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HASUNUMA Tomohisa
Engineering Biology Research Center
Professor

Researcher basic information

■ Research news
  • 06 May 2021, Microalgae biofuels: Changing carbohydrates into lipids
    Link to Kobe Univ. HP
  • 10 Mar. 2020, Successful method yielding high rate of D-lactate using cyanobacteria could revolutionize bioplastic production
    Link to Kobe Univ. HP
  • 17 Jan. 2020, Novel protein positioning technique improves functionality of yeast cells -Expected improvements in cell surface engineering across a variety of fields-
    Link to Kobe Univ. HP
  • 13 Dec. 2019, Success in metabolically engineering marine algae to synthesize valuable antioxidant astaxanthin
    Link to Kobe Univ. HP
  • 06 Sep. 2019, Fe metabolic engineering method succeeds in producing 1,2,4-butanetriol sustainably from biomass
    Link to Kobe Univ. HP
  • 06 Jun. 2018, World’s most efficient production of succinate from carbon dioxide
    Link to Kobe Univ. HP
  • 17 Apr. 2017, Making oil from algae ? towards more efficient biofuels
    Link to Kobe Univ. HP
■ Research Areas
  • Life sciences / Cell biology
  • Life sciences / Applied biochemistry
  • Life sciences / Applied microbiology
  • Manufacturing technology (mechanical, electrical/electronic, chemical engineering) / Applied biofunctional and bioprocess engineering

Research activity information

■ Award
  • Oct. 2022 神戸大学, 令和4年度 学長表彰（財務貢献者） 神戸大学
    蓮沼誠久

  • Oct. 2021 神戸大学, 令和3年度 学長表彰（財務貢献者）
    蓮沼誠久

  • Oct. 2020 神戸大学, 令和2年度 学長表彰（財務貢献者）
    蓮沼誠久

  • Oct. 2019 神戸大学, 第11回学長表彰（財務貢献者）
    蓮沼 誠久

  • Oct. 2018 神戸大学, 第10回学長表彰（財務貢献者）
    蓮沼 誠久

  • Oct. 2017 神戸大学, 第9回学長表彰（財務貢献者）
    蓮沼 誠久

  • Sep. 2014 The Society for Biotechnology, Japan, Biotechnology Encouragement Prize, 代謝プロファイリングに基づく微生物育種技術の開発と応用
    HASUNUMA Tomohisa

  • Oct. 2013 Japan Bioindustry Association, Fermentation and Metabolism Research Prize, システムバイオロジー解析に基づく高機能型酵母創製とセルロースエタノール高生産技術開発への応用
    HASUNUMA Tomohisa

■ Paper
  • Yuji Haraguchi, Yuichi Kato, Kosuke Inabe, Akihiko Kondo, Tomohisa Hasunuma, Tatsuya Shimizu
    Springer Science and Business Media LLC, Jun. 2023, Archives of Microbiology, 205(7) (7), English
    [Refereed]
    Scientific journal

  • Yuichi Kato, Kosuke Inabe, Yuji Haraguchi, Tatsuya Shimizu, Akihiko Kondo, Tomohisa Hasunuma
    Abstract l-Lactate is a major waste compound in cultured animal cells. To develop a sustainable animal cell culture system, we aimed to study the consumption of l-lactate using a photosynthetic microorganism. As genes involved in l-lactate utilization were not found in most cyanobacteria and microalgae, we introduced the NAD-independent l-lactate dehydrogenase gene from Escherichia coli (lldD) into Synechococcus sp. PCC 7002. The lldD-expressing strain consumed l-lactate added to basal medium. This consumption was accelerated by expression of a lactate permease gene from E. coli (lldP) and an increase in culture temperature. Intracellular levels of acetyl-CoA, citrate, 2-oxoglutarate, succinate, and malate, and extracellular levels of 2-oxoglutarate, succinate, and malate, increased during l-lactate utilization, suggesting that the metabolic flux from l-lactate was distributed toward the tricarboxylic acid cycle. This study provides a perspective on l-lactate treatment by photosynthetic microorganisms, which would increase the feasibility of animal cell culture industries.
    Springer Science and Business Media LLC, May 2023, Scientific Reports, 13(1) (1), English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Erina Yoshida, Yuichi Kato, Akihiko Kanamoto, Akihiko Kondo, Tomohisa Hasunuma
    Elsevier BV, May 2023, Algal Research, 72, 103144 - 103144, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Takahiro Yukawa, Takahiro Bamba, Mami Matsuda, Takanobu Yoshida, Kentaro Inokuma, Jungyeon Kim, Jae Won Lee, Yong‐Su Jin, Akihiko Kondo, Tomohisa Hasunuma
    Wiley, Feb. 2023, Biotechnology and Bioengineering, 120(2) (2), 511 - 523, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Kentaro Inokuma, Shunya Miyamoto, Kohei Morinaga, Yuma Kobayashi, Ryota Kumokita, Takahiro Bamba, Yoichiro Ito, Akihiko Kondo, Tomohisa Hasunuma
    Wiley, Jan. 2023, Biotechnology and Bioengineering, 120(4) (4), 1097 - 1107, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Daniel A Alfaro-Sayes, Jerome Amoah, Nova Rachmadona, Shinji Hama, Tomohisa Hasunuma, Akihiko Kondo, Chiaki Ogino
    Abstract The bottleneck for the production of biofuels from microalgae consists on costly harvesting processes and low lipid production, immobilization technology could play a part on making the production of biofuels more feasible. The aim of this study was to evaluate the effect of alginate immobilization on the growth and lipid productivity of the microalgae Chlorella sorokiniana, so far, the main focus of immobilization technology has been its use for wastewater treatment and nutrient removal from effluents. The microalgae Chlorella sorokiniana was cultured in both free and immobilized forms under optimal autotrophic growth conditions. Microalgae were immobilized in calcium alginate beads generated by mixing algal cells with a sodium alginate solution, followed by extrusion into a CaCl2 solution. The results obtained in this study showed that the growth of the microalgae immobilized in alginate beads, was enhanced and achieved a dry cell weight 1.4-fold higher than that of a free cell culture, a higher light transmittance was also achieved in the alginate immobilized culture, and the lipid productivity was increased from 54.21 ± 2.48 mg l−1 d in the free cell culture to 82.22 ± 8.48 mg l−1 d in the immobilized culture. These results demonstrate the effectiveness of immobilization technology for promoting growth and lipid productivity in the microalgae Chlorella sorokiniana.
    IOP Publishing, Jan. 2023, Journal of Physics: Energy, 5(1) (1), 014019 - 014019, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Musashi Takekana, Takanobu Yoshida, Erika Yoshida, Sumika Ono, Shinnosuke Horie, Christopher J. Vavricka, Moe Hiratani, Kenji Tsuge, Jun Ishii, Yoshihiro Hayakawa, Akihiko Kondo, Tomohisa Hasunuma
    Elsevier BV, Jan. 2023, Journal of Chromatography B, 1215, 123588 - 123588, English
    [Refereed]
    Scientific journal

  • Shangjie Zhang, Feng Guo, Qiao Yang, Yujia Jiang, Shihui Yang, Jiangfeng Ma, Fengxue Xin, Tomohisa Hasunuma, Akihiko Kondo, Wenming Zhang, Min Jiang
    Synthetic methylotrophic Yarrowia lipolytica was constructed to convert methanol into biomass components and succinic acid.
    Royal Society of Chemistry (RSC), 2023, Green Chemistry, 25(1) (1), 183 - 195, English
    [Refereed]
    Scientific journal

  • Kenya Tanaka, Tomokazu Shirai, Christopher J Vavricka, Mami Matsuda, Akihiko Kondo, Tomohisa Hasunuma
    Abstract Photosynthesis must maintain stability and robustness throughout fluctuating natural environments. In cyanobacteria, dark-to-light transition leads to drastic metabolic changes from dark respiratory metabolism to CO2 fixation through the Calvin–Benson–Bassham (CBB) cycle using energy and redox equivalents provided by photosynthetic electron transfer. Previous studies have shown that catabolic metabolism supports the smooth transition into CBB cycle metabolism. However, metabolic mechanisms for robust initiation of photosynthesis are poorly understood due to lack of dynamic metabolic characterizations of dark-to-light transitions. Here, we show rapid dynamic changes (on a time scale of seconds) in absolute metabolite concentrations and 13C tracer incorporation after strong or weak light irradiation in the cyanobacterium Synechocystis sp. PCC 6803. Integration of this data enabled estimation of time-resolved nonstationary metabolic flux underlying CBB cycle activation. This dynamic metabolic analysis indicated that downstream glycolytic intermediates, including phosphoglycerate and phosphoenolpyruvate, accumulate under dark conditions as major substrates for initial CO2 fixation. Compared with wild-type Synechocystis, significant decreases in the initial oxygen evolution rate were observed in 12 h dark preincubated mutants deficient in glycogen degradation or oxidative pentose phosphate pathways. Accordingly, the degree of decrease in the initial oxygen evolution rate was proportional to the accumulated pool size of glycolytic intermediates. These observations indicate that the accumulation of glycolytic intermediates is essential for efficient metabolism switching under fluctuating light environments.
    Oxford University Press (OUP), Dec. 2022, Plant Physiology, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Ryota Hidese, Mami Matsuda, Mamiko Kajikawa, Takashi Osanai, Akihiko Kondo, Tomohisa Hasunuma
    The four-carbon (C4) dicarboxylic acids, fumarate, malate, and succinate, are the most valuable targets that must be exploited for CO2-based chemical production in the move to a sustainable low-carbon future. Cyanobacteria excrete high amounts of C4 dicarboxylic acids through glycogen fermentation in a dark anoxic environment. The enhancement of metabolic flux in the reductive TCA branch in the Cyanobacterium Synechocystis sp. PCC6803 is a key issue in the C4 dicarboxylic acid production. To improve metabolic flux through the anaplerotic pathway, we have created the recombinant strain PCCK, which expresses foreign ATP-forming phosphoenolpyruvate carboxykinase (PEPck) concurrent with intrinsic phosphoenolpyruvate carboxylase (Ppc) overexpression. Expression of PEPck concurrent with Ppc led to an increase in C4 dicarboxylic acids by autofermentation. Metabolome analysis revealed that PEPck contributed to an increase in carbon flux from hexose and pentose phosphates into the TCA reductive branch. To enhance the metabolic flux in the reductive TCA branch, we examined the effect of corn-steep liquor (CSL) as a nutritional supplement on C4 dicarboxylic acid production. Surprisingly, the addition of sterilized CSL enhanced the malate production in the PCCK strain. Thereafter, the malate and fumarate excreted by the PCCK strain are converted into succinate by the CSL-settling microorganisms. Finally, high-density cultivation of cells lacking the acetate kinase gene showed the highest production of malate and fumarate (3.2 and 2.4 g/L with sterilized CSL) and succinate (5.7 g/L with non-sterile CSL) after 72 h cultivation. The present microbial community engineering is useful for succinate production by one-pot fermentation under dark anoxic conditions.
    American Chemical Society (ACS), Dec. 2022, ACS Synthetic Biology, 11(12) (12), 4054 - 4064, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Daniel A. Alfaro-Sayes, Jerome Amoah, Shimpei Aikawa, Mami Matsuda, Tomohisa Hasunuma, Akihiko Kondo, Chiaki Ogino
    Elsevier BV, Dec. 2022, Biochemical Engineering Journal, 188, 108681 - 108681, English
    [Refereed]
    Scientific journal

  • Christopher J. Vavricka, Shunsuke Takahashi, Naoki Watanabe, Musashi Takenaka, Mami Matsuda, Takanobu Yoshida, Ryo Suzuki, Hiromasa Kiyota, Jianyong Li, Hiromichi Minami, Jun Ishii, Kenji Tsuge, Michihiro Araki, Akihiko Kondo, Tomohisa Hasunuma
    Abstract Engineering the microbial production of secondary metabolites is limited by the known reactions of correctly annotated enzymes. Therefore, the machine learning discovery of specialized enzymes offers great potential to expand the range of biosynthesis pathways. Benzylisoquinoline alkaloid production is a model example of metabolic engineering with potential to revolutionize the paradigm of sustainable biomanufacturing. Existing bacterial studies utilize a norlaudanosoline pathway, whereas plants contain a more stable norcoclaurine pathway, which is exploited in yeast. However, committed aromatic precursors are still produced using microbial enzymes that remain elusive in plants, and additional downstream missing links remain hidden within highly duplicated plant gene families. In the current study, machine learning is applied to predict and select plant missing link enzymes from homologous candidate sequences. Metabolomics-based characterization of the selected sequences reveals potential aromatic acetaldehyde synthases and phenylpyruvate decarboxylases in reconstructed plant gene-only benzylisoquinoline alkaloid pathways from tyrosine. Synergistic application of the aryl acetaldehyde producing enzymes results in enhanced benzylisoquinoline alkaloid production through hybrid norcoclaurine and norlaudanosoline pathways.
    Springer Science and Business Media LLC, Dec. 2022, Nature Communications, 13(1) (1), 1405, English
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Tomoki Oyama, Yuichi Kato, Ryota Hidese, Mami Matsuda, Minenosuke Matsutani, Satoru Watanabe, Akihiko Kondo, Tomohisa Hasunuma
    Abstract Background Microalgal lipid production has attracted global attention in next-generation biofuel research. Nitrogen starvation, which drastically suppresses cell growth, is a common and strong trigger for lipid accumulation in microalgae. We previously developed a mutant Chlamydomonas sp. KAC1801, which can accumulate lipids irrespective of the presence or absence of nitrates. This study aimed to develop a feasible strategy for stable and continuous lipid production through semi-continuous culture of KAC1801. Results KAC1801 continuously accumulated > 20% lipid throughout the subculture (five generations) when inoculated with a dry cell weight of 0.8–0.9 g L−1 and cultured in a medium containing 18.7 mM nitrate, whereas the parent strain KOR1 accumulated only 9% lipid. Under these conditions, KAC1801 continuously produced biomass and consumed nitrates. Lipid productivity of 116.9 mg L−1 day−1 was achieved by semi-continuous cultivation of KAC1801, which was 2.3-fold higher than that of KOR1 (50.5 mg L−1 day−1). Metabolome and transcriptome analyses revealed a depression in photosynthesis and activation of nitrogen assimilation in KAC1801, which are the typical phenotypes of microalgae under nitrogen starvation. Conclusions By optimizing nitrate supply and cell density, a one-step cultivation system for Chlamydomonas sp. KAC1801 under nitrate-replete conditions was successfully developed. KAC1801 achieved a lipid productivity comparable to previously reported levels under nitrogen-limiting conditions. In the culture system of this study, metabolome and transcriptome analyses revealed a nitrogen starvation-like response in KAC1801.
    Springer Science and Business Media LLC, Sep. 2022, Biotechnology for Biofuels and Bioproducts, 15(1) (1), English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Bronwyn Jean Chetty, Kentaro Inokuma, Tomohisa Hasunuma, Willem Heber van Zyl, Riaan den Haan
    Springer Science and Business Media LLC, Sep. 2022, Applied Microbiology and Biotechnology, 106(18) (18), 6347 - 6361, English
    [Refereed]
    Scientific journal

  • Yoichiro Ito, Misa Ishigami, Noriko Hashiba, Yasuyuki Nakamura, Goro Terai, Tomohisa Hasunuma, Jun Ishii, Akihiko Kondo
    Wiley, Sep. 2022, Microbial Biotechnology, 15(9) (9), 2364 - 2378, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Jyumpei Kobayashi, Daisuke Sasaki, Kiyotaka Y Hara, Tomohisa Hasunuma, Akihiko Kondo
    BACKGROUND: Glutathione is a valuable tri-peptide that is industrially produced by fermentation using the yeast Saccharomyces cerevisiae, and is widely used in the pharmaceutical, food, and cosmetic industries. It has been reported that addition of L-serine (L-Ser) is effective at increasing the intracellular glutathione content because L-Ser is the common precursor of L-cysteine (L-Cys) and glycine (Gly) which are substrates for glutathione biosynthesis. Therefore, we tried to enhance the L-Ser biosynthetic pathway in S. cerevisiae for improved glutathione production. RESULTS: The volumetric glutathione production of recombinant strains individually overexpressing SER2, SER1, SER3, and SER33 involved in L-Ser biosynthesis at 48 h cultivation was increased 1.3, 1.4, 1.9, and 1.9-fold, respectively, compared with that of the host GCI strain, which overexpresses genes involved in glutathione biosynthesis. We further examined simultaneous overexpression of SHM2 and/or CYS4 genes involved in Gly and L-Cys biosynthesis, respectively, using recombinant GCI strain overexpressing SER3 and SER33 as hosts. As a result, GCI overexpressing SER3, SHM2, and CYS4 showed the highest volumetric glutathione production (64.0 ± 4.9 mg/L) at 48 h cultivation, and this value is about 2.5-fold higher than that of the control strain. CONCLUSIONS: This study first revealed that engineering of L-Ser and Gly biosynthetic pathway are useful strategies for fermentative glutathione production by S. cerevisiase.
    Springer Science and Business Media LLC, Aug. 2022, Microbial cell factories, 21(1) (1), 153 - 153, English, International magazine
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Ryota Kumokita, Takahiro Bamba, Kentaro Inokuma, Takanobu Yoshida, Yoichiro Ito, Akihiko Kondo, Tomohisa Hasunuma
    American Chemical Society (ACS), Jun. 2022, ACS Synthetic Biology, 11(6) (6), 2098 - 2107, English
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Yoichiro Ito, Misa Ishigami, Goro Terai, Yasuyuki Nakamura, Noriko Hashiba, Teruyuki Nishi, Hikaru Nakazawa, Tomohisa Hasunuma, Kiyoshi Asai, Mitsuo Umetsu, Jun Ishii, Akihiko Kondo
    Abstract Expression of secreted recombinant proteins burdens the protein secretion machinery, limiting production. Here, we describe an approach to improving protein production by the non-conventional yeast Komagataella phaffii comprised of genome-wide screening for effective gene disruptions, combining them in a single strain, and recovering growth reduction by adaptive evolution. For the screen, we designed a multiwell-formatted, streamlined workflow to high-throughput assay of secretion of a single-chain small antibody, which is cumbersome to detect but serves as a good model of proteins that are difficult to secrete. Using the consolidated screening system, we evaluated >19,000 mutant strains from a mutant library prepared by a modified random gene-disruption method, and identified six factors for which disruption led to increased antibody production. We then combined the disruptions, up to quadruple gene knockouts, which appeared to contribute independently, in a single strain and observed an additive effect. Target protein and promoter were basically interchangeable for the effects of knockout genes screened. We finally used adaptive evolution to recover reduced cell growth by multiple gene knockouts and examine the possibility for further enhancing protein secretion. Our successful, three-part approach holds promise as a method for improving protein production by non-conventional microorganisms.
    Springer Science and Business Media LLC, Jun. 2022, Communications Biology, 5(1) (1), 561, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Atomu Yamaguchi, Noriaki Maeshige, Xiaoqi Ma, Mikiko Uemura, Hikari Noguchi, Mami Matsuda, Yuya Nishimura, Tomohisa Hasunuma, Hiroyo Kondo, Hidemi Fujino
    Informa UK Limited, Apr. 2022, Journal of Inflammation Research, Volume 15, 2387 - 2395, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Takahiro Bamba, Gregory Guirimand, Akihiko Kondo, Tomohisa Hasunuma
    Elsevier BV, Feb. 2022, Current Opinion in Green and Sustainable Chemistry, 33, 100584 - 100584, English
    Scientific journal

  • Yonghao Li, Peng Zhang, Deying Zhu, Bo Yao, Tomohisa Hasunuma, Akihiko Kondo, Xinqing Zhao
    Elsevier BV, Jan. 2022, Biochemical Engineering Journal, 178, 108296 - 108296
    Scientific journal

  • Hong-Qi Chen, Ming-Ming Zhang, Qi Xing, Pei-Liang Ye, Tomohisa Hasunuma, Akihiko Kondo, Xin-Qing Zhao
    Elsevier BV, Jan. 2022, Biochemical Engineering Journal, 178, 108274 - 108274
    Scientific journal

  • Yuma Kobayashi, Kentaro Inokuma, Mami Matsuda, Akihiko Kondo, Tomohisa Hasunuma
    Elsevier BV, Jan. 2022, Biotechnology Notes, 3, 1 - 7, English
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Yuichi Kato, Kosuke Inabe, Ryota Hidese, Akihiko Kondo, Tomohisa Hasunuma
    Elsevier BV, Jan. 2022, Bioresource Technology, 344, 126196 - 126196, English
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Ku Syahidah Ku Ismail, Yuki Matano, Yuri Sakihama, Kentaro Inokuma, Yumiko Nambu, Tomohisa Hasunuma, Akihiko Kondo
    Elsevier BV, Jan. 2022, Bioresource Technology, 343, 126071 - 126071, English
    Scientific journal

  • Kosuke Inabe, Ayaka Miichi, Mami Matsuda, Takanobu Yoshida, Yuichi Kato, Ryota Hidese, Akihiko Kondo, Tomohisa Hasunuma
    Nitrogen is essential for the biosynthesis of various molecules in cells, such as amino acids and nucleotides, as well as several types of lipids and sugars. Cyanobacteria can assimilate several forms of nitrogen, including nitrate, ammonium, and urea, and the physiological and genetic responses to these nitrogen sources have been studied previously. However, the metabolic changes in cyanobacteria caused by different nitrogen sources have not yet been characterized. This study aimed to elucidate the influence of nitrate and ammonium on the metabolic profiles of the cyanobacterium Synechocystis sp. strain PCC 6803. When supplemented with NaNO3 or NH4Cl as the nitrogen source, Synechocystis sp. PCC 6803 grew faster in NH4Cl medium than in NaNO3 medium. Metabolome analysis indicated that some metabolites in the CBB cycle, glycolysis, and TCA cycle, and amino acids were more abundant when grown in NH4Cl medium than NaNO3 medium. 15N turnover rate analysis revealed that the nitrogen assimilation rate in NH4Cl medium was higher than in NaNO3 medium. These results indicate that the mechanism of nitrogen assimilation in the GS-GOGAT cycle differs between NaNO3 and NH4Cl. We conclude that the amounts and biosynthetic rate of cyanobacterial metabolites varies depending on the type of nitrogen.
    MDPI AG, Dec. 2021, Metabolites, 11(12) (12), 867 - 867
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Hideo Kawaguchi, Tomohisa Hasunuma, Yasuo Ohnishi, Takashi Sazuka, Akihiko Kondo, Chiaki Ogino
    Abstract Background Bio-based aromatic compounds are of great interest to the industry, as commercial production of aromatic compounds depends exclusively on the unsustainable use of fossil resources or extraction from plant resources. γ-amino acid 3-amino-4-hydroxybenzoic acid (3,4-AHBA) serves as a precursor for thermostable bioplastics. Results Under aerobic conditions, a recombinant Corynebacterium glutamicum strain KT01 expressing griH and griI genes derived from Streptomyces griseus produced 3,4-AHBA with large amounts of amino acids as by-products. The specific productivity of 3,4-AHBA increased with decreasing levels of dissolved oxygen (DO) and was eightfold higher under oxygen limitation (DO = 0 ppm) than under aerobic conditions (DO ≥ 2.6 ppm). Metabolic profiles during 3,4-AHBA production were compared at three different DO levels (0, 2.6, and 5.3 ppm) using the DO-stat method. Results of the metabolome analysis revealed metabolic shifts in both the central metabolic pathway and amino acid metabolism at a DO of < 33% saturated oxygen. Based on this metabolome analysis, metabolic pathways were rationally designed for oxygen limitation. An ldh deletion mutant, with the loss of lactate dehydrogenase, exhibited 3.7-fold higher specific productivity of 3,4-AHBA at DO = 0 ppm as compared to the parent strain KT01 and produced 5.6 g/L 3,4-AHBA in a glucose fed-batch culture. Conclusions Our results revealed changes in the metabolic state in response to DO concentration and provided insights into oxygen supply during fermentation and the rational design of metabolic pathways for improved production of related amino acids and their derivatives. Graphical Abstract
    Springer Science and Business Media LLC, Dec. 2021, Microbial Cell Factories, 20(1) (1), 228, English
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Tomoki Oyama, Yuichi Kato, Katsuya Satoh, Yutaka Oono, Mami Matsuda, Tomohisa Hasunuma, Akihiko Kondo
    Elsevier BV, Dec. 2021, Algal Research, 60, 102544 - 102544
    Scientific journal

  • Yuma Kobayashi, Kentaro Inokuma, Mami Matsuda, Akihiko Kondo, Tomohisa Hasunuma
    Elsevier BV, Dec. 2021, Metabolic Engineering Communications, 13, e00188 - e00188, English
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Nozomu Shibata, Hiroshi Kakeshita, Kazuaki Igarashi, Yasushi Takimura, Yosuke Shida, Wataru Ogasawara, Tohru Koda, Tomohisa Hasunuma, Akihiko Kondo
    Abstract Background Trichoderma reesei is a filamentous fungus that is important as an industrial producer of cellulases and hemicellulases due to its high secretion of these enzymes and outstanding performance in industrial fermenters. However, the reduction of enzyme production caused by carbon catabolite repression (CCR) has long been a problem. Disruption of a typical transcriptional regulator, Cre1, does not sufficiently suppress this reduction in the presence of glucose. Results We found that deletion of an α-tubulin (tubB) in T. reesei enhanced both the amount and rate of secretory protein production. Also, the tubulin-disrupted (ΔtubB) strain had high enzyme production and the same enzyme profile even if the strain was cultured in a glucose-containing medium. From transcriptome analysis, the ΔtubB strain exhibited upregulation of both cellulase and hemicellulase genes including some that were not originally induced by cellulose. Moreover, cellobiose transporter genes and the other sugar transporter genes were highly upregulated, and simultaneous uptake of glucose and cellobiose was also observed in the ΔtubB strain. These results suggested that the ΔtubB strain was released from CCR. Conclusion Trichoderma reesei α-tubulin is involved in the transcription of cellulase and hemicellulase genes, as well as in CCR. This is the first report of overcoming CCR by disrupting α-tubulin gene in T. reesei. The disruption of α-tubulin is a promising approach for creating next-generation enzyme-producing strains of T. reesei.
    Springer Science and Business Media LLC, Dec. 2021, Biotechnology for Biofuels, 14(1) (1), 39 - 39, English, International magazine
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Yuichi Kato, Tomoki Oyama, Kentaro Inokuma, Christopher J. Vavricka, Mami Matsuda, Ryota Hidese, Katsuya Satoh, Yutaka Oono, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo
    AbstractLight/dark cycling is an inherent condition of outdoor microalgae cultivation, but is often unfavorable for lipid accumulation. This study aims to identify promising targets for metabolic engineering of improved lipid accumulation under outdoor conditions. Consequently, the lipid-rich mutant Chlamydomonas sp. KOR1 was developed through light/dark-conditioned screening. During dark periods with depressed CO2 fixation, KOR1 shows rapid carbohydrate degradation together with increased lipid and carotenoid contents. KOR1 was subsequently characterized with extensive mutation of the ISA1 gene encoding a starch debranching enzyme (DBE). Dynamic time-course profiling and metabolomics reveal dramatic changes in KOR1 metabolism throughout light/dark cycles. During light periods, increased flux from CO2 through glycolytic intermediates is directly observed to accompany enhanced formation of small starch-like particles, which are then efficiently repartitioned in the next dark cycle. This study demonstrates that disruption of DBE can improve biofuel production under light/dark conditions, through accelerated carbohydrate repartitioning into lipid and carotenoid.
    Springer Science and Business Media LLC, Dec. 2021, Communications Biology, 4(1) (1)
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Chaofan Zhang, Tomohisa Hasunuma, Su Shiung Lam, Akihiko Kondo, Shih-Hsin Ho
    Elsevier BV, Nov. 2021, Bioresource Technology, 340, 125638 - 125638, English
    Scientific journal

