SEARCH
検索詳細
荻野 千秋大学院工学研究科 応用化学専攻教授
研究活動情報
■ 受賞- 2021年10月 神戸大学, 第13回学長表彰（財務貢献者）
- 2019年10月 神戸大学, 第11回学長表彰（財務貢献者）
- 2018年10月 神戸大学, 第10回学長表彰（財務貢献者）
- 2017年10月 神戸大学, 第9回学長表彰（財務貢献者）
- 2013年09月 日本生物工学会, 生物工学論文賞, 合成代謝経路と細胞表層工学を利用した組換え大腸菌によるセロビオースからのイソプロパノール生産日本国国内学会・会議・シンポジウム等の賞
- 2007年03月 化学工学会, 平成18年度化学工学会 奨励賞(實吉雅郎記念賞), リン脂質修飾酵素ホスホリパーゼＤに関する基礎解析および生産技術の開発
- 2024年08月, Biochemical Engineering Journal[査読有り]研究論文（学術雑誌）
- 2024年06月, Engineering in Life Sciences[査読有り]研究論文（学術雑誌）
- Previously, we synthesized an evolved version of a bio-based polylactide (PLA) on microbial platforms using our engineered lactate-polymerizing enzyme (LPE). This lactate (LA)-based copolyester, LAHB, has advantages over PLA, including improved flexibility and biodegradability, and its properties can be regulated through the LA fraction. To expand the LA-incorporation capacity and improve polymer properties, in the state of in vivo LAHB production, propionyl-CoA transferases (PCTs) that exhibited enhanced production of LA-CoA than the conventional PCTs were selected. Here, the present study has demonstrated that the LA fraction of LAHB could be altered using various PCTs. Enhanced PCT performance was achieved by balancing polymer production and cell growth. Both events are governed by the use of acetyl-CoA, a commonly shared key metabolite. This could be attributed to the different reactivities of individual PCTs towards acetyl-CoA, which serves both as a CoA donor and a leading compound in the TCA cycle. Interestingly, we found complete sequence randomness in the LAHB copolymers, independent of the LA fraction. The mechanism of LA fraction-independent sequence randomness is discussed. This new PCT-based strategy synergistically combines with the evolution of LPE to advance the LAHB project, and enables us to perform advanced applications other than LAHB production utilizing CoA-linked substrates.2024年06月, International journal of biological macromolecules, 133055 - 133055, 英語, 国際誌研究論文（学術雑誌）
- Only a few reports available about the assimilation of hydrophobic or oil-based feedstock as carbon sources by Lipomyces starkeyi. In this study, the ability of L. starkeyi to efficiently utilize free fatty acids (FFAs) and real biomass like palm acid oil (PAO) as well as crude palm kernel oil (CPKO) for growth and lipid production was investigated. PAO, CPKO, and FFAs were evaluated as sole carbon sources or in the mixed medium containing glucose. L. starkeyi was able to grow on the medium supplemented with PAO and FFAs, which contained long-chain length FAs and accumulated lipids up to 35% (w/w) of its dry cell weight. The highest lipid content and lipid concentration were achieved at 50% (w/w) and 10.1 g/L, respectively, when L. starkeyi was cultured in nitrogen-limited mineral medium (-NMM) supplemented with PAO emulsion. Hydrophobic substrate like PAO could be served as promising carbon source for L. starkeyi.2024年05月, Journal of bioscience and bioengineering, 英語, 国内誌研究論文（学術雑誌）
- While flocculation has demonstrated its efficacy in enhancing yeast robustness and ethanol production, its potential application for lactic acid fermentation remains largely unexplored. Our study examined the differences between flocculating and nonflocculating Saccharomyces cerevisiae strains in terms of their metabolic dynamics when incorporating an exogenous lactic acid pathway, across varying cell densities and in the presence of lignocellulose-derived byproducts. Comparative gene expression profiles revealed that cultivating a nonflocculant strain at higher cell density yielded a substantial upregulation of genes associated with glycolysis, energy metabolism, and other key pathways, resulting in elevated levels of fermentation products. Meanwhile, the flocculating strain displayed an inherent ability to sustain high glycolytic activity regardless of the cell density. Moreover, our investigation revealed a significant reduction in glycolytic activity under chemical stress, potentially attributable to diminished ATP supply during the energy investment phase. Conversely, the formation of flocs in the flocculating strain conferred protection against toxic chemicals present in the medium, fostering more stable lactic acid production levels. Additionally, the distinct flocculation traits observed between the two examined strains may be attributed to variations in the nucleotide sequences of the flocculin genes and their regulators. This study uncovers the potential of flocculation for enhanced lactic acid production in yeast, offering insights into metabolic mechanisms and potential gene targets for strain improvement.2024年04月, Yeast (Chichester, England), 41(4) (4), 192 - 206, 英語, 国際誌研究論文（学術雑誌）
- Itaconic acid is a promising biochemical building block that can be used in polymer synthesis. Itaconic acid is currently produced in industry by the natural producer fungus Aspergillus terreus using glucose as a main carbon source. Most research for itaconic acid production using lignocellulosic-based carbon sources was carried out by A. terreus. Engineered Corynebacterium glutamicum strain which can grow in presence of fermentation inhibitors without effect on growth, was used for production of itaconic acid using sweet sorghum juice and bagasse sugar lysate (BSL). BSL contains many inhibitors unlike sorghum juice. C. glutamicum could grow in the media containing both types of lignocellulose-based carbon sources without showing any growth inhibition, however, sorghum juice was better in itaconic acid production than BSL. Different constructed strains of C. glutamicum were used for itaconic acid production, however, C. glutamicum ATCC 13032 pCH-Tad1optAdi1opt strain expressing Adi1/Tad1 genes (trans-pathway) from Ustilago maydis proved to be better in itaconic acid production giving final titer of 8.4 and 4.02 g/L using sweet sorghum juice and BSL as the sole carbon sources by fed-batch fermentation. Our study is the first for production of itaconic acid using sweet sorghum juice and BSL. The present study also proved that C. glutamicum can be used for enhancing itaconic acid production using lignocellulosic-based carbon sources.2024年01月, Enzyme and microbial technology, 172, 110345 - 110345, 英語, 国際誌研究論文（学術雑誌）
- The effect of the membrane separation process to concentrate the xylanase was tested to restrict cellulase dosage on the lignocellulosic waste. Xylanase produced from Streptomyces lividans expressing an endo-xylanase gene from Kitasatospora sp. was concentrated by nanofiltration membrane NTR-7410. This resulted in a seven-fold increase in xylanase activity without changing its specific activity. The saccharification of dilute acid-pretreated empty fruit bunch (EFB) was performed by applying membrane-concentrated xylanase compared with its un-concentrated to commercial cellulase loaded at one filter paper unit/g-biomass. Both glucose and xylose yields obtained by using concentrated xylanase (19.49% and 41.51%, respectively) were higher compared to un-concentrated xylanase (11.71% and 28.71%, respectively). Xylose-assimilating Saccharomyces cerevisiae yeast at initial of 0.5 g/L was then inoculated into the sugar solutions. The yeast had increased ethanol titer and productivity from sugars obtained by using membrane-concentrated xylanase (7.13±0.60 g/L and 0.59±0.06 g/L/h, respectively), compared by using un-concentrated xylanase (4.56±0.43 g/L and 0.38±0.02 g/L/h, respectively).2023年12月, AIP Conference Proceedings, 2972(1) (1)研究論文（国際会議プロシーディングス）
- Abstract Cellulose has been developed as an alternative to petrochemical materials. By comparison with refined nanofibers (RCNFs), lignocellulose nanofibers (LCNFs) show particular promise because it is produced from biomass using only mild pretreatment. The mechanical properties of LCNFs depend on the contained lignin. However, the microscopic location of the lignin contained in LCNFs has not been determined. Thus, we developed two methods to detect and visualize lignin. One uses a scanning transmission electron microscope (STEM) equipped with an energy dispersive X-ray spectroscopy detector. The other method uses an atomic force microscope (AFM) equipped with a cantilever coated with an aromatic molecule. Both methods revealed that the lignin in LCNFs covers a thin cellulose fiber and is precipitated in a grained structure. In particular, the AFM system was able to determine the nanoscopic location of lignin-rich areas. The present study establishes a strong tool for analyzing the characteristics of lignin-containing materials. Graphical abstractSpringer Science and Business Media LLC, 2023年11月, Cellulose, 30(18) (18), 11357 - 11367研究論文（学術雑誌）
- 2023年11月, Biochemical Engineering Journal[査読有り]研究論文（学術雑誌）
- Itaconic acid (IA) is a value-added chemical currently produced by Aspergillus terreus from edible glucose and starch but not from inedible lignocellulosic biomass owing to the high sensitivity to fermentation inhibitors present in the hydrolysate of lignocellulosic biomass. To produce IA from lignocellulosic biomass, a gram-positive bacterium, Corynebacterium glutamicum, with a high tolerance to fermentation inhibitors was metabolically engineered to express a fusion protein composed of cis-aconitate decarboxylase from A. terreus responsible for IA formation from cis-aconitate and a maltose-binding protein (malE) from Escherichia coli. The codon-optimized cadA_malE gene was expressed in C. glutamicum ATCC 13032, and the resulting recombinant strain produced IA from glucose. IA concentration increased 4.7-fold by the deletion of the ldh gene encoding lactate dehydrogenase. With the Δldh strain HKC2029, an 18-fold higher IA production was observed from enzymatic hydrolysate of kraft pulp as a model lignocellulosic biomass than from glucose (6.15 and 0.34 g/L, respectively). The enzymatic hydrolysate of kraft pulp contained various potential fermentation inhibitors involved in furan aldehydes, benzaldehydes, benzoic acids, cinnamic acid derivatives, and aliphatic acid. Whereas cinnamic acid derivatives severely inhibited IA production, furan aldehydes, benzoic acids, and aliphatic acid improved IA production at low concentrations. The present study suggests that lignocellulosic hydrolysate contains various potential fermentation inhibitors; however, some of them can serve as enhancers for microbial fermentation likely due to the changing of redox balance in the cell.2023年07月, Journal of bioscience and bioengineering, 136(1) (1), 7 - 12, 英語, 国内誌研究論文（学術雑誌）
- Itaconic acid (IA), a C5-dicarboxylic acid, is a potential bio-based building block for the polymer industry. There are three pathways for IA production from natural IA producers; however, most of the engineered strains were used for IA production by heterologous expression of cis-aconitate decarboxylase gene (cadA) from Aspergillus terreus. In this study, IA was produced by an engineered Corynebacterium glutamicum ATCC 13032 expressing two different types of genes from two distinct pathways. The first involves the mammalian immunoresponsive gene1 (Irg1) derived from Mus musculus. The second (termed here the trans-pathway) involves two genes from the natural IA producer Ustilago maydis which are aconitate-delta-isomerase (Adi1) and trans-aconitate decarboxylase (Tad1) genes. The constructed strains developing the two distinct IA production pathways: C. glutamicum ATCC 13032 pCH-Irg1opt and C. glutamicum ATCC 13032 pCH-Tad1optadi1opt were used for production of IA from different carbon sources. The results reflect the possibility for IA production from C. glutamicum expressing the trans-pathway (Adi1/Tad1 genes) and cis-pathway (Irg1 gene) other than the well-known cis-pathway that depends mainly on cadA gene from A. terreus. The developed strain expressing trans-pathway from U. maydis; however, proved to be better at IA production with high titers of 12.25, 11.34, and 11.02 g/L, and a molar yield of 0.22, 0.42, and 0.43 mol/mol from glucose, maltose, and sucrose, respectively, via fed-batch fermentation. The present study suggests that trans-pathway is better than cis-pathway for IA production in engineered C. glutamicum.2023年06月, Journal of bioscience and bioengineering, 136(2) (2), 109 - 116, 英語, 国内誌研究論文（学術雑誌）
- Plants produce sugars by photosynthesis and use them for growth and development. Sugars are transported from source-to-sink organs via the phloem in the vasculature. It is well known that vascular development is precisely controlled by plant hormones and peptide hormones. However, the role of sugars in the regulation of vascular development is poorly understood. In this study, we examined the effects of sugars on vascular cell differentiation using a vascular cell induction system named Vascular cell Induction culture System Using Arabidopsis Leaves (VISUAL). We found that sucrose has the strongest inhibitory effect on xylem differentiation among several types of sugars. Transcriptome analysis revealed that sucrose suppresses xylem and phloem differentiation from cambial cells. Physiological and genetic analysis suggested that sucrose might function through the BES1 transcription factor, which is the central regulator of vascular cell differentiation. Conditional overexpression of cytosolic invertase led to a decrease in the number of cambium layers due to an imbalance between cell division and differentiation. Taken together, our results suggest that sucrose potentially acts as a signal that integrates environmental conditions with the developmental program.2023年05月, Plant & cell physiology, 64(12) (12), 1511 - 1522, 英語, 国内誌研究論文（学術雑誌）
- Sorghum is the fifth most important cereal crop. Here we performed molecular genetic analyses of the ‘SUGARY FETERITA’ (SUF) variety, which shows typical sugary endosperm traits (e.g., wrinkled seeds, accumulation of soluble sugars, and distorted starch). Positional mapping indicated that the corresponding gene was located on the long arm of chromosome 7. Within the candidate region of 3.4 Mb, a sorghum ortholog for maize Su1 (SbSu) encoding a starch debranching enzyme ISA1 was found. Sequencing analysis of SbSu in SUF uncovered nonsynonymous single nucleotide polymorphisms (SNPs) in the coding region, containing substitutions of highly conserved amino acids. Complementation of the rice sugary-1 (osisa1) mutant line with the SbSu gene recovered the sugary endosperm phenotype. Additionally, analyzing mutants obtained from an EMS-induced mutant panel revealed novel alleles with phenotypes showing less severe wrinkles and higher Brix scores. These results suggested that SbSu was the corresponding gene for the sugary endosperm. Expression profiles of starch synthesis genes during the grain-filling stage demonstrated that a loss-of-function of SbSu affects the expression of most starch synthesis genes and revealed the fine-tuned gene regulation in the starch synthetic pathway in sorghum. Haplotype analysis using 187 diverse accessions from a sorghum panel revealed the haplotype of SUF showing severe phenotype had not been used among the landraces and modern varieties. Thus, weak alleles (showing sweet and less severe wrinkles), such as in the abovementioned EMS-induced mutants, are more valuable for grain sorghum breeding. Our study suggests that more moderate alleles (e.g. produced by genome editing) should be beneficial for improving grain sorghum.Frontiers Media SA, 2023年02月, Frontiers in Plant Science, 14, 1114935 - 1114935, 英語, 国際誌研究論文（学術雑誌）
- Lignocellulose is resistant to degradation and requires pretreatment before hydrolytic enzymes can release fermentable sugars. Sulfuric acid has been widely used for biomass pretreatment, but high amount of degradation products usually occurred when using this method. To enhance accessibility to cellulose, we studied the performances of several dilute organic acid pretreatments of sugarcane bagasse and oil palm empty fruit bunch fiber. The results revealed that pretreatment with maleic acid yields the highest xylose and glucose release among other organic acids. The effects of concentration, duration of heating and heating temperature were further studied. Dilute maleic acid 1% (w/w) pretreatment at 180 °C was the key to its viability as a substitute for sulfuric acid. Moreover, maleic acid did not seem to highly promote the formation of either furfural or 5-HMF in the liquid hydrolysate after pretreatment.Elsevier BV, 2023年02月, Bioresource Technology, 369, 128382 - 128382, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Inorganic nanoparticles with multiple functions have been attracting attention as multimodal nanoprobes in bioimaging, biomolecule detection, and medical diagnosis and treatment. A drawback of conventional metallic nanoparticle-based nanoprobes is the Ohmic losses that lead to fluorescence quenching of attached molecules and local heating under light irradiation. Here, metal-free nanoprobes capable of scattering/fluorescence dual-mode imaging are developed. The nanoprobes are composed of a silicon nanosphere core having efficient Mie scattering in the visible to near infrared range and a fluorophore doped silica shell. The dark-field scattering and photoluminescence images/spectra for nanoprobes made from different size silicon nanospheres and different kinds of fluorophores are studied by single particle spectroscopy. The fluorescence spectra are strongly modified by the Mie modes of a silicon nanosphere core. By comparing scattering and fluorescence spectra and calculated Purcell factors, the fluorescence enhancement factor is quantitatively discussed. In vitro scattering/fluorescence imaging studies on human cancer cells demonstrate that the developed nanoparticles work as scattering/fluorescence dual-mode imaging nanoprobes.Wiley, 2023年01月, Small, 19(14) (14), 2207318 - 2207318, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- 4-Aminophenylalanine as a diamine monomer to synthesize unique biopolyimides was produced from lignocellulosic biomass by microbial fermentation. Fermentation inhibitors present in lignocellulosic hydrolysate of sorghum bagasse were identified.Royal Society of Chemistry (RSC), 2023年, RSC Sustainability研究論文（学術雑誌）
- Abstract The bottleneck for the production of biofuels from microalgae consists on costly harvesting processes and low lipid production, immobilization technology could play a part on making the production of biofuels more feasible. The aim of this study was to evaluate the effect of alginate immobilization on the growth and lipid productivity of the microalgae Chlorella sorokiniana, so far, the main focus of immobilization technology has been its use for wastewater treatment and nutrient removal from effluents. The microalgae Chlorella sorokiniana was cultured in both free and immobilized forms under optimal autotrophic growth conditions. Microalgae were immobilized in calcium alginate beads generated by mixing algal cells with a sodium alginate solution, followed by extrusion into a CaCl2 solution. The results obtained in this study showed that the growth of the microalgae immobilized in alginate beads, was enhanced and achieved a dry cell weight 1.4-fold higher than that of a free cell culture, a higher light transmittance was also achieved in the alginate immobilized culture, and the lipid productivity was increased from 54.21 ± 2.48 mg l−1 d in the free cell culture to 82.22 ± 8.48 mg l−1 d in the immobilized culture. These results demonstrate the effectiveness of immobilization technology for promoting growth and lipid productivity in the microalgae Chlorella sorokiniana.IOP Publishing, 2023年01月, Journal of Physics: Energy, 5(1) (1), 014019 - 014019, 英語[査読有り]研究論文（学術雑誌）
- Elsevier BV, 2022年12月, Biochemical Engineering Journal, 188, 108681 - 108681, 英語[査読有り]研究論文（学術雑誌）
- New enzyme functions exist within the increasing number of unannotated protein sequences. Novel enzyme discovery is necessary to expand the pathways that can be accessed by metabolic engineering for the biosynthesis of functional compounds. Accordingly, various machine learning models have been developed to predict enzymatic reactions. However, the ability to predict unknown reactions that are not included in the training data has not been clarified. In order to cover uncertain and unknown reactions, a wider range of reaction types must be demonstrated by the models. Here, we establish 16 expanded enzymatic reaction prediction models developed using various machine learning algorithms, including deep neural network. Improvements in prediction performances over that of our previous study indicate that the updated methods are more effective for the prediction of enzymatic reactions. Overall, the deep neural network model trained with combined substrate-enzyme-product information exhibits the highest prediction accuracy with Macro F1 scores up to 0.966 and with robust prediction of unknown enzymatic reactions that are not included in the training data. This model can predict more extensive enzymatic reactions in comparison to previously reported models. This study will facilitate the discovery of new enzymes for the production of useful substances.2022年09月, Journal of Physical Chemistry B, 126(36) (36), 6762 - 6770, 英語, 国際誌研究論文（学術雑誌）
- New enzyme functions exist within the increasing number of unannotated protein sequences. Novel enzyme discovery is necessary to expand the pathways that can be accessed by metabolic engineering for the biosynthesis of functional compounds. Accordingly, various machine learning models have been developed to predict enzymatic reactions. However, the ability to predict unknown reactions that are not included in the training data has not been clarified. In order to cover uncertain and unknown reactions, a wider range of reaction types must be demonstrated by the models. Here, we establish 16 expanded enzymatic reaction prediction models developed using various machine learning algorithms, including deep neural network. Improvements in prediction performances over that of our previous study indicate that the updated methods are more effective for the prediction of enzymatic reactions. Overall, the deep neural network model trained with combined substrate-enzyme-product information exhibits the highest prediction accuracy with Macro F1 scores up to 0.966 and with robust prediction of unknown enzymatic reactions that are not included in the training data. This model can predict more extensive enzymatic reactions in comparison to previously reported models. This study will facilitate the discovery of new enzymes for the production of useful substances.2022年09月, The journal of physical chemistry. B, 126(36) (36), 6762 - 6770, 英語, 国際誌研究論文（学術雑誌）
- Acidic and chemical inhibitor stresses undermine efficient lactic acid bioproduction from lignocellulosic feedstock. Requisite coping treatments, such as detoxification and neutralizing agent supplementation, can be eliminated if a strong microbial host is employed in the process. Here, we exploited an originally robust yeast, Saccharomyces cerevisiae BTCC3, as a production platform for lactic acid. This wild-type strain exhibited a rapid cell growth in the presence of various chemical inhibitors compared to laboratory and industrial strains, namely BY4741 and Ethanol-red. Pathway engineering was performed on the strain by introducing an exogenous LDH gene after disrupting the PDC1 and PDC5 genes. Facilitated by this engineered strain, high cell density cultivation could generate lactic acid with productivity at 4.80 and 3.68 g L-1 h-1 under semi-neutralized and non-neutralized conditions, respectively. Those values were relatively higher compared to other studies. Cultivation using real lignocellulosic hydrolysate was conducted to assess the performance of this engineered strain. Non-neutralized fermentation using non-detoxified hydrolysate from sugarcane bagasse as a medium could produce lactic acid at 1.69 g L-1 h-1, which was competitive to the results from other reports that still included detoxification and neutralization steps in their experiments. This strategy could make the overall lactic acid bioproduction process simpler, greener, and more cost-efficient.Springer Science and Business Media LLC, 2022年08月, Scientific reports, 12(1) (1), 13645 - 13645, 英語, 国際誌研究論文（学術雑誌）
- When lignocellulosic biomass is utilized as a fermentative substrate to produce biochemicals, the existence of a yeast strain resistant to inhibitory chemical compounds (ICCs) released from the biomass becomes critical. To achieve the purpose, in this study, Saccharomyces yeast strains from a NBRC yeast culture collection were used for exploration and evaluated in two different media containing ICCs that mimic one another but resemble the hydrolysate of real biomass. Among them, S. cerevisiae F118 strain shows robustness upon the fermentation with unique flocculation trait that was strongly responsive to ICC stress. When this strain was cultured in the presence of ICCs, its cell wall hydrophobicity increased dramatically, and reduced significantly when the ICCs were depleted, demonstrating that cell-surface hydrophobicity can also act as an adaptive response to the ICCs. Cells from the strain with the highest cell-wall hydrophobicity displayed progressively stronger flocculation, indicating that the F118 strain is having unique robustness under ICC stress. Gene expression perturbation analysis revealed that mot3 gene encoding regulatory Mot3p from the F118 strain was expressed in response to the concentration of ICCs. This gene was found to control expression of ygp1 gene that encoding Ygp1p, one of cell wall proteins. Deep sequencing analysis revealed that the Mot3p of the F118 strain features a unique insertion and deletion of nucleotides that encode glutamine or asparagine residues, particularly in N-terminal domain, as determined by comparison to the Mot3p sequence from the S288c strain, which was employed as a control strain. Furthermore, the cell wall hydrophobicity of the S288c strain was greatly enhanced and became ICC-responsive after gene swapping with the mot3 gene from the F118 strain. The gene-swapped S288c strain fermented 6-fold faster than the wild-type strain, producing 14.5 g/L of ethanol from 30 g/L of glucose consumed within 24 h in a medium containing the ICCs. These such modifications to Mot3p in unique locations in its sequence have a potential to change the expression of a gene involved in cell wall hydrophobicity and boosted the flocculation response to ICC stress, allowing for the acquisition of extraordinary robustness.Elsevier BV, 2022年07月, Metabolic Engineering, 72, 82 - 96, 英語, 国際誌研究論文（学術雑誌）
- Elsevier BV, 2022年06月, Journal of Environmental Chemical Engineering, 10(3) (3), 107550 - 107550, 英語[査読有り]研究論文（学術雑誌）
- Background Despite recent advances in radiotherapy, radioresistance in patients with pancreatic cancer remains a crucial dilemma for clinical treatment. Cancer stem cells (CSCs) represent a major factor in radioresistance. Developing a potent radiosensitizer may be a novel candidate for the eradication of pancreatic CSCs. Methods CSCs were isolated from MIA PaCa-2 and PANC1 human pancreatic cancer cell lines. Titanium peroxide nanoparticles (TiOxNPs) were synthesized from titanium dioxide nanoparticles (TiO(2)NPs) and utilized as radiosensitizers when added one hour prior to radiation exposure. The antitumor activity of this novel therapeutic strategy was evaluated against well-established pancreatic CSCs model both in vitro and in vivo. Results It is shown that TiOxNPs combined with ionizing radiation exhibit anti-cancer effects on radioresistant CSCs both in vitro and in vivo. TiOxNPs exhibited a synergistic effect with radiation on pancreatic CSC-enriched spheres by downregulating self-renewal regulatory factors and CSC surface markers. Moreover, combined treatment suppressed epithelial-mesenchymal transition, migration, and invasion properties in primary and aggressive pancreatic cancer cells by reducing the expression of proteins relevant to these processes. Notably, radiosensitizing TiOxNPs suppressed the growth of pancreatic xenografts following primary or dissociating sphere MIA PaCa-2 cell implantation. It is inferred that synergy is formed by generating intolerable levels of reactive oxygen species (ROS) and inactivating the AKT signaling pathway. Conclusions Our data suggested the use of TiOxNPs in combination with radiation may be considered an attractive therapeutic strategy to eliminate pancreatic CSCs.BMC, 2022年04月, JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 41(1) (1), 146 - 146, 英語, 国際誌研究論文（学術雑誌）
- A novel endo-beta-1,4-mannanase gene was cloned from a novel actinomycetes, Nonomuraea jabiensis ID06-379, isolated from soil, overexpressed as an extracellular protein (47.8 kDa) in Streptomyces lividans 1326. This new endo-1,4-beta-mannanase gene (manNj6-379) is encoded by 445-amino acids. The ManNj6-379 consists of a 28-residue signal peptide and a carbohydrate-binding module of family 2 belonging to the glycoside hydrolase (GH) family 5, with 59-77% identity to GH5 mannan endo-1,4-beta-mannanase. The recombinant ManNj6-379 displayed an optimal pH of 6.5 with pH stability ranging between 5.5 and 7.0 and was stable for 120 min at 50 & DEG;C and lower temperatures. The optimal temperature for activity was 70 & DEG;C. An enzymatic hydrolysis assay revealed that ManNj6-379 could hydrolyze commercial beta-mannan and biomass containing mannan.MDPI, 2022年02月, PROCESSES, 10(2) (2), 英語研究論文（学術雑誌）
- The aromatic compound 3-amino-4-hydroxybenzoic acid (3,4-AHBA) can be employed as a raw material for high-performance industrial plastics. The aim of this study is to produce 3,4-AHBA via a recombinant Streptomyces lividans strain containing griI and griH genes derived from Streptomyces griseus using culture medium with glucose and/or xylose, which are the main components in lignocellulosic biomass. Production of 3,4-AHBA by the recombinant S. lividans strain was successful, and the productivity was affected by the kind of sugar used as an additional carbon source. Metabolic profiles revealed that L aspartate-4-semialdehyde (ASA), a precursor of 3,4-AHBA, and coenzyme NADPH were supplied in greater amounts in xylose medium than in glucose medium. Moreover, cultivation in TSB medium with a mixed sugar (glucose/xylose) was found to be effective for 3,4-AHBA production, and optimal conditions for efficient production were designed by changing the ratio of glucose to xylose. The best productivity of 2.70 g/L was achieved using a sugar mixture of 25 g/L glucose and 25 g/L xylose, which was 1.5 times higher than the result using 50 g/L glucose alone. These results suggest that Streptomyces is a suitable candidate platform for 3,4-AHBA production from lignocellulosic biomass-derived sugars under appropriate culture conditions.Microbiology Research Foundation, 2022年, The Journal of General and Applied Microbiology, 68(2) (2), 109 - 116, 英語, 国内誌[査読有り]研究論文（学術雑誌）
- Each year, the palm oil industry generates a significant amount of biomass residue and effluent waste; both have been identified as significant sources of greenhouse gas (GHG) emissions. This issue poses a severe environmental challenge for the industry due to the possibility of long-term negative effects on human well-being. The palm-oil industry must invest significantly in the technology that is required to resolve these issues and to increase the industry's sustainability. However, current technologies for converting wastes such as lignocellulosic components and effluents into biochemical products are insufficient for optimal utilization. This review discusses the geographical availability of palm-oil biomass, its current utilization routes, and then recommends the development of technology for converting palm-oil biomass into value-added products through an integrated biorefinery strategy. Additionally, this review summarizes the palm oil industry's contribution to achieving sustainable development goals (SDGs) through a circular bioeconomy concept.Elsevier BV, 2022年01月, Bioresource Technology, 344(Pt B) (Pt B), 126266 - 126266, 英語, 国際誌研究論文（学術雑誌）
- Lignocellulosic biomass has great potential as an inedible feedstock for bioplastic synthesis, although its use is still limited compared to current edible feedstocks of glucose and starch. This review focuses on recent advances in the production of biopolymers and biomonomers from lignocellulosic feedstocks with downstream processing and chemical polymer syntheses. In microbial production, four routes composed of existing poly (lactic acid) and polyhydroxyalkanoates (PHAs) and the emerging biomonomers of itaconic acid and aromatic compounds were presented to review present challenges and future perspectives, focusing on the use of lignocellulosic feedstocks. Recently, advances in purification technologies decreased the number of processes and their environmental burden. Additionally, the unique structures and high-performance of emerging lignocellulose-based bioplastics have expanded the possibilities for the use of bioplastics. The sequence of processes provides insight into the emerging technologies that are needed for the practical use of bioplastics made from lignocellulosic biomass.Elsevier BV, 2022年01月, Bioresource Technology, 344(Pt B) (Pt B), 126165 - 126165, 英語, 国際誌研究論文（学術雑誌）
- Springer Science and Business Media LLC, 2021年12月, Microbial Cell Factories, 20(1) (1), 228 - 228, 英語, 国際誌
Abstract Background Bio-based aromatic compounds are of great interest to the industry, as commercial production of aromatic compounds depends exclusively on the unsustainable use of fossil resources or extraction from plant resources. γ-amino acid 3-amino-4-hydroxybenzoic acid (3,4-AHBA) serves as a precursor for thermostable bioplastics.Results Under aerobic conditions, a recombinantCorynebacterium glutamicum strain KT01 expressinggriH andgriI genes derived fromStreptomyces griseus produced 3,4-AHBA with large amounts of amino acids as by-products. The specific productivity of 3,4-AHBA increased with decreasing levels of dissolved oxygen (DO) and was eightfold higher under oxygen limitation (DO = 0 ppm) than under aerobic conditions (DO ≥ 2.6 ppm). Metabolic profiles during 3,4-AHBA production were compared at three different DO levels (0, 2.6, and 5.3 ppm) using the DO-stat method. Results of the metabolome analysis revealed metabolic shifts in both the central metabolic pathway and amino acid metabolism at a DO of < 33% saturated oxygen. Based on this metabolome analysis, metabolic pathways were rationally designed for oxygen limitation. Anldh deletion mutant, with the loss of lactate dehydrogenase, exhibited 3.7-fold higher specific productivity of 3,4-AHBA at DO = 0 ppm as compared to the parent strain KT01 and produced 5.6 g/L 3,4-AHBA in a glucose fed-batch culture.Conclusions Our results revealed changes in the metabolic state in response to DO concentration and provided insights into oxygen supply during fermentation and the rational design of metabolic pathways for improved production of related amino acids and their derivatives.Graphical Abstract 研究論文（学術雑誌） - Recently, a hyphae-dispersed type of filamentous fungus Aspergillus oryzae was constructed via genetic engineering, and industrial applications are expected due to the ease of handling and to the level of protein production properties. In this study, we constructed cellulase-expressing strains using wild-type and hyphae-dispersed strains to investigate the correlation between protein productivity and metabolism. Compared with the original strain, the hyphae-dispersed cellulase-expressing strain showed elevated cellulase activity, rapid glucose consumption, increased mycelial dry weight, an increased expression of cellulase genes, and activated respiration activity. Comparative metabolomic analysis showed fewer metabolites in the glycolysis and TCA cycles in the dispersed strains than in the original strains. These results indicate that the flux of carbohydrate metabolism in the hyphae-dispersed strains is smoother than that in the original strains. Such efficient metabolic flux would contribute to efficient energy conversion and to sufficient energy supply to anabolisms, such as mycelial growth and protein production. Our findings suggest that the hyphae-dispersed strains could be a useful host not only for protein production but also for the biological production of various chemicals such as organic acids.Elsevier BV, 2021年08月, Journal of Bioscience and Bioengineering, 132(2) (2), 140 - 147, 英語, 国内誌研究論文（学術雑誌）
- Wiley, 2021年05月, Biofuels, Bioproducts and Biorefining, 15(3) (3), 804 - 814, 英語研究論文（学術雑誌）
- (一社)日本インターベンショナルラジオロジー学会, 2021年04月, 日本インターベンショナルラジオロジー学会雑誌, 36(Suppl.) (Suppl.), 191 - 191, 日本語ウサギVX2腫瘍モデルを用いた過酸化チタンナノ粒子動注の基礎的研究
- Polyacrylic acid-modified titanium peroxide nanoparticles (PAA-TiOx NPs) are promising radiosensitizers that enhance the therapeutic effect of X-ray irradiation after local injection into tumors. However, the mechanism for this reaction has remained unclear with the exception of the involvement of hydrogen peroxide (H2O2), which is released by PAA-TiOx NPs to a liquid phase during dispersion. In the present study, a clonogenic assay was used to compare PAA-TiOx NPs with free H2O2 molecules to investigate the effect exerted on the radiosensitivity of cancer cells in vitro. A cell-free dialysis method revealed that a portion of the H2O2 adsorbed onto the PAA-TiOx NPs during synthesis could be released during a treatment regimen. The H2O2 release lasted for 7 h, which was sufficient for one radiation treatment procedure. For in vitro experiments, cultured human pancreatic cancer cells took up PAA-TiOx NPs in 10 min after administration. Interestingly, when the cells were washed with a buffer after treatment with either a PAA-TiOx NP or H2O2 solution, the intracellular H2O2 levels remained higher with PAA-TiOx NP treatment compared with the H2O2 solution treatment. Furthermore, the effects of subsequent X-ray irradiation corresponded to the intracellular H2O2 levels. These results indicate that PAA-TiOx NPs are efficient carriers of H2O2 into cancer cells and thus enhance the radiosensitivity.Elsevier BV, 2021年02月, Colloids and Surfaces B: Biointerfaces, 198, 111451 - 111451, 英語, 国際誌研究論文（学術雑誌）
- We describe a novel expression cassette that enables efficient and constitutive expression of the ZZ domain derived from Staphylococcus aureus protein A on the yeast cell surface to easily prepare yeast-based immunosorbents. Using this expression cassette containing the PGK1 promoter, a secretion signal derived from α-factor, and a Flo1-derived anchor protein, we successfully created a yeast-based immunosorbent for human serum albumin.Microbiology Research Foundation, 2021年, The Journal of General and Applied Microbiology, 67(6) (6), 265 - 268, 英語, 国内誌研究論文（学術雑誌）
- Wiley, 2021年01月, Advanced Sustainable Systems, 5(1) (1), 2000193 - 2000193研究論文（学術雑誌）
- Cambridge University Press ({CUP}), 2020年12月, MRS Communications, 10(4) (4), 680 - 686[査読有り]研究論文（学術雑誌）
- Elsevier BV, 2020年12月, Renewable and Sustainable Energy Reviews, 134, 110355 - 110355研究論文（学術雑誌）
- American Chemical Society (ACS), 2020年11月, ACS Applied Bio Materials, 3(11) (11), 7743 - 7751研究論文（学術雑誌）
- Continuous expansion of agriculture for the production of biofuels may be considered a potential source of greenhouse gas (GHG) emissions due to the ever-increasing amount of waste and fossil fuel-dependent materials involved. Agricultural waste utilization through the circular bioeconomy concept offers a pathway to reduce GHG emissions. Palm oil production, for instance, produces palm kernel shells (PKS) and palm oil mill effluents (POME) as wastes in enormous amounts. PKS and POME account for >60% of solid and liquid waste generated from the mill. In this work, the feasibility of a circular palm bioeconomy is explored where waste PKS is directly converted to activated carbons (AC) in a cost-effective one-step technique (550 degrees C, 10 mL min(-1) N-2) that departs from the conventional two-step (carbonization and activation) technique. Characterization of the synthesized carbons, PKAC, using Fourier-transform infrared spectroscopy and scanning electron microscopy with energy-dispersive X-ray spectroscopy showed oxygen-rich morphological features that were 2-fold higher than in bituminous coal-derived AC. The <10 mu m pore diameters of PKAC were relevant in the immobilization of Aspergillus oryzae whole-cells expressing Fusarium heterosporum lipase (PKAC-FHL). For biodiesel production, the 81.6 IU mg(-1) specific activity of PKAC-FHL yielded >= 97.5 wt% fatty acid methyl ester (FAME) from POME. While offering the benefits of environmental remediation through effluent utilization, the produced FAME showed 48.8 Cetane Number and cold-flow properties that conform to ASTM and EN biodiesel requirements. This waste valorization approach, thus, proposes a green framework for the use of POME and PKS in immobilized lipase-catalyzed methanolysis.Elsevier BV, 2020年11月, Biomass and Bioenergy, 142, 105768 - 105768, 英語[査読有り]研究論文（学術雑誌）
