chemical substances, and is an important factor to be taken into consideration when evaluating the safety of numerous types of products. Although animal testing has long been used to evaluate skin sensitization, the recent trend to regulate such testing has led to the development and use of alternative methods. Skin sensitization reactions are summarized in the form of an adverse outcome pathway consisting of four key events (KE), including covalent binding to skin proteins (KE1), keratinocyte activation (KE2), and dendritic cell activation (KE3). Equivalent alternative methods have been developed for KE1 to KE3, but no valid alternative has yet been developed for the evaluation of KE4 and T-cell activation. Current alternative methods rely on data from KE1 to KE3 to predict the effect of chemicals on skin sensitization. The addition of KE4 data is expected to improve the accuracy and reproducibility of such predictions. The aim of this study was to establish an assay to evaluate KE4 T-cell activation to supplement data on skin sensitization related to KE4. To evaluate T-cell activation, the Jurkat T-cell line stably expressing luciferase downstream of the pro-inflammatory cytokine interleukin-2 promoter was used. After exposure to known skin sensitizing agents and control substances, luciferase activity measurements revealed that this assay was valid for evaluating skin sensitization. However, two skin sensitizers known to have immunosuppressive effects on T-cells reacted negatively in this assay. The results revealed that this assay simultaneously allows for monitoring of the skin sensitization and immuno-suppressiveness of chemical substances and supplements KE4 T-cell activation data, and may thus contribute to reducing the use of animal experiments.

  Spandidos Publications, Jan. 2022, Biomedical Reports, 16(1) (1), 3 - 3, English, International magazine

  [Refereed]

  Scientific journal


• Membrane permeation of giant unilamellar vesicles and corneal epithelial cells with lipophilic vitamin nanoemulsions

  Miyuki Miyake, Yasushi Kakizawa, Norio Tobori, Masatoshi Kurioka, Nobuhito Tabuchi, Ryo Kon, Naofumi Shimokawa, Yoshio Tsujino, Masahiro Takagi

  Elsevier BV, Sep. 2018, Colloids and Surfaces B: Biointerfaces, 169, 444 - 452

  [Refereed]

  Scientific journal


• Unassuming softness silicone conditions hair without affecting dyes, perms of pores

  Y. Tsujino

  2018, Cosmetics & Toiletries, 133(10) (10), 46 - 56

  [Refereed]


• Nrf2/AREシグナル伝達経路の活性化による5-ヒドロキシ-4-フェニル-ブテノライドの抗酸化効果

  田部井 陽介, 室冨 和俊, 梅野 彩, 堀江 祐範, 辻野 義雄, 桝谷 文武, 吉田 康一, 中島 芳浩

  生命科学系学会合同年次大会運営事務局, Dec. 2017, 生命科学系学会合同年次大会, 2017年度, [2P - 0485], Japanese

  [Refereed]


• Raft-dependent endocytic movement and intracellular cluster formation during T cell activation triggered by concanavalin A

  Satomi Yabuuchi, Satoshi Endo, KeangOk Baek, Kunihide Hoshino, Yoshio Tsujino, Mun'delanji C. Vestergaard, Masahiro Takagi

  Elsevier BV, Dec. 2017, Journal of Bioscience and Bioengineering, 124(6) (6), 685 - 693

  [Refereed]

  Scientific journal


• Antioxidant properties of 5-hydroxy-4-phenyl-butenolide via activation of Nrf2/ARE signaling pathway

  Yosuke Tabei, Kazutoshi Murotomi, Aya Umeno, Masanori Horie, Yoshio Tsujino, Bumbu Masutani, Yasukazu Yoshida, Yoshihiro Nakajima

  5-Hydroxy-4-phenyl-butenolide (5H4PB) is a bioactive compound with antifungal and anti-obesity properties. Although it has recently been shown that 5H4PB activates peroxisome proliferator-activated receptor-gamma (PPARγ), the effect of 5H4PB on intracellular signaling pathways has not been clarified. In this study, we found that 5H4PB activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathway, which plays an important role in cellular defense against oxidative stress, and the subsequent upregulation of ARE-dependent cytoprotective genes, including the heme oxygenase-1, catalase, and superoxide dismutase genes, without exhibiting cytotoxicity. In addition, 5H4PB significantly attenuated intracellular ROS generation, glutathione oxidation, and DNA damage induced by hydrogen peroxide (H2O2) exposure in mouse fibroblast cells. Furthermore, we demonstrated that pretreatment with 5H4PB confers a significant cytoprotective effect against H2O2-induced cell death in mouse cultured fibroblasts and primary hepatocytes. Thus, our study demonstrated that 5H4PB enhanced cellular resistance to oxidative damage via activation of the Nrf2/ARE signaling pathway.

  Elsevier BV, Sep. 2017, Food and Chemical Toxicology, 107(Pt A) (Pt A), 129 - 137, English, International magazine

  [Refereed]

  Scientific journal


• Chirality-dependent interaction of d- and l-menthol with biomembrane models

  P.Gusain, S. Ohki, K. Hoshino, Y. Tsujino, N. Shimokawa, M. Takagi

  Chirality plays a vital role in biological membranes and has a significant effect depending on the type and arrangement of the isomer. Menthol has two typical chiral forms, d- and l-, which exhibit different behaviours. l-Menthol is known for its physiological effect on sensitivity (i.e., a cooling effect), whereas d-menthol causes skin irritation. Menthol molecules may affect not only the thermoreceptors on biomembranes, but also the membrane itself. Membrane heterogeneity (lipid rafts, phase separation) depends on lipid packing and acyl chain ordering. Our interest is to elaborate the chirality dependence of d- and l-menthol on membrane heterogeneity. We revealed physical differences between the two optical isomers of menthol on membrane heterogeneity by studying model membranes using nuclear magnetic resonance and microscopic observation.

