Research activity information

• Visualization of mechanical stress-mediated Ca²⁺ signaling in the gut using intravital imaging

  Yoshiko AIHARA, Yota FUKUDA, Akiyoshi TAKIZAWA, Naomi OSAKABE, Tomomi AIDA, Khoichi TANAKA, Soichiro YOSHIKAWA, Hajime KARASUYAMA, Takahiro ADACHI

  Mechanosensory systems have been implicated in the maintenance of gut homeostasis, but details on the related mechanisms are scarce. Recently, we generated a conditional Ca2+ biosensor yellow cameleon 3.60 (YC3.60)-expressing transgenic mouse model and established a five-dimensional (5D; x, y, z, time, and Ca2+) intravital imaging system for investigating lymphoid tissues and enteric epithelial cell responses. To validate this gut-sensing system, we visualized responses of enteric nervous system (ENS) cells in Nestin-Cre/YC3.60flox mice with specific YC3.60 expression. The ENS, including the myenteric (Auerbach's) and submucous (Meissner's) plexuses, could be visualized without staining in this mouse line, indicating that the probe produced sufficient fluorescent intensity. Furthermore, the myenteric plexus exhibited Ca2+ signaling during peristalsis without stimulation. Nerve endings on the surface of enteric epithelia also exhibited Ca2+ signaling without stimulation. Mechanical stress induced transient salient Ca2+ flux in the myenteric plexus and in enteric epithelial cells in the Nestin-Cre/YC3.60 and the CAG-Cre/YC3.60 lines, respectively. Furthermore, the potential TRPM7 inhibitors were shown to attenuate mechanical stress-mediated Ca2+ signaling. These data indicate that the present intravital imaging system can be used to visualize mechanosensory Ca2+ signaling in ENS cells and enteric epithelial cells.

  Lead, BMFH Press, 2020, Bioscience of Microbiota, Food and Health, 39(4) (4), 209 - 218, English, Domestic magazine

  [Refereed]

  Scientific journal


• Visualization of Probiotic-Mediated ca(2+) Signaling in Intestinal Epithelial Cells In Vivo

  Takahiro Adachi, Shigeru Kakuta, Yoshiko Aihara, Tomonori Kamiya, Yohei Watanabe, Naomi Osakabe, Naoki Hazato, Atsushi Miyawaki, Soichiro Yoshikawa, Takako Usami, Hajime Karasuyama, Hiromi Kimoto-Nira, Kazuhiro Hirayama, Noriko M. Tsuji

  Probiotics, such as lactic acid bacteria (LAB) and Bacillus subtilis var. natto, have been shown to modulate immune responses. It is important to understand how probiotic bacteria impact intestinal epithelial cells (IECs), because IECs are the first line of defense at the mucosal surface barrier and their activities substantially affect the gut microenvironment and immunity. However, to date, their precise mechanism remains unknown due to a lack of analytical systems available for live animal models. Recently, we generated a conditional Ca2+ biosensor Yellow Cameleon (YC3.60) transgenic mouse line and established 5D (x, y, z, time, and Ca2+) intravital imaging systems of lymphoid tissues including those in Peyer's patches and bone marrow. In the present study, we further advance our intravital imaging system for intestinal tracts to visualize IEC responses against orally administrated food compounds in real time. Using this system, heat-killed B. subtilis natto, a probiotic TTCC012 strain, is shown to directly induce Ca2+ signaling in IECs in mice housed under specific pathogen-free conditions. In contrast, this activation is not observed in the Lactococcus lactis strain C60; however, when we generate germ-free YC3.60 mice and observe the LAB stimulation of IECs in the absence of gut microbiota, C60 is capable of inducing Ca2+ signaling. This is the first study to successfully visualize the direct effect of probiotics on IECs in live animals. These data strongly suggest that probiotic strains stimulate IECs under physiological conditions and that their activity is affected by the microenvironment of the small intestine, such as commensal bacteria.

  FRONTIERS MEDIA SA, Dec. 2016, FRONTIERS IN IMMUNOLOGY, 7, 601, English

  [Refereed]

  Scientific journal


• 腸内細菌の話題 現在および未来 "管として生きる"個体の腸内環境と免疫機能

  辻 典子, 平山 和宏, 渡邉 要平, 神谷 知憲, 藍原 祥子, 角田 茂, 安達 貴弘

  (NPO)日本消化吸収学会, Oct. 2016, 消化と吸収, 39(1) (1), 32 - 32, Japanese


• Lipid peroxides as endogenous oxidants forming 8-oxo-guanosine and lipid-soluble antioxidants as suppressing agents

  Kazuki Kanazawa, Miku Sakamoto, Ko Kanazawa, Yoriko Ishigaki, Yoshiko Aihara, Takashi Hashimoto, Masashi Mizuno