- Pyruvate is a central metabolite for the biological production of various chemicals. In eukaryotes, pyruvate produced by glycolysis is used in conversion to ethanol and lactate and in anabolic metabolism in the cytosol, or is transported into the mitochondria for use as a substrate in the tricarboxylic acid (TCA) cycle. In this study, we focused on controlling pyruvate metabolism in aerobic microorganisms for the biological production of various chemicals. We successfully improved productivity by redirecting pyruvate metabolism in the aerobic filamentous fungus Aspergillus oryzae via the deletion of two genes that encode pyruvate decarboxylase and mitochondrial pyruvate carriers. Production of ethanol as a major byproduct was completely inhibited, and the limited translocation of pyruvate into the mitochondria shifted the metabolism from respiration for energy conversion to the effective production of lactate or 2,3-butandiole, even under aerobic conditions. Metabolomic and transcriptomic analyses showed an emphasis on glycolysis and a repressed TCA cycle. Although the dry mycelial weights of the deletion mutants were reduced compared with those of wild type, the titer and yields of the target products were drastically increased. In particular, the redirection of pyruvate metabolism shifted from anabolism for biomass production to catabolism for the production of target chemicals. Conclusively, our results indicate that the redirection of pyruvate metabolism is a useful strategy in the metabolic engineering of aerobic microorganisms.2020年07月, Metabolic engineering, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- The development of potentially safe radiosensitizing agents is essential to enhance the treatment outcomes of radioresistant cancers. The titanium peroxide nanoparticle (TiOxNP) was originally produced using the titanium dioxide nanoparticle, and it showed excellent reactive oxygen species (ROS) generation in response to ionizing radiation. Surface coating the TiOxNPs with polyacrylic acid (PAA) showed low toxicity to the living body and excellent radiosensitizing effect on cancer cells. Herein, we evaluated the mechanism of radiosensitization by PAA-TiOxNPs in comparison with gold nanoparticles (AuNPs) which represent high-atomic-number nanoparticles that show a radiosensitizing effect through the emission of secondary electrons. The anticancer effects of both nanoparticles were compared by induction of apoptosis, colony-forming assay, and the inhibition of tumor growth. PAA-TiOxNPs showed a significantly more radiosensitizing effect than that of AuNPs. A comparison of the types and amounts of ROS generated showed that hydrogen peroxide generation by PAA-TiOxNPs was the major factor that contributed to the nanoparticle radiosensitization. Importantly, PAA-TiOxNPs were generally nontoxic to healthy mice and caused no histological abnormalities in the liver, kidney, lung, and heart tissues.2020年06月, Nanomaterials (Basel, Switzerland), 10(6) (6), 英語, 国際誌研究論文（学術雑誌）
- 2020年05月, Processes, 8(5) (5), 619, 英語High enzymatic recovery and purification of xylooligosaccharides from empty fruit bunch via nanofiltration[査読有り]研究論文（学術雑誌）
- 2020年04月, Processes, 8(4) (4), 450, 英語Concentration of Lipase from Aspergillus oryzae Expressing Fusarium heterosporum by Nanofiltration to Enhance Transesterification[査読有り]
- Unannotated gene sequences in databases are increasing due to sequencing advances. Therefore, computational methods to predict functions of unannotated genes are needed. Moreover, novel enzyme discovery for metabolic engineering applications further encourages annotation of sequences. Here, enzyme functions are predicted using two general approaches, each including several machine learning algorithms. First, Enzyme-models (E-models) predict Enzyme Commission (EC) numbers from amino acid sequence information. Second, Substrate-Enzyme models (SE-models) are built to predict substrates of enzymatic reactions together with EC numbers, and Substrate-Enzyme-Product models (SEP-models) are built to predict substrates, products, and EC numbers. While accuracy of E-models is not optimal, SE-models and SEP-models predict EC numbers and reactions with high accuracy using all tested machine learning-based methods. For example, a single Random Forests-based SEP-model predicts EC first digits with an Average AUC score of over 0.94. Various metrics indicate that the current strategy of combining sequence and chemical structure information is effective at improving enzyme reaction prediction.2020年03月, Journal of chemical information and modeling, 60(3) (3), 1833 - 1843, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Palm oil mill effluent (POME), a liquid waste from the palm oil industry, presents an alternative source for biodiesel production without interfering with food supply. This study attempted to produce biodiesel from untreated POME with aqueous ethanol using Thermomyces lanuginosus lipase as a biocatalyst. The effects of enzyme concentration, alcohol to oil ratio, and ethanol concentration were considered in the transesterification reaction. The optimum conditions were 2100 U lipase loading, 4 : 1 ethanol to oil molar ratio, and 45% (v/v) ethanol concentration at 40 degrees C reaction, and under 24 hours. The maximum fatty acid ethyl ester (FAEE) yield reached 97.43% (w/w) under these conditions. Integration of dilute ethanol for the conversion of POME to biodiesel could be promising as both feedstocks could be obtained from the same location, and thus reduce the logistical burden on biodiesel production.ROYAL SOC CHEMISTRY, 2020年03月, SUSTAINABLE ENERGY & FUELS, 4(3) (3), 1105 - 1111, 英語[査読有り]研究論文（学術雑誌）
- 2020年03月, Fuel Processing Technology, 199, 106278, 英語Biodiesel-mediated biodiesel production: A recombinant Fusarium heterosporum lipase-catalyzed transesterification of crude plant oils[査読有り]研究論文（学術雑誌）
- Microalgae biomass contains various useful bio-active components. Microalgae derived biodiesel has been researched for almost two decades. However, sole biodiesel extraction from microalgae is time-consuming and is not economically feasible due to competitive fossil fuel prices. Microalgae also contains proteins and carbohydrates in abundance. Microalgae are likewise utilized to extract high-value products such as pigments, anti-oxidants and long-chain polyunsaturated fatty acids which are useful in cosmetic, pharmaceutical and nutraceutical industry. These compounds can be extracted simultaneously or sequentially after biodiesel extraction to reduce the total expenditure involved in the process. This approach of bio-refinery is necessary to promote microalgae in the commercial market. Researchers have been keen on utilizing the bio-refinery approach to exploit the valuable components encased by microalgae. Apart from all the beneficial components housed by microalgae, they also help in reducing the anthropogenic CO2 levels of the atmosphere while utilizing saline or wastewater. These benefits enable microalgae as a potential source for bio-refinery approach. Although life-cycle analysis and economic assessment do not favor the use of microalgae biomass feedstock to produce biofuel and co-products with the existing techniques, this review still aims to highlight the beneficial components of microalgae and their importance to humans. In addition, this article also focuses on current and future aspects of improving the feasibility of bio-processing for microalgae bio-refinery.2019年12月, Bioengineered, 10(1) (1), 574 - 592, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- 2019年09月, BIOCHEMICAL ENGINEERING JOURNAL, 149[査読有り]
- 2019年09月, BIOMASS & BIOENERGY, 128[査読有り]
- Xylitol is a highly valuable commodity chemical used extensively in the food and pharmaceutical industries. The production of xylitol from d-xylose involves a costly and polluting catalytic hydrogenation process. Biotechnological production from lignocellulosic biomass by micro-organisms like yeasts is a promising option. In this study, xylitol is produced from lignocellulosic biomass by a recombinant strain of Saccharomyces cerevisiae (S. cerevisiae) (YPH499-SsXR-AaBGL) expressing cytosolic xylose reductase (Scheffersomyces stipitis xylose reductase [SsXR]), along with a β-d-glucosidase (Aspergillus aculeatus β-glucosidase 1 [AaBGL]) displayed on the cell surface. The simultaneous cofermentation of cellobiose/xylose by this strain leads to an ≈2.5-fold increase in Yxylitol/xylose (=0.54) compared to the use of a glucose/xylose mixture as a substrate. Further improvement in the xylose uptake by the cell is achieved by a broad evaluation of several homologous and heterologous transporters. Homologous maltose transporter (ScMAL11) shows the best performance in xylose transport and is used to generate the strain YPH499-XR-ScMAL11-BGL with a significantly improved xylitol production capacity from cellobiose/xylose coutilization. This report constitutes a promising proof of concept to further scale up the biorefinery industrial production of xylitol from lignocellulose by combining cell surface and metabolic engineering in S. cerevisiae.2019年09月, Biotechnology journal, 14(9) (9), e1800704, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- The original version of this Comment contained errors in the legend of Figure 2, in which the locations of the fifteenth and sixteenth GBA members were incorrectly given as '(15) Australian Genome Foundry, Macquarie University; (16) Australian Foundry for Advanced Biomanufacturing, University of Queensland.'. The correct version replaces this with '(15) Australian Foundry for Advanced Biomanufacturing (AusFAB), University of Queensland and (16) Australian Genome Foundry, Macquarie University'. This has been corrected in both the PDF and HTML versions of the Comment.2019年07月, Nature communications, 10(1) (1), 3132 - 3132, 英語, 国際誌[査読有り]
- © 2019 American Chemical Society. An organosolv pretreatment consisting of an H2SO4/n-butanol biphasic system was designed to separate lignocellulosic biomass in three distinct phases: a cellulose-rich solid residue, hydrolyzed hemicelluloses in an aqueous phase, and lignin dissolved in a hydrophobic butanol phase. In the present study, the versatility of the process was investigated on materials of various compositions and origins: sugar cane bagasse, tall fescue, sugar beet pulp, eucalyptus, beech, and Japanese cedar. The efficiency was assessed in terms of lignin removal from the raw biomass and purity of the recovered cellulosic residue using the Klason method as well as improvement on enzymatic saccharification (increased from 18.7% to 96%). Results were correlated to biomass types and composition, and in comparison to an organic solvent-free method (dilute acid). Up to 81% cellulose purity corresponding to 87% lignin removal was achieved. Results were corroborated by scanning electron microscopy showing an absence of lignin deposition. Lignin molecular weight (GPC), structure (2D-HSQC NMR), recovery, and purity (up to 96%) have been investigated. Moreover, organic compounds responsible for fermentation inhibition were partially solubilized in the butanol, decreasing the concentration in the aqueous phase. Efficient butanol pretreatment applied on hardwood, bagasse, and herbaceous matter is promising. However, Japanese cedar (softwood) was too recalcitrant for this process.2019年07月, ACS Sustainable Chemistry and Engineering, 7(13) (13), 11069 - 11079[査読有り]研究論文（学術雑誌）
- 2019年06月, MATERIALS TODAY COMMUNICATIONS, 19, 39 - 50[査読有り]
- 3-Phenyllactic acid (PhLA) is useful as a start-up material in the pharmaceutical and biorefinery industries. To enhance the production of PhLA from glucose using recombinant Escherichia coli, the effects of glucose concentration and oxygen limitation on PhLA production are assessed in a fed-batch system using dissolved oxygen (DO)-stat method. The highest titer of PhLA (7.3 g L-1 ) is observed with a high concentration of glucose and under oxygen-limited conditions (DO = 0 ppm). Under oxygen limitation, cell growth and the formation of acetate and l-phenylalanine (Phe) by-products after 72 h of cultivation are reduced by 30%, 70%, and 81%, respectively, as compared to that under high DO conditions (DO = 5 ppm). Gene expression levels are compared between low and high DO conditions by quantitative polymerase chain reaction (qPCR) analysis. Several genes in the glycolysis (gapA and pykA), pentose phosphate (tktA), and early shikimate pathways for PhLA biosynthesis (aroF, aroG, and aroH) are upregulated under oxygen limitation. The results suggest that oxygen limitation affects metabolism in the shikimate pathway at both metabolic and transcriptional levels and that controlling the DO level is critical for enhanced production of a variety of aromatic compounds through the shikimate pathway.2019年06月, Biotechnology journal, 14(6) (6), e1800478, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Biorefinery has been suggested to provide relevant substitutes to a number of fossil products. Feedstocks and conversion technologies have, however, been the bottleneck to the realization of this concept. Herein, feedstocks and bioconversion technologies under biorefinery have been reviewed. Over the last decade, research has shown possibilities of generating tens of new products but only few industrial implementations. This is partly associated with low production yields and poor cost-competitiveness. This review addresses the technical barriers associated with the conversion of emerging feedstocks into chemicals and bioenergy platforms and summarizes the developed biotechnological approaches including advances in metabolic engineering. This summary further suggests possible future advances that would expand the portfolio of biorefinery and speed up the realization of biofuels and biochemicals.2019年06月, Biotechnology journal, 14(6) (6), e1800494, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- The ability of oleaginous yeast Lipomyces starkeyi to efficiently produce lipids when cultivated on sap extracted from felled oil palm trunk (OPT) as a novel inexpensive renewable carbon source was evaluated. OPT sap was found to contain approximately 98 g/L glucose and 32 g/L fructose. Batch fermentations were performed using three different OPT sap medium conditions: regular sap, enriched sap, and enriched sap at pH 5.0. Under all sap medium conditions, the cell biomass and lipid production achieved were approximately 30 g/L and 60% (w/w), respectively. L. starkeyi tolerated acidified medium (initial pH ≈ 3) and produced considerable amounts of ethanol as well as xylitol as by-products. The fatty acid profile of L. starkeyi was remarkably similar to that of palm oil, one of the most common vegetable oil feedstock used in biodiesel production with oleic acid as the major fatty acid followed by palmitic, stearic and linoleic acids.2019年06月, Journal of bioscience and bioengineering, 127(6) (6), 726 - 731, 英語, 国内誌[査読有り]研究論文（学術雑誌）
- 2019年05月, Nature communications, 10(1) (1), 2040 - 2040, 英語, 国際誌研究論文（学術雑誌）
- Xylitol is a major commodity chemical widely used in both the food and pharmaceutical industries. Although the worldwide demand for xylitol is constantly growing, its industrial production from purified D-xylose involves a costly and polluting catalytic hydrogenation process. Biotechnological production of xylitol from biomass is a promising strategy to establish an environmentally friendly sustainable conversion process. In this study, xylitol was produced from woody Kraft pulp (KP) by using an engineered strain of Saccharomyces cerevisiae (YPH499-XR-BGL-XYL-XYN) expressing cytosolic xylose reductase (XR), along with beta-D-glucosidase (BGL), xylosidase (XYL) and xylanase (XYN) enzymes co-displayed on the cell surface. All these enzymes contributed to the consolidated bioprocessing of KP to xylitol with a yield of 2.3 g L-1 (28% conversion) after 96 hours, along with a significantly reduced amount of commercial enzymes required for pre-treatment (commercial hemicellulase cocktail (CHC), [CHC] = 0.02 g-DW per g). Further improvement of the cell surface display of XYL and XYN was obtained by using a SED1 "SSS" cassette, containing the coding sequences of the SED1 promoter, the SED1 secretion signal, and the SED1 anchoring domain, to generate the improved strain YPH499-XR-BGL-XYLsss-XYNsss. This improved strain showed a significantly enhanced xylitol production capacity reaching a yield of 3.7 g L-1 (44% conversion) after 96 hours. The cellulosic content of KP residues was also significantly increased, from 78% to 87% after 96 hours of fermentation, and nanofibrillation of KP residues was observed by scanning electron microscopy. Pre-treatment and fermentation were successfully performed as a proof of concept to further scale up bio-refinery industrial production of xylitol from lignocellulose.ROYAL SOC CHEMISTRY, 2019年04月, GREEN CHEMISTRY, 21(7) (7), 1795 - 1808, 英語[査読有り]研究論文（学術雑誌）
- The biomass-derived source, low-cost and hydrophobicity/oleophilic advantages of activated carbon (AC) were explored for the immobilization of Aspergillus oryzae whole-cells expressing Fusarium heterosporum lipase. The adsorptive influence of AC favored growth of the cells into its porous interfaces with paralleled exterior dense film formation. Increasing AC weights hindered extracellular lipase activity. Cell aggregation of 0.34 +/- 0.02 mg/BSP was found to be effective in catalyzing an industrially challenging feedstock (68.77% w/w free fatty acids, 20.48% w/w triglycerides) to 98% fatty acid methyl esters (FAME). In a comparative investigation with polyurethane as matrix, higher trans/esterification facilitation was observed with AC. Benefiting from the oleophilicity of AC; denaturation effect from methanol on the lipases was reduced. Surface characterization with FE-SEM, XPS and FT-IR evidenced effective cell-matrix adhesion and a retention of the AC's intrinsic properties. The advantageous tribology of AC ensured recyclability of the matrix for fresh cells immobilization. Comparable FAME (98.08% w/w) was achieved with the recycled matrix in successive batches. The spent-matrix valorization approach, thus, proposes sustainable biorefineries with immobilized lipase catalyzed biodiesel production.AMER CHEMICAL SOC, 2019年03月, ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 7(5) (5), 5010 - 5017, 英語[査読有り]研究論文（学術雑誌）
- Aspergillus oryzae, a filamentous fungus, can secrete large amounts of enzymes extracellularly. We constructed a genetically engineered A. oryzae that simultaneously produced cellobiohydrolase, endoglucanase, and β-glucosidase by integrating multiple copies of the genes encoding these cellulases into fungal chromosomes. The resulting strain possessed 5-16 copies of each cellulase gene within the chromosome and showed approximately 10-fold higher activity versus single integration strains. Copy number polymorphisms were attributed to differences in flanking region sequence for the integrated gene fragments. Furthermore, we found that the P-sodM/T-glaB set demonstrated the strongest transcription levels per gene copy number. We therefore modified promoter/terminator set and cellulase gene combinations based on this polymorphism and transcription level data, with the resulting transformant showing 40-fold higher cellulolytic activity versus the single integration strain. This designed expression method could be useful for the overexpression of multiple enzymes and pathway flux control-mediated metabolic engineering in A. oryzae.2019年03月, Bioresource technology, 276, 146 - 153, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Actinobacteria plays a key role in the cycling of organic matter in soils. They secret biomass-degrading enzymes that allow it to produce the unique metabolites that originate in plant biomass. Although past studies have focused on these unique metabolites, a large-scale screening of Actinobacteria is yet to be reported to focus on their biomass-degrading ability. In the present study, a rapid and simple method is constructed for a large-scale screening, and the novel resources that form the plant biomass-degrading enzyme cocktail are identified from 850 isolates of Actinobacteria. As a result, Nonomuraea fastidiosa secretes a biomass degrading enzyme cocktail with the highest enzyme titer, although cellulase activities are lower than a commercially available enzyme. So the rich accessory enzymes are suggested to contribute to the high enzyme titer for a pretreated bagasse with a synergistic effect. Additionally, an optimized cultivation method of biomass induction caused to produce the improved enzyme cocktail indicated strong enzyme titers and a strong synergistic effect. Therefore, the novel enzyme cocktails are selected via the optimized method for large-scale screening, and then the enzyme cocktail can be improved via the optimized production with biomass-induction.2019年03月, Biotechnology journal, 14(3) (3), e1700744, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- 2019年02月, Biochemical Engineering Journal, 142, 117 - 123, 英語5-Hydroxymethylfurfural production from salt-induced photoautotrophically cultivated Chlorella sorokiniana[査読有り]研究論文（学術雑誌）
- A novel strategy for the low-cost, high-yield co-production of xylose and xylooligosaccharides together with no xylose inhibition was developed using a novel heterologous expression of XYN10Ks_480 endo-1,4-β-xylanase with a ricin-type β-trefoil type of domain and XYN11Ks_480 endo-1,4-β-xylanase with a CBM 2 superfamily from the Kitasatospora sp in an actinomycetes expression system. Xylose is the main building block for hemicellulose xylan. Our findings demonstrated high levels of expression and catalytic activity for XYN10Ks_480 during hydrolysis of the extracted xylan of bagasse, and three types of xylan-based substrates were used to produce xylose and xylooligosaccharides. However, hydrolysis by XYN11Ks_480 produced xylooligosaccharides without xylose formation. This study demonstrated how integrating sodium hypochlorite-extracted xylan and enzymatic hydrolysis could provide an alternative strategy for the generation of XOS from lignocellulosic material.2019年01月, Bioresource technology, 272, 315 - 325, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- © 2019, King Abdulaziz City for Science and Technology. Nanoparticles (NPs) are useful for radiotherapy. Currently, efforts are underway globally for the development of novel titanium dioxide NPs (TiO2-NPs) that exhibit both contrast effects and anti-tumor effects. In this study, the image contrast properties of TiO2-NPs were evaluated using a clinical magnetic resonance imaging (MRI) system and a clinical computed tomography (CT) scanner, as the use of TiO2-NPs as an anti-cancer agent has been reported in several reports. An obvious difference in visualization was observed between the control and TiO2-NP samples on T2-weighted images. These results suggest that TiO2 can potentially be used as a novel theranostic drug with radiosensitizing ability and radiological diagnostic ability, through modification of chemical groups on its surface, and as a component of drug delivery systems.2019年, Applied Nanoscience (Switzerland), 1 - 6, 英語[査読有り]研究論文（学術雑誌）
- © 2018 American Chemical Society. Hydrothermal liquefaction (HTL) is a promising technique for converting biomass feedstocks to fuel and fine chemicals. A metallic Fe additive increases the water-soluble (WS) products of HTL, although the mechanism is unclear. Herein, commercially available carbohydrates (poly- and monosaccharides) and lignin isolated by enzymatic saccharification of palm empty fruit bunch were used as model substrates in the evaluation of the effect of Fe on HTL product composition. For carbohydrates, Fe and oxidized Fe synergistically contributed to the production of light compounds in the WS fraction by accelerating the retro-aldol condensation of sugars and suppressing the recondensation of unstable intermediates. The reactive intermediates could be stabilized by an electron-transfer-type reduction. On the other hand, Fe showed minimal effect on the HTL of enzymatic lignin, which was mainly converted to water-insoluble products. The results for the model substrates provided a picture of the overall pathway of Fe-assisted HTL of biomass.American Chemical Society, 2018年11月, Industrial and Engineering Chemistry Research, 57(44) (44), 14870 - 14877, 英語[査読有り]研究論文（学術雑誌）
- The aim of this study was to construct a cost-effective method for repeated bioethanol production using membrane (ultrafiltration permeation and nanofiltration concentration)-concentrated sweet sorghum juice by using flocculent Saccharomyces cerevisiae F118 strain. With low initial dry cell concentrations at around 0.28-0.35 g L-1, the S. cerevisiae F118 strain provided an ethanol titer of 86.19 ± 1.15 g L-1 (theoretical ethanol yield of 70.77%), which was higher than the non-flocculent S. cerevisiae BY4741 strain at 33.92 ± 0.99 g L-1 after 24 h fermentation. This result was correlated with higher gene expressions of the sucrose-hydrolysing enzyme invertase, sugar phosphorylation, and pyruvate-to-ethanol pathways in the F118 strain compared with the BY4741 strain. Sequential fed-batch fermentation was conducted, and the F118 strain was easily separated from the fermentation broth via the formation of flocs and sediment. After the 5th cycle of fermentation with the F118 strain, the ethanol concentration reached 100.37 g L-1.2018年10月, Bioresource technology, 265, 542 - 547, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- BACKGROUND: Genetic tools including constitutive and inducible promoters have been developed over the last few decades for strain engineering in Streptomyces. Inducible promoters are useful for controlling gene expression, however only a limited number are applicable to Streptomyces. The aim of this study is to develop a controllable protein expression system based on an inducible promoter using sugar inducer, which has not yet been widely applied in Streptomyces. RESULTS: To determine a candidate promoter, inducible protein expression was first examined in Streptomyces avermitilis MA-4680 using various carbon sources. Xylose isomerase (xylA) promoter derived from xylose (xyl) operon was selected due to strong expression of xylose isomerase (XylA) in the presence of D-xylose. Next, a xylose-inducible protein expression system was constructed by investigating heterologous protein expression (chitobiase as a model protein) driven by the xylA promoter in Streptomyces lividans. Chitobiase activity was detected at high levels in S. lividans strain harboring an expression vector with xylA promoter (pXC), under both xylose-induced and non-induced conditions. Thus, S. avermitilis xylR gene, which encodes a putative repressor of xyl operon, was introduced into constructed vectors in order to control protein expression by D-xylose. Among strains constructed in the study, XCPR strain harboring pXCPR vector exhibited strict regulation of protein expression. Chitobiase activity in the XCPR strain was observed to be 24 times higher under xylose-induced conditions than that under non-induced conditions. CONCLUSION: In this study, a strictly regulated protein expression system was developed based on a xylose-induced system. As far as we could ascertain, this is the first report of engineered inducible protein expression in Streptomyces by means of a xylose-induced system. This system might be applicable for controllable expression of toxic products or in the field of synthetic biology using Streptomyces strains.2018年09月, Microbial cell factories, 17(1) (1), 151 - 151, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- A two-stage selection process was applied to eight oleaginous yeast strains from the Lipomyces genera. In the primary selection stage, a nitrogen-limited mineral medium (-NMM) that contained a mixture of glucose and xylose as a carbon source was used to evaluate the lipid-accumulating abilities of the yeast strains. The strains L. doorenjongii, L. orientalis, and L. starkeyi were selected as the potential strains in the primary selection. These three strains exhibited a remarkable ability to simultaneously assimilate glucose and xylose and achieved a cell biomass of more than 30 g/L. The values for lipid content in the selected strains were 57.89 ± 1.92, 56.38 ± 1.93, and 77.14 ± 1.55% for L. doorenjongii, L. orientalis, and L. starkeyi, respectively. In the secondary selection, when the -NMM medium contained an inhibitory chemical compound (ICC), the selected strains showed a different tolerance level against each of the typical inhibitor compounds. However, L. starkeyi accumulated the highest lipid content and yield at 68.24 ± 2.48% and 0.19 ± 0.00 (w/w), respectively. L. starkeyi accumulated high levels of intracellular lipid and tolerated the ICC. The composition of fatty acid methyl esters (FAMEs) was unaltered by the presence of ICC and the major FAMEs consisted of oleic, palmitic, stearic, palmitoleic and linoleic acids.Elsevier B.V., 2018年09月, Biochemical Engineering Journal, 137, 182 - 191, 英語[査読有り]研究論文（学術雑誌）
- (公社)日本生物工学会, 2018年08月, 日本生物工学会大会講演要旨集, 平成30年度, 113 - 113, 日本語油脂酵母Lipomyces starkeyiD35株の繰り返し発酵による脂肪酸組成変化解析
- Hepatitis B virus core (HBc) particles acquire the capacity to disassemble and reassemble in a controlled manner, allowing entrapment and delivery of drugs and macromolecules to cells. HBc particles are made of 180-240 copies of 21 kDa protein monomers, assembled into 30-34 nm diameter icosahedral particles. In this study, we aimed at formulating HBc particles for the delivery of siRNA for gene silencing in vitro and in vivo. We have previously reported recombinant HBc particles expressing ZHER2 affibodies, specifically targeting human epidermal growth receptor 2 (HER2)-expressing cancer cells (ZHER2-ΔHBc). siRNA was encapsulated within the ZHER2-ΔHBc particles following disassembly and reassembly. The ZHER2-ΔHBc-siRNA hybrids were able to secure the encapsulated siRNA from serum and nucleases in vitro. Enhanced siRNA uptake in HER2-expressing cancer cells treated with ZHER2-ΔHBc-siRNA hybrids was observed compared to the nontargeted HBc-siRNA hybrids in a time- and dose-dependent manner. A successful in vitro polo-like kinase 1 (PLK1) gene knockdown was demonstrated in cancer cells treated with ZHER2-ΔHBc-siPLK1 hybrids, to levels comparable to commercial transfecting reagents. Interestingly, ZHER2-ΔHBc particles exhibit intrinsic capability of reducing the solid tumor mass, independent of siPLK1 therapy, in an intraperitoneal tumor model following intraperitoneal injection.2018年07月, ACS applied nano materials, 1(7) (7), 3269 - 3282, 英語, 国際誌研究論文（学術雑誌）
- Ultraviolet-absorbing chemicals are useful in cosmetics and skin care to prevent UV-induced skin damage. We demonstrate here that heterologous production of shinorine, which shows broad absorption maxima in the UV-A and UV-B region. A shinorine producing Corynebacterium glutamicum strain was constructed by expressing four genes from Actinosynnema mirum DSM 43827, which are responsible for the biosynthesis of shinorine from sedoheptulose-7-phosphate in the pentose phosphate pathway. Deletion of transaldolase encoding gene improved shinorine production by 5.2-fold. Among the other genes in pentose phosphate pathway, overexpression of 6-phosphogluconate dehydrogenase encoding gene further increased shinorine production by 60% (19.1 mg/L). The genetic engineering of the pentose phosphate pathway in C. glutamicum improved shinorine production by 8.3-fold in total, and could be applied to produce the other chemicals derived from sedoheptulose-7-phosphate.2018年07月, Bioscience, biotechnology, and biochemistry, 82(7) (7), 1252 - 1259, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Polyacrylic acid (PAA)-modified titanium peroxide nanoparticles (PAA-TiOx NPs) are promising radiosensitizers. PAA-TiOx NPs were synthesized from commercial TiO2 nanoparticles that were modified with PAA and functionalized by H2O2 treatment. To realize practical clinical uses for PAA-TiOx NPs, their tissue distribution and acute toxicity were evaluated using healthy mice and mice bearing tumors derived from xenografted MIAPaCa-2 human pancreatic cancer cells. Healthy mice were injected with PAA-TiOx NPs at 25 mg/kg body weight via the tail vein, and tumor-bearing mice were injected either into the tumor locally or via the tail vein. The concentration of PAA-TiOx NPs in major organs was determined over time using inductively coupled-plasma atomic emission spectrometry. After 1 h, 12% of the PAA-TiOx NP dose had accumulated in the tumor, and 2.8% of the dose remained after 1 week. Such high accumulation could be associated with enhanced permeability and retention effects of the tumor, as PAA-TiOx NPs are composed of inorganic particles and polymers, without tumor-targeting molecules. The liver accumulated the largest proportion of the injected nanoparticles, up to 42% in tumor-bearing mice. Blood biochemical parameters were also investigated after intravenous injection of PAA-TiOx NPs in healthy mice. PAA-TiOx NPs invoked a slight change in various liver-related biochemical parameters, but no liver injury was observed over the practical dose range. In the future, PAA-TiOx NPs should be modified to prevent accumulation in the liver and minimize risk to patients.2018年07月, Journal of Bioscience and Bioengineering, 126(1) (1), 119 - 125, 英語, 国内誌[査読有り]研究論文（学術雑誌）
- Oleaginous microbes can convert substrates such as carbon dioxide, sugars, and organic acids to single-cell oils (SCOs). Among the oleaginous microorganisms, Lipomyces starkeyi is a particularly well-suited host given its impressive native abilities, including the capability to utilize a wide variety of carbon sources. In this work, the potential of L. starkeyi NBRC10381 to produce SCOs in a synthetically nitrogen-limited mineral medium (-NMM) was investigated by differing the inoculum size using glucose and/or xylose as a carbon source. Fermentation using glucose and xylose as mixed carbon sources generated the highest production of biomass at 40.8 g/L, and achieved a lipid content of 84.9% (w/w). When either glucose or xylose was used separately, the totals for achieved lipid content were 79.6% (w/w) and 85.1% (w/w), respectively. However, biomass production was higher for glucose than for xylose (30.3 vs. 28.7 g/L, respectively). This study describes the first simultaneous achievement of higher levels of cell mass and lipid production using glucose and/or xylose as the carbon sources in different inoculum sizes.Elsevier B.V., 2018年06月, Journal of Bioscience and Bioengineering, 125(6) (6), 695 - 702, 英語[査読有り]研究論文（学術雑誌）
- BACKGROUND: L-Arabinose is the second most abundant component of hemicellulose in lignocellulosic biomass, next to D-xylose. However, few microorganisms are capable of utilizing pentoses, and catabolic genes and operons enabling bacterial utilization of pentoses are typically subject to carbon catabolite repression by more-preferred carbon sources, such as D-glucose, leading to a preferential utilization of D-glucose over pentoses. In order to simultaneously utilize both D-glucose and L-arabinose at the same rate, a modified metabolic pathway was rationally designed based on metabolome analysis. RESULTS: Corynebacterium glutamicum ATCC 31831 utilized D-glucose and L-arabinose simultaneously at a low concentration (3.6 g/L each) but preferentially utilized D-glucose over L-arabinose at a high concentration (15 g/L each), although L-arabinose and D-glucose were consumed at comparable rates in the absence of the second carbon source. Metabolome analysis revealed that phosphofructokinase and pyruvate kinase were major bottlenecks for D-glucose and L-arabinose metabolism, respectively. Based on the results of metabolome analysis, a metabolic pathway was engineered by overexpressing pyruvate kinase in combination with deletion of araR, which encodes a repressor of L-arabinose uptake and catabolism. The recombinant strain utilized high concentrations of D-glucose and L-arabinose (15 g/L each) at the same consumption rate. During simultaneous utilization of both carbon sources at high concentrations, intracellular levels of phosphoenolpyruvate declined and acetyl-CoA levels increased significantly as compared with the wild-type strain that preferentially utilized D-glucose. These results suggest that overexpression of pyruvate kinase in the araR deletion strain increased the specific consumption rate of L-arabinose and that citrate synthase activity becomes a new bottleneck in the engineered pathway during the simultaneous utilization of D-glucose and L-arabinose. CONCLUSIONS: Metabolome analysis identified potential bottlenecks in D-glucose and L-arabinose metabolism and was then applied to the following rational metabolic engineering. Manipulation of only two genes enabled simultaneous utilization of D-glucose and L-arabinose at the same rate in metabolically engineered C. glutamicum. This is the first report of rational metabolic design and engineering for simultaneous hexose and pentose utilization without inactivating the phosphotransferase system.BioMed Central Ltd., 2018年05月, Microbial cell factories, 17(1) (1), 76 - 76, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- 日本DDS学会, 2018年05月, 日本DDS学会学術集会プログラム予稿集, 34回, 207 - 207, 日本語新規放射線増感剤としての過酸化チタンナノ粒子の物性解析
- © 2017 We investigated the use of low concentrations of butanol (<40%, all v/v) as an organosolv pretreatment to fractionate lignocellulosic biomass into cellulose, hemicellulose, and lignin. The pretreatment conditions were optimized for sorghum bagasse by focusing on four parameters: butanol concentration, sulfuric acid concentration, pretreatment temperature, and pretreatment time. A butanol concentration of 25% or higher together with 0.5% or higher acid was effective for removing lignin while retaining most of the cellulose in the solid fraction. The highest cellulose (84.9%) and low lignin (15.3%) content were obtained after pretreatment at 200 °C for 60 min. Thus, pretreatment comprising 25% butanol, 0.5% acid, 200 °C, and 60 min process time was considered optimal. Enzymatic saccharification and fermentation by Saccharomyces cerevisiae produced 61.9 g/L ethanol from 200 g/L solid fraction obtained following pretreatment, and 10.2 g/L ethanol was obtained from the liquid fraction by xylose-utilizing S. cerevisiae following membrane nanofiltration to remove butanol.2018年03月, Bioresource Technology, 252, 157 - 164, 英語[査読有り]研究論文（学術雑誌）
- This is the first study to examine the minimum amount of choline acetate (ChOAc), a biocompatible cholinium ionic liquid (IL), for pretreatment of bagasse to achieve the sufficient enzymatic saccharification and co-fermentation from the obtained sugars. Pretreatment of bagasse was conducted using ChOAc at different ionic liquid biomass ratios (IL/biomass ratios of 0–3) to determine the minimum and sufficient IL/biomass ratio for enzymatic saccharification. To apply a small amount of viscous ChOAc to all of the bagasse powder, the ChOAc aqueous solution was first mixed with bagasse powder and the suspension was heated, which pretreated the biomass along with evaporation of water. The minimum IL/biomass ratio was found to be 1.5, which achieved cellulose and hemicellulose saccharification percentages of 95% and 93%, respectively, at low-loading (10 g/L) saccharification. The bagasse pretreated at the IL/biomass ratio of 1.5 was then applied to high-loading saccharification (100 g/L) and subsequent co-fermentation for ethanol production. Glucose and xylose concentrations were 56 g/L and 14 g/L, after 72 h of enzymatic saccharification at high-loading, which corresponded to cellulose and hemicellulose saccharification percentages of 95% and 65%, respectively. In the subsequent co-fermentation from a mixed sugar solution containing 27 g/L glucose and 7 g/L xylose, the ethanol concentration was 15 g/L at 24 h, which was 85% of the theoretical value for the sugar-based ethanol yield.Elsevier B.V., 2018年02月, Chemical Engineering Journal, 334, 657 - 663, 英語[査読有り]研究論文（学術雑誌）
- In the present study, we examined the efficacy of choline acetate (ChOAc, a cholinium ionic liquid))-assisted pretreatment of bagasse powder for subsequent mechanical nanofibrillation to produce lignocellulose nanofibers. Bagasse sample with ChOAc pretreatment and subsequent nanofibrillation (ChOAc/NF-bagasse) was prepared and compared to untreated control bagasse sample (control bagasse), bagasse sample with nanofibrillation only (NF-bagasse) and with ChOAc pretreatment only (ChOAc-bagasse). The specific surface area was 0.83 m2/g, 3.1 m(2)/g, 6.3 m(2)/g, and 32 m(2)/g for the control bagasse, ChOAc-bagasse, NF-bagasse, and the ChOAc/NF-bagasse, respectively. Esterified bagasse/polypropylene composites were prepared using the bagasse samples. ChOAc/NF-bagasse exhibited the best dispersion in the composites. The tensile toughness of the composites was 0.52 J/cm(3), 0.73 J/cm(3), 0.92 J/cm(3), and 1.29 J/cm(3) for the composites prepared using control bagasse, ChOAcbagasse, NF-bagasse, and ChOAc/NF-bagasse, respectively. Therefore, ChOAc pretreatment and subsequent nanofibrillation of bagasse powder resulted in enhanced tensile toughness of esterified bagasse/polypropylene composites.ELSEVIER SCI LTD, 2018年02月, CARBOHYDRATE POLYMERS, 182, 8 - 14, 英語[査読有り]研究論文（学術雑誌）
- 一般社団法人 日本エネルギー学会, 2018年, バイオマス科学会議発表論文集, 13(0) (0), 135 - 136, 日本語
Caffeic acid (3,4-dihydroxycinnamic acid) serves as a building block for thermoplastics and was recently produced from glucose by microbial fermentation. To produce caffeic acid from inedible cellulose, separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) reactions were compared using kraft pulp as lignocellulosic feedstock. Here, a tyrosine-overproducing Escherichia coli strain was metabolically engineered to produce caffeic acid from glucose by expressing the genes encoding a tyrosine ammonia lyase (fevV) from Streptomyces sp. WK-5344 and 4-hydroxyphenyllactate 3-hydroxylase (hpaBC) from Pseudomonas aeruginosa. Using the resulting recombinant strain, the maximum yield of caffeic acid in SSF (233 mg/L) far exceeded that by SHF (37.9 mg/L). In the SSF with low cellulase loads (≤2.5 filter paper unit/g glucan), caffeic acid production was markedly increased, while almost no glucose accumulation was detected, indicating that the E. coli cells experienced glucose limitation in this culture condition. Caffeic acid yield was also negatively correlated with the glucose concentration in the fermentation medium. These observations suggest that glucose limitation in SSF is crucial for improving caffeic acid yield, owing to reduced by-product formation and fermentation inhibitor accumulation.