  MDPI, 2017, Membranes 2017, 7(4) (4), 69 - 82, English

  [Refereed]

  Scientific journal


• A New Agonist for Peroxisome Proliferation-activated Receptor γ (PPARγ), Fraglide-1 from Zhenjiang Fragrant Vinegar: Screening and Characterization Based on Cell Culture Experiments

  Yoshio Tsujino

  Zhenjiang fragrant vinegar (Kozu) is a black rice vinegar that has been used as a traditional Chinese medicine and has various health benefits, including anti-obesity effects. In the present study, using luciferase reporter assays of PPARγ promoter activity, a novel ingredient from 8-year-old Kozu, 5-hydroxy-4-phenyl-butenolide, was isolated. The newly found agonist was named as "Fraglide-1". Moreover, in subsequent experiments, it was confirmed that fraglide-1 was an PPARγ agonist and it could increase expression level of the uncoupling protein (UCP)-1. Fraglide-1 was chemically synthesized and it was verified that expression of the PPARγ was increased in dose dependent manner. Although Kozu has been consumed globally as a functional food for thousands of years, the mechanisms behind its health effects have not been characterized. The active ingredient of Kozu was successfully found and the results unraveled a longtime mystery about Kozu for its beneficial health effect.

  Japan Oil Chemists' Society, 2017, Journal of Oleo Science, 66(6) (6), 615 - 622, English

  Scientific journal


• Electrochemical characterization of a unique, “neutral” laccase from Flammulina velutipes

  Kaori (Otsuka) Saito, Shinji Kurose, Yoshio Tsujino, Toshiyuki Osakai, Kunishige Kataoka, Takeshi Sakurai, Eiichi Tamiya

  The flac1 gene consisted of 1488 bases encodes a novel laccase (Flac1) from Flammulina velutipes. The deduced amino acid sequence of Flac1 with 496 amino acids shows 58-64% homologies with other fungal laccases. The recombinant Flac1 (rFlac1) was heterologously expressed in Pichia pastoris, with sugars of approximately 4 kDa attached on the protein molecule, which has the calculated molecular mass of 53,532 Da. rFlac1 was shown to be a multi-copper oxidase from spectroscopies. The optimum pHs of rFlac1 for oxidations of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), p-phenylenediamine, and o-aminophenol, were 5.0, 5.0, and 6.0-6.5, respectively, showing higher pH values than those from many other fungal laccases. The slightly acidic or neutral optimum pH that is not strongly dependent on substrates is a unique property of rFlac1. Effective O_2 reduction was realized by the direct electron transfer of rFlac1 at a highly oriented pyrolytic graphite electrode modified with fine carbon particles (Ketjen Black) in O_2-saturated solution. The pHs showing the maximum ΔE°' [E=E°'(enzyme) - E°'(substrate)] coincided well with the optimum pHs shown by rFlac1 under steady-state conditions. The present electrochemical results of rFlac1 indicate that ΔE°' is one of the primary factors to determine the activity of multi-copper oxidases.

  Corresponding, Elsevier BV, Feb. 2013, Journal of Bioscience and Bioengineering, 115(2) (2), 159 - 167, English

  [Refereed]

  Scientific journal


• γ-Pyronyl-triterpenoid Saponin (Chromosaponin I ) as an Electoron Donor

  Yoshio Tsujino

  Lead, Sep. 2012, Kobe University

  [Refereed]

  Doctoral thesis


• Isolation of a novel alkaline-induced laccase from Flammulina velutipes and its application for hair coloring

  Kaori (Otsuka) Saito, Ryuzo Ikeda, Katsunori Endo, Yoshio Tsujino, Masahiro Takagi, Eiichi Tamiya

  Corresponding, Elsevier BV, May 2012, Journal of Bioscience and Bioengineering, 113(5) (5), 575 - 579

  [Refereed]

  Scientific journal


• Physicochemical Profiling of Surfactant-Induced Membrane Dynamics in a Cell-Sized Liposome

  Tsutomu Hamada, Hideyuki Hagihara, Masamune Morita, Mun’delanji C. Vestergaard, Yoshio Tsujino, Masahiro Takagi

  American Chemical Society (ACS), Feb. 2012, The Journal of Physical Chemistry Letters, 3(3) (3), 430 - 435

  [Refereed]

  Scientific journal


• Triclosan–lysozyme complex as novel antimicrobial macromolecule: A new potential of lysozyme as phenolic drug-targeting molecule

  Md. Imranul Hoq, Koji Mitsuno, Yoshio Tsujino, Takayoshi Aoki, Hisham R. Ibrahim

  Elsevier BV, Jun. 2008, International Journal of Biological Macromolecules, 42(5) (5), 468 - 477, English

  [Refereed]

  Scientific journal


• A mechanistic study of the oxidation of natural antioxidants at the oil/water interface using scanning electrochemical microscopy

  Takayasu Sugihara, Tomoko Kinoshita, Shigeo Aoyagi, Yoshio Tsujino, Toshiyuki Osakai

  Elsevier BV, Jan. 2008, Journal of Electroanalytical Chemistry, 612(2) (2), 241 - 246, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Electrochemical consideration on the optimum pH of bilirubin oxidase

  Kaori Otsuka, Takayasu Sugihara, Yoshio Tsujino, Toshiyuki Osakai, Eiichi Tamiya

  Elsevier BV, Nov. 2007, Analytical Biochemistry, 370(1) (1), 98 - 106

  [Refereed]

  Scientific journal


• Physiological and Morphological Changes of the Facial Skin with Aging and Self-Consciousness in Japanese Men (Second Report)-Changes in the Wrinkle Formation and Facial Skin Color with Advancing Age and Men's Consciousness of Facial Cares-

  Ayumi Yamaguchi, Kazuyoshi Onishi, Ken-ichi Kuriyama, Yoshio Tsujino, Nobuki Fujiwara

  Japan Society of Cosmetic Chemists of Japan, 2007, Journal of Society of Cosmetic Chemists of Japan, 41(2) (2), 103 - 111

  [Refereed]

  Scientific journal


• Higher radical scavenging activities of polyphenolic antioxidants can be ascribed to chemical reactions following their oxidation