  • Joana T. Cunha, Daniel G. Gomes, Aloia Romaní, Kentaro Inokuma, Tomohisa Hasunuma, Akihiko Kondo, Lucília Domingues
    Elsevier BV, Sep. 2021, Energy Conversion and Management, 243, 114359 - 114359, English
    Scientific journal

  • Kentaro Inokuma, Yuki Kitada, Takahiro Bamba, Yuma Kobayashi, Takahiro Yukawa, Riaan den Haan, Willem Heber van Zyl, Akihiko Kondo, Tomohisa Hasunuma
    Springer Science and Business Media LLC, Aug. 2021, Applied Microbiology and Biotechnology, 105(14-15) (14-15), 5895 - 5904, English
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Jih-Heng Chen, Yuichi Kato, Mami Matsuda, Chun-Yen Chen, Dillirani Nagarajan, Tomohisa Hasunuma, Akihiko Kondo, Jo-Shu Chang
    Elsevier BV, Aug. 2021, Bioresource Technology, 334, 125200 - 125200, English
    Scientific journal

  • Muzhen Xu, Jeffrey Harmon, Dan Yuan, Sheng Yan, Cheng Lei, Kotaro Hiramatsu, Yuqi Zhou, Mun Hong Loo, Tomohisa Hasunuma, Akihiro Isozaki, Keisuke Goda
    In the past few decades, microalgae-based bioremediation methods for treating heavy metal (HM)-polluted wastewater have attracted much attention by virtue of their environment friendliness, cost efficiency, and sustainability. However, their HM removal efficiency is far from practical use. Directed evolution is expected to be effective for developing microalgae with a much higher HM removal efficiency, but there is no non-invasive or label-free indicator to identify them. Here, we present an intelligent cellular morphological indicator for identifying the HM removal efficiency of Euglena gracilis in a non-invasive and label-free manner. Specifically, we show a strong monotonic correlation (Spearman's ρ = -0.82, P = 2.1 × 10-5) between a morphological meta-feature recognized via our machine learning algorithms and the Cu2+ removal efficiency of 19 E. gracilis clones. Our findings firmly suggest that the morphology of E. gracilis cells can serve as an effective HM removal efficiency indicator and hence have great potential, when combined with a high-throughput image-activated cell sorter, for directed-evolution-based development of E. gracilis with an extremely high HM removal efficiency for practical wastewater treatment worldwide.
    American Chemical Society (ACS), Jun. 2021, Environmental Science & Technology, 55(12) (12), 7880 - 7889, English, International magazine
    Scientific journal

  • Shota Isogai, Nobuyuki Okahashi, Ririka Asama, Tomomi Nakamura, Tomohisa Hasunuma, Fumio Matsuda, Jun Ishii, Akihiko Kondo
    Reconstitution of prenylflavonoids using the flavonoid biosynthetic pathway and prenyltransferases (PTs) in microbes can be a promising attractive alternative to plant-based production or chemical synthesis. Here, we demonstrate that promiscuous microbial PTs can be a substitute for regiospecific but mostly unidentified botanical PTs. To test the prenylations of naringenin, we constructed a yeast strain capable of producing naringenin from l-phenylalanine by genomic integration of six exogenous genes encoding components of the naringenin biosynthetic pathway. Using this platform strain, various microbial PTs were tested for prenylnaringenin production. In vitro screening demonstrated that the fungal AnaPT (a member of the tryptophan dimethylallyltransferase family) specifically catalyzed C-3' prenylation of naringenin, whereas SfN8DT-1, a botanical PT, specifically catalyzed C-8 prenylation. In vivo, the naringenin-producing strain expressing the microbial AnaPT exhibited heterologous microbial production of 3'-prenylnaringenin (3'-PN), in contrast to the previously reported in vivo production of 8-prenylnaringenin (8-PN) using the botanical SfN8DT-1. These findings provide strategies towards expanding the production of a variety of prenylated compounds, including well-known prenylnaringenins and novel prenylflavonoids. These results also suggest the opportunity for substituting botanical PTs, both known and unidentified, that display relatively strict regiospecificity of the prenyl group transfer.
    Elsevier BV, Jun. 2021, Metabolic Engineering Communications, 12, e00169 - e00169, English, International magazine
    Scientific journal

  • Gregory Guirimand, Natalja Kulagina, Nicolas Papon, Tomohisa Hasunuma, Vincent Courdavault
    Elsevier BV, May 2021, Trends in Biotechnology, 39(5) (5), 488 - 504, English
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Saori Ogawa, Hitomi Shimidzu, Koji Fukuda, Naoki Tsunekawa, Toshiyuki Hirano, Fumitoshi Sato, Kei Yura, Tomohisa Hasunuma, Kozo Ochi, Michio Yamamoto, Wataru Sakamoto, Kentaro Hashimoto, Hiroyuki Ogata, Tadayoshi Kanao, Michiko Nemoto, Kenji Inagaki, Takashi Tamura
    ABSTRACT Streptomyces incarnatus NRRL8089 produces the antiviral, antifungal, antiprotozoal nucleoside antibiotic sinefungin. To enhance sinefungin production, multiple mutations were introduced to the rpoB gene encoding RNA polymerase (RNAP) β-subunit at the target residues, D447, S453, H457, and R460. Sparse regression analysis using elastic-net lasso-ridge penalties on previously reported H457X mutations identified a numeric parameter set, which suggested that H457R/Y/F may cause production enhancement. H457R/R460C mutation successfully enhanced the sinefungin production by 3-fold, while other groups of mutations, such as D447G/R460C or D447G/H457Y, made moderate or even negative effects. To identify why the rif cluster residues have diverse effects on sinefungin production, an RNAP/DNA/mRNA complex model was constructed by homology modeling and molecular dynamics simulation. The 4 residues were located near the mRNA strand. Density functional theory–based calculation suggested that D447, H457, and R460 are in direct contact with ribonucleotide, and partially positive charges are induced by negatively charged chain of mRNA.
    Oxford University Press (OUP), Apr. 2021, Bioscience, Biotechnology, and Biochemistry, 85(5) (5), 1275 - 1282, English
    Scientific journal

  • Takahiro Yukawa, Takahiro Bamba, Gregory Guirimand, Mami Matsuda, Tomohisa Hasunuma, Akihiko Kondo
    Wiley, Jan. 2021, Biotechnology and Bioengineering, 118(1) (1), 175 - 185, English
    Scientific journal

  • Musashi Takenaka, Takanobu Yoshida, Yoshimi Hori, Takahiro Bamba, Masao Mochizuki, Christopher J. Vavricka, Takanari Hattori, Yoshihiro Hayakawa, Tomohisa Hasunuma, Akihiko Kondo
    Elsevier BV, Jan. 2021, Talanta, 222, 121625 - 121625, English
    Scientific journal

  • Yoichiro Ito, Goro Terai, Misa Ishigami, Noriko Hashiba, Yasuyuki Nakamura, Takahiro Bamba, Ryota Kumokita, Tomohisa Hasunuma, Kiyoshi Asai, Jun Ishii, Akihiko Kondo
    Abstract In the yeast Saccharomyces cerevisiae, terminator sequences not only terminate transcription but also affect expression levels of the protein-encoded upstream of the terminator. The non-conventional yeast Pichia pastoris (syn. Komagataella phaffii) has frequently been used as a platform for metabolic engineering but knowledge regarding P. pastoris terminators is limited. To explore terminator sequences available to tune protein expression levels in P. pastoris, we created a ‘terminator catalog’ by testing 72 sequences, including terminators from S. cerevisiae or P. pastoris and synthetic terminators. Altogether, we found that the terminators have a tunable range of 17-fold. We also found that S. cerevisiae terminator sequences maintain function when transferred to P. pastoris. Successful tuning of protein expression levels was shown not only for the reporter gene used to define the catalog but also using betaxanthin production as an example application in pathway flux regulation. Moreover, we found experimental evidence that protein expression levels result from mRNA abundance and in silico evidence that levels reflect the stability of mRNA 3′-UTR secondary structure. In combination with promoter selection, the novel terminator catalog constitutes a basic toolbox for tuning protein expression levels in metabolic engineering and synthetic biology in P. pastoris.
    Oxford University Press (OUP), Dec. 2020, Nucleic Acids Research, 48(22) (22), 13000 - 13012, English
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Japan Computational Mass Spectrometry Meeting 2020 Activity Report
    Hiroyuki Yamamoto, Eisuke Hayakawa, Hiroshi Tsugawa, Yuki Moriya, Eiichiro Fukusaki, Susumu Goto, Tomohisa Hasunuma, Nobuaki Miura, Akiyasu C. Yoshizawa
    Dec. 2020, Journal of Proteome Data and Methods, 2(0005) (0005), English
    Scientific journal

  • Nao Nitta, Takanori Iino, Akihiro Isozaki, Mai Yamagishi, Yasutaka Kitahama, Shinya Sakuma, Yuta Suzuki, Hiroshi Tezuka, Minoru Oikawa, Fumihito Arai, Takuya Asai, Dinghuan Deng, Hideya Fukuzawa, Misa Hase, Tomohisa Hasunuma, Takeshi Hayakawa, Kei Hiraki, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Yuki Inoue, Takuro Ito, Masataka Kajikawa, Hiroshi Karakawa, Yusuke Kasai, Yuichi Kato, Hirofumi Kobayashi, Cheng Lei, Satoshi Matsusaka, Hideharu Mikami, Atsuhiro Nakagawa, Keiji Numata, Tadataka Ota, Takeichiro Sekiya, Kiyotaka Shiba, Yoshitaka Shirasaki, Nobutake Suzuki, Shunji Tanaka, Shunnosuke Ueno, Hiroshi Watarai, Takashi Yamano, Masayuki Yazawa, Yusuke Yonamine, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Keisuke Goda
    Springer Science and Business Media LLC, Dec. 2020, Nature Communications, 11(1) (1), 3452, English
    [Refereed]
    Scientific journal

  • Joana T. Cunha, Aloia Romaní, Kentaro Inokuma, Björn Johansson, Tomohisa Hasunuma, Akihiko Kondo, Lucília Domingues
    AbstractConsolidated bioprocessing, which combines saccharolytic and fermentative abilities in a single microorganism, is receiving increased attention to decrease environmental and economic costs in lignocellulosic biorefineries. Nevertheless, the economic viability of lignocellulosic ethanol is also dependent of an efficient utilization of the hemicellulosic fraction, which is mainly composed of xylose and may comprise up to 40 % of the total biomass. This major bottleneck is mainly due to the necessity of chemical/enzymatic treatments to hydrolyze hemicellulose into fermentable sugars and to the fact that xylose is not readily consumed by Saccharomyces cerevisiae – the most used organism for large-scale ethanol production. In this work, industrial S. cerevisiae strains, presenting robust traits such as thermotolerance and improved resistance to inhibitors, were evaluated as hosts for the cell-surface display of hemicellulolytic enzymes and optimized xylose assimilation, aiming at the development of whole-cell biocatalysts for consolidated bioprocessing of corn cob-derived hemicellulose. These modifications allowed the direct production of ethanol from non-detoxified hemicellulosic liquor obtained by hydrothermal pretreatment of corn cob, reaching an ethanol titer of 11.1 g/L corresponding to a yield of 0.328 gram per gram of potential xylose and glucose, without the need for external hydrolytic catalysts. Also, consolidated bioprocessing of pretreated corn cob was found to be more efficient for hemicellulosic ethanol production than simultaneous saccharification and fermentation with addition of commercial hemicellulases. These results show the potential of industrial S. cerevisiae strains for the design of whole-cell biocatalysts and paves the way for the development of more efficient consolidated bioprocesses for lignocellulosic biomass valorization, further decreasing environmental and economic costs.
    Cold Spring Harbor Laboratory, Jul. 2020, Biotechnology for Biofuels, 13(1) (1), 1 - 15, English
    [Refereed]
    Scientific journal

  • Wenying Qu, Pau Loke Show, Tomohisa Hasunuma, Shih-Hsin Ho
    Elsevier BV, Jun. 2020, Bioresource Technology, 305, 123072 - 123072, English
    [Refereed]
    Scientific journal

  • Sequentially addressable dielectrophoretic array for high-throughput sorting of large-volume biological compartments
    1. A. Isozaki, Y. Nakagawa, M. H. Loo, Y. Shibata, N. Tanaka, D. L. Setyaningrum, J.-W. Park, Y. Shirasaki, H. Mikami, D. Huang, H. Tsoi, C. T. Riche, T. Ota, H. Miwa, Y. Kanda, T. Ito, K. Yamada, O. Iwata, K. Suzuki, S. Ohnuki, Y. Ohya, Y. Kato, T. Hasunuma, S. Matsusaka, M. Yamagishi, M. Yazawa, S. Uemura, K. Nagasawa, H. Watarai, D. Di Carlo, K. Goda
    May 2020, Science Advances, 6, eaba6712, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Ryota Hidese, Mami Matsuda, Takashi Osanai, Tomohisa Hasunuma, Akihiko Kondo
    d-Lactate is one of the most valuable compounds for manufacturing biobased polymers. Here, we have investigated the significance of endogenous malate dehydrogenase (decarboxylating) (malic enzyme, ME), which catalyzes the oxidative decarboxylation of malate to pyruvate, in d-lactate biosynthesis in the cyanobacterium Synechocystis sp. PCC6803. d-Lactate levels were increased by 2-fold in ME-overexpressing strains, while levels in ME-deficient strains were almost equivalent to those in the host strain. Dynamic metabolomics revealed that overexpression of ME led to increased turnover rates in malate and pyruvate metabolism; in contrast, deletion of ME resulted in increased pool sizes of glycolytic intermediates, probably due to sequential feedback inhibition, initially triggered by malate accumulation. Finally, both the loss of the acetate kinase gene and overexpression of endogenous d-lactate dehydrogenase, concurrent with ME overexpression, resulted in the highest production of d-lactate (26.6 g/L) with an initial cell concentration of 75 g-DCW/L after 72 h fermentation.
    Feb. 2020, ACS synthetic biology, 9(2) (2), 260 - 268, English, International magazine
    [Refereed]
    Link to Kobe Univ. Repository (Kernel)

  • Novel strategy for anchorage position control of GPI-attached proteins in the yeast cell wall using different GPI-anchoring domains
    Kentaro Inokuma, Hiroki Kurono, Riaan den, Haan, Willem Heber van Zyl, Tomohisa Hasunuma, Akihiko Kondo
    Jan. 2020, Metabolic Engineering, 57, 110 - 117, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Dynamic metabolomics for engineering biology: Accelerating learning cycles for bioproduction
    Christopher J. Vavricka, Tomohisa Hasunuma, Akihiko Kondo
    Jan. 2020, Trends in Biotechnology, 38(1) (1), 68 - 82, English
    [Refereed]
    Scientific journal

  • Production of 1,2,4-butanetriol from xylose by Saccharomyces cerevisiae through Fe metabolic engineering
    Takahiro Bamba, Takahiro Yukawa, Gregory Guirimand, Kentaro Inokuma, Kengo Sasaki, Tomohisa Hasunuma, Akihiko Kondo
    Dec. 2019, Metabolic Engineering, 56, 17 - 27, English
    [Refereed]
    Scientific journal

  • Christopher J. Vavricka, Takanobu Yoshida, Yuki Kuriya, Shunsuke Takahashi, Teppei Ogawa, Fumie Ono, Kazuko Agari, Hiromasa Kiyota, Jianyong Li, Jun Ishii, Kenji Tsuge, Hiromichi Minami, Michihiro Araki, Tomohisa Hasunuma, Akihiko Kondo
    Dec. 2019, Nature Communications, 10(1) (1)
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Short-term temporal metabolic behavior in halophilic cyanobacterium Synechococcus sp. strain PCC 7002 after salt shock
    Shimpei Aikawa, Atsumi Nishida, Tomohisa Hasunuma, Jo-Shu Chang, Akihiko Kondo
    Dec. 2019, Metabolites, 9(12) (12), 297, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Fermentation of pigment-extracted microalgal residue using yeast cell-surface display: direct high-density ethanol production with competitive life cycle impacts
    Xiaochen Huang, Shunwen Bai, Zhuo Liu, Tomohisa Hasunuma, Akihiko Kondo, Shih-Hsin Ho
    Nov. 2019, Green Chemistry, 22(1) (1), 153 - 162, English
    [Refereed]
    Scientific journal

  • A novel process for the mixotrophic production of lutein with Chlorella sorokiniana MB-1-M12 using aquaculture wastewater
    Jih-Heng Chen, Yuichi Kato, Mami Matsuda, Chun-Yen Chen, Dillirani Nagarajan, Tomohisa Hasunuma, Akihiko Kondo, Cheng-Di Dong, Duu-Jong Lee, Jo-Shu Chang
    Oct. 2019, Bioresource Technology, 290, 121786, English
    [Refereed]
    Scientific journal

  • Single-Stage Astaxanthin Production Enhances the Nonmevalonate Pathway and Photosynthetic Central Metabolism in Synechococcus sp. PCC 7002
    Tomohisa Hasunuma, Ayako Takaki, Mami Matsuda, Yuichi Kato, Christopher J. Vavricka, Akihiko Kondo
    Oct. 2019, ACS Synthetic Biology, 8, 2701 - 2709, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Combined cell surface display of β-D-glucosidase (BGL), maltose transporter (MAL11) and overexpression of cytosolic xylose reductase (XR) in Saccharomyces cerevisiae enhance cellobiose/xylose co-utilization for xylitol bio-production from lignocellulosic
    Gregory G, Y. Guirimand, Takahiro Bamba, Mami Matsuda, Kentaro Inokuma, Kenta Morita, Yuki Kitada, Yuma Kobayashi, Takahiro Yukawa, Kengo Sasaki, Chiaki Ogino, Tomohisa Hasunuma, Akihiko Kondo
    Sep. 2019, Biotechnology Journal, 14(9) (9), e1800704, English
    [Refereed]
    Scientific journal

  • Day/night separation of oxygenic energy metabolism and nuclear DNA replication in the unicellular red alga Cyanidioschyzon merolae
    Shin-ya Miyagishima, Atsuko Era, Tomohisa Hasunuma, Mami Matsuda, Shunsuke Hirooka, Nobuko Sumiya, Akihiko Kondo, Takayuki Fujiwara
    Jul. 2019, mBio, 10(4) (4), e00833-19, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Cell-surface display technology and metabolic engineering of Saccharomyces cerevisiae for enhancing xylitol production from woody biomass
    Gregory Guirimand, Kentaro Inokuma, Takahiro Bamba, Mami Matsuda, Kenta Morita, Kengo Sasaki, Chiaki Ogino, Jean-Guy Berrin, Tomohisa Hasunuma, Akihiko Kondo
    May 2019, Green Chemistry, 21, 1795 - 1808, English
    [Refereed]
    Scientific journal

  • Mechanism-based tuning of insect 3,4-dihydroxyphenylacetaldehyde synthase for synthetic bioproduction of benzylisoquinoline alkaloids
    Christopher John, Vavricka Jr, Takanobu Yoshida, Yuki Kuriya, Shunsuke Takahashi, Teppei Ogawa, Fumie Ono, Kazuko Agari, Hiromasa Kiyota, Jianyong Li, Jun Ishii, Kenji Tsuge, Hiromichi Minami, Michihiro Araki, Tomohisa Hasunuma, Akihiko Kondo
    May 2019, Nature Communications, 10, 2015, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Enhancing lutein production with mixotrophic cultivation of Chlorella sorokiniana MB-1-M12 using different bioprocess operation strategies
    Jih-Heng Chen, Chun-Yen Chen, Tomohisa Hasunuma, Akihiko Kondo, Chien-Hsiang Chang, I-Son Ng, Jo-Shu Chang
    Apr. 2019, Bioresource Technology, 278, 17 - 25, English
    [Refereed]
    Scientific journal

  • SAKIHAMA Yuri, HIDESE Ryota, HASUNUMA Tomohisa, KONDO Akihiko
    Yeasts are extremely useful, not only for fermentation but also for a wide spectrum of fuel and chemical productions. We analyzed the overall metabolic turnover and transcript dynamics in glycolysis and the TCA cycle, revealing the difference in adaptive pyruvate metabolic response between a Crabtree-negative species, Kluyveromyces marxianus, and a Crabtree-positive species, Saccharomyces cerevisiae, during aerobic growth. Pyruvate metabolism was inclined toward ethanol production under aerobic conditions in S. cerevisiae, while increased transcript abundances of the genes involved in ethanol metabolism and those encoding pyruvate dehydrogenase were seen in K. marxianus, indicating the augmentation of acetyl-CoA synthesis. Furthermore, different metabolic turnover in the TCA cycle was observed in the two species: malate and fumarate production in S. cerevisiae was higher than in K. marxianus, irrespective of aeration; however, fluxes of both the reductive and oxidative TCA cycles were enhanced in K. marxianus by aeration, implying both the cycles contribute to efficient electron flux without producing ethanol. Additionally, decreased hexokinase activity under aerobic conditions is expected to be important for maintenance of suitable carbon flux. These findings demonstrate differences in the key metabolic trait of yeasts employing respiration or fermentation, and provide important insight into the metabolic engineering of yeasts.
    Mar. 2019, Scientific Reports, 9(1) (1), 5319 - 5319, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Sustainable production of glutathione from lignocellulose-derived sugars using engineered Saccharomyces cerevisiae
    KOBAYASHI Jyumpei, SASAKI Daisuke, BAMBA Takahiro, HASUNUMA Tomohisa, KONDO Akihiko
    Feb. 2019, Applied Microbiology and Biotechnology, 103(3) (3), 1243 - 1254, English
    [Refereed]
    Scientific journal

  • Light/dark cycling causes delayed lipid accumulation and increased photoperiod-based biomass yield by altering metabolic flux in oleaginous Chlamydomonas sp.
    Yuichi KATO, Yusuke FUJIHARA, Christopher J. VAVRICKA, Jo-Shu CHANG, Tomohisa HASUNUMA, Akihiko KONDO
    Feb. 2019, Biotechnology for Biofuels, 12, 39, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • 5-Hydroxymethylfurfural production from salt-induced photoautotrophically cultivated Chlorella sorokiniana
    AMOAH Jerome, HASUNUMA Tomohisa, OGINO Chiaki, KONDO Akihiko
    Feb. 2019, Biochemical Engineering Journal, 142, 117 - 123, English
    [Refereed]
    Scientific journal

  • Yoichiro Ito, Toru Watanabe, Shimpei Aikawa, Teruyuki Nishi, Tozo Nishiyama, Yasuyuki Nakamura, Tomohisa Hasunuma, Yuji Okubo, Jun Ishii, Akihiko Kondo
    Oxford University Press (OUP), Nov. 2018, FEMS Yeast Research, 18(7) (7), foy074, English
    [Refereed]
    Scientific journal

  • Widespread effect of N-acetyl-D-glucosamine assimilation on the metabolisms of amino acids, purines, and pyrimidines in Scheffersomyces stipitis
    INOKUMA Kentaro, MATSUDA Mami, SASAKI Daisuke, HASUNUMA Tomohisa, KONDO Akihiko
    Sep. 2018, Microbial Cell Factories, 17, 153, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Estimating the protein burden limit of yeast cells by measuring expression limits of glycolytic proteins
    EGUCHI Yuichi, MAKANAE Koji, HASUNUMA Tomohisa, ISHIBASHI Yuko, KITO Keiji, MORIYA Hisao
    Aug. 2018, eLife, 7, e34595, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Tomohisa Hasunuma, Mami Matsuda, Yuichi Kato, Christopher John Vavricka, Akihiko Kondo
    Academic Press Inc., Jul. 2018, Metabolic Engineering, 48, 109 - 120, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Yasuyuki Nakamura, Teruyuki Nishi, Risa Noguchi, Yoichiro Ito, Toru Watanabe, Tozo Nishiyama, Sinpei Aikawa, Tomohisa Hasunuma, Jun Ishii, Yuji Okubo, Akihiko Kondo
    ABSTRACT The methylotrophic yeast Pichia pastoris is widely used to produce recombinant proteins, taking advantage of this species' high-density cell growth and strong ability to secrete proteins. Circular plasmids containing the P. pastoris-specific autonomously replicating sequence (PARS1) permit transformation of P. pastoris with higher efficiency than obtained following chromosomal integration by linearized DNA. Unfortunately, however, existing autonomously replicating plasmids are known to be inherently unstable. In this study, we used transcriptome sequencing (RNA-seq) data and genome sequence information to independently identify, on each of the four chromosomes, centromeric DNA sequences consisting of long inverted repeat sequences. By examining the chromosome 2 centromeric DNA sequence (Cen2) in detail, we demonstrate that an ∼111-bp region located at one end of the putative centromeric sequence had autonomous replication activity. In addition, the full-length Cen2 sequence, which contains two long inverted repeat sequences and a nonrepetitive central core region, is needed for the accurate replication and distribution of plasmids in P. pastoris. Thus, we constructed a new, stable, autonomously replicating plasmid vector that harbors the entire Cen2 sequence; this episome facilitates genetic manipulation in P. pastoris, providing high transformation efficiency and plasmid stability. IMPORTANCE Secretory production of recombinant proteins is the most important application of the methylotrophic yeast Pichia pastoris, a species that permits mass production of heterologous proteins. To date, the genetic engineering of P. pastoris has relied largely on integrative vectors due to the lack of user-friendly tools. Autonomously replicating Pichia plasmids are expected to facilitate genetic manipulation; however, the existing systems, which use autonomously replicating sequences (ARSs) such as the P. pastoris-specific ARS (PARS1), are known to be inherently unstable for plasmid replication and distribution. Recently, the centromeric DNA sequences of P. pastoris were identified in back-to-back studies published by several groups; therefore, a new episomal plasmid vector with centromere DNA as a tool for genetic manipulation of P. pastoris is ready to be developed.
    American Society for Microbiology, Jul. 2018, Applied and Environmental Microbiology, 84(15) (15), e02882 - 17, English
    [Refereed]
    Scientific journal

  • Metabolome analysis-based design and engineering of a metabolic pathway in Corynebacterium glutamicum to match rates of simultaneous utilization of D-glucose and L-arabinose
    KAWAGUCHI Hideo, YOSHIHARA K, HARA KY, HASUNUMA Tomohisa, OGINO Chiaki, KONDO Akihiko
    May 2018, Microbial Cell Factories, 17, 76, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Mathematical model for small size time series data of bacterial secondary metabolic pathways
    TOMINAGA D, KAWAGUCHI Hideo, HORI Yoshimi, HASUNUMA Tomohisa, OGINO Chiaki, ABURATANI S
    May 2018, Bioinformatics and Biology Insights, 12, 1 - 7, English
    [Refereed]
    Scientific journal