[査読有り] - This study compared the oxidative depolymerization potential between ionic liquid (IL)-pretreated/enzyme lignin, diluted acid (DA)-pretreated/enzyme lignin, and soda lignin of eucalyptus, by means of alkaline nitrobenzene or cupric oxide (CuO) oxidation which depolymerize lignin into phenolic aldehydes such as vanillin and syringaldehyde. Here, the IL-pretreated/enzyme lignin was prepared by 1-ethyl-3-methylimidazolium acetate pretreatment and subsequent enzymatic saccharification. Upon the oxidative depolymerization of lignin sample by alkaline nitrobenzene oxidation, total yield of vanillin and syringaldehyde was 36.6% for untreated control eucalyptus. In contrast, the yield was enhanced upto 48.0% for IL-pretreated/enzyme lignin. As for the soda lignin prepared by alkali cooking, the yield was 29.0%. As for the DA-pretreated/enzyme lignin prepared by DA pretreatment and subsequent enzymatic saccharification, the yield was 39.5%. Therefore, it was found that the IL-pretreated/enzyme lignin showed the highest yield of phenolic aldehydes (vanillin and syringaldehyde) via alkaline oxidation, namely, highest oxidative depolymerization potential among the lignin tested.Elsevier B.V., 2018年01月, Industrial Crops and Products, 111, 457 - 461, 英語研究論文（学術雑誌）
- The addition of enzymes that are capable of degrading hemicellulose has a potential to reduce the need for commercial enzymes during biomass hydrolysis in the production of fermentable sugars. In this study, a high xylanase producing actinomycete strain (Kitasatospora sp. ID06-480) and the first ethyl ferulate producing actinomycete strain (Nonomuraea sp. ID06-094) were selected from 797 rare actinomycetes, respectively, which were isolated in Indonesia. The addition (30%, v/v) of a crude enzyme supernatant from the selected strains in sugarcane bagasse hydrolysis with low-level loading (1 FPU/g-biomass) of Cellic® CTec2 enhanced both the released amount of glucose and reducing sugars. When the reaction with Ctec2 was combined with crude enzymes containing either xylanase or feruloyl esterase, high conversion yield of glucose from cellulose at 60.5% could be achieved after 72 h-saccharification.Japan Society for Bioscience Biotechnology and Agrochemistry, 2018年, Bioscience, Biotechnology and Biochemistry, 82(5) (5), 904 - 915, 英語[査読有り]研究論文（学術雑誌）
- This study was focused on the intensification of alpha amylase activity by fungi. Submerged culture of Aspergillus oryzae as a non-Newtonian fluid with a complex rheology and morphology was used. The aim of this study was to enhance the hydrodynamics of the biological fluid for an improvement in cell-gas mass transfer. Therefore, we investigated the advantages of using a Swingstir® agitator that features an angular motion. Results showed that mixing time, mixing energy, and power-law model parameters using the Swingstir® all were lower than when a double Rushton turbine (DRT) was used at the same level of power consumption. The Swingstir® can produces a homogeneous and periodic-flow distribution. Moreover, KLa could be controlled throughout the fermentation process. Also, the morphology of pellets agitated by Swingstir® had a positive effect on mass transfer intensification, because the stress is applied by flexible shaft movement instead by the blade tip of impeller. Consequently, the alpha amylase activity and cell growth of a culture agitated by Swingstir® were higher than that developed using a DRT.Society of Chemical Engineers, Japan, 2018年, Journal of Chemical Engineering of Japan, 51(2) (2), 143 - 151, 英語[査読有り]研究論文（学術雑誌）
- © 2018, Genetics Society of Japan. All rights reserved. Wheat straw is one of the major attractive resources for low-cost raw materials for renewable energy, biofuels and biochemicals. However, like other sources of lignocellulosic biomass, straw is a heterogeneous material due to its mixed origin from different tissue and cell types. Here, to examine the genotypic effects on biorefinery usage of wheat straw, straw obtained from different wheat cultivars and experimental lines was pretreated with dilute acid. Significant differences between cultivars were observed in the concentrations of glucose and toxic byproducts of the liquid hydrolysates. A higher content of xylose than glucose was found in liquid hydrolysates from wheat straw, and the xylose content appeared to be affected by both environmental and genetic factors. Analysis using chromosome substitution lines of the common wheat cultivar Chinese Spring showed that chromosomes 2A and 3A from other wheat cultivars, Hope and Timstein, significantly increased the xylose content. However, no significant relationship was observed between the liquid hydrolysate xylose content and the glucose content obtained from enzymatic saccharification of the acid-insoluble residue. These results highlight the potential of wheat breeding to improve biomass-related traits in wheat straw.日本遺伝学会, 2018年, Genes and Genetic Systems, 93(1) (1), 1 - 7, 英語[査読有り]研究論文（学術雑誌）
- Background: The cyanobacterium Arthrospira platensis shows promise as a carbohydrate feedstock for biofuel production. The glycogen accumulated in A. platensis can be extracted by lysozyme-degrading the peptidoglycan layer of the bacterial cell walls. The extracted glycogen can be converted to ethanol through hydrolysis by amylolytic enzymes and fermentation by the yeast Saccharomyces cerevisiae. Thus, in the presence of lysozyme, a recombinant yeast expressing α-amylase and glucoamylase can convert A. platensis directly to ethanol, which would simplify the procedure for ethanol production. However, the ethanol titer and productivity in this process are lower than in ethanol production from cyanobacteria and green algae in previous reports. Results: To increase the ethanol titer, a high concentration of A. platensis biomass was employed as the carbon source for the ethanol production using a recombinant amylase-expressing yeast. The addition of lysozyme to the fermentation medium increased the ethanol titer, but not the ethanol productivity. The addition of CaCl2 increased both the ethanol titer and productivity by causing the delamination of polysaccharide layer on the cell surface of A. platensis. In the presence of lysozyme and CaCl2, ethanol titer, yield, and productivity improved to 48 g L-1, 93% of theoretical yield, and 1.0 g L-1 h-1 from A. platensis, corresponding to 90 g L-1 of glycogen. Conclusions: We developed an ethanol conversion process using a recombinant amylase-expressing yeast from A. platensis with a high titer, yield, and productivity by adding both lysozyme and CaCl2. The direct and highly productive conversion process from A. platensis via yeast fermentation could be applied to multiple industrial bulk chemicals.BioMed Central Ltd., 2018年, Biotechnology for biofuels, 11(1) (1), 50 - 50, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Measuring the concentrations of metabolites and estimating the reaction rates of each reaction step consisting of metabolic pathways are significant for an improvement in microorganisms used in maximizing the production of materials. Although the reaction pathway must be identified for such an improvement, doing so is not easy. Numerous reaction steps have been reported; however, the actual reaction steps activated vary or change according to the conditions. Furthermore, to build mathematical models for a dynamical analysis, the reaction mechanisms and parameter values must be known; however, to date, sufficient information has yet to be published for many cases. In addition, experimental observations are expensive. A new mathematical approach that is applicable to small sample data, and that requires no detailed reaction information, is strongly needed. S-system is one such model that can use smaller samples than other ordinary differential equation models. We propose a simplified S-system to apply minimal quantities of samples for a dynamic analysis of the metabolic pathways. We applied the model to the phenyl lactate production pathway of Escherichia coli. The model obtained suggests that actually activated reaction steps and feedback are inhibitions within the pathway.2018年, Bioinformatics and biology insights, 12, 1177932218775076 - 1177932218775076, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- © 2018, Genetics Society of Japan. All rights reserved. Wheat straw is one of the major attractive resources for low-cost raw materials for renewable energy, biofuels and biochemicals. However, like other sources of lignocellulosic biomass, straw is a heterogeneous material due to its mixed origin from different tissue and cell types. Here, to examine the genotypic effects on biorefinery usage of wheat straw, straw obtained from different wheat cultivars and experimental lines was pretreated with dilute acid. Significant differences between cultivars were observed in the concentrations of glucose and toxic byproducts of the liquid hydrolysates. A higher content of xylose than glucose was found in liquid hydrolysates from wheat straw, and the xylose content appeared to be affected by both environmental and genetic factors. Analysis using chromosome substitution lines of the common wheat cultivar Chinese Spring showed that chromosomes 2A and 3A from other wheat cultivars, Hope and Timstein, significantly increased the xylose content. However, no significant relationship was observed between the liquid hydrolysate xylose content and the glucose content obtained from enzymatic saccharification of the acid-insoluble residue. These results highlight the potential of wheat breeding to improve biomass-related traits in wheat straw.2018年, Genes and Genetic Systems, 93(1) (1), 1 - 7, 英語[査読有り]研究論文（学術雑誌）
- Ferulic acid (4-hydroxy-3-methoxycinnamic acid, FA) is a lignin-derived phenolic compound abundant in plant biomass. The utilization of FA and its conversion to valuable compounds is desired. Protocatechuic acid (3,4-dihydroxybenzoic acid, PCA) is a precursor of polymers and plastics and a constituent of food. A microbial conversion system to produce PCA from FA was developed in this study using a PCA-producing strain of Corynebacterium glutamicum F (ATCC 21420). C. glutamicum strain F grown at 30 °C for 48 h utilized 2 mM each of FA and vanillic acid (4-hydroxy-3-methoxybenzoic acid, VA) to produce PCA, which was secreted into the medium. FA may be catabolized by C. glutamicum through proposed (I) non-β-oxidative, CoA-dependent or (II) β-oxidative, CoA-dependent phenylpropanoid pathways. The conversion of VA to PCA is the last step in each pathway. Therefore, the vanillate O-demethylase gene (vanAB) from Corynebacterium efficiens NBRC 100395 was expressed in C. glutamicum F (designated strain FVan) cultured at 30 °C in AF medium containing FA. Strain C. glutamicum FVan converted 4.57 ± 0.07 mM of FA into 2.87 ± 0.01 mM PCA after 48 h with yields of 62.8% (mol/mol), and 6.91 mM (1064 mg/L) of PCA was produced from 16.0 mM of FA after 12 h of fed-batch biotransformation. Genomic analysis of C. glutamicum ATCC 21420 revealed that the PCA-utilization genes (pca cluster) were conserved in strain ATCC 21420 and that mutations were present in the PCA importer gene pcaK.2017年12月, AMB Express, 7(1) (1), 130 - 130, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Bioproduction using microbes from biomass feedstocks is of interest in regards to environmental problems and cost reduction. Streptomyces as an industrial microorganism plays an important role in the production of useful secondary metabolites for various applications. This strain also secretes a wide range of extracellular enzymes which degrade various biopolymers in nature, and it consumes these degrading substrates as nutrients. Hence, Streptomyces can be employed as a cell factory for the conversion of biomass-derived substrates into various products. This review focuses on the following two points: (1) Streptomyces as a producer of enzymes for degrading biomass-derived polysaccharides and polymers; and, (2) wild-type and engineered strains of Streptomyces as a host for chemical production from biomass-derived substrates. (C) 2017 Elsevier Ltd. All rights reserved.ELSEVIER SCI LTD, 2017年12月, BIORESOURCE TECHNOLOGY, 245, 1655 - 1663, 英語[査読有り]研究論文（学術雑誌）
- The worldwide market for plastics is rapidly growing, and plastics polymers are typically produced from petroleum-based chemicals. The overdependence on petroleum-based chemicals for polymer production raises economic and environmental sustainability concerns. Recent progress in metabolic engineering has expanded fermentation products from existing aliphatic acids or alcohols to include aromatic compounds. This diversity provides an opportunity to expand the development and industrial uses of high-performance bio-based polymers. However, most of the biomonomers are produced from edible sugars or starches that compete directly with food and feed uses. The present review focuses on recent progress in the microbial conversion of biomass into bio-based polymers, in which fermentative products from renewable feedstocks serve as biomonomers for the synthesis of bio-based polymers. In particular, the production of biomonomers from inedible lignocellulosic feedstocks by metabolically engineered microorganisms and the synthesis of bio-based engineered plastics from the biological resources are discussed. (C) 2017 Elsevier Ltd. All rights reserved.ELSEVIER SCI LTD, 2017年12月, BIORESOURCE TECHNOLOGY, 245, 1664 - 1673, 英語[査読有り]研究論文（学術雑誌）
- Filamentous fungi exhibit versatile abilities, including organic acid fermentation, protein production, and secondary metabolism, amongst others, and thus have applications in the medical and food industries. Previous genomic analyses of several filamentous fungi revealed their further potential as host microorganisms for bioproduction. Recent advancements in molecular genetics, marker recycling, and genome editing could be used to alter transformation and metabolism, based on optimized design carbolated with computer science. In this review, we detail the current applications of filamentous fungi and describe modern molecular genetic tools that could be used to expand the role of these microorganisms in bioproduction. The present review shed light on the possibility of filamentous fungi as host microorganisms in the field of bioproduction in the future. (C) 2017 Elsevier Ltd. All rights reserved.ELSEVIER SCI LTD, 2017年12月, BIORESOURCE TECHNOLOGY, 245, 1314 - 1326, 英語[査読有り]研究論文（学術雑誌）
- This work aimed to study the use of consolidated bioprocess (CBP) yeast expressing five cellulase genes (BGL, XYNII, EGII, CBHI and CBHII) for ethanol production from ionic liquid-pretreated bagasse and Laubholz unbleached Kraft pulp (LUKP). A proposed screening method shows that the optimal cellulase ratio varies for each biomass substrate, and thus it is essential to breed CBP yeast having optimal cellulase-displaying ratio for the target biomass. CBP yeast specialized towards bagasse produced 0.93 g/l ethanol whiles that for LUKP produced 0.71 g/l ethanol, which is approximately 4 and 2-fold, respectively, higher than that of the wild type. The cell-surface displayed enzymes synergistically contributed to the degradation of the biomass. The developed CBP yeast is a potential cheap source for consolidated bioprocessing of ethanol and the proposed screening method can be used for matching CBP yeast to a target biomass. (C) 2017 Published by Elsevier Ltd.ELSEVIER SCI LTD, 2017年12月, BIORESOURCE TECHNOLOGY, 245, 1413 - 1420, 英語[査読有り]研究論文（学術雑誌）
- This study provides insight observation based on the gene expression and the metabolomic analysis of the natural robust yeast Saccharomyces cerevisiae NBRC849 during the fermentation in the medium containing inhibitory chemical complexes (ICC) at different concentrations. The tolerance mechanisms involved in the strain might have existed through the upregulation of genes involved in NAD(H)/NADP (H) cofactors generations (ALD6, ZWF1, GND1), membrane robustness for efflux pump (YOR1, PDR5, TPO3) and cation/polyamine transport (TPO3). The alteration of metabolic flux to the shikimic pathway was also found in this strain, resulted in the enhanced formation of aromatic amino acid required for cell survival. Enhanced expression of these genes as well as the increase of metabolic flux to shikimic pathway were suggested to result in the robustness of non-flocculating S. cerevisiae haploid strain. (C) 2017 Elsevier Ltd. All rights reserved.ELSEVIER SCI LTD, 2017年12月, BIORESOURCE TECHNOLOGY, 245, 1436 - 1446, 英語[査読有り]研究論文（学術雑誌）
- For preparation of wood-plastic composites, pretreatment of lignocellulosic material is important to increase compatibility between matrix polymers and lignocellulosic fillers. In this study, choline acetate (ChOAc, a cholinium ionic liquid) assisted pretreatment of bagasse powder could improve properties of chemically modified bagasse/polypropylene composites. The composites had fewer voids derived from vascular bundles of the bagasse tissue when using ChOAc-pretreated bagasse powder compared with the case when using untreated bagasse powder. The tensile strength of the composites was 20 MPa, 35 MPa, and 40 MPa in the cases of polypropylene only, untreated bagasse powder, and ChOAc-pretreated bagasse powder, respectively. The elastic modulus was 0.7 GPa, 2.0 GPa, and 2.6 GPa in the case of polypropylene only, the case using untreated bagasse powder, and the case using ChOAc-pretreated bagasse powder, respectively. Thus, ChOAc pretreatment of bagasse powder could enhance the blending and the tensile property of propionylated bagasse/polypropylene composites compared with those of the composites made using untreated bagasse powder.ELSEVIER SCIENCE BV, 2017年12月, INDUSTRIAL CROPS AND PRODUCTS, 109, 158 - 162, 英語[査読有り]研究論文（学術雑誌）
- This work aims to produce glutathione directly from mannan-based bioresources using engineered Saccharomyces cerevisiae. Mannan proved to be a valuable carbon source for glutathione production by this organism. Mannan-hydrolyzing S. cerevisiae was developed by heterologous expression of mannanase/ mannosidase on its cell surface. This strain efficiently produced glutathione from mannose polysaccharide, beta-1,4-mannan. Furthermore, it produced glutathione from locust bean gum (LBG), a highly dense and inexpensive mannan-based bioresource, as sole carbon source. Glutathione productivity from LBG was enhanced by engineering the glutathione metabolism of mannan-hydrolyzing S. cerevisiae. Expression of extracellular mannanase/mannosidase protein combined with intracellular metabolic engineering is potentially applicable to the efficient, environmentally friendly bioproduction of targeted products from mannan-based bioresources. (C) 2017 Elsevier Ltd. All rights reserved.ELSEVIER SCI LTD, 2017年12月, BIORESOURCE TECHNOLOGY, 245, 1400 - 1406, 英語[査読有り]研究論文（学術雑誌）
- Lipid from Chlamydomonas sp. JSC4 was used as a feedstock for biodiesel production. The lipid was found to contain high amounts of phospholipids and free fatty acid in addition to the triglycerides. Two enzymatic methods for the efficient conversion of the heterogenous lipid to fatty acid methyl esters (FAME) were carried out. The method using either a lipase cocktail containing Candida cylindracea lipase and Thermomyces lanuginosus lipase combination (m I) or immobilized Fusarium heterosporum lipase-expressing Aspergillus oryzae whole-cells (m II) were both successful. However, the method using lipase cocktail showed 30.8% relative stability after the fourth batch, whereas the whole-cell biocatalyst showed 98.1%. Although the whole-cell biocatalyst tolerated a wide range of water content, an exploration of the effect of water-methanol interaction on the biocatalytic process showed that 24% water and 7: 1 methanol to oil ratio is more favorable for FAME production. A higher initial methanol consumption rate facilitated a more stable system with the whole-cell biocatalyst, producing over 97% FAME in 32 h. The efficient conversion of a highly heterogenous substrate in the presence of high amounts of water could be an effective technique for the enzymatic conversion of microalgal lipids.ELSEVIER SCIENCE BV, 2017年12月, ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 28, 16 - 23, 英語[査読有り]研究論文（学術雑誌）
- Surgical site infections continue to be a common complication affecting surgical prognosis. Reactive oxygen species (ROS) are generated by ultrasound-irradiated titanium dioxide (TiO2) (UIT). Although excessive ROS production can cause cell damage, ROS at physiological levels mediate beneficial cellular responses, including angiogenesis. This study investigated whether UIT can promote healing of Escherichia coli-infected wounds. We used TiO2 and ultrasound irradiation using an ultrasonography machine at a frequency of 1.0 MHz and intensity of 0.4 W cm−2. These levels are not bactericidal in vitro therefore, we could study the effect of UIT on E. coli survival without interference of ultrasound effects. The number of cluster of differentiation 31-positive blood vessels, which are indicative of angiogenesis, was decreased by bacterial infection, and increased at the wound edges in the UIT-treated infected wounds, suggesting upregulation of neovascularization by UIT. Although UIT treatment did not decrease E. coli survival in vivo, it promoted healing of the infected wounds as evidenced by a significant decrease in the wound area in the UIT-treated mice. Our findings demonstrate that UIT promotes wound healing in surgical site infections and suggest beneficial use of the UIT-based approach as a novel therapeutic method to treat infected surgical wounds. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2344–2351, 2017.John Wiley and Sons Inc., 2017年11月, Journal of Biomedical Materials Research - Part B Applied Biomaterials, 105(8) (8), 2344 - 2351, 英語[査読有り]研究論文（学術雑誌）
- A sensing system was constructed to monitor the target peptide via two aptamer-based sensors pinching. First, aptamers against somatostatin (SST) were selected via the systematic evolution of ligands by exponential enrichment, and four aptamers were selected from a single-stranded DNA library. Their specificities to SST were evaluated via surface plasmon resonance and atomic force microscopy (AFM). Next, two aptamers with higher specificities to SST were used as aptamer-based sensors; one aptamer was modified with a chip, and the other was modified with a probe. Based on AFM system, the probe was surveyed on the chip in SST solutions, simultaneously measuring an interactive force. The label-free SST could be detected, and then the change in its concentration could be monitored at levels that ranged from 2 to 2000 nM. The interactive force of a single pair was approximately 45 pN, and the molecule number was associated with the interactive force. Therefore, we could firstly select the aptamers against somatostatin, and the sandwich-like monitoring system can be used to promote peptide sensor or monitoring system using an aptamer-based sensor. (C) 2017 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE SA, 2017年11月, SENSORS AND ACTUATORS B-CHEMICAL, 252, 813 - 821, 英語[査読有り]研究論文（学術雑誌）
- A split aptamers-based aptasensor is applied to peptide sensing using atomic force microscopy (AFM). Somatostatin (SST) is targeted as a model peptide. The aptamer against SST is split, and each part is chemically modified with a probe or a chip. Hybridized aptamers can detect label-free SSTs and monitor changes in their concentration from 2 nM to 2000 nM as an index of interactive force. The change in an interactive force could be firstly evaluated via Hill model. Additionally, the analysis based on the fitting enables clarification of the differences in hybridization and in interaction, as well as an estimation of the number of SSTs. The proposed sensing system can promote peptide sensing based on the use of an aptasensor. (C) 2017 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE SA, 2017年11月, SENSORS AND ACTUATORS B-CHEMICAL, 252, 600 - 605, 英語[査読有り]研究論文（学術雑誌）
- The juice from sweet sorghum cultivar SIL-05 (harvested at physiological maturity) was extracted, and the component sucrose and reducing sugars (such as glucose and fructose) were subjected to a membrane separation process to purify the sucrose for subsequent sugar refining and to obtain a feedstock for repeated bioethanol production. Nanofiltration (NF) of an ultrafiltration (UF) permeate using an NTR-7450 membrane (Nitto Denko Corporation, Osaka, Japan) concentrated the juice and produced a sucrose-rich fraction (143.2 g L-1 sucrose, 8.5 g L-1 glucose, and 4.5 g L-1 fructose). In addition, the above NF permeate was concentrated using an ESNA3 NF membrane to provide concentrated permeated sugars (227.9 g L-1) and capture various amino acids in the juice, enabling subsequent ethanol fermentation without the addition of an exogenous nitrogen source. Sequential batch fermentation using the ESNA3 membrane concentrate provided an ethanol titer and theoretical ethanol yield of 102.5-109.5 g L-1 and 84.4-89.6%, respectively, throughout the five-cycle batch fermentation by Saccharomyces cerevisiae BY4741. Our results demonstrate that a membrane process using UF and two types of NF membranes has the potential to allow sucrose purification and repeated bioethanol production.2017年08月, Applied microbiology and biotechnology, 101(15) (15), 6007 - 6014, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Caffeic acid (3,4-dihydroxycinnamic acid) serves as a building block for thermoplastics and a precursor for biologically active compounds and was recently produced from glucose by microbial fermentation. To produce caffeic acid from inedible cellulose, separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) reactions were compared using kraft pulp as lignocellulosic feedstock. Here, a tyrosine-overproducing Escherichia coli strain was metabolically engineered to produce caffeic acid from glucose by introducing the genes encoding a 4-hydroxyphenyllactate 3-hydroxylase (hpaBC) from Pseudomonas aeruginosa and tyrosine ammonia lyase (fevV) from Streptomyces sp. WK-5344. Using the resulting recombinant strain, the maximum yield of caffeic acid in SSF (233 mg/L) far exceeded that by SHF (37.9 mg/L). In the SSF with low cellulase loads (ae<currency>2.5 filter paper unit/g glucan), caffeic acid production was markedly increased, while almost no glucose accumulation was detected, indicating that the E. coli cells experienced glucose limitation in this culture condition. Caffeic acid yield was also negatively correlated with the glucose concentration in the fermentation medium. In SHF, the formation of by-product acetate and the accumulation of potential fermentation inhibitors increased significantly with kraft pulp hydrolysate than filter paper hydrolysate. The combination of these inhibitors had synergistic effects on caffeic acid fermentation at low concentrations. With lower loads of cellulase in SSF, less potential fermentation inhibitors (furfural, 5-hydroxymethyfurfural, and 4-hydroxylbenzoic acid) accumulated in the medium. These observations suggest that glucose limitation in SSF is crucial for improving caffeic acid yield, owing to reduced by-product formation and fermentation inhibitor accumulation.SPRINGER, 2017年07月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 101(13) (13), 5279 - 5290, 英語[査読有り]研究論文（学術雑誌）
- Among the many types of lignocellulosic biomass pretreatment methods, the use of ionic liquids (ILs) is regarded as one of the most promising strategies. In this study, the effects of four kinds of ILs for pretreatment of lignocellulosic biomass such as bagasse, eucalyptus, and cedar were evaluated. In direct ethanol fermentation from biomass incorporated with ILs by cellulase-displaying yeast, 1-butyl-3-methylimidazolium acetate ([Bmim][OAc]) was the most effective IL. The ethanol production and yield from [Bmim][OAc]-pretreated bagasse reached 0.81 g/L and 73.4% of the theoretical yield after fermentation for 96 h. The results prove the initial concept, in which the direct fermentation from lignocellulosic biomass effectively promoted by the pretreatment with IL.HUMANA PRESS INC, 2017年05月, APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 182(1) (1), 229 - 237, 英語[査読有り]研究論文（学術雑誌）
- Mannan endo-1,4-beta-mannosidase(commonly known as beta-mannanase) catalyzes a random cleavage of the beta-D-1,4mannopyranosyl linkage in mannan polymers. The enzyme has been utilized in biofuel production from lignocellulose biomass, as well as in production of mannooligosaccharides (MOS) for applications in feed and food industries. We aimed to obtain a beta-mannanase, for such mannan polymer utilization, from actinomycetes strains isolated in Indonesia. Strains exhibiting high mannanase activity were screened, and one strain belonging to the genus Kitasatospora was selected. We obtained a beta-mannanase from this strain, and an amino acid sequence of this Kitasatospora beta-mannanase showed a 58-71% similarity with the amino acid sequences of Streptomyces beta-mannanases. The Kitasatospora beta-mannanase showed a significant level of activity (944 U/mg) against locust bean gum (0.5% w/v) and a potential for oligosaccharide production from various mannan polymers. The beta-mannanase might be beneficial particularly in the enzymatic production of MOS for applications of mannan utilization.BIOMED CENTRAL LTD, 2017年05月, AMB Express, 7, 英語[査読有り]研究論文（学術雑誌）
- An E. coli expression system offers a mean for rapid, high yield and economical production of Hepatitis B Virus core (HBc) particles. However, high-level production of HBc particles in bacteria is demanding and optimisation of HBc particle yield from E. coli is required to improve laboratory-scale productivity for further drug delivery applications. Production steps involve bacterial culture, protein isolation, denaturation, purification and finally protein assembly. In this study, we describe a modified E. coli based method for purifying HBc particles and compare the results with those obtained using a conventional purification method. HBc particle morphology was confirmed by Atomic Force Microscopy (AFM). Protein specificity and secondary structure were confirmed by Western Blot and Circular Dichroism (CD), respectively. The modified method produced similar to 3-fold higher yield and greater purity of wild type HBc particles than the conventional method. Our results demonstrated that the modified method produce a better yield and purity of HBc particles in an E. coli-expression system, which are fully characterised and suitable to be used for drug delivery applications.NATURE PUBLISHING GROUP, 2017年03月, SCIENTIFIC REPORTS, 7, 43160, 英語[査読有り]研究論文（学術雑誌）
- The presence of high levels of free fatty acids (FFA) in oil is a barrier to one-step biodiesel production. Undesirable soaps are formed during conventional chemical methods, and enzyme deactivation occurs when enzymatic methods are used. This work investigates an efficient technique to simultaneously convert a mixture of free fatty acids and triglycerides (TAG). A partial soybean hydrolysate containing 73.04% free fatty acids and 24.81% triglycerides was used as a substrate for the enzymatic production of fatty acid methyl ester (FAME). Whole-cell Candida antarctica lipase B-expressing Aspergillus oryzae, and Novozym 435 produced only 75.2 and 73.5% FAME, respectively. Fusarium heterosporum lipase-expressing A. oryzae produced more than 93% FAME in 72 h using three molar equivalents of methanol. FFA and TAG were converted simultaneously in the presence of increasing water content that resulted from esterification. Therefore, F. heterosporum lipase with a noted high level of tolerance of water could be useful in the industrial production of biodiesel from feedstock that has high proportion of free fatty acids.WILEY-V C H VERLAG GMBH, 2017年03月, BIOTECHNOLOGY JOURNAL, 12(3) (3), 1600400, 英語[査読有り]研究論文（学術雑誌）
