Research activity information

• Multifunctional chemical inhibitors of the florigen activation complex discovered by structure-based high-throughput screening.

  Ken-Ichiro Taoka, Ikumi Kawahara, Shoko Shinya, Ken-Ichi Harada, Eiki Yamashita, Zenpei Shimatani, Kyoko Furuita, Tomoaki Muranaka, Tokitaka Oyama, Rie Terada, Atsushi Nakagawa, Toshimichi Fujiwara, Hiroyuki Tsuji, Chojiro Kojima

  Structure-based high-throughput screening of chemical compounds that target protein-protein interactions (PPI) is a promising technology for gaining insight into how plant development is regulated, leading to many potential agricultural applications. At present, there are no examples of using high-throughput screening to identify chemicals that target plant transcriptional complexes, some of which are responsible for regulating multiple physiological functions. Florigen, a protein encoded by FLOWERING LOCUS T (FT), was initially identified as molecule that promotes flowering and has since been shown to regulate flowering and other developmental phenomena such as tuber formation in potato. FT functions as a component of the florigen activation complex (FAC) with a scaffold protein 14-3-3 and FD, a bZIP transcription factor that activates downstream gene expression. Although 14-3-3 is an important component of FAC, there are little functional analysis of the 14-3-3 itself. Here, we report the results of a high-throughput in vitro fluorescence resonance energy transfer (FRET) screening of chemical libraries that enabled us to identify small molecules capable of inhibiting FAC formation. These molecules abrogate the in vitro interaction between 14-3-3 and OsFD1 peptide, a rice FD, by directly binding to 14-3-3. Treatment with S4, a specific hit molecule, strongly inhibited FAC activity and flowering in duckweed, tuber formation in potato and branching in rice in a dose-dependent manner. Our results demonstrate that the high-throughput screening approach based on three-dimensional structure of PPI is possible in plants. In this study, we have proposed good candidate compounds for future modification to obtain inhibitors of florigen-dependent processes through inhibition of FAC formation.

  Oct. 2022, The Plant journal : for cell and molecular biology, 112(6) (6), 1337 - 1349, English, International magazine

  Scientific journal


• Crystal structure of potato 14-3-3 protein St14f revealed the importance of helix I in StFDL1 recognition.

  Ken-Ichi Harada, Kyoko Furuita, Eiki Yamashita, Ken-Ichiro Taoka, Hiroyuki Tsuji, Toshimichi Fujiwara, Atsushi Nakagawa, Chojiro Kojima

  In potato (Solanum tuberosum L.), 14-3-3 protein forms a protein complex with the FLOWERING LOCUS T (FT)-like protein StSP6A and the FD-like protein StFDL1 to activate potato tuber formation. Eleven 14-3-3 isoforms were reported in potato, designated as St14a-k. In this study, the crystal structure of the free form of St14f was determined at 2.5 Å resolution. Three chains were included in the asymmetric unit of the St14f free form crystal, and the structural deviation among the three chain structures was found on the C-terminal helix H and I. The St14f free form structure in solution was also investigated by nuclear magnetic resonance (NMR) residual dipolar coupling analysis, and the chain B in the crystal structure was consistent with NMR data. Compared to other crystal structures, St14f helix I exhibited a different conformation with larger B-factor values. Larger B-factor values on helix I were also found in the 14-3-3 free form structure with higher solvent contents. The mutation in St14f Helix I stabilized the complex with StFDL1. These data clearly showed that the flexibility of helix I of 14-3-3 protein plays an important role in the recognition of target protein.

  Jul. 2022, Scientific reports, 12(1) (1), 11596 - 11596, English, International magazine

  Scientific journal


• Novel assays to monitor gene expression and protein-protein interactions in rice using the bioluminescent protein, NanoLuc

  Ken-ichiro Taoka, Zenpei Shimatani, Koji Yamaguchi, Mana Ogawa, Hiromi Saitoh, Yoichi Ikeda, Hiroko Akashi, Rie Terada, Tsutomu Kawasaki, Hiroyuki Tsuji

  Mar. 2021, PLANT BIOTECHNOLOGY, 38(1) (1), 89 - 99, English

  Scientific journal


• Characterization of Frond and Flower Development and Identification of FT and FD Genes From Duckweed Lemna aequinoctialis Nd.

  Akiko Yoshida, Ken-Ichiro Taoka, Aoi Hosaka, Keisuke Tanaka, Hisato Kobayashi, Tomoaki Muranaka, Kiminori Toyooka, Tokitaka Oyama, Hiroyuki Tsuji

  Duckweeds (Araceae: Lemnoideae) are aquatic monocotyledonous plants that are characterized by their small size, rapid growth, and wide distribution. Developmental processes regulating the formation of their small leaf-like structures, called fronds, and tiny flowers are not well characterized. In many plant species, flowering is promoted by the florigen activation complex, whose major components are florigen FLOWERING LOCUS T (FT) protein and transcription factor FD protein. How this complex is regulated at the molecular level during duckweed flowering is also not well understood. In this study, we characterized the course of developmental changes during frond development and flower formation in Lemna aequinoctialis Nd, a short-day plant. Detailed observations of frond and flower development revealed that cell proliferation in the early stages of frond development is active as can be seen in the separate regions corresponding to two budding pouches in the proximal region of the mother frond. L. aequinoctialis produces two stamens of different lengths with the longer stamen growing more rapidly. Using high-throughput RNA sequencing (RNA-seq) and de novo assembly of transcripts from plants induced to flower, we identified the L. aequinoctialis FT and FD genes, whose products in other angiosperms form a transcriptional complex to promote flowering. We characterized the protein-protein interaction of duckweed FT and FD in yeast and examined the functions of the two gene products by overexpression in Arabidopsis. We found that L. aequinoctialis FTL1 promotes flowering, whereas FTL2 suppresses flowering.