  • Genetic and physiological basis for antibody production by Kluyveromyces marxianus
    NAMBU-NISHIDA Yumiko, NISHIDA Keiji, HASUNUMA Tomohisa, KONDO Akihiko
    Apr. 2018, AMB Express, 8, 56, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Enhanced cell-surface display of a heterologous protein using SED1 anchoring system in SED1-disrupted Saccharomyces cerevisiae strain
    Takahiro Bamba, Kentaro Inokuma, Tomohisa Hasunuma, Akihiko Kondo
    Mar. 2018, Journal of Bioscience and Bioengineering, 125(3) (3), 306 - 310, English
    [Refereed]
    Scientific journal

  • Direct and highly productive conversion of cyanobacteria Arthrospira platensis to ethanol with CaCl2 addition
    AIKAWA S, INOKUMA Kentaro, WAKAI Satoshi, SASAKI Kengo, OGINO Chiaki, J. S. Chang, HASUNUMA Tomohisa, KONDO Akihiko
    Feb. 2018, Biotechnology for Biofuels, 11, 50, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Yumiko Nambu-Nishida, Yuri Sakihama, Jun Ishii, Tomohisa Hasunuma, Akihiko Kondo
    Elsevier B.V., Jan. 2018, Journal of Bioscience and Bioengineering, 125(1) (1), 76 - 86, English
    [Refereed]
    Scientific journal

  • Improvement of Xylose Fermentation Ability under Heat and Acid Co-Stress in Saccharomyces cerevisiae Using Genome Shuffling Technique
    Kentaro Inokuma, Ryo Iwamoto, Takahiro Bamba, Tomohisa Hasunuma, Akihiko Kondo
    Dec. 2017, Frontiers in Bioengineering and Biotechnology, 5, 81, English
    [Refereed]
    Scientific journal

  • Yuichi Kato, Shih-Hsin Ho, Christopher J. Vavricka, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo
    Dec. 2017, BIORESOURCE TECHNOLOGY, 245(part B) (part B), 1484 - 1490, English
    [Refereed]
    Scientific journal

  • Jerome Amoah, Shih-Hsin Ho, Shinji Hama, Ayumi Yoshida, Akihito Nakanishi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo
    Dec. 2017, ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 28, 16 - 23, English
    [Refereed]
    Scientific journal

  • M. J. Mert, S. H. Rose, D. C. la Grange, T. Bamba, T. Hasunuma, A. Kondo, W. H. van Zyl
    Oct. 2017, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 44(10) (10), 1459 - 1470, English
    [Refereed]
    Scientific journal

  • Synthesis of sulfo-sialic acid analogues: potent neuraminidase inhibitors in regards to anomeric functionality
    VAVRICKA JR, JOHN, CHRISTOPHER, MUTO C, HASUNUMA Tomohisa, KIMURA Y, ARAKI Michihiro, WU Y, GAO GF, OHRUI H, IZUMI M, KIYOTA H
    Aug. 2017, Scientific Reports, 7(1) (1), 8239, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Development of a comprehensive set of tools for genome engineering in a cold- and thermo-tolerant Kluyveromyces marxianus yeast strain
    NAMBU-NISHIDA Yumiko, NISHIDA KEIJI, HASUNUMA TOMOHISA, KONDO AKIHIKO
    Aug. 2017, Scientific Reports, 7(1) (1), 8993, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Zhuo Liu, Kentaro Inokuma, Shih-Hsin Ho, Riaan den Haan, Willem H. van Zyl, Tomohisa Hasunuma, Akihiko Kondo
    Jun. 2017, BIOTECHNOLOGY AND BIOENGINEERING, 114(6) (6), 1201 - 1207, English
    [Refereed]
    Scientific journal

  • Dynamic metabolic profiling together with transcription analysis reveals salinity-induced starch-to-lipid biosynthesis in alga Chlamydomonas sp. JSC4
    HO SHIH-HSIN, NAKANISHI Akihito, KATO Yuichi, YAMASAKI H, CHANG JS, MISAWA N, HIROSE Y, MINAGAWA J, HASUNUMA Tomohisa, KONDO Akihiko
    Apr. 2017, Scientific Reports, 4(7) (7), 45471, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Simultaneous conversion of free fatty acids and triglycerides to biodiesel by immobilized Aspergillus oryzae expressing Fusarium heterosporum lipase
    AMOAH JEROME, EMMANUEL QUAYSON, HAMA S, YOSHIDA A, HASUNUMA TOMOHISA, OGINO Chiaki, KONDO Akihiko
    Mar. 2017, Biotechnology Journal, 12(3) (3), 1600400, English
    [Refereed]
    Scientific journal

  • Musashi Takenaka, Takuya Kobayashi, Kentaro Inokuma, Tomohisa Hasunuma, Tatsuo Maruyama, Chiaki Ogino, Akihiko Kondo
    Mar. 2017, COLLOIDS AND SURFACES B-BIOINTERFACES, 151, 134 - 142, English
    [Refereed]
    Scientific journal

  • Enzymatic improvement of mitochondrial thiol oxidase Erv1 for oxidized glutathione fermentation by Saccharomyces cerevisiae
    KOBAYASHI Jyumpei, SASAKI Daisuke, HARA Y Kiyotaka, HASUNUMA Tomohisa, KONDO Akihiko
    Mar. 2017, Microbial Cell Factories, 16, 44, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • A systematic approach to time-series metabolite profiling and RNA-seq analysis of Chinese hamster ovary cell culture
    HSU H, ARAKI Michihiro, MOCHIZUKI Masao, HORI Yoshimi, MURATA Masahiro, PRIHARDI Kahar, YOSHIDA Takanobu, HASUNUMA TOMOHISA, KONDO Akihiko
    Mar. 2017, Scientific Reports, 7, 43518, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Feng Zhang, Houng-Wei Wang, Keisuke Tominaga, Michitoshi Hayashi, Tomohisa Hasunuma, Akihiko Kondo
    Feb. 2017, CHEMISTRY-AN ASIAN JOURNAL, 12(3) (3), 324 - 331, English
    [Refereed]
    Scientific journal

  • Application of LC-MS/MS analysis for time-lapse amino acid metabolomics in CHO cell culture.
    Hsu H, Hasunuma T, Araki M, Yoshida T, Hori Y, Murata M, Kondo A
    Feb. 2017, Shimadzu Journal, 5(1) (1), 17 - 21, English
    [Refereed]
    Scientific journal

  • Kentaro Inokuma, Jun Ishii, Kiyotaka Y. Hara, Masao Mochizuki, Tomohisa Hasunuma, Akihiko Kondo
    American Society for Microbiology, 2017, Genome Announcements, 5(5) (5), English
    [Refereed]
    Scientific journal

  • A RuBisCO-mediated carbon metabolic pathway in methanogenic archaea
    KONO T, MEHROTRA S, ENDO C, KIZU N, MATSUDA Mami, KIMURA H, MIZOHATA E, INOUE T, HASUNUMA Tomohisa, YOKOTA A, MATSUMURA H, AHIDA H
    Jan. 2017, Nature Communications, 8, 14007, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Succinate and lactate production from Euglena gracilis during dark, anaerobic conditions.
    Tomita Y, Yoshioka K, Iijima H, Nakashima A, Iwata O, Suzuki K, Hasunuma T, Kondo A, HIRAI MY, OSANAI T
    Dec. 2016, Frontiers in Microbiology, 7, 2050, English
    [Refereed]
    Scientific journal

  • Hideo Kawaguchi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo
    Dec. 2016, CURRENT OPINION IN BIOTECHNOLOGY, 42, 30 - 39, English
    [Refereed]
    Scientific journal

  • Tomohisa Hasunuma, Akihiko Kondo
    Wiley-VCH Verlag, Nov. 2016, Industrial Biotechnology: Products and Processes, 161 - 185, English
    [Refereed]
    In book

  • Misato Ohtani, Keiko Morisaki, Yuji Sawada, Ryosuke Sano, Abigail Loren Tung Uy, Atsushi Yamamoto, Tetsuya Kurata, Yoshimi Nakano, Shiro Suzuki, Mami Matsuda, Tomohisa Hasunuma, Masami Yokota Hirai, Taku Demura
    Nov. 2016, PLANT PHYSIOLOGY, 172(3) (3), 1612 - 1624, English
    [Refereed]
    Scientific journal

  • Mamoru Ueno, Penporn Sae-Tang, Yuri Kusama, Yukako Hihara, Mami Matsuda, Tomohisa Hasunuma, Yoshitaka Nishiyama
    Nov. 2016, PLANT AND CELL PHYSIOLOGY, 57(11) (11), 2417 - 2426, English
    [Refereed]
    Scientific journal

  • High-speed scanning for the quantitative evaluation of glycogen concentration in bioethanol feedstock Synechocystis sp. PCC6803 by a near-infrared hyperspectral imaging system with a new near-infrared spectral camera.
    Ishigaki M, Nakanishi A, Hasunuma T, Kondo A, MORISHIMA T, OKUNO T, OZAKI Y
    Nov. 2016, Applied Spectroscopy, 71(3) (3), 463 - 471, English
    [Refereed]
    Scientific journal

  • Kentaro Inokuma, Takahiro Bamba, Jun Ishii, Yoichiro Ito, Tomohisa Hasunuma, Akihiko Kondo
    Nov. 2016, BIOTECHNOLOGY AND BIOENGINEERING, 113(11) (11), 2358 - 2366, English
    [Refereed]
    Scientific journal

  • Kentaro Inokuma, Tomohisa Hasunuma, Akihiko Kondo
    Oct. 2016, AMB EXPRESS, 6, 83, English
    [Refereed]
    Scientific journal

  • Zhuo Liu, Shih-Hsin Ho, Tomohisa Hasunuma, Jo-Shu Chang, Nan-Qi Ren, Akihiko Kondo
    Sep. 2016, BIORESOURCE TECHNOLOGY, 215, 324 - 333, English
    [Refereed]
    Scientific journal

  • Hiroko Iijima, Tomokazu Shirai, Mami Okamoto, Filipe Pinto, Paula Tamagnini, Tomohisa Hasunuma, Akihiko Kondo, Masami Yokota Hirai, Takashi Osanai
    Sep. 2016, ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 18, 305 - 313, English
    [Refereed]
    Scientific journal

  • Yue Wang, Sheng-Yi Chiu, Shih-Hsin Ho, Zhuo Liu, Tomohisa Hasunuma, Ting-Ting Chang, Kuan-Fu Chang, Jo-Shu Chang, Nan-Qi Ren, Akihiko Kondo
    Aug. 2016, BIOTECHNOLOGY JOURNAL, 11(8) (8), 1072 - 1081, English
    [Refereed]
    Scientific journal

  • Jerome Amoah, Shih-Hsin Ho, Shinji Hama, Ayumi Yoshida, Akihito Nakanishi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo
    Jul. 2016, BIORESOURCE TECHNOLOGY, 211, 224 - 230, English
    [Refereed]
    Scientific journal

  • Improved sugar-free succinate production by Synechocystis sp. PCC 6803 following identification of the limiting steps in glycogen catabolism.
    Hasunuma T, Matsuda M, Kondo A
    May 2016, Metabolic Engineering Communications, 3, 130 - 141, English
    [Refereed]
    Scientific journal

  • Engineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel production.
    Liu Z, Ho S.H, Sasaki K, Haan R, Inokuma K, Ogino C, van Zyl W.H, Hasunuma T, Kondo A
    Apr. 2016, Scientific Reports, 6, 24550, English
    [Refereed]
    Scientific journal

  • Gregory Guirimand, Kengo Sasaki, Kentaro Inokuma, Takahiro Bamba, Tomohisa Hasunuma, Akihiko Kondo
    Apr. 2016, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 100(8) (8), 3477 - 3487, English
    [Refereed]
    Scientific journal

  • Yota Tsuge, Motonori Kudou, Hideo Kawaguchi, Jun Ishii, Tomohisa Hasunuma, Akihiko Kondo
    Mar. 2016, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 100(6) (6), 2685 - 2692, English
    [Refereed]
    Scientific journal

  • Disruption of PHO13 improves ethanol production via the xylose isomerase pathway
    BAMBA Takahiro, HASUNUMA Tomohisa, KONDO Akihiko
    Mar. 2016, AMB Express, 6(1) (1), 4, English
    [Refereed]
    Scientific journal

  • 川口,秀夫, 寺村,浩, 中村,聡子, 荻野,千秋, 原,清敬, 蓮沼,誠久, 老沼,研一, 高谷,直樹, 平野,恒, 佐塚,隆志, 北野,英己, 近藤,昭彦
    Sorghum bagasse pretreated with diluted acid, which was predominantly composed of glucan (59%) and xylan (7.2%), was used as a lignocellulosic feedstock to produce D-phenyllactic acid (PhLA) by a recombinant Escherichia coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens. Compared to filter paper hydrolysate, the PhLA yield was reduced by 35% during fermentation with enzymatic hydrolysate of sorghum bagasse as a carbon source, and metabolomics analysis revealed that intracellular levels of erythrose-4-phosphate and phosphoenolpyruvate and NAD(P)H regeneration for PhLA production from glucose markedly reduced. Compared to the separate hydrolysis and fermentation (SHF) with sorghum bagasse hydrolysate, simultaneous saccharification and fermentation (SSF) of sorghum bagasse under glucose limitation conditions yielded 4.8-fold more PhLA with less accumulation of eluted components of p-coumaric acid and aldehydes, which inhibited PhLA fermentation. These results suggest that gradual hydrolysis of sorghum bagasse during SSF reduces the accumulation of both glucose and fermentation inhibitors, collectively leading to increased PhLA yield.
    The Japan Institute of Energy, Jan. 2016, Proceedings of the Conference on Biomass Science, 11, 35 - 36, Japanese
    [Refereed]
    International conference proceedings

  • Tomohisa Hasunuma, Takatoshi Sakamoto, Akihiko Kondo
    Jan. 2016, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 100(2) (2), 1027 - 1038, English
    [Refereed]
    Scientific journal

  • Jerome Amoah, Shih-Hsin Ho, Shinji Hama, Ayumi Yoshida, Akihito Nakanishi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo
    Jan. 2016, BIOCHEMICAL ENGINEERING JOURNAL, 105, 10 - 15, English
    [Refereed]
    Scientific journal

  • Tomohisa Hasunuma, Jun Ishii, Akihiko Kondo
    Dec. 2015, CURRENT OPINION IN CHEMICAL BIOLOGY, 29, 1 - 9, English
    [Refereed]
    Scientific journal

  • Sayaka Hondo, Masatoshi Takahashi, Takashi Osanai, Mami Matsuda, Tomohisa Hasunuma, Akio Tazuke, Yoichi Nakahira, Shigeru Chohnan, Morifumi Hasegawa, Munehiko Asayama
    Nov. 2015, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 120(5) (5), 510 - 517, English
    [Refereed]
    Scientific journal

  • Nobuko Sumiya, Yasuko Kawase, Jumpei Hayakawa, Mami Matsuda, Mami Nakamura, Atsuko Era, Kan Tanaka, Akihiko Kondo, Tomohisa Hasunuma, Sousuke Imamura, Shin-ya Miyagishima
    Oct. 2015, PLANT AND CELL PHYSIOLOGY, 56(10) (10), 1962 - 1980, English
    [Refereed]
    Scientific journal

  • バイオリファイナリーの現状と展望
    HASUNUMA TOMOHISA, ISHII JUN, OGINO CHIAKI, KONDO AKIHIKO
    Oct. 2015, 化学と生物, 53(10) (10), 689 - 695, Japanese
    Scientific journal

  • 合成生物工学によるモノづくり微生物のデザインに向けて
    ISHII JUN, ARAKI MICHIHIRO, NAKATSUI MASAHIKO, SAKIHAMA YURI, 柘植 陽太, HASUNUMA TOMOHISA, KONDO AKIHIKO
    Sep. 2015, 生物工学会誌, 93(9) (9), 523 - 526, Japanese
    Scientific journal

  • Combined cell-surface display- and secretion-based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae
    LIU Z, INOKUMA Kentaro, HO SHIH-HSIN, den HAAN R, HASUNUMA Tomohisa, van ZYL WH, KONDO Akihiko
    Sep. 2015, Biotechnology for Biofuels, 8, 162, English
    [Refereed]
    Scientific journal

  • Shimpei Aikawa, Shih-Hsin Ho, Akihito Nakanishi, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo
    Jun. 2015, BIOTECHNOLOGY JOURNAL, 10(6) (6), 886 - 898, English
    [Refereed]
    Scientific journal

  • Precipitate obtained following membrane separation of hydrothermally pretreated rice straw liquid revealed by 2D NMR to have high lignin content
    Sasaki Kengo, Okamoto M, Shirai T, Tsuge Yota, Teramura Hiroshi, Sasaki Daisuke, Kawaguchi Hideo, Hasunuma Tomohisa, Ogino Chiaki, Matsuda F, Kikuchi J, Kondo Akihiko
    Jun. 2015, Biotechnology for Biofuels, 8, 88, English
    [Refereed]
    Scientific journal

  • Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Hiroshi Teramura, Kentaro Inokuma, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo
    Jun. 2015, BIORESOURCE TECHNOLOGY, 185, 263 - 268, English
    [Refereed]
    Scientific journal

  • CBPによるバイオエタノール生産技術の開発
    INOKUMA KENTARO, HASUNUMA TOMOHISA, KONDO AKIHIKO
    May 2015, バイオインダストリー, 32(5) (5), 26 - 31, Japanese
    Scientific journal

  • Hideo Kawaguchi, Hiroshi Teramura, Kouji Uematsu, Kiyotaka Y. Hara, Tomohisa Hasunuma, Ko Hirano, Takashi Sazuka, Hidemi Kitano, Yota Tsuge, Prihardi Kahar, Satoko Niimi-Nakamura, Ken-Ichi Oinuma, Naoki Takaya, Shigemitsu Kasuga, Chiaki Ogino, Akihiko Kondo
    Apr. 2015, BIORESOURCE TECHNOLOGY, 182, 169 - 178, English
    [Refereed]
    Scientific journal

  • Evaluation of genes involved in oxidative phosphorylation in yeast by developing a simple and rapid method to measure mitochondrial ATP synthetic activity.
    YE Xiaoting, MORIKAWA Kana, HO SHIH-HSIN, ARAKI Michihiro, NISHIDA Keiji, Hasunuma Tomohisa, HARA Kiyotaka, KONDO Akihiko
    Apr. 2015, Microbial Cell Factories, 14, 56, English
    [Refereed]
    Scientific journal

  • Complete genome sequence of Kluyveromyces marxianus NBRC1777, a nonconventional thermotolerant yeast
    Kentaro Inokuma, Jun Ishii, Kiyotaka Hara, Masao Mochizuki, Tomohisa Hasunuma, Akihiko Kondo
    Apr. 2015, Genome Announcements, 3(2) (2), e00389 - 15, English
    [Refereed]
    Scientific journal

  • 代謝プロファイリングに基づく微生物育種技術の開発と応用
    HASUNUMA TOMOHISA
    Mar. 2015, 生物工学会誌, 93(3) (3), 122 - 129, Japanese
    Scientific journal

  • Yota Tsuge, Tomohisa Hasunuma, Akihiko Kondo
    Mar. 2015, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 42(3) (3), 375 - 389, English
    [Refereed]
    Scientific journal

  • Yuri Sakihama, Tomohisa Hasunuma, Akihiko Kondo
    Mar. 2015, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 119(3) (3), 297 - 302, English
    [Refereed]
    Scientific journal

  • Dynamic metabolic profiling of the marine microalga Chlamydomonas sp. JSC4 and enhancing its oil production by optimizing light intensity
    HO S.H, NAKANISHI A, YE X, CHANG J.S, CHEN C.Y, HASUNUMA Tomohisa, KONDO Akihiko
    Mar. 2015, Biotechnology for Biofuels, 8, 48, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Hyperphosphorylation of DegU cancels CcpA-dependent catabolite repression of rocG in Bacillus subtilis
    Kosei Tanaka, Kana Iwasaki, Takuya Morimoto, Takatsugu Matsuse, Tomohisa Hasunuma, Shinji Takenaka, Onuma Chumsakul, Shu Ishikawa, Naotake Ogasawara, Ken-ichi Yoshida
    Feb. 2015, BMC Microbiology, 15, 43, English
    [Refereed]
    Scientific journal

  • Kentaro Inokuma, Takanobu Yoshida, Jun Ishii, Tomohisa Hasunuma, Akihiko Kondo
    Feb. 2015, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 99(4) (4), 1655 - 1663, English
    [Refereed]
    Scientific journal

  • Chun Wan, Mingming Zhang, Qing Fang, Liang Xiong, Xinqing Zhao, Tomohisa Hasunuma, Fengwu Bai, Akihiko Kondo
    2015, METALLOMICS, 7(2) (2), 322 - 332, English
    [Refereed]
    Scientific journal

  • Ku Syahidah Ku Ismail, Takatoshi Sakamoto, Tomohisa Hasunuma, Xin-Qing Zhao, Akihiko Kondo
    Dec. 2014, BIOTECHNOLOGY JOURNAL, 9(12) (12), 1519 - 1525, English
    [Refereed]
    Scientific journal

  • Shih-Hsin Ho, Xiaoting Ye, Tomohisa Hasunuma, Jo-Shu Chang, Akihiko Kondo
    Dec. 2014, BIOTECHNOLOGY ADVANCES, 32(8) (8), 1448 - 1459, English
    [Refereed]
    Scientific journal

  • Overexpression of flv3 improves photosynthesis in the cyanobacterium Synechocystis sp. PCC6803 by enhancement of alternative electron flow
    HASUNUMA Tomohisa, MATSUDA M, SENGA Y, AIKAWA S, TOYOSHIMA M, SHIMAKAWA G, MIYAKE C, KONDO Akihiko
    Dec. 2014, Biotechnology for Biofuels, 7, 493, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Development of bio-based fine chemical production through synthetic bioengineering
    HARA Kiyotaka, ARAKI Michihiro, OKAI Naoko, WAKAI Satoshi, HASUNUMA Tomohisa, KONDO Akihiko
    Dec. 2014, Microbial Cell Factories, 13(1) (1), 173, English
    [Refereed]
    Scientific journal

  • Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Tomohisa Hasunuma, Takatoshi Sakamoto, Yuri Sakihama, Chiaki Ogino, Akihiko Kondo
    Oct. 2014, BIORESOURCE TECHNOLOGY, 169, 380 - 386, English
    [Refereed]
    Scientific journal

  • Development of a GIN11/FRT-based multiple-gene integration technique affording inhibitor-tolerant, hemicellulolytic, xylose-utilizing abilities to industrial Saccharomyces cerevisiae strains for ethanol production from undetoxified lignocellulosic hemicel
    HASUNUMA Tomohisa, HORI Y, SAKAMOTO Takatoshi, OCHIAI M, HATANAKA H, KONDO Akihiko
    Oct. 2014, Microbial Cell Factories, 13(1) (1), 145, English
    [Refereed]
    Scientific journal

  • Yota Tsuge, Yoshimi Hori, Motonori Kudou, Jun Ishii, Tomohisa Hasunuma, Akihiko Kondo
    Oct. 2014, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98(20) (20), 8675 - 8683, English
    [Refereed]
    Scientific journal

  • Hiroyuki Morita, Tomohisa Hasunuma, Maria Vassileva, Akihiko Kondo, Roumiana Tsenkova
    Sep. 2014, ANALYTICAL METHODS, 6(17) (17), 6628 - 6634, English
    [Refereed]
    Scientific journal

  • アーミング酵母による統合型バイオプロセスの開発
    HASUNUMA TOMOHISA, KONDO AKIHIKO
    Jul. 2014, 日本エネルギー学会誌, 93(7) (7), 580 - 585, Japanese
    Scientific journal

  • Hideo Kawaguchi, Kouji Uematsu, Chiaki Ogino, Hiroshi Teramura, Satoko Niimi-Nakamura, Yota Tsuge, Tomohisa Hasunuma, Ken-Ichi Oinuma, Naoki Takaya, Akihiko Kondo
    Jul. 2014, BIOCHEMICAL ENGINEERING JOURNAL, 88, 188 - 194, English
    [Refereed]
    Scientific journal

  • Optimizing biodiesel production in marine Chlamydomonas sp.JSC4 through metabolic profiling and an innovative salinity-gradient strategy
    HO S.H, NAKANISHI A, YE X, CHANG J.S, HARA Kiyotaka, HASUNUMA Tomohisa, KONDO Akihiko
    Jun. 2014, Biotechnology for Biofuels, 7, 97, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Glycogen production for biofuels by the euryhaline cyanobacteria Synechococcus sp. strain PCC 7002 from an oceanic environment.
    AIKAWA S, NISHIDA A, HO S.H, CHANG J.S, HASUNUMA Tomohisa, KONDO Akihiko
    Jun. 2014, Biotechnology for Biofuels, 7, 88, English
    [Refereed]
    Scientific journal

  • Tomohisa Hasunuma, Ryosuke Yamada, Akihiko Kondo
    Wiley Blackwell, Apr. 2014, Bioprocessing of Renewable Resources to Commodity Bioproducts, 201 - 226, English
    [Refereed]
    In book

  • リグノセルロースからの高効率エタノール生産に向けた新規代謝改変酵母の創製
    HASUNUMA TOMOHISA, KONDO AKIHIKO
    Mar. 2014, オレオサイエンス, 14(3) (3), 95 - 101, Japanese
    Scientific journal

  • Increased biomass production and glycogen accumulation in apcE gene deleted Synechocystis sp. PCC 6803
    JOSEPH A, AIKAWA S, SASAKI Kengo, MATSUDA F, HASUNUMA Tomohisa, KONDO Akihiko
    Mar. 2014, AMB Express, 4, 17, English
    [Refereed]
    Scientific journal

  • Tomohisa Hasunuma, Ku Syahidah Ku Ismail, Yumiko Nambu, Akihiko Kondo
    Feb. 2014, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 117(2) (2), 165 - 169, English
    [Refereed]
    Scientific journal

  • Joseph Ancy, Aikawa Shimpei, Sasaki Kengo, Teramura Hiroshi, Hasunuma Tomohisa, Matsuda Fumio, Osanai Takashi, Hirai Masami Yokota, Kondo Akihiko
    Jan. 2014, FEBS LETTERS, 588(3) (3), 466 - 471
    [Refereed]

  • Ancy Joseph, Shimpei Aikawa, Kengo Sasaki, Hiroshi Teramura, Tomohisa Hasunuma, Fumio Matsuda, Takashi Osanai, Masami Yokota Hirai, Akihiko Kondo
    Jan. 2014, FEBS LETTERS, 588(3) (3), 466 - 471, English
    [Refereed]
    Scientific journal

  • Efficient yeast cell surface display of exo-and endo-cellulase using the SED1 anchoring region and its original promoter
    INOKUMA K, HASUNUMA Tomohisa, KONDO Akihiko
    Jan. 2014, Biotechnology for Biofuels, 7(1) (1), 8, English
    [Refereed]
    Scientific journal

  • Akihito Nakanishi, Shimpei Aikawa, Shih-Hsin Ho, Chun-Yen Chen, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo
    Jan. 2014, BIORESOURCE TECHNOLOGY, 152, 247 - 252, English
    [Refereed]
    Scientific journal

