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佐藤 幸治大学院人間発達環境学研究科 人間発達専攻准教授
研究者基本情報
■ 学位■ 研究ニュース
■ 研究キーワード
■ 研究分野
■ 委員歴
研究活動情報
■ 受賞- 2019年01月 神戸大学, 神戸大学優秀若手研究者賞
- 2019年01月 公益財団法人日本スポーツ協会, 秩父宮スポーツ医・科学賞 奨励賞
- 2014年07月 日本運動生理学会, 若手研究奨励賞日本国国内学会・会議・シンポジウム等の賞
- 2013年03月 ヤマハ発動機, 特別チャレンジャー賞日本国出版社・新聞社・財団等の賞
- 2010年08月 American Physiological Society, Travel Awardアメリカ合衆国国際学会・会議・シンポジウム等の賞
- Dioscorea esculenta increases sex steroid hormones in obesity and type 2 diabetes that impairs steroidogenesis. However, the effects of chronic D. esculenta intake on testicular sex steroid hormone concentrations and testicular function especially protection from oxidative stress and inflammation is still unclear. The study assessed the effects of chronic D. esculenta intake and exercise training on testicular function in rats with type 2 diabetes. Twenty-week-old Otsuka Long-Evans Tokushima Fatty rats were randomly assigned to the control, exercise training (running at 25 m/min for 1 h, 5 days/week), and D. esculenta intake (0.3 % with normal feed) groups (n = 8 per group). Long-Evans Tokushima Otsuka rats served as the healthy controls (n = 7). Eight weeks of D. esculenta intake or exercise training significantly increased plasma concentrations of dehydroepiandrosterone (DHEA), free testosterone and 5α-dihydrotestosterone (DHT). No significant increases were observed in DHEA, testosterone, and DHT concentrations in the testis. Androgen receptor (AR) expression was significantly increased by D. esculenta intake and exercise training. The expression of cathepsin L, which is involved in sperm modification, did not significantly change, whereas the expression of glutathione peroxidase 4 (GPx4), which protects sperms from oxidative stress, significantly increased in the D. esculenta group. Additionally, metabolic clearance rate (MCR), an index of insulin resistance, significantly correlated with testicular weight and GPx4 expression. These results suggest that improvement in MCR through D. esculenta intake suppresses testicular atrophy and enhances the ability of protection from oxidative stress and inflammation in rats with type 2 diabetes.2025年03月, The Journal of steroid biochemistry and molecular biology, 250, 106730 - 106730, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- The aim of the present study was to investigate the effects of Oncostatin M receptor (OSMR) subunit gp130 knockdown on insulin-stimulated glucose metabolism-related signaling pathways and glucose uptake in skeletal muscle cells. siRNA-mediated gp130 knockdown was conducted in C2C12 muscle cells, and insulin was added and incubated for 1 h. The cells were cultivated to analyze the mRNA levels of gp130, phosphorylation of STAT3, and glucose metabolism-regulated signaling pathways, and OSM levels in the culture medium were analyzed. The phosphorylation of STAT 3 was significantly decreased in gp130-/- cell. The insulin stimulation was significantly increased in both gp130-/- and gp130+/+ and the phosphorylation of IRS-1 Ser 1101 was significantly decreased in gp130-/-. PI3-kinase activity and Akt Thr 308 phosphorylation were significantly decreased in gp130-/-. The insulin-stimulated increase in glucose uptake rate was significantly attenuated in gp130-/-. In the culture medium, OSM levels were significantly lower in gp130+/+compared to gp130-/- cell. In conclusion, the knockdown of gp130 caused a decrease in STAT 3 phosphorylation and resulted in the attenuation of insulin-mediated glucose metabolism signaling in skeletal muscle cells. Thus, an excessive increase in extracellular OSM may induce blunted insulin action in skeletal muscle cells.2024年07月, Scientific reports, 14(1) (1), 17496 - 17496, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Background: The lesser yam (Dioscorea esculenta) is a phytochemical tuber that contains substantial amounts of diosgenin. Dioscorea esculenta is normally cultivated in tropical/sub-tropical regions such as African countries, and Okinawa in Japan. However, it remains unknown whether it can be successfully cultivated in cold regions within a plastic greenhouse. This study investigated the differences in nutritional values and diosgenin content between lesser yams cultivated in a sub-tropical climate (Okinawa) and those cultivated in a cold region (northern Kyoto) within a plastic greenhouse. Methods: The tubers were planted in March 2021 and harvested in January 2022. The temperature of the plastic greenhouse was 25.6 °C throughout the cultivation period. Additionally, the soil properties were potting soil with 8 % nitrogen potassium phosphate. Samples of dioscorea esculenta from both regions were used to produce 1 kg of sliced tubers from six tubers, were dried with the peel, then pulverized. The diosgenin content was analyzed for each sample, and nutritional ingredients such as moisture, carbohydrates, protein, fat, ash, sodium, and energy were measured. Results: Most of the analyzed nutritional ingredients demonstrated comparable values between plants cultivated in the sub-tropical region and within the plastic greenhouse in the cold region. The diosgenin content was 0.12 g/100 g for tubers cultivated in the sub-tropical region and 0.1 g/100 g for those cultivated in the cold region within a plastic greenhouse. Conclusion: Nutritional ingredients and diosgenin contents were comparable between the two growing conditions. Dioscorea esculenta can be cultivated in sub-tropical regions and in cold regions within plastic greenhouses.2024年06月, South African Journal of Botany, 169, 1 - 5研究論文（学術雑誌）
- Mounting evidence links Type 1 diabetes (T1D) with cognitive dysfunction, psychiatric disorders, and synaptic alterations; however, the underlying mechanism remains unclear. Numerous synaptic proteins and synaptic adhesion molecules (SAMs) that orchestrate synaptic formation, restructuring, and elimination are essential for proper brain function. Currently, it is unclear whether the pathogenesis of T1D is related to the expression of synaptic proteins and SAMs. Here, we investigated whether T1D mice exhibited altered synaptic protein and SAM expression in the hippocampus and cortex. We discovered that T1D mice exhibited partially decreased levels of excitatory and inhibitory synapse proteins and SAMs, such as neurexins, neuroligins, and synaptic cell adhesion molecules. We also found that compared to control mice, T1D mice showed a marginal decrease in body weight and a significant increase in plasma glycoalbumin levels (a hyperglycemia marker). These results provide novel molecular-level insights into synaptic dysfunction in mice with T1D.Wiley, 2023年04月, Physiological Reports, 11(8) (8), e15673, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Elsevier BV, 2022年11月, Consciousness and Cognition, 106, 103431 - 103431[査読有り]研究論文（学術雑誌）
- Background Diosgenin, extracted from Dioscorea esculenta, has been reported to decrease prostaglandin E2 (PGE2) levels and any other inflammatory cytokine in rodents. However, it is still unclear whether D. esculenta intake suppressed PGE2 production and menstrual pain and premenstrual syndrome (PMS) in younger female. Aim This study aims to investigate the effect of D. esculenta intake on PGE2 and cytochrome c oxidase subunit 2 (COX-2) levels and on menstrual pain and PMS in young women. This is a randomized, double-blind, placebo-controlled, crossover study. Methods Ten healthy young females were administered either a placebo or D. esculenta (300 mg/day) for 4 weeks, followed by a 4-week washout period. Fasting blood sample was taken from the fingertips on the second day of menstrual cycle began and obtained 24h before the last D. esculenta to avoid acute effects. Participants then switched treatments for 4 weeks as a second trial. Plasma PGE2 and COX-2 levels were measured before and after each trial. The visual analogue scale (VAS), McGill pain questionnaire (MPQ), and Daily Record of Severity of Problems (DRSP) were also evaluated. The study was set and conducted from 2019 to 2020. Results PGE2 and COX-2 levels significantly decreased after D. esculenta intake compared to placebo (p = 0.038, p = 0.042 each). The VAS and DRSP scores were also significantly lower after D. esculenta intake (p = 0.046, p = 0.035 each). Conclusion Four-week D. esculenta intake suppressed PGE2 and COX-2 levels resulting in an improvement in PMS symptoms and menstrual pain in young women.2022年10月, Nutrition and Health, 2601060221130889 - 2601060221130889, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- 2021年12月, Gynecological and Reproductive Endocrinology & Metabolism, 英語Effects of acute aerobic exercise and menstrual cycle on immune responses in young women[査読有り]研究論文（学術雑誌）
- OBJECTIVE: Sulforaphane is a phytochemical that is commonly found in broccoli and broccoli sprouts. However, whether chronic sulforaphane ingestion suppresses heavy resistance exercise-induced muscle damage parameters in humans remains unknown. Therefore, this study investigated the effects of oral chronic sulforaphane ingestion on heavy resistance exercise-induced muscle damage parameters. METHODS: The study had a randomized, double-blind, placebo-controlled, cross-over design. Ten healthy young men (age: 22.0 ± 0.3 y; body weight: 62.6 ± 2.4 kg; height: 171.0 ± 0.1 cm) were administered placebo or sulforaphane (30 mg/d) for 4 wk at the first trial, then after a 4-wk washout period, the participants were administered the opposite treatment for 4 wk at the second trial. The participants were subjected to heavy resistance exercise (bench press, 85% of one-repetition maximum for three times with eight repetitions) after each administration, and blood samples were collected before and at 30 min and 24 h after each exercise session. RESULTS: In this study, 4 wk of sulforaphane intake decreased plasma levels of creatine kinase, especially creatine kinase levels from 30 min to 24 h and baseline to 24 h. Moreover, the change in interleukin-6 levels significantly decreased from baseline to 30 min on prolonged intake of sulforaphane. CONCLUSIONS: Together, these findings suggest that the oral chronic intake of sulforaphane suppressed the heavy resistance exercise-induced increase in muscle damage parameter and expression of inflammatory cytokines. The chronic use of sulforaphane may be a novel therapeutic candidate for the prevention of muscle damage in athletes training daily with high-intensity exercise.2021年04月, Nutrition (Burbank, Los Angeles County, Calif.), 90, 111266 - 111266, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Substantial evidence has linked dehydroepiandrosterone (DHEA) levels to the anti-obesity and anti-diabetic effects of exercise. While 5'-adenosine monophosphate-activated protein kinase (AMPK) is a negative regulator of adipocyte differentiation and lipid accumulation, activation of mammalian target of rapamycin complex 1 (mTORC1), which is inhibited by AMPK, is required for adipocyte differentiation and positively regulates lipid accumulation. DHEA treatment activates the AMPK pathway in C2C12 myotubes. Hence, DHEA addition to preadipocytes and adipocytes might activate AMPK and inhibit mTORC1, resulting in the inhibition of adipogenesis and lipid accumulation. Therefore, we investigated the effect of DHEA on the AMPK pathway, mTORC1 activity, adipocyte differentiation, and lipid accumulation in 3T3-L1 cells. DHEA suppressed lipid accumulation and adipogenic marker expression during differentiation. It also activated AMPK signaling in preadipocytes and adipocytes and suppressed mTORC1 activity during differentiation. These results suggest that the activation of the AMPK pathway and inhibition of mTORC1 activity may mediate the anti-obesity effect of DHEA, providing novel molecular-level insights into its physiological functions.Elsevier BV, 2020年06月, Biochemical and Biophysical Research Communications, 528(3) (3), 614 - 620, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- There is little consensus regarding the impacts of physical activity and nutrient intake on bone mineral density (BMD) in subjects with high or low levels of arterial stiffness. This study was performed to investigate whether physical activity and nutrient intake are associated with BMD in middle-aged women with high levels of arterial stiffness. The study population consisted of middle-aged women aged 40-64 years (n = 22). BMD was assessed by dual-energy X-ray absorptiometry. Carotid-femoral pulse wave velocity (cf-PWV) was used as an indicator of arterial stiffness. Subjects were divided into two groups by median cf-PWV. Physical activity in free-living conditions was evaluated using a triaxial accelerometer. Nutrient intake was also measured using the brief-type self-administered diet history questionnaire. In the High-PWV group, BMD showed a significant negative correlation with age. Using a partial correlation model, BMD was associated with the number of steps and unsaturated fatty acid intake in the High-PWV group. These results suggest that BMD in middle-aged women with high levels of arterial stiffness may be associated with both the number of steps and nutritional intake. Recommendations of physical activity and nutritional intake for the prevention of osteopenia should include consideration of arterial stiffness.2020年03月, International journal of environmental research and public health, 17(5) (5), 英語, 国際誌[査読有り]研究論文（学術雑誌）
- This study examined whether satisfaction with social interactions and the number of people interacted with during walking groups is associated with affective responses among older adults. Twenty-six older adults were asked to participate in five walking group sessions. The participants walked together for 40-50 min. In every session, the participants reported their affective responses to walking (positive engagement, tranquility, and negative affect), their level of satisfaction with the social interactions experienced, and the number of people interacted with during the walk. The available data were from 107 person-sessions. Multilevel models revealed that, although a higher number of people interacted with was not significantly associated with improvements in any affective responses, higher satisfaction with the interactions was significantly associated with improvements in positive engagement at both the within- and between-person levels. This study found that higher satisfaction with the interactions was associated with desirable affective responses among older adults.2019年11月, Journal of aging and physical activity, 28(2) (2), 1 - 7, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- NEW FINDINGS: What is the central question of this study? Type 2 diabetes mellitus (T2DM) causes skeletal muscle atrophy; does it affect resistance training (RT)-mediated molecular adaptations and subsequent muscle hypertrophy? What is the main finding and its importance? Although skeletal muscle mass and regulation were not preserved under conditions of T2DM, the response of RT-induced skeletal muscle hypertrophy was not impaired in T2DM rat skeletal muscle. These findings suggest that the capacity of RT-mediated muscle mass gain is not diminished in the T2DM condition. ABSTRACT: Type 2 diabetes mellitus (T2DM) is known to cause skeletal muscle atrophy. However, it is not known whether T2DM affects resistance training (RT)-mediated molecular adaptations and subsequent muscle hypertrophy. Therefore, we investigated the effect of T2DM on response of skeletal muscle hypertrophy to chronic RT using a rat resistance exercise mimetic model. T2DM and healthy control rats were subjected to 18 bouts (3 times per week) of chronic RT on unilateral lower legs. RT significantly increased gastrocnemius muscle mass and myonuclei in both T2DM and healthy control rats to the same extent, even though T2DM caused muscle atrophy in the resting condition. Further, T2DM significantly reduced mechanistic target of rapamycin complex 1 (mTORC1) activity (phosphorylation of p70S6KThr389 and 4E-BP1Thr37/46 ) to insulin stimulation and the number of myonuclei in the untrained basal condition, but RT-mediated adaptations were not affected by T2DM. These findings suggested that although the skeletal muscle mass and regulation were not preserved under basal conditions of T2DM, the response of RT-induced skeletal muscle hypertrophy was not impaired in T2DM rat skeletal muscle.2019年10月, Experimental physiology, 104(10) (10), 1518 - 1531, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- (一社)日本体力医学会, 2019年04月, 体力科学, 68(2) (2), 171 - 171, 日本語動脈硬化指標を用いて分類した中年期女性の骨密度と身体活動および栄養摂取の関係
- ELSEVIER, 2019年04月, J Steroid Biochem Mol Biol, 英語[査読有り]研究論文（学術雑誌）
- Resistance training (RT) has been known to be effective in maintaining and improving bone strength, which is based on bone mineral density (BMD) and bone quality. However, it is not clear whether RT is effective in improving bone strength in patients with type-2 diabetes mellitus (T2DM), who have a high risk of fracture. Therefore, we tested the effects of a 6-week RT regimen using percutaneous electrical stimulation in T2DM model rats, male Otsuka Long-Evans Tokushima Fatty (OLETF), and its control, Long-Evans Tokushima Otsuka (LETO). After 6 weeks of RT, tibial BMD in RT legs was significantly higher than that in control (CON) legs in both groups. In diaphyseal cortical bone, bone area/tissue area, and cortical thickness was significantly increased in RT legs compared with CON legs in both groups. Cortical porosity was highly observed in OLETF compared with LETO, but RT improved cortical porosity in both groups. Interestingly, trabecular number, trabecular thickness and trabecular space as well as BMD and bone volume/tissue volume in proximal tibial metaphyseal trabecular bone were significantly improved in RT legs compared with CON legs in both groups. In contrast, connectivity density and structural model index were not affected by RT. These results indicate that the 6-week RT regimen effectively increased BMD and improved bone quality in T2DM model rats as well as control rats. Therefore, RT may have the potential to improve bone strength and reduce fracture risk, even in patients with T2DM.American Physiological Society, 2019年, Physiological Reports, 7(6) (6), e14046, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- 2018年10月, Nutrients, 10(10) (10), 1543, 英語Effect of Mixed Meal and Leucine Intake on Plasma Amino Acid Concentrations in Young Men[査読有り]研究論文（学術雑誌）
- 現在,我が国では,日本肥満学会により内臓脂肪型肥満の診断方法が設けられており,臍位(腰椎4番目-5番目:L4-L5)における内臓脂肪面積測定やウエスト周囲径測定がゴールドスタンダードとされている.しかし現在のところ,内臓脂肪蓄積の分布とメタボリックシンドロームリスク因子の関連を検討した研究は,これまでのところ極めて少ない.そこで本研究は,日本人中高齢女性を対象に磁気共鳴画像診断法でみた内臓脂肪面積の分布とメタボリックシンドロームリスク因子との関係について検討することを目的とした.本研究の被験者の内臓脂肪面積は,V-3(臍から3cm下位)からV10(臍から10cm上位)までの全ての部位においてObesity群とNormal群の間に有意な差が認められた(p<0.001).また,Normal群とObesity群の内臓脂肪面積の差の割合を検討したところ,上腹部ほど内臓脂肪面積が多かった.空腹時血糖は上腹部で全被験者とObesity群において有意な相関がみられたが(Total:r=0.44,Obesity:r=0.41,p<0.05),臍位では両者とも有意な相関はみられなかった.メタボリックシンドロームリスク因子保有数は全被験者およびNormal群の上腹部において有意な関連が認められた.脈波伝搬速度(足首-上腕)はNormal群の上腹部にのみ有意な関連があった(r=0.39,p<0.05).HDLコレステロールは全被験者(r=-0.35,p<0.05)とObesity群(r=-0.41,p<0.05)において上腹部で有意な負の相関がみられたが,臍位ではその関係はみられなかった.これらの結果から,先行的に日本人男性で示されてきたが,本研究において日本人中高齢女性においても,上腹部の内臓脂肪面積はメタボリックシンドロームリスク因子と有意に関連することが明らかとなった.(著者抄録)京都滋賀体育学会, 2018年08月, 京都滋賀体育学研究, 34, 10-18 - 19, 日本語[査読有り]
- 2018年07月, 京都滋賀体育学研究, 34, 20-28肥満ラットの有酸素性トレーニングによる動脈血管の apelin産生の増加は内皮型一酸化窒素合成酵素の活性に影響する[査読有り]
- American Physiological Society, 2018年02月, Am J Physiol Endocrinol Metab, 英語Resistance training recovers attenuated APPL1 expression and improves insulin-induced Akt signal activation in skeletal muscle of type 2 diabetic rats.[査読有り]研究論文（学術雑誌）