- The surface of yeast cells has been an attractive interface for the effective use of cellulose. Surface enzymes, however, are difficult to visualize and evaluate. In this study, two kinds of unique anchoring regions were used to display the cellulase, endoglucanase (EG), on a yeast cell surface. Differences in the display level and the localization of EG were observed by atomic force microscopy. By surveying the yeast cell surface with a chemically modified cantilever, the interactive force between the cellulose and EG was measured. Force curve mapping revealed differences in the display levels and the localization of EG according to anchoring regions. The proposed methodology enables visualization of displayed enzymes such as EG on the yeast cell surface. (C) 2016 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, 2017年03月, COLLOIDS AND SURFACES B-BIOINTERFACES, 151, 134 - 142, 英語[査読有り]研究論文（学術雑誌）
- Hepatitis B Virus core (HBc) particles have been studied for their potential as drug delivery vehicles for cancer therapy. HBc particles are hollow nano-particles of 30-34 nm diameter and 7 nm thick envelopes, consisting of 180-240 units of 21 kDa core monomers. They have the capacity to assemble/dis-assemble in a controlled manner allowing encapsulation of various drugs and other biomolecules. Moreover, other functional motifs, i.e. receptors, receptor binding sequences, peptides and proteins can be expressed. This study focuses on the development of genetically modified HBc particles to specifically recognise and target human epidermal growth factor receptor-2 (HER2)-expressing cancer cells, in vitro and in vivo, for future cancer therapy. The non-specific binding capacity of wild type HBc particles was reduced by genetic deletion of the sequence encoding arginine-rich domains. A specific HER2-targeting was achieved by expressing the ZHER2 affibodies on the HBc particles surface. In vitro studies showed specific uptake of ZHER2-AHBc particles in HER2 expressing cancer cells. In vivo studies confirmed positive uptake of ZHER2-ABBc particles in HER2-expressing tumours, compared to non-targeted AHBc particles in intraperitoneal tumour-bearing mice models. The present results highlight the potential of these nanocarriers in targeting HER2-positive metastatic abdominal cancer following intra-peritoneal administration. (C) 2016 The Authors. Published by Elsevier Ltd.ELSEVIER SCI LTD, 2017年03月, BIOMATERIALS, 120, 126 - 138, 英語[査読有り]研究論文（学術雑誌）
- DNA-duplex interactions in thymines and adenins are used as a linker for the novel methodology of Atomic Force Microscope-Systematic Evolution of Ligands by EXpotential enrichment (AFM-SELEX). This study used the hydrogen bonds in 10 mer of both thymines (T10) and adenines (A10). Initially, the interactive force in T10-A10 was measured by AFM, which returned an average interactive force of approximately 350 pN. Based on this result, DNA aptamers against human serum albumin could be selected in the 4th round, and 15 different clones could be sequenced. The lowest dissociation constant of the selected aptamer was identified via surface plasmon resonance, and it proved to be identical to that of the commercial aptamer. Therefore, specific hydrogen bonds in DNA can be useful linkers for AFMSELEX. (C) 2016 Elsevier Ltd. All rights reserved.PERGAMON-ELSEVIER SCIENCE LTD, 2017年02月, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 27(4) (4), 954 - 957, 英語[査読有り]研究論文（学術雑誌）
- A Fullzone (FZ) impeller was used in the first study of the characteristics involved in the fermentation of Aspergillus oryzae. Both the experimental and simulation results of this study revealed novel findings into the positive relationship between the global-axial mixing patterns of a FZ impeller and fermentation efficiency. The mixing results when using the FZ impeller compared with a double Rushton turbine (DRT) impeller indicated that the culture mixed by the FZ resulted in a more homogeneous medium with higher values for oxygen mass transfer, cell growth rate, and alpha amylase activity. The simulation of fluid flow was done in a laminar regime using a two-fluid model. According to the simulation results, the maximum shear stress when using the DRT was higher than that with the FZ at the same power input (P-in). A high degree of local shear stress and the shear rate near the turbine blade of the DRT resulted in cell damage and a reduction in the enzyme activity, biomass, pellet diameter, and dissolved oxygen concentration. Calculations using the Brown equation showed that the maximum and average shear rates during mixing with the FZ impeller were lower than that when using the DRT. Therefore, the use of an FZ impeller, particularly at low Phi, enhanced the cultivation of A. oryzae. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.SOC BIOSCIENCE BIOENGINEERING JAPAN, 2017年01月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 123(1) (1), 101 - 108, 英語[査読有り]研究論文（学術雑誌）
- 2017年, Journal of Bioprocessing & Biotechniques, 7(2) (2), 300: 1 - 5, 英語[査読有り]研究論文（学術雑誌）
- The expression of epidermal growth factor receptor (EGFR) across a wide range of tumor cells has attracted attention for use as a tumor marker in drug delivery systems. Therefore, binding molecules with the ability to target EGFR have been developed. Among them, we focused on affibodies that are binding proteins derived from staphylococcal protein A. By displaying affibody (Z(EGFR)) binding to EGFR on the surface of a bio-nanocapsule (BNC) derived from a hepatitis B virus (HBV), we developed an altered BNC (Z(EGFR)-BNC) with a high specificity to EGFR-expressing cells. We considered two different types of Z(EGFR) (Z955 and Z1907), and found that the Z1907 dimer-displaying BNC ([Z1907 or [Bmim][BF(4)] biphasic systems following a 24h reaction. While lipases are known to be severely deactivated by an excess amount of methanol (e.g. 1.5 Mequiv. of methanol against oil) in a conventional system, methanolysis successfully proceeded even with a methanol/oil ratio of 4 in the ionic liquid biphasic system, where the ionic liquids would work as a reservoir of methanol to suppress the enzyme deactivation. When only w-ROL was used as a biocatalyst for methanolysis, unreacted monoglyceride remained due to the 1,3-positional specificity of R. oryzae lipase. High ME conversion was attained by the combined use of two types of whole-cell biocatalysts, w-ROL and r-mdlB. In a stability test, the activity of w-ROL was reduced to one-third of its original value after incubation in [Bmim][BF(4)] for 72 h. The stability of w-ROL in [Bmim][BF(4)] was greatly enhanced by cross-linking the biocatalyst with glutaraldehyde. The present Study demonstrated that ionic liquids are promising candidates for use as the second solvent in biodiesel fuel production by whole-cell biocatalysts. (C) 2009 Elsevier Inc. All rights reserved.ELSEVIER SCIENCE INC, 2010年01月, ENZYME AND MICROBIAL TECHNOLOGY, 46(1) (1), 51 - 55, 英語[査読有り]研究論文（学術雑誌）
- In order to achieve direct fermentation of an optically pure d-lactic acid from cellulosic materials, an endoglucanase from a Clostridium thermocellum (CelA)-secreting plasmid was introduced into an l-lactate dehydrogenase gene (ldhL1)-deficient Lactobacillus plantarum (a dagger ldhL1) bacterial strain. CelA expression and its degradation of beta-glucan was confirmed by western blot analysis and enzyme assay, respectively. Although the CelA-secreting a dagger ldhL1 assimilated cellooligosaccharides up to cellohexaose (although not cellotetraose), the main end product was acetic acid, not lactic acid, due to the conversion of lactic acid to acetic acid. Cultivation under anaerobic conditions partially suppressed this conversion resulting in the production of 1.27 g/l of D-lactic acid with a high optical purity of 99.5% from a medium containing 2 g/l of cellohexaose. Subsequently, D-lactic acid fermentation from barley beta-glucan was carried out with the addition of Aspergillus aculeatus beta-glucosidase produced by recombinant Aspergillus oryzae and 1.47 g/l of D-lactic was produced with a high optical purity of 99.7%. This is the first report of direct lactic acid fermentation from beta-glucan and a cellooligosaccharide that is a more highly polymerized sugar than cellotriose.SPRINGER, 2010年01月, Applied Microbiology and Biotechnology, 85(3) (3), 643 - 650, 英語[査読有り]研究論文（学術雑誌）
- Lactic acid (LA) is an important and versatile chemical that can be produced from renewable resources such as biomass. LA is used in the food, pharmaceutical, and polymers industries and is produced by microorganism fermentation; however, most microorganisms cannot directly utilize biomass such as starchy materials and cellulose. Here, we summarize LA production using several kinds of genetically modified microorganisms, such as LA bacteria, Escherichia coli, Corynebacterium glutamicum, and yeast. Using gene manipulation and metabolic engineering, the yield and optical purity of LA produced from biomass has been significantly improved. In this review, the drawbacks as well as improvements of LA production by fermentation is discussed.SPRINGER, 2010年01月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85(3) (3), 413 - 423, 英語[査読有り]研究論文（学術雑誌）
- The production of optically pure D-lactic acid via xylose fermentation was achieved by using a Lactobacillus plantarum NCIMB 8826 strain whose L-lactate dehydrogenase gene was deficient and whose phosphoketolase genes were replaced with a heterologous transketolase gene. After 60 h of fermentation, 41.2 g/liter of D-lactic acid was produced from 50 g/liter of xylose.AMER SOC MICROBIOLOGY, 2009年12月, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(24) (24), 7858 - 7861, 英語[査読有り]研究論文（学術雑誌）
- Bionanocapsule (BNC) is hollow nanoparticle composed of the l-protein of the hepatitis B virus surface antigen. BNC allows targeted delivery of either genes or drugs only to hepatocytes, but not to other cell types. In this study, we attempted to alter the specificity of BNC by insertion of biotin-acceptor peptide (BAP), which is efficiently biotinylated using biotin ligase BirA from Escherichia coli. Using streptavidin as a linker, biotinylated BNC could be display various biotinylated ligands that are otherwise difficult to fuse with BNC, such as antibodies, synthetic peptides and functional molecules. BAP-fused BNC was efficiently biotinylated and effectively displayed streptavidin. Furthermore, we demonstrated that biotinylated BNC was internalized into targeted cells via biotinylated Nanobody displayed on the BNC surface. Biotinylated BNC permit display of diverse ligands, and thus have potential as a versatile carrier for drug delivery to a variety of target cells.OXFORD UNIV PRESS, 2009年12月, JOURNAL OF BIOCHEMISTRY, 146(6) (6), 867 - 874, 英語[査読有り]研究論文（学術雑誌）
- The effect of the reaction conditions on the ester synthesis reaction with CALB displaying yeast whole cells was determined. Utilization of hydrophobic organic solvent improved the efficiency of the ester synthesis reaction. Also the initial water content was important for the expression of the ester synthesis activity of CALB displaying yeast whole cells. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(5) (5), 369 - 371, 英語[査読有り]研究論文（学術雑誌）
- Optically pure D-lactic acid fermentation from arabinose was achieved by using the Lactobacillus plantarum NCIMB 8826 strain whose L-lactate dehydrogenase gene was deficient and whose phosphoketolase gene was substituted with a heterologous transketolase gene. After 27 h of fermentation, 38.6 g/liter of D-lactic acid was produced from 50 g/liter of arabinose.AMER SOC MICROBIOLOGY, 2009年08月, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(15) (15), 5175 - 5178, 英語[査読有り]研究論文（学術雑誌）
- beta-Glucosidase (BGL1) from Aspergillus oryzae was efficiently produced in recombinant A. oryzae using sodM promoter-mediated expression system. The yield of BGL1 was 960 mg/l in liquid culture, which is 20-fold higher than the yield of BGL1 produced using the yeast Saccharomyces cerevisiae. Recombinant BGL1 converted isoflavone glycosides into isoflavone aglycones more efficiently than P-glucosidase from almond. In addition, BGL1 produced isoflavone aglycones even in the presence of the insoluble form of isoflavone glycosides. (C) 2008 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, 2009年08月, Journal of Molecular Catalysis B: ,, 59(4) (4), 297 - 301, 英語[査読有り]研究論文（学術雑誌）
- A molecular display technology that uses the displayed proteins on cell surfaces has many applications in microbiology and molecular biology. Here, we describe the resistance of displayed proteins to proteases using simulated gastric fluid (SGF), which included pepsin at pH 2. The displayed beta-glucosidase resisted pepsin digestion compared with secreted, free beta-glucosidase. In SDS-PAGE and Western blotting analysis, the secreted beta-glucosidase was immediately digested within 1 min following SGF treatment, although the displayed beta-glucosidase was stable for more than 60 min following SGF treatment. In addition, the residual activity of secreted beta-glucosidase was completely destroyed after 10 min SGF treatment. However, displayed beta-glucosidase retained 14% of its residual activity following the same treatment. These results clearly show that cell surface display technology using enzymes can reveal the protease resistance of a protein of interest under various conditions.SPRINGER, 2009年08月, BIOTECHNOLOGY LETTERS, 31(8) (8), 1259 - 1264, 英語[査読有り]研究論文（学術雑誌）
- DNA aptamers, which bind specific molecule, such as 8-OHdG, with high affinity were investigated using an in vitro selection strategy called systematic evolution of ligands by exponential enrichment (SELEX). However, 8-OHdG was difficult to immobilize on a carrier for SELEX. Therefore, a DNA aptamer binding to 8-OHdG was selected using GMP-agarose as an analogue from a library of about 4(60) random ssDNA sources. As a result, three aptamer candidates were selected. Among the selected DNA aptamers, the No. 22 DNA aptamer exhibited a high affinity for 8-OHdG. The dissociation constant, K(D), of No. 22 DNA aptamer was on the order of 0.1 mu mol/L. This result suggests that using an analogue will be a useful new SELEX method for obtaining various aptamers that are difficult to immobilize on a matrix. (C) 2009 Elsevier Ltd. All rights reserved.PERGAMON-ELSEVIER SCIENCE LTD, 2009年07月, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 19(13) (13), 3619 - 3622, 英語[査読有り]研究論文（学術雑誌）
- We constructed a novel cell surface display system to control the ratio of target proteins on the Saccharomyces cerevisiae cell surface, using two pairs of protein-protein interactions. One protein pair is the Z domain of protein A derived from Staphylococcus aureus and the Fc domain of human immunoglobulin G. The other is the cohesin (Coh) and dockerin (Dock) from the cellulosome of Clostridium cellulovorans. In this proposed displaying system, the scaffolding proteins (fusion proteins of Z and Coh) were displayed on the cell surface by fusing with the 3' half of alpha-agglutinin, and the target proteins fused with Fc or Dock were secreted. As a target protein, a recombinant Trichoderma reesei endoglucanase II (EGII) was secreted into the medium and immediately displayed on the yeast cell surface via the Z and Fc domains. Display of EGII on the cell surface was confirmed by hydrolysis of beta-glucan as a substrate, and EGII activity was detected in the cell pellet fraction. Finally, two enzymes, EGII and Aspergillus aculeatus beta-glucosidase 1, were codisplayed on the cell surface via Z-Fc and Dock-Coh interactions, respectively. As a result, the yeast displaying two enzymes hydrolyzed beta-glucan to glucose very well. These results strongly indicated that the proposed strategy, the simultaneous display of two enzymes on the yeast cell surface, was accomplished by quantitatively controlling the display system using affinity binding.AMER SOC MICROBIOLOGY, 2009年06月, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(12) (12), 4149 - 4154, 英語[査読有り]研究論文（学術雑誌）
- To allow the comprehensive assessments of yeast expression systems, a simple and immediate method for simultaneously evaluating the expression level and plasmid maintenance in yeast was demonstrated. This method uses green fluorescent protein (GFP) and flow cytometry (FCM) and is characterized by a dual analysis of the average intensity of GFP fluorescence and the population of GFP-expressing cells. The FCM analysis of GFP fluorescence intensity rapidly quantifies the expression level without complex manipulations, such as the enzymatic reaction of a lacZ reporter assay. Moreover, the single-cell analysis revealed that the proportion of cells expressing GFP in the cell cluster reflects the plasmid retention rate; therefore, the FCM analysis of the GFP-expressing population allows the immediate estimation of the plasmid retention rate without the 2- or 3-day incubation required for colony counting. We show that the FCM analysis with GFP reporter is a suitable method to explore the hopeful expression vector and host strain or establish the several expression systems exhibiting the characteristic properties in yeast.OXFORD UNIV PRESS, 2009年06月, JOURNAL OF BIOCHEMISTRY, 145(6) (6), 701 - 708, 英語[査読有り]研究論文（学術雑誌）
- The goal of this research was to construct a stable and efficient process for the production of ethanol from raw starch, using a recombinant Saccharomyces cerevisiae. which is productive even under conditions such as non-selection or long-term operation. Three recombinant yeast strains were used, two haploid strains (MT8-1SS and NBRC1440SS) and one diploid strain (MN8140SS). The recombinant strains were constructed by integrating the glucoamylase gene from Rhizopus oryzae fused with the 3'-half of the alpha-agglutinin gene as the anchor protein, and the alpha-amylase gene from Streptococcus bovis, respectively, into their chromosomal DNA by homologous recombination. The diploid strain MN8140SS was constructed by mating these opposite types of integrant haploid strains in order to enhance the expression of integrated amylase genes. The diploid strain had the highest ethanol productivity and reusability during fermentation from raw starch. Moreover, the ethanol production rate of the integrant diploid strain was maintained when batch fermentation was repeated three times (0.67. 0.60, and 0.67 g/l/h in each batch). These results clearly show that a diploid strain developed by mating two integrant haploid strains is useful for the establishment of an efficient ethanol production process. (C) 2009 Elsevier Inc. All rights reserved.ELSEVIER SCIENCE INC, 2009年05月, Enzyme and Microbial Technology, 44(5) (5), 344 - 349, 英語[査読有り]研究論文（学術雑誌）
- Bionanocapsule (BNC) is a hollow nanoparticle composed of L-protein of the hepatitis B virus surface antigen. BNC can deliver genes or drugs into specific human hepatocytes, but delivery is limited to hepatocytes. In this study, we attempted to alter the specificity of BNCs by genetically introducing cell-penetrating peptides (CPPs), such as arginine-rich peptides, into BNCs. The CPP-fused BNC was efficiently internalized into various cell lines in a short period without significant cytotoxicity. These results show that CPP-BNC could be applied as an efficient carrier for gene and drug delivery. (C) 2009 Elsevier Ltd. All rights reserved.PERGAMON-ELSEVIER SCIENCE LTD, 2009年03月, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 19(5) (5), 1473 - 1476, 英語[査読有り]研究論文（学術雑誌）
- A Candida antarctica lipase B (CALB)-displaying yeast whole-cell biocatalyst was constructed with the integration of the CALB cell-surface display expression cassette in the yeast genome and cell fusion by mating. Lipase hydrolytic activity of the yeast whole-cell biocatalyst subsequently increased, in both a- and alpha-type yeast cells, with the number of copies of the CALB cell-surface display expression cassette introduced, and reached 43.6 and 32.2 U/g-dry cell at 168 h cultivation, respectively. The lipase hydrolytic activity of whole cells in diploid yeast cells containing eight copies of the CALB cell-surface expression cassette reached 117 U/g-dry cell, and this value is approximately ninefold higher than that of the previously reported haploid CALB cell-surface displaying yeast using a multi-copy plasmid (Tanino et al. Appl. Microbial Biotechnol 75:1319-1325, 2007). This improved novel CALB-displaying yeast whole-cell biocatalyst could repeatedly catalyze the polyester, polybutylene adipate, synthesis reaction, using adipic acid and 1, 4-butandiol as the monomer molecules, four times in succession. This is the first report of the polymer synthesis using enzyme displaying yeast as the catalyst. The ratios of cyclic compounds in the polybutylene adipates synthesized with the CALB-displaying yeast whole-cells were lower than that in the polybutylene adipate synthesized with conventional metal catalysis. From these results, it appears that the use of CALB-displaying yeast cells could be useful for the polyester synthesis reaction, with reduced by-product production.SPRINGER, 2009年02月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 82(1) (1), 59 - 66, 英語[査読有り]研究論文（学術雑誌）
- 公益社団法人 化学工学会, 2009年, 化学工学会 研究発表講演要旨集, 2009, 728 - 728, 日本語
- 公益社団法人 化学工学会, 2009年, 化学工学会 研究発表講演要旨集, 2009, 727 - 727, 日本語
- 公益社団法人 化学工学会, 2009年, 化学工学会 研究発表講演要旨集, 2009, 601 - 601, 日本語
- 平成14年度に日本政府の総合戦略「バイオマス・ニッポン」が策定され、バイオマスの有効利用による持続的に発展可能な社会の実現が提言されている。この戦略は、（1）バイオマスの有効利用に基づく地球温暖化防止や循環型社会形成の達成、（2）日本独自のバイオマス利用法の開発による戦略的産業の育成を目指すものである。また、地球規模での環境保護の観点から、バイオマス原料は日本のみならず、世界中から安価かつ豊富な資源の積極的な利用が求められている。さらに石油資源枯渇や価格高騰の影響より、これまでの石油資源依存型の「オイルリファイナリー」から、バイオマスベースの「バイオリファイナリー」社会への転換が急務とされている。米国においても、1999年にクリントン大統領が「バイオ製品およびバイオマスエネルギーの開発・普及に関する大統領令」を発令し、バイオマスエネルギーの利用率を2010年までに10％までに拡大する方針を指示し、ヨーロッパにおいても同様の方針が打ち出されている状況である。このような社会的背景も重なり、現在、グルコースから生産されるバイオエタノールはブラジル、米国をはじめとして多くの地域でガソリンと混合され、自動車用燃料として利用されている。しかしながら、食料価格高騰の問題原因ともなっており、代替原料が模索されている。木質系バイオマスはデンプンよりもはるかに豊富に存在し、また古紙や廃材、農産廃棄物など処理が問題化しているものを利用するため原料が安価であることなどから、将来の石油代替エネルギー資源として特に有望視されている。日本草地学会, 2009年, 日本草地学会誌, 55(3) (3), 270 - 273, 日本語
- Titanium dioxide (TiO_2) is known as sonocatalyst which generate hydroxyl radicals when the ultrasonic (US) is irradiated to TiO_2 (TiO_2/ US method). In this study, we investigated the cell damage effect and antitumor effect by the combination of ultrasonic irradiation and TiO_2 nanoparticles that is modified with preSl/S2 protein for targeting HepG2 cells. For irradiating of US to the preS1/S2-TiO_2 nanoparticles incorporated HepG2, cell injuring efficiency was evaluated by Trypan blue dye exclusion test. As a result, the cytostatic activity was acquired when irradiated at 1MHz, 0.1 W/ cm^2, 30 second, 50% duty in the presence of TiO_2 nanoparticle modified with preS1/S2 protein. This cytostatic activity was observed at 24 hour after US irradiation, and this effect was significantly enhanced as time elapsed. Also, antitumor effect by TiO_2 / U.S. method in vivo was evaluated. When ultrasonic irradiation was performed by the frequency of 3 times per week, and the irradiation conditions of 1 MHz, 1 W/cm^2, 1 min, no significant damages to the mice was ovserved. These irradiation condition revealed the antitumor effect significantly. Moreover the further research, such as the examination of the irradiation conditions of ultrasound, injection amount of TiO_2 nanoparticles and the specific binding ability to HepG2 tumor after i.v. injection, are proposed.日本ソノケミストリー学会, 2009年, ソノケミストリー討論会講演論文集, 18, 5 - 6, 日本語
- 公益社団法人 化学工学会, 2009年, 化学工学会 研究発表講演要旨集, 2009, 912 - 912, 日本語
- 公益社団法人 化学工学会, 2009年, 化学工学会 研究発表講演要旨集, 2009, 242 - 242, 日本語
- Bionanocapsules (BNCs) are nanoparticles with a high biocompatibility composed of the L protein of the hepatitis B virus surface antigen. BNC can deliver bioactive molecules to hepatocytes efficiently and specifically. However, delivery is limited to hepatocytes and incorporation of proteins into BNC is quite troublesome. Here, in order to alter the specificity of BNC and to achieve efficient protein delivery, we developed engineered BNC displaying the ZZ domain of protein A and incorporating enhanced green fluorescent protein (EGFP) inside the particles using an insect cell expression system. The ZZ domain displayed on the surface of BNC binds to anti-epidermal growth factor receptor (EGFR) antibodies, allowing specific delivery of EGFP to HeLa cells. The engineered BNCs are a promising and powerful tool for efficient and cell-specific protein delivery.OXFORD UNIV PRESS, 2008年12月, JOURNAL OF BIOCHEMISTRY, 144(6) (6), 701 - 707, 英語[査読有り]研究論文（学術雑誌）
- A novel cell-surface display system was constructed in Aspergillus oryzae. Each of the five genes encoding the putative cell-wall-localized protein from the A. oryzae genome was cloned and these cell-surface anchor functions were examined by fusion to the C-terminal of the green fluorescent protein (GFP). Using the MP1 and CWP proteins as anchor proteins, GFP signals were strongly observed on the cell surface of recombinant A. oryzae. When these proteins were used as anchor proteins for cell-surface display of beta-glucosidase from A. oryzae, enzyme activity was detected on the cell surface. In particular, beta-glucosidase activity of recombinant A. oryzae using MP1, a putative glycosylphosphatidylinositol (GPI) anchor protein was higher than CWP. Based on these results, it was concluded that the MP1 protein can act as a GPI-anchor protein in A. oryzae, and the proposed cell-surface display system using MP1 allows for the display of heterogeneous and endogenous proteins.SPRINGER, 2008年12月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 81(4) (4), 711 - 719, 英語[査読有り]研究論文（学術雑誌）
- The generation of hydroxyl (CH) radicals was investigated during ultrasonic irradiation and in the presence of TiO(2). The effect of TiO(2) on an ultrasonic system's oxidation power was evaluated by examining the oxidation of salicylic acid. The generation of the salicylic acid derivatives, 2,3-dihydroxybenzoic acid (DHBA) and 2,5-DHBA, was measured by high-performance liquid chromatography coupled with electro-chemical detection under different experimental conditions. The presence of TiO(2) enhanced the generation of DHBA during ultrasonic irradiation, thus indicating a higher oxidation power in the ultrasonic system. Al(2)O(3) also increased the generation of DHBA during irradiation; however, the effect of TiO(2) was found to be higher than that of Al(2)O(3). The addition of OH radical scavengers such as dimethylsulfoxide (DMSO), methanol and mannitol significantly suppressed the production of DHBA, and DMSO was found to have the highest suppressive effect among all scavengers. The effects of dissolved gases on the generation of OH radicals were further studied, and their power was found to be in the order Xe > At > O(2) > N(2). The degassing of the irradiation solution completely suppressed the generation of CH radicals. These results indicate that the presence of TiO(2) accelerates the generation of OH radicals during ultrasonic irradiation, and that the process may be mediated through the induction of cavitation bubbles in irradiating solutions. (c) 2008 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, 2008年09月, ULTRASONICS SONOCHEMISTRY, 15(6) (6), 988 - 994, 英語[査読有り]研究論文（学術雑誌）
- The yeast Saccharomyces cerevisiae GRI-117-UK was transformed to either display or secrete beta-glucosidase 1 (BGL1) from the koji mold, Aspergillus oryzae. The P-glucosidase activity of BGL1-displaying yeast strains reached 405.9 U/g dry cell mass after 72 h of cultivation in YPD medium. The optimal pH and temperature for BGL1 displayed on the cell surfaces of the yeast were 5.0 and 55 degrees C, while the optima for BCL1 secreted by the yeast were 4.0 and 55 degrees C. The displayed BGL1 was stable at higher pH compared with the secreted BGL1. In addition, the thermostability of BGL1 was improved by displaying the enzyme on the yeast cell surfaces. In addition, the displayed and secreted forms of BGL1 had similar substrate specificity. beta-Glucosidase hydrolyzes daidzin and genistin, which are the glycoside forms of soybean isoflavones, to the aglycones. Isoflavone aglycones were efficiently produced by BGL1-displaying yeast from an isoflavone mixture: at optimal temperature and pH the rate of aglycone production was at least 15.8 g/(1h). After 144 h of reaction, almost isoflavones were converted to its aglycone by BGL1-displaying yeast. The results of the present study demonstrate that BGL1-displaying yeast strains are effective whole cell biocatalysts of isoflavone aglycone production. (c) 2008 Elsevier B. V. All rights reserved.ELSEVIER SCIENCE BV, 2008年09月, Journal of Molecular Catalysis B, 55(1-2) (1-2), 69 - 75, 英語[査読有り]研究論文（学術雑誌）
- 2008年09月, Proceedings of the 10th International Chemical and Biological Engineering Conference-CHEMPOR 2008, Braga, Portugal (on CD-ROM), E20, 4pages, 英語Bioethanol Production from Sugars by Yeast Having Sugar Uptake Ability by Expression of Sugar Transporter[査読有り]研究論文（学術雑誌）
- 2008年06月, Colloids and Surfaces B: Biointerfaces, Vol 64, pp. 10-15, 英語Properties of TiO2-Polyacrylic acid (PAA) Dispersions having Molecular Recognition Ability[査読有り]研究論文（学術雑誌）
- Here, we describe a yeast-based fluorescence reporter assay for G protein-coupled receptor (GPCR) signalling using a flow cytometer (FCM). The enhanced green fluorescent protein (EGFP) gene was integrated into the FUS1 locus as a reporter gene. The engineered yeast was able to express the EGFP in response to ligand stimulation. Gene-disrupted yeast strains were constructed to evaluate the suitability of the yeast-based fluorescence screening system for heterologous GPCR. When receptor was expressed by episomal plasmid, the proportion of the signalling-activated cells in response to ligand stimulation decreased significantly. The GPCR-signalling-activated and non-activated cell clusters were individually isolated by analysing the fluorescence intensity at the single-cell level with FCM, and it was found that the plasmid retention rate decays markedly in the non-activated cell cluster. We attributed the loss of plasmid to G1 arrest in response to signalling, and successfully improved the plasmid retention rate by disrupting the FAR1 gene and avoiding cell cycle arrest. Our system will be a powerful tool for the quantitative and high-throughput GPCR screening of yeast-based combinatorial libraries using FCM. © 2008 The Japanese Biochemical Society.2008年05月, Journal of Biochemistry, 143(5) (5), 667 - 674, 英語[査読有り]研究論文（学術雑誌）
- For efficient production of isoflavone aglycones from soybean isoflavones, we isolated three novel types of beta-glucosidase (BGL1, BGL3, and BGL5) from the filamentous fungi Aspergillus oryzae. Three enzymes were independently displayed on the cell surface of a yeast Saccharomyces cerevisiae as a fusion protein with alpha-agglutinin. Three beta-glucosidase-displaying yeast strains hydrolyzed isoflavone glycosides efficiently but exhibited different substrate specificities. Among these beta-glucosidases, BGL1 exhibited the highest activity and also broad substrate specificity to isoflavone glycosides. Although glucose released from isoflavone glycosides are generally known to inhibit beta-glucosidase, the residual ratio of isoflavone glycosides in the reaction mixture with BGL1-displaying yeast strain (Sc-BGL1) reached approximately 6.2%, and the glucose concentration in the reaction mixture was maintained at lower level. This result indicated that Sc-BGL1 assimilated the glucose before they inhibited the hydrolysis reaction, and efficient production of isoflavone aglycones was achieved by engineered yeast cells displaying beta-glucosidase.SPRINGER, 2008年05月, Applied Microbiology and Biotechnology, 79(1) (1), 51 - 60, 英語[査読有り]研究論文（学術雑誌）
- The pretreatment of TiO2-photocatalysts in solutions of H2O2 was studied by examining the decolorization of methylene blue in the dark. Incubation of TiO2 particles in H2O2 solutions increased the oxidizing capacity of TiO2. Methylene blue (0.3 mM) was degraded in the presence of pretreated TiO2, and a decolorizing ratio of 47% was obtained after a 48-h incubation period in the presence of 5.0 g/L pretreated TiO2. Titanium peroxide as a stable oxidant, which can be synthesized with the reaction of titanium sulfate and H2O2, was studied in the decolorizing process of methylene blue. Concentrations of methylene blue were significantly reduced in the presence of titanium peroxide, and a greater extent of decolorization was obtained with larger amounts of titanium peroxide. A 63% decrease in methylene blue concentration was achieved in 5 h incubation in the presence of 4.0 g/L titanium peroxide. H2O2 accelerated the decolorizing process in the presence of titanium peroxide. The addition of 100 mM H2O2 to a methylene blue solution containing 2.0 g/L titanium peroxide increased the decolorizing ratio to 85% after 5 h incubation. The addition of a hydroxyl radical scavenger, dimethyl sulfoxide, significantly decreased the decolorizing ratio, indicating the role of hydroxyl radicals in the oxidation process. (c) 2007 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, 2008年05月, JOURNAL OF HAZARDOUS MATERIALS, 153(1-2) (1-2), 551 - 556, 英語[査読有り]研究論文（学術雑誌）
