Research activity information

• Oct. 2021 神戸大学, 第13回学長表彰（財務貢献者）

  荻野 千秋


• Oct. 2019 神戸大学, 第11回学長表彰（財務貢献者）

  荻野 千秋


• Oct. 2018 神戸大学, 第10回学長表彰（財務貢献者）

  荻野 千秋


• Oct. 2017 神戸大学, 第9回学長表彰（財務貢献者）

  荻野 千秋


• Sep. 2013 日本生物工学会, 生物工学論文賞, Direct isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display system

  SOMA YUKI, INOKUMA KENTARO, TANAKA TSUTOMU, OGINO CHIAKI, KONDO AKIHIKO, OKAMOTO MASAHIRO, HANAI TAIZO

  Japan society


• Mar. 2007 化学工学会, 平成18年度化学工学会 奨励賞(實吉雅郎記念賞), リン脂質修飾酵素ホスホリパーゼＤに関する基礎解析および生産技術の開発

  OGINO Chiaki

• Design of a thermostable bilirubin oxidase from Myrothecium verrucaria

  Horiguchi, H.K., Yamada, S., Semba, H., Tsuboi, H., Bogaki, T., Koda, A., Ishikawa, K., Mori, Y., Ogino, C., Takagi, M., Tsujino, Y.

  2025, Journal of Bioscience and Bioengineering

  Scientific journal


• Lipid Production in Streptomyces jeddahensis Is Enhanced by Glucose and Fatty Acid Derivatives, with Temperature Variations Influencing Gene Expression and Biosynthesis

  Apriliana, P., Kahar, P., Rachmadona, N., Restu, W.K., Kondo, A., Ogino, C.

  2025, Fermentation, 11(2) (2)

  Scientific journal


• Efficient isoprimeverose production using an enzyme cocktail from engineered Aspergillus oryzae and yeast-assisted purification

  Wakai, S., Nakashima, N., Tsutsumi, H., Hata, Y., Baihaqqi, F., Kondo, A., Ogino, C.

  2025, Enzyme and Microbial Technology, 190

  Scientific journal


• Lipid Production from Palm Acid Oil (PAO) as a Sole Carbon Source by Meyerozyma guilliermondii

  Zain, N.-A.A., Tan, K.L., Kahar, P., Ogino, C.

  2025, Processes, 13(2) (2)

  Scientific journal


• Microbial Electrosynthesis for Sustainable Polyhydroxybutyrate Production from CO2 Using Bismuth Nanoparticles

  Aliyah, A., Putra, F.J.N., Minamimoto, H., Mori, Y., Kahar, P., Syauqi, M.I., Ogino, C.

  2025, Chemelectrochem

  Scientific journal


• Maleic Acid-Butanol Pretreatment to Enhance Cellulose Accessibility for Enzymatic Hydrolysis and Ethanol Production from Oil Palm Empty Fruit Bunch

  Postiaux, A., Putra, F.J.N., Kahar, P., Richel, A., Ogino, C.

  2025, ACS Environmental Au, 5(1) (1)

  Scientific journal


• Factors Affecting D-Lactic Acid Production by Flocculant Saccharomyces cerevisiae Under Non-Neutralizing Conditions

  Rahmasari, D., Kahar, P., de Oliveira, A.V., Putra, F.J.N., Kondo, A., Ogino, C.

  2025, Microorganisms, 13(3) (3)

  Scientific journal


• Catalase-Knockout Complements the Radio-Sensitization Effect of Titanium Peroxide Nanoparticles on Pancreatic Cancer Cells

  Tasia, W., Washio, A., Yamate, K., Morita, K., Mori, Y., Kahar, P., Sasaki, R., Ogino, C.

  2025, Molecules, 30(3) (3)

  Scientific journal


• Important Key Points Regarding the Circular Bioeconomy in the Palm Oil Industry: Current Practices and Future Perspectives

  Kahar, P., Zain, N.-A.A., Pangestu, R., Ogino, C.

  2025, Palm Oil Export Market Trends Challenges and Future Strategies for Sustainability

  Scientific journal


• Production of lignin monomeric alcohols and lipids from oil palm empty fruit bunch by a combination of alkaline nitrobenzene depolymerization and Lipomyces starkeyi bioconversion

  Putra, F.J.N., Kahar, P., Kondo, A., Ogino, C.

  Aug. 2024, Biochemical Engineering Journal, 208

  [Refereed]

  Scientific journal


• Editing Streptomyces genome using target AID system fused with UGI-degradation tag

  Apriliana, P., Kahar, P., Kashiwagi, N., Kondo, A., Ogino, C.

  Jun. 2024, Engineering in Life Sciences, 24(8) (8)

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Complete sequence randomness of lactate-based copolymers, LAHBs with varied lactate monomer fractions employing a series of propionyl-CoA transferases.

  Sangho Koh, Ryota Endo, Prihardi Kahar, Yutaro Mori, Chiaki Ogino, Shinji Tanaka, Shinji Tanaka, Yusuke Imai, Seiichi Taguchi

  Previously, we synthesized an evolved version of a bio-based polylactide (PLA) on microbial platforms using our engineered lactate-polymerizing enzyme (LPE). This lactate (LA)-based copolyester, LAHB, has advantages over PLA, including improved flexibility and biodegradability, and its properties can be regulated through the LA fraction. To expand the LA-incorporation capacity and improve polymer properties, in the state of in vivo LAHB production, propionyl-CoA transferases (PCTs) that exhibited enhanced production of LA-CoA than the conventional PCTs were selected. Here, the present study has demonstrated that the LA fraction of LAHB could be altered using various PCTs. Enhanced PCT performance was achieved by balancing polymer production and cell growth. Both events are governed by the use of acetyl-CoA, a commonly shared key metabolite. This could be attributed to the different reactivities of individual PCTs towards acetyl-CoA, which serves both as a CoA donor and a leading compound in the TCA cycle. Interestingly, we found complete sequence randomness in the LAHB copolymers, independent of the LA fraction. The mechanism of LA fraction-independent sequence randomness is discussed. This new PCT-based strategy synergistically combines with the evolution of LPE to advance the LAHB project, and enables us to perform advanced applications other than LAHB production utilizing CoA-linked substrates.

  Jun. 2024, International journal of biological macromolecules, 133055 - 133055, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Production of single cell oil by Lipomyces starkeyi from waste plant oil generated by the palm oil mill industry.

  Noor-Afiqah Ahmad Zain, Prihardi Kahar, Kumar Sudesh, Chiaki Ogino, Akihiko Kondo

  Only a few reports available about the assimilation of hydrophobic or oil-based feedstock as carbon sources by Lipomyces starkeyi. In this study, the ability of L. starkeyi to efficiently utilize free fatty acids (FFAs) and real biomass like palm acid oil (PAO) as well as crude palm kernel oil (CPKO) for growth and lipid production was investigated. PAO, CPKO, and FFAs were evaluated as sole carbon sources or in the mixed medium containing glucose. L. starkeyi was able to grow on the medium supplemented with PAO and FFAs, which contained long-chain length FAs and accumulated lipids up to 35% (w/w) of its dry cell weight. The highest lipid content and lipid concentration were achieved at 50% (w/w) and 10.1 g/L, respectively, when L. starkeyi was cultured in nitrogen-limited mineral medium (-NMM) supplemented with PAO emulsion. Hydrophobic substrate like PAO could be served as promising carbon source for L. starkeyi.

  May 2024, Journal of bioscience and bioengineering, English, Domestic magazine

  Scientific journal


• Comparative responses of flocculating and nonflocculating yeasts to cell density and chemical stress in lactic acid fermentation.

  Radityo Pangestu, Prihardi Kahar, Chiaki Ogino, Akihiko Kondo

  While flocculation has demonstrated its efficacy in enhancing yeast robustness and ethanol production, its potential application for lactic acid fermentation remains largely unexplored. Our study examined the differences between flocculating and nonflocculating Saccharomyces cerevisiae strains in terms of their metabolic dynamics when incorporating an exogenous lactic acid pathway, across varying cell densities and in the presence of lignocellulose-derived byproducts. Comparative gene expression profiles revealed that cultivating a nonflocculant strain at higher cell density yielded a substantial upregulation of genes associated with glycolysis, energy metabolism, and other key pathways, resulting in elevated levels of fermentation products. Meanwhile, the flocculating strain displayed an inherent ability to sustain high glycolytic activity regardless of the cell density. Moreover, our investigation revealed a significant reduction in glycolytic activity under chemical stress, potentially attributable to diminished ATP supply during the energy investment phase. Conversely, the formation of flocs in the flocculating strain conferred protection against toxic chemicals present in the medium, fostering more stable lactic acid production levels. Additionally, the distinct flocculation traits observed between the two examined strains may be attributed to variations in the nucleotide sequences of the flocculin genes and their regulators. This study uncovers the potential of flocculation for enhanced lactic acid production in yeast, offering insights into metabolic mechanisms and potential gene targets for strain improvement.

  Apr. 2024, Yeast (Chichester, England), 41(4) (4), 192 - 206, English, International magazine

  Scientific journal


• Engineering robust yeast for neutralizing-agent-free production of lactic acid from sugarcane bagasse hydrolysate

  Radityo Pangestu, Prihardi Kahar, Lutfi Kholida, Urip Perwitasari, Ahmad Thontowi, Fahrurrozi Fahrurrozi, Puspita Lisdiyanti, Yopi Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  Jan. 2024

  [Refereed]


• Evaluation of lipid production efficiency using palm oil mill effluent as a carbon source by Lipomyces starkeyi

  Rachmadona, N., Kahar, P., Juanssilfero, A.B., Nurrusyda, F.S., nd Dewi, D.A.S.L.A., Kurnia, I., Rahayu, I., Ogino, C.

  2024, Biomass Conversion and Biorefinery

  Scientific journal


• Development of a Lignin-Based Carrier - Citronella Oil Nanoemulsion and Its Utilization as an Herbicide against Specific Weed Species

  Restu, W.K., Aryana, N., Septiyanti, M., Triwulandari, E., Sampora, Y., Devy, Y.A., Muryanto, Ghozali, M., Meliana, Y., Sondari, D., Fatriasari, W., Nasir Mohamad, Ibrahim, M., Hazwan Hussin, M., Ogino, C.

  2024, Chemistry and Biodiversity, 21(9) (9)

  Scientific journal


• Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered Corynebacterium glutamicum.

  Taghreed Elkasaby, Dao Duy Hanh, Prihardi Kahar, Hideo Kawaguchi, Takashi Sazuka, Akihiko Kondo, Chiaki Ogino

  Itaconic acid is a promising biochemical building block that can be used in polymer synthesis. Itaconic acid is currently produced in industry by the natural producer fungus Aspergillus terreus using glucose as a main carbon source. Most research for itaconic acid production using lignocellulosic-based carbon sources was carried out by A. terreus. Engineered Corynebacterium glutamicum strain which can grow in presence of fermentation inhibitors without effect on growth, was used for production of itaconic acid using sweet sorghum juice and bagasse sugar lysate (BSL). BSL contains many inhibitors unlike sorghum juice. C. glutamicum could grow in the media containing both types of lignocellulose-based carbon sources without showing any growth inhibition, however, sorghum juice was better in itaconic acid production than BSL. Different constructed strains of C. glutamicum were used for itaconic acid production, however, C. glutamicum ATCC 13032 pCH-Tad1optAdi1opt strain expressing Adi1/Tad1 genes (trans-pathway) from Ustilago maydis proved to be better in itaconic acid production giving final titer of 8.4 and 4.02 g/L using sweet sorghum juice and BSL as the sole carbon sources by fed-batch fermentation. Our study is the first for production of itaconic acid using sweet sorghum juice and BSL. The present study also proved that C. glutamicum can be used for enhancing itaconic acid production using lignocellulosic-based carbon sources.

  Jan. 2024, Enzyme and microbial technology, 172, 110345 - 110345, English, International magazine

  Scientific journal


• Saccharification of Pretreated Empty Bunch by Concentrated Xylanase and Assisted by Cellulase

  Hans Wijaya, Prihardi Kahar, Kengo Sasaki, Nanik Rahmani, Euis Hermiati, Yopi, Chiaki Ogino, Bambang Prasetya, Rumella Simarmata, Margaretta Christita, Akihiko Kondo

  Dec. 2023, AIP Conference Proceedings, 2972(1) (1)

  International conference proceedings


• Nanoscopic lignin mapping on cellulose nanofibers via scanning transmission electron microscopy and atomic force microscopy

  Kenta Morita, Musashi Takenaka, Kohei Tomita, Jun Ishii, Hideo Kawaguchi, Daisuke Murakami, Hikaru Amo, Miku Fujii, Tatsuo Maruyama, Takuya Matsumoto, Takashi Nishino, Chiaki Ogino

  Abstract Cellulose has been developed as an alternative to petrochemical materials. By comparison with refined nanofibers (RCNFs), lignocellulose nanofibers (LCNFs) show particular promise because it is produced from biomass using only mild pretreatment. The mechanical properties of LCNFs depend on the contained lignin. However, the microscopic location of the lignin contained in LCNFs has not been determined. Thus, we developed two methods to detect and visualize lignin. One uses a scanning transmission electron microscope (STEM) equipped with an energy dispersive X-ray spectroscopy detector. The other method uses an atomic force microscope (AFM) equipped with a cantilever coated with an aromatic molecule. Both methods revealed that the lignin in LCNFs covers a thin cellulose fiber and is precipitated in a grained structure. In particular, the AFM system was able to determine the nanoscopic location of lignin-rich areas. The present study establishes a strong tool for analyzing the characteristics of lignin-containing materials. Graphical abstract

  Springer Science and Business Media LLC, Nov. 2023, Cellulose, 30(18) (18), 11357 - 11367

  Scientific journal


• The bioconversion of lignin derivative aldehydes into high-value aromatic alcohols and lipids via Lipomyces starkeyi

  Putra, F.J.N., Kahar, P., Kondo, A., Ogino, C.

  Nov. 2023, Biochemical Engineering Journal, 200

  [Refereed]

  Scientific journal


• Flocculation influences the response to increased cell density and chemical stresses during lactic acid fermentation in yeast

  Chiaki Ogino, Radityo Pangestu, Prihardi Kahar, Akihiko Kondo

  Jul. 2023


• Enhanced production of itaconic acid from enzymatic hydrolysate of lignocellulosic biomass by recombinant Corynebacteriumglutamicum.

  Dao Duy Hanh, Taghreed Elkasaby, Hideo Kawaguchi, Yota Tsuge, Chiaki Ogino, Akihiko Kondo

  Itaconic acid (IA) is a value-added chemical currently produced by Aspergillus terreus from edible glucose and starch but not from inedible lignocellulosic biomass owing to the high sensitivity to fermentation inhibitors present in the hydrolysate of lignocellulosic biomass. To produce IA from lignocellulosic biomass, a gram-positive bacterium, Corynebacterium glutamicum, with a high tolerance to fermentation inhibitors was metabolically engineered to express a fusion protein composed of cis-aconitate decarboxylase from A. terreus responsible for IA formation from cis-aconitate and a maltose-binding protein (malE) from Escherichia coli. The codon-optimized cadA_malE gene was expressed in C. glutamicum ATCC 13032, and the resulting recombinant strain produced IA from glucose. IA concentration increased 4.7-fold by the deletion of the ldh gene encoding lactate dehydrogenase. With the Δldh strain HKC2029, an 18-fold higher IA production was observed from enzymatic hydrolysate of kraft pulp as a model lignocellulosic biomass than from glucose (6.15 and 0.34 g/L, respectively). The enzymatic hydrolysate of kraft pulp contained various potential fermentation inhibitors involved in furan aldehydes, benzaldehydes, benzoic acids, cinnamic acid derivatives, and aliphatic acid. Whereas cinnamic acid derivatives severely inhibited IA production, furan aldehydes, benzoic acids, and aliphatic acid improved IA production at low concentrations. The present study suggests that lignocellulosic hydrolysate contains various potential fermentation inhibitors; however, some of them can serve as enhancers for microbial fermentation likely due to the changing of redox balance in the cell.

  Jul. 2023, Journal of bioscience and bioengineering, 136(1) (1), 7 - 12, English, Domestic magazine

  Scientific journal


• Effect of different metabolic pathways on itaconic acid production in engineered Corynebacterium glutamicum.

  Taghreed Elkasaby, Dao Duy Hanh, Hideo Kawaguchi, Akihiko Kondo, Chiaki Ogino

  Itaconic acid (IA), a C5-dicarboxylic acid, is a potential bio-based building block for the polymer industry. There are three pathways for IA production from natural IA producers; however, most of the engineered strains were used for IA production by heterologous expression of cis-aconitate decarboxylase gene (cadA) from Aspergillus terreus. In this study, IA was produced by an engineered Corynebacterium glutamicum ATCC 13032 expressing two different types of genes from two distinct pathways. The first involves the mammalian immunoresponsive gene1 (Irg1) derived from Mus musculus. The second (termed here the trans-pathway) involves two genes from the natural IA producer Ustilago maydis which are aconitate-delta-isomerase (Adi1) and trans-aconitate decarboxylase (Tad1) genes. The constructed strains developing the two distinct IA production pathways: C. glutamicum ATCC 13032 pCH-Irg1opt and C. glutamicum ATCC 13032 pCH-Tad1optadi1opt were used for production of IA from different carbon sources. The results reflect the possibility for IA production from C. glutamicum expressing the trans-pathway (Adi1/Tad1 genes) and cis-pathway (Irg1 gene) other than the well-known cis-pathway that depends mainly on cadA gene from A. terreus. The developed strain expressing trans-pathway from U. maydis; however, proved to be better at IA production with high titers of 12.25, 11.34, and 11.02 g/L, and a molar yield of 0.22, 0.42, and 0.43 mol/mol from glucose, maltose, and sucrose, respectively, via fed-batch fermentation. The present study suggests that trans-pathway is better than cis-pathway for IA production in engineered C. glutamicum.

  Jun. 2023, Journal of bioscience and bioengineering, 136(2) (2), 109 - 116, English, Domestic magazine

  Scientific journal


• Sucrose Signaling Contributes to the Maintenance of Vascular Cambium by Inhibiting Cell Differentiation.

  Aoi Narutaki, Prihardi Kahar, Shunji Shimadzu, Shota Maeda, Tomoyuki Furuya, Kimitsune Ishizaki, Hidehiro Fukaki, Chiaki Ogino, Yuki Kondo

  Plants produce sugars by photosynthesis and use them for growth and development. Sugars are transported from source-to-sink organs via the phloem in the vasculature. It is well known that vascular development is precisely controlled by plant hormones and peptide hormones. However, the role of sugars in the regulation of vascular development is poorly understood. In this study, we examined the effects of sugars on vascular cell differentiation using a vascular cell induction system named Vascular cell Induction culture System Using Arabidopsis Leaves (VISUAL). We found that sucrose has the strongest inhibitory effect on xylem differentiation among several types of sugars. Transcriptome analysis revealed that sucrose suppresses xylem and phloem differentiation from cambial cells. Physiological and genetic analysis suggested that sucrose might function through the BES1 transcription factor, which is the central regulator of vascular cell differentiation. Conditional overexpression of cytosolic invertase led to a decrease in the number of cambium layers due to an imbalance between cell division and differentiation. Taken together, our results suggest that sucrose potentially acts as a signal that integrates environmental conditions with the developmental program.

  May 2023, Plant & cell physiology, 64(12) (12), 1511 - 1522, English, Domestic magazine

  Scientific journal


• An analysis of sugary endosperm in sorghum: Characterization of mutant phenotypes depending on alleles of the corresponding starch debranching enzyme

  Shumpei Hashimoto, Satoshi Okada, Satoko Araki-Nakamura, Kozue Ohmae-Shinohara, Kotaro Miura, Hideo Kawaguchi, Chiaki Ogino, Shigemitsu Kasuga, Takashi Sazuka

  Sorghum is the fifth most important cereal crop. Here we performed molecular genetic analyses of the ‘SUGARY FETERITA’ (SUF) variety, which shows typical sugary endosperm traits (e.g., wrinkled seeds, accumulation of soluble sugars, and distorted starch). Positional mapping indicated that the corresponding gene was located on the long arm of chromosome 7. Within the candidate region of 3.4 Mb, a sorghum ortholog for maize Su1 (SbSu) encoding a starch debranching enzyme ISA1 was found. Sequencing analysis of SbSu in SUF uncovered nonsynonymous single nucleotide polymorphisms (SNPs) in the coding region, containing substitutions of highly conserved amino acids. Complementation of the rice sugary-1 (osisa1) mutant line with the SbSu gene recovered the sugary endosperm phenotype. Additionally, analyzing mutants obtained from an EMS-induced mutant panel revealed novel alleles with phenotypes showing less severe wrinkles and higher Brix scores. These results suggested that SbSu was the corresponding gene for the sugary endosperm. Expression profiles of starch synthesis genes during the grain-filling stage demonstrated that a loss-of-function of SbSu affects the expression of most starch synthesis genes and revealed the fine-tuned gene regulation in the starch synthetic pathway in sorghum. Haplotype analysis using 187 diverse accessions from a sorghum panel revealed the haplotype of SUF showing severe phenotype had not been used among the landraces and modern varieties. Thus, weak alleles (showing sweet and less severe wrinkles), such as in the abovementioned EMS-induced mutants, are more valuable for grain sorghum breeding. Our study suggests that more moderate alleles (e.g. produced by genome editing) should be beneficial for improving grain sorghum.

  Frontiers Media SA, Feb. 2023, Frontiers in Plant Science, 14, 1114935 - 1114935, English, International magazine

  Scientific journal


• Pretreatment with dilute maleic acid enhances the enzymatic digestibility of sugarcane bagasse and oil palm empty fruit bunch fiber

  Lucky Risanto, Deddy Triyono Nugroho Adi, Triyani Fajriutami, Hiroshi Teramura, Widya Fatriasari, Euis Hermiati, Prihardi Kahar, Akihiko Kondo, Chiaki Ogino

  Lignocellulose is resistant to degradation and requires pretreatment before hydrolytic enzymes can release fermentable sugars. Sulfuric acid has been widely used for biomass pretreatment, but high amount of degradation products usually occurred when using this method. To enhance accessibility to cellulose, we studied the performances of several dilute organic acid pretreatments of sugarcane bagasse and oil palm empty fruit bunch fiber. The results revealed that pretreatment with maleic acid yields the highest xylose and glucose release among other organic acids. The effects of concentration, duration of heating and heating temperature were further studied. Dilute maleic acid 1% (w/w) pretreatment at 180 °C was the key to its viability as a substitute for sulfuric acid. Moreover, maleic acid did not seem to highly promote the formation of either furfural or 5-HMF in the liquid hydrolysate after pretreatment.

  Elsevier BV, Feb. 2023, Bioresource Technology, 369, 128382 - 128382, English, International magazine

  [Refereed]

  Scientific journal


• Fluorophore‐Decorated Mie Resonant Silicon Nanosphere for Scattering/Fluorescence Dual‐Mode Imaging

  Masato Adachi, Hiroshi Sugimoto, Yuya Nishimura, Kenta Morita, Chiaki Ogino, Minoru Fujii

  Wiley, Jan. 2023, Small, 19(14) (14), 2207318 - 2207318, English, International magazine

  [Refereed]

  Scientific journal


• The Preparation of Nanoemulsion Formulation from Pelargonic Acid in Lignin Carrier and Its Application to Weeds

  Restu, W.K., Septiyanti, M., Triwulandari, E., Aryana, N., Sampora, Y., Devy, Y.A., Ghozali, M., Muryanto, Meliana, Y., Sondari, D., Fatriasari, W., Ogino, C.

  2023, Journal of the Institution of Engineers India Series E, 104(2) (2)

  Scientific journal


• Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant Escherichia coli

  Hideo Kawaguchi, Shunsuke Masuo, Keiko Wakai, Naoki Takaya, Tomohisa Hasunma, Tatsuo Kaneko, Satoshi Okada, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

  4-Aminophenylalanine as a diamine monomer to synthesize unique biopolyimides was produced from lignocellulosic biomass by microbial fermentation. Fermentation inhibitors present in lignocellulosic hydrolysate of sorghum bagasse were identified.

  Royal Society of Chemistry (RSC), 2023, RSC Sustainability

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Enhanced growth and lipid productivity by living Chlorella sorokiniana immobilized in Ca-alginate beads

  Alfaro-Sayes, D.A., Amoah, J., Rachmadona, N., Hama, S., Hasunuma, T., Kondo, A., Ogino, C.

  IOP Publishing, Jan. 2023, Jphys Energy, 5(1) (1), 014019 - 014019, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Alginate immobilization as a strategy for improving succinate production during autofermentation using cyanobacteria Synechocystis sp. PCC 6803

  Alfaro-Sayes, D.A., Amoah, J., Aikawa, S., Matsuda, M., Hasunuma, T., Kondo, A., Ogino, C.

  Elsevier BV, Dec. 2022, Biochemical Engineering Journal, 188, 108681 - 108681, English

  [Refereed]

  Scientific journal


• Comprehensive Machine Learning Prediction of Extensive Enzymatic Reactions

  Naoki Watanabe, Masaki Yamamoto, Masahiro Murata, Christopher J. Vavricka, Chiaki Ogino, Akihiko Kondo, Michihiro Araki

  Sep. 2022, Journal of Physical Chemistry B, 126(36) (36), 6762 - 6770, English, International magazine

  Scientific journal


• Comprehensive Machine Learning Prediction of Extensive Enzymatic Reactions.

  Naoki Watanabe, Masaki Yamamoto, Masahiro Murata, Christopher J Vavricka, Chiaki Ogino, Akihiko Kondo, Michihiro Araki

  New enzyme functions exist within the increasing number of unannotated protein sequences. Novel enzyme discovery is necessary to expand the pathways that can be accessed by metabolic engineering for the biosynthesis of functional compounds. Accordingly, various machine learning models have been developed to predict enzymatic reactions. However, the ability to predict unknown reactions that are not included in the training data has not been clarified. In order to cover uncertain and unknown reactions, a wider range of reaction types must be demonstrated by the models. Here, we establish 16 expanded enzymatic reaction prediction models developed using various machine learning algorithms, including deep neural network. Improvements in prediction performances over that of our previous study indicate that the updated methods are more effective for the prediction of enzymatic reactions. Overall, the deep neural network model trained with combined substrate-enzyme-product information exhibits the highest prediction accuracy with Macro F1 scores up to 0.966 and with robust prediction of unknown enzymatic reactions that are not included in the training data. This model can predict more extensive enzymatic reactions in comparison to previously reported models. This study will facilitate the discovery of new enzymes for the production of useful substances.

  Sep. 2022, The journal of physical chemistry. B, 126(36) (36), 6762 - 6770, English, International magazine

  Scientific journal


• Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization.

  Radityo Pangestu, Prihardi Kahar, Lutfi Nia Kholida, Urip Perwitasari, Ahmad Thontowi, Fahrurrozi, Puspita Lisdiyanti, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  Acidic and chemical inhibitor stresses undermine efficient lactic acid bioproduction from lignocellulosic feedstock. Requisite coping treatments, such as detoxification and neutralizing agent supplementation, can be eliminated if a strong microbial host is employed in the process. Here, we exploited an originally robust yeast, Saccharomyces cerevisiae BTCC3, as a production platform for lactic acid. This wild-type strain exhibited a rapid cell growth in the presence of various chemical inhibitors compared to laboratory and industrial strains, namely BY4741 and Ethanol-red. Pathway engineering was performed on the strain by introducing an exogenous LDH gene after disrupting the PDC1 and PDC5 genes. Facilitated by this engineered strain, high cell density cultivation could generate lactic acid with productivity at 4.80 and 3.68 g L-1 h-1 under semi-neutralized and non-neutralized conditions, respectively. Those values were relatively higher compared to other studies. Cultivation using real lignocellulosic hydrolysate was conducted to assess the performance of this engineered strain. Non-neutralized fermentation using non-detoxified hydrolysate from sugarcane bagasse as a medium could produce lactic acid at 1.69 g L-1 h-1, which was competitive to the results from other reports that still included detoxification and neutralization steps in their experiments. This strategy could make the overall lactic acid bioproduction process simpler, greener, and more cost-efficient.

  Springer Science and Business Media LLC, Aug. 2022, Scientific reports, 12(1) (1), 13645 - 13645, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The flocculant Saccharomyces cerevisiae strain gains robustness via alteration of the cell wall hydrophobicity

  Prihardi Kahar, Akiho Itomi, Hikari Tsuboi, Miki Ishizaki, Misa Yasuda, Chie Kihira, Hiromi Otsuka, Nurlina binti Azmi, Hana Matsumoto, Chiaki Ogino, Akihiko Kondo

  When lignocellulosic biomass is utilized as a fermentative substrate to produce biochemicals, the existence of a yeast strain resistant to inhibitory chemical compounds (ICCs) released from the biomass becomes critical. To achieve the purpose, in this study, Saccharomyces yeast strains from a NBRC yeast culture collection were used for exploration and evaluated in two different media containing ICCs that mimic one another but resemble the hydrolysate of real biomass. Among them, S. cerevisiae F118 strain shows robustness upon the fermentation with unique flocculation trait that was strongly responsive to ICC stress. When this strain was cultured in the presence of ICCs, its cell wall hydrophobicity increased dramatically, and reduced significantly when the ICCs were depleted, demonstrating that cell-surface hydrophobicity can also act as an adaptive response to the ICCs. Cells from the strain with the highest cell-wall hydrophobicity displayed progressively stronger flocculation, indicating that the F118 strain is having unique robustness under ICC stress. Gene expression perturbation analysis revealed that mot3 gene encoding regulatory Mot3p from the F118 strain was expressed in response to the concentration of ICCs. This gene was found to control expression of ygp1 gene that encoding Ygp1p, one of cell wall proteins. Deep sequencing analysis revealed that the Mot3p of the F118 strain features a unique insertion and deletion of nucleotides that encode glutamine or asparagine residues, particularly in N-terminal domain, as determined by comparison to the Mot3p sequence from the S288c strain, which was employed as a control strain. Furthermore, the cell wall hydrophobicity of the S288c strain was greatly enhanced and became ICC-responsive after gene swapping with the mot3 gene from the F118 strain. The gene-swapped S288c strain fermented 6-fold faster than the wild-type strain, producing 14.5 g/L of ethanol from 30 g/L of glucose consumed within 24 h in a medium containing the ICCs. These such modifications to Mot3p in unique locations in its sequence have a potential to change the expression of a gene involved in cell wall hydrophobicity and boosted the flocculation response to ICC stress, allowing for the acquisition of extraordinary robustness.

  Elsevier BV, Jul. 2022, Metabolic Engineering, 72, 82 - 96, English, International magazine

  Scientific journal


• Integrated bioconversion process for biodiesel production utilizing waste from the palm oil industry

  Rachmadona, N., Harada, Y., Amoah, J., Quayson, E., Aznury, M., Hama, S., Kondo, A., Ogino, C.

  Elsevier BV, Jun. 2022, Journal of Environmental Chemical Engineering, 10(3) (3), 107550 - 107550, English

  [Refereed]

  Scientific journal


• Reactive oxygen species-inducing titanium peroxide nanoparticles as promising radiosensitizers for eliminating pancreatic cancer stem cells

  Mohammed Salah, Hiroaki Akasaka, Yasuyuki Shimizu, Kenta Morita, Yuya Nishimura, Hikaru Kubota, Hiroki Kawaguchi, Tomomi Sogawa, Naritoshi Mukumoto, Chiaki Ogino, Ryohei Sasaki

  Apr. 2022, JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 41(1) (1), 146 - 146, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Manno-Oligosaccharide Production from Biomass Hydrolysis by Using Endo-1,4-beta-Mannanase (ManNj6-379) from Nonomuraea jabiensis ID06-379

  Shanti Ratnakomala, Prihardi Kahar, Norimasa Kashiwagi, JaeMin Lee, Motonori Kudou, Hana Matsumoto, Pamella Apriliana, Yopi Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

  Feb. 2022, PROCESSES, 10(2) (2), English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 3-Amino-4-hydroxybenzoic acid production from glucose and/or xylose via recombinant <i>Streptomyces lividans</i>

  Satoko Niimi-Nakamura, Hideo Kawaguchi, Kouji Uematsu, Hiroshi Teramura, Sachiko Nakamura-Tsuruta, Norimasa Kashiwagi, Yoshinori Sugai, Yohei Katsuyama, Yasuo Ohnishi, Chiaki Ogino, Akihiko Kondo

  The aromatic compound 3-amino-4-hydroxybenzoic acid (3,4-AHBA) can be employed as a raw material for high-performance industrial plastics. The aim of this study is to produce 3,4-AHBA via a recombinant Streptomyces lividans strain containing griI and griH genes derived from Streptomyces griseus using culture medium with glucose and/or xylose, which are the main components in lignocellulosic biomass. Production of 3,4-AHBA by the recombinant S. lividans strain was successful, and the productivity was affected by the kind of sugar used as an additional carbon source. Metabolic profiles revealed that L aspartate-4-semialdehyde (ASA), a precursor of 3,4-AHBA, and coenzyme NADPH were supplied in greater amounts in xylose medium than in glucose medium. Moreover, cultivation in TSB medium with a mixed sugar (glucose/xylose) was found to be effective for 3,4-AHBA production, and optimal conditions for efficient production were designed by changing the ratio of glucose to xylose. The best productivity of 2.70 g/L was achieved using a sugar mixture of 25 g/L glucose and 25 g/L xylose, which was 1.5 times higher than the result using 50 g/L glucose alone. These results suggest that Streptomyces is a suitable candidate platform for 3,4-AHBA production from lignocellulosic biomass-derived sugars under appropriate culture conditions.

  Microbiology Research Foundation, 2022, The Journal of General and Applied Microbiology, 68(2) (2), 109 - 116, English, Domestic magazine

  [Refereed]

  Scientific journal


• An integrated biorefinery strategy for the utilization of palm-oil wastes

  Prihardi Kahar, Nova Rachmadona, Radityo Pangestu, Rendi Palar, Deddy Triyono Nugroho Adi, Ario Betha Juanssilfero, Yopi, Immanuel Manurung, Shinji Hama, Chiaki Ogino

  Each year, the palm oil industry generates a significant amount of biomass residue and effluent waste; both have been identified as significant sources of greenhouse gas (GHG) emissions. This issue poses a severe environmental challenge for the industry due to the possibility of long-term negative effects on human well-being. The palm-oil industry must invest significantly in the technology that is required to resolve these issues and to increase the industry's sustainability. However, current technologies for converting wastes such as lignocellulosic components and effluents into biochemical products are insufficient for optimal utilization. This review discusses the geographical availability of palm-oil biomass, its current utilization routes, and then recommends the development of technology for converting palm-oil biomass into value-added products through an integrated biorefinery strategy. Additionally, this review summarizes the palm oil industry's contribution to achieving sustainable development goals (SDGs) through a circular bioeconomy concept.

  Elsevier BV, Jan. 2022, Bioresource Technology, 344(Pt B) (Pt B), 126266 - 126266, English, International magazine

  Scientific journal


• Recent advances in lignocellulosic biomass white biotechnology for bioplastics

  Hideo Kawaguchi, Kenji Takada, Taghreed Elkasaby, Radityo Pangestu, Masakazu Toyoshima, Prihardi Kahar, Chiaki Ogino, Tatsuo Kaneko, Akihiko Kondo

  Lignocellulosic biomass has great potential as an inedible feedstock for bioplastic synthesis, although its use is still limited compared to current edible feedstocks of glucose and starch. This review focuses on recent advances in the production of biopolymers and biomonomers from lignocellulosic feedstocks with downstream processing and chemical polymer syntheses. In microbial production, four routes composed of existing poly (lactic acid) and polyhydroxyalkanoates (PHAs) and the emerging biomonomers of itaconic acid and aromatic compounds were presented to review present challenges and future perspectives, focusing on the use of lignocellulosic feedstocks. Recently, advances in purification technologies decreased the number of processes and their environmental burden. Additionally, the unique structures and high-performance of emerging lignocellulose-based bioplastics have expanded the possibilities for the use of bioplastics. The sequence of processes provides insight into the emerging technologies that are needed for the practical use of bioplastics made from lignocellulosic biomass.

  Elsevier BV, Jan. 2022, Bioresource Technology, 344(Pt B) (Pt B), 126165 - 126165, English, International magazine

  Scientific journal


• Enhanced production of γ-amino acid 3-amino-4-hydroxybenzoic acid by recombinant Corynebacterium glutamicum under oxygen limitation

  Hideo Kawaguchi, Tomohisa Hasunuma, Yasuo Ohnishi, Takashi Sazuka, Akihiko Kondo, Chiaki Ogino

  Bio-based aromatic compounds are of great interest to the industry, as commercial production of aromatic compounds depends exclusively on the unsustainable use of fossil resources or extraction from plant resources. γ-amino acid 3-amino-4-hydroxybenzoic acid (3,4-AHBA) serves as a precursor for thermostable bioplastics. Under aerobic conditions, a recombinant Corynebacterium glutamicum strain KT01 expressing griH and griI genes derived from Streptomyces griseus produced 3,4-AHBA with large amounts of amino acids as by-products. The specific productivity of 3,4-AHBA increased with decreasing levels of dissolved oxygen (DO) and was eightfold higher under oxygen limitation (DO = 0 ppm) than under aerobic conditions (DO ≥ 2.6 ppm). Metabolic profiles during 3,4-AHBA production were compared at three different DO levels (0, 2.6, and 5.3 ppm) using the DO-stat method. Results of the metabolome analysis revealed metabolic shifts in both the central metabolic pathway and amino acid metabolism at a DO of < 33% saturated oxygen. Based on this metabolome analysis, metabolic pathways were rationally designed for oxygen limitation. An ldh deletion mutant, with the loss of lactate dehydrogenase, exhibited 3.7-fold higher specific productivity of 3,4-AHBA at DO = 0 ppm as compared to the parent strain KT01 and produced 5.6 g/L 3,4-AHBA in a glucose fed-batch culture. Our results revealed changes in the metabolic state in response to DO concentration and provided insights into oxygen supply during fermentation and the rational design of metabolic pathways for improved production of related amino acids and their derivatives.

  Springer Science and Business Media LLC, Dec. 2021, Microbial Cell Factories, 20(1) (1), 228 - 228, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Accelerated glucose metabolism in hyphae-dispersed Aspergillus oryzae is suitable for biological production

  Taku Sakuragawa, Satoshi Wakai, Silai Zhang, Hideo Kawaguchi, Chiaki Ogino, Akihiko Kondo

  Recently, a hyphae-dispersed type of filamentous fungus Aspergillus oryzae was constructed via genetic engineering, and industrial applications are expected due to the ease of handling and to the level of protein production properties. In this study, we constructed cellulase-expressing strains using wild-type and hyphae-dispersed strains to investigate the correlation between protein productivity and metabolism. Compared with the original strain, the hyphae-dispersed cellulase-expressing strain showed elevated cellulase activity, rapid glucose consumption, increased mycelial dry weight, an increased expression of cellulase genes, and activated respiration activity. Comparative metabolomic analysis showed fewer metabolites in the glycolysis and TCA cycles in the dispersed strains than in the original strains. These results indicate that the flux of carbohydrate metabolism in the hyphae-dispersed strains is smoother than that in the original strains. Such efficient metabolic flux would contribute to efficient energy conversion and to sufficient energy supply to anabolisms, such as mycelial growth and protein production. Our findings suggest that the hyphae-dispersed strains could be a useful host not only for protein production but also for the biological production of various chemicals such as organic acids.

  Elsevier BV, Aug. 2021, Journal of Bioscience and Bioengineering, 132(2) (2), 140 - 147, English, Domestic magazine

  Scientific journal


• Utilizing palm oil mill effluent (POME) for the immobilization of Aspergillus oryzae whole-cell lipase strains for biodiesel synthesis

  Rachmadona, N., Quayson, E., Amoah, J., Alfaro-Sayes, D.A., Hama, S., Aznury, M., Kondo, A., Ogino, C.

  Wiley, May 2021, Biofuels Bioproducts and Biorefining, 15(3) (3), 804 - 814, English

  Scientific journal


• ウサギVX2腫瘍モデルを用いた過酸化チタンナノ粒子動注の基礎的研究

  岡田 卓也, 元津 倫幸, 西村 勇哉, 森田 健太, 上嶋 英介, 山口 雅人, 杉本 幸司, 佐々木 良平, 荻野 千秋, 村上 卓道

  (一社)日本インターベンショナルラジオロジー学会, Apr. 2021, 日本インターベンショナルラジオロジー学会雑誌, 36(Suppl.) (Suppl.), 191 - 191, Japanese


• Titanium oxide nano-radiosensitizers for hydrogen peroxide delivery into cancer cells

  Kenta Morita, Yuya Nishimura, Satoko Nakamura, Yuki Arai, Chiya Numako, Kazuyoshi Sato, Masao Nakayama, Hiroaki Akasaka, Ryohei Sasaki, Chiaki Ogino, Akihiko Kondo

  Polyacrylic acid-modified titanium peroxide nanoparticles (PAA-TiOx NPs) are promising radiosensitizers that enhance the therapeutic effect of X-ray irradiation after local injection into tumors. However, the mechanism for this reaction has remained unclear with the exception of the involvement of hydrogen peroxide (H2O2), which is released by PAA-TiOx NPs to a liquid phase during dispersion. In the present study, a clonogenic assay was used to compare PAA-TiOx NPs with free H2O2 molecules to investigate the effect exerted on the radiosensitivity of cancer cells in vitro. A cell-free dialysis method revealed that a portion of the H2O2 adsorbed onto the PAA-TiOx NPs during synthesis could be released during a treatment regimen. The H2O2 release lasted for 7 h, which was sufficient for one radiation treatment procedure. For in vitro experiments, cultured human pancreatic cancer cells took up PAA-TiOx NPs in 10 min after administration. Interestingly, when the cells were washed with a buffer after treatment with either a PAA-TiOx NP or H2O2 solution, the intracellular H2O2 levels remained higher with PAA-TiOx NP treatment compared with the H2O2 solution treatment. Furthermore, the effects of subsequent X-ray irradiation corresponded to the intracellular H2O2 levels. These results indicate that PAA-TiOx NPs are efficient carriers of H2O2 into cancer cells and thus enhance the radiosensitivity.

  Elsevier BV, Feb. 2021, Colloids and Surfaces B: Biointerfaces, 198, 111451 - 111451, English, International magazine

  Scientific journal


• Constitutive cell surface expression of ZZ domain for the easy preparation of yeast-based immunosorbents

  Kohei Katsurada, Masahiro Tominaga, Misato Kaishima, Hiroko Kato, Toshihide Matsuno, Chiaki Ogino, Akihiko Kondo, Jun Ishii, Katsumi Takayama

  We describe a novel expression cassette that enables efficient and constitutive expression of the ZZ domain derived from Staphylococcus aureus protein A on the yeast cell surface to easily prepare yeast-based immunosorbents. Using this expression cassette containing the PGK1 promoter, a secretion signal derived from α-factor, and a Flo1-derived anchor protein, we successfully created a yeast-based immunosorbent for human serum albumin.

  Microbiology Research Foundation, 2021, The Journal of General and Applied Microbiology, 67(6) (6), 265 - 268, English, Domestic magazine

  Scientific journal


• Ultrahigh Thermoresistant Lightweight Bioplastics Developed from Fermentation Products of Cellulosic Feedstock

  Aniruddha Nag, Mohammad Asif Ali, Hideo Kawaguchi, Shun Saito, Yukie Kawasaki, Shoko Miyazaki, Hirotoshi Kawamoto, Deddy Triyono Nugroho Adi, Kumiko Yoshihara, Shunsuke Masuo, Yohei Katsuyama, Akihiko Kondo, Chiaki Ogino, Naoki Takaya, Tatsuo Kaneko, Yasuo Ohnishi

  Wiley, Jan. 2021, Advanced Sustainable Systems, 5(1) (1), 2000193 - 2000193

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Stable near-infrared photoluminescence from silicon quantum dot-bovine serum albumin composites

  Inoue, A., Sugimoto, H., Sugimoto, Y., Akamatsu, K., Hubalek Kalbacova, M., Ogino, C., Fujii, M.

  Cambridge University Press ({CUP}), Dec. 2020, MRS Communications, 10(4) (4), 680 - 686

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Immobilized lipases for biodiesel production: Current and future greening opportunities

  Emmanuel Quayson, Jerome Amoah, Shinji Hama, Akihiko Kondo, Chiaki Ogino

  Elsevier BV, Dec. 2020, Renewable and Sustainable Energy Reviews, 134, 110355 - 110355

  Scientific journal


• In Vivo Evaluation of the ZHER2-BNC/LP Carrier Encapsulating an Anticancer Drug and a Radiosensitizer

  Yuya Nishimura, Ryosuke Ezawa, Kenta Morita, Masao Nakayama, Jun Ishii, Ryohei Sasaki, Chiaki Ogino, Akihiko Kondo

  American Chemical Society (ACS), Nov. 2020, ACS Applied Bio Materials, 3(11) (11), 7743 - 7751

  Scientific journal


• Valorization of palm biomass waste into carbon matrices for the immobilization of recombinant Fusarium heterosporum lipase towards palm biodiesel synthesis

  Emmanuel Quayson, Jerome Amoah, Nova Rachmadona, Kenta Morita, Lawrence Darkwah, Shinji Hama, Ayumi Yoshida, Akihiko Kondo, Chiaki Ogino

  Nov. 2020, Biomass and Bioenergy, 142, 105768 - 105768, English

  [Refereed]

  Scientific journal


• Pyruvate metabolism redirection for biological production of commodity chemicals in aerobic fungus Aspergillus oryzae.

  Silai Zhang, Satoshi Wakai, Naoya Sasakura, Hiroko Tsutsumi, Yoji Hata, Chiaki Ogino, Akihiko Kondo

  Pyruvate is a central metabolite for the biological production of various chemicals. In eukaryotes, pyruvate produced by glycolysis is used in conversion to ethanol and lactate and in anabolic metabolism in the cytosol, or is transported into the mitochondria for use as a substrate in the tricarboxylic acid (TCA) cycle. In this study, we focused on controlling pyruvate metabolism in aerobic microorganisms for the biological production of various chemicals. We successfully improved productivity by redirecting pyruvate metabolism in the aerobic filamentous fungus Aspergillus oryzae via the deletion of two genes that encode pyruvate decarboxylase and mitochondrial pyruvate carriers. Production of ethanol as a major byproduct was completely inhibited, and the limited translocation of pyruvate into the mitochondria shifted the metabolism from respiration for energy conversion to the effective production of lactate or 2,3-butandiole, even under aerobic conditions. Metabolomic and transcriptomic analyses showed an emphasis on glycolysis and a repressed TCA cycle. Although the dry mycelial weights of the deletion mutants were reduced compared with those of wild type, the titer and yields of the target products were drastically increased. In particular, the redirection of pyruvate metabolism shifted from anabolism for biomass production to catabolism for the production of target chemicals. Conclusively, our results indicate that the redirection of pyruvate metabolism is a useful strategy in the metabolic engineering of aerobic microorganisms.

  Jul. 2020, Metabolic engineering, English, International magazine

  [Refereed]

  Scientific journal


• A Comparative Assessment of Mechanisms and Effectiveness of Radiosensitization by Titanium Peroxide and Gold Nanoparticles.

  Mennaallah Hassan, Masao Nakayama, Mohammed Salah, Hiroaki Akasaka, Hikaru Kubota, Makiko Nakahana, Tatsuichiro Tagawa, Kenta Morita, Ai Nakaoka, Takeaki Ishihara, Daisuke Miyawaki, Kenji Yoshida, Yuya Nishimura, Chiaki Ogino, Ryohei Sasaki

  The development of potentially safe radiosensitizing agents is essential to enhance the treatment outcomes of radioresistant cancers. The titanium peroxide nanoparticle (TiOxNP) was originally produced using the titanium dioxide nanoparticle, and it showed excellent reactive oxygen species (ROS) generation in response to ionizing radiation. Surface coating the TiOxNPs with polyacrylic acid (PAA) showed low toxicity to the living body and excellent radiosensitizing effect on cancer cells. Herein, we evaluated the mechanism of radiosensitization by PAA-TiOxNPs in comparison with gold nanoparticles (AuNPs) which represent high-atomic-number nanoparticles that show a radiosensitizing effect through the emission of secondary electrons. The anticancer effects of both nanoparticles were compared by induction of apoptosis, colony-forming assay, and the inhibition of tumor growth. PAA-TiOxNPs showed a significantly more radiosensitizing effect than that of AuNPs. A comparison of the types and amounts of ROS generated showed that hydrogen peroxide generation by PAA-TiOxNPs was the major factor that contributed to the nanoparticle radiosensitization. Importantly, PAA-TiOxNPs were generally nontoxic to healthy mice and caused no histological abnormalities in the liver, kidney, lung, and heart tissues.

  Jun. 2020, Nanomaterials (Basel, Switzerland), 10(6) (6), English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• High enzymatic recovery and purification of xylooligosaccharides from empty fruit bunch via nanofiltration

  Hans Wijaya, Kengo Sasaki, Prihardi Kahar, Nanik Rahmani, Euis Hermiati, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  May 2020, Processes, 8(5) (5), 619, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Concentration of Lipase from Aspergillus oryzae Expressing Fusarium heterosporum by Nanofiltration to Enhance Transesterification

  Hans Wijaya, Kengo Sasaki, Prihardi Kahar, Emmanuel Quayson, Nova Rachmadona, Jerome Amoah, Shinji Hama, Chiaki Ogino, Akihiko Kondo

  Apr. 2020, Processes, 8(4) (4), 450, English

  [Refereed]

  Link to Kobe Univ. Repository (Kernel)


• Exploration and Evaluation of Machine Learning-Based Models for Predicting Enzymatic Reactions.

  Naoki Watanabe, Masahiro Murata, Teppei Ogawa, Christopher J Vavricka, Akihiko Kondo, Chiaki Ogino, Michihiro Araki

  Unannotated gene sequences in databases are increasing due to sequencing advances. Therefore, computational methods to predict functions of unannotated genes are needed. Moreover, novel enzyme discovery for metabolic engineering applications further encourages annotation of sequences. Here, enzyme functions are predicted using two general approaches, each including several machine learning algorithms. First, Enzyme-models (E-models) predict Enzyme Commission (EC) numbers from amino acid sequence information. Second, Substrate-Enzyme models (SE-models) are built to predict substrates of enzymatic reactions together with EC numbers, and Substrate-Enzyme-Product models (SEP-models) are built to predict substrates, products, and EC numbers. While accuracy of E-models is not optimal, SE-models and SEP-models predict EC numbers and reactions with high accuracy using all tested machine learning-based methods. For example, a single Random Forests-based SEP-model predicts EC first digits with an Average AUC score of over 0.94. Various metrics indicate that the current strategy of combining sequence and chemical structure information is effective at improving enzyme reaction prediction.

  Mar. 2020, Journal of chemical information and modeling, 60(3) (3), 1833 - 1843, English, International magazine

  [Refereed]

  Scientific journal


• Lipase-catalyzed ethanolysis for biodiesel production of untreated palm oil mill effluent

  Nova Rachmadona, Jerome Amoah, Emmanuel Quayson, Shinji Hama, Ayumi Yoshida, Akihiko Kondo, Chiaki Ogino

  Mar. 2020, SUSTAINABLE ENERGY & FUELS, 4(3) (3), 1105 - 1111, English

  [Refereed]

  Scientific journal


• Biodiesel-mediated biodiesel production: A recombinant Fusarium heterosporum lipase-catalyzed transesterification of crude plant oils

  Quayson, E., Amoah, J., Rachmadona, N., Hama, S., Yoshida, A., Kondo, A., Ogino, C.

  Mar. 2020, Fuel Processing Technology, 199, 106278, English

  [Refereed]

  Scientific journal


• Bio-processing of algal bio-refinery: a review on current advances and future perspectives.