  Hiroki Hotta, Satomi Nagano, Masashi Ueda, Yoshio Tsujino, Junko Koyama, Toshiyuki Osakai

  Elsevier BV, Aug. 2002, Biochimica et Biophysica Acta (BBA) - General Subjects, 1572(1) (1), 123 - 132

  Scientific journal


• Mechanistic Study of the Oxidation of Caffeic Acid by Digital Simulation of Cyclic Voltammograms

  Hiroki Hotta, Masashi Ueda, Satomi Nagano, Yoshio Tsujino, Junko Koyama, Toshiyuki Osakai

  Elsevier BV, Apr. 2002, Analytical Biochemistry, 303(1) (1), 66 - 72

  Scientific journal


• Unusually large numbers of electrons for the oxidation of polyphenolic antioxidants

  Hiroki Hotta, Harumi Sakamoto, Satomi Nagano, Toshiyuki Osakai, Yoshio Tsujino

  Elsevier BV, May 2001, Biochimica et Biophysica Acta (BBA) - General Subjects, 1526(2) (2), 159 - 167

  Scientific journal


• Selective hydration of alkylammonium ions in nitrobenzene

  Akiko Ogata, Yoshio Tsujino, Toshiyuki Osakai

  Royal Society of Chemistry (RSC), 2000, Physical Chemistry Chemical Physics, 2(2) (2), 247 - 251

  Scientific journal


• Inhibitory effects on Epstein-Barr virus activation of anthraquinones: correlation with redox potentials

  J Koyama, K. Tagahara, T. Osakai, Y. Tsujino, S.Tsurumi, H. Nishino, H. Tokuda

  Elsevier BV, May 1997, Cancer Letters, 115(2) (2), 179 - 183

  Scientific journal


• Significance of Uricase in Oxidase-Induced Oxidative Coloring Reaction ofp-Phenylenediamine

  Masahiro Aoki, Yoshio Tsujino, Kenji Kano, Tokuji Ikeda

  American Chemical Society (ACS), Jan. 1996, The Journal of Organic Chemistry, 61(16) (16), 5610 - 5616, English

  [Refereed]

  Scientific journal


• Redox Properties of a γ-Pyronyl-Triterpenoid Saponin (Chromosaponin I)

  Yoshio Tsujino, Seiji Tsurumi, Taisaku Amakawa, Toshiyuki Osakai

  Lead, American Chemical Society (ACS), Dec. 1995, Journal of Natural Products, 58(12) (12), 1829 - 1839, English

  Scientific journal


• Chromosaponin I stimulates the growth of lettuce roots

  Seiji Tsurumi, Yoshio Tsujino

  A γ‐pyronyl triterpenoid saponin termed chromosaponin 1 (CSI), a conjugate of soyasaponin I and γ‐pyrone, was found at 3 mM to stimulate the growth of lettuce root (Lactuca sativa L. ev. Grand Rapids) to about 190% of the control. Since CSI is an amphipathic reductant, the stimulating effect of this saponin was compared with other reductants and other surface‐active compounds. Trolox (2‐carboxy‐2.5.7.8‐tetraamethyl‐6‐chromanol). another amphipathic reductant, also stimulated root growth, while other hydrophilic reductants including ascorbate. NADPH, NADH and glutathione did not. Some surfactants promoted root growth but their stimulating effects were smaller than the optimum effect of CSI. These results suggest a possible Function of CSI as an amphipathic reductant in root growth regulation.

  Wiley, Apr. 1995, Physiologia Plantarum, 93(4) (4), 785 - 789, English

  [Refereed]

  Scientific journal


• A New Triterpenoid Saponin (Chromosaponin I) with a Reducing Power

  Yoshio Tsujino, Seiji Tsurumi, Toshiyuki Osakai, Atsuyoshi Saito

  Abstract γ-Pyronyl-triterpenoid saponin (chromosaponin I; CSI) isolated from pea is strongly adsorbed at a glassy-carbon electrode, and gives an irreversible oxidation wave at 0.4 V vs. Ag/AgCl. The γ-pyronyl moiety is responsible for the electroactivity. CSI can reduce 1,4-benzoquinone, some of its derivatives and also cytochrome c. From comparison with other reductants, the formal redox potential of CSI was estimated to be 0.3–0.4 V vs. NHE (pH 7.0).

  Lead, Oxford University Press (OUP), Apr. 1994, Chemistry Letters, 23(4) (4), 711 - 714, English

  [Refereed]

  Scientific journal


• Antioxidative Effects of Dihydro-γ-pyronyl-triterpenoid Saponin (Chromosaponin I)

  Yoshio Tsujino, Seiji Tsurumi, Yasukazu Yoshida, Etsuo Niki

  Lead, Informa UK Limited, Jan. 1994, Bioscience, Biotechnology, and Biochemistry, 58(9) (9), 1731 - 1732, English

  [Refereed]

  Scientific journal


• Hair coloring and waving using oxidases

  YOSHIO TSUJINO, YOSHIHARU YOKOO, KUNIAKI SAKATO

  Lead, Jul. 1991, Journal of the society of cosmetic chemists, 42, 273 - 282, No password

  [Refereed]

  Scientific journal

  添付ファイルのURL


• The Application of Oxidases to Hair Dyeing and Permanent Waving

  Yoshio Tsujino, Kouji Kitayama, Yoshiharu Yokoo, Kuniaki Sakato

  Lead, Mar. 1991, Journal of the society of cosmetic chemists, 24(3) (3), 220 - 223, No password

  Scientific journal

  添付ファイルのURL

• Tribology for Hair Care Using Bioconjugation Technology

  Yoshio Tsujino

  Lead, Apr. 2020, トライボロジスト, 65(4) (4), 215 - 223, Japanese

  Introduction scientific journal


• グリオキシル酸による酸化染料の脱色作用

  辻野義雄, 宮里朗夫, 高木昌宏

  2020, 毛髪科学, (125) (125), 43 - 46

  [Refereed]

