Research activity information

• 2022 The Society for Biotechnology, Japan, Society Award, Innovative approaches to creating smart cells for bioproduction

  Akihiko Kondo


• Oct. 2020 神戸大学, 令和2年度 学長表彰（財務貢献者）

  近藤 昭彦


• 2017 International Bioprocessing Association, Outstanding Scientist Award-2016

  KONDO Akihiko


• 2016 American Institute for Medical and Biological Engineering (AIMBE), Fellow

  KONDO Akihiko


• 2016 膜シンポジウム2016, 膜シンポジウム 学生賞, バイオエタノール生産プロセスにおける浸透圧駆動型膜分離法を利用した糖液濃縮の検討

  渋谷 真史, YASUKAWA MASAHIRO, 佐々木 建吾, 田中 裕大, TAKAHASHI TOMOKI, KONDO AKIHIKO, MATSUYAMA HIDETO

  Japan society


• 2016 IMSTEC2016, IMSTEC2016 Travel award, Up-concentration of sugar solution by using forward osmosis for bioethanol production process

  渋谷 真史, YASUKAWA MASAHIRO, 佐々木 建吾, 田中 裕大, TAKAHASHI TOMOKI, KONDO AKIHIKO, MATSUYAMA HIDETO

  International society


• 2015 The Association of American Publishers, PROSE Awards, Winner in Environmental Science, Bioprocessing of Renewable Resources to Commodity Bioproducts

  KONDO Akihiko


• Jan. 2014 The Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA), Award for Excellence to Authors Publishing in BBB, Utilization of Lactic Acid Bacterial Genes in Synechocystis sp. PCC 6803 in the Production of Lactic Acid

  Ancy Joseph, Simpei Aikawa, Kengo Sasaki, Yota Tsuge, Fumio Matsuda, Tsutomu Tanaka, Akihiko Kondo

  Japan society


• Sep. 2013 経済産業省, 「ものづくり日本大賞」経済産業大臣賞（製品・技術開発部門）, 世界初マイクロチップでの単一細胞全自動解析・回収装置の商品化開発

  徐 杰, 木村 健一, 金野 徹, 松下 政宏, 黒田 俊一, 近藤 昭彦, 藤井 郁雄


• Sep. 2013 The Society for Biotechnology, Japan, Excellent Paper Award, Direct isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display system

  SOMA YUKI, INOKUMA KENTARO, TANAKA TSUTOMU, OGINO CHIAKI, KONDO AKIHIKO, OKAMOTO MASAHIRO, HANAI TAIZO

  Japan society


• 2011 Iue Memorial Foundation, Iue Memorial Foundation Award

  KONDO Akihiko


• Oct. 2010 The Society for Biotechnology, Japan, Achievement Award, 細胞表層工学技術の広範な展開と合成生物工学の開拓によるバイオ燃料・グリーン化学品生産のための細胞工場の創製―バイオリファイナリーの構築を目指して―

  KONDO Akihiko


• Jul. 2010 Renewable Energy 2010, Best Paper Award, Construction of consolidated biopreocesses for production of bio-fuels and chemicals by using cell surface engineered microbial cells

  KONDO Akihiko


• Oct. 2009 神戸大学, 第1回学長表彰

  近藤 昭彦


• Nov. 2008 日刊工業新聞社, モノづくり連携大賞 特別賞

  近藤 昭彦


• Sep. 2008 竹田理化工業株式会社, 竹田国際貢献賞

  近藤 昭彦


• 2007 フジサンケイビジネスアイ, 第21回 独創性を拓く 先端技術大賞 特別賞, 熱応答性磁性ナノ粒子（Therma-Max）の開発とその実用化～産学連携がもたらした若手研究者たちの挑戦～

  大西 徳幸, 畑 英之, 松井 景明, 近藤 昭彦


• 2005 日本イージェイケー株式会社, オルガテクノ大賞 材料・素材部門賞, 熱応答性磁性ナノ粒子 商標名：Therma-Max

  大西 徳幸, 近藤 昭彦


• 2004 Biotechnology Japan, Bio Business Competition Japan, Best award, 熱応答性磁性ナノ粒子(商標名：Therma-Max)の開発とその実用化

  大西 徳幸, 近藤 昭彦


• 2001 The Society for Biotechnology, Japan, Excellent Paper Award, Improvement of productivity of active horseradish peroxidase in Escherichia coli by coexpression of Dsb proteins

  Akihiko Kondo, Jiro Kohda, Yasunori Endo, Tokuhisa Shiromizu, Yoichi Kurokawa, Kazuyo Nishihara, Hideki Yanagi, Takashi Yura, Hideki Fukuda


• 2000 The Society for Biotechnology, Japan, Excellent Paper Award, Biodiesel fuel production from plant oil catalyzed by Rhizopus oryzae lipase in a water-containing system without an organic solvent

  Masaru Kaieda, Taichi Samukawa, Takeshi Matsumoto, Kazuhiro Ban, Akihiko Kondo, Yuji Shimada, Hideo Noda, Fumiki Nomoto, Koutaro Ohtsuka, Eiji Izumoto, Hideki Fukuda


• 1999 The Society for Biotechnology, Japan, Excellent Paper Award, Protein refolding system using holo-chaperonin from the thermophilic bacterium Thermus thermophilus

  Tadanaru Teshima, Jiro Kohda, Akihiko Kondo, Hideki Taguchi, Masafumi Yohda, Isao Endo, Hideki Fukuda


• 1993 The Society of Chemical Engineers, Excellent young scientist award, 微粒子材料の生物化学工学への応用に関する研究

  KONDO Akihiko

• Development of a Quantitative Serial LC-MS/MS Method for Gut Microbiota Metabolomics

  Takanobu Yoshida, Tomoya Shintani, Daisuke Sasaki, Christopher J. Vavricka, Yasushi Matsuki, Akihiko Kondo, Tomohisa Hasunuma

  Mar. 2026, ACS Omega

  Scientific journal


• Astaxanthin Overproduction Enhanced by Metabolomics-Guided Rational Metabolic Engineering in Synechococcus sp. PCC 7002

  Kousuke Ida, Kenya Tanaka, Yuichi Kato, Nobuaki Koike, Yoji Horie, Mami Matsuda, Hisashi Yasueda, Akihiko Kondo, Tomohisa Hasunuma

  Nov. 2025, ACS Synthetic Biology

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Identification of Subfamily Specific Residues within Highly Active and Promiscuous Alcohol Dehydrogenases

  Ryota Hidese, Kanae Sakai, Musashi Takenaka, Keiji Fushimi, Hisashi Kudo, Kenya Tanaka, Ryo Nasuno, Christopher J. Vavricka, Akihiko Kondo, Tomohisa Hasunuma

  Jul. 2025, ACS Catalysis

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Metabolic Engineering for Resveratrol Production Based on Modularization of Metabolic Pathways in Escherichia coli

  Daisuke Nonaka, Mayumi Kishida, Yuuki Hirata, Ayana Mori, Akihiko Kondo, Yutaro Mori, Shuhei Noda, Tsutomu Tanaka

  May 2025, Journal of Agricultural and Food Chemistry

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Combinatorial Nonribosomal Peptide Synthetase Libraries Using the SEAM-Combi-OGAB Method

  Varada Jagadeesh, Nobuyuki Okahashi, Fumio Matsuda, Kenji Tsuge, Akihiko Kondo

  Feb. 2025, ACS Synthetic Biology

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• De Novo Production of the Bioactive Phenylpropanoid Artepillin C Using Membrane-Bound Prenyltransferase in Komagataella phaffii

  Takahiro Bamba, Ryosuke Munakata, Yuya Ushiro, Ryota Kumokita, Sayaka Tanaka, Yoshimi Hori, Akihiko Kondo, Kazufumi Yazaki, Tomohisa Hasunuma

  Dec. 2024, ACS Synthetic Biology

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Parallel metabolic pathway engineering for aerobic 1,2‐propanediol production in Escherichia coli

  Daisuke Nonaka, Yuuki Hirata, Mayumi Kishida, Ayana Mori, Ryosuke Fujiwara, Akihiko Kondo, Yutaro Mori, Shuhei Noda, Tsutomu Tanaka

  Aug. 2024, Biotechnology Journal

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Production of lignin monomeric alcohols and lipids from oil palm empty fruit bunch by a combination of alkaline nitrobenzene depolymerization and Lipomyces starkeyi bioconversion

  Filemon Jalu Nusantara Putra, Prihardi Kahar, Akihiko Kondo, Chiaki Ogino

  Aug. 2024, Biochemical Engineering Journal

  [Refereed]

  Scientific journal


• Editing Streptomyces genome using target AID system fused with UGI‐degradation tag

  Pamella Apriliana, Prihardi Kahar, Norimasa Kashiwagi, Akihiko Kondo, Chiaki Ogino

  Jun. 2024, Engineering in Life Sciences

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Comparative responses of flocculating and nonflocculating yeasts to cell density and chemical stress in lactic acid fermentation.

  Radityo Pangestu, Prihardi Kahar, Chiaki Ogino, Akihiko Kondo

  While flocculation has demonstrated its efficacy in enhancing yeast robustness and ethanol production, its potential application for lactic acid fermentation remains largely unexplored. Our study examined the differences between flocculating and nonflocculating Saccharomyces cerevisiae strains in terms of their metabolic dynamics when incorporating an exogenous lactic acid pathway, across varying cell densities and in the presence of lignocellulose-derived byproducts. Comparative gene expression profiles revealed that cultivating a nonflocculant strain at higher cell density yielded a substantial upregulation of genes associated with glycolysis, energy metabolism, and other key pathways, resulting in elevated levels of fermentation products. Meanwhile, the flocculating strain displayed an inherent ability to sustain high glycolytic activity regardless of the cell density. Moreover, our investigation revealed a significant reduction in glycolytic activity under chemical stress, potentially attributable to diminished ATP supply during the energy investment phase. Conversely, the formation of flocs in the flocculating strain conferred protection against toxic chemicals present in the medium, fostering more stable lactic acid production levels. Additionally, the distinct flocculation traits observed between the two examined strains may be attributed to variations in the nucleotide sequences of the flocculin genes and their regulators. This study uncovers the potential of flocculation for enhanced lactic acid production in yeast, offering insights into metabolic mechanisms and potential gene targets for strain improvement.

  Apr. 2024, Yeast (Chichester, England), 41(4) (4), 192 - 206, English, International magazine

  Scientific journal


• Construction of a versatile in vitro cultivation screening platform using human oral microbiota.

  Kengo Sasaki, Yasunobu Takeshima, Ayami Fujino, Junya Yamashita, Akira Kimoto, Daisuke Sasaki, Akihiko Kondo, Masaya Akashi, Ryo Okumura

  We developed a simulation model of human oral microbiota using Bio Palette oral medium (BPOM) containing 0.02% glucose and lower bacterial nitrogen sources, derived from saliva and dental plaque. By decreasing the concentration of Gifu anaerobic medium (GAM) from 30 to 10 g L-1 , we observed increased ratios of target pathogenic genera, Porphyromonas and Fusobacterium from 0.5% and 1.7% to 1.2% and 3.5%, respectively, in the biofilm on hydroxyapatite (HA) discs. BPOM exhibited the higher ratios of Porphyromonas and Fusobacterium, and amplicon sequence variant number on HA, compared with GAM, modified GAM and basal medium mucin. Mixing glycerol stocks of BPOM culture solutions from four human subjects resulted in comparable ratios of these bacteria to the original saliva. In this simulation model, sitafloxacin showed higher inhibitory effects on P. gingivalis than minocycline hydrochloride at a low dosage of 0.1 μg mL-1 . Probiotics such as Streptococcus salivarius and Limosilactobacillus fermentum also showed significant decreases in Porphyromonas and Fusobacterium ratios on HA, respectively. Overall, the study suggests that BPOM with low carbon and nutrients could be a versatile platform for assessing the efficacy of antibiotics and live biotherapeutics in treating oral diseases caused by Porphyromonas and Fusobacterium.

  Apr. 2024, Environmental microbiology reports, 16(2) (2), e13243, English, International magazine

  Scientific journal


• Glycogen deficiency enhances carbon partitioning into glutamate for an alternative extracellular metabolic sink in cyanobacteria.

  Yuichi Kato, Ryota Hidese, Mami Matsuda, Ryudo Ohbayashi, Hiroki Ashida, Akihiko Kondo, Tomohisa Hasunuma

  Glycogen serves as a metabolic sink in cyanobacteria. Glycogen deficiency causes the extracellular release of distinctive metabolites such as pyruvate and 2-oxoglutarate upon nitrogen depletion; however, the mechanism has not been fully elucidated. This study aimed to elucidate the mechanism of carbon partitioning in glycogen-deficient cyanobacteria. Extracellular and intracellular metabolites in a glycogen-deficient ΔglgC mutant of Synechococcus elongatus PCC 7942 were comprehensively analyzed. In the presence of a nitrogen source, the ΔglgC mutant released extracellular glutamate rather than pyruvate and 2-oxoglutarate, whereas its intracellular glutamate level was lower than that in the wild-type strain. The de novo synthesis of glutamate increased in the ΔglgC mutant, suggesting that glycogen deficiency enhanced carbon partitioning into glutamate and extracellular excretion through an unidentified transport system. This study proposes a model in which glutamate serves as the prime extracellular metabolic sink alternative to glycogen when nitrogen is available.

  Feb. 2024, Communications biology, 7(1) (1), 233 - 233, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• High-level phenol bioproduction by engineered Pichia pastoris in glycerol fed-batch fermentation using an efficient pertraction system

  Ryota Kumokita, Takahiro Bamba, Hisashi Yasueda, Ayato Tsukida, Keizo Nakagawa, Tooru Kitagawa, Tomohisa Yoshioka, Hideto Matsuyama, Yasuhito Yamamoto, Satoshi Maruyama, Takahiro Hayashi, Akihiko Kondo, Tomohisa Hasunuma

  Elsevier BV, Feb. 2024, Bioresource Technology, 393, 130144 - 130144

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Engineering robust yeast for neutralizing-agent-free production of lactic acid from sugarcane bagasse hydrolysate

  Radityo Pangestu, Prihardi Kahar, Lutfi Kholida, Urip Perwitasari, Ahmad Thontowi, Fahrurrozi Fahrurrozi, Puspita Lisdiyanti, Yopi Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  Jan. 2024

  [Refereed]


• Metabolomics-based development of bioproduction processes toward industrial-scale production.

  Kenya Tanaka, Takahiro Bamba, Akihiko Kondo, Tomohisa Hasunuma

  Microbial biomanufacturing offers a promising, environment-friendly platform for next-generation chemical production. However, its limited industrial implementation is attributed to the slow production rates of target compounds and the time-intensive engineering of high-yield strains. This review highlights how metabolomics expedites bioproduction development, as demonstrated through case studies of its integration into microbial strain engineering, culture optimization, and model construction. The Design-Build-Test-Learn (DBTL) cycle serves as a standard workflow for strain engineering. Process development, including the optimization of culture conditions and scale-up, is crucial for industrial production. In silico models facilitate the development of strains and processes. Metabolomics is a powerful driver of the DBTL framework, process development, and model construction.

  Dec. 2023, Current opinion in biotechnology, 85, 103057 - 103057, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• ppGpp accumulation reduces the expression of the global nitrogen homeostasis-modulating NtcA regulon by affecting 2-oxoglutarate levels

  Ryota Hidese, Ryudo Ohbayashi, Yuichi Kato, Mami Matsuda, Kan Tanaka, Sousuke Imamura, Hiroki Ashida, Akihiko Kondo, Tomohisa Hasunuma

  Abstract The cyanobacterium Synechococcus elongatus PCC 7942 accumulates alarmone guanosine tetraphosphate (ppGpp) under stress conditions, such as darkness. A previous study observed that artificial ppGpp accumulation under photosynthetic conditions led to the downregulation of genes involved in the nitrogen assimilation system, which is activated by the global nitrogen regulator NtcA, suggesting that ppGpp regulates NtcA activity. However, the details of this mechanism have not been elucidated. Here, we investigate the metabolic responses associated with ppGpp accumulation by heterologous expression of the ppGpp synthetase RelQ. The pool size of 2-oxoglutarate (2-OG), which activates NtcA, is significantly decreased upon ppGpp accumulation. De novo ¹³C-labeled CO₂ assimilation into the Calvin-Benson-Bassham cycle and glycolytic intermediates continues irrespective of ppGpp accumulation, whereas the labeling of 2-OG is significantly decreased under ppGpp accumulation. The low 2-OG levels in the RelQ overexpression cells could be because of the inhibition of metabolic enzymes, including aconitase, which are responsible for 2-OG biosynthesis. We propose a metabolic rearrangement by ppGpp accumulation, which negatively regulates 2-OG levels to maintain carbon and nitrogen balance.

  Springer Science and Business Media LLC, Dec. 2023, Communications Biology, 6(1) (1)

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The bioconversion of lignin derivative aldehydes into high-value aromatic alcohols and lipids via Lipomyces starkeyi

  Filemon Jalu Nusantara Putra, Prihardi Kahar, Akihiko Kondo, Chiaki Ogino

  Nov. 2023, Biochemical Engineering Journal

  [Refereed]

  Scientific journal


• Minimized dark cataplerosis of the Calvin cycle ensures prompt photosynthetic initiation in cyanobacteria

  Kenya Tanaka, Akihiko Kondo, Tomohisa Hasunuma

  Oct. 2023


• Improving methanol assimilation in Yarrowia lipolytica via systematic metabolic engineering combined with compartmentalization

  Akihiko Kondo

  Oct. 2023, Green Chemistry, 25(1) (1), 183 - 195, English

  [Refereed]

  Scientific journal


• Sulfated and non-sulfated chondroitin affect the composition and metabolism of human colonic microbiota simulated in an in vitro fermentation system

  Kentaro Inokuma, Daisuke Sasaki, Kaoru Kurata, Megumi Ichikawa, Yuya Otsuka, Akihiko Kondo

  Abstract Chondroitin sulfate (CS) is a family of glycosaminoglycans and have a wide range of applications in dietary supplements and pharmaceutical drugs. In this study, we evaluated the effects of several types of CS, differing in their sulfated positions, on the human colonic microbiota and their metabolites. CS (CSA, CSC, and CSE) and non-sulfated chondroitin (CH) were added into an in vitro human colonic microbiota model with fecal samples from 10 healthy individuals. CS addition showed a tendency to increase the relative abundance of Bacteroides, Eubacterium, and Faecalibacterium, and CSC and CSE addition significantly increased the total number of eubacteria in the culture of the Kobe University Human Intestinal Microbiota Model. CSE addition also resulted in a significant increase in short-chain fatty acid (SCFA) levels. Furthermore, addition with CSC and CSE increased the levels of a wide range of metabolites including lysine, ornithine, and Ile-Pro-Pro, which could have beneficial effects on the host. However, significant increases in the total number of eubacteria, relative abundance of Bacteroides, and SCFA levels were also observed after addition with CH, and the trends in the effects of CH addition on metabolite concentrations were identical to those of CSC and CSE addition. These results provide novel insight into the contribution of the colonic microbiota to the beneficial effects of dietary CS.

  Springer Science and Business Media LLC, Jul. 2023, Scientific Reports, 13(1) (1), 12313, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Flocculation influences the response to increased cell density and chemical stresses during lactic acid fermentation in yeast

  Chiaki Ogino, Radityo Pangestu, Prihardi Kahar, Akihiko Kondo

  Jul. 2023


• Effects of partially hydrolyzed guar gums of different molecular weights on a human intestinal in vitro fermentation model.

  Daisuke Sasaki, Kengo Sasaki, Aya Abe, Makoto Ozeki, Akihiko Kondo

  Partially hydrolyzed guar gums (PHGGs) are prebiotic soluble dietary fibers. High molecular-weight PHGGs have rapid fermentation and high short-chain fatty acid (SCFA) productivity rates, compared to low molecular-weight PHGGs. Therefore, low molecular-weight PHGGs may have less pronounced prebiotic effects than high molecular-weight PHGGs. However, the effects of PHGGs of different molecular weights on the human intestinal microbiota, as well as their fermentation ability and prebiotic effects, have not been investigated. The aim of this study was to evaluate the effects of two PHGGs of different molecular weights, Sunfiber-R (SF-R; 20 kDa) and Sunfiber-V (SF-V; 5 kDa), on human colonic microbiota and SCFA production. A human intestinal in vitro fermentation model was operated by fecal samples with and without the PHGGs. The addition of 0.2% SF-R or SF-V increased the relative abundance of Bacteroides spp., especially that of Bacteroides uniformis. This increase corresponded to a significant (p = 0.030) and non-significant (p = 0.073) increase in propionate production in response to SF-R and SF-V addition, respectively. Both fibers increased the relative abundance of Faecalibacterium and stimulated an increase in the abundance of unclassified Lachnospiraceae and Bifidobacterium. In conclusion, the low molecular-weight PHGG exerted prebiotic effects on human colonic microbiota to increase SCFA production and bacteria that are beneficial to human health in a manner similar to that of the high molecular-weight forms of PHGG.

  Jul. 2023, Journal of bioscience and bioengineering, 136(1) (1), 67 - 73, English, Domestic magazine

  Scientific journal


• Hydroxybenzoic Acid Production Using Metabolically Engineered Corynebacterium glutamicum

  Misa Doke, Mayumi Kishida, Yuuki Hirata, Mariko Nakano, Mayo Horita, Daisuke Nonaka, Yutaro Mori, Ryosuke Fujiwara, Akihiko Kondo, Shuhei Noda, Tsutomu Tanaka

  Sciscan Publishing Limited, Jul. 2023, Synthetic Biology and Engineering, 1(2) (2), 10012 - 9

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Enhanced production of itaconic acid from enzymatic hydrolysate of lignocellulosic biomass by recombinant Corynebacteriumglutamicum.

  Dao Duy Hanh, Taghreed Elkasaby, Hideo Kawaguchi, Yota Tsuge, Chiaki Ogino, Akihiko Kondo

  Itaconic acid (IA) is a value-added chemical currently produced by Aspergillus terreus from edible glucose and starch but not from inedible lignocellulosic biomass owing to the high sensitivity to fermentation inhibitors present in the hydrolysate of lignocellulosic biomass. To produce IA from lignocellulosic biomass, a gram-positive bacterium, Corynebacterium glutamicum, with a high tolerance to fermentation inhibitors was metabolically engineered to express a fusion protein composed of cis-aconitate decarboxylase from A. terreus responsible for IA formation from cis-aconitate and a maltose-binding protein (malE) from Escherichia coli. The codon-optimized cadA_malE gene was expressed in C. glutamicum ATCC 13032, and the resulting recombinant strain produced IA from glucose. IA concentration increased 4.7-fold by the deletion of the ldh gene encoding lactate dehydrogenase. With the Δldh strain HKC2029, an 18-fold higher IA production was observed from enzymatic hydrolysate of kraft pulp as a model lignocellulosic biomass than from glucose (6.15 and 0.34 g/L, respectively). The enzymatic hydrolysate of kraft pulp contained various potential fermentation inhibitors involved in furan aldehydes, benzaldehydes, benzoic acids, cinnamic acid derivatives, and aliphatic acid. Whereas cinnamic acid derivatives severely inhibited IA production, furan aldehydes, benzoic acids, and aliphatic acid improved IA production at low concentrations. The present study suggests that lignocellulosic hydrolysate contains various potential fermentation inhibitors; however, some of them can serve as enhancers for microbial fermentation likely due to the changing of redox balance in the cell.

  Jul. 2023, Journal of bioscience and bioengineering, 136(1) (1), 7 - 12, English, Domestic magazine

  Scientific journal


• Circular cell culture for sustainable food production using recombinant lactate-assimilating cyanobacteria that supplies pyruvate and amino acids

  Yuji Haraguchi, Yuichi Kato, Kosuke Inabe, Akihiko Kondo, Tomohisa Hasunuma, Tatsuya Shimizu

  Springer Science and Business Media LLC, Jun. 2023, Archives of Microbiology, 205(7) (7), English

  [Refereed]

  Scientific journal


• Effect of different metabolic pathways on itaconic acid production in engineered Corynebacterium glutamicum.

  Taghreed Elkasaby, Dao Duy Hanh, Hideo Kawaguchi, Akihiko Kondo, Chiaki Ogino

  Itaconic acid (IA), a C5-dicarboxylic acid, is a potential bio-based building block for the polymer industry. There are three pathways for IA production from natural IA producers; however, most of the engineered strains were used for IA production by heterologous expression of cis-aconitate decarboxylase gene (cadA) from Aspergillus terreus. In this study, IA was produced by an engineered Corynebacterium glutamicum ATCC 13032 expressing two different types of genes from two distinct pathways. The first involves the mammalian immunoresponsive gene1 (Irg1) derived from Mus musculus. The second (termed here the trans-pathway) involves two genes from the natural IA producer Ustilago maydis which are aconitate-delta-isomerase (Adi1) and trans-aconitate decarboxylase (Tad1) genes. The constructed strains developing the two distinct IA production pathways: C. glutamicum ATCC 13032 pCH-Irg1opt and C. glutamicum ATCC 13032 pCH-Tad1optadi1opt were used for production of IA from different carbon sources. The results reflect the possibility for IA production from C. glutamicum expressing the trans-pathway (Adi1/Tad1 genes) and cis-pathway (Irg1 gene) other than the well-known cis-pathway that depends mainly on cadA gene from A. terreus. The developed strain expressing trans-pathway from U. maydis; however, proved to be better at IA production with high titers of 12.25, 11.34, and 11.02 g/L, and a molar yield of 0.22, 0.42, and 0.43 mol/mol from glucose, maltose, and sucrose, respectively, via fed-batch fermentation. The present study suggests that trans-pathway is better than cis-pathway for IA production in engineered C. glutamicum.

  Jun. 2023, Journal of bioscience and bioengineering, English, Domestic magazine

  Scientific journal


• l-Lactate treatment by photosynthetic cyanobacteria expressing heterogeneous l-lactate dehydrogenase

  Yuichi Kato, Kosuke Inabe, Yuji Haraguchi, Tatsuya Shimizu, Akihiko Kondo, Tomohisa Hasunuma

  Abstract l-Lactate is a major waste compound in cultured animal cells. To develop a sustainable animal cell culture system, we aimed to study the consumption of l-lactate using a photosynthetic microorganism. As genes involved in l-lactate utilization were not found in most cyanobacteria and microalgae, we introduced the NAD-independent l-lactate dehydrogenase gene from Escherichia coli (lldD) into Synechococcus sp. PCC 7002. The lldD-expressing strain consumed l-lactate added to basal medium. This consumption was accelerated by expression of a lactate permease gene from E. coli (lldP) and an increase in culture temperature. Intracellular levels of acetyl-CoA, citrate, 2-oxoglutarate, succinate, and malate, and extracellular levels of 2-oxoglutarate, succinate, and malate, increased during l-lactate utilization, suggesting that the metabolic flux from l-lactate was distributed toward the tricarboxylic acid cycle. This study provides a perspective on l-lactate treatment by photosynthetic microorganisms, which would increase the feasibility of animal cell culture industries.