- 公益社団法人 化学工学会, 2008年, 化学工学会 研究発表講演要旨集, 2008, 328 - 328, 日本語
- 公益社団法人 化学工学会, 2008年, 化学工学会 研究発表講演要旨集, 2008, 990 - 990, 日本語
- To well characterize disinfection system and to investigate the mechanisms of the cell killing by TiO_2/U.S. method, Escherichia coli (NOVABLUE strain) was used as a model and target microorganism. Our previous studies reported that ultrasonic irradiation was very effective to kill the microbial cells. Microbial cells are physically and chemically sterilized by ultrasonic irradiation. Our present study was under taken, to investigate a suitable mechanism for cells killing and to develop the sterilization effect using electrochemically under U.S. in the presence of TiO_2. We suggested that, toxic substances such as free OH radical generated by electrolysis have been shown to be responsible for the sterilization effect.日本ソノケミストリー学会, 2008年, ソノケミストリー討論会講演論文集, 17, 16 - 17, 日本語
- Strong bacteriocidal activity of titanium dioxide (TiO_2) irradiated with ultrasound (U.S.) is an established phenomenon. A new non-woven TiO_2 fabric under U.S. system was used to well characterize the sonocatalytic disinfection and lipid peroxidation of Escherichia coli (E. coli) cells membrane and to investigate the possible killing mechanisms of its cell. It was found that applying ultrasonic irradiation with non-woven TiO_2 fabrics reduces the E. coli cell concentration very rapidly, which indicated very high disinfection rate at this system. Diphenyl-1-pyrenylphosphin (DPPP) was used as a non-fluorescent probe for lipid peroxidation on E. coli cells membrane. Reaction of DPPP with U.S. treated samples and organic hydroperoxides were examined by monitoring increase in fluorescence intensity of DPPP oxide (DPPP=O). The mechanism of cell killing and lipid peroxidation were further investigated by examining the effect of OH radical scavenger. The scavenger studies indicated the functional role of hydroxyl (OH) radicals in the initiation of ultrasound induced lipid peroxidation.日本ソノケミストリー学会, 2008年, ソノケミストリー討論会講演論文集, 17, 48 - 49, 英語
- Effects of TiO_2 particles addition on OH radical formation by ultrasonic irradiation were investigated through comparison of Al_2O_3 particles. Formation of cavitation bubbles is accelerated on the surface of particles because sonoluminescence intensity and white noise intensity are increased by particle addition. It is suggested that there is the close relationship between the effeciency of TiO_2 on OH radical formation and cavitation phenomena. Irradiation frequency is the very important factor for cavitation phenomena. In this reseach, the effects of frequency changes on OH radical formation by ultrasonic irradiation were investigated in the presence of TiO_2.日本ソノケミストリー学会, 2008年, ソノケミストリー討論会講演論文集, 17, 50 - 51, 日本語
- Our previous study demonstrated that the titanium dioxide (TiO_2) could catalyze production of hydroxyl (OH) radicals by ultrasound (U.S.) irradiation even without any photo-excitation. The purpose of this study was to extend the applicability of our "TiO_2/U.S. system" to drug delivery systems for cancer therapy by modifying the surface of TiO_2 nanoparticles with PreS1/S2 protein for targeting HepG2 cells. To evaluate the specific binding of the preS1/S2-conjugated TiO_2 particles (preS1/S2-TiO_2), liver and colon cancer cell lines, HepG2 and WiDr were incubated with the preS1/S2-TiO_2 (0.01% (v/v)) for 6 hours and subjected to fluorescent immunostaining with anti-preS1 antibody. The fluorescence was detected only in the case of HepG2 cells, indicating specific binding and incorporation of preS1/S2-TiO_2. To evaluate the sonocatalytic cytotoxicity of HepG2 cells with incorporation of preS1/S2-TiO_2, the culture dish was irradiated with U.S. at the frequency of 1MHz and with output power of 0.3 W/m^2 for 30 sec (50% duty). Compared to the control condition without preS1/S2-TiO_2 incorporation and/or U.S. irradiation, the growth of HepG2 cells was restrained with both the preS1/S2-TiO_2 incorporation and the U.S. irradiation at initial time of culture, suggesting our "TiO_2/U.S. system" could be promising tool for cancer therapy.日本ソノケミストリー学会, 2008年, ソノケミストリー討論会講演論文集, 17, 96 - 97, 日本語
- 公益社団法人 化学工学会, 2008年, 化学工学会 研究発表講演要旨集, 2008, 380 - 380, 日本語
- 公益社団法人 化学工学会, 2008年, 化学工学会 研究発表講演要旨集, 2008, 381 - 381, 日本語
- 公益社団法人 化学工学会, 2008年, 化学工学会 研究発表講演要旨集, 2008, 348 - 348, 日本語
- 公益社団法人 化学工学会, 2008年, 化学工学会 研究発表講演要旨集, 2008, 225 - 225, 日本語
- 2008年, Appl. Microbiol. Biotechnol., 79: 51-60, 英語Growth acceleration of plants and mushroom by erythritol[査読有り]
- Polyelectrolyte polyacrylic acid (PAA), used in the chemical modification of titanium dioxide (TiO2) nanoparticles, allows TiO2 nanoparticles to remain in suspension at neutral pH. The anti-17 beta-estradiol (E2) antibody was immobilized on PAA-modified TiO2 (PAA-TiO2) nanoparticles via covalent bonding between the carboxylic acid of PAA and the amino group of the antibody. The anti- E2-antibody-immobilized TiO2 (E2Ab-PAA-TiO2) nanoparticles can form a suspension at neutral pH, with a particle size of less than 100 nm. The E2-PAA-TiO2 nanoparticles caused the photocatalytic degradation of a typical TiO2 substrate, methylene blue. The anti-E2 antibody immobilized on the TiO2 surface recognized and bound E2 in the solution, thereby improving the efficiency of E2 degradation compared with that of PAA-TiO2 nanoparticles. These results demonstrate that the E2Ab-PAA-TiO2 nanoparticles developed in this study can be used in water treatment technology. Furthermore, this strategy of immobilizing proteins on nanoscale TiO2 particles creates new applications not only in the treatment of environmental waste, but also in medical and public sanitation processes.SOC BIOSCIENCE BIOENGINEERING JAPAN, 2007年10月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 104(4) (4), 339 - 342, 英語[査読有り]研究論文（学術雑誌）
- Using biomass support particles (BSPs) as a cell immobilized matrix, immobilized recombinant Streptomyces lividans continuously produced phospholipase D (PLD) in a yield of about 1.5 x 10(4) U/L in each of eight batches. In contrast to the original strain Streptoverticillium cinnamoneum, this heterologous expression system with an immobilization method is capable of producing secretory PLD with an 8-fold greater efficiency. The presence of both glucose and tryptone in the initial culture medium also promoted secretory production, and PLD activity around 3.0 x 10(4) U/L were achieved. In addition, the promoter region of PLD ORF was deduced, and three types of plasmid having different lengths of promoter sequence were constructed. The deduced sequence had same effect on either of PLD production or mycelium immobilization, and the transformants harboring each of three plasmids showed the similar cultivation profiles (3.0 x 10(4) U/L). A combination of the immobilization method with BSPs and S. lividans transformant harboring the deduced plasmid has the potential for producing secretory PLD in the culture supernatant continuously. (c) 2007 Elsevier Inc. All rights reserved.ELSEVIER SCIENCE INC, 2007年07月, ENZYME AND MICROBIAL TECHNOLOGY, 41(1-2) (1-2), 156 - 161, 英語[査読有り]研究論文（学術雑誌）
- The gene that encodes phospholipase D (PLD) from Streptoverticillium cinnamoneum contains three consensus regions (Region 1, 11 and Was shown in Fig. 1A) that are conserved among the PLD superfamily. The glycine-glycine (GG) motif in Region I and the glycine-serine (GS) motif in Region IV are also conserved in the PLD superfamily. These (GG and GS) motifs are located 7 residues downstream from each HKD motif. In an investigation of fifteen GG/GS motif mutants, generated as fusion proteins with maltose-binding protein (MBP-PLDs), three highly active mutants were identified. Three of the mutants (G215S, G216S, and G216S-S489G) contained a serine residue in the GG motif, and exhibited approximately a 9-27-fold increased transphosphatidylation activity to DPPC compared with recombinant wild type MBP-PLD. When heat stability was compared between three mutants and the recombinant wild type, only G216S-S489G showed heat labile properties. It appears that the 489th serine residue in the GS motif also contributes to the thermal stability of the enzyme. In addition, the GG/GS motif was very close to the active center residue, including two HKD motifs, as shown by computer modeling. The findings suggest that the GG/GS motif of PLD is a key motif that affects catalytic function and enzymatic stability. (c) 2007 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, 2007年06月, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1774(6) (6), 671 - 678, 英語[査読有り]研究論文（学術雑誌）
- A series of experiments were carried out to study the degradation of methylene blue by the irradiation of ultrasound onto TiO2 in aqueous solution. A statistically significant decrease in the concentration of methylene blue was observed after 60 min irradiation. While the reduction was 22% of the initial concentration without H2O2, addition of H2O2 significantly enhanced the degradation of methylene blue for the TiO2 containing system (85% reduction of the initial concentration). The addition of H2O2 had no effect on the methylene blue degradation when the system contained Al2O3. The degradation ratio of methylene blue was dependent on the amount of TiO2 and also the specific surface area of TiO2 in the solution. The effects of radical scavenging agents on the degradation of methylene blue were also investigated for the system with TiO2. It was found that the radical scavenging agents dimethyl sulfoxide (DMSO), methanol, and mannitol suppressed the degradation, with DMSO being the most effective. The effect of pH on the degradation of methylene blue was further investigated. An U-shaped change in the concentration of methylene blue in the presence of TiO2 was observed along with the change in pH values (pH 3-12), and the highest degradation ratio was observed at around pH 7. In conclusion, ultrasound irradiation of TiO2 in aqueous solution resulted in significant generation of hydroxyl radicals, and this process may have potential for the treatment of organic dyes in wastewater. (C) 2006 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, 2007年02月, ULTRASONICS SONOCHEMISTRY, 14(2) (2), 184 - 190, 英語[査読有り]研究論文（学術雑誌）
- 日本ソノケミストリー学会, 2007年, ソノケミストリー討論会講演論文集, 2007, 50 - 51, 英語
- 公益社団法人 化学工学会, 2007年, 化学工学会 研究発表講演要旨集, 2007, 391 - 391, 日本語
- 日本ソノケミストリー学会, 2007年, ソノケミストリー討論会講演論文集, 2007, 102 - 102, 英語
- 公益社団法人 化学工学会, 2007年, 化学工学会 研究発表講演要旨集, 2007, 353 - 353, 日本語
- An ultrasonic system with TiO2 particles was utilized for disinfection of Escherichia coli (E coli) under condition of darkness. The mechanism of disinfection was studied by investigating the cell lysis and degradation of DNA leaked through the cell membrane. The degradation of DNA was enhanced remarkably in the presence of TiO2 particles. The effects of Al2O3 particles on the degradation of DNA were also examined, and these particles were found to be less effective than TiO2 particles at enhancing degradation. Compared with TiO2 addition case, the ultrasonic irradiation with no particles afforded 5% degradation, while the addition of Al2O3 induced approximately 54% degradation under the conditions used. The role of hydroxyl radicals in this process was also investigated by examining the effects of a hydroxyl radical scavenger, glutathione. A significant suppression of DNA leakage through the cell membrane was observed in the solutions containing glutathione, and the suppressive effects were dose-dependent. These results indicate that hydroxyl radicals may play a primary role in the degradation of DNA as well as cell lysis. (c) 2006 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE SA, 2006年11月, BIOCHEMICAL ENGINEERING JOURNAL, 32(2) (2), 100 - 105, 英語[査読有り]研究論文（学術雑誌）
- An ultrasonic treatment system, using a TiO2 photocatalyst, was used to disinfect Legionella pneumophila. A kinetic study of the process indicates that TiO2 Significantly improves the disinfection process. The concentrations of viable cells were reduced to 6% of the initial concentrations in the presence of 0.2 g/ml TiO2 after a 30 min of treatment period, while only an 18% reduction was observed in the absence of TiO2. The potency of the disinfection could be enhanced, to some extent, by increasing the amount of TiO2 used. Cell concentrations were decreased by an order of 3 within 30 min of treatment in the presence of 1.0 g/ml TiO2. The disinfection power in the presence of TiO2 versus Al2O3 was also compared and the findings showed that TiO2 induced a higher cell killing. No significant effect of initial cell concentration on the disinfection was found in the range of 10(2)-10(7) CFU/ml after a 30 min of treatment period. The mechanism of cell killing was investigated by examining the effects of OH radical scavengers such as ascorbic acid, histidine and glutathione. The disinfection power was reduced in samples that contained these radical scavengers, thus indicating the importance of OH radicals. (c) 2006 Elsevier Ltd. All rights reserved.PERGAMON-ELSEVIER SCIENCE LTD, 2006年03月, WATER RESEARCH, 40(6) (6), 1137 - 1142, 英語[査読有り]研究論文（学術雑誌）
- Titanium dioxide (TiO_2) is known as photocatalytic material, and it generate reactive oxygen species (ROS) according to be excited by ultraviolet rays (UV) under 380nm. Those ROS generated from TiO_2 could sterilize E coli, and have cytotoxic effect on mammalian cancer cells. Previously, we reported the generation of OH radicals from TiO_2 particles excited by ultrasonic (US) irradiation, not UV. Furthermore, the TiO_2 excited by US exhibited decompose activity on E coli and chemical substances. In this study, we report the cytotoxic effect of US in the presence of TiO_2 particles in cell culture media. To estimate the cytotoxic effect, we measured lactase dehydrogenase (LDH) activity leaked from damaged cell numbers after US irradiation. Immediately after US irradiation in the presence of TiO_2 particles, the LDH activity exhibited high, indicating TiO_2 with US irradiation caused damage on cell membrane stability. Furthermore, we confirmed the cell membrane damage by flowcytometry assay with Annexin V-FITC/Propidium iodide (PI) stain method, Annexin V-negative/PI positive cells were dominant immediately after US irradiation in the presence of TiO_2 particles.日本ソノケミストリー学会, 2006年, ソノケミストリー討論会講演論文集, 15, 1 - 2, 日本語
- Generation of OH radicals by ultrasound irradiation in the presence of TiO_2 was studied. The effects of dissolved argon and xenon applied into the irradiating solutions were studied in the presence of TiO_2. Heat capacity ratio of argon and xenon is higher than that of nitrogen and oxygen. Higher heat capacity ratio of dissolved argon and xenon causes higher local temperature when cavitation bubble collapsed, and it results in the induced pyrolysis of H_2O and generation of OH radicals. Further, the effect of the photocatalysis of TiO_2 may be more intensified by high temperature and sonoluminescence.日本ソノケミストリー学会, 2006年, ソノケミストリー討論会講演論文集, 15, 99 - 100, 日本語
- The formation of H_2O_2 was studied with ultrasound irradiation in the presence of TiO_2 or Al_2O_3 particles. The effect of varying the temperature of cavitation bubbles in aqueous solutions of two rare gases (Ar, Xe or mixture of Xe and Ne (Xe:Ne=3:7)) on the formation of H_2O_2 were estimated. The very high temperatures (several thousand K) of collapsing gas bubbles lead to the thermal dissociation of water molecules into OH radicals, and the reaction of two OH radicals causes the formation of H_2O_2 consequently. After 60 min sonication with 25〜36kHz ultrasound the highest concentration of H_2O_2 (612μM) was observed when Xe was saturated in the solution. While the increased concentration of H_2O_2 after 60 min sonication was lower (135μM) in the solution saturated with Ar or mixture of Xe and Ne. The difference in the increased concentration of H_2O_2 was not significant between Ar and mixture of Xe and Ne. These results suggest that the threshold for the formation of cavitation bubbles seems to be the lowest in the solution saturated with Xe and to be the important factor for the formation of H_2O_2 during the sonication in the presence of TiO_2 or Al_2O_3, respectively.日本ソノケミストリー学会, 2006年, ソノケミストリー討論会講演論文集, 15, 87 - 88, 日本語
- To well characterize disinfection system and to investigate the mechanism of cell killing by TiO_2/US method, Escherichia coli (E. coli) was used as a model and target microorganism. At present, our research is going on developing disinfection system by using ultrasonic reactor in the presence of nonwoven TiO_2 fabric. It was found that the decrease in cell concentration was almost insignificant without ultrasonic irradiation although TiO_2 filter was present, and vice versa. In contrast, applying ultrasonic irradiation with TiO_2 filter reduces the E. coli cell concentration very rapidly which indicated very high disinfection power at this condition or system. However, the effect of new ultrasonic reactors on the disinfection rate, the characterization of affecting parameters of the ultrasonic reactors, as well as possible germicidal mechanism of disinfection remain to be studied. Given an opportunity to conduct these research will uncover above issues and will be benefited our scientific career to develop, in addition to enrich the bio-industrial field as well.日本ソノケミストリー学会, 2006年, ソノケミストリー討論会講演論文集, 15, 103 - 104, 英語
- 公益社団法人 化学工学会, 2006年, 化学工学会 研究発表講演要旨集, 2006, 997 - 997, 日本語
- 公益社団法人 化学工学会, 2006年, 化学工学会 研究発表講演要旨集, 2006, 783 - 783, 日本語
- The kinetics of disinfection of Escherichia coli, in the presence of a TiO2 photocatalyst, using an ultrasonic irradiation system was investigated. TiO2 was found to significantly improve the disinfection process. A 98% reduction in the concentrations of viable cells was obtained in the presence of TiO2 during a 30 min period of irradiation, while only a 13% reduction was observed when an ordinary ultrasonic irradiation system was used. The rate of cell killing was also higher in the presence of TiO2 compared with Al2O3. The rate of disinfection was proportional to the amount of TiO2 in the concentration range examined. Cell concentrations were decreased by an order of 5 within 10 min of irradiation in the presence of 2.0 g/ml TiO2. No significant effect of cell concentration on the cell-killing process was found in the range of 10(3) to 10(7) CFU/ml. The mechanism of cell killing was further investigated by examining the effects of OH radical scavengers, such as histidine and glutathione. The rate of disinfection was decreased in samples containing these radical scavengers, thus indicating that radicals are of major importance in this process. (c) 2005 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE SA, 2005年10月, BIOCHEMICAL ENGINEERING JOURNAL, 25(3) (3), 243 - 248, 英語[査読有り]研究論文（学術雑誌）
- 日本ソノケミストリー学会, 2005年, ソノケミストリー討論会講演論文集, 14, 13 - 14, 英語
- Generation of OH radicals by ultrasonic irradiation in the presence of TiO_2 was studied. OH radicals are oxidizing agents and effective for the degradation of hazardous organic compounds. However, the mechanisms of OH radicals formation have not been elucidated yet. In this work, the effects of cavitation induced by ultrasonic irradiation in water was considered and investigated. As the lifetime of OH radical is very short and can be hardly analyzed directly, then sodium salicylate was used as OH radicals trapping agent. The addition reaction of OH radicals and salicylate results in the formation of dihydroxy benzoic acid(DHBA). The generation of DHBA by ultrasonic irradiation in the presence of TiO_2 as an indicator of OH radicals was investigated in complete darkness. The effects of particles (TiO_2 and A1_2O_3) on the generation of DHBA were examined(Fig.2). While no generation of DHBA was detected in the solutions without particles, higher DHBA concentration was obtained in the presence of TiO_2 than that of Al_2O_3. This enhancement in the formation of OH radicals may be due to the pyrolysis of H_2O and the photocatalysis of TiO_2 with sonoluminessence. Sonoluminessence may be due to cavitation, and the exsitence of particles couse more cavitation whuch may increase sonoluminessence effect. [graph] Also, the effects of dissolved argon applied into the irradiating solutions were studied in the presence of TiO_2 and Al_2O_2 (Fig.5, 6). Higher amount of DHBA was obtained in the solutions in the presence of TiO_2. Thermal capacity of argon is higher than that of nitrogen and oxygen. Higher thermal capacity of dissolved argon causes higher local temperature during cavitation, and it results in the induced pyrolysis of H_2O and generation of OH radicals. Moreover, the effect of the photocatalysis of TiO_2 may be more intensified in the presence of dissolved argon. [graph] [graph] Consequently, the advantage of TiO_2 in the ultrasonic system for the formation of OH radicals was shown in this research.日本ソノケミストリー学会, 2005年, ソノケミストリー討論会講演論文集, 14, 91 - 93, 日本語
- 公益社団法人 化学工学会, 2005年, 化学工学会 研究発表講演要旨集, 2005, 287 - 287, 日本語
- 公益社団法人 化学工学会, 2005年, 化学工学会 研究発表講演要旨集, 2005, 280 - 280, 日本語
- Improvement of ultrasonic disinfection power using TiO2 photocatalystThe disinfection power of an ultrasonic system was enhanced using TiO2 photocatalyst in the irradiating solutions. Cultures of Legionella were used in the irradiation system with and without TiO2. A significant decrease in the concentration of viable cells was observed during irradiation in the presence of TiO2. The rate of cell killing was higher in the presence of TiO2 than it was with Al2O3, and was proportional to the amount of TiO2 used in the irradiating samples. There was no significant effect of cell concentration on the rate of cell killing in the range of 10(3) to 10(7) CFU/ml. Addition of OH radical scavengers such as glutathione, ascorbic acid and histidine to the irradiating solutions reduced the rate of disinfection, thus indicating the primary role of OH radicals in this process.AMER INST PHYSICS, 2005年, 4th International Symposium on Therapeutic Ultrasound, 754, 275 - 277, 英語[査読有り]研究論文（国際会議プロシーディングス）
- 2004年06月, Applied Microbiology and Biotechnology, Vol 64, pp. 823-828, 英語Over-expression system of phospholipase D from actinomycete by Streptomyces lividans[査読有り]研究論文（学術雑誌）
- The addition of short chain polyethylene glycols (PEGs) activated Chromobacterium viscosum lipase in AOT reverse micelles. In this study, it was assumed that when AOT reverse micelles contained PEG molecules, native and activated lipases contributed to the reaction according to their weight fraction in the system. The fractions of individual lipases and their specific activities were estimated by using kinetics parameters determined by previous kinetic models. The maximum specific activity of PEG-activated lipase appeared to be 2.7 times higher than that of native lipase. The predicted weighted-average activity agreed well with experimental data. The results showed that the previous kinetic models could be used to estimate the fraction of lipase activated by PEG molecules and their specific activity. (C) 2004 Society of Chemical Industry.JOHN WILEY & SONS LTD, 2004年03月, JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 79(3) (3), 273 - 276, 英語[査読有り]研究論文（学術雑誌）
- A phospholipase D (PLD) expression system was constructed from Streptoverticillium cinnamoneum using Escherichia coli as a host. From a sequence alignment analysis of PLD superfamily, a conserved sequence, a GG/S motif, was found, next to the putative active center in the PLD superfamily. Based on the hypothesis that a serine residue in GG/S motif is involved in the catalytic function, a serine residue substituted mutant library (sixteen PLD mutants) was constructed. An analysis of the activities of these mutants showed that two serine residue substituted mutants (G215S and G216S) in the GG motif exhibited high conversion activity. However, the substrate specificity of these mutants differed depending on the donor molecules. When a serine amino acid was used as a donor substrate, G216S showed a high transphosphatidylation activity. For other donors, G215S showed a higher catalytic activity than G216S. These results support the hypothesis that the serine residue in the GG/S motif controls substrate specificity and enhances the turnover of catalytic function.公益社団法人 化学工学会, 2004年, アジア・太平洋化学工学会議発表論文要旨集, 2004, 810 - 810, 英語
- A disinfecting method has been considered by ultrasonic irradiation of water in the presence of TiO2. Titanium dioxide in the rutile-granule form was used as a catalyst in order to generate hydroxyl radicals for cell killing. Escherichia coli and Legionella pneumophila were used for kinetically investigation of cell destruction process. Significant decrease in the concentration of viable cells was observed during irradiation in the presence of TiO2. About 98% reduction in the concentration of viable cells could be obtained in the presence of 1.0 g/ml TiO2 during 30 min of irradiation, while only 13% reduction was observed in the ordinary ultrasonic irradiation system. The rate of cell killing was higher in the presence of TiO2 comparing to Al2O3. The rate of disinfection was proportional to the amount of TiO2 used in the irradiated samples. There was no significant effect of cell concentration on the cell-killing rate in the range of 103 to 107 CFU/ml. Mechanism of cell killing was further investigated by examining the effect of hydroxyl radical (*OH) scavengers. Ascorbic acid, histidine and glutathione were used in the irradiating samples as radical scavengers. The rate of disinfection was decreased in the samples containing these radical scavengers. Also, a continuous irradiating system was devised and applied for high throughput water disinfection process. Contaminated water containing E. coli was disinfected successfully within two hours of irradiation in the continuous system.公益社団法人 化学工学会, 2004年, アジア・太平洋化学工学会議発表論文要旨集, 2004, 610 - 610, 英語
- Introduction Titanium dioxide in the rutile-pellet form was used as a catalyst in the ultrasonic irradiation system to generate hydroxyl radicals in order to disinfect the water. Cultivated culture of Legionella was used for the kinetically investigation of cell destruction and its mechanism. The formation of active species such as hydroxyl radical, hydrogen peroxide, superoxide and etc can be promoted during ultrasonic irradiation in the presence of TiO_2. It may be due to the concentrated energy of the exploded bubbles on the surfaces of titanium granules caused during acoustic cavitations. These reactive oxygen species (ROS) may disrupt or damage various cell or viral functions and structures. Improvement of this novel disinfection method and studying the mechanism of this system were the purposes of this investigation. Materials and methods -Preparation of Cultures Legionella pneumophila(GIFU 9888) was used in this research as the model microorganisms. Legionella were incubated on BCYE plate and transferred to BYE medium. The inoculated culture was incubated in a shaker incubator at 110 rpm for 48 hrs at 37℃. Then, the culture broth was diluted to a suitable concentration. Ten ml of the diluted broth was transferred to the bottles containing TiO_2 pellets at different concentrations. -Ultrasonic irradiation Bottles were irradiated at 36 KHz, 300 W and samples were driven at different time courses. The temperature was kept constant at 20℃ during irradiation. The irradiated samples were diluted to suitable concentrations and plated on BCYE plates. The plates were incubated for 72 hrs at 37℃. The number of colonies on the plates was counted after incubation. Results and Discussion Ultrasonic irradiation of diluted culture for 15 minutes showed 92% reduction in the number of viable cell concentration at the presence of TiO_2. The rate of disinfection was compared in the presence of TiO_2 and Al_2O_3. TiO_2 induced higher rate of cell killing compared with Al_2O_3. Also, investigating the effect of TiO_2 concentration indicated that the rate of disinfection was proportional to the concentration of TiO_2. The mechanism of cell killing was further investigated by examining the effects of OH radical scavengers such as ascorbic acid and glutathione. Lower rates of disinfection were observed in the samples containing these radical scavengers, thus indicating the primary role of OH radicals in this process.日本ソノケミストリー学会, 2004年, ソノケミストリー討論会講演論文集, 13, 38 - 40, 日本語
- Irradiation of TiO_2 particles under ultraviolet light has been known to be a source of OH radicals in the disinfection or oxidizing systems. However, a new system was devised in this study for production of OH radicals by replacing UV with ultrasonic system (US). Formation of OH radicals was investigated in a TiO_2/US system to study the mechanism of formation of OH radicals as well as enhancement of cavitation effect in the ultrasonic system. Formation of OH radicals in the irradiating solution was studied by the reaction of OH radicals with salicylate. 2,3-dihydroxybenzoicacid (2,3-DHBA) and 2,5-dihydroxybenzoicacid (2,5-DHBA) can be formed during this reaction and they can be measured by HPLC with an electro chemical detector (HPLC-ECD; Eicom). Formation of DHBAs could be detected during the irradiation of salicylate solution under ultrasonic system and in the presence of TiO_2. It was seen that the concentration of DHBAs was increased within 20 minutes irradiation and then decreased in spite of high concentration of salicylate. It was likely due to the oxidation and degradation of DHBA in the ultrasonic system. Therefore, for investigating the oxidation of DHBA in the presence of salicylate, more salicylate was added after 20 minutes reaction to the irradiating mixture consecutively. The concentration of DHBA was increased for three times by the addition of salicylate concentration in the solution. Enhancement of cavitation energy was also investigated by the addition of various gases (Ar, O2, N2) into irradiation solution. The ratio of the specific heats at constant pressure and constant volume (Cp/Cv) of the gas and vapor inside the cavity is known to be one of the most crucial parameters that determine the final temperature inside cavitation bubbles. Monoatomic gases, such as argon has a high value of Cp/Cv, while diatomic gases, such as N_2, O_2 have a lower values. Therefore, addition of argon to the irradiating solution induced higher concentration of OH radicals due to higher cavitation energy. Our results indicated that the addition of O_2 and N_2 gases at the same conditions resulted lower oxidation power. Finally, the competitive oxidation of salicylate and DHBAs in the irradiating solutions was more investigated in this research to develop a more descriptive mechanism of generation of hydroxyl radicals.日本ソノケミストリー学会, 2004年, ソノケミストリー討論会講演論文集, 13, 94 - 96, 日本語
- 2004年, Applied and Microbiogy and Biotechnology, 64,823-828, 英語Over-expression system for secretory phospholipase D by Streptomyces livedanse[査読有り]研究論文（学術雑誌）
- TOL plasmid pWW0 of Pseudomonas putida mt-2 encodes a series of enzymes for the degradation of benzene derivatives. In the presence of benzene derivatives, the complex formed from the regulating protein XylR and Ps promoter controls the expression of these enzymes in P. putida. For detecting of benzene derivatives, the plasmid called as pTS301-GFP has been constructed by introducing a gene encoding green fluorescent protein (GFP) into the downstream of the Ps promoter gene. GFP functioned as a reporter protein is transcribed by activation of the XylR complex. In the present study, the effects of the composition of culture medium and ATP addition on fluorescence of transformant were investigated. The optimal culture medium was found to NZM, and presence of casamino acid decreases the sensitivity for detection of chemicals. Furthermore, it was observed that the addition of 200 muM ATP was effective for detecting of fluorescence of transformants exposed to the low concentration (0.05 mM) of benzene derivatives for 4 h. Applying these results for the detection of the benzene derivatives, it will be expected that transformant with pTS301-GFP can be useful for environmental water evaluation. (C) 2003 Elsevier Science B.V. All rights reserved.ELSEVIER SCIENCE SA, 2003年12月, BIOCHEMICAL ENGINEERING JOURNAL, 16(3) (3), 273 - 278, 英語[査読有り]研究論文（学術雑誌）