  Apurav Krishna Koyande, Pau-Loke Show, Ruixin Guo, Bencan Tang, Chiaki Ogino, Jo-Shu Chang

  Microalgae biomass contains various useful bio-active components. Microalgae derived biodiesel has been researched for almost two decades. However, sole biodiesel extraction from microalgae is time-consuming and is not economically feasible due to competitive fossil fuel prices. Microalgae also contains proteins and carbohydrates in abundance. Microalgae are likewise utilized to extract high-value products such as pigments, anti-oxidants and long-chain polyunsaturated fatty acids which are useful in cosmetic, pharmaceutical and nutraceutical industry. These compounds can be extracted simultaneously or sequentially after biodiesel extraction to reduce the total expenditure involved in the process. This approach of bio-refinery is necessary to promote microalgae in the commercial market. Researchers have been keen on utilizing the bio-refinery approach to exploit the valuable components encased by microalgae. Apart from all the beneficial components housed by microalgae, they also help in reducing the anthropogenic CO2 levels of the atmosphere while utilizing saline or wastewater. These benefits enable microalgae as a potential source for bio-refinery approach. Although life-cycle analysis and economic assessment do not favor the use of microalgae biomass feedstock to produce biofuel and co-products with the existing techniques, this review still aims to highlight the beneficial components of microalgae and their importance to humans. In addition, this article also focuses on current and future aspects of improving the feasibility of bio-processing for microalgae bio-refinery.

  Dec. 2019, Bioengineered, 10(1) (1), 574 - 592, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• High cell density cultivation of Lipomyces starkeyi for achieving highly efficient lipid production from sugar under low C/N ratio

  Amza Rezky Lastinov, Kahar Prihardi, Juanssilfero Ario Betha, Miyamoto Nao, Otsuka Hiromi, Kihira Chie, Ogino Chiaki, Kondo Akihiko

  Sep. 2019, BIOCHEMICAL ENGINEERING JOURNAL, 149

  [Refereed]


• Co-fermentation of xylose and glucose from ionic liquid pretreated sugar cane bagasse for bioethanol production using engineered xylose assimilating yeast

  Amoah, J., Ogura, K., Schmetz, Q., Kondo, A., Ogino, C.

  Sep. 2019, Biomass and Bioenergy, 128

  [Refereed]

  Scientific journal


• Combined Cell Surface Display of β-d-Glucosidase (BGL), Maltose Transporter (MAL11), and Overexpression of Cytosolic Xylose Reductase (XR) in Saccharomyces cerevisiae Enhance Cellobiose/Xylose Coutilization for Xylitol Bioproduction from Lignocellulosic Biomass.

  Gregory G Y Guirimand, Takahiro Bamba, Mami Matsuda, Kentaro Inokuma, Kenta Morita, Yuki Kitada, Yuma Kobayashi, Takahiro Yukawa, Kengo Sasaki, Chiaki Ogino, Tomohisa Hasunuma, Akihiko Kondo

  Xylitol is a highly valuable commodity chemical used extensively in the food and pharmaceutical industries. The production of xylitol from d-xylose involves a costly and polluting catalytic hydrogenation process. Biotechnological production from lignocellulosic biomass by micro-organisms like yeasts is a promising option. In this study, xylitol is produced from lignocellulosic biomass by a recombinant strain of Saccharomyces cerevisiae (S. cerevisiae) (YPH499-SsXR-AaBGL) expressing cytosolic xylose reductase (Scheffersomyces stipitis xylose reductase [SsXR]), along with a β-d-glucosidase (Aspergillus aculeatus β-glucosidase 1 [AaBGL]) displayed on the cell surface. The simultaneous cofermentation of cellobiose/xylose by this strain leads to an ≈2.5-fold increase in Yxylitol/xylose (=0.54) compared to the use of a glucose/xylose mixture as a substrate. Further improvement in the xylose uptake by the cell is achieved by a broad evaluation of several homologous and heterologous transporters. Homologous maltose transporter (ScMAL11) shows the best performance in xylose transport and is used to generate the strain YPH499-XR-ScMAL11-BGL with a significantly improved xylitol production capacity from cellobiose/xylose coutilization. This report constitutes a promising proof of concept to further scale up the biorefinery industrial production of xylitol from lignocellulose by combining cell surface and metabolic engineering in S. cerevisiae.

  Sep. 2019, Biotechnology journal, 14(9) (9), e1800704, English, International magazine

  [Refereed]

  Scientific journal


• Author Correction: Building a global alliance of biofoundries.

  Nathan Hillson, Mark Caddick, Yizhi Cai, Jose A Carrasco, Matthew Wook Chang, Natalie C Curach, David J Bell, Rosalind Le Feuvre, Douglas C Friedman, Xiongfei Fu, Nicholas D Gold, Markus J Herrgård, Maciej B Holowko, James R Johnson, Richard A Johnson, Jay D Keasling, Richard I Kitney, Akihiko Kondo, Chenli Liu, Vincent J J Martin, Filippo Menolascina, Chiaki Ogino, Nicola J Patron, Marilene Pavan, Chueh Loo Poh, Isak S Pretorius, Susan J Rosser, Nigel S Scrutton, Marko Storch, Hille Tekotte, Evelyn Travnik, Claudia E Vickers, Wen Shan Yew, Yingjin Yuan, Huimin Zhao, Paul S Freemont

  Jul. 2019, Nature communications, 10(1) (1), 3132 - 3132, English, International magazine

  [Refereed]

  Link to Kobe Univ. Repository (Kernel)


• Versatility of a Dilute Acid/Butanol Pretreatment Investigated on Various Lignocellulosic Biomasses to Produce Lignin, Monosaccharides and Cellulose in Distinct Phases

  Quentin Schmetz, Hiroshi Teramura, Kenta Morita, Tomoko Oshima, Aurore Richel, Chiaki Ogino, Akihiko Kondo

  Jul. 2019, ACS Sustainable Chemistry and Engineering, 7(13) (13), 11069 - 11079

  [Refereed]

  Scientific journal


• Emerging crosslinking techniques for glove manufacturers with improved nitrile glove properties and reduced allergic risks

  Yew, G.Y., Tham, T.C., Law, C.L., Chu, D.-T., Ogino, C., Show, P.L.

  Jun. 2019, Materials Today Communications, 19, 39 - 50

  [Refereed]

  Scientific journal


• Enhanced Phenyllactic Acid Production in Escherichia coli Via Oxygen Limitation and Shikimate Pathway Gene Expression.

  Hideo Kawaguchi, Hiroki Miyagawa, Sachiko Nakamura-Tsuruta, Naoki Takaya, Chiaki Ogino, Akihiko Kondo

  3-Phenyllactic acid (PhLA) is useful as a start-up material in the pharmaceutical and biorefinery industries. To enhance the production of PhLA from glucose using recombinant Escherichia coli, the effects of glucose concentration and oxygen limitation on PhLA production are assessed in a fed-batch system using dissolved oxygen (DO)-stat method. The highest titer of PhLA (7.3 g L-1 ) is observed with a high concentration of glucose and under oxygen-limited conditions (DO = 0 ppm). Under oxygen limitation, cell growth and the formation of acetate and l-phenylalanine (Phe) by-products after 72 h of cultivation are reduced by 30%, 70%, and 81%, respectively, as compared to that under high DO conditions (DO = 5 ppm). Gene expression levels are compared between low and high DO conditions by quantitative polymerase chain reaction (qPCR) analysis. Several genes in the glycolysis (gapA and pykA), pentose phosphate (tktA), and early shikimate pathways for PhLA biosynthesis (aroF, aroG, and aroH) are upregulated under oxygen limitation. The results suggest that oxygen limitation affects metabolism in the shikimate pathway at both metabolic and transcriptional levels and that controlling the DO level is critical for enhanced production of a variety of aromatic compounds through the shikimate pathway.

  Jun. 2019, Biotechnology journal, 14(6) (6), e1800478, English, International magazine

  [Refereed]

  Scientific journal


• Bioenergy and Biorefinery: Feedstock, Biotechnological Conversion, and Products.

  Jerome Amoah, Prihardi Kahar, Chiaki Ogino, Akihiko Kondo

  Biorefinery has been suggested to provide relevant substitutes to a number of fossil products. Feedstocks and conversion technologies have, however, been the bottleneck to the realization of this concept. Herein, feedstocks and bioconversion technologies under biorefinery have been reviewed. Over the last decade, research has shown possibilities of generating tens of new products but only few industrial implementations. This is partly associated with low production yields and poor cost-competitiveness. This review addresses the technical barriers associated with the conversion of emerging feedstocks into chemicals and bioenergy platforms and summarizes the developed biotechnological approaches including advances in metabolic engineering. This summary further suggests possible future advances that would expand the portfolio of biorefinery and speed up the realization of biofuels and biochemicals.

  Jun. 2019, Biotechnology journal, 14(6) (6), e1800494, English, International magazine

  [Refereed]

  Scientific journal


• Lipid production by Lipomyces starkeyi using sap squeezed from felled old oil palm trunks.

  Ario Betha Juanssilfero, Prihardi Kahar, Rezky Lastinov Amza, Yopi, Kumar Sudesh, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  Jun. 2019, Journal of bioscience and bioengineering, 127(6) (6), 726 - 731, English, Domestic magazine

  [Refereed]

  Scientific journal


• Building a global alliance of biofoundries.

  Nathan Hillson, Mark Caddick, Yizhi Cai, Jose A Carrasco, Matthew Wook Chang, Natalie C Curach, David J Bell, Rosalind Le Feuvre, Douglas C Friedman, Xiongfei Fu, Nicholas D Gold, Markus J Herrgård, Maciej B Holowko, James R Johnson, Richard A Johnson, Jay D Keasling, Richard I Kitney, Akihiko Kondo, Chenli Liu, Vincent J J Martin, Filippo Menolascina, Chiaki Ogino, Nicola J Patron, Marilene Pavan, Chueh Loo Poh, Isak S Pretorius, Susan J Rosser, Nigel S Scrutton, Marko Storch, Hille Tekotte, Evelyn Travnik, Claudia E Vickers, Wen Shan Yew, Yingjin Yuan, Huimin Zhao, Paul S Freemont

  May 2019, Nature communications, 10(1) (1), 2040 - 2040, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Cell-surface display technology and metabolic engineering of Saccharomyces cerevisiae for enhancing xylitol production from woody biomass

  Guirimand Gregory, Inokuma Kentaro, Bamba Takahiro, Matsuda Mami, Morita Kenta, Sasaki Kengo, Ogino Chiaki, Berrin Jean-Guy, Hasunuma Tomohisa, Kondo Akihiko

  Apr. 2019, GREEN CHEMISTRY, 21(7) (7), 1795 - 1808, English

  [Refereed]

  Scientific journal


• Valorization of Activated Carbon as a Reusable Matrix for the Immobilization of Aspergillus oryzae Whole-Cells Expressing Fusarium heterosporum Lipase toward Biodiesel Synthesis

  Quayson Emmanuel, Amoah Jerome, Hama Shinji, Yoshida Ayumi, Morita Kenta, Kondo Akihiko, Ogino Chiaki

  Mar. 2019, ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 7(5) (5), 5010 - 5017, English

  [Refereed]

  Scientific journal


• Modified expression of multi-cellulases in a filamentous fungus Aspergillus oryzae.

  Satoshi Wakai, Nanami Nakashima, Chiaki Ogino, Hiroko Tsutsumi, Yoji Hata, Akihiko Kondo

  Aspergillus oryzae, a filamentous fungus, can secrete large amounts of enzymes extracellularly. We constructed a genetically engineered A. oryzae that simultaneously produced cellobiohydrolase, endoglucanase, and β-glucosidase by integrating multiple copies of the genes encoding these cellulases into fungal chromosomes. The resulting strain possessed 5-16 copies of each cellulase gene within the chromosome and showed approximately 10-fold higher activity versus single integration strains. Copy number polymorphisms were attributed to differences in flanking region sequence for the integrated gene fragments. Furthermore, we found that the P-sodM/T-glaB set demonstrated the strongest transcription levels per gene copy number. We therefore modified promoter/terminator set and cellulase gene combinations based on this polymorphism and transcription level data, with the resulting transformant showing 40-fold higher cellulolytic activity versus the single integration strain. This designed expression method could be useful for the overexpression of multiple enzymes and pathway flux control-mediated metabolic engineering in A. oryzae.

  Mar. 2019, Bioresource technology, 276, 146 - 153, English, International magazine

  [Refereed]

  Scientific journal


• Efficient and Supplementary Enzyme Cocktail from Actinobacteria and Plant Biomass Induction.

  Musashi Takenaka, Jae M Lee, Prihardi Kahar, Chiaki Ogino, Akihiko Kondo

  Actinobacteria plays a key role in the cycling of organic matter in soils. They secret biomass-degrading enzymes that allow it to produce the unique metabolites that originate in plant biomass. Although past studies have focused on these unique metabolites, a large-scale screening of Actinobacteria is yet to be reported to focus on their biomass-degrading ability. In the present study, a rapid and simple method is constructed for a large-scale screening, and the novel resources that form the plant biomass-degrading enzyme cocktail are identified from 850 isolates of Actinobacteria. As a result, Nonomuraea fastidiosa secretes a biomass degrading enzyme cocktail with the highest enzyme titer, although cellulase activities are lower than a commercially available enzyme. So the rich accessory enzymes are suggested to contribute to the high enzyme titer for a pretreated bagasse with a synergistic effect. Additionally, an optimized cultivation method of biomass induction caused to produce the improved enzyme cocktail indicated strong enzyme titers and a strong synergistic effect. Therefore, the novel enzyme cocktails are selected via the optimized method for large-scale screening, and then the enzyme cocktail can be improved via the optimized production with biomass-induction.

  Mar. 2019, Biotechnology journal, 14(3) (3), e1700744, English, International magazine

  [Refereed]

  Scientific journal


• 5-Hydroxymethylfurfural production from salt-induced photoautotrophically cultivated Chlorella sorokiniana

  Amoah, J., Hasunuma, T., Ogino, C., Kondo, A.

  Feb. 2019, Biochemical Engineering Journal, 142, 117 - 123, English

  [Refereed]

  Scientific journal


• GH-10 and GH-11 Endo-1,4-β-xylanase enzymes from Kitasatospora sp. produce xylose and xylooligosaccharides from sugarcane bagasse with no xylose inhibition.

  Nanik Rahmani, Prihardi Kahar, Puspita Lisdiyanti, Jaemin Lee, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

  A novel strategy for the low-cost, high-yield co-production of xylose and xylooligosaccharides together with no xylose inhibition was developed using a novel heterologous expression of XYN10Ks_480 endo-1,4-β-xylanase with a ricin-type β-trefoil type of domain and XYN11Ks_480 endo-1,4-β-xylanase with a CBM 2 superfamily from the Kitasatospora sp in an actinomycetes expression system. Xylose is the main building block for hemicellulose xylan. Our findings demonstrated high levels of expression and catalytic activity for XYN10Ks_480 during hydrolysis of the extracted xylan of bagasse, and three types of xylan-based substrates were used to produce xylose and xylooligosaccharides. However, hydrolysis by XYN11Ks_480 produced xylooligosaccharides without xylose formation. This study demonstrated how integrating sodium hypochlorite-extracted xylan and enzymatic hydrolysis could provide an alternative strategy for the generation of XOS from lignocellulosic material.

  Jan. 2019, Bioresource technology, 272, 315 - 325, English, International magazine

  [Refereed]

  Scientific journal


• Investigation of the potential of using TiO2 nanoparticles as a contrast agent in computed tomography and magnetic resonance imaging

  Hiroaki Akasaka, Naritoshi Mukumoto, Masao Nakayama, Tianyuan Wang, Ryuichi Yada, Yasuyuki Shimizu, Sachiko Inubushi, Katsusuke Kyotani, Keisuke Okumura, Masanori Miyamoto, Ai Nakaoka, Kenta Morita, Yuya Nishimura, Chiaki Ogino, Ryohei Sasaki

  2019, Applied Nanoscience (Switzerland), 1 - 6, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Mechanism of the Fe-Assisted Hydrothermal Liquefaction of Lignocellulosic Biomass

  Yoshinori Miyata, Kunimasa Sagata, Yoshiko Yamazaki, Hiroshi Teramura, Yoshiaki Hirano, Chiaki Ogino, Yuichi Kita

  American Chemical Society, Nov. 2018, Industrial and Engineering Chemistry Research, 57(44) (44), 14870 - 14877, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Repeated ethanol fermentation from membrane-concentrated sweet sorghum juice using the flocculating yeast Saccharomyces cerevisiae F118 strain.

  Hans Wijaya, Kengo Sasaki, Prihardi Kahar, Yopi, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  The aim of this study was to construct a cost-effective method for repeated bioethanol production using membrane (ultrafiltration permeation and nanofiltration concentration)-concentrated sweet sorghum juice by using flocculent Saccharomyces cerevisiae F118 strain. With low initial dry cell concentrations at around 0.28-0.35 g L-1, the S. cerevisiae F118 strain provided an ethanol titer of 86.19 ± 1.15 g L-1 (theoretical ethanol yield of 70.77%), which was higher than the non-flocculent S. cerevisiae BY4741 strain at 33.92 ± 0.99 g L-1 after 24 h fermentation. This result was correlated with higher gene expressions of the sucrose-hydrolysing enzyme invertase, sugar phosphorylation, and pyruvate-to-ethanol pathways in the F118 strain compared with the BY4741 strain. Sequential fed-batch fermentation was conducted, and the F118 strain was easily separated from the fermentation broth via the formation of flocs and sediment. After the 5th cycle of fermentation with the F118 strain, the ethanol concentration reached 100.37 g L-1.

  Oct. 2018, Bioresource technology, 265, 542 - 547, English, International magazine

  [Refereed]

  Scientific journal


• Development of a strictly regulated xylose-induced expression system in Streptomyces.

  Yuji Noguchi, Norimasa Kashiwagi, Atsuko Uzura, Chiaki Ogino, Akihiko Kondo, Haruo Ikeda, Masahiro Sota

  BACKGROUND: Genetic tools including constitutive and inducible promoters have been developed over the last few decades for strain engineering in Streptomyces. Inducible promoters are useful for controlling gene expression, however only a limited number are applicable to Streptomyces. The aim of this study is to develop a controllable protein expression system based on an inducible promoter using sugar inducer, which has not yet been widely applied in Streptomyces. RESULTS: To determine a candidate promoter, inducible protein expression was first examined in Streptomyces avermitilis MA-4680 using various carbon sources. Xylose isomerase (xylA) promoter derived from xylose (xyl) operon was selected due to strong expression of xylose isomerase (XylA) in the presence of D-xylose. Next, a xylose-inducible protein expression system was constructed by investigating heterologous protein expression (chitobiase as a model protein) driven by the xylA promoter in Streptomyces lividans. Chitobiase activity was detected at high levels in S. lividans strain harboring an expression vector with xylA promoter (pXC), under both xylose-induced and non-induced conditions. Thus, S. avermitilis xylR gene, which encodes a putative repressor of xyl operon, was introduced into constructed vectors in order to control protein expression by D-xylose. Among strains constructed in the study, XCPR strain harboring pXCPR vector exhibited strict regulation of protein expression. Chitobiase activity in the XCPR strain was observed to be 24 times higher under xylose-induced conditions than that under non-induced conditions. CONCLUSION: In this study, a strictly regulated protein expression system was developed based on a xylose-induced system. As far as we could ascertain, this is the first report of engineered inducible protein expression in Streptomyces by means of a xylose-induced system. This system might be applicable for controllable expression of toxic products or in the field of synthetic biology using Streptomyces strains.

  Sep. 2018, Microbial cell factories, 17(1) (1), 151 - 151, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Selection of oleaginous yeasts capable of high lipid accumulation during challenges from inhibitory chemical compounds

  Ario Betha Juanssilfero, Prihardi Kahar, Rezky Lastinov Amza, Nao Miyamoto, Hiromi Otsuka, Hana Matsumoto, Chie Kihira, Ahmad Thontowi, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  Elsevier B.V., Sep. 2018, Biochemical Engineering Journal, 137, 182 - 191, English

  [Refereed]

  Scientific journal


• 油脂酵母Lipomyces starkeyiD35株の繰り返し発酵による脂肪酸組成変化解析

  宮本 捺央, Kahar Prihardi, 荻野 千秋, 近藤 昭彦

  (公社)日本生物工学会, Aug. 2018, 日本生物工学会大会講演要旨集, 平成30年度, 113 - 113, Japanese


• Engineering Human Epidermal Growth Receptor 2-Targeting Hepatitis B Virus Core Nanoparticles for siRNA Delivery in Vitro and in Vivo.

  Izzat F M Suffian, Julie T-W Wang, Farid N Faruqu, Julio Benitez, Yuya Nishimura, Chiaki Ogino, Akihiko Kondo, Khuloud T Al-Jamal

  Hepatitis B virus core (HBc) particles acquire the capacity to disassemble and reassemble in a controlled manner, allowing entrapment and delivery of drugs and macromolecules to cells. HBc particles are made of 180-240 copies of 21 kDa protein monomers, assembled into 30-34 nm diameter icosahedral particles. In this study, we aimed at formulating HBc particles for the delivery of siRNA for gene silencing in vitro and in vivo. We have previously reported recombinant HBc particles expressing ZHER2 affibodies, specifically targeting human epidermal growth receptor 2 (HER2)-expressing cancer cells (ZHER2-ΔHBc). siRNA was encapsulated within the ZHER2-ΔHBc particles following disassembly and reassembly. The ZHER2-ΔHBc-siRNA hybrids were able to secure the encapsulated siRNA from serum and nucleases in vitro. Enhanced siRNA uptake in HER2-expressing cancer cells treated with ZHER2-ΔHBc-siRNA hybrids was observed compared to the nontargeted HBc-siRNA hybrids in a time- and dose-dependent manner. A successful in vitro polo-like kinase 1 (PLK1) gene knockdown was demonstrated in cancer cells treated with ZHER2-ΔHBc-siPLK1 hybrids, to levels comparable to commercial transfecting reagents. Interestingly, ZHER2-ΔHBc particles exhibit intrinsic capability of reducing the solid tumor mass, independent of siPLK1 therapy, in an intraperitoneal tumor model following intraperitoneal injection.

  Jul. 2018, ACS applied nano materials, 1(7) (7), 3269 - 3282, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Metabolic engineering of Corynebacterium glutamicum for production of sunscreen shinorine.

  Yota Tsuge, Hideo Kawaguchi, Shogo Yamamoto, Yoshiko Nishigami, Masahiro Sota, Chiaki Ogino, Akihiko Kondo

  Ultraviolet-absorbing chemicals are useful in cosmetics and skin care to prevent UV-induced skin damage. We demonstrate here that heterologous production of shinorine, which shows broad absorption maxima in the UV-A and UV-B region. A shinorine producing Corynebacterium glutamicum strain was constructed by expressing four genes from Actinosynnema mirum DSM 43827, which are responsible for the biosynthesis of shinorine from sedoheptulose-7-phosphate in the pentose phosphate pathway. Deletion of transaldolase encoding gene improved shinorine production by 5.2-fold. Among the other genes in pentose phosphate pathway, overexpression of 6-phosphogluconate dehydrogenase encoding gene further increased shinorine production by 60% (19.1 mg/L). The genetic engineering of the pentose phosphate pathway in C. glutamicum improved shinorine production by 8.3-fold in total, and could be applied to produce the other chemicals derived from sedoheptulose-7-phosphate.

  Jul. 2018, Bioscience, biotechnology, and biochemistry, 82(7) (7), 1252 - 1259, English, International magazine

  [Refereed]

  Scientific journal


• In vivo tissue distribution and safety of polyacrylic acid-modified titanium peroxide nanoparticles as novel radiosensitizers.

  Kenta Morita, Takahiro Suzuki, Yuya Nishimura, Kazuhisa Matsumoto, Chiya Numako, Kazuyoshi Sato, Masao Nakayama, Ryohei Sasaki, Chiaki Ogino, Akihiko Kondo

  Polyacrylic acid (PAA)-modified titanium peroxide nanoparticles (PAA-TiOx NPs) are promising radiosensitizers. PAA-TiOx NPs were synthesized from commercial TiO2 nanoparticles that were modified with PAA and functionalized by H2O2 treatment. To realize practical clinical uses for PAA-TiOx NPs, their tissue distribution and acute toxicity were evaluated using healthy mice and mice bearing tumors derived from xenografted MIAPaCa-2 human pancreatic cancer cells. Healthy mice were injected with PAA-TiOx NPs at 25 mg/kg body weight via the tail vein, and tumor-bearing mice were injected either into the tumor locally or via the tail vein. The concentration of PAA-TiOx NPs in major organs was determined over time using inductively coupled-plasma atomic emission spectrometry. After 1 h, 12% of the PAA-TiOx NP dose had accumulated in the tumor, and 2.8% of the dose remained after 1 week. Such high accumulation could be associated with enhanced permeability and retention effects of the tumor, as PAA-TiOx NPs are composed of inorganic particles and polymers, without tumor-targeting molecules. The liver accumulated the largest proportion of the injected nanoparticles, up to 42% in tumor-bearing mice. Blood biochemical parameters were also investigated after intravenous injection of PAA-TiOx NPs in healthy mice. PAA-TiOx NPs invoked a slight change in various liver-related biochemical parameters, but no liver injury was observed over the practical dose range. In the future, PAA-TiOx NPs should be modified to prevent accumulation in the liver and minimize risk to patients.

  Jul. 2018, Journal of bioscience and bioengineering, 126(1) (1), 119 - 125, English, Domestic magazine

  [Refereed]

  Scientific journal


• Effect of inoculum size on single-cell oil production from glucose and xylose using oleaginous yeast Lipomyces starkeyi

  Ario B. Juanssilfero, Prihardi Kahar, Rezky L. Amza, Nao Miyamoto, Hiromi Otsuka, Hana Matsumoto, Chie Kihira, Ahmad Thontowi, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  Elsevier B.V., Jun. 2018, Journal of Bioscience and Bioengineering, 125(6) (6), 695 - 702, English

  [Refereed]

  Scientific journal


• Metabolome analysis-based design and engineering of a metabolic pathway in Corynebacterium glutamicum to match rates of simultaneous utilization of D-glucose and L-arabinose.

  Hideo Kawaguchi, Kumiko Yoshihara, Kiyotaka Y Hara, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  BioMed Central Ltd., May 2018, Microbial cell factories, 17(1) (1), 76 - 76, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 新規放射線増感剤としての過酸化チタンナノ粒子の物性解析

  森田 健太, 西村 勇哉, 大取 靖秀, 荒井 優紀, 沼子 千弥, 荻野 千秋

  日本DDS学会, May 2018, 日本DDS学会学術集会プログラム予稿集, 34回, 207 - 207, Japanese


• Effective usage of sorghum bagasse: Optimization of organosolv pretreatment using 25% 1-butanol and subsequent nanofiltration membrane separation

  Hiroshi Teramura, Kengo Sasaki, Tomoko Oshima, Hideo Kawaguchi, Chiaki Ogino, Takashi Sazuka, Akihiko Kondo

  Mar. 2018, Bioresource Technology, 252, 157 - 164, English

  [Refereed]

  Scientific journal


• Pretreatment of bagasse with a minimum amount of cholinium ionic liquid for subsequent saccharification at high loading and co-fermentation for ethanol production

  Kazuaki Ninomiya, Amaliyah Rohsari Indah Utami, Yota Tsuge, Kosuke Kuroda, Chiaki Ogino, Tetsuya Taima, Joji Saito, Mitsugu Kimizu, Kenji Takahashi

  Elsevier B.V., Feb. 2018, Chemical Engineering Journal, 334, 657 - 663, English

  [Refereed]

  Scientific journal


• Lignocellulose nanofibers prepared by ionic liquid pretreatment and subsequent mechanical nanofibrillation of bagasse powder: Application to esterified bagasse/polypropylene composites

  Kazuaki Ninomiya, Megumi Abe, Takayuki Tsukegi, Kosuke Kuroda, Yota Tsuge, Chiaki Ogino, Kentaro Taki, Tetsuya Taima, Joji Saito, Mitsugu Kimizu, Kiyoshi Uzawa, Kenji Takahashi

  Feb. 2018, CARBOHYDRATE POLYMERS, 182, 8 - 14, English

  [Refereed]

  Scientific journal


• P-45 Caffeic acid production by simultaneous saccharification and fermentation of kraft pulp using recombinant Escherichia coli

  Kawaguchi Hideo, Katsuyama Yohei, Du Danyao, Nakamura-Tsuruta Sachiko, Minami Hiromichi, Ogino Chiaki, Ohnishi Yasuo, Kondo Akihiko

  Caffeic acid (3,4-dihydroxycinnamic acid) serves as a building block for thermoplastics and was recently produced from glucose by microbial fermentation. To produce caffeic acid from inedible cellulose, separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) reactions were compared using kraft pulp as lignocellulosic feedstock. Here, a tyrosine-overproducing Escherichia coli strain was metabolically engineered to produce caffeic acid from glucose by expressing the genes encoding a tyrosine ammonia lyase (fevV) from Streptomyces sp. WK-5344 and 4-hydroxyphenyllactate 3-hydroxylase (hpaBC) from Pseudomonas aeruginosa. Using the resulting recombinant strain, the maximum yield of caffeic acid in SSF (233 mg/L) far exceeded that by SHF (37.9 mg/L). In the SSF with low cellulase loads (≤2.5 filter paper unit/g glucan), caffeic acid production was markedly increased, while almost no glucose accumulation was detected, indicating that the E. coli cells experienced glucose limitation in this culture condition. Caffeic acid yield was also negatively correlated with the glucose concentration in the fermentation medium. These observations suggest that glucose limitation in SSF is crucial for improving caffeic acid yield, owing to reduced by-product formation and fermentation inhibitor accumulation.

  The Japan Institute of Energy, 2018, Proceedings of the Conference on Biomass Science, 13(0) (0), 135 - 136, Japanese

  [Refereed]


• Oxidative depolymerization potential of biorefinery lignin obtained by ionic liquid pretreatment and subsequent enzymatic saccharification of eucalyptus

  Kazuaki Ninomiya, Kobako Ochiai, Mao Eguchi, Kosuke Kuroda, Yota Tsuge, Chiaki Ogino, Tetsuya Taima, Kenji Takahashi

  Elsevier B.V., Jan. 2018, Industrial Crops and Products, 111, 457 - 461, English

  Scientific journal


• Xylanase and feruloyl esterase from actinomycetes cultures could enhance sugarcane bagasse hydrolysis in the production of fermentable sugars

  Nanik Rahmani, Prihardi Kahar, Puspita Lisdiyanti, Euis Hermiati, Jaemin Lee, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

  Japan Society for Bioscience Biotechnology and Agrochemistry, 2018, Bioscience, Biotechnology and Biochemistry, 82(5) (5), 904 - 915, English

  [Refereed]

  Scientific journal


• Mixing characteristics of submerged fungal fluid in a flexible stirred mixer system

  Narges Ghobadi, Chiaki Ogino, Tomohiro Ogawa, Naoto Ohmura

  Society of Chemical Engineers, Japan, 2018, Journal of Chemical Engineering of Japan, 51(2) (2), 143 - 151, English

  [Refereed]

  Scientific journal


• Genotypic effects on sugar and by-products of liquid hydrolysates and on saccharification of acidinsoluble residues from wheat straw

  Ryoko Ohno, Hiroshi Teramura, Chiaki Ogino, Akihiko Kondo, Shigeo Takumi

  日本遺伝学会, 2018, Genes and Genetic Systems, 93(1) (1), 1 - 7, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Direct and highly productive conversion of cyanobacteria Arthrospira platensis to ethanol with CaCl2 addition.

  Shimpei Aikawa, Kentaro Inokuma, Satoshi Wakai, Kengo Sasaki, Chiaki Ogino, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo

  BioMed Central Ltd., 2018, Biotechnology for biofuels, 11(1) (1), 50 - 50, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Mathematical Model for Small Size Time Series Data of Bacterial Secondary Metabolic Pathways.

  Daisuke Tominaga, Hideo Kawaguchi, Yoshimi Hori, Tomohisa Hasunuma, Chiaki Ogino, Sachiyo Aburatani

  Measuring the concentrations of metabolites and estimating the reaction rates of each reaction step consisting of metabolic pathways are significant for an improvement in microorganisms used in maximizing the production of materials. Although the reaction pathway must be identified for such an improvement, doing so is not easy. Numerous reaction steps have been reported; however, the actual reaction steps activated vary or change according to the conditions. Furthermore, to build mathematical models for a dynamical analysis, the reaction mechanisms and parameter values must be known; however, to date, sufficient information has yet to be published for many cases. In addition, experimental observations are expensive. A new mathematical approach that is applicable to small sample data, and that requires no detailed reaction information, is strongly needed. S-system is one such model that can use smaller samples than other ordinary differential equation models. We propose a simplified S-system to apply minimal quantities of samples for a dynamic analysis of the metabolic pathways. We applied the model to the phenyl lactate production pathway of Escherichia coli. The model obtained suggests that actually activated reaction steps and feedback are inhibitions within the pathway.

  2018, Bioinformatics and biology insights, 12, 1177932218775076 - 1177932218775076, English, International magazine

  [Refereed]

  Scientific journal


• Genotypic effects on sugar and by-products of liquid hydrolysates and on saccharification of acidinsoluble residues from wheat straw

  Ryoko Ohno, Hiroshi Teramura, Chiaki Ogino, Akihiko Kondo, Shigeo Takumi

  2018, Genes and Genetic Systems, 93(1) (1), 1 - 7, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Biotransformation of ferulic acid to protocatechuic acid by Corynebacterium glutamicum ATCC 21420 engineered to express vanillate O-demethylase.

  Naoko Okai, Takaya Masuda, Yasunobu Takeshima, Kosei Tanaka, Ken-Ichi Yoshida, Masanori Miyamoto, Chiaki Ogino, Akihiko Kondo

  Ferulic acid (4-hydroxy-3-methoxycinnamic acid, FA) is a lignin-derived phenolic compound abundant in plant biomass. The utilization of FA and its conversion to valuable compounds is desired. Protocatechuic acid (3,4-dihydroxybenzoic acid, PCA) is a precursor of polymers and plastics and a constituent of food. A microbial conversion system to produce PCA from FA was developed in this study using a PCA-producing strain of Corynebacterium glutamicum F (ATCC 21420). C. glutamicum strain F grown at 30 °C for 48 h utilized 2 mM each of FA and vanillic acid (4-hydroxy-3-methoxybenzoic acid, VA) to produce PCA, which was secreted into the medium. FA may be catabolized by C. glutamicum through proposed (I) non-β-oxidative, CoA-dependent or (II) β-oxidative, CoA-dependent phenylpropanoid pathways. The conversion of VA to PCA is the last step in each pathway. Therefore, the vanillate O-demethylase gene (vanAB) from Corynebacterium efficiens NBRC 100395 was expressed in C. glutamicum F (designated strain FVan) cultured at 30 °C in AF medium containing FA. Strain C. glutamicum FVan converted 4.57 ± 0.07 mM of FA into 2.87 ± 0.01 mM PCA after 48 h with yields of 62.8% (mol/mol), and 6.91 mM (1064 mg/L) of PCA was produced from 16.0 mM of FA after 12 h of fed-batch biotransformation. Genomic analysis of C. glutamicum ATCC 21420 revealed that the PCA-utilization genes (pca cluster) were conserved in strain ATCC 21420 and that mutations were present in the PCA importer gene pcaK.

  Dec. 2017, AMB Express, 7(1) (1), 130 - 130, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Production of chemicals and proteins using biomass-derived substrates from a Streptomyces host

  Norimasa Kashiwagi, Chiaki Ogino, Akihiko Kondo

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245, 1655 - 1663, English

  [Refereed]

  Scientific journal


• Microbial conversion of biomass into bio-based polymers

  Hideo Kawaguchi, Chiaki Ogino, Akihiko Kondo

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245, 1664 - 1673, English

  [Refereed]

  Scientific journal


• Future insights in fungal metabolic engineering

  Satoshi Wakai, Takayoshi Arazoe, Chiaki Ogino, Akihiko Kondo

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245, 1314 - 1326, English

  [Refereed]

  Scientific journal


• Development and evaluation of consolidated bioprocessing yeast for ethanol production from ionic liquid-pretreated bagasse

  Jerome Amoah, Naoya Ishizue, Miki Ishizaki, Misa Yasuda, Kenji Takahashi, Kazuaki Ninomiya, Ryosuke Yamada, Akihiko Kondo, Chiaki Ogino

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245, 1413 - 1420, English

  [Refereed]

  Scientific journal


• Challenges of non-flocculating Saccharomyces cerevisiae haploid strain against inhibitory chemical complex for ethanol production

  Prihardi Kahar, Eny Ida Riyanti, Hiromi Otsuka, Hana Matsumoto, Chie Kihira, Chiaki Ogino, Akihiko Kondo

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245, 1436 - 1446, English

  [Refereed]

  Scientific journal


• Ionic liquid pretreatment of bagasse improves mechanical property of bagasse/polypropylene composites

  Kazuaki Ninomiya, Megumi Abe, Takayuki Tsukegi, Kosuke Kuroda, Masaaki Omichi, Kenji Takada, Mana Noguchi, Yota Tsuge, Chiaki Ogino, Kentaro Taki, Tetsuya Taima, Kiyoshi Uzawa, Kenji Takahashi

  Dec. 2017, INDUSTRIAL CROPS AND PRODUCTS, 109, 158 - 162, English

  [Refereed]

  Scientific journal


• Glutathione production from mannan-based bioresource by mannanase/mannosidase expressing Saccharomyces cerevisiae

  Alex Prima, Kiyotaka Y. Hara, Apridah Cameliawati Djohan, Norimasa Kashiwagi, Prihardi Kahar, Jun Ishii, Hideki Nakayama, Fumiyoshi Okazaki, Bambang Prasetya, Akihiko Kondo, Yopi, Chiaki Ogino

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245, 1400 - 1406, English

  [Refereed]

  Scientific journal


• Conversion of Chlamydomonas sp JSC4 lipids to biodiesel using Fusarium heterosporum lipase-expressing Aspergillus oryzae whole-cell as biocatalyst

  Jerome Amoah, Shih-Hsin Ho, Shinji Hama, Ayumi Yoshida, Akihito Nakanishi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Dec. 2017, ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 28, 16 - 23, English

  [Refereed]

  Scientific journal


• Acceleration of wound healing by ultrasound activation of TiO2 in Escherichia coli-infected wounds in mice

  Koji Osumi, Sachiko Matsuda, Naoki Fujimura, Kentaro Matsubara, Minoru Kitago, Osamu Itano, Chiaki Ogino, Nobuaki Shimizu, Hideaki Obara, Yuko Kitagawa

  John Wiley and Sons Inc., Nov. 2017, Journal of Biomedical Materials Research - Part B Applied Biomaterials, 105(8) (8), 2344 - 2351, English

  [Refereed]

  Scientific journal


• Screening and evaluation of aptamers against somatostatin, and sandwich-like monitoring of somatostatin based on atomic force microscopy

  Musashi Takenaka, Tomokazu Amino, Yusuke Miyachi, Chiaki Ogino, Akihiko Kondo

  Nov. 2017, SENSORS AND ACTUATORS B-CHEMICAL, 252, 813 - 821, English

  [Refereed]

  Scientific journal


• Bear-trap sensing of somatostatin via split aptamers and atomic force microscopy

  Musashi Takenaka, Tomokazu Amino, Yusuke Miyachi, Chiaki Ogino, Akihiko Kondo

  Nov. 2017, SENSORS AND ACTUATORS B-CHEMICAL, 252, 600 - 605, English

  [Refereed]

  Scientific journal


• Sucrose purification and repeated ethanol production from sugars remaining in sweet sorghum juice subjected to a membrane separation process.

  Kengo Sasaki, Yota Tsuge, Hideo Kawaguchi, Masahiro Yasukawa, Daisuke Sasaki, Takashi Sazuka, Eiji Kamio, Chiaki Ogino, Hideto Matsuyama, Akihiko Kondo

  The juice from sweet sorghum cultivar SIL-05 (harvested at physiological maturity) was extracted, and the component sucrose and reducing sugars (such as glucose and fructose) were subjected to a membrane separation process to purify the sucrose for subsequent sugar refining and to obtain a feedstock for repeated bioethanol production. Nanofiltration (NF) of an ultrafiltration (UF) permeate using an NTR-7450 membrane (Nitto Denko Corporation, Osaka, Japan) concentrated the juice and produced a sucrose-rich fraction (143.2 g L-1 sucrose, 8.5 g L-1 glucose, and 4.5 g L-1 fructose). In addition, the above NF permeate was concentrated using an ESNA3 NF membrane to provide concentrated permeated sugars (227.9 g L-1) and capture various amino acids in the juice, enabling subsequent ethanol fermentation without the addition of an exogenous nitrogen source. Sequential batch fermentation using the ESNA3 membrane concentrate provided an ethanol titer and theoretical ethanol yield of 102.5-109.5 g L-1 and 84.4-89.6%, respectively, throughout the five-cycle batch fermentation by Saccharomyces cerevisiae BY4741. Our results demonstrate that a membrane process using UF and two types of NF membranes has the potential to allow sucrose purification and repeated bioethanol production.

  Aug. 2017, Applied microbiology and biotechnology, 101(15) (15), 6007 - 6014, English, International magazine

  [Refereed]

  Scientific journal


• Caffeic acid production by simultaneous saccharification and fermentation of kraft pulp using recombinant Escherichia coli

  Hideo Kawaguchi, Yohei Katsuyama, Du Danyao, Prihardi Kahar, Sachiko Nakamura-Tsuruta, Hiroshi Teramura, Keiko Wakai, Kumiko Yoshihara, Hiromichi Minami, Chiaki Ogino, Yasuo Ohnishi, Ahikiko Kondo

  Jul. 2017, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 101(13) (13), 5279 - 5290, English

  [Refereed]

  Scientific journal


• Direct Ethanol Production from Ionic Liquid-Pretreated Lignocellulosic Biomass by Cellulase-Displaying Yeasts

  Ryosuke Yamada, Kazunori Nakashima, Nanami Asai-Nakashima, Wataru Tokuhara, Nobuhiro Ishida, Satoshi Katahira, Noriho Kamiya, Chiaki Ogino, Akihiko Kondo

  May 2017, APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 182(1) (1), 229 - 237, English

  [Refereed]

  Scientific journal


• Mannan endo-1,4-beta-mannosidase from Kitasatospora sp isolated in Indonesia and its potential for production of mannooligosaccharides from mannan polymers

  Nanik Rahmani, Norimasa Kashiwagi, JaeMin Lee, Satoko Niimi-Nakamura, Hana Matsumoto, Prihardi Kahar, Puspita Lisdiyanti, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

  May 2017, AMB EXPRESS, 7, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Yield Optimisation of Hepatitis B Virus Core Particles in E-coli Expression System for Drug Delivery Applications

  Izzat Fahimuddin Bin Mohamed Suffian, Mitla Garcia-Maya, Paul Brown, Tam Bui, Yuya Nishimura, Amir Rafiq Bin Mohammad Johari Palermo, Chiaki Ogino, Akihiko Kondo, Khuloud T. Al-Jamal

  Mar. 2017, SCIENTIFIC REPORTS, 7, 43160, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Simultaneous conversion of free fatty acids and triglycerides to biodiesel by immobilized Aspergillus oryzae expressing Fusarium heterosporum lipase

  Jerome Amoah, Emmanuel Quayson, Shinji Hama, Ayumi Yoshida, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Mar. 2017, BIOTECHNOLOGY JOURNAL, 12(3) (3), 1600400, English

  [Refereed]

  Scientific journal


• Mapping of endoglucanases displayed on yeast cell surface using atomic force microscopy

  Musashi Takenaka, Takuya Kobayashi, Kentaro Inokuma, Tomohisa Hasunuma, Tatsuo Maruyama, Chiaki Ogino, Akihiko Kondo

  Mar. 2017, COLLOIDS AND SURFACES B-BIOINTERFACES, 151, 134 - 142, English

  [Refereed]

  Scientific journal


• Engineering hepatitis B virus core particles for targeting HER2 receptors in vitro and in vivo

  Izzat Fahimuddin Bin Mohamed Suffian, Julie Tzu-Wen Wang, Naomi O. Hodgins, Rebecca Klippstein, Mitla Garcia-Maya, Paul Brown, Yuya Nishimura, Hamed Heidari, Sara Bals, Jane K. Sosabowski, Chiaki Ogino, Akihiko Kondo, Khuloud T. Al-Jamal

  Mar. 2017, BIOMATERIALS, 120, 126 - 138, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• DNA-duplex linker for AFM-SELEX of DNA aptamer against human serum albumin

  Musashi Takenaka, Yuzo Okumura, Tomokazu Amino, Yusuke Miyachi, Chiaki Ogino, Akihiko Kondo

  Feb. 2017, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 27(4) (4), 954 - 957, English

  [Refereed]

  Scientific journal


• Characterizations of the submerged fermentation of Aspergillus oryzae using a Fullzone impeller in a stirred tank bioreactor

  Narges Ghobadi, Chiaki Ogino, Kaoru Yamabe, Naoto Ohmura

  Jan. 2017, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 123(1) (1), 101 - 108, English

  [Refereed]

  Scientific journal


• Study the Relation between Fermentation Characteristics of Submerged Fluid and Improving the Lactic Acid Production by Fungi

  Narges Ghobadi, Naoya Sasakura, Satoshi Wakai, Chiaki Ogino, Kaoru Yamabe, OHMURA NAOTO

  2017, Journal of Bioprocessing & Biotechniques, 7(2) (2), 300: 1 - 5, English

  [Refereed]

  Scientific journal


• Affibody-displaying bio-nanocapsules effective in EGFR, typical biomarker, expressed in various cancer cells

  Yuya Nishimura, Ryosuke Ezawa, Jun Ishii, Chiaki Ogino, Akihiko Kondo

  Jan. 2017, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 27(2) (2), 336 - 341, English

  [Refereed]

  Scientific journal


• Differences in glucose yield of residues from among varieties of rice, wheat, and sorghum after dilute acid pretreatment

  Hiroshi Teramura, Kengo Sasaki, Hideo Kawaguchi, Fumio Matsuda, Jun Kikuchi, Tomokazu Shirai, Takashi Sazuka, Masanori Yamasaki, Shigeo Takumi, Chiaki Ogino, Akihiko Kondo

  2017, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 81(8) (8), 1650 - 1656, English

  [Refereed]

  Scientific journal


• Overexpression of CO2-responsive CCT protein, a key regulator of starch synthesis strikingly increases the glucose yield from rice straw for bioethanol production

  Ryutaro Morita, Hiroshi Teramura, Chiaki Ogino, Akihiko Kondo, Hiroshi Fukayama

  2017, PLANT PRODUCTION SCIENCE, 20(4) (4), 441 - 447, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Comprehension of an organosolv process for lignin extraction on Festuca arundinacea and monitoring of the cellulose degradation

  Quentin Schmetz, Guillaume Maniet, Nicolas Jacquet, Hiroshi Teramura, Chiaki Ogino, Akihiko Kondo, Aurore Richel

  Dec. 2016, INDUSTRIAL CROPS AND PRODUCTS, 94, 308 - 317, English

  [Refereed]

  Scientific journal


• Characterization of titanium dioxide nanoparticles modified with polyacrylic acid and H2O2 for use as a novel radiosensitizer

  Kenta Morita, Serika Miyazaki, Chiya Numako, Shinya Ikeno, Ryohei Sasaki, Yuya Nishimura, Chiaki Ogino, Akihiko Kondo

  Dec. 2016, FREE RADICAL RESEARCH, 50(12) (12), 1319 - 1328, English

  [Refereed]

  Scientific journal


• Bioprocessing of bio-based chemicals produced from lignocellulosic feedstocks

  Hideo Kawaguchi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Dec. 2016, CURRENT OPINION IN BIOTECHNOLOGY, 42, 30 - 39, English

  [Refereed]

  Scientific journal


• Using a flexible shaft agitator to enhance the rheology of a complex fungal fermentation culture

  Narges Ghobadi, Chiaki Ogino, Tomohiro Ogawa, Naoto Ohmura

  Nov. 2016, BIOPROCESS AND BIOSYSTEMS ENGINEERING, 39(11) (11), 1793 - 1801, English

  [Refereed]

  Scientific journal


• Intensifying the fermentation of Aspergillus oryzae in a stirred bioreactor using maxblend impeller

  Narges Ghobadi, Chiaki Ogino, Naoto Ohmura

  Bentham Science Publishers B.V., Oct. 2016, Open Chemical Engineering Journal, 10, 88 - 109, English

  [Refereed]

  Scientific journal


• Effect of Swingstir impeller on rheology and morphology of submerged fermentation

  Narges Ghobadi, Chiaki Ogino, Tomohiro Ogawa, Naoto Ohmura

  Sep. 2016, Proceedings of 5th Asian Conference on Mixing, 2016 (ACOM2016), 45 - 50, English

  [Refereed]

  International conference proceedings


• From mannan to bioethanol: cell surface co-display of beta-mannanase and beta-mannosidase on yeast Saccharomyces cerevisiae

  Jun Ishii, Fumiyoshi Okazaki, Apridah Cameliawati Djohan, Kiyotaka Y. Hara, Nanami Asai-Nakashima, Hiroshi Teramura, Ade Andriani, Masahiro Tominaga, Satoshi Wakai, Prihardi Kahar, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

  Sep. 2016, BIOTECHNOLOGY FOR BIOFUELS, 9(1) (1), 188, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Enhancement of astaxanthin production in Xanthophyllomyces dendrorhous by efficient method for the complete deletion of genes

  Keisuke Yamamoto, Kiyotaka Y. Hara, Toshihiko Morita, Akira Nishimura, Daisuke Sasaki, Jun Ishii, Chiaki Ogino, Noriyuki Kizaki, Akihiko Kondo

  Sep. 2016, MICROBIAL CELL FACTORIES, 15(1) (1), 155, English

  [Refereed]

  Scientific journal


• Lipase cocktail for efficient conversion of oils containing phospholipids to biodiesel

  Jerome Amoah, Shih-Hsin Ho, Shinji Hama, Ayumi Yoshida, Akihito Nakanishi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Jul. 2016, BIORESOURCE TECHNOLOGY, 211, 224 - 230, English

  [Refereed]

  Scientific journal


• Titanium peroxide nanoparticles enhanced cytotoxic effects of X-ray irradiation against pancreatic cancer model through reactive oxygen species generation in vitro and in vivo

  Masao Nakayama, Ryohei Sasaki, Chiaki Ogino, Tsutomu Tanaka, Kenta Morita, Mitsuo Umetsu, Satoshi Ohara, Zhenquan Tan, Yuya Nishimura, Hiroaki Akasaka, Kazuyoshi Sato, Chiya Numako, Seiichi Takami, Akihiko Kondo

  Jul. 2016, RADIATION ONCOLOGY, 11(1) (1), 91, English

  [Refereed]

  Scientific journal


• Sonocatalytic injury of cancer cells attached on the surface of a nickel-titanium dioxide alloy plate

  Kazuaki Ninomiya, Hirotaka Maruyama, Chiaki Ogino, Kenji Takahashi, Nobuaki Shimizu

  Elsevier, Jun. 2016, Ultrasonics Sonochemistry, 28, 1 - 6, English

  Scientific journal


• Natural variation in the glucose content of dilute sulfuric acid-pretreated rice straw liquid hydrolysates: implications for bioethanol production

  Takashi Goda, Hiroshi Teramura, Miki Suehiro, Kengo Kanamaru, Hideo Kawaguchi, Chiaki Ogino, Akihiko Kondo, Masanori Yamasaki

  May 2016, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 80(5) (5), 863 - 869, English

  [Refereed]

  Scientific journal


• Engineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel production

  Zhuo Liu, Shih-Hsin Ho, Kengo Sasaki, Riaan den Haan, Kentaro Inokuma, Chiaki Ogino, Willem H. van Zyl, Tomohisa Hasunuma, Akihiko Kondo

  Apr. 2016, SCIENTIFIC REPORTS, 6, 24550, English

  [Refereed]

  Scientific journal


• Organosolv pretreatment of sorghum bagasse using a low concentration of hydrophobic solvents such as 1-butanol or 1-pentanol

  Hiroshi Teramura, Kengo Sasaki, Tomoko Oshima, Fumio Matsuda, Mami Okamoto, Tomokazu Shirai, Hideo Kawaguchi, Chiaki Ogino, Ko Hirano, Takashi Sazuka, Hidemi Kitano, Jun Kikuchi, Akihiko Kondo

  Feb. 2016, BIOTECHNOLOGY FOR BIOFUELS, 9, 27, English

  [Refereed]

  Scientific journal


• Characterization of cellulose nanofiber sheets from different refining processes

  Kohji Nobuta, Hiroshi Teramura, Hiroaki Ito, Chizuru Hongo, Hideo Kawaguchi, Chiaki Ogino, Akihiko Kondo, Takashi Nishino

  Feb. 2016, CELLULOSE, 23(1) (1), 403 - 414, English

  [Refereed]

  Scientific journal


• Study of Titanium Peroxide Nanoparticles for Novel Radiation Therapy

  OGINO Chiaki

  Radiosensizing therapy, combining radiotherapy and an agent for amplifying radiation, have an advantage for therapy of low-oxygen cancer including pancreatic. In our recent study, the hydrogen peroxide treatment of titanium dioxide nanoparticles induced titanium peroxide nanoparticle (PAATiOx), and it was suggested to use as radiosensitizing agent for radiotherapy. By this combination, the apparent therapeutic positive effect was observed in vivo study of human pancreatic xenograft mice. However, it can't be said that it has a high usefulness for radiosensizing therapy. So we prepared antibody modification PAATiOx (PAATiOx/1a), the antibody is the variable domain of a camelid heavy chain-only antibody (1a), which has high affinity to EGFR, expressed in various cancer cells. As a result, PAATiOx/1a with X-ray treatment showed more cytotoxicity than PAATiOx with equivalent dose of X-ray irradiation in vitro and in vivo. Since this study showed that PAATiOx/1a could target EGFR-expressing cells, we would use this particle as a drug delivery carrier for various cancer cells expressing EGFR.