- Dehydroepiandrosterone (DHEA) is a precursor of sex steroid hormones and is converted to testosterone and estradiol. Normally, androgens and estrogens produced adrenal cortex, testis, and ovary; however, recent studies revealed androgens and estrogens are synthesized by peripheral tissues such as brain, skin, liver, kidney, bone, etc. We found skeletal muscles are also capable of synthesizing androgens and estrogens from DHEA. Circulating DHEA provides substrates required for conversion into potent androgens and estrogens in peripheral tissues. Sex steroid hormone administration has important roles: one is that the enhancement of protein synthesis and anabolism, resulting in muscle growth and increased muscle strength. The other is improvement of hyperglycemia through the activation of glucose signaling pathway in skeletal muscle as well as acceleration of muscle lipid metabolism that increase peroxisome proliferator-activated receptor alpha (PPARα) and PPAR delta (PPARδ). We introduce the effect of DHEA and sex steroid hormones administration on muscle glucose and lipid metabolisms as well as the effect of sex steroid hormone on the muscle hypertrophy.2018年, Vitamins and hormones, 108, 205 - 221, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- 2018年, トレーニング科学, 30(1) (1), 45 - 54閉経後サルコペニア女性を対象とした骨密度に対する軽負荷パワートレーニング効果の個体差に関連する要因の検討[査読有り]
- 2018年, Frontiers in Physiology, 英語Muscle Androgen Receptor Content but Not Systemic Hormones is Associated with Resistance Training-Induced Skeletal Muscle Hypertrophy in Healthy, Young Men[査読有り]研究論文（学術雑誌）
- Insulin stimulates skeletal muscle glucose uptake via activation of the protein kinase B/Akt (Akt) pathway. Recent studies suggest that insulin downregulates AMP-activated protein kinase (AMPK) activity via Ser485/491 phosphorylation of the AMPK alpha-subunit. Thus lower blood insulin concentrations may induce AMPK signal activation. Acute exercise is one method to stimulate AMPK activation; however, no study has examined the relationship between blood insulin levels and acute resistance exercise-induced AMPK pathway activation. Based on previous findings, we hypothesized that the acute resistance exercise-induced AMPK pathway activation would be augmented by disruptions in insulin secretion through a decrease in AMPK alpha Ser485/491 inhibitory phosphorylation. To test the hypothesis, 10-wk-old male Sprague-Dawley rats were administered the toxin streptozotocin (STZ; 55 mg/kg) to destroy the insulin secreting beta-cells. Three days postinjection, the right gastrocnemius muscle from STZ and control rats was subjected to resistance exercise by percutaneous electrical stimulation. Animals were killed 0, 1, or 3 h later; activation of the Akt/AMPK and downstream pathways in the muscle tissue was analyzed by Western blotting and real-time PCR. Notably, STZ rats showed a significant decrease in basal Akt and AMPK alpha Ser485/491 phosphorylation, but substantial exercise-induced increases in both AMPK alpha Thr172 and acetyl-CoA carboxylase (ACC) Ser79 phosphorylation were observed. Although no significant impact on resistance exercise-induced Akt pathway activation or glucose uptake was found, resistance exercise-induced peroxisome proliferator-activated receptor (PPAR)-gamma coactivator-1 alpha (PGC-1 alpha) gene expression was augmented by STZ treatment. Collectively, these data suggest that circulating insulin levels may regulate acute resistance exercise-induced AMPK pathway activation and AMPK-dependent gene expression relating to basal AMPK alpha Ser485/491 phosphorylation.American Physiological Society, 2017年08月, American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 313(2) (2), R110 - R119, 英語[査読有り]研究論文（学術雑誌）
- Previous studies have demonstrated that an acute bout of aerobic exercise induces a subsequent delayed onset of hypoglycemia among patients with type 1 diabetes. However, the mechanisms of exercise-induced hypoglycemia in type 1 diabetes are still unclear. Streptozotocin (STZ) was injected to 6-week-old male Wistar rats, and three days after STZ injection, animals were randomly assigned into 2 groups: STZ with insulin only (STZ) and STZ with insulin and exercise (STZ+EX). Normal Wistar rats with exercise were used as control (CON+EX). Insulin was intraperitoneally injected (0.5 U/kg) to both STZ groups (-0.5 h), and a bout of aerobic exercise (15 m/min for 30 min) was conducted at euglycemic conditions (0 h). Blood was collected at 0, 1, 3, and 5 h after exercise from the carotid artery. While the blood glucose level was stable during the post-exercise period (0-5 h) in the STZ and CON+EX groups, it decreased significantly only in the STZ+EX group at 3 h. Plasma glucagon, adrenalin, and noradrenalin levels significantly increased at 1 h in the STZ group, whereas significant hormonal responses were observed at 5 h in the STZ+EX group. In skeletal muscle glucose metabolism-related pathway, the level of glucose transporter-4 (GLUT-4) translocation was significantly higher at 1 h in the CON and STZ groups. However, in the STZ+EX group, these activations were maintained by 5 h, indicating a sustained glucose metabolism in the STZ+EX group. A single bout of aerobic exercise induced a delayed onset of hypoglycemia in STZ-treated rats. A prolonged enhancement of GLUT-4 translocation and delayed counter-regulatory hormone responses may have contributed to the induction of hypoglycemia.PUBLIC LIBRARY SCIENCE, 2017年06月, PLOS ONE, 12(6) (6), 英語[査読有り]研究論文（学術雑誌）
- Background: Age-related reduction in bone mineral density (BMD) is generally accelerated in women after menopause, and could be even more pronounced in individuals with sarcopenia. Light-load power training with a low number of repetitions would increase BMD, significantly reducing bone loss in individuals at risk of osteoporosis. This study investigated the effects of low-repetition, light-load power training on BMD in Japanese postmenopausal women with sarcopenia. Methods: The training group (n = 7) followed a progressive power training protocol that increased the load with a weighted vest, for two sessions per week, over the course of 6 weeks. The training exercise comprised five kinds of exercises (squats, front lunges, side lunges, calf raises, and toe raises), and each exercise contained eight sets of three repetitions with a 15-s rest between each set. The control group (n = 8) did not undergo any training intervention. We measured BMD, muscle strength, and anthropometric data. Results: Within-group changes in pelvis BMD and knee extensor strength were significantly greater in the training group than the control group (p = 0.029 and 0.030 for pelvis BMD and knee extensor strength, respectively). After low-repetition, light-load power training, we noted improvements in pelvis BMD (1.6%) and knee extensor strength (15.5%). No significant within-or between-group differences were observed for anthropometric data or forearm BMD. Conclusions: Six weeks of low-repetition, light-load power training improved pelvis BMD and knee extensor strength in postmenopausal women with sarcopenia. Since this training program does not require high-load exercise and is therefore easily implementable as daily exercise, it could be an effective form of exercise for sedentary adults at risk for osteoporosis who are fearful of heavy loads and/or training that could cause fatigue.BIOMED CENTRAL LTD, 2017年05月, BMC GERIATRICS, 17(1) (1), 102, 英語[査読有り]研究論文（学術雑誌）
- American Colleage of Sports Medicine, 2017年05月, Med Sci Sports Exerc, 英語A Mechanism Underlying Preventive Effect of High-Intensity Training on Colon Cancer.[査読有り]研究論文（学術雑誌）
- The effects of chronic Dioscorea esculenta administration and exercise training on muscle sex steroid hormone levels and insulin resistance in type 2 diabetes rats was assessed. Twenty-week-old male Otsuka Long Evans Tokushima Fatty (OLETF) rats were assigned randomly to the control, D. esculenta treatment, D. esculenta with 5-reductase inhibitor treatment, or the exercise training groups (running at 25 m/min for 1 h, 5 d/wk; n = 10 each group). Eight weeks of D. esculenta treatment or exercise training significantly attenuated the increase in plasma insulin and fasting glucose levels. Plasma and muscle concentrations of dehydroepiandrosterone and 5-dihydrotestosterone (DHT) and the expression of 5-reductase increased significantly in the D. esculenta-treated and exercise training groups, and both treatments led to the upregulation of glucose transporter-4 translocation with concomitant increases in PKB and PKC-/ phosphorylation. Furthermore, the glucose metabolic clearance rate, which represents insulin sensitivity, increased significantly in both the D. esculenta-treated and exercise training groups. These effects were suppressed by administration of the DHT synthetic inhibitor. Together, these findings suggest that the D. esculenta-induced increase in muscle sex steroid hormone levels helps decrease insulin resistance in type 2 diabetes.FEDERATION AMER SOC EXP BIOL, 2017年02月, FASEB JOURNAL, 31(2) (2), 793 - 801, 英語[査読有り]研究論文（学術雑誌）
- Dietary protein intake is critical for maintaining an optimal muscle mass, especially among older individuals. Although protein supplementation during resistance training (RT) has been shown to further augment training-induced muscle mass in older individuals, the impact of daily variations in protein intake on training-induced muscle mass has not been explored thus far. Therefore, this study aimed to investigate the relationship between the dietary protein and amino acid intake and RT-induced muscle hypertrophy among older individuals. Ten healthy older men (n=10 mean age56±62 y body weight (BW)=61.5±2.2 kg height=1.65±0.02 m) participated in progressive RT performed 3 times/wk for 12 wk. Body composition (using DXA) and nutritional assessments (using a 3-d dietary record) were performed before and after the training period. Leg lean mass (LLM) increased significantly (15.0±0.8 vs. 15.4±0.8 kg, p< 0.05) after RT, with no change in dietary nutrient intake. The average dietary protein intake was 1.62±0.11 g/kg BW/d, while essential amino acids was 600±51 mg/kg BW/d. Although the correlation between the increase in LLM and dietary protein intake was not significant, a significant correlation was found between the increase in LLM and dietary essential amino acid (EAA) intake. Furthermore, there were significant correlations between the increase in LLM and protein as well as EAA (especially leucine) intake at breakfast among subjects with suboptimal protein intake (p< 0.05). Our study findings indicate that dietary protein as well as EAA intake may be significant contributing factors in muscle hypertrophic response during RT among healthy older men.Center for Academic Publications Japan, 2017年, Journal of Nutritional Science and Vitaminology, 63(6) (6), 379 - 388, 英語[査読有り]研究論文（学術雑誌）
- Regular resistance exercise induces skeletal muscle hypertrophy and improvement of glycemic control in type 2 diabetes patients. Administration of dehydroepiandrosterone (DHEA), a sex steroid hormone precursor, increases 5 alpha-dihydrotestosterone (DHT) synthesis and is associated with improvements in fasting blood glucose level and skeletal muscle hypertrophy. Therefore, the aim of this study was to investigate whether increase in muscle DHT levels, induced by chronic resistance exercise, can contribute to skeletal muscle hypertrophy and concomitant improvement of muscular glucose metabolism in type 2 diabetic rats. Male 20-week-old type 2 diabetic rats (OLETF) were randomly divided into 3 groups: sedentary control, resistance training (3 times a week on alternate days for 8 weeks), or resistance training with continuous infusion of a 5 alpha-reductase inhibitor (n = 8 each group). Age-matched, healthy nondiabetic Long-Evans Tokushima Otsuka (LETO) rats (n = 8) were used as controls. The results indicated that OLETF rats showed significant decrease in muscular DHEA, free testosterone, DHT levels, and protein expression of steroidogenic enzymes, with loss of skeletal muscle mass and hyperglycemia, compared to that of LETO rats. However, 8-week resistance training in OLETF rats significantly increased the levels of muscle sex steroid hormones and protein expression of steroidogenic enzymes with a concomitant increase in skeletal muscle mass, improved fasting glucose level, and insulin sensitivity index. Moreover, resistance training accelerated glucose transporter-4 (GLUT-4) translocation and protein kinase B and C-zeta/lambda(IA phosphorylation. Administering the 5a-reductase inhibitor in resistance-trained OLETF rats resulted in suppression of the exercise induced effects on skeletal muscle mass, fasting glucose level, insulin sensitivity index, and GLUT-4 signaling, with a decline in muscular DHT levels. These findings suggest that resistance training-induced elevation of muscular DHT levels may contribute to improvement of hyperglycemia and skeletal muscle hypertrophy in type 2 diabetic rats.PUBLIC LIBRARY SCIENCE, 2016年11月, PLoS One, 11(11) (11), 英語[査読有り]研究論文（学術雑誌）
- The accumulation of intramyocellular lipid (IMCL) and extramyocellular lipid (EMCL) is associated with arterial stiffness in middle-aged and older adults. Habitual aerobic exercise induces the improvement of arterial stiffness with reduction in fat accumulation. However, the relationship between aerobic exercise-induced changes in muscular lipids and arterial stiffness remains unclear. The purpose of this study was to investigate whether habitual aerobic exercise-induced changes in IMCL and EMCL content would lead to an improvement of arterial stiffness. First, in a cross-sectional study, we investigated whether cardiorespiratory fitness level affects the association between IMCL or EMCL content and arterial stiffness in 60 middle-aged and older subjects (61.0 +/- 1.3 years). Second, in an intervention study, we examined whether aerobic exercise training-induced changes in IMCL and EMCL content are associated with a reduction in arterial stiffness in 18 middle-aged and older subjects (67.0 +/- 1.7 years). In the cross-sectional study, IMCL content was negatively correlated with brachial-ankle pulse wave velocity (baPWV) (r = -0.47, P<0.05), whereas EMCL content was positively correlated with baPWV (r = 0.48, P<0.05) in the low-fitness group, but was not correlated in the high-fitness group. Furthermore, 8-week aerobic exercise training in older adults increased IMCL content and reduced EMCL content. The training-induced change in baPWV was negatively correlated with training-induced changes in IMCL but was positively correlated with training-induced changes in EMCL. These findings suggest that aerobic exercise training-induced changes in IMCL and EMCL content may be related to a reduction in arterial stiffness in middle-aged and older adults.NATURE PUBLISHING GROUP, 2016年10月, JOURNAL OF HUMAN HYPERTENSION, 30(10) (10), 606 - 612, 英語[査読有り]研究論文（学術雑誌）
- Objective Habitual stretching exercise increases carotid arterial compliance, and acute stretching exercise increases arterial compliance in patients with myocardial infarction. However, it is not known whether this arterial adaptation is sustained after exercise. The aim of this study was to examine the effect of a single bout of stretching exercise on the time course of systemic, central, and peripheral arterial stiffness in healthy young subjects. Design Twenty-six healthy young men performed static stretching exercise involving the entire body (trunk, upper limb, and lower limb) for 40 mins. Pulse-wave velocity (PWV; an index of arterial stiffness), blood pressure, and heart rate were measured before and 0, 15, 30, and 60 mins after stretching exercise. Results Femoral-ankle PWV and brachial-ankle PWV were reduced relative to baseline 15 and 30 mins after acute stretching (P < 0.05); however, these arterial responses were not sustained for longer periods, and both PWV values returned to the baseline levels within 60 mins. By contrast, carotid-femoral PWV was unchanged. Conclusion These results suggest that chronic and sufficient repetition of muscle stretch stimulation may result in chronic high arterial compliance, although a single bout of stretch exercise acutely affects arterial compliance.LIPPINCOTT WILLIAMS & WILKINS, 2016年10月, AMERICAN JOURNAL OF PHYSICAL MEDICINE & REHABILITATION, 95(10) (10), 764 - 770, 英語[査読有り]研究論文（学術雑誌）
- Regular exercise improves aging-induced deterioration of arterial stiffness, and is associated with elevated production of pentraxin 3 (PTX3) and anti-inflammatory as well as anti-atherosclerotic effects. However, the time-dependent effect of exercise training on arterial stiffness and PTX3 production remains unclear. The purpose of this study was to investigate the time course of the association between the effects of training on the circulating PTX3 level and arterial stiffness in middle-aged and older adults. Thirty-two healthy Japanese subjects (66.2 +/- 1.3 year) were randomly divided into two groups: training (exercise intervention) and sedentary controls. Subjects in the training group completed 8 weeks of aerobic exercise training (60-70% peak oxygen uptake (VO2peak) for 45 min, 3 days per week); during the training period, we evaluated plasma PTX3 concentration and carotid-femoral pulse wave velocity (cfPWV) every 2 wk. cfPWV gradually declined over the 8-week training period, and was significantly reduced after 6 and 8 week of exercise intervention (P < 0.05). Plasma PTX3 level was significantly increased after 4 weeks of the intervention (P < 0.05). In addition, the exercise training-induced reduction in cfPWV was negatively correlated with the percent change in plasma PTX3 level after 6 week (r = -0.54, P < 0.05) and 8 weeks (r = -0.51, P < 0.05) of the intervention, but not correlated at 4 weeks. Plasma PTX3 level was elevated at the early stage of the exercise training intervention, and was subsequently associated with training-induced alteration of arterial stiffness in middle-aged and older adults.NATURE PUBLISHING GROUP, 2016年09月, JOURNAL OF HUMAN HYPERTENSION, 30(9) (9), 521 - 526, 英語[査読有り]研究論文（学術雑誌）
- Acute aerobic exercise (AE) is a major physiological stimulus for skeletal muscle glucose uptake through activation of 5′ AMP-activated protein kinase (AMPK). However, the regulation of glucose uptake by acute resistance exercise (RE) remains unclear. To investigate the intracellular regulation of glucose uptake after acute RE versus acute AE, male Sprague–Dawley rats were divided into three groups: RE, AE, or nonexercise control. After fasting for 12�h overnight, the right gastrocnemius muscle in the RE group was exercised at maximum isometric contraction via percutaneous electrical stimulation (3ï¿½× 10�sec, 5 sets). The AE group ran on a treadmill (25�m/min, 60�min). Muscle samples were taken 0, 1, and 3�h after completion of the exercises. AMPK, Ca2+/calmodulin-dependent protein kinase II, and TBC1D1 phosphorylation were increased immediately after both forms of exercise and returned to baseline levels by 3�h. Muscle IGF1 expression was increased by RE but not AE, and maintained until 3�h after RE. Additionally, Akt and AS160 phosphorylation were sustained for 3�h after RE, whereas they returned to baseline levels by 3�h after AE. Similarly, GLUT4 translocation remained elevated 3�h after RE, although it returned to the baseline level by 3�h after AE. Overall, this study showed that AMPK/TBC1D1 and IGF1/Akt/AS160 signaling were enhanced by acute RE, and that GLUT4 translocation after acute RE was more prolonged than after acute AE. These results suggest that acute RE-induced increases in intramuscular IGF1 expression might be a distinct regulator of GLUT4 translocation.Wiley-Blackwell Publishing Ltd, 2016年08月, Physiological Reports, 4(16) (16), 英語[査読有り]研究論文（学術雑誌）