  2021, Frontiers in plant science, 12, 697206 - 697206, English, International magazine

  Scientific journal


• TFL1-Like Proteins in Rice Antagonize Rice FT-Like Protein in Inflorescence Development by Competition for Complex Formation with 14-3-3 and FD

  Miho Kaneko-Suzuki, Rie Kurihara-Ishikawa, Chiaki Okushita-Terakawa, Chojiro Kojima, Misa Nagano-Fujiwara, Izuru Ohki, Hiroyuki Tsuji, Ko Shimamoto, Ken-Ichiro Taoka

  Oxford University Press, Mar. 2018, Plant and Cell Physiology, 59(3) (3), 458 - 468, English

  [Refereed]

  Scientific journal


• Inheritance of co-edited genes by CRISPR-based targeted nucleotide substitutions in rice

  Zenpei Shimatani, Ushio Fujikura, Hisaki Ishii, Yusuke Matsui, Minoru Suzuki, Yuki Ueke, Ken-ichiro Taoka, Rie Terada, Keiji Nishida, Akihiko Kondo

  Elsevier Masson SAS, 2018, Plant Physiology and Biochemistry, 131, 78 - 83, English

  [Refereed]

  Scientific journal


• Potato Tuber Induction is Regulated by Interactions Between Components of a Tuberigen Complex

  Chin-Jit Teo, Kenta Takahashi, Kanae Shimizu, Ko Shimamoto, Ken-ichiro Taoka

  Feb. 2017, PLANT AND CELL PHYSIOLOGY, 58(2) (2), 365 - 374, English

  [Refereed]

  Scientific journal


• フロリゲン受容体の発見とその後

  辻寛之, 田岡健一郎

  Apr. 2016, 化学と生物, 54, 358 - 364, Japanese

  [Refereed]

  Scientific journal


• Hd3a promotes lateral branching in rice

  Hiroyuki Tsuji, Chika Tachibana, Shojiro Tamaki, Ken-ichiro Taoka, Junko Kyozuka, Ko Shimamoto

  Apr. 2015, PLANT JOURNAL, 82(2) (2), 256 - 266, English

  [Refereed]

  Scientific journal


• Florigen signaling

  Hiroyuki Tsuji, Ken-ichiro Taoka

  Academic Press, 2014, Enzymes, 35, 113 - 144, English

  [Refereed]

  Scientific journal


• Florigen signaling.

  Tsuji H, Taoka K

  2014, The Enzymes, 35, 113 - 144

  [Refereed]


• Structure and function of florigen and the receptor complex

  Ken-ichiro Taoka, Izuru Ohki, Hiroyuki Tsuji, Chojiro Kojima, Ko Shimamoto

  May 2013, TRENDS IN PLANT SCIENCE, 18(5) (5), 287 - 294, English

  [Refereed]

  Scientific journal


• Florigen in rice: complex gene network for florigen transcription, florigen activation complex, and multiple functions

  Hiroyuki Tsuji, Ken-ichiro Taoka, Ko Shimamoto

  May 2013, CURRENT OPINION IN PLANT BIOLOGY, 16(2) (2), 228 - 235, English

  [Refereed]

  Scientific journal


• 花成ホルモン“フロリゲン”の構造と機能

  辻寛之, 田岡健一郎

  Mar. 2013, ライフサイエンス領域融合レビューLEADING AUTHOR’S, Japanese

  Scientific journal


• Functional Diversification of FD Transcription Factors in Rice, Components of Florigen Activation Complexes

  Hiroyuki Tsuji, Hiroyuki Nakamura, Ken-ichiro Taoka, Ko Shimamoto

  Mar. 2013, PLANT AND CELL PHYSIOLOGY, 54(3) (3), 385 - 397, English

  [Refereed]

  Scientific journal


• 花成ホルモンフロリゲンとその受容体の構造解析からみえてきたフロリゲン機能の分子基盤

  田岡健一郎, 大木出, 辻寛之, 児嶋長次郎, 島本功

  Sep. 2012, 化学と生物, 50, 654 - 659, Japanese

  Scientific journal


• 花咲かホルモン（フロリゲン）

  辻寛之, 田岡健一郎

  Aug. 2012, 植調, 46, 11 - 18, Japanese

  Scientific journal


• 14-3-3タンパク質はフロリゲンの細胞内における受容体としてはたらく

  田岡健一郎, 大木出, 辻寛之, 児嶋長次郎, 島本功

  Sep. 2011, ライフサイエンス新着論文レビュー FIRST AUTHOR’S, Japanese

  Scientific journal


• 14-3-3 proteins act as intracellular receptors for rice Hd3a florigen

  Ken-ichiro Taoka, Izuru Ohki, Hiroyuki Tsuji, Kyoko Furuita, Kokoro Hayashi, Tomoko Yanase, Midori Yamaguchi, Chika Nakashima, Yekti Asih Purwestri, Shojiro Tamaki, Yuka Ogaki, Chihiro Shimada, Atsushi Nakagawa, Chojiro Kojima, Ko Shimamoto

  Aug. 2011, NATURE, 476(7360) (7360), 332 - U97, English

  [Refereed]

  Scientific journal


• Regulation of flowering in rice: two florigen genes, a complex gene network, and natural variation

  Hiroyuki Tsuji, Ken-ichiro Taoka, Ko Shimamoto

  Feb. 2011, CURRENT OPINION IN PLANT BIOLOGY, 14(1) (1), 45 - 52, English

  [Refereed]

  Scientific journal


• 融合発展する構造生物学とケミカルバイオロジーの最前線

  田岡健一郎, 辻寛之, 島本功

  Sep. 2009, 蛋白質核酸酵素 臨時増刊, 54(12) (12), 1702 - 1707, Japanese

  Scientific journal


• Reciprocal phosphorylation and glycosylation recognition motifs control NCAPP1 interaction with pumpkin phloem proteins and their cell-to-cell movement

  Ken-ichiro Taoka, Byung-Kook Ham, Beatriz Xoconostle-Cazares, Maria R. Rojas, William J. Lucas

  Jun. 2007, PLANT CELL, 19(6) (6), 1866 - 1884, English

  [Refereed]

  Scientific journal


• FLOWERING LOCUS T protein may act as the long-distance florigenic signal in the cucurbits

  Ming-Kuem Lin, Helene Belanger, Young-Jin Lee, Erika Varkonyi-Gasic, Ken-Ichiro Taoka, Eriko Miura, Beatriz Xoconostle-Cazares, Karla Gendler, Richard A. Jorgensene, Brett Phinney, Tony J. Lough, William J. Lucas

  May 2007, PLANT CELL, 19(5) (5), 1488 - 1506, English

  [Refereed]