  Hosokawa Powder Technology Foundation, 2016, Hosokawa Powder Technology Foundation ANNUAL REPORT, 24, 30 - 34, Japanese


• Production of protocatechuic acid by Corynebacterium glutamicum expressing chorismate-pyruvate lyase from Escherichia coli.

  Naoko Okai, Takanori Miyoshi, Yasunobu Takeshima, Hiroaki Kuwahara, Chiaki Ogino, Akihiko Kondo

  Protocatechuic acid (3,4-dihydroxybenzoic acid; PCA) serves as a building block for polymers and pharmaceuticals. In this study, the biosynthetic pathway for PCA from glucose was engineered in Corynebacterium glutamicum. The pathway to PCA-employed elements of the chorismate pathway by using chorismate-pyruvate lyase (CPL) and 4-hydroxybenzoate hydroxylase (4-HBA hydroxylase). As C. glutamicum has the potential to synthesize the aromatic amino acid intermediate chorismate and possesses 4-HBA hydroxylase, we focused on expressing Escherichia coli CPL in a phenylalanine-producing strain of C. glutamicum ATCC21420. To secrete PCA, the gene (ubiC) encoding CPL from E. coli was expressed in C. glutamicum ATCC 21420 (strain F(UbiC)). The formation of 28.8 mg/L of extracellular 4-HBA (36 h) and 213 ± 29 mg/L of extracellular PCA (80 h) was obtained by the C. glutamicum strain F(UbiC) from glucose. The strain ATCC21420 was also found to produce extracellular PCA. PCA fermentation was performed using C. glutamicum strain F(UbiC) in a bioreactor at the optimized pH of 7.5. C. glutamicum F(UbiC) produced 615 ± 2.1 mg/L of PCA from 50 g/L of glucose after 72 h. Further, fed-batch fermentation of PCA by C. glutamicum F(UbiC) was performed with feedings of glucose every 24 h. The maximum production of PCA (1140.0 ± 11.6 mg/L) was achieved when 117.0 g/L of glucose was added over 96 h of fed-batch fermentation.

  Jan. 2016, Applied microbiology and biotechnology, 100(1) (1), 135 - 45, English, International magazine

  [Refereed]


• O-18 Simultaneous saccharification and fermentation of lignocellulosic biomass for phenyllactate production by recombinant Escherichia coli

  川口,秀夫, 寺村,浩, 中村,聡子, 荻野,千秋, 原,清敬, 蓮沼,誠久, 老沼,研一, 高谷,直樹, 平野,恒, 佐塚,隆志, 北野,英己, 近藤,昭彦

  Sorghum bagasse pretreated with diluted acid, which was predominantly composed of glucan (59%) and xylan (7.2%), was used as a lignocellulosic feedstock to produce D-phenyllactic acid (PhLA) by a recombinant Escherichia coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens. Compared to filter paper hydrolysate, the PhLA yield was reduced by 35% during fermentation with enzymatic hydrolysate of sorghum bagasse as a carbon source, and metabolomics analysis revealed that intracellular levels of erythrose-4-phosphate and phosphoenolpyruvate and NAD(P)H regeneration for PhLA production from glucose markedly reduced. Compared to the separate hydrolysis and fermentation (SHF) with sorghum bagasse hydrolysate, simultaneous saccharification and fermentation (SSF) of sorghum bagasse under glucose limitation conditions yielded 4.8-fold more PhLA with less accumulation of eluted components of p-coumaric acid and aldehydes, which inhibited PhLA fermentation. These results suggest that gradual hydrolysis of sorghum bagasse during SSF reduces the accumulation of both glucose and fermentation inhibitors, collectively leading to increased PhLA yield.

  The Japan Institute of Energy, Jan. 2016, Proceedings of the Conference on Biomass Science, 11, 35 - 36, Japanese

  [Refereed]

  International conference proceedings


• Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae

  Kengo Sasaki, Kiyotaka Y. Hara, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

  Jan. 2016, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 121(1) (1), 96 - 100, English

  [Refereed]

  Scientific journal


• Converting oils high in phospholipids to biodiesel using immobilized Aspergillus oryzae whole-cell biocatalysts expressing Fusarium heterosporum lipase

  Jerome Amoah, Shih-Hsin Ho, Shinji Hama, Ayumi Yoshida, Akihito Nakanishi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Jan. 2016, BIOCHEMICAL ENGINEERING JOURNAL, 105, 10 - 15, English

  [Refereed]

  Scientific journal


• 3-Amino-4-hydroxybenzoic acid production from sweet sorghum juice by recombinant Corynebacterium glutamicum

  Hideo Kawaguchi, Kengo Sasaki, Kouji Uematsu, Yota Tsuge, Hiroshi Teramura, Naoko Okai, Sachiko Nakamura-Tsuruta, Yohei Katsuyama, Yoshinori Sugai, Yasuo Ohnishi, Ko Hirano, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

  Dec. 2015, BIORESOURCE TECHNOLOGY, 198, 410 - 417, English

  [Refereed]

  Scientific journal


• Effect of post-pretreatment washing on saccharification and co-fermentation from bagasse pretreated with biocompatible cholinium ionic liquid

  Kazuaki Ninomiya, Chiaki Ogino, Miki Ishizaki, Misa Yasuda, Nobuaki Shimizu, Kenji Takahashi

  Elsevier, Nov. 2015, Biochemical Engineering Journal, 103, 198 - 204, English

  Scientific journal


• Enzymatic synthesis and modification of structured phospholipids: recent advances in enzyme preparation and biocatalytic processes

  Shinji Hama, Chiaki Ogino, Akihiko Kondo

  Oct. 2015, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 99(19) (19), 7879 - 7891, English

  [Refereed]

  Scientific journal


• バイオリファイナリーの現状と展望

  HASUNUMA TOMOHISA, ISHII JUN, OGINO CHIAKI, KONDO AKIHIKO

  Oct. 2015, 化学と生物, 53(10) (10), 689 - 695, Japanese

  Scientific journal


• Saccharification and ethanol fermentation from cholinium ionic liquid-pretreated bagasse with a different number of post-pretreatment washings

  Kazuaki Ninomiya, Sayuri Omote, Chiaki Ogino, Kosuke Kuroda, Mana Noguchi, Takatsugu Endo, Ryohei Kakuchi, Nobuaki Shimizu, Kenji Takahashi

  Aug. 2015, Bioresource Technology, 189, 203 - 209, English

  [Refereed]

  Scientific journal


• Production of D-lactic acid from hardwood pulp by mechanical milling followed by simultaneous saccharification and fermentation using metabolically engineered Lactobacillus plantarum

  Shinji Hama, Shino Mizuno, Maki Kihara, Tsutomu Tanaka, Chiaki Ogino, Hideo Noda, Akihiko Kondo

  Jul. 2015, BIORESOURCE TECHNOLOGY, 187, 167 - 172, English

  [Refereed]

  Scientific journal


• Changes in Lignin and Polysaccharide Components in 13 Cultivars of Rice Straw following Dilute Acid Pretreatment as Studied by Solution-State 2D H-1-C-13 NMR

  Hiroshi Teramura, Kengo Sasaki, Tomoko Oshima, Shimpei Aikawa, Fumio Matsuda, Mami Okamoto, Tomokazu Shirai, Hideo Kawaguchi, Chiaki Ogino, Masanori Yamasaki, Jun Kikuchi, Akihiko Kondo

  Jun. 2015, PLOS ONE, 10(6) (6), e0128417, English

  [Refereed]

  Scientific journal


• Effective saccharification of kraft pulp by using a cellulase cocktail prepared from genetically engineered Aspergillus oryzae

  Ryosuke Yamada, Toshihide Yoshie, Shoji Sakai, Satoshi Wakai, Nanami Asai-Nakashima, Fumiyoshi Okazaki, Chiaki Ogino, Hiromoto Hisada, Hiroko Tsutsumi, Yoji Hata, Akihiko Kondo

  Jun. 2015, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 79(6) (6), 1034 - 1037, English

  [Refereed]

  Scientific journal


• Repeated ethanol production from sweet sorghum juice concentrated by membrane separation

  Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

  Jun. 2015, BIORESOURCE TECHNOLOGY, 186, 351 - 355, English

  [Refereed]

  Scientific journal


• Precipitate obtained following membrane separation of hydrothermally pretreated rice straw liquid revealed by 2D NMR to have high lignin content

  Kengo Sasaki, Mami Okamoto, Tomokazu Shirai, Yota Tsuge, Hiroshi Teramura, Daisuke Sasaki, Hideo Kawaguchi, Tomohisa Hasunuma, Chiaki Ogino, Fumio Matsuda, Jun Kikuchi, Akihiko Kondo

  Jun. 2015, BIOTECHNOLOGY FOR BIOFUELS, 8, 88, English

  [Refereed]

  Scientific journal


• Mechanical milling and membrane separation for increased ethanol production during simultaneous saccharification and co-fermentation of rice straw by xylose-fermenting Saccharomyces cerevisiae

  Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Hiroshi Teramura, Kentaro Inokuma, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Jun. 2015, BIORESOURCE TECHNOLOGY, 185, 263 - 268, English

  [Refereed]

  Scientific journal


• 新たな放射線増感剤としての過酸化チタンナノ粒子は膵癌治療における抗腫瘍効果を増強させる(Titanium Peroxide Nanoparticles, as Novel Radiosensitizers, Enhance Antitumor Efficacy in Pancreatic Cancer Therapy)

  Nakayama Masao, Sasaki Ryohei, Ogino Chiaki, Tanaka Tsutomu, Morita Kenta, Umetsu Mitsuo, Ohara Satoshi, Sato Kazuyoshi, Numako Chiya, Takami Seiichi, Kondo Akihiko

  (一社)日本放射線影響学会, May 2015, 日本放射線影響学会大会講演要旨集, 58回, 3 - 07, English


• Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse

  Hideo Kawaguchi, Hiroshi Teramura, Kouji Uematsu, Kiyotaka Y. Hara, Tomohisa Hasunuma, Ko Hirano, Takashi Sazuka, Hidemi Kitano, Yota Tsuge, Prihardi Kahar, Satoko Niimi-Nakamura, Ken-Ichi Oinuma, Naoki Takaya, Shigemitsu Kasuga, Chiaki Ogino, Akihiko Kondo

  Apr. 2015, BIORESOURCE TECHNOLOGY, 182, 169 - 178, English

  [Refereed][Invited]

  Scientific journal


• Mutation of arginine residues to avoid non-specific cellular uptakes for hepatitis B virus core particles

  Izzat Fahimuddin Bin Mohamed Suffian, Yuya Nishimura, Kenta Morita, Sachiko Nakamura-Tsuruta, Khuloud T. Al-Jamal, Jun Ishii, Chiaki Ogino, Akihiko Kondo

  Feb. 2015, JOURNAL OF NANOBIOTECHNOLOGY, 13(1) (1), 15, English

  [Refereed]

  Scientific journal


• Nanoparticle synthesis by biogenic approach

  Sarvesh Kumar Srivastava, Chiaki Ogino, Akihiko Kondo

  Springer International Publishing, Jan. 2015, Green Processes for Nanotechnology: From Inorganic to Bioinspired Nanomaterials, 237 - 257, English

  [Refereed]

  In book


• Saccharification and ethanol fermentation from cholinium ionic liquid-pretreated bagasse with a different number of post-pretreatment washings

  Ninomiya Kazuaki, Omote S, Ogino Chiaki, Kuroda K, Noguchi M, Endo T, Kakuchi R, Shimizu N, Takahashi K

  2015, Bioresourse Technology, 6, 888, English

  [Refereed]

  Scientific journal


• Ionic liquid/ultrasound pretreatment and in situ enzymatic saccharification of bagasse using biocompatible cholinium ionic liquid

  Kazuaki Ninomiya, Asami Kohori, Mai Tatsumi, Koji Osawa, Takatsugu Endo, Ryohei Kakuchi, Chiaki Ogino, Nobuaki Shimizu, Kenji Takahashi

  Jan. 2015, BIORESOURCE TECHNOLOGY, 176, 169 - 174, English

  [Refereed]

  Scientific journal


• Green Processes for Nanotechnology, Chapter 8, Nanoparticle Synthesis

  Srivastave S.K, Ogino Chiaki, KONDO AKIHIKO

  2015, Biogenic Approach, Springer, 237 - 257, Japanese

  Scientific journal


• Expression of cold-adapted beta-1,3-xylanase as a fusion protein with a ProS2 tag and purification using immobilized metal affinity chromatography with a high concentration of ArgHCl

  Motonori Kudou, Fumiyoshi Okazaki, Nanami Asai-Nakashima, Chiaki Ogino, Akihiko Kondo

  Jan. 2015, BIOTECHNOLOGY LETTERS, 37(1) (1), 89 - 94, English

  [Refereed]

  Scientific journal


• Characterization of fractionated biomass component and recovered ionic liquid during repeated process of cholinium ionic liquid-assisted pretreatment and fractionation

  Kazuaki Ninomiya, Ken Inoue, Yuka Aomori, Ayaka Ohnishi, Chiaki Ogino, Nobuaki Shimizu, Kenji Takahashi

  Jan. 2015, CHEMICAL ENGINEERING JOURNAL, 259, 323 - 329, English

  [Refereed]

  Scientific journal


• 二酸化チタン粒子の生物学的応用

  OGINO CHIAKI

  Jan. 2015, 化学と生物, 53(1) (1), 9 - 11, Japanese

  Scientific journal


• Expression of cold-adapted beta-1,3-xylanase as a fusion protein with a ProS2 tag and purification using immobilized metal affinity chromatography with a high concentration of ArgHCl

  Motonori Kudou, Fumiyoshi Okazaki, Nanami Asai-Nakashima, Chiaki Ogino, Akihiko Kondo

  Jan. 2015, BIOTECHNOLOGY LETTERS, 37(1) (1), 89 - 94, English

  [Refereed]

  Scientific journal


• The mapping of yeast's G-protein coupled receptor with an atomic force microscope

  Musashi Takenaka, Yusuke Miyachi, Jun Ishii, Chiaki Ogino, Akihiko Kondo

  2015, NANOSCALE, 7(11) (11), 4956 - 4963, English

  [Refereed]

  Scientific journal


• L-lactic acid production from starch by simultaneous saccharification and fermentation in a genetically engineered Aspergillus oryzae pure culture

  Satoshi Wakai, Toshihide Yoshie, Nanami Asai-Nakashima, Ryosuke Yamada, Chiaki Ogino, Hiroko Tsutsumi, Yoji Hata, Akihiko Kondo

  Dec. 2014, BIORESOURCE TECHNOLOGY, 173, 376 - 383, English

  [Refereed]

  Scientific journal


• Development of a multi-gene expression system in Xanthophyllomyces dendrorhous

  Kiyotaka Y. Hara, Toshihiko Morita, Masao Mochizuki, Keisuke Yamamoto, Chiaki Ogino, Michihiro Araki, Akihiko Kondo

  Dec. 2014, MICROBIAL CELL FACTORIES, 13(1) (1), 175, English

  [Refereed]

  Scientific journal


• Cloning and starch degradation profile of maltotriose-producing amylases from Streptomyces species

  Norimasa Kashiwagi, Michiru Miyake, Shuichi Hirose, Masahiro Sota, Chiaki Ogino, Akihiko Kondo

  Nov. 2014, BIOTECHNOLOGY LETTERS, 36(11) (11), 2311 - 2317, English

  [Refereed]

  Scientific journal


• Optimized membrane process to increase hemicellulosic ethanol production from pretreated rice straw by recombinant xylose-fermenting Saccharomyces cerevisiae

  Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Tomohisa Hasunuma, Takatoshi Sakamoto, Yuri Sakihama, Chiaki Ogino, Akihiko Kondo

  Oct. 2014, BIORESOURCE TECHNOLOGY, 169, 380 - 386, English

  [Refereed]

  Scientific journal


• Increased ethanol production from sweet sorghum juice concentrated by a membrane separation process

  Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Hiroshi Teramura, Satoshi Wakai, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

  Oct. 2014, BIORESOURCE TECHNOLOGY, 169, 821 - 825, English

  [Refereed]

  Scientific journal


• ラジカルを発生する金属酸化物ナノ粒子のX線を用いた分析

  宮嵜 世里加, 森田 健太, 荻野 千秋, 佐藤 和好, 沼子 千弥

  (公社)日本分析化学会, Sep. 2014, 日本分析化学会講演要旨集, 63年会, 168 - 168, Japanese


• Targeted sonocatalytic cancer cell injury using avidin-conjugated titanium dioxide nanoparticles

  Kazuaki Ninomiya, Aya Fukuda, Chiaki Ogino, Nobuaki Shimizu

  Sep. 2014, ULTRASONICS SONOCHEMISTRY, 21(5) (5), 1624 - 1628, English

  [Refereed]

  Scientific journal


• Microwave pretreatment of lignocellulosic material in cholinium ionic liquid for efficient enzymatic saccharification

  Kazuaki Ninomiya, Takashi Yamauchi, Chiaki Ogino, Nobuaki Shimizu, Kenji Takahashi

  Sep. 2014, BIOCHEMICAL ENGINEERING JOURNAL, 90, 90 - 95, English

  [Refereed]

  Scientific journal


• Genetic Engineering of Bio-Nanoparticles for Drug Delivery: A Review

  Yuya Nishimura, Jun Ishii, Chiaki Ogino, Akihiko Kondo

  Sep. 2014, JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 10(9) (9), 2063 - 2085, English

  [Refereed]

  Scientific journal


• Pretreatment of Japanese cedar by ionic liquid solutions in combination with acid and metal ion and its application to high solid loading

  Kazuma Ogura, Kazuaki Ninomiya, Kenji Takahashi, Chiaki Ogino, Akihiko Kondo

  Aug. 2014, BIOTECHNOLOGY FOR BIOFUELS, 7, 120, English

  [Refereed]

  Scientific journal


• The effect of combining signal sequences with the N28 fragment on GFP production in Aspergillus oryzae

  Chiak Ogino, Tetsuya Matsuda, Fumiyoshi Okazaki, Tsutomu Tanaka, Akihiko Kondo

  Jul. 2014, PROCESS BIOCHEMISTRY, 49(7) (7), 1078 - 1083, English

  [Refereed]

  Scientific journal


• Simultaneous saccharification and fermentation of kraft pulp by recombinant Escherichia coli for phenyllactic acid production

  Hideo Kawaguchi, Kouji Uematsu, Chiaki Ogino, Hiroshi Teramura, Satoko Niimi-Nakamura, Yota Tsuge, Tomohisa Hasunuma, Ken-Ichi Oinuma, Naoki Takaya, Akihiko Kondo

  Jul. 2014, BIOCHEMICAL ENGINEERING JOURNAL, 88, 188 - 194, English

  [Refereed]

  Scientific journal


• 「バイオプロダクション次世代農工連携研究」と拠点形成への展望

  KONDO AKIHIKO, OGINO CHIAKI

  テックタイムス, Jun. 2014, 紙パルプ技術タイムス, 57(6) (6), 51 - 56, Japanese

  Scientific journal


• Improvement of enzymatic activity of beta-glucosidase from Thermotoga maritima by 1-butyl-3-methylimidazolium acetate

  Motonori Kudou, Yuka Kubota, Nanami Nakashima, Fumiyoshi Okazaki, Kazunori Nakashima, Chiaki Ogino, Akihiko Kondo

  Jun. 2014, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 104, 17 - 22, English

  [Refereed]

  Scientific journal


• Enhanced OH radical generation by dual-frequency ultrasound with TiO 2 nanoparticles: Its application to targeted sonodynamic therapy

  Ninomiya, K., Noda, K., Ogino, C., Kuroda, S.-I., Shimizu, N.

  May 2014, Ultrasonics Sonochemistry, 21(1) (1), 289 - 294, English

  [Refereed]

  Scientific journal


• Aspergillus oryzae-based cell factory for direct kojic acid production from cellulose

  Ryosuke Yamada, Toshihide Yoshie, Satoshi Wakai, Nanami Asai-Nakashima, Fumiyoshi Okazaki, Chiaki Ogino, Hiromoto Hisada, Hiroko Tsutsumi, Yoji Hata, Akihiko Kondo

  May 2014, MICROBIAL CELL FACTORIES, 13, 71, English

  [Refereed]

  Scientific journal


• 植物の機能解明と新たな機能材料創出の可能性

  寺村 浩, 延田 紘治, KAWAGUCHI HIDEO, OGINO CHIAKI, NISHINO TAKASHI, KONDO AKIHIKO

  シーエムシー出版, Apr. 2014, バイオインダストリー, 31(4) (4), 62 - 68, Japanese

  Scientific journal


• Disruption of pknG enhances production of gamma-aminobutyric acid by Corynebacterium glutamicum expressing glutamate decarboxylase

  Naoko Okai, Chihiro Takahashi, Kazuki Hatada, Chiaki Ogino, Akihiko Kondo

  Apr. 2014, AMB EXPRESS, 4, 20, English

  [Refereed]

  Scientific journal


• A display of pH-sensitive fusogenic GALA peptide facilitates endosomal escape from a Bio-nanocapsule via an endocytic uptake pathway

  Yuya Nishimura, Koichi Takeda, Ryosuke Ezawa, Jun Ishii, Chiaki Ogino, Akihiko Kondo

  Apr. 2014, JOURNAL OF NANOBIOTECHNOLOGY, 12(1) (1), 11, English

  [Refereed]

  Scientific journal


• Structural evaluation of the DNA aptamer for ATP DH25.42 by AFM

  Miyachi, Y., Ogino, C., Kondo, A.

  Feb. 2014, Nucleosides Nucleotides and Nucleic Acids, 33(1) (1), 31 - 39, English

  [Refereed]

  Scientific journal


• Microbial fluorescence sensing for human neurotensin receptor type 1 using G alpha-engineered yeast cells

  Jun Ishii, Asami Oda, Shota Togawa, Akira Fukao, Toshinobu Fujiwara, Chiaki Ogino, Akihiko Kondo

  Feb. 2014, ANALYTICAL BIOCHEMISTRY, 446, 37 - 43, English

  [Refereed]

  Scientific journal


• Green synthesis of thiolated graphene nanosheets by alliin (garlic) and its effect on the deposition of gold nanoparticles

  Sarvesh Kumar Srivastava, Chiaki Ogino, Akihiko Kondo

  2014, RSC ADVANCES, 4(12) (12), 5986 - 5989, English

  [Refereed]

  Scientific journal


• Electro-catalytically active Au@Pt nanoparticles for hydrogen evolution reaction: an insight into a tryptophan mediated supramolecular interface towards a universal core-shell synthesis approach

  Sarvesh Kumar Srivastava, Jonathan Sabate del Rio, Ciara K. O'Sullivan, Chiaki Ogino, Akihiko Kondo

  2014, RSC ADVANCES, 4(89) (89), 48458 - 48464, English

  [Refereed]

  Scientific journal


• Induction of apoptosis associated with chromosomal DNA fragmentation and caspase-3 activation in leukemia L1210 cells by TiO2 nanoparticles

  Keiko Takaki, Yoshihiro Higuchi, Minako Hashii, Chiaki Ogino, Nobuaki Shimizu

  Jan. 2014, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 117(1) (1), 129 - 133, English

  [Refereed]

  Scientific journal


• Thermal Stability and Starch Degradation Profile of alpha-Amylase from Streptomyces avermitilis

  Sang Youn Hwang, Kazunori Nakashima, Naoko Okai, Fumiyoshi Okazaki, Michiru Miyake, Koichi Harazono, Chiaki Ogino, Akihiko Kondo

  Dec. 2013, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 77(12) (12), 2449 - 2453, English

  [Refereed]

  Scientific journal


• Glucose content in the liquid hydrolysate after dilute acid pretreatment is affected by the starch content in rice straw

  Hiroshi Teramura, Tomoko Oshima, Fumio Matsuda, Kengo Sasaki, Chiaki Ogino, Masanori Yamasaki, Akihiko Kondo

  Dec. 2013, BIORESOURCE TECHNOLOGY, 149, 520 - 524, English

  [Refereed]

  Scientific journal


• Particle size for photocatalytic activity of anatase TiO2nanosheets with highly exposed {001} facets

  Zhenquan Tan, Zhenquan Tan, Kazuyoshi Sato, Seiichi Takami, Chiya Numako, Mitsuo Umetsu, Kohei Soga, Masao Nakayama, Ryohei Sasaki, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo, Kazuhiro Yamamoto, Takeshi Hashishin, Satoshi Ohara

  Nov. 2013, RSC Advances, 3(42) (42), 19268 - 19271

  [Refereed]

  Scientific journal


• Ethanol fermentation by xylose-assimilating Saccharomyces cerevisiae using sugars in a rice straw liquid hydrolysate concentrated by nanofiltration

  Kengo Sasaki, Daisuke Sasaki, Yuri Sakihama, Hiroshi Teramura, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Nov. 2013, BIORESOURCE TECHNOLOGY, 147, 84 - 88, English

  [Refereed]

  Scientific journal


• ナノ粒子とX線照射を併用した深部ガンの非侵襲的治療法の開発

  田野 亜都里, 森田 健太, 荻野 千秋, 佐藤 和好, 沼子 千弥, 中山 雅央, Sasaki Ryohei, 近藤 昭彦

  日本生物工学会, Aug. 2013, 日本生物工学会大会講演要旨集平成25年度, 65, 244 - 244, Japanese

  Research society


• Ultrasonic inactivation of Microcystis aeruginosa in the presence of TiO2 particles

  Kazuaki Ninomiya, Chiaki Ogino, Shinya Kawabata, Kentaro Kitamura, Teruya Maki, Hiroshi Hasegawa, Nobuaki Shimizu

  Aug. 2013, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 116(2) (2), 214 - 218, English

  [Refereed]

  Scientific journal


• Biochemical characterization of a thermostable beta-1,3-xylanase from the hyperthermophilic eubacterium, Thermotoga neapolitana strain DSM 4359

  Fumiyoshi Okazaki, Nanami Nakashima, Chiaki Ogino, Yutaka Tamaru, Akihiko Kondo

  Aug. 2013, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(15) (15), 6749 - 6757, English

  [Refereed]

  Scientific journal


• Targeting cancer cell-specific RNA interference by siRNA delivery using a complex carrier of affibody-displaying bio-nanocapsules and liposomes

  Yuya Nishimura, Hiroaki Mieda, Jun Ishii, Chiaki Ogino, Toshinobu Fujiwara, Akihiko Kondo

  Jun. 2013, JOURNAL OF NANOBIOTECHNOLOGY, 11, 19, English

  [Refereed]

  Scientific journal


• Synergetic effect of yeast cell-surface expression of cellulase and expansin-like protein on direct ethanol production from cellulose

  Nakatani, Y., Yamada, R., Ogino, C., Kondo, A.

  Jun. 2013, Microbial Cell Factories, 12(1) (1), 66, English

  [Refereed]

  Scientific journal


• P-Hydroxycinnamic acid production directly from cellulose using endoglucanase- and tyrosine ammonia lyase-expressing Streptomyces lividans

  Yoshifumi Kawai, Shuhei Noda, Chiaki Ogino, Yasunobu Takeshima, Naoko Okai, Tsutomu Tanaka, Akihiko Kondo

  May 2013, Microbial Cell Factories, 12(1) (1), 45, English

  [Refereed]

  Scientific journal


• High-level production of mature active-form Streptomyces mobaraensis transglutaminase via pro-transglutaminase processing using Streptomyces lividans as a host

  Shuhei Noda, Takaya Miyazaki, Tsutomu Tanaka, Ogino Chiaki, Akihiko Kondo

  May 2013, Biochemical Engineering Journal, 74, 76 - 80, English

  [Refereed]

  Scientific journal


• Sidewall modification of multiwalled carbon nanotubes by Allivum sativum (garlic) and its effect on the deposition of gold nanoparticles

  Sarvesh Kumar Srivastava, Ryosuke Yamada, Chiaki Ogino, Akihiko Kondo

  May 2013, CARBON, 56, 309 - 316, English

  [Refereed]

  Scientific journal


• Production of biodiesel from plant oil hydrolysates using an Aspergillus oryzae whole-cell biocatalyst highly expressing Candida antarctica lipase B

  Daisuke Adachi, Shinji Hama, Kazunori Nakashima, Takayuki Bogaki, Chiaki Ogino, Akihiko Kondo

  May 2013, BIORESOURCE TECHNOLOGY, 135, 410 - 416, English

  [Refereed]

  Scientific journal


• Low melting point pyridinium ionic liquid pretreatment for enhancing enzymatic saccharification of cellulosic biomass

  Uju, Aya Nakamoto, Yasuhiro Shoda, Masahiro Goto, Wataru Tokuhara, Yoshiyuki Noritake, Satoshi Katahira, Nobuhiro Ishida, Chiaki Ogino, Noriho Kamiya

  May 2013, BIORESOURCE TECHNOLOGY, 135, 103 - 108, English

  [Refereed]

  Scientific journal


• A robust whole-cell biocatalyst that introduces a thermo- and solvent-tolerant lipase into Aspergillus oryzae cells: Characterization and application to enzymatic biodiesel production

  Daisuke Adachi, FookHee Koh, Shinji Hama, Chiaki Ogino, Akihiko Kondo

  May 2013, ENZYME AND MICROBIAL TECHNOLOGY, 52(6-7) (6-7), 331 - 335, English

  [Refereed]

  Scientific journal


• Inactivation of Escherichia coli by sonoelectrocatalytic disinfection using TiO2 as electrode

  Kazuaki Ninomiya, Masayuki Arakawa, Chiaki Ogino, Nobuaki Shimizu

  Mar. 2013, ULTRASONICS SONOCHEMISTRY, 20(2) (2), 762 - 767, English

  [Refereed]

  Scientific journal


• Granting specificity for breast cancer cells using a hepatitis B core particle with a HER2-targeted affibody molecule

  Yuya Nishimura, Wakiko Mimura, Izzat Fahimuddin Mohamed Suffian, Tomokazu Amino, Jun Ishii, Chiaki Ogino, Akihiko Kondo

  Mar. 2013, JOURNAL OF BIOCHEMISTRY, 153(3) (3), 251 - 256, English

  [Refereed]

  Scientific journal


• Cell-SELEX based selection and characterization of DNA aptamer recognizing human hepatocarcinoma

  Kazuaki Ninomiya, Kazuhiko Kaneda, Satoshi Kawashima, Yusuke Miyachi, Chiaki Ogino, Nobuaki Shimizu

  Mar. 2013, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 23(6) (6), 1797 - 1802, English

  [Refereed]

  Scientific journal


• An affinity chromatography method used to purify His-tag-displaying bio-nanocapsules

  Nishimura, Y., Takeda, K., Ishii, J., Ogino, C., Kondo, A.

  Mar. 2013, Journal of Virological Methods, 189(2) (2), 393 - 396, English

  [Refereed]

  Scientific journal


• Cholinium carboxylate ionic liquids for pretreatment of lignocellulosic materials to enhance subsequent enzymatic saccharification

  Kazuaki Ninomiya, Takashi Yamauchi, Masafumi Kobayashi, Chiaki Ogino, Nobuaki Shimizu, Kenji Takahashi

  Feb. 2013, Biochemical Engineering Journal, 71, 25 - 29, English

  Scientific journal


• イオン液体前処理バイオマスを用いたエタノール同時糖化発酵

  OGINO CHIAKI, YAMADA RYOSUKE, KONDO AKIHIKO

  Feb. 2013, 化学工業, 64(2) (2), 52 - 55, Japanese

  Scientific journal


• Image analyzing method to evaluate in situ bioluminescence from an obligate anaerobe cultivated under various dissolved oxygen concentrations

  Kazuaki Ninomiya, Ryuji Yamada, Masami Matsumoto, Satoru Fukiya, Takane Katayama, Chiaki Ogino, Nobuaki Shimizu

  Feb. 2013, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 115(2) (2), 196 - 199, English

  [Refereed]

  Scientific journal


• Biogenic synthesis and characterization of gold nanoparticles by Escherichia coli K12 and its heterogeneous catalysis in degradation of 4-nitrophenol

  Sarvesh Kumar Srivastava, Ryosuke Yamada, Chiaki Ogino, Akihiko Kondo

  Feb. 2013, NANOSCALE RESEARCH LETTERS, 8(1) (1), 70, English

  [Refereed]

  Scientific journal


• An integrative process model of enzymatic biodiesel production through ethanol fermentation of brown rice followed by lipase-catalyzed ethanolysis in a water-containing system

  Daisuke Adachi, Risa Koda, Shinji Hama, Ryosuke Yamada, Kazunori Nakashima, Chiaki Ogino, Akihiko Kondo

  Feb. 2013, ENZYME AND MICROBIAL TECHNOLOGY, 52(2) (2), 118 - 122, English

  [Refereed]

  Scientific journal


• Ability of a perfluoropolymer membrane to tolerate by-products of ethanol fermentation broth from dilute acid-pretreated rice straw

  Kengo Sasaki, Fumio Matsuda, Tomohisa Hasunuma, Chiaki Ogino, Masakatsu Urairi, Kazuhito Yoshida, Akihiko Kondo

  Jan. 2013, Biochemical Engineering Journal, 70, 135 - 139, English

  [Refereed]

  Scientific journal


• Combined use of completely bio-derived cholinium ionic liquids and ultrasound irradiation for the pretreatment of lignocellulosic material to enhance enzymatic saccharification

  Kazuaki Ninomiya, Akiko Ohta, Sayuri Omote, Chiaki Ogino, Kenji Takahashi, Nobuaki Shimizu

  Jan. 2013, Chemical Engineering Journal, 215-216, 811 - 818, English

  Scientific journal


• Green synthesis of Au, Pd and Au@ Pd core-shell nanoparticles via a tryptophan induced supramolecular interface

  Sarvesh Kumar Srivastava, Takashi Hasegawa, Ryosuke Yamada, Chiaki Ogino, Minoru Mizuhata, Akihiko Kondo

  2013, RSC ADVANCES, 3(40) (40), 18367 - 18372, English

  [Refereed]

  Scientific journal


• Effect of ionic liquid weight ratio on pretreatment of bamboo powder prior to enzymatic saccharification

  Kazuaki Ninomiya, Hiroshi Soda, Chiaki Ogino, Kenji Takahashi, Nobuaki Shimizu

  Jan. 2013, BIORESOURCE TECHNOLOGY, 128, 188 - 192, English

  [Refereed]

  Scientific journal


• Efficient direct ethanol production from cellulose by cellulase- and cellodextrin transporter-co-expressing Saccharomyces cerevisiae

  Ryosuke Yamada, Yuki Nakatani, Chiaki Ogino, Akihiko Kondo

  Springer Verlag, 2013, AMB Express, 3(1) (1), 1 - 7, English

  [Refereed]

  Scientific journal


• Complex carriers of affibody-displaying bio-nanocapsules and composition-varied liposomes for HER2-expressing breast cancer cell-specific protein delivery

  Yuya Nishimura, Jun Ishii, Fumiyoshi Okazaki, Chiaki Ogino, Akihiko Kondo

  Dec. 2012, JOURNAL OF DRUG TARGETING, 20(10) (10), 897 - 905, English

  [Refereed]

  Scientific journal


• Efficient heterologous expression and secretion in Aspergillus oryzae of a llama variable heavy-chain antibody fragment V-HH against EGFR

  Fumiyoshi Okazaki, Jun-ichi Aoki, Soichiro Tabuchi, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Oct. 2012, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 96(1) (1), 81 - 88, English

  [Refereed]

  Scientific journal


• Sugar consumption and ethanol fermentation by transporter-overexpressed xylose-metabolizing Saccharomyces cerevisiae harboring a xyloseisomerase pathway

  Takanori Tanino, Tomonori Ito, Chiaki Ogino, Naoto Ohmura, Takayuki Ohshima, Akihiko Kondo

  Aug. 2012, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 114(2) (2), 209 - 211, English

  [Refereed]

  Scientific journal


• Recent developments in yeast cell surface display toward extended applications in biotechnology

  Tsutomu Tanaka, Ryosuke Yamada, Chiaki Ogino, Akihiko Kondo

  Aug. 2012, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 95(3) (3), 577 - 591, English

  [Refereed]

  Scientific journal


• Robust production of gamma-amino butyric acid using recombinant Corynebacterium glutamicum expressing glutamate decarboxylase from Escherichia coli

  Chihiro Takahashi, Junki Shirakawa, Takeyuki Tsuchidate, Naoko Okai, Kazuki Hatada, Hideki Nakayama, Toshihiro Tateno, Chiaki Ogino, Akihiko Kondo

  Aug. 2012, ENZYME AND MICROBIAL TECHNOLOGY, 51(3) (3), 171 - 176, English

  [Refereed]

  Scientific journal


• Direct isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display system

  Yuki Soma, Kentaro Inokuma, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo, Masahiro Okamoto, Taizo Hanai

  Jul. 2012, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 114(1) (1), 80 - 85, English

  [Refereed]

  Scientific journal


• Repeated fermentation from raw starch using Saccharomyces cerevisiae displaying both glucoamylase and α-amylase

  Syun-Ichi Yamakawa, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  May 2012, Enzyme and Microbial Technology, 50(6-7) (6-7), 343 - 347, English

  [Refereed]

  Scientific journal


• イオン液体と超音波照射を組み合わせたリグノセルロース前処理によるバイオマス・リファイナリー

  高橋 憲司, 仁宮 一章, OGINO CHIAKI, 清水 宣明

  May 2012, バイオインダストリー, 29, 38 - 45, Japanese

  Scientific journal


• Targeted sonodynamic therapy using protein-modified TiO2 nanoparticles

  Kazuaki Ninomiya, Chiaki Ogino, Shuhei Oshima, Shiro Sonoke, Shun-ichi Kuroda, Nobuaki Shimizu

  May 2012, ULTRASONICS SONOCHEMISTRY, 19(3) (3), 607 - 614, English

  [Refereed]

  Scientific journal


• Genetic engineering to enhance the Ehrlich pathway and alter carbon flux for increased isobutanol production from glucose by Saccharomyces cerevisiae

  Takashi Kondo, Hironori Tezuka, Jun Ishii, Fumio Matsuda, Chiaki Ogino, Akihiko Kondo

  May 2012, JOURNAL OF BIOTECHNOLOGY, 159(1-2) (1-2), 32 - 37, English

  [Refereed]

  Scientific journal


• Cell Wall Trapping of Autocrine Peptides for Human G-Protein-Coupled Receptors on the Yeast Cell Surface

  Jun Ishii, Nobuo Yoshimoto, Kenji Tatematsu, Shun'ichi Kuroda, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  May 2012, PLOS ONE, 7(5) (5), e37136, English

  [Refereed]

  Scientific journal


• An energy-saving glutathione production method from low-temperature cooked rice using amylase-expressing Saccharomyces cerevisiae

  Kiyotaka Y. Hara, Songhee Kim, Kentaro Kiriyama, Hideyo Yoshida, Shogo Arai, Jun Ishii, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  May 2012, BIOTECHNOLOGY JOURNAL, 7(5) (5), 686 - 689, English

  [Refereed]

  Scientific journal


• A novel prevention method against re-obstruction of titanium alloy stent for biliary malignancy using generation of hydroxyl radical under ultrasonic irradiation

  Tomonori Fujimura, Osamu Itano, Sachiko Matsuda, Onishi Tatsuya, Minoru Kitago, Masahiro Shinoda, Shigeyuki Kawachi, Minoru Tanabe, Chiaki Ogino, Nobuaki Shimizu, Yuko Kitagawa

  Apr. 2012, CANCER RESEARCH, 72, English

  [Refereed]


• A novel sonodynamic therapy using hydroxyl radical generated from ultrasound activated TiO2 for human epithelial carcinoma cells

  Osamu Itano, Tatsuya Onishi, Sachiko Matsuda, Tomonori Fujimura, Hiromitsu Jinno, Minoru Kitagou, Masahiro Shinoda, Shigeyuki Kawachi, Minoru Tanabe, Chiaki Ogino, Nobuaki Shimizu, Yuko Katagawa

  Apr. 2012, CANCER RESEARCH, 72, English


• Benzoic acid fermentation from starch and cellulose via a plant-like beta-oxidation pathway in Streptomyces maritimus

  Shuhei Noda, Eiichi Kitazono, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Apr. 2012, MICROBIAL CELL FACTORIES, 11(1) (1), 49, English

  [Refereed]

  Scientific journal


• 酸化チタン粒子のバイオ応用

  OGINO CHIAKI

  日本生物工学会, Mar. 2012, 生物工学会誌, 90(3) (3), 110 - 114, Japanese

  Scientific journal


• Kinetic characterization and Mg2+ enhancement of Streptomyces griseocarneus sphingomyelinase C produced by recombinant Streptomyces lividans

  Daisuke Sugimori, Yusaku Matsumoto, Yu Tomita, Kazutaka Murayama, Chiaki Ogino

  Feb. 2012, PROTEIN EXPRESSION AND PURIFICATION, 81(2) (2), 151 - 156, English

  [Refereed]

  Scientific journal


• Development of a glutathione production process from proteinaceous biomass resources using protease-displaying Saccharomyces cerevisiae

  Kiyotaka Y. Hara, Songhee Kim, Hideyo Yoshida, Kentaro Kiriyama, Takashi Kondo, Naoko Okai, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Feb. 2012, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 93(4) (4), 1495 - 1502, English

  [Refereed]

  Scientific journal


• Multidisciplinary studies of inorganic nanoparticles and biomolecules for cell injury

  Ogino, C.

  2012, Seibutsu Kogaku Kaishi, 90(3) (3)

  Scientific journal


• Short time ionic liquids pretreatment on lignocellulosic biomass to enhance enzymatic saccharification

  Uju, Yasuhiro Shoda, Aya Nakamoto, Masahiro Goto, Wataru Tokuhara, Yoshiyuki Noritake, Satoshi Katahira, Nobuhiro Ishida, Kazunori Nakashima, Chiaki Ogino, Noriho Kamiya

  Jan. 2012, BIORESOURCE TECHNOLOGY, 103(1) (1), 446 - 452, English

  [Refereed]

  Scientific journal


• Protein-encapsulated bio-nanocapsules production with ER membrane localization sequences

  Yuya Nishimura, Takuya Shishido, Jun Ishii, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Jan. 2012, JOURNAL OF BIOTECHNOLOGY, 157(1) (1), 124 - 129, English

  [Refereed]

  Scientific journal


• Production of Streptoverticillium cinnamoneum transglutaminase and cinnamic acid by recombinant Streptomyces lividans cultured on biomass-derived carbon sources

  Shuhei Noda, Takaya Miyazaki, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Jan. 2012, BIORESOURCE TECHNOLOGY, 104, 648 - 651, English

  [Refereed]

  Scientific journal


• Development of a novel aptamer-based sensing system using atomic force microscopy

  Yusuke Miyachi, Chiaki Ogino, Tomokazu Amino, Akihiko Kondo

  Nov. 2011, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 112(5) (5), 511 - 514, English

  [Refereed]

  Scientific journal


• Homo-D-lactic acid production from mixed sugars using xylose-assimilating operon-integrated Lactobacillus plantarum

  Shogo Yoshida, Kenji Okano, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Oct. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 92(1) (1), 67 - 76, English

  [Refereed]

  Scientific journal


• Improved homo l-lactic acid fermentation from xylose by abolishment of the phosphoketolase pathway and enhancement of the pentose phosphate pathway in genetically modified xylose-assimilating Lactococcus lactis

  Satoru Shinkawa, Kenji Okano, Shogo Yoshida, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Sep. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 91(6) (6), 1537 - 1544, English

  [Refereed]

  Scientific journal


• Creation of a Cellooligosaccharide-Assimilating Escherichia coli Strain by Displaying Active Beta-Glucosidase on the Cell Surface via a Novel Anchor Protein

  Tsutomu Tanaka, Hitomi Kawabata, Chiaki Ogino, Akihiko Kondo

  Sep. 2011, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77(17) (17), 6265 - 6270, English

  [Refereed]

  Scientific journal


• Variation in Biomass Properties among Rice Diverse Cultivars

  Fumio Matsuda, Masanori Yamasaki, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Aug. 2011, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 75(8) (8), 1603 - 1605, English

  [Refereed]

  Scientific journal


• Enzymatic glutathione production using metabolically engineered Saccharomyces cerevisiae as a whole-cell biocatalyst

  Hideyo Yoshida, Kiyotaka Y. Hara, Kentaro Kiriyama, Hideki Nakayama, Fumiyoshi Okazaki, Fumio Matsuda, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Aug. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 91(4) (4), 1001 - 1006, English

  [Refereed]

  Scientific journal


• Expression, crystallization and preliminary X-ray diffraction studies of thermostable beta-1,3-xylanase from Thermotoga neapolitana strain DSM 4359

  Fumiyoshi Okazaki, Chiaki Ogino, Akihiko Kondo, Bunzo Mikami, Yoichi Kurebayashi, Hiroki Tsuruta

  Jul. 2011, ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 67, 779 - 781, English

  [Refereed]

  Scientific journal


• Development of an Aspergillus oryzae whole-cell biocatalyst coexpressing triglyceride and partial glyceride lipases for biodiesel production

  Daisuke Adachi, Shinji Hama, Takao Numata, Kazunori Nakashima, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Jun. 2011, BIORESOURCE TECHNOLOGY, 102(12) (12), 6723 - 6729, English

  [Refereed]

  Scientific journal


• Highly efficient biodiesel production by a whole-cell biocatalyst employing a system with high lipase expression in Aspergillus oryzae

  Tomohiro Takaya, Risa Koda, Daisuke Adachi, Kazunori Nakashima, Junpei Wada, Takayuki Bogaki, Chiaki Ogino, Akihiko Kondo

  May 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 90(3) (3), 1171 - 1177, English

  [Refereed]

  Scientific journal


• Glutamate production from beta-glucan using endoglucanase-secreting Corynebacterium glutamicum

  Takeyuki Tsuchidate, Toshihiro Tateno, Naoko Okai, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  May 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 90(3) (3), 895 - 901, English

  [Refereed]

  Scientific journal


• Cinnamic acid production using Streptomyces lividans expressing phenylalanine ammonia lyase

  Shuhei Noda, Takaya Miyazaki, Takanori Miyoshi, Michiru Miyake, Naoko Okai, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  May 2011, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 38(5) (5), 643 - 648, English

  [Refereed]

  Scientific journal


• Direct and efficient ethanol production from high-yielding rice using a Saccharomyces cerevisiae strain that express amylases

  Ryosuke Yamada, Syun-ichi Yamakawa, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Apr. 2011, ENZYME AND MICROBIAL TECHNOLOGY, 48(4-5) (4-5), 393 - 396, English

  [Refereed]

  Scientific journal


• Extracellular production of a sphingomyelinase fromStreptomyces griseocarneus using Streptomyces lividans

  SUGIMORI, D, TOMITA, Y, MATSUMOTO, Y, OGINO Chiaki

  Mar. 2011, Biotechnology Letters, 33(4), 727-731, 727 - 731, English

  [Refereed]

  Scientific journal


• Efficient and direct glutathione production from raw starch using engineered Saccharomyces cerevisiae

  Hideyo Yoshida, Shogo Arai, Kiyotaka Y. Hara, Ryosuke Yamada, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Mar. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 89(5) (5), 1417 - 1422, English

  [Refereed]

  Scientific journal


• S28. Targeted sonodynamic therapy using protein-modified TiO_2 nanoparticles(Oral Presentation)

  Ninomiya Kazuaki, Oshima Shuhei, Sonoke Shiro, Ogino Chiaki, Kuroda Shun-ichi, Shimizu Nobuaki

  Our previous study suggested new sonodynamic therapy for cancer cells based on the delivery of titanium dioxide (TiO_2) nanoparticles (NPs) modified with a protein specifically recognizing target cells and subsequent generation of hydroxyl radicals from TiO_2 NPs activated by external ultrasound irradiation (called TiO_2/US treatment). The present study first examined the uptake behavior of TiO_2 NPs modified with pre-S1/S2 (model protein-recognizing hepatocytes) by HepG2 cells for 24 h. Next, the effect of the TiO_2/US treatment on HepG2 cell growth was examined for 96 h after the 1 MHz ultrasound was irradiated (0.1 W/cm^2, 30 s) to the cells which incorporated the TiO_2 NPs. Although no apparent cell-injury was observed until 24 h after the treatment, the viable cell concentration had deteriorated to 46% of the control at 96 h. Finally, the TiO_2/US treatment was applied to a mouse xenograft model. The pre-S1/S2-immobilized TiO_2 (0.1 mg) was directly injected into tumors, followed by 1 MHz ultrasound irradiation at 1.0 W/cm^2 for 60 s. As a result of the treatment repeated 5 times within 13 days, tumor growth could be hampered up to 28 days compared with the control conditions.