- Administration of dehydroepiandrosterone (DHEA), a precursor of sex steroid hormones, reduces total and visceral fat mass and elevates adipocytic adiponectin gene expression. The aim of this study is to investigate whether levels of peroxisome proliferator-activated receptors (PPARs) in muscle and transcription of PPAR target genes are affected by long-term DHEA administration or exercise training, and whether altered PPAR levels are associated with circulating adiponectin level in obese rats. After 14 weeks on a high-sucrose diet, obese male Wistar rats were assigned randomly to one of 3 groups: control, DHEA administration (1 mg/kg body weight), or exercise training (treadmill running for 1h, 25m/min, 5 days/week) for 6 weeks (n=7 for each group). Plasma DHEA and total adiponectin levels in the DHEA-treated and exercise-training groups were significantly higher than those in the obese control group. Additionally, DHEA administration and exercise training significantly increased muscular PPAR and PPAR protein levels, with a concomitant increase in mRNA expression of 3-hydroxyacyl-CoA dehydrogenase and cytochrome c oxidase IV, which are target genes of PPAR and PPAR respectively. Moreover, DHEA administration increased these protein and mRNA levels to the same degree as exercise training. Circulating adiponectin level was positively correlated with plasma DHEA and with muscle levels of PPAR and PPAR. These results suggest that in obese rats, secretion of adiponectin due to chronic DHEA administration and exercise training may contribute to an increase in the transcription of genes encoding lipid metabolic enzymes, mediated via elevated expression of PPAR and PPAR in muscle.GEORG THIEME VERLAG KG, 2016年03月, Horm Metab Res, 48(3) (3), 207 - 212, 英語[査読有り]研究論文（学術雑誌）
- Objectives: Kamishimotsuto (KST) is a supplement containing 13 different herbs including Phellodendron bark, Anemarrhena rhizome and ginseng that have been shown to activate mammalian target of rapamycin complex 1 (mTORC1) and thereby increase muscle protein synthesis in vitro. However, the combined effect of KST and resistance exercise on muscle protein anabolism has not been investigated in vivo. Therefore, the purpose of this study was to investigate the effect of KST supplementation, resistance exercise on (mTORC1) signaling and subsequent muscle protein synthesis. Methods: Male Sprague-Dawley rats were divided into two groups: one group received KST (500 mg/kg/d in water) and the other group received placebo (PLA) for 7 d. After 12 h of fasting, the right gastrocnemius muscle was isometrically exercised via percutaneous electrical stimulation. Muscle samples were analyzed for muscle protein synthesis (MPS) and by western blotting analysis to assess the phosphorylation of p70S6K (Thr389), rpS6 (Ser240/244), and Akt (Ser473 and Thr308). Results: KST supplementation for 7 d significantly increased basal p-Akt (Ser473) levels compared with PLA, phosphorylation of the signaling proteins and MPS at baseline were otherwise unaffected. p-p70S6K and p-rpS6 levels significantly increased 1 h and 3 h after exercise in the PLA group, and these elevations were augmented in the KST group (P < 0.05). Furthermore, MPS at 6 h after resistance exercise was greater in the KST group than in the PLA group (P < 0.05). Conclusions: While resistance exercise alone was able to increase p70S6K and rpS6 phosphorylation, Kamishimotsuto supplementation further augmented resistance exercise-induced muscle protein synthesis through mTORC1 signaling. (C) 2016 Elsevier Inc. All rights reserved.ELSEVIER SCIENCE INC, 2016年01月, NUTRITION, 32(1) (1), 108 - 113, 英語[査読有り]研究論文（学術雑誌）
- Previous studies have shown that acute exercise elevates sex steroid hormone concentrations in rodents and that sprint exercise increases circulating testosterone in healthy young men. However, the effect of different exercise intensities on sex steroid hormone responses at different levels of physical fitness is still unclear. In this study, we compared circulating sex steroid hormone responses at different exercise intensities in athletes and non-athletes. Eight male endurance athletes and 11 non-athletes performed two 15 min sessions of submaximal exercise at 40 and 70% peak oxygen uptake (V-O2peak), respectively, and exercised at 90%. V-O2peak until exhaustion. Venous blood samples were collected during the last minute of each submaximal exercise session and immediately after exhaustion. Acute exercise at 40, 70 and 90%. V-O2peak induced significant increases in serum dehydroepiandrosterone (DHEA) and free testosterone concentrations in non-athletes. On the contrary, only 90%. V-O2peak exercise led to an increase in serum DHEA and free testosterone concentrations in athletes. Serum 5 alpha-dihydrotestosterone concentrations increased with 90%. V-O2peak exercise in both athletes and non-athletes. Additionally, serum estradiol concentrations were significantly increased at moderate and high exercise intensities in both athletes and non-athletes. These results indicate that in endurance athletes, serum sex steroid hormone concentrations, especially serum DHEA and 5 alpha-dihydrotestosterone concentrations, increased only with high-intensity exercise, suggesting that different responses of sex steroid hormone secretion are induced by different exercise intensities in individuals with low and high levels of physical fitness. In athletes, therefore, high-intensity exercise may be required to increase circulating sex steroid hormone concentrations.WILEY-BLACKWELL, 2016年01月, EXPERIMENTAL PHYSIOLOGY, 101(1) (1), 168 - 175, 英語[査読有り]研究論文（学術雑誌）
- 2015年12月, 睡眠と環境, 10-11(1) (1), 5-14日本人女性を対象としたメタボリックシンドロームリスク因子に対する睡眠および加齢の相互関係（パイロット研究）[査読有り]
- Sarcopenia has never been diagnosed based on site-specific muscle loss, and little is known about the relationship between site-specific muscle loss and metabolic syndrome (MetS) risk factors. To this end, this cross-sectional study aimed to investigate the relationship between site-specific muscle size and MetS risk factors. Subjects were 38 obese men and women aged 40-82 years. Total body fat and lean body mass were assessed by whole-body dual-energy X-ray absorptiometry (DXA) scan. Muscle thickness (MTH) was measured using B-mode ultrasound scanning in six body regions. Subjects were classified into general obesity (GO) and sarcopenic obesity (SO) groups using the threshold values of one standard deviation below the sex-specific means of either MTH or skeletal muscle index (SMI) measured by DXA. MetS risk score was acquired by standardizing and summing the following continuously distributed variables: visceral fat area, mean blood pressure, HbA1c, and serum triglyceride / high density lipoprotein cholesterol, to obtain the Z-score. Multiple regression analysis revealed that the MetS risk score was independently associated with abdominal MTH in all subjects, but not with MTH in other muscle regions, including the thigh. Although HbA1c and the number of MetS risk factors in the SO group were significantly higher than those in the GO group, there were no significant differences between GO and SO groups as defined by SMI. Ultrasound-derived abdominal MTH would allow a better assessment of sarcopenia in obese patients and can be used as an alternative to the conventionally-used SMI measured by DXA.PUBLIC LIBRARY SCIENCE, 2015年12月, PLOS ONE, 10(12) (12), e0143858, 英語[査読有り]研究論文（学術雑誌）
- BACKGROUND Obese and overweight patients are at increased risk of arterial stiffness, and visceral, epicardial and hepatic fat accumulation is associated with cardiovascular disease risk. In general, muscular lipids are stored either in interstitial adipose tissue (extramyocellular lipid (EMCL)) or in lipid droplets within muscle cells (intramyocellular lipid (IMCL)). However, the association between IMCL or EMCL content and arterial stiffness remains unclear. This cross-sectional study aimed to clarify this association. METHODS A total of 237 subjects (18-81 years) were enrolled in this study. The IMCL and EMCL contents of the right vastus lateralis muscle were evaluated by proton magnetic resonance spectroscopy. Arterial stiffness was estimated using brachial-ankle pulse wave velocity (baPWV). RESULTS There were significant correlations between baPWV and the contents of both IMCL (R = -0.23, P < 0.001) and EMCL (R = 0.53, P < 0.001) in all subjects. The baPWV negatively correlated with IMCL content (R = -0.45, P < 0.001) in females only. In contrast, significant positive correlations were observed between baPWV and EMCL content in both males (R = 0.59, P < 0.001) and females (R = 0.55, P < 0.001). IMCL and EMCL contents contributed independently to baPWV variation after adjustment for age, sex, body mass index, visceral and subcutaneous abdominal fat, upper and lower limb fat, blood pressure, heart rate, and lipid profiles. CONCLUSION These results suggest that IMCL and EMCL contents may be a risk factor for arterial stiffness, and this association differed with gender and age.OXFORD UNIV PRESS, 2015年12月, AMERICAN JOURNAL OF HYPERTENSION, 28(12) (12), 1473 - 1479, 英語[査読有り]研究論文（学術雑誌）
- Aging-induced arterial stiffening is reduced by aerobic exercise training, and elevated production of nitric oxide (NO) participates in this effect. Adropin is a regulator of endothelial NO synthase and NO release, and circulating adropin level decreases with age. However, the effect of habitual aerobic exercise on circulating adropin levels in healthy middleaged and older adults remains unclear. We sought to determine whether serum adropin level is associated with exercise training-induced changes in arterial stiffness. First, in a cross-sectional study, we investigated the association between serum adropin level and both arterial stiffness and cardiorespiratory fitness in 80 healthy middle-aged and older subjects (65.6 +/- 0.9 yr). Second, in an intervention study, we examined the effects of 8-wk aerobic exercise training on serum adropin level and arterial stiffness in 40 healthy middle-aged and older subjects (67.3 +/- 1.0 yr) divided into two groups: aerobic exercise training and sedentary controls. In the cross-sectional study, serum adropin level was negatively correlated with carotid beta-stiffness (r = -0.437, P < 0.001) and positively correlated with plasma NOx level (r = 0.493, P < 0.001) and cardiorespiratory fitness (r = 0.457, P < 0.001). Serum adropin levels were elevated after the 8-wk aerobic exercise training intervention, and training- induced changes in serum adropin level were correlated with traininginduced changes in carotid beta-stiffness (r = -0.399, P < 0.05) and plasma NOx level (r = 0.623, P < 0.001). Thus the increase in adropin may participate in the exercise-induced reduction of arterial stiffness.AMER PHYSIOLOGICAL SOC, 2015年11月, AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 309(10) (10), H1642 - H1647, 英語[査読有り]研究論文（学術雑誌）
- Vitamin D and vitamin D receptor (VDR) are involved in the maintenance of skeletal muscle mass and function. Although resistance exercise is well known to enhance muscle growth and improve muscle function, the effect of resistance exercise on VDR has been unclear. We investigated intramuscular VDR expression in response to an acute bout of resistance exercise or endurance exercise. Male adult Sprague-Dawley rats were subjected to either resistance exercise (isometrically exercised via percutaneous electrical stimulation for five sets of ten 3s contractions, with a 7s interval between contractions and 3min rest intervals between sets) or endurance exercise (treadmill at 25mmin(-1) for 60min). Rats were killed immediately or 1, 3, 6 or 24h after completion of the resistance or endurance exercise, and gastrocnemius muscles were removed. Non-exercised control animals were killed in a basal state (control group). Intramuscular VDR expression was significantly higher immediately after resistance exercise and elevated for 3h after exercise compared with the control group (P<0.05), and the resistance exercise significantly increased phosphorylated ERK1/2 and Mnk1 expression (P<0.05), which may be associated with VDR expression, immediately after exercise. Additionally, intramuscular expression of cytochrome P450 27B1, an enzyme related to vitaminD metabolism, was significantly higher at 1 and 3h after exercise (P<0.05) compared with the control group. In contrast, endurance exercise had no effect on any of the measured proteins. Our results indicate that resistance exercise may be an efficient way to increase intramuscular VDR and related enzyme expression.WILEY-BLACKWELL, 2015年10月, EXPERIMENTAL PHYSIOLOGY, 100(10) (10), 1168 - 1176, 英語[査読有り]研究論文（学術雑誌）
- Exercise and caloric restriction (CR) have been reported to have anti-ageing, anti-obesity, and health-promoting effects. Both interventions increase the level of dehydroepiandrosterone (DHEA) in muscle and blood, suggesting that DHEA might partially mediate these effects. In addition, it is thought that either 5'-adenosine monophosphate-activated protein kinase (AMPK) or peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1 alpha) mediates the beneficial effects of exercise and CR. However, the effects of DHEA on AMPK activity and PGC-1 alpha expression remain unclear. Therefore, we explored whether DHEA in myotubes acts as an activator of AMPK and increases PGC-1 alpha. DHEA exposure increased glucose uptake but not the phosphorylation levels of Akt and PKC zeta/lambda in C2C12 myotubes. In contrast, the phosphorylation levels of AMPK were elevated by DHEA exposure. Finally, we found that DHEA induced the expression of the genes PGC-1 alpha and GLUT4. Our current results might reveal a previously unrecognized physiological role of DHEA; the activation of AMPK and the induction of PGC-1 alpha by DHEA might mediate its anti-obesity and health-promoting effects in living organisms. (C) 2015 Elsevier Inc. All rights reserved.ACADEMIC PRESS INC ELSEVIER SCIENCE, 2015年07月, Biochem Biophys Res Commun, 463(1-2) (1-2), 42 - 47, 英語[査読有り]研究論文（学術雑誌）
- (一社)日本体力医学会, 2015年04月, 体力科学, 64(2) (2), 285 - 285, 日本語高強度クロス運動トレーニングが糖負荷試験成績に与える影響に関する研究
- Aging-induced elevation in C1q secretion activates the Wnt signaling pathway in muscles, leading to the development of muscle fibrosis. However, the association between serum C1q level and muscle mass and strength remains unclear in humans. The aim of the study was to elucidate whether serum C1q level is associated with aging-and resistance training-induced changes in muscle mass and strength. First, in a cross-sectional study, we investigated the association between serum C1q level and muscle mass and strength in 131 healthy subjects, aged 20-81 yr. Second, in an intervention study, we examined the association between the effects of serum C1q level and muscle mass and strength on 12wk resistance training in 11 healthy older adults (60-81 yr). In the cross-sectional study, serum C1q level increased with aging and was negatively correlated with muscle mass and strength. Furthermore, 12 wk resistance training in older adults reduced the age-associated elevation in serum C1q levels. The training effect of serum C1q level significantly correlated with the change in the cross-sectional area of the thigh (r = -0.703; P < 0.01). Serum C1q level may reflect loss of muscle mass; therefore, C1q may be a novel biomarker of sarcopenia.FEDERATION AMER SOC EXP BIOL, 2015年03月, FASEB JOURNAL, 29(3) (3), 1003 - 1010, 英語[査読有り]研究論文（学術雑誌）
- To elucidate the effects of endurance training on circulating irisin levels in young and middle-aged/older adults, and to determine the association between endurance training-induced alteration of irisin and reduction in body fat. Twenty-five healthy young (age 21 +/- 1 years; 16 men, 9 women) and 28 healthy middle-aged/older adults (age 67 +/- 8 years; 12 men, 16 women) participated in the study. Each age cohort was divided into two groups: the endurance-training group (14 young, 14 middle-aged/older) and the control group. Subjects in the training groups completed an 8-week endurance-training program (cycling at 60-70% peak oxygen uptake [VO2peak] for 45 min, 3 days/week). Before and after the intervention, we evaluated serum irisin level, VO2peak, and body composition. The increase in VO2peak in the young and middle-aged/older training groups after the intervention period was significantly greater than those in the young and middle-aged/older control groups (P < 0.05). Serum irisin level was significantly increased in the middle-aged/older training group after the intervention period (P < 0.01), but not in the young training group. Furthermore, in the middle-aged/older training group, the endurance training-induced reduction in visceral adipose tissue area was negatively correlated with the change in serum irisin level (r = -0.54, P < 0.05). These results suggest a possible role for secreted irisin in the exercise-induced alteration of abdominal visceral fat in middle-aged and older adults.PUBLIC LIBRARY SCIENCE, 2015年03月, PLOS ONE, 10(3) (3), 英語[査読有り]研究論文（学術雑誌）
- (NPO)日本スポーツ栄養学会, 2015年01月, 日本スポーツ栄養研究誌, 8, 70 - 70, 日本語高校生女子陸上競技選手における疲労骨折リスクと骨密度に関する検討
- 2015年, 睡眠と環境(J.Sleep and Environments)Interaction between Sleep Duration and Age on Metabolic Syndrome Risk Factors in Japanese Women
- 2015年, 肥満研究, 21(3) (3), 167 - 176
- Sex steroid hormones are secreted mainly by the ovary and testis and regulate diverse physiological processes in target tissues. Recent studies have shown that sex steroidogenesis-related mRNA and protein expressions, such as for 17 beta-hydroxysteroid dehydrogenase (HSD), 3 beta-HSD, 5 alpha-reductase and aromatase cytochrome P-450 (P450arom) enzymes, are detected in the skeletal muscle, while testosterone, estradiol, and 5 alpha-dihydrotestosterone (DHT) were locally synthesized in skeletal muscle from dehydroepiandrosterone (DHEA). Moreover, in animal and human studies, the sex steroidogenesis enzymes and sex steroid hormone levels in skeletal muscle are upregulated by acute and chronic exercise stimulation. The enhanced muscle sex steroidgenesis is associated with glycemic control via upregulation of muscle glucose transporter-4 (GLUT-4) signaling in obese and diabetic rats and with muscle mass and strength in older men. Thus, an exercise-induced increase of sex steroid hormone in muscle may positively impact age-related concerns such as life-related diseases and sarcopenia. (c) 2014 Elsevier Ltd. All rights reserved.PERGAMON-ELSEVIER SCIENCE LTD, 2015年01月, JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 145, 200 - 205, 英語[査読有り][招待有り]研究論文（学術雑誌）
- Objectives. - Low-circulating testosterone is associated with development of type 2 diabetes in obese men. In this study, we examined the effects of experimental overfeeding and weight gain on serum levels of sex hormones and skeletal muscle expression of steroidogenic enzymes in healthy men with (FH+) and without (FH-) a family history of type 2 diabetes. Methods. - Following a 3-day lead in energy balanced diet, FH+ (n = 9) and FH- men (n = 11) were overfed by 5200 kJ/day (45% fat) for 28 days. Body weight, fasting glucose, insulin, sex steroid, sex hormone binding globulin (SHBG) levels, insulin sensitivity (hyperinsulinaemic-euglycaemic clamp) and body fat (DXA) were assessed in all individuals at baseline and day 28, and sex steroidogenesis-related enzyme expression in vastus lateralis biopsies was examined in a subset (n=11). Results. - Body weight, fat mass and fasting insulin levels were increased by overfeeding (P < 0.01) and insulin was increased significantly more in FH+ men (P < 0.01). Serum sex hormone binding globulin (SHBG) and 5 alpha-dihydrotestosterone (DHT) were reduced with overfeeding (P < 0.05), and serum testosterone and DHT were reduced to a greater extent in FH+ men (P < 0.05). Overfeeding reduced mRNA expression of 3 beta-hydroxysteroid dehydrogenase (HSD) and 17 beta HSD (P <= 0.007), independently of group. 5 alpha-Reductase (SRD5A1) mRNA expression was not changed overall, but a time by group interaction was observed (P = 0.04). Conclusion. - Overfeeding reduced SHBG and muscle expression of enzymes involved in the formation of testosterone in skeletal muscle. Men with a family history of T2DM were more susceptible to deleterious outcomes of overfeeding with greater reductions in serum testosterone and DHT and greater increases in markers of insulin resistance, which may contribute to increased risk of developing type 2 diabetes. (C) 2014 Elsevier Masson SAS. All rights reserved.MASSON EDITEUR, 2014年12月, DIABETES & METABOLISM, 40(6) (6), 439 - 444, 英語[査読有り]研究論文（学術雑誌）