- This review is focusing on an industrially important enzyme, phospholipase D (PLD), exhibiting both transphosphatidylation and hydrolytic activities for various phospholipids. The transphosphatidylation activity of PLD is particularly useful for converting phosphatidylcholine (PC) into other phospholipids. During the last decade, the genes coding for PLD have been identified from various species including mammals, plants, yeast, and bacteria. However, detailed basic and applied enzymological studies on PLD have been hampered by the low productivity in these organisms. Efficient production of a recombinant PLD has also been unsuccessful so far. We recently isolated and characterized the PLD gene from Streptoverticillium cinnamoneum, producing a secretory PLD. Furthermore, we constructed an overexpression system for the secretory enzyme in an active and soluble form using Streptomyces lividans as a host for transformation of the PLD gene. The Stv. cinnamoneum PLD was proven to be useful for the continuous and efficient production of phosphatidylethanolamine (PE) from phosphatidylcholine. Thus, the secretory PLD is a promising catalyst for synthesizing new phospholipids possessing various polar head groups that show versatile physiological functions and may be utilized in food and pharmaceutical industries. (C) 2003 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, 2003年09月, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 23(2-6) (2-6), 107 - 115, 英語[査読有り]研究論文（学術雑誌）
- The TOL plasmid of Pseudomonas putida mt-2 encodes a series of enzymes for the degradation of benzene derivatives. The expression of the enzymes is controlled by the regulating protein XylR, of which the promoter, known as Ps, is activated in the presence of benzene derivatives. Sensitive and convenient sensing of benzene derivatives has been developed by fusing a gene encoding a green fluorescent protein (GFP) into the downstream of the Ps promoter. In the plasmid pTS301-GFP, GFP as a reporter protein is transcribed by controlling the promoter's activation by Xy1R and benzene derivatives. In the present study, a recombinant Escherichia coli transformed with this plasmid was applied to the environmental sensing of benzene derivatives in water. The expression of GFP was confirmed in water containing 0.1 mM m-xylene and fluorescence intensity was quantified by microplate reader. The optimal temperature for GFP expression was 27 degreesC and the lowest detectable concentration of xylene derivatives 0.1 mM. Benzene derivatives having CH3- or Cl- or both in the side chain of the benzene ring showed particularly high fluorescence intensity. To conclude, transformants harboring pTS301-GFP constitute an adaptable system for easy sensing of small amounts of benzene derivative in water. (C) 2003 Elsevier Science B.V. All rights reserved.ELSEVIER SCIENCE SA, 2003年09月, BIOCHEMICAL ENGINEERING JOURNAL, 15(3) (3), 193 - 197, 英語[査読有り]研究論文（学術雑誌）
- Improvement in enzyme activity and stability by addition of low molecular weight polyethylene glycol to sodium bis(2-ethyl-L-hexyl)sulfosuccinate/isooctane reverse micellar systemThe activity and stability of Chromobacterium viscosum lipase (glycerolester hydrolase, EC 3.1.1.3)-catalyzed olive oil hydrolysis in sodium bis (2-ethyl-1-hexyl)sulfosuccinate (AOT)/isooctane reverse micelles is increased appreciably when low molecular weight polyethylene glycol (PEG 400) is added to the reverse micelles. To understand the effect of PEG 400 on the phase behavior of the reverse micellar system, the phase diagram of AOT/ PEG 400/water/isooctane system was studied. The influences of relevant parameters on the catalytic activity in AOT/PEG 400 reverse micelles were investigated and compared with the results in the simple AOT reverse micelles. In the presence of PEG 400, the linear decreasing trend of the lipase activity with AOT concentration, which is observed in the simple AOT reverse micelles, disappeared. Enzyme entrapped in AOT/PEG reverse micelles was very stable, retaining >75% of its initial activity after 60 d, whereas the half-life in simple AOT reverse micelles was 38 d. The kinetics parameter maximum velocity (V-max) exhibiting the temperature dependence and the activation energy obtained by Arrhenius plot was suppressed significantly by the addition of PEG 400.HUMANA PRESS INC, 2003年08月, APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 110(2) (2), 101 - 112, 英語[査読有り]研究論文（学術雑誌）
- 日本ソノケミストリー学会, 2003年, ソノケミストリー討論会講演論文集, 12, 30 - 32, 日本語
- Structural investigation of water trapped in AOT/isooctane reverse micelles containing PEG by Fourier transform infrared spectroscopyThe structure of water entrapped in AOT/PEG 400 reverse micelles was investigated and compared with that of simple AOT reverse micelles by using Fourier transform infrared spectroscopy (FT-IR) to measure the stretching vibrations for O-H, carbonyl (C=O) and SO3- (S=O) bonds. The water in a reverse micelle consists of trapped water, bound water and free water. The fraction of free water in the water pool of reverse micelles containing PEG increases with the molar ratio of AOT to H2O, W-o more steeply than that of simple reverse micelles, and the structure of bound water is disrupted by the addition of PEG. The shift of the peak of the S = O bond and analysis of the C = O bond spectra indicates that the interaction of AOT molecules with water is decreased by hydrated PEG molecules at W-o > 6, which coincides with the results of O-H spectra.SOC CHEMICAL ENG JAPAN, 2003年01月, KAGAKU KOGAKU RONBUNSHU, 29(1) (1), 124 - 130, 日本語[査読有り]研究論文（学術雑誌）
- Improvement of transphosphatidylation reaction model of phospholipase D from Streptoverticillium cinnamoneumA new reaction model for transphosphatidylation and hydrolysis reactions by PLD has been constructed and simulated under various experimental conditions. The reaction model is based on the Ping-Pong mechanism and composed of PLD-phosphatidyl complex intermediates. In order to accommodate the inhibition of the initial reaction rate at high nucleophile concentration, a phosphatidyl-enzyme complex associating with two nucleophile molecules [P-PLD-X-2] is introduced into the transphosphatidylation scheme of the proposed reaction model. In both cases (phosphatidylethanolamine (PE) production, and phosphatidylglycerol (PG) production from phosphatidylcholine (PC)), the simulation results agreed well with the experimental data. While the PE production rate at the initial state was inhibited at high concentrations of the nucleophile (ethanolamine), the PG production rate did not show inhibition. Combination of the PE and PG production schemes is expected to make multi-phospholipid production from PC possible, thus, making this model of potential use for phospholipid synthesis in engineering applications. (C) 2002 Elsevier Science B.V All rights reserved.ELSEVIER SCIENCE SA, 2002年03月, BIOCHEMICAL ENGINEERING JOURNAL, 10(2) (2), 115 - 121, 英語[査読有り]研究論文（学術雑誌）
- Heterogeneous photocatalysis over TiO_2 or ultrasonic irradiation have been reported to be a promising application for decomposition of the hazardous chemical substances. We observed the generation of ・OH in the aqueous solution during the irradiation of ultrasound on TiO_2. The purpose of this study was to verify the generation of ・OH by indicating (1) decomposition of methylene blue, (2) nucleophilic addition of ・OH to salicylic acid, which produce dihydroxybenzoic acid. The decomposition of bisphenol A (BPA) and pentachlorophenol (PCP) by the irradiation of ultrasound in presence of TiO_2 were further studied. The results are summarized as follows. Decomposition of methylene blue Experimentally it was observed that although the Al_2O_3 had no effect on the decomposition of methylene blue by ultrasonic irradiation, it was decomposed slightly by the presence of TiO_2. In addition H_2O_2 accelerated the decomposition of methylene blue for the TiO_2 containing system. But the addition of H_2O_2 had no effect on the methylene blue decomposition when the system containing Al_2O_3 only. The effect of radical scavengers on the decomposition of methylene blue was also investigated for the system with TiO_2. It was found that the scavengers such as methanol, DMSO, mannitol reduced the decomposition. Among them DMSO was the most significant. Effect of TiO_2 on the nucleophilic addition of ・OH to salicylic acid Ultrasonification of 10 μM salicylic acid (2 ml) was carried out in absence and presence of 0.4 g TiO_2 for exactly 10 minutes, and produced dihydroxybenzoic acid (DHBA) was measured by HPLC/ECD. In presence of TiO_2, significant amount of DHBA (34 pg/20μl) was produced, but almost no DHBA (2.2 pg/20μl) was found to produce without TiO_2. The effect of scavengers on the generation of DHBA was the same as it was observed in the case of methylene blue decomposition. This result inferred that the ultrasonic irradiation generated the ・OH, which is responsible for the hydroxylation of salicylic acid. Decomposition of BPA and PCP Ultrasonification of BPA and PCP, a representative endocrine disruptors, were carried out in the presence of TiO_2 (0.4 g) and the change in the concentration of BPA and PCP were measured by HPLC/ECD. The initial concentration of BPA (1 ppm) was gradually decreased with the irradiation time and was totally decomposed by 15 minute irradiation of ultrasound. The similar results were observed for PCP. It was concluded that the irradiation of ultrasound on TiO_2 in aqueous solution significantly generate hydroxyl radicals which is responsible for the decomposition of the endocrine disruptors such as BPA and PCP.日本ソノケミストリー学会, 2002年, ソノケミストリー討論会講演論文集, 11, 91 - 93, 日本語
- To quantify individual behavioral responses to toxic chemicals, the swimming motion of individual Daphnia magna was continuously monitored using a motion analysis system. The fractal dimension was introduced to compare the straightness or complexity of the swimming trajectory before and after exposure to toxic chemicals. Analysis indicated that the swimming trajectory of individual Daphnia has a fractal structure. The basal fractal dimension in the control medium was 1.35±0.01 (n = 50 Daphnia). Exposure to CuSO4, (10 μg/L), organophosphorus (Dichlorvos 10 μg/L), and carbamate (Propoxur 500 μg/L) pesticide caused a significant increase in the fractal dimension with a latency of 60 min, reaching a maximal level of 2.26±0.34, 2.43±0.19, and 2.51±0.21, respectively, after a 120-min exposure. The magnitude of the change in the fractal dimension was related to the toxic chemical concentration and the exposure time. Threshold concentrations determined at 60 min of exposure were 10 μg/L for CuSO4, 5 μg/L for Dichlorvos, and 500 μg/L for Propoxur. The toxicity index (EC50) values after 120 min of exposure were 6.31 μg/L, 7.64 μg/L, and 466 μg/L for CuSO4, Dichlorvos, and Propoxur, respectively. Thus, the fractal dimension seems useful for analyzing and comparing complex trails, such as swimming trajectories, which could be used as the endpoint for an acute bioassay. © 2002 Wiley Periodicals, Inc.2002年, Environmental Toxicology, 17(5) (5), 441 - 448, 英語[査読有り]研究論文（学術雑誌）
- Identification of novel membrane-bound phospholipase D from Streptoverticillium cinnamoneum, possessing only hydrolytic activityA membrane-bound phospholipase D (PLD) has been identified and isolated in a soluble form from an actinomycete, Streptoverticillium cinnamoneum. The enzyme has a monomeric structure with a molecular size of about 37 kDa, bring the smallest among the enzymes so far reported. The enzyme catalyzes the hydrolysis of phosphatidylethanolamine and phosphatidylserine as preferred substrates, but not the transphosphatidylation reaction of their phospholipid groups to ethanol. Together with the absence of immunochemical cross-reactivity, these enzymatic properties demonstrate that the membrane-bound enzyme is distinct from the extracellular enzyme recently characterized and cloned from the same bacterial strain [C. Ogino ct al., J. Biochem. 125 (1999) 263-269] and is therefore regarded as a novel prokaryotic PLD. (C) 2001 Elsevier Science B.V. All rights reserved.ELSEVIER SCIENCE BV, 2001年01月, BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1530(1) (1), 23 - 31, 英語[査読有り]研究論文（学術雑誌）
- The 5-HT1A receptor agonist, 8-OH-DPAT, attenuates stress-induced anorexia in conjunction with the suppression of hypothalamic serotonin release in ratsThe effect of the selective 5-HT1A receptor agonist 8-hydroxy-2-(di-n-propylamino) tetralin (8-OH-DPAT) on stress-induced anorexia and serotonin (5-HT) release in the rat hypothalamus was studied with brain microdialysis. Subcutaneous injection of 8-OH-DPAT (1 mg/kg) significantly attenuated the immobilization-induced anorexia for 3 h, but had no effect during the following 9 h. Injection of 8-OH-DPAT itself had no effect on basal release of 5-HT, while it significantly blocked the immobilization-induced 5-HT release in the lateral hypothalamus. The results suggest that 8-OH-DPAT attenuated the stress-induced anorexia through the activation of 5-HT1A autoreceptors in dorsal raphe nucleus. (C) 2000 Elsevier Science B.V. All rights reserved.ELSEVIER SCIENCE BV, 2000年12月, BRAIN RESEARCH, 887(1) (1), 178 - 182, 英語[査読有り]研究論文（学術雑誌）
- Purification, characterization, and sequence determination of phospholipase D secreted by Streptoverticillium cinnamoneumPhospholipase D (PLD), secreted into the culture medium of an actinomycete, Streptoverticillium cinnamoneum, has been purified to homogeneity and characterized. The Stv. cinnamoneum PLD efficiently catalyzes both the hydrolysis and transphosphatidylation of various phospholipids, including phosphatidylethanolamine (PE), phosphatidylcholine (PC), and phosphatidylserine (PS), However, the substrate specificity differs between the two reactions; PE serves as the most preferred substrate for the hydrolysis, but PC and PS are better substrates than PE for the transphosphatidylation. In addition, the transphosphatidylation but not the hydrolysis of PE and PC is markedly activated on the addition of metal ions, especially Al3+. Nucleotide and amino acid sequence determination of the Sty. cinnamoneum PLD revealed the presence of common structural motifs identified in all PLD sequences from various species.OXFORD UNIV PRESS, 1999年02月, JOURNAL OF BIOCHEMISTRY, 125(2) (2), 263 - 269, 英語[査読有り]研究論文（学術雑誌）
- For activation of kidney membrane phospholipase D (PLD), cytosol isabsolutely needed in addition to GTP-γ-S. The active component of cytosol consists of three protein factors: ADP-ribosylation factor, RhoA, and a soluble 36-kDa protein. Any combination of these two factors synergistically activates PLD to some extent, but the presence of the three factors causes full activation. The 36-kDa protein is stable at 60°C but inactivated at 80°C for 10 min. Tissue distribution of the 36-kDa protein roughly coincides with that of PLD, suggesting physiological relevance of the protein in the regulation of PLD.Elsevier B.V., 1996年06月, FEBS Letters, 387(2-3) (2-3), 141 - 144, 英語[査読有り]研究論文（学術雑誌）
- Bovine kidney phospholipase D (PLD) was assayed by measuring the formation of phosphatidylethanol from added radioactive phosphatidylcholine (PtdCho) in the presence of ethanol, guanosine 5'-[γ-thio]triphosphate, ammonium sulfate, and cytosol factor that contained small GTP-binding regulatory proteins. The PLD enzyme associated with particulate fractions was solubilized by deoxycholate and partially purified by chromatography on a heparin-Sepharose column. This PLD preferentially used PtdCho as substrate. After purification, the enzyme per se showed little or practically no activity but required an additional factor for the enzymatic reaction. This factor was extracted with chloroform/methanol directly from particulate fractions of various tissues, including kidney, liver, and brain, and identified as phosphatidylethanolamine (PtdEtn), although this phospholipid did not serve as a good substrate. Plasmalogen-rich PtdEtn, dioleoyl-PtdEtn, and L-α-palmitoyl-β-linoleoyl PtdEtn were effective, but dipalmitoyl- PtdEtn was inert. Sphingomyelin was 30% as active as PtdEtn. The results suggest that mammalian PLD reacts nearly selectively with PtdCho in the form of mixed micelles or membranes with other phospholipids, especially PtdEtn.1996年04月, Proceedings of the National Academy of Sciences of the United States of America, 93(9) (9), 4300 - 4304, 英語[査読有り]研究論文（学術雑誌）
- Phospholipase D (PLD) associated with the rat kidney membrane was activated by guanine 5'-[γ-thio]triphosphate and a cytosol fraction that contained ADP-ribosylation factor. When assayed by measuring the phosphatidyl transfer reaction to ethanol with exogenously added radioactive phosphatidylcholine as substrate, the PLD required a high concentration (1.6 M) of ammonium sulfate to exhibit high enzymatic activity. Other salts examined were far less effective or practically inactive, and this dramatic action of ammonium sulfate is not simply due to such high ionic strength. Addition of ATP but not of nonhydrolyzable ATP analogue adenosine 5'-[β,γ- imido]diphosphate further enhanced the PLD activation ≃2- to 3-fold. This enhancement by ATP needed cytosol, implying a role of protein phosphorylation. A survey of PLD activity in rat tissues revealed that, unlike in previous observations reported thus far, PLD was most abundant in membrane fractions of kidney, spleen, and liver in this order, and the enzymatic activity in brain and lung was low.1995年12月, Proceedings of the National Academy of Sciences of the United States of America, 92(26) (26), 12319 - 12322, 英語[査読有り]研究論文（学術雑誌）
- As the third most plentiful biopolymer after other lignocellulosic derivates such as cellulose and hemicellulose, lignin carries abundant potential as a substitute for petroleum-based products. However, the efficient, practical, value-added product valorization of lignin remains quite challenging. Although several studies have reviewed the valorization of lignin by microorganisms, this present review covers recent studies on the valorization of lignin by employing yeast to obtain products such as single-cell oils (SCOs), enzymes, and other chemical compounds. The use of yeasts has been found to be suitable for the biological conversion of lignin and might provide new insights for future research to develop a yeast strain for lignin to produce other valuable chemical compounds.MDPI, 2022年10月, PROCESSES, 10(10) (10), 2004 - 2004, 英語[査読有り]
- 2022年04月, 化学工学 [特集]「生物機能を利用したモノづくり」に貢献するプロセス強化, 86(4) (4), 151 - 152, 日本語バイオエコノミーの展望記事・総説・解説・論説等（学術雑誌）
- 2022年, 日本生物工学会大会講演要旨集, 74th様々な酵母種間に見られる代謝戦略の比較解析
- 2021年, 化学工学会年会研究発表講演要旨集(CD-ROM), 86thパーム油産業廃棄物POMEを原料とする微生物による酵素・化学品への変換
- 2021年, 日本生物工学会大会講演要旨集, 73rd酵母における有機酸資化経路の確立とバイオリファイナリー展開
- 2021年, 日本生物工学会大会講演要旨集, 73rd油脂酵母株Lipomyces starkeyiによるポリヒドロキシアルカン酸生産
- 2021年, 日本生物工学会大会講演要旨集, 73rd油脂酵母の増殖期と油脂生産期の13C代謝フラックス解析
- 2021年, 質量分析総合討論会講演要旨集, 69th13C代謝フラックス解析の油脂酵母Yarrowia lipolyticaへの適用
- 2020年, 日本農芸化学会中部支部例会講演要旨集(Web), 187th好冷性海洋細菌が産生するβ-1,3-キシラナーゼの機能解析
- 2019年, 日本生物工学会大会講演要旨集, 71st油脂酵母Lipomyces starkeyiの遺伝子的改変による長鎖長脂肪酸の生産
- 2019年, 日本生物工学会大会講演要旨集, 71st好冷性海洋細菌Psychroflexus torquis由来β-1,3-キシラナーゼの酵素化学的諸性質
- 2018年08月07日, 日本生物工学会大会講演要旨集, 70th, 191, 日本語機械学習を用いた酵素のEC number予測
- 日本DDS学会, 2018年05月, 日本DDS学会学術集会プログラム予稿集, 34回, 204 - 204, 日本語過酸化チタンナノ粒子の併用による放射線増感治療法の開発
- 2018年03月05日, 日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 68th, ROMBUNNO.Z14‐02‐0930, 日本語バイオマスのイオン液体前処理と酵素糖化により得られる残渣リグニンのアルカリ酸化分解特性
- 2017年, 日本作物学会講演会要旨集, 243rdデンプン合成制御因子CRCTが稲わらを用いたバイオエタノール生産に及ぼす効果
- 2016年10月14日, リグニン討論会講演集, 61st, 180‐181, 日本語疎水性アルコールを用いた植物バイオマスの効率的な成分分離法の開発
- 2016年08月25日, 日本生物工学会大会講演要旨集, 68th, 316, 日本語凝集性酵母における新規な阻害剤耐性機構の解明
- 2016年03月13日, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N316, 英語Yeast breeding for fuels and chemicals production from Indonesia Culture Collection
- 2016年03月13日, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N321, 英語Economic Value of Current Main Use of Oil Palm Empty Fruit Bunches (EFB) and Its Potential Use for Biofuel
- 2016年03月13日, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N302, 英語Biorefinery research in Kobe and Indonesia
- 2016年03月13日, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N313, 英語Biorefinery as strategic approach for supporting of utilization of tropical lignocellulosic biomas
- 2016年03月05日, 日本農芸化学会大会講演要旨集(Web), 2016, 4A022 (WEB ONLY), 日本語マンナンバイオマスからのエタノール生産:β‐マンナナーゼとβ‐マンノシダーゼを細胞表層に提示した出芽酵母の開発
- 2016年03月05日, 日本農芸化学会大会講演要旨集(Web), 2016, 3A030 (WEB ONLY), 日本語アルコールの添加が希硫酸前処理の効率に与える影響の解析
- 2016年, 日本農芸化学会大会講演要旨集(Web), 2016紙パルプの同時糖化発酵によるカフェ酸の微生物生産
- AMER ASSOC CANCER RESEARCH, 2015年08月, CANCER RESEARCH, 75, 英語研究発表ペーパー・要旨（国際会議）
- 2015年03月09日, 日本植物生理学会年会要旨集, 56th, 284, 日本語2次元NMRを用いた希硫酸前処理後固体画分の組成解析
- 2015年03月05日, 日本農芸化学会大会講演要旨集(Web), 2015, 3B33A01 (WEB ONLY), 日本語実バイオマスを微生物変換するための新しい酵母プラットフォームの探索
- 2015年, 育種学研究, 17バイオリファイナリー利用に向けた稲わらの多様性
- 日本生物工学会, 2015年, 日本生物工学会大会講演要旨集, 67, 95 - 95, 日本語1P-025 代謝改変による赤色酵母を用いたアスタキサンチン生産性の向上(遺伝子工学,一般講演)
- 日本生物工学会, 2015年, 日本生物工学会大会講演要旨集, 67, 320 - 320, 日本語3P-198 Streptoverticillium Cinnamoneum由来Phospholipase Dプロモーター遺伝子発現機構の調査(バイオプロセス,一般講演)
- 日本生物工学会, 2015年, 日本生物工学会大会講演要旨集, 67, 218 - 218, 日本語2P-173 セルラーゼへの阻害の少ないコリン酢酸を前処理に用いたバガスのin situ 酵素糖化(バイオマス,資源,エネルギー工学,一般講演)
- 日本生物工学会, 2015年, 日本生物工学会大会講演要旨集, 67, 217 - 217, 日本語2P-170 酸性イオン液体により前処理・分画された木質系バイオマスからのフラン系モノマーの生産(バイオマス,資源,エネルギー工学,一般講演)
- 日本生物工学会, 2015年, 日本生物工学会大会講演要旨集, 67, 217 - 217, 日本語2P-171 最少量のイオン液体で前処理したバイオマスからの高濃度糖化・発酵生産(バイオマス,資源,エネルギー工学,一般講演)
- 日本生物工学会, 2015年, 日本生物工学会大会講演要旨集, 67, 217 - 217, 日本語2P-172 イオン液体前処理と酵素糖化により得られる残渣リグニンを原料としたリグノモノマーの生産(バイオマス,資源,エネルギー工学,一般講演)
- 日本生物工学会, 2015年, 日本生物工学会大会講演要旨集, 67, 199 - 199, 日本語2P-097 L-乳酸生産株Aspergillus oryzae の代謝改変によるL-乳酸生産能の改変(発酵生理学,発酵工学,一般講演)
- (公社)日本医学放射線学会, 2014年02月, Japanese Journal of Radiology, 32(Suppl.) (Suppl.), 37 - 37, 日本語過酸化チタンナノ粒子による放射線増感の検討
- 日本生物工学会, 2014年, 日本生物工学会大会講演要旨集, 66, 206 - 206, 日本語3P-045 バイオマス糖化液由来成分が大腸菌のフェニル乳酸発酵に与える影響(オミクス解析,一般講演)
- 日本生物工学会, 2014年, 日本生物工学会大会講演要旨集, 66, 251 - 251, 日本語3P-226 pH 応答性ペプチドGALAを表層提示したバイオナノカプセルのエンドソーム脱出(生物化学工学,一般講演)
- 日本生物工学会, 2014年, 日本生物工学会大会講演要旨集, 66, 72 - 72, 日本語1P-219 稲わら前処理液の膜プロセスによるエタノール発酵高効率化(バイオマス,資源,エネルギー工学,一般講演)
- 日本生物工学会, 2014年, 日本生物工学会大会講演要旨集, 66, 28 - 28, 日本語1P-044 麹菌を用いたデンプンからのD- 乳酸の同時糖化発酵(遺伝子工学,一般講演)
- 日本生物工学会, 2014年, 日本生物工学会大会講演要旨集, 66, 45 - 45, 日本語1P-112 放線菌を用いた糖を原料とするパラアミノ安息香酸の生産(発酵生理学,発酵工学,一般講演)
- 日本生物工学会, 2014年, 生物工学会誌 : seibutsu-kogaku kaishi, 92(2) (2), 71 - 71, 日本語合成代謝経路と細胞表層工学を利用した組換え大腸菌によるセロビオースからのイソプロパノール生産(生物工学論文賞)
- (一社)日本消化器外科学会, 2013年07月, 日本消化器外科学会総会, 68回, O - 82, 日本語超音波触媒法による胆管ステント再閉塞予防の検討
- 小峰工業出版, 2013年02月, 化學工業, 64(2) (2), 136 - 139, 日本語イオン液体前処理バイオマスを用いたエタノール同時糖化発酵 (特集 イオン液体応用技術の新展開)
- 日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 47 - 47, 日本語1P-118 放線菌による芳香族化合物ホモゲンチジン酸の生産(一般講演(発酵生理学,発酵工学))
- 日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 44 - 44, 日本語1P-105 同時糖化発酵によるセルロースからのフェニル乳酸生産(一般講演(発酵生理学,発酵工学))
- 日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 47 - 47, 日本語1P-117 コリネ型細菌を用いたプロトカテク酸の生産(一般講演(発酵生理学,発酵工学))
- 日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 63 - 63, 日本語1P-183 低細胞毒性イオン液体により前処理した木質系バイオマスからの同時糖化発酵(一般講演(バイオマス,資源,エネルギー工学))
- 日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 72 - 72, 日本語1P-218 バイオマス分解性向上を目的とする酸導入イオン液体を用いた新規前処理法開発(一般講演(バイオマス,資源,エネルギー工学))
- 日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 141 - 141, 日本語2P-149 低コストイオン液体によるエタノール製造プロセスの提案(バイオマス,資源,エネルギー工学,一般講演)
- 日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 141 - 141, 日本語2P-148 稲わら希硫酸処理液のナノフィルトレーションによる糖濃縮(バイオマス,資源,エネルギー工学,一般講演)
- 日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 231 - 231, 日本語3P-174 上皮成長因子受容体を特異的に認識するAffibody提示バイオナノカプセルの開発(生物化学工学,一般講演)
- 2012年11月05日, 化学工学 = Chemical engineering, 76(11) (11), 722 - 722, 日本語神戸大学大学院工学研究科応用化学専攻 生物化学工学研究室
- ELSEVIER SCIENCE INC, 2012年11月, INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 84(3) (3), S700 - S700, 英語Novel Radiosensitization Through Reactive Oxygen Species Generation Using Titanium Peroxide Nanoparticle Compounds Against Pancreas Cancer研究発表ペーパー・要旨（国際会議）
- ELSEVIER SCIENCE BV, 2012年09月, NEW BIOTECHNOLOGY, 29, S50 - S50, 英語研究発表ペーパー・要旨（国際会議）
- (一社)日本消化器外科学会, 2012年07月, 日本消化器外科学会総会, 67回, 1 - 1, 日本語ナイチノールステント・超音波触媒法による再閉塞予防の検討
- (一社)日本外科学会, 2012年03月, 日本外科学会雑誌, 113(臨増2) (臨増2), 126 - 126, 日本語外科領域における先端技術・治療の開発 酸化チタン・超音波触媒法によるラジカル生成を利用した肝胆膵悪性腫瘍に対する医工融合型治療法の開発
- (一社)日本外科学会, 2012年03月, 日本外科学会雑誌, 113(臨増2) (臨増2), 389 - 389, 日本語
- 日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 248 - 248, 日本語4Ip22 イオン液体前処理とキシロース資化性酵母株を用いたバイオマス糖化発酵プロセスの効率化(バイオマス,資源,エネルギー工学,一般講演)
- 日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 239 - 239, 日本語4Ia02 イオン液体前処理による木質系バイオマスからのエタノール生産(バイオマス,資源,エネルギー工学,一般講演)
- 日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 230 - 230, 日本語4Ha05 放線菌バイオマス分解酵素ライブラリの構築及び新規高分泌シグナルの探索(生物化学工学,一般講演)
- 日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 186 - 186, 日本語4Ba09 Cell SELEX法により選抜したヒト肝臓由来がん細胞に対するDNAアプタマーの評価(セル&ティッシュエンジニアリング/生体医用工学,人工臓器,一般講演)
- 日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 208 - 208, 日本語4Dp13 エンドグルカナーゼ分泌生産型放線菌によるセルロースからの安息香酸生産(発酵生理学,発酵工学,一般講演)
- 日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 205 - 205, 日本語4Dp02 コリネ型細菌を用いた糖を原料とするカダベリンの生産(発酵生理学,発酵工学,一般講演)
- 2012年, 酵素工学研究会講演会講演要旨集, 68thイオン液体耐性セルラーゼの単離と酵素特性解析
- 2012年, 日本生物工学会大会講演要旨集, 64thイオン液体耐性セルラーゼの探索と機能解析
- 2012年, 日本農芸化学会大会講演要旨集(Web), 2012海洋性超好熱菌Thermotoga neapolitana由来β-1,3-キシラナーゼの耐熱性を導く要因の解析
- 2012年, 日本農芸化学会大会講演要旨集(Web), 2012海洋性超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの構造安定性
- 2012年, 日本農芸化学会大会講演要旨集(Web), 2012麹菌Aspergillus oryzaeを用いたラクダ科動物由来一本鎖抗体可変部位VHH分泌生産系の構築
- (一社)日本癌治療学会, 2011年09月, 日本癌治療学会誌, 46(2) (2), 1046 - 1046, 日本語超音波と酸化チタンを用いた音響力学療法の基礎的検討
- 2011年03月05日, 日本農芸化学会大会講演要旨集, 2011, 47, 日本語Streptomyces griseocarneusスフィンゴミエリナーゼCの速度論的解析とMg2+による活性増強メカニズムの解明
- 日本生物工学会, 2011年, 日本生物工学会大会講演要旨集, 63, 69 - 69, 日本語1Kp11 BGL表層提示大腸菌によるセロビオースを基質としたIPA生産(代謝工学,一般講演)
- 日本生物工学会, 2011年, 日本生物工学会大会講演要旨集, 63, 71 - 71, 日本語1Lp01 生体分子融合ナノ粒子の生物化学工学領域への高度応用に関する研究(生物工学奨励賞(照井賞)受賞講演)
- 日本生物工学会, 2011年, 日本生物工学会大会講演要旨集, 63, 24 - 24, 日本語1Ap14 イオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発(バイオマス・資源・エネルギー工学,一般講演)
- 日本生物工学会, 2011年, 日本生物工学会大会講演要旨集, 63, 28 - 28, 日本語1Bp17 酵母によるバイオマスからのグルタチオンの直接発酵(バイオマス・資源・エネルギー工学,一般講演)
- 日本生物工学会, 2011年, 日本生物工学会大会講演要旨集, 63, 23 - 23, 日本語1Ap13 セルラーゼ分泌酵母によるイオン液体処理バイオマスからのエタノール生産(バイオマス・資源・エネルギー工学,一般講演)
- 日本生物工学会, 2011年, 日本生物工学会大会講演要旨集, 63, 23 - 23, 日本語1Ap12 イオン液体処理が結晶性セルロース酵素糖化に与える影響(バイオマス・資源・エネルギー工学,一般講演)
- 日本生物工学会, 2011年, 日本生物工学会大会講演要旨集, 63, 179 - 179, 日本語2Ip12 組換え酵母による高効率グルタチオン生産(生物化学工学,一般講演)
- 日本生物工学会, 2011年, 日本生物工学会大会講演要旨集, 63, 42 - 42, 日本語1Ep12 麹菌Aspergillus oryzaeによる超好熱菌Thermotoga neapolitana由来耐熱性β-1,4-マンナナーゼの生産(酵素学・酵素工学/タンパク質工学/糖鎖工学,一般講演)
- 日本生物工学会, 2011年, 日本生物工学会大会講演要旨集, 63, 201 - 201, 日本語2Lp13 Cell-SELEX法により選抜したヒト肝臓由来がん細胞に対するDNAアプタマーの評価(生体医用工学・人工臓器/バイオセンシング・分析化学,一般講演)
- 2011年, 日本農芸化学会関西支部講演会講演要旨集, 472nd麹菌Aspergillus oryzaeを用いたラクダ由来一本鎖抗体可変部位VHH分泌生産系の構築
- 2011年, 日本生物工学会大会講演要旨集, 63rd麹菌Aspergillus oryzaeによる超好熱菌Thermotoga neapolitana由来耐熱性β-1,4-マンナナーゼの生産
- 2011年, 日本生物工学会大会講演要旨集, 63rdAffibody提示Bio-nanocapsuleを用いたHER2発現癌細胞へのタンパク質送達システム
- 2011年, 日本生物工学会大会講演要旨集, 63rd麹菌Aspergillus oryzaeを用いたラクダ由来一本鎖抗体可変部位VHHの生産
- 日本生物工学会, 2011年, 日本生物工学会大会講演要旨集, 63, 180 - 180, 日本語2Ip13 Affibody提示Bio-nanocapsuleを用いたHER2発現癌細胞へのタンパク質送達システム(生物化学工学,一般講演)
- フレグランスジャーナル社, 2010年10月, Fragrance journal, 38(10) (10), 39 - 44, 日本語バイオプロダクション次世代農工連携研究と拠点形成の展望 (特集 自然に学ぶ材料開発)
- 2010年09月25日, 日本生物工学会大会講演要旨集, 62nd, 30, 日本語Streptomyces griseocarneus NBRC13471由来スフィンゴミエリナーゼCの大量発現
- 公益社団法人日本生物工学会, 2010年09月25日, 日本生物工学会大会講演要旨集, 22, 30 - 30, 日本語2P-1085 Streptomyces sriseocameus NBRC13471由来スフィンゴミエリナーゼCの大量発現(2a酸素学,酵素工学,一般講演,酵素学,タンパク質工学および酵素工学,伝統の技と先端科学技術の融合)
- 公益社団法人日本生物工学会, 2010年09月25日, 日本生物工学会大会講演要旨集, 22, 30 - 30, 日本語2P-1086 Streptomyces griseocarneus NBRC13471由来スフィンゴミエリナーゼCの大量発現および速度論的解析(2a酸素学,酵素工学,一般講演,酵素学,タンパク質工学および酵素工学,伝統の技と先端科学技術の融合)
- 日本生物工学会, 2010年, 日本生物工学会大会講演要旨集, 22, 35 - 35, 日本語2P-1106 放線菌を用いた様々なタンパクの大量分泌系の構築(3a発酵生理学,発酵工学,一般講演,代謝生理学・発酵生産,伝統の技と先端科学技術の融合)
- 日本生物工学会, 2010年, 日本生物工学会大会講演要旨集, 22, 197 - 197, 日本語2S-Ba05 1細胞アッセイ技術に基づくGPCR解析、アゴニスト探索法の開発(1分子1細胞アッセイ技術に基づく生物工学の最近の潮流,シンポジウム,伝統の技と先端科学技術の融合)
- 日本生物工学会, 2010年, 日本生物工学会大会講演要旨集, 22, 168 - 168, 日本語3P-2082 過酸化チタンナノ粒子及び低線量X線照射を併用した深部ガンの非侵襲的治療法の開発(8a 生体医用工学,人工臓器,一般演題,動物バイオテクノロジー,伝統の技と先端科学技術の融合)
- 日本生物工学会, 2010年, 日本生物工学会大会講演要旨集, 22, 154 - 154, 日本語3P-2043 イオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発(その3)(5c バイオマス,資源,エネルギー工学,一般演題,環境バイオテクノロジー,伝統の技と先端科学技術の融合)
- 日本生物工学会, 2010年, 日本生物工学会大会講演要旨集, 22, 169 - 169, 日本語3P-2084 Cell SELEX法によるヒト肝臓由来がん細胞に選択的に結合するDNAアプタマーの選抜(8a 生体医用工学,人工臓器,一般演題,動物バイオテクノロジー,伝統の技と先端科学技術の融合)
- 日本生物工学会, 2010年, 日本生物工学会大会講演要旨集, 22, 102 - 102, 日本語3P-1180 Affibody提示ナノカプセルを用いたHER2発現癌細胞特異的な薬物送達(6a生物化学工学,一般講演,生物化学工学,伝統の技と先端科学技術の融合)
- 日本生物工学会, 2010年, 日本生物工学会大会講演要旨集, 22, 118 - 118, 日本語2P-2047 セルラーゼ発現バランス最適化酵母を用いたセルロースからのエタノール発酵(5c バイオマス,資源,エネルギー工学,一般演題,環境バイオテクノロジー,伝統の技と先端科学技術の融合)
- 日本生物工学会, 2010年, 日本生物工学会大会講演要旨集, 22, 233 - 233, 日本語3S-Da03 環境・医療応用に向けたバイオ・無機複合ナノ粒子の開発(ナノバイオテクノロジーによる環境への新アプローチ,シンポジウム,伝統の技と先端科学技術の融合)
- 2010年, 日本生物工学会大会講演要旨集, 62nd超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの機能解析
- 2010年, 日本生物工学会大会講演要旨集, 62nd麹菌による超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの高分泌生産
- 2010年, Journal of Applied Glycoscience, 57(Suppl.) (Suppl.)麹菌(Aspergillus oryzae)を宿主とした超好熱菌Thermotoga neapollitana由来耐熱性β-1,3-キシラナーゼの分泌高生産
- 2010年, Journal of Applied Glycoscience, 57(Suppl.) (Suppl.)超好熱菌Thermotoga neapollitana由来耐熱性β-1,3-キシラナーゼの発現および諸性質
- 公益社団法人日本生物工学会, 2009年11月, Journal of bioscience and bioengineering, 108(1) (1), S27, 英語BP-P10 Affibody displaying bionanocapsules for HER2 specific drug delivery(Section II Biopharmaceuticals Production)
- 公益社団法人日本生物工学会, 2009年11月, Journal of bioscience and bioengineering, 108(1) (1), S64, 英語BE-O21 New SELEX strategy for screening of DNA aptamer by AFM(Section V Biomolecular Engineering and Bioseparation)
- 公益社団法人日本生物工学会, 2009年11月, Journal of bioscience and bioengineering, 108(1) (1), S71, 英語BE-P21 Screening of DNA aptamers that recognize amino acid by AFM-SELEX strategy(Section V Biomolecular Engineering and Bioseparation)
- SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S64 - S64, 英語研究発表ペーパー・要旨（国際会議）
- SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S50 - S50, 英語研究発表ペーパー・要旨（国際会議）
- SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S27 - S27, 英語研究発表ペーパー・要旨（国際会議）
- SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S50 - S50, 英語研究発表ペーパー・要旨（国際会議）
- SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S47 - S48, 英語研究発表ペーパー・要旨（国際会議）
- SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S159 - S160, 英語研究発表ペーパー・要旨（国際会議）
- SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S48 - S48, 英語研究発表ペーパー・要旨（国際会議）
- SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S51 - S52, 英語研究発表ペーパー・要旨（国際会議）
- SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S51 - S51, 英語研究発表ペーパー・要旨（国際会議）
- SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S49 - S49, 英語研究発表ペーパー・要旨（国際会議）
- SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S48 - S49, 英語研究発表ペーパー・要旨（国際会議）
- SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S52 - S52, 英語研究発表ペーパー・要旨（国際会議）
- SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S36 - S37, 英語研究発表ペーパー・要旨（国際会議）
- SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S71 - S71, 英語研究発表ペーパー・要旨（国際会議）
- SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S43 - S43, 英語[査読有り]研究発表ペーパー・要旨（国際会議）
- SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S53 - S53, 英語[査読有り]研究発表ペーパー・要旨（国際会議）
- SOC BIOSCIENCE BIOENGINEERING JAPAN, 2009年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S53 - S53, 英語[査読有り]研究発表ペーパー・要旨（国際会議）
- ティー・アイ・シィー, 2009年06月, マテリアルインテグレ-ション, 22(6) (6), 8 - 11, 日本語光触媒ナノ粒子と超音波技術をカップリングしたがん治療法 (特集 マイクロ・ナノバブル(2)マイクロバブルとソノプロセス)