  The oxidation of guanosine to 8-oxo-2'-deoxyguanosine (8-oxo-dG) in DNA is closely associated with induction of various diseases, but the endogenous oxidant species involved remains unclear. Hydrogen peroxides (H2O2) have been considered to be the oxidant, while lipid peroxides are another possible oxidant because generated easily in bio-membranes surrounding DNA. The oxidant potency was compared between lipid peroxides and H2O2. Linoleic acid hydroperoxides (LOOH) formed 8-oxo-dG at a higher level than H2O2 in guanosine or double-stranded DNA. In the presence of a physiological concentration of Fe2+ to produce hydroxyl radicals, LOOH was also a stronger oxidant. In a lipid micelle, LOOH markedly produced 8-oxo-dG at a concentration one-tenth of that of H2O2. Upon adding to rat hepatic mitochondria, phosphatidylcholine hydroperoxides produced 8-oxo-dG abundantly. Employing HepG2 cells after pretreated with glutathione peroxidase inhibitor, LOOH formed 8-oxo-dG more abundantly than H2O2. Then, antioxidants to suppress the 8-oxo-dG formation were examined, when the nuclei of pre-incubated HepG2 with antioxidants were exposed to LOOH. Water-soluble ascorbic acid, trolox, and N-acetyl cysteine showed no or weak antioxidant potency, while lipid-soluble 2,6-dipalmitoyl ascorbic acid, alpha-tocopherol, and lipid soluble phytochemicals exhibited stronger potency. The present study shows preferential formation of 8-oxo-dG upon LOOH and the inhibition by lipid-soluble antioxidants.

  JOURNAL CLINICAL BIOCHEMISTRY & NUTRITION, Jul. 2016, JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION, 59(1) (1), 16 - 24, English

  [Refereed]

  Scientific journal


• The combined effect of clothianidin and environmental stress on the behavioral and reproductive function in male mice

  Tetsushi Hirano, Shogo Yanai, Takuya Omotehara, Rie Hashimoto, Yuria Umemura, Naoto Kubota, Kiichi Minami, Daichi Nagahara, Eiko Matsuo, Yoshiko Aihara, Ryota Shinohara, Tomoyuki Furuyashiki, Youhei Mantani, Toshifumi Yokoyama, Hiroshi Kitagawa, Nobuhiko Hoshi

  Neonicotinoids, some of the most widely used pesticides in the world, act as agonists to the nicotinic acetylcholine receptors (nAChRs) of insects, resulting in death from abnormal excitability. Neonicotinoids unexpectedly became a major topic as a compelling cause of honeybee colony collapse disorder, which is damaging crop production that requires pollination worldwide. Mammal nAChRs appear to have a certain affinity for neonicotinoids with lower levels than those of insects; there is thus rising concern about unpredictable adverse effects of neonicotinoids on vertebrates. We hypothesized that the effects of neonicotinoids would be enhanced under a chronic stressed condition, which is known to alter the expression of targets of neonicotinoids, i.e., neuronal nAChRs. We performed immunohistochemical and behavioral analyses in male mice actively administered a neonicotinoid, clothianidin (CTD; 0, 10, 50 and 250 mg/kg/day), for 4 weeks under an unpredictable chronic stress procedure. Vacuolated seminiferous epithelia and a decrease in the immunoreactivity of the antioxidant enzyme glutathione peroxidase 4 were observed in the testes of the CTD+stress mice. In an open field test, although the locomotor activities were not affected, the anxiety-like behaviors of the mice were elevated by both CTD and stress. The present study demonstrates that the behavioral and reproductive effects of CTD become more serious in combination with environmental stress, which may reflect our actual situation of multiple exposure.

  JAPAN SOC VET SCI, Oct. 2015, JOURNAL OF VETERINARY MEDICAL SCIENCE, 77(10) (10), 1207 - 1215, English

  [Refereed]

  Scientific journal


• (-)-Epigallocatechin-3-gallate induces secretion of anorexigenic gut hormones

  Won-Young Song, Yoshiko Aihara, Takashi Hashimoto, Kazuki Kanazawa, Masashi Mizuno

  The anorexigenic gut hormones, cholecystokinin (CCK), glucagonlike peptide (GLP)-1 and peptide tyrosine-tyrosine (PYY), are released in response to food intake from the intestines. Dietary nutrients have been shown to stimulate these hormones. Some non-nutrients such as polyphenols show anorexigenic effects on humans. In the present study, we examined whether dietary polyphenols can stimulate secretion of these gut hormones. Caco-2 cells expressed mRNA of the gut hormones, CCK, PC1 (prohormone convertase 1), GCG (glucagon) and PYY. CCK, GLP-1 and PYY were secreted from Caco-2 cells after adding sugars, amino acids or fatty acids. Using Caco-2 cells, epigallocatechin-3-gallate (EGCG), chlorogenic acid and ferulic acid induced secretion of anorexigenic gut hormones. Particularly, EGCG induced secretion of all three hormones. In an ex vivo assay using murine intestines, EGCG also released CCK from the duodenum, and GLP-1 from the ileum. These results suggest that EGCG may affect appetite via gut hormones.