  Introduction other


• 3P-229 Evaluation of irritation by surfactants based on membrane dynamics

  Shimokawa Shinji, Shimokawa Naofumi, Tsujino Yoshio, Takagi Masahiro

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 328 - 328, Japanese

  [Refereed]


• 3P-236 Effect of temperature on lipid raft and cytoskeleton of T-cell

  Okubo Yufu, Yabuuchi Satomi, Baek KeangOk, Hoshino Kunihide, Tujino Yoshio, Shimokawa Naohumi, Takagi Masahiro

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 329 - 329, Japanese

  [Refereed]


• 2P-274 Physical changes induced by optical isomers of menthol on membrane dynamics

  Pooja Gusain, Kunihide Hoshino, Yoshio Tsujino, Naofumi Shimokawa, Masahiro Takagi

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 243 - 243, English


• ヘアカラーの変遷と技術動向

  辻野義雄

  Lead, 2015, トライポロジスト, 60(8) (8), 518 - 526

  Introduction scientific journal


• Development of new hair dyeing techniques without hydrogen peroxide

  OKAMURA AKINORI, TSUJINO YOSHIO

  Apr. 2014, Fragrance Journal, 42(3) (3), 17 - 22, Japanese

  [Refereed]

  Introduction scientific journal


• Variation of Skin Condition by Ingestion of Sake-condensate

  山下裕司, 山崎舞, 滝澤毅, 辻野義雄, 広常正人, 田上八朗, 坂本一民

  日本酒は日本古来のアルコール飲料として愛飲されており、適量飲酒が健康維持に役立つことが知られている。ストレス解消や血行促進は実感できる効果であり、近年では肝保護作用や美容効果も示唆されている。また、最近の研究からは日本酒に含まれる成分のエチルα-D-グルコシドが皮膚に対し整肌作用を有することが明らかにされている。本研究では日本酒からアルコールを取り除いた濃縮物に着目し、この濃縮物を配合したゼリー状飲料を摂取した時の皮膚性状の変化について被験者33名に対し臨床試験を実施した。設定した評価期間において、日本酒脱アルコール濃縮物の摂取に伴う角層水分量および経皮水分蒸散量への効果は見られなかったが、アンケート調査から肌質改善を感じたコメントが得られた。試料の味・香りから試験品とプラセボを認識したことにより、日本酒の有効成分に関する事前説明を受けた被験者は心理的効果を誘導されたと推測される。Sake is one of the favorite and traditional alcohol beverages for Japanese, and it is well known that an appropriate amount of drinking plays a role in maintaining healthy conditions. Stress release and stimulation of blood circulation by ingesting Sake is well accepted, and its effect on hepatic protection and skin condition have been recently suggested. Recent studies showed that a specific component, ethyl α-D-glucoside, contained in Sake can improve the skin condition.Therefore, the objective of present study is to clarify the effect of ingestion of the jelly combined with the Sake-condensate excluding alcohol for the skin condition. As a result, any of the effects of the Sake-condensate on the skin condition were not found, that is, the changes in the moisture content in the stratum corneum and the transepidermal water loss were almost independent on the jellys with or without the Sake-condensate during the examination period, while some subjects commented perceiving improvement of the skin condition. The taste and fl avor of the jellys might have lead the subjects to recognize the sample and placebo, to lead the subjects to perceive sample with the Sake-condensate effective to skin condition.

  千葉科学大学, 2014, 千葉科学大学紀要, 7(7) (7), 97 - 104, Japanese

  [Refereed]


• Technology to Smoothen Hair Surface with Shampoo Formulation

  辻野義雄, 上門潤一郎, 西垣祥子

  Lead, 日本トライボロジー学会 ; 1989-, 2014, トライポロジスト, 59(8) (8), 477 - 483, Japanese

  Introduction scientific journal


• 新規ヘアカラーリング技術への挑戦

  辻野義雄

  Lead, Oct. 2012, 毛髪科学, 111, 16 - 21, Japanese

  Introduction scientific journal


• Age-Associated Changes in Facial Skin and Suitable Skincare Differences between Adult Men and Women

  Onishi K., Yamaguchi A., Kuriyama K., Tsujino Y., Okada K.

  31 Mar. 2009, Journal of Japanese Cosmetic Science Society

  [Refereed]

  Report scientific journal


• Promotion of laccase activities of Escherichia coli cuprous oxidase, CueO by deleting the segment covering the substrate binding site

  Shinji Kurose, Kunishige Kataoka, Kaori Otsuka, Yoshio Tsujino, Takeshi Sakurai

  Total and partial deletions of the region covering the substrate-binding site of the Escherichia coli cuprous oxidase, CueO evidenced that the unique specificity for cuprous ion originates in the steric hindrance to interfere the access of laccase substrates towards the type I Cu site.