- Acute dehydroepiandrosterone (DHEA) administration improves hyperglycemia in rats with streptozotocin (STZ)-induced type 1 diabetes mellitus. Diosgenin, a steroid structurally similar to DHEA (dehydroepiandrosterone), is contained highly levels in dioscorea; however, it is still unclear whether this natural product improves hyperglycemia in the type 1 diabetes model rats through an increase muscular GLUT4 signaling. After 1 week of STZ injection, fasting glucose level was measured in blood taken from the tail vein every 30 min for 150 min after injection of diosgenin or dioscorea (3 mg/kg). On another day, muscle was resected 150 min after diosgenin or dioscorea injections. Serum DHEA level increased significantly 120 min after diosgenin or dioscorea injections; concomitantly, blood glucose level decreased significantly. Moreover, GLUT4 translocation, as well as phosphorylation of Akt and PKC zeta/lambda, increased significantly by diosgenin or dioscorea administration. However, these effects of diosgenin and dioscorea were blocked by a 5 alpha-reductase inhibitor that inhibits synthesizing dehydrotestosterone (DHT) from testosterone. Additionally, significant correlations were observed between blood glucose level, GLUT4 translocation level, and muscular sex steroid hormone level 150 min after the administrations. These results suggest that the diosgenin-induced increase in the DHEA level may contribute to the improvement of hyperglycemia by activating the muscular GLUT4 signaling pathway in type 1 diabetes model rats. (C) 2014 Elsevier Ltd. All rights reserved.PERGAMON-ELSEVIER SCIENCE LTD, 2014年09月, JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 143, 152 - 159, 英語[査読有り]研究論文（学術雑誌）
- Concurrent training, a combination of endurance (EE) and resistance exercise (RE) performed in succession, may compromise the muscle hypertrophic adaptations induced by RE alone. However, little is known about the molecular signaling interactions underlying the changes in skeletal muscle adaptation during concurrent training. Here, we used an animal model to investigate whether EE before or after RE affects the molecular signaling associated with muscle protein synthesis, specifically the interaction between RE-induced mammalian target of rapamycin complex 1 (mTORC1) signaling and EE-induced AMP-activated protein kinase (AMPK) signaling. Male Sprague-Dawley rats were divided into five groups: an EE group (treadmill, 25 m/min, 60 min), an RE group (maximum isometric contraction via percutaneous electrical stimulation for 3 X 10 s, 5 sets), an EE before RE group, an EE after RE group, and a nonexercise control group. Phosphorylation of p70S6K, a marker of mTORC1 activity, was significantly increased 3 h after RE in both the EE before RE and EE after RE groups, but the increase was smaller in latter. Furthermore, protein synthesis was greatly increased 6 h after RE in the EE before RE group. Increases in the phosphorylation of AMPK and Raptor were observed only in the EE after RE group. Akt and mTOR phosphorylation were increased in both groups, with no between-group differences. Our results suggest that the last bout of exercise dictates the molecular responses and that mTORC1 signaling induced by any prior bout of RE may be downregulated by a subsequent bout of EE.AMER PHYSIOLOGICAL SOC, 2014年05月, AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 306(10) (10), E1155 - E1162, 英語研究論文（学術雑誌）
- Skeletal muscle can synthesize testosterone and 5α-dihydrotestosterone (DHT) from dehydroepiandrosterone (DHEA) via steroidogenic enzymes in vitro, but hormone levels and steroidogenic enzyme expression decline with aging. Resistance exercise has been shown to increase in plasma sex steroid hormone levels. However, it remains unclear whether resistance training can restore impaired steroidogenic enzyme expressions in older individuals. Six young and 13 older men were recruited, and muscle biopsies were taken from the vastus lateralis at basal state. The same group of older subjects underwent resistance training involving knee extension and flexion exercises for 12 wk, and post-training biopsies were performed 4-5 d after the last exercise session. Muscular sex steroid hormone levels and sex steroidgenesis-related enzyme expressions were significantly lower in older subjects than younger ones at baseline, but 12 wk of resistance training significantly restored hormone levels (DHEA: 432±26 at baseline, 682±31 pg/μg protein, DHT: 6.2±0.9 at baseline, 9.8±1.4 pg/μg protein). Furthermore, the steroidogenesis-related enzymes such as 3β-hydroxysteroid dehydrogenase (HSD), 17β-HSD, and 5α-reductase expressions were significantly restored by resistance training. We conclude progressive resistance training restores age-related declines in sex steroidogenic enzyme and muscle sex steroid hormone levels in older men.2014年04月, FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 28(4) (4), 1891 - 7, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Aging-induced deterioration of arterial stiffness is decreased by regular exercise, and increased nitric oxide (NO) production participates in this effect. Apelin regulates endothelial NO synthase in endothelial cells, promoting NO production. However, the effect of aerobic exercise training on circulating apelin levels in healthy middle-aged and older adults remains unknown. Accordingly, this study aimed to clarify the effects of regular aerobic exercise on apelin concentrations in middle-aged and older adults. Thirty-four healthy middle-aged and older subjects (67.0 +/- 1.3 years) were randomly divided into two groups: exercise intervention and sedentary controls. Subjects in the training group completed 8-week of aerobic exercise training (60-70% peak oxygen uptake [VO2peak] for 45 min, 3 days/week). Before and after the intervention, we evaluated plasma apelin and nitrite/nitrate (NOx) concentrations, VO2peak, and arterial stiffness index. In the training group, VO2peak was significantly increased, and carotid beta-stiffness was significantly decreased, after the intervention (P<0.05). Moreover, plasma apelin and NOx levels were significantly increased in the training group after the intervention (P<0.05). Additionally, there was a correlation between the training effects of plasma apelin levels and carotid beta-stiffness (r = -0.508, P = 0.032) and plasma NOx levels (r = 0.494, P = 0.037). By contrast, none of these parameters changed significantly in the control group. These results suggest that the increased in plasma apelin levels may be associated with exercise training-induced alternation of arterial stiffness in middle-aged and older adults.PUBLIC LIBRARY SCIENCE, 2014年04月, PLOS ONE, 9(4) (4), 英語[査読有り]研究論文（学術雑誌）
- The Japanese Society of Physical Fitness and Sports Medicine, 2014年, 体力科学, 63(1) (1), 72 - 72, 日本語
- The Japanese Society of Physical Fitness and Sports Medicine, 2014年, 体力科学, 63(1) (1), 91 - 91, 日本語
- Recent studies indicate that matrix metalloproteinases (MMPs) and critical linkage proteins in the extracellular matrix (ECM) regulate skeletal muscle mass, although the effects of resistance training (RT) on protein expression and activity are unclear. Thus, the purpose of the present study was to investigate the effects of RT on MMP activity and expression of ECM-related proteins. Ten male Sprague-Dawley rats were randomly assigned to 1 bout (1B) or 18 bouts (18B) of electrical stimulation. The right gastrocnemius muscle was isometrically contracted via percutaneous electrical stimulation (five sets of 5 sec stimulation × five contractions/set with 5 sec interval between contractions and 3 min rest between sets) once (1B) or every other day for 5 weeks (18B). The left leg served as a control. Activity of MMP-2 and MMP-9, determined via gelatin zymography, was increased (P < 0.05) immediately after 1B. However, MMP activation was not evident following 18B. No changes in collagen IV, laminin α2, α7-integrin, or ILK protein expression were detected immediately following 1B or 18B. However, β1-integrin protein expression was significantly increased (P < 0.05) with 18B. Our results suggest that resistance exercise activates MMPs during the initial phase of RT but this response is attenuated with continuation of RT.American Physiological Society, 2014年, Physiological reports, 2(11) (11), 英語[査読有り]研究論文（学術雑誌）
- 2014年, FASEB JOURNAL, 28(4) (4), 1891 - 1897, 英語[査読有り]研究論文（学術雑誌）
- A recent study identified ursolic acid (UA) as a potent stimulator of muscle protein anabolism via PI3K/Akt signaling, thereby suggesting that UA can increase Akt-independent mTOR complex 1 (mTORC1) activation induced by resistance exercise via Akt signaling. The purpose of the present study was to investigate the effect of UA on resistance exercise-induced mTORC1 activation. The right gastrocnemius muscle of male Sprague-Dawley rats aged 11 wk was isometrically exercised via percutaneous electrical stimulation (stimulating ten 3-s contractions per set for 5 sets), while the left gastrocnemius muscle served as the control. UA or placebo (PLA; corn oil only) was injected intraperitoneally immediately after exercise. The rats were killed 1 or 6 h after the completion of exercise and the target tissues removed immediately. With placebo injection, the phosphorylation of p70(S6K) at Thr(389) increased 1 h after resistance exercise but attenuated to the control levels 6 h after the exercise. On the other hand, the augmented phosphorylation of p70(S6K) was maintained even 6 h after exercise when UA was injected immediately after exercise. A similar trend of prolonged phosphorylation was observed in PRAS40 Thr(246), whereas UA alone or resistance exercise alone did not alter its phosphorylation level at 6 h after intervention. These results indicate that UA is able to sustain resistance exercise-induced mTORC1 activity.AMER PHYSIOLOGICAL SOC, 2013年09月, AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 305(6) (6), E760 - E765, 英語[査読有り]研究論文（学術雑誌）
- We examined the effects of exercise training on the levels of lipid droplet (LD)-associated and mitochondria-related proteins in diet-induced obese (DIO) rats. Furthermore, we assessed putative factors induced by exercise to activate lipolysis in differentiated 3T3-L1 adipocytes. DIO Wistar male rats (age 20 wk) were divided into sedentary control (SED, n = 7) and exercise training (EX, n = 7) groups. EX animals were subjected to treadmill running (25 m/min, 1 h/day, 5 days/wk) for 6 wk. Epididymal fat was dissected and used for protein analyses. 3T3-L1 adipocytes were incubated with media containing hydrogen peroxide (H2O2), sodium-lactate, caffeine, AICAR, or SNAP (NO donor) for 6 h, or 1 mM H2O2 for 15 min, followed by incubation with normal media for up to 24 h total. Protein expression levels and lipolytic activities were biochemically assayed. Epididymal fat significantly decreased in EX animals compared with SED animals. Levels of cytochrome c oxidase (COx), perilipin, hormone sensitive lipase (HSL), and adipose triglyceride lipase (ATGL) proteins in epididymal fat pads of EX animals were significantly increased compared with those in SED animals. In 3T3-L1 cells, glycerol or fatty acid release was significantly increased by all treatments. Lactate or SNAP significantly increased PGC-1 alpha expression, and H2O2 significantly increased COx protein levels compared with controls. Expression of perilipin, HSL, ATGL, or comparative gene identification (CGI)-58 was significantly increased by all treatments. By increasing lipolytic activity in adipocytes, the exercise-inducible factors are attractive therapeutic effectors against LD-associated metabolic diseases.AMER PHYSIOLOGICAL SOC, 2013年07月, JOURNAL OF APPLIED PHYSIOLOGY, 115(2) (2), 260 - 267, 英語[査読有り]研究論文（学術雑誌）
- 社団法人日本体育学会, 2013年, 日本体育学会大会予稿集, 64, 172 - 172, 日本語
- 社団法人日本体育学会, 2013年, 日本体育学会大会予稿集, 64, 165 - 165, 日本語
- 社団法人日本体育学会, 2013年, 日本体育学会大会予稿集, 64, 171 - 171, 日本語
- 社団法人日本体育学会, 2013年, 日本体育学会大会予稿集, 64, 174 - 174, 日本語
- 社団法人日本体育学会, 2013年, 日本体育学会大会予稿集, 64, 416 - 416, 日本語
- 社団法人日本体育学会, 2013年, 日本体育学会大会予稿集, 64, 184 - 185, 日本語
- 2013年, JATI EXPRESS, 34性ホルモンに着目した運動と食事療法
- 杏林書院, 2013年, 体育の科学, 63(8) (8), 616-622 - 622, 日本語骨格筋由来の性ステロイドホルモンの新たな役割
- The ability of exercise to improve hyperglycemia by enhancing glucose metabolism in the skeletal muscle of type 2 diabetic patients is well established. We reported sex steroid hormones can be locally synthesized in skeletal muscle and decrease fasting blood glucose levels in obese rats. Here, we determined whether exercise-induced production of sex steroid hormones in skeletal muscle could directly reverse hyperglycemia in the Zucker diabetic fatty rat model using osmotic mini pump. Thirty Zucker diabetic fatty rats were randomly assigned to the following groups: control, exercise, or exercise with continuous infusion of 5α-reductase inhibitor. The results indicated 6 weeks of exercise significantly reduced serum insulin and fasting glucose levels compared to control group. Dehydroepiandrosterone, 5α-dehydrotestosterone, and 5α-reductase levels were all significantly higher in skeletal muscle of the exercise group. Moreover, exercise increased glucose transporter-4 translocation with a concomitant upregulation of phosphorylated phosphoinositide 3-kinase, protein kinase B and C-ζ/λ. Furthermore, significant correlations were observed between fasting glucose and muscular DHT levels. Interestingly, the observed exercise-induced improvements in serum insulin and fasting glucose levels were all suppressed by administration of 5α-reductase inhibitor. These results indicated the exercise-induced improvements in glucose metabolism signaling and glucose levels may be directly attributed to the increased levels of sex steroid hormones within skeletal muscles.OMICS International, 2013年, Journal of Diabetes and Metabolism, 4(1) (1), 1 - 239, 英語[査読有り]研究論文（学術雑誌）
- Sarcopenia is the age-associated loss of skeletal muscle mass and strength that develops slowly over decades and becomes a significant factor to disability among the elderly population. Recent studies have indicated that blunted anabolic response to nutritional stimuli significantly contributes to the development of sarcopenia. In the present article, we will review recent findings on the role of nutritional intake on muscle protein metabolism in the elderly. This review will particularly focus on acute anabolic responses to amino acids and protein intake, age-associated changes in the response of muscle protein to meal intake, and the role of insulin resistance in muscle protein metabolism. The relationship between age-associated decline of sex steroid hormones and muscle anabolism will also be discussed in addition to the benefits of resistance exercise in muscle protein anabolism. Additionally, recent evidence on the time-course of anabolic response, molecular regulation of muscle protein synthesis, and long-term training effects will be discussed. Finally, recent evidence on the cumulative effect of resistance exercise in combination with nutritional supplementation on muscle protein metabolism will be discussed to propose possible preventative measures against sarcopenia.The Japanese Society of Physical Fitness and Sports Medicine, 2013年, TheJournal of Physical Fitness and Sports Medicine., 2(3) (3), 295 - 300, 英語
- Objective: alpha-Glycerophosphocholine (GPC) is a putative acetylcholine precursor that potentially increases growth hormone secretion through the action of acetylcholine-stimulated catecholamine. The aim of this study was to investigate acute physiologic responses to a single intake of GPC. Methods: Eight healthy male subjects (25 +/- 1 y old) ingested GPC 1000 mg or a placebo in a double-blind randomized crossover study. Fasting blood samples were obtained before the administration of GPC (baseline) and 60 and 120 min after administration. All subjects repeated the identical protocol using the placebo. Results: Plasma free choline levels significantly increased at 60 and 120 min after GPC administration. Plasma growth hormone secretion was increased significantly 60 min after taking GPC, whereas no significant change was observed with the placebo. In addition, the serum free fatty acid was increased 120 min after GPC ingestion, but no changes were seen with the placebo. Moreover, serum acetoacetate and 3-hydroxybutyrate levels, which are indices of hepatic fat oxidation, were increased at 120 min after taking GPC, whereas the placebo had no effect. Conclusion: These findings suggest that a single dose of GPC increases growth hormone secretion and hepatic fat oxidation, with concomitant increases in choline levels, in young adults. (C) 2012 Elsevier Inc. All rights reserved.ELSEVIER SCIENCE INC, 2012年11月, NUTRITION, 28(11-12) (11-12), 1122 - 1126, 英語[査読有り]研究論文（学術雑誌）
- Background: Dehydroepiandrosterone (DHEA) is precursor of sex steroid hormone. We demonstrated that acute DHEA injection to type 1 diabetes model rats induced improvement of hyperglycemia. However, the effect of the combination of DHEA administration and exercise training on insulin resistance is still unclear. This study was undertaken to determine whether 6-weeks of DHEA administration and/or exercise training improve insulin resistance in obese male rats. Methods: After 14 weeks of a high-sucrose diet, obese male Wistar rats were assigned randomly to one of four groups: control, DHEA administration, exercise training, and a combination of DHEA administration and exercise training (n = 10 each group). Results: After 6-weeks of DHEA administration and/or exercise training, rats in the combination group weighed significantly less and had lower serum insulin levels than rats in the other groups. Moreover, the rats treated with DHEA alone or DHEA and exercise had significantly lower fasting glucose levels (combination, 84 +/- 6.5 mg/dL; DHEA, 102 +/- 9.5 mg/dL; control, 148 +/- 10.5 mg/dL). In addition, insulin sensitivity check index showed significant improvements in the combination group (combination, 0.347 +/- 0.11; exercise, 0.337 +/- 0.16%; DHEA, 0.331 +/- 0.14; control, 0.308 +/- 0.12). Muscular DHEA and 5 alpha-dihydrotestosterone (DHT) concentrations were significantly higher in the combination group, and closely correlated with the quantitative insulin-sensitivity check index (DHEA: r = 0.71, p < 0.01; DHT: r = 0.69, p < 0.01). Conclusion: These results showed that a combination of DHEA administration and exercise training effectively improved fasting blood glucose and insulin levels, and insulin sensitivity, which may reflect increased muscular DHEA and DHT concentrations.BIOMED CENTRAL LTD, 2012年05月, NUTRITION & METABOLISM, 9, 英語[査読有り]研究論文（学術雑誌）
- 社団法人日本体育学会, 2012年, 日本体育学会大会予稿集, 63, 145 - 145, 日本語
- 2012年, JATI EXPRESS, 29２型糖尿病患者におけるレジスタンス運動の効果.