  Corresponding, JOURNAL CLINICAL BIOCHEMISTRY & NUTRITION, Sep. 2015, JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION, 57(2) (2), 164 - 169, English

  [Refereed]

  Scientific journal


• Transgenic labeling of higher order neuronal circuits linked to phospholipase C-β2-expressing taste bud cells in medaka fish

  Takashi Ieki, Shinji Okada, Yoshiko Aihara, Makoto Ohmoto, Keiko Abe, Akihito Yasuoka, Takumi Misaka

  The sense of taste plays a pivotal role in the food-selecting behaviors of vertebrates. We have shown that the fish ortholog of the phospholipase C gene (plc-β2) is expressed in a subpopulation of taste bud cells that transmit taste stimuli to the central nervous system to evoke favorable and aversive behaviors. We generated transgenic medaka expressing wheat germ agglutinin (WGA) under the control of a regulatory region of the medaka plc-β2 gene to analyze the neuronal circuit connected to these sensory cells. Immunohistochemical analysis of the transgenic fish 12 days post fertilization revealed that the WGA protein was transferred to cranial sensory ganglia and several nuclei in the hindbrain. WGA signals were also detected in the secondary gustatory nucleus in the hindbrain of 3-month-old transgenic fish. WGA signals were observed in several diencephalic and telencephalic regions in 9-month-old transgenic fish. The age-dependent increase in the labeled brain regions strongly suggests that labeling occurred at taste bud cells and progressively extended to cranial nerves and neurons in the central nervous system. These data are the first to demonstrate the tracing of higher order gustatory neuronal circuitry that is associated with a specific subpopulation of taste bud cells. These results provide insight into the basic neuronal architecture of gustatory information processing that is common among vertebrates. J. Comp. Neurol. 521:1781-1802, 2013. © 2012 Wiley Periodicals, Inc.

  Wiley Periodicals, Inc., Jun. 2013, Journal of Comparative Neurology, 521(8) (8), 1781 - 1802, English

  [Refereed]

  Scientific journal


• Construction of a taste-blind medaka fish and quantitative assay of its preference-aversion behavior

  Yoshiko Aihara, Akihito Yasuoka, Satoshi Iwamoto, Yuki Yoshida, Takumi Misaka, Keiko Abe

  In vertebrates, the taste system provides information used in the regulation of food ingestion. In mammals, each cell group within the taste buds expresses either the T1R or the T2R taste receptor for preference-aversion discrimination. However, no such information is available regarding fish. We developed a novel system for quantitatively assaying taste preference-aversion in medaka fish. In this study, we prepared fluorescently labeled foods with fine cavities designed to retain tastants until they were bitten by the fish. The subjects were fed food containing a mixture of amino acids and inosine monophosphate (AN food), denatonium benzoate (DN food) or no tastant (NT food), and the amounts of ingested food were measured by fluorescence microscopy. Statistical analysis of the fluorescence intensities yielded quantitative measurements of AN food preference and DN food aversion. We then generated a transgenic fish expressing dominant-negative G alpha(i2) both in T1R-expressing and in T2R-expressing cells. The feeding assay revealed that the transgenic fish was unable to show a preference for AN food and an aversion to DN food. The assay system was useful for evaluating taste-blind behaviors, and the results indicate that the two taste signaling pathways conveying preferable and aversive taste information are conserved in fish as well as in mammals.

  WILEY-BLACKWELL, Nov. 2008, GENES BRAIN AND BEHAVIOR, 7(8) (8), 924 - 932, English

  [Refereed]

  Scientific journal


• Transient receptor potential channel M5 and phospholipaseC-beta 2 colocalizing in zebrafish taste receptor cells

  Yuki Yoshida, Kana Saitoh, Yoshiko Aihara, Shinji Okada, Takumi Misaka, Keiko Abe

  In mammals, transient receptor potential (TRP) channel M5 (TRPM5) is coexpressed with phospholipaseC-beta 2 (PLC-beta 2) in the taste receptor cells, and both PLC-beta 2 and TRPM5 are essential elements in the signal transduction of sweet, bitter and umami stimuli. In this study, we identified the zebrafish homologue of TRPMS (zfFRPM5) and examined its expression in the gustatory system by in-situ hybridization. Using a transgeric zebrafish line that expressed green fluorescent protein under the control of the PLC-beta 2 promoter, we showed that zfTRPM5 is expressed in green fluorescent protein-labeled cells of the taste buds. These results demonstrate that zfTRPM5 and PLC-beta 2 colocalize in zebrafish taste receptor cells, suggesting their crucial roles in taste signaling via the fish taste receptors.

  LIPPINCOTT WILLIAMS & WILKINS, Oct. 2007, NEUROREPORT, 18(15) (15), 1517 - 1520, English

  [Refereed]

  Scientific journal


• Characterization of ligands for fish taste receptors

  Hideaki Oike, Toshitada Nagai, Akira Furuyama, Shinji Okada, Yoshiko Aihara, Yoshiro Ishimaru, Takayuki Marui, Ichiro Matsumoto, Takumi Misaka, Keiko Abe

  Recent progress in the molecular biology of taste reception has revealed that in mammals, the heteromeric receptors T1R1/3 and T1R2/3 respond to amino acids and sweeteners, respectively, whereas T2Rs are receptors for bitter tastants. Similar taste receptors have also been characterized in fish, but their ligands have not been identified yet. In the present study, we conducted a series of experiments to identify the fish taste receptor ligands. Facial nerve recordings in zebrafish ( Danio rerio) demonstrated that the fish perceived amino acids and even denatonium, which is a representative of aversive bitter compounds for mammals and Drosophila. Calcium imaging analysis of T1Rs in zebrafish and medaka fish ( Oryzias latipes) using an HEK293T heterologous expression system revealed that both T1R1/3 and a series of T1R2/3 responded to amino acids but not to sugars. A triple-labeling, in situ hybridization analysis demonstrated that cells expressing T1R1/3 and T1R2/3s exist in PLC beta 2-expressing taste bud cells of medaka fish. Functional analysis using T2Rs showed that zfT2R5 and mfT2R1 responded to denatonium. Behavior observations confirmed that zebrafish prefer amino acids and avoid denatonium. These results suggest that, although there may be some fish-specific way of discriminating ligands, vertebrates could have a conserved gustatory mechanism by which T1Rs and T2Rs respond to attractive and aversive tastants, respectively.