  Springer Science and Business Media LLC, May 2023, Scientific Reports, 13(1) (1), English

  [Refereed]

  Scientific journal


• Metabolomic analysis of the effect of nitrogen on fucoxanthin synthesis by the haptophyte Pavlova gyrans

  Erina Yoshida, Yuichi Kato, Akihiko Kanamoto, Akihiko Kondo, Tomohisa Hasunuma

  Elsevier BV, May 2023, Algal Research, 72, 103144 - 103144, English

  [Refereed]

  Scientific journal


• Caffeic acid production from glucose using metabolically engineered Escherichia coli

  Akihiko Kondo

  Mar. 2023, Enzyme and Microbial Technology, 164, 110193 - 110193, English

  Scientific journal


• Enhanced production of 3,4‐dihydroxybutyrate from xylose by engineered yeast via xylonate re‐assimilation under alkaline condition

  Takahiro Yukawa, Takahiro Bamba, Mami Matsuda, Takanobu Yoshida, Kentaro Inokuma, Jungyeon Kim, Jae Won Lee, Yong‐Su Jin, Akihiko Kondo, Tomohisa Hasunuma

  Wiley, Feb. 2023, Biotechnology and Bioengineering, 120(2) (2), 511 - 523, English

  [Refereed]

  Scientific journal


• Pretreatment with dilute maleic acid enhances the enzymatic digestibility of sugarcane bagasse and oil palm empty fruit bunch fiber

  Lucky Risanto, Deddy Triyono Nugroho Adi, Triyani Fajriutami, Hiroshi Teramura, Widya Fatriasari, Euis Hermiati, Prihardi Kahar, Akihiko Kondo, Chiaki Ogino

  Lignocellulose is resistant to degradation and requires pretreatment before hydrolytic enzymes can release fermentable sugars. Sulfuric acid has been widely used for biomass pretreatment, but high amount of degradation products usually occurred when using this method. To enhance accessibility to cellulose, we studied the performances of several dilute organic acid pretreatments of sugarcane bagasse and oil palm empty fruit bunch fiber. The results revealed that pretreatment with maleic acid yields the highest xylose and glucose release among other organic acids. The effects of concentration, duration of heating and heating temperature were further studied. Dilute maleic acid 1% (w/w) pretreatment at 180 °C was the key to its viability as a substitute for sulfuric acid. Moreover, maleic acid did not seem to highly promote the formation of either furfural or 5-HMF in the liquid hydrolysate after pretreatment.

  Elsevier BV, Feb. 2023, Bioresource Technology, 369, 128382 - 128382, English, International magazine

  [Refereed]

  Scientific journal


• A multi-kingdom genetic barcoding system for precise target clone isolation

  Soh Ishiguro, Kana Ishida, Rina C. Sakata, Hideto Mori, Mamoru Takana, Samuel King, Omar Bashth, Minori Ichiraku, Nanami Masuyama, Ren Takimoto, Yusuke Kijima, Arman Adel, Hiromi Toyoshima, Motoaki Seki, Ju Hee Oh, Anne-Sophie Archambault, Keiji Nishida, Akihiko Kondo, Satoru Kuhara, Hiroyuki Aburatani, Ramon I. Klein Geltink, Yasuhiro Takashima, Nika Shakiba, Nozomu Yachie

  Clonal heterogeneity underlies diverse biological processes, including cancer progression, cell differentiation, and microbial evolution. Cell tagging strategies with DNA barcodes have recently enabled analysis of clone size dynamics and clone-restricted transcriptomic landscapes of heterogeneous populations. However, isolating a target clone that displays a specific phenotype from a complex population remains challenging. Here, we present a new multi-kingdom genetic barcoding system, CloneSelect, in which a target cell clone can be triggered to express a reporter gene for isolation through barcode-specific CRISPR base editing. In CloneSelect, cells are first barcoded and propagated so their subpopulation can be subjected to a given experiment. A clone that shows a phenotype or genotype of interest at a given time can then be isolated from the initial or subsequent cell pools stored throughout the experimental timecourse. This novel CRISPR-barcode genetics platform provides many new ways of analyzing and manipulating mammalian, yeast, and bacterial systems. Teaser A multi-kingdom CRISPR-activatable barcoding system enables the precise isolation of target barcode-labeled clones from a complex cell population.

  Cold Spring Harbor Laboratory, Jan. 2023


• Investigation of two metabolic engineering approaches for (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis

  Nunthaphan Vikromvarasiri, Shuhei Noda, Tomokazu Shirai, Akihiko Kondo

  Abstract Background Flux Balance Analysis (FBA) is a well-known bioinformatics tool for metabolic engineering design. Previously, we have successfully used single-level FBA to design metabolic fluxes in Bacillus subtilis to enhance (R,R)-2,3-butanediol (2,3-BD) production from glycerol. OptKnock is another powerful technique for devising gene deletion strategies to maximize microbial growth coupling with improved biochemical production. It has never been used in B. subtilis. In this study, we aimed to compare the use of single-level FBA and OptKnock for designing enhanced 2,3-BD production from glycerol in B. subtilis. Results Single-level FBA and OptKnock were used to design metabolic engineering approaches for B. subtilis to enhance 2,3-BD production from glycerol. Single-level FBA indicated that deletion of ackA, pta, lctE, and mmgA would improve the production of 2,3-BD from glycerol, while OptKnock simulation suggested the deletion of ackA, pta, mmgA, and zwf. Consequently, strains LM01 (single-level FBA-based) and MZ02 (OptKnock-based) were constructed, and their capacity to produce 2,3-BD from glycerol was investigated. The deletion of multiple genes did not negatively affect strain growth and glycerol utilization. The highest 2,3-BD production was detected in strain LM01. Strain MZ02 produced 2,3-BD at a similar level as the wild type, indicating that the OptKnock prediction was erroneous. Two-step FBA was performed to examine the reason for the erroneous OptKnock prediction. Interestingly, we newly found that zwf gene deletion in strain MZ02 improved lactate production, which has never been reported to date. The predictions of single-level FBA for strain MZ02 were in line with experimental findings. Conclusions We showed that single-level FBA is an effective approach for metabolic design and manipulation to enhance 2,3-BD production from glycerol in B. subtilis. Further, while this approach predicted the phenotypes of generated strains with high precision, OptKnock prediction was not accurate. We suggest that OptKnock modelling predictions be evaluated by using single-level FBA to ensure the accuracy of metabolic pathway design. Furthermore, the zwf gene knockout resulted in the change of metabolic fluxes to enhance the lactate productivity.

  Springer Science and Business Media LLC, Jan. 2023, Journal of Biological Engineering, 17(1) (1)

  Scientific journal


• Direct production of 4‐hydroxybenzoic acid from cellulose using cellulase‐displaying Pichia pastoris

  Kentaro Inokuma, Shunya Miyamoto, Kohei Morinaga, Yuma Kobayashi, Ryota Kumokita, Takahiro Bamba, Yoichiro Ito, Akihiko Kondo, Tomohisa Hasunuma

  Wiley, Jan. 2023, Biotechnology and Bioengineering, 120(4) (4), 1097 - 1107, English

  [Refereed]

  Scientific journal


• Production and Recovery of Ectoine: A Review of Current State and Future Prospects

  Akihiko Kondo

  2023, Processes

  Scientific journal


• Periictal water drinking revisited: Occurrence and lateralizing value in surgically confirmed patients with focal epilepsy

  Akihiko Kondo

  2023, Epilepsia Open

  Scientific journal


• A Single-center Analysis of Three Japanese Patients with Mahjong-related Seizures

  Akihiko Kondo

  2023, Internal Medicine

  Scientific journal


• Mesial temporal lobe epilepsy with amygdalar hamartoma-like lesion: Is it a distinct syndrome?

  Akihiko Kondo

  2023, Epilepsy Research

  Scientific journal


• Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant Escherichia coli

  Hideo Kawaguchi, Shunsuke Masuo, Keiko Wakai, Naoki Takaya, Tomohisa Hasunma, Tatsuo Kaneko, Satoshi Okada, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

  4-Aminophenylalanine as a diamine monomer to synthesize unique biopolyimides was produced from lignocellulosic biomass by microbial fermentation. Fermentation inhibitors present in lignocellulosic hydrolysate of sorghum bagasse were identified.

  Royal Society of Chemistry (RSC), 2023, RSC Sustainability

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Enhanced growth and lipid productivity by living Chlorella sorokiniana immobilized in Ca-alginate beads

  Akihiko Kondo

  Jan. 2023, Journal of Physics: Energy, 5(1) (1), 014019 - 014019, English

  [Refereed]

  Scientific journal


• Online SFE-SFC-MS/MS colony screening: A high-throughput approach for optimizing (-)-limonene production

  Akihiko Kondo

  Jan. 2023, Journal of Chromatography B, 1215, 123588 - 123588, English

  [Refereed]

  Scientific journal


• Dark accumulation of downstream glycolytic intermediates initiates robust photosynthesis in cyanobacteria

  Kenya Tanaka, Tomokazu Shirai, Christopher J Vavricka, Mami Matsuda, Akihiko Kondo, Tomohisa Hasunuma

  Abstract Photosynthesis must maintain stability and robustness throughout fluctuating natural environments. In cyanobacteria, dark-to-light transition leads to drastic metabolic changes from dark respiratory metabolism to CO2 fixation through the Calvin–Benson–Bassham (CBB) cycle using energy and redox equivalents provided by photosynthetic electron transfer. Previous studies have shown that catabolic metabolism supports the smooth transition into CBB cycle metabolism. However, metabolic mechanisms for robust initiation of photosynthesis are poorly understood due to lack of dynamic metabolic characterizations of dark-to-light transitions. Here, we show rapid dynamic changes (on a time scale of seconds) in absolute metabolite concentrations and 13C tracer incorporation after strong or weak light irradiation in the cyanobacterium Synechocystis sp. PCC 6803. Integration of this data enabled estimation of time-resolved nonstationary metabolic flux underlying CBB cycle activation. This dynamic metabolic analysis indicated that downstream glycolytic intermediates, including phosphoglycerate and phosphoenolpyruvate, accumulate under dark conditions as major substrates for initial CO2 fixation. Compared with wild-type Synechocystis, significant decreases in the initial oxygen evolution rate were observed in 12 h dark preincubated mutants deficient in glycogen degradation or oxidative pentose phosphate pathways. Accordingly, the degree of decrease in the initial oxygen evolution rate was proportional to the accumulated pool size of glycolytic intermediates. These observations indicate that the accumulation of glycolytic intermediates is essential for efficient metabolism switching under fluctuating light environments.

  Oxford University Press (OUP), Dec. 2022, Plant Physiology, English

  [Refereed]

  Scientific journal


• Simple and Rapid Non-ribosomal Peptide Synthetase Gene Assembly Using the SEAM–OGAB Method

  Varada Jagadeesh, Takanobu Yoshida, Misugi Uraji, Nobuyuki Okahashi, Fumio Matsuda, Christopher J. Vavricka, Kenji Tsuge, Akihiko Kondo

  American Chemical Society (ACS), Dec. 2022, ACS Synthetic Biology, 12(1) (1), 305 - 318, English

  [Refereed]

  Scientific journal


• Alginate immobilization as a strategy for improving succinate production during autofermentation using cyanobacteria Synechocystis sp. PCC 6803

  Akihiko Kondo

  Dec. 2022, Biochemical Engineering Journal, 188, 108681 - 108681, English

  [Refereed]

  Scientific journal


• Machine learning discovery of missing links that mediate alternative branches to plant alkaloids

  Christopher J. Vavricka, Shunsuke Takahashi, Naoki Watanabe, Musashi Takenaka, Mami Matsuda, Takanobu Yoshida, Ryo Suzuki, Hiromasa Kiyota, Jianyong Li, Hiromichi Minami, Jun Ishii, Kenji Tsuge, Michihiro Araki, Akihiko Kondo, Tomohisa Hasunuma

  Abstract Engineering the microbial production of secondary metabolites is limited by the known reactions of correctly annotated enzymes. Therefore, the machine learning discovery of specialized enzymes offers great potential to expand the range of biosynthesis pathways. Benzylisoquinoline alkaloid production is a model example of metabolic engineering with potential to revolutionize the paradigm of sustainable biomanufacturing. Existing bacterial studies utilize a norlaudanosoline pathway, whereas plants contain a more stable norcoclaurine pathway, which is exploited in yeast. However, committed aromatic precursors are still produced using microbial enzymes that remain elusive in plants, and additional downstream missing links remain hidden within highly duplicated plant gene families. In the current study, machine learning is applied to predict and select plant missing link enzymes from homologous candidate sequences. Metabolomics-based characterization of the selected sequences reveals potential aromatic acetaldehyde synthases and phenylpyruvate decarboxylases in reconstructed plant gene-only benzylisoquinoline alkaloid pathways from tyrosine. Synergistic application of the aryl acetaldehyde producing enzymes results in enhanced benzylisoquinoline alkaloid production through hybrid norcoclaurine and norlaudanosoline pathways.

  Springer Science and Business Media LLC, Dec. 2022, Nature Communications, 13(1) (1), 1405 - 1405, English, International magazine

  Scientific journal


• Metabolic and Microbial Community Engineering for Four-Carbon Dicarboxylic Acid Production from CO2-Derived Glycogen in the Cyanobacterium Synechocystis sp. PCC6803.

  Ryota Hidese, Mami Matsuda, Mamiko Kajikawa, Takashi Osanai, Akihiko Kondo, Tomohisa Hasunuma

  The four-carbon (C4) dicarboxylic acids, fumarate, malate, and succinate, are the most valuable targets that must be exploited for CO2-based chemical production in the move to a sustainable low-carbon future. Cyanobacteria excrete high amounts of C4 dicarboxylic acids through glycogen fermentation in a dark anoxic environment. The enhancement of metabolic flux in the reductive TCA branch in the Cyanobacterium Synechocystis sp. PCC6803 is a key issue in the C4 dicarboxylic acid production. To improve metabolic flux through the anaplerotic pathway, we have created the recombinant strain PCCK, which expresses foreign ATP-forming phosphoenolpyruvate carboxykinase (PEPck) concurrent with intrinsic phosphoenolpyruvate carboxylase (Ppc) overexpression. Expression of PEPck concurrent with Ppc led to an increase in C4 dicarboxylic acids by autofermentation. Metabolome analysis revealed that PEPck contributed to an increase in carbon flux from hexose and pentose phosphates into the TCA reductive branch. To enhance the metabolic flux in the reductive TCA branch, we examined the effect of corn-steep liquor (CSL) as a nutritional supplement on C4 dicarboxylic acid production. Surprisingly, the addition of sterilized CSL enhanced the malate production in the PCCK strain. Thereafter, the malate and fumarate excreted by the PCCK strain are converted into succinate by the CSL-settling microorganisms. Finally, high-density cultivation of cells lacking the acetate kinase gene showed the highest production of malate and fumarate (3.2 and 2.4 g/L with sterilized CSL) and succinate (5.7 g/L with non-sterile CSL) after 72 h cultivation. The present microbial community engineering is useful for succinate production by one-pot fermentation under dark anoxic conditions.

  American Chemical Society (ACS), Nov. 2022, ACS synthetic biology, 11(12) (12), 4054 - 4064, English, International magazine

  [Refereed]

  Scientific journal


• Metabolic engineering of Schizosaccharomyces pombe for itaconic acid production

  Akihiko Kondo

  Nov. 2022, Journal of Biotechnology, 358, 111 - 117, English

  [Refereed]

  Scientific journal


• Development of a stable semi-continuous lipid production system of an oleaginous Chlamydomonas sp. mutant using multi-omics profiling

  Tomoki Oyama, Yuichi Kato, Ryota Hidese, Mami Matsuda, Minenosuke Matsutani, Satoru Watanabe, Akihiko Kondo, Tomohisa Hasunuma

  Abstract Background Microalgal lipid production has attracted global attention in next-generation biofuel research. Nitrogen starvation, which drastically suppresses cell growth, is a common and strong trigger for lipid accumulation in microalgae. We previously developed a mutant Chlamydomonas sp. KAC1801, which can accumulate lipids irrespective of the presence or absence of nitrates. This study aimed to develop a feasible strategy for stable and continuous lipid production through semi-continuous culture of KAC1801. Results KAC1801 continuously accumulated > 20% lipid throughout the subculture (five generations) when inoculated with a dry cell weight of 0.8–0.9 g L⁻¹ and cultured in a medium containing 18.7 mM nitrate, whereas the parent strain KOR1 accumulated only 9% lipid. Under these conditions, KAC1801 continuously produced biomass and consumed nitrates. Lipid productivity of 116.9 mg L⁻¹ day⁻¹ was achieved by semi-continuous cultivation of KAC1801, which was 2.3-fold higher than that of KOR1 (50.5 mg L⁻¹ day⁻¹). Metabolome and transcriptome analyses revealed a depression in photosynthesis and activation of nitrogen assimilation in KAC1801, which are the typical phenotypes of microalgae under nitrogen starvation. Conclusions By optimizing nitrate supply and cell density, a one-step cultivation system for Chlamydomonas sp. KAC1801 under nitrate-replete conditions was successfully developed. KAC1801 achieved a lipid productivity comparable to previously reported levels under nitrogen-limiting conditions. In the culture system of this study, metabolome and transcriptome analyses revealed a nitrogen starvation-like response in KAC1801.

  Springer Science and Business Media LLC, Sep. 2022, Biotechnology for Biofuels and Bioproducts, 15(1) (1), English

  [Refereed]

  Scientific journal


• Comprehensive Machine Learning Prediction of Extensive Enzymatic Reactions

  Naoki Watanabe, Masaki Yamamoto, Masahiro Murata, Christopher J. Vavricka, Chiaki Ogino, Akihiko Kondo, Michihiro Araki

  Sep. 2022, Journal of Physical Chemistry B, 126(36) (36), 6762 - 6770

  Scientific journal


• Comprehensive Machine Learning Prediction of Extensive Enzymatic Reactions.

  Naoki Watanabe, Masaki Yamamoto, Masahiro Murata, Christopher J Vavricka, Chiaki Ogino, Akihiko Kondo, Michihiro Araki

  New enzyme functions exist within the increasing number of unannotated protein sequences. Novel enzyme discovery is necessary to expand the pathways that can be accessed by metabolic engineering for the biosynthesis of functional compounds. Accordingly, various machine learning models have been developed to predict enzymatic reactions. However, the ability to predict unknown reactions that are not included in the training data has not been clarified. In order to cover uncertain and unknown reactions, a wider range of reaction types must be demonstrated by the models. Here, we establish 16 expanded enzymatic reaction prediction models developed using various machine learning algorithms, including deep neural network. Improvements in prediction performances over that of our previous study indicate that the updated methods are more effective for the prediction of enzymatic reactions. Overall, the deep neural network model trained with combined substrate-enzyme-product information exhibits the highest prediction accuracy with Macro F1 scores up to 0.966 and with robust prediction of unknown enzymatic reactions that are not included in the training data. This model can predict more extensive enzymatic reactions in comparison to previously reported models. This study will facilitate the discovery of new enzymes for the production of useful substances.

  Sep. 2022, The journal of physical chemistry. B, 126(36) (36), 6762 - 6770, English, International magazine

  Scientific journal


• Avoiding entry into intracellular protein degradation pathways by signal mutations increases protein secretion in Pichia pastoris

  Yoichiro Ito, Misa Ishigami, Noriko Hashiba, Yasuyuki Nakamura, Goro Terai, Tomohisa Hasunuma, Jun Ishii, Akihiko Kondo

  Wiley, Sep. 2022, Microbial Biotechnology, 15(9) (9), 2364 - 2378, English

  [Refereed]

  Scientific journal


• Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization.

  Radityo Pangestu, Prihardi Kahar, Lutfi Nia Kholida, Urip Perwitasari, Ahmad Thontowi, Fahrurrozi, Puspita Lisdiyanti, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  Acidic and chemical inhibitor stresses undermine efficient lactic acid bioproduction from lignocellulosic feedstock. Requisite coping treatments, such as detoxification and neutralizing agent supplementation, can be eliminated if a strong microbial host is employed in the process. Here, we exploited an originally robust yeast, Saccharomyces cerevisiae BTCC3, as a production platform for lactic acid. This wild-type strain exhibited a rapid cell growth in the presence of various chemical inhibitors compared to laboratory and industrial strains, namely BY4741 and Ethanol-red. Pathway engineering was performed on the strain by introducing an exogenous LDH gene after disrupting the PDC1 and PDC5 genes. Facilitated by this engineered strain, high cell density cultivation could generate lactic acid with productivity at 4.80 and 3.68 g L-1 h-1 under semi-neutralized and non-neutralized conditions, respectively. Those values were relatively higher compared to other studies. Cultivation using real lignocellulosic hydrolysate was conducted to assess the performance of this engineered strain. Non-neutralized fermentation using non-detoxified hydrolysate from sugarcane bagasse as a medium could produce lactic acid at 1.69 g L-1 h-1, which was competitive to the results from other reports that still included detoxification and neutralization steps in their experiments. This strategy could make the overall lactic acid bioproduction process simpler, greener, and more cost-efficient.

  Springer Science and Business Media LLC, Aug. 2022, Scientific reports, 12(1) (1), 13645 - 13645, English, International magazine

  Scientific journal


• Metabolic engineering of the L-serine biosynthetic pathway improves glutathione production in Saccharomyces cerevisiae.

  Jyumpei Kobayashi, Daisuke Sasaki, Kiyotaka Y Hara, Tomohisa Hasunuma, Akihiko Kondo

  BACKGROUND: Glutathione is a valuable tri-peptide that is industrially produced by fermentation using the yeast Saccharomyces cerevisiae, and is widely used in the pharmaceutical, food, and cosmetic industries. It has been reported that addition of L-serine (L-Ser) is effective at increasing the intracellular glutathione content because L-Ser is the common precursor of L-cysteine (L-Cys) and glycine (Gly) which are substrates for glutathione biosynthesis. Therefore, we tried to enhance the L-Ser biosynthetic pathway in S. cerevisiae for improved glutathione production. RESULTS: The volumetric glutathione production of recombinant strains individually overexpressing SER2, SER1, SER3, and SER33 involved in L-Ser biosynthesis at 48 h cultivation was increased 1.3, 1.4, 1.9, and 1.9-fold, respectively, compared with that of the host GCI strain, which overexpresses genes involved in glutathione biosynthesis. We further examined simultaneous overexpression of SHM2 and/or CYS4 genes involved in Gly and L-Cys biosynthesis, respectively, using recombinant GCI strain overexpressing SER3 and SER33 as hosts. As a result, GCI overexpressing SER3, SHM2, and CYS4 showed the highest volumetric glutathione production (64.0 ± 4.9 mg/L) at 48 h cultivation, and this value is about 2.5-fold higher than that of the control strain. CONCLUSIONS: This study first revealed that engineering of L-Ser and Gly biosynthetic pathway are useful strategies for fermentative glutathione production by S. cerevisiase.

  Springer Science and Business Media LLC, Aug. 2022, Microbial cell factories, 21(1) (1), 153 - 153, English, International magazine

  [Refereed]

  Scientific journal


• Evaluation of ionic liquids/salt aqueous biphasic flotation system on recovery of Kytococcus sedentarius TWHKC01 keratinase from crude feedstock

  Akihiko Kondo

  Aug. 2022, Journal of the Taiwan Institute of Chemical Engineers, 137, 104198 - 104198, English

  Scientific journal


• Plant Flavonoid Production in Bacteria and Yeasts

  Shota Isogai, Masahiro Tominaga, Akihiko Kondo, Jun Ishii

  Jul. 2022, Frontiers in Chemical Engineering, 4, 880694, English

  Scientific journal


• 3-Amino-4-hydroxybenzoic acid production from glucose and/or xylose via recombinant <i>Streptomyces lividans</i>

  Satoko Niimi-Nakamura, Hideo Kawaguchi, Kouji Uematsu, Hiroshi Teramura, Sachiko Nakamura-Tsuruta, Norimasa Kashiwagi, Yoshinori Sugai, Yohei Katsuyama, Yasuo Ohnishi, Chiaki Ogino, Akihiko Kondo

  The aromatic compound 3-amino-4-hydroxybenzoic acid (3,4-AHBA) can be employed as a raw material for high-performance industrial plastics. The aim of this study is to produce 3,4-AHBA via a recombinant Streptomyces lividans strain containing griI and griH genes derived from Streptomyces griseus using culture medium with glucose and/or xylose, which are the main components in lignocellulosic biomass. Production of 3,4-AHBA by the recombinant S. lividans strain was successful, and the productivity was affected by the kind of sugar used as an additional carbon source. Metabolic profiles revealed that L aspartate-4-semialdehyde (ASA), a precursor of 3,4-AHBA, and coenzyme NADPH were supplied in greater amounts in xylose medium than in glucose medium. Moreover, cultivation in TSB medium with a mixed sugar (glucose/xylose) was found to be effective for 3,4-AHBA production, and optimal conditions for efficient production were designed by changing the ratio of glucose to xylose. The best productivity of 2.70 g/L was achieved using a sugar mixture of 25 g/L glucose and 25 g/L xylose, which was 1.5 times higher than the result using 50 g/L glucose alone. These results suggest that Streptomyces is a suitable candidate platform for 3,4-AHBA production from lignocellulosic biomass-derived sugars under appropriate culture conditions.