  The Japan Society of Sonochemistry, 2011, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 20, 80 - 83, English


• Aromatic chemicals production using phenylalnine ammonia lyase expressing Streptomyces lividans

  Chiaki Ogino, Shuhei Noda, Akihiko Kondo, Naoko Okai, Tsutomu Tanaka

  2011, 2011 Defense Science Research Conference and Expo, DSR 2011, English

  [Refereed]

  International conference proceedings


• Direct ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiae with optimized cellulase expression

  Ryosuke Yamada, Naho Taniguchi, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  BioMed Central Ltd., 2011, Biotechnology for Biofuels, 4(1) (1), 8, English

  [Refereed]

  Scientific journal


• Direct ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiae with optimized cellulase expression

  YAMADA Ryosuke, TANIGUCHI N, TANAKA Tsutomu, OGINO Chiaki, FUKUDA Hideki, KONDO Akihiko

  2011, Biotechnology for Biofuels, 4:8, English

  [Refereed]

  Scientific journal


• Direct bioethanol production from cellulose by the combination of cellulase-displaying yeast and ionic liquid pretreatment

  Kazunori Nakashima, Koji Yamaguchi, Naho Taniguchi, Shogo Arai, Ryosuke Yamada, Satoshi Katahira, Nobuhiro Ishida, Haruo Takahashi, Chiaki Ogino, Akihiko Kondo

  2011, GREEN CHEMISTRY, 13(10) (10), 2948 - 2953, English

  [Refereed]

  Scientific journal


• Construction of a xylose-metabolizing yeast by genome integration of xylose isomerase gene and investigation of the effect of xylitol on fermentation

  Takanori Tanino, Atsushi Hotta, Tomonori Ito, Jun Ishii, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Naoto Ohmura, Takayuki Ohshima, Akihiko Kondo

  Nov. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 88(5) (5), 1215 - 1221, English

  [Refereed]

  Scientific journal


• Construction of a xylose-metabolizing Saccharomyces cerevisiae by integration of xylose isomerase gene into the genome and investigation of the effect of xylITOl on fermentation

  Takanori Tanino, Atsushi Hotta, Tomonori Ito, Jun Ishii, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Naoto Ohmura, Takayuki Ohima, Akihiko Kondo

  Nov. 2010, Applied Microbiology and Biotechnology, 88, 1215-1221, English

  [Refereed]

  Scientific journal


• Over-production of various secretory-form proteins in Streptomyces lividans

  Shuhei Noda, Yuko Ito, Nobuaki Shimizu, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Oct. 2010, PROTEIN EXPRESSION AND PURIFICATION, 73(2) (2), 198 - 202, English

  [Refereed]

  Scientific journal


• Affibody-displaying bionanocapsules for specific drug delivery to HER2-expressing cancer cells

  Takuya Shishido, Hiroaki Mieda, Sang Youn Hwang, Yuya Nishimura, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Oct. 2010, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 20(19) (19), 5726 - 5731, English

  [Refereed]

  Scientific journal


• Ethanolysis of rapeseed oil to produce biodiesel fuel catalyzed by Fusarium heterosporum lipase-expressing fungus immobilized whole-cell biocatalysts

  Risa Koda, Takao Numata, Shinji Hama, Sriappareddy Tamalampudi, Kazunori Nakashima, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Sep. 2010, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 66(1-2) (1-2), 101 - 104, English

  [Refereed]

  Scientific journal


• Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes

  Shuhei Yanase, Tomohisa Hasunuma, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Sep. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 88(1) (1), 381 - 388, English

  [Refereed]

  Scientific journal


• Construction of protein-modified TiO2 nanoparticles for use with ultrasound irradiation in a novel cell injuring method

  Chiaki Ogino, Naonori Shibata, Ryosuke Sasai, Keiko Takaki, Yusuke Miyachi, Shun-ichi Kuroda, Kazuaki Ninomiya, Nobuaki Shimizu

  Sep. 2010, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 20(17) (17), 5320 - 5325, English

  [Refereed]

  Scientific journal


• Biofunctional TiO2 nanoparticle-mediated photokilling of cancer cells using UV irradiation

  Kazusa Matsui, Miki Karasaki, Maiko Segawa, Sang Youn Hwang, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Sep. 2010, MEDCHEMCOMM, 1(3) (3), 209 - 211, English

  [Refereed]

  Scientific journal


• Display of both N- and C-terminal target fusion proteins on the Aspergillus oryzae cell surface using a chitin-binding module

  Soichiro Tabuchi, Junji Ito, Takashi Adachi, Hiroki Ishida, Yoji Hata, Fumiyoshi Okazaki, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Aug. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(5) (5), 1783 - 1789, English

  [Refereed]

  Scientific journal


• Repeated batch fermentation from raw starch using a maltose transporter and amylase expressing diploid yeast strain

  Syun-ichi Yamakawa, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Jun. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(1) (1), 109 - 115, English

  [Refereed]

  Scientific journal


• Importance of asparagine residues at positions 13 and 26 on the amino-terminal domain of human somatostatin receptor subtype-5 in signalling

  Shota Togawa, Jun Ishii, Atsushi Ishikura, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Jun. 2010, JOURNAL OF BIOCHEMISTRY, 147(6) (6), 867 - 873, English

  [Refereed]

  Scientific journal


• Control of signalling properties of human somatostatin receptor subtype-5 by additional signal sequences on its amino-terminus in yeast

  Yusuke Iguchi, Jun Ishii, Hideki Nakayama, Atsushi Ishikura, Keiko Izawa, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Jun. 2010, JOURNAL OF BIOCHEMISTRY, 147(6) (6), 875 - 884, English

  [Refereed]

  Scientific journal


• Ethanol production from cellulosic materials using cellulase-expressing yeast

  Shuhei Yanase, Ryosuke Yamada, Shohei Kaneko, Hideo Noda, Tomohisa Hasunuma, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  May 2010, BIOTECHNOLOGY JOURNAL, 5(5) (5), 449 - 455, English

  [Refereed]

  Scientific journal


• Cocktail delta-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains

  Ryosuke Yamada, Naho Taniguchi, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  May 2010, MICROBIAL CELL FACTORIES, 9, English

  [Refereed]

  Scientific journal


• Ultrasound-induced membrane lipid peroxidation and cell damage of Escherichia coli in the presence of non-woven TiO2 fabrics

  Mohammad Mizanur Rahman, Kazuaki Ninomiya, Chiaki Ogino, Nobuaki Shimizu

  Apr. 2010, ULTRASONICS SONOCHEMISTRY, 17(4) (4), 738 - 743, English

  [Refereed]

  Scientific journal


• Selection of DNA aptamers using atomic force microscopy

  Yusuke Miyachi, Nobuaki Shimizu, Chiaki Ogino, Akihiko Kondo

  Mar. 2010, NUCLEIC ACIDS RESEARCH, 38(4) (4), English

  [Refereed]

  Scientific journal


• Fatty acid production from butter using novel cutinase-displaying yeast

  Ken Horii, Takashi Adachi, Takanori Tanino, Tsutomu Tanaka, Atsushi Kotaka, Hiroshi Sahara, Tsuneharu Hashimoto, Nobuyuki Kuratani, Seiji Shibasaki, Chiaki Ogino, Hideo Noda, Yoji Hata, Mitsuyoshi Ueda, Akihiko Kondo

  Mar. 2010, ENZYME AND MICROBIAL TECHNOLOGY, 46(3-4) (3-4), 194 - 199, English

  [Refereed]

  Scientific journal


• Potential uses of titanium dioxide in conjunction with ultrasound for improved disinfection

  Nobuaki Shimizu, Kazuaki Ninomiya, Chiaki Ogino, Mohammad Mizanur Rahman

  Feb. 2010, Biochemical Engineering Journal, 48(3) (3), 416 - 423, English

  [Refereed]

  Scientific journal


• Novel strategy for yeast construction using delta-integration and cell fusion to efficiently produce ethanol from raw starch

  Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Feb. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85(5) (5), 1491 - 1498, English

  [Refereed]

  Scientific journal


• 高効率バイオディーゼル生産を目的としたWhole-cell biocatalystによる新規リパーゼ複合系の開発

  足立 大輔, 中島 一紀, 荻野 千秋, 近藤 昭彦

  公益社団法人 化学工学会, 2010, 化学工学会 研究発表講演要旨集, 2010, 1008 - 1008, Japanese


• 力学的指標による生細胞表面におけるリガンド‐受容体の相互作用解析

  野坂 和輝, 宮地 佑典, 石井 純, 荻野 千秋, 近藤 昭彦

  公益社団法人 化学工学会, 2010, 化学工学会 研究発表講演要旨集, 2010, 983 - 983, Japanese


• affibody提示バイオナノカプセルを用いたタンパク質デリバリーシステム

  西村 勇哉, 三枝 宏彰, 田中 勉, 荻野 千秋, 近藤 昭彦

  公益社団法人 化学工学会, 2010, 化学工学会 研究発表講演要旨集, 2010, 963 - 963, Japanese


• 機能性酵母によるバイオエタノール生産プロセスの強化

  伊藤 友教, 内田 敦子, 堀田 淳史, 堀江 孝史, 荻野 千秋, 大村 直人

  公益社団法人 化学工学会, 2010, 化学工学会 研究発表講演要旨集, 2010, 9 - 9, Japanese


• 酵母によるエタノール発酵に及ぼす撹拌剪断効果

  内田 敦子, 伊藤 友教, 堀田 淳史, 堀江 孝史, 荻野 千秋, 大村 直人

  公益社団法人 化学工学会, 2010, 化学工学会 研究発表講演要旨集, 2010, 774 - 774, Japanese


• Intensification of bio-ethanol fermentation by recombinant yeast with xylose isomerase pathway

  Tomonori Ito, Atsushi Hotta, Atsuko Uchida, Takanori Tanino, Chiaki Ogino, Akihiko Kondo, Naoto Ohmura

  Italian Association of Chemical Engineering - AIDIC, 2010, Chemical Engineering Transactions, 20, 103 - 108, English

  [Refereed]

  International conference proceedings


• Intensification of Bio-ethanol Fermentation by Recombinant Yeast with Xylose Isomerase Pathway

  Tomonori Ito, Atsushi Hotta, Atsuko Uchida, Takanori Tanino, Chiaki Ogino, Akihiko Kondo, Naoto Ohmura

  2010, IBIC2010: 2ND INTERNATIONAL CONFERENCE ON INDUSTRIAL BIOTECHNOLOGY, 20, 103 - 108, English

  [Refereed]

  International conference proceedings


• Production of biodiesel fuel from soybean oil catalyzed by fungus whole-cell biocatalysts in ionic liquids

  Shogo Arai, Kazunori Nakashima, Takanori Tanino, Chiaki Ogino, Akihiko Kondo, Hideki Fukuda

  Jan. 2010, ENZYME AND MICROBIAL TECHNOLOGY, 46(1) (1), 51 - 55, English

  [Refereed]

  Scientific journal


• D-lactic acid production from cellooligosaccharides and beta-glucan using L-LDH gene-deficient and endoglucanase-secreting Lactobacillus plantarum

  Kenji Okano, Qiao Zhang, Shogo Yoshida, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Jan. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85(3) (3), 643 - 650, English

  [Refereed]

  Scientific journal


• Biotechnological production of enantiomeric pure lactic acid from renewable resources: recent achievements, perspectives, and limits

  Kenji Okano, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Jan. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85(3) (3), 413 - 423, English

  [Refereed]

  Scientific journal


• Improved Production of Homo-D-Lactic Acid via Xylose Fermentation by Introduction of Xylose Assimilation Genes and Redirection of the Phosphoketolase Pathway to the Pentose Phosphate Pathway in L-Lactate Dehydrogenase Gene-Deficient Lactobacillus plantarum

  Kenji Okano, Shogo Yoshida, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Dec. 2009, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(24) (24), 7858 - 7861, English

  [Refereed]

  Scientific journal


• Biotinylated Bionanocapsules for Displaying Diverse Ligands Toward Cell-specific Delivery

  Takuya Shishido, Yuki Azumi, Takeshi Nakanishi, Mitsuo Umetsu, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Dec. 2009, JOURNAL OF BIOCHEMISTRY, 146(6) (6), 867 - 874, English

  [Refereed]

  Scientific journal


• Ester synthesis reaction with CALB displaying yeast whole cell biocatalyst: Effect of organic solvent and initial water content

  Takanori Tanino, Takumi Ohm, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(5) (5), 369 - 371, English

  [Refereed]

  Scientific journal


• Homo-D-Lactic Acid Fermentation from Arabinose by Redirection of the Phosphoketolase Pathway to the Pentose Phosphate Pathway in L-Lactate Dehydrogenase Gene-Deficient Lactobacillus plantarum

  Kenji Okano, Shogo Yoshida, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Aug. 2009, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(15) (15), 5175 - 5178, English

  [Refereed]

  Scientific journal


• Improvement of isoflavone aglycones production using beta-glucosidase secretory produced in recombinant Aspergillus oryzae

  Ken Horii, Takashi Adachi, Tetsuya Matsuda, Tsutomu Tanaka, Hiroshi Sahara, Seiji Shibasaki, Chiaki Ogino, Yoji Hata, Mitsuyoshi Ueda, Akihiko Kondo

  Aug. 2009, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 59(4) (4), 297 - 301, English

  [Refereed]

  Scientific journal


• Evaluation of cell surface-displayed protein stability against simulated gastric fluid

  Ken Horii, Takashi Adachi, Takanori Tanino, Tsutomu Tanaka, Hiroshi Sahara, Seiji Shibasaki, Chiaki Ogino, Yoji Hata, Mitsuyoshi Ueda, Akihiko Kondo

  Aug. 2009, BIOTECHNOLOGY LETTERS, 31(8) (8), 1259 - 1264, English

  [Refereed]

  Scientific journal


• Selection of a DNA aptamer that binds 8-OHdG using GMP-agarose

  Yusuke Miyachi, Nobuaki Shimizu, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Jul. 2009, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 19(13) (13), 3619 - 3622, English

  [Refereed]

  Scientific journal


• Regulation of the Display Ratio of Enzymes on the Saccharomyces cerevisiae Cell Surface by the Immunoglobulin G and Cellulosomal Enzyme Binding Domains

  Junji Ito, Akihiko Kosugi, Tsutomu Tanaka, Kouichi Kuroda, Seiji Shibasaki, Chiaki Ogino, Mitsuyoshi Ueda, Hideki Fukuda, Roy H. Doi, Akihiko Kondo

  Jun. 2009, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(12) (12), 4149 - 4154, English

  [Refereed]

  Scientific journal


• A Simple and Immediate Method for Simultaneously Evaluating Expression Level and Plasmid Maintenance in Yeast

  Jun Ishii, Keiko Izawa, Shizuka Matsumura, Kanako Wakamura, Takanori Tanino, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Jun. 2009, JOURNAL OF BIOCHEMISTRY, 145(6) (6), 701 - 708, English

  [Refereed]

  Scientific journal


• Efficient production of ethanol from raw starch by a mated diploid Saccharomyces cerevisiae with integrated alpha-amylase and glucoamylase genes

  Ryosuke Yamada, Yohei Bito, Takashi Adachi, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  May 2009, ENZYME AND MICROBIAL TECHNOLOGY, 44(5) (5), 344 - 349, English

  [Refereed]

  Scientific journal


• Construction of arginine-rich peptide displaying bionanocapsules

  Takuya Shishido, Daisaku Yonezawa, Kiyokazu Iwata, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Mar. 2009, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 19(5) (5), 1473 - 1476, English

  [Refereed]

  Scientific journal


• Improvement of a Candida antarctica lipase B-displaying yeast whole-cell biocatalyst and its application to the polyester synthesis reaction

  Takanori Tanino, Tohru Aoki, Won-young Chung, Yuka Watanabe, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Feb. 2009, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 82(1) (1), 59 - 66, English

  [Refereed]

  Scientific journal


• TiO&2&ナノ粒子と超音波を併用した新規がん治療法構築のための基礎的検討

  仁宮 一章, 園家 史朗, 大島 周平, 荻野 千秋, 黒田 俊一, 清水 宣明

  公益社団法人 化学工学会, 2009, 化学工学会 研究発表講演要旨集, 2009, 728 - 728, Japanese


• 非侵襲性がん治療のためのタンパク質修飾ナノ粒子の開発

  松井 かずさ, 荻野 千秋, 田中 勉, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2009, 化学工学会 研究発表講演要旨集, 2009, 727 - 727, Japanese


• 放線菌遺伝子組み換え系のタンパク質分泌生産への適用

  荻野 千秋, 伊藤 裕子, 宮下 徹, 仁宮 一章, 清水 宣明

  公益社団法人 化学工学会, 2009, 化学工学会 研究発表講演要旨集, 2009, 601 - 601, Japanese


• Bioethanol Production from Cellulose Material by Arming Yeast(New Midori Revolution-Seeking after a dream energy-)

  Ogino Chiaki, Kondo Akihiko

  平成14年度に日本政府の総合戦略「バイオマス・ニッポン」が策定され、バイオマスの有効利用による持続的に発展可能な社会の実現が提言されている。この戦略は、（1）バイオマスの有効利用に基づく地球温暖化防止や循環型社会形成の達成、（2）日本独自のバイオマス利用法の開発による戦略的産業の育成を目指すものである。また、地球規模での環境保護の観点から、バイオマス原料は日本のみならず、世界中から安価かつ豊富な資源の積極的な利用が求められている。さらに石油資源枯渇や価格高騰の影響より、これまでの石油資源依存型の「オイルリファイナリー」から、バイオマスベースの「バイオリファイナリー」社会への転換が急務とされている。米国においても、1999年にクリントン大統領が「バイオ製品およびバイオマスエネルギーの開発・普及に関する大統領令」を発令し、バイオマスエネルギーの利用率を2010年までに10％までに拡大する方針を指示し、ヨーロッパにおいても同様の方針が打ち出されている状況である。このような社会的背景も重なり、現在、グルコースから生産されるバイオエタノールはブラジル、米国をはじめとして多くの地域でガソリンと混合され、自動車用燃料として利用されている。しかしながら、食料価格高騰の問題原因ともなっており、代替原料が模索されている。木質系バイオマスはデンプンよりもはるかに豊富に存在し、また古紙や廃材、農産廃棄物など処理が問題化しているものを利用するため原料が安価であることなどから、将来の石油代替エネルギー資源として特に有望視されている。

  Japanese Society of Grassland Science, 2009, Japanese Journal of Grassland Science, 55(3) (3), 270 - 273, Japanese


• A03 In vitro and in vivo evaluation of sonocatalytic cancer therapy coupled with TiO_2 nanoparticles

  OSHIMA Shuhei, NINOMIYA Kazuaki, OGINO Chiaki, KURODA Shunichi, SHIMIZU Nobuaki

  Titanium dioxide (TiO_2) is known as sonocatalyst which generate hydroxyl radicals when the ultrasonic (US) is irradiated to TiO_2 (TiO_2/ US method). In this study, we investigated the cell damage effect and antitumor effect by the combination of ultrasonic irradiation and TiO_2 nanoparticles that is modified with preSl/S2 protein for targeting HepG2 cells. For irradiating of US to the preS1/S2-TiO_2 nanoparticles incorporated HepG2, cell injuring efficiency was evaluated by Trypan blue dye exclusion test. As a result, the cytostatic activity was acquired when irradiated at 1MHz, 0.1 W/ cm^2, 30 second, 50% duty in the presence of TiO_2 nanoparticle modified with preS1/S2 protein. This cytostatic activity was observed at 24 hour after US irradiation, and this effect was significantly enhanced as time elapsed. Also, antitumor effect by TiO_2 / U.S. method in vivo was evaluated. When ultrasonic irradiation was performed by the frequency of 3 times per week, and the irradiation conditions of 1 MHz, 1 W/cm^2, 1 min, no significant damages to the mice was ovserved. These irradiation condition revealed the antitumor effect significantly. Moreover the further research, such as the examination of the irradiation conditions of ultrasound, injection amount of TiO_2 nanoparticles and the specific binding ability to HepG2 tumor after i.v. injection, are proposed.

  The Japan Society of Sonochemistry, 2009, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 18, 5 - 6, Japanese


• 菌体触媒を用いたバイオディーゼル生産におけるイオン液体の利用

  中島 一紀, 荒井 章吾, 谷野 孝徳, 荻野 千秋, 近藤 昭彦, 福田 秀樹

  公益社団法人 化学工学会, 2009, 化学工学会 研究発表講演要旨集, 2009, 912 - 912, Japanese


• 糖代謝ネットワークを強化した酵母による混合糖からのバイオエタノール生産

  堀田 淳史, 伊藤 友教, 内田 敦子, 谷野 孝徳, 荻野 千秋, 大村 直人

  公益社団法人 化学工学会, 2009, 化学工学会 研究発表講演要旨集, 2009, 242 - 242, Japanese


• Specific Protein Delivery to Target Cells by Antibody-displaying Bionanocapsules

  Naoya Kurata, Takuya Shishido, Masaru Muraoka, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Dec. 2008, JOURNAL OF BIOCHEMISTRY, 144(6) (6), 701 - 707, English

  [Refereed]

  Scientific journal


• Construction of an Aspergillus oryzae cell-surface display system using a putative GPI-anchored protein

  Takashi Adachi, Junji Ito, Kouji Kawata, Masahiro Kaya, Hiroki Ishida, Hiroshi Sahara, Yoji Hata, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Dec. 2008, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 81(4) (4), 711 - 719, English

  [Refereed]

  Scientific journal


• Sonocatalytic facilitation of hydroxyl radical generation in the presence of TiO(2)

  Nobuaki Shimizu, Chiaki Ogino, Mahmoud Farshbaf Dadjour, Kazuaki Ninomiya, Atsushi Fujihira, Kazumichi Sakiyama

  Sep. 2008, ULTRASONICS SONOCHEMISTRY, 15(6) (6), 988 - 994, English

  [Refereed]

  Scientific journal


• Characterization of yeast cell surface displayed Aspergillus oryzae beta-glucosidase 1 high hydrolytic activity for soybean isoflavone

  Junji Ito, Hiroshi Sahara, Masahiko Kaya, Yoji Hata, Seiji Shibasaki, Koji Kawata, Saori Ishida, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Sep. 2008, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 55(1-2) (1-2), 69 - 75, English

  [Refereed]

  Scientific journal


• Bioethanol Production from Sugars by Yeast Having Sugar Uptake Ability by Expression of Sugar Transporter

  TANINO Takanori, OGINO Chiaki, KUMAGAI Norihisa, OHMURA Naoto

  Sep. 2008, Proceedings of the 10th International Chemical and Biological Engineering Conference-CHEMPOR 2008, Braga, Portugal (on CD-ROM), E20, 4pages, English

  [Refereed]

  Scientific journal


• Properties of TiO2-Polyacrylic acid (PAA) Dispersions having Molecular Recognition Ability

  KANEHIRA K, BANZAI T, OGINO Chiaki, SHIMIZU N, KUBOTA Y, SONEZAKI S

  Jun. 2008, Colloids and Surfaces B: Biointerfaces, Vol 64, pp. 10-15, English

  [Refereed]

  Scientific journal


• Yeast-based fluorescence reporter assay of G protein-coupled receptor signalling for flow cytometric screening: FAR1-disruption recovers loss of episomal plasmid caused by signalling in yeast

  Jun Ishii, Tsutomu Tanaka, Shizuka Matsumura, Kenji Tatematsu, Shun'Ichi Kuroda, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  May 2008, Journal of Biochemistry, 143(5) (5), 667 - 674, English

  [Refereed]

  Scientific journal


• Isoflavone aglycones production from isoflavone glycosides by display of beta-glucosidase from Aspergillus oryzae on yeast cell surface

  Masahiko Kaya, Junji Ito, Atsushi Kotaka, Kengo Matsumura, Hiroki Bando, Hiroshi Sahara, Chiaki Ogino, Seiji Shibasaki, Kouichi Kuroda, Mitsuyoshi Ueda, Akihiko Kondo, Yoji Hata

  May 2008, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 79(1) (1), 51 - 60, English

  [Refereed]

  Scientific journal


• Decolorization of methylene blue in aqueous suspensions of titanium peroxide

  Chiaki Ogino, Mahmoud Farshbaf Dadjour, Yasuo Iida, Nobuaki Shimizu

  May 2008, JOURNAL OF HAZARDOUS MATERIALS, 153(1-2) (1-2), 551 - 556, English

  [Refereed]

  Scientific journal


• 機能性二酸化チタン・ナノ粒子のがん治療への基礎研究

  笹井 良祐, 園家 史朗, 大島 周平, 荻野 千秋, 黒田 俊一, 清水 宣明

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 328 - 328, Japanese


• セルラーゼ発現酵母によるセルロースからのバイオエタノール生産

  柳瀬 修平, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 990 - 990, Japanese


• A03 An effective disinfection of microorganisms by sonocatalytic method with TiO_2(Oral session)

  ARAKAWA Masayuki, RAHAMAN MOHAMMAD MIZANUR, NINOMIYA Kazuhiro, OGINO Chiaki, SHIMIZU Nobuaki

  To well characterize disinfection system and to investigate the mechanisms of the cell killing by TiO_2/U.S. method, Escherichia coli (NOVABLUE strain) was used as a model and target microorganism. Our previous studies reported that ultrasonic irradiation was very effective to kill the microbial cells. Microbial cells are physically and chemically sterilized by ultrasonic irradiation. Our present study was under taken, to investigate a suitable mechanism for cells killing and to develop the sterilization effect using electrochemically under U.S. in the presence of TiO_2. We suggested that, toxic substances such as free OH radical generated by electrolysis have been shown to be responsible for the sterilization effect.

  The Japan Society of Sonochemistry, 2008, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 17, 16 - 17, Japanese


• P04 Ultrasonically induced membrane lipid peroxidation of Escherichia coli in the presence of TiO_2, towards an understanding of its killing mechanisms(Part II,Poster session)

  RAHMAN Mohammad MIZANUR, ARAKAWA Masayuki, NINOMIYA Kazuhiro, OGINO Chiaki, SHIMIZU Nobuaki

  Strong bacteriocidal activity of titanium dioxide (TiO_2) irradiated with ultrasound (U.S.) is an established phenomenon. A new non-woven TiO_2 fabric under U.S. system was used to well characterize the sonocatalytic disinfection and lipid peroxidation of Escherichia coli (E. coli) cells membrane and to investigate the possible killing mechanisms of its cell. It was found that applying ultrasonic irradiation with non-woven TiO_2 fabrics reduces the E. coli cell concentration very rapidly, which indicated very high disinfection rate at this system. Diphenyl-1-pyrenylphosphin (DPPP) was used as a non-fluorescent probe for lipid peroxidation on E. coli cells membrane. Reaction of DPPP with U.S. treated samples and organic hydroperoxides were examined by monitoring increase in fluorescence intensity of DPPP oxide (DPPP=O). The mechanism of cell killing and lipid peroxidation were further investigated by examining the effect of OH radical scavenger. The scavenger studies indicated the functional role of hydroxyl (OH) radicals in the initiation of ultrasound induced lipid peroxidation.

  The Japan Society of Sonochemistry, 2008, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 17, 48 - 49, English


• P05 Effect of frequency on OH radical formation caused by ultrasonic irradiation with TiO_2(Part I,Poster session)

  ONIWA Tatsuya, NINOMIYA Kazuaki, OGINO Chiaki, SHIMIZU Nobuaki

  Effects of TiO_2 particles addition on OH radical formation by ultrasonic irradiation were investigated through comparison of Al_2O_3 particles. Formation of cavitation bubbles is accelerated on the surface of particles because sonoluminescence intensity and white noise intensity are increased by particle addition. It is suggested that there is the close relationship between the effeciency of TiO_2 on OH radical formation and cavitation phenomena. Irradiation frequency is the very important factor for cavitation phenomena. In this reseach, the effects of frequency changes on OH radical formation by ultrasonic irradiation were investigated in the presence of TiO_2.

  The Japan Society of Sonochemistry, 2008, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 17, 50 - 51, Japanese


• P28 Evaluation of sonocatalytic cytotoxicity of cancer cells with incorporation of TiO_2 nanoparticles(Part II,Poster session)

  NINOMIYA Kazuaki, SONOKE Shiro, OSHIMA Shuhei, OGINO Chiaki, KURODA Shun'ichi, SHIMIZU Nobuaki

  Our previous study demonstrated that the titanium dioxide (TiO_2) could catalyze production of hydroxyl (OH) radicals by ultrasound (U.S.) irradiation even without any photo-excitation. The purpose of this study was to extend the applicability of our "TiO_2/U.S. system" to drug delivery systems for cancer therapy by modifying the surface of TiO_2 nanoparticles with PreS1/S2 protein for targeting HepG2 cells. To evaluate the specific binding of the preS1/S2-conjugated TiO_2 particles (preS1/S2-TiO_2), liver and colon cancer cell lines, HepG2 and WiDr were incubated with the preS1/S2-TiO_2 (0.01% (v/v)) for 6 hours and subjected to fluorescent immunostaining with anti-preS1 antibody. The fluorescence was detected only in the case of HepG2 cells, indicating specific binding and incorporation of preS1/S2-TiO_2. To evaluate the sonocatalytic cytotoxicity of HepG2 cells with incorporation of preS1/S2-TiO_2, the culture dish was irradiated with U.S. at the frequency of 1MHz and with output power of 0.3 W/m^2 for 30 sec (50% duty). Compared to the control condition without preS1/S2-TiO_2 incorporation and/or U.S. irradiation, the growth of HepG2 cells was restrained with both the preS1/S2-TiO_2 incorporation and the U.S. irradiation at initial time of culture, suggesting our "TiO_2/U.S. system" could be promising tool for cancer therapy.

  The Japan Society of Sonochemistry, 2008, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 17, 96 - 97, Japanese


• 放線菌でのタンパク質分泌生産に関わるシグナル配列の解析

  吉野 和慶, 伊藤 吉基, 宮下 徹, 伊藤 裕子, 清水 宣明, 荻野 千秋

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 380 - 380, Japanese


• 放線菌を用いたリン脂質代謝酵素の変異体解析

  伊藤 吉基, 伊藤 裕子, 宮下 徹, 吉野 和慶, 清水 宣明, 荻野 千秋

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 381 - 381, Japanese


• フローサイトメーター(FCM)を利用した酵母GPCRシグナリング解析技術

  石井 純, 田中 勉, 松村 静香, 立松 健司, 黒田 俊一, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 348 - 348, Japanese


• 二酸化チタンへの超音波照射による過酸化水素生成機構の解析

  崎山 和亨, 大庭 達哉, 荻野 千秋, 清水 宣明

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 225 - 225, Japanese


• Growth acceleration of plants and mushroom by erythritol

  Masahiko Kaya, Junji Ito, Atsushi Kotaka, Kengo Matsumura, Hiroki Bando, Hiroshi Sahara, Chiaki Ogino, Seiji Shibasaki, Kouichi Kuroda, Mitsuyoshi Ueda, Akihiko Kondo, Yoji Hata

  2008, Appl. Microbiol. Biotechnol., 79: 51-60, English

  [Refereed]


• Recognition and effective degradation of 17 beta-estradiol by anti-estradiol-antibody-immobilized TiO2 nanoparticles

  Chiaki Ogino, Koki Kanehira, Ryosuke Sasai, Shuji Sonezaki, Nobuaki Shimizu

  Oct. 2007, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 104(4) (4), 339 - 342, English

  [Refereed]

  Scientific journal


• Continuous production of phospholipase D using immobilized recombinant Streptomyces lividans

  Chiaki Ogino, Masayuki Kanemasu, Masashi Fukumoto, Takafumi Kubo, Takamichi Yoshino, Akihiko Kondo, Hideki Fukuda, Nobuaki Shimizu

  Jul. 2007, ENZYME AND MICROBIAL TECHNOLOGY, 41(1-2) (1-2), 156 - 161, English

  [Refereed]

  Scientific journal


• Remarkable enhancement in PLD activity from Streptoverticillium cinnamoneum by substituting serine residue into the GG/GS motif

  Chiaki Ogino, Hidenori Daido, Yuka Ohmura, Namiko Takada, Yoshiki Itou, Akihiko Kondo, Hideki Fukuda, Nobuaki Shimizu

  Jun. 2007, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1774(6) (6), 671 - 678, English

  [Refereed]

  Scientific journal


• Sonocatalytic degradation of methylene blue with TiO2 pellets in water

  Nobuaki Shimizu, Chiaki Ogino, Mahmoud Farshbaf Dadjour, Tomoyuki Murata

  Feb. 2007, ULTRASONICS SONOCHEMISTRY, 14(2) (2), 184 - 190, English

  [Refereed]

  Scientific journal


• S3-5 Construction of biomolecule-immobilized TiO_2 nanoparticle for applying to new cell injuring method with ultrasound irradiation(Symposium (3) Biomedical Application)

  Ogino Chiaki, Sasai Ryousuke, Sonoke Shirou, Kuroda Shunichi, Shimizu Nobuaki

  The Japan Society of Sonochemistry, 2007, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 2007, 50 - 51, English


• 組織ターゲティング機能を有する二酸化チタンナノ粒子によるがん細胞障害

  柴田 直範, 笹井 良祐, 高木 圭子, 黒田 俊一, 荻野 千秋, 清水 宣明

  公益社団法人 化学工学会, 2007, 化学工学会 研究発表講演要旨集, 2007, 391 - 391, Japanese


• P-34 Analysis of Sonodynamic Sterilization of Escherichia coli at the Surface of TiO_2(Poster Presentations)

  Rahman Mohammad Mizanur, Ogino Chiaki, Shimizu Nobuaki

  The Japan Society of Sonochemistry, 2007, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 2007, 102 - 102, English


• 放線菌の新規タンパク質発現系構築に関する研究

  久保 孝文, 吉野 和慶, 伊藤 吉基, 宮下 徹, 荻野 千秋, 清水 宣明

  公益社団法人 化学工学会, 2007, 化学工学会 研究発表講演要旨集, 2007, 353 - 353, Japanese


• Enhancement of sonocatalytic cell lysis of Escherichia coli in the presence of TiO2

  Chiaki Ogino, Mahmoud Farshbaf Dadjour, Keiko Takaki, Nobuaki Shimizu

  Nov. 2006, BIOCHEMICAL ENGINEERING JOURNAL, 32(2) (2), 100 - 105, English

  [Refereed]

  Scientific journal


• Disinfection of Legionella pneumophila by ultrasonic treatment with TiO2

  MF Dadjour, C Ogino, S Matsumura, S Nakamura, N Shimizu

  Mar. 2006, WATER RESEARCH, 40(6) (6), 1137 - 1142, English

  [Refereed]

  Scientific journal


• A1 Cytotoxic effect of ultrasonic irradiation in the presence of TiO_2 particles

  Takaki Keiko, ogino Chiaki, Shimizu Nobuaki

  Titanium dioxide (TiO_2) is known as photocatalytic material, and it generate reactive oxygen species (ROS) according to be excited by ultraviolet rays (UV) under 380nm. Those ROS generated from TiO_2 could sterilize E coli, and have cytotoxic effect on mammalian cancer cells. Previously, we reported the generation of OH radicals from TiO_2 particles excited by ultrasonic (US) irradiation, not UV. Furthermore, the TiO_2 excited by US exhibited decompose activity on E coli and chemical substances. In this study, we report the cytotoxic effect of US in the presence of TiO_2 particles in cell culture media. To estimate the cytotoxic effect, we measured lactase dehydrogenase (LDH) activity leaked from damaged cell numbers after US irradiation. Immediately after US irradiation in the presence of TiO_2 particles, the LDH activity exhibited high, indicating TiO_2 with US irradiation caused damage on cell membrane stability. Furthermore, we confirmed the cell membrane damage by flowcytometry assay with Annexin V-FITC/Propidium iodide (PI) stain method, Annexin V-negative/PI positive cells were dominant immediately after US irradiation in the presence of TiO_2 particles.

  The Japan Society of Sonochemistry, 2006, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 15, 1 - 2, Japanese


• P27 Dissolved argon facilitates the formation of OH radicals in the ultrasound/TiO_2 system(Poster Session)

  FUJIHIRA Atsushi, SAKIYAMA Kazumichi, OGINO Chiaki, SHIMIZU Nobuaki

  Generation of OH radicals by ultrasound irradiation in the presence of TiO_2 was studied. The effects of dissolved argon and xenon applied into the irradiating solutions were studied in the presence of TiO_2. Heat capacity ratio of argon and xenon is higher than that of nitrogen and oxygen. Higher heat capacity ratio of dissolved argon and xenon causes higher local temperature when cavitation bubble collapsed, and it results in the induced pyrolysis of H_2O and generation of OH radicals. Further, the effect of the photocatalysis of TiO_2 may be more intensified by high temperature and sonoluminescence.

  The Japan Society of Sonochemistry, 2006, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 15, 99 - 100, Japanese


• P21 Analysis of H_2O_2 formation induced by ultrasonic irradiation with TiO_2(Poster Session)

  SAKIYAMA Kazumichi, FUJIHIRA Atushi, OGINO Chiaki, TAKAKI Keiko, SHIMIZU Nobuaki

  The formation of H_2O_2 was studied with ultrasound irradiation in the presence of TiO_2 or Al_2O_3 particles. The effect of varying the temperature of cavitation bubbles in aqueous solutions of two rare gases (Ar, Xe or mixture of Xe and Ne (Xe:Ne=3:7)) on the formation of H_2O_2 were estimated. The very high temperatures (several thousand K) of collapsing gas bubbles lead to the thermal dissociation of water molecules into OH radicals, and the reaction of two OH radicals causes the formation of H_2O_2 consequently. After 60 min sonication with 25〜36kHz ultrasound the highest concentration of H_2O_2 (612μM) was observed when Xe was saturated in the solution. While the increased concentration of H_2O_2 after 60 min sonication was lower (135μM) in the solution saturated with Ar or mixture of Xe and Ne. The difference in the increased concentration of H_2O_2 was not significant between Ar and mixture of Xe and Ne. These results suggest that the threshold for the formation of cavitation bubbles seems to be the lowest in the solution saturated with Xe and to be the important factor for the formation of H_2O_2 during the sonication in the presence of TiO_2 or Al_2O_3, respectively.

  The Japan Society of Sonochemistry, 2006, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 15, 87 - 88, Japanese


• P29 Sonodynamic sterilization of microbial cells at the surface of TiO_2(Poster Session)

  Rahman Mohammad Mizanur, Ogino Chiaki, Shimizu Nobuaki

  To well characterize disinfection system and to investigate the mechanism of cell killing by TiO_2/US method, Escherichia coli (E. coli) was used as a model and target microorganism. At present, our research is going on developing disinfection system by using ultrasonic reactor in the presence of nonwoven TiO_2 fabric. It was found that the decrease in cell concentration was almost insignificant without ultrasonic irradiation although TiO_2 filter was present, and vice versa. In contrast, applying ultrasonic irradiation with TiO_2 filter reduces the E. coli cell concentration very rapidly which indicated very high disinfection power at this condition or system. However, the effect of new ultrasonic reactors on the disinfection rate, the characterization of affecting parameters of the ultrasonic reactors, as well as possible germicidal mechanism of disinfection remain to be studied. Given an opportunity to conduct these research will uncover above issues and will be benefited our scientific career to develop, in addition to enrich the bio-industrial field as well.

  The Japan Society of Sonochemistry, 2006, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 15, 103 - 104, English


• 8OH-dGを認識するDNAアプタマーに関する基礎研究

  宮地 祐介, 荻野 千秋, 清水 宣明

  公益社団法人 化学工学会, 2006, 化学工学会 研究発表講演要旨集, 2006, 997 - 997, Japanese


• 二酸化チタン・超音波法によるOHラジカルの生成と溶存アルゴンの促進効果

  藤平 敦司, 崎山 和享, 荻野 千秋, 清水 宣明

  公益社団法人 化学工学会, 2006, 化学工学会 研究発表講演要旨集, 2006, 783 - 783, Japanese


• Kinetics of disinfection of Escherichia coli by catalytic ultrasonic irradiation with TiO2

  MF Dadjour, C Ogino, S Matsumura, N Shimizu

  Oct. 2005, BIOCHEMICAL ENGINEERING JOURNAL, 25(3) (3), 243 - 248, English

  [Refereed]

  Scientific journal


• A5 Augmentation of ultrasonic disinfection power with TiO_2 photocatalyst

  Dadjour Mahmoud Farshbaf, Ogino Chiaki, Matsumura Susumu, Shimizu Nobuaki

  The Japan Society of Sonochemistry, 2005, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 14, 13 - 14, English


• P8 Dissolved argon facilitates the formation of OH radicals in the ultrasound/TiO_2 system

  Fujihira Atsushi, Ogino Chiaki, Dadjour Mahmoud Farshbaf, Shimizu Nobuaki

  Generation of OH radicals by ultrasonic irradiation in the presence of TiO_2 was studied. OH radicals are oxidizing agents and effective for the degradation of hazardous organic compounds. However, the mechanisms of OH radicals formation have not been elucidated yet. In this work, the effects of cavitation induced by ultrasonic irradiation in water was considered and investigated. As the lifetime of OH radical is very short and can be hardly analyzed directly, then sodium salicylate was used as OH radicals trapping agent. The addition reaction of OH radicals and salicylate results in the formation of dihydroxy benzoic acid(DHBA). The generation of DHBA by ultrasonic irradiation in the presence of TiO_2 as an indicator of OH radicals was investigated in complete darkness. The effects of particles (TiO_2 and A1_2O_3) on the generation of DHBA were examined(Fig.2). While no generation of DHBA was detected in the solutions without particles, higher DHBA concentration was obtained in the presence of TiO_2 than that of Al_2O_3. This enhancement in the formation of OH radicals may be due to the pyrolysis of H_2O and the photocatalysis of TiO_2 with sonoluminessence. Sonoluminessence may be due to cavitation, and the exsitence of particles couse more cavitation whuch may increase sonoluminessence effect. [graph] Also, the effects of dissolved argon applied into the irradiating solutions were studied in the presence of TiO_2 and Al_2O_2 (Fig.5, 6). Higher amount of DHBA was obtained in the solutions in the presence of TiO_2. Thermal capacity of argon is higher than that of nitrogen and oxygen. Higher thermal capacity of dissolved argon causes higher local temperature during cavitation, and it results in the induced pyrolysis of H_2O and generation of OH radicals. Moreover, the effect of the photocatalysis of TiO_2 may be more intensified in the presence of dissolved argon. [graph] [graph] Consequently, the advantage of TiO_2 in the ultrasonic system for the formation of OH radicals was shown in this research.

  The Japan Society of Sonochemistry, 2005, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 14, 91 - 93, Japanese


• 放線菌を宿主とするタンパク質発現系の構築

  福本 将士, 荻野 千秋, 清水 宣明

  公益社団法人 化学工学会, 2005, 化学工学会 研究発表講演要旨集, 2005, 287 - 287, Japanese


• リン脂質代謝酵素の蓋構造変化における活性相関に関する研究

  泉 麻里子, 荻野 千秋, 清水 宣明

  公益社団法人 化学工学会, 2005, 化学工学会 研究発表講演要旨集, 2005, 280 - 280, Japanese


• Improvement of ultrasonic disinfection power using TiO2 photocatalyst

  MF Dadjour, C Ogino, S Matsumura, S Nakamura, N Shimizu

  2005, 4th International Symposium on Therapeutic Ultrasound, 754, 275 - 277, English

  [Refereed]

  International conference proceedings


• Over-expression system of phospholipase D from actinomycete by Streptomyces lividans

  OGINO Chiaki, KANEMASU M, HAYASHI Y, KURODA S, KONDO Akihiko, SHMIZU N, TANIZAWA K, FUKUDA Hideki

  Jun. 2004, Applied Microbiology and Biotechnology, Vol 64, pp. 823-828, English

  [Refereed]

  Scientific journal


• Enzymatic kinetics for lipase-catalyzed hydrolysis of water-insoluble substrate in AOT reverse micelles, involving native and activated lipases

  NMR Talukder, MM Zaman, Y Hayashi, JC Wu, T Kawanishi, C Ogino, N Shimizu

  Mar. 2004, JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 79(3) (3), 273 - 276, English

  [Refereed]

  Scientific journal


• Investigation of the Catalytic Function of Phospholipase D by Site Directed Mutagenesis

  Ogino Chiaki, Izumi Mariko, Ohishi Takako, Fukumoto Masashi, Shimizu Nobuaki

  A phospholipase D (PLD) expression system was constructed from Streptoverticillium cinnamoneum using Escherichia coli as a host. From a sequence alignment analysis of PLD superfamily, a conserved sequence, a GG/S motif, was found, next to the putative active center in the PLD superfamily. Based on the hypothesis that a serine residue in GG/S motif is involved in the catalytic function, a serine residue substituted mutant library (sixteen PLD mutants) was constructed. An analysis of the activities of these mutants showed that two serine residue substituted mutants (G215S and G216S) in the GG motif exhibited high conversion activity. However, the substrate specificity of these mutants differed depending on the donor molecules. When a serine amino acid was used as a donor substrate, G216S showed a high transphosphatidylation activity. For other donors, G215S showed a higher catalytic activity than G216S. These results support the hypothesis that the serine residue in the GG/S motif controls substrate specificity and enhances the turnover of catalytic function.

  The Society of Chemical Engineers, Japan, 2004, Asian Pacific Confederation of Chemical Engineering congress program and abstracts, 2004, 810 - 810, English


• Cell Disinfection by Ultrasonic Irradiation with TiO₂ Photocatalyst

  Farshbaf Mahmoud Dadjour, Ogino Chiaki, Matsumura Susumu, Nakamura Shinichi, Shimizu Nobuaki

  A disinfecting method has been considered by ultrasonic irradiation of water in the presence of TiO₂. Titanium dioxide in the rutile-granule form was used as a catalyst in order to generate hydroxyl radicals for cell killing. Escherichia coli and Legionella pneumophila were used for kinetically investigation of cell destruction process. Significant decrease in the concentration of viable cells was observed during irradiation in the presence of TiO₂. About 98% reduction in the concentration of viable cells could be obtained in the presence of 1.0 g/ml TiO₂ during 30 min of irradiation, while only 13% reduction was observed in the ordinary ultrasonic irradiation system. The rate of cell killing was higher in the presence of TiO₂ comparing to Al₂O₃. The rate of disinfection was proportional to the amount of TiO₂ used in the irradiated samples. There was no significant effect of cell concentration on the cell-killing rate in the range of 10³ to 10⁷ CFU/ml. Mechanism of cell killing was further investigated by examining the effect of hydroxyl radical (*OH) scavengers. Ascorbic acid, histidine and glutathione were used in the irradiating samples as radical scavengers. The rate of disinfection was decreased in the samples containing these radical scavengers. Also, a continuous irradiating system was devised and applied for high throughput water disinfection process. Contaminated water containing E. coli was disinfected successfully within two hours of irradiation in the continuous system.

  The Society of Chemical Engineers, Japan, 2004, Asian Pacific Confederation of Chemical Engineering congress program and abstracts, 2004, 610 - 610, English


• SII-4 Enhancement of ultrasonic disinfection of Legionella with TiO_2 photocatalyst(Special session II (Environmental protection))

  Matsumura S., Farshbaf D.M., Yoshikawa F., Ogino C., Shimizu N.

  Introduction Titanium dioxide in the rutile-pellet form was used as a catalyst in the ultrasonic irradiation system to generate hydroxyl radicals in order to disinfect the water. Cultivated culture of Legionella was used for the kinetically investigation of cell destruction and its mechanism. The formation of active species such as hydroxyl radical, hydrogen peroxide, superoxide and etc can be promoted during ultrasonic irradiation in the presence of TiO_2. It may be due to the concentrated energy of the exploded bubbles on the surfaces of titanium granules caused during acoustic cavitations. These reactive oxygen species (ROS) may disrupt or damage various cell or viral functions and structures. Improvement of this novel disinfection method and studying the mechanism of this system were the purposes of this investigation. Materials and methods -Preparation of Cultures Legionella pneumophila(GIFU 9888) was used in this research as the model microorganisms. Legionella were incubated on BCYE plate and transferred to BYE medium. The inoculated culture was incubated in a shaker incubator at 110 rpm for 48 hrs at 37℃. Then, the culture broth was diluted to a suitable concentration. Ten ml of the diluted broth was transferred to the bottles containing TiO_2 pellets at different concentrations. -Ultrasonic irradiation Bottles were irradiated at 36 KHz, 300 W and samples were driven at different time courses. The temperature was kept constant at 20℃ during irradiation. The irradiated samples were diluted to suitable concentrations and plated on BCYE plates. The plates were incubated for 72 hrs at 37℃. The number of colonies on the plates was counted after incubation. Results and Discussion Ultrasonic irradiation of diluted culture for 15 minutes showed 92% reduction in the number of viable cell concentration at the presence of TiO_2. The rate of disinfection was compared in the presence of TiO_2 and Al_2O_3. TiO_2 induced higher rate of cell killing compared with Al_2O_3. Also, investigating the effect of TiO_2 concentration indicated that the rate of disinfection was proportional to the concentration of TiO_2. The mechanism of cell killing was further investigated by examining the effects of OH radical scavengers such as ascorbic acid and glutathione. Lower rates of disinfection were observed in the samples containing these radical scavengers, thus indicating the primary role of OH radicals in this process.

  The Japan Society of Sonochemistry, 2004, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 13, 38 - 40, Japanese


• P-7 Generation of OH radicals under ultrasonic irradiation with TiO_2(Poster presentation)

  Funakoshi M., Farshbaf D., Yoshikawa F., Ogino C., Shimizu N.

  Irradiation of TiO_2 particles under ultraviolet light has been known to be a source of OH radicals in the disinfection or oxidizing systems. However, a new system was devised in this study for production of OH radicals by replacing UV with ultrasonic system (US). Formation of OH radicals was investigated in a TiO_2/US system to study the mechanism of formation of OH radicals as well as enhancement of cavitation effect in the ultrasonic system. Formation of OH radicals in the irradiating solution was studied by the reaction of OH radicals with salicylate. 2,3-dihydroxybenzoicacid (2,3-DHBA) and 2,5-dihydroxybenzoicacid (2,5-DHBA) can be formed during this reaction and they can be measured by HPLC with an electro chemical detector (HPLC-ECD; Eicom). Formation of DHBAs could be detected during the irradiation of salicylate solution under ultrasonic system and in the presence of TiO_2. It was seen that the concentration of DHBAs was increased within 20 minutes irradiation and then decreased in spite of high concentration of salicylate. It was likely due to the oxidation and degradation of DHBA in the ultrasonic system. Therefore, for investigating the oxidation of DHBA in the presence of salicylate, more salicylate was added after 20 minutes reaction to the irradiating mixture consecutively. The concentration of DHBA was increased for three times by the addition of salicylate concentration in the solution. Enhancement of cavitation energy was also investigated by the addition of various gases (Ar, O2, N2) into irradiation solution. The ratio of the specific heats at constant pressure and constant volume (Cp/Cv) of the gas and vapor inside the cavity is known to be one of the most crucial parameters that determine the final temperature inside cavitation bubbles. Monoatomic gases, such as argon has a high value of Cp/Cv, while diatomic gases, such as N_2, O_2 have a lower values. Therefore, addition of argon to the irradiating solution induced higher concentration of OH radicals due to higher cavitation energy. Our results indicated that the addition of O_2 and N_2 gases at the same conditions resulted lower oxidation power. Finally, the competitive oxidation of salicylate and DHBAs in the irradiating solutions was more investigated in this research to develop a more descriptive mechanism of generation of hydroxyl radicals.

  The Japan Society of Sonochemistry, 2004, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 13, 94 - 96, Japanese


• Over-expression system for secretory phospholipase D by Streptomyces lividans

  Ogino, C., Kanemasu, M., Hayashi, Y., Kondo, A., Shimizu, N., Tokuyama, S., Tahara, Y., Kuroda, S., Tanizawa, K., Fukuda, H.