  SOC NEUROSCIENCE, May 2007, JOURNAL OF NEUROSCIENCE, 27(21) (21), 5584 - 5592, English

  [Refereed]

  Scientific journal


• Transgenic labeling of taste receptor cells in model fish under the control of the 5 '-upstream region of medaka phospholipase C-beta 2 gene

  Yoshiko Aihara, Akihito Yasuoka, Yuki Yoshida, Makoto Ohmoto, Akiko Shimizu-Ibuka, Takumi Misaka, Makoto Furutani-Seiki, Ichiro Matsumoto, Keiko Abe

  Vertebrate taste receptor cells express signaling molecules such as taste receptors and effectors to convert taste stimuli to inner cellular signals. Phospholipase C-beta 2 (PLC-beta 2) is an effector enzyme that is necessary to transduce taste signals in the mouse. It was shown that a subset of the plc-beta 2 expressing cells also express taste receptor molecules, T1Rs or T2Rs, in mammals and fish. To label plc-beta 2 expressing cells in the model fish species, we constructed a transgene by linking the 5'-upstream region of the medaka plc-beta 2 gene to a green fluorescent protein (GFP) gene. The resulting transgenic medaka exhibited GFP signals in taste buds of the lips and the pharyngeal region. Detailed observation revealed that the GFP signals were in a subpopulation of taste bud cells, and co-localized with the transcript of endogenous plc-beta 2 gene. Zebrafish introduced with the same transgene showed GFP signals in a subpopulation of taste bud cells of the lips and the pharyngeal region as in the case of medaka. This is the first report of successful labeling of taste receptor cells in two model fish species under the control of the plc-beta 2 promoter. This promoter will be a useful genetic tool to study the vertebrate taste system in general. (c) 2006 Elsevier B.V. All rights reserved.

  ELSEVIER SCIENCE BV, Jan. 2007, GENE EXPRESSION PATTERNS, 7(1-2) (1-2), 149 - 157, English

  [Refereed]

  Scientific journal


• Phospholipase C-beta 2 as a mammalian taste signaling marker is expressed in the multiple gustatory tissues of medaka fish, Oryzias latipes

  Akihito Yasuoka, Yoshiko Aihara, Ichiro Matsumoto, Keiko Abe

  Phospholipase C-beta 2 (PLC-beta 2) is a key enzyme in mammalian taste signal transduction. To analyze the taste system in fishes at molecular level, we cloned mfplc-beta 2 as a medaka fish homologue of PLC-beta 2. In situ hybridization analysis revealed that mfplc-beta 2 is expressed in the lip and branchial region where chemosensory tissues are distributed. Immunohistochemical detection of nerve fibers near the mfplc-beta 2 positive cells suggests the characteristic of peripheral sensory cells. These results suggest that mfplc-beta 2 is expressed in the gustatory sensory cells of medaka. This may provide a molecular basis for the taste reception at multiple tissues in fish species. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

  Lead, ELSEVIER SCIENCE BV, Jul. 2004, MECHANISMS OF DEVELOPMENT, 121(7-8) (7-8), 985 - 989, English

  [Refereed]

  Scientific journal


• Mutations affecting the formation of posterior lateral line system in Medaka, Oryzias latipes

  Akihito Yasuoka, Yukihiro Hirose, Hiroki Yoda, Yoshiko Aihara, Hiroshi Suwa, Katsutoshi Niwa, Takao Sasado, Chikako Morinaga, Tomonori Deguchi, Thorsten Henrich, Norimasa Iwanami, Sanae Kunimatsu, Keiko Abe, Hisato Kondoh, Makoto Furutani-Seiki

  We performed a systematic screen for mutations affecting the trajectory of axons visualized by immunohistochemical staining of Medaka embryos with anti-acetylated tubulin antibody. Among the mutations identified, yanagi (yan) and kazura (kaz) mutations caused specific defects in projection of the posterior lateral line (PLL) nerve. In yan and kaz mutant embryos, the PLL nerve main bundle was misrouted ventrally and dorsally or anteriorly. Medaka semaphorin3A, sdf1, and cxcr4 cDNA fragments were cloned to allow analysis of these mutants. There were no changes in semaphorin3A or sdf1 expression in mutant embryos, suggesting that the tissues expressing semaphorin3A or sqf1 that are involved in PLL nerve guidance are present in these mutant embryos. Double staining revealed that the mislocated PLL primordium and growth cone of the ectopically projected PLL nerve were always colocalized in both yan and kaz mutant embryos, suggesting that migration of PLL primordia and PLL nerve growth cones are not uncoupled in these mutants. Although homozygous yan larvae showed incomplete migration of the PLL primordium along the anteroposterior axis, ventral proneuromast migration was complete, suggesting that ventral migration of the proneuromast does not require the signaling affected in yan mutants. In addition to the PLL system, the distribution of primordial germ cells (PGCs) was also affected in both yan and kaz mutant embryos, indicating that yan and kaz genes are required for the migration of both PLL primordia and PGCs. Genetic linkage analysis indicated that kaz is linked to cxcr4, but yan is not linked to sdf1 or cxcr4. These mutations will provide genetic clues to investigate the molecular mechanism underlying formation of the PLL system. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