  Microbiology Research Foundation, Jul. 2022, The Journal of General and Applied Microbiology, 68(2) (2), 109 - 116, English, Domestic magazine

  Scientific journal


• Enhanced squalene production by modulation of pathways consuming squalene and its precursor

  Masahiro Tominaga, Keita Miyazaki, Shoko Hataya, Yasumasa Mitsui, Shuji Kuroda, Akihiko Kondo, Jun Ishii

  Fermentative production of squalene in yeast as an alternative approach to extracting squalene from sharks or plants has attracted significant interest. However, squalene accumulation is limited due to its inevitable high-flux allocation toward ergosterol synthesis. In this study, we described expression control of squalene monooxygenase (Erg1p), the first-step enzyme of ergosterol synthesis from squalene, to significantly reduce squalene loss. We replaced the ERG1 promoter (PERG1) with three natural yeast promoters with different activities (PPCL2, PHCM1, and PTHI2). ERG1 controlled by PTHI2 showed 20 times higher squalene production compared with the wild-type strain, whereas the other two strains exhibited no significant difference. By combining the overexpression of rate-limiting enzyme and the deletion of non-essential competing pathway gene, the yeast Saccharomyces cerevisiae produced up to 379 mg/L of squalene.

  Elsevier BV, Jul. 2022, Journal of Bioscience and Bioengineering, 134(1) (1), 1 - 6, English, Domestic magazine

  [Refereed]

  Scientific journal


• The flocculant Saccharomyces cerevisiae strain gains robustness via alteration of the cell wall hydrophobicity

  Prihardi Kahar, Akiho Itomi, Hikari Tsuboi, Miki Ishizaki, Misa Yasuda, Chie Kihira, Hiromi Otsuka, Nurlina binti Azmi, Hana Matsumoto, Chiaki Ogino, Akihiko Kondo

  When lignocellulosic biomass is utilized as a fermentative substrate to produce biochemicals, the existence of a yeast strain resistant to inhibitory chemical compounds (ICCs) released from the biomass becomes critical. To achieve the purpose, in this study, Saccharomyces yeast strains from a NBRC yeast culture collection were used for exploration and evaluated in two different media containing ICCs that mimic one another but resemble the hydrolysate of real biomass. Among them, S. cerevisiae F118 strain shows robustness upon the fermentation with unique flocculation trait that was strongly responsive to ICC stress. When this strain was cultured in the presence of ICCs, its cell wall hydrophobicity increased dramatically, and reduced significantly when the ICCs were depleted, demonstrating that cell-surface hydrophobicity can also act as an adaptive response to the ICCs. Cells from the strain with the highest cell-wall hydrophobicity displayed progressively stronger flocculation, indicating that the F118 strain is having unique robustness under ICC stress. Gene expression perturbation analysis revealed that mot3 gene encoding regulatory Mot3p from the F118 strain was expressed in response to the concentration of ICCs. This gene was found to control expression of ygp1 gene that encoding Ygp1p, one of cell wall proteins. Deep sequencing analysis revealed that the Mot3p of the F118 strain features a unique insertion and deletion of nucleotides that encode glutamine or asparagine residues, particularly in N-terminal domain, as determined by comparison to the Mot3p sequence from the S288c strain, which was employed as a control strain. Furthermore, the cell wall hydrophobicity of the S288c strain was greatly enhanced and became ICC-responsive after gene swapping with the mot3 gene from the F118 strain. The gene-swapped S288c strain fermented 6-fold faster than the wild-type strain, producing 14.5 g/L of ethanol from 30 g/L of glucose consumed within 24 h in a medium containing the ICCs. These such modifications to Mot3p in unique locations in its sequence have a potential to change the expression of a gene involved in cell wall hydrophobicity and boosted the flocculation response to ICC stress, allowing for the acquisition of extraordinary robustness.

  Elsevier BV, Jul. 2022, Metabolic Engineering, 72, 82 - 96, English, International magazine

  Scientific journal


• G6P-capturing molecules in the periplasm of Escherichia coli accelerate the shikimate pathway

  Ryosuke Fujiwara, Mariko Nakano, Yuuki Hirata, Chisako Otomo, Daisuke Nonaka, Sakiya Kawada, Hikaru Nakazawa, Mitsuo Umetsu, Tomokazu Shirai, Shuhei Noda, Tsutomu Tanaka, Akihiko Kondo

  Escherichia coli, the most studied prokaryote, is an excellent host for producing valuable chemicals from renewable resources as it is easy to manipulate genetically. Since the periplasmic environment can be easily controlled externally, elucidating how the localization of specific proteins or small molecules in the periplasm affects metabolism may lead to bioproduction development using E. coli. We investigated metabolic changes and its mechanisms occurring when specific proteins are localized to the E. coli periplasm. We found that the periplasmic localization of β-glucosidase promoted the shikimate pathway involved in the synthesis of aromatic chemicals. The periplasmic localization of other proteins with an affinity for glucose-6-phosphate (G6P), such as inactivated mutants of Pgi, Zwf, and PhoA, similarly accelerated the shikimate pathway. Our results indicate that G6P is transported from the cytoplasm to the periplasm by the glucose transporter protein EIICBGlc, and then captured by β-glucosidase.

  Elsevier BV, Jul. 2022, Metabolic Engineering, 72, 68 - 81, English, International magazine

  Scientific journal


• Construction of an l-Tyrosine Chassis in Pichia pastoris Enhances Aromatic Secondary Metabolite Production from Glycerol

  Ryota Kumokita, Takahiro Bamba, Kentaro Inokuma, Takanobu Yoshida, Yoichiro Ito, Akihiko Kondo, Tomohisa Hasunuma

  American Chemical Society (ACS), Jun. 2022, ACS Synthetic Biology, 11(6) (6), 2098 - 2107, English

  Scientific journal


• A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella phaffii

  Akihiko Kondo

  Jun. 2022, Communications Biology, 5(1) (1), 561, English

  [Refereed]

  Scientific journal


• Integrated bioconversion process for biodiesel production utilizing waste from the palm oil industry

  Akihiko Kondo

  Jun. 2022, Journal of Environmental Chemical Engineering, 10(3) (3), 107550 - 107550, English

  [Refereed]

  Scientific journal


• In Silico Design Strategies for the Production of Target Chemical Compounds Using Iterative Single-Level Linear Programming Problems.

  Tomokazu Shirai, Akihiko Kondo

  The optimization of metabolic reaction modifications for the production of target compounds is a complex computational problem whose execution time increases exponentially with the number of metabolic reactions. Therefore, practical technologies are needed to identify reaction deletion combinations to minimize computing times and promote the production of target compounds by modifying intracellular metabolism. In this paper, a practical metabolic design technology named AERITH is proposed for high-throughput target compound production. This method can optimize the production of compounds of interest while maximizing cell growth. With this approach, an appropriate combination of metabolic reaction deletions can be identified by solving a simple linear programming problem. Using a standard CPU, the computation time could be as low as 1 min per compound, and the system can even handle large metabolic models. AERITH was implemented in MATLAB and is freely available for non-profit use.

  Apr. 2022, Biomolecules, 12(5) (5), English, International magazine

  Scientific journal


• Engineering of Synthetic Transcriptional Switches in Yeast

  Masahiro Tominaga, Akihiko Kondo, Jun Ishii

  Apr. 2022, Life, 12(4) (4), 557 - 557, English

  [Refereed]

  Scientific journal


• Accumulated 3-phosphoglycerate in darkness is a primary starting substrate for stepwise photosynthetic induction process in cyanobacteria

  Akihiko Kondo

  Cold Spring Harbor Laboratory, Apr. 2022, BioRxiv

  Scientific journal


• L‐Lactate oxidase‐mediated removal of L‐lactic acid derived from fermentation medium for the production of optically pure D‐lactic acid

  Kenji Okano, Yu Sato, Shnji Hama, Tsutomu Tanaka, Hideo Noda, Akihiko Kondo, Kohsuke Honda

  BACKGROUND: There has been an increasing demand for optically pure d-lactic and l-lactic acid for the production of stereocomplex-type polylactic acid. The d-lactic acid production from lignocellulosic biomass is important owing to its great abundance in nature. Corn steep liquor (CSL) is a cheap nitrogen source used for industrial fermentation, though it contains a significant amount of l-lactic acid, which decreases the optical purity of d-lactic acid produced. METHOD AND RESULTS: To remove l-lactic acid derived from the CSL-based medium, l-lactate oxidase (LoxL) from Enterococcus sp. NBRC 3427 was expressed in an engineered Lactiplantibacillus plantarum (formally called Lactobacillus plantarum) strain KOLP7, which exclusively produces d-lactic acid from both hexose and pentose sugars. When the resulting strain was applied for d-lactic acid fermentation from the mixed sugars consisting of the major constituent sugars of lignocellulose (35 g L-1 glucose, 10 g L-1 xylose, and 5 g L-1 arabinose) using the medium containing 10 g L-1 CSL, it completely removed l-lactic acid derived from CSL (0.52 g L-1 ) and produced 41.7 g L-1 of d-lactic acid. The l-lactic acid concentration was below the detection limit, and improvement in the optical purity of d-lactic acid was observed (from 98.2% to > 99.99%) by the overexpression of LoxL. CONCLUSION AND IMPLICATIONS: The LoxL-mediated consumption of l-lactic acid would enable the production of optically pure d-lactic acid in any medium contaminated by l-lactic acid.

  Wiley, Apr. 2022, Biotechnology Journal, 17(4) (4), 2100331 - 2100331, English, International magazine

  Scientific journal


• One-Step In Vivo Assembly of Multiple DNA Fragments and Genomic Integration in Komagataella phaffii

  Teruyuki Nishi, Yoichiro Ito, Yasuyuki Nakamura, Taiki Yamaji, Noriko Hashiba, Masaya Tamai, Yoshihiko Yasohara, Jun Ishii, Akihiko Kondo

  American Chemical Society (ACS), Feb. 2022, ACS Synthetic Biology, 11(2) (2), 644 - 654, English

  Scientific journal


• Manno-Oligosaccharide Production from Biomass Hydrolysis by Using Endo-1,4-beta-Mannanase (ManNj6-379) from Nonomuraea jabiensis ID06-379

  Shanti Ratnakomala, Prihardi Kahar, Norimasa Kashiwagi, JaeMin Lee, Motonori Kudou, Hana Matsumoto, Pamella Apriliana, Yopi Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

  Feb. 2022, PROCESSES, 10(2) (2), 269 - 269, English

  Scientific journal


• Enzyme display technology for lignocellulosic biomass valorization by yeast cell factories

  Akihiko Kondo

  Feb. 2022, Current Opinion in Green and Sustainable Chemistry, 33, 100584 - 100584, English

  Scientific journal


• Modification of tomato breeding traits and plant hormone signaling by Target-AID, the genome-editing system inducing efficient nucleotide substitution

  Sachiko Kashojiya, Yu Lu, Mariko Takayama, Hiroki Komatsu, Luyen Hieu Thi Minh, Keiji Nishida, Kenta Shirasawa, Kenji Miura, Satoko Nonaka, Jun-ichiro Masuda, Akihiko Kondo, Hiroshi Ezura, Tohru Ariizumi

  Abstract Target activation-induced cytidine deaminase (Target-AID), a novel CRISPR/Cas9-based genome-editing tool, confers the base-editing capability on the Cas9 genome-editing system. It involves the fusion of cytidine deaminase (CDA), which catalyzes cytidine (C) to uridine (U) substitutions, to the mutated nickase-type nCas9 or deactivated-type dCas9. To confirm and extend the applicability of the Target-AID genome-editing system in tomatoes (Solanum lycopersicum L.), we transformed the model tomato cultivar “Micro-Tom” and commercial tomato cultivars using this system by targeting SlDELLA, which encodes a negative regulator of the plant phytohormone gibberellic acid (GA) signaling pathway. We confirmed that the nucleotide substitutions were induced by the Target-AID system, and we isolated mutants showing high GA sensitivity in both “Micro-Tom” and the commercial cultivars. Moreover, by successfully applying this system to ETHYLENE RECEPTOR 1 (SlETR1) with single sgRNA targeting, double sgRNA targeting, as well as dual-targeting of both SlETR1 and SlETR2 with a single sgRNA, we demonstrated that the Target-AID genome-editing system is a promising tool for molecular breeding in tomato crops. This study highlights an important aspect of the scientific and agricultural potential of the combinatorial use of the Target-AID and other base-editing systems.

  Oxford University Press (OUP), Jan. 2022, Horticulture Research, 9, uhab004, English, International magazine

  Scientific journal


• Obligate aerobic, gram-positive, weak acid-fast, nonmotile bacilli, Tsukamurella tyrosinosolvens: Minireview of a rare opportunistic pathogen

  Akihiko Kondo

  2022, World Journal of Clinical Cases

  Scientific journal


• Hemorrhagic shock due to submucosal esophageal hematoma along with mallory-weiss syndrome: A case report

  Akihiko Kondo

  2022, World Journal of Clinical Cases

  Scientific journal


• Extractive fermentation of Kytococcus sedentarius TWHKC01 using the aqueous biphasic system for direct recovery of keratinase

  Akihiko Kondo

  2022, Journal of the Taiwan Institute of Chemical Engineers

  Scientific journal


• Engineered Nanogel Particles Enhance the PhotoautotrophicBiosynthesis of Polyhydroxyalkanoate in Marine PhotosyntheticBacteria

  Akihiko Kondo

  2022, ACS Sustainable Chemistry & Engineering

  Scientific journal


• Editorial: Engineering Corynebacterium glutamicum Chassis for Synthetic Biology, Biomanufacturing, and Bioremediation

  Akihiko Kondo

  2022, Frontiers in Bioengineering and Biotechnology

  Scientific journal


• Amebic liver abscess by Entamoeba histolytica

  Akihiko Kondo

  2022, World Journal of Clinical Cases

  Scientific journal


• Phenotypic Characterization of High Carotenoid Tomato Mutants Generated by the Target-AID Base-Editing Technology.

  Johan Hunziker, Keiji Nishida, Akihiko Kondo, Tohru Ariizumi, Hiroshi Ezura

  Our previous study demonstrated that Target-AID which is the modified CRISPR/Cas9 system enabling base-editing is an efficient tool for targeting multiple genes. Three genes, SlDDB1, SlDET1, and SlCYC-B, responsible for carotenoid accumulation were targeted, and allelic variations were previously obtained by Target-AID. In this research, we characterized the effect of new alleles on plant growth and fruit development, as well as carotenoid accumulation, individually in segregating backcross populations or combined in null self-segregant lines. Only lines carrying homozygous substitutions in the three targeted genes and the segregating backcross population of individual mutations were characterized, resulting in the isolation of two allelic versions for SlDDB1, one associated with SlDET1 and the last one with SlCYC-B. All edited lines showed variations in carotenoid accumulation, with an additive effect for each single mutation. These results suggest that Target-AID base-editing technology is an effective tool for creating new allelic variations in target genes to improve carotenoid accumulation in tomato.

  2022, Frontiers in plant science, 13, 848560 - 848560, English, International magazine

  Scientific journal


• Improved ethanol fermentation by promoter replacement of zinc responsive genes IPL1, PRP6 and RTC1 in Saccharomyces cerevisiae

  Akihiko Kondo

  Jan. 2022, Biochemical Engineering Journal, 178, 108274 - 108274

  Scientific journal


• Efficient preparation of soluble inducer for cellulase production and saccharification of corn stover using in-house generated crude enzymes

  Akihiko Kondo

  Jan. 2022, Biochemical Engineering Journal, 178, 108296 - 108296

  Scientific journal


• Metabolomics-based engineering for biofuel and bio-based chemical production in microalgae and cyanobacteria: A review

  Yuichi Kato, Kosuke Inabe, Ryota Hidese, Akihiko Kondo, Tomohisa Hasunuma

  Metabolomics, an essential tool in modern synthetic biology based on the design-build-test-learn platform, is useful for obtaining a detailed understanding of cellular metabolic mechanisms through comprehensive analyses of the metabolite pool size and its dynamic changes. Metabolomics is critical to the design of a rational metabolic engineering strategy by determining the rate-limiting reaction and assimilated carbon distribution in a biosynthetic pathway of interest. Microalgae and cyanobacteria are promising photosynthetic producers of biofuels and bio-based chemicals, with high potential for developing a bioeconomic society through bio-based carbon neutral manufacturing. Metabolomics technologies optimized for photosynthetic organisms have been developed and utilized in various microalgal and cyanobacterial species. This review provides a concise overview of recent achievements in photosynthetic metabolomics, emphasizing the importance of microalgal and cyanobacterial cell factories that satisfy industrial requirements.

  Elsevier BV, Jan. 2022, Bioresource Technology, 344(Pt A) (Pt A), 126196 - 126196, English, International magazine

  Scientific journal


• Resveratrol production of a recombinant Scheffersomyces stipitis strain from molasses

  Yuma Kobayashi, Kentaro Inokuma, Mami Matsuda, Akihiko Kondo, Tomohisa Hasunuma

  Elsevier BV, Jan. 2022, Biotechnology Notes, 3, 1 - 7, English

  Scientific journal


• Recent advances in lignocellulosic biomass white biotechnology for bioplastics

  Hideo Kawaguchi, Kenji Takada, Taghreed Elkasaby, Radityo Pangestu, Masakazu Toyoshima, Prihardi Kahar, Chiaki Ogino, Tatsuo Kaneko, Akihiko Kondo

  Lignocellulosic biomass has great potential as an inedible feedstock for bioplastic synthesis, although its use is still limited compared to current edible feedstocks of glucose and starch. This review focuses on recent advances in the production of biopolymers and biomonomers from lignocellulosic feedstocks with downstream processing and chemical polymer syntheses. In microbial production, four routes composed of existing poly (lactic acid) and polyhydroxyalkanoates (PHAs) and the emerging biomonomers of itaconic acid and aromatic compounds were presented to review present challenges and future perspectives, focusing on the use of lignocellulosic feedstocks. Recently, advances in purification technologies decreased the number of processes and their environmental burden. Additionally, the unique structures and high-performance of emerging lignocellulose-based bioplastics have expanded the possibilities for the use of bioplastics. The sequence of processes provides insight into the emerging technologies that are needed for the practical use of bioplastics made from lignocellulosic biomass.

  Elsevier BV, Jan. 2022, Bioresource Technology, 344, 126165 - 126165, English, International magazine

  Scientific journal


• Pretreatment of extruded Napier grass by hydrothermal process with dilute sulfuric acid and fermentation using a cellulose-hydrolyzing and xylose-assimilating yeast for ethanol production

  Ku Syahidah Ku Ismail, Yuki Matano, Yuri Sakihama, Kentaro Inokuma, Yumiko Nambu, Tomohisa Hasunuma, Akihiko Kondo

  One of the potential bioresources for bioethanol production is Napier grass, considering its high cellulose and hemicellulose content. However, the cost of pretreatment hinders the bioethanol produced from being economical. This study examines the effect of hydrothermal process with dilute acid on extruded Napier grass, followed by enzymatic saccharification prior to simultaneous saccharification and co-fermentation (SScF). Extrusion facilitated lignin removal by 30.2 % prior to dilute acid steam explosion. Optimum pretreatment condition was obtained by using 3% sulfuric acid, and 30-min retention time of steam explosion at 190 °C. Ethanol yield of 0.26 g ethanol/g biomass (60.5% fermentation efficiency) was attained by short-term liquefaction and fermentation using a cellulose-hydrolyzing and xylose-assimilating Saccharomyces cerevisiae NBRC1440/B-EC3-X ΔPHO13, despite the presence of inhibitors. This proposed method not only reduced over-degradation of cellulose and hemicellulose, but also eliminated detoxification process and reduced cellulase loading.

  Elsevier BV, Jan. 2022, Bioresource Technology, 343, 126071 - 126071, English, International magazine

  Scientific journal


• Nitrogen Availability Affects the Metabolic Profile in Cyanobacteria

  Kosuke Inabe, Ayaka Miichi, Mami Matsuda, Takanobu Yoshida, Yuichi Kato, Ryota Hidese, Akihiko Kondo, Tomohisa Hasunuma

  Nitrogen is essential for the biosynthesis of various molecules in cells, such as amino acids and nucleotides, as well as several types of lipids and sugars. Cyanobacteria can assimilate several forms of nitrogen, including nitrate, ammonium, and urea, and the physiological and genetic responses to these nitrogen sources have been studied previously. However, the metabolic changes in cyanobacteria caused by different nitrogen sources have not yet been characterized. This study aimed to elucidate the influence of nitrate and ammonium on the metabolic profiles of the cyanobacterium Synechocystis sp. strain PCC 6803. When supplemented with NaNO3 or NH4Cl as the nitrogen source, Synechocystis sp. PCC 6803 grew faster in NH4Cl medium than in NaNO3 medium. Metabolome analysis indicated that some metabolites in the CBB cycle, glycolysis, and TCA cycle, and amino acids were more abundant when grown in NH4Cl medium than NaNO3 medium. 15N turnover rate analysis revealed that the nitrogen assimilation rate in NH4Cl medium was higher than in NaNO3 medium. These results indicate that the mechanism of nitrogen assimilation in the GS-GOGAT cycle differs between NaNO3 and NH4Cl. We conclude that the amounts and biosynthetic rate of cyanobacterial metabolites varies depending on the type of nitrogen.

  MDPI AG, Dec. 2021, Metabolites, 11(12) (12), 867 - 867, English, International magazine

  Scientific journal


• Enhanced production of γ-amino acid 3-amino-4-hydroxybenzoic acid by recombinant Corynebacterium glutamicum under oxygen limitation

  Akihiko Kondo

  Dec. 2021, Microbial Cell Factories, 20(1) (1), 228, English

  Scientific journal


• Development of mutant microalgae that accumulate lipids under nitrate-replete conditions

  Akihiko Kondo

  Dec. 2021, Algal Research, 60, 102544 - 102544

  Scientific journal


• Resveratrol production from several types of saccharide sources by a recombinant Scheffersomyces stipitis strain

  Akihiko Kondo

  Dec. 2021, Metabolic Engineering Communications, 13, e00188 - e00188, English

  Scientific journal


• Metabolic engineering design to enhance (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis based on flux balance analysis

  Nunthaphan Vikromvarasiri, Tomokazu Shirai, Akihiko Kondo

  Glycerol is a desirable alternative substrate for 2,3-butanediol (2,3-BD) production for sustainable development in biotechnological industries and non-food competitive feedstock. B. subtilis, a “generally recognized as safe” organism that is highly tolerant to fermentation products, is an ideal platform microorganism to engineer the pathways for the production of valuable bio-based chemicals, but it has never been engineered to improve 2,3-BD production from glycerol. In this study, we aimed to enhance 2,3-BD production from glycerol in B. subtilis through in silico analysis. Genome-scale metabolic model (GSM) simulations was used to design and develop the metabolic pathways of B. subtilis. Flux balance analysis (FBA) simulation was used to evaluate the effects of step-by-step gene knockouts to improve 2,3-BD production from glycerol in B. subtilis. B. subtilis was bioengineered to enhance 2,3-BD production from glycerol using FBA in a published GSM model of B. subtilis, iYO844. Four genes, ackA, pta, lctE, and mmgA, were knocked out step by step, and the effects thereof on 2,3-BD production were evaluated. While knockout of ackA and pta had no effect on 2,3-BD production, lctE knockout led to a substantial increase in 2,3-BD production. Moreover, 2,3-BD production was improved by mmgA knockout, which had never been investigated. In addition, comparisons between in silico simulations and fermentation profiles of all B. subtilis strains are presented in this study. The strategy developed in this study, using in silico FBA combined with experimental validation, can be used to optimize metabolic pathways for enhanced 2,3-BD production from glycerol. It is expected to provide a novel platform for the bioengineering of strains to enhance the bioconversion of glycerol into other highly valuable chemical products.

  Springer Science and Business Media LLC, Dec. 2021, Microbial Cell Factories, 20(1) (1), 196 - 196, English, International magazine

  Scientific journal


• Enhanced methane production from cellulose using a two-stage process involving a bioelectrochemical system and a fixed film reactor

  Kengo Sasaki, Daisuke Sasaki, Yota Tsuge, Masahiko Morita, Akihiko Kondo

  It is desirable to improve the anaerobic digestion processes of recalcitrant materials, such as cellulose. Enhancement of methane (CH₄) production from organic molecules was previously accomplished through coupling a bioelectrochemical system (BES); however, scaling-up BES-based production is difficult. Here, we developed a two-stage process consisting of a BES using low-cost and low-reactive carbon sheets as the cathode and anode, and a fixed film reactor (FFR) containing conductive material, i.e., carbon fiber textiles (CFTs) (:BES → FFR). By controlling the cathodic current at 2.7 μA/cm² without abiotic H₂ production, the three-electrode BES system was operated to mimic a microbial electrolysis cell. The thermophilic BES (inlet pH: 6.1) and FFR (inlet pH: 7.5) were operated using hydraulic retention times (HRTs) of 2.5 and 4.2 days, respectively, corresponding to a cellulose load of 3555.6 mg-carbon (C)/(L day). The BES → FFR process achieved a higher CH₄ yield (37.5%) with 52.8 vol% CH₄ in the product gas compared to the non-bioelectrochemical system (NBES) → FFR process, which showed a CH₄ yield of 22.1% with 46.8 vol% CH₄. The CH₄ production rate (67.5 mM/day) obtained with the BER → FFR process was much higher than that obtained using electrochemical methanogenesis (0.27 mM/day). Application of the electrochemical system or CFTs improved the yields of CH₄ with the NBES → FFR or BES → non-fixed film reactor process, respectively. Meta 16S rRNA sequencing revealed that putative cellulolytic bacteria (identified as Clostridium species) were present in the BES and NBES, and followed (BES→ and NBES→) FFR. Notably, H₂-consuming methanogens, Methanobacterium sp. and Methanosarcina sp., showed increased relative abundances in the suspended fraction and attached fraction of (BES→) FFR, respectively, compared to that of (NBES→) FFR, although these methanogens were observed at trace levels in the BES and NBES. These results indicate that bioelectrochemical preprocessing at a low current effectively induces interspecies H₂ transfer in the FFR with conductive material. Sufficient electrochemical preprocessing was observed using a relatively short HRT. This type of two-stage process, BES → FFR, is useful for stabilization and improvement of the biogas (CH₄) production from cellulosic material, and our results imply that the two-stage system developed here may be useful with other recalcitrant materials.