  • Ginga Shimakawa, Tomohisa Hasunuma, Akihiko Kondo, Mami Matsuda, Amane Makino, Chikahiro Miyake
    Japan Society for Bioscience Biotechnology and Agrochemistry, 2014, Bioscience, Biotechnology and Biochemistry, 78(12) (12), 1997 - 2007, English
    [Refereed]
    Scientific journal

  • Yota Tsuge, Toshihiro Tateno, Kengo Sasaki, Tomohisa Hasunuma, Tsutomu Tanaka, Akihiko Kondo
    We produced organic acids, including lactate and succinate, directly from soluble starch under anaerobic conditions using high cell-density cultures of Corynebacterium glutamicum displaying α-amylase (AmyA) from Streptococcus bovis 148 on the cell surface. Notably, reactions performed under anaerobic conditions at 35 and 40°C, which are higher than the optimal growth temperature of 30°C, showed 32% and 19%, respectively, higher productivity of the organic acids lactate, succinate, and acetate compared to that at 30°C. However, α-amylase was not stably anchored and released into the medium from the cell surface during reactions at these higher temperatures, as demonstrated by the 61% and 85% decreases in activity, respectively, from baseline, compared to the only 8% decrease at 30°C. The AmyA-displaying C. glutamicum cells retained their starch-degrading capacity during five 10 h reaction cycles at 30°C, producing 107.8 g/l of total organic acids, including 88.9 g/l lactate and 14.0 g/l succinate. The applicability of cell surface-engineering technology for the production of organic acids from biomass by high cell-density cultures of C. glutamicum under anaerobic conditions was demonstrated.
    Dec. 2013, AMB Express, 3(1) (1), 72 - 72, English, International magazine
    [Refereed]
    Scientific journal

  • Kengo Sasaki, Daisuke Sasaki, Yuri Sakihama, Hiroshi Teramura, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo
    Nov. 2013, BIORESOURCE TECHNOLOGY, 147, 84 - 88, English
    [Refereed]
    Scientific journal

  • Ryosuke Yamada, Tomohisa Hasunuma, Akihiko Kondo
    Nov. 2013, BIOTECHNOLOGY ADVANCES, 31(6) (6), 754 - 763, English
    [Refereed]
    Scientific journal

  • Ku Syahidah Ku Ismail, Takatoshi Sakamoto, Tomohisa Hasunuma, Akihiko Kondo
    Sep. 2013, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 40(9) (9), 1039 - 1050, English
    [Refereed]
    Scientific journal

  • Shih-Hsin Ho, Akihiko Kondo, Tomohisa Hasunuma, Jo-Shu Chang
    Sep. 2013, BIORESOURCE TECHNOLOGY, 143, 163 - 171, English
    [Refereed]
    Scientific journal

  • Hiroko Kato, Fumio Matsuda, Ryosuke Yamada, Kento Nagata, Tomokazu Shirai, Tomohisa Hasunuma, Akihiko Kondo
    Sep. 2013, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 116(3) (3), 333 - 336, English
    [Refereed]
    Scientific journal

  • Yoshihiro Izumi, Shimpei Aikawa, Fumio Matsuda, Tomohisa Hasunuma, Akihiko Kondo
    Jul. 2013, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 930, 90 - 97, English
    [Refereed]
    Scientific journal

  • Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion
    HASUNUMA Tomohisa, KIKUYAMA F, MATSUDA M, AIKAWA S, IZUMI Y, KONDO Akihiko
    Jun. 2013, Journal of Experimental Botany, 64(10) (10), 2943 - 2954, English
    [Refereed]
    Scientific journal

  • Yoko Yamashita, Masaru Yamaoka, Tomohisa Hasunuma, Hitoshi Ashida, Ken-Ichi Yoshida
    May 2013, Journal of Agricultural and Food Chemistry, 61(20) (20), 4850 - 4854, English
    [Refereed]
    Scientific journal

  • Direct conversion of Spirulina to ethanol without pretreatment or enzymatic hydrolysis processes
    AIKAWA S, JOSEPH A, YAMADA R, IZUMI Y, YAMAGISHI T, MATSUDA F, KAWAI H, CHANG JS, HASUNUMA Tomohisa, KONDO Akihiko
    May 2013, Energy & Environmental Science, 6(6) (6), 1844 - 1849, English
    [Refereed]
    Scientific journal

  • Yuki Matano, Tomohisa Hasunuma, Akihiko Kondo
    May 2013, BIORESOURCE TECHNOLOGY, 135, 403 - 409, English
    [Refereed]
    Scientific journal

  • Shih-Hsin Ho, Shu-Wen Huang, Chun-Yen Chen, Tomohisa Hasunuma, Akihiko Kondo, Jo-Shu Chang
    May 2013, BIORESOURCE TECHNOLOGY, 135, 191 - 198, English
    [Refereed]
    Scientific journal

  • Tomohisa Hasunuma, Fumiyoshi Okazaki, Naoko Okai, Kiyotaka Y. Hara, Jun Ishii, Akihiko Kondo
    May 2013, BIORESOURCE TECHNOLOGY, 135, 513 - 522, English
    [Refereed]
    Scientific journal

  • Yuki Matano, Tomohisa Hasunuma, Akihiko Kondo
    Mar. 2013, Applied Microbiology and Biotechnology, 97(5) (5), 2231 - 2237, English
    [Refereed]
    Scientific journal

  • Jun Ishii, Kazuya Yoshimura, Tomohisa Hasunuma, Akihiko Kondo
    Mar. 2013, Applied Microbiology and Biotechnology, 97(6) (6), 2597 - 2607, English
    [Refereed]
    Scientific journal

  • Hiroyuki Suga, Fumio Matsuda, Tomohisa Hasunuma, Jun Ishii, Akihiko Kondo
    Feb. 2013, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(4) (4), 1669 - 1678, English
    [Refereed]
    Scientific journal

  • Kengo Sasaki, Fumio Matsuda, Tomohisa Hasunuma, Chiaki Ogino, Masakatsu Urairi, Kazuhito Yoshida, Akihiko Kondo
    Jan. 2013, Biochemical Engineering Journal, 70, 135 - 139, English
    [Refereed]
    Scientific journal

  • Ku Syahidah Ku Ismail, Takatoshi Sakamoto, Haruyo Hatanaka, Tomohisa Hasunuma, Akihiko Kondo
    Jan. 2013, Journal of Biotechnology, 163(1) (1), 50 - 60, English
    [Refereed]
    Scientific journal

  • Akihiko Kondo, Jun Ishii, Kiyotaka Y. Hara, Tomohisa Hasunuma, Fumio Matsuda
    Jan. 2013, JOURNAL OF BIOTECHNOLOGY, 163(2) (2), 204 - 216, English
    [Refereed]
    Scientific journal

  • Shih-Hsin Ho, Shu-Wen Huang, Chun-Yen Chen, Tomohisa Hasunuma, Akihiko Kondo, Jo-Shu Chang
    Elsevier Ltd, 2013, Bioresource Technology, 135, 157 - 165, English
    [Refereed]
    Scientific journal

  • Tomohisa Hasunuma, Akihiko Kondo
    Nov. 2012, BIOTECHNOLOGY ADVANCES, 30(6) (6), 1207 - 1218, English
    [Refereed]
    Scientific journal

  • バイオリファイナリーとバイオプラスチック
    OKAI NAOKO, HASUNUMA TOMOHISA, KONDO AKIHIKO
    Oct. 2012, ペトロテック, 35(10) (10), 700 - 706, Japanese
    Scientific journal

  • Tomohisa Hasunuma, Akihiko Kondo
    Sep. 2012, PROCESS BIOCHEMISTRY, 47(9) (9), 1287 - 1294, English
    [Refereed]
    Scientific journal

  • Tomohisa Hasunuma, Fumio Matsuda, Akihiko Kondo
    Springer Netherlands, Aug. 2012, Systems Metabolic Engineering, 9789400745346, 329 - 348, English
    [Refereed]
    In book

  • 合成生物工学によるバイオ燃料生産のための微生物細胞工場の創製
    HASUNUMA TOMOHISA, KONDO AKIHIKO
    Jul. 2012, 生物工学会誌, 90(7) (7), 386 - 391, Japanese
    Scientific journal

  • Hiroko Kato, Hiroaki Suyama, Ryosuke Yamada, Tomohisa Hasunuma, Akihiko Kondo
    Jun. 2012, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 94(6) (6), 1585 - 1592, English
    [Refereed]
    Scientific journal

  • Hiroko Kato, Yoshihiro Izumi, Tomohisa Hasunuma, Fumio Matsuda, Akihiko Kondo
    May 2012, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 113(5) (5), 665 - 673, English
    [Refereed]
    Scientific journal

  • Keisuke Fujitomi, Tomoya Sanda, Tomohisa Hasunuma, Akihiko Kondo
    May 2012, BIORESOURCE TECHNOLOGY, 111, 161 - 166, English
    [Refereed]
    Scientific journal

  • Takatoshi Sakamoto, Tomohisa Hasunuma, Yoshimi Hori, Ryosuke Yamada, Akihiko Kondo
    Apr. 2012, JOURNAL OF BIOTECHNOLOGY, 158(4) (4), 203 - 210, English
    [Refereed]
    Scientific journal

  • Synergistic enhancement of glycogen production in Arthrospira platensis by optimization of light intensity and nitrate supply
    AIKAWA Shimpei, IZUMI Yoshihiro, MATSUDA Fumio, HASUNUMA Tomohisa, CHANG J. S, KONDO Akihiko
    Mar. 2012, Bioresource Technology, 108, 211-215, English
    [Refereed]
    Scientific journal

  • Display of cellulases on the cell surface of Saccharomyces cerevisiae affords high yield ethanol production from high-solid lignocellulosic biomass
    MATANO Yuki, HASUNUMA Tomohisa, KONDO Akihiko
    Mar. 2012, Bioresource Technology, 108, 128-133, English
    [Refereed]
    Scientific journal

  • Tomoya Sanda, Tomohisa Hasunuma, Fumio Matsuda, Akihiko Kondo
    Sep. 2011, BIORESOURCE TECHNOLOGY, 102(17) (17), 7917 - 7924, English
    [Refereed]
    Scientific journal

  • Variation in biomass property among rice diverse cultivars.
    MATSUDA Fumio, YAMASAKI Masanori, HASUNUMA Tomohisa, OGINO Chiaki, KONDO Akihiko
    Aug. 2011, Bioscience, Biotechnology, and Biochemistry, 75(8) (8), 1603 - 1605, English
    [Refereed]
    Scientific journal

  • Hiroyuki Morita, Tomohisa Hasunuma, Maria Vassileva, Roumiana Tsenkova, Akihiko Kondo
    Jun. 2011, ANALYTICAL CHEMISTRY, 83(11) (11), 4023 - 4029, English
    [Refereed]
    Scientific journal

  • Tomohisa Hasunuma, Kyung-mo Sung, Tomoya Sanda, Kazuya Yoshimura, Fumio Matsuda, Akihiko Kondo
    May 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 90(3) (3), 997 - 1004, English
    [Refereed]
    Scientific journal

  • Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xyloseo-fermenting strain ofSaccharomyces cerevisiae
    Tomohisa Hasunuma, Tomoya Sanda, Ryosuke Yamada, Kazuya Yoshimura, Jun Ishii, Akihiko Kondo
    Jan. 2011, Microbial Cell Factories, 10(1), 2, English
    [Refereed]
    Scientific journal

  • Takanori Tanino, Atsushi Hotta, Tomonori Ito, Jun Ishii, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Naoto Ohmura, Takayuki Ohshima, Akihiko Kondo
    Nov. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 88(5) (5), 1215 - 1221, English
    [Refereed]
    Scientific journal

  • Construction of a xylose-metabolizing Saccharomyces cerevisiae by integration of xylose isomerase gene into the genome and investigation of the effect of xylITOl on fermentation
    Takanori Tanino, Atsushi Hotta, Tomonori Ito, Jun Ishii, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Naoto Ohmura, Takayuki Ohima, Akihiko Kondo
    Nov. 2010, Applied Microbiology and Biotechnology, 88, 1215-1221, English
    [Refereed]
    Scientific journal

  • Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes.; Applied Microbiology and Biotechnology
    YANASE, Shuhei, HASUNUMA Tomohisa, YAMADA Ryosuke, TANAKA Tsutomu, OGINO Chiaki, FUKUDA Hideki, KONDO Akihiko
    Sep. 2010, Applied Microbiology and Biotechnology, 88(1), 381-388, English
    [Refereed]
    Scientific journal

  • Co-fermentation of cellobiose and xylose using beta-glucosidase displaying diploid industrial yeast strain OC-2
    SAITO, S, HASUNUMA Tomohisa, TANAKA Tsutomu, KONDO Akihiko
    Aug. 2010, Applied Microbiology and Biotechnology, 87(5), 1975-1982, English
    [Refereed]
    Scientific journal

  • Ethanol production from cellulosic materials using cellulase expressing yeast
    YANASE, Shuhei, YAMADA Ryosuke, KANEKO, S, NODA, H, HASUNUMA Tomohisa, TANAKA Tsutomu, OGINO Chiaki, FUKUDA Hideki, KONDO Akihiko
    May 2010, Biotechnology Journal, 5, 449-455, English
    [Refereed]
    Scientific journal

  • Metabolic turnover analysis by a combination of in vivo C-13-lABEling from (CO2)-C-13 and metabolic profiling with CE-MS/MS reveals rate-limiting steps of the C-3 photosynthetic pathwaty in Nicotiana tabacum leaves
    HASUNUMA, Tomohisa, HARADA, K, MIYAZAWA, S, KONDO, Akihiko
    Mar. 2010, Journal of Experimental Botany, 61(4), 1041-1051, English
    [Refereed]
    Scientific journal

  • Metabolic pathway engineering by plastid transformation is a powerful tool for production of compounds in higher plants
    Tomohisa Hasunuma, Akihiko Kondo, Chikahiro Miyake
    Mar. 2009, PLANT BIOTECHNOLOGY, 26(1) (1), 39 - 46, English
    [Refereed]
    Scientific journal

  • Biosynthesis of astaxanthin in tobacco leaves by transplastomic engineering
    HASUNMA Tomohisa, MIYAZAWA Shin-ichi, YOSHIMURA Satomi, SHINZAKI Yuki, TOMIZAWA Kenichi, SHINDO Kazutoshi, CHOI Seon-Kang, MISAWA Norihiko, MIYAKE Chikahiro
    Sep. 2008, The Plant Journal, Vol 55. No. 5, pp. 857-868, English
    [Refereed]
    Scientific journal

  • Overexpression of 1-deoxy-D-xylulose-5-phosphate reductoisomerase gene in chloroplast contributes to increment of isoprenoid production
    HASUNMA Tomohisa, TAKENO Shinya, HAYASHI Shunsuke, SENDAI Mayumi, BAMBA Takeshi, YOSHIMURA Satomi, TOMIZAWA Kenichi, FUKUSAKI Ei-ichiro, MIYAKE Chikahiro
    May 2008, Journal of Bioscience and Biotechnology, Vol 105. No. 5, pp. 518-526, English
    [Refereed]
    Scientific journal

  • 4-Ketoantheraxanthin, a novel carotenoid produced by the combination of the bacterial enzyme b-carotene ketolase CrtW and endogeneous carotenoid biosynthetic enzymes in hihger plants
    SHINDO Kazutoshi, HASUNUMA Tomohisa, ASAGI Emiko, SANO Aya, HOTTA Eri, MINEMURA Noriko, MIYAKE Chikahiro, MAOKA Takashi, MISAWA Norihiko
    Mar. 2008, Tetrahedron Letters, Vol 49. No. 20, pp. 3294-3296, English
    [Refereed]
    Scientific journal

  • Expression of fungal pectin methylesterase in transgenic tobacco leads to alteration cell wall metabolism and a dwarf phenotype
    HASUNMA Tomohisa, FUKUSAKI Ei-ichiro, KOBAYASHI Akio
    Aug. 2004, Journal of Biotechnology, Vol 111. No. 3, pp. 241-251, English
    [Refereed]
    Scientific journal

  • Real-time quantification of methanol in plants using a hybrid alcohol oxidase-peroxidase biosensor
    HASUNMA Tomohisa, FUKUSAKI Ei-ichiro, KUWABATA Susumu, KOBAYASHI Akio
    Mar. 2004, Analytical Chemistry, Vol 76. No. 5, pp. 1500-1506, English
    [Refereed]
    Scientific journal

  • Methanol production is enhanced by expression of an Aspergillus niger pectin methylesterase in tobacco cells
    HASUNMA Tomohisa, FUKUSAKI Ei-ichiro, KOBAYASHI Akio
    Dec. 2003, Journal of Biotechnology, Vol 106. No. 1, pp. 45-52, English
    [Refereed]
    Scientific journal

  • SELEX for tubulin affords specific T-rich DNA aptamers
    FUKUSAKI Ei-ichiro, HASUNMA Tomohisa, KAJIYAMA Shin-ichiro, OKAZAWA Atsushi, ITOH J Tomohiko, KOBAYASHI Akio
    Nov. 2001, Bioorganic & Medicinal Chemistry Letters, Vol. 11, No. 22, pp. 2927-2930, English
    [Refereed]
    Scientific journal

■ MISC
  • 特集 CO2から有用物質を生産し低炭素社会実現へ：AIと代謝工学を組み合わせ生産株開発を加速
    蓮沼誠久
    Jan. 2023, JST news, 1, 10 - 11, Japanese

  • 第4章 生物工学のこれから これからの生物育種 -代謝工学による育種のこれから-
    蓮沼誠久
    Oct. 2022, 日本生物工学会100年史, 58 - 60, Japanese
    Introduction scientific journal

  • 第3章 生物工学の研究100年 育種技術 -突然変異から代謝工学へ-
    近藤昭彦, 蓮沼誠久, 秀瀬涼太
    Oct. 2022, 日本生物工学会100年史, 29 - 31, Japanese
    Introduction scientific journal

  • 微生物の高速育種を実現するスマートセル創出プラットフォーム
    蓮沼誠久, 秀瀬涼太, 番場崇弘
    Apr. 2022, 化学工学 [特集]「生物機能を利用したモノづくり」に貢献するプロセス強化, 86(4) (4), 157 - 160, Japanese
    Introduction scientific journal

  • 進化する酵母細胞表層工学 -バイオインターフェイスの高度利用に向けた技術戦略-
    猪熊健太郎, 蓮沼誠久
    Mar. 2022, オレオサイエンス, 22(3) (3), 99 - 105, Japanese
    Introduction scientific journal

  • 光合成メタボロミクスの物質生産への応用
    加藤悠一, 秀瀬涼太, 蓮沼誠久
    Sep. 2021, 生物工学会誌, 99(9) (9), 456 - 460, Japanese

  • 特集 藻類バイオマス利用のための新しい生物工学
    蓮沼誠久, 新井宗仁
    Aug. 2021, 生物工学会誌, 99(8) (8), 403 - 403, Japanese

  • スマートセルインダストリーの形成に向けたバイオ×デジタルの技術開発
    蓮沼 誠久
    Feb. 2020, 化学工学, 84(2) (2), 51, Japanese
    Introduction scientific journal

  • 新たに設計した代謝経路を用いた大腸菌によるアルカロイド高生産
    蓮沼 誠久, Christopher John Vavricka Jr., 荒木通啓
    Jan. 2020, バイオサイエンスとインダストリー, 78(1) (1), 32 - 33, Japanese
    Introduction scientific journal

  • スマートセル開発に資するメタボロミクス技術
    蓮沼 誠久
    Sep. 2019, バイオサイエンスとインダストリー, 77(5) (5), 410 - 411, Japanese
    Introduction scientific journal

  • 細胞表層提示酵母を利用したクラフトパルプからのキシリトール生産
    番場 崇弘, 猪熊 健太郎, Gregory Guirimand, 蓮沼 誠久
    Sep. 2019, バイオサイエンスとインダストリー, 77(5) (5), 390 - 392, Japanese
    Introduction scientific journal

  • Christopher J Vavricka, Takanobu Yoshida, Yuki Kuriya, Shunsuke Takahashi, Teppei Ogawa, Fumie Ono, Kazuko Agari, Hiromasa Kiyota, Jianyong Li, Jun Ishii, Kenji Tsuge, Hiromichi Minami, Michihiro Araki, Tomohisa Hasunuma, Akihiko Kondo
    Previous studies have utilized monoamine oxidase (MAO) and L-3,4-dihydroxyphenylalanine decarboxylase (DDC) for microbe-based production of tetrahydropapaveroline (THP), a benzylisoquinoline alkaloid (BIA) precursor to opioid analgesics. In the current study, a phylogenetically distinct Bombyx mori 3,4-dihydroxyphenylacetaldehyde synthase (DHPAAS) is identified to bypass MAO and DDC for direct production of 3,4-dihydroxyphenylacetaldehyde (DHPAA) from L-3,4-dihydroxyphenylalanine (L-DOPA). Structure-based enzyme engineering of DHPAAS results in bifunctional switching between aldehyde synthase and decarboxylase activities. Output of dopamine and DHPAA products is fine-tuned by engineered DHPAAS variants with Phe79Tyr, Tyr80Phe and Asn192His catalytic substitutions. Balance of dopamine and DHPAA products enables improved THP biosynthesis via a symmetrical pathway in Escherichia coli. Rationally engineered insect DHPAAS produces (R,S)-THP in a single enzyme system directly from L-DOPA both in vitro and in vivo, at higher yields than that of the wild-type enzyme. However, DHPAAS-mediated downstream BIA production requires further improvement.
    01 May 2019, Nature communications, 10(1) (1), 2015 - 2015, English, International magazine
    Link to Kobe Univ. Repository (Kernel)

  • Branch Spirit
    蓮沼 誠久
    Mar. 2019, 生物工学会誌, 97(3) (3), 150 - 151, Japanese
    Introduction scientific journal

  • チャンスを掴むための準備, バイオ系のキャリアデザイン
    HASUNUMA TOMOHISA
    Oct. 2018, 生物工学会, 96(10) (10), 598 - 601, Japanese
    Introduction scientific journal

  • バイオリファイナリー実現を加速する先端バイオ技術と情報技術の融合
    HASUNUMA TOMOHISA
    Nov. 2017, アグリバイオ, 1(12) (12), 24 - 29, Japanese
    Introduction scientific journal

  • Kentaro Inokuma, Jun Ishii, Kiyotaka Y. Hara, Masao Mochizuki, Tomohisa Hasunuma, Akihiko Kondo
    Feb. 2017, MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 5(5) (5), English
    Others

  • 多彩な戦略で挑むシアノバクテリア由来の燃料生産 持続可能な第三世代バイオ燃料生産の最前線
    日原 由香子, 朝山 宗彦, 蘆田 弘樹, 天尾 豊, 新井 宗仁, 栗井 光一郎, 得平 茂樹, 小山内 崇, 鞆 達也, 成川 礼, 蓮沼 誠久, 増川 一
    日本農芸化学会 ; 1962-, Feb. 2017, 化学と生物, 55(2) (2), 88 - 97, Japanese
    Introduction scientific journal

  • 微細藻類を利用したバイオ燃料とカロテノイド類の生産
    加藤 悠一, 蓮沼 誠久
    Nov. 2016, レーザー研究, 11, 731 - 734, Japanese
    Introduction scientific journal

  • 原子間力顕微鏡による酵母細胞表層セルラーゼの局在評価
    InokumaKentarou, TAKENAKA Musashi, OGINO Chiaki, Hasunuma Tomohisa, KONDO Akihiko
    Nov. 2016, 生物工学, 94(11) (11), 698 - 700, Japanese
    [Refereed]
    Introduction scientific journal

  • 次世代バイオエタノール製造技術
    InokumaKentarou, Hasunuma Tomohisa, KONDO Akihiko
    シーエムシー出版, Oct. 2016, バイオマスエネルギーの技術と市場, 第4章, 33 - 45, Japanese
    [Refereed]
    Introduction scientific journal

  • 代謝プロファイリング法の微生物育種技術への応用
    蓮沼 誠久
    2016, Journal of Environmental Biotechnology, 16(1) (1), 51 - 58, Japanese
    Introduction scientific journal

  • 2S-Ca04 Development of microbial cell factories for biorefinery
    Tomohisa Hasunuma, Akihiko Kondo
    日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 250 - 250, English

  • 好気・低濃度グルコース条件下におけるSaccharomyces cerevisiaeの転写解析
    崎濱由梨, 蓮沼誠久, 蓮沼誠久, 近藤昭彦, 近藤昭彦
    2015, 日本生物工学会大会講演要旨集, 67th

  • Saccharomyces cerevisiaeの糖代謝における転写制御ネットワークの予測
    南部由美子, 南部由美子, 崎濱由梨, 荒木道啓, 蓮沼誠久, 近藤昭彦, 近藤昭彦
    2015, 日本生物工学会大会講演要旨集, 67th

  • 3P-045 Effects of hydrolysate of lignocellulosic biomass on phenyllactate fermentation by recombinant Escherichia coli
    Kawaguchi Hideo, Teramura Hiroshi, Niimi-Nakamura Satoko, Ogino Chiaki, Hara Kiyotaka, Hasunuma Tomohisa, Oinuma Ken-Ichi, Takaya Naoki, Hirano Ko, Sazuka Takashi, Kitano Hidemi, Kondo Akihiko
    日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 206 - 206, Japanese

  • 3P-044 Improvement of CHO cells useful in bio-pharmaceutical development, construction of data leading to the culture state elucidation
    Iga Tomoyo, Hasunuma Tomohisa, Araki Michihiro, Kondo Akihiko
    日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 205 - 205, Japanese

  • 1P-134 Development of the novel secretion signal sequence for highly-efficient cell surface display and secretory production of proteins by yeasts
    Inokuma Kentaro, Hasunuma Tomohisa, Kondo Akihiko
    日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 51 - 51, Japanese

  • 3P-205 Metabolite profiling of oceanic cyanobacteria under salt stress condition
    Nishida Atsumi, Aikawa Shimpei, Hasunuma Tomohisa, Kondo Akihiko
    日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 246 - 246, Japanese

  • 3P-229 Effect of PHO13 gene disruption on xylose production in xylose-fermenting yeast harboring xylose isomerase gene
    Bamba Takahiro, Hasunuma Tomohisa, Kondo Akihiko
    日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 252 - 252, Japanese

  • 2P-132 Development of lipid-producing system depending on estimation of cell composition and metabolic analysis for marine green alga Chlamydomonas sp. JSC4
    Nakanishi Akihito, Ho Shih-Hsin, Chang Jo-Shu, Hasunuma Tomohisa, Kondo Akihiko
    日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 139 - 139, Japanese

  • 2P-136 Effect of illumination coupled with nitrogen depletion on biodiesel production of a marine microalga Chlamydomonas sp. JSC4 :
    Ho Shih-Hsin, Nakanishi Akihito, Ye Xiaoting, Chang Jo-Shu, Hara Kiyotaka, Kondo Akihiko, Hasunuma Tomohisa
    日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 140 - 140, English