  ELSEVIER SCIENCE BV, Jul. 2004, MECHANISMS OF DEVELOPMENT, 121(7-8) (7-8), 729 - 738, English

  [Refereed]

  Scientific journal

• Bridge between taste and functionality of food

  AIHARA YOSHIKO

  Jun. 2015, Journal of Japanese Society for Medical Use of Functional Foods, 9(2) (2), 61 - 66, Japanese

  [Invited]

  Introduction scientific journal


• ウミガメの総排泄腔傍嚢の形態機能学的特徴

  辰巳敬哉, 亀崎直樹, 藍原祥子, 山下真路, 谷口真理, 三根佳奈子, 坂本未来, 表原拓也, 小林泰丈, 横山俊史, 北川浩, 今川智敬, 星信彦

  30 Aug. 2013, 日本獣医学会学術集会講演要旨集, 156th, 191, Japanese


• メダカから探る味覚系の基本原理

  安岡顕人, 家木誉史, 岡田晋治, 藍原祥子, 應本真, 三坂巧, 阿部啓子

  Jun. 2013, 小児耳鼻咽喉科, 34(2) (2), 85, Japanese


• カメ類における「膀胱」の形態機能学的研究

  辰巳敬哉, 亀崎直樹, 藍原祥子, 山下真路, 谷口真理, 三根佳奈子, 坂本未来, 木下今日子, 横山俊史, 北川浩, 今川智敬, 金沢和樹, 星信彦

  31 Mar. 2013, は虫両棲類学会報, 2013(1) (1), 66, Japanese


• カメ類における「膀胱」の形態機能学的定義

  辰巳敬哉, 亀崎直樹, 藍原祥子, 山下真路, 谷口真理, 三根佳奈子, 坂本未来, 表原拓也, 小林泰丈, 横山俊史, 北川浩, 今川智敬, 星信彦

  2013, 日本野生動物医学会大会・講演要旨集, 19th, 98, Japanese


• Transient Receptor Potential Channel M5 and Phospholipasec-beta 2 CO-Localizing in Zebrafish Taste Receptor Cells

  Yuki Yoshida, Kana Saitoh, Yoshiko Aihara, Shinji Okada, Takumi Misaka, Keiko Abe

  OXFORD UNIV PRESS, Oct. 2008, CHEMICAL SENSES, 33(8) (8), S146 - S146, English

  Summary international conference

• 茶ポリフェノールの生理機能と応用展開

  立花 宏文 仲川 清隆 小林 誠 藤村 由紀 山下 修矢 竹下 尚男 熊澤 茂則 海野 けい子 藍原 祥子 熊添 基文 白上 洋平 清水 雅仁 鈴木 拓史 三好 規之 宮本 敬久 吉富 廉 赤川 貢 内田 浩二 川畑 球一 山本(前田) 万里 和才 昌史 田村 宗明 本田 義知 田中 知成 田中 浩士 平井 瑞輝 小堀 亮 道下 僚 齊藤 安貴子 福原 潔 水野 美麗 原 征彦 田中 隆 松尾 洋介 中山 勉 奈良井 朝子 中山 幹男 高見澤 菜穂子 矢澤 一良 竹元 万壽美 武政 徹 西川 恵三

  Joint work, エピガロカテキンガレートによる消化管由来の摂食抑制ホルモン分泌の亢進, シーエムシー出版, Sep. 2019, Japanese, ISBN: 9784781314334