  Springer Science and Business Media LLC, Dec. 2021, Biotechnology for Biofuels, 14(1) (1)

  Scientific journal


• Disruption of alpha-tubulin releases carbon catabolite repression and enhances enzyme production in Trichoderma reesei even in the presence of glucose

  Nozomu Shibata, Hiroshi Kakeshita, Kazuaki Igarashi, Yasushi Takimura, Yosuke Shida, Wataru Ogasawara, Tohru Koda, Tomohisa Hasunuma, Akihiko Kondo

  Trichoderma reesei is a filamentous fungus that is important as an industrial producer of cellulases and hemicellulases due to its high secretion of these enzymes and outstanding performance in industrial fermenters. However, the reduction of enzyme production caused by carbon catabolite repression (CCR) has long been a problem. Disruption of a typical transcriptional regulator, Cre1, does not sufficiently suppress this reduction in the presence of glucose. We found that deletion of an α-tubulin (tubB) in T. reesei enhanced both the amount and rate of secretory protein production. Also, the tubulin-disrupted (ΔtubB) strain had high enzyme production and the same enzyme profile even if the strain was cultured in a glucose-containing medium. From transcriptome analysis, the ΔtubB strain exhibited upregulation of both cellulase and hemicellulase genes including some that were not originally induced by cellulose. Moreover, cellobiose transporter genes and the other sugar transporter genes were highly upregulated, and simultaneous uptake of glucose and cellobiose was also observed in the ΔtubB strain. These results suggested that the ΔtubB strain was released from CCR. Trichoderma reesei α-tubulin is involved in the transcription of cellulase and hemicellulase genes, as well as in CCR. This is the first report of overcoming CCR by disrupting α-tubulin gene in T. reesei. The disruption of α-tubulin is a promising approach for creating next-generation enzyme-producing strains of T. reesei.

  Springer Science and Business Media LLC, Dec. 2021, Biotechnology for Biofuels, 14(1) (1), 39 - 39, English, International magazine

  Scientific journal


• Enhancing carbohydrate repartitioning into lipid and carotenoid by disruption of microalgae starch debranching enzyme

  Yuichi Kato, Tomoki Oyama, Kentaro Inokuma, Christopher J. Vavricka, Mami Matsuda, Ryota Hidese, Katsuya Satoh, Yutaka Oono, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo

  Light/dark cycling is an inherent condition of outdoor microalgae cultivation, but is often unfavorable for lipid accumulation. This study aims to identify promising targets for metabolic engineering of improved lipid accumulation under outdoor conditions. Consequently, the lipid-rich mutant Chlamydomonas sp. KOR1 was developed through light/dark-conditioned screening. During dark periods with depressed CO₂ fixation, KOR1 shows rapid carbohydrate degradation together with increased lipid and carotenoid contents. KOR1 was subsequently characterized with extensive mutation of the ISA1 gene encoding a starch debranching enzyme (DBE). Dynamic time-course profiling and metabolomics reveal dramatic changes in KOR1 metabolism throughout light/dark cycles. During light periods, increased flux from CO₂ through glycolytic intermediates is directly observed to accompany enhanced formation of small starch-like particles, which are then efficiently repartitioned in the next dark cycle. This study demonstrates that disruption of DBE can improve biofuel production under light/dark conditions, through accelerated carbohydrate repartitioning into lipid and carotenoid.

  Springer Science and Business Media LLC, Dec. 2021, Communications Biology, 4(1) (1), 450 - 450, English, International magazine

  Scientific journal


• Growth stimulation of Bifidobacterium from human colon using daikenchuto in an in vitro model of human intestinal microbiota

  Akihiko Kondo

  Dec. 2021, Scientific Reports, 11(1) (1), English

  Scientific journal


• Increased carvone production in Escherichia coli by balancing limonene conversion enzyme expression via targeted quantification concatamer proteome analysis.

  Erika Yoshida, Motoki Kojima, Munenori Suzuki, Fumio Matsuda, Kazutaka Shimbo, Akiko Onuki, Yousuke Nishio, Yoshihiro Usuda, Akihiko Kondo, Jun Ishii

  (-)-Carvone is a monoterpenoid with a spearmint flavor. A sustainable biotechnological production process for (-)-carvone is desirable. Although all enzymes in (-)-carvone biosynthesis have been functionally expressed in Escherichia coli independently, the yield was low in previous studies. When cytochrome P450 limonene-6-hydroxylase (P450)/cytochrome P450 reductase (CPR) and carveol dehydrogenase (CDH) were expressed in a single strain, by-product formation (dihydrocarveol and dihydrocarvone) was detected. We hypothesized that P450 and CDH expression levels differ in E. coli. Thus, two strains independently expressing P450/CPR and CDH were mixed with different ratios, confirming increased carvone production and decreased by-product formation when CDH input was reduced. The optimum ratio of enzyme expression to maximize (-)-carvone production was determined using the proteome analysis quantification concatamer (QconCAT) method. Thereafter, a single strain expressing both P450/CPR and CDH was constructed to imitate the optimum expression ratio. The upgraded strain showed a 15-fold improvement compared to the initial strain, showing a 44 ± 6.3 mg/L (-)-carvone production from 100 mg/L (-)-limonene. Our study showed the usefulness of the QconCAT proteome analysis method for strain development in the industrial biotechnology field.

  Nov. 2021, Scientific reports, 11(1) (1), 22126 - 22126, English, International magazine

  Scientific journal


• Bacteroides spp. promotes branched-chain amino acid catabolism in brown fat and inhibits obesity

  Naofumi Yoshida, Tomoya Yamashita, Tatsunori Osone, Tetsuya Hosooka, Masakazu Shinohara, Seiichi Kitahama, Kengo Sasaki, Daisuke Sasaki, Takeshi Yoneshiro, Tomohiro Suzuki, Takuo Emoto, Yoshihiro Saito, Genki Ozawa, Yushi Hirota, Yasuyuki Kitaura, Yoshiharu Shimomura, Yuko Okamatsu-Ogura, Masayuki Saito, Akihiko Kondo, Shingo Kajimura, Takeshi Inagaki, Wataru Ogawa, Takuji Yamada, Ken-ichi Hirata

  The gut microbiome has emerged as a key regulator of obesity; however, its role in brown adipose tissue (BAT) metabolism and association with obesity remain to be elucidated. We found that the levels of circulating branched-chain amino acids (BCAA) and their cognate α-ketoacids (BCKA) were significantly correlated with the body weight in humans and mice and that BCAA catabolic defects in BAT were associated with obesity in diet-induced obesity (DIO) mice. Pharmacological systemic enhancement of BCAA catabolic activity reduced plasma BCAA and BCKA levels and protected against obesity; these effects were reduced in BATectomized mice. DIO mice gavaged with Bacteroides dorei and Bacteroides vulgatus exhibited improved BAT BCAA catabolism and attenuated body weight gain, which were not observed in BATectomized DIO mice. Our data have highlighted a possible link between the gut microbiota and BAT BCAA catabolism and suggest that Bacteroides probiotics could be used for treating obesity.

  Elsevier BV, Nov. 2021, iScience, 24(11) (11), 103342 - 103342, English, International magazine

  Scientific journal


• Salinity-induced microalgal-based mariculture wastewater treatment combined with biodiesel production

  Akihiko Kondo

  Nov. 2021, Bioresource Technology, 340, 125638 - 125638, English

  Scientific journal


• 13C-metabolic flux analysis in glycerol-assimilating strains of Saccharomyces cerevisiae

  Taiji Yuzawa, Tomokazu Shirai, Ryoko Orishimo, Kazuki Kawai, Akihiko Kondo, Takashi Hirasawa

  Glycerol is an attractive raw material for the production of useful chemicals using microbial cells. We previously identified metabolic engineering targets for the improvement of glycerol assimilation ability in Saccharomyces cerevisiae based on adaptive laboratory evolution (ALE) and transcriptome analysis of the evolved cells. We also successfully improved glycerol assimilation ability by the disruption of the RIM15 gene encoding a Greatwall protein kinase together with overexpression of the STL1 gene encoding the glycerol/H+ symporter. To understand glycerol assimilation metabolism in the evolved glycerol-assimilating strains and STL1-overexpressing RIM15 disruptant, we performed metabolic flux analysis using 13C-labeled glycerol. Significant differences in metabolic flux distributions between the strains obtained from the culture after 35 and 85 generations in ALE were not found, indicating that metabolic flux changes might occur in the early phase of ALE (i.e., before 35 generations at least). Similarly, metabolic flux distribution was not significantly changed by RIM15 gene disruption. However, fluxes for the lower part of glycolysis and the TCA cycle were larger and, as a result, flux for the pentose phosphate pathway was smaller in the STL1-overexpressing RIM15 disruptant than in the strain obtained from the culture after 85 generations in ALE. It could be effective to increase flux for the pentose phosphate pathway to improve the glycerol assimilation ability in S. cerevisiae.

  Microbiology Research Foundation, Oct. 2021, The Journal of General and Applied Microbiology, 67(4) (4), 142 - 149, English, Domestic magazine

  Scientific journal


• Cell surface engineering of Saccharomyces cerevisiae for simultaneous valorization of corn cob and cheese whey via ethanol production

  Akihiko Kondo

  Sep. 2021, Energy Conversion and Management, 243, 114359 - 114359, English

  Scientific journal


• Reprogramming Escherichia coli pyruvate-forming reaction towards chorismate derivatives production

  Shuhei Noda, Yutaro Mori, Ryosuke Fujiwara, Tomokazu Shirai, Tsutomu Tanaka, Akihiko Kondo

  Microbial metabolic pathway engineering is a potent strategy used worldwide to produce aromatic compounds. We drastically rewired the primary metabolic pathway of Escherichia coli to produce aromatics and their derivatives. The metabolic pathway of E. coli was compartmentalized into the production and energy modules. We focused on the pyruvate-forming reaction in the biosynthesis pathway of some compounds as the reaction connecting those modules. E. coli strains were engineered to show no growth unless pyruvate was synthesized along with the compounds of interest production. Production of salicylate and maleate was demonstrated to confirm our strategy's versatility. In maleate production, the production, yield against the theoretical yield, and production rate reached 12.0 g L-1, 67%, and up to fourfold compared to that in previous reports, respectively; these are the highest values of maleate production in microbes to our knowledge. The results reveal that our strategy strongly promotes the production of aromatics and their derivatives.

  Elsevier BV, Sep. 2021, Metabolic Engineering, 67, 1 - 10, English, International magazine

  Scientific journal


• Evaluation of Aqueous Biphasic Electrophoresis System Based on Halide-Free Ionic Liquids for Direct Recovery of Keratinase

  Akihiko Kondo

  Aug. 2021, Marine Drugs, 19(8) (8), 463 - 463, English

  Scientific journal


• Incorporation of electric fields to ionic liquids-based aqueous biphasic system for enhanced recovery of extracellular Kytococcus sedentarius TWHKC01 keratinase

  Akihiko Kondo

  Aug. 2021, Journal of the Taiwan Institute of Chemical Engineers, 125, 35 - 40, English

  Scientific journal


• Transversion Expansion of Base Editing.

  Keiji Nishida, Akihiko Kondo

  Aug. 2021, The CRISPR journal, 4(4) (4), 462 - 463, English, International magazine

  Scientific journal


• Improving the functionality of surface-engineered yeast cells by altering the cell wall morphology of the host strain

  Akihiko Kondo

  Aug. 2021, Applied Microbiology and Biotechnology, 105(14-15) (14-15), 5895 - 5904, English

  Scientific journal


• Accelerated glucose metabolism in hyphae-dispersed Aspergillus oryzae is suitable for biological production

  Taku Sakuragawa, Satoshi Wakai, Silai Zhang, Hideo Kawaguchi, Chiaki Ogino, Akihiko Kondo

  Recently, a hyphae-dispersed type of filamentous fungus Aspergillus oryzae was constructed via genetic engineering, and industrial applications are expected due to the ease of handling and to the level of protein production properties. In this study, we constructed cellulase-expressing strains using wild-type and hyphae-dispersed strains to investigate the correlation between protein productivity and metabolism. Compared with the original strain, the hyphae-dispersed cellulase-expressing strain showed elevated cellulase activity, rapid glucose consumption, increased mycelial dry weight, an increased expression of cellulase genes, and activated respiration activity. Comparative metabolomic analysis showed fewer metabolites in the glycolysis and TCA cycles in the dispersed strains than in the original strains. These results indicate that the flux of carbohydrate metabolism in the hyphae-dispersed strains is smoother than that in the original strains. Such efficient metabolic flux would contribute to efficient energy conversion and to sufficient energy supply to anabolisms, such as mycelial growth and protein production. Our findings suggest that the hyphae-dispersed strains could be a useful host not only for protein production but also for the biological production of various chemicals such as organic acids.

  Elsevier BV, Aug. 2021, Journal of Bioscience and Bioengineering, 132(2) (2), 140 - 147, English, Domestic magazine

  Scientific journal


• Lutein production with Chlorella sorokiniana MB-1-M12 using novel two-stage cultivation strategies – metabolic analysis and process improvement

  Jih-Heng Chen, Yuichi Kato, Mami Matsuda, Chun-Yen Chen, Dillirani Nagarajan, Tomohisa Hasunuma, Akihiko Kondo, Jo-Shu Chang

  Microalgae-derived carotenoids have increasingly been considered as feasible green alternatives for synthetic antioxidants. In this study, the lutein high-yielding strain (Chlorella sorokiniana MB-1; henceforth MB-1) and its mutant derivative (C. sorokiniana MB-1-M12; henceforth M12) were evaluated for their growth, biomass production, and lutein accumulation in three different cultivation modes - photoautotrophy, mixotrophy, and heterotrophy. M12 could grow effectively under heterotrophic conditions, but the lutein content was lower, indicating the necessity of photo-induction for lutein accumulation. Metabolic analysis of MB-1 and M12 in autotrophic growth in the presence of carbon dioxide indicated that carbon assimilation and channeling of the fixed metabolites towards carotenoid accumulation was elevated in M12 compared to MB-1. Novel two-stage alternative cultivation strategies (Autotrophic/Heterotrophic and Mixotrophic/Heterotrophic cultures) were applied for enhancing lutein production in M12. Maximum lutein quantity (6.17 mg/g) and production (33.64 mg/L) were obtained with the TSHM strategy that is considered the best two-stage operation.

  Elsevier BV, Aug. 2021, Bioresource Technology, 334, 125200 - 125200, English, International magazine

  Scientific journal


• Advances in metabolic engineering of Corynebacterium glutamicum to produce high-value active ingredients for food, feed, human health, and well-being

  Sabrina Wolf, Judith Becker, Yota Tsuge, Hideo Kawaguchi, Akihiko Kondo, Jan Marienhagen, Michael Bott, Volker F. Wendisch, Christoph Wittmann

  The soil microbe Corynebacterium glutamicum is a leading workhorse in industrial biotechnology and has become famous for its power to synthetise amino acids and a range of bulk chemicals at high titre and yield. The product portfolio of the microbe is continuously expanding. Moreover, metabolically engineered strains of C. glutamicum produce more than 30 high value active ingredients, including signature molecules of raspberry, savoury, and orange flavours, sun blockers, anti-ageing sugars, and polymers for regenerative medicine. Herein, we highlight recent advances in engineering of the microbe into novel cell factories that overproduce these precious molecules from pioneering proofs-of-concept up to industrial productivity.

  Portland Press Ltd., Jun. 2021, Essays in Biochemistry, 65(2) (2), 197 - 212, English, International magazine

  Scientific journal


• Synthetic production of prenylated naringenins in yeast using promiscuous microbial prenyltransferases

  Shota Isogai, Nobuyuki Okahashi, Ririka Asama, Tomomi Nakamura, Tomohisa Hasunuma, Fumio Matsuda, Jun Ishii, Akihiko Kondo

  Reconstitution of prenylflavonoids using the flavonoid biosynthetic pathway and prenyltransferases (PTs) in microbes can be a promising attractive alternative to plant-based production or chemical synthesis. Here, we demonstrate that promiscuous microbial PTs can be a substitute for regiospecific but mostly unidentified botanical PTs. To test the prenylations of naringenin, we constructed a yeast strain capable of producing naringenin from l-phenylalanine by genomic integration of six exogenous genes encoding components of the naringenin biosynthetic pathway. Using this platform strain, various microbial PTs were tested for prenylnaringenin production. In vitro screening demonstrated that the fungal AnaPT (a member of the tryptophan dimethylallyltransferase family) specifically catalyzed C-3' prenylation of naringenin, whereas SfN8DT-1, a botanical PT, specifically catalyzed C-8 prenylation. In vivo, the naringenin-producing strain expressing the microbial AnaPT exhibited heterologous microbial production of 3'-prenylnaringenin (3'-PN), in contrast to the previously reported in vivo production of 8-prenylnaringenin (8-PN) using the botanical SfN8DT-1. These findings provide strategies towards expanding the production of a variety of prenylated compounds, including well-known prenylnaringenins and novel prenylflavonoids. These results also suggest the opportunity for substituting botanical PTs, both known and unidentified, that display relatively strict regiospecificity of the prenyl group transfer.

  Elsevier BV, Jun. 2021, Metabolic Engineering Communications, 12, e00169 - e00169, English, International magazine

  Scientific journal


• Four-carbon dicarboxylic acid production through the reductive branch of the open cyanobacterial tricarboxylic acid cycle in Synechocystis sp. PCC 6803

  Hiroko Iijima, Atsuko Watanabe, Haruna Sukigara, Kaori Iwazumi, Tomokazu Shirai, Akihiko Kondo, Takashi Osanai

  Succinate, fumarate, and malate are valuable four-carbon (C4) dicarboxylic acids used for producing plastics and food additives. C4 dicarboxylic acid is biologically produced by heterotrophic organisms. However, current biological production requires organic carbon sources that compete with food uses. Herein, we report C4 dicarboxylic acid production from CO2 using metabolically engineered Synechocystis sp. PCC 6803. Overexpression of citH, encoding malate dehydrogenase (MDH), resulted in the enhanced production of succinate, fumarate, and malate. citH overexpression increased the reductive branch of the open cyanobacterial tricarboxylic acid (TCA) cycle flux. Furthermore, product stripping by medium exchanges increased the C4 dicarboxylic acid levels; product inhibition and acidification of the media were the limiting factors for succinate production. Our results demonstrate that MDH is a key regulator that activates the reductive branch of the open cyanobacterial TCA cycle. The study findings suggest that cyanobacteria can act as a biocatalyst for converting CO2 to carboxylic acids.

  Elsevier BV, May 2021, Metabolic Engineering, 65, 88 - 98, English, International magazine

  Scientific journal


• Utilizing palm oil mill effluent (POME) for the immobilization of Aspergillus oryzae whole-cell lipase strains for biodiesel synthesis

  Akihiko Kondo

  May 2021, Biofuels, Bioproducts and Biorefining, 15(3) (3), 804 - 814, English

  Scientific journal


• Metabolic design for selective production of nicotinamide mononucleotide from glucose and nicotinamide

  Akihiko Kondo

  May 2021, Metabolic Engineering, 65, 167 - 177

  Scientific journal


• Cytosine base editing systems with minimized off-target effect and molecular size

  Ang Li, Hitoshi Mitsunobu, Shin Yoshioka, Suzuki Takahisa, Akihiko Kondo, Keiji Nishida

  Abstract Structure-based rational engineering of the cytosine base editing system Target-AID was performed to minimize its off-target effect and molecular size. By intensive and careful truncation, DNA-binding domain of its deaminase PmCDA1 was eliminated and additional mutations were introduced to restore enzyme function. The resulting tCDA1EQ was effective in N-terminal fusion (AID-2S) or inlaid architecture (AID-3S) with Cas9, showing minimized gRNA-independent off-targets, as assessed in yeast and human cells. Combining with the smaller Cas9 ortholog system, the smallest cytosine base editing system was created that is within the size limit of AAV vector.

  Research Square Platform LLC, Apr. 2021


• Development of a Method for Fucoxanthin Production Using the Haptophyte Marine Microalga Pavlova sp. OPMS 30543.

  Akihiko Kanamoto, Yuichi Kato, Erina Yoshida, Tomohisa Hasunuma, Akihiko Kondo

  The natural pigment fucoxanthin has attracted global attention because of its superior antioxidant properties. The haptophyte marine microalgae Pavlova spp. are assumed to be promising industrial fucoxanthin producers as their lack of a cell wall could facilitate the commercialization of cultured cells as a whole food. This study screened promising Pavlova strains with high fucoxanthin content to develop an outdoor cultivation method for fucoxanthin production. Initial laboratory investigations of P. pinguis NBRC 102807, P. lutheri NBRC 102808, and Pavlova sp. OPMS 30543 identified OPMS 30543 as having the highest fucoxanthin content. The culture conditions were optimized for OPMS 30543. Compared with f/2 and Walne's media, the use of Daigo's IMK medium led to the highest biomass production and highest fucoxanthin accumulation. The presence of seawater elements in Daigo's IMK medium was necessary for the growth of OPMS 30543. OPMS 30543 was then cultured outdoors using acrylic pipe photobioreactors, a plastic bag, an open tank, and a raceway pond. Acrylic pipe photobioreactors with small diameters enabled the highest biomass production. Using an acrylic pipe photobioreactor with 60-mm diameter, a fucoxanthin productivity of 4.88 mg/L/day was achieved in outdoor cultivation. Thus, this study demonstrated the usefulness of Pavlova sp. OPMS 30543 for fucoxanthin production in outdoor cultivation.

  Apr. 2021, Marine biotechnology (New York, N.Y.), 23(2) (2), 331 - 341, English, International magazine

  Scientific journal


• Efficient base editing in tomato using a highly expressed transient system

  Shaoze Yuan, Shunsuke Kawasaki, Islam M. Y. Abdellatif, Keiji Nishida, Akihiko Kondo, Tohru Ariizumi, Hiroshi Ezura, Kenji Miura

  KEY MESSAGE: Base editing in tomatoes was achieved by transient expression. The Solanaceae plants, particularly the tomato (Solanum lycopersicum), is of huge economic value worldwide. The tomato is a unique model plant for studying the functions of genes related to fruit ripening. Deeper understanding of tomatoes is of great importance for both plant research and the economy. Genome editing technology, such as CRISPR/Cas9, has been used for functional genetic research. However, some challenges, such as low transformation efficiency, remain with this technology. Moreover, the foreign Cas9 and gRNA expression cassettes must be removed to obtain null-segregants In this study, we used a high-level transient expression system to improve the base editing technology. A high-level transient expression system has been established previously using geminiviral replication and a double terminator. The pBYR2HS vector was used for this transient expression system. nCas9-CDA and sgRNA-SlHWS were introduced into this vector, and the protein and RNA were then transiently expressed in tomato tissues by agroinfiltration. The homozygous mutant produced by base editing was obtained in the next generation with an efficiency of about 18%. nCas9-free next-generation plants were 71%. All the homozygous base-edited plants in next generation are nCas9-free. These findings show that the high-level transient expression system is useful for base editing in tomatoes.

  Springer Science and Business Media LLC, Apr. 2021, Plant Cell Reports, 40(4) (4), 667 - 676, English, International magazine

  Scientific journal


• Direct 1,3-butadiene biosynthesis in Escherichia coli via a tailored ferulic acid decarboxylase mutant

  Yutaro Mori, Shuhei Noda, Tomokazu Shirai, Akihiko Kondo

  Apr. 2021, NATURE COMMUNICATIONS, 12(1) (1), 2195, English

  Scientific journal


• W27 IgA suppresses growth of Escherichia in an in vitro model of the human intestinal microbiota

  Akihiko Kondo

  Mar. 2021, Scientific Reports, English

  Scientific journal


• Future trends in synthetic biology in Asia

  Ning Mao, Nikhil Aggarwal, Chueh Loo Poh, Byung Kwan Cho, Akihiko Kondo, Chenli Liu, Wen Shan Yew, Matthew Wook Chang

  Wiley, Mar. 2021, Advanced Genetics, 2(1) (1), English

  Scientific journal


• Robust and flexible platform for directed evolution of yeast genetic switches

  Masahiro Tominaga, Kenta Nozaki, Daisuke Umeno, Jun Ishii, Akihiko Kondo

  Mar. 2021, Nature Communications, 12(1) (1), English

  Scientific journal


• Titanium oxide nano-radiosensitizers for hydrogen peroxide delivery into cancer cells

  Kenta Morita, Yuya Nishimura, Satoko Nakamura, Yuki Arai, Chiya Numako, Kazuyoshi Sato, Masao Nakayama, Hiroaki Akasaka, Ryohei Sasaki, Chiaki Ogino, Akihiko Kondo

  Feb. 2021, Colloids and Surfaces B: Biointerfaces, 198, 111451 - 111451, English

  Scientific journal


• Comparative analyses of site‐directed mutagenesis of human melatonin MTNR1A and MTNR1B receptors using a yeast fluorescent biosensor

  Yasuyuki Nakamura, Ririka Asama, Takuya Tabata, Kenta Morita, Tatsuo Maruyama, Akihiko Kondo, Jun Ishii

  Feb. 2021, Biotechnology and Bioengineering, 118(2) (2), 863 - 876, English

  Scientific journal


• Semiological differences of focal onset bilateral motor (convulsive) seizure between mesial temporal lobe epilepsy and neocortical epilepsy

  Akihiko Kondo

  2021, Epilepsy Research

  Scientific journal


• The Changes in Concentration of Cerebral Oxygenated Hemoglobin During Single Event-Related Japanese Shiritori Task in Patients With Major Depression Disorder: Comparison With Healthy Subjects

  Akihiko Kondo

  2021, Frontiers in Psychiatry

  Scientific journal


• Neuropsychological outcomes after frontal lobectomy to treat intractable epilepsy

  Akihiko Kondo

  2021, Epilepsy & Behavior

  Scientific journal


• Exophiala dermatitidis

  Akihiko Kondo

  2021, World Journal of Clinical Cases

  Scientific journal


• Impairment of emotional expression detection after unilateral medial temporal structure resection

  Akihiko Kondo

  2021, Scientific Reports

  Scientific journal


• Diffuse large B cell lymphoma originating from the maxillary sinus with skin metastases: A case report and review of literature

  Akihiko Kondo

  2021, World Journal of Clinical Cases

  Scientific journal


• Angiotensin receptor blocker neprilysin inhibitors

  Akihiko Kondo

  2021, World Journal of Cardiology

  Scientific journal


• Constitutive cell surface expression of ZZ domain for the easy preparation of yeast-based immunosorbents

  Kohei Katsurada, Masahiro Tominaga, Misato Kaishima, Hiroko Kato, Toshihide Matsuno, Chiaki Ogino, Akihiko Kondo, Jun Ishii, Katsumi Takayama

  We describe a novel expression cassette that enables efficient and constitutive expression of the ZZ domain derived from Staphylococcus aureus protein A on the yeast cell surface to easily prepare yeast-based immunosorbents. Using this expression cassette containing the PGK1 promoter, a secretion signal derived from α-factor, and a Flo1-derived anchor protein, we successfully created a yeast-based immunosorbent for human serum albumin.