- Sato K, Iemitsu M, Aizawa K, Mesaki N, Fujita S. Increased muscular dehydroepiandrosterone levels are associated with improved hyperglycemia in obese rats. Am J Physiol Endocrinol Metab 301: E274-E280, 2011. First published February 1, 2011; doi:10.1152/ajpendo.00564.2010.-This study was undertaken to assess the effects of dehydroepiandrosterone (DHEA) administration and exercise training on muscular DHEA and 5 alpha-dihydrotestosterone (DHT) levels and hyperglycemia in diet-induced obese and hyperglycemic rats. After 14 wk of a high-sucrose diet, obese male Wistar rats were assigned randomly to one of three 6-wk regimens: control, DHEA treatment, or exercise training (running at 25 m/min for 1 h, 5 days/wk; n = 10 each group). Results indicate that either 6 wk of DHEA treatment or exercise training significantly attenuated serum insulin and fasting glucose levels compared with the control group. Plasma and muscle concentrations of DHEA and DHT and expression levels of 5 alpha-reductase were significantly higher in the DHEA-treated and exercise-training groups. Moreover, both DHEA administration and exercise training upregulated GLUT4 translocation with concomitant increases in protein kinase B and protein kinase C zeta/lambda phosphorylation. Muscle DHEA and DHT concentrations closely correlated with blood glucose levels (DHEA treatment: r = -0.68, P < 0.001; exercise training: r = -0.65, P < 0.001), serum insulin levels, and activation of the GLUT4-regulated signaling pathway. Thus, increased levels of muscle sex steroids may contribute to improved fasting glucose levels via upregulation of GLUT4-regulated signaling in diet-induced obesity and hyperglycemia.AMER PHYSIOLOGICAL SOC, 2011年08月, AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 301(2) (2), E274 - E280, 英語[査読有り]研究論文（学術雑誌）
- It is not known whether local androgen metabolism is involved in the mechanisms underlying the dehydroepiandrosterone (DHEA) administration-induced improvement of bone mineral density (BMD) in an estrogen-deficiency state. The aim of the present study was to clarify whether DHEA administration would improve local androgen metabolism and BMD in cancellous site of tibia of ovariectomized (OVX) rats. Twenty-two female rats, 6 weeks old, were randomized into three groups: sham-operated rats, OVX control rats, and OVX rats that received DHEA treatment. DHEA was administered intraperitoneally at 20 mg/kg body weight for 8 weeks. The concentrations of free testosterone and dihydrotestosterone (DHT) in cancellous site of tibia did not change as a result of ovariectomy, while the DHT concentration increased following DHEA administration. We revealed that DHEA administration improved the reduction of 17 beta- and 3 beta-hydroxysteroid dehydrogenases and clearly reversed the reduction of 5 alpha-reductase types 1 and 2 and androgen receptor in the cancellous site of tibia of OVX rats. DHEA administration suppressed estrogen deficiency relative to the decrease in the cancellous BMD, which was positively associated with local DHT concentration. These findings indicate that DHEA administration enhances local bioactive androgen metabolism in the cancellous tibia of young OVX rats, suggesting that local DHT may play a part in the DHEA administration-induced improvement of cancellous BMD.SPRINGER, 2011年08月, CALCIFIED TISSUE INTERNATIONAL, 89(2) (2), 105 - 110, 英語[査読有り]研究論文（学術雑誌）
- Objective: Aerobic exercise on land decreases arterial stiffness? however? the effect of exercise in water on arterial stiffness has not been clear. This study investigated the effect of a 15- min cycling exercise on land and that in water on pulse wave velocity (PWV) as an index of arterial stiffness. Methods: Nine healthy young men were randomly performed exercise on land and exercise in water equivalent to 50% of each maximum oxygen uptake on separate days. The PWV from carotid to femoral artery (aortic PWV) and femoral to posterior tibial artery (leg PWV) were measured at baseline and 15? 30? 60 min after exercise. Results: The heart rate in water was significantly lower during exercise than that on land. In addition? the carbon dioxide output and respiratory exchange ratio during exercise were significantly greater in water. Body temperature increased after the exercise on land but decreased after the exercise in water. Although the aortic PWV and leg PWV decreased concomitantly with decrease in SBP and DBP after the exercise on land? there were no significant changes after the exercise in water. Conclusion: Although acute exercise on land decreased arterial stiffness after exercise? acute exercise in water of the same exercise load did not. The differences in body temperature and blood pressure after exercise may result in diverse arterial stiffness after exercise.Japanese Society of Physical Fitness and Sports Medicine, 2011年, 体力科学, 60(3) (3), 269 - 277, 日本語[査読有り]研究論文（学術雑誌）
- Androgens, Such as testosterone, play important roles in regulation of diverse physiological process of target tissues. Recently, we reported that steroidogenic enzymes exist in skeletal Muscle and regulate local production of testosterone in response to exercise. Testosterone is transformed into a bioactive androgen metabolite, dihydrotestosterone (DHT) by 5 alpha-reductase. However, it is unclear whether exercise stimulates local bioactive androgen metabolism in the skeletal muscle in both sexes. In the present study, we examined sex differences in the levels of dehydroepiandrosterone (DHEA), free testosterone, DHT, and steroidogenesis-related enzymes 5 alpha-reductase and androgen receptor (AR) in rat's skeletal muscle before and after a single bout of exercise. Basal muscular free testosterone and DHT levels were higher in males than females, whereas the levels of DHEA did not differ between the sexes. Muscular DHEA, free testosterone, and DHT levels were increased in both sexes after the exercise. There were no differences of 5 alpha-reductase and AR transcripts and proteins between the sexes, and the expression of 5 alpha-reductase was significantly increased in both sexes after the exercise. Finally, the expression of AR was significantly higher in female rats, but not in males after the exercise. These data Suggest that acute exercise enhances the local bioactive androgen metabolism in the skeletal Muscle of both sexes. (C) 2009 Elsevier Inc. All rights reserved.ELSEVIER SCIENCE INC, 2010年03月, STEROIDS, 75(3) (3), 219 - 223, 英語[査読有り]研究論文（学術雑誌）
- (一社)日本体力医学会, 2009年12月, 体力科学, 58(6) (6), 780 - 780, 日本語中高齢男性における性ホルモンと動脈スティフネスの関係に運動習慣が及ぼす影響
- Aim: Addition of dehydroepiandrosterone (DHEA) to a cultured skeletal muscle locally synthesizes 5 alpha-dihydrotestosterone (DHT). It induced activation of glucose metabolism-related signalling pathway via protein kinase B (Akt) and protein kinase C zeta/lambda (PKC zeta/lambda)-glucose transporter-4 (GLUT4) proteins. However, such an effect of DHEA in vivo remains unclear. Methods: Using streptozotocin (STZ)-induced rats with type 1 diabetes mellitus, we tested the hypothesis that a single bout of DHEA injection in the rats improves hyperglycaemia and muscle GLUT4-regulated signalling pathway. After 1 week of STZ injection (55 mg kg-1) with male Wistar rats, fasting glucose concentrations were determined in a blood sample taken from the tail vein. Blood glucose levels were then monitored for 180 min after DHEA or sesame oil (control) was injected (n = 10 for each group). Results: Blood glucose levels decreased significantly for 30-150 min after 2 mg DHEA injection in the STZ rats. In the skeletal muscle, expression and translocation of GLUT4 protein, phosphorylation of Akt and PKC zeta/lambda, and phosphofructokinase and hexokinase enzyme activities increased significantly by DHEA injection. However, DHEA-induced improvements in Akt and PKC zeta/lambda-GLUT4 pathways were blocked by a DHT inhibitor. Conclusion: These results suggest that a single bout of DHEA injection can improve hyperglycaemia and activate the glucose metabolism-related signalling pathway via Akt and PKC zeta/lambda-GLUT4 proteins of skeletal muscles in rats. Moreover, these results show that a DHEA-induced increase in muscle glucose uptake and utilization might contribute to improvement in hyperglycaemia in type 1 diabetes mellitus.WILEY-BLACKWELL PUBLISHING, INC, 2009年11月, Acta Physiologica (oxf), 197(3) (3), 217 - 225, 英語[査読有り]研究論文（学術雑誌）
- Circulating dehydroepiandrosterone (DHEA) is converted to testosterone or estrogen in the target tissues. Recently, we demonstrated that skeletal muscles are capable of locally synthesizing circulating DHEA to testosterone and estrogen. Furthermore, testosterone is converted to 5 alpha-dihydrotestosterone (DHT) by 5 alpha-reductase and exerts biophysiological actions through binding to androgen receptors. However, it remains unclear whether skeletal muscle can synthesize DHT from testosterone and/or DHEA and whether these hormones affect glucose metabolism-related signaling pathway in skeletal muscles. We hypothesized that locally synthesized DHT from testosterone and/or DHEA activates glucose transporter-4 (GLUT-4)-regulating pathway in skeletal muscles. The aim of the present study was to clarify whether DHT is synthesized from testosterone and/or DHEA in cultured skeletal muscle cells and whether these hormones affect the GLUT-4-related signaling pathway in skeletal muscles. In the present study, the expression of 5 alpha-reductase mRNA was detected in rat cultured skeletal muscle cells, and the addition of testosterone or DHEA increased intramuscular DHT concentrations. Addition of testosterone or DHEA increased GLUT-4 protein expression and its translocation. Furthermore, Akt and protein kinase C-zeta/lambda ( PKC-zeta/lambda) phosphorylations, which are critical in GLUT-4-regulated signaling pathways, were enhanced by testosterone or DHEA addition. Testos-terone and DHEA-induced increases in both GLUT-4 expression and Akt and PKC-zeta/lambda phosphorylations were blocked by a DHT inhibitor. Finally, the activities of phosphofructokinase and hexokinase, main glycolytic enzymes, were enhanced by testosterone or DHEA addition. These findings suggest that skeletal muscle is capable of synthesizing DHT from testosterone, and that DHT activates the glucose metabolism-related signaling pathway in skeletal muscle cells.AMER PHYSIOLOGICAL SOC, 2008年05月, AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 294(5) (5), E961 - E968, 英語[査読有り]研究論文（学術雑誌）
- (一社)日本骨代謝学会, 2017年07月, 日本骨代謝学会学術集会プログラム抄録集, 35回, 160 - 160, 日本語2型糖尿病モデルラットに対する長期的なレジスタンス運動が骨組織に与える効果
- AMER DIABETES ASSOC, 2017年06月, DIABETES, 66, A191 - A191, 英語Chronic Resistance Training-Induced Increase in Insulin-Stimulated Akt Activation and GLUT4 Translocation Is Associated with APPL1 Expression in Skeletal Muscle of Type 2 Diabetic Rat研究発表ペーパー・要旨（国際会議）
- 石本記念デサントスポーツ科学振興財団事務局, 2017年05月, デサントスポーツ科学, 38, 28 - 34, 日本語1型糖尿病における運動誘発性遅発低血糖の発症機序の解明 : 1型糖尿病患者の新規運動ガイドライン作成に向けて
- 石本記念デサントスポーツ科学振興財団事務局, 2017年05月, デサントスポーツ科学, 38, 245 - 251, 日本語高齢者における性ステロイドホルモン分泌増加法の開発 : 武道に着目した検討
- LIPPINCOTT WILLIAMS & WILKINS, 2016年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 48(5) (5), 582 - 582, 英語研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2016年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 48(5) (5), 192 - 192, 英語研究発表ペーパー・要旨（国際会議）
- (一社)日本糖尿病学会, 2016年04月, 糖尿病, 59(Suppl.1) (Suppl.1), S - 482, 日本語単回レジスタンス運動に伴うAMPKのリン酸化及びGLUT4のトランスロケーションは一型糖尿病モデルラットの骨格筋で減弱しない
- (一社)日本体力医学会, 2015年12月, 体力科学, 64(6) (6), 602 - 602, 日本語レジスタンス運動はGLUT4のトランスロケーションをより長時間促進する 有酸素性運動との比較
- (公社)日本生化学会, 2015年12月, 日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, 88回・38回, [2LBA097] - [2LBA097], 日本語In vivo骨格筋最大収縮に伴う細胞内糖取り込み制御因子の応答
- (公社)日本生化学会, 2015年12月, 日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, 88回・38回, [2LBA083] - [2LBA083], 英語3T3-L1細胞において、dehydroepiandrosteroneはAMPキナーゼの活性化を介して脂肪細胞分化を抑制する
- (一社)日本体力医学会, 2015年12月01日, 体力科学, 64(6) (6), 583 - 583, 日本語局所的なストレッチ運動が動脈スティフネスに及ぼす影響
- LIPPINCOTT WILLIAMS & WILKINS, 2015年11月, CIRCULATION, 132, 英語Serum Adropin Level is Associated With Exercise Training-induced Improvement of Arterial Stiffness in Obese Adults研究発表ペーパー・要旨（国際会議）
- 石本記念デサントスポーツ科学振興財団事務局, 2015年06月, デサントスポーツ科学, 36, 106 - 114, 日本語最大無酸素性作業能力の向上に関連する分子メカニズムの解明 : 骨格筋発現タンパクの網羅的解析
- AMER DIABETES ASSOC, 2015年06月, DIABETES, 64, A200 - A200, 英語Delayed Onset of Hypoglycemia after an Acute Bout of Exercise Is Associated with Prolonged Muscle GLUT-4 Translocation in Type 1 Diabetes研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2015年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 47(5) (5), 94 - 94, 英語研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2015年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 47(5) (5), 93 - 93, 英語研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2015年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 47(5) (5), 742 - 742, 英語研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2015年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 47(5) (5), 408 - 408, 英語研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2015年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 47(5) (5), 487 - 487, 英語研究発表ペーパー・要旨（国際会議）
- 2015年, 京都滋賀体育学研究, 31, 11 - 16中高齢男女を対象とした生活活動とメタボリックシンドロームリスクとの関係
- LIPPINCOTT WILLIAMS & WILKINS, 2014年11月, CIRCULATION, 130, 英語Musclular Lipid is Associated With a Risk Factor of Arterial Stiffness研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2014年11月, CIRCULATION, 130, 英語Plasma Apelin Level Contributes Exercise Training-induced Improvement of Arterial Stiffness in the Middle and Older Adults研究発表ペーパー・要旨（国際会議）
- 石本記念デサントスポーツ科学振興財団事務局, 2014年06月, デサントスポーツ科学, 35, 113 - 122, 日本語運動により骨格筋から分泌されるIrisinが内臓脂肪減少に関与するのか?