  CHEMICAL SOC JAPAN, Feb. 2007, CHEMISTRY LETTERS, 36(2) (2), 232 - 233, English


• 天然抗酸化剤クロロゲン酸の反応電子数について

  堀田 弘樹, 永野 里実, 大堺 利行, 辻野 義雄

  30 Oct. 1998, ポーラログラフィー, 44(3) (3), 117 - 117, Japanese


• 天然抗酸化剤クロロゲン酸の電気化学的研究

  堀田 弘樹, 坂本 晴美, 大堺 利行, 辻野 義雄

  07 Nov. 1997, ポーラログラフィー, 43(3) (3), 116 - 116, Japanese


• 酵素を用いたヘアカラーの開発

  辻野義雄

  Lead, Oct. 1995, フレグランスジャーナル (FRAGRANCE JOURNAL), Japanese

  Introduction scientific journal

• Cosmetic Science 12月号

  辻野義雄

  Contributor, 11.毛髪の加齢変化を改善する、持続型ヘアケア技術, Dec. 2024


• Cosmetic Science 12月号

  新菜摘, 辻野義雄, 堀田弘樹

  Contributor, 04.うねり改善成分の評価と製剤化応用, Dec. 2024


• SHINBIYO 10月号

  辻野義雄, 田中良宣

  Contributor, 緊急企画！トステア(R)って何ですか？, 新美容出版, Sep. 2024


• SHINBIYO 9月号

  辻野義雄

  Contributor, ダメージの仕組みと最新ケア成分, 新美容出版株式会社, Aug. 2024


• Hair Care Products: Cutting Edge Technology and Market Forecast

  Yoshio Tsujino

  Supervisor, シーエムシー出版, May 2023


• 健康の科学知識 : 食事、睡眠、美容、体調・・・・・・、知って役立つ体の知識50

  辻野義雄

  Single work, pp.53-56, ニュートンプレス, Jan. 2021, Japanese, ISBN: 9784315523096


• 体の科学知識 : 体にまつわる身近な"?"と対処法

  辻野義雄

  Single work, pp.112-115, ニュートンプレス, Dec. 2018, Japanese, ISBN: 9784315521337


• お客様のお悩み別「美容成分」事典 経営とサイエンス

  辻野義雄, 久光一誠

  Joint work, pp.12－47, 新美容出版, 2017


• がん患者に対するアピアランスケアの手引き

  国立がん研究センター研究開発費がん患者の外見支援に関するガイドラインの構築に向けた研究班

  Single work, CQ 37, pp.135-138，CQ 42, pp.146-148，CQ 43, pp.149-150，CQ 44, pp.151-154, 金原出版, Aug. 2016, Japanese, ISBN: 9784307702003


• コスメティックサイエンス : 化粧品の世界を知る

  辻野義雄

  Single work, 第13章「頭髪（ヘアケア）化粧品」, 共立出版, Jun. 2014, Japanese, ISBN: 9784320061774


• ナノ融合による先進バイオデバイス

  高木昌宏, 濱田勉, 萩原秀幸, 辻野義雄

  Joint work, 第2章 ナノバイオテクノロジー，2. 巨大リポソームの界面活性剤誘起ダイナミクス, シーエムシー出版, Nov. 2011, Japanese, ISBN: 9784781304878

• ヘアケア処方の変遷と技術動向～処方開発におけるトラブル事例とその対処を交えながら～

  辻野義雄

  R＆D支援センター, Mar. 2025

  [Invited]

  Public discourse


• 毛髪用ブリーチ剤におけるアルカリ臭の低減の検討

  吉井舞耶, 佐藤聡太郎, 片山拓哉, 池内亮太, 堀田弘樹, 辻野義雄, 鷲家真吾

  日本化学会第105春季年会（2025）, Mar. 2025

  Poster presentation


• Activation of bilirubin oxidase expressed in Escherichia coli by ascorbic acid treatment under anaerobic conditions

  Nanako IE, Haruka KADO, Satoshi YAMASHITA, Yoshio TSUJINO, Kunishige KATAOKA

  日本農芸化学会2025年度大会, Mar. 2025

  Poster presentation


• レスベラトロールによるオートファジー・抗酸化経路の活性化を介した皮膚線維芽細胞のメラノソーム分解促進効果：食品機能性成分としての可能性

  辻野義雄, 佐藤あやの

  日本農芸化学会2025年度札幌大会, Mar. 2025

  Poster presentation


• 加齢に伴う毛髪の用紙の変化を改善する新規ヘア素材『トステア®』とは

  辻野義雄

  第65回関西油化学講習会（油技術講座）, Dec. 2024

  [Invited]

  Public discourse


• Melanosome Uptake and Degradation by Dermal Fibroblast Cell Line: Activation of Autophagy and Antioxidant Pathways by Resveratrol

  Ayano Satoh, Yoshio Tsujino

  日本動物実験代替法学会第37回大会, Nov. 2024

  Poster presentation


• Oxidation reaction mechanism of caffeic acid under UV irradiation

  Yuito Kitagawa, Sorai Sorai, Kenji Matsumoto, Yoshio Tsujino, Hiroki Hotta

  The International Meeting of the Polarographic Society of Japan, Nov. 2024, English

  Poster presentation


• Reaction mechanism of polyphenols containing reactivating metadihydroxybenzene moiety

  Sorai Kanno, Yuito Kitagawa, Mayo Shioji, Kenji Matsumoto, Tomohiko Kuwabara, Hitoshi Mizuguchi, Yoshio Tsujino, Toshiyuki Osakai, Hiroki Hotta

  The International Meeting of the Polarographic Society of Japan, Nov. 2024, English

  Poster presentation


• 新規アミノ酸様化合物のうねり毛髪の改善効果や縮毛矯正効果の解明

  望月佑次, 井上翔太, 新菜摘, 川口慶晃, 西山美穂, 佐藤聡太郎, 片山耕太郎, 松本健嗣, 堀田弘樹, 辻野義雄

  第29回日本臨床毛髪学会学術集会, Nov. 2024, Japanese

  Oral presentation


• Development and evaluation of innovative new amino-acid-like compounds that improve hair frizz and curl retention by bioconjugation with hair tissue

  Inoue Shota, Shin Natsumi, Kawaguchi Yoshiaki, Fujiwara Akiyori, Katayama Kotaro, Sato Sotaro, Hotta Hiroki, Tsujino Yoshio, Mochizuki Yuji

  The 34th IFSCC Congress Brazil, Oct. 2024, English

  Poster presentation


• Beyond Straightening novel amino-acid-like compounds as a Groundbreaking Ingredient in Modern Hair Relaxer

  Yoshiaki Kawaguchi, Sotaro Sato, Shota Inoue, Kyoko Takahashi, Yuji Mochizuki, Akiyori Fujiwara, Hiroki Hotta, Yoshio Tsujino

  The 34th IFSCC Congress Brazil, Oct. 2024, English

  Poster presentation


• A Novel method for damage mitigation during hair bleaching by using the amino acid derivative

  Sotaro Sato, Katayama Kohtaro, Hiroki Hotta, Masahiro Takagi, Yoshio Tsujino

  The 34th IFSCC Congress Brazil, Oct. 2024, English

  Poster presentation


• Novel hair coloring system using eumelanin precursor, DHI (5,6-Dihydroxyindole) based on Hair Science