  Microbiology Research Foundation, 2021, The Journal of General and Applied Microbiology, 67(6) (6), 265 - 268, English, Domestic magazine

  Scientific journal


• CRISPR-derived genome editing technologies for metabolic engineering

  Keiji Nishida, Akihiko Kondo

  In metabolic engineering, genome editing tools make it much easier to discover and evaluate relevant genes and pathways and construct strains. Clustered regularly interspaced palindromic repeats (CRISPR)-associated (Cas) systems now have become the first choice for genome engineering in many organisms includingindustrially relevant ones. Targeted DNA cleavage by CRISPR-Cas provides variousgenome engineering modes such as indels, replacements, large deletions, knock-in and chromosomal rearrangements, while host-dependent differences in repair pathways need to be considered. The versatility of the CRISPR system has given rise to derivative technologies that complement nuclease-based editing, which causes cytotoxicity especially in microorganisms. Deaminase-mediated base editing installs targeted point mutations with much less toxicity. CRISPRi and CRISPRa can temporarily control gene expression without changing the genomic sequence. Multiplex, combinatorial and large scale editing are made possible by streamlined design and construction of gRNA libraries to further accelerates comprehensive discovery, evaluation and building of metabolic pathways. This review summarizes the technical basis and recent advances in CRISPR-related genome editing tools applied for metabolic engineering purposes, with representative examples of industrially relevant eukaryotic and prokaryotic organisms.

  Elsevier BV, Jan. 2021, Metabolic Engineering, 63, 141 - 147, English, International magazine

  Scientific journal


• Ultrahigh Thermoresistant Lightweight Bioplastics Developed from Fermentation Products of Cellulosic Feedstock

  Akihiko Kondo

  Jan. 2021, Advanced Sustainable Systems, 5(1) (1), 2000193 - 2000193, English

  Scientific journal


• The alga Euglena gracilis stimulates Faecalibacterium in the gut and contributes to increased defecation

  Akihiko Kondo

  Jan. 2021, Scientific Reports, 11(1) (1), English

  Scientific journal


• Optimization of 1,2,4‐butanetriol production from xylose in Saccharomyces cerevisiae by metabolic engineering of NADH/NADPH balance

  Takahiro Yukawa, Takahiro Bamba, Gregory Guirimand, Mami Matsuda, Tomohisa Hasunuma, Akihiko Kondo

  Wiley, Jan. 2021, Biotechnology and Bioengineering, 118(1) (1), 175 - 185, English

  Scientific journal


• An ion-pair free LC-MS/MS method for quantitative metabolite profiling of microbial bioproduction systems

  Akihiko Kondo

  Jan. 2021, Talanta, 222, 121625 - 121625, English

  Scientific journal


• Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression

  Akihiko Kondo

  Dec. 2020, Nucleic Acids Research, 48(22) (22), 13000 - 13012, English

  Scientific journal


• Multiple gene substitution by Target-AID base-editing technology in tomato

  Johan Hunziker, Keiji Nishida, Akihiko Kondo, Sanae Kishimoto, Tohru Ariizumi, Hiroshi Ezura

  The use of Target activation-induced cytidine deaminase (Target-AID) base-editing technology with the CRISPR-Cas 9 system fused with activation-induced cytidine deaminase (AID) resulted in the substitution of a cytidine with a thymine. In previous experiments focusing on a single target gene, this system has been reported to work in several plant species, including tomato (Solanum lycopersicum L.). In this research, we used Target-AID technology to target multiple genes related to carotenoid accumulation in tomato. We selected 3 genes, SlDDB1, SlDET1 and SlCYC-B, for their roles in carotenoid accumulation. Among 12 edited T₀ lines, we obtained 10 independent T₀ lines carrying nucleotide substitutions in the three targeted genes, with several allelic versions for each targeted gene. The two edited lines showed significant differences in carotenoid accumulation. These results demonstrate that Target-AID technology is a highly efficient tool for targeting multiple genes with several allelic versions.

  Springer Science and Business Media LLC, Dec. 2020, Scientific Reports, 10(1) (1), 20471 - 20471, English, International magazine

  Scientific journal


• Immobilized lipases for biodiesel production: Current and future greening opportunities

  Akihiko Kondo

  Dec. 2020, Renewable and Sustainable Energy Reviews, 134, 110355 - 110355, English

  Scientific journal


• A possible beneficial effect of Bacteroides on faecal lipopolysaccharide activity and cardiovascular diseases

  Naofumi Yoshida, Tomoya Yamashita, Shigenobu Kishino, Hikaru Watanabe, Kengo Sasaki, Daisuke Sasaki, Tokiko Tabata, Yuta Sugiyama, Nahoko Kitamura, Yoshihiro Saito, Takuo Emoto, Tomohiro Hayashi, Tomoya Takahashi, Masakazu Shinohara, Ro Osawa, Akihiko Kondo, Takuji Yamada, Jun Ogawa, Ken-ichi Hirata

  Springer Science and Business Media LLC, Dec. 2020, Scientific Reports, 10(1) (1), 13009, English

  [Refereed]

  Scientific journal


• In vitro human colonic microbiota utilises D-β-hydroxybutyrate to increase butyrogenesis

  Kengo Sasaki, Daisuke Sasaki, Asuka Hannya, Jun Tsubota, Akihiko Kondo

  Springer Science and Business Media LLC, Dec. 2020, Scientific Reports, 10(1) (1), 8516, English

  [Refereed]

  Scientific journal


• In Vivo Evaluation of the ZHER2-BNC/LP Carrier Encapsulating an Anticancer Drug and a Radiosensitizer

  Yuya Nishimura, Ryosuke Ezawa, Kenta Morita, Masao Nakayama, Jun Ishii, Ryohei Sasaki, Chiaki Ogino, Akihiko Kondo

  Nov. 2020, ACS Applied Bio Materials, 3(11) (11), 7743 - 7751, English

  Scientific journal


• Valorization of palm biomass waste into carbon matrices for the immobilization of recombinant Fusarium heterosporum lipase towards palm biodiesel synthesis

  Emmanuel Quayson, Jerome Amoah, Nova Rachmadona, Kenta Morita, Lawrence Darkwah, Shinji Hama, Ayumi Yoshida, Akihiko Kondo, Chiaki Ogino

  Nov. 2020, Biomass and Bioenergy, 142, 105768 - 105768, English

  [Refereed]

  Scientific journal


• Prebiotic effects of yeast mannan, which selectively promotes Bacteroides thetaiotaomicron and Bacteroides ovatus in a human colonic microbiota model

  Akihiko Kondo

  Oct. 2020, Scientific Reports, 10(1) (1), 17351, English

  Scientific journal


• 食物中の毒性物質検出のためのパトロール酵母の開発(Development of patrol yeasts for the detection of toxic substances in foods)

  蘇 九龍, 中村 泰之, 北口 哲也, 石井 純, 近藤 昭彦, 小林 典裕, 三宅 司郎, 上田 宏

  (公社)日本生化学会, Sep. 2020, 日本生化学会大会プログラム・講演要旨集, 93回, [3Z09 - 509)], English


• Complete and draft genome sequences of amino acid-producing Corynebacterium glutamicum strains ATCC 21799 and ATCC 31831 and their genomic islands

  Hideo Kawaguchi, Takashi Sazuka, Akihiko Kondo

  We determined the complete and draft genome sequences of two strains of Corynebacterium glutamicum and revealed their genomic islands (GEIs). The two strains, ATCC 21799 and ATCC 31831, were found to have 3,079 and 3,109 coding sequences, respectively, with 13 GEIs each not present in the reference strain, ATCC 13032.

  American Society for Microbiology, Aug. 2020, Microbiology Resource Announcements, 9(32) (32), e00430-20, English

  [Refereed]

  Scientific journal


• Optimal Ratio of Carbon Flux between Glycolysis and the Pentose Phosphate Pathway for Amino Acid Accumulation in Corynebacterium glutamicum.

  Katsuki Murai, Daisuke Sasaki, Shunsuke Kobayashi, Akira Yamaguchi, Hiroto Uchikura, Tomokazu Shirai, Kengo Sasaki, Akihiko Kondo, Yota Tsuge

  Glucose is metabolized through central metabolic pathways such as glycolysis and the pentose phosphate pathway (PPP) to synthesize downstream metabolites including amino acids. However, how the split ratio of carbon flux between glycolysis and PPP specifically affects the formation of downstream metabolites remains largely unclear. Here, we conducted a comprehensive metabolomic analysis to investigate the effect of the split ratio between glycolysis and the PPP on the intracellular concentration of amino acids and their derivatives in Corynebacterium glutamicum. The split ratio was varied by exchanging the promoter of a gene encoding glucose 6-phosphate isomerase (PGI). The ratio was correlated with the pgi transcription level and the enzyme activity. Concentrations of threonine and lysine-derivative 1,5-diaminopentane increased with an increase of the split ratio into the PPP. In contrast, concentrations of alanine, leucine, and valine were increased with an increase of the split ratio into glycolysis. These results could provide a new engineering target for improving the production of the amino acids and the derivatives.

  American Chemical Society (ACS), Jul. 2020, ACS synthetic biology, 9(7) (7), 1615 - 1622, English, International magazine

  [Refereed]

  Scientific journal


• Pyruvate metabolism redirection for biological production of commodity chemicals in aerobic fungus Aspergillus oryzae.

  Silai Zhang, Satoshi Wakai, Naoya Sasakura, Hiroko Tsutsumi, Yoji Hata, Chiaki Ogino, Akihiko Kondo

  Pyruvate is a central metabolite for the biological production of various chemicals. In eukaryotes, pyruvate produced by glycolysis is used in conversion to ethanol and lactate and in anabolic metabolism in the cytosol, or is transported into the mitochondria for use as a substrate in the tricarboxylic acid (TCA) cycle. In this study, we focused on controlling pyruvate metabolism in aerobic microorganisms for the biological production of various chemicals. We successfully improved productivity by redirecting pyruvate metabolism in the aerobic filamentous fungus Aspergillus oryzae via the deletion of two genes that encode pyruvate decarboxylase and mitochondrial pyruvate carriers. Production of ethanol as a major byproduct was completely inhibited, and the limited translocation of pyruvate into the mitochondria shifted the metabolism from respiration for energy conversion to the effective production of lactate or 2,3-butandiole, even under aerobic conditions. Metabolomic and transcriptomic analyses showed an emphasis on glycolysis and a repressed TCA cycle. Although the dry mycelial weights of the deletion mutants were reduced compared with those of wild type, the titer and yields of the target products were drastically increased. In particular, the redirection of pyruvate metabolism shifted from anabolism for biomass production to catabolism for the production of target chemicals. Conclusively, our results indicate that the redirection of pyruvate metabolism is a useful strategy in the metabolic engineering of aerobic microorganisms.

  Elsevier BV, Jul. 2020, Metabolic engineering, 61, 225 - 237, English, International magazine

  [Refereed]

  Scientific journal


• Consolidated bioprocessing of corn cob-derived hemicellulose: engineered industrial Saccharomyces cerevisiae as efficient whole cell biocatalysts

  Joana T. Cunha, Aloia Romaní, Kentaro Inokuma, Björn Johansson, Tomohisa Hasunuma, Akihiko Kondo, Lucília Domingues

  Consolidated bioprocessing, which combines saccharolytic and fermentative abilities in a single microorganism, is receiving increased attention to decrease environmental and economic costs in lignocellulosic biorefineries. Nevertheless, the economic viability of lignocellulosic ethanol is also dependent of an efficient utilization of the hemicellulosic fraction, which is mainly composed of xylose and may comprise up to 40 % of the total biomass. This major bottleneck is mainly due to the necessity of chemical/enzymatic treatments to hydrolyze hemicellulose into fermentable sugars and to the fact that xylose is not readily consumed by Saccharomyces cerevisiae – the most used organism for large-scale ethanol production. In this work, industrial S. cerevisiae strains, presenting robust traits such as thermotolerance and improved resistance to inhibitors, were evaluated as hosts for the cell-surface display of hemicellulolytic enzymes and optimized xylose assimilation, aiming at the development of whole-cell biocatalysts for consolidated bioprocessing of corn cob-derived hemicellulose. These modifications allowed the direct production of ethanol from non-detoxified hemicellulosic liquor obtained by hydrothermal pretreatment of corn cob, reaching an ethanol titer of 11.1 g/L corresponding to a yield of 0.328 gram per gram of potential xylose and glucose, without the need for external hydrolytic catalysts. Also, consolidated bioprocessing of pretreated corn cob was found to be more efficient for hemicellulosic ethanol production than simultaneous saccharification and fermentation with addition of commercial hemicellulases. These results show the potential of industrial S. cerevisiae strains for the design of whole-cell biocatalysts and paves the way for the development of more efficient consolidated bioprocesses for lignocellulosic biomass valorization, further decreasing environmental and economic costs.

  Cold Spring Harbor Laboratory, Jul. 2020, Biotechnology for Biofuels, 13(1) (1), 1 - 15, English

  [Refereed]

  Scientific journal


• Case study on the environmental monitoring for biological manufacturing using Time-lapse Shadow Image Analysis

  Akihiko Kondo

  Jul. 2020, Biologicals, 66, 1 - 8, English

  Scientific journal


• Base editors for simultaneous introduction of C-to-T and A-to-G mutations.

  Rina C Sakata, Soh Ishiguro, Hideto Mori, Mamoru Tanaka, Kenji Tatsuno, Hiroki Ueda, Shogo Yamamoto, Motoaki Seki, Nanami Masuyama, Keiji Nishida, Hiroshi Nishimasu, Kazuharu Arakawa, Akihiko Kondo, Osamu Nureki, Masaru Tomita, Hiroyuki Aburatani, Nozomu Yachie

  We describe base editors that combine both cytosine and adenine base-editing functions. A codon-optimized fusion of the cytosine deaminase PmCDA1, the adenosine deaminase TadA and a Cas9 nickase (Target-ACEmax) showed a high median simultaneous C-to-T and A-to-G editing activity at 47 genomic targets. On-target as well as DNA and RNA off-target activities of Target-ACEmax were similar to those of existing single-function base editors.

  Springer Science and Business Media LLC, Jul. 2020, Nature biotechnology, 38(7) (7), 865 - 869, English, International magazine

  [Refereed]

  Scientific journal


• Publisher Correction: Base editors for simultaneous introduction of C-to-T and A-to-G mutations.

  Rina C Sakata, Soh Ishiguro, Hideto Mori, Mamoru Tanaka, Kenji Tatsuno, Hiroki Ueda, Shogo Yamamoto, Motoaki Seki, Nanami Masuyama, Keiji Nishida, Hiroshi Nishimasu, Kazuharu Arakawa, Akihiko Kondo, Osamu Nureki, Masaru Tomita, Hiroyuki Aburatani, Nozomu Yachie

  An amendment to this paper has been published and can be accessed via a link at the top of the paper.

  Jul. 2020, Nature biotechnology, 38(7) (7), 901 - 901, English, International magazine

  [Refereed]


• Metabolic engineering of E. coli for improving mevalonate production to promote NADPH regeneration and enhance acetyl‐CoA supply

  Daichi Satowa, Ryosuke Fujiwara, Shogo Uchio, Mariko Nakano, Chisako Otomo, Yuuki Hirata, Takuya Matsumoto, Shuhei Noda, Tsutomu Tanaka, Akihiko Kondo

  Microbial production of mevalonate from renewable feedstock is a promising and sustainable approach for the production of value-added chemicals. We describe the metabolic engineering of Escherichia coli to enhance mevalonate production from glucose and cellobiose. First, the mevalonate-producing pathway was introduced into E. coli and the expression of the gene atoB, which encodes the gene for acetoacetyl-CoA synthetase, was increased. Then, the deletion of the pgi gene, which encodes phosphoglucose isomerase, increased the NADPH/NADP+ ratio in the cells but did not improve mevalonate production. Alternatively, to reduce flux toward the tricarboxylic acid cycle, gltA, which encodes citrate synthetase, was disrupted. The resultant strain, MGΔgltA-MV, increased levels of intracellular acetyl-CoA up to sevenfold higher than the wild-type strain. This strain produced 8.0 g/L of mevalonate from 20 g/L of glucose. We also engineered the sugar supply by displaying β-glucosidase (BGL) on the cell surface. When cellobiose was used as carbon source, the strain lacking gnd displaying BGL efficiently consumed cellobiose and produced mevalonate at 5.7 g/L. The yield of mevalonate was 0.25 g/g glucose (1 g of cellobiose corresponds to 1.1 g of glucose). These results demonstrate the feasibility of producing mevalonate from cellobiose or cellooligosaccharides using an engineered E. coli strain.

  Wiley, Jul. 2020, Biotechnology and Bioengineering, 117(7) (7), 2153 - 2164, English, International magazine

  [Refereed]

  Scientific journal


• Simultaneous increases in the levels of compatible solutes by cost-effective cultivation of Synechocystis sp. PCC 6803.

  Hiroko Iijima, Atsuko Watanabe, Haruna Sukigara, Tomokazu Shirai, Akihiko Kondo, Takashi Osanai

  Synechocystis sp. PCC 6803, a cyanobacterium widely used for basic research, is often cultivated in a synthetic medium, BG-11, in the presence of 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES) or 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]ethanesulfonic acid buffer. Owing to the high cost of HEPES buffer (96.9% of the total cost of BG-11 medium), the biotechnological application of BG-11 is limited. In this study, we cultured Synechocystis sp. PCC 6803 cells in BG-11 medium without HEPES buffer and examined the effects on the primary metabolism. Synechocystis sp. PCC 6803 cells could grow in BG-11 medium without HEPES buffer after adjusting for nitrogen sources and light intensity; the production rate reached 0.54 g cell dry weight·L-1 ·day-1 , exceeding that of commercial cyanobacteria and Synechocystis sp. PCC 6803 cells cultivated under other conditions. The exclusion of HEPES buffer markedly altered the metabolites in the central carbon metabolism; particularly, the levels of compatible solutes, such as sucrose, glucosylglycerol, and glutamate were increased. Although the accumulation of sucrose and glucosylglycerol under high salt conditions is antagonistic to each other, these metabolites accumulated simultaneously in cells grown in the cost-effective medium. Because these metabolites are used in industrial feedstocks, our results reveal the importance of medium composition for the production of metabolites using cyanobacteria.

  Wiley, Jun. 2020, Biotechnology and bioengineering, 117(6) (6), 1649 - 1660, English, International magazine

  [Refereed]

  Scientific journal


• Effective bifidogenic growth factors cyclo-Val-Leu and cyclo-Val-Ile produced by Bacillus subtilis C-3102 in the human colonic microbiota model

  Misaki Hatanaka, Hiroto Morita, Yumi Aoyagi, Kengo Sasaki, Daisuke Sasaki, Akihiko Kondo, Teppei Nakamura

  May 2020, Scientific Reports, 10, 7591, English

  [Refereed]

  Scientific journal


• High enzymatic recovery and purification of xylooligosaccharides from empty fruit bunch via nanofiltration

  Hans Wijaya, Kengo Sasaki, Prihardi Kahar, Nanik Rahmani, Euis Hermiati, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  May 2020, Processes, 8(5) (5), 619, English

  [Refereed]

  Scientific journal


• Bacillus coagulans SANK 70528 suppresses Enterobacteriaceae in the microbiota of ulcerative colitis in vitro and enhances butyrogenesis in healthy microbiota

  Kengo Sasaki, Daisuke Sasaki, Jun Inoue, Namiko Hoshi, Takayuki Maeda, Tyouichi Yamada, Akihiko Kondo

  The aim of this study was to clarify the effect of the spore-forming and lactic acid-producing probiotic strain, Bacillus coagulans SANK 70258, on human colonic microbiota of healthy subjects and ulcerative colitis patients. A model culture system was employed to construct the in vitro human colonic microbiota, to retain the bacterial species richness and simulate the patient's disordered composition, from the fecal inoculum. Bacterial 16S rRNA gene sequencing confirmed that administration of B. coagulans SANK 70258 (at an initial concentration of 4 × 107-total cells/mL) suppressed bacteria related to the family Enterobacteriaceae in the microbiota models for both healthy subjects (P = 0.016) and ulcerative colitis patients (P = 0.023). In addition, administration of B. coagulans SANK 70258 increased bacteria related to the family Lachnospiraceae (P = 0.031), thereby enhancing butyrate production (P = 0.031) in the microbiota models of healthy subjects. However, these changes were not observed in the microbiota models of ulcerative colitis patients, likely owing to the low abundance of Lachnospiraceae species. This study demonstrates the potential of B. coagulans SANK 70258 to exhibit antimicrobial activity against harmful organisms in patients with ulcerative colitis, while improving the intestinal microenvironment by increasing butyrogenesis in healthy persons. KEY POINTS: • B. coagulans SANK 70258 treatment reduced colonic Enterobacteriaceae species. • B. coagulans SANK 70258 treatment enhanced butyrogenesis in healthy individuals. • B. coagulans SANK 70258 treatment increased Lachnospiraceae in healthy persons. • B. coagulans SANK 70258 improves the colonic microenvironment in ulcerative colitis.

  May 2020, Applied Microbiology and Biotechnology, 104(9) (9), 3859 - 3867, English, International magazine

  [Refereed]

  Scientific journal


• Automatic Redirection of Carbon Flux between Glycolysis and Pentose Phosphate Pathway Using an Oxygen-Responsive Metabolic Switch in Corynebacterium glutamicum.

  Shunsuke Kobayashi, Hideo Kawaguchi, Tomokazu Shirai, Kazuaki Ninomiya, Kenji Takahashi, Akihiko Kondo, Yota Tsuge

  Controlling the carbon flux into a desired pathway is important for improving product yield in metabolic engineering. After entering a cell, glucose is channeled into glycolysis and the pentose phosphate pathway (PPP), which decreases the yield of target products whose synthesis relies on NADPH as a cofactor. Here, we demonstrate redirection of carbon flux into PPP under aerobic conditions in Corynebacterium glutamicum, achieved by replacing the promoter of glucose 6-phosphate isomerase gene (pgi) with an anaerobic-specific promoter of the lactate dehydrogenase gene (ldhA). The promoter replacement increased the split ratio of carbon flux into PPP from 39 to 83% under aerobic conditions. The titer, yield, and production rate of 1,5-diaminopentane, whose synthesis requires NADPH as a cofactor, were increased by 4.6-, 4.4-, and 2.6-fold, respectively. This is the largest improvement in the production of 1,5-diaminopentane or its precursor, lysine, reported to date. After aerobic cell growth, pgi expression was automatically induced under anaerobic conditions, altering the carbon flux from PPP to glycolysis, to produce succinate in a single metabolically engineered strain. Such an automatic redirection of metabolic pathway using an oxygen-responsive switch enables two-stage fermentation for efficient production of two different compounds by a single strain, potentially reducing the production costs and time for practical applications.

  Apr. 2020, ACS synthetic biology, 9(4) (4), 814 - 826, English, International magazine

  [Refereed]

  Scientific journal


• Concentration of Lipase from Aspergillus oryzae Expressing Fusarium heterosporum by Nanofiltration to Enhance Transesterification

  Hans Wijaya, Kengo Sasaki, Prihardi Kahar, Emmanuel Quayson, Nova Rachmadona, Jerome Amoah, Shinji Hama, Chiaki Ogino, Akihiko Kondo

  Apr. 2020, Processes, 8(4) (4), 450, English

  [Refereed]

  Scientific journal


• Lipase-catalyzed ethanolysis for biodiesel production of untreated palm oil mill effluent

  Nova Rachmadona, Jerome Amoah, Emmanuel Quayson, Shinji Hama, Ayumi Yoshida, Akihiko Kondo, Chiaki Ogino

  Mar. 2020, SUSTAINABLE ENERGY & FUELS, 4(3) (3), 1105 - 1111, English

  Scientific journal


• Biodiesel-mediated biodiesel production: A recombinant Fusarium heterosporum lipase-catalyzed transesterification of crude plant oils

  Akihiko Kondo

  Mar. 2020, Fuel Processing Technology, 199, 106278, English

  [Refereed]

  Scientific journal


• Malic Enzyme Facilitates d-Lactate Production through Increased Pyruvate Supply during Anoxic Dark Fermentation in Synechocystis sp. PCC 6803.

  Ryota Hidese, Mami Matsuda, Takashi Osanai, Tomohisa Hasunuma, Akihiko Kondo

  d-Lactate is one of the most valuable compounds for manufacturing biobased polymers. Here, we have investigated the significance of endogenous malate dehydrogenase (decarboxylating) (malic enzyme, ME), which catalyzes the oxidative decarboxylation of malate to pyruvate, in d-lactate biosynthesis in the cyanobacterium Synechocystis sp. PCC6803. d-Lactate levels were increased by 2-fold in ME-overexpressing strains, while levels in ME-deficient strains were almost equivalent to those in the host strain. Dynamic metabolomics revealed that overexpression of ME led to increased turnover rates in malate and pyruvate metabolism; in contrast, deletion of ME resulted in increased pool sizes of glycolytic intermediates, probably due to sequential feedback inhibition, initially triggered by malate accumulation. Finally, both the loss of the acetate kinase gene and overexpression of endogenous d-lactate dehydrogenase, concurrent with ME overexpression, resulted in the highest production of d-lactate (26.6 g/L) with an initial cell concentration of 75 g-DCW/L after 72 h fermentation.

  Feb. 2020, ACS synthetic biology, 9(2) (2), 260 - 268, English, International magazine

  [Refereed]


• Exploration and Evaluation of Machine Learning-Based Models for Predicting Enzymatic Reactions

  Naoki Watanabe, Masahiro Murata, Teppei Ogawa, Christopher J Vavricka, Akihiko Kondo, Chiaki Ogino, Michihiro Araki

  Feb. 2020, Journal of Chemical Information and Modeling, 60(3) (3), 1833 - 1843, English

  [Refereed]

  Scientific journal


• Novel strategy for anchorage position control of GPI-attached proteins in the yeast cell wall using different GPI-anchoring domains

  Akihiko Kondo

  Jan. 2020, Metabolic Engineering, 57, 110 - 117, English

  [Refereed]

  Scientific journal


• Metabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose-xylose co-substrate (vol 14, pg 135, 2020)

  Akihiko Kondo

  2020, Nature Communications

  Scientific journal


• Verbal function recovery in a postoperative case with epileptic encephalopathy

  Akihiko Kondo

  2020, Pediatrics International

  Scientific journal


• Retained absolute pitch after selective amygdalohippocampectomy

  Akihiko Kondo

  2020, Epilepsy & Behavior Reports

  Scientific journal


• Metabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose-xylose co-substrate

  Akihiko Kondo

  Jan. 2020, Nature Communications, 11, 279, English

  [Refereed]

  Scientific journal


• Dynamic Metabolomics for Engineering Biology: Accelerating Learning Cycles for Bioproduction

  Christopher J. Vavricka, Tomohisa Hasunuma, Akihiko Kondo

  Jan. 2020, Trends in Biotechnology, 38(1) (1), 68 - 82, English

  [Refereed]

  Scientific journal


• n-Butylamine production from glucose using a transaminase-mediated synthetic pathway in Escherichia coli.