- LIPPINCOTT WILLIAMS & WILKINS, 2014年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 46(5) (5), 629 - 629, 英語研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2014年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 46(5) (5), 458 - 458, 英語Effects Of Cardiorespiratory Fitness Level, Age, And Sex On Intramyocellular And Extramyocellular Lipid Contents研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2014年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 46(5) (5), 635 - 635, 英語DHEA Administration-induced Increase In Adiponectin Secretion Is Associated With Muscular PPAR-alpha Expression In Obese Rats研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2014年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 46(5) (5), 34 - 34, 英語Exercise Order of Concurrent Endurance and Resistance Exercise Modifies mTOR Signaling in Rat Skeletal Muscle研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2014年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 46(5) (5), 151 - 151, 英語Relationships Among Visceral Adipose Tissue Area Observed In The Multiple-slice, Metabolic Syndrome Risks And Cardiorespiratory Fitness研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2014年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 46(5) (5), 481 - 481, 英語Dioscorea-induced Increase Of Sex Steroid Hormone Improve Hyperglycemia In Type 2 Diabetic Rats研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2014年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 46(5) (5), 731 - 731, 英語Antioxidant Inhibits Resistance Exercise Induced Phosphorylation Of P70s6k In Rats研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2014年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 46(5) (5), 325 - 325, 英語Reduction of Arterial Stiffness by Exercise Training Is Associated with Increasing Plasma Apelin Level研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2014年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 46(5) (5), 587 - 587, 英語研究発表ペーパー・要旨（国際会議）
- (公社)日本栄養・食糧学会, 2014年04月, 日本栄養・食糧学会大会講演要旨集, 68回, 223 - 223, 日本語一過性の有酸素性運動がラット骨格筋内のATF4およびアミノ酸トランスポーターに及ぼす影響
- (一社)日本体力医学会, 2014年02月, 体力科学, 63(1) (1), 146 - 146, 日本語
- (一社)日本体力医学会, 2013年12月, 体力科学, 62(6) (6), 628 - 628, 日本語月経周期が女性アスリートの持久性運動時の血中代謝物質に与える影響
- (一社)日本体力医学会, 2013年12月, 体力科学, 62(6) (6), 505 - 505, 日本語レジスタンス運動後のロイシン高配合必須アミノ酸混合物の摂取はAkt/PRAS40シグナル経路を介してmTORC1を活性化させる
- FEDERATION AMER SOC EXP BIOL, 2013年04月, FASEB JOURNAL, 27, 英語Continuous resistance training alters exercise-induced MMPs activation and expression of ECM components研究発表ペーパー・要旨（国際会議）
- (公社)日本栄養・食糧学会, 2013年04月, 日本栄養・食糧学会大会講演要旨集, 67回, 162 - 162, 日本語ウルソール酸はレジスタンス運動後のラット骨格筋Aktリン酸化を亢進させる
- SPRINGER, 2012年07月, JOURNAL OF GENERAL INTERNAL MEDICINE, 27, 412 - 412, 英語Regulation of Hypertrophic Signaling Pathways to a Low-volume Resistance Exercise in Older Individuals研究発表ペーパー・要旨（国際会議）
- SPRINGER, 2012年07月, JOURNAL OF GENERAL INTERNAL MEDICINE, 27, 299 - 299, 英語Differential Effect of Aging on Acute Hormonal Response to Low-Volume Resistance Exercise研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2012年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 44, 902 - 902, 英語Low-Intensity Resistance Training Using an Elastic Band Improves Muscle Mass and Function in Older People研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2012年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 44, 420 - 420, 英語Exercise Training-induced Improvement Of Impaired Muscle Glucose Metabolism Is Associated With Muscular Sex Steroid Hormone Level In Zucker Fatty Diabetes Rats研究発表ペーパー・要旨（国際会議）
- 2011年12月01日, 体力科学, 60(6) (6), 625, 日本語一過性レジスタンス運動時の筋タンパク質合成関連シグナル経路調節‐加齢の影響
- ライフサイエンス出版(株), 2011年11月, Osteoporosis Japan, 19(Suppl.1) (Suppl.1), 300 - 300, 日本語骨粗鬆症モデルラットにおけるDHEA投与は海綿骨主体の脛骨近位部における性ホルモン関連合成酵素を活性化させる
- LIPPINCOTT WILLIAMS & WILKINS, 2011年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 43(5) (5), 412 - 412, 英語Regulation of Hypertrophic Signaling Pathways to a Low-volume Resistance Exercise in Older Individuals研究発表ペーパー・要旨（国際会議）
- LIPPINCOTT WILLIAMS & WILKINS, 2011年05月, MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 43(5) (5), 299 - 299, 英語Differential Effect of Aging on Acute Hormonal Response to Low-Volume Resistance Exercise研究発表ペーパー・要旨（国際会議）
- Introduction The purpose of the present study was to elucidate the effect of unilateral lower limb suspension (ULLS) on aerobic capacity during one legged cycle exercise and whether the change in aerobic exercise capacity after ULLS with or without intensive interval training is related to the change in skeletal muscle volume Methods There were 13 young men who underwent 20 d of ULLS and were divided into 2 groups based on some physical characteristics the control group (CON, N = 7) and the trained group (TRN, N = 6) Subjects m TRN underwent interval cycle training on alternate clays during ULLS The respiratory and circulatory responses to one legged incremental cycling and muscle volume of the thigh were measured before and after 20 d of ULLS Results One legged peak oxygen uptake (VO(2peak)) and total muscle volume of the thigh in CON decreased significantly after 20 d of ULLS (-11 0 +/- 3 5% in VO(2peak) -6 1 +/- 1 8% in total muscle volume) However these parameters were maintained in TRN (+02 +/- 5 2% in VO(2peak), -1 0 +/- 1 8% in total muscle volume) Circulatory variables at rest and during submaximal exercise were unchanged in both groups after ULLS Changes in one legged VO(2peak) were significantly correlated with change in total muscle volume of the thigh in CON Conclusion Our findings indicated that peripheral adaptations after ULLS could relate to the change in aerobic exercise capacity during one legged exercise Our results also suggest that intensive interval training prevents ULLS induced deconditioning of both aerobic exercise capacity and skeletal muscle volumeAEROSPACE MEDICAL ASSOC, 2010年12月, AVIATION SPACE AND ENVIRONMENTAL MEDICINE, 81(12) (12), 1085 - 1091, 英語[査読有り]速報，短報，研究ノート等（学術雑誌）
- (一社)日本骨代謝学会, 2010年07月, 日本骨代謝学会学術集会プログラム抄録集, 28回, 251 - 251, 日本語骨粗鬆症モデルラットにおけるDHEA投与は海綿骨主体の脛骨近位部におけるアンドロゲン関連合成酵素を活性化させる
- Introduction: Atrophy Of the leg muscles is a serious consequence of disuse and Occurs with prolonged exposure to microgravity. We investigated whether intense interval training on a cycle ergometer would prevent muscle changes during unilateral lower limb suspension (ULLS). Methods: This study involved 11 health), men who were divided into 2 groups, one with training on a cycle ergometer (TRN, N = 6) and the other a control group (CON, N = 5). TRN performed intense interval training (up to 90% of maximum heart rate) on alternate days during 20 d of unloading. Maximum voluntary contraction (MVC) of the quadriceps femoris and volume of the thigh muscle were measured before and after unloading. Muscle functional magnetic resonance imaging (mfMRI), which reflects the contractile activity of the activating muscle, were acquired during submaximal dynamic knee extension exercises. Results: The MVC was significantly decreased in TRN (-11.1%) and CON (- 18.7'%). The total volume Of the thigh muscles was maintained in TRN (-0.2%); however, a significant decrease was found in CON (-6.9%). The mfMRI signal increase for submaximal exercise did not change for TRN; however, it significantly increased for CON. Conclusions: Although the power of this Study was limited, these results Suggest that training oil a cycle ergometer counteracts some of the effects of unloading and merits further study as a potential Countermeasure for muscle changes in microgravity.AEROSPACE MEDICAL ASSOC, 2009年07月, AVIATION SPACE AND ENVIRONMENTAL MEDICINE, 80(7) (7), 652 - 656, 英語[査読有り]速報，短報，研究ノート等（学術雑誌）
- 日本体力医学会, 2008年12月01日, 体力科學, 57(6) (6), 764 - 764, 日本語264. 肥満型高血糖モデルラットのインスリン抵抗性に対するDHEA摂取および運動療法の単独・併用効果(代謝,一般口演,第63回日本体力医学会大会)
- 日本体力医学会, 2008年12月01日, 体力科學, 57(6) (6), 704 - 704, 日本語144. 温浴が高齢者の左室収縮・拡張機能に及ぼす影響(呼吸・循環,一般口演,第63回日本体力医学会大会)
- (一社)日本体力医学会, 2008年12月, 体力科学, 57(6) (6), 657 - 657, 日本語一過性運動における骨格筋局所のアンドロゲン代謝活性の変動と性差
- 2008年07月, JOURNAL OF AGING AND PHYSICAL ACTIVITY, 16, S100 - S101Relationship between left ventricular diastolic function and aerobic exercise capacity in elderly men[査読有り]
- 日本体力医学会, 2007年12月01日, 体力科學, 56(6) (6), 679 - 679, 日本語202. 男性ホルモンは骨格筋の糖代謝の調節に影響する(代謝,第62回日本体力医学会大会)
- 日本体力医学会, 2007年12月01日, 体力科學, 56(6) (6), 642 - 642, 日本語128. 一過性の水中運動と陸上運動が大動脈スティフネスに及ぼす影響(呼吸・循環,第62回日本体力医学会大会)
- We compared the ventilatory and circulatory responses during 20 s of light dynamic leg and arm exercises performed separately using dominant and non-dominant limbs. Seventeen subjects performed a 20-s single-leg knee extension-flexion exercise with a load of 5% of maximal muscle strength attached to the ankle. Fifteen of the seventeen subjects also did a single-arm elbow flexion-extension exercise in which a load was attached to the wrist in the same way as in the leg exercise. Similar movements were passively performed on the subjects by experimenters to avoid the effects of central command. The magnitude of change from rest (gain) in minute ventilation during passive movement (PAS) was significantly smaller in the dominant limbs than in the non-dominant limbs, though a significant difference was not detected during voluntary exercise (VOL). In contrast, heart rate and blood pressure responses did not show any differences between the dominant and non-dominant limbs during either VOL or PAS. In conclusion, the initial ventilatory response to PAS in the dominant limbs was lower than that of the non-dominant limbs, though the ventilatory response to VOL was not. Circulatory responses were not different between the dominant and non-dominant limbs. These results suggest that peripheral neural reflex during exercise could be different between dominant and non-dominant limbs and that ventilatory response at the onset of exercise might be controlled by the dual neural modulation of central command and peripheral neural reflex, resulting in the same ventilatory response to both dominant and non-dominant limb exercise.SPRINGER, 2007年10月, EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, 101(3) (3), 347 - 358, 英語[査読有り]速報，短報，研究ノート等（学術雑誌）
- The purpose of this study was to clarify whether delayed onset muscle soreness (DOMS) and muscle damage after eccentric exercise (ECC) could affect the ventilatory and circulatory responses at the onset of exercise, and whether those effects would continue after the disappearance of DOMS. Ten males participated in this study. We measured ventilatory and circulatory responses at the onset of exercise, for the first 20 s, during knee extension-relaxation voluntary exercise (VOL) and passive movement (PAS), which was achieved by the experimenter alternatively pulling ropes connected to the subjects' ankles for the same period and frequency as during VOL. VOL and PAS were performed before, 2 days after, and 7 days after ECC. The following results were found: (1) the gain of minute ventilation at the onset of VOL at 2 days after ECC was significantly larger than that of before ECC; (2) the gain of minute ventilation at 7 days after ECC during both VOL and PAS was also enhanced significantly as compared to that of before ECC; and (3) heart rate and blood pressure responses were unchanged throughout the experimental period. In conclusion, ventilatory response at the onset of exercise is augmented during DOMS and EIMD after ECC and the enhanced ventilatory response continued after the disappearance of DOMS. It is suggested that enhanced ventilatory response during exercise after ECC is attributed to at least the changes in neural factors and that the mechanisms inducing these augmented ventilatory responses should be different during the period after ECC.SPRINGER, 2006年07月, EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, 97(5) (5), 598 - 606, 英語[査読有り]速報，短報，研究ノート等（学術雑誌）
- その他, ELSEVIER, 2018年05月, 英語Vitamins & Hormones: The Role of Dehydroepiandrosterone (DHEA) in Skeletal Muscle学術書
- その他, 体育の科学, 2013年, 日本語骨格筋由来の性ステロイドホルモンの新たな役割学術書
- その他, Journal of Physical Fitness and Sports Medicine, 2013年, 英語Exercise, nutrition, and aging in the regulation of muscle protein synthesis学術書
- Taylor & Francis Group LLC., 2011年DHEA (dehydroepiandrosterone) in Human Health and Aging. Chapter B: Prevention of Disease by DHEA, DHEA and glucose metabolism in skeletal muscle.