• 抗生物質誘導性腸内細菌叢枯渇マウスの味覚嗜好性変化

  藍原祥子, 丸岡祐子

  日本味と匂学会第54回大会, Oct. 2020


• 腸内細菌枯渇状態のマウスの味覚嗜好性変化

  丸岡 祐子, 藍原 祥子

  日本農芸化学会2019年度大会, 2020年, Mar. 2020


• メダカを用いた食品・医薬品の機能性評価基盤の構築

  藍原 祥子

  日本栄養・食糧学会第73回大会, May 2019, 味覚受容システムの解明/シンポジウム「メダカを用いた食品・医薬品の機能性評価基盤の構築」


• 鉄欠乏マウスにおける酸味細胞の変化

  奥野葉月, 藍原祥子

  日本農芸化学会2018年度大会, Mar. 2019


• Alteration of taste sensitivity in iron deficient mice

  OKUNO Hazuki, AIHARA YOSHIKO

  日本味と匂学会第52回大会, Oct. 2018, English, 大宮ソニックシティ, Domestic conference

  Poster presentation


• カテキンに惹起される内分泌様培養細胞における細胞内カルシウムシグナル伝達経路の解析

  岸田 佳織, AIHARA YOSHIKO, HASHIMOTO TAKASHI, MIZUNO MASASHI

  日本農芸化学会関西支部第498回講演会, Feb. 2017, Japanese, 京都大学百周年時計台記念館, Domestic conference

  Oral presentation


• Analysis of gut hormone secretion induced by food compounds

  AIHARA YOSHIKO

  第6回 オルソオルガノジェネシス検討会, Dec. 2016, English, 恩納村、沖縄, International conference

  Oral presentation


• マラリア原虫Plasmodium berghei感染ICRマウスにおける硫酸化多糖の抗マラリア効果

  YUTA KASHO, TAKASHI HASHIMOTO, YOSHIKO AIHARA, MIZUNO MASASHI

  日本農芸化学会平成28年度大会, Mar. 2016, Japanese, 札幌, Domestic conference

  Poster presentation


• アリルイソチオシアネート代謝物が肝細胞の脂質蓄積に及ぼす影響に関する研究

  SAYO AMINO, TAKASHI HASHIMOTO, SHINO NAKAMURA, YOSHIKO AIHARA, MIZUNO MASASHI

  日本農芸化学会平成28年度大会, Mar. 2016, Japanese, 札幌, Domestic conference

  Poster presentation


• The anti-allergic effects of enterococcus faecalis through toll-like receptor 2

  SAE YAMASHITA, YUKI YOKOYAMA, TAKASHI HASHIMOTO, YOSHIKO AIHARA, MIZUNO MASASHI

  Proceedings of the 19th international conference, Nov. 2015, English, Kobe, International conference

  Poster presentation


• Oral intake of F-fucoidan from Laminaria Japonica prevents type I allergy through secretion of galectin-9

  YUKA TANINO, TAKASHI HASHIMOTO, YOSHIKO AIHARA, TAKAO OJIMA, MIZUNO MASASHI

  Proceedings of the 19th international conference, Nov. 2015, Japanese, Kobe University, International conference

  Poster presentation


• マコンブ由来F-フコイダンによる新規Ⅰ型アレルギー抑制機構

  YUKA TANINO, TAKASHI HASHIMOTO, YOSHIKO AIHARA, TAKAO OJIMA, MIZUNO MASASHI

  日本応用糖質科学会平成２７年度大会, Sep. 2015, Japanese, 奈良, 【目的】これまでに我々はマコンブに含まれるF-フコイダンがⅠ型アレルギー抑制効果を有しており、その効果にガレクチン9が関与していることを明らかにしてきた。ガレクチン9はβガラクトシドを認識するレクチンの一種で、近年IgEと結合することでIgEの肥満細胞への結合を阻害することが報告されている。今回、我々は実際にF-フコイダンによりガレクチン9が血中で増加してⅠ型アレルギー症状を抑制しているのかを検討した。 【方法】F-フコイダン(200 μg/日)を4日間胃内強制投与した。抗原特異的IgEを尾静脈から投与し、30分後に耳介厚を測定、抗原を耳介に塗布した。その2時間後に再び耳介厚を測定し、抗原刺激前後の耳介厚の差を浮腫として表した。F-フコイダンにより誘導されたガレクチン9の関与を調べるため、ガレクチン9のアンタゴニストであるラクトースを抗原特異的IgE, Domestic conference

  Oral presentation


• 食品由来成分による消化管ホルモン分泌に関する細胞内メカニズム

  AIHARA YOSHIKO, SONG WON YOUNG, KISHIDA KAORI, MIZUNO MASASHI

  第15回AOB研究会, Jul. 2015, Japanese, 大阪, Domestic conference

  Oral presentation


• 鉄欠乏マウスにおける味覚嗜好性の変化

  MIYAMOTO TAKESHI, AIHARA YOSHIKO, HASHIMOTO TAKASHI, MIZUNO MASASHI

  日本農芸化学会平成27年度大会, Mar. 2015, Japanese, 岡山大学, Domestic conference

  Oral presentation


• ルテオリンの炎症抑制効果に対するフラボノイド同時摂取の影響

  YAMASHITA SAE, AIHARA YOSHIKO, HASHIMOTO TAKASHI, MIZUNO MASASHI

  日本農芸化学会平成27年度大会, Mar. 2015, Japanese, 岡山大学, Domestic conference

  Oral presentation


• 腸管セロトニン産生細胞におけるTRPチャンネルの発現解析

  TAKIZAWA AKIYOSHI, AIHARA YOSHIKO, HASHIMOTO TAKASHI, MIZUNO MASASHI

  日本農芸化学会関西支部第488回講演会, Jan. 2015, Japanese, 京都大学楽友会館, Domestic conference

  Oral presentation


• エピガロカテキンガレートはGLP-1を介して血糖値を調整する

  ODA AKANE, AIHARA YOSHIKO, HASHIMOTO TAKASHI, MIZUNO MASASHI

  日本農芸化学会関西支部第488回講演会, Jan. 2015, Japanese, 京都大学楽友会館, Domestic conference

  Oral presentation


• 6-メチルスルフィニルヘキシルイソチオシアネートの細胞周期開始抑制機序に関する研究

  小寺 裕貴, HASHIMOTO TAKASHI, 石村 麻耶, AIHARA YOSHIKO, 金沢 和樹, MIYAKE HIDEYOSHI, YOSHIDA MASARU, MIZUNO MASASHI

  日本農芸化学会 関西支部 支部例会（第487回講演会）, Dec. 2014, Japanese, 神戸大学農学部C101教室（神戸）, Domestic conference