  Takuya Matsumoto, Yuki Mori, Tsutomu Tanaka, Akihiko Kondo

  Bioamination methods using microorganisms have attracted much attention because of the increasing demand for environmentally friendly bioprocesses. n-Butylamine production from glucose in Escherichia coli was demonstrated in this study, which has never been reported because of the absence of n-butylamine-producing pathway in nature. We focused on a transaminase-mediated cascade for bioamination from an alcohol or aldehyde. The cascade can convert an alcohol or an aldehyde to the corresponding amine with l-alanine as an amine donor. Here, n-butyraldehyde, which is a metabolic intermediate in the n-butanol producing pathway, is a potential intermediate for producing n-butylamine using this cascade. Hence, the n-butanol-producing pathway and the transaminase-mediated cascade were combined into a synthetic metabolic pathway for producing n-butylamine from glucose. Firstly, we demonstrated the conversion of n-butanol to n-butylamine using a three enzyme-mediated cascade. n-Butanol was successfully converted to n-butylamine in 92% yield in the presence of l-alanine and ammonium chloride. Then, the n-butanol-producing pathway and transaminase-mediated cascade were introduced into E. coli. Using this system, n-butylamine was successfully produced from glucose as a carbon source at a concentration of 53.2 mg L-1 after 96 h cultivation using a ppc (phosphoenolpyruvate carboxylase)-deficient strain. To the best of our knowledge, this is the first report of the direct production of n-butylamine from glucose, and may provide a starting point for the development of microbial methods to produce other bioamines.

  Jan. 2020, Journal of bioscience and bioengineering, 129(1) (1), 99 - 103, English, Domestic magazine

  [Refereed]

  Scientific journal


• Fermentation of pigment-extracted microalgal residue using yeast cell-surface display: direct high-density ethanol production with competitive life cycle impacts

  Akihiko Kondo

  2020, Green Chemistry, 22(1) (1), 153 - 162, English

  [Refereed]

  Scientific journal


• Single-Stage Astaxanthin Production Enhances the Nonmevalonate Pathway and Photosynthetic Central Metabolism in Synechococcus sp. PCC 7002

  Tomohisa Hasunuma, Ayako Takaki, Mami Matsuda, Yuichi Kato, Christopher J. Vavricka, Akihiko Kondo

  Dec. 2019, ACS Synthetic Biology, 8, 2701 - 2709, English

  [Refereed]

  Scientific journal


• 5-Aminolevulinic acid fermentation using engineered Saccharomyces cerevisiae

  Kiyotaka Y. Hara, Masaru Saito, Hiroko Kato, Kana Morikawa, Hiroshi Kikukawa, Hironari Nomura, Takanori Fujimoto, Yoko Hirono-Hara, Shigeyuki Watanabe, Kengo Kanamaru, Akihiko Kondo

  Springer Science and Business Media LLC, Dec. 2019, Microbial Cell Factories, 18(1) (1)

  Scientific journal


• Production of 1,2,4-butanetriol from xylose by Saccharomyces cerevisiae through Fe metabolic engineering

  Akihiko Kondo

  Dec. 2019, Metabolic Engineering, 56, 17 - 27, English

  [Refereed]

  Scientific journal


• Mechanism-based tuning of insect 3,4-dihydroxyphenylacetaldehyde synthase for synthetic bioproduction of benzylisoquinoline alkaloids

  Christopher J. Vavricka, Takanobu Yoshida, Yuki Kuriya, Shunsuke Takahashi, Teppei Ogawa, Fumie Ono, Kazuko Agari, Hiromasa Kiyota, Jianyong Li, Jun Ishii, Kenji Tsuge, Hiromichi Minami, Michihiro Araki, Tomohisa Hasunuma, Akihiko Kondo

  Dec. 2019, Nature Communications, 10(1) (1)

  [Refereed]

  Scientific journal


• Short-Term Temporal Metabolic Behavior in Halophilic Cyanobacterium Synechococcus sp. Strain PCC 7002 after Salt Shock

  Akihiko Kondo

  Dec. 2019, Metabolites, 9(12) (12), 297, English

  [Refereed]

  Scientific journal


• Bifidogenic and butyrogenic effects of young barely leaf extract in an in vitro human colonic microbiota model

  Akihiko Kondo

  Nov. 2019, AMB Express, 9, 182, English

  [Refereed]

  Scientific journal


• Repression of mitochondrial metabolism for cytosolic pyruvate-derived chemical production in Saccharomyces cerevisiae.

  Keisuke Morita, Fumio Matsuda, Koji Okamoto, Jun Ishii, Akihiko Kondo, Hiroshi Shimizu

  BACKGROUND: Saccharomyces cerevisiae is a suitable host for the industrial production of pyruvate-derived chemicals such as ethanol and 2,3-butanediol (23BD). For the improvement of the productivity of these chemicals, it is essential to suppress the unnecessary pyruvate consumption in S. cerevisiae to redirect the metabolic flux toward the target chemical production. In this study, mitochondrial pyruvate transporter gene (MPC1) or the essential gene for mitophagy (ATG32) was knocked-out to repress the mitochondrial metabolism and improve the production of pyruvate-derived chemical in S. cerevisiae. RESULTS: The growth rates of both aforementioned strains were 1.6-fold higher than that of the control strain. 13C-metabolic flux analysis revealed that both strains presented similar flux distributions and successfully decreased the tricarboxylic acid cycle fluxes by 50% compared to the control strain. Nevertheless, the intracellular metabolite pool sizes were completely different, suggesting distinct metabolic effects of gene knockouts in both strains. This difference was also observed in the test-tube culture for 23BD production. Knockout of ATG32 revealed a 23.6-fold increase in 23BD titer (557.0 ± 20.6 mg/L) compared to the control strain (23.5 ± 12.8 mg/L), whereas the knockout of MPC1 revealed only 14.3-fold increase (336.4 ± 113.5 mg/L). Further investigation using the anaerobic high-density fermentation test revealed that the MPC1 knockout was more effective for ethanol production than the 23BD production. CONCLUSION: These results suggest that the engineering of the mitochondrial transporters and membrane dynamics were effective in controlling the mitochondrial metabolism to improve the productivities of chemicals in yeast cytosol.

  Oct. 2019, Microbial cell factories, 18(1) (1), 177 - 177, English, International magazine

  [Refereed]

  Scientific journal


• Butyryl-CoA:acetate CoA-transferase gene associated with the genus Roseburia is decreased in the gut microbiota of Japanese patients with ulcerative colitis

  Ryohei Shinohara, Kengo Sasaki, Jun Inoue, Namiko Hoshi, Itsuko Fukuda, Daisuke Sasaki, Akihiko Kondo, Ro Osawa

  Microbial production of butyrate is impaired in patients with ulcerative colitis (UC); however, this inhibition is not well understood in Japanese UC patients. Therefore, we quantitatively analyzed genes encoding butyryl-CoA:acetate CoA-transferase (but) and butyrate kinase (buk) in the gut microbiota of Japanese patients with UC and healthy volunteers (HVs). But showed higher levels than buk. Moreover, patients with UC showed significantly decreased levels of but associated with Roseburia sp./Eubacterium rectale compared with HVs. But, which is associated with Faecalibacterium sp., was maintained in patients with UC, with an unchanged relative abundance of Faecalibacterium sp. microorganisms in patients with UC compared with HVs.

  Oct. 2019, Bioscience of Microbiota, Food and Health, 38(4) (4), 159 - 163, English, Domestic magazine

  [Refereed]

  Scientific journal


• Metabolic engineering to improve 1,5‐diaminopentane production from cellobiose using β‐glucosidase‐secreting Corynebacterium glutamicum

  Rena Matsuura, Mayumi Kishida, Rie Konishi, Yuuki Hirata, Noriko Adachi, Shota Segawa, Kenta Imao, Tsutomu Tanaka, Akihiko Kondo

  Oct. 2019, Biotechnology and Bioengineering, 116(10) (10), 2640 - 2651, English

  [Refereed]

  Scientific journal


• A novel process for the mixotrophic production of lutein with Chlorella sorokiniana MB-1-M12 using aquaculture wastewater

  Jih-Heng Chen, Yuichi Kato, Mami Matsuda, Chun-Yen Chen, Dillirani Nagarajan, Tomohisa Hasunuma, Akihiko Kondo, Cheng-Di Dong, Duu-Jong Lee, Jo-Shu Chang

  In this study, microalgal cultivation was applied as a feasible strategy for treating shrimp culture wastewater (SCW) from a shrimp farm in southern Tainan. Chlorella sorokiniana MB-1-M12 was first grown on BG-11 medium with 0.5% salinity, obtaining a biomass concentration and productivity of 4.35 g/L and 1.56 g/L/d, respectively. When 80% of BG-11 nutrients were added to 75% strength SCW, lutein content and productivity increased to 5.19 mg/g and 5.55 mg/L/d, respectively. A novel operation strategy involving periodic exchange of freshwater and SCW was designed for semi-continuous cultivation of MB-1-M12 strain for optimal biomass and lutein production. The average biomass concentration, productivity, lutein content, and productivity were 3.5 g/L, 1.3 g/L/d, 3.89 mg/g and 5.0 mg/L/d, respectively. Although microalgae have been considered as an alternative natural source of lutein, this work is among the earliest reports describing lutein production from microalgae cultivated with wastewater via a circular economy concept.

  Oct. 2019, Bioresource Technology, 290, 121786 - 121786, English, International magazine

  [Refereed]

  Scientific journal


• Complete Genome Sequence of Rhodobacter sphaeroides Strain HJ, a Purple Nonsulfur Bacterium with the Ability To Produce High Levels of Hydrogen from Acetate

  Akihiko Kondo

  Sep. 2019, Microbiology Resource Announcements, 8(37) (37), e00652-19, English

  [Refereed]

  Scientific journal


• Co-fermentation of xylose and glucose from ionic liquid pretreated sugar cane bagasse for bioethanol production using engineered xylose assimilating yeast

  Akihiko Kondo

  Sep. 2019, Biomass and Bioenergy, 128, English

  [Refereed]

  Scientific journal


• High cell density cultivation of Lipomyces starkeyi for achieving highly efficient lipid production from sugar under low C/N ratio

  Rezky Lastinov Amza, Prihardi Kahar, Ario Betha Juanssilfero, Nao Miyamoto, Hiromi Otsuka, Chie Kihira, Chiaki Ogino, Akihiko Kondo

  Sep. 2019, Biochemical Engineering Journal, 149(15) (15), 107236, English

  [Refereed]

  Scientific journal


• Combined cell surface display of β-D-glucosidase (BGL), maltose transporter (MAL11) and overexpression of cytosolic xylose reductase (XR) in Saccharomyces cerevisiae enhance cellobiose/xylose coutilization for xylitol bio-production from lignocellulosic b

  Gregory G, Y. Guirimand, Takahiro Bamba, Mami Matsuda, Kentaro Inokuma, Kenta Morita, Yuki Kitada, Yuma Kobayashi, Takahiro Yukawa, Kengo Sasaki, Chiaki Ogino, Tomohisa Hasunuma, Akihiko Kondo

  Xylitol is a highly valuable commodity chemical used extensively in the food and pharmaceutical industries. The production of xylitol from d-xylose involves a costly and polluting catalytic hydrogenation process. Biotechnological production from lignocellulosic biomass by micro-organisms like yeasts is a promising option. In this study, xylitol is produced from lignocellulosic biomass by a recombinant strain of Saccharomyces cerevisiae (S. cerevisiae) (YPH499-SsXR-AaBGL) expressing cytosolic xylose reductase (Scheffersomyces stipitis xylose reductase [SsXR]), along with a β-d-glucosidase (Aspergillus aculeatus β-glucosidase 1 [AaBGL]) displayed on the cell surface. The simultaneous cofermentation of cellobiose/xylose by this strain leads to an ≈2.5-fold increase in Yxylitol/xylose (=0.54) compared to the use of a glucose/xylose mixture as a substrate. Further improvement in the xylose uptake by the cell is achieved by a broad evaluation of several homologous and heterologous transporters. Homologous maltose transporter (ScMAL11) shows the best performance in xylose transport and is used to generate the strain YPH499-XR-ScMAL11-BGL with a significantly improved xylitol production capacity from cellobiose/xylose coutilization. This report constitutes a promising proof of concept to further scale up the biorefinery industrial production of xylitol from lignocellulose by combining cell surface and metabolic engineering in S. cerevisiae.

  Sep. 2019, Biotechnology Journal, 14(9) (9), 1800704, English, International magazine

  [Refereed]

  Scientific journal


• Effect of Resistant Starch on the Gut Microbiota and Its Metabolites in Patients with Coronary Artery Disease.

  Naofumi Yoshida, Kengo Sasaki, Daisuke Sasaki, Tomoya Yamashita, Hajime Fukuda, Tomohiro Hayashi, Tokiko Tabata, Ro Osawa, Ken-Ichi Hirata, Akihiko Kondo

  AIM: Bacteroides vulgatus and B. dorei have a protective effect against atherosclerosis, suggesting that expansion of these species in the gut microbiota could help patients with coronary artery disease (CAD). This study aimed to investigate the effect of resistant starch (RS) on the gut microbiota and its metabolites in fecal sample cultures from patients with CAD and individuals without CAD, using a single-batch fermentation system. METHODS: Fecal samples from 11 patients with CAD and 10 individuals without CAD were fermented for 30 h with or without RS in the Kobe University Human Intestinal Microbiota Model (KUHIMM). Gut microbiota and the abundance of B. vulgatus and B. dorei were analyzed using 16S ribosomal ribonucleic acid (rRNA) gene sequencing and the quantitative polymerase chain reaction. Short-chain fatty acids were analyzed using high-performance liquid chromatography. RESULTS: Gut microbial analysis showed significantly lower levels of B. vulgatus and B. dorei in the original fecal samples from patients with CAD, which was simulated after 30 h of fermentation in the KUHIMM. Although RS significantly increased the absolute numbers of B. vulgatus and B. dorei, and butyrate levels in CAD fecal sample cultures, the numbers varied among each patient. CONCLUSIONS: The effect of RS on gut microbiota and its metabolites in the KUHIMM varied between CAD and non-CAD fecal sample cultures. The KUHIMM may be useful for preclinical evaluations of the effects of RS on the gut microbiota and its metabolites.

  Aug. 2019, Journal of atherosclerosis and thrombosis, 26(8) (8), 705 - 719, English, Domestic magazine

  [Refereed]

  Scientific journal


• Day/Night Separation of Oxygenic Energy Metabolism and Nuclear DNA Replication in the Unicellular Red Alga Cyanidioschyzon merolae

  Akihiko Kondo

  Jul. 2019, mBio, 10(4) (4), e00833-19, English

  [Refereed]

  Scientific journal


• Reconstruction of metabolic pathway for isobutanol production in Escherichia coli

  Shuhei Noda, Yutaro Mori, Sachiko Oyama, Akihiko Kondo, Michihiro Araki, Tomokazu Shirai

  BACKGROUND: The microbial production of useful fuels and chemicals has been widely studied. In several cases, glucose is used as the raw material, and almost all microbes adopt the Embden-Meyerhof (EM) pathway to degrade glucose into compounds of interest. Recently, the Entner-Doudoroff (ED) pathway has been gaining attention as an alternative strategy for microbial production. RESULTS: In the present study, we attempted to apply the ED pathway for isobutanol production in Escherichia coli because of the complete redox balance involved. First, we generated ED pathway-dependent isobutanol-producing E. coli. Thereafter, the inactivation of the genes concerning organic acids as the byproducts was performed to improve the carbon flux to isobutanol from glucose. Finally, the expression of the genes concerning the ED pathway was modified. CONCLUSIONS: The optimized isobutanol-producing E. coli produced 15.0 g/L of isobutanol as the final titer, and the yield from glucose was 0.37 g/g (g-glucose/g-isobutanol).

  Jul. 2019, Microbial Cell Factories, 18(1) (1), 124 - 124, English, International magazine

  [Refereed]

  Scientific journal


• Lipid production by Lipomyces starkeyi using sap squeezed from felled old oil palm trunks

  Ario Betha Juanssilfero, Prihardi Kahar, Lastinov Amza Rezky, Yopi, Kumar Sudesh, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  The ability of oleaginous yeast Lipomyces starkeyi to efficiently produce lipids when cultivated on sap extracted from felled oil palm trunk (OPT) as a novel inexpensive renewable carbon source was evaluated. OPT sap was found to contain approximately 98 g/L glucose and 32 g/L fructose. Batch fermentations were performed using three different OPT sap medium conditions: regular sap, enriched sap, and enriched sap at pH 5.0. Under all sap medium conditions, the cell biomass and lipid production achieved were approximately 30 g/L and 60% (w/w), respectively. L. starkeyi tolerated acidified medium (initial pH ≈ 3) and produced considerable amounts of ethanol as well as xylitol as by-products. The fatty acid profile of L. starkeyi was remarkably similar to that of palm oil, one of the most common vegetable oil feedstock used in biodiesel production with oleic acid as the major fatty acid followed by palmitic, stearic and linoleic acids.

  Jun. 2019, Journal of Bioscience and Bioengineering, 127(6) (6), 726 - 731, English, Domestic magazine

  [Refereed]

  Scientific journal


• Versatility of a dilute acid/butanol pretreatment investigated on various lignocellulosic biomasses to produce lignin, monosaccharides and cellulose in distinct phases

  Quentin Schmetz, Hiroshi Teramura, Kenta Morita, Tomoko Oshima, Aurore Richel, Chiaki Ogino, Akihiko Kondo

  Jun. 2019, ACS Sustainable Chemistry & Engineering, 7(13) (13), 11069 - 11079, English

  [Refereed]

  Scientific journal


• Bioenergy and Biorefinery: feedstock, biotechnological conversion and products

  Jerome Amoah, Prihardi Kahar, Chiaki Ogino, Akihiko Kondo

  Biorefinery has been suggested to provide relevant substitutes to a number of fossil products. Feedstocks and conversion technologies have, however, been the bottleneck to the realization of this concept. Herein, feedstocks and bioconversion technologies under biorefinery have been reviewed. Over the last decade, research has shown possibilities of generating tens of new products but only few industrial implementations. This is partly associated with low production yields and poor cost-competitiveness. This review addresses the technical barriers associated with the conversion of emerging feedstocks into chemicals and bioenergy platforms and summarizes the developed biotechnological approaches including advances in metabolic engineering. This summary further suggests possible future advances that would expand the portfolio of biorefinery and speed up the realization of biofuels and biochemicals.

  Jun. 2019, Biotechnology Journal, 14(6) (6), 1800494, English, International magazine

  [Refereed]

  Scientific journal


• Enhanced phenyllactic acid production in Escherichia coli via oxygen limitation and shikimate pathway gene expression

  Hideo Kawaguchi, Hiroki Miyagawa, Sachiko Nakamura‐Tsuruta, Naoki Takaya, Chiaki Ogino, Akihiko Kondo

  3-Phenyllactic acid (PhLA) is useful as a start-up material in the pharmaceutical and biorefinery industries. To enhance the production of PhLA from glucose using recombinant Escherichia coli, the effects of glucose concentration and oxygen limitation on PhLA production are assessed in a fed-batch system using dissolved oxygen (DO)-stat method. The highest titer of PhLA (7.3 g L-1 ) is observed with a high concentration of glucose and under oxygen-limited conditions (DO = 0 ppm). Under oxygen limitation, cell growth and the formation of acetate and l-phenylalanine (Phe) by-products after 72 h of cultivation are reduced by 30%, 70%, and 81%, respectively, as compared to that under high DO conditions (DO = 5 ppm). Gene expression levels are compared between low and high DO conditions by quantitative polymerase chain reaction (qPCR) analysis. Several genes in the glycolysis (gapA and pykA), pentose phosphate (tktA), and early shikimate pathways for PhLA biosynthesis (aroF, aroG, and aroH) are upregulated under oxygen limitation. The results suggest that oxygen limitation affects metabolism in the shikimate pathway at both metabolic and transcriptional levels and that controlling the DO level is critical for enhanced production of a variety of aromatic compounds through the shikimate pathway.

  Jun. 2019, Biotechnology Journal, 14(6) (6), 1800478, English, International magazine

  [Refereed]

  Scientific journal


• Author Correction: Mechanism-based tuning of insect 3,4-dihydroxyphenylacetaldehyde synthase for synthetic bioproduction of benzylisoquinoline alkaloids.

  Christopher J Vavricka, Takanobu Yoshida, Yuki Kuriya, Shunsuke Takahashi, Teppei Ogawa, Fumie Ono, Kazuko Agari, Hiromasa Kiyota, Jianyong Li, Jun Ishii, Kenji Tsuge, Hiromichi Minami, Michihiro Araki, Tomohisa Hasunuma, Akihiko Kondo

  In the original version of this Article, the abbreviation of 3,4-dihydroxyphenylacetaldehyde synthase presented in the first paragraph of the Discussion section was given incorrectly as DYPAA. The correct abbreviation for this enzyme is DHPAAS. This error has been corrected in both the PDF and HTML versions of the Article.

  May 2019, Nature communications, 10(1) (1), 2336 - 2336, English, International magazine

  [Refereed]

  Scientific journal


• Cell-surface display technology and metabolic engineering of Saccharomyces cerevisiae for enhancing xylitol production from woody biomass

  Akihiko Kondo

  May 2019, Green Chemistry, 21, 1795 - 1808, English

  [Refereed]

  Scientific journal


• Construction of a model culture system of human colonic microbiota to detect decreased Lachnospiraceae abundance and butyrogenesis in the feces of ulcerative colitis patients

  Kengo Sasaki, Jun Inoue, Daisuke Sasaki, Namiko Hoshi, Tomokazu Shirai, Itsuko Fukuda, Takeshi Azuma, Akihiko Kondo, Ro Osawa

  Compositional alteration of the gut microbiota is associated with ulcerative colitis (UC). Here, a model culture system is established for the in vitro human colonic microbiota of UC, which will be helpful for determining medical interventions. 16S ribosomal RNA sequencing confirms that UC models are successfully developed from fecal inoculum and retain the bacterial species biodiversity of UC feces. The UC models closely reproduce the microbial components and successfully preserve distinct clusters from the healthy subjects (HS), as observed in the feces. The relative abundance of bacteria belonging to the family Lachnospiraceae significantly decreases in the UC models compared to that in HS, as observed in the feces. The system detects significantly lower butyrogenesis in the UC models than that in HS, correlating with the decreased abundance of Lachnospiraceae. Interestingly, the relative abundance of Lachnospiraceae does not correlate with disease activity (defined as partial Mayo score), suggesting that Lachnospiraceae persists in UC patients at a decreased level, irrespective of the alteration in disease activity. Moreover, the system shows that administration of Clostridium butyricum MIYAIRI restores butyrogenesis in the UC model. Hence, the model detects deregulation in the intestinal environment in UC patients and may be useful for simulating the effect of probiotics.

  May 2019, Biotechnology Journal, 14(5) (5), 1800555, English, International magazine

  [Refereed]

  Scientific journal


• Building a global alliance of biofoundries

  HILLSON Nathan, CADDICK Mark, CAI Yizhi, CARRASCO Jose A, CHANG Matthew Wook, CURACH Natalie C, BELL David J, Le FEUVRE Rosalind, FRIEDMAN Douglas C, FU Xiongfei, GOLD Nicholas D, HERRGARD Markus J, HOLOWKO Maciej B, JOHNSON James R, JOHNSON Richard A, KEASLING Jay D, KITNEY Richard I, KONDO Akihiko, LIU Chenli, MARTIN Vincent JJ, MENOLASCINA Filippo, OGINO Chiaki, PATRON Nicola J, PAVAN Marilene, POH Chueh Loo, PRETORIUS Lsak S, ROSSER Susan J, SCRUTTON Nigel S, STORCH Marko, TEKOTTE Hille, TRAVNIK Evelyn, VIV\CKERS Claudia E, YEW Wen Shan, YUAN Yingjin, ZHAO Huimin, FREEMONT Paul S

  May 2019, Nature Communications, 10(1) (1), 2040 - 2040, English, International magazine

  [Refereed]

  Scientific journal


• Enhancing lutein production with mixotrophic cultivation of Chlorella sorokiniana MB-1-M12 using different bioprocess operation strategies

  Jih-Heng Chen, Chun-Yen Chen, Tomohisa Hasunuma, Akihiko Kondo, Chien-Hsiang Chang, I-Son Ng, Jo-Shu Chang

  Apr. 2019, Bioresource Technology, 278, 17 - 25, English

  [Refereed]

  Scientific journal


• Targeted Base Editing with CRISPR-Deaminase in Tomato

  Akihiko Kondo

  Apr. 2019, Methods in Molecular Biology, 1917, 297 - 307, English

  [Refereed]

  Scientific journal


• Heterologous production of free dihomo-γ-linolenic acid by Aspergillus oryzae and its extracellular release via surfactant supplementation

  Koichi Tamano, Robert Sidney Cox, Kenji Tsuge, Ai Miura, Ayano Itoh, Jun Ishii, Tomohiro Tamura, Akihiko Kondo, Masayuki Machida

  Free dihomo-γ-linolenic acid (DGLA) and its desaturated form, free arachidonic acid (ARA) are polyunsaturated free fatty acids (FFAs). They are useful raw materials to produce eicosanoid pharmaceuticals. In this study, we aimed at their production by the oleaginous filamentous fungus Aspergillus oryzae via metabolic engineering. Three genes encoding enzymes involved in the synthesis of DGLA and ARA, were isolated from the filamentous fungus Mortierella alpina that produces ARA in a triacylglycerol form. These genes were concatenated to promoters and terminators of highly expressed genes of A. oryzae, and the concatenated DNA fragments were further concatenated with each other to generate a single DNA fragment in the form of a biosynthetic gene cluster. By homologous recombination, the resulting DNA fragment was integrated to the chromosome of the A. oryzae acyl-CoA synthetase gene disruptant whose FFA productivity was enhanced at 9.2-fold more than the wild-type strain. The DNA-integrated disruptant produced free DGLA but did not produce free ARA. Thus, focusing on free DGLA, after removal of the gene for converting DGLA to ARA, the constructed strain produced free DGLA at 145 mg/l for 5 d. Also, by supplementing Triton X-100 surfactant at 1% to the culture, over 80% of free DGLA was released from cells without inhibiting the growth. Consequently, the constructed strain will be useful for attempting production of free DGLA-derived eicosanoids because it bypasses excision of free DGLA from triacylglycerols by lipase. To our knowledge, this is the first report on microbial production of free DGLA and its extracellular release.