- 京都滋賀体育学会第153回大会, 2024年03月運動・栄養による中高齢者の肥満・糖尿病予防に向けた基礎的研究[招待有り]シンポジウム・ワークショップパネル（指名）
- 日本スポーツ栄養学会 第9回大会, 2023年09月, 日本語1型糖尿病患者における運動による遅発性低血糖発症機序と予防に向けた栄養介入[招待有り]シンポジウム・ワークショップパネル（指名）
- 第77回日本体力医学会, 2022年09月, 日本語1型糖尿病における中強度運動による遅発性低血糖の発症機序口頭発表（一般）
- 第41回日本肥満学会・第38回日本肥満症治療学会, 2021年03月, 日本語肥満を基盤とする代謝性疾患予防に向けた新たな 運動療法・栄養介入の可能性[招待有り]シンポジウム・ワークショップパネル（指名）
- 24th Annual Congress of the European College of Sports Science, 2019年07月, 英語, Prague, 国際会議The effects of exercise habits and sex on immune response in different exercise intensitiesポスター発表
- 24th Annual Congress of the European College of Sports Science, 2019年07月, 英語, Prague, 国際会議Aerobic exercise training and Dehydroepiandrosterone administration increase testicular sex steroid hormones and enhance reproductive function in obese ratsポスター発表
- 日本運動免疫研究会 スプリングセミナー, 2019年03月, 日本語, 流通科学大学, 国内会議Ⅰ型糖尿病に対して骨格筋糖代謝調節経路に着目した新規運動ガイドラインの開発公開講演，セミナー，チュートリアル，講習，講義等
- 第33回日本体力医学会 近畿地方会, 2019年02月, 日本語, 立命館大学 滋賀, 国内会議動脈硬化指標を用いて分類した中年期女性の骨密度と身体活動および栄養摂取の関係ポスター発表
- 日本調理科学会平成30年度大会, 2018年08月, 日本語, 武庫川女子大学, 国内会議ホームベーカリーによる高 β-glucan 大麦 40％含有食パンの調製と官能評価口頭発表（一般）
- 65th American College of Sports Medicine, 2018年05月, 英語, Minneapolis, MN, USA, 国際会議Resistance Training-Induced Muscle Hypertrophy is Related to Androgen Receptor Content not Intramuscular or Systemic Hormonesポスター発表
- 第72回日本体力医学会, 2017年09月, 日本語, 愛媛県松山市（松山大学）, 国内会議閉経後プレサルコペニア女性を対象とした軽負荷低反復パワートレーニングにおける骨密度変化率と脈波伝搬速度の関係ポスター発表
- 第72回日本体力医学会, 2017年09月, 日本語, 愛媛県松山市（松山大学）, 国内会議ラットにおける高強度短時間水泳トレーニングがepitrochlearis筋中及び血中SPARC濃度に及ぼす影響[招待有り]シンポジウム・ワークショップパネル（指名）
- 日本スポーツ栄養学会 第４回大会, 2017年08月, 日本語, 大妻女子大学, 国内会議アスリートのトゲドコロ摂取がレジスタンストレーニングによる 筋量・筋力に及ぼす影響口頭発表（一般）
- Cell Symposia, Exercise Metabolism, 2017年05月, 英語, Sweden, 国際会議Reduced blood insulin stimulates resistance exercise-induced AMPK pathway activation concomitant with AMPK alpha Ser 485/491 inhibitory phosphorylation in rat skeletal muscleポスター発表
- The Integrative Biology of Exercise VII, 2016年11月, 英語, Phoenix, Arizona, USA, 国際会議The effect of chronic resistance trainingon myonuclear abundance in OLETF rat skeletal muscleポスター発表
- The Integrative Biology of Exercise VII, 2016年11月, 英語, Phoenix, Arizona, USA, 国際会議Resistance training improves insulin-stimulated Akt activation, related to APPL1 expression in type 2 diabetic rat skeletal muscleポスター発表
- 第71回日本体力医学会, 2016年09月, 日本語, 岩手, 国内会議２型糖尿病ラットの有酸素性トレーニングによる動脈硬化の改善に動脈血管のadropin産生の増大が影響する口頭発表（一般）
- 第71回日本体力医学会, 2016年09月, 日本語, 岩手, 国内会議高齢期における運動トレーニングが筋局所の性ホルモン産生経路に及ぼす影響口頭発表（一般）
- 第71回日本体力医学会, 2016年09月, 日本語, 岩手, 国内会議インスリン分泌欠乏は筋収縮性のAMPK-Thr172のリン酸化を高める口頭発表（一般）
- 第16回抗加齢医学会大会, 2016年06月, 日本語, 横浜, 国内会議中高齢者の動脈硬化改善に必要な有酸素性トレーニング期間と血管拡張物質産生に関する経時的検討口頭発表（一般）
- 63th Annual Meeting, American College of Sports Medicine, 2016年06月, 英語, Boston, USA, 国際会議Serum Adropin Level Is Associated With Arterial Stiffness And Cardiorespiratory Fitness In Older Adultsポスター発表
- 63th Annual Meeting, American College of Sports Medicine, 2016年06月, 英語, Boston, USA, 国際会議Resistance Exercise-induced mTORC1-signal Activation Is Not Impaired in Skeletal-muscle of STZ-induced Type 1 Diabetic Ratsポスター発表
- 63th Annual Meeting, American College of Sports Medicine, 2016年06月, 英語, Boston, USA, 国際会議Muscular Sex Steroid Hormone Contributes To Resistance Training-induced Skeletal Muscle Hypertrophy In Obese Rats口頭発表（一般）
- 第70回日本栄養・食糧学会大会, 2016年05月, 日本語, 武庫川女子大学, 国内会議肥満モデルラットのDHEA長期摂取は動脈組織のapelin産生増大を介してnitric oxide産生調節に影響する口頭発表（一般）
- 第70回日本栄養・食糧学会大会, 2016年05月, 日本語, 武庫川女子大学, 国内会議長期的なクロレラ摂取は無酸素性運動能力と骨格筋の解糖系を亢進させる口頭発表（一般）
- 第70回日本栄養・食糧学会大会, 2016年05月, 日本語, 武庫川女子大学, 国内会議中高齢者のトゲドコロ長期摂取による動脈硬化度への影響口頭発表（一般）
- 第59回日本糖尿病学会年次学術集会, 2016年05月, 日本語, 国立京都国際会館、みやこめっせ京都, 国内会議単回レジスタンス運動の伴うAMPKのリン酸化及びGLUT4のトランスロケーションは一型糖尿病モデルラットの骨格筋で減弱しない口頭発表（一般）
- 第59回日本糖尿病学会年次学術集会, 2016年05月, 日本語, 国立京都国際会館, 国内会議1型糖尿病における運動による持続的な糖代謝亢進が遅発性低血糖を引き起こす口頭発表（一般）
- 第３８回日本分子生物学会年会, 2015年12月, 日本語, 神戸ポートアイランド, 国内会議In vivo骨格筋最大収縮に伴う細胞内糖取り込み制御因子の応答ポスター発表
- 第３８回日本分子生物学会年会, 2015年12月, 日本語, 神戸ポートアイランド, 国内会議3T3-L1細胞において、dehydroepiandrosteroneはAMPキナーゼの活性化を介して脂肪細胞分化を抑制するポスター発表
- 20th Annual Congress of the European College of Sport Science, 2015年06月, 英語, Malmo, Sweden, 国際会議The relationship between intramyocellular or extramyocellular lipid contents and cardiovascular disease risks in different cardiorespiratory fitness levels口頭発表（一般）
- American Diabetes Association75th scientific sessions, 2015年06月, 英語, American Diabetes Association, ボストン, 国際会議Delayed onset of hypoglycemia after an acute bout of exercise is associated with prolonged muscle GLUT-4 translocation in type 1 diabetes.口頭発表（一般）
- 62th ACSM Annual Meeting, 2015年05月, 英語, San Diego, USA, 国際会議Serum CTRP9 level is associated with arterial stiffness and cardiorespiratory fitnessポスター発表
- 12th Asian Congress of Nutrition, 2015年05月, 英語, Yokohama, Japan, 国際会議Chronic chlorella intake and high intensity intermittent exercise enhance exercise capacity and muscular glycolytic enzyme activity in rats.ポスター発表
- 日本体力医学会第69回大会, 2014年09月, 日本語, 日本体力医学会, 長崎, 国内会議有酸素性トレーニングによる動脈硬化改善と筋細胞内・外脂肪含有量との関係.口頭発表（一般）
- 日本体力医学会第69回大会, 2014年09月, 日本語, 日本体力医学会, 長崎, 国内会議肥満モデルラットにおける運動およびDHEA投与が骨格筋の脂質代謝制御転写因子に与える影響.口頭発表（一般）
- 日本体力医学会第69回大会, 2014年09月, 日本語, 日本体力医学会, 長崎, 国内会議中高齢者における有酸素性トレーニングが血中adropin濃度に及ぼす影響.口頭発表（一般）
- 日本体力医学会第69回大会, 2014年09月, 日本語, 日本体力医学会, 長崎, 国内会議高齢者の一過性レジスタンス運動による骨格筋性ステロイド代謝応答はトレーニングによる筋量・筋力増大に関連する.口頭発表（一般）
- 日本体力医学会第69回大会, 2014年09月, 日本語, 日本体力医学会, 長崎, 国内会議血中C1qレベルは高齢者のレジスタンストレーニングによる筋肥大効果に関連する.口頭発表（一般）
- 日本運動生理学会第22回大会, 2014年07月, 日本語, 日本運動生理学会, 岡山, 国内会議肥満ラットの運動トレーニングによる動脈血管拡張因子の改善に動脈Apelin産生の増大が関与する.口頭発表（一般）
- 日本運動生理学会第22回大会, 2014年07月, 日本語, 日本運動生理学会, 岡山, 国内会議体力レベルの違いが筋細胞内・外脂肪含有量と動脈硬化指数の関係に及ぼす影響.口頭発表（一般）
- 日本運動生理学会第22回大会, 2014年07月, 日本語, 日本運動生理学会, 岡山, 国内会議血中C1qレベルは加齢に伴う筋量・筋力の変化に関連する.口頭発表（一般）
- 日本運動生理学会第22回大会, 2014年07月, 日本語, 日本運動生理学会, 岡山, 国内会議運動およびジオスゲニン摂取は骨格筋内の性ホルモン増大を介してインスリン抵抗性を改善する.口頭発表（一般）
- 日本運動生理学会第22回大会, 2014年07月, 日本語, 日本運動生理学会, 岡山, 国内会議運動およびDHEA投与は肥満モデルラットの骨格筋脂質代謝制御転写因子の発現を増大させる.口頭発表（一般）
- 19th Annual Congress, 2014年06月, 英語, European College of Sport Science, オランダ, 国際会議Effects of intramyocellular and extramyocellular lipid contents on arterial stiffness.ポスター発表
- 19th Annual Congress, 2014年06月, 英語, European College of Sport Science, オランダ, 国際会議Effect of central arterial stiffness on acute stretching exercise in young men.ポスター発表
- 61st Annual Congress, 2014年05月, 英語, American College of Sport Medicine, オーランド, 国際会議Reduction of arterial stiffness by exercise training is associated with increasing plasma apelin level.ポスター発表
- 61st Annual Congress, 2014年05月, 英語, American College of Sport Medicine, オーランド, 国際会議Exercise order of concurrent endurance and resistance exercise modifies mTOR signaling in rat skeletal muscle.ポスター発表
- 61st Annual Congress, 2014年05月, 英語, American College of Sport Medicine, オーランド, 国際会議Effects of the menstrual cycle on the metabolomic profiles during endurance exercise in female athletes.ポスター発表
- 61st Annual Congress, 2014年05月, 英語, American College of Sport Medicine, オーランド, 国際会議Effects of cardiorespiratory fitness level, age, and sex on intramyocellular and extramyocellular lipid contents.ポスター発表
- 61st Annual Congress, 2014年05月, 英語, American College of Sport Medicine, オーランド, 国際会議Dioscorea-induced increase of sex steroid hormone improve hyperglycemia in type 2 diabetic rats.ポスター発表
- 61st Annual Congress, 2014年05月, 英語, American College of Sport Medicine, オーランド, 国際会議DHEA administration-induced increase in adiponectin secretion is associated with muscular PPAR-alpha expression in obese rats.ポスター発表
- 61st Annual Congress, 2014年05月, 英語, American College of Sport Medicine, オーランド, 国際会議Antioxidant inhibits resistance exercise induced phosphorylation of p70s6K in rats.ポスター発表
- 61st Annual Congress, 2014年05月, 英語, American College of Sport Medicine, オーランド, 国際会議Ageing-induced reduction in muscle cross-sectional area is associated with serum C1q concentration.ポスター発表
- 日本体力医学会第68回大会, 2013年09月, 日本語, 日本体力医学会, 東京, 国内会議有酸素性運動に伴う筋内アミノ酸濃度とmTORC1の応答.口頭発表（一般）
- 日本体力医学会第68回大会, 2013年09月, 日本語, 日本体力医学会, 東京, 国内会議中高齢者における有酸素性トレーニングが血中apelin濃度に及ぼす影響.口頭発表（一般）
- 日本体力医学会第68回大会, 2013年09月, 日本語, 日本体力医学会, 東京, 国内会議持久性トレーニングによる血中Irishin濃度の変化と内臓脂肪量減少量の関係.口頭発表（一般）
- 日本体力医学会第68回大会, 2013年09月, 日本語, 日本体力医学会, 東京, 国内会議高齢者のレジスタンストレーニングによる骨格筋内性ステロイド合成の改善が筋量増大に関連する.口頭発表（一般）
- 日本体力医学会第68回大会, 2013年09月, 日本語, 日本体力医学会, 東京, 国内会議筋細胞内脂肪含有量と動脈硬化指数の関係.口頭発表（一般）
- 日本体力医学会第68回大会, 2013年09月, 日本語, 日本体力医学会, 東京, 国内会議レジスタンス運動と有酸素性運動の実施順が筋タンパク質同化応答を決定する.口頭発表（一般）
- 日本体育学会第64回大会, 2013年07月, 日本語, 日本体育学会, 滋賀, 国内会議持久能鍛錬者と非鍛錬者の異なる運動負荷における性ホルモン濃度変化の比較.口頭発表（一般）
- 日本体育学会第64回大会, 2013年07月, 日本語, 日本体育学会, 滋賀, 国内会議高齢者を対象とした筋力トレーニングにおける強度の違いが筋機能および骨格筋量に及ぼす影響.口頭発表（一般）
- 日本体育学会第64回大会, 2013年07月, 日本語, 日本体育学会, 滋賀, 国内会議アスリートの競技種目の特性に影響する骨格筋タンパクの検索.口頭発表（一般）
- 18th Annual Congress, 2013年06月, 英語, European College of Sport Science, スペイン, 国際会議The cross-sectional study of the relationships among household physical activity, locomotive physical activity and metabolic syndrome risks factors.ポスター発表
- 18th Annual Congress, 2013年06月, 英語, European College of Sport Science, スペイン, 国際会議Combination of α-Glycerophosphocholine （α-GPC） intake and aerobic exercise improves cognitive functions in healthy older women.ポスター発表
- 18th Annual Congress, 2013年06月, 英語, European College of Sport Science, スペイン, 国際会議Combination of α-Glycerophosphocholine （α-GPC） intake and aerobic exercise enhances growth hormone secretion and fat metabolism in young adults.ポスター発表
- 日本栄養・食糧学会第67回大会, 2013年05月, 日本語, 日本栄養・食糧学会, 愛知, 国内会議コリンサプリメント摂取と運動の併用が成長ホルモン分泌および脂質代謝に及ぼす影響.口頭発表（一般）
- 日本栄養・食糧学会第67回大会, 2013年05月, 日本語, 日本栄養・食糧学会, 愛知, 国内会議ウルソール酸はレジスタンス運動後のラット骨格筋Aktリン酸化を亢進させる.口頭発表（一般）
- 日本栄養・食糧学会第67回大会, 2013年05月, 日本語, 日本栄養・食糧学会, 愛知, 国内会議1型糖尿病モデルラットにおける単回のジオスゲニン投与による骨格筋糖代謝活性と血糖値に及ぼす影響.口頭発表（一般）
- Annual Congress 2013, 2013年04月, 英語, Experimental Biology, ボストン, 国際会議Effect of oligomerized polyphenol supplementation on lipid metabolism during exercise in healthy men.ポスター発表
- Annual Congress 2013, 2013年04月, 英語, Experimental Biology, ボストン, 国際会議Diosgenin administration improve hyperglycemia through the activation of muscular glucose metabolism.ポスター発表
- 第25回日本トレーニング学会, 2012年11月性ステロイドホルモン投与と運動トレーニングが高ショ糖食摂取による肥満ラットに及ぼす影響
- 日本体力医学会第67回大会, 2012年09月, 日本語, 日本体力医学会, 岐阜, 国内会議運動トレーニングによる性ステロイドホルモン増加は2型糖尿病モデルラットの骨格筋糖代謝を亢進させる.口頭発表（一般）
- 日本体力医学会第67回大会, 2012年09月, 日本語, 日本体力医学会, 岐阜, 国内会議レジスタンス運動後のロイシン高配合必須アミノ酸混合物摂取が筋タンパク質分解の制御に及ぼす影響.口頭発表（一般）
- 日本体育学会第63回大会, 2012年08月, 日本語, 日本体育学会, 神奈川, 国内会議異なるタンパク質摂取が運動時の脂質代謝に及ぼす影響.口頭発表（一般）
- 日本体育学会第63回大会, 2012年08月, 日本語, 日本体育学会, 神奈川, 国内会議ヒト骨格筋に性ステロイドホルモン合成酵素が発現する－加齢による影響－.口頭発表（一般）