- 日本生物工学会, 2009年, 日本生物工学会大会講演要旨集, 21, 57 - 57, 日本語1Ep22 癌細胞標的化を目指したAffibody提示ナノカプセルの開発(タンパク質工学,一般講演)
- 日本生物工学会, 2009年, 日本生物工学会大会講演要旨集, 21, 170 - 170, 日本語2La01 生デンプンから高効率にエタノールを生産する新規酵母の創製(生物化学工学,一般講演)
- 日本生物工学会, 2009年, 日本生物工学会大会講演要旨集, 21, 172 - 172, 日本語2La11 Porinを用いたコリネ菌新規細胞表層提示技術の開発(生物化学工学,一般講演)
- 日本生物工学会, 2009年, 日本生物工学会大会講演要旨集, 21, 173 - 173, 日本語2Lp02 遺伝子組換え麹菌を用いたタンパク質大量分泌生産技術の開発(生物化学工学,一般講演)
- 化学工学会, 2008年11月05日, 化学工学 = Chemical engineering, 72(11) (11), 636 - 638, 日本語二酸化チタン/超音波触媒法とその医療応用
- 2008年11月05日, 石油・石油化学討論会講演要旨, 38th, 211, 日本語Streptomyces griseocameus NBRC13471由来スフィンゴミエリナーゼC遺伝子のクローニングと発現
- 2008年10月30日, バイオイメージング, 17(2) (2), 110 - 111, 日本語特異性改変型バイオナノカプセルを用いたバイオイメージング及びDDSへの応用
- AMER CHEMICAL SOC, 2008年08月, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 236, 英語ORGN 297-Phospholipid conversion in ionic liquid by phospholipase D研究発表ペーパー・要旨（国際会議）
- 金沢大学環日本海域環境研究センター = Institute of Nature and Environmental Technology, Kanazawa University, Japan, 2008年07月31日, 年報 金沢大学環日本海域環境研究センター = K-INET Kanazawa University, 2007, 129 - 134, 日本語機能性二酸化チタン・ナノ粒子を用いたがん治療への基礎研究
- 金沢大学環日本海域環境研究センター = Institute of Nature and Environmental Technology, Kanazawa University, Japan, 2008年07月31日, 年報 金沢大学環日本海域環境研究センター = K-INET Kanazawa University, 2007, 135 - 140, 日本語二酸化チタン/超音波触媒法による過酸化水素生成機構の解析
- Titanium dioxide (TiO2)/polyacrylic acid (PAA) (TiO2/PAA) particles were formed by mixing PAA and an acidic solution of TiO2 nanoparticles in dimethylformamide (DMF) followed by heat treatment. TEM and particle analysis showed that the resulting particles had a narrow size distribution. The colloid was very stable and aggregation was not observed over a wide pH range (3-9) or at high salt concentration. The residual carboxylic acid of PAA could be modified via EDC/NHS activation to form an amide bond with a protein. An antibody was attached to the hybrid nanoparticle and specific binding to antigen was monitored by surface plasmon resonance. The results suggest that TiO2/PAA nanoparticles are candidates as the base component of a photocatalytic system with potential for substrate selectivity. (C) 2008 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, 2008年06月, COLLOIDS AND SURFACES B-BIOINTERFACES, 64(1) (1), 10 - 15, 英語
- 化学工業社, 2008年02月, ケミカルエンジニヤリング, 53(2) (2), 140 - 144, 日本語微生物を用いたバイオ・リファイナリー戦略--バイオマスから燃料・化学品の生産 (特集 バイオマス利用技術の展望)
- 日本生物工学会, 2008年, 日本生物工学会大会講演要旨集, 20, 184 - 184, 日本語2Gp20 遺伝子組み換え放線菌を用いたセルラーゼ発現システムの構築(酵素学,酵素工学,タンパク質工学,一般講演)
- 日本生物工学会, 2008年, 日本生物工学会大会講演要旨集, 20, 156 - 156, 日本語1Fp15 Streptomyces griseocarneus NBRC13471由来スフィンゴミエリナーゼCの遺伝子クローニングと発現(酵素学,酵素工学,タンパク質工学,一般講演)
- 日本生物工学会, 2008年, 日本生物工学会大会講演要旨集, 20, 158 - 158, 日本語2Fa01 リン脂質代謝酵素のイオン液体中での反応解析(酵素学,酵素工学,タンパク質工学,一般講演)
- 日本生物工学会, 2008年, 日本生物工学会大会講演要旨集, 20, 60 - 60, 日本語2Aa08 無蒸煮デンプンから効率的にエタノールを生産する高機能酵母の創製(生物化学工学,一般講演)
- 2008年, 酵素工学研究会講演会講演要旨集, 60th, 71, 日本語Streptomyces griseocarneus NBRC13471由来スフィンゴミエリナーゼCの遺伝子クローニングと発現
- Streptomyces griseocarneus NBRC13471由来スフィンゴミエリナーゼC遺伝子のクローニングと発現系構築<i>S. griseocarneus</i> NBRC13471スフィンゴミエリナーゼC遺伝子<i>smc</i>をクローニングした。次に、発現ベクターpUC702に<i>smc</i>を導入し、<i>Streptoverticillium cinnamoneum</i>を宿主として発現を行った。その結果、形質転換体の培養上清液中に約100 mg/mlの本酵素を分泌生産させることができた。しかしながら、培養上清中の酵素比活性は3 mU/mgと元株の約100分の１であった。公益社団法人 石油学会, 2008年, 石油学会 年会・秋季大会講演要旨集, 2008(0) (0), 142 - 142, 日本語
- 日本工業出版, 2007年09月, 超音波techno, 19(5) (5), 41 - 45, 日本語二酸化チタン・超音波触媒法による細胞死誘導 (特集 ソノケミストリーの最新動向(2))
- ELSEVIER SCIENCE BV, 2007年09月, JOURNAL OF BIOTECHNOLOGY, 131(2) (2), S185 - S186, 英語研究発表ペーパー・要旨（国際会議）
- ELSEVIER SCIENCE BV, 2007年09月, JOURNAL OF BIOTECHNOLOGY, 131(2) (2), S237 - S238, 英語研究発表ペーパー・要旨（国際会議）
- 日本工業出版, 2007年07月, 超音波techno, 19(4) (4), 45 - 49, 日本語二酸化チタン・超音波触媒法を応用した有害微生物殺菌システム (特集 ソノケミストリーの最新動向(1))
- 日本工業出版, 2007年07月, 超音波techno, 19(4) (4), 39 - 44, 日本語二酸化チタン・超音波触媒法によるOHラジカルの生成と溶存希ガスの促進効果 (特集 ソノケミストリーの最新動向(1))
- 日本空気清浄協会, 2007年, 空気清浄, 45(1) (1), 24 - 31, 日本語二酸化チタン・超音波触媒法の環境浄化、殺菌への応用 (特集 超音波技術の最近)
- 日本生物工学会, 2007年, 日本生物工学会大会講演要旨集, 19, 213 - 213, 日本語2J11-1 放線菌Streptomyces lividansにおける分泌シグナルのタンパク生産に及ぼす影響解析(生態工学,生物化学工学,一般講演)
- 日本生物工学会, 2007年, 日本生物工学会大会講演要旨集, 19, 82 - 82, 日本語2C10-3 放線菌を宿主とするリン脂質代謝酵素の変異体解析(酵素学・酵素工学・タンパク質工学,一般講演)
- 日本生物工学会, 2006年, 日本生物工学会大会講演要旨集, 18, 142 - 142, 日本語2H12-5 生体ストレスマーカー分子8OH-dGを認識するDNAアプタマーに関する研究(遺伝子工学,核酸工学,一般講演)
- 日本生物工学会, 2006年, 日本生物工学会大会講演要旨集, 18, 202 - 202, 日本語2K12-4 二酸化チタン・超音波触媒法によるがん細胞傷害と増殖抑制(生体医用工学,人工臓器/動物細胞工学,動物組織培養,一般講演)
- 日本生物工学会, 2006年, 日本生物工学会大会講演要旨集, 18, 203 - 203, 日本語2K12-5 組織認識能をもつ二酸化チタン内包リポソームの創製(生体医用工学,人工臓器/動物細胞工学,動物組織培養,一般講演)
- 日本生物工学会, 2006年, 日本生物工学会大会講演要旨集, 18, 212 - 212, 日本語1L10-5 放線菌を用いたホスホリパーゼD酵素の遺伝子組み換え生産の培養特性に関する研究(生物化学工学,一般講演)
- ティ-・アイ・シ-, 2005年03月, マテリアルインテグレ-ション, 18(3) (3), 31 - 36, 日本語二酸化チタンへの超音波照射によって発生するラジカルの応用 (特集 いろいろつかえるソノケミストリー(1))
- 化学工業社, 2005年02月, 化学工業, 56(2) (2), 106 - 111, 日本語二酸化チタン・超音波照射法による大腸菌殺菌メカニズム (特集/ソノケミストリーの新しい進展)
- 日本生物工学会, 2005年, 日本生物工学会大会講演要旨集, 17, 153 - 153, 日本語2E11-2 リン脂質代謝酵素ホスホリパーゼDの蓋構造の活性相関に関する研究(生物化学工学,一般講演)
- 日本生物工学会, 2005年, 日本生物工学会大会講演要旨集, 17, 152 - 152, 日本語2E10-3 放線菌Streptomyces cinnamoneum由来発現プロモーターを用いたタンパク質発現系構築に関する研究(生物化学工学,一般講演)
- バイオインダストリー協会, 2004年12月01日, バイオサイエンスとインダストリー = Bioscience & industry, 62(12) (12), 809 - 812, 日本語ホスホリパｰゼDの生産とリン脂質合成への適用
- 日本生物工学会, 2004年, 日本生物工学会大会講演要旨集, 16, 146 - 146, 日本語3C11-4 生理活性物質を認識するDNAアプタマーの探索とその機能評価(遺伝子工学・核酸工学,一般講演)
- 日本生物工学会, 2003年, 日本生物工学会大会講演要旨集, 15, 71 - 71, 日本語2B10-3 放線菌由来リン脂質代謝酵素の変異体の機能解析
- 日本生物工学会, 2002年, 日本生物工学会大会講演要旨集, 14, 171 - 171, 日本語927 有機溶媒感受性プロモーターを用いた水質評価法の構築(環境浄化・修復・保全技術,一般講演)
- 日本生物工学会, 2002年, 日本生物工学会大会講演要旨集, 14, 112 - 112, 日本語672 リン脂質代謝酵素ホスホリパーゼDの遺伝子工学的手法による触媒機能解析(酵素・酵素工学・タンパク質工学,一般講演)
- 日本生物工学会, 2000年, 日本生物工学会大会講演要旨集, 12, 183 - 183, 日本語809 遺伝子工学的手法による活性変異型ホスホリパーゼDの構築と解析
- 日本生物工学会, 1999年, 日本生物工学会大会講演要旨集, 11, 92 - 92, 日本語524 放線菌Stv. Cinnamoneum由来PLDの発現系構築と触媒機構解析
- 日本生物工学会, 1998年, 日本生物工学会大会講演要旨集, 10, 166 - 166, 日本語832 放線菌由来膜局在型ホスホリパーゼDの精製とキャラクタリゼーション
- 日本生物工学会, 1997年, 日本生物工学会大会講演要旨集, 9, 134 - 134, 日本語415 放射菌由来ホスホリパーゼDの精製とクローニング
- 日本生物工学会, 1996年, 日本生物工学会大会講演要旨集, 8, 189 - 189, 日本語645 放線菌における情報関連蛋白質PLDの研究
- 共著, Springer Nature, 2019年05月, 英語Yeast Cell Surface Engineering : "Energy Production: Biodiesel"学術書
- 共著, Elsevier, 2018年03月, 英語A cancer treatment strategy that combines the use of inorganic/biocomplex nanoparticles with conventional radiation therapy, Nanoparticle Technology Handbook学術書
- 共著, Springer, 2015年04月, 英語Green Processes for Nanotechnology学術書
- 共著, テックタイムス, 2014年08月, 日本語紙パルプ産業と環境 2015学術書
- 共著, 丸善出版, 2014年01月, 日本語化学便覧 応用化学編学術書
- 共著, コロナ社, 2013年09月, 日本語基礎から学ぶ生物化学工学演習学術書
- 共著, シーエムシー出版, 2013年05月, 日本語リサイクルバイオテクノロジーの最前線, 第I編 第1章, 1 未来型CBP法によるバイオエタノール生産技術の展開学術書
- 共著, シーエムシー出版, 2012年12月, 日本語次世代医薬開発に向けた抗体工学の最前線 （第Ⅱ編 抗体の改変技術，第4章 親和性の向上，第4節 「酵母による抗体フラグメントおよび抗体様結合性タンパク質の改変技術」）学術書
- 共著, シーエムシー出版, 2010年12月, 日本語エコバイオリファイナリー学術書
- 共著, シーエムシー出版, 2010年04月, 日本語グリーンバイオケミストリーの最前線学術書
- 共著, シーエムシー出版, 2010年03月, 日本語シングルセル解析の最前線 （第３章 細胞内生体分子群の実測定量解析，第4節 「フローサイトメトリーとGFPレポーターによるG蛋白質シグナルのシングルセル解析」）学術書
- 共著, シーエムシー出版, 2009年04月, 日本語第二世代バイオ燃料の開発と応用展開学術書
- 単著, シーエムシー出版, 2008年06月, 日本語微生物によるものづくり学術書
- 単著, 学進出版, 2008年02月, 日本語二酸化チタンと超音波で殺菌?!学術書
- 共著, 関西化学機械製作株式会社創立60周年記念出版, 2008年02月, 日本語進化・増殖する化学工学学術書
- 共著, ケミカルエンジニアリング, 2008年, 日本語微生物を用いたバイオ・リファイナリー戦略:バイオマスからの燃料・化学品の生産一般書・啓蒙書
- 共著, 超音波テクノ, 2007年09月, 日本語二酸化チタン・超音波触媒法による細胞死誘導学術書
- 共著, 超音波テクノ, 2007年07月, 日本語二酸化チタン・超音波触媒法を応用した有害微生物殺菌システム学術書
- 共著, 超音波テクノ, 2007年07月, 日本語二酸化チタン・超音波触媒法によるOHラジカルの生成と溶存希ガスの促進効果学術書
- 共著, 空気清浄, 2007年05月, 日本語二酸化チタン・超音波触媒法の環境浄化、殺菌への応用学術書
- 共著, 最新医学, 2006年06月, 日本語ナノテクノロジーによる新規がん治療法.学術書
- 単著, 生物工学会誌, 2006年01月, 日本語二酸化チタンと超音波で殺菌?学術書
- 共著, NTS, 2005年04月, 日本語超音波利用技術集成「超音波と二酸化チタンを用いた有害微生物の殺菌」学術書
- 共著, マテリアルインテグレーション, 2005年03月, 日本語二酸化チタンへの超音波照射によって発生するラジカルの応用学術書
- 共著, 化学工業, 2005年02月, 日本語二酸化チタン・超音波照射による大腸菌殺菌メカニズム学術書
- 共著, バイオサイエンスとインダストリー, 2004年12月, 日本語ホスホリパーゼDの生産とリン脂質合成への適用学術書
- 共著, Recent Research Developments in Physiology, 2003年, 英語Stress-induced serotonin release in the hypothalamus and suppression of food intake in rats学術書
- 共著, 三恵社, 2002年03月, 日本語テッシュエンジニアリングの展望学術書
- 単著, INSEF, 2002年, 英語Functional analysis of phospholipase D and its application for phospholipids synthesis学術書
- 単著, 生物工学会誌, 2000年07月, 日本語エコエネルギー学術書
- 日本農芸化学会2020年度大会メタボローム解析が明らかにする低酸素濃度におけるコリネ型細菌の芳香族化合物代謝促進機構と代謝改変によるソルガム搾汁液からの物質生産
- 第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議油脂酵母Lipomyces starkeyi の遺伝子的改変による長鎖長脂肪酸の生産口頭発表（一般）
- 第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議日本酒生産にヒントを得た麹菌酵素カクテルと酵母を用いた高純度希少糖生産口頭発表（一般）
- 第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議新奇な好熱性放線菌Streptomyces thermoviolaceus を用いたタンパク質生産株の開発口頭発表（一般）
- 第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議酵母カルチャーコレクションからのバイオエタノール生産のための優良酵母の選抜と評価口頭発表（一般）
- 第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議好冷性海洋細菌Psychroflexus torquis 由来β-1,3-キシラナーゼの酵素化学的諸性質口頭発表（一般）
- 第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議好熱性放線菌Streptomyces thermoviolaceus による物質生産口頭発表（一般）
- 第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議基質酵素反応予測のための 機械学習に基づく予測モデルの構築方法の探索と評価口頭発表（一般）
- 第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議過酸化チタンナノ粒子 (PAA -TiOx )の 細胞内傷害口頭発表（一般）
- 第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議イオン液体で前処理した木質系バイオマス中の多糖およびリグニンのリファイナリー口頭発表（一般）
- 第21回化学工学会学生発表会, 2019年03月, 日本語, 京都大学桂キャンパス, 国内会議新奇な好熱性放線菌Streptomyces thermoviolaceusを用いたタンパク質生産株の開発ポスター発表
- 第21回化学工学会学生発表会, 2019年03月, 日本語, 京都大学桂キャンパス, 国内会議完全分散型麹菌のプロテオーム解析ポスター発表
- 日本農芸化学会2019年度大会, 2019年03月, 日本語, 東京農業大学 世田谷キャンパス, 国内会議ピルビン酸代謝フロー改変を用いた 2,3-ブタンジオール 高生産麹菌の構築ポスター発表
- 日本農芸化学会2019年度大会, 2019年03月, 日本語, 東京農業大学 世田谷キャンパス, 国内会議ナノポアシーケンサを用いた遺伝子組換え麹菌のゲノミ クスおよびトランスクリプトミクスポスター発表
- 第21回化学工学会学生発表会, 2019年03月, 日本語, 京都大学桂キャンパス, 国内会議PEG化チタンナノ粒子の種々の表面修飾による特性解析ポスター発表
- 18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), 2019年, APCChE, Sapporo Convention CenterBio-refinery strategy by collaboration Asian's partnership
- 18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), 2019年, APCChE, Sapporo Convention CenterDirect increase of intracellular hydrogen peroxide and enhancement for radiosensitivity with polyacrylic acid-modified titanium peroxide nanoparticles
- 第１８回糸状菌分子生物学コンファレンス, 2018年11月, 日本語, シティホールプラザ「アオーレ長岡」, 国内会議乳酸生産麹菌のピルビン酸ミトコンドリアキャリアタンパク質欠損における代謝変動ポスター発表
- 第13回ムギ類研究会, 2018年11月, 日本語, 横浜市立大学木原生物学研究所, 国内会議外来遺伝子発現用のコムギ細胞外排出シグナルと乾燥誘導性プロモーターの効果ポスター発表
- 第１８回糸状菌分子生物学コンファレンス, 2018年11月, 日本語, シティホールプラザ「アオーレ長岡」, 国内会議黄麹菌における汎用性高発現プロモーターポスター発表
- 第１８回糸状菌分子生物学コンファレンス, 2018年11月, 日本語, シティホールプラザ「アオーレ長岡」, 国内会議マルチコピー遺伝子導入黄麹菌のゲノム構造解析ポスター発表
- 異業種交差点Ⅱ, 2018年11月, 日本語, 新化学技術推進協会, 一橋講堂 2階中会議室, 国内会議バイオマスから基礎化学品原料をつくる(2)ポスター発表
- The 24th Symposium of Young Asian Biological Engineers’ Community (YABEC 2018), 2018年11月, 英語, National Taiwan University, 国際会議Target cancer therapy by inorganic nanoparticle口頭発表（一般）
- The 24th Symposium of Young Asian Biological Engineers’ Community (YABEC 2018), 2018年11月, 英語, National Taiwan University, 国際会議Caffeic acid production from lignocellulosic biomass by metabolically engineered Eshrichaia coli口頭発表（一般）
- 第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議油脂酵母Lipomyces starkeyi D35株の 繰り返し発酵による脂肪酸組成変化解析ポスター発表
- 第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議免疫クリアランスを回避する生体膜由来ドラッグデリバリーキャリアの開発ポスター発表
- 第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議乳酸高生産性黄麹菌における乳酸生産フラックス強化による乳酸生産性の向上ポスター発表
- 第70回日本生物工学会大会, 2018年09月, 日本語, 日本生物工学会, 関西大学 千里山キャンパス, 国内会議二次代謝系の動的特性解析における大過剰化合物測定の有無の影響の数理解析ポスター発表
- 第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議組換えコリネ型細菌を用いた3-アミノ-4-ヒドロキシ安息香酸の発酵生産における酸素制限による 促進作用ポスター発表
- 第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議最少量の低毒性イオン液体を用いた前処理に基づく木質系バイオマスリファイナリーポスター発表
- 第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議最少量の低毒性イオン液体を用いたバイオマスからバイオ燃料へのHigh-loadingワンポット変換ポスター発表
- 第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議麹菌内で同調的発現挙動を示すプロモーターを用いたタンパク質生産挙動の解析ポスター発表
- 第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議好熱性放線菌Streptomyces thermoviolaceus の培養特性解析ポスター発表
- 第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議原子間力顕微鏡を用いたB型肝炎感染機構の解明ポスター発表
- 第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議機械学習を用いた酵素のEC number予測ポスター発表
- 第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議バイオマス分解酵素の大規模スクリーニングで見出した希少放線菌ポスター発表
- 第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議ナノポアシーケンサを用いた遺伝子組換え麹菌ゲノム上の長鎖タンデムリピート構造の解析ポスター発表
- 第70回日本生物工学会大会, 2018年09月, 英語, 関西大学 千里山キャンパス, 国内会議The enhancement of culture conditions for high cell and lipid production of oleaginous yeast Lipomyces starkeyii D35ポスター発表
- 第70回日本生物工学会大会, 2018年09月, 英語, 関西大学 千里山キャンパス, 国内会議Selection of oleaginous yeast capable of high lipid accumulating during challenges from inhibitory chemical complexesポスター発表
- 第70回日本生物工学会大会, 2018年09月, 英語, 関西大学 千里山キャンパス, 国内会議Repeated ethanol fermentation from membrane-concentrated sweet sorghum juice by flocculating yeast Saccharomyces cerevisiaeポスター発表
- 第70回日本生物工学会大会, 2018年09月, 英語, 関西大学 千里山キャンパス, 国内会議Preparation and characterisation of robust whole cell biocatalyst from Aspergillus oryzae expressing Fusarium heterosporum lipase immobilized onto activated carbon for biodiesel synthesisポスター発表
- 第70回日本生物工学会大会, 2018年09月, 英語, 関西大学 千里山キャンパス, 国内会議Optimization on biodiesel synthesis from palm oil mill effluent (POME) by integrated strategy with lipasecatalyzed ethanolysisポスター発表
- 第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議mRNAサイレンシングを用いた過酸化チタンナノ粒子の放射線増感機序解明ポスター発表
- 第70回日本生物工学会大会, 2018年09月, 英語, 関西大学 千里山キャンパス, 国内会議Improvement of mass mixing by Max-Blend and anchor shape impeller in the high-solid state scarification of kraft pulpポスター発表
- 第70回日本生物工学会大会, 2018年09月, 英語, 関西大学 千里山キャンパス, 国内会議Effect of immobilized Chlorella Sorokiniana culture on lipid accumulation and CO2 sequestrationポスター発表
- 第70回日本生物工学会大会, 2018年09月, 英語, 関西大学 千里山キャンパス, 国内会議Dilute maleic acid pretreatment of sugarcane bagasse and oil palm empty fruit bunch fiber for enhanced enzymatic digestibilityポスター発表
- 第34回日本DDS学会学術集会, 2018年06月, 日本語, 長崎ブリックホール, 国内会議免疫クリアランスの回避を目指した生体膜由来キャリアの開発ポスター発表
- 第34回日本DDS学会学術集会, 2018年06月, 日本語, 長崎ブリックホール, 国内会議新規放射線増感剤としての過酸化チタンナノ粒子の物性解析ポスター発表
- 第34回日本DDS学会学術集会, 2018年06月, 日本語, 長崎ブリックホール, 国内会議過酸化チタンナノ粒子の併用による放射線増感治療法の開発ポスター発表
- 第7回JACI/GSCシンポジウム, 2018年06月, 日本語, 新化学技術推進協会, ANA CROWNE PLAZA KOBE, 国内会議バイオマスから基礎化学品原料をつくるポスター発表
- The 8th International Conference on Algal Biomass, Biofuels and Bioproducts (AlgalBBB 2018), 2018年06月, 英語, Motif Seattle, 国際会議Converting Chlamydomonas sp. JSC4 lipids to biodiesel via biocatalysis with Fusarium heterosporum lipase-expressing Aspergillus oryzae whole-cellポスター発表
- 化学工学会，第83年会, 2018年03月, 日本語, 関西大学, 国内会議多孔体粒子を用いた固定化培養槽の撹拌性能調査ポスター発表
- 日本農芸化学会2018年度大会, 2018年03月, 日本語, 日本農芸化学会, 名古屋市, 国内会議酸素をスイッチとしたコリネ型細菌中央代謝経路の切り替えポスター発表
- 日本農芸化学会2018年度大会, 2018年03月, 日本語, 日本農芸化学会, 名古屋市, 国内会議麹菌ミトコンドリア; ピルビン酸キャリアタンパク質欠損株におけるピルビン酸代謝フラックス改変による有用化合物生産ポスター発表
- 第3回デザイン生命工学研究会, 2018年03月, 日本語, デザイン生命工学研究, 沖縄県国頭郡今帰仁村, 国内会議酵素改変を指向したin silico解析ポスター発表
- 日本農芸化学会2018年度大会, 2018年03月, 日本語, 日本農芸化学会, 名古屋市, 国内会議リグノセルロース系バイオマスを包括的に利用したコリネ型細菌によるバイオモノマー生産ポスター発表
- 日本化学会第98春季年会2018, 2018年03月, 日本語, 日本化学会, 船橋市, 国内会議リグノセルロースナノファイバー表面のリグニン分布解析手法の開発ポスター発表
- 日本農芸化学会2018年度大会, 2018年03月, 日本語, 日本農芸化学会, 名古屋市, 国内会議Saccharomyces cerevisiae酵母におけるCandida boidinii由来キシロース発酵代謝系の導入効果ポスター発表
- 第13回バイオマス科学会議, 2018年01月, 英語, 日本エネルギー学会, 仙台市, 国際会議同時糖化発酵プロセスを利用した紙パルプを原料とするカフェ酸の生産口頭発表（一般）
- 5th Asian Conference on Biomass Science, 2018年01月, 英語, 日本エネルギー学会, 仙台市, 国際会議Efficient Xylitol Production from Kraft Pulp by a Cell Surface Engineered Strain ofSaccharomyces cerevisiaeポスター発表
- 第12回ムギ類研究会, 2017年12月, 日本語, 京都大学, 国内会議麦わらのバイオマス利用に関するパンコムギ系統間差異ポスター発表
- 第17回糸状菌分子生物学コンファレンス, 2017年11月, 日本語, 糸状菌分子生物学研究会, 佐賀市, 国内会議麹菌のミトコンドリア局在ピルビン酸キャリアタンパク質欠損による乳酸生産性の向上口頭発表（一般）
- 第17回糸状菌分子生物学コンファレンス, 2017年11月, 日本語, 糸状菌分子生物学研究会, 佐賀市, 国内会議麹菌の co-transformation 時のコピー数多型性に関する解析口頭発表（一般）
- 第7回CSJ化学フェスタ2017, 2017年10月, 日本語, 日本化学会, 東京都, 国内会議リグニン残量を制御したセルロースから得られた各種ナノファイバーの構造と物性ポスター発表
- The 23th Symposium of Young Asian Biochemical Engineers' Communyty (YABEC2017), 2017年10月, 英語, Xi'an, China, 国際会議In situ detection of an arming yeast cellulase using AFM[招待有り]口頭発表（招待・特別）
- 第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議発酵阻害物質耐性Candida boidinii K212のキシロース発酵の解析ポスター発表
- 第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議発酵阻害耐性酵母由来強力なENO1プロモーターの発現解析ポスター発表
- 第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議酸素をスイッチとした二つの樹脂原料の個別生産ポスター発表
- 第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議麹菌による複数遺伝子の同時高発現を可能にするプロモーターセットの開発ポスター発表
- 第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議高度膜分離技術によるソルガム搾汁液からのスクロース分離および連続的エタノール生産口頭発表（一般）
- 第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議The establishment high cell density culture of oleaginous Lipomyces starkeyi D35 for high cell and lipid productionポスター発表
- 第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議High Production of single cell oil from glucose and xylose using oleaginous yeast Lipomyces starkeyiポスター発表
- 第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議Exploration of potential indigenous marine actinomycetes from indonesia soil producing enzyme lignocellulosic for biorefinery applicationポスター発表
- 第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議Enhanced cell surface engineering of Saccharomyces cerevisiae and optimization of the fermentation process for efficient xylitol production from kraft pulp口頭発表（一般）
- 第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議B型肝炎ウイルス由来ペプチドを用いたAFMによる肝細胞の受容体検出ポスター発表
- 第63回高分子研究発表会(神戸), 2017年07月, 日本語, 高分子学会関西支部, 神戸市, 国内会議リグニン量を制御したセルロースナノファイバーの構造と物性口頭発表（一般）
- 平成29年度繊維学会年次大会, 2017年06月, 日本語, 繊維学会, 東京都, 国内会議リグニン含有量を異にするセルロースから得られた各種ナノファイバーの構造と物性ポスター発表
- 日本農芸化学2017年度大会, 2017年03月, 日本語, 日本農芸化学会, 京都市, 国内会議組換え大腸菌によるグルコースを原料とする4-アミノ 桂皮酸の直接発酵生産口頭発表（一般）
- 第19回化学工学会学生発表会, 2017年03月, 日本語, 化学工学会関西支部, 豊中市, 国内会議肝細胞認識ペプチドを用いた AFMによる肝癌細胞の力学的評価口頭発表（一般）
- 日本農芸化学2017年度大会, 2017年03月, 日本語, 日本農芸化学会, 京都市, 国内会議遺伝子組み換え麹菌培養上清カクテルを用いた希少糖高生産プロセスの開発口頭発表（一般）
- 第19回化学工学会学生発表会, 2017年03月, 日本語, 化学工学会関西支部, 豊中市, 国内会議バイオマスの効率的な分解を志向したマルチセルラーゼ高発現麹菌の開発口頭発表（一般）
- 第２回デザイン生命工学研究会大会, 2017年03月, 日本語, デザイン生命工学研究会, 神戸市, 国内会議デザインされた生体分子の触診デバイス口頭発表（一般）
- 日本農芸化学2017年度大会, 2017年03月, 日本語, 日本農芸化学会, 京都市, 国内会議コリネ型細菌を用いたフェルラ酸からプロトカテク酸の生産口頭発表（一般）
- 第19回化学工学会学生発表会, 2017年03月, 日本語, 化学工学会関西支部, 豊中市, 国内会議REDOXバランスにおける発酵阻害耐性酵母由来新規TDHxの影響口頭発表（一般）
- 日本作物学会講演会, 2017年, 日本語, 国内会議デンプン合成制御因子CRCTが稲わらを用いたバイオエタノール生産に及ぼす効果ポスター発表
- 第16回糸状菌分子生物学コンファレンス, 2016年11月, 日本語, 糸状菌分子生物学研究会, 宇治市, 国内会議代謝改変およびフラックス強化した黄麹菌でのデンプンからの乳酸生産口頭発表（一般）
- 第16回糸状菌分子生物学カンファレンス, 2016年11月, 日本語, 宇治おうばくプラザ, 国内会議麹菌におけるゲノムを切らずに書き換える新規ゲノム編集ツールの確立ポスター発表
- 第16回糸状菌分子生物学コンファレンス, 2016年11月, 日本語, 糸状菌分子生物学研究会, 宇治市, 国内会議麹菌におけるゲノムを切らずに書き換える新規ゲノム編集ツールの確立口頭発表（一般）
- 第16回糸状菌分子生物学コンファレンス, 2016年11月, 日本語, 糸状菌分子生物学研究会, 宇治市, 国内会議黄麹菌 Aspergillus oryzae による機能性オリゴ糖の生産口頭発表（一般）
- 第16回糸状菌分子生物学コンファレンス, 2016年11月, 日本語, 糸状菌分子生物学研究会, 宇治市, 国内会議マルチセルラーゼ発現黄麹菌の培養条件によるセルラーゼ生産性の違い口頭発表（一般）
- 第61回リグニン討論会, 2016年10月, 日本語, リグニン討論会実行委員会, 宇治市, 国内会議疎水性アルコールを用いた植物バイオマスの効率的な成分分離法の開発口頭発表（一般）
- The 22th Symposium of Young Asian Biochemical Engineers' Communyty, 2016年10月, 英語, 生物工学会, 宮崎市, 国際会議Mapping of endoglucanases displayed on a surface of yeast cell catalyst using atomic force microscopy口頭発表（一般）
- 第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議転写因子改変放線菌を用いた異種タンパク質生産口頭発表（一般）
- 第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議担癌マウスを用いたポリアクリル酸修飾過酸化チタンナノ粒子の生体内分布の解明口頭発表（一般）
- 化学工学会第48回秋季大会, 2016年09月, 日本語, 化学工学会, 徳島市, 国内会議新規放射線増感治療に向けた過酸化チタンナノ粒子のマウス生体内分布特性の調査口頭発表（一般）
- セラミック協会第 29 回秋季シンポジウム, 2016年09月, 日本語, セラミック協会, 東広島市, 国内会議酸化チタン類ナノ粒子と物理的外部刺激を併用した新規がん治療の提案口頭発表（一般）
- 第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議三種セルラーゼ共・強発現麹菌によるセルロースからのコウジ酸生産口頭発表（一般）
- 第34回日本植物細胞分子生物学会（上田）大会, 2016年09月, 日本語, 日本植物細胞分子生物学会, 長野県上田市, 国内会議原料となる植物バイオマスの種類がセルロー スナノファイバーの強度に与える影響の解析口頭発表（一般）
- 第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議凝集性酵母における新規な阻害剤耐性機構の解明口頭発表（一般）
- 第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議遺伝子組換え大腸菌を用いたフェニル乳酸生産に影響を与える遺伝子の発現解析口頭発表（一般）
- 第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議σ因子欠損放線菌の3,4-AHBA生産への影響口頭発表（一般）
- 第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議バイオナノカプセルとリポソームの複合粒子によるin vivoでの抗腫瘍効果口頭発表（一般）
- 化学工学会第48回秋季大会, 2016年09月, 日本語, 化学工学会, 徳島市, 国内会議ナノ粒子とX線照射の併用による低侵襲がん治療法の開発口頭発表（一般）
- 第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議コリスミ酸ピルビン酸リアーゼ発現コリネ型細菌を用いたプロトカテク酸の生産口頭発表（一般）
- 化学工学会第48回秋季大会, 2016年09月, 日本語, 化学工学会, 徳島市, 国内会議アルコールの添加が希硫酸前処理の効率に与える影響の解析口頭発表（一般）
- 生物工学若手研究者の集い夏のセミナー2016, 2016年07月, 日本語, 生物工学会, 府中市, 国内会議膵臓がん治療に向けた新規放射線増感剤としての過酸化チタンナノ粒子のin vitro評価ポスター発表
- 生物工学若手研究者の集い夏のセミナー2016, 2016年07月, 日本語, 生物工学会, 府中市, 国内会議担癌マウスを用いたポリアクリル酸修飾過酸化チタンナノ粒子の生体内分布の解明ポスター発表
- 生物工学若手研究者の集い夏のセミナー2016, 2016年07月, 日本語, 生物工学会, 府中市, 国内会議HER2受容体発現細胞を標的としたDDSキャリアの抗腫瘍効果の評価ポスター発表
- 生物工学若手研究者の集い夏のセミナー2016, 2016年07月, 日本語, 生物工学会, 府中市, 国内会議AFMで診る・識る・釣る ～核酸・タンパク・細胞の研究～ポスター発表
- 第5回 JACI/GSCシンポジウム, 2016年06月, 日本語, 公益社団法人 新化学技術推進協会, 神戸市, 国内会議ブタノール/硫酸法によりリグニン含有量を調節したリグノセルロースナノファイバーシートの物性ポスター発表
- 第5回JACI GSCシンポジウム, 2016年06月, 日本語, 新化学技術推進協会, 神戸市, 国内会議アルコールの添加が希硫酸前処理の効率に与える影響の解析ポスター発表
- Metabolic Engineering 11, 2016年06月, 英語, Metabolic Engineering 11, 淡路市, 国際会議Optimization of Displayed Cellulases in Yeast Suitable for Ionic Liquid Pretreated Biomass by Cocktail δ Integration Methodology口頭発表（一般）
- Metabolic Engineering 11, 2016年06月, 英語, Metabolic Engineering 11, 淡路市, 国際会議Engineering and Analyzing Streptomyces strain for the Recombinant Protein Production口頭発表（一般）
- Metabolic Engineering 11, 2016年06月, 英語, Metabolic Engineering 11, 淡路市, 国際会議Direct Fermentation of L-Lactic Acid from Starch By Genetically Engineered Aspergillus Oryzae口頭発表（一般）
- Metabolic Engineering 11, 2016年06月, 英語, Metabolic Engineering 11, 淡路市, 国際会議Development of Platform Yeast Strain Capable of Direct Fermentation of Raw Biomass to Ethanolポスター発表
- Metabolic Engineering 11, 2016年06月, 英語, Metabolic Engineering 11, 淡路市, 国際会議Caffeic Acid Production By Simultaneous Saccharification and Fermentation of Kraft Pulp Using Recombinant Escherichia coli口頭発表（一般）
- 化学工学会第81年会, 2016年03月, 日本語, 国内会議担癌マウスを用いたポリアクリル酸修飾過酸化チタンナノ粒子の生体内分布特性の調査口頭発表（一般）
- 日本農芸化学会2016年度大会, 2016年03月, 日本語, 国内会議紙パルプの同時糖化発酵によるカフェ酸の微生物生産口頭発表（一般）
- 日本農芸化学会2016年度大会, 2016年03月, 日本語, 国内会議マンナンバイオマスからのエタノール生産：βｰマンナナーゼとβｰマンノシダーゼを細胞表層に提示した出芽酵母の開発口頭発表（一般）
- 日本農芸化学会2016年度大会, 2016年03月, 日本語, 国内会議コリネ型細菌によるUV吸収性アミノ酸の生産口頭発表（一般）
- 日本農芸化学会2016年度大会, 2016年03月, 日本語, 国内会議アルコールの添加物が希硫酸前処理の効率に与える影響の解析口頭発表（一般）
- The 6th iBioK Asian Workshop, 2016年03月, 日本語, Kobe, 国際会議From mannan and lignocellulosic biomass to biochemicals: cell surface display and metabolic engineering in yeast Saccharomyces cerevisiae口頭発表（一般）
- 化学工学会第81年会, 2016年03月, 日本語, 国内会議AFM力計測を用いた細胞表層提示酵素の特異的検出とマッピング評価口頭発表（一般）
- 日本化学会第96春季年回, 2016年03月, 日本語, 国内会議AFM力学計測法を利用した細胞表層空間に提示した酵素検出口頭発表（一般）
- 化学工学会第81年会, 2016年03月, 日本語, 国内会議Affibody提示バイオナノカプセル/リポソーム融合粒子を用いたin vivo での抗腫瘍効果口頭発表（一般）
- 第11回バイオマス科学会議, 2016年01月, 日本語, 国際会議リグノセルロース系バイオマスの同時糖化発酵によるフェニル乳酸生産口頭発表（一般）
- 神戸大学若手フロンティア2015, 2015年12月, 日本語, 神戸大学, 国内会議稲わらのバイオリファイナリーに向けた特性の解析と多様性の評価ポスター発表
- 日本農芸化学会関西支部第492例会, 2015年12月, 日本語, 神戸大学, 国内会議バイオリファイナリー利用に向けた稲わらの希硫酸前処理後グルコース含量の自然変異口頭発表（一般）
- The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015), 2015年12月, 英語, 国際会議Specific drug delivery for target cancer tumor using affibody-displaying bionanocapsule/liposome complex口頭発表（一般）
- The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015), 2015年12月, 英語, 国際会議Live cell imaging and membrane protein mapping with atomic force microscope口頭発表（一般）
- Pacifichem 2015, 2015年12月, 英語, 国際会議Development of novel radiosensitizing cancer therapy:Combination of radio-therapy and titanium peroxide nanoparticle口頭発表（一般）
- Pacifichem 2015, 2015年12月, 英語, 国際会議Characterization for metal-oxide nano-perticles generating radicals with X-ray irrdiatio口頭発表（一般）
- 第15回糸状菌分子生物学コンファレンス, 2015年11月, 日本語, 国内会議コピー数多形とプロモーター強度を加味した黄麹菌でのマルチ遺伝子発現口頭発表（一般）
- 第15回糸状菌分子生物学コンファレンス, 2015年11月, 日本語, 国内会議L-乳酸生産黄麹菌の乳酸生産能に対する中央代謝経路改変の影響口頭発表（一般）
- 第67回日本生物工学会大会 国際シンポジウム, 2015年10月, 日本語, 国内会議代謝改変による赤色酵母を用いたアスタキサンチン生産性の向上口頭発表（一般）
- 第67回日本生物工学会大会 国際シンポジウム, 2015年10月, 日本語, 国内会議Streptoverticillium Cinnamoneum由来Phospholipase D プロモーター遺伝子発現機構の調査口頭発表（一般）
- 第67回日本生物工学会大会 国際シンポジウム, 2015年10月, 日本語, 国内会議L-乳酸生産株Aspergillus oryzaeの代謝改変によるLー乳酸生産能の改変口頭発表（一般）
- The 21th Symposium of Young Asian Biochemical Engineer's Communyty, 2015年10月, 英語, 国際会議Lignocellulosic biomass refinery using biocompatible ionic liquida口頭発表（一般）
- The 21th Symposium of Yong Asian Biochemical engineer's Communyty, 2015年10月, 英語, 国際会議Conversion of high phospholipid-containing oils to biodiesel using immobilizes Aspergillus oryzae whole cell biocatalyst ezpressing Fusarium heteroporum lipase口頭発表（一般）
- 化学工学会第47回秋季大会, 2015年09月, 日本語, 国内会議放射線治療と相乗効果を有するポリアクリル酸修飾過酸化チタンナノ粒子の作用機序解明口頭発表（一般）
- 化学工学会 第47回秋季大会, 2015年09月, 日本語, 化学工学会, 北海道大学, 国内会議超音波照射によるリグニン分解の反応速度解析口頭発表（一般）
- 化学工学会第47回秋季大会, 2015年09月, 日本語, 国内会議担癌マウスを用いたポリアクリル酸修飾過酸化チタンナノ粒子の生体内分布特性の調査口頭発表（一般）
- マテリアルサイエンス研究科セミナー, 2015年07月, 日本語, 国内会議バイオマスからの微生物によるバイオモノマー生産[招待有り]口頭発表（招待・特別）
- 6th AFOB Regional Symposium, 2015年05月, 英語, Asian Federation of Biotechnology, 国際会議Screening of ethanol and lactic acid-producing yeast from Indonesia culture collection口頭発表（一般）
- 6th AFOB Regional Symposium, 2015年05月, 英語, Asian Federation of Biotechnology, 国際会議Screening and characterization of Lignocellulose-hydrolytic from Indonesia Rare Actinomycetes and applications of these enzymes for sugar cane baggase saccharification to produce bioethanol口頭発表（一般）
- 日本農芸化学会2015年度大会, 2015年03月, 日本語, 日本農芸化学会, 岡山市, 国内会議膜濃縮したソルガム搾汁液からのエタノール発酵口頭発表（一般）
- 第17回化学工学会学生発表会, 2015年03月, 日本語, 化学工学会, 徳島市, 国内会議担癌マウスへのTiナノ粒子投与による生体内分布口頭発表（一般）
- 日本農芸化学会2015年度大会, 2015年03月, 日本語, 日本農芸化学会, 岡山市, 国内会議組換えコリネ型細菌による3-hydroxy-4-aminobenzoic acid発酵におけるソルガム搾汁液の促進作用口頭発表（一般）
- 日本農芸化学会2015年度大会, 2015年03月, 日本語, 日本農芸化学会, 岡山市, 国内会議実バイオマスを微生物変換するための新しい酵母プラットフォームの探索口頭発表（一般）
- 日本農芸化学会2015年度大会, 2015年03月, 日本語, 日本農芸化学会, 岡山市, 国内会議コリネ型細菌を用いたプロトカテク酸の生産口頭発表（一般）
- 日本化学会第95春季年会, 2015年03月, 日本語, 日本化学会, 船橋市, 国内会議AFM による細胞表層提示タンパク質の特異的検出およびマッピング口頭発表（一般）
- Active Enzyme Molecule 2014, 2014年12月, 英語, 酵素工学会, 富山市, 国内会議Construction of arming yeast displaying optimal ratio of cellulases for degradation of biomass pretreated with ionic liquidポスター発表
- 第14回糸状菌分子生物学コンファレンス, 2014年11月, 日本語, 糸状菌分子生物学研究会, 仙台市, 国内会議麹菌細胞工場を用いた L- および D-乳酸の生産ポスター発表
- 第15回極限環境生物学会年会, 2014年11月, 日本語, 極限環境生物学会, 沖縄市, 国内会議好熱性および好冷性酵素のドメインを持つキメラ酵素の構築ポスター発表
- 第14回糸状菌分子生物学コンファレンス, 2014年11月, 日本語, 糸状菌分子生物学研究会, 仙台市, 国内会議セルラーゼ遺伝子をマルチコピー導入した麹菌の遺伝子コピー数とセルラーゼ活性ポスター発表
- The 20th Symposium of Young Asian Biochemical Engineers' Communyty, 2014年11月, 英語, 生物工学会, Taiwan, China, 国際会議Microbial screening for biorefinery research口頭発表（一般）
- 第5回イオン液体討論会, 2014年10月, 日本語, イオン液体研究会, 横浜市, 国内会議イオン液体前処理後バガスに適したアーミング酵母の開発口頭発表（一般）
- 第66回日本生物工学会大会, 2014年09月, 日本語, 日本生物工学会, 札幌市, 国内会議放線菌を用いた糖を原料とするパラアミノ安息香酸の生産ポスター発表
- 第66回日本生物工学会大会, 2014年09月, 日本語, 日本生物工学会, 札幌市, 国内会議麹菌を用いたデンプンからのD- 乳酸の同時糖化発酵ポスター発表
- 第66回日本生物工学会大会, 2014年09月, 日本語, 日本生物工学会, 札幌市, 国内会議稲わら前処理液の膜プロセスによるエタノール発酵高効率化ポスター発表
- 第66回日本生物工学会大会, 2014年09月, 日本語, 日本生物工学会, 札幌市, 国内会議バイオマス糖化液由来成分が大腸菌のフェニル乳酸発酵に与える影響ポスター発表
- 第66回日本生物工学会大会, 2014年09月, 日本語, 日本生物工学会, 札幌市, 国内会議pH 応答性ペプチドGALA を表層提示したバイオナノカプセルのエンドソーム脱出ポスター発表
- 生物工学若手研究者の集い夏のセミナー, 2014年07月, 日本語, 生物工学会, 神戸市, 国内会議ｐH応答性膜融合ペプチドGALAを表層提示したバイオナノカプセルのエンドソーム脱出ポスター発表
- 生物工学若手研究者の集い夏のセミナー, 2014年07月, 日本語, 生物工学会, 神戸市, 国内会議放射線がん治療との併用に向けたか酸化チタンナノ粒子の物性評価ポスター発表
- 生物工学若手研究者の集い夏のセミナー, 2014年07月, 日本語, 生物工学会, 神戸市, 国内会議上皮成長因子受容体を特異的に認識するAffibody提示バイオナノカプセルの開発ポスター発表
- 生物工学若手研究者の集い夏のセミナー, 2014年07月, 日本語, 生物工学会, 神戸市, 国内会議リグノセルロース系バイオマスからのバイオ芳香族化合物の生産ポスター発表
- 第60回高分子研究発表会（神戸）, 2014年07月, 日本語, 高分子学会, 神戸市, 国内会議カーボンナノチューブの表面改質を目指した重合性界面活性剤の開発ポスター発表
- 生物工学若手研究者の集い夏のセミナー, 2014年07月, 日本語, 生物工学会, 神戸市, 国内会議AFMを用いた表層提示酵母へのアプローチ及び表層提示酵素の検出ポスター発表
- 第63回高分子学会年次大会, 2014年05月, 日本語, 高分子学会, 名古屋市, 国内会議カーボンナノチューブの可溶化を目指した重合性界面活性剤の開発ポスター発表
- 日本農芸化学会2014年度大会, 2014年03月, 日本語, 東京, 国内会議放線菌による 3,4-AHBA 合成とキシロース代謝口頭発表（一般）
- 日本農芸化学会2014年度大会, 2014年03月, 日本語, 日本農芸化学会, 川崎市, 国内会議同時糖化発酵による希硫酸前処理バイオマスからのフェニル乳酸生産口頭発表（一般）
- 化学工学会第79年会, 2014年03月, 日本語, 化学工学会, 岐阜市, 国内会議新しい放射線治療をめざした過酸化チタンナノ粒子の物性研究ポスター発表
- 第55回日本植物生理学会年会, 2014年03月, 日本語, 富山, 国内会議バイオマス評価法の開発ポスター発表
- 第16回化学工学会学生発表会(堺大会), 2014年03月, 日本語, 化学工学会, 大阪市, 国内会議タンパク質提示酵母の表層における親和力解析口頭発表（一般）
- 第8回日本ゲノム微生物学会年会, 2014年03月, 日本語, 日本ゲノム微生物学会, 世田谷区, 国内会議タンパク高生産放線菌のゲノム変異解析と因子探索ポスター発表
- 日本農芸化学会2014年度大会, 2014年03月, 日本語, 日本農芸化学会, 川崎市, 国内会議セルロース系バイオマスからの物質生産を可能にしたスーパー麹菌の開発口頭発表（一般）
- 日本農芸化学会2014年度大会, 2014年03月, 日本語, 日本農芸化学会, 川崎市, 国内会議コリネ型細菌を用いた糖を原料とするGABAの生産口頭発表（一般）
- 化学工学会第79年会, 2014年03月, 日本語, 化学工学会, 岐阜市, 国内会議イオン液体を用いたリグノセルロースのトータルリファイナリー口頭発表（一般）
- 化学工学会第79年会, 2014年03月, 日本語, 化学工学会, 岐阜市, 国内会議イオン液体と酸を用いた新規バイオマス前処理法の開発と応用ポスター発表
- 第4回イオン液体研究会, 2013年11月, 日本語, イオン液体研究会, 横浜市, 国内会議低細胞毒性イオン液体により前処理した木質系バイオマスからの同時糖化発酵ポスター発表
- 第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議放線菌による芳香族化合物ホモゲンチジン酸の生産ポスター発表
- 第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議同時糖化発酵によるセルロースからのフェニル乳酸生産ポスター発表
- 第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議低細胞毒性イオン液体により前処理した木質系バイオマスからの同時糖化発酵ポスター発表
- 第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議低コストイオン液体によるエタノール製造プロセスの提案ポスター発表
- 第62回高分子討論会, 2013年09月, 日本語, 高分子学会, 金沢市, 国内会議新しい重合性界面活性剤を用いた難溶性ナノ材料可溶化技術の開発口頭発表（一般）
- 第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議上皮成長因子受容体を特異的に認識するAffibody提示バイオナノカプセルの開発ポスター発表
- 日本農芸化学会関西;中四国;西日本支部合同大会（第481回講演会）, 2013年09月, 日本語, 日本農芸化学会, 広島市, 国内会議酸触媒イオン液体前処理を用いた新規バイオプロセス構築口頭発表（一般）
- 日本農芸化学会関西;中四国;西日本支部合同大会（第481回講演会）, 2013年09月, 日本語, 日本農芸化学会, 広島市, 国内会議麹菌Aspergillus oryzae によるL-乳酸の生産口頭発表（一般）
- 平成２５年度繊維学会秋季研究発表会, 2013年09月, 日本語, 名古屋市, 国内会議各種イネ科植物から作製したセルロースナノファイバーシートの物性評価ポスター発表
- 第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議稲わら希硫酸処理液のナノフィルトレーションによる糖濃縮ポスター発表
- 第31回日本植物細胞分子生物学会大会;シンポジウム, 2013年09月, 日本語, 日本植物細胞分子生物学会, 札幌市, 国内会議易糖化性を示すイネの探索口頭発表（一般）
- 化学工学会第45回秋季大会, 2013年09月, 日本語, 化学工学会, 岡山市, 国内会議バイオリファイナリーを目指したイオン液体の活用ポスター発表
- 第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議バイオマス分解性向上を目的とする酸導入イオン液体を用いた新規前処理法開発ポスター発表
- 第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議ナノ粒子とX線照射を併用した深部ガンの非侵襲的治療法の開発ポスター発表
- 第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議コリネ型細菌を用いたプロトカテク酸の生産ポスター発表
- セルロース学会第20回年次大会, 2013年07月, 日本語, 京都市, 国内会議各種イネ科植物から作製したセルロースナノファイバーシートの物性ポスター発表
- 第2回JACI/GSC 講演会, 2013年06月, 日本語, 新化学技術推進協会, 大阪市, 国内会議植物源を異にするセルロースナノファイバーシートの構造と物性ポスター発表
- 第2回JACI/GSC 講演会, 2013年06月, 日本語, 新化学技術推進協会, 大阪市, 国内会議イオン液体を用いたバイオマスの前処理と同時糖化発酵ポスター発表
- 化学工学会 第78年会, 2013年03月, 日本語, 化学工学会, 大阪大学, 国内会議重合性界面活性剤による難溶性材料可溶化技術の開発口頭発表（一般）
- 日本化学会第93春季年会, 2013年03月, 日本語, 日本化学会, 草津市, 国内会議原子間力顕微鏡を用いた細胞表層解析法および細胞応答解析法の開発口頭発表（一般）
- 化学工学会第78年会, 2013年03月, 日本語, 化学工学会, 豊中市, 国内会議Ethanol production from ionic liquid pretreated biomass by recombinant yeast口頭発表（一般）
- 第３回イオン液体討論会, 2012年12月, 日本語, イオン液体研究会, 那覇市, 国内会議コリン系イオン液体と超音波を組み合わせた木質系バイオマス前処理口頭発表（一般）
- 第３回イオン液体討論会, 2012年12月, 日本語, イオン液体研究会, 那覇市, 国内会議イオン液体耐性セルラーゼの単離と酵素特性解析口頭発表（一般）
- International Joint Symposium on Single-Cell Analysis (The 6th International Workshop on Approaches to Single-Cell Analysis & The 8th International Forum on Post-Genome Technologies), 2012年11月, 英語, The Society for Single-Cell Surveyor, 京都市, 国際会議Interaction survey between GPCR and ligand on the surface of yeast by AFMポスター発表
- 第64回日本生物工学会大会, 2012年10月, 日本語, 日本生物工学会, 神戸市, 国内会議放線菌バイオマス分解酵素ライブラリの構築及び新規高分泌シグナルの探索口頭発表（一般）
- 酵素工学研究会第６８回講演会, 2012年10月, 日本語, 酵素工学研究会, 文京区, 国内会議ピリジニウム系イオン液体による結晶性セルロースのin situ 酵素糖化ポスター発表
- 第64回日本生物工学会大会, 2012年10月, 日本語, 日本生物工学会, 神戸市, 国内会議エンドグルカナーゼ分泌生産型放線菌によるセルロースからの安息香酸生産口頭発表（一般）
- 酵素工学研究会第６８回講演会, 2012年10月, 日本語, 酵素工学研究会, 文京区, 国内会議エンドグルカナーゼ分泌生産型放線菌によるセルロースからの安息香酸生産ポスター発表
- 第64回日本生物工学会大会, 2012年10月, 日本語, 日本生物工学会, 神戸市, 国内会議イオン液体耐性セルラーゼの探索と機能解析口頭発表（一般）
- 酵素工学研究会第６８回講演会, 2012年10月, 日本語, 酵素工学研究会, 文京区, 国内会議イオン液体耐性セルラーゼの単離と酵素特性解析ポスター発表
- 第64回日本生物工学会大会, 2012年10月, 日本語, 日本生物工学会, 神戸市, 国内会議イオン液体前処理による木質系バイオマスからのエタノール生産口頭発表（一般）
- 第64回日本生物工学会大会, 2012年10月, 日本語, 日本生物工学会, 神戸市, 国内会議イオン液体前処理とキシロース資化性酵母株を用いたバイオマス糖化発酵プロセスの効率化口頭発表（一般）
- The 18th Symposium of Young Asian Biochemical Engineers' Community (YABEC2012), 2012年10月, 英語, Society for Biological Engineering, Tokushima, Japan, 国際会議Genetic engineering of valine biosynthesis for isobutanol production in Saccharomyces cerevisiae口頭発表（一般）
- The 18th Symposium of Young Asian Biochemical Engineers' Communyty, 2012年10月, 英語, ㈳日本生物工学会, 徳島市, 国際会議Direct ethanol production from lignocellulosic biomass pretreated with ionic liquid from cellulase-displaying yeastポスター発表
- 第64回日本生物工学会大会, 2012年10月, 日本語, 日本生物工学会, 神戸市, 国内会議Cell SELEX 法により選抜したヒト肝臓由来がん細胞に対するDNA アプタマーの評価口頭発表（一般）
- The 15th International Biotechnology Symposium (IBS 2012), 2012年09月, 英語, The Korean Society for Biotechnology and Bioengineering, Deague, Korea, 国際会議Complex carriers of affibody-displaying bio-nanocapsule and composition-varied liposomes for HER2-expressing breast cancer cell-specific protein delivery口頭発表（一般）