  Apr. 2019, Journal of Bioscience and Bioengineering, 127(4) (4), 451 - 457, English, Domestic magazine

  [Refereed]

  Scientific journal


• Modified expression of multi-cellulases in a filamentous fungus Aspergillus oryzae

  Satoshi Wakai, Nanami Nakashima, Chiaki Ogino, Hiroko Tsutsumi, Yoji Hata, Akihiko Kondo

  Aspergillus oryzae, a filamentous fungus, can secrete large amounts of enzymes extracellularly. We constructed a genetically engineered A. oryzae that simultaneously produced cellobiohydrolase, endoglucanase, and β-glucosidase by integrating multiple copies of the genes encoding these cellulases into fungal chromosomes. The resulting strain possessed 5-16 copies of each cellulase gene within the chromosome and showed approximately 10-fold higher activity versus single integration strains. Copy number polymorphisms were attributed to differences in flanking region sequence for the integrated gene fragments. Furthermore, we found that the P-sodM/T-glaB set demonstrated the strongest transcription levels per gene copy number. We therefore modified promoter/terminator set and cellulase gene combinations based on this polymorphism and transcription level data, with the resulting transformant showing 40-fold higher cellulolytic activity versus the single integration strain. This designed expression method could be useful for the overexpression of multiple enzymes and pathway flux control-mediated metabolic engineering in A. oryzae.

  Mar. 2019, Bioresource Technology, 276, 146 - 153, English, International magazine

  [Refereed]

  Scientific journal


• Valorization of activated carbon as a reusable matrix for the immobilization of Aspergillus oryzae whole-cells expressing Fusarium heterosporum lipase toward biodiesel synthesis

  Emmanuel Quayson, Jerome Amoah, Shinji Hama, Ayumi Yoshida, Kenta Morita, Akihiko Kondo, Chiaki Ogino

  Mar. 2019, ACS Sustainable Chemistry & Engineering, 7(5) (5), 5010 - 5017, English

  [Refereed]

  Scientific journal


• Increased flux in acetyl-CoA synthetic pathway and TCA cycle of Kluyveromyces marxianus under respiratory conditions

  SAKIHAMA Yuri, HIDESE Ryota, HASUNUMA Tomohisa, KONDO Akihiko

  Yeasts are extremely useful, not only for fermentation but also for a wide spectrum of fuel and chemical productions. We analyzed the overall metabolic turnover and transcript dynamics in glycolysis and the TCA cycle, revealing the difference in adaptive pyruvate metabolic response between a Crabtree-negative species, Kluyveromyces marxianus, and a Crabtree-positive species, Saccharomyces cerevisiae, during aerobic growth. Pyruvate metabolism was inclined toward ethanol production under aerobic conditions in S. cerevisiae, while increased transcript abundances of the genes involved in ethanol metabolism and those encoding pyruvate dehydrogenase were seen in K. marxianus, indicating the augmentation of acetyl-CoA synthesis. Furthermore, different metabolic turnover in the TCA cycle was observed in the two species: malate and fumarate production in S. cerevisiae was higher than in K. marxianus, irrespective of aeration; however, fluxes of both the reductive and oxidative TCA cycles were enhanced in K. marxianus by aeration, implying both the cycles contribute to efficient electron flux without producing ethanol. Additionally, decreased hexokinase activity under aerobic conditions is expected to be important for maintenance of suitable carbon flux. These findings demonstrate differences in the key metabolic trait of yeasts employing respiration or fermentation, and provide important insight into the metabolic engineering of yeasts.

  Mar. 2019, Scientific Reports, 9(1) (1), 5319 - 5319, English, International magazine

  [Refereed]

  Scientific journal


• Efficient and supplementary enzyme cocktail from Actinobacteria and plant biomass induction

  Musashi Takenaka, Lee Jae Min, PRIHARDI KAHAR, Chiaki Ogino, Akihiko Kondo

  Actinobacteria plays a key role in the cycling of organic matter in soils. They secret biomass-degrading enzymes that allow it to produce the unique metabolites that originate in plant biomass. Although past studies have focused on these unique metabolites, a large-scale screening of Actinobacteria is yet to be reported to focus on their biomass-degrading ability. In the present study, a rapid and simple method is constructed for a large-scale screening, and the novel resources that form the plant biomass-degrading enzyme cocktail are identified from 850 isolates of Actinobacteria. As a result, Nonomuraea fastidiosa secretes a biomass degrading enzyme cocktail with the highest enzyme titer, although cellulase activities are lower than a commercially available enzyme. So the rich accessory enzymes are suggested to contribute to the high enzyme titer for a pretreated bagasse with a synergistic effect. Additionally, an optimized cultivation method of biomass induction caused to produce the improved enzyme cocktail indicated strong enzyme titers and a strong synergistic effect. Therefore, the novel enzyme cocktails are selected via the optimized method for large-scale screening, and then the enzyme cocktail can be improved via the optimized production with biomass-induction.

  Mar. 2019, Biotechnology Journal, 14(3) (3), 1700744, English, International magazine

  [Refereed]

  Scientific journal


• Adaptation of light-harvesting functions of unicellular green algae to different light qualities.

  Yoshifumi Ueno, Shimpei Aikawa, Akihiko Kondo, Seiji Akimoto

  Oxygenic photosynthetic organisms perform photosynthesis efficiently by distributing captured light energy to photosystems (PSs) at an appropriate balance. Maintaining photosynthetic efficiency under changing light conditions requires modification of light-harvesting and energy-transfer processes. In the current study, we examined how green algae regulate their light-harvesting functions in response to different light qualities. We measured low-temperature time-resolved fluorescence spectra of unicellular green algae Chlamydomonas reinhardtii and Chlorella variabilis cells grown under different light qualities. By observing the delayed fluorescence spectra, we demonstrated that both types of green algae primarily modified the associations between light-harvesting chlorophyll protein complexes (LHCs) and PSs (PSII and PSI). Under blue light, Chlamydomonas transferred more energy from LHC to chlorophyll (Chl) located far from the PSII reaction center, while energy was transferred from LHC to PSI via different energy-transfer pathways in Chlorella. Under green light, both green algae exhibited enhanced energy transfer from LHCs to both PSs. Red light induced fluorescence quenching within PSs in Chlamydomonas and LHCs in Chlorella. In Chlorella, energy transfer from PSII to PSI appears to play an important role in balancing excitation between PSII and PSI.

  Mar. 2019, Photosynthesis research, 139(1-3) (1-3), 145 - 154, English, International magazine

  [Refereed]

  Scientific journal


• Sustainable production of glutathione from lignocellulose-derived sugars using engineered Saccharomyces cerevisiae

  Akihiko Kondo

  Feb. 2019, Applied Microbiology and Biotechnology, 103(3) (3), 1243 - 1254, English

  [Refereed]

  Scientific journal


• Light/dark cycling causes delayed lipid accumulation and increased photoperiod-based biomass yield by altering metabolic flux in oleaginous Chlamydomonas sp.

  Yuichi KATO, Yusuke FUJIHARA, Christopher J. VAVRICKA, Jo-Shu CHANG, Tomohisa HASUMUMA, Akihiko KONDO

  BACKGROUND: Light/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on cellular lipid production has not been clearly established. The general aim of this study was to determine the influence of light/dark cycling on microalgal biomass production and lipid accumulation. To achieve this goal, specific causative mechanisms were investigated using a metabolomics approach. Laboratory scale photoautotrophic cultivations of the oleaginous green microalga Chlamydomonas sp. JSC4 were performed under continuous light (LL) and light/dark (LD) conditions. RESULTS: Lipid accumulation and carbohydrate degradation were delayed under the LD condition compared with that under the LL condition. Metabolomic analysis revealed accumulation of phosphoenolpyruvate and decrease of glycerol 3-phosphate under the LD condition, suggesting that the imbalance of these metabolites is a source of delayed lipid accumulation. When accounting for light dosage, biomass yield under the LD condition was significantly higher than that under the LL condition. Dynamic metabolic profiling showed higher levels of lipid/carbohydrate anabolism (including production of 3-phosphoglycerate, fructose 6-phosphate, glucose 6-phosphate, phosphoenolpyruvate and acetyl-CoA) from CO2 under the LD condition, indicating higher CO2 fixation than that of the LL condition. CONCLUSIONS: Photoperiods define lipid accumulation and biomass production, and light/dark cycling was determined as a critical obstacle for lipid production in JSC4. Conversions of phosphoenolpyruvate to pyruvate and 3-phosphoglycerate to glycerol 3-phosphate are the candidate rate-limiting steps responsible for delayed lipid accumulation. The accumulation of substrates including ribulose 5-phosphate could be explained by the close relationship of increased biomass yield with enhanced CO2 fixation. The present study investigated the influence of light/dark cycling on lipid production by direct comparison with continuous illumination for the first time, and revealed underlying metabolic mechanisms and candidate metabolic rate-limiting steps during light/dark cycling. These findings suggest promising targets to metabolically engineer improved lipid production.

  Feb. 2019, Biotechnology for Biofuels, 12, 39 - 39, English, International magazine

  [Refereed]

  Scientific journal


• Disruption of poly (3-hydroxyalkanoate) depolymerase gene and overexpression of three poly (3-hydroxybutyrate) biosynthetic genes improve poly (3-hydroxybutyrate) production from nitrogen rich medium by Rhodobacter sphaeroides

  Akihiko Kondo

  Feb. 2019, Microbial Cell Factories, 18, 40, English

  [Refereed]

  Scientific journal


• 5-Hydroxymethylfurfural production from salt-induced photoautotrophically cultivated Chlorella sorokiniana

  Jerome Amoah, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Feb. 2019, Biochemical Engineering Journal, 142, 117 - 123, English

  [Refereed]

  Scientific journal


• Semiology of hyperkinetic seizures of frontal versus temporal lobe origin

  Akihiko Kondo

  2019, Epileptic Disorders

  Scientific journal


• Is additional mesial temporal resection necessary for intractable epilepsy with cavernous malformations in the temporal neocortex?

  Akihiko Kondo

  2019, Epilepsy & Behavior

  Scientific journal


• GH-10 and GH-11 Endo-1,4-β-xylanase enzymes from Kitasatospora sp. produce xylose and xylooligosaccharides from sugarcane bagasse with no xylose inhibition

  Nanik Rahmani, Prihardi Kahar, Puspita Lisdiyanti, Jaemin Lee, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

  A novel strategy for the low-cost, high-yield co-production of xylose and xylooligosaccharides together with no xylose inhibition was developed using a novel heterologous expression of XYN10Ks_480 endo-1,4-β-xylanase with a ricin-type β-trefoil type of domain and XYN11Ks_480 endo-1,4-β-xylanase with a CBM 2 superfamily from the Kitasatospora sp in an actinomycetes expression system. Xylose is the main building block for hemicellulose xylan. Our findings demonstrated high levels of expression and catalytic activity for XYN10Ks_480 during hydrolysis of the extracted xylan of bagasse, and three types of xylan-based substrates were used to produce xylose and xylooligosaccharides. However, hydrolysis by XYN11Ks_480 produced xylooligosaccharides without xylose formation. This study demonstrated how integrating sodium hypochlorite-extracted xylan and enzymatic hydrolysis could provide an alternative strategy for the generation of XOS from lignocellulosic material.

  Jan. 2019, Bioresource Technology, 272, 315 - 325, English, International magazine

  [Refereed]

  Scientific journal


• Less biomass and intracellular glutamate in anodic biofilms lead to efficient electricity generation by microbial fuel cells.

  Daisuke Sasaki, Kengo Sasaki, Yota Tsuge, Akihiko Kondo

  Background: Using a microbial fuel cell (MFC), we observed that a complex microbial community decomposed starch and transferred electrons to a graphite felt anode to generate current. In spite of the same reactor configuration, inoculum, substrate, temperature, and pH, MFCs produced different current and power density. To understand which factor(s) affected electricity generation, here, we analyzed a complex microbial community in an anodic biofilm and fermentation broth using Illumina MiSeq sequencing and metabolomics. Results: Microbial biomass on the anode was lower in MFCs generating more electricity (0.09-0.16 mg cm-2-anode) than in those generating less electricity (0.60-2.80 mg cm-2-anode), while being equal (3890-4196 mg L-1-broth) in the fermentation broth over the same operational period. Chemical oxygen demand removal and acetate concentration were also similar in fermentation broths. MFCs generating more electricity had relatively more exoelectrogenic bacteria, such as Geobacter sp., but fewer acetate-utilizing Methanosarcina sp. and/or Lactococcus sp. in anodic biofilms. Accordingly, anodic biofilms generating more electricity presented higher levels of most intracellular metabolites related to the tricarboxylic acid cycle and a higher intracellular ATP/ADP ratio, but a lower intracellular NADH/NAD+ ratio. Moreover, the level of intracellular glutamate, an essential metabolite for microbial anabolic reactions, correlated negatively with current density. Conclusion: Microbial growth on the anode and intracellular glutamate levels negatively affect electricity generation by MFCs. Reduced formation of anodic biofilm, in which intracellular glutamate concentration is 33.9 μmol g-cell-1 or less, favors the growth of acetate-utilizing Geobacter sp. on the anode and improves current generation.

  2019, Biotechnology for biofuels, 12, 72 - 72, English, International magazine

  [Refereed]

  Scientific journal


• Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure

  Daisuke Sasaki, Kengo Sasaki, Naoko Ikuta, Takahiro Yasuda, Itsuko Fukuda, Akihiko Kondo, Ro Osawa

  Nature Publishing Group, Dec. 2018, Scientific Reports, 8(1) (1), Article number: 435, English

  [Refereed]

  Scientific journal


• Muconic Acid Production Using Gene-Level Fusion Proteins in Escherichia coli

  Ryosuke Fujiwara, Shuhei Noda, Tsutomu Tanaka, Akihiko Kondo

  Nov. 2018, ACS Synthetic Biology, 7(11) (11), 2698 - 2705, English

  [Refereed]

  Scientific journal


• Improved permselectivity of forward osmosis membranes for efficient concentration of pretreated rice straw and bioethanol production

  Yihan Zhang, Keizo Nakagawa, Masafumi Shibuya, Kengo Sasaki, Tomoki Takahashi, Takuji Shintani, Tomohisa Yoshioka, Eiji Kamio, Akihiko Kondo, Hideto Matsuyama

  Nov. 2018, JOURNAL OF MEMBRANE SCIENCE, 566, 15 - 24, English

  [Refereed]

  Scientific journal


• Enhancing 3-hydroxypropionic acid production in combination with sugar supply engineering by cell surface-display and metabolic engineering of Schizosaccharomyces pombe

  TAKAYAMA Seiya, OZAKI Aiko, KONISHI Rie, OTOMO Chisako, KISHIDA Mayumi, HIRATA Yuuki, MATSUMOTO Takuya, TANAKA Tsutomu, KONDO Akihiko

  BACKGROUND: Economical production of value-added chemicals from renewable biomass is a promising path to sustainability. 3-Hydroxypropionic acid (3-HP) is an important chemical for building a bio-sustainable society. Establishment of 3-HP production from renewable resources such as glucose would provide a bio-sustainable alternative to the production of acrylic acid from fossil resources. RESULTS: Here, we describe metabolic engineering of the fission yeast Schizosaccharomyces pombe to enhance 3-HP production from glucose and cellobiose via the malonyl-CoA pathway. The mcr gene, encoding the malonyl-CoA reductase of Chloroflexus aurantiacus, was dissected into two functionally distinct fragments, and the activities of the encoded protein were balanced. To increase the cellular supply of malonyl-CoA and acetyl-CoA, we introduced genes encoding endogenous aldehyde dehydrogenase, acetyl-CoA synthase from Salmonella enterica, and endogenous pantothenate kinase. The resulting strain produced 3-HP at 1.0 g/L from a culture starting at a glucose concentration of 50 g/L. We also engineered the sugar supply by displaying beta-glucosidase (BGL) on the yeast cell surface. When grown on 50 g/L cellobiose, the beta-glucosidase-displaying strain consumed cellobiose efficiently and produced 3-HP at 3.5 g/L. Under fed-batch conditions starting from cellobiose, this strain produced 3-HP at up to 11.4 g/L, corresponding to a yield of 11.2% (g-3-HP/g-glucose; given that 1 g cellobiose corresponds to 1.1 g glucose upon digestion). CONCLUSIONS: In this study, we constructed a series of S. pombe strains that produced 3-HP via the malonyl-CoA pathway. Our study also demonstrated that BGL display using cellobiose and/or cello-oligosaccharides as a carbon source has the potential to improve the titer and yield of malonyl-CoA- and acetyl-CoA-derived compounds.

  Nov. 2018, Microbial Cell Factories, 17(1) (1), 176 - 176, English, International magazine

  [Refereed]

  Scientific journal


• Deletion of DNA ligase IV homolog confers higher gene targeting efficiency on homologous recombination in Komagataella phaffii

  Yoichiro Ito, Toru Watanabe, Shimpei Aikawa, Teruyuki Nishi, Tozo Nishiyama, Yasuyuki Nakamura, Tomohisa Hasunuma, Yuji Okubo, Jun Ishii, Akihiko Kondo

  Oxford University Press (OUP), Nov. 2018, FEMS Yeast Research, 18(7) (7), foy074, English

  [Refereed]

  Scientific journal


• De novo design of biosynthetic pathways for bacterial production of bulk chemicals and biofuels

  Akihiko Kondo

  Oct. 2018, FEMS Microbiology Letters, 365(20) (20), fny215, English

  [Refereed]

  Scientific journal


• Repeated ethanol fermentation from membrane-concentrated sweet sorghum juice using the flocculating yeast Saccharomyces cerevisiae F118 strain

  WIJAYA Hans, SASAKI Kengo, KAHAR Prihardi, YOPI, KAWAGUCHI Hideo, SAZUKA Takashi, OGINO Chiaki, PRASETYA Bambang, KONDO Akihiko

  The aim of this study was to construct a cost-effective method for repeated bioethanol production using membrane (ultrafiltration permeation and nanofiltration concentration)-concentrated sweet sorghum juice by using flocculent Saccharomyces cerevisiae F118 strain. With low initial dry cell concentrations at around 0.28-0.35 g L-1, the S. cerevisiae F118 strain provided an ethanol titer of 86.19 ± 1.15 g L-1 (theoretical ethanol yield of 70.77%), which was higher than the non-flocculent S. cerevisiae BY4741 strain at 33.92 ± 0.99 g L-1 after 24 h fermentation. This result was correlated with higher gene expressions of the sucrose-hydrolysing enzyme invertase, sugar phosphorylation, and pyruvate-to-ethanol pathways in the F118 strain compared with the BY4741 strain. Sequential fed-batch fermentation was conducted, and the F118 strain was easily separated from the fermentation broth via the formation of flocs and sediment. After the 5th cycle of fermentation with the F118 strain, the ethanol concentration reached 100.37 g L-1.

  Oct. 2018, Bioresource Technology, 265, 542 - 547, English, International magazine

  [Refereed]

  Scientific journal


• Development of cell recycle technology incorporating nutrient supplementation for lignocellulosic ethanol fermentation using industrial yeast Saccharomyces cerevisiae

  Shinji Hama, Maki Kihara, Hideo Noda, Akihiko Kondo

  Elsevier B.V., Sep. 2018, Biochemical Engineering Journal, 137, 23 - 29, English

  [Refereed]

  Scientific journal


• Selection of oleaginous yeasts capable of high lipid accumulation during challenges from inhibitory chemical compounds

  Ario Betha Juanssilfero, Prihardi Kahar, Rezky Lastinov Amza, Nao Miyamoto, Hiromi Otsuka, Hana Matsumoto, Chie Kihira, Ahmad Thontowi, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  Elsevier B.V., Sep. 2018, Biochemical Engineering Journal, 137, 182 - 191, English

  [Refereed]

  Scientific journal


• Widespread effect of N-acetyl-D-glucosamine assimilation on the metabolisms of amino acids, purines, and pyrimidines in Scheffersomyces stipitis

  Akihiko Kondo

  Sep. 2018, Microbial Cell Factories, 17, 153, English

  [Refereed]

  Scientific journal


• Targeted Nucleotide Editing Technologies for Microbial Metabolic Engineering

  Akihiko Kondo

  Sep. 2018, Biotechnology Journal, 13(9) (9), 1700596, English

  [Refereed]

  Scientific journal


• Development of a strictly regulated xylose-induced expression system in Streptomyces

  Akihiko Kondo

  Sep. 2018, Microbial Cell Factories, 17, 151, English

  [Refereed]

  Scientific journal


• 油脂酵母Lipomyces starkeyiD35株の繰り返し発酵による脂肪酸組成変化解析

  宮本 捺央, Kahar Prihardi, 荻野 千秋, 近藤 昭彦

  (公社)日本生物工学会, Aug. 2018, 日本生物工学会大会講演要旨集, 平成30年度, 113 - 113, Japanese


• Herbicide tolerance-assisted multiplex targeted nucleotide substitution in rice

  Akihiko Kondo

  Aug. 2018, Data in Brief, 20, 1325 - 1331, English

  [Refereed]

  Scientific journal


• Engineering Human Epidermal Growth Receptor 2-Targeting Hepatitis B Virus Core Nanoparticles for siRNA Delivery in Vitro and in Vivo

  Izzat F. M. Suffian, Julie T.-W. Wang, Farid N. Faruqu, Julio Benitez, Yuya Nishimura, Chiaki Ogino, Akihiko Kondo, Khuloud T. Al-Jamal

  Jul. 2018, ACS Applied Nano Materials, 1(7) (7), 3269 - 3282, English

  Scientific journal


• 分裂酵母を用いた有機酸生産技術の開発

  TANAKA TSUTOMU, KONDO AKIHIKO

  化学工業社, Jul. 2018, ケミカルエンジニヤリング, 特集=革新技術が拓く新しい可能性, 63(7) (7), 7 - 10, Japanese

  Scientific journal


• Temperature enhanced succinate production concurrent with increased central metabolism turnover in the cyanobacterium Synechocystis sp. PCC 6803

  Tomohisa Hasunuma, Mami Matsuda, Yuichi Kato, Christopher John Vavricka, Akihiko Kondo

  Academic Press Inc., Jul. 2018, Metabolic Engineering, 48, 109 - 120, English

  [Refereed]

  Scientific journal


• Metabolic engineering of Corynebacterium glutamicum for production of sunscreen shinorine.

  Yota Tsuge, Hideo Kawaguchi, Shogo Yamamoto, Yoshiko Nishigami, Masahiro Sota, Chiaki Ogino, Akihiko Kondo

  Ultraviolet-absorbing chemicals are useful in cosmetics and skin care to prevent UV-induced skin damage. We demonstrate here that heterologous production of shinorine, which shows broad absorption maxima in the UV-A and UV-B region. A shinorine producing Corynebacterium glutamicum strain was constructed by expressing four genes from Actinosynnema mirum DSM 43827, which are responsible for the biosynthesis of shinorine from sedoheptulose-7-phosphate in the pentose phosphate pathway. Deletion of transaldolase encoding gene improved shinorine production by 5.2-fold. Among the other genes in pentose phosphate pathway, overexpression of 6-phosphogluconate dehydrogenase encoding gene further increased shinorine production by 60% (19.1 mg/L). The genetic engineering of the pentose phosphate pathway in C. glutamicum improved shinorine production by 8.3-fold in total, and could be applied to produce the other chemicals derived from sedoheptulose-7-phosphate.

  Jul. 2018, Bioscience, biotechnology, and biochemistry, 82(7) (7), 1252 - 1259, English, International magazine

  [Refereed]

  Scientific journal


• In vivo tissue distribution and safety of polyacrylic acid-modified titanium peroxide nanoparticles as novel radiosensitizers.

  Kenta Morita, Takahiro Suzuki, Yuya Nishimura, Kazuhisa Matsumoto, Chiya Numako, Kazuyoshi Sato, Masao Nakayama, Ryohei Sasaki, Chiaki Ogino, Akihiko Kondo

  Polyacrylic acid (PAA)-modified titanium peroxide nanoparticles (PAA-TiOx NPs) are promising radiosensitizers. PAA-TiOx NPs were synthesized from commercial TiO2 nanoparticles that were modified with PAA and functionalized by H2O2 treatment. To realize practical clinical uses for PAA-TiOx NPs, their tissue distribution and acute toxicity were evaluated using healthy mice and mice bearing tumors derived from xenografted MIAPaCa-2 human pancreatic cancer cells. Healthy mice were injected with PAA-TiOx NPs at 25 mg/kg body weight via the tail vein, and tumor-bearing mice were injected either into the tumor locally or via the tail vein. The concentration of PAA-TiOx NPs in major organs was determined over time using inductively coupled-plasma atomic emission spectrometry. After 1 h, 12% of the PAA-TiOx NP dose had accumulated in the tumor, and 2.8% of the dose remained after 1 week. Such high accumulation could be associated with enhanced permeability and retention effects of the tumor, as PAA-TiOx NPs are composed of inorganic particles and polymers, without tumor-targeting molecules. The liver accumulated the largest proportion of the injected nanoparticles, up to 42% in tumor-bearing mice. Blood biochemical parameters were also investigated after intravenous injection of PAA-TiOx NPs in healthy mice. PAA-TiOx NPs invoked a slight change in various liver-related biochemical parameters, but no liver injury was observed over the practical dose range. In the future, PAA-TiOx NPs should be modified to prevent accumulation in the liver and minimize risk to patients.