  • 2P-095 High-level ethanol production from cyanobacterium Spirulina by amylase displayed yeast
    Aikawa Shimpei, Inokuma Kentaro, Sasaki Kengo, Hasunuma Tomohisa, Kondo Akihiko
    日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 130 - 130, Japanese

  • 2A-Dp01 Development of microbe breeding technology based on metabolic profiling
    Hasunuma Tomohisa
    日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 98 - 98, Japanese

  • 1P-110 Detoxification of furfural in Corynebacterium glutamicum under aerobic and anaerobic conditions
    Tsuge Yota, Hori Yoshimi, Hasunuma Tomohisa, Kondo Akihiko
    日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 44 - 44, Japanese

  • 3S-Ba05 Challenge for liquid fuel production from algae based on metabolic analysis
    Hasunuma Tomohisa
    日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 177 - 177, Japanese

  • キシロース資化性酵母における遺伝子発現プロファイルの経時変化解析
    南部由美子, 崎濱由梨, 蓮沼誠久, 蓮沼誠久, 近藤昭彦, 近藤昭彦
    2014, 日本生物工学会大会講演要旨集, 66th

  • Kluyveromyces marxianusの好気条件での糖代謝系の解析
    崎濱由梨, 蓮沼誠久, 蓮沼誠久, 近藤昭彦, 近藤昭彦
    2014, 日本生物工学会大会講演要旨集, 66th

  • バイオリファイナリー社会に向けた燃料・化学品生産
    ISHII JUN, HASUNUMA TOMOHISA, MATSUDA FUMIO, KONDO AKIHIKO
    Aug. 2013, 安全工学, 52(4) (4), 249 - 255, Japanese
    Introduction scientific journal

  • 革新的なものづくり実現のための「合成生物工学」
    ISHII JUN, HASUNUMA TOMOHISA, MATSUDA FUMIO, KONDO AKIHIKO
    日本生物工学会, Jun. 2013, 生物工学会誌, 91(6) (6), 314 - 318, Japanese
    Introduction scientific journal

  • 1P-104 Identification of genes related to improved ethanol production from xylose in presence of acetate and formate by regulation of metabolism in Saccharomyces cerevisiae
    Sakamoto Takatoshi, Hori Yoshimi, Hasunuma Tomohisa, Kondo Akihiko
    日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 43 - 43, Japanese

  • 1P-185 Development of high lipid producing system by green alga Chlamydomonas orbicularis under sea salt condition
    Nakanishi Akihito, Ho Shih-Hsin, Aikawa Shimpei, Chang Jo-Shu, Kondo Akihiko, Hasunuma Tomohisa
    日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 64 - 64, Japanese

  • 1P-196 Phototrophic cultivation of a marine microalga Chlamydomonas orbicularis for CO_2 fixation and biodiesel production: Effect of medium composition, nitrogen depletion, and sea salt concentration :
    Ho Shih-Hsin, Nakanishi Akihito, Aikawa Shimpei, Chang Jo-Shu, Kondo Akihiko, Hasunuma Tomohisa
    日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 66 - 66, English

  • 1P-186 Glycogen production for biorefinery using oceanic cyanobacteria
    Nishida Atsumi, Aikawa Shimpei, Hasunuma Tomohisa, Kondo Akihiko
    日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 64 - 64, Japanese

  • 1P-146 Improvement of ethanol production from xylose by regulation of the PHO pathway in Saccharomyces cerevisiae
    Hasunuma Tomohisa, Kondo Akihiko
    日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 54 - 54, Japanese

  • 1P-191 The effect of sea-salts on the cell-growth and photosynthesis in a remarkable carbon feedstock Spirulina
    Aikawa Shimpei, Akimoto Seiji, Hasunuma Tomohisa, Kondo Akihiko
    日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 65 - 65, Japanese

  • 1P-184 Development of novel yeast cell surface display system for consolidated bioethanol production from lignocellulosic biomass
    Inokuma Kentaro, Hasunuma Tomohisa, Kondo Akihiko
    日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 63 - 63, Japanese

  • 2P-148 Sugar concentration in liquid hydrolysate of dilute acid pretreated rice straw by nanofiltration
    Sasaki Kengo, Hasunuma Tomohisa, Ogino Chiaki, Kondo Akihiko
    日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 141 - 141, Japanese

  • 統合バイオプロセスによる環境調和型セルロース系エタノール生産に資する前処理技術の開発
    崎濱由梨, 俣野結城, 蓮沼誠久, 蓮沼誠久, 近藤昭彦, 近藤昭彦
    2013, 日本生物工学会大会講演要旨集, 65th

  • 発酵阻害物存在下におけるキシロースからのエタノール発酵向上酵母の構築
    崎濱由梨, 蓮沼誠久, 近藤昭彦
    2013, 日本農芸化学会大会講演要旨集(Web), 2013

  • Cyanobacterium Synechocystis sp. PCC6803 におけるO2存性オルタナティブ・エレクトロン・フロー (AEF)の生理的役割
    林 良祐, 清水 聡子, 山本 宏, 藍川 晋平, 蓮沼 誠久, 秋本 誠志, 近藤 昭彦, 三宅 親弘
    Mar. 2012, 第53回日本植物生理学会, Japanese

  • 2Gp16 Metabolome analysis of filamentous fungus Rhizopus oryzae showing different behaviors of lipase secretion
    Yoshida Ayumi, Hama Shinji, Hasunuma Tomohisa, Yamamoto Hiroyuki, Kondo Akihiko
    日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 65 - 65, Japanese

  • 有用希少イノシトールのバイオアベイラビリティーとバイオコンバージョン生産
    吉田 健一, 蓮沼 誠久
    日本生物工学会, Oct. 2011, 生物工学会誌, 89(10) (10), 585 - 588, Japanese
    Introduction scientific journal

  • ＣＢＰ用スーパー酵母を用いたバイオエタノール生産技術の開発
    HASUNUMA TOMOHISA, OGINO CHIAKI, KONDO AKIHIKO
    Oct. 2011, ブレインテクノニュース, 144, 17 - 22, Japanese
    Introduction scientific journal

  • バイオマスからの効率的なバイオ燃料の生産技術 (特集 本格化するバイオマスエネルギー開発)
    蓮沼 誠久, 近藤 昭彦
    化学工業社, Sep. 2011, ケミカルエンジニヤリング, 56(9) (9), 661 - 668, Japanese

  • バイオマスからの効率的なバイオ燃料の生産技術
    HASUNUMA TOMOHISA, KONDO AKIHIKO
    Sep. 2011, ケミカルエンジニヤリング, 56(9) (9), 5 - 12, Japanese
    Introduction scientific journal

  • 11 シアノバクテリアでのオルタナティブ・エレクトロン・フロー(AEF)評価系の確立(関西支部講演会,2010年度各支部会講演要旨)
    進藤 沙織, 林 吉祐, 真野 陽人, 杉本 敏男, 近藤 昭彦, 藍川 晋平, 蓮沼 誠久, 秋本 誠志, 三宅 親弘
    一般社団法人日本土壌肥料学会, 08 Aug. 2011, 日本土壌肥料学会講演要旨集, (57) (57), 329 - 329, Japanese

  • 微細藻類によるバイオリファイナリー
    HASUNUMA TOMOHISA, 藍川 晋平, 和泉 自泰, KONDO AKIHIKO
    Apr. 2011, 生物工学会誌, 89(4) (4), 181 - 183, Japanese
    Introduction scientific journal

  • 2Ip14 High efficient ethanol production from hydrothermally-processed rice straw using cellulase-displaying yeast with a rotary fermenter
    Matano Yuki, Hasunuma Tomohisa, Kondo Akihiko
    日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 180 - 180, Japanese

  • 2Ka04 Reduction of furan derivatives by NADH-dependent Adh1 improves ethanol fermentation using xylose as sole carbon source with Saccharomyces cerevisiae harboring XR-XDH pathway
    Ishii Jun, Yoshimura Kazuya, Hasunuma Tomohisa, Kondo Akihiko
    日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 187 - 187, Japanese

  • バイオマスからのバイオ燃料生産に向けた戦略
    KONDO Akihiko, OGINO Chiaki, HASUNUMA Tomohisa, TANAKA Tsutomu, NAKASHIMA Kazunori
    Nov. 2010, バイオインダストリー, 11, 6-12, Japanese
    Introduction scientific journal

  • 次世代燃料・化成品原料製造に向けたバイオリファイナリー戦略
    KONDO Akihiko, OGINO Chiaki, HASUNUMA Tomohisa, TANAKA Tsutomu, NAKASHIMA Kazunori
    Jul. 2010, 生物工学, 88(7), 333-335, Japanese
    Introduction scientific journal

  • 2-IV-14 D-アラビノースは酵母エリスロアスコルビン酸の内在性前駆体ではない(一般演題,日本ビタミン学会第62回大会発表要旨)
    尼子 克己, 森村 健一, 蓮沼 誠久, 近藤 昭彦, 合田 清
    日本ビタミン学会, 25 Apr. 2010, ビタミン, 84(4) (4), 206 - 206, Japanese

  • 3P-1119 Efficient ethanol production from xylose in the presence of fermentation inhibitors
    SANDA Tomoya, YAMADA Ryosuke, HASUNUMA Tomohisa, KONDO Akihiko
    日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 87 - 87, Japanese

  • 3S-Ba04 Development of bio-fuel production system using microalgae
    KONDO Akihiko, HASUNUMA Tomohisa
    日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 202 - 202, Japanese

  • 2P-2051 Efficient fermentation of xylose to ethanol in the presence of furan compound by recombinant Saccharomyces cerevisiae expressing Alcohol dehydrogenase
    YOSHIMURA Kazuya, ISHII Jun, HASUNUMA Tomohisa, KONDO Akihiko
    日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 119 - 119, Japanese

  • 3P-1011 Construction of the recombinant xylose fermentation yeast with high β-xylosidase activity from OC-2 triple marker strain as a platform
    Saitoh Satoshi, Tanaka Tsutomu, Hasunuma Tomohisa, Kondo Akihiko
    日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 60 - 60, Japanese

  • Akihiko Kondo, Tsutomu Tanaka, Tomohisa Hasunuma, Chiaki Ogino
    2010, Recent Patents on Biotechnology, 4(3) (3), 226 - 234, English
    [Refereed]
    Introduction scientific journal

  • SB-O5 Dynamic metabolic profiling in plant leaves using in vivo stable isotope labeling(Section XI Systems Biotechnology/Metabolic Engineering)
    Hasunuma Tomohisa, Kondo Akihiko, Fukusaki Eiichiro
    The Society for Biotechnology, Japan, Nov. 2009, Journal of bioscience and bioengineering, 108(1) (1), S167, English

  • Tomohisa Hasunuma, Akihiko Kondo, Eiichiro Fukusaki
    Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S167 - S167, English
    Summary international conference

  • Tanino Takanori, Hotta Atsushi, Ito Tomonori, Ishii Jun, Yamada Ryosuke, Hasunuma Tomohisa, Ogino Chiaki, Ohmura Naoto, Ohshima Takayuki, Kondo Akihiko
    Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(1) (1), S53, English
    [Refereed]
    Summary international conference

  • Hotta Atsushi, Tanino Takanori, Ito Tomonori, Hasunuma Tomohisa, Ogino Chiaki, Kondo Akihiko, Ohmura Naoto
    Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S53, English
    [Refereed]
    Summary international conference

  • バイオマスからの化学品生産を目指したバイオプロセスの開発
    HASUNUMA Tomohisa, OKANO Kenji, TATENO Toshihiro, KONDO Akihiko
    Mar. 2009, ケミカルエンジニヤリング, Vol 54. No. 3, pp. 24-30, Japanese
    Introduction scientific journal

  • 3S15a04 Bioproduction through synthetic bioengineering
    KONDO Akihiko, FUKUSAKI Eiichiro, HASUNUMA Tomohisa
    日本生物工学会, 2009, 日本生物工学会大会講演要旨集, 21, 292 - 292, Japanese

  • 13CO2を用いた同位体標識による葉内代謝物質のターンオーバー解析
    蓮沼誠久, 原田和生, 三宅親弘, 福崎英一郎
    2008, 生化学

  • Dynamic metabolic profiling using in vivo stable isotope labeling
    蓮沼 誠久, 原田 和生, 三宅 親弘, 福崎 英一郎
    生体試料に含まれる代謝産物の量的情報をMSやNMRを利用して網羅的に解析する代謝プロファイリング技術は，微量な中間代謝物質を含む多様な化合物の一斉分析を可能にしたが，ここで得られる情報は代謝物質を抽出した時点での蓄積量のスナップショットであった．生体内で代謝物質が動的定常状態にある時，総量は変わらずに一定の同じ速度で分解と合成が行われて入れ替わっており，新規合成される物質の存在比率を知るためには安定同位体を用いたin vivo標識が必須である．代謝プロファイリングと安定同位体標識法の組合せを利用した「動的代謝プロファイリング」は，代謝産物の量的変動を網羅的に観測することを可能にする．植物の環境変化やストレスに対する応答はシステマティックな代謝変動に基づくことが予想され，「動的代謝プロファイリング」はこうした複雑な応答機構の解明に寄与することが期待できる．Metabolic profiling technique has enabled to analyze the level of various metabolites including minor intermediates by using MS or NMR simultaneously, but the information available from the technique was snapshot in vivo at the time of sampling metabolites. In the dynamic steady state, metabolites are replaced with newly-synthesized compounds at a constant rate and the total amount of metabolites remains unchanged. Therefore, in vivo labeling with stable isotopes is necessary for the determination of turnover rates of metabolites. Dynamic metabolic profiling formed by the combination of metabolic profiling and stable isotope labeling technique enables to determine changes in metabolic flux exhaustively. In plants, responses to stress or environmental changes would be based on systematic variations in metabolism. The dynamic metabolic profiling would contribute to elucidate the complicated response mechanism.光合成研究法. 北海道大学低温科学研究所, 日本光合成研究会共編
    北海道大学低温科学研究所 = Institute of Low Temperature Science, Hokkaido University, 2008, Low temperature science, 67, 169 - 174, Japanese

  • Expression of Aspergillus niger pectin methylesterase in transgenic tobacco (Nicotiana tabacum) leads to alterations on the level of methanol and a dwarf phenotype
    Hasunuma Tomohisa, Fukusaki Eiichiro, Okazawa Atsushi, Kajiyama Shinichiro, Kobayashi Akio
    公益社団法人日本生物工学会, 25 Aug. 2003, 日本生物工学会大会講演要旨集, 15, Japanese

  • Hasunuma Tomohisa, Fukusaki Ei-ichiro, Okazawa Atsushi, Kajiyama Shin-ichiro, Kobayashi Akio
    公益社団法人日本生物工学会, 10 Jul. 2000, 日本生物工学会大会講演要旨集, 12, Japanese

■ Books And Other Publications
  • クリーンエネルギーの技術と市場 2022
    猪熊健太郎, 蓮沼誠久
    Joint work, 第9章 バイオマス燃料生産の技術動向, シーエムシー出版, Feb. 2022

  • 醸造の辞典
    蓮沼誠久
    第2章 醸造の科学, 最近の育種技術, 朝倉書店, Jun. 2021

  • バイオリアクターのスケールアップと物質生産事例集
    蓮沼誠久
    第11章 スマートセルの動向と効率的創製に向けたAI活用, 第1節 国内におけるスマートセルの動向, 技術情報協会, Apr. 2021

  • メタボロミクス 実践ガイド
    蓮沼誠久
    応用・展望編 Ⅰ. 応用研究 代謝工学分野へのメタボロミクスの応用, 羊土社, Apr. 2021

  • Carotenoids: Biosynthetic and Biofunctional Approaches
    Yuichi Kato, Tomohisa Hasunuma
    Metabolic Engineering for Carotenoid Production Using Eukaryotic Microalgae and Prokaryotic Cyanobacteria, Springer, 2021

  • 脱石油に向けたCO2資源化技術―化学・生物プロセスを中心に―
    蓮沼誠久
    Joint work, 第Ⅲ編 生物プロセス, 第2章 ラン藻によるバイオコハク酸生産, シーエムシー, Jul. 2020

  • 骨格筋研究を核とした筋スマート社会
    西村 勇哉, 蓮沼 誠久
    Joint work, 第3章 7. 組織内の分子を網羅的に測る技術, シーエムシー・リサーチ, Jun. 2019

  • 第9章 油脂酵母による油脂発酵生産性改善へ向けた技術開発, スマートセルインダストリー -微生物細胞を用いた物質生産の展望-, 第3編 産業応用へのアプローチ
    高久 洋暁, 荒木 秀雄, 小笠原 渉, 田代 康介, 蓮沼 誠久, 油谷 幸代, 矢追 克郎
    Joint work, シーエムシー出版, Jun. 2018, Japanese
    Scholarly book

  • スマートセル設計に資するメタボローム解析, スマートセルインダストリー -微生物細胞を用いた物質生産の展望-, 第1編 ハイスループット合成・分析・評価技術, 第3章 オミクス解析技術
    蓮沼 誠久
    Joint work, シーエムシー出版, Jun. 2018, Japanese
    Scholarly book

  • スマートセルインダストリー –微生物細胞を用いた物質生産の展望–（第1編 ハイスループット合成・分析・評価技術，第2章 ハイスループット微生物構築・評価技術，第1節 「微生物を用いた物質生産とハイスループット微生物構築技術」）
    ISHII JUN, 西 晶子, 北野 美保, 中村 朋美, SHOUJI SHINICHIROU, HIDESE RYOTA, HASUNUMA TOMOHISA, KONDO AKIHIKO
    Joint work, シーエムシー出版, Jun. 2018, Japanese
    Scholarly book

  • スマートセルインダストリー, 総論 -微生物細胞を用いた物質生産の展望-
    HASUNUMA TOMOHISA, 田村 具博, KONDO AKIHIKO
    Joint work, シーエムシー出版, Jun. 2018, Japanese
    Scholarly book

  • Emerging Areas in Bioengineering：Whole cell biocatalysts using enzymes displayed on yeast cell surface
    Kentaro Inokuma, Tomohisa Hasunuma, Akihiko Kondo
    Joint work, Wiley-VCH, Feb. 2018, English
    Scholarly book

  • Industrial Biotechnoligy : Products and Processes, Chapter 5, Production of fuels and chemicals from biomass by integrated bioprocesses
    HASUNUMA Tomohisa, KONDO Akihiko
    Joint work, Wley, Nov. 2016, English
    Scholarly book

  • 応用微生物学，第10章 低炭素化社会への取組み，バイオ燃料
    蓮沼 誠久
    Joint work, 文永堂出版, Aug. 2016, Japanese
    Scholarly book

  • Bioprocessing of Renewable Resources to Commodity Bioproducts, Chapter 8, Ethanol production from yeasts
    HASUNUMA Tomohisa, YAMADA Ryosuke, KONDO Akihiko
    Joint work, Wiley, Apr. 2014, English
    Scholarly book

  • 化学便覧 応用化学編
    KONDO AKIHIKO, OGINO CHIAKI, HASUNUMA TOMOHISA, ISHII JUN, HARA KIYOTAKA, OKAI NAOKO, 山田 亮祐
    Joint work, 丸善出版, Jan. 2014, Japanese
    Scholarly book

  • 次世代パワートレイン開発と燃料技術
    HASUNUMA TOMOHISA, KONDO AKIHIKO
    Joint work, シーエムシー出版, Nov. 2013, Japanese
    Scholarly book

  • リサイクルバイオテクノロジーの最前線, 第I編 第1章, 8 微細藻類・シアノバクテリアからのバイオエタノール生産
    HASUNUMA TOMOHISA
    Joint work, シーエムシー出版, May 2013, Japanese
    Scholarly book

  • リサイクルバイオテクノロジーの最前線, 第I編 第1章, 1 未来型CBP法によるバイオエタノール生産技術の展開
    HASUNUMA TOMOHISA, OGINO CHIAKI, KONDO AKIHIKO
    Joint work, シーエムシー出版, May 2013, Japanese
    Scholarly book

  • ひらく、ひらく「バイオの世界」
    崎濱 由梨, HASUNUMA TOMOHISA, KONDO AKIHIKO
    Joint work, 化学同人, Oct. 2012, Japanese
    Scholarly book

  • 微細藻類によるエネルギー生産と事業展望
    HASUNUMA TOMOHISA, KONDO AKIHIKO
    Joint work, シーエムシー出版, Jul. 2012, Japanese
    Scholarly book

  • 藻類ハンドブック
    HASUNUMA TOMOHISA, KONDO AKIHIKO
    Joint work, エヌ・ティー・エス, Jul. 2012, Japanese
    Scholarly book

  • 合成生物工学の隆起
    HASUNUMA TOMOHISA, KONDO AKIHIKO
    Joint work, シーエムシー出版, Apr. 2012, Japanese
    Scholarly book

  • 微生物機能学
    HASUNUMA TOMOHISA, KONDO AKIHIKO
    Joint work, 三共出版, Mar. 2012, Japanese
    Scholarly book

  • バイオマス分解酵素研究の最前線, 第7編, 第28章 セルラーゼ細胞表層提示酵母を用いたバイオマス変換
    山田 亮祐, 蓮沼 誠久, 近藤 昭彦
    Joint work, シーエムシー出版, Mar. 2012, Japanese
    Scholarly book

  • バイオマス分解酵素研究の最前線, 序章, 2 バイオマス分解酵素研究の新たな展開
    蓮沼 誠久, 近藤 昭彦
    Joint work, シーエムシー出版, Mar. 2012, Japanese
    Scholarly book

  • System Metabolic Engineering
    HASUNUMA Tomohisa, MATSUDA Fumio, KONDO Akihiko
    Joint work, Springer, 2012, English
    Scholarly book

  • エコバイオリファイナリー
    KONDO Akihiko, OGINO Chiaki, HASUNUMA Tomohisa, TANAKA Tsutomu, NAKASHIMA Kazunori
    Joint work, シーエムシー出版, Dec. 2010, Japanese
    Scholarly book

  • グリーンバイオケミストリーの最前線
    OGINO Chiaki, HASUNUMA Tomohisa, KONDO Akihiko
    Joint work, シーエムシー出版, Apr. 2010, Japanese
    Scholarly book

  • Methods in Molecular Biology
    蓮沼 誠久, 近藤 昭彦, 三宅 親弘
    Joint work, Humana Press, Feb. 2010, English
    Scholarly book

  • 次世代バイオエタノール生産の技術革新と事業展開, 第7章, ４ アーミング酵母を用いた統合プロセスによるバイオエタノール生産
    蓮沼 誠久, 山田 亮祐, 近藤 昭彦
    Joint work, フロンティア出版, Jan. 2010, Japanese
    Scholarly book

  • 次世代バイオエタノール生産の技術革新と事業展開, 第10章, 7 アーミング技術を応用した新規セルロース分解システムの構築
    山田 亮祐, 蓮沼 誠久, 近藤 昭彦
    Joint work, フロンティア出版, Jan. 2010, Japanese
    Scholarly book

  • 第二世代バイオ燃料の開発と応用展開
    近藤 昭彦, 福田 秀樹, 荻野 千秋, 田中 勉, 蓮沼 誠久, 原田 和生, 福崎 英一郎
    Joint work, シーエムシー出版, Apr. 2009, Japanese
    Scholarly book

  • 水環境の今と未来 藻類と植物のできること
    HASUNMA Tomohisa, KONDO Akihiko
    Joint work, 生物研究社, Mar. 2009, Japanese
    General book

  • 光合成研究法
    HASUNMA Tomohisa, HARADA Kazuo, MIYAKE Chikahiro, FUKUSAKI Ei-ichiro
    Joint work, 北海道大学低温科学研究所・日本光合成研究会共編, Mar. 2009, Japanese
    Scholarly book

■ Lectures, oral presentations, etc.
  • ポストコロナ時代の産学連携について考える
    蓮沼誠久
    レーザー学会学術講演会第41回年次大会シンポジウム, Jan. 2021, Japanese

  • バイオプロセスにおける膜利用の現状と将来展望
    蓮沼誠久
    第一回先端膜工学研究推進機構特定テーマフォーラム～医薬・バイオプロセスにおける膜利用の現状と将来展望～, Dec. 2020, Japanese

  • Metabolomics-based engineering biology for microbial bio-production with sustainability
    Tomohisa Hasunuma
    2nd ASBA Webinar, Dec. 2020, English

  • ハイスループットプラットフォームの開発による微生物スマートセルの構築
    蓮沼誠久
    CBI学会2020年大会, Oct. 2020, Japanese

  • 微細藻類を利用した物質生産プロセスへのLEDの応用
    蓮沼誠久, 加藤悠一
    JPC関西特別報告会, Oct. 2020, Japanese

  • メタボローム解析の自動化に向けた挑戦
    蓮沼誠久
    Laboratory Automation勉強会, Aug. 2020, Japanese

  • バイオ×デジタルの技術融合による有用微生物「スマートセル」開発への挑戦
    蓮沼誠久
    質量分析インフォマティクス研究会 第５回ワークショップ, Aug. 2020, Japanese
    [Invited]

  • スマートセルインダストリーに資するハイスループット微生物構築・評価技術の開発
    蓮沼 誠久
    日本農芸化学会2020年度大会, Japanese

  • スマートセル開発に資するハイスループット微生物構築・評価技術の概要
    蓮沼 誠久
    NEDOフェスタin関西 2019, Dec. 2019, Japanese, Domestic conference
    [Invited]
    Public discourse

  • 酵母Saccharomyces cerevisiaeの鉄代謝改変に基づくキシロースからの1,2,4-ブタントリオール発酵生産
    湯川貴弘, 番場崇弘, 蓮沼誠久, 近藤昭彦
    日本農芸化学会関西支部 支部例会（第511回講演会）, Dec. 2019, Japanese, Domestic conference
    Oral presentation

  • スマートセルインダストリーに資するメタボローム解析技術の開発と応用
    蓮沼 誠久
    日本農芸化学会関西支部 支部例会（第511回講演会）, Dec. 2019, Japanese, Domestic conference
    [Invited]
    Invited oral presentation

  • 藍藻の動的メタボローム解析技術の開発と応用
    蓮沼 誠久
    藍藻の分子生物学2019, Nov. 2019, Japanese, Domestic conference
    [Invited]
    Invited oral presentation

  • バイオ×デジタルによるスマートセル開発への挑戦
    蓮沼 誠久
    Bio Japan 2019, Oct. 2019, Japanese, Domestic conference
    [Invited]
    Nominated symposium

  • Metabolomics-based engineering for smart cell development
    Tomohisa Hasunuma
    Metabolic Engineering Summit 2019, Oct. 2019, English, International conference
    Oral presentation

  • Metabolomics-based engineering biology for bio-production
    Tomohisa Hasunuma
    2019 Asian Synthetic Biology Association (ASBA) Meeting, Oct. 2019, English, International conference
    Oral presentation

  • Re-design, synthesis, and bio-analysis of sulfosialic acids as covalent inhibitors of neuraminidase
    ヴァヴリッカ クリストファー, 松本達磨, 荒木通啓, 蓮沼誠久, 泉実, 清田洋正
    第61回天然有機化合物討論会, Sep. 2019, English, Domestic conference
    Poster presentation

  • スマートセル開発に資するハイスループット微生物構築・評価技術の概要
    蓮沼 誠久
    2019年度NEDOスマートセルプロジェクト技術セミナー, Sep. 2019, Japanese, Domestic conference
    Invited oral presentation