  2004, Applied Microbiology and Biotechnology, 64(6) (6), English

  [Refereed]

  Scientific journal


• Effect of medium compositions on biosensing of benzene derivatives using recombinant Escherichia coli

  S Ikeno, C Ogino, T Ito, Y Sugino, N Shimizu

  Dec. 2003, BIOCHEMICAL ENGINEERING JOURNAL, 16(3) (3), 273 - 278, English

  [Refereed]

  Scientific journal


• Phospholipase D from Streptoverticillium cinnamoneum: protein engineering and application for phospholipid production

  C Ogino, S Kuroda, S Tokuyama, A Kondo, N Shimizu, K Tanizawa, H Fukuda

  Sep. 2003, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 23(2-6) (2-6), 107 - 115, English

  [Refereed]

  Scientific journal


• Detection of benzene derivatives by recombinant E-coli with Ps promoter and GFP as a reporter protein

  S Ikeno, C Ogino, T Ito, N Shimizu

  Sep. 2003, BIOCHEMICAL ENGINEERING JOURNAL, 15(3) (3), 193 - 197, English

  [Refereed]

  Scientific journal


• Improvement in enzyme activity and stability by addition of low molecular weight polyethylene glycol to sodium bis(2-ethyl-L-hexyl)sulfosuccinate/isooctane reverse micellar system

  MMR Talukder, T Takeyama, Y Hayashi, JC Wu, T Kawanishi, N Shimizu, C Ogino

  Aug. 2003, APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 110(2) (2), 101 - 112, English

  [Refereed]

  Scientific journal


• 8 Sonocatalytic disinfection of Escherichia coli with TiO_2 photocatalyst(Medical and Biochemical Application)

  Farshbaf D.M., Shimizu N., Matsumura S., Ogino C.

  The Japan Society of Sonochemistry, 2003, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 12, 30 - 32, Japanese


• Structural investigation of water trapped in AOT/isooctane reverse micelles containing PEG by Fourier transform infrared spectroscopy

  T Takeyama, Y Hayashi, M Talukder, N Shimizu, T Kawanishi, C Ogino

  Jan. 2003, KAGAKU KOGAKU RONBUNSHU, 29(1) (1), 124 - 130, Japanese

  [Refereed]

  Scientific journal


• Improvement of transphosphatidylation reaction model of phospholipase D from Streptoverticillium cinnamoneum

  C Ogino, Y Yasuda, A Kondo, N Shimizu, H Fukuda

  Mar. 2002, BIOCHEMICAL ENGINEERING JOURNAL, 10(2) (2), 115 - 121, English

  [Refereed]

  Scientific journal


• 9 Significant Increase in Hydroxyl Radicals by Irradiation of Ultrasound on Titanium Dioxide in Aqueous Solution and its Application for Decomposition of Hazardous Chemical Substances

  Shimizu Nobuaki, Ogino Chiaki, Hayashi Yoshishige, Miyoshi Norio

  Heterogeneous photocatalysis over TiO_2 or ultrasonic irradiation have been reported to be a promising application for decomposition of the hazardous chemical substances. We observed the generation of ・OH in the aqueous solution during the irradiation of ultrasound on TiO_2. The purpose of this study was to verify the generation of ・OH by indicating (1) decomposition of methylene blue, (2) nucleophilic addition of ・OH to salicylic acid, which produce dihydroxybenzoic acid. The decomposition of bisphenol A (BPA) and pentachlorophenol (PCP) by the irradiation of ultrasound in presence of TiO_2 were further studied. The results are summarized as follows. Decomposition of methylene blue Experimentally it was observed that although the Al_2O_3 had no effect on the decomposition of methylene blue by ultrasonic irradiation, it was decomposed slightly by the presence of TiO_2. In addition H_2O_2 accelerated the decomposition of methylene blue for the TiO_2 containing system. But the addition of H_2O_2 had no effect on the methylene blue decomposition when the system containing Al_2O_3 only. The effect of radical scavengers on the decomposition of methylene blue was also investigated for the system with TiO_2. It was found that the scavengers such as methanol, DMSO, mannitol reduced the decomposition. Among them DMSO was the most significant. Effect of TiO_2 on the nucleophilic addition of ・OH to salicylic acid Ultrasonification of 10 μM salicylic acid (2 ml) was carried out in absence and presence of 0.4 g TiO_2 for exactly 10 minutes, and produced dihydroxybenzoic acid (DHBA) was measured by HPLC/ECD. In presence of TiO_2, significant amount of DHBA (34 pg/20μl) was produced, but almost no DHBA (2.2 pg/20μl) was found to produce without TiO_2. The effect of scavengers on the generation of DHBA was the same as it was observed in the case of methylene blue decomposition. This result inferred that the ultrasonic irradiation generated the ・OH, which is responsible for the hydroxylation of salicylic acid. Decomposition of BPA and PCP Ultrasonification of BPA and PCP, a representative endocrine disruptors, were carried out in the presence of TiO_2 (0.4 g) and the change in the concentration of BPA and PCP were measured by HPLC/ECD. The initial concentration of BPA (1 ppm) was gradually decreased with the irradiation time and was totally decomposed by 15 minute irradiation of ultrasound. The similar results were observed for PCP. It was concluded that the irradiation of ultrasound on TiO_2 in aqueous solution significantly generate hydroxyl radicals which is responsible for the decomposition of the endocrine disruptors such as BPA and PCP.

  The Japan Society of Sonochemistry, 2002, Proceedings of the Annual Meeting of the Japan Society of Sonochemistry, 11, 91 - 93, Japanese


• Fractal analysis of Daphnia motion for acute toxicity bioassay

  Nobuaki Shimizu, Chiaki Ogino, Takuya Kawanishi, Yoshishige Hayashi

  2002, Environmental Toxicology, 17(5) (5), 441 - 448, English

  [Refereed]

  Scientific journal


• Identification of novel membrane-bound phospholipase D from Streptoverticillium cinnamoneum, possessing only hydrolytic activity

  C Ogino, Y Negi, H Daido, M Kanemasu, A Kondo, S Kuroda, K Tanizawa, N Shimizu, H Fukuda

  Jan. 2001, BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1530(1) (1), 23 - 31, English

  [Refereed]

  Scientific journal


• The 5-HT1A receptor agonist, 8-OH-DPAT, attenuates stress-induced anorexia in conjunction with the suppression of hypothalamic serotonin release in rats

  N Shimizu, T Hori, C Ogino, T Kawanishi, Y Hayashi

  Dec. 2000, BRAIN RESEARCH, 887(1) (1), 178 - 182, English

  [Refereed]

  Scientific journal


• Purification, characterization, and sequence determination of phospholipase D secreted by Streptoverticillium cinnamoneum

  C Ogino, Y Negi, T Matsumiya, K Nakaoka, A Kondo, S Kuroda, S Tokuyama, U Kikkawa, T Yamane, H Fukuda

  Feb. 1999, JOURNAL OF BIOCHEMISTRY, 125(2) (2), 263 - 269, English

  [Refereed]

  Scientific journal


• Reconstitution of GTP-γ-S-dependent phospholipase D activity with ARF, RhoA, and a soluble 36-kDa protein

  Kiyoshi Shimooku, Toshihiro Akisue, Hitoshi Jinnai, Tomohiro Hitomi, Chiaki Ogino, Kimihisa Yoshida, Shun-Ichi Nakamura, Yasutomi Nishizuka

  Elsevier B.V., Jun. 1996, FEBS Letters, 387(2-3) (2-3), 141 - 144, English

  [Refereed]

  Scientific journal


• Mammalian phospholipase D: Phosphatidylethanolamine as an essential component

  Shun-Ichi Nakamura, Yoshimoto Kiyohara, Hitoshi Jinnai, Tomohiro Hitomi, Chiaki Ogino, Kimihisa Yoshida, Yasutomi Nishizuka

  Apr. 1996, Proceedings of the National Academy of Sciences of the United States of America, 93(9) (9), 4300 - 4304, English

  [Refereed]

  Scientific journal


• Mammalian phospholipase D: Activation by ammonium sulfate and nucleotides

  Shun-Ichi Nakamura, Kiyoshi Shimooku, Toshihiro Akisue, Hitoshi Jinnai, Tomohiro Hitomi, Yoshimoto Kiyohara, Chiaki Ogino, Kimihisa Yoshida, Yasutomi Nishizuka

  Dec. 1995, Proceedings of the National Academy of Sciences of the United States of America, 92(26) (26), 12319 - 12322, English

  [Refereed]

  Scientific journal

• Valorization of Lignin and Its Derivatives Using Yeast

  Filemon Jalu Nusantara Putra, Prihardi Kahar, Akihiko Kondo, Chiaki Ogino

  Oct. 2022, PROCESSES, 10(10) (10), 2004 - 2004, English

  [Refereed]

  Link to Kobe Univ. Repository (Kernel)


• バイオエコノミーの展望

  近藤昭彦, 荻野千秋

  Apr. 2022, 化学工学 [特集]「生物機能を利用したモノづくり」に貢献するプロセス強化, 86(4) (4), 151 - 152, Japanese

  Introduction scientific journal


• Comparative analysis of metabolic strategies among various yeast species

  清家泰介, KAHAR Prihardi, 荻野千秋, 松田史生

  2022, 日本生物工学会大会講演要旨集, 74th


• パーム油産業廃棄物POMEを原料とする微生物による酵素・化学品への変換

  荻野千秋, RACHMADORA Nova, KAHAR Prihardi, 近藤昭彦

  2021, 化学工学会年会研究発表講演要旨集(CD-ROM), 86th


• Breeding of organic acid assimilation yeast for bio-refinery

  壷井ひかり, KAHAR Prihardi, 近藤昭彦, 荻野千秋

  2021, 日本生物工学会大会講演要旨集, 73rd


• Polyhydroxyalkanoic acid production by an oleaginous yeast strain Lipomyces starkeyi

  中村優里, 壷井ひかり, KAHAR Prihardi, 近藤昭彦, 荻野千秋

  2021, 日本生物工学会大会講演要旨集, 73rd


• ¹³C-metabolic fluxes analysis of Yarrowia lipolytica in growth and stationary phases

  松尾有紗, 岡橋伸幸, 清家泰介, 荻野千秋, 松田史生

  2021, 日本生物工学会大会講演要旨集, 73rd


• Application of ¹³C-Metabolic flux analysis to the oleaginous yeast Yarrowia lipolytica

  松尾有紗, 岡橋伸幸, 清家泰介, 荻野千秋, 松田史生

  2021, 質量分析総合討論会講演要旨集, 69th


• 好冷性海洋細菌が産生するβ-1,3-キシラナーゼの機能解析

  谷口瑞季, 工藤基徳, 荻野千秋, 近藤昭彦, 田丸浩, 青木恭彦, 岡崎文美

  2020, 日本農芸化学会中部支部例会講演要旨集(Web), 187th


• 油脂酵母Lipomyces starkeyiの遺伝子的改変による長鎖長脂肪酸の生産

  宮本捺央, LASTINOV Amza Rezky, KAHAR Prihardi, 荻野千秋, 近藤昭彦

  2019, 日本生物工学会大会講演要旨集, 71st


• 好冷性海洋細菌Psychroflexus torquis由来β-1,3-キシラナーゼの酵素化学的諸性質

  谷口瑞季, 工藤基徳, 荻野千秋, 近藤昭彦, 田丸浩, 青木恭彦, 岡崎文美

  2019, 日本生物工学会大会講演要旨集, 71st


• 機械学習を用いた酵素のEC number予測

  渡邉直暉, 村田昌浩, 荻野千秋, 近藤昭彦, 荒木通啓, 荒木通啓

  07 Aug. 2018, 日本生物工学会大会講演要旨集, 70th, 191, Japanese


• 過酸化チタンナノ粒子の併用による放射線増感治療法の開発

  西村 勇哉, 森田 健太, 荻野 千秋, 犬伏 祥子, 佐々木 良平, 近藤 昭彦

  日本DDS学会, May 2018, 日本DDS学会学術集会プログラム予稿集, 34回, 204 - 204, Japanese


• バイオマスのイオン液体前処理と酵素糖化により得られる残渣リグニンのアルカリ酸化分解特性

  江口真央, 仁宮一章, 落合芋葉子, 黒田浩介, 柘植陽太, 荻野千秋, 當摩哲也, 高橋憲司

  05 Mar. 2018, 日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 68th, ROMBUNNO.Z14‐02‐0930, Japanese


• デンプン合成制御因子CRCTが稲わらを用いたバイオエタノール生産に及ぼす効果

  森田隆太郎, 森田隆太郎, 寺村浩, 荻野千秋, 近藤昭彦, 深山浩

  2017, 日本作物学会講演会要旨集, 243rd


• 疎水性アルコールを用いた植物バイオマスの効率的な成分分離法の開発

  寺村浩, 佐々木建吾, 白井智量, 川口秀夫, 荻野千秋, 菊地淳, 佐塚隆志, 近藤昭彦, 近藤昭彦

  14 Oct. 2016, リグニン討論会講演集, 61st, 180‐181, Japanese


• 凝集性酵母における新規な阻害剤耐性機構の解明

  糸見明穂, 紀平和枝, KAHAR Prihardi, 荻野千秋, 近藤昭彦

  25 Aug. 2016, 日本生物工学会大会講演要旨集, 68th, 316, Japanese


• Yeast breeding for fuels and chemicals production from Indonesia Culture Collection

  KAHAR Prihardi, OTSUKA Hiromi, KIHARA Chie, ITOMI Akiho, LEE Jaemin, THONTOWI Ahmad, OCTAVIANA Senlio, JALU Filemon, KANTI Atit, YOPI, OGINO Chiaki, PRASETYA Bambang, KONDO Akihiko

  13 Mar. 2016, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N316, English


• Economic Value of Current Main Use of Oil Palm Empty Fruit Bunches (EFB) and Its Potential Use for Biofuel

  SIMAMORA Manaek, OGINO Chiaki, SUNARYA Yopi, KAHAR Prihardi, PRASETYA Bambang, PRASETYA Bambang, SUBIYANTO Bambang, YAMAN Aris, MAULANA Syafrizal, MALUDIN Syafrizal, MUNAWAR Sasa Sofyan

  13 Mar. 2016, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N321, English


• Biorefinery research in Kobe and Indonesia

  OGINO Chiaki, LEE JaeMin, KAHAR Prihardi, KONDO Akihiko

  13 Mar. 2016, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N302, English


• Biorefinery as strategic approach for supporting of utilization of tropical lignocellulosic biomas

  PRASETYA Bambang, SUNARYA Yopi, HERMIATI Euis, THONTOWI Ahmad, HANAFI, SIMAMORA Manaek, OGINO Chiaki, KAHAR Prihardi

  13 Mar. 2016, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N313, English


• マンナンバイオマスからのエタノール生産:β‐マンナナーゼとβ‐マンノシダーゼを細胞表層に提示した出芽酵母の開発

  石井純, 岡崎文美, DJOHAN Apridah Cameliawati, 原清敬, 浅井菜々実, ANDRIANI Ade, 寺村浩, KAHAR Prihardi, YOPI, PRASETYA Bambang, 荻野千秋, 近藤昭彦

  05 Mar. 2016, 日本農芸化学会大会講演要旨集(Web), 2016, 4A022 (WEB ONLY), Japanese


• アルコールの添加が希硫酸前処理の効率に与える影響の解析

  寺村浩, 佐々木建吾, 白井智量, 川口秀夫, 荻野千秋, 菊地淳, 佐塚隆志, 近藤昭彦

  05 Mar. 2016, 日本農芸化学会大会講演要旨集(Web), 2016, 3A030 (WEB ONLY), Japanese


• 紙パルプの同時糖化発酵によるカフェ酸の微生物生産

  川口秀夫, 勝山陽平, DU Danyao, 鶴田祥子, 南博道, 荻野千秋, 大西康夫, 近藤昭彦

  2016, 日本農芸化学会大会講演要旨集(Web), 2016


• Titanium peroxide nanoparticles enhance antitumor efficacy through reactive oxygen species in pancreatic cancer radiation therapy

  Masao Nakayama, Ryohei Sasaki, Toru Mukohara, Chiaki Ogino, Kenta Morita, Mitsuo Umetsu, Satoshi Ohara, Kazuyoshi Sato, Chiya Numako, Seiichi Takami, Akihiko Kondo

  Aug. 2015, CANCER RESEARCH, 75, English

  Summary international conference


• 2次元NMRを用いた希硫酸前処理後固体画分の組成解析

  TERAMURA HIROSHI, SASAKI KENGO, AIKAWA SHIMPEI, MATSUDA FUMIO, OKAMOTO MAMI, SHIRAI TOMOKAZU, KAWAGUCHI HIDEO, OGINO CHIAKI, YAMASAKI MASANORI, KIKUCHI JUN, KONDO AKIHIKO

  09 Mar. 2015, 日本植物生理学会年会要旨集, 56th, 284, Japanese


• 実バイオマスを微生物変換するための新しい酵母プラットフォームの探索

  KAHAR Prihardi, LEE Jaemin, 荻野千秋, 近藤昭彦

  05 Mar. 2015, 日本農芸化学会大会講演要旨集(Web), 2015, 3B33A01 (WEB ONLY), Japanese


• バイオリファイナリー利用に向けた稲わらの多様性

  合田喬, 寺村浩, 末廣美紀, 高山隆一, 最相大輔, 山本洋, 金丸研吾, 川口秀夫, 荻野千秋, 近藤昭彦, 山崎将紀

  2015, 育種学研究, 17


• 1P-025 Improvement of astaxanthin production using metabolically engineered Xanthophyllomyces dendrorhous

  Yamamoto Keisuke, Hara Kiyotaka, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 95 - 95, Japanese


• 3P-198 Characterics studies of Streptoverticillium cinnamoneum phospholipase D promoter for gene expression

  Kashiwagi Norimasa, Nishioka Masato, Matsumoto Hana, Hirose Shuichi, Sota Masahiro, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 320 - 320, Japanese


• 2P-173 Ionic liquid pretreatment and in situ enzymatic saccharification of bagasse using biocompatible cholinium ionic liquid

  Kohori Asami, Tatsumi Mai, Endo Takatsugu, Noguchi Mana, Kuroda Kosuke, Kakuchi Ryohei, Ninomiya Kazuaki, Ogino Chiaki, Takahashi Kenji

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 218 - 218, Japanese


• 2P-170 Fran monomer production from lignocellulosic biomass pretreated and fractionated using acidic ionic liquid

  YAMAOKA Kotaro, KITA Kanae, NOGUCHI Mana, KURODA Kosuke, ENDO Takatsugu, KAKUCHI Ryohei, NINOMIYA Kazuaki, OGINO Chiaki, TAKAHASHI Kenji

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 217 - 217, Japanese


• 2P-171 High solid saccharification and subsequent fermentation from the biomass pretreated with minimum amount of ionic liquid

  Shizuta Yusei, Kohori Asami, Endo Takatsugu, Noguchi Mana, Kuroda Kosuke, Kakuchi Ryohei, Ninomiya Kazuaki, Ogino Chiaki, Takahashi Kenji

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 217 - 217, Japanese


• 2P-172 Lignomonomer production from residue obtained by ionic liquid pretreatment and enzymatic saccharification of lignocellulosic biomass

  Ochiai Kobako, Abe Megumi, Noguchi Mana, Kuroda Kosuke, Endo Takatsugu, Kakuchi Ryohei, Ninomiya Kazuaki, Ogino Chiaki, Takahashi Kenji

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 217 - 217, Japanese


• 2P-097 Metabolic engineering of L-Lactate producing Aspergillus oryzae.

  Sasakura Naoya, Wakai Satoshi, Asai Nanami, Ogino Chiaki, Tsutsumi Hiroko, Hata Youji, Kondo Akihiko

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 199 - 199, Japanese


• 過酸化チタンナノ粒子による放射線増感の検討

  中山 雅央, 佐々木 良平, 西村 英輝, 吉田 賢史, 宮脇 大輔, 赤坂 浩亮, 椋本 成俊, 村岡 修, 原田 文, ノル・シャズリナ, 上原 和之, 田中 勉, 荻野 千秋, 近藤 昭彦

  (公社)日本医学放射線学会, Feb. 2014, Japanese Journal of Radiology, 32(Suppl.) (Suppl.), 37 - 37, Japanese


• 3P-045 Effects of hydrolysate of lignocellulosic biomass on phenyllactate fermentation by recombinant Escherichia coli

  Kawaguchi Hideo, Teramura Hiroshi, Niimi-Nakamura Satoko, Ogino Chiaki, Hara Kiyotaka, Hasunuma Tomohisa, Oinuma Ken-Ichi, Takaya Naoki, Hirano Ko, Sazuka Takashi, Kitano Hidemi, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 206 - 206, Japanese


• 3P-226 A display of pH-sensitive fusogenic GALA peptide facilitates endosomal escape for a Bio-nanocapsule

  Nishimura Yuya, Ezawa Ryosuke, Ishii Jun, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 251 - 251, Japanese


• 1P-219 Membrane process increased ethanol production from pretreated rice straw.

  Sasaki Kengo, Tsuge Yota, Sasaki Daisuke, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 72 - 72, Japanese


• 1P-044 Simultaneous saccharification and fermentation of D-lactic acid from starch by Aspergillus oryzae

  Wakai Satoshi, Asai-Nakashima Nanami, Ogino Chiaki, Tsutsumi Hiroko, Hata Yoji, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 28 - 28, Japanese


• 1P-112 Production of p-aminobenzoic acid by Streptomyces lividans expressing pabAB

  Okai Naoko, Satou Yoshihiro, Ohno Maya, Takeshima Yasunobu, Masuda Takaya, Miyamoto Masanori, Toida Kouzou, Ogino Chiaki, Kondou Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 45 - 45, Japanese


• Direct isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display system

  相馬 悠希, 猪熊 健太郎, 田中 勉, 荻野 千秋, 近藤 昭彦, 岡本 正宏, 花井 泰三

  日本生物工学会, 2014, 生物工学会誌 : seibutsu-kogaku kaishi, 92(2) (2), 71 - 71, Japanese


• 超音波触媒法による胆管ステント再閉塞予防の検討

  藤村 知賢, 板野 理, 松田 祐子, 北郷 実, 篠田 昌宏, 田邉 稔, 荻野 千秋, 清水 宣明, 北川 雄光

  (一社)日本消化器外科学会, Jul. 2013, 日本消化器外科学会総会, 68回, O - 82, Japanese


• Simultaneous Saccharification and Fermentation of Ethanol from Ionic Liquid Treated Biomass

  荻野 千秋, 山田 亮祐, 近藤 昭彦

  小峰工業出版, Feb. 2013, 化學工業, 64(2) (2), 136 - 139, Japanese


• 1P-118 Metabolic engineering for homogentisic acid production in Streptomyces lividans

  Niimi-Nakamura Satoko, Kawaguchi Hideo, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 47 - 47, Japanese


• 1P-105 Simultaneous saccharification and fermentation of cellulose for phenyllactic acid production

  Kawaguchi Hideo, Niimi-Nakamura Satoko, Ogino Chiaki, Oinuma Kenichi, Takaya Naoki, Kondo Akihiko

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 44 - 44, Japanese


• 1P-117 Production of protocatechuate by Corynebacterium glutamicum

  Okai Naoko, Takeshima Yasunobu, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 47 - 47, Japanese


• 1P-183 Simultaneous saccharification and fermentation from lignocellulosic material pretreated by biocompatible ionic liquid

  Omote Sayuri, Ninomiya Kazuaki, Soda Hiroshi, Ogino Chiaki, Takahashi Kenji, Shimizu Nobuaki

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 63 - 63, Japanese


• 1P-218 Pretreatment of lignocellulosic biomass by acid-catalysed process in aqueous ionic liquid solution

  Ogura Kazuma, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 72 - 72, Japanese


• 2P-149 Proposal of a cost-effective bioethanol production process using commercial ionic liquids

  Saitoh Satoshi, Ishida Nobuhiro, Kamiya Noriho, Ogino Chiaki

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 141 - 141, Japanese


• 2P-148 Sugar concentration in liquid hydrolysate of dilute acid pretreated rice straw by nanofiltration

  Sasaki Kengo, Hasunuma Tomohisa, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 141 - 141, Japanese


• 3P-174 Development of affibody-displaying bio-nanocapsule for specific delivery to EGFR-expressing cells

  Ezawa Ryosuke, Nishimura Yuya, Ishii Jun, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 231 - 231, Japanese


• 神戸大学大学院工学研究科応用化学専攻 生物化学工学研究室

  近藤 昭彦, 荻野 千秋, 田中 勉, 蓮沼 誠久, 石井 純, 原 清敬, 山田 亮祐, 岡井 直子, 工藤 基徳, 佐々木 建吾, 川口 秀夫, 柘植 陽太

  05 Nov. 2012, 化学工学 = Chemical engineering, 76(11) (11), 722 - 722, Japanese


• Novel Radiosensitization Through Reactive Oxygen Species Generation Using Titanium Peroxide Nanoparticle Compounds Against Pancreas Cancer

  M. Nakayama, R. Sasaki, C. Ogino, T. Tanaka, M. Umetsu, S. Ohara, Z. Tan, K. Sato, C. Numako, A. Kondo

  Nov. 2012, INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 84(3) (3), S700 - S700, English

  Summary international conference


• Benzoic acid fermentation from starch and cellulose via a plant-like beta-oxidation pathway in Streptomyces maritimus

  Shuhei Noda, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Sep. 2012, NEW BIOTECHNOLOGY, 29, S50 - S50, English

  Summary international conference


• ナイチノールステント・超音波触媒法による再閉塞予防の検討

  藤村 知賢, 板野 理, 松田 祐子, 北郷 実, 篠田 昌宏, 河地 茂行, 田邉 稔, 荻野 千秋, 清水 宣明, 北川 雄光

  (一社)日本消化器外科学会, Jul. 2012, 日本消化器外科学会総会, 67回, 1 - 1, Japanese


• 外科領域における先端技術・治療の開発 酸化チタン・超音波触媒法によるラジカル生成を利用した肝胆膵悪性腫瘍に対する医工融合型治療法の開発

  板野 理, 松田 祐子, 大西 達也, 藤村 知賢, 北郷 実, 篠田 昌宏, 河地 茂行, 神野 浩光, 田邉 稔, 荻野 千秋, 清水 宣明, 北川 雄光

  (一社)日本外科学会, Mar. 2012, 日本外科学会雑誌, 113(臨増2) (臨増2), 126 - 126, Japanese


• ラジカル生成を利用した胆管ステント再狭窄予防の検討

  藤村 知賢, 板野 理, 松田 祐子, 大西 達也, 北郷 実, 篠田 昌宏, 河地 茂行, 神野 浩光, 田邉 稔, 荻野 千秋, 清水 宣明, 北川 雄光

  (一社)日本外科学会, Mar. 2012, 日本外科学会雑誌, 113(臨増2) (臨増2), 389 - 389, Japanese


• 4Ip22 Saccharification and fermentation of biomass by the ionic liquid pretreatment with the engineered xylose fermentable yeast

  Ogura Kazuma, Nakashima Nanami, Yamada Ryousuke, Hasunuma Tomohisa, Kamiya Noriho, Ishida Nobuhiro, Saito Satosi, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 248 - 248, Japanese


• 4Ia02 Ethanol production from woody biomass by ionic liquid pretreatment

  Ishida Nobuhiro, Katahira Satoshi, Saito Satoshi, Kamiya Noriho, Ogino Chiaki

  日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 239 - 239, Japanese


• 4Ha05 Construction of biomass-degradation enzymes library from Streptomyces and exploring novel high-level secretion signal

  Miyazaki Takaya, Noda Shuhei, Tanaka Tsutomu, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 230 - 230, Japanese


• 4Ba09 Characterization of DNA aptamer selected by Cell SELEX for human hepatic cancer cells

  Kawashima Satoshi, Ninomiya Kazuaki, Ogino Chiaki, Shimizu Nobuaki

  日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 186 - 186, Japanese


• 4Dp13 Benzoic acid fermentation from starch and cellulose using the polyketide synthesis pathway of Streptomyces maritimus

  Noda Shuhei, Tanaka Tsutomu, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 208 - 208, Japanese


• 4Dp02 Production of cadaverine by Corynebacterium glutamicum

  Okai Naoko, Niba Emma, Nakayama Hideki, Matsuda Fumio, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 205 - 205, Japanese


• イオン液体耐性セルラーゼの単離と酵素特性解析

  窪田有華, 工藤基徳, 仲島菜々実, 中島一紀, 岡崎文美, 荻野千秋, 近藤昭彦

  2012, 酵素工学研究会講演会講演要旨集, 68th


• イオン液体耐性セルラーゼの探索と機能解析

  窪田有華, 山口剛示, 仲島菜々実, 工藤基徳, 中島一紀, 岡崎文美, 荻野千秋, 近藤昭彦

  2012, 日本生物工学会大会講演要旨集, 64th


• 海洋性超好熱菌Thermotoga neapolitana由来β-1,3-キシラナーゼの耐熱性を導く要因の解析

  山崎清志, 岡崎文美, 荻野千秋, 近藤昭彦, 三上文三, 榑林陽一, 鶴田宏樹

  2012, 日本農芸化学会大会講演要旨集(Web), 2012


• 海洋性超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの構造安定性

  岡崎文美, 仲島菜々実, 荻野千秋, 近藤昭彦

  2012, 日本農芸化学会大会講演要旨集(Web), 2012


• 麹菌Aspergillus oryzaeを用いたラクダ科動物由来一本鎖抗体可変部位V_HH分泌生産系の構築

  吉栄俊秀, 青木淳一, 田淵聡一郎, 岡崎文美, 荻野千秋, 田中勉, 久田博元, 秦洋二, 近藤昭彦

  2012, 日本農芸化学会大会講演要旨集(Web), 2012


• 超音波と酸化チタンを用いた音響力学療法の基礎的検討

  大西 達也, 松田 祐子, 板野 理, 神野 浩光, 荻野 千秋, 清水 宣明, 北川 雄光

  (一社)日本癌治療学会, Sep. 2011, 日本癌治療学会誌, 46(2) (2), 1046 - 1046, Japanese


• Streptomyces griseocarneusスフィンゴミエリナーゼCの速度論的解析とMg²⁺による活性増強メカニズムの解明

  冨田優, 松本優作, 杉森大助, 村山和隆, 荻野千秋

  05 Mar. 2011, 日本農芸化学会大会講演要旨集, 2011, 47, Japanese


• 1Kp11 IPA production from cellobiose by E. coli displayed BGL

  Souma Yuki, Inokuma Kentaro, Tanaka Tsutomu, Ogino Chiaki, Kondo Akihiko, Hanai Taizo

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 69 - 69, Japanese


• 1Lp01 Application of bio-molecule modified nano-particle in biochemical engineering

  Ogino Chiaki

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 71 - 71, Japanese


• 1Ap14 Development of ionic liquid-based consolidated bioprocessing (i-CBP)

  OGINO Chiaki, NAKASHIMA Kazunori, YAMAGUCHI Koji, KUBOTA Yuka, KATAHIRA Satoshi, ISHIDA Nobuhiro, TOKUHARA Wataru, NORITAKE Yoshiyuki, KONDO Akihiko

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 24 - 24, Japanese


• 1Bp17 Direct Glutathione Fermentation from Biomass by Engineered Yeast

  Hara Kiyotaka, Yoshida Hideyo, Kiriyama Kentaro, Kim Songhee, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 28 - 28, Japanese


• 1Ap13 Ethanol production from ionic liquid pretreated biomass with cellulase expressing yeast

  Katahira Satoshi, Nakamura Risa, Tokuhara Wataru, Noritake Yoshiyuki, Nakashima Kazunori, Kamiya Noriho, Ogino Chiaki, Ishida Nobuhiro

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 23 - 23, Japanese


• 1Ap12 Effect of ionic liquid pretreatment of crystalline cellulose on enzymatic saccharification efficiency

  Nakmoto Aya, Uju Uju, Shoda Yasuhiro, Goto Masahiro, Ishida Nobuhiro, Tokuhara Wataru, Noritake Yoshiyuki, Ogino Chiaki, Kamiya Noriho

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 23 - 23, Japanese


• 2Ip12 Efficient glutathione production by novel recombinant yeast containing high intracellular glutathione

  kiriyama kentaro, hara kiyotaka, nakayama hideki, ogino chiaki, kondo akihiko

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 179 - 179, Japanese


• 1Ep12 Production of thermostable β-1,4-mannanase from hyperthermophilic eubacterium Thermotoga neapolitana by Aspergillus oryxae

  OKAZAKI Fumiyoshi, NAKASHIMA Nanami, HISADA Hiromoto, OGINO Chiaki, ISHIDA Hiroki, Yopi, HATA Yoji, KONDO Akihiko

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 42 - 42, Japanese


• 2Lp13 Characterization of DNA aptamer selected by Cell-SELEX for human hepatic cancer cells

  Iwakura Kazuki, Kawashima Satoshi, Ninomiya Kazuaki, Ogino Chiaki, Shimizu Nobuaki

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 201 - 201, Japanese


• 麹菌Aspergillus oryzaeを用いたラクダ由来一本鎖抗体可変部位V_HH分泌生産系の構築

  青木淳一, 田淵聡一郎, 岡崎文美, 荻野千秋, 田中勉, 久田博元, 秦洋二, 近藤昭彦

  2011, 日本農芸化学会関西支部講演会講演要旨集, 472nd


• 麹菌Aspergillus oryzaeによる超好熱菌Thermotoga neapolitana由来耐熱性β-1,4-マンナナーゼの生産

  岡崎文美, 仲島菜々実, 久田博元, 荻野千秋, 石田博樹, YOPI, 秦洋二, 近藤昭彦

  2011, 日本生物工学会大会講演要旨集, 63rd


• Affibody提示Bio-nanocapsuleを用いたHER2発現癌細胞へのタンパク質送達システム

  西村勇哉, 三村和起子, 竹田光一, 石井純, 岡崎文美, 荻野千秋, 近藤昭彦

  2011, 日本生物工学会大会講演要旨集, 63rd


• 麹菌Aspergillus oryzaeを用いたラクダ由来一本鎖抗体可変部位V_HHの生産

  青木淳一, 田淵聡一郎, 岡崎文美, 荻野千秋, 田中勉, 久田博元, 秦洋二, 近藤昭彦

  2011, 日本生物工学会大会講演要旨集, 63rd


• 2Ip13 Affibody displaying Bio-nanocapsule for protein therapy to HER2 expressing cancer cells

  Nishimura Yuya, Mimura Wakiko, Takeda Koichi, Ishii Jun, Okazaki Fumiyoshi, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 180 - 180, Japanese


• Innovative BioProduction Kobe (iBioK) Project in Kobe University

  近藤 昭彦, 荻野 千秋

  フレグランスジャーナル社, Oct. 2010, Fragrance journal, 38(10) (10), 39 - 44, Japanese


• Streptomyces griseocarneus NBRC13471由来スフィンゴミエリナーゼCの大量発現

  冨田優, 杉森大助, 荻野千秋, 松本優作

  25 Sep. 2010, 日本生物工学会大会講演要旨集, 62nd, 30, Japanese


• 2P-1085 Overexpression of shingomyelinase C from Streptomyces griseocarneus NBRC13471

  TOMITA Yu, SUGIMORI Daisuke, OGINO Chiaki, MATSUMOTO Yusaku

  公益社団法人日本生物工学会, 25 Sep. 2010, 日本生物工学会大会講演要旨集, 22, 30 - 30, Japanese


• 2P-1086 Overexpression and kinetics of sphingomyelinase C from Streptomyces griseocarneus NBRC13471

  MATUMOTO Yusaku, TOMITA Yu, SUGIMORI Daisuke, OGINO Chiaki

  公益社団法人日本生物工学会, 25 Sep. 2010, 日本生物工学会大会講演要旨集, 22, 30 - 30, Japanese


• 2P-1106 Over-production of various secretory-form proteins in Streptomyces lividans

  NODA Shuhei, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 35 - 35, Japanese


• 2B-Ba05 Single-cell analysis and agonist screening for G-protein-coupled receptors

  Kondo Akihiko, Ishii Jun, Ogino Chiaki

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 197 - 197, Japanese


• 3P-2082 Development of non-invasive cancer cell killing method based on combination of titanium peroxiside nano-particle and low intensity X-ray irradiation

  KARASAKI Miki, MATSUI Kazusa, SATO Kazuyoshi, OHARA Satoshi, SASAKI Ryohei, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 168 - 168, Japanese


• 3P-2043 Development of ionic liquid-based consolidated bioprocessing (i-CBP), part. 3.

  YAMAGUCHI Koji, NAKASHIMA Kazunori, YAMADA Ryosuke, OGINO Chiaki, KONDO Akihiko

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 154 - 154, Japanese


• 3P-2084 Selection of DNA aptamer for human hepatic cancer cells by Cell SELEX

  NINOMIYA Kazuaki, KANEDA Kazuhiko, OGINO Chiaki, SHIMIZU Nobuaki

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 169 - 169, Japanese


• 3P-1180 Affibody displaying Bionanocapsules for specific drug delivery to HER2 expressing cancer cell

  MIEDA Hiroaki, SHISHIDO Takuya, NISHIMURA Yuya, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 102 - 102, Japanese


• 2P-2047 Direct ethanol production from cellulose by using cellulolytic enzymes expression optimized yeast strain

  YAMADA Ryosuke, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 118 - 118, Japanese


• 3S-Da03 Construction of biomaterial conjugated nano-particles for environmental and medical application.

  OGINO Chiaki

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 233 - 233, Japanese


• 超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの機能解析

  岡崎文美, 仲島菜々実, 久田博元, 荻野千秋, 石田博樹, 秦洋二, 近藤昭彦

  2010, 日本生物工学会大会講演要旨集, 62nd


• 麹菌による超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの高分泌生産

  久田博元, 岡崎文美, 石田博樹, 荻野千秋, 秦洋二, 近藤昭彦

  2010, 日本生物工学会大会講演要旨集, 62nd


• 麹菌(Aspergillus oryzae)を宿主とした超好熱菌Thermotoga neapollitana由来耐熱性β-1,3-キシラナーゼの分泌高生産

  久田博元, 岡崎文美, 石田博樹, 荻野千秋, 秦洋二, 近藤昭彦

  2010, Journal of Applied Glycoscience, 57(Suppl.) (Suppl.)


• 超好熱菌Thermotoga neapollitana由来耐熱性β-1,3-キシラナーゼの発現および諸性質

  岡崎文美, 仲島菜々実, 久田博元, 荻野千秋, 石田博樹, 秦洋二, 近藤昭彦

  2010, Journal of Applied Glycoscience, 57(Suppl.) (Suppl.)


• BP-P10 Affibody displaying bionanocapsules for HER2 specific drug delivery(Section II Biopharmaceuticals Production)

  Nishimura Yuya, Shishido Takuya, Mieda Hiroaki, Tanaka Tsutomu, Ogino Chiaki, Kondo Akihiko

  The Society for Biotechnology, Japan, Nov. 2009, Journal of bioscience and bioengineering, 108(1) (1), S27, English


• BE-O21 New SELEX strategy for screening of DNA aptamer by AFM(Section V Biomolecular Engineering and Bioseparation)

  Miyachi Yusuke, Shimizu Nobuaki, Ogino Chiaki, Kondo Akihiko

  The Society for Biotechnology, Japan, Nov. 2009, Journal of bioscience and bioengineering, 108(1) (1), S64, English


• BE-P21 Screening of DNA aptamers that recognize amino acid by AFM-SELEX strategy(Section V Biomolecular Engineering and Bioseparation)

  Hayase Taiji, Miyachi Yusuke, Ogino Chiaki, Kondo Akihiko

  The Society for Biotechnology, Japan, Nov. 2009, Journal of bioscience and bioengineering, 108(1) (1), S71, English


• New SELEX strategy for screening of DNA aptamer by AFM

  Yusuke Miyachi, Nobuaki Shimizu, Chiaki Ogino, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S64 - S64, English

  Summary international conference


• Development of novel cell-surface display system of Aspergillus oryzae using chitin binding domain

  Soichiro Tabuchi, Junji Ito, Takashi Adachi, Hiroki Ishida, Yoji Hata, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S50 - S50, English

  Summary international conference


• Affibody displaying bionanocapsules for HER2 specific drug delivery

  Yuya Nishimura, Takuya Shishido, Hiroaki Mieda, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S27 - S27, English

  Summary international conference


• Efficient and practical ethanol production from high yield rice by amylase expressing Saccharomyces cerevisiae

  Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S50 - S50, English

  Summary international conference


• Efficient D-lactic acid production from raw starch

  Satoru Shinkawa, Kenji Okano, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S47 - S48, English

  Summary international conference


• Investigation of the interaction between GPCR and ligand by AFM equipped with bio-molecule modified cantilever

  Kazuki Nosaka, Yusuke Miyachi, Jun Ishii, Chiaki Ogino, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S159 - S160, English

  Summary international conference


• Efficient homo D-lactic acid production from xylose

  Shogo Yoshida, Kenji Okano, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S48 - S48, English

  Summary international conference


• Biodiesel production using whole-cell biocatalyst of Aspergillus oryzae coexpression lipases

  Daisuke Adachi, Kazunori Nakashima, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo, Hideki Fukuda

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S51 - S52, English

  Summary international conference


• Efficient repeated batch fermentation from raw starch by novel yeast expressing transporter gene and amylase genes

  Syunichi Yamakawa, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S51 - S51, English

  Summary international conference


• Efficient ethanol production from xylose by mated diploid Saccharomyces cerevisiae

  Hiroaki Suyama, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S49 - S49, English

  Summary international conference


• Engineering of endoglucanase expression system for Corynebacterium glutamicum

  Takeyuki Tsuchidate, Toshihiro Tateno, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S48 - S49, English

  Summary international conference


• Direct fermentation of cellulosic materials to ethanol using yeast strains codisplaying three types of cellulolytic enzyme

  Shuhei Yanase, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S52 - S52, English

  Summary international conference


• Antibody-immobilized TiO2 nanoparticles for cancer therapy

  Kazusa Matsui, Maiko Segawa, Tsutomu Tanaka, Akihiko Kondo, Chiaki Ogino

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S36 - S37, English

  Summary international conference


• Screening of DNA aptamers that recognize amino acid by AFM-SELEX strategy

  Taiji Hayase, Yusuke Miyachi, Chiaki Ogino, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S71 - S71, English

  Summary international conference


• Production of biodiesel fuel in ionic liquids catalyzed by whole-cell biocatalysts

  Kazunori Nakashima, Shogo Arai, Takanori Tanino, Chiaki Ogino, Akihiko Kondo, Hideki Fukuda

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S43 - S43, English

  [Refereed]

  Summary international conference


• Bioethaol fermentation from mixed sugar by the recombinant yeast with xyloseisomerase pathway

  Takanori Tanino, Atsushi Hotta, Tomonori Ito, Jun Ishii, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Naoto Ohmura, Takayuki Ohshima, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S53 - S53, English

  [Refereed]

  Summary international conference


• Bioethanol production from mixed sugars using sugar uptake ability enhanced yeast strain by overexpression of transporters

  Atsushi Hotta, Takanori Tanino, Tomonori Ito, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo, Naoto Ohmura

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S53 - S53, English

  [Refereed]

  Summary international conference


• Cancer therapy based on sonocatalytic radical generator from photocatalytic nano-particle

  仁宮 一章, 清水 宣明, 荻野 千秋

  ティー・アイ・シィー, Jun. 2009, Materials integration, 22(6) (6), 8 - 11, Japanese


• 1Ep22 Development of Affibody displaying Bio-nanocapsule for targeted cancer therapy

  MIEDA Hiroaki, SHISHIDO Takuya, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  日本生物工学会, 2009, 日本生物工学会大会講演要旨集, 21, 57 - 57, Japanese


• 2La01 Efficient ethanol production from raw starch using novel yeast strain

  Yamakawa Syunichi, Yamada Ryosuke, Tanaka Tutomu, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2009, 日本生物工学会大会講演要旨集, 21, 170 - 170, Japanese


• 2La11 Development of novel cell-surface display system using porin in Corynebacterium glutamicum

  Hatada Kazuki, Tateno Toshihiro, Okai Naoko, Tanaka Tsutomu, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2009, 日本生物工学会大会講演要旨集, 21, 172 - 172, Japanese


• 2Lp02 Efficient protein production in recombinant Aspergillus oryzae

  SAKAI Shoji, MATSUDA Tetsuya, ADACHI Takashi, HISADA Hiromoto, HATA Yoji, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  日本生物工学会, 2009, 日本生物工学会大会講演要旨集, 21, 173 - 173, Japanese


• 二酸化チタン/超音波触媒法とその医療応用

  清水 宣明, 仁宮 一章, 荻野 千秋

  化学工学会, 05 Nov. 2008, 化学工学 = Chemical engineering, 72(11) (11), 636 - 638, Japanese


• Streptomyces griseocameus NBRC13471由来スフィンゴミエリナーゼC遺伝子のクローニングと発現

  杉森大助, 冨田優, 荻野千秋, 劉暁麗

  05 Nov. 2008, 石油・石油化学討論会講演要旨, 38th, 211, Japanese


• 特異性改変型バイオナノカプセルを用いたバイオイメージング及びDDSへの応用

  近藤 昭彦, 宍戸 卓矢, 田中 勉, 荻野 千秋

  30 Oct. 2008, バイオイメージング, 17(2) (2), 110 - 111, Japanese


• ORGN 297-Phospholipid conversion in ionic liquid by phospholipase D

  Chiaki Ogino, Toru Miyashita, Nobuaki Shimizu

  Aug. 2008, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 236, English

  Summary international conference


• Pinpoint delivery of TiO２ nano-particles to the cancer cells in vitro

  笹井 良祐, 荻野 千秋, 清水 宣明

  金沢大学環日本海域環境研究センター = Institute of Nature and Environmental Technology, Kanazawa University, Japan, 31 Jul. 2008, 年報 金沢大学環日本海域環境研究センター = K-INET Kanazawa University, 2007, 129 - 134, Japanese


• Sonocatalytic formation of H2O2 following sonication of water in the presence of TiO2

  崎山 和亨, 荻野 千秋, 清水 宣明

  金沢大学環日本海域環境研究センター = Institute of Nature and Environmental Technology, Kanazawa University, Japan, 31 Jul. 2008, 年報 金沢大学環日本海域環境研究センター = K-INET Kanazawa University, 2007, 135 - 140, Japanese


• Properties of TiO2-polyacrylic acid dispersions with potential for molecular recognition

  Koki Kanehira, Toshiaki Banzai, Chiaki Ogino, Nobuaki Shimizu, Yoshinobu Kubota, Shuji Sonezaki

  Jun. 2008, COLLOIDS AND SURFACES B-BIOINTERFACES, 64(1) (1), 10 - 15, English


• 微生物を用いたバイオ・リファイナリー戦略--バイオマスから燃料・化学品の生産 (特集 バイオマス利用技術の展望)

  荻野 千秋, 近藤 昭彦

  化学工業社, Feb. 2008, ケミカルエンジニヤリング, 53(2) (2), 140 - 144, Japanese


• 2Gp20 Construction of the cellulase expression system in Streptomyces lividans

  ITO Yuko, MIYASHITA Toru, SHIMIZU Nobuaki, OGINO Chiaki

  日本生物工学会, 2008, 日本生物工学会大会講演要旨集, 20, 184 - 184, Japanese


• 1Fp15 Gene cloning and expression of sphingomyelinase C from Streptomyces griseocarneus NBRC13471

  SUGIMORI Daisuke, TOMITA Yu, OGINO Chiaki, LIU Xiaoli

  日本生物工学会, 2008, 日本生物工学会大会講演要旨集, 20, 156 - 156, Japanese


• 2Fa01 Phospholipid Conversion in Ionic Liquid by Phospholipase D

  MIYASHITA Toru, ITO Yuko, SHIMIZU Nobuaki, OGINO Chiaki

  日本生物工学会, 2008, 日本生物工学会大会講演要旨集, 20, 158 - 158, Japanese


• 2Aa08 Efficient ethanol production from raw starch by delta-integrant polyploid fusants

  YAMADA Ryosuke, TANAKA Tsutomu, OGINO Chiaki, FUKUDA Hideki, KONDO Akihiko

  日本生物工学会, 2008, 日本生物工学会大会講演要旨集, 20, 60 - 60, Japanese


• Streptomyces griseocarneus NBRC13471由来スフィンゴミエリナーゼCの遺伝子クローニングと発現

  冨田優, 杉森大助, 荻野千秋, 劉暁麗

  2008, 酵素工学研究会講演会講演要旨集, 60th, 71, Japanese


• Gene cloning and expression of sphingomyelinase C from Streptomyces griseocarneus NBRC13471

  SUGIMORI DAISUKE, TOMITA YU, OGINO CHIAKI, Liu Xiaoli

  <i>S. griseocarneus</i> NBRC13471スフィンゴミエリナーゼC遺伝子<i>smc</i>をクローニングした。次に、発現ベクターpUC702に<i>smc</i>を導入し、<i>Streptoverticillium cinnamoneum</i>を宿主として発現を行った。その結果、形質転換体の培養上清液中に約100 mg/mlの本酵素を分泌生産させることができた。しかしながら、培養上清中の酵素比活性は3 mU/mgと元株の約100分の１であった。

  公益社団法人 石油学会, 2008, Proceeding of Annual/Fall Meetings of the Japan Petroleum Institute, 2008(0) (0), 142 - 142, Japanese


• 二酸化チタン・超音波触媒法による細胞死誘導 (特集 ソノケミストリーの最新動向(2))

  高木 圭子, 荻野 千秋, 清水 宣明

  日本工業出版, Sep. 2007, 超音波techno, 19(5) (5), 41 - 45, Japanese


• Induction of astaxanthin biosynthesis in H-pluvialis using TiO2 and ultrasounds

  Tomohisa Katsuda, Chiaki Ogino, Keisuke Yamagami, Shigeo Katoh, Nobuaki Shimizu

  Sep. 2007, JOURNAL OF BIOTECHNOLOGY, 131(2) (2), S185 - S186, English

  Summary international conference


• Over-expression system for secretory phospholipase D by Streptomyces lividans

  Chiaki Ogino, Takafumi Kubo, Kazumichi Yoshino, Nobuaki Shimizu

  Sep. 2007, JOURNAL OF BIOTECHNOLOGY, 131(2) (2), S237 - S238, English

  Summary international conference


• 二酸化チタン・超音波触媒法を応用した有害微生物殺菌システム (特集 ソノケミストリーの最新動向(1))

  荻野 千秋, Rahman Mohammad Mizanur, 清水 宣明

  日本工業出版, Jul. 2007, 超音波techno, 19(4) (4), 45 - 49, Japanese


• 二酸化チタン・超音波触媒法によるOHラジカルの生成と溶存希ガスの促進効果 (特集 ソノケミストリーの最新動向(1))

  清水 宣明, 荻野 千秋, 藤平 敦司

  日本工業出版, Jul. 2007, 超音波techno, 19(4) (4), 39 - 44, Japanese


• Formation of OH radicals during ultrasonic irradiation with Ti02 and its application to the elimination of hazardous chemicals and bacteria

  清水 宣明, 荻野 千秋

  日本空気清浄協会, 2007, Journal of Japan Air Cleaning Association, 45(1) (1), 24 - 31, Japanese


• 2J11-1 Effect of signal peptide sequence on secretory protein production in Streptomyces lividans

  YOSHINO Takamichi, ITOU Yuko, OGINO Chiaki, SHIMIZU Nobuaki

  日本生物工学会, 2007, 日本生物工学会大会講演要旨集, 19, 213 - 213, Japanese


• 2C10-3 Mutation analysis of phospholipid methabolite enzyme by Streptmyces as a host strain

  ITOU Yoshiki, MIYASHITA Toru, OGINO Chiaki, SHIMIZU Nobuaki

  日本生物工学会, 2007, 日本生物工学会大会講演要旨集, 19, 82 - 82, Japanese


• 2H12-5 Interaction analysis of DNA aptamer that recognizes 8-hydroxy-2-deoxyguanosine

  MIYACHI Yusuke, ITOU Yuko, OGINO Chiaki, SHIMIZU Nobuaki

  日本生物工学会, 2006, 日本生物工学会大会講演要旨集, 18, 142 - 142, Japanese


• 2K12-4 Intracellular uptake of TiO_2 nanoparticles and sonodynamic injury of tumor cell membrane

  TAKAKI Keiko, OGINO Chiaki, SHIMIZU Nobuaki

  日本生物工学会, 2006, 日本生物工学会大会講演要旨集, 18, 202 - 202, Japanese


• 2K12-5 Synthesis and modification of fusogenic liposome encapsulated with TiO_2 nanoparticles

  OGINO Chiaki, TAKAKI Keiko, SHIMIZU Nobuaki

  日本生物工学会, 2006, 日本生物工学会大会講演要旨集, 18, 203 - 203, Japanese


• 1L10-5 Investigation of recombinant production of phospholipase D by Streptomyces

  KUBO Takafumi, YOSHINO Takamichi, ITOU Yoshiki, MIYASHITA Tooru, OGINO Chiaki, SHIMIZU Nobuaki

  日本生物工学会, 2006, 日本生物工学会大会講演要旨集, 18, 212 - 212, Japanese


• 二酸化チタンへの超音波照射によって発生するラジカルの応用 (特集 いろいろつかえるソノケミストリー(1))

  清水 宣明, 荻野 千秋, Dadjour Mahmoud Farshbaf

  ティ-・アイ・シ-, Mar. 2005, マテリアルインテグレ-ション, 18(3) (3), 31 - 36, Japanese


• 二酸化チタン・超音波照射法による大腸菌殺菌メカニズム (特集/ソノケミストリーの新しい進展)

  清水 宣明, 荻野 千秋, Dadjour Mahmoud Farshbaf

  化学工業社, Feb. 2005, 化学工業, 56(2) (2), 106 - 111, Japanese


• 2E11-2 Effect of lid structure of phospholipase D on transphosphatidylation activity

  OHISHI Takako, TAKADA Namiko, ITOU Yoshiki, OGINO Chiaki, SHIMIZU Nobuaki

  日本生物工学会, 2005, 日本生物工学会大会講演要旨集, 17, 153 - 153, Japanese


• 2E10-3 Construction of recombinant protein expression system by using the promoter region from Streptomyces cinnamoneum

  KUBO Takafumi, YOSHINO Takamichi, OGINO Chiaki, SHIMIZU Nobuaki

  日本生物工学会, 2005, 日本生物工学会大会講演要旨集, 17, 152 - 152, Japanese


• Production of phospholipase D and its application for phospholipid synthesis

  OGINO Chiaki, FUKUDA Hideki

  バイオインダストリー協会, 01 Dec. 2004, Bioscience & industry, 62(12) (12), 809 - 812, Japanese


• 3C11-4 Screening and characterization of nucleotide recognized DNA aptamer

  OGINO Chiaki, MURATA Tomoyuki, SHIMIZU Nobuaki

  日本生物工学会, 2004, 日本生物工学会大会講演要旨集, 16, 146 - 146, Japanese


• Characterization of mutants phospholipase D from Streptoverticillium cinnamoneum

  Ogino Chiaki, Izumi Mariko, Ohishi Takako, Shimizu Nobuaki

  日本生物工学会, 2003, 日本生物工学会大会講演要旨集, 15, 71 - 71, Japanese


• 927 Biosensing of benzene derivatives with Ps promoter gene and reporter protein GFP

  Ikeno Shinya, Ito Takeo, Sugino Yasuhiro, Ogino Chiaki, Shimizu Nobuaki

  日本生物工学会, 2002, 日本生物工学会大会講演要旨集, 14, 171 - 171, Japanese


• 672 Catalytic function analysis of phospholipase D by SiteDirect Mutagenesis

  Ogino Chiaki, Hatanaka Yuki, Izumi Mariko, Shimizu Nobuaki

  日本生物工学会, 2002, 日本生物工学会大会講演要旨集, 14, 112 - 112, Japanese


• Construction and Investigation of Mutant Phospholipase D from Streptoverticillium cinnamoneum

  Ogino Chiaki, Oomura Yuka, Shimizu Nobuaki, Kanemasu Masayuki, Kondo Akihiko, Fukuda Hideki

  日本生物工学会, 2000, 日本生物工学会大会講演要旨集, 12, 183 - 183, Japanese


• Expression and characterization of fused PLDα from Stv. cinnamoneum in E. coli.