  Scientific journal


• Arabidopsis CUP-SHAPED COTYLEDON3 regulates postembryonic shoot meristem and organ boundary formation

  Ken-ichiro Hibara, Md. Rezaul Karim, Shinobu Takada, Ken-ichiro Taoka, Masahiko Furutani, Mitsuhiro Aida, Masao Tasaka

  Nov. 2006, PLANT CELL, 18(11) (11), 2946 - 2957, English

  [Refereed]

  Scientific journal


• Plasmodesmal-associated protein kinase in tobacco and Arabidopsis recognizes a subset of non-cell-autonomous proteins

  JY Lee, K Taoka, BC Yoo, G Ben-Nissan, DJ Kim, WJ Lucas

  Oct. 2005, PLANT CELL, 17(10) (10), 2817 - 2831, English

  [Refereed]

  Scientific journal


• The NAC domain mediates functional specificity of CUP-SHAPED COTYLEDON proteins

  K Taoka, Y Yanagimoto, Y Daimon, K Hibara, M Aida, M Tasaka

  Nov. 2004, PLANT JOURNAL, 40(4) (4), 462 - 473, English

  [Refereed]

  Scientific journal


• FASCIATA genes for chromatin assembly factor-1 in Arabidopsis maintain the cellular organization of apical meristems

  H Kaya, K Shibahara, K Taoka, M Iwabuchi, B Stillman, T Araki

  Jan. 2001, CELL, 104(1) (1), 131 - 142, English

  [Refereed]

  Scientific journal


• Regulation of histone gene expression during the cell cycle

  T Meshi, K Taoka, M Iwabuchi

  Aug. 2000, PLANT MOLECULAR BIOLOGY, 43(5-6) (5-6), 643 - 657, English

  [Refereed]

  Scientific journal


• Identification of three kinds of mutually related composite elements conferring S phase-specific transcriptional activation

  K Taoka, H Kaya, T Nakayama, T Araki, T Meshi, M Iwabuchi

  Jun. 1999, PLANT JOURNAL, 18(6) (6), 611 - 623, English

  [Refereed]

  Scientific journal


• S phase-specific expression of plant histone genes

  T Meshi, K Taoka, M Iwabuchi

  Jun. 1998, JOURNAL OF PLANT RESEARCH, 111(1102) (1102), 247 - 251, English

  [Refereed]

  Scientific journal


• The modular structure and function of the wheat H1 promoter with S phase-specific activity

  K Taoka, N Ohtsubo, Y Fujimoto, K Mikami, T Meshi, M Iwabuchi

  Mar. 1998, PLANT AND CELL PHYSIOLOGY, 39(3) (3), 294 - 306, English

  [Refereed]

  Scientific journal


• STRUCTURAL AND FUNCTIONAL-CHARACTERIZATION OF 2 WHEAT HISTONE H2B PROMOTERS

  P YANG, KI TAOKA, T NAKAYAMA, M IWABUCHI

  Apr. 1995, PLANT MOLECULAR BIOLOGY, 28(1) (1), 155 - 172, English

  [Refereed]

  Scientific journal

• Regulatory mechanism of the condensate formation induced by liquid-liquid phase separation of florigen activation complex

  ENOMOTO Mayu, ANZAWA Suai, KOIZUMI Yuka, FURUITA Kyoko, TAOKA Ken-ichiro, TAOKA Ken-ichiro, NISHIDA Keiji, KONDO Akihiko, KODAMA Takashi, FUJIWARA Toshimichi, TSUJI Hiroyuki, TSUJI Hiroyuki, KOJIMA Chojiro, KOJIMA Chojiro

  2023, 日本植物生理学会年会(Web), 64th


• The LLPS formation by flowering activation complex and flowering regulating mechanism

  ENOMOTO Mayu, ANZAWA Suai, KOIZUMI Yuka, FURUITA Kyoko, TAOKA Kenichiro, TAOKA Kenichiro, NISHIDA Keiji, KONDO Akihiko, KODAMA Takashi, FUJIWARA Toshimichi, TSUJI Hiroyuki, TSUJI Hiroyuki, KOJIMA Chojiro, KOJIMA Chojiro

  2023, 生物物理(Web), 63(Supplement 1-2) (Supplement 1-2)


• イネ花成ホルモン蛋白質複合体によるLLPS形成と花成制御機構

  榎本麻由, 安澤すあい, 小泉優香, 古板恭子, 田岡健一郎, 田岡健一郎, 西田敬二, 近藤昭彦, 児玉高志, 藤原敏道, 辻寛之, 辻寛之, 児嶋長次郎, 児嶋長次郎

  2023, 日本蛋白質科学会年会プログラム・要旨集, 23rd (CD-ROM)


• Liquid-liquid phase separation of rice flowering hormone protein complex and elucidation of flowering regulating mechanism

  榎本麻由, 安澤すあい, 小泉優香, 田岡健一郎, 児玉高志, 古板恭子, 藤原敏道, 辻寛之, 辻寛之, 児嶋長次郎, 児嶋長次郎

  2022, 日本分子生物学会年会プログラム・要旨集(Web), 45th


• C-terminal intrinsically disordered region of flowering hormone protein Hd3a regulates flowering through liquid-liquid phase separation

  小泉優香, 安澤すあい, 田岡健一郎, 古板恭子, 藤原敏道, 辻寛之, 児嶋長次郎, 児嶋長次郎

  2021, 日本分子生物学会年会プログラム・要旨集(Web), 44th


• イネ花成ホルモン蛋白質の液-液相分離

  榎本麻由, 安澤すあい, 小泉優香, 田岡健一郎, 児玉高志, 白木賢太郎, 藤原敏道, 辻寛之, 児嶋長次郎, 児嶋長次郎

  2021, 日本蛋白質科学会年会プログラム・要旨集, 21st


• 多様な花成ホルモン蛋白質の構造・機能解析

  安澤すあい, 新家粧子, 古板恭子, 小篭蒼, 田岡健一郎, 辻寛之, 藤原敏道, 児嶋長次郎

  2019, 日本分子生物学会年会プログラム・要旨集(Web), 42nd


• 花成ホルモン蛋白質のNMR解析および¹⁹F-NMRスクリーニング

  安澤すあい, 新家粧子, 古板恭子, 小篭蒼, 田岡健一郎, 辻寛之, 藤原敏道, 児嶋長次郎, 児嶋長次郎

  2019, Abstracts. Annual Meeting of the NMR Society of Japan, 58th (CD-ROM)