  Yuji Mochizuki, Akiyori Fujiwara, Tohru Nagahama, Anju Yamada, Hiroki Hotta, Yoshio Tsujino

  The 34th IFSCC Congress Brazil, Oct. 2024, English

  Oral presentation


• Analysis of redox reaction ofcaffeic acid under UV irradiation by electrochemical measurements

  Y. Kitagawa, S. Kanno, K. Matsumoto, Y. Tsujino, T. Osakai, H. Hotta

  PRiME 2024 Hawaii, Oct. 2024, English

  Poster presentation


• Analysis of Redax Properties of Polyphenols with Resorcinol Moiety

  S. Kannno, Y. Kitagawa, K. Matsumoto, T. Kuwabara, H. Mizuguchi, Y.Tsujino, T. Osakai, H. Hotta

  PRiME 2024 Hawaii, Oct. 2024, English

  Poster presentation


• 天然由来成分による皮膚や毛髪へのアンチエイジング効果

  辻野義雄

  2024年日中化粧品国際交流イベント, Sep. 2024

  [Invited]

  Public discourse


• メタジヒドロキシベンゼン骨格の含むポリフェノールの再活性化

  菅野宙依, 塩路万葉, 北川維人, 松本健嗣, 桑原知彦, 水口仁志, 辻野義雄, 大堺利行, 堀田弘樹

  日本分析化学会第73年会, Sep. 2024

  Oral presentation


• 光異性化した天然抗酸化剤カフェイン酸の酸化還元機構の研究

  北川維人, 菅野宙依, 松本健嗣, 辻野義雄, 堀田弘樹

  日本分析化学会第73年会, Sep. 2024

  Oral presentation


• 髪質改善素材とアミノ酸との反応性の観察

  宮本紘幸, 片山耕太郎, 佐藤聡太郎, 松本健嗣, 田中良宜, 生田義倫, 望月祐次, 新菜摘, 堀田弘樹, 辻野義雄

  日本分析化学会第73年会, Sep. 2024

  Poster presentation


• Myrothecium verrrucaria由来ビリルビンオキシダーゼのAspergillus属糸状菌での高生産と耐熱化

  加戸悠, 仙波弘雅, 山田尚平, 坪井宏和, 坊垣隆之, 幸田明生, 石川一彦, 森裕太郎, 荻野千秋, 高木昌宏, 辻野義雄

  第78回日本生物工学会大会

  Oral presentation


• 界面活性剤が誘起するかゆみの評価とその抑制メカニズム

  萬代由莉恵, 安鋼, 高瀬修一, 高木昌宏, 中谷祐将, 辻野義雄, 吉村倫一

  第62回日本油化学会年会, Sep. 2024

  Poster presentation


• Effects of Novel Amino Acid Compounds on Hair Bleaching Damage

  佐藤聡太郎, 宮本紘幸, 堀田弘樹, 生田義倫, 辻野義雄

  第49回日本香粧品学会, Jun. 2024

  Oral presentation


• Evaluation of the hair quality improvement effect of 2-(2-aminoethylthio) succinic acid

  宮本紘幸, 田渕日奈子, 佐藤聡太郎, 片山耕太郎, 松本健嗣, 田中良宣, 生田義倫, 堀田弘樹, 辻野義雄

  第49回日本香粧品学会, Jun. 2024

  Poster presentation


• Myrotheciyn verrucaria 由来ビリルビンオキシターゼのAspergillus属糸状菌での高生産と特性解析

  加戸悠, 仙波弘雅, 山田浩之, 坪井宏和, 坊垣隆之, 幸田明生, 片岡邦重, 高木昌弘, 辻野義雄

  日本農芸化学会2024年度大会, Mar. 2024

  Oral presentation


• 毛髪トリートメント効果を有する有機酸とアミンの新規化合物合成

  片山耕太郎, 宮本紘幸, 望月佑次, 新菜摘, 山崎祥子, 堀田弘樹, 辻野義雄

  日本化学会第104春季年会(2024), Mar. 2024

  Oral presentation


• 針葉樹チップ発酵熟成堆肥より得た腐植物質の抗酸化活性評価

  蔦保侑樹, 小嶋康詞, 堀田弘樹, 辻野義雄

  日本化学会第104春季年会(2024), Mar. 2024

  Oral presentation


• 有機酸とアミンを用いた新規化合物の合成と機能性の研究

  片山耕太郎, 宮本紘幸, 堀田弘樹, 山崎祥子, 田中良宜, 辻野義雄

  日本化学会秋季事業第13回CSJフェスタ2023, Oct. 2023, Japanese

  Poster presentation


• 有機酸-アミン複合体を用いた縮毛矯正とその毛髪の物性評価法に関する研究

  田渕日奈子, 片山耕太郎, 佐藤聡太郎, 松本健嗣, 堀田弘樹, 辻野義雄, 田中良宣, 関口博史, 井上真理, 村田長嗣

  日本分析化学会第72年会, Sep. 2023

  Oral presentation


• 蛍光色素を用いた毛髪の表面状態の観察

  山田杏樹, 佐藤聡太郎, 堀田弘樹, 辻野義雄, 中川充, 懸橋理枝, 望月佑次

  日本分析化学会第72年会, Sep. 2023

  Oral presentation


• Visualization and Evaluation Method for UV Cosmetics Application Using Motion Sensors

  Yoshikawa Riho, Izumi Shintaro, Terada Tsutomu, Tsujino Yoshio, Kawaguchi Hitoshi

  The 33rd IFSCC (International Federation of Societies of Cosmetic Chemists) Congress 2023 Barcelona Spain, Sep. 2023, English

  Poster presentation


• Surfactant-induced skin delicate irritation and its evaluation by human stinging test and model membrane dynamics

  Yurie Mandai, Gang An, Shuichi Takase, Masahiro Takagi, Yusuke Nakatani, Naofumi Shimokawa, Yoshio Tsujino