  Daido Hidenori, Ogino Chiaki, Kanemasu Masayuki, Kondo Akihiko, Fukuda Hideki

  日本生物工学会, 1999, 日本生物工学会大会講演要旨集, 11, 92 - 92, Japanese


• Purification and characterization of a novel Phospholipase D from Streptoverticillium cinnamoneum

  Ogino Chiaki, Nakaoka Kouichi, Matsumiya Toshiko, Daido Hidenori, Kondo Akihiko, Fukuda Hideki

  日本生物工学会, 1998, 日本生物工学会大会講演要旨集, 10, 166 - 166, Japanese


• Purification and cloning of Phospholpiase-D from Streptoverticllium cinnamoneum

  Ogino Chiaki, Matumiya Toshiko, Tokuyama Shinji, Kuroda Shun'ichi, Kondou Akihiko, Fukuda Hideki

  日本生物工学会, 1997, 日本生物工学会大会講演要旨集, 9, 134 - 134, Japanese


• Study on PLD of actinomycete for signal transduction

  Ogino Chiaki, Negi Yukinari, Kondo Akihiko, Fukuda Hideki

  日本生物工学会, 1996, 日本生物工学会大会講演要旨集, 8, 189 - 189, Japanese

• Yeast Cell Surface Engineering : "Energy Production: Biodiesel"

  OGINO CHIAKI, AMOAH JEROME

  Joint work, Springer Nature, May 2019, English

  Scholarly book


• A cancer treatment strategy that combines the use of inorganic/biocomplex nanoparticles with conventional radiation therapy, Nanoparticle Technology Handbook

  MORITA Kenta, NISHIMURA Yuya, SUZUKI T, OGINO Chiaki, KONDO Akihiko

  Joint work, Elsevier, Mar. 2018, English

  Scholarly book


• Green Processes for Nanotechnology

  SRIVASTAVA S.K, OGINO Chiaki, KONDO Akihiko

  Joint work, Springer, Apr. 2015, English

  Scholarly book


• 紙パルプ産業と環境 2015

  KONDO AKIHIKO, OGINO CHIAKI

  Joint work, テックタイムス, Aug. 2014, Japanese

  Scholarly book


• 化学便覧 応用化学編

  KONDO AKIHIKO, OGINO CHIAKI, HASUNUMA TOMOHISA, ISHII JUN, HARA KIYOTAKA, OKAI NAOKO, 山田 亮祐

  Joint work, 丸善出版, Jan. 2014, Japanese

  Scholarly book


• 基礎から学ぶ生物化学工学演習

  OGINO CHIAKI

  Joint work, コロナ社, Sep. 2013, Japanese

  Scholarly book


• リサイクルバイオテクノロジーの最前線, 第I編 第1章, 1 未来型CBP法によるバイオエタノール生産技術の展開

  HASUNUMA TOMOHISA, OGINO CHIAKI, KONDO AKIHIKO

  Joint work, シーエムシー出版, May 2013, Japanese

  Scholarly book


• 次世代医薬開発に向けた抗体工学の最前線 （第Ⅱ編 抗体の改変技術，第4章 親和性の向上，第4節 「酵母による抗体フラグメントおよび抗体様結合性タンパク質の改変技術」）

  ISHII JUN, OGINO CHIAKI, KONDO AKIHIKO

  Joint work, シーエムシー出版, Dec. 2012, Japanese

  Scholarly book


• エコバイオリファイナリー

  KONDO Akihiko, OGINO Chiaki, HASUNUMA Tomohisa, TANAKA Tsutomu, NAKASHIMA Kazunori

  Joint work, シーエムシー出版, Dec. 2010, Japanese

  Scholarly book


• グリーンバイオケミストリーの最前線

  OGINO Chiaki, HASUNUMA Tomohisa, KONDO Akihiko

  Joint work, シーエムシー出版, Apr. 2010, Japanese

  Scholarly book


• シングルセル解析の最前線 （第３章 細胞内生体分子群の実測定量解析，第4節 「フローサイトメトリーとGFPレポーターによるG蛋白質シグナルのシングルセル解析」）

  石井 純, 田中 勉, 荻野 千秋, 近藤 昭彦

  Joint work, シーエムシー出版, Mar. 2010, Japanese

  Scholarly book


• 第二世代バイオ燃料の開発と応用展開

  近藤 昭彦, 福田 秀樹, 荻野 千秋, 田中 勉, 蓮沼 誠久, 原田 和生, 福崎 英一郎

  Joint work, シーエムシー出版, Apr. 2009, Japanese

  Scholarly book


• 微生物によるものづくり

  植田 充実, 芝崎 誠司, 荻野 千秋, 近藤 昭彦, 谷野 孝徳, 田中 勉, 福田 秀樹

  Single work, シーエムシー出版, Jun. 2008, Japanese

  Scholarly book


• 二酸化チタンと超音波で殺菌?!

  OGINO Chiaki

  Single work, 学進出版, Feb. 2008, Japanese

  Scholarly book


• 進化・増殖する化学工学

  荻野 千秋, 近藤 昭彦

  Joint work, 関西化学機械製作株式会社創立60周年記念出版, Feb. 2008, Japanese

  Scholarly book


• 微生物を用いたバイオ・リファイナリー戦略:バイオマスからの燃料・化学品の生産

  OGINO CHIAKI, KONDO AKIHIKO

  Joint work, ケミカルエンジニアリング, 2008, Japanese

  General book


• 二酸化チタン・超音波触媒法による細胞死誘導

  高木 圭子, OGINO Chiaki, 清水 宣明

  Joint work, 超音波テクノ, Sep. 2007, Japanese

  Scholarly book


• 二酸化チタン・超音波触媒法を応用した有害微生物殺菌システム

  OGINO Chiaki, Mohammad Mizanur Rahaman, 清水宣明

  Joint work, 超音波テクノ, Jul. 2007, Japanese

  Scholarly book


• 二酸化チタン・超音波触媒法によるOHラジカルの生成と溶存希ガスの促進効果

  清水 宣明, OGINO Chiaki, 藤平 敦司

  Joint work, 超音波テクノ, Jul. 2007, Japanese

  Scholarly book


• 二酸化チタン・超音波触媒法の環境浄化、殺菌への応用

  清水 宣明, OGINO Chiaki

  Joint work, 空気清浄, May 2007, Japanese

  Scholarly book


• ナノテクノロジーによる新規がん治療法.

  清水 宣明, OGINO Chiaki, 高木 圭子, 山岸 紗也花, 野瀬 律子

  Joint work, 最新医学, Jun. 2006, Japanese

  Scholarly book


• 二酸化チタンと超音波で殺菌?

  OGINO Chiaki

  Single work, 生物工学会誌, Jan. 2006, Japanese

  Scholarly book


• 超音波利用技術集成「超音波と二酸化チタンを用いた有害微生物の殺菌」

  清水 宣明, OGINO Chiaki

  Joint work, NTS, Apr. 2005, Japanese

  Scholarly book


• 二酸化チタンへの超音波照射によって発生するラジカルの応用

  清水 宣明, OGINO Chiaki, MF Dadjour

  Joint work, マテリアルインテグレーション, Mar. 2005, Japanese

  Scholarly book


• 二酸化チタン・超音波照射による大腸菌殺菌メカニズム

  清水 宣明, OGINO Chiaki, MF Dadjour

  Joint work, 化学工業, Feb. 2005, Japanese

  Scholarly book


• ホスホリパーゼDの生産とリン脂質合成への適用

  OGINO Chiaki, FUKUDA Hideki

  Joint work, バイオサイエンスとインダストリー, Dec. 2004, Japanese

  Scholarly book


• Stress-induced serotonin release in the hypothalamus and suppression of food intake in rats

  N. Shimizu, OGINO Chiaki

  Joint work, Recent Research Developments in Physiology, 2003, English

  Scholarly book


• テッシュエンジニアリングの展望

  紀ノ岡 正博, OGINO Chiaki

  Joint work, 三恵社, Mar. 2002, Japanese

  Scholarly book


• Functional analysis of phospholipase D and its application for phospholipids synthesis

  OGINO Chiaki

  Single work, INSEF, 2002, English

  Scholarly book


• エコエネルギー

  OGINO Chiaki

  Single work, 生物工学会誌, Jul. 2000, Japanese

  Scholarly book

• メタボローム解析が明らかにする低酸素濃度におけるコリネ型細菌の芳香族化合物代謝促進機構と代謝改変によるソルガム搾汁液からの物質生産

  川口 秀夫, 宮崎 翔子, 蓮沼 誠久, 荻野 千秋, 佐塚 隆志, 近藤 昭彦

  日本農芸化学会2020年度大会


• 油脂酵母Lipomyces starkeyi の遺伝子的改変による長鎖長脂肪酸の生産

  MIYAMOTO NAO, AMZA REZKY LASTINOV, PRIHARDI KAHAR, OGINO CHIAKI, KONDO AKIHIKO

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference

  Oral presentation


• 日本酒生産にヒントを得た麹菌酵素カクテルと酵母を用いた高純度希少糖生産

  若井 暁, 堤 浩子, 秦 洋二, OGINO CHIAKI, KONDO AKIHIKO

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference

  Oral presentation


• 新奇な好熱性放線菌Streptomyces thermoviolaceus を用いたタンパク質生産株の開発

  SAITO AKANE, MIHARA EMI, 曽田 匡洋, OGINO CHIAKI, KASHIWAGI NORIMASA, TAKENAKA MUSASHI, KONDO AKIHIKO

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference

  Oral presentation


• 酵母カルチャーコレクションからのバイオエタノール生産のための優良酵母の選抜と評価

  OGINO CHIAKI, PRIHARDI KAHAR, KONDO AKIHIKO

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference

  Oral presentation


• 好冷性海洋細菌Psychroflexus torquis 由来β-1,3-キシラナーゼの酵素化学的諸性質

  谷口 瑞季, 工藤 基徳, OGINO CHIAKI, KONDO AKIHIKO, 田丸 浩, 青木 恭彦, 岡﨑 文美

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference

  Oral presentation


• 好熱性放線菌Streptomyces thermoviolaceus による物質生産

  MIHARA EMI, TAKENAKA MUSASHI, KASHIWAGI NORIMASA, 曽田 匡洋, OGINO CHIAKI, KONDO AKIHIKO

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference

  Oral presentation


• 基質酵素反応予測のための 機械学習に基づく予測モデルの構築方法の探索と評価

  WATANABE NAOKI, MURATA MASAHIRO, OGINO CHIAKI, KONDO AKIHIKO, ARAKI MICHIHIRO

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference

  Oral presentation


• 過酸化チタンナノ粒子 (PAA -TiOx )の 細胞内傷害

  KOTERASAWA SHOTA, OGINO CHIAKI, NISHIMURA YUYA, KONDO AKIHIKO

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference

  Oral presentation


• イオン液体で前処理した木質系バイオマス中の多糖およびリグニンのリファイナリー

  廣部 綾乃, 柘植 陽太, OGINO CHIAKI, 高橋 憲司, 仁宮 一章

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference

  Oral presentation


• 新奇な好熱性放線菌Streptomyces thermoviolaceusを用いたタンパク質生産株の開発

  齊藤 朱音, 三原 笑, TAKENAKA MUSASHI, KASHIWAGI NORIMASA, OGINO CHIAKI, KONDO AKIHIKO

  第21回化学工学会学生発表会, Mar. 2019, Japanese, 京都大学桂キャンパス, Domestic conference

  Poster presentation


• 完全分散型麹菌のプロテオーム解析

  櫻川 拓, 片山 周平, Zhang Silai, WAKAI SATOSHI, OGINO CHIAKI, KONDO AKIHIKO

  第21回化学工学会学生発表会, Mar. 2019, Japanese, 京都大学桂キャンパス, Domestic conference

  Poster presentation


• ピルビン酸代謝フロー改変を用いた 2,3-ブタンジオール 高生産麹菌の構築

  Zhang Silai, WAKAI SATOSHI, OGINO CHIAKI, 堤 浩子, 秦 洋二, 近藤 昭彦

  日本農芸化学会2019年度大会, Mar. 2019, Japanese, 東京農業大学 世田谷キャンパス, Domestic conference

  Poster presentation


• ナノポアシーケンサを用いた遺伝子組換え麹菌のゲノミ クスおよびトランスクリプトミクス

  WAKAI SATOSHI, Zhang Silai, OGINO CHIAKI, 堤 浩子, 秦 洋二, 近藤 昭彦

  日本農芸化学会2019年度大会, Mar. 2019, Japanese, 東京農業大学 世田谷キャンパス, Domestic conference

  Poster presentation


• PEG化チタンナノ粒子の種々の表面修飾による特性解析

  小寺澤 翔太, 森田 健太, NISHIMURA YUYA, OGINO CHIAKI, KONDO AKIHIKO

  第21回化学工学会学生発表会, Mar. 2019, Japanese, 京都大学桂キャンパス, Domestic conference

  Poster presentation


• Bio-refinery strategy by collaboration Asian's partnership

  Chiaki OGINO, Prihardi Kahar, Akihiko KONDO

  18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), 2019, APCChE, Sapporo Convention Center


• Direct increase of intracellular hydrogen peroxide and enhancement for radiosensitivity with polyacrylic acid-modified titanium peroxide nanoparticles

  Chiaki OGINO, Kenta MORITA, Yuya NISHIMURA, Chiya NUMAKO, Masao NAKAYAMA, Ryohei SASAKI, Akihiko KONDO

  18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), 2019, APCChE, Sapporo Convention Center


• 乳酸生産麹菌のピルビン酸ミトコンドリアキャリアタンパク質欠損における代謝変動

  Zhang Silai, WAKAI SATOSHI, OGINO CHIAKI, 堤 浩子, 秦 洋二, KONDO AKIHIKO

  第１８回糸状菌分子生物学コンファレンス, Nov. 2018, Japanese, シティホールプラザ「アオーレ長岡」, Domestic conference

  Poster presentation


• 外来遺伝子発現用のコムギ細胞外排出シグナルと乾燥誘導性プロモーターの効果

  大野 良子, 久野 裕, 山根 美樹, 佐藤 和広, OGINO CHIAKI, KONDO AKIHIKO, TAKUMI SHIGEO

  第13回ムギ類研究会, Nov. 2018, Japanese, 横浜市立大学木原生物学研究所, Domestic conference

  Poster presentation


• 黄麹菌における汎用性高発現プロモーター

  片山 周平, Zhang Silai, WAKAI SATOSHI, 堤 浩子, 秦 洋二, OGINO CHIAKI, KONDO AKIHIKO

  第１８回糸状菌分子生物学コンファレンス, Nov. 2018, Japanese, シティホールプラザ「アオーレ長岡」, Domestic conference

  Poster presentation


• マルチコピー遺伝子導入黄麹菌のゲノム構造解析

  WAKAI SATOSHI, OGINO CHIAKI, 堤 浩子, 秦 洋二, KONDO AKIHIKO

  第１８回糸状菌分子生物学コンファレンス, Nov. 2018, Japanese, シティホールプラザ「アオーレ長岡」, Domestic conference

  Poster presentation


• バイオマスから基礎化学品原料をつくる(2)

  HIRANO YOSHIAKI, OKUDA NORIMASA, TAKAHASHI TSUKASA, ARIYOSHI KIMIO, OGINO CHIAKI, KITA YUICHI

  異業種交差点Ⅱ, Nov. 2018, Japanese, 新化学技術推進協会, 一橋講堂 2階中会議室, Domestic conference

  Poster presentation


• Target cancer therapy by inorganic nanoparticle

  MORITA Kenta, SUZUKI Takahiro, NISHIMURA Yuya, OGINO Chiaki, KONDO Akihiko

  The 24th Symposium of Young Asian Biological Engineers’ Community (YABEC 2018), Nov. 2018, English, National Taiwan University, International conference

  Oral presentation


• Caffeic acid production from lignocellulosic biomass by metabolically engineered Eshrichaia coli

  KAWAGUCHI Hideo, SAZUKA Takashi, KATSUYAMA Yohei, TERAMOTO Jun, NISHIDA Keiji, MINAMI Hiromichi, OGINO Chiaki, OHNISHI Yasuo, KONDO Akihiko

  The 24th Symposium of Young Asian Biological Engineers’ Community (YABEC 2018), Nov. 2018, English, National Taiwan University, International conference

  Oral presentation


• 油脂酵母Lipomyces starkeyi D35株の 繰り返し発酵による脂肪酸組成変化解析

  MIYAMOTO NAO, PRIHARDI KAHAR, OGINO CHIAKI, KONDO AKIHIKO

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 免疫クリアランスを回避する生体膜由来ドラッグデリバリーキャリアの開発

  AKAMINE RAN, NISHIMURA YUYA, OGINO CHIAKI, KONDO AKIHIKO

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 乳酸高生産性黄麹菌における乳酸生産フラックス強化による乳酸生産性の向上

  Zhang Silai, WAKAI SATOSHI, OGINO CHIAKI, 堤 浩子, 秦 洋二, KONDO AKIHIKO

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 二次代謝系の動的特性解析における大過剰化合物測定の有無の影響の数理解析

  富永 大輔, 川口 秀夫, 堀 良美, 蓮沼 誠久, 荻野 千秋, 油谷 幸代

  第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 組換えコリネ型細菌を用いた3-アミノ-4-ヒドロキシ安息香酸の発酵生産における酸素制限による 促進作用

  KAWAGUCHI HIDEO, 宮崎 翔子, OGINO CHIAKI, 大西 康夫, KONDO AKIHIKO

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 最少量の低毒性イオン液体を用いた前処理に基づく木質系バイオマスリファイナリー

  仁宮 一章, Amaliyah Rohsari, Indah Utami, 柘植 陽太, OGINO CHIAKI, 髙橋 憲司

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 最少量の低毒性イオン液体を用いたバイオマスからバイオ燃料へのHigh-loadingワンポット変換

  杉野 雄規, 柘植 陽太, OGINO CHIAKI, 髙橋 憲司, 仁宮 一章

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 麹菌内で同調的発現挙動を示すプロモーターを用いたタンパク質生産挙動の解析

  片山 周平, Zhang Silai, WAKAI SATOSHI, 堤 浩子, 秦 洋二, OGINO CHIAKI, KONDO AKIHIKO

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 好熱性放線菌Streptomyces thermoviolaceus の培養特性解析

  MIHARA EMI, TAKENAKA MUSASHI, KASHIWAGI NORIMASA, SOTA MASAHIRO, OGINO CHIAKI, KONDO AKIHIKO

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 原子間力顕微鏡を用いたB型肝炎感染機構の解明

  NISHIDA SHINICHIRO, TAKENAKA MUSASHI, NISHIMURA YUYA, OGINO CHIAKI, KONDO AKIHIKO

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 機械学習を用いた酵素のEC number予測

  WATANABE NAOKI, MURATA MASAHIRO, OGINO CHIAKI, KONDO AKIHIKO, ARAKI MICHIHIRO

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• バイオマス分解酵素の大規模スクリーニングで見出した希少放線菌

  TAKENAKA MUSASHI, Lee Jae Min, Pamella Apriliana, KASHIWAGI NORIMASA, PRIHARDI KAHAR, OGINO CHIAKI, KONDO AKIHIKO

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• ナノポアシーケンサを用いた遺伝子組換え麹菌ゲノム上の長鎖タンデムリピート構造の解析

  WAKAI SATOSHI, OGINO CHIAKI, 堤 浩子, 秦 洋二, KONDO AKIHIKO

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• The enhancement of culture conditions for high cell and lipid production of oleaginous yeast Lipomyces starkeyii D35

  Rezky Lastinov Amza, PRIHARDI KAHAR, Ario Betha Juanssilfero, Hiromi Otsuka, Chie Kihira, Chiaki Ogino, Akihiko Kondo

  第70回日本生物工学会大会, Sep. 2018, English, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• Selection of oleaginous yeast capable of high lipid accumulating during challenges from inhibitory chemical complexes

  Ario Betha Juanssilfero, PRIHARDI KAHAR, Rezky Lastinov Amza, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  第70回日本生物工学会大会, Sep. 2018, English, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• Repeated ethanol fermentation from membrane-concentrated sweet sorghum juice by flocculating yeast Saccharomyces cerevisiae

  Hans Wijaya, Kengo Sasaki, Prihardi Kahar, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  第70回日本生物工学会大会, Sep. 2018, English, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• Preparation and characterisation of robust whole cell biocatalyst from Aspergillus oryzae expressing Fusarium heterosporum lipase immobilized onto activated carbon for biodiesel synthesis

  Emmanuel Quayson, AMOAH JEROME, Akihiko Kondo, Shinji Hama, Ayumi Yoshida, Chiaki Ogino

  第70回日本生物工学会大会, Sep. 2018, English, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• Optimization on biodiesel synthesis from palm oil mill effluent (POME) by integrated strategy with lipasecatalyzed ethanolysis

  Nova Rachmadona, AMOAH JEROME, Shinji Hama, Ayumi Yoshida, Akihiko Kondo, Chiaki Ogino

  第70回日本生物工学会大会, Sep. 2018, English, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• mRNAサイレンシングを用いた過酸化チタンナノ粒子の放射線増感機序解明

  OTORI SEISHU, MORITA KENTA, NISHIMURA YUYA, OGINO CHIAKI, KONDO AKIHIKO

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• Improvement of mass mixing by Max-Blend and anchor shape impeller in the high-solid state scarification of kraft pulp

  Deddy Triyono, Nugroho Adi, Chiaki Ogino, Akihiko Kondo

  第70回日本生物工学会大会, Sep. 2018, English, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• Effect of immobilized Chlorella Sorokiniana culture on lipid accumulation and CO2 sequestration

  Daniel Alejandro Alfaro Sayes, AMOAH JEROME, Shinji Hama, Ayumi Yoshida, Akihiko Kondo, Chiaki Ogino

  第70回日本生物工学会大会, Sep. 2018, English, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• Dilute maleic acid pretreatment of sugarcane bagasse and oil palm empty fruit bunch fiber for enhanced enzymatic digestibility

  Lucky Risanto, Deddy Triyono, Nugroho Adi, Triyani, Hiroshi Teramura, Euis Hermiati, Chiaki Ogino, Akihiko Kondo

  第70回日本生物工学会大会, Sep. 2018, English, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 免疫クリアランスの回避を目指した生体膜由来キャリアの開発

  赤嶺 藍, NISHIMURA YUYA, OGINO CHIAKI, KONDO AKIHIKO

  第34回日本DDS学会学術集会, Jun. 2018, Japanese, 長崎ブリックホール, Domestic conference

  Poster presentation


• 新規放射線増感剤としての過酸化チタンナノ粒子の物性解析

  森田 健太, NISHIMURA YUYA, 大取 靖秀, 荒井 優紀, 沼子 千弥, OGINO CHIAKI

  第34回日本DDS学会学術集会, Jun. 2018, Japanese, 長崎ブリックホール, Domestic conference

  Poster presentation


• 過酸化チタンナノ粒子の併用による放射線増感治療法の開発

  NISHIMURA YUYA, 森田 健太, OGINO CHIAKI, 犬伏 祥子, SASAKI RYOHEI, KONDO AKIHIKO

  第34回日本DDS学会学術集会, Jun. 2018, Japanese, 長崎ブリックホール, Domestic conference

  Poster presentation


• バイオマスから基礎化学品原料をつくる

  HIRANO YOSHIAKI, OKUDA NORIMASA, TAKAHASHI TSUKASA, ARIYOSHI KIMIO, OGINO CHIAKI, KITA YUICHI

  第7回JACI/GSCシンポジウム, Jun. 2018, Japanese, 新化学技術推進協会, ANA CROWNE PLAZA KOBE, Domestic conference

  Poster presentation


• Converting Chlamydomonas sp. JSC4 lipids to biodiesel via biocatalysis with Fusarium heterosporum lipase-expressing Aspergillus oryzae whole-cell

  JEROME Amoah, SHIH-HSIN. Ho, SHINJI Hama, AYUMI Yoshida, AKIHITO Nakanishi, TOMOHISA Hasunuma, CHIAKI Ogino, AKIHIKO Kondo

  The 8th International Conference on Algal Biomass, Biofuels and Bioproducts (AlgalBBB 2018), Jun. 2018, English, Motif Seattle, International conference

  Poster presentation


• 多孔体粒子を用いた固定化培養槽の撹拌性能調査

  三原 裕香, Narges Ghobadi, OHMURA NAOTO, OGINO CHIAKI, HORIE TAKAFUMI

  化学工学会，第83年会, Mar. 2018, Japanese, 関西大学, Domestic conference

  Poster presentation


• 酸素をスイッチとしたコリネ型細菌中央代謝経路の切り替え

  小林 俊介, 岩崎 還帰, Hideo Kawaguchi, 仁宮 一章, Chiaki Ogino, 高橋 憲司, Akihiko Kondo, 柘植 陽太

  日本農芸化学会2018年度大会, Mar. 2018, Japanese, 日本農芸化学会, 名古屋市, Domestic conference

  Poster presentation


• 麹菌ミトコンドリア; ピルビン酸キャリアタンパク質欠損株におけるピルビン酸代謝フラックス改変による有用化合物生産

  張 斯来, Satoshi Wakai, Chiaki Ogino, 堤 浩子, 秦 洋二, Akihiko Kondo

  日本農芸化学会2018年度大会, Mar. 2018, Japanese, 日本農芸化学会, 名古屋市, Domestic conference

  Poster presentation


• 酵素改変を指向したin silico解析

  Satoshi Wakai, Chiaki Ogino, Akihiko Kondo

  第3回デザイン生命工学研究会, Mar. 2018, Japanese, デザイン生命工学研究, 沖縄県国頭郡今帰仁村, Domestic conference

  Poster presentation


• リグノセルロース系バイオマスを包括的に利用したコリネ型細菌によるバイオモノマー生産

  Hideo Kawaguchi, Chiaki Ogino, Akihiko Kondo

  日本農芸化学会2018年度大会, Mar. 2018, Japanese, 日本農芸化学会, 名古屋市, Domestic conference

  Poster presentation


• リグノセルロースナノファイバー表面のリグニン分布解析手法の開発

  冨田 康平, Musashi Takenaka, Kenta Morita, Hideo Kawaguchi, Chiaki Ogino, Akihiko Kondo

  日本化学会第98春季年会2018, Mar. 2018, Japanese, 日本化学会, 船橋市, Domestic conference

  Poster presentation


• Saccharomyces cerevisiae酵母におけるCandida boidinii由来キシロース発酵代謝系の導入効果

  PRIHARDI KAHAR, Chie Kihira, Hiromi Otsuka, Chiaki Ogino, Akihiko Kondo

  日本農芸化学会2018年度大会, Mar. 2018, Japanese, 日本農芸化学会, 名古屋市, Domestic conference

  Poster presentation


• 同時糖化発酵プロセスを利用した紙パルプを原料とするカフェ酸の生産

  Hideo Kawaguchi, 勝山陽平, ト タンヤオ, Shoko Tsuruta, Hiromichi Minami, Chiaki Ogino, 大西康夫, Akihiko Kondo

  第13回バイオマス科学会議, Jan. 2018, English, 日本エネルギー学会, 仙台市, International conference

  Oral presentation


• Efficient Xylitol Production from Kraft Pulp by a Cell Surface Engineered Strain ofSaccharomyces cerevisiae

  GUIRIMAND GEORGES, YVES GREGORY, Kentaro Inokuma, Takahiro Bamba, Kengo Sasaki, Chiaki Ogino, Tomohisa Hasunuma, Akihiko Kondo

  5th Asian Conference on Biomass Science, Jan. 2018, English, 日本エネルギー学会, 仙台市, International conference

  Poster presentation


• 麦わらのバイオマス利用に関するパンコムギ系統間差異

  大野 良子, 寺村 浩, OGINO CHIAKI, KONDO AKIHIKO, TAKUMI SHIGEO

  第12回ムギ類研究会, Dec. 2017, Japanese, 京都大学, Domestic conference

  Poster presentation


• 麹菌のミトコンドリア局在ピルビン酸キャリアタンパク質欠損による乳酸生産性の向上

  張 斯来, Satoshi Wakai, Chiaki Ogino, 堤 浩子, 秦 洋二, Akihiko Kondo

  第17回糸状菌分子生物学コンファレンス, Nov. 2017, Japanese, 糸状菌分子生物学研究会, 佐賀市, Domestic conference

  Oral presentation


• 麹菌の co-transformation 時のコピー数多型性に関する解析

  Satoshi Wakai, Chiaki Ogino, 堤 浩子, 秦 洋二, Akihiko Kondo

  第17回糸状菌分子生物学コンファレンス, Nov. 2017, Japanese, 糸状菌分子生物学研究会, 佐賀市, Domestic conference

  Oral presentation


• リグニン残量を制御したセルロースから得られた各種ナノファイバーの構造と物性

  MURAKAMI DAISUKE, MATSUMOTO TAKUYA, OHSHIMA TOMOKO, TERAMURA HIROSHI, KAWAGUCHI HIDEO, OGINO CHIAKI, KONDO AKIHIKO, NISHINO TAKASHI

  第7回CSJ化学フェスタ2017, Oct. 2017, Japanese, 日本化学会, 東京都, Domestic conference

  Poster presentation


• In situ detection of an arming yeast cellulase using AFM

  Chiaki Ogino

  The 23th Symposium of Young Asian Biochemical Engineers' Communyty (YABEC2017), Oct. 2017, English, Xi'an, China, International conference

  [Invited]

  Invited oral presentation


• 発酵阻害物質耐性Candida boidinii K212のキシロース発酵の解析

  PRIHARDI KAHAR, Chie Kihira, Hiromi Otsuka, Chiaki Ogino, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference

  Poster presentation


• 発酵阻害耐性酵母由来強力なENO1プロモーターの発現解析

  Nurlina B. Azmi, PRIHARDI KAHAR, Akiho Itomi, Chie Kihira, Chiaki Ogino, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference

  Poster presentation


• 酸素をスイッチとした二つの樹脂原料の個別生産

  小林 俊介, 柘植 陽太, 岩崎 還帰, KAWAGUCHI HIDEO, 仁宮 一章, Chiaki Ogino, 高橋 憲司, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference

  Poster presentation


• 麹菌による複数遺伝子の同時高発現を可能にするプロモーターセットの開発

  Shuhei Katayama, Satoshi Wakai, 堤 浩子, 秦 洋二, Chiaki Ogino, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference

  Poster presentation


• 高度膜分離技術によるソルガム搾汁液からのスクロース分離および連続的エタノール生産

  Kengo Sasaki, Hideo Kawaguchi, Chiaki Ogino, 佐塚 隆志, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference

  Oral presentation


• The establishment high cell density culture of oleaginous Lipomyces starkeyi D35 for high cell and lipid production

  Rezky Lastinov Amza, PRIHARDI KAHAR, Ario Betha Juanssilfero, Hiromi Otsuka, Chie Kihira, Chiaki Ogino, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference

  Poster presentation


• High Production of single cell oil from glucose and xylose using oleaginous yeast Lipomyces starkeyi

  Ario Betha Juanssilfero, PRIHARDI KAHAR, Rezky, Lastinov Amza, Hiromi Otsuka, Hana Matsumoto, Chie Kihira, Ahmad Thontowi, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference

  Poster presentation


• Exploration of potential indigenous marine actinomycetes from indonesia soil producing enzyme lignocellulosic for biorefinery application

  Apriliana Pamella, Nanik Rahmani, Fahrurrozi Izzuddin, Puspita Lisdiyanti, Jaemin Lee, PRIHARDI KAHAR, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference

  Poster presentation


• Enhanced cell surface engineering of Saccharomyces cerevisiae and optimization of the fermentation process for efficient xylitol production from kraft pulp

  GUIRIMAND GEORGES, YVES GREGORY, Kentaro Inokuma, Takahiro Bamba, Kengo Sasaki, Chiaki Ogino, Tomohisa Hasunuma, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference

  Oral presentation


• B型肝炎ウイルス由来ペプチドを用いたAFMによる肝細胞の受容体検出

  Shinichiro Nishida, Musashi Takenaka, Kenta Morita, Yuya Nishimura, Chiaki Ogino, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference

  Poster presentation


• リグニン量を制御したセルロースナノファイバーの構造と物性

  MURAKAMI DAISUKE, MATSUMOTO TAKUYA, OHSHIMA TOMOKO, TERAMURA HIROSHI, KAWAGUCHI HIDEO, OGINO CHIAKI, KONDO AKIHIKO, NISHINO TAKASHI

  第63回高分子研究発表会(神戸), Jul. 2017, Japanese, 高分子学会関西支部, 神戸市, Domestic conference

  Oral presentation


• リグニン含有量を異にするセルロースから得られた各種ナノファイバーの構造と物性

  MURAKAMI DAISUKE, MATSUMOTO TAKUYA, OHSHIMA TOMOKO, TERAMURA HIROSHI, KAWAGUCHI HIDEO, OGINO CHIAKI, KONDO AKIHIKO, NISHINO TAKASHI

  平成29年度繊維学会年次大会, Jun. 2017, Japanese, 繊維学会, 東京都, Domestic conference

  Poster presentation


• 組換え大腸菌によるグルコースを原料とする4-アミノ 桂皮酸の直接発酵生産

  Kawaguchi Hideo, 若井桂子, 桝尾俊介, 高谷直樹, OGINO Chiaki, KONDO Akihiko

  日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• 肝細胞認識ペプチドを用いた AFMによる肝癌細胞の力学的評価

  Nishida Shinichiro, Suzuki Takahiro, Morita Kenta, 竹中 武蔵, Nishimura Yuuya, OGINO Chiaki, KONDO Akihiko

  第19回化学工学会学生発表会, Mar. 2017, Japanese, 化学工学会関西支部, 豊中市, Domestic conference

  Oral presentation


• 遺伝子組み換え麹菌培養上清カクテルを用いた希少糖高生産プロセスの開発

  Wakai Satoshi, 浅井菜々実, OGINO Chiaki, 堤浩子, 秦洋二, 松沢智彦, 矢追克郎, KONDO Akihiko

  日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• バイオマスの効率的な分解を志向したマルチセルラーゼ高発現麹菌の開発

  片山 周平, Wakai Satoshi, 浅井 菜々実, 堤 浩子, 秦 洋二, OGINO Chiaki, KONDO Akihiko

  第19回化学工学会学生発表会, Mar. 2017, Japanese, 化学工学会関西支部, 豊中市, Domestic conference

  Oral presentation


• デザインされた生体分子の触診デバイス

  竹中 武藏, OGINO Chiaki, KONDO Akihiko

  第２回デザイン生命工学研究会大会, Mar. 2017, Japanese, デザイン生命工学研究会, 神戸市, Domestic conference

  Oral presentation


• コリネ型細菌を用いたフェルラ酸からプロトカテク酸の生産

  岡井直子, 増田敬也, 竹島康誠, 田中耕生, 吉田健一, 宮本正教, OGINO Chiaki, KONDO Akihiko

  日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• REDOXバランスにおける発酵阻害耐性酵母由来新規TDHxの影響

  Azmi Nurlina, 糸見 明穂, PRIHARDI KAHAR, OGINO Chiaki, KONDO Akihiko

  第19回化学工学会学生発表会, Mar. 2017, Japanese, 化学工学会関西支部, 豊中市, Domestic conference

  Oral presentation


• デンプン合成制御因子CRCTが稲わらを用いたバイオエタノール生産に及ぼす効果

  森田 隆太郎, 寺村 浩, OGINO CHIAKI, KONDO AKIHIKO, FUKAYAMA HIROSHI

  日本作物学会講演会, 2017, Japanese, Domestic conference

  Poster presentation


• 代謝改変およびフラックス強化した黄麹菌でのデンプンからの乳酸生産

  Sasakura Naoya, Wakai Satoshi, 浅井菜々実, OGINO Chiaki, 堤 浩子, 秦 洋二, KONDO Akihiko

  第16回糸状菌分子生物学コンファレンス, Nov. 2016, Japanese, 糸状菌分子生物学研究会, 宇治市, Domestic conference

  Oral presentation


• 麹菌におけるゲノムを切らずに書き換える新規ゲノム編集ツールの確立

  荒添 貴之, 西田 敬二, 田畑 麻由良, 若井 暁, 荻野 千秋, 秦 洋二, 近藤 昭彦

  第16回糸状菌分子生物学カンファレンス, Nov. 2016, Japanese, 宇治おうばくプラザ, Domestic conference

  Poster presentation


• 麹菌におけるゲノムを切らずに書き換える新規ゲノム編集ツールの確立

  荒添貴之, 西田敬二, 田畑麻由良, Wakai Satoshi, OGINO Chiaki, 秦 洋二, KONDO Akihiko

  第16回糸状菌分子生物学コンファレンス, Nov. 2016, Japanese, 糸状菌分子生物学研究会, 宇治市, Domestic conference

  Oral presentation


• 黄麹菌 Aspergillus oryzae による機能性オリゴ糖の生産

  若井 暁, 浅井菜々実, OGINO Chiaki, 堤 浩子, 秦 洋二, KONDO Akihiko

  第16回糸状菌分子生物学コンファレンス, Nov. 2016, Japanese, 糸状菌分子生物学研究会, 宇治市, Domestic conference

  Oral presentation


• マルチセルラーゼ発現黄麹菌の培養条件によるセルラーゼ生産性の違い

  片山周平, Wakai Satoshi, 浅井菜々実, OGINO Chiaki, 堤 浩子, 秦 洋二, KONDO Akihiko

  第16回糸状菌分子生物学コンファレンス, Nov. 2016, Japanese, 糸状菌分子生物学研究会, 宇治市, Domestic conference

  Oral presentation


• 疎水性アルコールを用いた植物バイオマスの効率的な成分分離法の開発

  寺村浩, Sasaki Kengo, 白井智量, Kawaguchi Hideo, OGINO Chiaki, 菊地淳, 佐塚隆志, KONDO Akihiko

  第61回リグニン討論会, Oct. 2016, Japanese, リグニン討論会実行委員会, 宇治市, Domestic conference

  Oral presentation


• Mapping of endoglucanases displayed on a surface of yeast cell catalyst using atomic force microscopy

  OGINO CHIAKI

  The 22th Symposium of Young Asian Biochemical Engineers' Communyty, Oct. 2016, English, 生物工学会, 宮崎市, International conference

  Oral presentation


• 転写因子改変放線菌を用いた異種タンパク質生産

  柏木 紀賢, 西岡 雅都, 廣瀬 修一, 曽田 匡洋, OGINO Chiaki, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• 担癌マウスを用いたポリアクリル酸修飾過酸化チタンナノ粒子の生体内分布の解明

  Suzuki Takahiro, Morita Kenta, Nishimura Yuuya, OGINO Chiaki, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• 新規放射線増感治療に向けた過酸化チタンナノ粒子のマウス生体内分布特性の調査

  Suzuki Takahiro, Morita Kenta, Nishimura Yuuya, OGINO Chiaki, KONDO Akihiko

  化学工学会第48回秋季大会, Sep. 2016, Japanese, 化学工学会, 徳島市, Domestic conference

  Oral presentation


• 酸化チタン類ナノ粒子と物理的外部刺激を併用した新規がん治療の提案

  OGINO Chiaki, Morita Kenta, Suzuki Takahiro, Nishimura Yuuya, 中山雅央, 佐々 木良平, KONDO Akihiko, 沼子千弥

  セラミック協会第 29 回秋季シンポジウム, Sep. 2016, Japanese, セラミック協会, 東広島市, Domestic conference

  Oral presentation


• 三種セルラーゼ共・強発現麹菌によるセルロースからのコウジ酸生産

  Wakai Satoshi, 浅井 菜々実, OGINO Chiaki, 堤 浩子, 秦 洋二, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• 原料となる植物バイオマスの種類がセルロー スナノファイバーの強度に与える影響の解析

  寺村 浩, 延田 紘治, OGINO Chiaki, 北野 英己, 平野 恒, 佐塚 隆志, 西野 孝, KONDO Akihiko

  第34回日本植物細胞分子生物学会（上田）大会, Sep. 2016, Japanese, 日本植物細胞分子生物学会, 長野県上田市, Domestic conference

  Oral presentation


• 凝集性酵母における新規な阻害剤耐性機構の解明

  糸見 明穂, 紀平 和枝, Kahar Prihardi, OGINO Chiaki, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• 遺伝子組換え大腸菌を用いたフェニル乳酸生産に影響を与える遺伝子の発現解析

  宮川 寛規, Kawaguchi Hideo, OGINO Chiaki, 老沼 研一, 高谷 直樹, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• σ因子欠損放線菌の3,4-AHBA生産への影響

  釜我 圭, 柏木 紀賢, OGINO Chiaki, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• バイオナノカプセルとリポソームの複合粒子によるin vivoでの抗腫瘍効果

  Nishimura Yuuya, Suzuki Takahiro, Morita Kenta, OGINO Chiaki, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• ナノ粒子とX線照射の併用による低侵襲がん治療法の開発

  Morita Kenta, Nishimura Yuuya, Suzuki Takahiro, OGINO Chiaki, KONDO Akihiko

  化学工学会第48回秋季大会, Sep. 2016, Japanese, 化学工学会, 徳島市, Domestic conference

  Oral presentation


• コリスミ酸ピルビン酸リアーゼ発現コリネ型細菌を用いたプロトカテク酸の生産

  岡井 直子, 三好 孝則, 竹嶋 康誠, 田中 耕生, 吉田 健一, 桑原 広明, OGINO Chiaki, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• アルコールの添加が希硫酸前処理の効率に与える影響の解析

  OGINO Chiaki, 寺村 浩, SASAKI KENGO, Kawaguchi Hideo, 佐塚 隆志, KONDO Akihiko

  化学工学会第48回秋季大会, Sep. 2016, Japanese, 化学工学会, 徳島市, Domestic conference

  Oral presentation


• 膵臓がん治療に向けた新規放射線増感剤としての過酸化チタンナノ粒子のin vitro評価

  Morita Kenta, Suzuki Takahiro, Nishimura Yuuya, 松本和久, 沼子千弥, 佐藤和好, 中山雅央, 佐々木良平, OGINO Chiaki, KONDO Akihiko

  生物工学若手研究者の集い夏のセミナー2016, Jul. 2016, Japanese, 生物工学会, 府中市, Domestic conference

  Poster presentation


• 担癌マウスを用いたポリアクリル酸修飾過酸化チタンナノ粒子の生体内分布の解明

  Suzuki Takahiro, Morita Kenta, Nishimura Yuuya, OGINO Chiaki, KONDO Akihiko

  生物工学若手研究者の集い夏のセミナー2016, Jul. 2016, Japanese, 生物工学会, 府中市, Domestic conference

  Poster presentation


• HER2受容体発現細胞を標的としたDDSキャリアの抗腫瘍効果の評価

  Nishimura Yuuya, Suzuki Takahiro, Morita Kenta, OGINO Chiaki, KONDO Akihiko

  生物工学若手研究者の集い夏のセミナー2016, Jul. 2016, Japanese, 生物工学会, 府中市, Domestic conference

  Poster presentation


• AFMで診る・識る・釣る ～核酸・タンパク・細胞の研究～

  TAKENAKA Musashi, OGINO Chiaki, KONDO Akihiko

  生物工学若手研究者の集い夏のセミナー2016, Jul. 2016, Japanese, 生物工学会, 府中市, Domestic conference

  Poster presentation


• Mechanical Properties of Lignocellulose Nanofiber Sheets Prerared in Butanol / Sulfuric Acid Method

  MATSUMOTO TAKUYA, KOBAYASHI(OKAHISA) YOKO, TERAMURA HIROSHI, OSHIMA TOMOKO, MORITA KENTA, HONGO CHIZURU, KAWAGUCHI HIDEO, OGINO CHIAKI, KONDO AKIHIKO, NISHINO TAKASHI

  第5回 JACI/GSCシンポジウム, Jun. 2016, Japanese, 公益社団法人 新化学技術推進協会, 神戸市, Domestic conference

  Poster presentation


• アルコールの添加が希硫酸前処理の効率に与える影響の解析

  Teramura Hiroshi, KAWAGUCHI Hideo, Sazuka Takashi, OGINO Chiaki, KONDO Akihiko

  第5回JACI GSCシンポジウム, Jun. 2016, Japanese, 新化学技術推進協会, 神戸市, Domestic conference

  Poster presentation


• Optimization of Displayed Cellulases in Yeast Suitable for Ionic Liquid Pretreated Biomass by Cocktail δ Integration Methodology

  OGINO Chiaki, Naoya Ishizue, KONDO Akihiko

  Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conference

  Oral presentation


• Engineering and Analyzing Streptomyces strain for the Recombinant Protein Production

  Norimasa Kashiwagi, Satoko Niimi-Nakamura, Hirose Syuuichi, Masahiro Sota, OGINO Chiaki, KONDO Akihiko

  Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conference

  Oral presentation


• Direct Fermentation of L-Lactic Acid from Starch By Genetically Engineered Aspergillus Oryzae

  Wakai Satoshi, Sasakura Naoya, Nanami Asai-Nakashima, Hiroko Tsutumi, Yoji Hata, OGINO Chiaki, KONDO Akihiko

  Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conference

  Oral presentation


• Development of Platform Yeast Strain Capable of Direct Fermentation of Raw Biomass to Ethanol

  PRIHARDI KAHAR, Akiho Itomi, Ahmad Thontowi, Hiromi Otsuka, Chie Kihira, Jaemin Lee, Ario Betha Juanssil fero, Apridah Cameliawati Djohan, Yopi Sunarya, Ishii Jun, OGINO Chiaki, Banbang Prasetya, KONDO Akihiko

  Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conference

  Poster presentation


• Caffeic Acid Production By Simultaneous Saccharification and Fermentation of Kraft Pulp Using Recombinant Escherichia coli

  Kawaguchi Hideo, Katsuyama Youhei, Du Danyao, Sachiko Nakamura-Tsuruta, Hiroshi teramura, Hiromichi Minami, OGINO Chiaki, Yasuo Ohnishi, KONDO Akihiko

  Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conference

  Oral presentation


• 担癌マウスを用いたポリアクリル酸修飾過酸化チタンナノ粒子の生体内分布特性の調査

  Suzuki Takahiro, Morita Kenta, Nishimura Yuuya, Ogino Chiaki, Kondo Akihiko

  化学工学会第81年会, Mar. 2016, Japanese, Domestic conference

  Oral presentation


• 紙パルプの同時糖化発酵によるカフェ酸の微生物生産

  Kawaguchi Hideo, Katsuyama Youhei, トタンヤオ, Tsuruta Syoko, Minami Hiromichi, Ogino Chiaki, Oonishi Yasuo, Kondo Akihiko

  日本農芸化学会2016年度大会, Mar. 2016, Japanese, Domestic conference

  Oral presentation


• マンナンバイオマスからのエタノール生産：βｰマンナナーゼとβｰマンノシダーゼを細胞表層に提示した出芽酵母の開発

  ISHII JUN, OKAZAKI FUMIYOSHI, Apridah Cameliawati Djohan, HARA KIYOTAKA, ASAI NANAMI, Ade Andriani, TERAMURA HIROSHI, Prihardi Kahar, YOPI, Bambang Prasetya, OGINO CHIAKI, AKIHIKO KONDO

  日本農芸化学会2016年度大会, Mar. 2016, Japanese, Domestic conference

  Oral presentation


• コリネ型細菌によるUV吸収性アミノ酸の生産

  Tsuge Youta, Yamamoto Shogo, Masuda Masahiro, Ogino Chiaki, Kondo Akihiko

  日本農芸化学会2016年度大会, Mar. 2016, Japanese, Domestic conference

  Oral presentation


• アルコールの添加物が希硫酸前処理の効率に与える影響の解析

  Teramura Hiroshi, Sasaki Kengo, Shirai Tomokazu, Kawaguchi Hideo, Ogino Chiaki, Kikuchi Atushi, Sazuka Takashi, Kondo Akihiko

  日本農芸化学会2016年度大会, Mar. 2016, Japanese, Domestic conference

  Oral presentation


• From mannan and lignocellulosic biomass to biochemicals: cell surface display and metabolic engineering in yeast Saccharomyces cerevisiae

  ISHII JUN, OKAZAKI FUMIYOSHI, Shuhei Ishikado, Apridah Cameliawati Djohan, Nanami Asai-Nakashima, Yopi, Fumio Matsuda, OGINO CHIAKI, AKIHIKO KONDO