- 17th Annual Congress, 2012年07月, 英語, European College of Sport Science, ベルギー, 国際会議Effect of reduced training frequency on muscle mass and serum testosterone in older men.ポスター発表
- 59th Annual Congress, 2012年05月, 英語, American College of Sport Medicine, サンフランシスコ, 国際会議Low-intensity resistance training using an elastic band improves muscle mass and function in older person.ポスター発表
- 59th Annual Congress, 2012年05月, 英語, American College of Sport Medicine, サンフランシスコ, 国際会議Exercise training-induced improvement of impaired muscle glucose metabolism is associated with muscular sex steroid hormone level in Zucker fatty diabetes rats.ポスター発表
- 日本体力医学会第66回大会, 2011年09月, 日本語, 日本体力医学会, 山口, 国内会議一過性レジスタンス運動時の筋タンパク合成関連シグナル経路調節―加齢の影響.口頭発表（一般）
- 日本体力医学会第66回大会, 2011年09月, 日本語, 日本体力医学会, 山口, 国内会議コリンサプリメントが成長ホルモン分泌と脂質代謝に与える影響.口頭発表（一般）
- International Conference on Nutrition and Aging6th Congress, 2011年09月, 英語, Nutrition and Aging, 東京, 国際会議The role of nutrient intake in Sarcopenia prevention.[招待有り]シンポジウム・ワークショップパネル（指名）
- 日本運動生理学会第19回大会, 2011年08月, 日本語, 日本運動生理学会, 徳島, 国内会議運動による骨格筋内の性ホルモン増大がインスリン抵抗性改善に関連するか？口頭発表（一般）
- 16th Annual Congress, 2011年06月, 英語, European College of Sport Science, イギリス, 国際会議Increased muscular dehydroepiandorosterone levels are associated with improved hyperglycemia in obese rats.ポスター発表
- 58th Annual Congress, 2011年05月, 英語, American College of Sport Medicine, デンバー, 国際会議Regulation of hypertrophic signaling pathways to a low-volume resistance exercise in older individuals.ポスター発表
- 58th Annual Congress, 2011年05月, 英語, American College of Sport Medicine, デンバー, 国際会議Differential effect of aging on acute hormonal response to resistance exercise.ポスター発表
- International Sportology Conference1st Congress, 2011年03月, 英語, Sportology Society, 東京, 国際会議DHEA administration and exercise training improves insulin resistance in obese rats.ポスター発表
- 日本テストステロン研究会 第1回大会, 2010年11月, 日本語, 東京, 国内会議DHEA と骨格筋糖代謝活性.[招待有り]シンポジウム・ワークショップパネル（指名）
- 第10回Men’s Health 医学会, 2010年11月Dehydroepiandrosteron (DHEA) と骨格筋糖代謝
- 第65回大会, 2010年09月, 日本語, 日本体力医学会, 千葉, 国内会議DHEA投与が1型糖尿病モデルラットにおける血糖値および骨格筋糖代謝調節経路に及ぼす影響.ポスター発表
- Intersociety meeting 2010, 2010年08月, 英語, American Physiological Society, デンバー, 国際会議The effect of rice bran arabinoxylan compound on glucose metabolism in skeletal muscle.ポスター発表
- 日本抗加齢医学会 第10回大会, 2010年06月, 日本語, 京都, 国内会議DHEA（Dehydroepiandrosteron） と骨格筋糖代謝活性.[招待有り]シンポジウム・ワークショップパネル（指名）
- Nutrition and Integrative Medicine18th Congress, 2010年05月, 英語, Nutrition and Integrative Medicine, 北海道, 国際会議Effect of oligomerised polyphenol supplementation on lipid metabolism and endothelial function among young subjects.[招待有り]口頭発表（招待・特別）
- 日本体力医学会第63回大会, 2008年09月, 日本語, 日本体力医学会, 大分, 国内会議肥満型高血糖モデルラットのインスリン抵抗性に対するDHEA摂取および運動療法の単独・併用効果.ポスター発表
- Intersociety meeting 2008, 2008年09月, 英語, American Physiological Society, ボストン, 国際会議Exercise stimulates local bioactive androgen metabolism in skeletal muscle.ポスター発表
- 日本運動生理学会 第16回大会, 2008年08月, 日本語, 奈良, 国内会議Does DHEA affect glucose metabolism in skeletal muscle?公開講演，セミナー，チュートリアル，講習，講義等
- 13th Annual Congress, 2008年07月, 英語, European College of Sport Science, ポルトガル, 国際会議DHEA activates the glucose metabolism-related signalling pathway in skeletal muscle.ポスター発表
- 第62回大会, 2007年09月, 日本語, 日本体力医学会, 秋田, 国内会議男性ホルモンは骨格筋の糖代謝に影響する.ポスター発表
- 第41回日本肥満学会・第38回日本肥満症治療学会肥満を基盤とする代謝性疾患予防に向けた新たな運動療法・栄養介入の可能性[招待有り]シンポジウム・ワークショップパネル（指名）
- 第41回日本肥満学会・第38回日本肥満症治療学会, 日本語肥満を基盤とする代謝生疾患予防に向けた新たな運動療法・栄養介入の可能性[招待有り]シンポジウム・ワークショップパネル（指名）
■ 共同研究・競争的資金等の研究課題
- 日本学術振興会, 科学研究費助成事業, 基盤研究(B), 神戸大学, 2024年04月01日 - 2028年03月31日循環・呼吸両調節システムとの協働則を踏まえた純粋なバランス調節機能評価指標の確立
- 日本学術振興会, 科学研究費助成事業, 基盤研究(C), 四天王寺大学, 2024年04月01日 - 2028年03月31日月経周期に伴う心身不調と睡眠習慣との関連：精神神経内分泌免疫動態からの探索
- 日本学術振興会, 科学研究費助成事業, 基盤研究(B), 神戸大学, 2024年04月 - 2028年03月中高齢者のトゲドコロイモ摂取における機能性とその効能の解明
- 日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 2023年04月01日 - 2027年03月31日心理的ストレスによる不眠予測およびセルフモニタリングツールの開発とその効果検証
- 日本学術振興会, 科学研究費助成事業, 国際共同研究加速基金(国際共同研究強化), 神戸大学, 2024年07月 - 2026年07月, 研究代表者中高齢女性特有の疾患における新たな予防策の開発
- 日本学術振興会, 科学研究費助成事業, 挑戦的研究(萌芽), 神戸大学, 2023年06月30日 - 2026年03月31日高度情報化社会に必要な科学関連情報評価能力の育成を促進する教師教育プログラム開発
- 日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 神戸大学, 2022年04月01日 - 2025年03月31日感情調整と信頼の加齢変化と社会的つながりに関する縦断研究
- 日本学術振興会, 科学研究費助成事業 基盤研究(C), 基盤研究(C), 神戸大学, 2021年04月01日 - 2024年03月31日糖尿病予防に向けた新規骨格筋糖代謝調節経路の解明本研究では、運動トレーニングによる2型糖尿病予防に向けた新規骨格筋糖代謝調節経路を確立するために、細胞培養実験を用いて以下の点を解明することを目的とする。 「新規の骨格筋糖代謝調節システムの同定：肥満や2型糖尿病に由来する炎症性サイトカインや酸化ストレスの増加によるOSMの低下が、骨格筋糖代謝調節経路の活性化抑制に関与することを示すとともに、性ステロイドホルモン投与によるOSMの増加が、骨格筋糖代謝調節経路を亢進するか否かを確かめる。」 本年度は課題1を行い、骨格筋培養細胞（C2C12）を対象とし、抗炎症作用等を有するオンコスタチンM(OSM)を遺伝子導入によりノックダウンさせ、骨格筋糖代謝調節経路に及ぼす影響を検討した。遺伝子導入は、OSM抑制のためのshRNAプラスミドベクターを骨格筋細胞に導入し、インスリンやDHEA、DHEAを増加させる栄養成分であるトゲドコロの粉末を骨格筋細胞に添加し、24時間後細胞を回収し、Western Blotting法により、骨格筋の糖代謝調節経路活性及び糖取り込みを検討した。 OSMを欠損した骨格筋細胞において、インスリンやDHEAを添加すると、骨格筋糖代謝調節経路活性や骨格筋による糖取り込みは通常の骨格筋細胞より有意に亢進し、OSMの増加に伴う炎症性サイトカインの増加が、インスリン抵抗性や骨格筋糖代謝活性の不活性化に関与している可能性が示唆された。 また、DHEAやジオスゲニンの投与は、先行研究より、抗炎症作用があることから、本研究においても、添加24時間後の通常の骨格筋細胞においてもOSMの発現が抑制されることが明らかとなった。
- 日本学術振興会, 科学研究費助成事業 挑戦的研究(萌芽), 挑戦的研究(萌芽), 神戸大学, 2020年07月30日 - 2023年03月31日高度情報化社会に求められる科学関連情報評価能力の育成手法と実践モデルの開発本研究では，近年の学習科学で国際的に着目されている「エピステミック学習理論」を応用し，高度情報化社会に対応した科学関連情報評価能力の理論化を行い，その育成手法と実践モデルの開発に取り組む．研究方法については，この研究目的の達成のために，学際的な共同研究体制を組織している． 2021年度は，科学関連情報評価能力の育成手法と実践モデルの開発，研究実践フィールドにおける実証実験を実施した．2021年度の研究実績については，次の通りであった．（1）前年度の研究から得られた科学関連情報評価に必要な資質・能力・態度に基づいて，科学関連情報評価能力のための教育目的を設定した．（2）科学関連情報評価能力に関する教育内容を開発するとともに，学習科学の研究成果を応用した教育方法を開発した．（3）さらに，量的分析および質的分析の観点から，科学関連情報評価能力に特化した教育評価を開発した．（4）これらの科学関連情報評価能力に特化した教育目的・方法・内容・評価を統合した実践モデルの実証実験を実施し，効果測定のためのデータを収集・分析した．なお，実証実験については，新型コロナウイルス感染症拡大の影響に応じて，その実施方法について検討を行った．2021年度の研究成果の発表に関しては，教育システム情報学会誌，The 19th Biennial EARLI Conference，日本教育工学会2022年春季全国大会，日本科学教育学会第45回年会，日本理科教育学会第71回全国大会において研究成果の一部を発表した．また， International Society of Learning Sciences Annual Meeting 2022に採択された．この他，研究成果の一部を公表した図書が2022年6月に刊行予定である．
- 日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 神戸大学, 2019年04月01日 - 2022年03月31日エンド・オブ・ライフにおける感情調整機能の機序と役割感情調整機能は，自立した生活が困難となる人生の最終段階である約10年間(エンド・オブ・ライフ)の心理的安寧や生活の質(QOL)を左右する最も重要な機能の一つである。身体的，認知的機能の多くは加齢とともに低下するが，感情のコントロールを担う感情調整機能は加齢による低下がみられずむしろ向上する。 高齢期の感情調整機能は低下しないのか?を明らかにするための実験と，感情調整機能が人間関係の喪失，社会的役割の喪失，健康の喪失にどのように影響するのか?を明らかにするための調査を実施した。 実験では，65歳以上の高齢者100名（平均年齢71.74歳, SD=3.85）を対象とし，感情調整機能の測定，認知機能の検査（WAIS -Ⅳ），処理される情報の質の評価として自伝的記憶課題，遺伝子解析のための唾液のサンプリングを行った。その結果，感情調整と種々の認知機能検査結果との間に関連性は認められなかった。一方で，普段の生活の中でよく想起される記憶の感情価，重要度，鮮明度といった指標と感情調整には関連性が認められた。これらの結果は，認知機能のパフォーマンスではなく，どのような情報を思い出し処理するかといった情報の質が感情調整と関連していることを示唆している。 調査では， 20-70代の936名を対象とし，感情調整，社会的つながり，孤独感の測定を実施した。その結果，男性よりも女性の方が感情調整を頻繁におこなうこと，社会的つながりが多い人は感情調整の頻度が高いこと，感情調整をおこなうことで孤独感が低下すること，加齢に伴い感情調整の頻度は低下をすることが示唆された。
- 公益財団法人 戸部眞紀財団, 研究助成, 2020年10月 - 2021年09月, 研究代表者女子大学生の各性周期における運動刺激に対する生理・生化学的応答の解明
- 日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 立命館大学, 2017年04月01日 - 2021年03月31日ビタミンDと運動併用による筋肥大メカニズムの解明と新たなサルコペニア予防法の開発2019年度は2つの実験課題を実施した。 研究課題①は、健常な60‐80歳の高齢男女99名（年齢：68.1±5.1歳）を対象とした。二重エネルギーX線吸収法を用いて、全身、四肢の除脂肪量を評価した。加えて、筋力指標として、握力、脚伸展力を測定した。安静状態にて血液サンプルを採取し、血中25(OH)D濃度を評価した。更に、習慣的な食事摂取量（BDHQ）と身体活動量（IPAQ）を収集した。 被験者99名の血中25(OH)D濃度の平均値は88.3±33.3 nmol/lで、血中25(OH)D濃度が不足している（＜50 nmol/l）被験者は11％（9名）であった。四肢非脂肪量の平均値は17.1±3.6㎏で、握力の平均値は29.2±7.2kgだった。血中25(OH)D濃度は、全身非脂肪量（β＝0.077,p＝0.066）、脚伸展力（β＝-0.015,p＝0.848）、握力（β＝0,074,p＝0.120）とは有意な関連が認められなかったものの、四肢非脂肪量（β＝0.097,p＝0.025）と統計的に有意な関連が認められた。 本研究においては、血中25(OH)D濃度は高値（平均88.3±33.3 nmol/l）であった。以上のことから、筋力に対する機能を発揮するには、一定以上の血中25(OH)D濃度があれば可能であることが考えられ、本研究においても、ほとんどの被験者が必要な血中25(OH)D濃度を満たしていた（平均88.3±33.3 nmol/l, ＜50 nmol/l（不足）の被験者＝9名）ため、筋力に対する濃度依存的な関係が認められなかった可能性が考えられる。 研究課題②は、健常な若年男性を被験者として研究を実施した。一過性のレジスタンス運動を実施し、その前後において筋生検により筋サンプルを採取することで、運動前後における筋細胞内でのビタミンD受容体や筋たんぱく質合成に関わるシグナルタンパク質の発現を比較・検討することを目的とした。研究課題②は次年度も引き続き継続し、結果をまとめる予定である。
- 日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 筑波大学, 2015年04月01日 - 2018年03月31日本研究は，運動により変化する性ステロイドホルモンが動脈硬化度の低下効果に貢献するのかを明らかにするために，性ステロイドホルモン合成酵素の合成阻害剤を用いて直接的に運動効果に影響するかについて検討した。肥満型糖尿病モデルラットを用いて、8週間の有酸素性トレーニングによって動脈硬化度は低下したが、運動とともに活性化型テストステロンであるDHTやエストロゲンであるE2の合成酵素の抑制剤により性ステロイドホルモンの分泌を抑制したが、運動による動脈硬化度の低下効果には影響を及ぼさなかった。本研究結果から，習慣的な有酸素性運動による動脈硬化度の低下に性ステロイドホルモンは関与しない可能性が考えられる。
- 日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 立命館大学, 2014年04月01日 - 2017年03月31日本研究は，中高齢者の有酸素性トレーニングによりマイオカインの分泌が動脈硬化度の低下に関与するか否かについて検討した。健常な中高齢者男女を対象に，60-70%VO2peak運動強度の自転車運動(週3日，45分間)を8週間実施した。運動トレーニングにより動脈硬化度は有意に低下し，マイオカインの１つであるapelinおよびirisinの血中濃度は有意に増加した。これらのマイオカインと動脈硬化度の変化率との間には負の相関関係が認められた。以上の結果から，中高齢者の有酸素性トレーニングによる動脈硬化度改善の機序にマイオカインの１つであるapelinやirisinが関与する可能性が示唆された。
- 日本学術振興会, 科学研究費助成事業 挑戦的萌芽研究, 挑戦的萌芽研究, 2014年04月01日 - 2017年03月31日活性酸素種および活性酸素種とレジスタンス運動の組み合わせが筋タンパク質合成に関与するシグナル伝達応答に及ぼす影響について検討した。その結果、活性酸素種の投与は、安静時の骨格筋におけるmTORC1を活性化することがわかった。しかしながら、活性酸素種投与はレジスタンス運動により惹起されるmTORC1活性化を亢進させなかった。
- 日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 立命館大学, 2013年04月01日 - 2017年03月31日実験動物の骨格筋を対象とした試験管内(in vitro)の実験から、ヒトの運動トレーニング実験まで行い、異なる強度の運動トレーニングが、大腸がんの発症予防に有効である機序を明らかにした。特に高強度・短時間・間欠的運動トレーニングは、運動強度依存性の活動筋中のAMPK活性を上昇させ、それがPGC1αの発現量を高め、さらにそれが、大腸における前がん細胞であるACFのアポトーシスを誘導するSPARC発現量を高め、それにより血中のSPARC濃度が増加することにより将来の大腸がん発症リスクを低下させることが明らかになった。
- 公益財団法人石本記念デサントスポーツ科学振興財団, 2016年04月 - 2017年03月, 研究代表者1型糖尿病における運動誘発性遅発低血糖の発生機序の解明〜1型糖尿病患者の新規運動ガイドライン作成に向けて〜
- 公益財団法人日本糖尿病財団コストコ研究助成, 2016年04月 - 2017年03月, 研究代表者1 型糖尿病における運動による遅発性低血糖発症のメカニズムの解明 —遺伝子およびタンパク質網羅解析による原因究明—
- 日本学術振興会, 科学研究費助成事業 挑戦的萌芽研究, 挑戦的萌芽研究, 東京大学, 2013年04月01日 - 2015年03月31日骨格筋量調節に関わる細胞外マトリックス構成タンパク質、細胞接着分子、マトリックスメタロプロテアーゼ（MMPs）について、レジスタンス運動（RE）および加齢の影響を検討した。REを繰り返し、タンパク質合成および筋肥大応答が停滞したラット骨格筋において、インテグリン発現量の増加と一過性REに対するMMP-2, 9活性化応答の低下が観察された。高齢男性の骨格筋において若年男性に比べてインテグリン発現量が多く、MMP-2, 9の活性が低下していることがわかった。これらの結果は、インテグリンとMMPs活性の変化が運動による骨格筋量の調節において重要な役割を果たしている可能性を示唆する。
- 日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 筑波大学, 2012年04月01日 - 2015年03月31日本研究はヒト骨格筋内性ステロイドホルモンの合成酵素発現および組織濃度の検討と加齢および高齢期のレジスタンストレーニングによる発現・分泌変動が筋量・筋力の増加に関連するかを検討した。ヒトの筋生検により、骨格筋内の性ステロイドホルモン合成酵素(3β-HSD, 17β-HSD, 5α-reductase)発現が認められた。また、これらの発現および性ステロイドホルモン濃度は加齢により低下したが、レジスタンストレーニングにより改善し、骨格筋内の性ステロイドホルモン濃度と筋量・筋力との間に有意な正相関が認められた。骨格筋内の性ステロイドホルモンは高齢者の筋量・筋力の維持・増進に関与する可能性が示唆された。
- 笹川科学研究助成, 2012年04月 - 2013年03月運動によるヒト骨格筋の性ステロイドホルモン合成の変動競争的資金
- ヤマハ発動機スポーツ研究助成, 2012年04月 - 2013年03月ヒト骨格筋における運動、食事が性ステロイドホルモン合成に与える影響競争的資金
- 日本学術振興会, 科学研究費助成事業 若手研究(B), 若手研究(B), 立命館大学, 2011年 - 2013年研究課題１では、1型糖尿病モデルラットにおいて急性のジオスゲニン（性ステロイドホルモン前駆体のDHEAに化学構造が類似している栄養成分）を投与した結果、投与後90分後に有意に高血糖が改善し、骨格筋糖代謝調節経路の亢進が認められた。ジオスゲニン投与により骨格筋内の性ステロイドホルモン濃度が増加し、骨格筋糖代謝活性を亢進し、高血糖を改善することが明らかとなった。 研究課題２において、2型糖尿病モデルラットに８週間ジオスゲニンを投与した結果、骨格筋糖代謝活性は亢進し、空腹時血糖、インスリン濃度、さらにインスリン感受性においても有意に改善し、ジオスゲニン投与が２型糖尿病を改善する可能性を見いだした。