  Oral presentation


• SHRSP5/Dmcrラットにおける非アルコール性脂肪性肝疾患に対するクロロゲン酸の抑制効果

  宮崎 泰一, HASHIMOTO TAKASHI, AIHARA YOSHIKO, 北森 一哉, MIZUNO MASASHI

  第19回 日本フードファクター学会学術集会, Nov. 2014, Japanese, 鹿児島大学郡元キャンパス（鹿児島）, Domestic conference

  Oral presentation


• 抗炎症効果を有するルテオリンの吸収促進物質の探索

  山下 紗枝, HASHIMOTO TAKASHI, AIHARA YOSHIKO, MIZUNO MASASHI

  日本食品科学工学会 第61回大会, Aug. 2014, Japanese, 中村学園大学（福岡）, Domestic conference

  Oral presentation


• マコンブ由来F-フコイダンの抗アレルギー効果

  谷野 有佳, AIHARA YOSHIKO, HASHIMOTO TAKASHI, 尾島 孝男, MIZUNO MASASHI

  日本食品科学工学会 第61回大会, Aug. 2014, Japanese, 中村学園大学（福岡）, Domestic conference

  Oral presentation


• 消化管ホルモンGLP-1を介したAOBの生理効果

  小田 あかね, 宋 苑昤, AIHARA YOSHIKO, 金沢 和樹, HASHIMOTO TAKASHI, MIZUNO MASASHI

  第14回 AOB研究会, Jun. 2014, Japanese, ホテル日航福岡（福岡）, Domestic conference

  Oral presentation


• ルチン経口投与によるマウス薬物代謝第Ⅱ相酵素に及ぼす影響

  岩見 志歩, HASHIMOTO TAKASHI, AIHARA YOSHIKO, MIZUNO MASASHI

  第68回 日本栄養・食糧学会大会, May 2014, Japanese, 明治大学生田キャンパス（神奈川県川崎市）, Domestic conference

  Oral presentation


• タマネギ外皮抽出物のStaphylococcus aureusに対する抗菌効果に関する研究

  黄 達, HASHIMOTO TAKASHI, AIHARA YOSHIKO, 金沢 和樹, MIZUNO MASASHI

  日本農芸化学会2014年度大会, Mar. 2014, Japanese, 明治大学生田キャンパス（神奈川県川崎市）, Domestic conference

  Oral presentation


• フコキサンチノールが誘導するアポトーシスはアクチン重合を伴う

  大原 佳樹, HASHIMOTO TAKASHI, AIHARA YOSHIKO, 金沢 和樹, MIZUNO MASASHI

  日本農芸化学会 関西支部 支部例会（第482回講演会）, Dec. 2013, Japanese, 神戸大学農学部C101教室（神戸）, Domestic conference

  Oral presentation


• ウミガメの総排泄腔傍嚢の形態機能学的特徴

  辰巳 敬哉, 亀崎 直樹, AIHARA YOSHIKO, 山下 真路, 谷口 真理, 三根 佳奈子, 坂本 未来, 表原 拓也, 小林 泰丈, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, 今川 智敬, HOSHI NOBUHIKO

  第156回日本獣医学会学術集会, Sep. 2013, Japanese, 岐阜市, Domestic conference

  Oral presentation


• Dietary polyphenols induce a secretion of gut hormones from Caco-2 cells

  SONG WON YOUNG, AIHARA YOSHIKO, HASHIMOTO TAKASHI, KANAZAWA KAZUKI

  20th International Congress of Nutrition, Sep. 2013, English, Granada, International conference

  Poster presentation


• A Co-administration of flavonoids to increase their bioavailability in rat

  Ayumi Doi, Yoshiko Aihara, Takuro Momota, Akiyuki Yokoyama, Takashi Hashimoto, Kazuki Kanazawa

  IUNS 20th International Congress of Nutrition, Sep. 2013, English, Granada Congress Centre, Granada, Spain, International conference

  Poster presentation


• 6-Methysulfinyl hexylisothiocyanate inhibits cell cycle progression accompanied by interaction with glyceraldehyde-3-phosphate dehydrogenase

  Maya Ishimura, HASHIMOTO TAKASHI, Maki Tanaka, Yoshiko Aihara, Kazuki Kanazawa

  IUNS 20th International Congress of Nutrition, Sep. 2013, English, Granada Congress Centre, Granada, Spain, International conference

  Poster presentation


• カメ類における「膀胱」の形態機能学的定義

  辰巳 敬哉, 亀崎 直樹, AIHARA YOSHIKO, 山下 真路, 谷口 真理, 三根 佳奈子, 坂本 未来, 表原 拓也, 小林 泰丈, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, 今川 智敬, HOSHI NOBUHIKO

  第19回日本野生動物医学会大会, Aug. 2013, Japanese, 京都市, Domestic conference

  Poster presentation


• 腸上皮様培養細胞の消化管ホルモン分泌に対するAOBの効果

  SONG Wong Yong, AIHARA Yoshiko, HASHIMOTO Takashi, KANAZAWA Kazuki, MIZUNO Masashi