  The 6th iBioK Asian Workshop, Mar. 2016, Japanese, Kobe, International conference

  Oral presentation


• AFM力計測を用いた細胞表層提示酵素の特異的検出とマッピング評価

  Ogino Chiaki, Takenaka Musashi, Inokuma Kentarou, Hasunuma Tomohisa, Kondo Akihiko

  化学工学会第81年会, Mar. 2016, Japanese, Domestic conference

  Oral presentation


• AFM力学計測法を利用した細胞表層空間に提示した酵素検出

  Takenaka Musashi, Ogino Chiaki, Kondo Akihiko, Inokuma Kentarou, Hasunuma Tomohisa, Kobayashi Takuya

  日本化学会第96春季年回, Mar. 2016, Japanese, Domestic conference

  Oral presentation


• Affibody提示バイオナノカプセル/リポソーム融合粒子を用いたin vivo での抗腫瘍効果

  Nishimura Yuuya, Suzuki Takahiro, Morita Kenta, Ogino Chiaki, Kondou Akihiko

  化学工学会第81年会, Mar. 2016, Japanese, Domestic conference

  Oral presentation


• リグノセルロース系バイオマスの同時糖化発酵によるフェニル乳酸生産

  Kawaguchi hideo, Teramura Hiroshi, Nakamura Satoko, Ogino Chiaki, Hara Kiyotaka, Hasunuma Tomohisa, Rounuma Kenichi, Takatani Naoki, Hirano Wataru, Sazuka Takashi, Kitano Hideki, Kondo Akihiko

  第11回バイオマス科学会議, Jan. 2016, Japanese, International conference

  Oral presentation


• 稲わらのバイオリファイナリーに向けた特性の解析と多様性の評価

  GOUDA TAKASHI, TERANUMA HIROSHI, SUEHIRO MIKI, KANAMARU KENGO, KAWAGUCHI HIDEO, OGINO CHIAKI, KONDO AKIHIKO, YAMAZAKI MASANORI

  神戸大学若手フロンティア2015, Dec. 2015, Japanese, 神戸大学, Domestic conference

  Poster presentation


• バイオリファイナリー利用に向けた稲わらの希硫酸前処理後グルコース含量の自然変異

  GODA TAKASHI, TERAMURA HIROSHI, SUEHIRO MIKI, KANAMARU KENGO, KAWAGUCHI HIDEO, OGINO CHIAKI, KONDO AKIHIKO, YAMAZAKI MASANORI

  日本農芸化学会関西支部第492例会, Dec. 2015, Japanese, 神戸大学, Domestic conference

  Oral presentation


• Specific drug delivery for target cancer tumor using affibody-displaying bionanocapsule/liposome complex

  Yuya Nishimura, Jun Ishii, Chiaki Ogino, Akihiko Kondo

  The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015), Dec. 2015, English, International conference

  Oral presentation


• Live cell imaging and membrane protein mapping with atomic force microscope

  Musashi Takenaka, T. Kobayashi, Yusuke Miyachi, Kentarou Inokuma, Jun Ishii, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015), Dec. 2015, English, International conference

  Oral presentation


• Development of novel radiosensitizing cancer therapy:Combination of radio-therapy and titanium peroxide nanoparticle

  Morita Kenta, Suzuki Takahiro, Nakayama M, Sasaki r, Sato K, Numako Chiya, Ogino Chiaki, Kondo Akihiko

  Pacifichem 2015, Dec. 2015, English, International conference

  Oral presentation


• Characterization for metal-oxide nano-perticles generating radicals with X-ray irrdiatio

  Numako Chiya, Miyazaki S, Sato K, Takami S, Ogino Chiaki, Kondo Akihiko

  Pacifichem 2015, Dec. 2015, English, International conference

  Oral presentation


• コピー数多形とプロモーター強度を加味した黄麹菌でのマルチ遺伝子発現

  Wakai, Asai Nanami, Ogino Chiaki, Tsutsumi Hiroko, Hata Youji, Kondo Akihiko

  第15回糸状菌分子生物学コンファレンス, Nov. 2015, Japanese, Domestic conference

  Oral presentation


• L-乳酸生産黄麹菌の乳酸生産能に対する中央代謝経路改変の影響

  Sasakura Naoya, Wakai, Asai Nanami, Ogino Chiaki, Tsutsumi Hiroko, Hata Youji, Kondo Akihiko

  第15回糸状菌分子生物学コンファレンス, Nov. 2015, Japanese, Domestic conference

  Oral presentation


• 代謝改変による赤色酵母を用いたアスタキサンチン生産性の向上

  Yamamoto Keisuke, Hara Kiyotaka, Ogino Chiaki, Kondou Akihiko

  第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, Japanese, Domestic conference

  Oral presentation


• Streptoverticillium Cinnamoneum由来Phospholipase D プロモーター遺伝子発現機構の調査

  柏木 紀賢, Nisioka Masato, Matsumoto Hana, Hirose Syuuichi, Masuda Masahiro, Ogino Chiaki, Kondou Akihiko

  第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, Japanese, Domestic conference

  Oral presentation


• L-乳酸生産株Aspergillus oryzaeの代謝改変によるLー乳酸生産能の改変

  Sasakura Naoya, Wakai, Asai Nanami, Ogino Chiaki, Tsutsumi Hiroko, Hata Youji, Kondou Akihiko

  第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, Japanese, Domestic conference

  Oral presentation


• Lignocellulosic biomass refinery using biocompatible ionic liquida

  OGINO CHIAKI, NINOMIYA KAZUHIRO, KAKUCHI RYOHEI, ENDO TAKATSUGU, KURODA KOSUKE, NOGUCHI MANA, SHIMIZU NOBUAKI, TAKAHASHI KENJI

  The 21th Symposium of Young Asian Biochemical Engineer's Communyty, Oct. 2015, English, International conference

  Oral presentation


• Conversion of high phospholipid-containing oils to biodiesel using immobilizes Aspergillus oryzae whole cell biocatalyst ezpressing Fusarium heteroporum lipase

  Jerom Amoah, shin-Hsin Ho, Hama Shinji, Yoshida Ayumi, Nakanishi Akihito, Hasunuma Tomohisa, Ogino Chiaki, Kondo Akihiko

  The 21th Symposium of Yong Asian Biochemical engineer's Communyty, Oct. 2015, English, International conference

  Oral presentation


• 放射線治療と相乗効果を有するポリアクリル酸修飾過酸化チタンナノ粒子の作用機序解明

  Morita Kenta, Suzuki Takahiro, Nishimura Yuuya, Numako Chiya, Satou Kazuyoshi, Nakayama Masao, Sasaki Ryouhei, Ogino chiaki, Kondou Akihiko

  化学工学会第47回秋季大会, Sep. 2015, Japanese, Domestic conference

  Oral presentation


• kinetic analysis of the lignin degradation under ultrasonic irradiation

  TOBA Yurina, HORIE Takafumi, TANAKA Koki, OGINO Chiaki, OHMURA Naoto

  SCEJ 47th Autumn Meeting, Sep. 2015, Japanese, The Society of Chemical Engineers, Japan, Hokkaido University, Domestic conference

  Oral presentation


• 担癌マウスを用いたポリアクリル酸修飾過酸化チタンナノ粒子の生体内分布特性の調査

  Suzuki Takahiro, Morita Kenta, Nishimura Yuya, Ogino Chiaki, Kondo Akihiko

  化学工学会第47回秋季大会, Sep. 2015, Japanese, Domestic conference

  Oral presentation


• バイオマスからの微生物によるバイオモノマー生産

  OGINO CHIAKI

  マテリアルサイエンス研究科セミナー, Jul. 2015, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• Screening of ethanol and lactic acid-producing yeast from Indonesia culture collection

  Ahamad Thontowi, Senlie Octaviana, Atit Kanti, Ario Betha Juanssifero, Yopi, Bambang Prasetya, Prihardi Kahar, Ogino Chiaki, Kondo Akihiko

  6th AFOB Regional Symposium, May 2015, English, Asian Federation of Biotechnology, International conference

  Oral presentation


• Screening and characterization of Lignocellulose-hydrolytic from Indonesia Rare Actinomycetes and applications of these enzymes for sugar cane baggase saccharification to produce bioethanol

  Nanik Rahmani, Pamela Apriliana, Alifah M. Jannah, Shanti Ratnakomala, Puspita Lisdiyanti, Yopi, Bambang Prasetya, Jaemin Lee, Prihardi Kahar, Ogino Chiaki, Kondo Akihiko

  6th AFOB Regional Symposium, May 2015, English, Asian Federation of Biotechnology, International conference

  Oral presentation


• 膜濃縮したソルガム搾汁液からのエタノール発酵

  SASAKI KENGO, TSUGE YOTA, SASAKI DAISUKE, 寺村 浩, KAWAGUCHI HIDEO, OGINO CHIAKI, 春日 重光, 佐塚 隆志, KONDO AKIHIKO

  日本農芸化学会2015年度大会, Mar. 2015, Japanese, 日本農芸化学会, 岡山市, Domestic conference

  Oral presentation


• 担癌マウスへのTiナノ粒子投与による生体内分布

  鈴木 貴弘, 田野 亜都里, 森田 健太, OGINO CHIAKI, KONDO AKIHIKO

  第17回化学工学会学生発表会, Mar. 2015, Japanese, 化学工学会, 徳島市, Domestic conference

  Oral presentation


• 組換えコリネ型細菌による3-hydroxy-4-aminobenzoic acid発酵におけるソルガム搾汁液の促進作用

  KAWAGUCHI HIDEO, SASAKI KENGO, 寺村 浩, TSUGE YOTA, 中村 聡子, 菅井 佳宜, 勝山 洋平, 大西 康夫, 平野 恒, 佐塚 隆志, 北野 英巳, 春日 重光, OGINO CHIAKI, KONDO AKIHIKO

  日本農芸化学会2015年度大会, Mar. 2015, Japanese, 日本農芸化学会, 岡山市, Domestic conference

  Oral presentation


• 実バイオマスを微生物変換するための新しい酵母プラットフォームの探索

  PRIHARDI KAHAR, 李 載みん, OGINO CHIAKI, KONDO AKIHIKO

  日本農芸化学会2015年度大会, Mar. 2015, Japanese, 日本農芸化学会, 岡山市, Domestic conference

  Oral presentation


• コリネ型細菌を用いたプロトカテク酸の生産

  OKAI NAOKO, 竹嶋 康誠, OGINO CHIAKI, KONDO AKIHIKO

  日本農芸化学会2015年度大会, Mar. 2015, Japanese, 日本農芸化学会, 岡山市, Domestic conference

  Oral presentation


• AFM による細胞表層提示タンパク質の特異的検出およびマッピング

  竹中 武藏, 小林 拓也, OGINO CHIAKI, KONDO AKIHIKO

  日本化学会第95春季年会, Mar. 2015, Japanese, 日本化学会, 船橋市, Domestic conference

  Oral presentation


• Construction of arming yeast displaying optimal ratio of cellulases for degradation of biomass pretreated with ionic liquid

  OGINO Chiaki, ISHIZUE Naoya, YASUDA Misa, ISHIZAKI Miki, NINOMIYA Kazunori, KONDO Akihiko

  Active Enzyme Molecule 2014, Dec. 2014, English, 酵素工学会, 富山市, Domestic conference

  Poster presentation


• 麹菌細胞工場を用いた L- および D-乳酸の生産

  笹倉 直也, WAKAI SATOSHI, 浅井 菜々実, OGINO CHIAKI, 堤 浩子, 秦 洋二, KONDO AKIHIKO

  第14回糸状菌分子生物学コンファレンス, Nov. 2014, Japanese, 糸状菌分子生物学研究会, 仙台市, Domestic conference

  Poster presentation


• 好熱性および好冷性酵素のドメインを持つキメラ酵素の構築

  WAKAI Satoshi, ASAI Nanami, OGINO Chiaki, TSUTSUMI Hiroko, HATA Yoji, KONDO Akihiko

  第15回極限環境生物学会年会, Nov. 2014, Japanese, 極限環境生物学会, 沖縄市, Domestic conference

  Poster presentation


• セルラーゼ遺伝子をマルチコピー導入した麹菌の遺伝子コピー数とセルラーゼ活性

  WAKAI SATOSHI, 浅井 菜々実, OGINO CHIAKI, 堤 浩子, 秦 洋二, KONDO AKIHIKO

  第14回糸状菌分子生物学コンファレンス, Nov. 2014, Japanese, 糸状菌分子生物学研究会, 仙台市, Domestic conference

  Poster presentation


• Microbial screening for biorefinery research

  OGINO Chiaki

  The 20th Symposium of Young Asian Biochemical Engineers' Communyty, Nov. 2014, English, 生物工学会, Taiwan, China, International conference

  Oral presentation


• イオン液体前処理後バガスに適したアーミング酵母の開発

  石居 直也, 石崎 美紀, 安田 美砂, 仁宮 一章, 高橋 憲司, OGINO CHIAKI, KONDO AKIHIKO

  第5回イオン液体討論会, Oct. 2014, Japanese, イオン液体研究会, 横浜市, Domestic conference

  Oral presentation


• 放線菌を用いた糖を原料とするパラアミノ安息香酸の生産

  OKAI NAOKO, 佐藤 嘉弘, 大野 摩耶, 竹嶋 康誠, 増田 敬哉, 宮本 正教, 樋田 幸三, OGINO CHIAKI, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• 麹菌を用いたデンプンからのD- 乳酸の同時糖化発酵

  WAKAI SATOSHI, 浅井 菜々実, OGINO CHIAKI, 堤 浩子, 秦 洋二, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• 稲わら前処理液の膜プロセスによるエタノール発酵高効率化

  SASAKI KENGO, TSUGE YOTA, SASAKI DAISUKE, OGINO CHIAKI, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• バイオマス糖化液由来成分が大腸菌のフェニル乳酸発酵に与える影響

  KAWAGUCHI HIDEO, 寺村 浩, 中村 聡子, OGINO CHIAKI, HARA KIYOTAKA, HASUNUMA TOMOHISA, 老沼 研一, 高谷 直樹, 平野 恒, 佐塚 隆志, 北野 英己, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• pH 応答性ペプチドGALA を表層提示したバイオナノカプセルのエンドソーム脱出

  西村 勇哉, 江澤 僚将, ISHII JUN, OGINO CHIAKI, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• ｐH応答性膜融合ペプチドGALAを表層提示したバイオナノカプセルのエンドソーム脱出

  西村 勇哉, 竹田 光一, 江澤 僚将, ISHII JUN, OGINO CHIAKI, KONDO AKIHIKO

  生物工学若手研究者の集い夏のセミナー, Jul. 2014, Japanese, 生物工学会, 神戸市, Domestic conference

  Poster presentation


• 放射線がん治療との併用に向けたか酸化チタンナノ粒子の物性評価

  森田 健太, 田野 亜都里, 鈴木 貴弘, 沼子 千弥, 宮嵜 世里加, 佐藤 和好, 中山 雅央, 佐々木 良平, OGINO CHIAKI, KONDO AKIHIKO

  生物工学若手研究者の集い夏のセミナー, Jul. 2014, Japanese, 生物工学会, 神戸市, Domestic conference

  Poster presentation


• 上皮成長因子受容体を特異的に認識するAffibody提示バイオナノカプセルの開発

  江澤 僚将, 西村 勇哉, ISHII JUN, OGINO CHIAKI, KONDO AKIHIKO

  生物工学若手研究者の集い夏のセミナー, Jul. 2014, Japanese, 生物工学会, 神戸市, Domestic conference

  Poster presentation


• リグノセルロース系バイオマスからのバイオ芳香族化合物の生産

  KAWAGUCHI HIDEO, 寺村 浩, 中村 聡子, OGINO CHIAKI, KONDO AKIHIKO

  生物工学若手研究者の集い夏のセミナー, Jul. 2014, Japanese, 生物工学会, 神戸市, Domestic conference

  Poster presentation


• カーボンナノチューブの表面改質を目指した重合性界面活性剤の開発

  舟崎 裕一, 本庄 崇文, 竹中 武蔵, OGINO CHIAKI, Maruyama Tatsuo

  第60回高分子研究発表会（神戸）, Jul. 2014, Japanese, 高分子学会, 神戸市, Domestic conference

  Poster presentation


• AFMを用いた表層提示酵母へのアプローチ及び表層提示酵素の検出

  竹中 武蔵, 小林 拓矢, OGINO CHIAKI, KONDO AKIHIKO

  生物工学若手研究者の集い夏のセミナー, Jul. 2014, Japanese, 生物工学会, 神戸市, Domestic conference

  Poster presentation


• カーボンナノチューブの可溶化を目指した重合性界面活性剤の開発

  舟崎 裕一, 本庄 崇文, 竹中 武蔵, OGINO CHIAKI, Maruyama Tatsuo

  第63回高分子学会年次大会, May 2014, Japanese, 高分子学会, 名古屋市, Domestic conference

  Poster presentation


• 放線菌による 3,4-AHBA 合成とキシロース代謝

  NIIMI-NAKAMURA Satoko, OGINO Chiaki, SUGAI Yoshinori, KATSUYAMA Yohei, ONISHI Yasuo, KONDO Akihiko

  日本農芸化学会2014年度大会, Mar. 2014, Japanese, 東京, Domestic conference

  Oral presentation


• 同時糖化発酵による希硫酸前処理バイオマスからのフェニル乳酸生産

  KAWAGUCHI HIDEO, 寺村 浩, 中村 聡子, OGINO CHIAKI, 老沼 研一, 高谷 直樹, 平 野 恒, 佐塚 隆志, 北野 英己, KONDO AKIHIKO

  日本農芸化学会2014年度大会, Mar. 2014, Japanese, 日本農芸化学会, 川崎市, Domestic conference

  Oral presentation


• 新しい放射線治療をめざした過酸化チタンナノ粒子の物性研究

  森田 健太, 田野 亜都里, 沼子 千弥, 佐藤 和好, 中山 雅央, 佐々木 良平, OGINO CHIAKI, KONDO AKIHIKO

  化学工学会第79年会, Mar. 2014, Japanese, 化学工学会, 岐阜市, Domestic conference

  Poster presentation


• バイオマス評価法の開発

  TERAMUA Hiroshi, YAMASAKI Masanori, KAWAGUCHI Hideo, GODA Takashi, MATSUDA Fumio, OGINO Chiaki, KONDO Akihiko

  第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山, Domestic conference

  Poster presentation


• タンパク質提示酵母の表層における親和力解析

  小林 拓矢, OGINO CHIAKI, KONDO AKIHIKO

  第16回化学工学会学生発表会(堺大会), Mar. 2014, Japanese, 化学工学会, 大阪市, Domestic conference

  Oral presentation


• タンパク高生産放線菌のゲノム変異解析と因子探索

  中村 聡子, 廣瀬 修一, 西岡 雅都, 柏木 紀賢, 曽田 匡洋, OGINO CHIAKI, KONDO AKIHIKO

  第8回日本ゲノム微生物学会年会, Mar. 2014, Japanese, 日本ゲノム微生物学会, 世田谷区, Domestic conference

  Poster presentation


• セルロース系バイオマスからの物質生産を可能にしたスーパー麹菌の開発

  WAKAI SATOSHI, 浅井 菜々実, 山田 亮祐, OGINO CHIAKI, 堤 浩子, 秦 洋二, KONDO AKIHIKO

  日本農芸化学会2014年度大会, Mar. 2014, Japanese, 日本農芸化学会, 川崎市, Domestic conference

  Oral presentation


• コリネ型細菌を用いた糖を原料とするGABAの生産

  OKAI NAOKO, 高橋 千尋, 畑田 一樹, OGINO CHIAKI, KONDO AKIHIKO

  日本農芸化学会2014年度大会, Mar. 2014, Japanese, 日本農芸化学会, 川崎市, Domestic conference

  Oral presentation


• イオン液体を用いたリグノセルロースのトータルリファイナリー

  仁宮 一章, OGINO CHIAKI, 清水 宣明, 高橋 憲司

  化学工学会第79年会, Mar. 2014, Japanese, 化学工学会, 岐阜市, Domestic conference

  Oral presentation


• イオン液体と酸を用いた新規バイオマス前処理法の開発と応用

  小倉 一真, OGINO CHIAKI, KONDO AKIHIKO

  化学工学会第79年会, Mar. 2014, Japanese, 化学工学会, 岐阜市, Domestic conference

  Poster presentation


• 低細胞毒性イオン液体により前処理した木質系バイオマスからの同時糖化発酵

  表 小百合, 仁宮 一章, OGINO CHIAKI, 高橋 憲司, 清水 宣明

  第4回イオン液体研究会, Nov. 2013, Japanese, イオン液体研究会, 横浜市, Domestic conference

  Poster presentation


• 放線菌による芳香族化合物ホモゲンチジン酸の生産

  中村 聡子, KAWAGUCHI HIDEO, OGINO CHIAKI, KONDO AKIHIKO

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• 同時糖化発酵によるセルロースからのフェニル乳酸生産

  KAWAGUCHI HIDEO, 中村 聡子, OGINO CHIAKI, 老沼 研一, 高谷 直樹, KONDO AKIHIKO

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• 低細胞毒性イオン液体により前処理した木質系バイオマスからの同時糖化発酵

  表 小百合, 仁宮 一章, 曽田 裕司, OGINO CHIAKI, 高橋 憲司, 清水 宣明

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• 低コストイオン液体によるエタノール製造プロセスの提案

  齋藤 聡志, 石田 亘広, 神谷 典穂, OGINO CHIAKI

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• 新しい重合性界面活性剤を用いた難溶性ナノ材料可溶化技術の開発

  舟崎 裕一, 本庄 崇文, 宝得 一貴, MINAMI HIDETO, 竹中 武蔵, OGINO CHIAKI, Maruyama Tatsuo

  第62回高分子討論会, Sep. 2013, Japanese, 高分子学会, 金沢市, Domestic conference

  Oral presentation


• 上皮成長因子受容体を特異的に認識するAffibody提示バイオナノカプセルの開発

  江澤 僚将, 西村 勇哉, ISHII JUN, OGINO CHIAKI, KONDO AKIHIKO

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• 酸触媒イオン液体前処理を用いた新規バイオプロセス構築

  小倉 一真, OGINO CHIAKI, KONDO AKIHIKO

  日本農芸化学会関西;中四国;西日本支部合同大会（第481回講演会）, Sep. 2013, Japanese, 日本農芸化学会, 広島市, Domestic conference

  Oral presentation


• 麹菌Aspergillus oryzae によるL-乳酸の生産

  WAKAI SATOSHI, 吉栄 俊秀, 浅井 菜々実, 山田 亮祐, OGINO CHIAKI, 堤 浩子, 秦 洋二, KONDO AKIHIKO

  日本農芸化学会関西;中四国;西日本支部合同大会（第481回講演会）, Sep. 2013, Japanese, 日本農芸化学会, 広島市, Domestic conference

  Oral presentation


• Characterization of cellulose nanofiber sheets from various grass plants

  延田 紘治, 寺村 浩, OGINO CHIAKI, KONDO AKIHIKO, NISHINO TAKASHI, 平野 恒, 佐塚 隆志, 北野 英己

  平成２５年度繊維学会秋季研究発表会, Sep. 2013, Japanese, 名古屋市, Domestic conference

  Poster presentation


• 稲わら希硫酸処理液のナノフィルトレーションによる糖濃縮

  SASAKI KENGO, HASUNUMA TOMOHISA, OGINO CHIAKI, KONDO AKIHIKO

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• 易糖化性を示すイネの探索

  寺村 浩, 平野 恒, 松田 史生, OGINO CHIAKI, 佐塚 隆志, 北野 英己, KONDO AKIHIKO

  第31回日本植物細胞分子生物学会大会;シンポジウム, Sep. 2013, Japanese, 日本植物細胞分子生物学会, 札幌市, Domestic conference

  Oral presentation


• バイオリファイナリーを目指したイオン液体の活用

  石田 亘広, 齋藤 聡志, 神谷 典穂, OGINO CHIAKI

  化学工学会第45回秋季大会, Sep. 2013, Japanese, 化学工学会, 岡山市, Domestic conference

  Poster presentation


• バイオマス分解性向上を目的とする酸導入イオン液体を用いた新規前処理法開発

  小倉 一真, OGINO CHIAKI, KONDO AKIHIKO

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• ナノ粒子とX線照射を併用した深部ガンの非侵襲的治療法の開発

  田野 亜都里, 森田 健太, OGINO CHIAKI, 佐藤 和好, 沼子 千弥, 中山 雅央, 佐々木 良平, KONDO AKIHIKO

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• コリネ型細菌を用いたプロトカテク酸の生産

  OKAI NAOKO, 竹嶋 康誠, OGINO CHIAKI, KONDO AKIHIKO

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• Characterization of cellulose nanofiber sheets from various grass plants

  延田 紘治, 寺村 浩, OGINO CHIAKI, KONDO AKIHIKO, NISHINO TAKASHI, 平野 恒, 佐塚 隆志, 北野 英己

  セルロース学会第20回年次大会, Jul. 2013, Japanese, 京都市, Domestic conference

  Poster presentation


• 植物源を異にするセルロースナノファイバーシートの構造と物性

  延田 紘治, 平野 恒, 佐塚 隆志, 北野 英己, 寺村 浩, OGINO CHIAKI, KONDO AKIHIKO, NISHINO TAKASHI

  第2回JACI/GSC 講演会, Jun. 2013, Japanese, 新化学技術推進協会, 大阪市, Domestic conference

  Poster presentation


• イオン液体を用いたバイオマスの前処理と同時糖化発酵

  OGINO CHIAKI, KONDO AKIHIKO

  第2回JACI/GSC 講演会, Jun. 2013, Japanese, 新化学技術推進協会, 大阪市, Domestic conference

  Poster presentation


• 重合性界面活性剤による難溶性材料可溶化技術の開発

  本庄 崇文, 宝得 一貴, MINAMI HIDETO, 竹中 武蔵, OGINO CHIAKI, Maruyama Tatsuo

  化学工学会 第78年会, Mar. 2013, Japanese, 化学工学会, 大阪大学, Domestic conference

  Oral presentation


• 原子間力顕微鏡を用いた細胞表層解析法および細胞応答解析法の開発

  竹中 武蔵, OGINO CHIAKI

  日本化学会第93春季年会, Mar. 2013, Japanese, 日本化学会, 草津市, Domestic conference

  Oral presentation


• Ethanol production from ionic liquid pretreated biomass by recombinant yeast

  OGINO Chiaki, KONDO Akihiko

  化学工学会第78年会, Mar. 2013, Japanese, 化学工学会, 豊中市, Domestic conference

  Oral presentation


• コリン系イオン液体と超音波を組み合わせた木質系バイオマス前処理

  仁宮一章, OGINO CHIAKI

  第３回イオン液体討論会, Dec. 2012, Japanese, イオン液体研究会, 那覇市, Domestic conference

  Oral presentation


• イオン液体耐性セルラーゼの単離と酵素特性解析

  窪田 有華, 仲島 菜々実, KUDOU MOTONORI, 中島 一紀, 岡﨑 文美, OGINO CHIAKI

  第３回イオン液体討論会, Dec. 2012, Japanese, イオン液体研究会, 那覇市, Domestic conference

  Oral presentation


• Interaction survey between GPCR and ligand on the surface of yeast by AFM

  Musashi Takenaka, Tomokazu Amino, Yusuke Miyachi, Jun Ishii, Chiaki Ogino, Akihiko Kondo

  International Joint Symposium on Single-Cell Analysis (The 6th International Workshop on Approaches to Single-Cell Analysis & The 8th International Forum on Post-Genome Technologies), Nov. 2012, English, The Society for Single-Cell Surveyor, 京都市, International conference

  Poster presentation


• 放線菌バイオマス分解酵素ライブラリの構築及び新規高分泌シグナルの探索

  宮崎 貴也, 野田 修平, 田中 勉, OGINO CHIAKI

  第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference

  Oral presentation


• ピリジニウム系イオン液体による結晶性セルロースのin situ 酵素糖化

  中元 亜耶, 石田 亘広, OGINO CHIAKI

  酵素工学研究会第６８回講演会, Oct. 2012, Japanese, 酵素工学研究会, 文京区, Domestic conference

  Poster presentation


• エンドグルカナーゼ分泌生産型放線菌によるセルロースからの安息香酸生産

  野田 修平, 田中 勉, OGINO CHIAKI

  第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference

  Oral presentation


• エンドグルカナーゼ分泌生産型放線菌によるセルロースからの安息香酸生産

  野田 修平, 田中 勉, OGINO CHIAKI

  酵素工学研究会第６８回講演会, Oct. 2012, Japanese, 酵素工学研究会, 文京区, Domestic conference

  Poster presentation


• イオン液体耐性セルラーゼの探索と機能解析

  窪田 有華, 山口 剛示, 仲島 菜々実, KUDOU MOTONORI, 中島 一紀, 岡﨑 文美, OGINO CHIAKI

  第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference

  Oral presentation


• イオン液体耐性セルラーゼの単離と酵素特性解析

  窪田 有華, KUDOU MOTONORI, 仲島 菜々実, 中島 一紀, 岡﨑 文美, OGINO CHIAKI

  酵素工学研究会第６８回講演会, Oct. 2012, Japanese, 酵素工学研究会, 文京区, Domestic conference

  Poster presentation


• イオン液体前処理による木質系バイオマスからのエタノール生産

  石田 亘広, 片平 悟史, 齋藤 聡志, 神谷 典穂, OGINO CHIAKI

  第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference

  Oral presentation


• イオン液体前処理とキシロース資化性酵母株を用いたバイオマス糖化発酵プロセスの効率化

  小倉 一真, 仲島 菜々実, YAMADA RYOSUKE, HASUNUMA TOMOHISA, 神谷 典穂, 石田 亘広, 齋藤 聡志, OGINO CHIAKI

  第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference

  Oral presentation


• Genetic engineering of valine biosynthesis for isobutanol production in Saccharomyces cerevisiae

  Jun Ishii, Fumio Matsuda, Kengo Ida, Hironori Tezuka, Takashi Kondo, Chiaki Ogino, Akihiko Kondo

  The 18th Symposium of Young Asian Biochemical Engineers' Community (YABEC2012), Oct. 2012, English, Society for Biological Engineering, Tokushima, Japan, International conference

  Oral presentation


• Direct ethanol production from lignocellulosic biomass pretreated with ionic liquid from cellulase-displaying yeast

  OGINO Chiaki

  The 18th Symposium of Young Asian Biochemical Engineers' Communyty, Oct. 2012, English, ㈳日本生物工学会, 徳島市, International conference

  Poster presentation


• Cell SELEX 法により選抜したヒト肝臓由来がん細胞に対するDNA アプタマーの評価

  川嶋 聡, 仁宮 一章, OGINO CHIAKI

  第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference

  Oral presentation


• Complex carriers of affibody-displaying bio-nanocapsule and composition-varied liposomes for HER2-expressing breast cancer cell-specific protein delivery

  Yuya Nishimura, Jun Ishii, Fumiyoshi Okazaki, Chiaki Ogino, Akihiko Kondo

  The 15th International Biotechnology Symposium (IBS 2012), Sep. 2012, English, The Korean Society for Biotechnology and Bioengineering, Deague, Korea, International conference

  Oral presentation


• Genetic engineering to enhance the Ehrlich pathway and alter carbon flux for increased isobutanol production by Saccharomyces cerevisiae

  Jun Ishii, Takashi Kondo, Hiroyuki Tezuka, Fumio Matsuda, Chiaki Ogino, Akihiko Kondo

  Metabolic EngineeringⅨ, Jun. 2012, English, Engineering Conferences International, Biarritz, France, International conference

  Oral presentation


• AFMを用いた新規核酸分子の探索

  OGINO CHIAKI

  21世紀を拓くバイオテクノロジーシンポジウム, Jun. 2012, Japanese, YABEC2012実行委員会, 徳島市, Domestic conference

  Oral presentation


• ラジカルを発生する金属酸化物粒子に対するXAFSによる非破壊状態分析

  沼子 千弥, 佐藤 和好, 高見 誠一, 梅津 光央, 田中 勉, OGINO CHIAKI

  第72回分析化学討論会, May 2012, Japanese, 日本分生化学会, 鹿児島市, Domestic conference

  Oral presentation


• DNA aptamer screening and evaluation by AFM

  OGINO Chiaki, AMINO Tomokazu, KONDO Akihiko

  The 12th Japan-China-Korea Joint Symposium on Enzyme Engineering, May 2012, English, 酵素工学研究会, 金沢市, International conference

  Oral presentation


• 動的周期操作を用いたエタノール連続発酵プロセスにおける分岐現象

  HAMASHIMA Naoko, HORIE Takafumi, OGINO Chiaki, OHMURA Naoto

  化学工学会第77年会, Mar. 2012, Japanese, 化学工学会, 東京, Domestic conference

  Oral presentation


• 麹菌Aspergillus oryzaeを用いたラクダ科動物由来一本鎖抗体可変部位VHH分泌生産系の構築

  吉栄 俊秀, 青木 淳一, 田淵 聡一郎, 岡崎 文美, 荻野 千秋, 田中 勉, 久田 博元, 秦 洋二, 近藤 昭彦

  日本農芸化学会2012年度大会, Mar. 2012, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• 原子間力顕微鏡を用いた細胞表層におけるレセプター・リガンド間相互作用測定系の構築

  竹中 武藏, 荻野 千秋, 宮地 佑典, 近藤 昭彦

  日本化学会第92春季年会, Mar. 2012, Japanese, 日本化学会, 横浜市, Domestic conference

  Oral presentation


• 海洋性超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの構造安定性

  岡崎 文美, 仲島 菜々実, 荻野 千秋, 近藤 昭彦

  日本農芸化学会2012年度大会, Mar. 2012, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• 海洋性超好熱菌Thermotoga neapolitana由来β-1,3-キシラナーゼの耐熱性を導く要因の解析

  山崎 清志, 岡崎 文美, 荻野 千秋, 近藤 昭彦, 三上 文三, 榑林 陽一, 鶴田 宏樹

  日本農芸化学会2012年度大会, Mar. 2012, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• ピリジニウム系イオン液体による結晶性セルロースの前処理・酵 素糖化・高速発酵(その2)

  荻野 千秋, 山口 剛示, 仲島 菜々美, 片平 悟史, 石田 亘広, 徳原 渡, 神谷 典穂, 近藤 昭彦

  化学工学会第77年会, Mar. 2012, Japanese, 化学工学会, 新宿区, Domestic conference

  Oral presentation


• ピリジニウム系イオン液体による結晶性セルロースの前処理・酵素糖化・高速発酵(その1)

  中元 亜耶, 庄田 靖宏, 引野 幸枝, 片平 悟史, 石田 亘広, 徳原 渡, 荻野 千秋, 神谷 典穂

  化学工学会第77年会, Mar. 2012, Japanese, 化学工学会, 新宿区, Domestic conference

  Oral presentation


• ソマトスタチンに対する新規DNA アプタマーの選抜およびセンシングへの応用

  荻野 千秋, 網野 智一, 宮地 佑典, 近藤 昭彦

  日本化学会第92春季年会, Mar. 2012, Japanese, 日本化学会, 横浜市, Domestic conference

  Oral presentation


• セルロースからの高効率一段階エタノール発酵を可能にする高機能酵母の創製

  中谷 侑紀, 山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

  化学工学会第77年会, Mar. 2012, Japanese, 化学工学会, 新宿区, Domestic conference

  Oral presentation


• コリネ型細菌を用いたプトレシンの生産

  岡井 直子, 畑田 一樹, 高橋 千尋, 荻野 千秋, 近藤 昭彦

  日本農芸化学会2012年度大会, Mar. 2012, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• エンドグルカナーゼ分泌生産型放線菌を用いたセルロースからの有用タンパク質生産

  河井 禎文, 野田 修平, 田中 勉, 荻野 千秋, 近藤 昭彦

  日本農芸化学会2012年度大会, Mar. 2012, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• エンドグルカナーゼ分泌生産型放線菌によるセルロースからの安息香酸生産

  野田 修平, 田中 勉, 荻野 千秋, 近藤 昭彦

  化学工学会第77年会, Mar. 2012, Japanese, 化学工学会, 新宿区, Domestic conference

  Oral presentation


• リグノセルロース・リファイナリーにおけるイオン液体の役割

  仁宮 一章, 荻野 千秋, 高橋 憲司

  第2回イオン液体討論会, Dec. 2011, Japanese, イオン液体研究会, 京都市, Domestic conference

  Oral presentation


• イオン液体耐性セルラーゼの探索と機能評価

  窪田 有華, 山口 剛示, 仲島 菜々実, 中島 一紀, 岡﨑 文美, 荻野 千秋, 近藤 昭彦

  第2回イオン液体討論会, Dec. 2011, Japanese, イオン液体研究会, 京都市, Domestic conference

  Oral presentation


• イオン液体前処理バイオマスを用いたエタノール同時糖化発酵

  荻野 千秋, 中島 一紀, 仲島 菜々美, 神谷 典穂, 徳原 渡, 石田 亘広, 片平 悟史, 近藤 昭彦

  第2回イオン液体討論会, Dec. 2011, Japanese, イオン液体研究会, 京都市, Domestic conference

  Oral presentation


• イオン液体前処理バイオマスの構造解析およびエタノール発酵

  山口 剛示, 中島 一紀, 荻野 千秋, 山田 亮祐, 堀江 孝史, 桑本 滋生, 横山 和司, 松井 純爾

  第2回イオン液体討論会, Dec. 2011, Japanese, イオン液体研究会, 京都市, Domestic conference

  Oral presentation


• イオン液体共存下における酵母のキシロース発酵能評価

  小倉 一真, 仲島 菜々実, 山田 亮祐, 蓮沼 誠久, 荻野 千秋, 近藤 昭彦

  第2回イオン液体討論会, Dec. 2011, Japanese, イオン液体研究会, 京都市, Domestic conference

  Oral presentation


• Amplification and smoothing of signal activation from human G-protein-coupled receptor for enriching agonist stimulation in yeast

  小田 麻美, 石井 純, 福田 展雄, 藤原 俊伸, 荻野 千秋, 近藤 昭彦

  第34回日本分子生物学会年会, Dec. 2011, English, 日本分子生物学会, 横浜市, Domestic conference

  Poster presentation


• 超好熱性細菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの結晶構造解析

  岡崎 文美, 山崎 清志, 荻野 千秋, 近藤 昭彦, 三上文三, 榑林陽一, 鶴田宏樹

  極限環境生物学会2011年度(第12回)年会, Nov. 2011, Japanese, 極限環境生物学会, 長崎市, Domestic conference

  Poster presentation


• 超好熱性細菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの安定性解析

  岡崎 文美, 仲島 菜々実, 荻野 千秋, 近藤 昭彦

  極限環境生物学会2011年度(第12回)年会, Nov. 2011, Japanese, 極限環境生物学会, 長崎市, Domestic conference

  Poster presentation


• 組換え麹菌生産セルラーゼを用いた広葉樹クラフトパルプの酵素糖化

  吉栄 俊秀, 酒井 翔司, 岡﨑 文美, 荻野 千秋, 田中 勉, 久田 博元, 萩原 伸哉, 秦 洋二, 近藤 昭彦

  第11回糸状菌分子生物学コンファレンス(2011), Nov. 2011, Japanese, 糸状菌分子生物学研究会, 文京区, Domestic conference

  Poster presentation


• ラクダ由来一本鎖抗体可変部位 VHH の麹菌 Aspergillus oryzae による分泌生産

  青木 淳一, 田淵 聡一郎, 岡﨑 文美, 荻野 千秋, 田中 勉, 久田 博元, 秦 洋二, 近藤 昭彦

  第11回糸状菌分子生物学コンファレンス(2011), Nov. 2011, Japanese, 糸状菌分子生物学研究会, 文京区, Domestic conference

  Poster presentation


• 放線菌が有する炭素源資化能力を生かしたタンパク質の大量分泌生産

  宮崎 貴也, 野田 修平, 田中 勉, 荻野 千秋, 近藤 昭彦

  化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋市, Domestic conference

  Poster presentation


• 組換え酵母による高効率グルタチオン生産

  桐山 健太郎, 原 清敬, 仲山 英樹, 荻野 千秋, 近藤 昭彦

  第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference

  Oral presentation


• 生体分子融合ナノ粒子の生物化学工学領域への高度応用に関する研究

  荻野 千秋

  第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference

  [Invited]

  Invited oral presentation


• 麹菌Aspergillus oryzae による超好熱菌Thermotoga neapolitana 由来耐熱性β-1,4- マンナナーゼの生産

  岡﨑 文美, 仲島 菜々実, 久田 博元, 荻野 千秋, 石田 博樹, Yopi, 秦 洋二, 近藤 昭彦

  第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference

  Oral presentation


• 酵母によるバイオマスからのグルタチオンの直接発酵

  原 清敬, 吉田 秀世, 桐山 健太郎, 金 松希, 荻野 千秋, 近藤 昭彦

  第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference

  Oral presentation


• 稲わらバイオマス形質の系統間比較

  松田 史生, 山崎 将紀, 荻野 千秋, 近藤 昭彦

  第29回日本植物細胞分子生物学会大会, Sep. 2011, Japanese, 日本植物細胞分子生物学会, 福岡市, Domestic conference

  Oral presentation


• βグルコシダーゼを表層提示したセロオリゴ糖資化性大腸菌の創製

  田中 勉, 川畑 瞳, 平田 有希, 荻野 千秋, 近藤 昭彦

  化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋市, Domestic conference

  Poster presentation


• セルラーゼ分泌酵母によるイオン液体処理バイオマスからのエタノール生産

  片平 悟史, 中村 里沙, 徳原 渡, 則武 義幸, 中島 一紀, 神谷 典穂, 荻野 千秋, 石田 亘広

  第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference

  Oral presentation


• セルラーゼ発現最適化二倍体酵母による木質系バイオマスからのエタノール発酵

  山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

  化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋市, Domestic conference

  Poster presentation


• イオン液体処理が結晶性セルロース酵素糖化に与える影響

  中元 亜耶, UJU, 庄田 靖宏, 後藤 雅宏, 石田 亘広, 徳原 渡, 則武 義幸, 荻野 千秋, 神谷 典穂

  第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference

  Oral presentation


• イオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発

  荻野 千秋, 中島 一紀, 山口 剛示, 窪田 有華, 片平 悟史, 石田 亘広, 徳原 渡, 則武 義幸, 近藤 昭彦

  第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference

  Oral presentation


• Corynebacterium glutamicumを用いた高効率なGABA生産技術の開発

  高橋 千尋, 白川 順規, 畑田 一樹, 岡井 直子, 田中 勉, 荻野 千秋, 近藤 昭彦

  化学工学会第43回秋季大会, Sep. 2011, Japanese, 化学工学会, 名古屋市, Domestic conference

  Poster presentation


• BGL 表層提示大腸菌によるセロビオースを基質としたIPA 生産

  相馬 悠希, 猪熊 健太郎, 田中 勉, 荻野 千秋, 近藤 昭彦, 花井 泰三

  第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference

  Oral presentation


• Affibody 提示Bio-nanocapsule を用いたER2 発現癌細胞へのタンパク質送達システム

  西村 勇哉, 三村 和起子, 竹田 光一, 石井 純, 岡崎 文美, 荻野 千秋, 近藤 昭彦

  第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference

  Oral presentation


• Aromatic chemicals production using phenylalnine ammonia lyase expressing Streptomyces lividans

  OGINO Chiaki

  Defence, Science & Research (DSR 2011) Conference, Aug. 2011, English, Committee of DSR2011, Singapore, International conference

  Oral presentation


• バイオナノカプセルを用いた細胞特異的ドラッグデリバリーシステム

  近藤 昭彦, 田中 勉, 荻野 千秋

  第27回日本DDS学会学術集会, Jun. 2011, Japanese, 日本DDS学会, 文京区, Domestic conference

  Oral presentation


• Key Monomers Production from Biomass Toward Bio-based Polymers Using

  OGINO Chiaki

  International Conference on Materials for Asdvanced Technologies 2011, Jun. 2011, English, Materials Research Society Singapore, Singapore, International conference

  Oral presentation


• イオン液佒へのセルロースの溶解とバイオエタノール生産

  中島 一紀, 山口 剛示, 片平 悟史, 石田 亖広, 荻野 千秋, 近藤 昭彦

  第60回高分子学会年次大会, May 2011, Japanese, 高分子学会, 大阪市, Domestic conference

  Oral presentation


• Homo D-lactic acid fermentation from pentose and xylan by metabolically engineered Lactobacillus plantarum

  TANAKA Tsutomu, OKANO Kenji, OGINO Chiaki, KONDO Akihiko

  33rd Symposium on Biotechnology for Fuels and Chemicals, May 2011, English, Society for industrial Microbiology, Seattle, USA, International conference

  Poster presentation


• Effective Screening of Optimized Diploid Saccharomyces cerevisiae Strain for Direct Ethanol Production from Cellulosic Materials by Displaying Ratio Adjustment of Cellulolytic Enzymes

  OGINO Chiaki

  2011 International Meeting of the Microbiological Society of Korea, May 2011, English, The Microbiological Society of Korea, Gwangju, Korea, International conference

  Oral presentation


• Cinnamic acid production from various carbon sources using phenylalnine ammonia lyase expressing Streptomyces lividans

  NODA Shuhei, MIYOSHI, Takanori, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  33rd Symposium on Biotechnology for Fuels and Chemicals, May 2011, English, Society for industrial Microbiology, Seattle, USA, International conference

  Poster presentation


• Characterization of Titanium oxide nano-particle suspension by XAFS technique

  NUMAKO C, SATO K, OHARA S, UMETSU M, TAKAMI S, SASAKI R, OGINO Chiaki, KONDO Akihiko

  E-MRS ICAM IUMRS 2011 Spring Meeting, May 2011, English, European Materials Research Society, Nice, France, International conference

  Oral presentation


• 放線菌を用いたあらゆる炭素源からのケイ皮酸生産

  野田 修平, 三好 孝則, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference

  Oral presentation


• 放線菌によるトランスグルタミナーゼの大量分泌生産に向けた生産条件の検討

  宮崎 貴也, 野田 修平, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• 放線菌によるトランスグルタミナーゼの生産

  宮崎 貴也, 野田 修平, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference

  Oral presentation


• 放線菌における表層提示技術に関する研究

  中村 悠佑, 野田 修平, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference

  Oral presentation


• 長期エタノール発酵に向けたアミラーゼ表層提示発現酵母株の創製

  山川 瞬一, YAMADA Ryosuke, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference

  Oral presentation


• 代謝工学を活用したイソブタノール生産酵母の創製

  手塚 裕紀, KONDO Takashi, ISHII Jun, OGINO Chiaki, KONDO Akihiko

  化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference

  Oral presentation


• 組換え酵母による高効率グルタチオン生産

  桐山 健太郎, HARA Kiyotaka, NAKAYAMA Hideki, OGINO Chiaki, KONDO Akihiko

  日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• 酵母を用いたバイオマス資源からのグルタチオン生産技術の開発

  Kim Songhee, 吉田 秀世, HARA Kiyotaka, OGINO Chiaki, KONDO Akihiko

  化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference

  Oral presentation


• 酵母を用いたバイオマスからのグルタチオンの生産

  HARA Kiyotaka, 吉田 秀世, 桐山 健太郎, Kim Songhee, OGINO Chiaki, KONDO Akihiko

  化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference

  Oral presentation


• 酵素活性を制御するDNAアプタマーの探索

  宮地 佑典, OGINO Chiaki, KONDO Akihiko

  日本化学会第91春季年会, Mar. 2011, Japanese, 化学工学会, 横浜市, Domestic conference

  Oral presentation


• 原子間力顕微鏡を用いた機能性核酸分子の選抜

  奥村 雄三, OGINO Chiaki, 宮地 佑典, KONDO Akihiko

  日本化学会第91春季年会, Mar. 2011, Japanese, 化学工学会, 横浜市, Domestic conference

  Oral presentation


• 原子間力顕微鏡を用いた機能性核酸分子の選抜

  奥村 雄三, OGINO Chiaki, 宮地 佑典, KONDO Akihiko

  化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference

  Oral presentation


• 原子間力顕微鏡を用いたアミノ酸に対する機能性核酸分子・アプタマーの新規選抜法の開発

  OGINO Chiaki, 早瀬 太治, 宮地 佑典, KONDO Akihiko

  日本化学会第91春季年会, Mar. 2011, Japanese, 化学工学会, 横浜市, Domestic conference

  Oral presentation


• 過酸化チタンナノ粒子及び低線量X線照射を併用した深部ガンの非侵襲的治療法の開発

  森田 健太, 唐崎 美樹, 佐藤 和好, 大原 智, 中山 雅央, 佐々木 良平, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference

  Oral presentation


• 過酸化チタンナノ粒子及び低線量X線照射を併用した深部ガンの非侵襲的治療法の開発

  森田 健太, 唐崎 美樹, 佐藤 和好, 大原 智, 中山 雅央, 佐々木 良平, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference

  Oral presentation


• βグルコシダーゼ表層提示によるセロビオース資化性大腸菌の創製

  TANAKA Tsutomu, 川畑 瞳, OGINO Chiaki, KONDO Akihiko

  日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• βグルコシダーゼを表層提示したセロビオース資化性大腸菌の創製

  TANAKA Tsutomu, 川畑 瞳, OGINO Chiaki, KONDO Akihiko

  化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference

  Oral presentation


• ランダム変異導入による高活性エンドグルカナーゼの創製

  中谷 侑紀, YAMADA Ryosuke, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference

  Oral presentation


• トランスポゾンタギング法による好塩性細菌Halomonas elongata OUT30018株の浸透圧ストレス応答性遺伝子の探索

  谷村 幸亮, NAKAYAMA Hideki, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference

  Oral presentation


• ソマトスタチンに対する新規DNAアプタマーの選抜

  網野 智一, OGINO Chiaki, 宮地 佑典, KONDO Akihiko

  日本化学会第91春季年会, Mar. 2011, Japanese, 化学工学会, 横浜市, Domestic conference

  Oral presentation


• コリネ型細菌を用いたGABAの生産

  高橋 千尋, 白川 順規, 畑田 一樹, 土舘 健幸, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• イオン液体共存下でのセルロース系バイオマスからの直接エタノール発酵

  NAKASHIMA Kazunori, 谷口 菜穂, 山口 剛示, YAMADA Ryosuke, 片平 悟史, 石田 亘広, 徳原 渡, 則武 義幸, OGINO Chiaki, KONDO Akihiko

  日本化学会第91春季年会, Mar. 2011, Japanese, 化学工学会, 横浜市, Domestic conference

  Oral presentation


• イオン液体を利用したバイオマスの前処理・糖化・発酵技術

  石田 亘広, 片平 悟史, 中村 里沙, 徳原 渡, 則武 義幸, 神谷 典穂, NAKASHIMA Kazunori, OGINO Chiaki, KONDO Akihiko, 高橋 治雄

  日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• イオン液体をベースとしたセルロース系バイオマスの前処理とエタノール発酵プロセス

  NAKASHIMA Kazunori, 谷口 菜穂, 山口 剛示, YAMADA Ryosuke, 片平 悟史, 石田 亘広, 徳原 渡, 則武 義幸, OGINO Chiaki, KONDO Akihiko

  化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference

  Oral presentation


• イオン液体によるバイオマスの前処理・糖化・発酵技術の開発

  石田 亘広, 片平 悟史, 中村 里沙, 徳原 渡, 則武 義幸, 神谷 典穂, NAKASHIMA Kazunori, OGINO Chiaki, KONDO Akihiko, 高橋 治雄