• ジャガイモの14-3-3タンパク質St14fの結晶構造解析

  原田健一, 新家粧子, 山下栄樹, 田岡健一郎, 辻寛之, 藤原敏道, 中川敦史, 児嶋長次郎, 児嶋長次郎

  2018, 日本蛋白質科学会年会プログラム・要旨集, 18th


• フロリゲン受容体のNMR解析

  中村歩美, 樋口佳恵, 新家粧子, 田岡健一郎, 辻寛之, 藤原敏道, 木川隆則, 児嶋長次郎, 児嶋長次郎

  2018, 日本分子生物学会年会プログラム・要旨集(Web), 41st


• フロリゲン複合体形成を制御する化合物の探索

  河原郁美, 田岡健一郎, 藤原敏道, 児嶋長次郎

  2015, 日本蛋白質科学会年会プログラム・要旨集, 15th


• 植物ゲノム編集技術としての遺伝子ターゲティング:技術の現状とイネ耐病性関連遺伝子OsRac1の恒常活性化改変

  SHIMATANI ZEMPEI, TERADA RIE, DANG THU THI, KAWANO YOJI, TSUJI HIROYUKI, TAOKA KEN'ICHIRO, SHIMAMOTO KO

  21 Mar. 2014, 育種学研究, 16, 124, Japanese


• 膜透過ペプチドを利用したイネフロリゲンタンパク質直接導入による人為的開花制御

  WASHIDA HARUHIKO, TSUJI HIROYUKI, OKI IZURU, TAOKA KEN'ICHIRO, SHIMAMOTO KO

  12 Oct. 2013, 育種学研究, 15, 91, Japanese


• フロリゲン活性化複合体の新規転写因子OsFD2の機能解析

  TSUJI HIROYUKI, NAKAMURA HIROYUKI, TAOKA KEN'ICHIRO, SHIMAMOTO KO

  12 Oct. 2013, 育種学研究, 15, 150, Japanese


• イネのフロリゲンHd3aと相互作用する転写因子OsFDの機能解析

  TSUJI HIROYUKI, NAKAMURA HIROYUKI, TAMAKI SHOJIRO, TAOKA KEN'ICHIRO, SHIMAMOTO KO

  14 Mar. 2013, 日本植物生理学会年会要旨集, 54th, 214, Japanese


• イネTFL1ホモログRCNによる花成抑制の分子機構の解析

  TAOKA KEN'ICHIRO, TERAKAWA CHIAKI, OKI IZURU, TSUJI HIROYUKI, KOJIMA CHOJIRO, SHIMAMOTO KO

  14 Mar. 2013, 日本植物生理学会年会要旨集, 54th, 215, Japanese


• 植物が花を咲かせる分子メカニズムの解明を目指して―NMR分光学から構造生命科学へ―

  TAOKA KEN'ICHIRO, OKI IZURU, TSUJI HIROYUKI, SHIMAMOTO KO, KOJIMA CHOJIRO

  2013, 日本分光学会テラヘルツ分光部会シンポジウム講演要旨集, 2013, 24 - 29, Japanese


• 花成ホルモン・フロリゲンの受容と機能のメカニズム

  TSUJI HIROYUKI, NAKASHIMA CHIKA, TAOKA KEN'ICHIRO, OKI IZURU, KOJIMA CHOJIRO, INADA NORIKO, SHIMAMOTO KO

  31 Aug. 2012, 日本遺伝学会大会プログラム・予稿集, 84th, 78, Japanese


• 膜透過ペプチドを利用した植物へのイネフロリゲンHd3aタンパク質直接導入

  WASHIDA HARUHIKO, TSUJI HIROYUKI, TAOKA KEN'ICHIRO, KOJIMA CHOJIRO, SHIMAMOTO KO

  29 Mar. 2012, 育種学研究, 14, 144, Japanese


• in vivo imaging of Florigen Activation Complex (FAC) comprising Hd3a, 14-3-3 and OsFD1 in rice

  TSUJI HIROYUKI, NAKASHIMA CHIKA, TAOKA KEN'ICHIRO, OKI IZURU, KOJIMA CHOJIRO, INADA NORIKO, SHIMAMOTO KO

  09 Mar. 2012, 日本植物生理学会年会要旨集, 53rd, 101, Japanese


• Florigen Hd3a protein acts as a mobile branching signal in rice.

  TSUJI HIROYUKI, TACHIBANA CHINATSU, TAMAKI SHOJIRO, TAOKA KEN'ICHIRO, KYOZUKA JUNKO, SHIMAMOTO KO

  09 Mar. 2012, 日本植物生理学会年会要旨集, 53rd, 205, Japanese


• フロリゲン活性化複合体FACの活性調節機構の解析

  TAOKA KEN'ICHIRO, OKI IZURU, TSUJI HIROYUKI, KOJIMA CHOJIRO, SHIMAMOTO KO

  09 Mar. 2012, 日本植物生理学会年会要旨集, 53rd, 205, Japanese


• 花成ホルモン・フロリゲンの受容と機能のメカニズム

  TSUJI HIROYUKI, TAOKA KEN'ICHIRO, OKI IZURU, TAMAKI SHOJIRO, NAKASHIMA CHIKA, NAKAMURA HIROYUKI, INADA NORIKO, KOJIMA CHOJIRO, SHIMAMOTO KO

  2012, 日本分子生物学会年会プログラム・要旨集(Web), 35th, 2W7III-5 (WEB ONLY), Japanese


• 植物花成ホルモン(フロリゲン)受容体による花成制御機構の分子基盤

  OKI IZURU, FURUITA KYOKO, HAYASHI KOKORO, TAOKA KEN'ICHIRO, TSUJI HIROYUKI, NAKAGAWA ATSUSHI, SHIMAMOTO KO, KOJIMA CHOJIRO