  • 窒素源存在下で油脂を高蓄積する海洋性クラミドモナス変異株の選択的育種
    OYAMA TOMOKI, KATO YUICHI, 佐藤 勝也, 大野 豊, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference
    Oral presentation

  • 食農産業における LED の利活用技術の開発
    藤川 康夫, 鶴本 智大, 泉田 智史, 三島 俊介, 岡澤 敦司, KATO YUICHI, HASUNUMA TOMOHISA
    第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference
    Oral presentation

  • 細胞表層提示システムに適した宿主酵母の開発
    INOKUMA KENTARO, KITADA YUKI, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference
    Oral presentation

  • 海洋性クラミドモナス油脂高蓄積変異株における油脂・カロテノイド増産メカニズムの解明
    KATO YUICHI, OYAMA TOMOKI, 佐藤 勝也, 大野 豊, 張 嘉修, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference
    Oral presentation

  • Pichia pastorisにおいて分泌シグナル配列の１アミノ酸置換はタンパク質分泌生産量を劇的に増大させる
    ITO YOICHIRO, 石上 美佐, 橋場 倫子, NAKAMURA YASUYUKI, HASUNUMA TOMOHISA, ISHII JUN, KONDO AKIHIKO
    第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学 津島キャンパス, Domestic conference
    Oral presentation

  • Smart Cell Development Based on High Throughput Technology, Advanced Analysis and Computational Science for Bio-production in Microorganisms
    Tomohisa Hasunuma
    The 14th Asian Congress on Biotechnology (ACB2019), Jul. 2019, English, International conference
    [Invited]
    Invited oral presentation

  • 酵母 Scheffersomyces stipitisを用いたバイオマス資源からの有用芳香族化合物生産プロセスの開発
    小林 優真, 猪熊 健太郎, 蓮沼 誠久, 近藤 昭彦
    生物工学若手研究者の集い 夏のセミナー2019, Jul. 2019, Japanese, Domestic conference
    Poster presentation

  • Metabolomics-based engineering biology of microorganisms
    Tomohisa Hasunuma
    Metabolomics 2019, Jun. 2019, English, International conference
    Oral presentation

  • スマートセル創出プラットフォーム構築へ向けた挑戦
    HASUNUMA TOMOHISA
    第6回SBJシンポジウム, May 2019, Japanese, 千里ライフサイエンスセンター, Domestic conference
    Oral presentation

  • 緑色LEDを用いた微細藻類バイオマス増産技術の開発
    加藤 悠一, 藤川 康夫, 三島 俊介, 泉田 智史, 蓮沼 誠久, 近藤 昭彦
    日本農芸化学会2019年度大会, Mar. 2019, Japanese, 東京農業大学 世田谷キャンパス, Domestic conference
    Poster presentation

  • 4-アミノフェニルアラニン発酵における組換え大腸菌の代 謝改変
    川口 秀夫, 若井 桂子, 蓮沼 誠久, 桝尾 俊介, 高谷 直樹, 近藤 昭彦
    日本農芸化学会2019年度大会, Mar. 2019, Japanese, 東京農業大学 世田谷キャンパス, Domestic conference
    Poster presentation

  • スマートセル創出プラットフォームの構築と実証に向けて
    蓮沼 誠久
    関西スマートセルフォーラム2018 第3回セミナー, Jan. 2019, Japanese, 大阪中之島センター, Domestic conference
    Oral presentation

  • Platform Technologies for Development of Smart Cell
    TOMOHISA Hasunuma
    Asian Synthetic Biology Association 2018, Nov. 2018, English, Hyatt Regency Jeju, International conference
    Oral presentation

  • Development of "smart cell" construction platform for next-generation microbial breeding
    TOMOHISA Hasunuma
    IEE CPMT Symposium Japan 2018, Nov. 2018, English, 京都大学, International conference
    [Invited]
    Invited oral presentation

  • Construction of a stable, autonomously replicating plasmid vector containing Pichia pastoris centromeric DNA
    NAKAMURA YASUYUKI, Teruyuki Nishi, Risa Noguchi, ITO YOICHIRO, Toru Watanabe, Tozo Nishiyama, Shimpei Aikawa, Tomohisa Hasunuma, ISHII JUN, Yoshihiko Yasohara, Akihiko Kondo
    The Symposium on Biorefinery and Biprocess Topics, 2018 (iBio-N 2018), Nov. 2018, English, Nanjing, China, International conference
    Poster presentation

  • 次世代型微生物育種に資するスマートセル創出プラットフォームの開発
    蓮沼 誠久
    BioJapan 2018, Oct. 2018, Japanese, パシフィコ横浜, Domestic conference
    [Invited]
    Invited oral presentation

  • M2p3 Non-conventional酵母 Scheffersomyces stipitisを用いたレスベラトロール生産プロセスの開発
    小林優真, 猪熊健太郎, 蓮沼誠久, 近藤昭彦
    日本農芸化学会 関西・中部支部 2019年度合同神戸大会, Sep. 2018, Japanese, Domestic conference
    Oral presentation

  • 酵母の代謝解析の最新知見と化粧品原料への応用
    蓮沼 誠久
    第70回日本生物工学会大会 SK-IIランチョンセミナー, Sep. 2018, Japanese, Domestic conference
    Public discourse

  • 先端バイオ工学研究センターについて
    蓮沼 誠久
    iBioK第8回合成生物工学シンポジウム, Sep. 2018, Japanese, Domestic conference
    Nominated symposium

  • 二次代謝系の動的特性解析における大過剰化合物測定の有無の影響の数理解析
    富永 大輔, 川口 秀夫, 堀 良美, 蓮沼 誠久, 荻野 千秋, 油谷 幸代
    第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会, 関西大学 千里山キャンパス, Domestic conference
    Poster presentation

  • 鉄硫黄タンパク質高活性化に基づくキシロースからの1,2,4-ブタントリオール生産技術の開発
    湯川 貴弘, 番場 崇弘, 蓮沼 誠久, 近藤 昭彦
    第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会, 関西大学 千里山キャンパス, Domestic conference
    Poster presentation

  • 組換え出芽酵母Saccharomyces cerevisiaeによるキシロースからのグルタチオン生産
    小林 淳平, 佐々木 大介, 番場 崇弘, 蓮沼 誠久, 近藤 昭彦
    第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference
    Poster presentation

  • 接合型変換を利用したセルラーゼ群表層提示酵母の糖化活性及び発酵阻害物耐性向上
    北田 雄基, 猪熊 健太郎, 蓮沼 誠久, 近藤 昭彦
    第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会, 関西大学 千里山キャンパス, Domestic conference
    Poster presentation

  • 酵母Scheffersomyces stipitisを用いたレスベラトロール生産プロセスの開発
    小林 優真, 猪熊 健太郎, 蓮沼 誠久, 近藤 昭彦
    第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会, 関西大学 千里山キャンパス, Domestic conference
    Poster presentation

  • 酵母Scheffersomyces stipitisを宿主とした新規細胞表層提示システムの開発
    猪熊 健太郎, 小林 優真, 蓮沼 誠久, 近藤 昭彦
    第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会, 関西大学 千里山キャンパス, Domestic conference
    Poster presentation

  • 遺伝子組換え効率向上に向けたDNAリガーゼIV欠損Pichia pastoris株の開発
    ITO YOICHIRO, 渡邉 徹, 藍川 晋平, 西 輝之, 西山 陶三, NAKAMURA YASUYUKI, HASUNUMA TOMOHISA, 八十原 良彦, ISHII JUN, KONDO AKIHIKO
    第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会, 関西大学 千里山キャンパス, Domestic conference
    Oral presentation

  • ラン藻窒素代謝メカニズムの解明と物質生産への応用
    見市 絢香, 蓮沼 誠久, 近藤 昭彦
    第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference
    Poster presentation

  • スマートセル創出プラットフォームに資するメタボローム解析技術の開発
    蓮沼 誠久
    第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference
    Oral presentation

  • Pichia pastorisにおけるセントロメアDNA配列を用いた自律複製型プラスミドベクターの開発
    NAKAMURA YASUYUKI, 西 輝之, 野口 理紗, ITO YOICHIRO, 渡邉 徹, 西山 陶三, 藍川 晋平, HASUNUMA TOMOHISA, ISHII JUN, 八十原 良彦, KONDO AKIHIKO
    第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会, 関西大学 千里山キャンパス, Domestic conference
    Oral presentation

  • Temperature dependent succinate biosynthesis concurrent with alteration in central metabolism turnover in synechocystis SP.PCC6803
    TOMOHISA Hasunuma
    The International Symposium on Phototrophic Prokaryotes (ISPP), Aug. 2018, English, the Life Sciences Centre, The University of Buritish Columbia, International conference
    Oral presentation

  • 動的メタボロミクスの開発とスマートセルインダストリーへの展開
    蓮沼 誠久
    第13回 アジレントメタボロミクスセミナー, Jul. 2018, Japanese, 慶応義塾大学 三田キャンパス, Domestic conference
    [Invited]
    Invited oral presentation

  • スマートセル創出プラットフォームに資するハイスループット微生物構築・評価技術の開発
    蓮沼 誠久
    第20回 新産業技術促進検討会, Jul. 2018, Japanese, TKPガーデンシティPREMIUM神保町 プレミアムボールルーム, Domestic conference
    Oral presentation

  • Dynamic Metabolomics of Crabtree-Negative Yeast Kluyveromyces Marxianus
    TOMOHISA Hasunuma
    Metabolic Engineering 12, Jun. 2018, English, The Westin Grand Munich, International conference
    Oral presentation

  • Converting Chlamydomonas sp. JSC4 lipids to biodiesel via biocatalysis with Fusarium heterosporum lipase-expressing Aspergillus oryzae whole-cell
    JEROME Amoah, SHIH-HSIN. Ho, SHINJI Hama, AYUMI Yoshida, AKIHITO Nakanishi, TOMOHISA Hasunuma, CHIAKI Ogino, AKIHIKO Kondo
    The 8th International Conference on Algal Biomass, Biofuels and Bioproducts (AlgalBBB 2018), Jun. 2018, English, Motif Seattle, International conference
    Poster presentation

  • スマートセルインダストリーの創出に資する微生物育種プラットフォームの開発
    蓮沼 誠久
    ライフサイエンス技術部会・反応分科会講演会講演会「スマートセルインダストリーに関する研究開発動向」, Feb. 2018, Japanese, Domestic conference
    [Invited]
    Public discourse

  • Enhanced protein secretion by accumulation of novel effective factors in Pichia pastoris
    ITO YOICHIRO, NAKAMURA YASUYUKI, Shimpei Aikawa, Tomohisa Hasunuma, ISHII JUN, Akihiko Kondo
    The 9th International Symposium of Innovative BioProduction Kobe (iBioK), Feb. 2018, English, Kobe, Japan, International conference
    Poster presentation

  • A new autonomous replicating plasmid vector containing Pichia pastoris centromeric DNA
    NAKAMURA YASUYUKI, Teruyuki Nishi, Risa Noguchi, ITO YOICHIRO, Toru Watanabe, Tozo Nishiyama, Shimpei Aikawa, Tomohisa Hasunuma, ISHII JUN, Yuji Okubo, Akihiko Kondo
    The 9th International Symposium of Innovative BioProduction Kobe (iBioK), Feb. 2018, English, Kobe, Japan, International conference
    Poster presentation

  • 藻類オイル生産の実用化に向けた代謝メカニズム解析の重要性
    蓮沼 誠久
    シンポジウム「微細藻類を利用したバイオ燃料生産の研究開発動向」, Jan. 2018, Japanese, オフィス東京, Domestic conference
    Oral presentation

  • Efficient Xylitol Production from Kraft Pulp by a Cell Surface Engineered Strain ofSaccharomyces cerevisiae
    GUIRIMAND GEORGES, YVES GREGORY, Kentaro Inokuma, Takahiro Bamba, Kengo Sasaki, Chiaki Ogino, Tomohisa Hasunuma, Akihiko Kondo
    5th Asian Conference on Biomass Science, Jan. 2018, English, 日本エネルギー学会, 仙台市, International conference
    Poster presentation

  • Engineering Biologyによるバイオリファイナリーの構築とスマートセルインダストリーへの展開
    蓮沼 誠久
    静岡大学グリーン科学研究所 第4回シンポジウム, Nov. 2017, Japanese, Domestic conference
    [Invited]
    Invited oral presentation

  • 動的メタボロミクスの開発とスマートセルインダストリーへの展開
    蓮沼 誠久
    第11回メタボロームシンポジウム, Nov. 2017, Japanese, ホテル阪急エキスポパーク, Domestic conference
    Oral presentation

  • Development of dynamic metabolomics and its application to metabolic engineering
    TOMOHISA Hasunuma
    The 8th Kobe University Brussels European Centre Symposium, Nov. 2017, English, Vrije Universiteit Brussel U-Residence, International conference
    Oral presentation

  • スマートセルを創出する合成バイオプラットフォームの開発
    蓮沼 誠久
    BioJapan 2017, Oct. 2017, Japanese, パシフィコ横浜, Domestic conference
    Oral presentation

  • 油脂高蓄積クラミドモナス株の選抜育種によるバイオ燃料生産「明暗周期問題」の克服
    KATO YUICHI, 藤原 悠右, 小山 智己, 張 嘉修, Tomohisa Hasunuma, Akihiko Kondo
    第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference
    Oral presentation

  • 代謝工学的手法による海洋性ラン藻でのアスタキサンチン生産
    高木 綾湖, 蓮沼 誠久
    第31回カロテノイド研究談話会, Sep. 2017, Japanese, 京都薬科大学, Domestic conference
    Poster presentation

  • 代謝工学による黄色天然色素ルテイン高蓄積クラミドモナス株の創出
    阪口 健太, KATO YUICHI, Tomohisa Hasunuma, Akihiko Kondo
    第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference
    Poster presentation

  • アスタキサンチン生産能を付与した高増殖性ラン藻のトランスクリプトミクスおよび動的メタボロ ミクス
    高木 綾湖, Tomohisa Hasunuma, Akihiko Kondo
    第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference
    Poster presentation

  • Pichia pastorisのタンパク質分泌生産における新規有用因子の獲得とその蓄積による効果の検証
    Yoichiro Ito, Yasuyuki Nakamura, 西 輝之, 藍川 晋平, Tomohisa Hasunuma, Jun Ishii, Akihiko Kondo
    第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 早稲田大学 西早稲田キャンパス, Domestic conference
    Poster presentation

  • Pichia pastorisにおけるセントロメア配列を含む自律複製型プラスミドの開発
    Yasuyuki Nakamura, 西 輝之, 野口 理紗, Yoichiro Ito, 渡邉 徹, 西山 陶三, 藍川 晋平, Tomohisa Hasunuma, Jun Ishii, 八十原 良彦, Akihiko Kondo
    第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 早稲田大学 西早稲田キャンパス, Domestic conference
    Poster presentation

  • N-アセチルグルコサミン資化性酵母Scheffersomyces stipitisの基質別代謝特性の解析
    Kentaro Inokuma, Tomohisa Hasunuma, Akihiko Kondo
    第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference
    Oral presentation

  • Enhanced cell surface engineering of Saccharomyces cerevisiae and optimization of the fermentation process for efficient xylitol production from kraft pulp
    GUIRIMAND GEORGES, YVES GREGORY, Kentaro Inokuma, Takahiro Bamba, Kengo Sasaki, Chiaki Ogino, Tomohisa Hasunuma, Akihiko Kondo
    第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference
    Oral presentation

  • Challenge to development of “smart” microorganisms based on information analysis and its application to production of highly functional compounds
    TOMOHISA Hasunuma
    第69回日本生物工学会大会 国際シンポジウム, Sep. 2017, English, 早稲田大学 西早稲田キャンパス, International conference
    Oral presentation

  • スマートセルを創出する合成バイオプラットフォームの開発と応用への挑戦
    蓮沼 誠久
    合成生物工学シンポジウム, Aug. 2017, Japanese, 神戸大学百年記念館六甲ホール, Domestic conference
    Oral presentation

  • Enhancement in central metabolism and carotenoid biosynthesis by photosynthetic astaxanthin production in the recombinant marine cyanobacterium
    TOMOHISA Hasunuma
    The 18th International Symposium on Carotenoids 2017, Jul. 2017, English, The Lucerne Hall, International conference
    Oral presentation

  • Cell surface engineering of Saccharomyces cerevisiae for biomass breakdown
    TOMOHISA Hasunuma
    SB7.0 The Seventh International Meeting on Synethetic Biology, Jun. 2017, English, University Cultural Center, International conference
    Oral presentation

  • 進化した細胞表層工学によるバイオマス変換プロセスの開発と機能性物質生産への新展開
    蓮沼 誠久
    エネルギー・資源技術部会 バイオマス分科会 講演会「バイオマス資源変換触媒の研究動向」, Apr. 2017, Japanese, Domestic conference
    [Invited]
    Invited oral presentation

  • 変異チオール酸化酵素による酵母の酸化型グルタチオン 生産
    小林淳平, Sasaki Daisuke, 原 清敬, Hasunuma Tomohisa, KONDO Akihiko
    日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都市, Domestic conference
    Oral presentation

  • 代謝工学によるアスタキサンチン産生海洋性ラン藻の 開発と代謝メカニズムの解明
    高木綾湖, Hasunuma Tomohisa, KONDO Akihiko
    日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都市, Domestic conference
    Oral presentation

  • バイオ燃料生産に向けた耐塩性緑藻の進化工学的育種と 塩ストレス耐性による油脂生産低下メカニズムの解明
    KATO YUICHI, 賀 詩欣, 張 嘉修, 蓮沼 誠久, 近藤 昭彦
    日本農芸化学会2017年度大会, Mar. 2017, Japanese, 京都女子大学, Domestic conference
    Oral presentation

  • バイオ燃料生産に向けた耐塩性緑藻の進化工学的育種と 塩ストレス耐性による油脂生産低下メカニズムの解明
    加藤悠一, 賀 詩欣, 張 嘉修, Hasunuma Tomohisa, KONDO Akihiko
    日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都市, Domestic conference
    Oral presentation

  • ゲノム編集による Kluyveromyces marxianus の自在な 遺伝子改変
    南部-西田由美子, 西田敬二, 蓮沼 誠久, 近藤 昭彦
    日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都女子大学, Domestic conference
    Oral presentation

  • Saccharomyces cerevisiae による1,2,4-ブタントリオー ル生産技術の開発
    番場崇弘, Hasunuma Tomohisa, KONDO Akihiko
    日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都市, Domestic conference
    Oral presentation

  • 統合型バイオエタノール生産プロセスの構築に資する改良型酵 母細胞表層提示用遺伝子カセットの開発
    InokumaKentarou, Hasunuma Tomohisa, KONDO Akihiko
    第12回バイオマス科学会議, Jan. 2017, Japanese, 日本エネルギー学会, 東京都文京区, Domestic conference
    Oral presentation

  • バイオリファイナリーの構築に資する微生物細胞工場の創製
    蓮沼 誠久
    第21回関西大学先端科学技術シンポジウム, Jan. 2017, Japanese, 関西大学 千里山キャンパス, Domestic conference
    [Invited]
    Invited oral presentation

  • 日本独自の超高速微生物育種プラットフォーム「スマートセル・ファウンドリ」の開発
    蓮沼 誠久
    「植物等の生物を用いた高機能品生産技術の開発」キックオフシンポジウム, Nov. 2016, Japanese, ベイサイドホテル アジュール竹芝, Domestic conference
    Oral presentation

  • Development of dynamic metabolic profiling and its application to fuel and chemical production in algae
    TOMOHISA Hasunuma
    The 9th Asia-Pacific Conference on Algal Biotechnology (APCAB), Nov. 2016, English, Century Park Hotel, International conference
    Oral presentation

  • 藍藻 Synechocystis sp. PCC 6803 hox遺伝子変異株のコントロールされた培養条件における有機酸 生産
    中島 満晴, 飯嶋 寛子, 白井 智量, 岡本 真美, Hasunuma Tomohisa, KONDO Akihiko, 平井 優美, 小山内 崇
    第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference
    Oral presentation

  • 明暗周期下で効率的に油脂を生産するための海洋性緑藻培養技術の開発
    藤原 悠右, 加藤 悠一, 賀 詩欣, Hasunuma Tomohisa, KONDO Akihiko
    第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference
    Oral presentation

  • 酵母表層への酵素立体的配置技術の開発
    黒野 浩幹, InokumaKentarou, Hasunuma Tomohisa, KONDO Akihiko
    第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference
    Oral presentation

  • ラン藻スピルリナからの高生産エタノール変換プロセスの開発
    Aikawa Shimpei, InokumaKentarou, Wakai Satoshi, Sasaki Kengo, Hasunuma Tomohisa, KONDO Akihiko
    第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference
    Oral presentation

  • バイオ医薬生産に向けたCHO細胞培養情報データ解析システムの構築
    許 漢修, 荒木 通啓, Hasunuma Tomohisa, 河野 愛子, KONDO Akihiko
    第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference
    Oral presentation

  • バイオリファイナリーの構築に資する微生物細胞工場の創生
    蓮沼 誠久
    第118回触媒討論会, Sep. 2016, Japanese, 岩手大学, Domestic conference
    [Invited]
    Invited oral presentation

  • N-アセチルグルコサミン資化性酵母Scheffersomyces stipitisの特性解析
    InokumaKentarou, Hasunuma Tomohisa, KONDO Akihiko
    第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference
    Oral presentation

  • Enhanced protein secretion by accumulation of novel effective factors in Pichia pastoris
    Yoichiro Ito, Yasuyuki Nakamura, Aikawa Shimpei, Hasunuma Tomohisa, Ishii Jun, KONDO Akihiko
    The 14th International Congress on Yeasts (ICY2016), Sep. 2016, English, ICY14組織委員会, 淡路市, International conference
    Poster presentation

  • Bio-based fuel and chemicalproduction based on dynamic metabolic profiling of cynobacteria and microalgae
    TOMOHISA Hasunuma
    The 4th Asia-Oceania Algae Innovation Summit (AOAIS2016), Sep. 2016, English, East Lake International Conference Center, Wuhan, China, International conference
    Oral presentation

  • A new autonomous replicating plasmid vector containing centromere DNA sequence of Pichia pastoris
    NAKAMURA YASUYUKI, Teruyuki Nishi, Risa Noguchi, ITO YOICHIRO, Toru Watanabe, Tozo Nishiyama, Shimpei Aikawa, Tomohisa Hasunuma, ISHII JUN, Yoshihiko Yasohara, Akihiko Kondo
    The 14th International Congress on Yeasts (ICY2016), Sep. 2016, English, Awaji, Japan, International conference
    Poster presentation

  • 海洋性ラン藻Synechococcus sp. PCC 7002を用いたアスタキサンチン生産技術の開発
    高木綾湖, HASUNUMA TOMOHISA, KONDO Akihiko
    生物工学若手研究者の集い夏のセミナー2016, Jul. 2016, Japanese, 生物工学会, 府中市, Domestic conference
    Poster presentation

  • Development of cell factories of microalgae and cyanobacteria based on metabolic analysis
    Tomohisa Hasunuma
    KMB2016 Annual meeting and international symposium, Jun. 2016, English, International conference
    [Invited]
    Invited oral presentation

  • 代謝プロファイリング法の紹介と微生物育種技術への応用
    HASUNUMA TOMOHISA
    環境バイオテクノロジー学会2016年度大会, Jun. 2016, Japanese, サテライトキャンパスひろしま, Domestic conference
    Oral presentation

  • Synthetic Promoter Systems for Controlling Saccharomyces Cerevisiae Gene Expression
    Robert Sidney Cox III, Yumiko Nambu, Hasunuma Tomohisa, KONDO Akihiko
    Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conference
    Oral presentation

  • Engineering of a Novel Cellulose-Adherent Cellulolytic Saccharomyces Cerevisiae for Cellulosic Biofuel Production
    Zhuo Liu, Inokuma Kentarou, Hasunuma Tomohisa, KONDO Akihiko
    Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conference
    Poster presentation

  • Disruption of PHO13 Improves Ethanol Production Via the Xylose Isomerase Pathway
    Takahiro Bamba, Hasunuma Tomohisa, KONDO Akihiko
    Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conference
    Oral presentation

  • Direct and High-Productive Conversion from a Cyanobacterium Arthrospira Platensis to Ethanol
    Aikawa Shinpei, Inokuma Kentarou, Hasunuma Tomohisa, KONDO Akihiko
    Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conference
    Oral presentation

  • Alternative Inverse Metabolic Engineering Based on Transient Acclimation of Yeast Improves Acid-Containing Xylose Fermentation and Tolerance to Formic and Acetic Acids
    Hasunuma Tomohisa, KONDO Akihiko
    Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conference
    Oral presentation

  • 動的代謝プロファイリング技術の開発とバイオリファイナリーへの展開
    蓮沼 誠久
    第340回細胞工学研究会後援会／第220回遺伝子機能解析部門セミナー, Mar. 2016, Japanese, Domestic conference
    [Invited]
    Invited oral presentation

  • AFM力計測を用いた細胞表層提示酵素の特異的検出とマッピング評価
    Ogino Chiaki, Takenaka Musashi, Inokuma Kentarou, Hasunuma Tomohisa, Kondo Akihiko
    化学工学会第81年会, Mar. 2016, Japanese, Domestic conference
    Oral presentation

  • AFM力学計測法を利用した細胞表層空間に提示した酵素検出
    Takenaka Musashi, Ogino Chiaki, Kondo Akihiko, Inokuma Kentarou, Hasunuma Tomohisa, Kobayashi Takuya
    日本化学会第96春季年回, Mar. 2016, Japanese, Domestic conference
    Oral presentation

  • Dynamic metabolic analysis of microorganisms for the development of bio-based chemical production
    Tomohisa Hasunuma
    the 7th International Symposium of Innovative BioProduction Kobe (iBioK), Jan. 2016, English, International conference
    Invited oral presentation

  • 藻類を利用したバイオエタノール、バイオベース化学品生産
    蓮沼 誠久
    平成27 年度新資源生物変換研究会 シンポジウム, Jan. 2016, Japanese, バイオインダストリー協会, Domestic conference
    Oral presentation

  • リグノセルロース系バイオマスの同時糖化発酵によるフェニル乳酸生産
    Kawaguchi hideo, Teramura Hiroshi, Nakamura Satoko, Ogino Chiaki, Hara Kiyotaka, Hasunuma Tomohisa, Rounuma Kenichi, Takatani Naoki, Hirano Wataru, Sazuka Takashi, Kitano Hideki, Kondo Akihiko
    第11回バイオマス科学会議, Jan. 2016, Japanese, International conference
    Oral presentation

  • Live cell imaging and membrane protein mapping with atomic force microscope
    Musashi Takenaka, T. Kobayashi, Yusuke Miyachi, Kentarou Inokuma, Jun Ishii, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo
    The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015), Dec. 2015, English, International conference
    Oral presentation

  • Dynamic metabolic profiling of the marine microalga Chlamydomonas sp. JSC4 and enhancing its oil production
    Hasunuma Tomohisa, Kondo Akihiko
    Pacifichem 2015, Dec. 2015, English, International conference
    Oral presentation