  The 33rd IFSCC (International Federation of Societies of Cosmetic Chemists) Congress 2023 Barcelona Spain, Sep. 2023, English

  Poster presentation


• Mechanistic Study of the Oxidation Reaction of Caffeic Acid Under UV Irradiation

  Yuito Kitagawa, Sorai Kanno, Kenji Matsumoto, Yoshio Tsujino, Toshiyuki Osakai, Hiroki Hotta

  The 31st International Conference on Photochemistry (ICP 2023 Sapporo Japan), Jul. 2023, English

  Poster presentation


• 髪質改善素材の研究に関わる分析化学

  佐藤聡太郎, 片山耕太郎, 田渕日奈子, 山田杏樹, 池内亮太, 宮本紘幸, 松本健嗣, 堀田弘樹, 辻野義雄

  日本分析化学会近畿支部創設70周年記念式典, Jun. 2023

  Poster presentation


• Surfactant-induced membrane dynamics and skin irritation

  M.Takagi, Y.Nakatani, N. Shimokawa, Y.Mandai, G.An, S.Takase, Y.Tsujino

  The 32nd IFSCC(International Federation of Societies of Cosmetic Chemists) Congress 2022, London, Sep. 2022, English

  Poster presentation


• 産学連携のための自然科学研究における経営学～コスメティックサイエンスを例に～

  辻野義雄

  日本生物工学会中部支部若手研究者による中部支部バイオセミナーin富山, Aug. 2022

  Public discourse


• 細胞膜模倣系を用いた界面活性剤刺激性評価

  中谷祐将, 伊藤太一, 下川直史, 萬代由梨恵, 安鋼, 高瀬修一, 辻野義雄, 高木昌宏

  日本化学会第102回春季大会, web開催, Mar. 2022


• 有機酸ーアミン複合体を用いた新規縮毛矯正剤の開発

  田渕日奈子, 佐藤聡太郎, 堀田弘樹, 辻野義雄, 生田義倫, 田中良宣

  日本化学会第102回春季大会, web開催, Mar. 2022


• マイクロバブルの頭髪への利用

  佐藤聡太郎, 堀田弘樹, 辻野義雄

  第12回毛髪科学研究発表会, web開催, Mar. 2022


• システアミンー有機酸複合体を用いた髪質改善効果について

  生田義倫, 田中良宣, 辻野義雄

  第12回毛髪科学研究発表会, web開催, Mar. 2022


• ヘアケア処方の変遷と技術動向

  辻野義雄

  第58回SCCJセミナー, Feb. 2022


• バイオコンジュゲーション技術を用いた新しい縮毛矯正技術

  佐藤聡太郎, 堀田弘樹, 辻野義雄

  若手フロンティア研究会2021, Dec. 2021


• Studies about membrane dynamics towards evaluation of skin irritation

  Masahiro Takagi, Naofumi Shimokawa, Yoshio Tsujio

  The International Chemical Congress of Pacific Basin Socienties 2021 （Pacifichem 2021）, Dec. 2021, English


• カフェイン酸のクーロメトリー検出における電解セル依存性について

  菅野宙依, 松本健嗣, 辻野義雄, 大堺利行, 堀田弘樹

  第67回ポーラログラフィーおよび電気分析化学討論会、web開催, Nov. 2021


• 香醋由来の機能性成分“フレグライド１”によるスカルプケアへのアプローチ

  辻野義雄

  第12回毛髪技術者協会「毛髪科学研究発表会」, Nov. 2021

  [Invited]


• ヘアカラーリングにおけるポリマーの活用

  辻野義雄

  第30回ポリマー材料フォーラム（主催：高分子学会）, Nov. 2021

  [Invited]


• 糸状菌における撹拌翼形状とタンパク質生産分泌生産の相関解析

  小野太暉, 鈴木智大, 加戸悠, 坪井宏和, 坊垣隆之, 幸田明夫, 辻野義雄, 近藤昭彦, 荻野千秋

  第73回日本生物工学会, Oct. 2021


• イオンドライヤーが毛髪に与える効果の評価

  佐藤聡太朗, 堀田弘樹, 中川充, 懸橋理枝, 戸塚広乃, 日野祥子, 辻野義雄

  日本分析学会第70年会, Sep. 2021


• 糸状菌を用いた外来性酸化還元酵素の分泌生産性の解析

  小野太暉, 近藤昭彦, 荻野千秋, 櫻川拓, 辻野義雄, 仙波弘雅, 坪井宏和, 坊垣隆之

  第23回化学工学会学生発表会, Mar. 2021


• アトピー性皮膚炎惹起性の評価のための動物実験代替法の確立

  加藤浩介, 辻野義雄, 佐藤あやの

  日本動物実験代替法学会第33回大会, Nov. 2020


• アトピー性皮膚炎惹起性評価のための試験法の確立

  加藤浩介, 辻野義雄, 佐藤あやの

  日本生物工学会西日本支部大会2020（第5回講演会）, 2020


• Visualization of Physiological Responses Using Membrane Dynamics of Cell-Sized Liposome

  M. Takagi, N. Shimokawa, Y. Tsujino

  IFSCC (International Federation of Societies of Cosmetic Chemists) Congress 2020 Yokohama, 2020


• 細胞模倣膜を用いた界面活性剤刺激性評価に関する研究

  藤根志帆, 佐々木陽介, 下川直史, 辻野義雄, 高木昌宏

  第71回日本生物工学会大会, Sep. 2019


• 細胞模倣膜ダイナミクスに基づく界面活性剤の分子構造と刺激性の相関性

  藤根志帆, 佐々木陽介, 辻野義雄, 下川直史, 高木昌宏

  第13回バイオ関連化学シンポジウム, 2019


• 生体模倣膜ダイナミクスにおける界面活性剤の親水性・疎水性バランスの影響

  佐々木陽介, 辻野義雄, 下川直史, 高木昌宏

  日本化学会第99春季年会, Mar. 2019


• Dynamics of biomimetic membranes induced by low irritating surafactants

  M. Takagi, N. Shimokawa, Y. Tsujino

  IFSCC (International Federation of Societies of Cosmetic Chemist) Congress 2019 Milan, 2019