  第13回 AOB研究会, Jul. 2013, Japanese, 東京, Domestic conference

  Oral presentation


• 食事ポリフェノールによる消化管ホルモンの分泌調節

  SONG Wong Yong, AIHARA Yoshiko, HASHIMOTO Takashi, KANAZAWA Kazuki

  第67回日本栄養・食糧学会大会, May 2013, Japanese, 名古屋, Domestic conference

  Oral presentation


• カメ類における「膀胱」の形態機能学的研究

  辰巳 敬哉, 亀崎 直樹, AIHARA YOSHIKO, 山下 真路, 谷口 真理, 三根 佳奈子, 坂本 未来, 木下 今日子, YOKOYAMA TOSHIFUMI, KITAGAWA HIROSHI, 今川 智敬, KANAZAWA KAZUKI, HOSHI NOBUHIKO

  第51回日本爬虫類両棲類学会, Nov. 2012, Japanese, 豊田市, Domestic conference

  Oral presentation


• 腸管上皮モデルを用いた香辛料成分による消化管ホルモンの分泌の検討

  SONG Wong Yong, AIHARA Yoshiko, HASHIMOTO Takashi, KANAZAWA Kazuki

  第27回日本香辛料研究会, Oct. 2012, Japanese, 藤沢, Domestic conference

  Oral presentation


• マウス黒色腫細胞におけるメラニン産生に対するAOBの抑制効果

  AIHARA Yoshiko, MATSUSHIRO Koharu, HASHIMOTO Takashi, MIZUNO Masashi, KANAZAWA Kazuki

  第12回AOB研究会, Jun. 2012, Japanese, 京都, Domestic conference

  Oral presentation


• ポリフェノール刺激によるヒト腸管由来培養細胞における消化管ホルモンの分泌

  AIHARA Yoshiko, Song Won Young, HASHIMOTO Takashi, KANAZAWA Kazuki

  第66回日本栄養・食糧学会大会, May 2012, Japanese, 仙台, Domestic conference

  Oral presentation


• Constructing a taste behavior assay method using small fish

  AIHARA Yoshiko, YASUOKA Akihito, IWAMOTO Satoshi, YOAHIDA Yuki, WATANABE Michiko, MISAKA Takumi, ABE Keiko

  The 9th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception (YR Umami Forum2011), Nov. 2011, English, Fukuoka, International conference

  Oral presentation

• THE JAPANESE ASSOCIATION FOR THE STUDY OF TASTE AND SMELL


• JAPAN SOCIETY OF NUTRITION AND FOOD SCIENCE


• JAPAN SOCIETY FOR BIOSICENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY

• Visualization for reception of poorly absorbable food compounds on intestinal epithelia

  藍原 祥子

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Kobe University, Apr. 2020 - Mar. 2024

  本研究は、難消化性ながら身体に影響を及ぼす食品成分の機能として腸管上皮による受容と腸脳相関に着目し、作用機序を明らかにするものである。 ①本年度は昨年度に引き続き、腸上皮での受容を経時的に解析するシステムとして、マウスを用いた腸管上皮のライブイメージング系の実験を行った。前年までに特異的なカルシウムセンサーの導入により内分泌細胞の応答を可視化することに成功したが、組織の動きが大きく画像処理が非常に困難であるという問題が生じた。本年度はその解決を目指し、麻酔薬の利用やデバイスの作成を検討した。現在のところ、再現度の高い実験系の構築には至っておらず、今後は新たに研究協力を要請することで解決を試みる。 ②難消化性食品成分の受容体候補を探索するにあたり、分子ドッキングシミュレーションを用いて結合を予測することを検討した。腸管ホルモンを分泌することが知られている複数の食品成分に対し、腸管で発現する感覚受容体との結合を、新たに予測された構造をもとに計算し結合エネルギーを求めた。その結果、生化学的な結合の強さを正確に予測しうる計算が困難であると結論された。今後は生化学実験を中心に行いながら、引き続き実用的なシミュレーションの方法を探索する。


• 麦生理活性成分に対する腸管上皮における応答の解析

  エリザベス・アーノルド富士財団研究助成, Aug. 2020 - Mar. 2022, Principal investigator

  Competitive research funding


• 匂いによる母子間交流を活用した安全・安心の創造

  尾崎 まみこ

  科学技術振興機構, 戦略的な研究開発の推進 未来社会創造事業 探索加速型, 奈良女子大学, 2021 - 2022, Coinvestigator

  私たちは、出産間もない母子が相互交流に用いている「匂い」の効用に着目し、これを再現した香料を様々に使って、周産期母子の心身の問題解決を念頭に、そのような「匂い」の未知の価値を見出す。私たちは既に、新生児に胎内での安全・安心の記憶を喚起する「母親の羊水の匂い」、 また、母親にポジティブなケア情動を引き起こす「新生児の頭の匂い」の化学組成の同定を行い、世界で初めて調香品を作成した。本研究開発事業では、「母親の羊水の匂い」と「新生児の頭の匂い」の作用を多面的に追究する一方、調香品を用いてその作用を新生児や母親にフィードパックすることで、周産期における母子の不安やストレスの解消を目指す。さらに、その作用が母子に限ることなく、より広範に働くのであれば、あらゆる世代、あらゆる働き手にもポジティブな癒しの効果をもたらし、生きがい働きがいを高めることが期待できる。


• 麦ポリフェノールの腸管での受容を介した三次機能の評価

  藍原 祥子

  エリザベス・アーノルド富士財団, エリザベス・アーノルド富士財団研究助成, Apr. 2019 - Mar. 2020, Principal investigator

  Competitive research funding