  2012, 日本生化学会大会(Web), 85th, 3T08-04 (WEB ONLY), Japanese


• フロリゲンによる花成の分子機構と花成以外の形態形成制御

  SHIMAMOTO KO, TAOKA KEN'ICHIRO, TSUJI HIROYUKI, OKI IZURU, KOJIMA CHOJIRO

  2012, 日本生化学会大会(Web), 85th, 2S19-5 (WEB ONLY), Japanese


• Hd3a‐14‐3‐3‐OsFD1からなる複合体Florigen Activation Complexの機能解析

  TSUJI HIROYUKI, TAOKA KEN'ICHIRO, OKI IZURU, NAKASHIMA CHIKA, KOJIMA CHOJIRO, SHIMAMOTO KO

  23 Sep. 2011, 育種学研究, 13, 82, Japanese


• 植物花成ホルモンフロリゲンによる花成誘導メカニズムの分子基盤

  OKI IZURU, FURUITA KYOKO, HAYASHI KOKORO, TAOKA KEN'ICHIRO, TSUJI HIROYUKI, NAKAGAWA ATSUSHI, SHIMAMOTO KO, KOJIMA CHOJIRO

  27 May 2011, 日本蛋白質科学会年会プログラム・要旨集, 11th, 120, Japanese


• イネフロリゲンHd3aタンパク質複合体の機能解析

  TAOKA KEN'ICHIRO, SHIMADA CHIHIRO, YANASE TOMOKO, OKI IZURU, TSUJI HIROYUKI, KOJIMA CHOJIRO, SHIMAMOTO KO

  11 Mar. 2011, 日本植物生理学会年会要旨集, 52nd, 158, Japanese


• イネのフロリゲンHd3aと相互作用する転写因子OsFDの機能解析

  TSUJI HIROYUKI, TAMAKI SHOJIRO, TAOKA KEN'ICHIRO, SHIMAMOTO KO

  11 Mar. 2011, 日本植物生理学会年会要旨集, 52nd, 158, Japanese


• 植物花成ホルモン(フロリゲン)受容体による新たな花成誘導機構の分子基盤

  OKI IZURU, FURUITA KYOKO, HAYASHI KOKORO, TAOKA KEN'ICHIRO, TSUJI HIROYUKI, NAKAGAWA ATSUSHI, SHIMAMOTO KO, KOJIMA CHOJIRO

  2011, 日本分子生物学会年会プログラム・要旨集(Web), 34th, 2T5A-4 (WEB ONLY), Japanese


• 植物細胞内におけるFlorigen Activation Complex(FAC)の解析

  NAKASHIMA CHIKA, TSUJI HIROYUKI, TAOKA KEN'ICHIRO, INADA NORIKO, SHIMAMOTO KO

  2011, 日本分子生物学会年会プログラム・要旨集(Web), 34th, 4P-0303 (WEB ONLY), Japanese


• 遺伝子組み換えによらない植物細胞へのタンパク質直接導入

  WASHIDA HARUHIKO, TSUJI HIROYUKI, TAOKA KEN'ICHIRO, KOJIMA CHOJIRO, SHIMAMOTO KO

  24 Sep. 2010, 育種学研究, 12, 215, Japanese


• イネのフロリゲンHd3aと相互作用する転写因子OsFD1の機能解析

  TSUJI HIROYUKI, TAOKA KEN'ICHIRO, OKI IZURU, YAMAGUCHI MIDORI, OGAKI YUKA, PURWESTRI YEKTI ASIF, KOJIMA CHOJIRO, SHIMAMOTO KO

  24 Sep. 2010, 育種学研究, 12, 133, Japanese


• イネフロリゲンタンパク質Hd3aの花成制御機構の構造基盤

  OKI IZURU, FURUITA KYOKO, TAOKA KEN'ICHIRO, HAYASHI KOKORO, FUKADA HARUMI, TSUJI HIROYUKI, NAKAGAWA ATSUSHI, SHIMAMOTO KO, KOJIMA CHOJIRO

  15 May 2010, 日本蛋白質科学会年会プログラム・要旨集, 10th, 151, Japanese


• イネフロリゲンHd3aタンパク質の分子機能の構造的基盤

  OKI IZURU, HAYASHI KOKORO, FURUITA KYOKO, FUKADA HARUMI, TAOKA KEN'ICHIRO, TSUJI HIROYUKI, SHIMAMOTO KO, KOJIMA CHOJIRO

  12 Mar. 2010, 日本植物生理学会年会要旨集, 51st, 214, Japanese


• イネフロリゲンHd3aタンパク質と相互作用する転写因子OsFD1の機能解析

  TSUJI HIROYUKI, TAOKA KEN'ICHIRO, OKI IZURU, YAMAGUCHI MIDORI, OGAKI YUKA, KOJIMA CHOJIRO, SHIMAMOTO KO

  12 Mar. 2010, 日本植物生理学会年会要旨集, 51st, 214, Japanese


• イネフロリゲンHd3aタンパク質の機能ドメイン解析

  TAOKA KEN'ICHIRO, SHIMADA CHIHIRO, YANASE TOMOKO, OKI IZURU, TSUJI HIROYUKI, KOJIMA CHOJIRO, SHIMAMOTO KO

  12 Mar. 2010, 日本植物生理学会年会要旨集, 51st, 214, Japanese


• イネフロリゲンHd3aによる遺伝子発現制御機構の解析

  SHIMADA CHIHIRO, TAOKA KEN'ICHIRO, TSUJI HIROYUKI, SHIMAMOTO KO

  2010, 生化学, ROMBUNNO.2P-1071, Japanese


• イネのフロリゲンHd3aタンパク質と相互作用する転写因子OsKANADI1の機能解析

  UCHIMURA SHOHEI, TSUJI HIROYUKI, PURWESTRI YEKTI ASIH, YANASE TOMOKO, TAOKA KEN'ICHIRO, SHIMAMOTO KO

  2010, 生化学, ROMBUNNO.2P-1070, Japanese


• ターゲットタンパク研究プログラムの成果 花成ホルモン,フロリゲン その構造解析から分子機構の理解へ

  TAOKA KEN'ICHIRO, TSUJI HIROYUKI, SHIMAMOTO KO

  10 Sep. 2009, 蛋白質 核酸 酵素, 54(12) (12), 1702 - 1707, Japanese


• PVXベクターを用いたイネフロリゲンHd3aのタバコにおける機能評価系の確立

  MURAKAMI YUKIKO, TAOKA KEN'ICHIRO, TSUJI HIROYUKI, SHIMAMOTO KO

  2009, 日本分子生物学会年会講演要旨集, 32nd(Vol.4) (Vol.4), 179, Japanese


• イネのフロリゲンHd3aの相互作用する14‐3‐3タンパク質の解析

  YANASE TOMOKO, OGAKI YUKA, PURWESTRI YEKTI ASIH, TAOKA KEN'ICHIRO, WONG HANN LING, TSUJI HIROYUKI, SHIMAMOTO KO

  2008, 生化学, 3P-1238, Japanese


• NAC domain mediates functional specificity of CUP-SHAPED COTYLEDON 1 (CUC1) and CUP-SHAPED COTYLEDON 2 (CUC2): domain analysis of CUC1 and CUC2.