• 細胞模倣膜ダイナミクス解析に基づく界面活性剤の分子構造と刺激性の相関性

  藤根志帆, 佐々木陽介, 下川直史, 辻野義雄, 高木昌宏

  日本化学会令和元年度北陸地区講演会と研究発表会, 2019


• 分子構造が似た界面活性剤が引き起こす生体模倣膜の変形ダイナミクス

  佐々木陽介, 辻野義雄, 下川直史, 高木昌宏

  日本化学会平成30年度北陸地区講演会と研究発表会, Nov. 2018


• 紫外線ストレス状態モデル膜と低刺激性界面活性剤の相互作用

  高橋祐菜, 辻野義雄, 下川直史, 高木昌宏

  日本化学会平成30年度北陸地区講演会と研究発表会, Nov. 2018


• 生体模倣膜と低刺激性界面活性剤の相互作用

  佐々木陽介, 辻野義雄, 下川直史, 高木昌宏

  日本化学会 第12回バイオ関連化学シンポジウム, Sep. 2018


• ストレス状態モデル膜と低刺激性界面活性剤の相互作用

  高橋祐菜, 辻野義雄, 下川直史, 高木昌宏

  第70回日本生物工学会大会, Sep. 2018


• 分子構造が似た界面活性剤が引き起こす生体模倣膜の変形ダイナミクス

  佐々木陽介, 辻野義雄, 下川直史, 高木昌宏

  第70回日本生物工学会大会, 2018


• Chirality-dependent Interaction of d- and l-menthol with biomembrane models

  M. Takagi, N. Shimokawa, P. Gussan, S. Ohki, K. Hoshino, Y. Tsujino

  IFSCC (International Federation of Societies of Cosmetic Chemists) Congress 2018 Munich, 2018


• Evaluation for skin irritation based on surfactant-induced membrane dynamics

  M. Takagi, S. Shimokawa, N. Shimokawa, Y. Tsujino

  IFSCC (The International Federation of Societies of Cosmetic Chemists) 2016 Congress, Orlando-Florida, 2016


• Difference in membrane dynamics induced by d- and l-methol

  P. Gussan, K. Hoshino, Y. Tsujino, M. Takagi

  57th International Conference on the Bioscience of Lipids, Chamonix- Mont Blanc, France, 2016


• Membrane fluidity and cell signal transduction: Effect of menthol on cell membrane

  M.Takagi, S. Yabuuchi, N. Shimokawa, K. Hoshino, Y. Tsujino

  2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015), Honolulu, 2015


• Effects of a newly found active ingredient, Fraglidel, in Zhenjiang vinegar, on a model mouse of dietary obesity

  Y. Tsujino, M. Takagi, Y. Yoshida, N. Gotoh, A. Satoh

  2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015), Honolulu, 2015


• 8年熟成香醋に認められた抗肥満活性成分：フレグライド１

  辻野義雄, 高木昌宏, 後藤直宏

  第67回日本生物工学会大会, 2015


• 中国伝統調味料「香醋」の有効成分を発見 ～有効成分”Fraglide-1”の効果について～

  辻野義雄

  平成27年北陸ライフケアセミナー, 2015

  [Invited]


• シャンプー製剤によるコンディショニング機能とその頭皮への影響

  辻野義雄

  日本毛髪技術協会 第56回全国セミナー, 2015

  [Invited]


• 最新コスメティックサイエンスと化粧品業界の企業動向

  辻野義雄

  化学生命工学特別セミナー, 2014

  [Invited]


• 天然の黒色色素であるメラニン、その前駆体の酸化酵素誘導による発色反応の意義

  辻野義雄

  健康工学研究部門セミナー, 2013

• 日本農芸化学会

  Mar. 2024 - Present


• 毛髪科学技術者協会

  - Present


• 日本化粧医療学会

  - Present


• 日本分析化学会

  - Present


• 日本動物実験法学会

  - Present


• 日本油化学会

  - Present


• 日本化学会

  - Present


• 日本生物工学会

  - Present


• 日本化粧品技術者会

  - Present

• 化粧品産業の日本と他国の官民連携の違いについて

  ラジオ関西, 高橋真也の令和水戸黄門ラジオ漫遊記, Mar. 2025

  Media report


• 中国伝統の香醋成分フレグライド-1に アトピー性皮膚炎の治療ターゲットとしての可能性

  Feb. 2025, 神戸大学

  Others


• 梅雨の髪悩み解消！?マル秘新成分

  MBS毎日放送, よんチャンTV, 26 Jun. 2024

  Media report


• 最新！くせ毛・縮毛対策

  CBSテレビ, ゴゴスマ～GO,GO Smile！, 25 Jun. 2024

  Media report


• 最新！くせ毛・縮毛対策

  TBSテレビ, THE TIME,, 25 Jun. 2024, マーケティング部

  Media report


• 原発の処理水放出について中国における風評被害の、日本の化粧品メーカーが受ける影響

  ラジオ関西, 高橋真也の令和水戸黄門ラジオ漫遊記, Aug. 2023, 第174回

  Media report


• マイクロバブルシャワーシステム“BUBLISS®”を 共同開発

  神戸大学, 12 Oct. 2022

  Others


• エイジングケア化粧品、神戸大と3企業が共同開発へ「健康に美しく」

  神戸新聞, 12 Apr. 2021

  Paper

• 第６回日本化粧医療学会学術総会

  第６回日本化粧医療学会学術総会

  辻野義雄

  01 Feb. 2025 - 01 Feb. 2025

  Academic society etc


• ㈱資生堂 江連 智暢フェロー特別講演会「顔の老化のメカニズム ～スキンケア研究の最先端～」

  ㈱資生堂 江連 智暢フェロー特別講演会「顔の老化のメカニズム ～スキンケア研究の最先端～」

  辻野義雄

  30 Nov. 2023 - 30 Nov. 2023

  Academic society etc