  化学工学会第76年会, Mar. 2011, Japanese, 日本化学会, 東京都 小金井市, Domestic conference

  Oral presentation


• Streptomyces griseocarneusスフィンゴミエリナーゼCの速度論的解析とMg2+による活性増強メカニズムの解明

  冨田 優, 松本 優作, 杉森 大助, 村山 和隆, OGINO Chiaki

  日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• Single-cell analysis revealed correlation between yeast GPCR signaling and plasmid retention

  Jun Ishii, Chiaki Ogino, Akihiko Kondo

  The 5th International Workshop on Approaches to Single-Cell Analysis, Mar. 2011, English, Tokyo, Japan, International conference

  Poster presentation


• Corynebacterium glutamicumを用いたGABAの高効率生産技術の開発

  高橋 千尋, 白川 順規, 畑田 一樹, OKAI Naoko, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference

  Oral presentation


• AFMを用いた生細胞表層のレセプター・リガンド間相互作用解析と細胞応答の観察

  OGINO Chiaki, 野坂 和輝, ISHII Jun, 宮地 佑典, KONDO Akihiko

  日本化学会第91春季年会, Mar. 2011, Japanese, 化学工学会, 横浜市, Domestic conference

  Oral presentation


• 植物から燃料を作る

  OGINO Chiaki

  芦屋川カレッジ, Feb. 2011, Japanese, 芦屋市公民館, 芦屋市, Domestic conference

  Invited oral presentation


• 金属酸化ナノ粒子と物理的励起を併用したラジカル発生システムを用いた細胞損傷システムの構築

  OGINO Chiaki

  第23回CES21講演会, Feb. 2011, Japanese, 日本分子生物学会, 大阪市, Domestic conference

  Invited oral presentation


• High-resolution, quantitative signalling assay for G-protein-coupled receptors by flow cytometry

  Akihiko Kondo, Jun Ishii, Nobuo Fukuda, Chiaki Ogino

  The 2010 International Chemical Congress of Pacific Basin Societies (Pacifichem 2010), Dec. 2010, English, Honolulu, Hawaii, USA, International conference

  Oral presentation


• 麹菌A. oryzaeによる海洋細菌由来β-1,3-キシラナーゼの生産

  久田 博元, OKAZAKI Fumiyoshi, 石田 博樹, OGINO Chiaki, 秦 洋二, KONDO Akihiko

  第10回 糸状菌分子生物学コンファレンス, Nov. 2010, Japanese, 糸状菌分子生物学研究会, 東広島市, Domestic conference

  Poster presentation


• 麹菌A. oryzaeにより生産した海洋細菌由来β-1,3-キシラナーゼの酵素学的諸性質

  OKAZAKI Fumiyoshi, 仲島 菜々実, 久田 博元, OGINO Chiaki, 石田 博樹, 秦 洋二, KONDO Akihiko

  第10回 糸状菌分子生物学コンファレンス, Nov. 2010, Japanese, 糸状菌分子生物学研究会, 東広島市, Domestic conference

  Poster presentation


• Selection of DNA aptamers using Atomic Force Microscopy

  OGINO Chiaki

  The 16th Symposium of Young Asian Biochemical Engineers' Communyty, Nov. 2010, English, (社)化学工学会, 台湾桃園県, International conference

  Oral presentation


• Ionic liquid Pretreatment for Direct Ethanol Production from Cellulosic Material by Cellulase-displaying Arming Yeast

  OGINO Chiaki, YAMAGUCHI, Koji, NAKASHIMA Kazunori, KONDO Akihiko

  International Conference on Sustainable Biomass (ICSB) 2010, Nov. 2010, English, 不明, Tamsui, Taiwan, International conference

  Oral presentation


• 放線菌を用いた様々なタンパクの大量分泌系の構築

  野田 修平, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• 超好熱菌Thermotoga neapolitana 由来耐熱性β -1,3- キシラナーゼの機能解析

  OKAZAKI Fumiyoshi, 仲島 菜々実, 久田 博元, OGINO Chiaki, 石田 博樹, 秦 洋二, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• 麹菌による超好熱菌Thermotoga neapolitana 由来耐熱性β -1,3- キシラナーゼの高分泌生産

  久田 博元, OKAZAKI Fumiyoshi, 石田 博樹, OGINO Chiaki, 秦 洋二, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• 過酸化チタンナノ粒子及び低線量X 線照射を併用した深部ガンの非侵襲的治療法の開発

  唐崎 美樹, 松井 かずさ, 佐藤 和好, 大原 智, 佐々木 良平, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• セルラーゼ発現バランス最適化酵母を用いたセルロースからのエタノール発酵

  YAMADA Ryosuke, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• イオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発(その4)

  NAKASHIMA Kazunori, 谷口 菜穂, 山口 剛示, 片平 悟史, 石田 亘広, 高橋 治雄, 田端 一英, 則武 義幸, OGINO Chiaki, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• イオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発(その3)

  山口 剛示, NAKASHIMA Kazunori, YAMADA Ryosuke, OGINO Chiaki, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• イオン液体を利用したバイオマスの前処理・糖化・発酵技術の開発(その2)

  石田 亘広, 片平 悟史, 中村 里沙, 田端 一英, 則武 義幸, 神谷 典穂, NAKASHIMA Kazunori, OGINO Chiaki, KONDO Akihiko, 高橋 治雄

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• Cell SELEX 法によるヒト肝臓由来がん細胞に選択的に結合するDNA アプタマーの選抜

  仁宮 一章, 金田 壱彦, OGINO Chiaki, 清水 宣明

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• Affibody 提示ナノカプセルを用いたHER2 発現癌細胞特異的な薬物送達

  三枝 宏彰, 宍戸 卓矢, 西村 勇哉, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• 1細胞アッセイ技術に基づくGPCR 解析、アゴニスト探索法の開発

  KONDO Akihiko, ISHII Jun, OGINO Chiaki

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Oral presentation


• 力学的指標による生細胞表面におけるリガンド‐受容体の相互作用解析

  野坂 和輝, 宮地 佑典, ISHII Jun, OGINO Chiaki, KONDO Akihiko

  化学工学会第42回秋季大会, Sep. 2010, Japanese, (社)化学工学会, 京都市, Domestic conference

  Poster presentation


• 放線菌を用いた様々なタンパクの大量分泌系の構築

  野田 修平, TANAKA Tsutomu, KONDO Akihiko, OGINO Chiaki

  2010年度日本放線菌学会大会, Sep. 2010, Japanese, 日本放線菌学会, 東京都 江戸川区, Domestic conference

  Oral presentation


• 超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの発現および諸性質

  OKAZAKI Fumiyoshi, 仲島 菜々実, 久田 博元, OGINO Chiaki, 石田 博樹, 秦 洋二, KONDO Akihiko

  日本応用糖質科学会 平成22年度大会(第59回), Sep. 2010, Japanese, 日本応用糖質科学会, 静岡市, Domestic conference

  Oral presentation


• 麹菌(Aspergillus oryzae)を宿主とした超好熱菌Thermotoga neapolitana由来耐熱性β-1,3-キシラナーゼの分泌高生産

  久田 博元, OKAZAKI Fumiyoshi, 石田 博樹, OGINO Chiaki, 秦 洋二, KONDO Akihiko

  日本応用糖質科学会 平成22年度大会(第59回), Sep. 2010, Japanese, 日本応用糖質科学会, 静岡市, Domestic conference

  Oral presentation


• 高効率バイオディーゼル生産を目的としたWhole-cell biocatalystによる新規リパーゼ複合系の開発

  足立 大輔, NAKASHIMA Kazunori, OGINO Chiaki, KONDO Akihiko

  化学工学会第42回秋季大会, Sep. 2010, Japanese, (社)化学工学会, 京都市, Domestic conference

  Poster presentation


• 酵母によるエタノール発酵に及ぼす撹拌剪断効果

  内田 敦子, 伊藤 友教, 堀田 淳史, Horie, Takafumi, OGINO Chiaki, OHMURA Naoto

  化学工学会第42回秋季大会, Sep. 2010, Japanese, (社)化学工学会, 京都市, Domestic conference

  Poster presentation


• 機能性酵母によるバイオエタノール生産プロセスの強化

  伊藤 友教, 内田 敦子, 堀田 淳史, Horie, Takafumi, OGINO Chiaki, OHMURA Naoto

  化学工学会第42回秋季大会, Sep. 2010, Japanese, (社)化学工学会, 京都市, Domestic conference

  Poster presentation


• affibody提示バイオナノカプセルを用いたタンパク質デリバリーシステム

  西村 勇哉, 三枝 宏彰, TANAKA Tsutomu, OGINO Chiaki, KONDO Akihiko

  化学工学会第42回秋季大会, Sep. 2010, Japanese, (社)化学工学会, 京都市, Domestic conference

  Poster presentation


• Construction of consolidated bioprocesses for production of bio-fuels and chemicals by using cell surface engineered microbial cells

  KONDO Akihiko, HASUNUMA Tomohisa, OGINO Chiaki, TANAKA Tsutomu

  RENEWABLE ENERGY 2010, Jun. 2010, English, International Solar Energy Society, 横浜市, International conference

  Oral presentation


• Intensification of Bioethanol Fermentation by Recombinant Yeast with Xylose Isomerase Pathway

  ITO, T, HOTTA, A, UCHIDA, A, TANINO, T, OGINO Chiaki, KONDO Akihiko, OHMURA Naoto

  Industrial Biotechnology International Conference 2010, Apr. 2010, English, The Italian Association of Chemical Engineering BIOTECH WORKING GROUP, Milano, Italy, International conference

  Oral presentation


• 放線菌を用いた様々なタンパクの大量分泌系の構築

  野田 修平, 岡井 直子, 田中 勉, 近藤 昭彦, 荻野 千秋

  化学工学会第75年会, Mar. 2010, Japanese, (社)化学工学会, 鹿児島市, Domestic conference

  Oral presentation


• 非侵襲性がん治療に向けた高機能性二酸化チタンナノ粒子の開発

  唐崎 美樹, 松井 かずさ, 瀬川 麻衣子, 田中 勉, 近藤 昭彦, 荻野 千秋

  化学工学会第75年会, Mar. 2010, Japanese, (社)化学工学会, 鹿児島市, Domestic conference

  Oral presentation


• 生デンプンからの効率的な繰返し発酵を達成するアミラーゼ発現新規酵母株の創製

  山川 瞬一, 山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

  化学工学会第75年会, Mar. 2010, Japanese, (社)化学工学会, 鹿児島市, Domestic conference

  Oral presentation


• 細胞表層工学技術を用いたヘミセルロース系バイオマスからのエタノール生産

  阪本 貴俊, 蓮沼 誠久, 堀 良美, 山田 亮祐, 荻野 千秋, 近藤 昭彦

  日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference

  Oral presentation


• 酵母を用いた生デンプンからの高効率な機能性ペプチド生産

  吉田 秀世, 桐山 健太郎, 荒井 章吾, 原 清敬, 荻野 千秋, 近藤 昭彦

  日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference

  Oral presentation


• 原子間力顕微鏡を用いた細胞表層におけるリガンド・レセプター間相互作用測定系の構築

  野坂 和輝, 荻野 千秋, 石井 純, 宮地 佑典, 近藤 昭彦

  日本化学会第90春季年会, Mar. 2010, Japanese, 日本化学会, 東大阪市, Domestic conference

  Oral presentation


• 原子間力顕微鏡を用いた機能性核酸分子・アプタマーの新規選抜法の開発

  早瀬 太治, 荻野 千秋, 宮地 佑典, 近藤 昭彦

  日本化学会第90春季年会, Mar. 2010, Japanese, 日本化学会, 東大阪市, Domestic conference

  Oral presentation


• 機能性酵母によるイオン液体前処理セルロースからのバイオエタノール生産

  中島 一紀, 荒井 章吾, 山口 剛示, 山田 亮祐, 片平 悟史, 石田 亘広, 高橋 治雄, 荻野 千秋, 近藤 昭彦

  日本化学会第90春季年会, Mar. 2010, Japanese, 日本化学会, 東大阪市, Domestic conference

  Oral presentation


• バイオマスからの燃料・化学品生産を目指した細胞工場の創製

  近藤 昭彦, 荻野 千秋, 蓮沼 誠久, 田中 勉

  日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference

  Oral presentation


• セルラーゼ表層提示酵母を用いたイオン液体前処理セルロースからの直接エタノール発酵

  中島 一紀, 荒井 章吾, 山口 剛示, 山田 亮祐, 片平 悟史, 石田 亘広, 高橋 治雄, 荻野 千秋, 近藤 昭彦

  化学工学会第75年会, Mar. 2010, Japanese, (社)化学工学会, 鹿児島市, Domestic conference

  Oral presentation


• カクテルδインテグレーション法によるセルラーゼ発現バランス最適化酵母の創製

  山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

  日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference

  Oral presentation


• DNAアプタマーを用いた酵素活性制御系の構築

  宮地 佑典, 清水 宣明, 荻野 千秋, 近藤 昭彦

  日本化学会第90春季年会, Mar. 2010, Japanese, 日本化学会, 東大阪市, Domestic conference

  Oral presentation


• AFMを用いたアプタマーによるタンパク質の検出・定量方法

  網野 智一, 荻野 千秋, 宮地 佑典, 近藤 昭彦

  日本化学会第90春季年会, Mar. 2010, Japanese, 日本化学会, 東大阪市, Domestic conference

  Oral presentation


• セルラーゼ提示超分子複合体提示酵母によるバイオマスの効率的分解

  荻野 千秋

  第32回日本分子生物学会年会, Dec. 2009, Japanese, 日本分子生物学会, 横浜市, Domestic conference

  Invited oral presentation


• Screening of DNA aptamers that recognize amino acid by AFM-SELEX strategy

  早瀬 太治, 宮地 佑典, 荻野 千秋, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Protein production for Bio-refinery strategy

  荻野 千秋, 清水 宣明, 福田 秀樹, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Production of biodiesel fuel in ionic liquids catalyzed by whole-cell biocatalysts

  中島 一紀, 荒井 章吾, 谷野 孝徳, 荻野 千秋, 近藤 昭彦, 福田 秀樹

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Oral presentation


• New SELEX strategy for screening of DNA aptamer by AFM

  宮地 佑典, 清水 宣明, 荻野 千秋, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Oral presentation


• Investigation of the interaction between GPCR and ligand by AFM equipped with bio-molecule modified cantilever

  NOSAKA K, MIYACHI Y, ISHII Jun, OGINO Chiaki, KONDO Akihiko

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Engineering of endoglucanase expression system for Corynebacterium glutamicum

  土舘 健幸, 舘野 俊博, 田中 勉, 荻野 千秋, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Efficient repeated batch fermentation from raw starch by novel yeast expressing transporter gene and amylase genes

  山川 瞬一, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Efficient homo D-lactic acid production from xylose

  吉田 渉悟, 岡野 憲司, 田中 勉, 荻野 千秋, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Efficient ethanol production from xylose by mated diploid Saccharomyces cerevisiae

  角山 裕昭, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Efficient D-lactic acid production from raw starch

  新川 智, 岡野 憲司, 田中 勉, 荻野 千秋, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Efficient and practical ethanol production from high yield rice by amylase expressing Saccharomyces cerevisiae

  山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Direct fermentation of cellulosic materials to ethanol using yeast strains codisplaying three types of cellulolytic enzyme

  柳瀬 修平, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Development of novel cell-surface display system of Aspergillus oryzae using chitin binding domain

  田渕 聡一郎, 伊藤 純二, 足立 崇, 石田 博樹, 秦 洋二, 田中 勉, 荻野 千秋, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Bioethaol fermentation from mixed sugar by the recombinant yeast with xyloseisomerase pathway

  TANINO Takanori, HOTTA Atsushi, ITO Tomonori, ISHII Jun, YAMADA Ryousuke, HASUNUMA Tomohisa, OGINO Chiaki, OHMURA Naoto

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Bioethanol production from mixed sugars using sugar uptake ability enhanced yeast strain by overexpression of transporters

  堀田 淳史, 谷野 孝徳, 伊藤 友数, 蓮沼 誠久, 荻野 千秋, 近藤 昭彦, 大村 直人

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Biodiesel production using whole-cell biocatalyst of Aspergillus oryzae coexpression lipases

  足立 大輔, 中島 一紀, 田中 勉, 荻野 千秋, 近藤 昭彦, 福田 秀樹

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Antibody-immobilized TiO2 nanoparticles for cancer therapy

  松井 かずさ, 瀬川 麻衣子, 田中 勉, 近藤 昭彦, 荻野 千秋

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Affibody displaying bionanocapsules for HER2 specific drug delivery

  西村 勇哉, 宍戸 卓矢, 三枝 宏彰, 田中 勉, 荻野 千秋, 近藤 昭彦

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• セルロース資源からのバイオエタノール生産技術

  荻野 千秋

  バイテクノロジー講演会, Oct. 2009, Japanese, バイオテクノロジー研究会, 福井市, Domestic conference

  Invited oral presentation


• 糖代謝ネットワークを強化した酵母による混合糖からのバイオエタノール生産

  HOTTA Atsushi, ITO Tomonori, UCHIDA Atsuko, TANINO Takanori, OGINO Chiaki, OHMURA Naoto

  化学工学会第41回秋季大会, Sep. 2009, Japanese, 化学工学会, 広島大学, Domestic conference

  Oral presentation


• 生デンプンから高効率にエタノールを生産する新規酵母の創製

  山川 瞬一, 山田 亮祐, 田中 勉, 荻野 千秋, 近藤 昭彦

  第61回日本生物工学会大会, Sep. 2009, Japanese, (社)日本生物工学会, 名古屋市, Domestic conference

  Oral presentation


• 菌体触媒を用いたバイオディーゼル生産におけるイオン液体の利用

  中島 一紀, 荒井 章吾, 谷野 孝徳, 荻野 千秋, 近藤 昭彦, 福田 秀樹

  第41回 化学工学会秋季大会, Sep. 2009, Japanese, (社)化学工学会, 東広島市, Domestic conference

  Oral presentation


• 癌細胞標的化を目指したAffibody 提示ナノカプセルの開発

  三枝 宏彰, 宍戸 卓矢, 田中 勉, 荻野 千秋, 近藤 昭彦

  第61回日本生物工学会大会, Sep. 2009, Japanese, (社)日本生物工学会, 名古屋市, Domestic conference

  Oral presentation


• 遺伝子組換え麹菌を用いたタンパク質大量分泌生産技術の開発

  酒井 翔司, 松田 哲弥, 足立 崇, 久田 博元, 秦 洋二, 田中 勉, 荻野 千秋, 近藤 昭彦

  第61回日本生物工学会大会, Sep. 2009, Japanese, (社)日本生物工学会, 名古屋市, Domestic conference

  Oral presentation


• ヘマトコッカス藻の高密度培養に対する二酸化チタン-超音波励起法の適用

  IWASAKI Daiki, SENGA Itaru, YAMASHITA Takashi, TONAI Masahiro, OGINO Chiaki, SHIMIZU Nobuaki, KATSUDA Tomohisa, YAMAJI Hideki

  化学工学会第41回秋季大会, Sep. 2009, Japanese, 化学工学会, 東広島市, Domestic conference

  Poster presentation


• Selection of DNA aptamers using atomic force microscopy

  荻野 千秋, 宮地 佑典, 早瀬 太治, 野坂 和輝, 近藤 昭彦

  Progress on Post Genome Technologies, Sep. 2009, English, Tsinghua, Southeast, Nanjing, Northwest University, Beijing, China, International conference

  Oral presentation


• Porin を用いたコリネ菌新規細胞表層提示技術の開発

  畑田 一樹, 舘野 俊博, 岡井 直子, 田中 勉, 荻野 千秋, 近藤 昭彦

  第61回日本生物工学会大会, Sep. 2009, Japanese, (社)日本生物工学会, 名古屋市, Domestic conference

  Oral presentation


• Development of environmentally benign and consolidated process for efficient production of cellulosic ethanol

  荻野 千秋

  SIM2009 Annual Meeting and Exhibition, Jul. 2009, English, Society for Industrial Microbiology, Toronto, Canada, International conference

  [Invited]

  Invited oral presentation


• 大豆イソフラボンからのイソフラボンアグリコンの効率的生産に適した新規βグルコシダーゼの開発

  荻野 千秋

  (財)不二たん白質研究振興財団第12回研究報告会, Jun. 2009, Japanese, (財)不二たん白質研究振興財団, 豊中市, Domestic conference

  Oral presentation


• 放線菌遺伝子組み換え系のタンパク質分泌生産への適用

  荻野 千秋, 伊藤 裕子, 宮下 徹, 仁宮 一章, 清水 宣明

  化学工学会 第75年会, Mar. 2009, Japanese, 化学工学会, 神奈川県横浜市, Domestic conference

  Oral presentation


• 非侵襲性がん治療のためのタンパク質修飾ナノ粒子の開発

  松井 かずさ, 荻野 千秋, 田中 勉, 福田 秀樹, 近藤 昭彦

  化学工学会 第74年会, Mar. 2009, Japanese, 化学工学会, 神奈川県横浜市, Domestic conference

  Oral presentation


• バイオエタノール生産を目指したスーパー酵母の創製

  近藤 昭彦, 蓮沼 誠久, 田中 勉, 荻野 千秋

  農芸化学会2009年度大会, Mar. 2009, Japanese, 日本農芸化学会, 福岡県福岡市, Domestic conference

  Oral presentation


• ソフトセルロース資源からのエタノール発酵技術の開発

  荻野 千秋, 近藤 昭彦

  日本草地学会, Mar. 2009, Japanese, 日本草地学会, 神奈川県藤沢市, Domestic conference

  Invited oral presentation


• キシロース資化性遺伝子組換え酵母の代謝プロファイリング解析

  WALUYO Danang, 阿部 祐一郎, 馬場 健史, 荻野 千秋, 小林 昭雄, 近藤 昭彦, 福崎 英一郎

  農芸化学会2009年度大会, Mar. 2009, Japanese, 日本農芸化学会, 福岡県福岡市, Domestic conference

  Poster presentation


• キシロースからの高効率エタノール生産を目指した宿主酵母株の検討

  角山 裕昭, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  農芸化学会2009年度大会, Mar. 2009, Japanese, 日本農芸化学会, 福岡県福岡市, Domestic conference

  Poster presentation


• アミラーゼ発現酵母による生デンプンからの効率的な繰り返し発酵プロセスの開発

  山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  農芸化学会2009年度大会, Mar. 2009, Japanese, 日本農芸化学会, 福岡県福岡市, Domestic conference

  Poster presentation


• TiO&2&ナノ粒子と超音波を併用した新規がん治療法構築のための基礎的検討

  仁宮 一章, 園家 史朗, 大島 周平, 荻野 千秋, 黒田 俊一, 清水 宣明

  化学工学会 第74年会, Mar. 2009, Japanese, 化学工学会, 神奈川県横浜市, Domestic conference

  Oral presentation


• 未来バイオ燃料生産用スーパー(CBP)微生物の開発

  近藤 昭彦, 荻野 千秋, 田中 勉

  第31回日本分子生物学会年会;第81回日本生化学会大会 合同大会, Dec. 2008, Japanese, 日本分子生物学学会, 日本生化学会, 神戸市, Domestic conference

  Oral presentation


• 二酸化チタンナノ粒子による活性酸素非依存的細胞死誘導

  高木 圭子, 樋口 善博, 橋井 美奈子, 荻野 千秋, 清水 宣明

  第31回日本分子生物学会年会・第82回日本生化学会大会 合同大会, Dec. 2008, Japanese, 日本分子生物学学会, 日本生化学会, 神戸市, Domestic conference

  Oral presentation


• 原子間力顕微鏡を用いたアプタマーの選抜

  宮地 佑典, 清水 宣明, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  若手フロンティア研究会2008, Dec. 2008, Japanese, 神戸大学研究基盤センター, 神戸市, Domestic conference

  Poster presentation


• 温度応答性磁性ナノ粒子を用いた迅速かつ高効率なアフィニティー分子の選択法の確立

  福田 展雄, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  若手フロンティア研究会2008, Dec. 2008, Japanese, 神戸大学研究基盤センター, 神戸市, Domestic conference

  Poster presentation


• Production of bio-fuels and chemicals from biomass by cell surface engineered yeast strains

  荻野 千秋, 近藤 昭彦

  4th International Symposium on Biocatalysis and Biotechnology, Nov. 2008, English, International Society of Biocatalysis and Biotechnology, Taipei, Taiwan, International conference

  [Invited]

  Invited oral presentation


• Methylester Synthesis By Fungi Immobilized BSPs in Ionic Liquid

  荒井 章吾, 荻野 千秋, 谷野 孝徳, 田中 勉, 福田 秀樹, 近藤 昭彦

  4th Korea-Japan Workshop on Combinatorial Bioengineering, Nov. 2008, English, Association of Combinatorial Bioengineering, Kookmin University, Seoul, International conference

  Poster presentation


• Efficient and direct ethanol production from raw starch by d-integrant polyploid fusants of Saccharomyces cerevisiae

  山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  4th Korea-Japan Workshop on Combinatorial Bioengineering, Nov. 2008, English, Association of Combinatorial Bioengineering, Seoul, Korea, International conference

  Poster presentation


• Biorefinery strategy by cell surface engineered yeast strains

  荻野 千秋, 近藤 昭彦

  4th Korea-Japan Workshop on Combinatorial Bioengineering, Nov. 2008, English, Association of Combinatorial Bioengineering, Seoul, Korea, International conference

  Poster presentation


• 特異性改変型バイオナノカプセルを用いたバイオイメージング及びDDSへの応用

  近藤 昭彦, 宍戸 卓矢, 田中 勉, 荻野 千秋

  第17回日本バイオイメージング学会, Oct. 2008, Japanese, 日本バイオイメージング学会, 千葉市, Domestic conference

  Oral presentation


• Improvement of Yeast Xylose Uptake Ability for Efficient Bioethanol Production

  TANINO Takanori, OGINO Chiaki, KUMAGAI Norihisa, OHMURA Naoto

  International Workshop on Process Intensification 2008, Oct. 2008, English, 化学工学会粒子流体プロセス部会, 化学工学会システム・情報・シミュレーション部会, 化学工学会反応工学部会, 化学工学会マイクロ化学研究会, Tokyo Institute of Technology, International conference

  Poster presentation


• セルラーゼ発現酵母によるセルロースからのバイオエタノール生産

  柳瀬 修平, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  化学工学会 第40回 秋季大会, Sep. 2008, Japanese, 化学工学会, 宮城県仙台市, Domestic conference

  Poster presentation


• 無蒸煮デンプンから効率的にエタノールを生産する高機能酵母の創製

  山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  第60回 日本生物工学会2008年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conference

  Oral presentation


• 遺伝子組み換え放線菌を用いたセルラーゼ発現システムの構築

  伊藤 裕子, 宮下 徹, 清水 宣明, 荻野 千秋

  第60回 日本生物工学会2010年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conference

  Oral presentation


• リン脂質代謝酵素のイオン液体中での反応解析

  宮下 徹, 伊藤 裕子, 清水 宣明, 荻野 千秋

  第60回 日本生物工学会2009年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conference

  Oral presentation


• キチン結合ドメインを用いた麹菌細胞表層提示技術の開発

  田淵 聡一郎, 伊藤 純二, 足立 崇, 石田 博樹, 秦 洋二, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  第60回 日本生物工学会2008年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conference

  Oral presentation


• Streptomyces griseocarneus NBRC13471由来スフィンゴミエリナーゼC の遺伝子クローニングと発現

  杉森 大助, 冨田 優, 荻野 千秋, 劉 暁麗

  第60回 日本生物工学会2008年度大会, Aug. 2008, Japanese, 日本生物工学会, 宮城県仙台市, Domestic conference

  Oral presentation


• Oxidative-stress-induced metabolite production with sonocatalytic formation of reactive oxygen species

  KATSUDA Tomohisa, OGINO Chiaki, IWASAKI Daiki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki

  American Society for Microbiology 108th General Meeting, Jun. 2008, English, American Society for Microbiology, Boston, USA, International conference

  Poster presentation


• フローサイトメーター(FCM)を利用した酵母GPCRシグナリング解析技術

  Ishii Jun, Tsutomu Tanaka, Matsumura Shizuka, Tatematsu Kenji, Kuroda Shun'ichi, Ogino Chiaki, Fukuda Hideki, Kondo Akihiko

  化学工学会第73年会, Mar. 2008, Japanese, 化学工学会, 浜松市, Domestic conference

  Oral presentation


• 酵母GPCRシグナリングにおけるフローサイトメトリー解析技術

  Ishii Jun, Tsutomu Tanaka, Matsumura Shizuka, Tatematsu Kenji, Kuroda Shun'ichi, Ogino Chiaki, Fukuda Hideki, Kondo Akihiko

  第6回最先端バイオテクノロジー若手発表会, Feb. 2008, Japanese, 化学工学会関西支部化学工学会バイオ部会, 西宮市, Domestic conference

  Poster presentation


• ストレス応答を利用したHaematococcus pluvialisによるアスタキサンチン生産の高効率化

  SENGA Itaru, YAMAGAMI Keisuke, KATSUDA Tomohisa, OGINO Chiaki, SHIMIZU Nobuaki, KATOH Shigeo

  第6回 最先端バイオテクノロジー公開セミナー, Feb. 2008, Japanese, 化学工学会関西支部、化学工学会バイオ部会, 西宮, Domestic conference

  Oral presentation


• Ultrasonic facilitation of OH radical generation in the presence of TiO2

  SHIMIZU N, OGINO Chiaki, DADJOUR M.F

  ISSS2007, Dec. 2007, English, ソノケミストリー学会, 京都, International conference

  Oral presentation


• The cancer cell injury by combination of protein displayed titanium dioxide nanoparticle and ultrasound irradiation

  SASAI R, SONOKE S, OGINO Chiaki, KURODA S, SHIMIZU N

  ISSS2007, Dec. 2007, English, ソノケミストリー学会, 京都, International conference

  Oral presentation


• Construction of biomolecule-immobilized TiO2 nanoparticle for applying to new cell injuring method with ultrasound irradiation

  OGINO Chiaki, SASAI R, SONOKE S, KURODA S, SHIMIZU N

  ISSS2007, Dec. 2007, English, ソノケミストリー学会, 京都, International conference

  Oral presentation


• Analysis of Sonodynamic Sterilization of Escherichia coli at the Surface of TiO2

  RAHMAN M.M, OGINO Chiaki, SHIMIZU N

  ISSS2007, Dec. 2007, English, ソノケミストリー学会, 京都, International conference

  Oral presentation


• Analysis of H2O2 formation induced by ultrasonic irradiation with TiO2. K

  SAKIYAMA K, ONIWA T, OGINO Chiaki, SHIMIZU N

  ISSS2007, Dec. 2007, English, ソノケミストリー学会, 京都, International conference

  Oral presentation


• Oxidative treatment of H. pluvialis with the sonocatalytic reaction

  KATSUDA Tomohisa, OGINO Chiaki, IWASAKI Hiroki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki

  YABEC2007, Oct. 2007, English, Young Asian Biochemical Engineers Community, Seoul, Korea, International conference

  Poster presentation


• Application of biomolecule-immobilized TiO2 nanoparticle for chemical compound degradation and mammalian cell killing

  OGINO Chiaki, SASAI R, SONOKE S, SHIMIZU N

  YABEC2007, Oct. 2007, English, YABEC, Souel 韓国, International conference

  Oral presentation


• 放線菌を宿主とするリン脂質代謝酵素の変異体解析

  伊藤 吉基, 宮下 徹, OGINO Chiaki, 清水 宣明

  日本生物工学会第59回大会, Sep. 2007, Japanese, 日本生物工学会, 東広島, Domestic conference

  Oral presentation


• 放線菌Streptomyces lividans における分泌シグナルのタンパク生産に及ぼす影響解析

  吉野 慶和, 伊藤 裕子, OGINO Chiaki, 清水 宣明

  日本生物工学会第59回大会, Sep. 2007, Japanese, 日本生物工学会, 東広島, Domestic conference

  Oral presentation


• 二酸化チタン-超音波励起法を利用したヘマトコッカス藻によるアスタキサンチン生産の誘導

  KATSUDA Tomohisa, OGINO Chiaki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki

  第59回生物工学会大会, Sep. 2007, Japanese, 日本生物工学会, 広島, Domestic conference

  Oral presentation


• Over-expression system of secretory phospholipase D by Streptomyces lividans

  OGINO Chiaki, KUBO T, YOSHINO K, SHIMIZU N

  ECB13, Sep. 2007, English, ECB, バルセロナ スペイン, International conference

  Oral presentation


• New SELEX strategy for screening of DNA aptamer by AFM.

  OGINO Chiaki, MIYACHI Y, SHIMIZU N

  The 2nd International Workshop on Approaches to Single-Cell Analysis, Sep. 2007, English, 東京, International conference

  Oral presentation


• Induction of astaxantin biosynthesis in H. pluvialis using TiO2 and ultrasounds

  KATSUDA Tomohisa, OGINO Chiaki, YAMAGAMI K, KATOH S, SHIMIZU N

  ECB13, Sep. 2007, English, ECB, バルセロナ スペイン, International conference

  Oral presentation


• Induction of astaxanthin biosynthesis in H. pluvialis using TiO2 and ultrasounds

  KATSUDA Tomohisa, OGINO Chiaki, YAMAGAMI Keisuke, KATOH Shigeo, SHIMIZU Nobuaki

  13th European Congress on Biotechnology, Sep. 2007, English, European Federation of Biotechnology, Barcelona, Spain, International conference

  Poster presentation


• Phospholipid conversion in ionic liquid by phospholipase D

  OGINO Chiaki, MIYASHITA T, SHIMIZU N

  2nd International Congress on Ionic Liquids (COIL-2), Aug. 2007, English, 横浜, International conference

  Oral presentation


• 放線菌の新規タンパク質発現系構築に関する研究

  久保 孝文, 吉野 和慶, 伊藤 吉基, 宮下 徹, OGINO Chiaki, 清水 宣明

  化学工学会第72年会, Mar. 2007, Japanese, 化学工学会, 京都, Domestic conference

  Oral presentation


• 組織ターゲティング機能を有する二酸化チタンナノ粒子によるがん細胞障害

  柴田 直範, 笹井 良祐, 高木 圭子, 黒田 俊一, OGINO Chiaki, 清水 宣明

  化学工学会第72年会, Mar. 2007, Japanese, 化学工学会, 京都, Domestic conference

  Oral presentation


• リン脂質代謝酵素の酵素活性に及ぼすイオン液体の効果

  OGINO Chiaki, 伊藤 吉基, 宮下 徹, 清水 宣明

  日本化学会第87春季年会, Mar. 2007, Japanese, 日本化学会, 吹田, Domestic conference

  Oral presentation


• 8OH-dGを認識するDNAアプタマーの機能評価

  宮地 佑典, 伊藤 裕子, OGINO Chiaki, 清水 宣明

  日本化学会第87春季年会, Mar. 2007, Japanese, 日本化学会, 吹田, Domestic conference

  Oral presentation


• Structural analysis of 8OH-dG DNA aptamer

  OGINO Chiaki, MIYACHI Y, ITOU Y, SHIMIZU N

  9th Japan-China-Korea Joint symposium on Enzyme Engineering, Nov. 2006, English, 酵素工学研究会, 大津, International conference

  Oral presentation


• Over-expression system for secretory enzyme by Streptomyces lividans

  OGINO Chiaki, KUBO T, YOSHINO K, ITOU Y, MIYASHITA T, SHIMIZU N

  YABEC2006, Nov. 2006, English, YABEC, 高雄, International conference

  Oral presentation


• Effect of solvent and ionic liquid on phospholipid conversion in bi-phase system by phospholipase D

  ITOU Y, KUBO T, YOSHINO K, MIYASHITA T, OGINO Chiaki, SHIMIZU N

  9th Japan-China-Korea Joint symposium on Enzyme Engineering, Nov. 2006, English, 酵素工学研究会, 大津, International conference

  Oral presentation


• Construction of secretional expression system by Streptomyces lividans

  YOSHINO T, KUBO T, ITOU Y, MIYASHITA T, OGINO Chiaki, SHIMIZU N

  9th Japan-China-Korea Joint symposium on Enzyme Engineering, Nov. 2006, English, 酵素工学研究会, 大津, International conference

  Oral presentation


• 二酸化チタン超音波触媒法を応用した有害微生物殺菌システム

  MIZANUR R.M, OGINO Chiaki, 清水 宣明

  第15回ソノケミストリー討論会, Oct. 2006, Japanese, ソノケミストリー学会, 金沢, Domestic conference

  Oral presentation


• 二酸化チタン超音波触媒法による細胞死誘導

  高木 圭子, OGINO Chiaki, 清水 宣明

  第15回ソノケミストリー討論会, Oct. 2006, Japanese, ソノケミストリー学会, 金沢, Domestic conference

  Oral presentation


• 二酸化チタン・超音波法によるOHラジカルの生成と溶存アルゴンの促進効果

  藤平 敦司, OGINO Chiaki, 清水 宣明

  第15回ソノケミストリー討論会, Oct. 2006, Japanese, ソノケミストリー学会, 金沢, Domestic conference

  Oral presentation


• 二酸化チタンへの超音波照射による過酸化水素生成機構の解析

  崎山 和亨, OGINO Chiaki, 清水 宣明

  第15回ソノケミストリー討論会, Oct. 2006, Japanese, ソノケミストリー学会, 金沢, Domestic conference

  Oral presentation


• 放線菌を用いたホスホリパーゼD酵素の遺伝子組み換え生産の培養特性解析に関する研究

  久保 孝文, 吉野 和慶, 伊藤 吉基, 宮下 徹, OGINO Chiaki, 清水 宣明

  日本生物工学会第58回大会, Sep. 2006, Japanese, 日本生物工学会, 豊中, Domestic conference

  Oral presentation


• 二酸化チタン・超音波法によるOHラジカルの生成と溶存アルゴンの促進効果

  藤平 敦司, 崎山 和享, OGINO Chiaki, 清水 宣明

  化学工学会第38回秋季大会, Sep. 2006, Japanese, 化学工学会, 福岡, Domestic conference

  Oral presentation


• 二酸化チタン・超音波触媒法によるがん細胞障害と増殖抑制

  高木 圭子, OGINO Chiaki, 清水 宣明

  日本生物工学会第58回大会, Sep. 2006, Japanese, 日本生物工学会, 豊中, Domestic conference

  Oral presentation


• 組織認識能を持つ二酸化チタン内包リポソームの創製

  OGINO Chiaki, 高木 圭子, 清水 宣明

  日本生物工学会第58回大会, Sep. 2006, Japanese, 日本生物工学会, 豊中, Domestic conference

  Oral presentation


• 生体ストレスマーカー分子8OH-dGを認識するDNAアプタマーに関する研究

  宮地 佑典, 伊藤 裕子, OGINO Chiaki, 清水 宣明

  日本生物工学会第58回大会, Sep. 2006, Japanese, 日本生物工学会, 豊中, Domestic conference

  Oral presentation


• 8OH-dGを認識するDNAアプタマーに関する基礎研究

  宮地 祐介, OGINO Chiaki, 清水 宣明

  化学工学会第38回秋季大会, Sep. 2006, Japanese, 化学工学会, 福岡, Domestic conference

  Oral presentation


• 二酸化チタン・超音波触媒法によるがん細胞傷害と増殖抑制

  山岸 紗弥花, 高木 圭子, FARSHBAF M.D, OGINO Chiaki, 清水 宣明

  化学工学会第71年会, Mar. 2006, Japanese, 化学工学会, 東京, Domestic conference

  Oral presentation


• 組織認識能をもつ二酸化チタン内包リポソームの創製

  野瀬 律子, 高木 圭子, FARSHBAF M.D, 中川 晋作, OGINO Chiaki, 清水 宣明

  化学工学会第71年会, Mar. 2006, Japanese, 化学工学会, 東京, Domestic conference

  Oral presentation


• リン脂質代謝酵素の蓋構造変化における活性相関に関する研究(II)

  大石 多佳子, 伊藤 吉基, 高田 奈充子, OGINO Chiaki, 清水 宣明

  化学工学会第71年会, Mar. 2006, Japanese, 化学工学会, 東京, Domestic conference

  Oral presentation


• 放線菌Streptomyces cinnamoneum由来発現プロモーターを用いたタンパク質発現系構築に関する研究

  久保 孝文, 吉野 和慶, OGINO Chiaki, 清水 宣明

  日本生物工学会第57回大会, Nov. 2005, Japanese, 日本生物工学会, 筑波, Domestic conference

  Oral presentation


• リン脂質代謝酵素ホスホリパーゼDの蓋構造の活性相関に関する研究

  大石 多佳子, 高田 奈充子, 伊藤 吉基, OGINO Chiaki, 清水 宣明

  日本生物工学会第57回大会, Nov. 2005, Japanese, 日本生物工学会, 筑波, Domestic conference

  Oral presentation


• 有害微生物の超音波殺菌に対する二酸化チタンの増強効果

  FARSHBAF D.M, OGINO Chiaki, 清水 宣明

  第14回ソノケミストリー討論会, Oct. 2005, Japanese, ソノケミストリー学会, 豊橋, Domestic conference

  Oral presentation


• 放線菌を宿主とするタンパク質発現系の構築

  福本将士, OGINO Chiaki, 清水宣明

  化学工学会第70年会, Mar. 2005, Japanese, 化学工学会, 名古屋, Domestic conference

  Oral presentation


• リン脂質代謝酵素の蓋構造変化における活性相関に関する研究

  泉 麻里子, OGINO Chiaki, 清水 宣明

  化学工学会第70年会, Mar. 2005, Japanese, 化学工学会, 名古屋, Domestic conference

  Oral presentation


• Screening of DNA aptamer that recognizes nucleotide derivative

  OGINO Chiaki, MURATA T, MIYACHI Y, SHIMIZU N

  Bioseparation2004, Oct. 2004, English, 化学工学会, 福岡, International conference

  Oral presentation


• Investigation of the Catalytic Function of Phospholipase D by Site Directed Mutagenesis

  OGINO Chiaki, IZUMI M, OHISHI T, FUKUMOTO M, SHIMIZU N

  10thAPCChE, Oct. 2004, English, 化学工学会, 北九州, International conference

  Oral presentation


• Cell Disinfection by Ultrasonic Irradiation with TiO2 Photocatalyst

  FARSHBAF D.M, OGINO Chiaki, MATSUMURA S, NAKAMURA S, SHIMIZU N

  10thAPCChE, Oct. 2004, English, 化学工学会, 北九州, International conference

  Oral presentation


• 低分子化合物を認識するDNAアプタマーの取得とそのバイオセンサーへの応用

  OGINO Chiaki, 村田知之, 宮地佑典, 清水宣明

  化学工学会山口下関大会, Sep. 2004, Japanese, 化学工学会, 下関, Domestic conference

  Oral presentation


• 生理活性物質を認識するDNAアプタマーの探索とその機能評価

  OGINO Chiaki, 村田知之, 清水宣明

  日本生物工学会第56回大会, Sep. 2004, Japanese, 日本生物工学会, 名古屋, Domestic conference

  Oral presentation


• Screening of DNA aptamer that recognizes nucleotide derivative

  OGINO Chiaki, MURATA T, MIYACHI Y, SHIMIZU N

  YABEC2004, Sep. 2004, English, YABEC, 大阪, International conference

  Oral presentation


• 有機溶媒感受性プロモーターを用いた水質評価法の構築

  伊藤 武男, OGINO Chiaki, 森本 真壽, 清水 宣明

  化学工学会第69年会, Apr. 2004, Japanese, 化学工学会, 堺, Domestic conference

  Oral presentation


• Investigation of catalytic function of phospholipase D enzyme by site directed mutagenesis

  OGINO Chiaki, IZUMI M, OISHI T, SHIMIZU N

  1st PRICPS, Apr. 2004, English, 日本蛋白質学会, 横浜, International conference

  Oral presentation


• Disinfection of Legionella by sonocatalytic irradiation with TiO2 photocatalyst

  FARSHBAF D.M, 松村享, OGINO Chiaki, 清水宣明

  化学工学会第69年会, Apr. 2004, English, 化学工学会, 堺, Domestic conference

  Oral presentation


• 新規ラジカル生成法の環境浄化と医療への応用

  清水 宣明, OGINO Chiaki

  化学工学会京都大会, Dec. 2003, Japanese, 化学工学会, 京都, Domestic conference

  Oral presentation


• Screening of DNA aptamer that recognizes phenol molecule.

  OGINO Chiaki, MURATA T, MIYAMOTO N, KANEHIRA K, SHIMIZU N

  YABEC2003, Nov. 2003, English, YABEC, 済洲島, International conference

  Oral presentation


• 二酸化チタン・超音波照射法による大腸菌殺菌メカニズムの解析

  FARSHBAF D.M, 松村 享, OGINO Chiaki, 清水 宣明

  第12回ソノケミストリー討論会, Oct. 2003, Japanese, ソノケミストリー学会, 福岡, Domestic conference

  Oral presentation


• 放線菌由来リン脂質代謝酵素の変異体の機能解析

  OGINO Chiaki, 泉 麻里子, 大石 多佳子, 清水 宣明

  日本生物工学会第55回大会, Sep. 2003, Japanese, 日本生物工学会, 熊本, Domestic conference

  Oral presentation


• Investigation of catalytic function of phospholipase D enzyme by site directed mutagenesis

  OGINO Chiaki, IZUMI M, SHIMIZU N

  European Conference of Biotechnology (ECB) 11th, Aug. 2003, English, ECB, バーゼル・スイス, International conference

  Oral presentation


• 分子進化法を用いたPLDの触媒機能変換

  畑中 祐樹, 泉 麻里子, OGINO Chiaki, 清水 宣明

  化学工学会第68年会, Mar. 2003, Japanese, 化学工学会, 東京, Domestic conference

  Oral presentation


• 機能性DNA分子の探索と水質評価への応用

  金平 幸輝, 村田 知之, OGINO Chiaki, 清水 宣明

  化学工学会第68年会, Mar. 2003, Japanese, 化学工学会, 東京, Domestic conference

  Oral presentation


• Bio-sensing of benzene derivatives using Ps promoter gene and GFP as a reporter protein (II)

  OGINO Chiaki, IKENO S, ITO T, SHIMIZU N

  YABEC2002, Nov. 2002, English, YABEC, 台北, International conference

  Oral presentation


• 有機溶媒感受性プロモーターを用いた水質評価法の構築

  池野 慎也, 伊藤 武男, 杉野 康弘, OGINO Chiaki, 清水 宣明

  日本生物工学会第54回大会, Oct. 2002, Japanese, 日本生物工学会, 大阪, Domestic conference

  Oral presentation


• リン脂質代謝酵素ホスホリパーゼDの遺伝子工学的手法による触媒機能解析

  OGINO Chiaki, 畑中 祐樹, 泉 麻里子, 清水 宣明

  日本生物工学会第54回大会, Oct. 2002, Japanese, 日本生物工学会, 大阪, Domestic conference

  Oral presentation


• 有機化合物認識遺伝子群を用いた水質評価法の構築

  池野 慎也, 伊藤 武男, OGINO Chiaki, 川西 琢也, 林 良茂, 清水 宣明

  化学工学会第35回秋季大会, Sep. 2002, Japanese, 化学工学会, 神戸, Domestic conference

  Oral presentation


• 変異型ホスホリパーゼDを用いた新規リン脂質合成への検討

  畑中 祐樹, OGINO Chiaki, 川西 琢也, 林 良茂, 清水 宣明

  化学工学会第35回秋季大会, Sep. 2002, Japanese, 化学工学会, 神戸, Domestic conference

  Oral presentation


• 二酸化チタン界面での超音波力学的殺菌メカニズムの解析

  中村 淳, OGINO Chiaki, 川西 琢也, 林 良茂, 清水 宣明

  化学工学会第35回秋季大会, Sep. 2002, Japanese, 化学工学会, 神戸, Domestic conference

  Oral presentation


• 二酸化チタン・超音波法による内分泌撹乱物質の分解処理に関する研究

  前川 和人, 川西 琢也, 林 良茂, OGINO Chiaki, 清水 宣明

  化学工学会第35回秋季大会, Sep. 2002, Japanese, 化学工学会, 神戸, Domestic conference

  Oral presentation


• 有機溶媒感受性プロモーターを用いた水質評価法の構築

  池野 慎也, 伊藤 武男, OGINO Chiaki, 川西 琢也, 林 良茂, 清水 宣明

  化学工学会第67年会, Mar. 2002, Japanese, 化学工学会, 福岡, Domestic conference

  Oral presentation


• 二酸化チタン超音波照射法によるヒドロキシラジカルの生成と内分泌撹乱物質の分解処理

  島田 泰行, OGINO Chiaki, 川西 琢也, 林 良茂, 清水 宣明

  化学工学会第67年会, Mar. 2002, Japanese, 化学工学会, 福岡, Domestic conference

  Oral presentation


• ホスホリパーゼDにおける構造変化とリン脂質合成相関に関する解析

  OGINO Chiaki, 大村 有香, 畑中 祐樹, 川西 琢也, 林 良茂, 清水 宣明

  化学工学会第67年会, Mar. 2002, Japanese, 化学工学会, 福岡, Domestic conference

  Oral presentation


• Construction of functional phospholipid synthesis system by using mutant phospholipase D enzyme

  OGINO Chiaki, OHMURA Y, HATANAKA Y, KISHI N, SHIMIZU N

  YABEC2001, Oct. 2001, English, YABEC, 上海, International conference

  Oral presentation


• Construction of benzene derivatives biosensor using Ps promoter gene and GFP as a reporter gene

  IKENO S, OGINO Chiaki, ITO T, SHIMIZU N

  YABEC2001, Oct. 2001, English, YABEC, 上海, International conference

  Oral presentation


• ホスホリパーゼDの触媒機構解析と新規リン脂質合成への応用

  OGINO Chiaki, 大村 有香, 畑中 祐樹, 川西 琢也, 林 良茂, 清水 宣明

  化学工学会福井大会, Jul. 2001, Japanese, 化学工学会, 福井, Domestic conference

  Oral presentation


• レポーター遺伝子組換え微生物を用いる水質評価法の構築

  池野 慎也, OGINO Chiaki, 川西 琢也, 林 良茂, 清水 宣明

  化学工学会第66年会, Apr. 2001, Japanese, 化学工学会, 東広島, Domestic conference

  Oral presentation


• リン脂質代謝酵素ホスホリパーゼD の触媒機構及び活性化機構の解析

  大村 有香, 畑中 祐樹, OGINO Chiaki, 川西 琢也, 林 良茂, 清水 宣明

  化学工学会第66年会, Apr. 2001, Japanese, 化学工学会, 東広島, Domestic conference

  Oral presentation


• Improved catalytic properties of phospholipase D by site directed mutagenesis

  OGINO Chiaki, OMURA Y, KONDO Akihiko, SHIMIZU N, FUKUDA Hideki

  YABEC2000, Nov. 2000, English, YABEC, 福岡, International conference

  Oral presentation


• Secretory Overexpression of Phospholipase D By Recombinant Streptomyces lividans

  KANEMASU M, OGINO Chiaki, DAIDO H, HAYASHI Y, KONDO Akihiko, FUKUDA Hideki, KURODA S

  化学工学会秋季大会, Sep. 2000, English, 化学工学会, 浜松, Domestic conference

  Oral presentation


• 遺伝子工学的手法による活性変異型ホスホリパーゼDの構築と解析

  OGINO Chiaki, 大村 有香, 清水 宣明, 金升 将征, KONDO Akihiko, FUKUDA Hideki

  日本生物工学会第52回大会, Aug. 2000, Japanese, 日本生物工学会, 札幌, Domestic conference

  Oral presentation


• 放線菌Stv. cinnamoneum由来PLDの発現系構築と触媒機構解析

  大同 英則, OGINO Chiaki, 金升 将征, KONDO Akihiko, FUKUDA Hideki

  日本生物工学会第51回大会, Sep. 1999, Japanese, 日本生物工学会, 吹田市, Domestic conference

  Oral presentation