  Ken-ichiro Taoka, Yoshiko Yanagimoto, Yasufumi Daimon, Ken-ichiro Hibara, Mitsuhiro Aida, Masao Tasaka

  2004, The Plant Journal, 40, 462 - 473


• FUNCTIONAL ANALYSIS OF ARABIDOPSIS CUC1 AND CUC2 GENES :

  TAOKA Kenichiro, AIDA Mitsuhiro, TASAKA Masao

  Japanese Society of Plant Physiologists, 2001, Plant and cell physiology, 42, s43, English


• FASCIATA GENES REGULATE THE EXPRESSION OF WUSCHEL GENE IN SHOOT APICAL MERISTEM IN ARABIDOPSIS :

  KAYA Hidetaka, TAOKA Kenichiro, KOBAYASHI Yashushi, Tamai Hiroki, Aida Mitsuhiro, Tasaka Masao, MESHI Tetsuo, IWABUCHI Masaki, ARAKI Takashi

  Japanese Society of Plant Physiologists, 2000, Plant and cell physiology, 41, s208, English


• The role of FASCIATA genes that encode subunits of Chromatin Assembly Factor-I in apical meristem organization in Arabidopsis

  KAYA Hidetaka, TAOKA Ken-ichiro, KOBAYASHI Yasushi, MESHI Tetsuo, IWABUCHI Masaki, ARAKI Takashi

  Mar. 1999, Plant and cell physiology, 40, s16 - s16, English


• Meristematic tissue-specific gene expression in Arabidopsis conferred by three types of the Oct motif

  TAOKA Ken-ichiro, KAYA Hidetaka, ARAKI Takashi, MESHI Tetsuo, IWABUCHUI Masaki

  01 Dec. 1998, 日本分子生物学会年会プログラム・講演要旨集, 21, 432 - 432, Japanese


• Analysis of cis-regulatory region responsible for S phase-specific transcription of wheat histone H1 gene.

  田岡健一郎, 大坪憲弘, 岩淵雅樹

  Nov. 1995, 日本分子生物学会年会プログラム・講演要旨集, 18th, 474, Japanese


• コムギヒストンH1遺伝子の転写調節に関わるシス配列の同定

  田岡健一郎, 大坪憲弘, 三上浩司, 岩淵雅樹

  1994, 日本植物学会大会研究発表記録, 58th, 304, Japanese

• The Enzymes

  辻寛之, 田岡健一郎

  Joint work, Signaling pathways in Plants, Elsevier, Sep. 2014


• 植物ゲノム機能のダイナミズム―転写因子による発現制御

  田岡健一郎, 田坂昌生

  Joint work, シュプリンガーフェアラーク東京, Dec. 2001

• フロリゲン活性化複合体を阻害する化合物による花成調節

  田岡 健一郎, 河原 郁美, 新家 粧子, 島谷 善平, 寺田 理枝, 辻 寛之, 児嶋 長次郎

  植物化学調節学会第 55 回大会, Nov. 2020

  Oral presentation


• フロリゲン活性化複合体阻害化合物による花成調節

  田岡 健一郎, 河原 郁美, 島谷 善平, 寺田 理枝, 辻 寛之, 児嶋 長次郎

  第61回日本植物生理学会年会, Mar. 2020


• フロリゲン複合体形成を制御する化合物による花成調節

  田岡健一郎, 河原郁美, 島谷善平, 寺田理枝, 辻寛之, 児嶋長次郎

  第３７回日本植物細胞分子生物学会大会, Sep. 2019


• 高感度な発光レポーターNanoLucの植物細胞での利用

  田岡健一郎, 島谷善平, 小川真奈, 齋藤洋美, 池田洋一, 赤司裕子, 山口公志, 寺田理枝, 川崎努, 辻寛之

  第６０回日本植物生理学会年会, Mar. 2019


• RCN, rice TFL1, antagonize Hd3a in inflorescence development by competition for complex formation with 14-3-3 and FD

  K.Taoka, M. Kaneko-Suzuki, C. Okushita-Terakawa, C. Kojima, H. Tsuji, K.Shimamoto

  The 25th International Congress on Sexual Plant Reproduction, Jun. 2018


• Hd3a and RCN in rice antagonize in inflorescence development through competition for complex formation with 14-3-3 and FD

  田岡健一郎, 鈴木美穂, 辻寛之, 児嶋長次郎, 島本功

  2017 Cold Spring Harbor Asia Conference plant cell & developmental biology, May 2017


• フロリゲン複合体形成を制御する花成調節化合物の探索

  田岡健一郎, 河原郁美, 児嶋長次郎

  第３４回日本植物細胞分子生物学会大会, Sep. 2016


• ジャガイモ塊茎形成制御におけるTFL1ホモログの役割

  田岡健一郎, 高橋賢多, 齋藤亜美, 張禎日, 島本功

  第５７回日本植物生理学会年会, Mar. 2016


• ジャガイモ塊茎形成制御機構の解析

  田岡健一郎, 張禎日, 高橋賢多, 齋藤亜美, 清水かなえ, 島本功

  第１２９回日本育種学会大会, Mar. 2016


• チューベリゲンによるジャガイモ塊茎形成制御の分子機構

  田岡健一郎, 張禎日, 高橋賢多, 齋藤亜美, 清水かなえ, 島本功

  第１２回日本ナス科コンソーシアム年会, Sep. 2015


• チューベリゲンによるジャガイモ塊茎形成制御の分子機構

  田岡健一郎, 張禎日, 高橋賢多, 齋藤亜美, 清水かなえ, 島本功

  第１２８回日本育種学会大会, Sep. 2015


• FAC様チューベリゲン複合体によるジャガイモ塊茎形成制御機構の解析

  田岡健一郎, 高橋賢多, 齋藤亜美, 張禎日, 島本功

  第５６回日本植物生理学会年会, Mar. 2015


• 花成ホルモン（フロリゲン）が季節性の開花を制御するメカニズム

  田岡健一郎

  平成２６年度名城大学組換えDNA講演会, Nov. 2014

  [Invited]