- Metabolic EngineeringⅨ, 2012年06月, 英語, Engineering Conferences International, Biarritz, France, 国際会議Genetic engineering to enhance the Ehrlich pathway and alter carbon flux for increased isobutanol production by Saccharomyces cerevisiae口頭発表（一般）
- 21世紀を拓くバイオテクノロジーシンポジウム, 2012年06月, 日本語, YABEC2012実行委員会, 徳島市, 国内会議AFMを用いた新規核酸分子の探索口頭発表（一般）
- 第72回分析化学討論会, 2012年05月, 日本語, 日本分生化学会, 鹿児島市, 国内会議ラジカルを発生する金属酸化物粒子に対するXAFSによる非破壊状態分析口頭発表（一般）
- The 12th Japan-China-Korea Joint Symposium on Enzyme Engineering, 2012年05月, 英語, 酵素工学研究会, 金沢市, 国際会議DNA aptamer screening and evaluation by AFM口頭発表（一般）
- 化学工学会第77年会, 2012年03月, 日本語, 化学工学会, 東京, 国内会議動的周期操作を用いたエタノール連続発酵プロセスにおける分岐現象口頭発表（一般）
- 日本農芸化学会2012年度大会, 2012年03月, 日本語, 日本農芸化学会, 京都市, 国内会議麹菌Aspergillus oryzaeを用いたラクダ科動物由来一本鎖抗体可変部位VHH分泌生産系の構築口頭発表（一般）
- 日本化学会第92春季年会, 2012年03月, 日本語, 日本化学会, 横浜市, 国内会議原子間力顕微鏡を用いた細胞表層におけるレセプター・リガンド間相互作用測定系の構築口頭発表（一般）
- 日本農芸化学会2012年度大会, 2012年03月, 日本語, 日本農芸化学会, 京都市, 国内会議海洋性超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの構造安定性口頭発表（一般）
- 日本農芸化学会2012年度大会, 2012年03月, 日本語, 日本農芸化学会, 京都市, 国内会議海洋性超好熱菌Thermotoga neapolitana由来β-1,3-キシラナーゼの耐熱性を導く要因の解析口頭発表（一般）
- 化学工学会第77年会, 2012年03月, 日本語, 化学工学会, 新宿区, 国内会議ピリジニウム系イオン液体による結晶性セルロースの前処理・酵 素糖化・高速発酵(その2)口頭発表（一般）
- 化学工学会第77年会, 2012年03月, 日本語, 化学工学会, 新宿区, 国内会議ピリジニウム系イオン液体による結晶性セルロースの前処理・酵素糖化・高速発酵(その1)口頭発表（一般）
- 日本化学会第92春季年会, 2012年03月, 日本語, 日本化学会, 横浜市, 国内会議ソマトスタチンに対する新規DNA アプタマーの選抜およびセンシングへの応用口頭発表（一般）
- 化学工学会第77年会, 2012年03月, 日本語, 化学工学会, 新宿区, 国内会議セルロースからの高効率一段階エタノール発酵を可能にする高機能酵母の創製口頭発表（一般）
- 日本農芸化学会2012年度大会, 2012年03月, 日本語, 日本農芸化学会, 京都市, 国内会議コリネ型細菌を用いたプトレシンの生産口頭発表（一般）
- 日本農芸化学会2012年度大会, 2012年03月, 日本語, 日本農芸化学会, 京都市, 国内会議エンドグルカナーゼ分泌生産型放線菌を用いたセルロースからの有用タンパク質生産口頭発表（一般）
- 化学工学会第77年会, 2012年03月, 日本語, 化学工学会, 新宿区, 国内会議エンドグルカナーゼ分泌生産型放線菌によるセルロースからの安息香酸生産口頭発表（一般）
- 第2回イオン液体討論会, 2011年12月, 日本語, イオン液体研究会, 京都市, 国内会議リグノセルロース・リファイナリーにおけるイオン液体の役割口頭発表（一般）
- 第2回イオン液体討論会, 2011年12月, 日本語, イオン液体研究会, 京都市, 国内会議イオン液体耐性セルラーゼの探索と機能評価口頭発表（一般）
- 第2回イオン液体討論会, 2011年12月, 日本語, イオン液体研究会, 京都市, 国内会議イオン液体前処理バイオマスを用いたエタノール同時糖化発酵口頭発表（一般）
- 第2回イオン液体討論会, 2011年12月, 日本語, イオン液体研究会, 京都市, 国内会議イオン液体前処理バイオマスの構造解析およびエタノール発酵口頭発表（一般）
- 第2回イオン液体討論会, 2011年12月, 日本語, イオン液体研究会, 京都市, 国内会議イオン液体共存下における酵母のキシロース発酵能評価口頭発表（一般）
- 第34回日本分子生物学会年会, 2011年12月, 英語, 日本分子生物学会, 横浜市, 国内会議Amplification and smoothing of signal activation from human G-protein-coupled receptor for enriching agonist stimulation in yeastポスター発表
- 極限環境生物学会2011年度(第12回)年会, 2011年11月, 日本語, 極限環境生物学会, 長崎市, 国内会議超好熱性細菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの結晶構造解析ポスター発表
- 極限環境生物学会2011年度(第12回)年会, 2011年11月, 日本語, 極限環境生物学会, 長崎市, 国内会議超好熱性細菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの安定性解析ポスター発表
- 第11回糸状菌分子生物学コンファレンス(2011), 2011年11月, 日本語, 糸状菌分子生物学研究会, 文京区, 国内会議組換え麹菌生産セルラーゼを用いた広葉樹クラフトパルプの酵素糖化ポスター発表
- 第11回糸状菌分子生物学コンファレンス(2011), 2011年11月, 日本語, 糸状菌分子生物学研究会, 文京区, 国内会議ラクダ由来一本鎖抗体可変部位 VHH の麹菌 Aspergillus oryzae による分泌生産ポスター発表
- 化学工学会第43回秋季大会, 2011年09月, 日本語, 化学工学会, 名古屋市, 国内会議放線菌が有する炭素源資化能力を生かしたタンパク質の大量分泌生産ポスター発表
- 第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議組換え酵母による高効率グルタチオン生産口頭発表（一般）
- 第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議生体分子融合ナノ粒子の生物化学工学領域への高度応用に関する研究[招待有り]口頭発表（招待・特別）
- 第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議麹菌Aspergillus oryzae による超好熱菌Thermotoga neapolitana 由来耐熱性β-1,4- マンナナーゼの生産口頭発表（一般）
- 第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議酵母によるバイオマスからのグルタチオンの直接発酵口頭発表（一般）
- 第29回日本植物細胞分子生物学会大会, 2011年09月, 日本語, 日本植物細胞分子生物学会, 福岡市, 国内会議稲わらバイオマス形質の系統間比較口頭発表（一般）
- 化学工学会第43回秋季大会, 2011年09月, 日本語, 化学工学会, 名古屋市, 国内会議βグルコシダーゼを表層提示したセロオリゴ糖資化性大腸菌の創製ポスター発表
- 第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議セルラーゼ分泌酵母によるイオン液体処理バイオマスからのエタノール生産口頭発表（一般）
- 化学工学会第43回秋季大会, 2011年09月, 日本語, 化学工学会, 名古屋市, 国内会議セルラーゼ発現最適化二倍体酵母による木質系バイオマスからのエタノール発酵ポスター発表
- 第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議イオン液体処理が結晶性セルロース酵素糖化に与える影響口頭発表（一般）
- 第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議イオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発口頭発表（一般）
- 化学工学会第43回秋季大会, 2011年09月, 日本語, 化学工学会, 名古屋市, 国内会議Corynebacterium glutamicumを用いた高効率なGABA生産技術の開発ポスター発表
- 第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議BGL 表層提示大腸菌によるセロビオースを基質としたIPA 生産口頭発表（一般）
- 第63回日本生物工学会大会, 2011年09月, 日本語, 日本生物工学会, 小金井市, 国内会議Affibody 提示Bio-nanocapsule を用いたER2 発現癌細胞へのタンパク質送達システム口頭発表（一般）
- Defence, Science & Research (DSR 2011) Conference, 2011年08月, 英語, Committee of DSR2011, Singapore, 国際会議Aromatic chemicals production using phenylalnine ammonia lyase expressing Streptomyces lividans口頭発表（一般）
- 第27回日本DDS学会学術集会, 2011年06月, 日本語, 日本DDS学会, 文京区, 国内会議バイオナノカプセルを用いた細胞特異的ドラッグデリバリーシステム口頭発表（一般）
- International Conference on Materials for Asdvanced Technologies 2011, 2011年06月, 英語, Materials Research Society Singapore, Singapore, 国際会議Key Monomers Production from Biomass Toward Bio-based Polymers Using口頭発表（一般）
- 第60回高分子学会年次大会, 2011年05月, 日本語, 高分子学会, 大阪市, 国内会議イオン液佒へのセルロースの溶解とバイオエタノール生産口頭発表（一般）
- 33rd Symposium on Biotechnology for Fuels and Chemicals, 2011年05月, 英語, Society for industrial Microbiology, Seattle, USA, 国際会議Homo D-lactic acid fermentation from pentose and xylan by metabolically engineered Lactobacillus plantarumポスター発表
- 2011 International Meeting of the Microbiological Society of Korea, 2011年05月, 英語, The Microbiological Society of Korea, Gwangju, Korea, 国際会議Effective Screening of Optimized Diploid Saccharomyces cerevisiae Strain for Direct Ethanol Production from Cellulosic Materials by Displaying Ratio Adjustment of Cellulolytic Enzymes口頭発表（一般）
- 33rd Symposium on Biotechnology for Fuels and Chemicals, 2011年05月, 英語, Society for industrial Microbiology, Seattle, USA, 国際会議Cinnamic acid production from various carbon sources using phenylalnine ammonia lyase expressing Streptomyces lividansポスター発表
- E-MRS ICAM IUMRS 2011 Spring Meeting, 2011年05月, 英語, European Materials Research Society, Nice, France, 国際会議Characterization of Titanium oxide nano-particle suspension by XAFS technique口頭発表（一般）
- 化学工学会第76年会, 2011年03月, 日本語, 日本化学会, 東京都 小金井市, 国内会議放線菌を用いたあらゆる炭素源からのケイ皮酸生産口頭発表（一般）
- 日本農芸化学会2011年度大会, 2011年03月, 日本語, 日本農芸化学会, 京都市, 国内会議放線菌によるトランスグルタミナーゼの大量分泌生産に向けた生産条件の検討口頭発表（一般）
- 化学工学会第15回学生発表会, 2011年03月, 日本語, (社)化学工学会, 神戸市, 国内会議放線菌によるトランスグルタミナーゼの生産口頭発表（一般）
- 化学工学会第15回学生発表会, 2011年03月, 日本語, (社)化学工学会, 神戸市, 国内会議放線菌における表層提示技術に関する研究口頭発表（一般）
- 化学工学会第76年会, 2011年03月, 日本語, 日本化学会, 東京都 小金井市, 国内会議長期エタノール発酵に向けたアミラーゼ表層提示発現酵母株の創製口頭発表（一般）
- 化学工学会第76年会, 2011年03月, 日本語, 日本化学会, 東京都 小金井市, 国内会議代謝工学を活用したイソブタノール生産酵母の創製口頭発表（一般）
- 日本農芸化学会2011年度大会, 2011年03月, 日本語, 日本農芸化学会, 京都市, 国内会議組換え酵母による高効率グルタチオン生産口頭発表（一般）
- 化学工学会第15回学生発表会, 2011年03月, 日本語, (社)化学工学会, 神戸市, 国内会議酵母を用いたバイオマス資源からのグルタチオン生産技術の開発口頭発表（一般）
- 化学工学会第76年会, 2011年03月, 日本語, 日本化学会, 東京都 小金井市, 国内会議酵母を用いたバイオマスからのグルタチオンの生産口頭発表（一般）
- 日本化学会第91春季年会, 2011年03月, 日本語, 化学工学会, 横浜市, 国内会議酵素活性を制御するDNAアプタマーの探索口頭発表（一般）
- 日本化学会第91春季年会, 2011年03月, 日本語, 化学工学会, 横浜市, 国内会議原子間力顕微鏡を用いた機能性核酸分子の選抜口頭発表（一般）
- 化学工学会第15回学生発表会, 2011年03月, 日本語, (社)化学工学会, 神戸市, 国内会議原子間力顕微鏡を用いた機能性核酸分子の選抜口頭発表（一般）
- 日本化学会第91春季年会, 2011年03月, 日本語, 化学工学会, 横浜市, 国内会議原子間力顕微鏡を用いたアミノ酸に対する機能性核酸分子・アプタマーの新規選抜法の開発口頭発表（一般）
- 化学工学会第76年会, 2011年03月, 日本語, 日本化学会, 東京都 小金井市, 国内会議過酸化チタンナノ粒子及び低線量X線照射を併用した深部ガンの非侵襲的治療法の開発口頭発表（一般）
- 化学工学会第15回学生発表会, 2011年03月, 日本語, (社)化学工学会, 神戸市, 国内会議過酸化チタンナノ粒子及び低線量X線照射を併用した深部ガンの非侵襲的治療法の開発口頭発表（一般）
- 日本農芸化学会2011年度大会, 2011年03月, 日本語, 日本農芸化学会, 京都市, 国内会議βグルコシダーゼ表層提示によるセロビオース資化性大腸菌の創製口頭発表（一般）
- 化学工学会第76年会, 2011年03月, 日本語, 日本化学会, 東京都 小金井市, 国内会議βグルコシダーゼを表層提示したセロビオース資化性大腸菌の創製口頭発表（一般）
- 化学工学会第15回学生発表会, 2011年03月, 日本語, (社)化学工学会, 神戸市, 国内会議ランダム変異導入による高活性エンドグルカナーゼの創製口頭発表（一般）
- 化学工学会第15回学生発表会, 2011年03月, 日本語, (社)化学工学会, 神戸市, 国内会議トランスポゾンタギング法による好塩性細菌Halomonas elongata OUT30018株の浸透圧ストレス応答性遺伝子の探索口頭発表（一般）
- 日本化学会第91春季年会, 2011年03月, 日本語, 化学工学会, 横浜市, 国内会議ソマトスタチンに対する新規DNAアプタマーの選抜口頭発表（一般）
- 日本農芸化学会2011年度大会, 2011年03月, 日本語, 日本農芸化学会, 京都市, 国内会議コリネ型細菌を用いたGABAの生産口頭発表（一般）
- 日本化学会第91春季年会, 2011年03月, 日本語, 化学工学会, 横浜市, 国内会議イオン液体共存下でのセルロース系バイオマスからの直接エタノール発酵口頭発表（一般）
- 日本農芸化学会2011年度大会, 2011年03月, 日本語, 日本農芸化学会, 京都市, 国内会議イオン液体を利用したバイオマスの前処理・糖化・発酵技術口頭発表（一般）
- 化学工学会第76年会, 2011年03月, 日本語, 日本化学会, 東京都 小金井市, 国内会議イオン液体をベースとしたセルロース系バイオマスの前処理とエタノール発酵プロセス口頭発表（一般）
- 化学工学会第76年会, 2011年03月, 日本語, 日本化学会, 東京都 小金井市, 国内会議イオン液体によるバイオマスの前処理・糖化・発酵技術の開発口頭発表（一般）
- 日本農芸化学会2011年度大会, 2011年03月, 日本語, 日本農芸化学会, 京都市, 国内会議Streptomyces griseocarneusスフィンゴミエリナーゼCの速度論的解析とMg2+による活性増強メカニズムの解明口頭発表（一般）
- The 5th International Workshop on Approaches to Single-Cell Analysis, 2011年03月, 英語, Tokyo, Japan, 国際会議Single-cell analysis revealed correlation between yeast GPCR signaling and plasmid retentionポスター発表
- 化学工学会第15回学生発表会, 2011年03月, 日本語, (社)化学工学会, 神戸市, 国内会議Corynebacterium glutamicumを用いたGABAの高効率生産技術の開発口頭発表（一般）
- 日本化学会第91春季年会, 2011年03月, 日本語, 化学工学会, 横浜市, 国内会議AFMを用いた生細胞表層のレセプター・リガンド間相互作用解析と細胞応答の観察口頭発表（一般）
- 芦屋川カレッジ, 2011年02月, 日本語, 芦屋市公民館, 芦屋市, 国内会議植物から燃料を作る口頭発表（招待・特別）
- 第23回CES21講演会, 2011年02月, 日本語, 日本分子生物学会, 大阪市, 国内会議金属酸化ナノ粒子と物理的励起を併用したラジカル発生システムを用いた細胞損傷システムの構築口頭発表（招待・特別）
- The 2010 International Chemical Congress of Pacific Basin Societies (Pacifichem 2010), 2010年12月, 英語, Honolulu, Hawaii, USA, 国際会議High-resolution, quantitative signalling assay for G-protein-coupled receptors by flow cytometry口頭発表（一般）
- 第10回 糸状菌分子生物学コンファレンス, 2010年11月, 日本語, 糸状菌分子生物学研究会, 東広島市, 国内会議麹菌A. oryzaeによる海洋細菌由来β-1,3-キシラナーゼの生産ポスター発表
- 第10回 糸状菌分子生物学コンファレンス, 2010年11月, 日本語, 糸状菌分子生物学研究会, 東広島市, 国内会議麹菌A. oryzaeにより生産した海洋細菌由来β-1,3-キシラナーゼの酵素学的諸性質ポスター発表
- The 16th Symposium of Young Asian Biochemical Engineers' Communyty, 2010年11月, 英語, (社)化学工学会, 台湾桃園県, 国際会議Selection of DNA aptamers using Atomic Force Microscopy口頭発表（一般）
- International Conference on Sustainable Biomass (ICSB) 2010, 2010年11月, 英語, 不明, Tamsui, Taiwan, 国際会議Ionic liquid Pretreatment for Direct Ethanol Production from Cellulosic Material by Cellulase-displaying Arming Yeast口頭発表（一般）
- 第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議放線菌を用いた様々なタンパクの大量分泌系の構築ポスター発表
- 第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議超好熱菌Thermotoga neapolitana 由来耐熱性β -1,3- キシラナーゼの機能解析ポスター発表
- 第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議麹菌による超好熱菌Thermotoga neapolitana 由来耐熱性β -1,3- キシラナーゼの高分泌生産ポスター発表
- 第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議過酸化チタンナノ粒子及び低線量X 線照射を併用した深部ガンの非侵襲的治療法の開発ポスター発表
- 第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議セルラーゼ発現バランス最適化酵母を用いたセルロースからのエタノール発酵ポスター発表
- 第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議イオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発(その4)ポスター発表
- 第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議イオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発(その3)ポスター発表
- 第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議イオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発(その2)ポスター発表
- 第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議Cell SELEX 法によるヒト肝臓由来がん細胞に選択的に結合するDNA アプタマーの選抜ポスター発表
- 第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議Affibody 提示ナノカプセルを用いたHER2 発現癌細胞特異的な薬物送達ポスター発表
- 第62回日本生物工学会大会, 2010年10月, 日本語, (社)日本生物工学会, 宮崎市, 国内会議1細胞アッセイ技術に基づくGPCR 解析、アゴニスト探索法の開発口頭発表（一般）
- 化学工学会第42回秋季大会, 2010年09月, 日本語, (社)化学工学会, 京都市, 国内会議力学的指標による生細胞表面におけるリガンド‐受容体の相互作用解析ポスター発表
- 2010年度日本放線菌学会大会, 2010年09月, 日本語, 日本放線菌学会, 東京都 江戸川区, 国内会議放線菌を用いた様々なタンパクの大量分泌系の構築口頭発表（一般）
- 日本応用糖質科学会 平成22年度大会(第59回), 2010年09月, 日本語, 日本応用糖質科学会, 静岡市, 国内会議超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの発現および諸性質口頭発表（一般）
- 日本応用糖質科学会 平成22年度大会(第59回), 2010年09月, 日本語, 日本応用糖質科学会, 静岡市, 国内会議麹菌(Aspergillus oryzae)を宿主とした超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの分泌高生産口頭発表（一般）
- 化学工学会第42回秋季大会, 2010年09月, 日本語, (社)化学工学会, 京都市, 国内会議高効率バイオディーゼル生産を目的としたWhole-cell biocatalystによる新規リパーゼ複合系の開発ポスター発表
- 化学工学会第42回秋季大会, 2010年09月, 日本語, (社)化学工学会, 京都市, 国内会議酵母によるエタノール発酵に及ぼす撹拌剪断効果ポスター発表
- 化学工学会第42回秋季大会, 2010年09月, 日本語, (社)化学工学会, 京都市, 国内会議機能性酵母によるバイオエタノール生産プロセスの強化ポスター発表
- 化学工学会第42回秋季大会, 2010年09月, 日本語, (社)化学工学会, 京都市, 国内会議affibody提示バイオナノカプセルを用いたタンパク質デリバリーシステムポスター発表
- RENEWABLE ENERGY 2010, 2010年06月, 英語, International Solar Energy Society, 横浜市, 国際会議Construction of consolidated bioprocesses for production of bio-fuels and chemicals by using cell surface engineered microbial cells口頭発表（一般）
- Industrial Biotechnology International Conference 2010, 2010年04月, 英語, The Italian Association of Chemical Engineering BIOTECH WORKING GROUP, Milano, Italy, 国際会議Intensification of Bioethanol Fermentation by Recombinant Yeast with Xylose Isomerase Pathway口頭発表（一般）
- 化学工学会第75年会, 2010年03月, 日本語, (社)化学工学会, 鹿児島市, 国内会議放線菌を用いた様々なタンパクの大量分泌系の構築口頭発表（一般）
- 化学工学会第75年会, 2010年03月, 日本語, (社)化学工学会, 鹿児島市, 国内会議非侵襲性がん治療に向けた高機能性二酸化チタンナノ粒子の開発口頭発表（一般）
- 化学工学会第75年会, 2010年03月, 日本語, (社)化学工学会, 鹿児島市, 国内会議生デンプンからの効率的な繰返し発酵を達成するアミラーゼ発現新規酵母株の創製口頭発表（一般）
- 日本農芸化学会2010年度大会, 2010年03月, 日本語, (社)日本農芸化学会, 東京都目黒区, 国内会議細胞表層工学技術を用いたヘミセルロース系バイオマスからのエタノール生産口頭発表（一般）
- 日本農芸化学会2010年度大会, 2010年03月, 日本語, (社)日本農芸化学会, 東京都目黒区, 国内会議酵母を用いた生デンプンからの高効率な機能性ペプチド生産口頭発表（一般）
- 日本化学会第90春季年会, 2010年03月, 日本語, 日本化学会, 東大阪市, 国内会議原子間力顕微鏡を用いた細胞表層におけるリガンド・レセプター間相互作用測定系の構築口頭発表（一般）
- 日本化学会第90春季年会, 2010年03月, 日本語, 日本化学会, 東大阪市, 国内会議原子間力顕微鏡を用いた機能性核酸分子・アプタマーの新規選抜法の開発口頭発表（一般）
- 日本化学会第90春季年会, 2010年03月, 日本語, 日本化学会, 東大阪市, 国内会議機能性酵母によるイオン液体前処理セルロースからのバイオエタノール生産口頭発表（一般）
- 日本農芸化学会2010年度大会, 2010年03月, 日本語, (社)日本農芸化学会, 東京都目黒区, 国内会議バイオマスからの燃料・化学品生産を目指した細胞工場の創製口頭発表（一般）
- 化学工学会第75年会, 2010年03月, 日本語, (社)化学工学会, 鹿児島市, 国内会議セルラーゼ表層提示酵母を用いたイオン液体前処理セルロースからの直接エタノール発酵口頭発表（一般）
- 日本農芸化学会2010年度大会, 2010年03月, 日本語, (社)日本農芸化学会, 東京都目黒区, 国内会議カクテルδインテグレーション法によるセルラーゼ発現バランス最適化酵母の創製口頭発表（一般）
- 日本化学会第90春季年会, 2010年03月, 日本語, 日本化学会, 東大阪市, 国内会議DNAアプタマーを用いた酵素活性制御系の構築口頭発表（一般）
- 日本化学会第90春季年会, 2010年03月, 日本語, 日本化学会, 東大阪市, 国内会議AFMを用いたアプタマーによるタンパク質の検出・定量方法口頭発表（一般）
- 第32回日本分子生物学会年会, 2009年12月, 日本語, 日本分子生物学会, 横浜市, 国内会議セルラーゼ提示超分子複合体提示酵母によるバイオマスの効率的分解口頭発表（招待・特別）
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議Screening of DNA aptamers that recognize amino acid by AFM-SELEX strategyポスター発表
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議Protein production for Bio-refinery strategyポスター発表
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議Production of biodiesel fuel in ionic liquids catalyzed by whole-cell biocatalysts口頭発表（一般）
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議New SELEX strategy for screening of DNA aptamer by AFM口頭発表（一般）
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議Investigation of the interaction between GPCR and ligand by AFM equipped with bio-molecule modified cantileverポスター発表
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議Engineering of endoglucanase expression system for Corynebacterium glutamicumポスター発表
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議Efficient repeated batch fermentation from raw starch by novel yeast expressing transporter gene and amylase genesポスター発表
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議Efficient homo D-lactic acid production from xyloseポスター発表
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議Efficient ethanol production from xylose by mated diploid Saccharomyces cerevisiaeポスター発表
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議Efficient D-lactic acid production from raw starchポスター発表
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議Efficient and practical ethanol production from high yield rice by amylase expressing Saccharomyces cerevisiaeポスター発表
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議Direct fermentation of cellulosic materials to ethanol using yeast strains codisplaying three types of cellulolytic enzymeポスター発表
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議Development of novel cell-surface display system of Aspergillus oryzae using chitin binding domainポスター発表
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議Bioethaol fermentation from mixed sugar by the recombinant yeast with xyloseisomerase pathwayポスター発表
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議Bioethanol production from mixed sugars using sugar uptake ability enhanced yeast strain by overexpression of transportersポスター発表
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議Biodiesel production using whole-cell biocatalyst of Aspergillus oryzae coexpression lipasesポスター発表
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議Antibody-immobilized TiO2 nanoparticles for cancer therapyポスター発表
- APBioChEC'09, 2009年11月, 英語, APBioChEC'09 Committee, 神戸市, 国際会議Affibody displaying bionanocapsules for HER2 specific drug deliveryポスター発表
- バイテクノロジー講演会, 2009年10月, 日本語, バイオテクノロジー研究会, 福井市, 国内会議セルロース資源からのバイオエタノール生産技術口頭発表（招待・特別）
- 化学工学会第41回秋季大会, 2009年09月, 日本語, 化学工学会, 広島大学, 国内会議糖代謝ネットワークを強化した酵母による混合糖からのバイオエタノール生産口頭発表（一般）
- 第61回日本生物工学会大会, 2009年09月, 日本語, (社)日本生物工学会, 名古屋市, 国内会議生デンプンから高効率にエタノールを生産する新規酵母の創製口頭発表（一般）
- 第41回 化学工学会秋季大会, 2009年09月, 日本語, (社)化学工学会, 東広島市, 国内会議菌体触媒を用いたバイオディーゼル生産におけるイオン液体の利用口頭発表（一般）
- 第61回日本生物工学会大会, 2009年09月, 日本語, (社)日本生物工学会, 名古屋市, 国内会議癌細胞標的化を目指したAffibody 提示ナノカプセルの開発口頭発表（一般）
- 第61回日本生物工学会大会, 2009年09月, 日本語, (社)日本生物工学会, 名古屋市, 国内会議遺伝子組換え麹菌を用いたタンパク質大量分泌生産技術の開発口頭発表（一般）
- 化学工学会第41回秋季大会, 2009年09月, 日本語, 化学工学会, 東広島市, 国内会議ヘマトコッカス藻の高密度培養に対する二酸化チタン-超音波励起法の適用ポスター発表
- Progress on Post Genome Technologies, 2009年09月, 英語, Tsinghua, Southeast, Nanjing, Northwest University, Beijing, China, 国際会議Selection of DNA aptamers using atomic force microscopy口頭発表（一般）
- 第61回日本生物工学会大会, 2009年09月, 日本語, (社)日本生物工学会, 名古屋市, 国内会議Porin を用いたコリネ菌新規細胞表層提示技術の開発口頭発表（一般）
- SIM2009 Annual Meeting and Exhibition, 2009年07月, 英語, Society for Industrial Microbiology, Toronto, Canada, 国際会議Development of environmentally benign and consolidated process for efficient production of cellulosic ethanol[招待有り]口頭発表（招待・特別）
- (財)不二たん白質研究振興財団第12回研究報告会, 2009年06月, 日本語, (財)不二たん白質研究振興財団, 豊中市, 国内会議大豆イソフラボンからのイソフラボンアグリコンの効率的生産に適した新規βグルコシダーゼの開発口頭発表（一般）
- 化学工学会 第75年会, 2009年03月, 日本語, 化学工学会, 神奈川県横浜市, 国内会議放線菌遺伝子組み換え系のタンパク質分泌生産への適用口頭発表（一般）
- 化学工学会 第74年会, 2009年03月, 日本語, 化学工学会, 神奈川県横浜市, 国内会議非侵襲性がん治療のためのタンパク質修飾ナノ粒子の開発口頭発表（一般）
- 農芸化学会2009年度大会, 2009年03月, 日本語, 日本農芸化学会, 福岡県福岡市, 国内会議バイオエタノール生産を目指したスーパー酵母の創製口頭発表（一般）
- 日本草地学会, 2009年03月, 日本語, 日本草地学会, 神奈川県藤沢市, 国内会議ソフトセルロース資源からのエタノール発酵技術の開発口頭発表（招待・特別）
- 農芸化学会2009年度大会, 2009年03月, 日本語, 日本農芸化学会, 福岡県福岡市, 国内会議キシロース資化性遺伝子組換え酵母の代謝プロファイリング解析ポスター発表
- 農芸化学会2009年度大会, 2009年03月, 日本語, 日本農芸化学会, 福岡県福岡市, 国内会議キシロースからの高効率エタノール生産を目指した宿主酵母株の検討ポスター発表
- 農芸化学会2009年度大会, 2009年03月, 日本語, 日本農芸化学会, 福岡県福岡市, 国内会議アミラーゼ発現酵母による生デンプンからの効率的な繰り返し発酵プロセスの開発ポスター発表
- 化学工学会 第74年会, 2009年03月, 日本語, 化学工学会, 神奈川県横浜市, 国内会議TiO&2&ナノ粒子と超音波を併用した新規がん治療法構築のための基礎的検討口頭発表（一般）
- 第31回日本分子生物学会年会;第81回日本生化学会大会 合同大会, 2008年12月, 日本語, 日本分子生物学学会, 日本生化学会, 神戸市, 国内会議未来バイオ燃料生産用スーパー(CBP)微生物の開発口頭発表（一般）
- 第31回日本分子生物学会年会・第82回日本生化学会大会 合同大会, 2008年12月, 日本語, 日本分子生物学学会, 日本生化学会, 神戸市, 国内会議二酸化チタンナノ粒子による活性酸素非依存的細胞死誘導口頭発表（一般）
- 若手フロンティア研究会2008, 2008年12月, 日本語, 神戸大学研究基盤センター, 神戸市, 国内会議原子間力顕微鏡を用いたアプタマーの選抜ポスター発表
- 若手フロンティア研究会2008, 2008年12月, 日本語, 神戸大学研究基盤センター, 神戸市, 国内会議温度応答性磁性ナノ粒子を用いた迅速かつ高効率なアフィニティー分子の選択法の確立ポスター発表
- 4th International Symposium on Biocatalysis and Biotechnology, 2008年11月, 英語, International Society of Biocatalysis and Biotechnology, Taipei, Taiwan, 国際会議Production of bio-fuels and chemicals from biomass by cell surface engineered yeast strains[招待有り]口頭発表（招待・特別）
- 4th Korea-Japan Workshop on Combinatorial Bioengineering, 2008年11月, 英語, Association of Combinatorial Bioengineering, Kookmin University, Seoul, 国際会議Methylester Synthesis By Fungi Immobilized BSPs in Ionic Liquidポスター発表
- 4th Korea-Japan Workshop on Combinatorial Bioengineering, 2008年11月, 英語, Association of Combinatorial Bioengineering, Seoul, Korea, 国際会議Efficient and direct ethanol production from raw starch by d-integrant polyploid fusants of Saccharomyces cerevisiaeポスター発表
- 4th Korea-Japan Workshop on Combinatorial Bioengineering, 2008年11月, 英語, Association of Combinatorial Bioengineering, Seoul, Korea, 国際会議Biorefinery strategy by cell surface engineered yeast strainsポスター発表
- 第17回日本バイオイメージング学会, 2008年10月, 日本語, 日本バイオイメージング学会, 千葉市, 国内会議特異性改変型バイオナノカプセルを用いたバイオイメージング及びDDSへの応用口頭発表（一般）
- International Workshop on Process Intensification 2008, 2008年10月, 英語, 化学工学会粒子流体プロセス部会, 化学工学会システム・情報・シミュレーション部会, 化学工学会反応工学部会, 化学工学会マイクロ化学研究会, 東京工業大学, 国際会議Improvement of Yeast Xylose Uptake Ability for Efficient Bioethanol Productionポスター発表
- 化学工学会 第40回 秋季大会, 2008年09月, 日本語, 化学工学会, 宮城県仙台市, 国内会議セルラーゼ発現酵母によるセルロースからのバイオエタノール生産ポスター発表
- 第60回 日本生物工学会2008年度大会, 2008年08月, 日本語, 日本生物工学会, 宮城県仙台市, 国内会議無蒸煮デンプンから効率的にエタノールを生産する高機能酵母の創製口頭発表（一般）
- 第60回 日本生物工学会2010年度大会, 2008年08月, 日本語, 日本生物工学会, 宮城県仙台市, 国内会議遺伝子組み換え放線菌を用いたセルラーゼ発現システムの構築口頭発表（一般）
- 第60回 日本生物工学会2009年度大会, 2008年08月, 日本語, 日本生物工学会, 宮城県仙台市, 国内会議リン脂質代謝酵素のイオン液体中での反応解析口頭発表（一般）
- 第60回 日本生物工学会2008年度大会, 2008年08月, 日本語, 日本生物工学会, 宮城県仙台市, 国内会議キチン結合ドメインを用いた麹菌細胞表層提示技術の開発口頭発表（一般）
- 第60回 日本生物工学会2008年度大会, 2008年08月, 日本語, 日本生物工学会, 宮城県仙台市, 国内会議Streptomyces griseocarneus NBRC13471由来スフィンゴミエリナーゼC の遺伝子クローニングと発現口頭発表（一般）
- American Society for Microbiology 108th General Meeting, 2008年06月, 英語, American Society for Microbiology, Boston, USA, 国際会議Oxidative-stress-induced metabolite production with sonocatalytic formation of reactive oxygen speciesポスター発表
- 化学工学会第73年会, 2008年03月, 日本語, 化学工学会, 浜松市, 国内会議フローサイトメーター(FCM)を利用した酵母GPCRシグナリング解析技術口頭発表（一般）
- 第6回最先端バイオテクノロジー若手発表会, 2008年02月, 日本語, 化学工学会関西支部化学工学会バイオ部会, 西宮市, 国内会議酵母GPCRシグナリングにおけるフローサイトメトリー解析技術ポスター発表
- 第6回 最先端バイオテクノロジー公開セミナー, 2008年02月, 日本語, 化学工学会関西支部、化学工学会バイオ部会, 西宮, 国内会議ストレス応答を利用したHaematococcus pluvialisによるアスタキサンチン生産の高効率化口頭発表（一般）
- ISSS2007, 2007年12月, 英語, ソノケミストリー学会, 京都, 国際会議Ultrasonic facilitation of OH radical generation in the presence of TiO2口頭発表（一般）
- ISSS2007, 2007年12月, 英語, ソノケミストリー学会, 京都, 国際会議The cancer cell injury by combination of protein displayed titanium dioxide nanoparticle and ultrasound irradiation口頭発表（一般）
- ISSS2007, 2007年12月, 英語, ソノケミストリー学会, 京都, 国際会議Construction of biomolecule-immobilized TiO2 nanoparticle for applying to new cell injuring method with ultrasound irradiation口頭発表（一般）
- ISSS2007, 2007年12月, 英語, ソノケミストリー学会, 京都, 国際会議Analysis of Sonodynamic Sterilization of Escherichia coli at the Surface of TiO2口頭発表（一般）
- ISSS2007, 2007年12月, 英語, ソノケミストリー学会, 京都, 国際会議Analysis of H2O2 formation induced by ultrasonic irradiation with TiO2. K口頭発表（一般）
- YABEC2007, 2007年10月, 英語, Young Asian Biochemical Engineers Community, Seoul, Korea, 国際会議Oxidative treatment of H. pluvialis with the sonocatalytic reactionポスター発表
- YABEC2007, 2007年10月, 英語, YABEC, Souel 韓国, 国際会議Application of biomolecule-immobilized TiO2 nanoparticle for chemical compound degradation and mammalian cell killing口頭発表（一般）
- 日本生物工学会第59回大会, 2007年09月, 日本語, 日本生物工学会, 東広島, 国内会議放線菌を宿主とするリン脂質代謝酵素の変異体解析口頭発表（一般）
- 日本生物工学会第59回大会, 2007年09月, 日本語, 日本生物工学会, 東広島, 国内会議放線菌Streptomyces lividans における分泌シグナルのタンパク生産に及ぼす影響解析口頭発表（一般）
- 第59回生物工学会大会, 2007年09月, 日本語, 日本生物工学会, 広島, 国内会議二酸化チタン-超音波励起法を利用したヘマトコッカス藻によるアスタキサンチン生産の誘導口頭発表（一般）
- ECB13, 2007年09月, 英語, ECB, バルセロナ スペイン, 国際会議Over-expression system of secretory phospholipase D by Streptomyces lividans口頭発表（一般）
- The 2nd International Workshop on Approaches to Single-Cell Analysis, 2007年09月, 英語, 東京, 国際会議New SELEX strategy for screening of DNA aptamer by AFM.口頭発表（一般）
- ECB13, 2007年09月, 英語, ECB, バルセロナ スペイン, 国際会議Induction of astaxantin biosynthesis in H. pluvialis using TiO2 and ultrasounds口頭発表（一般）
- 13th European Congress on Biotechnology, 2007年09月, 英語, European Federation of Biotechnology, Barcelona, Spain, 国際会議Induction of astaxanthin biosynthesis in H. pluvialis using TiO2 and ultrasoundsポスター発表
- 2nd International Congress on Ionic Liquids (COIL-2), 2007年08月, 英語, 横浜, 国際会議Phospholipid conversion in ionic liquid by phospholipase D口頭発表（一般）
- 化学工学会第72年会, 2007年03月, 日本語, 化学工学会, 京都, 国内会議放線菌の新規タンパク質発現系構築に関する研究口頭発表（一般）
- 化学工学会第72年会, 2007年03月, 日本語, 化学工学会, 京都, 国内会議組織ターゲティング機能を有する二酸化チタンナノ粒子によるがん細胞障害口頭発表（一般）
- 日本化学会第87春季年会, 2007年03月, 日本語, 日本化学会, 吹田, 国内会議リン脂質代謝酵素の酵素活性に及ぼすイオン液体の効果口頭発表（一般）
- 日本化学会第87春季年会, 2007年03月, 日本語, 日本化学会, 吹田, 国内会議8OH-dGを認識するDNAアプタマーの機能評価口頭発表（一般）
- 9th Japan-China-Korea Joint symposium on Enzyme Engineering, 2006年11月, 英語, 酵素工学研究会, 大津, 国際会議Structural analysis of 8OH-dG DNA aptamer口頭発表（一般）
- YABEC2006, 2006年11月, 英語, YABEC, 高雄, 国際会議Over-expression system for secretory enzyme by Streptomyces lividans口頭発表（一般）
- 9th Japan-China-Korea Joint symposium on Enzyme Engineering, 2006年11月, 英語, 酵素工学研究会, 大津, 国際会議Effect of solvent and ionic liquid on phospholipid conversion in bi-phase system by phospholipase D口頭発表（一般）
- 9th Japan-China-Korea Joint symposium on Enzyme Engineering, 2006年11月, 英語, 酵素工学研究会, 大津, 国際会議Construction of secretional expression system by Streptomyces lividans口頭発表（一般）
- 第15回ソノケミストリー討論会, 2006年10月, 日本語, ソノケミストリー学会, 金沢, 国内会議二酸化チタン超音波触媒法を応用した有害微生物殺菌システム口頭発表（一般）
- 第15回ソノケミストリー討論会, 2006年10月, 日本語, ソノケミストリー学会, 金沢, 国内会議二酸化チタン超音波触媒法による細胞死誘導口頭発表（一般）
- 第15回ソノケミストリー討論会, 2006年10月, 日本語, ソノケミストリー学会, 金沢, 国内会議二酸化チタン・超音波法によるOHラジカルの生成と溶存アルゴンの促進効果口頭発表（一般）
- 第15回ソノケミストリー討論会, 2006年10月, 日本語, ソノケミストリー学会, 金沢, 国内会議二酸化チタンへの超音波照射による過酸化水素生成機構の解析口頭発表（一般）
- 日本生物工学会第58回大会, 2006年09月, 日本語, 日本生物工学会, 豊中, 国内会議放線菌を用いたホスホリパーゼD酵素の遺伝子組み換え生産の培養特性解析に関する研究口頭発表（一般）
- 化学工学会第38回秋季大会, 2006年09月, 日本語, 化学工学会, 福岡, 国内会議二酸化チタン・超音波法によるOHラジカルの生成と溶存アルゴンの促進効果口頭発表（一般）
- 日本生物工学会第58回大会, 2006年09月, 日本語, 日本生物工学会, 豊中, 国内会議二酸化チタン・超音波触媒法によるがん細胞障害と増殖抑制口頭発表（一般）
- 日本生物工学会第58回大会, 2006年09月, 日本語, 日本生物工学会, 豊中, 国内会議組織認識能を持つ二酸化チタン内包リポソームの創製口頭発表（一般）
- 日本生物工学会第58回大会, 2006年09月, 日本語, 日本生物工学会, 豊中, 国内会議生体ストレスマーカー分子8OH-dGを認識するDNAアプタマーに関する研究口頭発表（一般）
- 化学工学会第38回秋季大会, 2006年09月, 日本語, 化学工学会, 福岡, 国内会議8OH-dGを認識するDNAアプタマーに関する基礎研究口頭発表（一般）
- 化学工学会第71年会, 2006年03月, 日本語, 化学工学会, 東京, 国内会議二酸化チタン・超音波触媒法によるがん細胞傷害と増殖抑制口頭発表（一般）
- 化学工学会第71年会, 2006年03月, 日本語, 化学工学会, 東京, 国内会議組織認識能をもつ二酸化チタン内包リポソームの創製口頭発表（一般）
- 化学工学会第71年会, 2006年03月, 日本語, 化学工学会, 東京, 国内会議リン脂質代謝酵素の蓋構造変化における活性相関に関する研究(II)口頭発表（一般）
- 日本生物工学会第57回大会, 2005年11月, 日本語, 日本生物工学会, 筑波, 国内会議放線菌Streptomyces cinnamoneum由来発現プロモーターを用いたタンパク質発現系構築に関する研究口頭発表（一般）
- 日本生物工学会第57回大会, 2005年11月, 日本語, 日本生物工学会, 筑波, 国内会議リン脂質代謝酵素ホスホリパーゼDの蓋構造の活性相関に関する研究口頭発表（一般）
- 第14回ソノケミストリー討論会, 2005年10月, 日本語, ソノケミストリー学会, 豊橋, 国内会議有害微生物の超音波殺菌に対する二酸化チタンの増強効果口頭発表（一般）
- 化学工学会第70年会, 2005年03月, 日本語, 化学工学会, 名古屋, 国内会議放線菌を宿主とするタンパク質発現系の構築口頭発表（一般）
- 化学工学会第70年会, 2005年03月, 日本語, 化学工学会, 名古屋, 国内会議リン脂質代謝酵素の蓋構造変化における活性相関に関する研究口頭発表（一般）
- Bioseparation2004, 2004年10月, 英語, 化学工学会, 福岡, 国際会議Screening of DNA aptamer that recognizes nucleotide derivative口頭発表（一般）
- 10thAPCChE, 2004年10月, 英語, 化学工学会, 北九州, 国際会議Investigation of the Catalytic Function of Phospholipase D by Site Directed Mutagenesis口頭発表（一般）
- 10thAPCChE, 2004年10月, 英語, 化学工学会, 北九州, 国際会議Cell Disinfection by Ultrasonic Irradiation with TiO2 Photocatalyst口頭発表（一般）
- 化学工学会山口下関大会, 2004年09月, 日本語, 化学工学会, 下関, 国内会議低分子化合物を認識するDNAアプタマーの取得とそのバイオセンサーへの応用口頭発表（一般）
- 日本生物工学会第56回大会, 2004年09月, 日本語, 日本生物工学会, 名古屋, 国内会議生理活性物質を認識するDNAアプタマーの探索とその機能評価口頭発表（一般）
- YABEC2004, 2004年09月, 英語, YABEC, 大阪, 国際会議Screening of DNA aptamer that recognizes nucleotide derivative口頭発表（一般）
- 化学工学会第69年会, 2004年04月, 日本語, 化学工学会, 堺, 国内会議有機溶媒感受性プロモーターを用いた水質評価法の構築口頭発表（一般）
- 1st PRICPS, 2004年04月, 英語, 日本蛋白質学会, 横浜, 国際会議Investigation of catalytic function of phospholipase D enzyme by site directed mutagenesis口頭発表（一般）
- 化学工学会第69年会, 2004年04月, 英語, 化学工学会, 堺, 国内会議Disinfection of Legionella by sonocatalytic irradiation with TiO2 photocatalyst口頭発表（一般）
- 化学工学会京都大会, 2003年12月, 日本語, 化学工学会, 京都, 国内会議新規ラジカル生成法の環境浄化と医療への応用口頭発表（一般）
- YABEC2003, 2003年11月, 英語, YABEC, 済洲島, 国際会議Screening of DNA aptamer that recognizes phenol molecule.口頭発表（一般）
- 第12回ソノケミストリー討論会, 2003年10月, 日本語, ソノケミストリー学会, 福岡, 国内会議二酸化チタン・超音波照射法による大腸菌殺菌メカニズムの解析口頭発表（一般）
- 日本生物工学会第55回大会, 2003年09月, 日本語, 日本生物工学会, 熊本, 国内会議放線菌由来リン脂質代謝酵素の変異体の機能解析口頭発表（一般）
- European Conference of Biotechnology (ECB) 11th, 2003年08月, 英語, ECB, バーゼル・スイス, 国際会議Investigation of catalytic function of phospholipase D enzyme by site directed mutagenesis口頭発表（一般）
- 化学工学会第68年会, 2003年03月, 日本語, 化学工学会, 東京, 国内会議分子進化法を用いたPLDの触媒機能変換口頭発表（一般）
- 化学工学会第68年会, 2003年03月, 日本語, 化学工学会, 東京, 国内会議機能性DNA分子の探索と水質評価への応用口頭発表（一般）
- YABEC2002, 2002年11月, 英語, YABEC, 台北, 国際会議Bio-sensing of benzene derivatives using Ps promoter gene and GFP as a reporter protein (II)口頭発表（一般）
- 日本生物工学会第54回大会, 2002年10月, 日本語, 日本生物工学会, 大阪, 国内会議有機溶媒感受性プロモーターを用いた水質評価法の構築口頭発表（一般）
- 日本生物工学会第54回大会, 2002年10月, 日本語, 日本生物工学会, 大阪, 国内会議リン脂質代謝酵素ホスホリパーゼDの遺伝子工学的手法による触媒機能解析口頭発表（一般）
- 化学工学会第35回秋季大会, 2002年09月, 日本語, 化学工学会, 神戸, 国内会議有機化合物認識遺伝子群を用いた水質評価法の構築口頭発表（一般）
- 化学工学会第35回秋季大会, 2002年09月, 日本語, 化学工学会, 神戸, 国内会議変異型ホスホリパーゼDを用いた新規リン脂質合成への検討口頭発表（一般）
- 化学工学会第35回秋季大会, 2002年09月, 日本語, 化学工学会, 神戸, 国内会議二酸化チタン界面での超音波力学的殺菌メカニズムの解析口頭発表（一般）
- 化学工学会第35回秋季大会, 2002年09月, 日本語, 化学工学会, 神戸, 国内会議二酸化チタン・超音波法による内分泌撹乱物質の分解処理に関する研究口頭発表（一般）
- 化学工学会第67年会, 2002年03月, 日本語, 化学工学会, 福岡, 国内会議有機溶媒感受性プロモーターを用いた水質評価法の構築口頭発表（一般）
- 化学工学会第67年会, 2002年03月, 日本語, 化学工学会, 福岡, 国内会議二酸化チタン超音波照射法によるヒドロキシラジカルの生成と内分泌撹乱物質の分解処理口頭発表（一般）
- 化学工学会第67年会, 2002年03月, 日本語, 化学工学会, 福岡, 国内会議ホスホリパーゼDにおける構造変化とリン脂質合成相関に関する解析口頭発表（一般）
- YABEC2001, 2001年10月, 英語, YABEC, 上海, 国際会議Construction of functional phospholipid synthesis system by using mutant phospholipase D enzyme口頭発表（一般）
- YABEC2001, 2001年10月, 英語, YABEC, 上海, 国際会議Construction of benzene derivatives biosensor using Ps promoter gene and GFP as a reporter gene口頭発表（一般）
- 化学工学会福井大会, 2001年07月, 日本語, 化学工学会, 福井, 国内会議ホスホリパーゼDの触媒機構解析と新規リン脂質合成への応用口頭発表（一般）
- 化学工学会第66年会, 2001年04月, 日本語, 化学工学会, 東広島, 国内会議レポーター遺伝子組換え微生物を用いる水質評価法の構築口頭発表（一般）
- 化学工学会第66年会, 2001年04月, 日本語, 化学工学会, 東広島, 国内会議リン脂質代謝酵素ホスホリパーゼD の触媒機構及び活性化機構の解析口頭発表（一般）
- YABEC2000, 2000年11月, 英語, YABEC, 福岡, 国際会議Improved catalytic properties of phospholipase D by site directed mutagenesis口頭発表（一般）
- 化学工学会秋季大会, 2000年09月, 英語, 化学工学会, 浜松, 国内会議Secretory Overexpression of Phospholipase D By Recombinant Streptomyces lividans口頭発表（一般）
- 日本生物工学会第52回大会, 2000年08月, 日本語, 日本生物工学会, 札幌, 国内会議遺伝子工学的手法による活性変異型ホスホリパーゼDの構築と解析口頭発表（一般）
- 日本生物工学会第51回大会, 1999年09月, 日本語, 日本生物工学会, 吹田市, 国内会議放線菌Stv. cinnamoneum由来PLDの発現系構築と触媒機構解析口頭発表（一般）
- 学術研究助成基金助成金／基盤研究(C), 2019年04月 - 2022年03月過酸化チタンナノ粒子を用いたTheranostic粒子線治療の開発競争的資金
- 学術研究助成基金助成金／基盤研究(C), 2017年04月 - 2020年03月競争的資金
- 科学研究費補助金／基盤研究(B), 2016年04月 - 2019年03月, 研究代表者競争的資金
- 学術研究助成基金助成金／挑戦的萌芽研究, 2016年04月 - 2019年03月競争的資金
- 国立研究開発法人科学技術振興機構, 国際科学技術共同研究推進事業・地球規模課題対応国際科学技術協力プログラム（SATREPSインドネシア）, 2017年11月 - 2018年03月, 研究代表者【JST-SATREPS】インドネシアにおける統合バイオリファイナリーシステムの開発競争的資金
- 国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業（CREST）, 2013年 - 2018年, 研究代表者【CREST】植物バイオマスを原料とした芳香族ポリマー原料の発酵生産競争的資金
- 国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業（先端的低炭素化技術開発ALCA）, 2017年, 研究代表者【ALCA】イオン液体前処理バイオマスの酵素糖化、および得られる糖液の特性評価競争的資金
- 学術研究助成基金助成金／基盤研究(C), 2013年04月 - 2016年03月, 研究代表者競争的資金
- 学術研究助成基金助成金／挑戦的萌芽研究, 2013年04月 - 2016年03月競争的資金
- 戦略的イノベーション創造プログラム（SIP）, 2016年, 研究代表者酵素法による植物油からのBDFと副産物バイオマスへの変換（基礎条件検討）競争的資金
- 国際科学技術共同研究推進事業・地球規模課題対応国際科学技術協力プログラム（SATREPSインドネシア）, 2016年, 研究代表者【JST-SATREPS】インドネシアにおける統合バイオリファイナリーシステムの開発競争的資金
- 国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業（CREST）, 2016年, 研究代表者【CREST】植物バイオマスを原料とした芳香族ポリマー原料の発酵生産競争的資金
- 国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業（ALCA）, 2016年, 研究代表者【ALCA】イオン液体前処理バイオマスの酵素糖化、および得られる糖液の特性評価競争的資金
- 科学研究費補助金／基盤研究(B), 2012年04月 - 2015年03月競争的資金
- 科学研究費補助金／基盤研究(B), 2012年04月 - 2015年03月競争的資金
- 地球規模課題対応国際科学技術協力プログラム, 2015年, 研究代表者SATREPS「インドネシアにおける統合バイオリファイナリーシステムの開発」競争的資金
- 地球規模課題対応国際科学技術協力プログラム, 2014年, 研究代表者SATREPS「インドネシアにおける統合バイオリファイナリーシステムの開発」競争的資金
- 二国間交流事業（インドネシアとの共同研究）, 2013年, 研究代表者二国間交流「酵母細胞表層工学によるエタノール・マンノオリゴ糖生産の統合バイオプロセス開発」競争的資金
- 地球規模課題対応国際科学技術協力プログラム, 2013年, 研究代表者SATREPS「統合バイオリファイナリー研究拠点構築プロジェクト（インドネシア）」競争的資金
- 二国間交流事業（インドネシアとの共同研究）, 2012年, 研究代表者二国間交流「酵母細胞表層工学によるエタノール・マンノオリゴ糖生産の統合バイオプロセス開発」競争的資金
- 独立行政法人新エネルギー・産業技術総合開発機構, バイオマスエネルギー等高効率転換技術開発, 2012年, 研究代表者イオン液体を利用したバイオマスからのバイオ燃料生産技術の開発競争的資金
- 地球規模課題対応国際科学技術協力プログラム, 2012年, 研究代表者SATREPS「インドネシアにおける統合バイオリファイナリーシステムの開発」競争的資金
- 二国間交流事業（インドネシアとの共同研究）, 2011年, 研究代表者二国間交流「酵母細胞表層工学によるエタノール・マンノオリゴ糖生産の統合バイオプロセス開発」競争的資金
- 学術研究助成基金助成金／挑戦的萌芽研究, 2011年競争的資金
- 独立行政法人新エネルギー・産業技術総合開発機構, バイオマスエネルギー等高効率転換技術開発, 2011年, 研究代表者イオン液体を利用したバイオマスからのバイオ燃料生産技術の開発競争的資金
- 2010年, 研究代表者産業技術研究「高分解能生体分子プローブカンチレバーの創製による生体認識イメージング技術の開発」競争的資金
- 科学研究費補助金／若手研究(B), 2009年, 研究代表者競争的資金
- 2009年, 研究代表者産業技術研究「高分解能生体分子プローブカンチレバーの創製による生体認識イメージング技術の開発」競争的資金
- 2008年, 研究代表者産業技術研究「高分解能生体分子プローブカンチレバーの創製による生体認識イメージング技術の開発」（２）競争的資金
- 2008年, 研究代表者産業技術研究「高分解能生体分子プローブカンチレバーの創製による生体認識イメージング技術の開発」（１）競争的資金
- 科学研究費補助金／若手研究(B), 2007年, 研究代表者競争的資金
- 2007年, 研究代表者放射菌によるP450系酵素群の大量発現系の開発競争的資金
- 科学研究費補助金／特定領域研究, 2007年, 研究代表者競争的資金
- 科学研究費補助金／特定領域研究, 2007年, 研究代表者競争的資金
- 糖液の製造方法及び多糖類系バイオマス由来化合物の製造方法特願2014-129051, 2014年06月24日, 大学長, 特許6403188, 2018年09月21日特許権
- βグルコシダーゼを発現する大腸菌特願2010-258361, 2010年11月18日, 大学長, 特許5846523, 2015年12月04日特許権
- 放射線治療剤特願2012-500638, 2011年02月17日, 大学長, 特許5649137, 2014年11月21日特許権
- 放射線治療剤(中)201180008541.3, 2011年02月17日, 大学長, ZL201180008541.3, 2014年07月02日特許権
- 放射線治療剤13/578836, 2011年02月17日, 大学長, 8580312, 2013年11月12日特許権
- セルロース含有材料からの有用物質の生産方法特願2009-183880, 2009年08月06日, 大学長, 特許5120965, 2012年11月02日特許権
研究シーズ
■ 研究シーズ- 微生物の力によるバイオマスからのバイオ燃料や化学品生産シーズカテゴリ：ライフサイエンス, 環境・農学, ナノテク・材料研究キーワード：バイオマス, 微生物変換, バイオ燃料, バイオリファイナリー, バイオナノテクノロジー研究内容：地球温暖化の防止に向けて、カーボンリサイクル技術の開発が求められています。私の研究では、空気中の二酸化炭素を固定化した様々なバイオマス(植物)を原料として、微生物の力を用いて、バイオ燃料や化学品へと変換する技術の開発を進めております。バイオマスの豊富な東南アジアの国々の研究者らと連携を行い、実バイオマスを用いた物質生産への検討を進めております。全く異なりますが、ナノ粒子を用いたがん治療の研究も進めております。