  • Development of cell factories of microalgae and cyanobacteria based on metabolic analysis
    Tomohisa Hasunuma
    New Horizon in Biotechnology 2015, Nov. 2015, English, International conference
    [Invited]
    Invited oral presentation

  • ラン藻Arthrospira platenisからの高収率エタノール生産プロセスの開発
    Aikawa Shinpei, Inokuma Kentarou, Wakai, Sasaki Kengo, Hasunuma Tomohisa, Kondou Akihiko
    藍藻の分子生物学2015, Nov. 2015, Japanese, Domestic conference
    Oral presentation

  • 好機・低濃度グルコース条件下におけるSaccharomyces cerevisiaeの転写解析
    Sakihama Yuri, Hasunuma Tomohisa, Kondou Akihiko
    第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, Japanese, Domestic conference
    Oral presentation

  • キチン系基室からのバイオ燃料生産の検討
    Inokuma Kentarou, Hasunuma Tomohisa, Kondou Akihiko
    第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, Japanese, Domestic conference
    Oral presentation

  • Saccharomyces cerevisiae の糖代謝における転写制御ネットワークの予測
    Nanbu Yumiko, Sakihama Yuri, Araki, Hasunuma Tomohisa, Kondou Akihiko
    第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, Japanese, Domestic conference
    Oral presentation

  • Effect of PHO13 gene disruption on xylose productivity of yeast harboring xylose isomerase gene
    Bamba Takahiro, Hasunuma Tomohisa, Kondou Akihiko
    The 21th Symposium of Yong Asian Biochemical engineer's Communyty, Oct. 2015, English, International conference
    Oral presentation

  • Development of microbial cell factories for biorefinery
    Hasunuma Tomohisa, Kondou Akihio
    第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, English, Domestic conference
    Oral presentation

  • Conversion of high phospholipid-containing oils to biodiesel using immobilizes Aspergillus oryzae whole cell biocatalyst ezpressing Fusarium heteroporum lipase
    Jerom Amoah, shin-Hsin Ho, Hama Shinji, Yoshida Ayumi, Nakanishi Akihito, Hasunuma Tomohisa, Ogino Chiaki, Kondo Akihiko
    The 21th Symposium of Yong Asian Biochemical engineer's Communyty, Oct. 2015, English, International conference
    Oral presentation

  • Combined cell-surface display-and secretion-based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae
    Zuo Liu, Inokuma Kentaro, Hasunuma Tomohisa, Kondo Akihiko
    第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, Japanese, Domestic conference
    Oral presentation

  • 藻類のエネルギー生産における物質代謝メカニズムの解析
    蓮沼 誠久
    日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference
    Oral presentation

  • メタボロームのターンオーバー解析技術（代謝プロファイリング）の開発と物質生産への応用
    蓮沼 誠久
    iBioK第5回 合成生物工学シンポジウム, Aug. 2015, Japanese, Domestic conference
    [Invited]
    Nominated symposium

  • マルチオミクス解析に基づく合理的細胞育種技術の開発
    蓮沼 誠久
    HMTセミナー 物質生産におけるメタボロミクスのいま, Jul. 2015, Japanese, Domestic conference
    [Invited]
    Public discourse

  • Dynamic metabolic profiling of cyanobacteria for bio-based chemical production
    TOMOHISA Hasunuma
    11th Annual International Conference of the Metabolomics Society, Jun. 2015, English, Hyatt Regency Burlingame, International conference
    Oral presentation

  • Biofuel Production from Microalgae and Cyanobacteria Based on Metabolic Profiling
    TOMOHISA Hasunuma
    KMB2015, 42nd Annual Meeting & International Symposium, Jun. 2015, English, HICO, Gyeongju, Korea, International conference
    Oral presentation

  • Development of microbial cell factories based on metabolic engineering
    Tomohisa Hasunuma
    2015 KSBB Spring Meeting and International Symposium, Apr. 2015, English, International conference
    [Invited]
    Invited oral presentation

  • 産業用酵母のゲノムシャッフリングによる高温及び酸耐性の付与と高効率キシロース発酵
    岩本 遼, 猪熊 健太郎, HASUNUMA TOMOHISA, KONDO AKIHIKO
    日本農芸化学会2015年度大会, Mar. 2015, Japanese, 日本農芸化学会, 岡山市, Domestic conference
    Oral presentation

  • アミラーゼ表層提示酵母によるシアノバクテリアArthrospira platensisからの高濃度エタノール生産
    藍川 晋平, 猪熊 健太郎, SASAKI KENGO, HASUNUMA TOMOHISA
    日本農芸化学会2015年度大会, Mar. 2015, Japanese, 日本農芸化学会, 岡山市, Domestic conference
    Oral presentation

  • 合成生物工学によるバイオ燃料生産に資する微生物の創製
    蓮沼 誠久
    広島大学バイオマスイブニングセミナー, Dec. 2014, Japanese, Domestic conference
    [Invited]
    Public discourse

  • 代謝システム解析に基づく細胞育種とバイオ医薬品生産への応用
    蓮沼 誠久
    第30回バイオロジカルズ（タンパク医薬）製造技術研究会セミナー, Nov. 2014, Japanese, Domestic conference
    [Invited]
    Public discourse

  • 代謝システム解析に基づく合理的細胞育種技術の開発
    蓮沼 誠久
    iBioK 第4回合成生物工学シンポジウム, Nov. 2014, Japanese, Domestic conference
    Nominated symposium

  • 微細藻類の代謝系解析に基づく次世代バイオ燃料生産への挑戦
    蓮沼 誠久
    バイオマス研究会, Nov. 2014, Japanese, Domestic conference
    [Invited]
    Public discourse

  • The development of bioconversion process to bio-ethanol from cyanobacteria Spirulina biomass
    AIKAWA Shinpei, HASUNUMA Tomohisa, KONDO Akihiko
    5th International Symposium on Innovative Bioprocess in Tainan(i-BioT2014), Nov. 2014, English, National Cheng Kung University, Tainan, China, International conference
    Oral presentation

  • Development of the novel yeast cell-surface display system for consolidated bioethanol production from biomass resources
    INOKUMA Kentaro, HASUNUMA Tomohisa, KONDO Akihiko
    5th International Symposium on Innovative Bioprocess in Tainan(i-BioT2014), Nov. 2014, English, National Cheng Kung University, Tainan, China, International conference
    Oral presentation

  • Development of Dynamic Metabolic Profiling of Cyanobacteria and Microalgae
    HASUNUMA Tomohisa, KONDO Akihiko
    3rd Asia-Oceania Algae Innovation Summit 2014, Nov. 2014, English, AOAIS2014, 済州、韓国, International conference
    Oral presentation

  • Development of dynamic metabolic profiling of cyanobacteria and microalgae
    HASUNUMA Tomohisa
    5th International Symposium on Innovative Bioprocess in Tainan(i-BioT2014), Nov. 2014, English, National Cheng Kung University, Tainan, China, International conference
    Oral presentation

  • オミクス解析のバイオリファイナリー
    蓮沼 誠久
    アジレント・メタボロミクスデイズ2014, Oct. 2014, Japanese, Domestic conference
    [Invited]
    Public discourse

  • Biodiesel production in marine microalgae through metabolic profiling and an innovative salinity-gradient strategy
    Tomohisa Hasunuma
    Korean Marine Biology and Biotechnology International Symposium, Oct. 2014, English, International conference
    [Invited]
    Invited oral presentation

  • 代謝系解析に基づくラン藻・微細藻からのバイオ燃料生産への挑戦
    HASUNUMA TOMOHISA
    日本植物学会第78回大会, Sep. 2014, Japanese, 日本植物学会, 川崎市, Domestic conference
    Oral presentation

  • 代謝プロファイリングに基づく微生物育種技術の開発と応用
    HASUNUMA TOMOHISA
    第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference
    Poster presentation

  • 酵母によるタンパクの高効率細胞表層提示ならびに分泌生産のための新規分泌シグナル配列の検討
    猪熊 健太郎, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference
    Poster presentation

  • 海産性緑藻 Chlamydomonas sp. JSC4 の細胞組成評価と代謝解析に基づいた油脂高生産系の開発
    中西 昭仁, 賀 詩欣, 張 嘉修, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference
    Poster presentation

  • 塩ストレス下での海洋性シアノバクテリアの代謝プロファイリング
    西田 篤実, 藍川 晋平, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference
    Poster presentation

  • バイオ医薬開発に有用なCHO 細胞の改良、培養状態解明につながるデータの構築
    伊賀 朋世, HASUNUMA TOMOHISA, ARAKI MICHIHIRO, KONDO AKIHIKO
    第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference
    Poster presentation

  • バイオリファイナリーに向けた微生物の開発と代謝系解析の応用
    HASUNUMA TOMOHISA
    日本植物学会第78回大会ランチョンセミナー, Sep. 2014, Japanese, 島津製作所, 川崎市, Domestic conference
    [Invited]
    Invited oral presentation

  • バイオマス糖化液由来成分が大腸菌のフェニル乳酸発酵に与える影響
    KAWAGUCHI HIDEO, 寺村 浩, 中村 聡子, OGINO CHIAKI, HARA KIYOTAKA, HASUNUMA TOMOHISA, 老沼 研一, 高谷 直樹, 平野 恒, 佐塚 隆志, 北野 英己, KONDO AKIHIKO
    第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference
    Poster presentation

  • コリネ菌におけるフルフラールの分解
    TSUGE YOTA, 堀 良美, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference
    Poster presentation

  • キシロース資化性酵母における遺伝子発現プロファイルの経時変化解析
    南部 由美子, 崎濱 由梨, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference
    Poster presentation

  • アミラーゼ表層提示酵母によるラン藻スピルリナからの高濃度エタノール生産
    藍川 晋平, 猪熊 健太郎, SASAKI KENGO, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference
    Poster presentation

  • PHO13 遺伝子の欠損がキシロースイソメラーゼ導入酵母のエタノール生産へ与える影響
    番場 崇弘, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference
    Poster presentation

  • luyveromyces marxianus の好気条件での糖代謝系の解析
    崎濱 由梨, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference
    Poster presentation

  • Effect of illumination coupled with nitrogen depletion on biodiesel production of a marine microalga Chlamydomonas sp. JSC4
    HO Shih-Hsin, NAKANISHI Akihito, YE Xiaoting, CHANG Jo-Shu, HARA Kiyotaka, KONDO Akihiko, HASUNUMA Tomohisa
    第66回日本生物工学会大会, Sep. 2014, English, 日本生物工学会, 札幌市, Domestic conference
    Poster presentation

  • 色素生産を指標としたCoA代謝経路の評価系
    磯貝 章太, ISHII JUN, HASUNUMA TOMOHISA, KONDO AKIHIKO
    生物工学若手研究者の集い夏のセミナー, Jul. 2014, Japanese, 生物工学会, 神戸市, Domestic conference
    Poster presentation

  • キシロースイソメラーゼ導入酵母におけるPHO13遺伝子欠損がエタノール生産へ与える影響
    番場 崇弘, HASUNUMA TOMOHISA, KONDO AKIHIKO
    生物工学若手研究者の集い夏のセミナー, Jul. 2014, Japanese, 生物工学会, 神戸市, Domestic conference
    Poster presentation

  • Dynamic Metabolic Profiling of Cyanobacteria Under Conditions of Nitrate Depletion
    HASUNUMA Tomohisa
    Metabolic Engineering X, Jun. 2014, English, Society for Biological Engineering, Vancouver, Canada, International conference
    Oral presentation

  • 発酵生産プロセスのための海洋性ラン藻の利用
    藍川 晋平, 西田 篤実, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第16回マリンバイオテクノロジー学会大会, May 2014, Japanese, マリンバイオテクノロジー学会, 津市, Domestic conference
    Poster presentation

  • 微細藻類によるバイオ燃料や化学物質製造
    KONDO AKIHIKO, HASUNUMA TOMOHISA
    日本化学会第94春季年会, Mar. 2014, Japanese, 日本化学会, 名古屋市, Domestic conference
    Oral presentation

  • 統合型リグノセルロース系エタノール生産プロセスに資するセルラーゼ表層提示酵母の開発
    猪熊 健太郎, 番場 崇弘, HASUNUMA TOMOHISA, KONDO AKIHIKO
    日本農芸化学会2014年度大会, Mar. 2014, Japanese, 日本農芸化学会, 川崎市, Domestic conference
    Oral presentation

  • 代謝工学的手法によるグリコーゲン高生産藍藻の創製
    曽根辻 諒彦, 藍川 晋平, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第16回化学工学会学生発表会(堺大会), Mar. 2014, Japanese, 化学工学会, 大阪市, Domestic conference
    Oral presentation

  • Development of lipid productive engineering with Chlamydomonas sp. Under marine condition
    HASUNUMA Tomohisa
    Asian Congress on Biotechnology - ACB 2013, Dec. 2013, English, Asian Federation of Biotechnolgy, New Delhi, India, International conference
    Poster presentation

  • ラン藻動的代謝プロファイリング技術の開発と応用
    HASUNUMA TOMOHISA
    ラン藻の分子生物学2013, Nov. 2013, Japanese, かずさDNA研究所, 木更津市, Domestic conference
    Oral presentation

  • Development of microalgal cell factories based on sysmtems biology approach
    Tomohisa Hasunuma
    1st Korea-Japan Microalgae Symposium, Oct. 2013, English, International conference
    [Invited]
    Invited oral presentation

  • Biofuel production from halophilic algae
    Tomohisa Hasunuma
    i-BioB 2013, Oct. 2013, English, International conference
    [Invited]
    Invited oral presentation

  • in vivo 13C標識法によるラン藻動的代謝プロファイリング
    蓮沼 誠久
    第28回つくば藻類・プロティストフォーラム, Oct. 2013, Japanese, Domestic conference
    [Invited]
    Nominated symposium

  • 新たな挑戦:イノベーティブ・バイオプロダクション
    蓮沼 誠久, 近藤 昭彦
    INCHEM TOKYO 2013 産学官マッチングフォーラム, Oct. 2013, Japanese, Domestic conference
    [Invited]
    Nominated symposium

  • 緑藻Chlamydomonas orbicularisを用いた海水塩存在下での油脂高生産条件の開発
    中西 昭仁, 賀 詩欣, 藍川 晋, 張 嘉修, KONDO AKIHIKO, HASUNUMA TOMOHISA
    第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference
    Poster presentation

  • 統合型リグノセルロース系エタノール生産プロセスに資する新規酵母細胞表層提示システムの開発
    猪熊 健太郎, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference
    Poster presentation

  • 統合バイオプロセスによる環境調和型セルロース系エタノール生産に資する前処理技術の開発
    崎濱 由梨, 俣野 結城, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference
    Poster presentation

  • 海洋性シアノバクテリアによるバイオリファイナリーのためのグリコーゲン生産
    西田 篤実, 藍川 晋平, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference
    Poster presentation

  • 海水中のイオン濃度が糖質源Spirulinaの増殖;光合成に与える影響
    藍川 晋平, 秋元 誠志, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference
    Poster presentation

  • 稲わら希硫酸処理液のナノフィルトレーションによる糖濃縮
    SASAKI KENGO, HASUNUMA TOMOHISA, OGINO CHIAKI, KONDO AKIHIKO
    第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference
    Poster presentation

  • キシロース資化性Saccharomyces cerevisiaeにおける代謝プログラム制御によるギ酸;酢酸耐性関連遺伝子の同定
    阪本 貴俊, 堀 良美, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference
    Poster presentation

  • キシロース資化性Saccharomyces cerevisiaeにおけるリン酸シグナル伝達系の制御によるキシロースからのエタノール生産の高効率化
    HASUNUMA TOMOHISA, KONDO AKIHIKO
    第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference
    Poster presentation

  • Phototrophic cultivation of a marine microalga Chlamydomonas orbicularis CO2 fixation and biodiesel production:Effect of medium composition, nitrogen deplection, and sea salt concentration
    HO Shih-Hsin, NAKANISHI Akihito, AIKAWA Shimpei, CHANG Jo-Shu, KONDO Akihiko, HASUNUMA Tomohisa
    第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference
    Poster presentation

  • メタボローム解析による次世代バイオ燃料生産のための微細藻代謝改変戦略の導出
    蓮沼 誠久
    微細藻類研究会2013, Jun. 2013, Japanese, Domestic conference
    [Invited]
    Public discourse

  • Direct conversion of cyanobacteria to ethanol without pretreatment or enzymatic hydrolysis process
    AIKAWA S, YAMADA R, YAMGISHI T, CHNG J.S, HASUNUMA Tomohisa, KONDO Akihiko
    The 3rd International Conference on Algal Biomass、Biofuels and Bioproducts, Jun. 2013, English, Elsevier, Toronto, Canada, International conference
    Poster presentation

  • シアノバクテリアからの直接バイオエタノール変換技術の開発
    藍川 晋平, 山田 亮祐, 和泉 自泰, 山岸 隆博, 松田 史生, 川井 浩史, Jo-shu Chang, HASUNUMA TOMOHISA, KONDO AKIHIKO
    第４回日本光合成学会年会, May 2013, Japanese, 日本光合成学会, 名古屋市, Domestic conference
    Poster presentation

  • Development of Microbial Cell Factories for Bio-refinery
    KONDO Akihiko, HASUNUMA Tomohisa
    Internatinal meeting of the Microbiorogical Society of Korea, May 2013, English, Microbiorogical Society of Korea, Jeonju, Korea, International conference
    Oral presentation

  • 微細藻バイオリファイナリーに資する動的代謝プロファイリング解析
    HASUNUMA TOMOHISA
    日本農芸化学会2013年度大会, Mar. 2013, Japanese, 日本農芸化学会, 仙台市, Domestic conference
    Oral presentation

  • 発酵阻害物存在下におけるキシロースからのエタノール発酵向上酵母の構築
    崎濱 由梨, HASUNUMA TOMOHISA, KONDO AKIHIKO
    日本農芸化学会2013年度大会, Mar. 2013, Japanese, 日本農芸化学会, 仙台市, Domestic conference
    Oral presentation

  • 耐塩性シアノバクテリアArthrospira platensisからの効率の良いグリコーゲン回収法の構築
    中西 昭仁, 藍川 晋平, HASUNUMA TOMOHISA, KONDO AKIHIKO
    日本農芸化学会2013年度大会, Mar. 2013, Japanese, 日本農芸化学会, 仙台市, Domestic conference
    Oral presentation

  • 神戸大学におけるバイオリファイナリーの進展
    HASUNUMA TOMOHISA, KONDO AKIHIKO
    化学工学会第78年会, Mar. 2013, Japanese, 化学工学会, 豊中市, Domestic conference
    Oral presentation

  • リグノセルロース系バイオマスからの統合型バイオエタノール生産に資する新規酵母細胞表層提示システムの開発
    猪熊 健太郎, HASUNUMA TOMOHISA, KONDO AKIHIKO
    日本農芸化学会2013年度大会, Mar. 2013, Japanese, 日本農芸化学会, 仙台市, Domestic conference
    Oral presentation

  • Biorefinery from microalgae through fermentation and metabolic engineering
    Tomohisa Hasunuma
    SBJ 64th Annual Meeting, Oct. 2012, English, International conference
    [Invited]
    Invited oral presentation

  • リパーゼ分泌挙動の異なる糸状菌Rhizopus oryzae のメタボローム解析
    吉田 あゆみ, 濵 真司, HASUNUMA TOMOHISA, 山本 博之, KONDO AKIHIKO
    第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference
    Oral presentation

  • イオン液体前処理とキシロース資化性酵母株を用いたバイオマス糖化発酵プロセスの効率化
    小倉 一真, 仲島 菜々実, YAMADA RYOSUKE, HASUNUMA TOMOHISA, 神谷 典穂, 石田 亘広, 齋藤 聡志, OGINO CHIAKI
    第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference
    Oral presentation

  • Development of sustainable biorefinery based on microalgae
    HASUNUMA Tomohisa
    5th International Conference on Industrial Bioprocesses (IFIB-2012), Oct. 2012, English, National Taiwan University of Science and Technology, Taiwan, China, International conference
    Oral presentation

  • Flux balance analysis of xylose-fermenting Saccharomyces cerevisiae strain
    KATO Hiroko, NAGATA Kento, HASUNUMA Tomohisa, MATSUDA Fumio, KONDO Akihiko
    The 15th International Biotechnology Symposium (IBS 2012), Sep. 2012, English, The Korean Society for Biotechnology and Bioengineering, Deague, Korea, International conference
    Oral presentation

  • Cell recycle batch fermentation of high‐solid lignocellulose using a recombinant cellulase‐displaying yeast strain for high yield ethanol production
    HASUNUMA Tomohisa, KONDO Akihiko
    The 13th International Congress on Yeasts, Aug. 2012, English, ICY 2012 Organizing Committee, Madison, USA, International conference
    Oral presentation

  • バイオリファイナリー構築に資する微生物細胞工場の創製
    HASUNUMA TOMOHISA
    アジレント・メタボロミクスセミナー2012, Jul. 2012, Japanese, アジレント・テクノロジー㈱, 豊中市, Domestic conference
    Oral presentation

  • Control of phosphate metabolism in a xylose-fermenting yeast strain improves ethanol production from xylose
    HASUNUMA Tomohisa
    Metabolic EngineeringⅨ, Jun. 2012, English, Engineering Conferences International, Biarritz, France, International conference
    Oral presentation

  • Microbial engineering for production of biofuels and biorefinery products
    Tomohisa Hasunuma
    4th A-IMBN Annual Conference, Mar. 2012, English, International conference
    [Invited]
    Invited oral presentation

  • シアノバクテリアArthrospira platensisを炭素源としたアミラーゼ発現酵母による直接エタノール生産
    藍川 晋平, 和泉 自泰, 山田 亮祐, 松田 史生, 蓮沼 誠久, 近藤 昭彦
    日本農芸化学会2012年度大会, Mar. 2012, Japanese, 日本農芸化学会, 京都市, Domestic conference
    Oral presentation

  • Metabolic pathway engineering in cyanobacteria Synechocystis sp.PCC 6803 for the production of lactate
    JOSEPH Ancy, AIKAWA Shimpei, HASUNUMA Tomohisa, MATSUDA Fumio, KONDO Akihiko
    日本農芸化学会2012年度大会, Mar. 2012, Japanese, 日本農芸化学会, 京都市, Domestic conference
    Oral presentation

  • イオン液体共存下における酵母のキシロース発酵能評価
    小倉 一真, 仲島 菜々実, 山田 亮祐, 蓮沼 誠久, 荻野 千秋, 近藤 昭彦
    第2回イオン液体討論会, Dec. 2011, Japanese, イオン液体研究会, 京都市, Domestic conference
    Oral presentation

  • Production of bio-ethanol from microalgae through cell surface engineering
    Tomohisa Hasunuma, Akihiko Kondo
    5th International Algae Congress 2011, Dec. 2011, English, DLG BENELUX, Berlin, Germany, International conference
    Oral presentation

  • 補助食品・スピルリナのメタボローム解析
    蓮沼 誠久
    LECOジャパン・食品分析セミナー, Nov. 2011, Japanese, Domestic conference
    [Invited]
    Public discourse

  • CE-TOFMSを用いたバイオエタノール生産酵母の代謝プロファイリング
    蓮沼 誠久
    第31回キャピラリー電気泳動シンポジウム, Nov. 2011, Japanese, 日本分析化学会電気泳動分析研究懇談会, 山形県鶴岡市, Domestic conference
    Oral presentation

  • 発酵阻害物耐性遺伝子を導入したキシロース資化性二倍体酵母によるリグノセルロース水熱分解液の繰返し発酵
    蓮沼 誠久, 近藤 昭彦
    第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference
    Oral presentation

  • 細胞内酸化還元バランス調整能を付与したキシロース資化性酵母の取得
    須賀 裕之, 蓮沼 誠久, 松田 史生, 近藤 昭彦
    第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference
    Oral presentation

  • 回転式発酵槽を用いた稲わら水熱処理物からのエタノール生産の効率化
    俣野 結城, 蓮沼 誠久, 近藤 昭彦
    第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference
    Oral presentation

  • ユーグレナによるバイオ燃料生産のための高密度CO2 固定サイクルの構築
    林 雅弘, 松田 史生, 蓮沼 誠久, 近藤 昭彦
    第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference
    Oral presentation

  • メタボロミクスによるリン酸代謝関連遺伝子欠損酵母の発酵特性の解析
    藤冨 桂介, 蓮沼 誠久, 近藤 昭彦
    第3回 神戸大学バイオサイエンス研究会・若手研究者交流会, Sep. 2011, Japanese, 神戸大学バイオサイエンス研究会・若手研究者交流会, 神戸市, Domestic conference
    Poster presentation

  • フラン化合物存在下でのXR-XDH 発現酵母を用いたキシロース発酵におけるNADH 依存性Adh1過剰発現のインパクト
    石井 純, 吉村 一也, 蓮沼 誠久, 近藤 昭彦
    第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference
    Oral presentation

  • キシロース資化性酵母Saccharomyces cerevisiae におけるPHO13 遺伝子欠損がエタノール生産に与える影響及び発酵阻害物耐性の評価
    藤冨 桂介, 蓮沼 誠久, 近藤 昭彦
    第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference
    Oral presentation

  • シンセティックバイオエンジニアリングによるバイオエタノール生産酵母の育種
    蓮沼 誠久
    第99回 醗酵学懇話会, Aug. 2011, Japanese, 日本生物工学会 関西支部, 神戸市, Domestic conference
    Invited oral presentation

  • Repeated batch-fermentation of rice straw hydrolysate to ethanol using metabolically engineered Saccharomyces cerevisiae strains
    HASUNUMA Tomohisa, KONDO Akihiko
    SIM2011 Annual Meeting and Exhibition, Jul. 2011, English, Society for Industrial Microbiology, New Orleans, USA, International conference
    Oral presentation

  • Development of microbial cell factories for biofuel production from biomass by conslidated bioprocessing
    KONDO Akihiko, HASUNUMA Tomohisa
    SIM2011 Annual Meeting and Exhibition, Jul. 2011, English, Society for Industrial Microbiology, New Orleans, USA, International conference
    Oral presentation

  • 合成生物工学によるバイオ燃料生産のための微生物細胞工場の創製
    蓮沼 誠久
    バイオマスの今、そして未来, Jun. 2011, Japanese, Domestic conference
    [Invited]
    Public discourse

  • Development of Microbial Cell Factories for Biofuel Production from Biomass
    Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo
    2011 International Conference for Bioeconomy, Jun. 2011, English, Tianjin Institute of Scientific and Technical Information, Tianjin、China, International conference
    Oral presentation

  • Cellulosic ethanol Production in Saccharomyces cerevisiae through Metabolic Engineering Based on Metabolomic Approach
    HASUNUMA Tomohisa
    Asian Congress on Biotechnology 2011, May 2011, English, ACB-2011Organizing Committee, Shanghai, China, International conference
    Oral presentation

  • セルラーゼ表層提示株を用いた稲わら水熱処理物からの エタノール生産プロセスの効率化
    俣野 結城, HASUNUMA Tomohisa, KONDO Akihiko
    日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference
    Oral presentation