• ジャガイモ塊茎形成におけるチューベリゲン複合体の機能解析

  田岡健一郎, 張禎日, 齋藤亜美, 清水かなえ, 島本功

  第５５回日本植物生理学会年会, Mar. 2014


• Analysis of florigen function in potato tuberization

  K.Taoka

  10th JSOL International Symposium on Solanaceae Genomics, Nov. 2013


• イネTFL1ホモログRCNによる花成抑制の分子機構の解析

  田岡健一郎, 寺川千晶, 大木出, 辻寛之, 児嶋長次郎, 島本功

  第５４回日本植物生理学会年会, Mar. 2013


• Analysis of Regulatory Mechanisms for Florigen Activation Complex

  田岡健一郎, 大木出, 辻寛之, 児嶋長次郎, 島本功

  The International Congress on Plant Molecular Biology (IPMB 2012), Oct. 2012


• フロリゲン活性化複合体FACの活性調節機構の解析

  田岡健一郎, 大木出, 辻寛之, 児嶋長次郎, 島本功

  第５３回日本植物生理学会年会, Mar. 2012


• イネフロリゲンHd3aタンパク質複合体の機能解析

  田岡健一郎, 島田千尋, 柳瀬朋子, 大木出, 辻寛之, 児嶋長次郎, 島本功

  第５２回日本植物生理学会年会, Mar. 2011


• イネフロリゲンHd3aタンパク質の機能ドメイン解析

  田岡健一郎, 島田千尋, 柳瀬朋子, 大木出, 辻寛之, 児嶋長次郎, 島本功

  イネ遺伝学・分子生物学ワークショップ2010, Jul. 2010


• イネフロリゲンHd3aタンパク質の機能ドメイン解析

  田岡健一郎, 島田千尋, 柳瀬朋子, 大木出, 辻寛之, 児嶋長次郎, 島本功

  第５１回日本植物生理学会年会, Mar. 2010


• 原形質連絡を介したタンパク質の細胞間移行制御における翻訳後修飾の役割

  田岡健一郎, Byung-Kook Ham, William J Lucas

  第７０回日本植物学会年会, Sep. 2006


• 原形質連絡を介した高分子の細胞間移行制御におけるNCAPP1及びタンパク質翻訳後修飾の役割

  田岡健一郎, Byung-Kook Ham, William J Lucas

  第２８回日本分子生物学会年会, Dec. 2005


• Molecular function of NCAPP1 in regulating macromolecular trafficking through plasmodesmata

  田岡健一郎, William J Lucas

  Plant Biology 2005, Jul. 2005


• Molecular analysis of Arabidopsis NCAPP1 homologs

  田岡健一郎, William J Lucas

  5th International Plasmodesmata meeting, Sep. 2004


• 茎頂分裂組織形成に関わるシロイヌナズナCUC1,CUC2遺伝子の機能解析

  田岡健一郎, 相田光宏, 田坂昌生

  第２４回日本分子生物学会年会, Dec. 2001


• シロイヌナズナCUC1,CUC2遺伝子の機能解析

  田岡健一郎, 相田光宏, 田坂昌生

  第４２回日本植物生理学会年会, Mar. 2001


• ３つのタイプのOct配列によるシロイヌナズナの分裂組織特異的な転写制御

  田岡健一郎, 賀屋秀隆, 荒木崇, 飯哲夫, 岩渕雅樹

  第２1回日本分子生物学会年会, Dec. 1998


• S期及び分裂組織特異的転写制御と３つのタイプのOct配列の機能

  田岡健一郎, 賀屋秀隆, 荒木崇, 飯哲夫, 岩渕雅樹

  第２０回日本分子生物学会年会, Dec. 1997


• 植物ヒストン遺伝子のS期特異的転写制御に関わるOct配列の存在意義

  田岡健一郎, 賀屋秀隆, 荒木崇, 飯哲夫, 岩渕雅樹

  第３８回日本植物生理学会年会, Sep. 1997


• 植物ヒストン遺伝子のS期及び分裂組織特異的転写制御に関わる３つのタイプのOct配列の役割

  田岡健一郎, 賀屋秀隆, 荒木崇, 飯哲夫, 岩渕雅樹

  第１５回日本植物細胞分子生物学会年会, Sep. 1997


• コムギヒストンH1遺伝子のS期特異的転写制御に関わるシス領域

  田岡健一郎, 大坪憲弘, 岩渕雅樹

  第１８回日本分子生物学会年会, Dec. 1995


• コムギヒストンH1遺伝子の転写調節に関わるシス配列の同定

  田岡健一郎, 大坪憲弘, 三上浩司, 岩渕雅樹

  第５８回日本植物学会大会, Sep. 1994


• コムギヒストンH1遺伝子の5’上流域にみられる特徴的な配列の機能解析

  田岡健一郎, 藤本宣充, 三上浩司, 岩渕雅樹

  第１５回日本分子生物学会年会, Dec. 1992


• コムギヒストンH1遺伝子の構造と発現制御

  田岡健一郎, 藤本宣充, 三上浩司, 南真樹, 川田健文, 岩渕雅樹

  第３３回日本植物生理学会年会, Mar. 1992

• 植物化学調節学会


• 日本育種学会


• 日本植物バイオテクノロジー学会


• 日本植物生理学会

• Analysis of tuberization by florigen activation complex

  TAOKA KEN-ICHIRO

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Yokohama City University, 01 Apr. 2017 - 31 Mar. 2020

  The molecular mechanism for day-length dependent tuberization of potato was analyzed. Here we used a potato cultivar, andigena W553-4, because Sayaka, which we used in the previous study, showed a moderate response to photoperiod for tuberization. W553-4 showed an ideal strict response, although it has a drawback that the transformation efficiency was low. A protoplast assay showed that TFL1 and a chemical inhibitor can inhibit SP6A activity.