  • セルラーゼを表層提示した高温耐性酵母Kluyveromyces marxianusを用いたセルロースからのエタノール生産
    HASUNUMA Tomohisa, 柳瀬 修平, YAMADA Ryosuke, KONDO Akihiko
    日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference
    Oral presentation

  • キシロース発酵形質転換酵母における代謝コントロールの解析
    MATSUDA Fumio, 加藤 寛子, HASUNUMA Tomohisa, KONDO Akihiko
    日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference
    Oral presentation

  • PHO13欠損株を用いたキシロースからのエタノール生産
    藤冨 桂介, 三田 智也, HASUNUMA Tomohisa, MATSUDA Fumio, KONDO Akihiko
    日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference
    Oral presentation

  • PHO13欠損株を用いたキシロースからのエタノール生産
    藤冨 桂介, 三田 智也, HASUNUMA Tomohisa, KONDO Akihiko
    化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference
    Oral presentation

  • Production of bio-ethanol from lignocellulosic materials through the engineering of yeast cell surface and metabolic pathway
    Tomohisa Hasunuma
    4th Japan-Korea Biomass Symposium, Nov. 2010, English, International conference
    [Invited]
    Invited oral presentation

  • 有用希少イノシトールのバイオアベイラビリティーとバイオコンバージョン生産
    YOSHIDA Ken-ichi, HASUNUMA Tomohisa
    第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference
    Oral presentation

  • 微細藻を利用したバイオ燃料生産システムの構築へ向けて
    KONDO Akihiko, HASUNUMA Tomohisa
    第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference
    Oral presentation

  • 発酵阻害物存在下におけるキシロースからの効率的なエタノール生産
    三田 智也, YAMADA Ryosuke, HASUNUMA Tomohisa, KONDO Akihiko
    第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference
    Poster presentation

  • 海洋性微細藻のシステムバイオロジー解析に向けての網羅的代謝プロファイリング技術の開発
    和泉 自泰, 藍川 晋平, MATSUDA Fumio, HASUNUMA Tomohisa, KONDO Akihiko
    第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference
    Poster presentation

  • ワイン酵母OC-2 トリプルマーカー株をプラットフォームとした高β – グルコシダーゼ活性と高キシロシダーゼ活性を持つキシロース発酵酵母の作製
    齋藤 聡志, TANAKA Tsutomu, HASUNUMA Tomohisa, KONDO Akihiko
    第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference
    Poster presentation

  • ラン藻Spirulina (Arthrospira) platensis のグリコーゲン蓄積量と増殖速度に対する光強度の影響
    藍川 晋平, 和泉 自泰, MATSUDA Fumio, HASUNUMA Tomohisa, KONDO Akihiko
    第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference
    Poster presentation

  • キシロース資化性形質転換酵母の代謝解析
    MATSUDA Fumio, 加藤 寛子, HASUNUMA Tomohisa, KONDO Akihiko
    第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference
    Poster presentation

  • キシロース資化性Saccharomyces cerevisiae におけるアルコールデヒドロゲナーゼ過剰発現およびフラン化合物存在下でのキシロース発酵特性
    吉村 一也, ISHII Jun, HASUNUMA Tomohisa, KONDO Akihiko
    第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference
    Poster presentation

  • メタボロームを利用した新規バイオエタノール生産酵母の育種
    HASUNUMA Tomohisa
    第5回メタボロームシンポジウム, Sep. 2010, Japanese, メタボロームシンポジウム実行委員会, 山形県鶴岡市, Domestic conference
    Oral presentation

  • Production of bio-ethanol from lignocellulosic materials through engineering yeast cell surface and metabolic pathway
    KONDO Akihiko, MATSUDA Fumio, HASUNUMA Tomohisa
    SIM 2010 Annual Meeting, Aug. 2010, English, Society for Industrial Microbiology, San Francisco, USA, International conference
    Oral presentation

  • Ethanol production from cellulosic material using the thermotolerant yeast displaying cellulolytic enzymes on the cell surface
    HASUNUMA Tomohisa, YANASE, S, YAMADA Ryosuke, KONDO Akihiko
    SIM 2010 Annual Meeting, Aug. 2010, English, Society for Industrial Microbiology, San Francisco, USA, International conference
    Oral presentation

  • Development of bioethanol production from marine microalgae
    HASUNUMA Tomohisa
    The 1st International Symposium on the Nitrogen Nutrition of Plants, Jul. 2010, English, Nitrogen 2010 Organizing Committee, 犬山市, International conference
    Poster presentation

  • Development of bioethanol production from marine microalgae
    HASUNUMA Tomohisa, AIKAWA, S, IZUMI, Y, MATSUDA Fumio, KONDO Akihiko
    2010 International Symposium on Advanced Biological Engineering, Jul. 2010, English, Biotechnology for Sustainable Industry and Society, Beijing, China, International conference
    Oral presentation

  • Ammonium assimilation in rice leaves: functions of the chloroplastic andcytosolic phosphoenolpyruvate carboxylases (PEPCs) in supplying carbonskeletons
    MIYAZAWA, S, MASUMOTO, C, HASUNUMA Tomohisa, MIYIAO, M
    The 1st International Symposium on the Nitrogen Nutrition of Plants, Jul. 2010, English, Nitrogen 2010 Organizing Committee, 犬山市, International conference
    Poster presentation

  • Metabolomic approach to identify molecules that are important for acetic acid tolerance in a recombinant xylose-fermenting yeast strain
    HASUNUMA Tomohisa
    Metabolic Engineering VIII: Metabolic Engineering for Green Growth, Jun. 2010, English, Engineering Conferences International, Jeju、Korea, International conference
    Oral presentation

  • Efficient Production of Cellulosic Ethanol in Saccharomyces cerevisiae through Metabolic Engineering Based on a Metabolomic Approach
    HASUNUMA Tomohisa
    Microbes at Work JSPS-SLU colloquium, Jun. 2010, English, JSPS-SLU, Uppsala, Sweden, International conference
    Oral presentation

  • Construction of consolidated bioprocesses for production of bio-fuels and chemicals by using cell surface engineered microbial cells
    KONDO Akihiko, HASUNUMA Tomohisa, OGINO Chiaki, TANAKA Tsutomu
    RENEWABLE ENERGY 2010, Jun. 2010, English, International Solar Energy Society, 横浜市, International conference
    Oral presentation

  • 酢酸耐性酵母によるキシロースからの効率的バイオエタノール生産
    三田 智也, 山田 亮祐, 蓮沼 誠久, 近藤 昭彦
    日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference
    Oral presentation

  • 細胞表層工学技術を用いたヘミセルロース系バイオマスからのエタノール生産
    阪本 貴俊, 蓮沼 誠久, 堀 良美, 山田 亮祐, 荻野 千秋, 近藤 昭彦
    日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference
    Oral presentation

  • バイオマスからの燃料・化学品生産を目指した細胞工場の創製
    近藤 昭彦, 荻野 千秋, 蓮沼 誠久, 田中 勉
    日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference
    Oral presentation

  • キシロース資化性遺伝子組換え酵母の代謝フラックス解析
    松田 史生, 三田 智也, 蓮沼 誠久, 近藤 昭彦
    日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference
    Oral presentation

  • D-アラビノースは酵母のエリスロアスコルビン酸の内在前駆体か
    尼子 克己, 森村 健一, 蓮沼 誠久, 近藤 昭彦, 合田 清
    日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference
    Oral presentation

  • A synthetic biology approach for efficient production of cellulosic ethanol in Saccharomyces cerevisiae
    Tomohisa Hasunuma
    The 2nd International Symposium of Innovative BioProduction Kobe, Jan. 2010, English, International conference
    Invited oral presentation

  • Effective biofuel production process from biomass reseources by whole-cell biocatalyst
    Tomohisa Hasunuma
    Airbus meeting, Nov. 2009, English, International conference
    [Invited]
    Invited oral presentation

  • アーミング酵母を用いた統合プロセスによるバイオエタノール生産
    蓮沼 誠久
    日本化学会関東支部講演会 「バイオマス変換最前線」, Nov. 2009, Japanese, Domestic conference
    [Invited]
    Invited oral presentation

  • Dynamic metabolic profiling in plant leaves using in vivo stable isotope labeling
    蓮沼 誠久, 近藤 昭彦, 福崎 英一郎
    APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference
    Oral presentation

  • Bioethaol fermentation from mixed sugar by the recombinant yeast with xyloseisomerase pathway
    TANINO Takanori, HOTTA Atsushi, ITO Tomonori, ISHII Jun, YAMADA Ryousuke, HASUNUMA Tomohisa, OGINO Chiaki, OHMURA Naoto
    APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference
    Poster presentation

  • Bioethanol production from mixed sugars using sugar uptake ability enhanced yeast strain by overexpression of transporters
    堀田 淳史, 谷野 孝徳, 伊藤 友数, 蓮沼 誠久, 荻野 千秋, 近藤 昭彦, 大村 直人
    APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference
    Poster presentation

  • バイオエタノール生産を目指したスーパー酵母の創製
    近藤 昭彦, 蓮沼 誠久, 田中 勉, 荻野 千秋
    農芸化学会2009年度大会, Mar. 2009, Japanese, 日本農芸化学会, 福岡県福岡市, Domestic conference
    Oral presentation

  • キシロースからの効率的なエタノール生産を行う酵母の創製
    KATO Hiroko, YAMADA Ryosuke, HASUNMA Tomohisa, FUKUDA Hideki, KONDO Akihiko
    化学工学会第74年会, Mar. 2009, Japanese, 化学工学会, 横浜市, Domestic conference
    Oral presentation

  • C3 photosynthetic pathway analysis by combination of in vivo 13C-labeling and metabolic profiling
    HASUNMA Tomohisa
    NAISTグローバルCOE公開ワークショップ「光合成の環境応答」, Mar. 2009, Japanese, 日本分子生物学会・日本生化学会, 奈良県生駒市, Domestic conference
    [Invited]
    Invited oral presentation

  • 13CO2を用いた同位体標識による葉内代謝物質のターンオーバー解析
    HASUNMA Tomohisa, HARADA Kazuo, MIYAKE Chikahiro, FUKUSAKI Ei-ichiro
    日本分子生物学会年会・日本生化学会大会 合同大会, Dec. 2008, Japanese, 日本分子生物学会・日本生化学会, 神戸市, Domestic conference
    [Invited]
    Invited oral presentation

  • in vivo安定同位体濃縮による葉内炭素代謝ターンオーバー解析
    HASUNMA Tomohisa
    メタボロームシンポジウム, Oct. 2008, Japanese, 慶應義塾大学先端生命科学研究所, 山形県鶴岡市, Domestic conference
    Poster presentation

  • in vivo 13Cエンリッチメントによる葉内炭素代謝解析システムの構築
    HASUNMA Tomohisa
    関西光合成研究会シンポジウム, Sep. 2008, Japanese, 関西光合成研究会, 神戸市, Domestic conference
    [Invited]
    Invited oral presentation

  • Development of analytical system to monitor metabolic turnoverin plant leaves using in vivo isotope dilution from 13CO2
    OHYAMA Yohko, HASUNMA Tomohisa, HARADA Kazuo, MIYAKE Chikahiro, KOBAYASHI Akio, FUKUSAKI Ei-ichiro
    5th International conference on plant metabolomics, Jul. 2008, English, RIKEN Plant Science Center, 横浜市, Domestic conference
    Poster presentation

  • 葉緑体形質転換技術を用いたアスタキサンチン生産
    HASUNMA Tomohisa, MIYAZAWA Shin-ichi, YOSHIMURA Satomi, SHINZAKI Yuki, TOMIZAWA Ken-ichi, MISAWA Norihiko, MIYAKE Chikahiro
    日本農芸化学会大会, Mar. 2008, Japanese, 日本農芸化学会, 名古屋市, Domestic conference
    Oral presentation

  • CE/MSを用いた一次代謝物の安定同位体標識率モニタリング法の開発
    HARADA Kazuo, OHYAMA Yohko, HASUNMA Tomohisa, MIYAZAWA Shin-ichi, MIYAKE Chikahiro, KOBAYASHI Akio, FUKUSAKI Ei-ichiro
    日本農芸化学会大会, Mar. 2008, Japanese, 日本生物工学会, 名古屋市, Domestic conference
    Oral presentation

  • 13CO2エンリッチメントによる代謝ターンオーバー解析
    HASUNMA Tomohisa, FUKUSAKI Ei-ichiro, OKAZAWA Atsushi, KAJIYAMA Shin-ichiro, KOBAYASHI Akio
    日本植物生理学会年会, Mar. 2008, Japanese, 日本植物生理学会, 札幌市, Domestic conference
    Oral presentation

  • 主要カロテノイドとしてアスタキサンチンを生産する葉緑体形質転換植物の作出
    HASUNMA Tomohisa, YOSHIMURA Satomi, MIYAZAWA Shin-ichi, MISAWA Norihiko, MIYAKE Chikahiro
    カロテノイド研究談話会, Sep. 2007, Japanese, 日本カロテノイド研究会, 大阪市, Domestic conference
    Oral presentation

  • 葉緑体形質転換技術を用いたカロテノイド代謝改変植物の作出
    HASUNMA Tomohisa, KAWASAKI Satomi, YAMAMOTO Hiroshi, MIYAKE Chikahiro, FUKUSAKI Ei-ichiro, TOMIZAWA Ken-ichi
    カロテノイド研究談話会, Sep. 2006, Japanese, 日本カロテノイド研究会, 沖縄県, Domestic conference
    Oral presentation

  • ラン藻Synechosystis sp.由来デオキシキシルロースリン酸レダクトイソメラーゼを導入した葉緑体形質転換タバコの作出
    HASUNMA Tomohisa, KAWASAKI Satomi, YAMAMOTO Hiroshi, TAKASE Hisabumi, TAKENO Shinya, BAMBA Takeshi, FUKUSAKI Ei-ichiro, KOBAYASHI Akio, TOMIZAWA Ken-ichi
    日本農芸化学会大会, Mar. 2005, Japanese, 日本農芸化学会, 京都市, Domestic conference
    Oral presentation

  • 安定同位体希釈法による定量メタボローム解析法の開発(2)-15N標識による含窒素化合物群の相対定量法-
    FUKUSAKI Ei-ichiro, KIM Jae Kwang, HASUNMA Tomohisa, HARADA Kazuo, KOBAYASHI Akio
    日本農芸化学会大会, Mar. 2004, Japanese, 日本農芸化学会, 広島市, Domestic conference
    Oral presentation

  • Aspergillus niger由来ペクチンメチルエステラーゼの植物内過剰発現によるメタノール増産と植物矮化
    HASUNMA Tomohisa, FUKUSAKI Ei-ichiro, OKAZAWA Atsushi, KAJIYAMA Shin-ichiro, KOBAYASHI Akio
    日本生物工学会大会, Sep. 2003, Japanese, 日本生物工学会, 熊本市, Domestic conference
    Oral presentation

  • アルコールオキシダーゼ・ペルオキシダーゼ二酵素型バイオセンサーを用いたメタノール定量測定
    HASUNMA Tomohisa, FUKUSAKI Ei-ichiro, OKAZAWA Atsushi, KAJIYAMA Shin-ichiro, KUWABATA Susumu, KOBAYASHI Akio
    日本農芸化学会大会, Mar. 2003, Japanese, 日本農芸化学会, 神奈川県藤沢市, Domestic conference
    Oral presentation

  • Aspergillus niger由来ペクチンメチルエステラーゼの植物内過剰発現によるメタノール産生
    HASUNMA Tomohisa, FUKUSAKI Ei-ichiro, OKAZAWA Atsushi, KAJIYAMA Shin-ichiro, KOBAYASHI Akio
    日本農芸化学会大会, Mar. 2002, Japanese, 日本農芸化学会, 仙台市, Domestic conference
    Oral presentation

  • チューブリン分子に特異的に結合するDNAアプタマー
    HASUNMA Tomohisa, FUKUSAKI Ei-ichiro, OKAZAWA Atsushi, KAJIYAMA Shin-ichiro, KOBAYASHI Akio
    日本生物工学会大会, Aug. 2000, Japanese, 日本生物工学会, 札幌市, Domestic conference
    Oral presentation

■ Research Themes
  • 川口 秀夫
    学術研究助成基金助成金／基盤研究(C), Apr. 2016 - Mar. 2021
    Competitive research funding

  • 蓮沼 誠久
    科学研究費補助金／若手研究(A), Apr. 2015 - Mar. 2018, Principal investigator
    Competitive research funding

  • 微細藻類からのカロテノイド色素の生産技術開発
    蓮沼 誠久
    国立研究開発法人科学技術振興機構, 研究成果展開事業 研究成果最適展開支援プログラム(A-STEP）シーズ育成タイプ, 2017, Principal investigator
    Competitive research funding

  • 【NEDO：三菱ケミカル】共同研究員受入
    近藤 昭彦
    三菱ケミカル株式会社, NEDO委託プロジェクト「植物等の生物を用いた高機能品生産技術の開発」, 2017
    Competitive research funding

  • 【JAXA地上】微小重力環境における藻類による物質循環サイクルの実現可能性検証
    蓮沼 誠久
    一般財団法人日本宇宙フォーラム, 「きぼう」利用フィジビリティスタディテーマ募集, 2017, Principal investigator
    Competitive research funding

  • 【JAXA装置】微小重力環境における藻類による物質循環サイクルの実現可能性検証
    蓮沼 誠久
    一般財団法人日本宇宙フォーラム, 「きぼう」利用フィジビリティスタディテーマ募集, 2017, Principal investigator
    Competitive research funding

  • 【ImPACT】動的代謝解析による海洋性緑藻の油脂生合成発動メカニズムの解明と油脂高生産技術開発への応用
    蓮沼 誠久
    国立研究開発法人科学技術振興機構, 革新的研究開発推進プログラム（ImPACT）, 2017, Principal investigator
    Competitive research funding

  • 【AMED】アセンブラーとしての癌/非癌幹細胞の機能解明とその制御技術の開発
    青井 貴之
    国立研究開発法人日本医療研究開発機構, 再生医療実現拠点ネットワークプログラム幹細胞・再生医学イノベーション創出プログラム, 2017
    Competitive research funding

  • 【ALCA】ラン藻代謝改変株の代謝解析とコハク酸生産プロセスの検討
    蓮沼 誠久
    国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業 先端的低炭素化技術開発（ALCA）, 2017, Principal investigator
    Competitive research funding

  • 微細藻類からのカロテノイド色素の生産技術開発
    蓮沼 誠久
    国立研究開発法人科学技術振興機構, 研究成果最適展開支援プログラム（A-STEP）, 2016, Principal investigator
    Competitive research funding

  • 宇宙空間におけるユーグレナ等の藻類による物質循環サイクルの実現可能性検証
    蓮沼 誠久
    「きぼう」利用フィジビリティスタディー, 2016, Principal investigator
    Competitive research funding

  • 【ImPACT】動的代謝解析による海洋性緑藻の油脂生合成発動メカニズムの解明と油脂高生産技術開発への応用
    蓮沼 誠久
    革新的研究開発推進プログラム（ImPACT）, 2016, Principal investigator
    Competitive research funding

  • 【ALCA】ラン藻代謝改変株の代謝解析とコハク酸生産プロセスの検討
    蓮沼 誠久
    国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業（ALCA）, 2016, Principal investigator
    Competitive research funding

  • 海洋性緑藻による油脂生産技術の研究開発
    蓮沼 誠久
    新エネルギー・産業技術総合開発機構, 戦略的次世代バイオマスエネルギー利用技術開発事業（次世代技術開発）, 2015, Principal investigator
    Competitive research funding

  • 海洋性緑藻による油脂生産技術の研究開発
    蓮沼 誠久
    独立行政法人新エネルギー・産業技術総合開発機構, 戦略的次世代バイオマスエネルギー利用技術開発事業（次世代技術開発）, 2014, Principal investigator
    Competitive research funding

  • 海洋性緑藻による油脂生産技術の研究開発
    蓮沼 誠久
    独立行政法人新エネルギー・産業技術総合開発機構, 戦略的次世代バイオマスエネルギー利用技術開発事業（次世代技術開発）, 2013, Principal investigator
    Competitive research funding

  • さきがけ「高増殖性微細藻の合成を目指した微細藻代謝フラックス制御機構の解明」
    蓮沼 誠久
    戦略的創造研究推進事業 個人型研究さきがけ, 2013, Principal investigator
    Competitive research funding

  • 海洋性緑藻による油脂生産技術の研究開発
    蓮沼 誠久
    独立行政法人新エネルギー・産業技術総合開発機構, 戦略的次世代バイオマスエネルギー利用技術開発事業（次世代技術開発）, 2012, Principal investigator
    Competitive research funding

  • さきがけ「高増殖性微細藻の合成を目指した微細藻代謝フラックス制御機構の解明」
    蓮沼 誠久
    戦略的創造研究推進事業 個人型研究さきがけ, 2012, Principal investigator
    Competitive research funding

  • さきがけ「高増殖性微細藻の合成を目指した微細藻代謝フラックス制御機構の解明」
    蓮沼 誠久
    戦略的創造研究推進事業 個人型研究さきがけ, 2011, Principal investigator
    Competitive research funding

  • 蓮沼 誠久
    科学研究費補助金／若手研究(B), 2010, Principal investigator
    Competitive research funding

■ Industrial Property Rights
  • 油脂成分を製造する方法、及び藻類を使用して高級不飽和脂肪酸を製造する方法
    近藤 昭彦, 蓮沼 誠久, 賀 詩欣, 皆川 純, 西江 晴男, 太郎田 博之, 張 嘉修
    特願14/913618, 17 Mar. 2014, 特許10351882, 16 Jul. 2019
    Patent right

  • サンプリング装置
    蓮沼 誠久, 長堀(虎井) 彩, 松本 好弘
    PCT/JP2019/027323, 10 Jul. 2019
    Patent right

  • キャップ着脱装置、並びに、これを備えたサンプリング装置及び前処理装置
    PCT/JP2019/027324, 10 Jul. 2019
    Patent right

  • 攪拌装置及び前処理装置
    蓮沼 誠久, 長堀(虎井) 彩, 松本 好弘
    PCT/JP2019/027326, 10 Jul. 2019
    Patent right

  • 攪拌装置及び前処理装置
    蓮沼 誠久, 長堀(虎井) 彩, 松本 好弘
    PCT/JP2019/027325, 10 Jul. 2019
    Patent right

  • 固液界面検出装置及びこれを備えた前処理装置
    蓮沼 誠久, 渡邊 勉, 篁 直樹
    PCT/JP2019/027327, 10 Jul. 2019
    Patent right

  • 分泌シグナルペプチドならびにそれを利用したタンパク質の分泌および細胞表層提示
    近藤 昭彦, 蓮沼 誠久, 猪熊 健太郎
    特願2016-538422, 30 Jul. 2015, 特許6537076, 14 Jun. 2019
    Patent right

  • 油脂成分を産生する方法、高級不飽和脂肪酸の製造方法、及びクラミドモナス・スピーシーズＪＳＣ４株
    近藤 昭彦, 蓮沼 誠久, 賀 詩欣, 皆川 純, 西江 晴男, 太郎田 博之, 張 嘉修
    特願14838058.7, 17 Mar. 2014, 特許3037543, 29 May 2019
    Patent right

  • クラミドモナス藻類から油脂成分を産生する方法
    近藤 昭彦, 蓮沼 誠久, 賀 詩欣, 皆川 純, 西江 晴男, 太郎田 博之, 張 嘉修
    特願14/913594, 17 Mar. 2014, 特許10214756, 26 Feb. 2019
    Patent right

  • 組換え宿主細胞及びＤ－ブタントリオールの新規製造方法
    蓮沼 誠久, 近藤 昭彦, 番場 崇弘
    PCT/JP2019/004582, 08 Feb. 2019
    Patent right

  • 油脂成分を産生する方法、高級不飽和脂肪酸の製造方法
    近藤 昭彦, 蓮沼 誠久, 賀 詩欣, 皆川 純, 西江 晴男, 太郎田 博之, 張 嘉修
    特願14837252.7, 17 Mar. 2014, 特許3037542, 13 Jun. 2018
    Patent right

  • 細胞表層発現用ポリヌクレオチド
    KONDO AKIHIKO, HASUNUMA TOMOHISA, INOKUMA KENTARO
    特願2015-508514, 25 Mar. 2014, 大学長, 特許6335161, 11 May 2018
    Patent right

  • バイオマスからのエタノールの生産方法
    KONDO AKIHIKO, HASUNUMA TOMOHISA, SAKIHAMA YURI
    特願2014-531686, 23 Aug. 2013, 大学長, 特許6236634, 10 Nov. 2017
    Patent right

  • 有機酸の製造方法
    蓮沼 誠久, 松田 真実
    PCT/JP2017/032469, 08 Sep. 2017
    Patent right

  • 有機酸の製造方法
    蓮沼 誠久, 松田 真実
    特願2018-539683, 08 Sep. 2017
    Patent right

  • 細胞表層発現用ポリヌクレオチド（米国）
    KONDO AKIHIKO, HASUNUMA TOMOHISA, INOKUMA KENTARO
    14/778250, 25 Mar. 2014, 大学長, 9751917, 05 Sep. 2017
    Patent right

  • 前処理済みの植物性バイオマスから発酵処理物を製造するための方法
    近藤 昭彦, 蓮沼 誠久, 荻野 千秋, 猪熊 健太郎, ギリモ グレゴリー, 渡部 啓吾, 安川 雄介, 金子 令治, 飯森 武志
    特願2017-77676, 10 Apr. 2017
    Patent right

  • バイオマスからのエタノールの生産方法
    KONDO AKIHIKO, HASUNUMA TOMOHISA, 崎濱 由梨
    特願14/422728, 23 Aug. 2013, 大学長, 特許9441249, 13 Sep. 2016
    Patent right

  • エタノールの新規生産方法
    KONDO AKIHIKO, HASUNUMA TOMOHISA
    特願2013-522836, 25 Jun. 2012, 大学長, 特許5935020, 20 May 2016
    Patent right

  • 有機酸の製造方法
    蓮沼 誠久
    特願2017-511006, 06 Apr. 2016
    Patent right

  • バイオマスからのエタノールの生産方法
    KONDO AKIHIKO, HASUNUMA TOMOHISA, 三田 智也
    特願2011-543349, 29 Nov. 2010, 大学長, 特許5804327, 11 Sep. 2015
    Patent right

  • 油脂成分を産生する方法、及び高級不飽和脂肪酸の製造方法
    HASUNUMA TOMOHISA, KONDO AKIHIKO, HO SHIH-HSIN
    特願2014-541229, 17 Mar. 2014, 大学長, 特許5746796, 15 May 2015
    Patent right

  • 油脂成分を産生する方法、高級不飽和脂肪酸の製造方法、及びクラミドモナス・スピーシーズＪＳＣ４株
    KONDO AKIHIKO, HASUNUMA TOMOHISA, HO SHIH-HSIN
    特願2014-550560, 17 Mar. 2014, 大学長, 特許5719977, 27 Mar. 2015
    Patent right

  • エタノールの生産方法
    KONDO AKIHIKO, HASUNUMA TOMOHISA, AIKAWA SHINPEI
    特願2010-131364, 08 Jun. 2010, 大学長, 特許5626854, 10 Oct. 2014
    Patent right

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