• Molecular Function of Florigen Activation Complex

  Tsuji Hiroyuki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A), Grant-in-Aid for Scientific Research (A), Yokohama City University, 01 Apr. 2016 - 31 Mar. 2020

  Florigen (its molecular nature is the FT/Hd3a protein) is a strong determinant of the timing of flowering in plants. The major function of florigen is executed by forming a transcriptional complex called florigen activation complex. Florigen is a globular protein whose biochemical function cannot be clearly identified, and the overall picture of the direct transcriptional targets of the florigen activation complex is unknown. We attempted to visualize the process of florigen distribution in the shoot apical meristem and promotion of downstream gene expression. We found that florigen accumulates in different region of the SAM in different step of flowering. The mechanism of this change depends on the formation of the florigen activation complex.


• Multidimensional exploration of logics of plant sexual development and reproduction

  Araki Takashi, YAMATO Katsuyuki T., ENDO Motomu, NIWA Masaki, TAOKA Ken-ichiro, NIWA Masaki, HIGO Asuka, INOUE Keisuke, TOMITA Yuki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Kyoto University, 28 Jun. 2013 - 31 Mar. 2018

  In order to understand underlying logic of plant developmental process leading to successful sexual reproduction, (1) metabolic basis of regulation of floral transition (in Arabidopsis) and (2) whole process of sexual reproduction from the induction to fertilization and embryogenesis (in Marchantia) were studied. Main achievement includes elucidation of a regulatory pathway of flowering by potassium, elucidation of temporal aspect of florigen (FT protein) transport, identification of specific amino acid residues involved in FT transport, establishment of transcriptional framework for male sexual organ and gamete development, identification of factors involved in germline segregation, identification of factors involved in sperm cell differentiation, identification of factors involved in regulation of induction of sexual development in response to environmental stimuli, and novel findings on fertilization, early stages of embryogenesis and sporogenesis.


• Functional analysis of florigen complex in potato tuber formation

  TAOKA KEN-ICHIRO

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), 01 Apr. 2014 - 31 Mar. 2017

  In this research project, we revealed that potato tuber formation is regulated by a molecular mechanism similar to that of flowering regulation by florigen. In the regulation of photoperiodic flowering, FT, a florigen, induces flowering by making florigen activation complex (FAC) which is composed of FT, 14-3-3, and FD. StSP6A, a potato FT, has been shown to induce tuber formation (Navarro et al., 2011). To examine the molecular mechanism of tuber induction, we performed Y2H, BiFC, in vitro pull-down assay, and phenotypic analysis of transgenic potato plants. We revealed that StSP6A can make complex with potato 14-3-3 and StFDL1, a FD-like protein. Ectopic expression of StSP6A induced tuberization in a 14-3-3 dependent manner. Knock-down of StFDL1 significantly delayed tuberization. Considering them together, it was shown that StSP6A makes FAC-like complex termed Tuberigen Activation Complex (TAC) which is composed of StSP6A, 14-3-3, and StFDL1 to induce tuberization.


• 茎頂分裂組織の相転換制御ロジックの解明

  田岡 健一郎

  日本学術振興会, 科学研究費助成事業 新学術領域研究(研究領域提案型), 新学術領域研究(研究領域提案型), 01 Apr. 2014 - 31 Mar. 2016

  RCN1-3の３重RNAi植物の表現型解析から、一次枝こう数やえい花数の有意な減少やHd3a過剰発現体に似た花序形態形成の異常が観察されたが、花成の有意な遅延は観察されなかった。そこで、すべてのRCN遺伝子（RCN1-4）の4重RNAi植物を作製し、その表現型解析をおこなったところ、有意なえい花数の減少と花成促進が観察された。これは、花芽分化や花成時期決定において、RCNが冗長的にHd3a活性を抑制していることを示唆している。RCNとHd3aの複合体形成における競合関係について、競合的in vitro pulldown assayを行なったところ、４つのRCNの間で14-3-3に対する結合能に大きな差はなく、RCN3はHd3aより５倍程度強く14-3-3に結合しうることが明らかになった。フロリゲン様FTタンパク質（StSP6A）は、14-3-3依存的にジャガイモの栄養繁殖器官である塊茎の形成促進に働くことから、Hd3aとRCNによるイネ茎頂分裂組織の分化バランス制御と類似の分子機構が塊茎形成制御にも働いていると予想された。ジャガイモ形質転換体を用いたジャガイモRCNホモログの解析から、ジャガイモRCNホモログがイネ花成と同様に14-3-3依存的に塊茎形成を抑制していることが明らかになった。 茎頂分裂組織におけるRCNとHd3aの共局在解析や、GFP融合RCNとHd3aの維管束から茎頂分裂組織への選択・非選択輸送経路の解析から、フロリゲンとアンチフロリゲンの競合バランスによる生殖成長制御モデルの分子基盤が得られるだろう。


• Molecular Mechanism of Florigen Function and Application of Florigen to Crop Improvement

  SHIMAMOTO Ko, TAOKA Ken-ichiro, TERADA Rie, KOJIMA Chojiro, TSUJI Hiroyuki, OHKI Izuru, TAMAKI Shojiro

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Specially Promoted Research, Grant-in-Aid for Specially Promoted Research, Nara Institute of Science and Technology, 2012 - 2013

  Comprehensive analyses of florigen, a flowering hormone, were performed to elucidate the molecular function of florigen. Accumulation of rice florigen Hd3a at shoot apical meristem (SAM), details of the structure of florigen activation complex (FAC), complex formation and its structure of rice antiflorigen RCN, transcriptome and methylome of SAM, potato tuberization control by FAC, were revealed. The potential for improvement of biomass and crop yield by manipulation of florigen was examined.

• フロリゲン活性化複合体

  大木出, 田岡健一郎, 辻寛之, 児嶋長次郎, 島本功

  特願2012-505750, 17 Mar. 2011, 特許5842287, 27 Nov. 2015

  Patent right


• フロリゲンの導入方法

  辻 寛之, 島本 功, 田岡 健一郎, 鷲田治彦

  特願PCT/JP2013/ 53043, 08 Feb. 2013

  Patent right