  Jul. 2018, Journal of bioscience and bioengineering, 126(1) (1), 119 - 125, English, Domestic magazine

  [Refereed]

  Scientific journal


• A stable, autonomously replicating plasmid vector containing Pichia pastoris centromeric DNA

  Yasuyuki Nakamura, Teruyuki Nishi, Risa Noguchi, Yoichiro Ito, Toru Watanabe, Tozo Nishiyama, Sinpei Aikawa, Tomohisa Hasunuma, Jun Ishii, Yuji Okubo, Akihiko Kondo

  The methylotrophic yeast Pichia pastoris is widely used to produce recombinant proteins, taking advantage of this species' high-density cell growth and strong ability to secrete proteins. Circular plasmids containing the P. pastoris-specific autonomously replicating sequence (PARS1) permit transformation of P. pastoris with higher efficiency than obtained following chromosomal integration by linearized DNA. Unfortunately, however, existing autonomously replicating plasmids are known to be inherently unstable. In this study, we used transcriptome sequencing (RNA-seq) data and genome sequence information to independently identify, on each of the four chromosomes, centromeric DNA sequences consisting of long inverted repeat sequences. By examining the chromosome 2 centromeric DNA sequence (Cen2) in detail, we demonstrate that an ∼111-bp region located at one end of the putative centromeric sequence had autonomous replication activity. In addition, the full-length Cen2 sequence, which contains two long inverted repeat sequences and a nonrepetitive central core region, is needed for the accurate replication and distribution of plasmids in P. pastoris. Thus, we constructed a new, stable, autonomously replicating plasmid vector that harbors the entire Cen2 sequence; this episome facilitates genetic manipulation in P. pastoris, providing high transformation efficiency and plasmid stability. IMPORTANCE Secretory production of recombinant proteins is the most important application of the methylotrophic yeast Pichia pastoris, a species that permits mass production of heterologous proteins. To date, the genetic engineering of P. pastoris has relied largely on integrative vectors due to the lack of user-friendly tools. Autonomously replicating Pichia plasmids are expected to facilitate genetic manipulation; however, the existing systems, which use autonomously replicating sequences (ARSs) such as the P. pastoris-specific ARS (PARS1), are known to be inherently unstable for plasmid replication and distribution. Recently, the centromeric DNA sequences of P. pastoris were identified in back-to-back studies published by several groups; therefore, a new episomal plasmid vector with centromere DNA as a tool for genetic manipulation of P. pastoris is ready to be developed.

  American Society for Microbiology, Jul. 2018, Applied and Environmental Microbiology, 84(15) (15), e02882 - 17, English

  [Refereed]

  Scientific journal


• Effect of inoculum size on single-cell oil production from glucose and xylose using oleaginous yeast Lipomyces starkeyi

  Ario B. Juanssilfero, Prihardi Kahar, Rezky L. Amza, Nao Miyamoto, Hiromi Otsuka, Hana Matsumoto, Chie Kihira, Ahmad Thontowi, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  Elsevier B.V., Jun. 2018, Journal of Bioscience and Bioengineering, 125(6) (6), 695 - 702, English

  [Refereed]

  Scientific journal


• A pyruvate carbon flux tugging strategy for increasing 2,3-butanediol production and reducing ethanol subgeneration in the yeast Saccharomyces cerevisiae

  Akihiko Kondo

  Jun. 2018, Biotechnology for Biofuels, (11) (11), 180, English

  [Refereed]

  Scientific journal


• A Procedure for Precise Determination of Glutathione Produced by Saccharomyces cerevisiae

  Akihiko Kondo

  Jun. 2018, Bio-Protocol, 8(12) (12), e2887, English

  [Refereed]

  Scientific journal


• Metabolome analysis-based design and engineering of a metabolic pathway in Corynebacterium glutamicum to match rates of simultaneous utilization of d-glucose and l-arabinose

  Hideo Kawaguchi, Kumiko Yoshihara, Kiyotaka Y. Hara, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  BioMed Central Ltd., May 2018, Microbial Cell Factories, 17(1) (1), 76, English

  [Refereed]

  Scientific journal


• Efficient 3-Hydroxybutyrate Production by Quiescent Escherichia coli Microbial Cell Factories is Facilitated by Indole-Induced Proteomic and Metabolomic Changes

  Nicholas M. Thomson, Tomokazu Shirai, Marco Chiapello, Akihiko Kondo, Krishna J. Mukherjee, Easan Sivaniah, Keiji Numata, David K. Summers

  Wiley-VCH Verlag, May 2018, Biotechnology Journal, 13(5) (5), 1700571, English

  [Refereed]

  Scientific journal


• Electrical-biological hybrid system for CO2 reduction

  Yohei Tashiro, Shinichi Hirano, Morgan M. Matson, Shota Atsumi, Akihiko Kondo

  Academic Press Inc., May 2018, Metabolic Engineering, 47, 211 - 218, English

  [Refereed]

  Scientific journal


• Metabolic Engineering of Lactobacillus plantarum for Direct l-Lactic Acid Production From Raw Corn Starch

  Kenji Okano, Gentaro Uematsu, Shinji Hama, Tsutomu Tanaka, Hideo Noda, Akihiko Kondo, Kohsuke Honda

  Wiley-VCH Verlag, May 2018, Biotechnology Journal, 13(5) (5), 1700517, English

  [Refereed]

  Scientific journal


• How lipase technology contributes to evolution of biodiesel production using multiple feedstocks

  Shinji Hama, Hideo Noda, Akihiko Kondo

  Elsevier Ltd, Apr. 2018, Current Opinion in Biotechnology, 50, 57 - 64, English

  [Refereed]

  Scientific journal


• Editorial overview: Energy biotechnology: Biotechnology solutions for our energy needs

  Hal S Alper, Akihiko Kondo

  Elsevier Ltd, Apr. 2018, Current Opinion in Biotechnology, 50, v - vi, English

  [Refereed]

  Scientific journal


• Modifying Expression Modes of Human Neurotensin Receptor Type 1 Alters Sensing Capabilities for Agonists in Yeast Signaling Biosensor

  Hiroki Hashi, Yasuyuki Nakamura, Jun Ishii, Akihiko Kondo

  Wiley-VCH Verlag, Apr. 2018, Biotechnology Journal, 13(4) (4), e1700522, English

  [Refereed]

  Scientific journal


• Genetic and physiological basis for antibody production by Kluyveromyces marxianus

  Akihiko Kondo

  Apr. 2018, AMB Express, 8, 56, English

  [Refereed]

  Scientific journal


• Electrochemical biotechnologies minimizing the required electrode assemblies.

  Kengo Sasaki, Daisuke Sasaki, Kazuhide Kamiya, Shuji Nakanishi, Akihiko Kondo, Souichiro Kato

  Microbial electrochemical systems (MESs) are expected to be put into practical use as an environmental technology that can support a future environmentally friendly society. However, conventional MESs present a challenge of inevitably increasing initial investment, mainly due to requirements for a large numbers of electrode assemblies. In this review, we introduce electrochemical biotechnologies that are under development and can minimize the required electrode assemblies. The novel biotechnologies, called electro-fermentation and indirect electro-stimulation, can drive specific microbial metabolism by electrochemically controlling intercellular and extracellular redox states, respectively. Other technologies, namely electric syntrophy and microbial photo-electrosynthesis, obviate the need for electrode assemblies, instead stimulating targeted reactions by using conductive particles to create new metabolic electron flows.

  Apr. 2018, Current opinion in biotechnology, 50, 182 - 188, English, International magazine

  [Refereed]

  Scientific journal


• Deaminase-mediated multiplex genome editing in Escherichia coli

  Satomi Banno, Keiji Nishida, Takayuki Arazoe, Hitoshi Mitsunobu, Akihiko Kondo

  Nature Publishing Group, Apr. 2018, Nature Microbiology, 3(4) (4), 423 - 429, English

  [Refereed]

  Scientific journal


• Metabolic engineering of the 2-ketobutyrate biosynthetic pathway for 1-propanol production in Saccharomyces cerevisiae

  Yuya Nishimura, Terumi Matsui, Jun Ishii, Akihiko Kondo

  BioMed Central Ltd., Mar. 2018, Microbial Cell Factories, 17(1) (1), 38, English

  [Refereed]

  Scientific journal


• Enhanced cell-surface display of a heterologous protein using SED1 anchoring system in SED1-disrupted Saccharomyces cerevisiae strain

  Takahiro Bamba, Kentaro Inokuma, Tomohisa Hasunuma, Akihiko Kondo

  Elsevier B.V., Mar. 2018, Journal of Bioscience and Bioengineering, 125(3) (3), 346 - 352, English

  [Refereed]

  Scientific journal


• Effective usage of sorghum bagasse: Optimization of organosolv pretreatment using 25% 1-butanol and subsequent nanofiltration membrane separation

  Hiroshi Teramura, Kengo Sasaki, Tomoko Oshima, Hideo Kawaguchi, Chiaki Ogino, Takashi Sazuka, Akihiko Kondo

  Mar. 2018, Bioresource Technology, 252, 157 - 164, English

  [Refereed]

  Scientific journal


• Direct and highly productive conversion of cyanobacteria Arthrospira platensis to ethanol with CaCl2 addition

  Shimpei Aikawa, Kentaro Inokuma, Satoshi Wakai, Kengo Sasaki, Chiaki Ogino, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo

  BioMed Central Ltd., Feb. 2018, Biotechnology for Biofuels, 11(1) (1), 50, English

  [Refereed]

  Scientific journal


• Optimization of cellulolytic enzyme components through engineering Trichoderma reesei and on-site fermentation using the soluble inducer for cellulosic ethanol production from corn stover

  Yong-Hao Li, Xiao-Yue Zhang, Fei Zhang, Liang-Cai Peng, Da-Bing Zhang, Akihiko Kondo, Feng-Wu Bai, Xin-Qing Zhao

  BioMed Central Ltd., Feb. 2018, Biotechnology for Biofuels, 11(1) (1), 49, English

  [Refereed]

  Scientific journal


• Surgical Resection of Amygdala and Uncus

  Akihiko Kondo

  2018, Neurologia Medico-Chirurgica

  Scientific journal


• Working memory deficit in drug-resistant epilepsy with an amygdala lesion

  Akihiko Kondo

  2018, Epilepsy & Behavior Case Reports

  Scientific journal


• Successful detection of dysbiosis and altered short-chain-fatty acids levels in in vitro colonic microbiota culture system using faecal samples of ulcerative colitis

  Akihiko Kondo

  2018, Journal of Crohn's and Colitis

  Scientific journal


• Microbial platform to synthesize aromatic chemicals and the derivatives

  Akihiko Kondo

  2018, FEBS Open Bio

  Scientific journal


• Portal Venous Gas Due to Decompression Sickness

  Akihiko Kondo

  2018, Internal Medicine

  Scientific journal


• Metabolic design of Escherichia coli for production of shikimate pathway derivatives

  Akihiko Kondo

  2018, Basic & Clinical Pharmacology & Toxicology

  Scientific journal


• Impact of Automated External Defibrillator as a Recent Innovation for the Resuscitation of Cardiac Arrest Patients in an Urban City of Japan

  Akihiko Kondo

  2018, Journal of Emergencies, Trauma and Shock

  Scientific journal


• Metabolic engineering of S. pombe via CRISPR-CAS9 genome editing for 3-hydroxypropionic acid production from glucose and cellobiose

  Akihiko Kondo

  2018, Basic & Clinical Pharmacology & Toxicology

  Scientific journal


• Massive intraperitoneal free air induced by pneumothorax and pneumomediastinum

  Akihiko Kondo

  2018, International Journal of Surgery Case Reports

  Scientific journal


• Metabolic design of Escherichia coli for muconic acid production

  Akihiko Kondo

  2018, FEBS Open Bio

  Scientific journal


• Characteristics of oxygenated hemoglobin concentration change during pleasant and unpleasant image-recall tasks in patients with depression: Comparison with healthy subjects

  Akihiko Kondo

  2018, Psychiatry and Clinical Neurosciences

  Scientific journal


• Comparison of changes in oxygenated hemoglobin during the tree-drawing task between patients with schizophrenia and healthy controls

  Akihiko Kondo

  2018, Neuropsychiatric Disease and Treatment

  Scientific journal


• A Case of Near-Fatal Drowning Caused by an Attack from a Wild Boar

  Akihiko Kondo

  2018, Wilderness and Environmental Medicine

  Scientific journal


• A classification system for verifying the long-term efficacy of resective surgery for drug-resistant seizures

  Akihiko Kondo

  2018, Epilepsy Research

  Scientific journal


• Analyzing Neural Activity and Connectivity Using Intracranial EEG Data with SPM Software

  Akihiko Kondo

  2018, Journal of Visualized Experiments

  Scientific journal


• P-45 Caffeic acid production by simultaneous saccharification and fermentation of kraft pulp using recombinant Escherichia coli

  Kawaguchi Hideo, Katsuyama Yohei, Du Danyao, Nakamura-Tsuruta Sachiko, Minami Hiromichi, Ogino Chiaki, Ohnishi Yasuo, Kondo Akihiko

  Caffeic acid (3,4-dihydroxycinnamic acid) serves as a building block for thermoplastics and was recently produced from glucose by microbial fermentation. To produce caffeic acid from inedible cellulose, separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) reactions were compared using kraft pulp as lignocellulosic feedstock. Here, a tyrosine-overproducing Escherichia coli strain was metabolically engineered to produce caffeic acid from glucose by expressing the genes encoding a tyrosine ammonia lyase (fevV) from Streptomyces sp. WK-5344 and 4-hydroxyphenyllactate 3-hydroxylase (hpaBC) from Pseudomonas aeruginosa. Using the resulting recombinant strain, the maximum yield of caffeic acid in SSF (233 mg/L) far exceeded that by SHF (37.9 mg/L). In the SSF with low cellulase loads (≤2.5 filter paper unit/g glucan), caffeic acid production was markedly increased, while almost no glucose accumulation was detected, indicating that the E. coli cells experienced glucose limitation in this culture condition. Caffeic acid yield was also negatively correlated with the glucose concentration in the fermentation medium. These observations suggest that glucose limitation in SSF is crucial for improving caffeic acid yield, owing to reduced by-product formation and fermentation inhibitor accumulation.

  The Japan Institute of Energy, 2018, Proceedings of the Conference on Biomass Science, 13(0) (0), 135 - 136, Japanese

  [Refereed]


• Sortase A-Assisted Metabolic Enzyme Ligation in Escherichia coli for Enhancing Metabolic Flux.

  Matsumoto T, Tanaka T, Kondo A

  Metabolic engineering has been an important approach for microbial bio-production. To produce bio-chemicals with engineered microorganisms, metabolic pathways have been edited using several common strategies, including gene disruption, gene overexpression, and gene attenuation. Here, we demonstrated metabolic channeling based on enzymatic metabolic enzyme ligation as a noteworthy approach for enhancing a desired metabolic flux. To achieve metabolic channeling , the metabolic enzymes should be in close proximity in cells. In the literature, several methodologies have been recently applied to achieve metabolic channeling . Meanwhile, we have proposed a strategy for possessing metabolic enzymes in close proximity, by utilizing sortase A as a stapler to tether such enzymes in Escherichia coli. By tethering metabolic enzymes that catalyze the reactions before and after a target metabolite, the metabolic flux may be enhanced. This chapter describes the approach for enhancing acetate-producing flux by sortase-A-assisted metabolic ligation in E. coli.

  2018, Methods in molecular biology (Clifton, N.J.), 1772, 125 - 136, English, International magazine

  [Refereed]

  Scientific journal


• Streptavidin-hydrogel prepared by sortase A-assisted click chemistry for enzyme immobilization on an electrode

  Takuya Matsumoto, Yuki Isogawa, Tsutomu Tanaka, Akihiko Kondo

  Jan. 2018, BIOSENSORS & BIOELECTRONICS, 99, 56 - 61, English

  [Refereed]

  Scientific journal


• Selection of yeast Saccharomyces cerevisiae promoters available for xylose cultivation and fermentation

  Yumiko Nambu-Nishida, Yuri Sakihama, Jun Ishii, Tomohisa Hasunuma, Akihiko Kondo

  Elsevier B.V., Jan. 2018, Journal of Bioscience and Bioengineering, 125(1) (1), 76 - 86, English

  [Refereed]

  Scientific journal


• Xylanase and feruloyl esterase from actinomycetes cultures could enhance sugarcane bagasse hydrolysis in the production of fermentable sugars

  Nanik Rahmani, Prihardi Kahar, Puspita Lisdiyanti, Euis Hermiati, Jaemin Lee, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

  Japan Society for Bioscience Biotechnology and Agrochemistry, 2018, Bioscience, Biotechnology and Biochemistry, 82(5) (5), 904 - 915, English

  [Refereed]

  Scientific journal


• Changes in the microbial consortium during dark hydrogen fermentation in a bioelectrochemical system increases methane production during a two-stage process.

  Kengo Sasaki, Daisuke Sasaki, Yota Tsuge, Masahiko Morita, Akihiko Kondo

  Background: Bioelectrochemical systems (BESs) are an innovative technology developed to influence conventional anaerobic digestion. We examined the feasibility of applying a BES to dark hydrogen fermentation and its effects on a two-stage fermentation process comprising hydrogen and methane production. The BES used low-cost, low-reactivity carbon sheets as the cathode and anode, and the cathodic potential was controlled at - 1.0 V (vs. Ag/AgCl) with a potentiostat. The operation used 10 g/L glucose as the major carbon source. Results: The electric current density was low throughout (0.30-0.88 A/m2 per electrode corresponding to 0.5-1.5 mM/day of hydrogen production) and water electrolysis was prevented. At a hydraulic retention time of 2 days with a substrate pH of 6.5, the BES decreased gas production (hydrogen and carbon dioxide contents: 52.1 and 47.1%, respectively), compared to the non-bioelectrochemical system (NBES), although they had similar gas compositions. In addition, a methane fermenter (MF) was applied after the BES, which increased gas production (methane and carbon dioxide contents: 85.1 and 14.9%, respectively) compared to the case when the MF was applied after the NBES. Meta 16S rRNA sequencing revealed that the BES accelerated the growth of Ruminococcus sp. and Veillonellaceae sp. and decreased Clostridium sp. and Thermoanaerobacterium sp., resulting in increased propionate and ethanol generation and decreased butyrate generation; however, unknowingly, acetate generation was increased in the BES. Conclusions: The altered redox potential in the BES likely transformed the structure of the microbial consortium and metabolic pattern to increase methane production and decrease carbon dioxide production in the two-stage process. This study showed the utility of the BES to act on the microbial consortium, resulting in improved gas production from carbohydrate compounds.

  2018, Biotechnology for biofuels, 11, 173 - 173, English, International magazine

  [Refereed]

  Scientific journal


• Genotypic effects on sugar and by-products of liquid hydrolysates and on saccharification of acidinsoluble residues from wheat straw

  Ryoko Ohno, Hiroshi Teramura, Chiaki Ogino, Akihiko Kondo, Shigeo Takumi

  2018, Genes and Genetic Systems, 93(1) (1), 1 - 7, English

  [Refereed]

  Scientific journal


• Inheritance of co-edited genes by CRISPR-based targeted nucleotide substitutions in rice

  Zenpei Shimatani, Ushio Fujikura, Hisaki Ishii, Yusuke Matsui, Minoru Suzuki, Yuki Ueke, Ken-ichiro Taoka, Rie Terada, Keiji Nishida, Akihiko Kondo

  Elsevier Masson SAS, 2018, Plant Physiology and Biochemistry, 131, 78 - 83, English

  [Refereed]

  Scientific journal


• Biotransformation of ferulic acid to protocatechuic acid by Corynebacterium glutamicum ATCC 21420 engineered to express vanillate O-demethylase.

  Naoko Okai, Takaya Masuda, Yasunobu Takeshima, Kosei Tanaka, Ken-Ichi Yoshida, Masanori Miyamoto, Chiaki Ogino, Akihiko Kondo

  Ferulic acid (4-hydroxy-3-methoxycinnamic acid, FA) is a lignin-derived phenolic compound abundant in plant biomass. The utilization of FA and its conversion to valuable compounds is desired. Protocatechuic acid (3,4-dihydroxybenzoic acid, PCA) is a precursor of polymers and plastics and a constituent of food. A microbial conversion system to produce PCA from FA was developed in this study using a PCA-producing strain of Corynebacterium glutamicum F (ATCC 21420). C. glutamicum strain F grown at 30 °C for 48 h utilized 2 mM each of FA and vanillic acid (4-hydroxy-3-methoxybenzoic acid, VA) to produce PCA, which was secreted into the medium. FA may be catabolized by C. glutamicum through proposed (I) non-β-oxidative, CoA-dependent or (II) β-oxidative, CoA-dependent phenylpropanoid pathways. The conversion of VA to PCA is the last step in each pathway. Therefore, the vanillate O-demethylase gene (vanAB) from Corynebacterium efficiens NBRC 100395 was expressed in C. glutamicum F (designated strain FVan) cultured at 30 °C in AF medium containing FA. Strain C. glutamicum FVan converted 4.57 ± 0.07 mM of FA into 2.87 ± 0.01 mM PCA after 48 h with yields of 62.8% (mol/mol), and 6.91 mM (1064 mg/L) of PCA was produced from 16.0 mM of FA after 12 h of fed-batch biotransformation. Genomic analysis of C. glutamicum ATCC 21420 revealed that the PCA-utilization genes (pca cluster) were conserved in strain ATCC 21420 and that mutations were present in the PCA importer gene pcaK.

  Dec. 2017, AMB Express, 7(1) (1), 130 - 130, English, International magazine

  [Refereed]

  Scientific journal


• Improved ethanol production at high temperature by consolidated bioprocessing using Saccharomyces cerevisiae strain engineered with artificial zinc finger protein

  M. Mahfuza Khatun, Xinshui Yu, Akihiko Kondo, Fengwu Bai, Xinqing Zhao

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245(B) (B), 1447 - 1454, English

  [Refereed]

  Scientific journal


• Current advances on fermentative biobutanol production using third generation feedstock

  Yue Wang, Shih-Hsin Ho, Hong-Wei Yen, Dillirani Nagarajan, Nan-Qi Ren, Shuangfei Li, Zhangli Hu, Duu-Jong Lee, Akihiko Kondo, Jo-Shu Chang

  Elsevier Inc., Dec. 2017, Biotechnology Advances, 35(8) (8), 1049 - 1059, English

  [Refereed]

  Scientific journal


• Development of fed-batch process for high-yielding beta-glucosidase displayed on cell surface of industrial yeast Saccharomyces cerevisiae

  Kaoru Onodera, Shinji Hama, Ayumi Yoshida, Hideo Noda, Akihiko Kondo

  Dec. 2017, BIOCHEMICAL ENGINEERING JOURNAL, 128, 195 - 200, English

  [Refereed]

  Scientific journal


• Production of chemicals and proteins using biomass-derived substrates from a Streptomyces host

  Norimasa Kashiwagi, Chiaki Ogino, Akihiko Kondo

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245, 1655 - 1663, English

  [Refereed]

  Scientific journal


• Microbial conversion of biomass into bio-based polymers

  Hideo Kawaguchi, Chiaki Ogino, Akihiko Kondo

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245(B) (B), 1664 - 1673, English

  [Refereed]

  Scientific journal


• Mannan endo-1,4-β-mannosidase from Kitasatospora sp. isolated in Indonesia and its potential for production of mannooligosaccharides from mannan polymers

  Nanik Rahmani, Norimasa Kashiwagi, Jae Min Lee, Satoko Niimi-Nakamura, Hana Matsumoto, Prihardi Kahar, Puspita Lisdiyanti, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

  Springer Verlag, Dec. 2017, AMB Express, 7(1) (1), 100, English

  [Refereed]

  Scientific journal


• Future insights in fungal metabolic engineering

  Satoshi Wakai, Takayoshi Arazoe, Chiaki Ogino, Akihiko Kondo

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245(B) (B), 1314 - 1326, English

  [Refereed]

  Scientific journal


• Development and evaluation of consolidated bioprocessing yeast for ethanol production from ionic liquid-pretreated bagasse

  Jerome Amoah, Naoya Ishizue, Miki Ishizaki, Misa Yasuda, Kenji Takahashi, Kazuaki Ninomiya, Ryosuke Yamada, Akihiko Kondo, Chiaki Ogino

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245(B) (B), 1413 - 1420, English

  [Refereed]

  Scientific journal


• Challenges of non-flocculating Saccharomyces cerevisiae haploid strain against inhibitory chemical complex for ethanol production

  Prihardi Kahar, Eny Ida Riyanti, Hiromi Otsuka, Hana Matsumoto, Chie Kihira, Chiaki Ogino, Akihiko Kondo

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245(B) (B), 1436 - 1446, English

  [Refereed]

  Scientific journal


• Improvement of Xylose Fermentation Ability under Heat and Acid Co-Stress in Saccharomyces cerevisiae Using Genome Shuffling Technique

  Akihiko Kondo

  Dec. 2017, Frontiers in Bioengineering and Biotechnology, 5, 81, English

  [Refereed]

  Scientific journal


• Glutathione production from mannan-based bioresource by mannanase/mannosidase expressing Saccharomyces cerevisiae

  Alex Prima, Kiyotaka Y. Hara, Apridah Cameliawati Djohan, Norimasa Kashiwagi, Prihardi Kahar, Jun Ishii, Hideki Nakayama, Fumiyoshi Okazaki, Bambang Prasetya, Akihiko Kondo, Yopi, Chiaki Ogino

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245(B) (B), 1400 - 1406, English

  [Refereed]

  Scientific journal


• Evolutionary engineering of salt-resistant Chlamydomonas sp strains reveals salinity stress-activated starch-to-lipid biosynthesis switching

  Yuichi Kato, Shih-Hsin Ho, Christopher J. Vavricka, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245(part B) (part B), 1484 - 1490, English

  [Refereed]

  Scientific journal


• Engineering metabolic pathways in Escherichia coli for constructing a "microbial chassis" for biochemical production

  Akihiko Kondo

  Dec. 2017, Bioresource Technology, 245(B) (B), 1362 - 1368, English

  [Refereed]

  Scientific journal


• Conversion of Chlamydomonas sp JSC4 lipids to biodiesel using Fusarium heterosporum lipase-expressing Aspergillus oryzae whole-cell as biocatalyst

  Jerome Amoah, Shih-Hsin Ho, Shinji Hama, Ayumi Yoshida, Akihito Nakanishi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Dec. 2017, ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 28, 16 - 23, English

  [Refereed]

  Scientific journal


• 1,5-Diaminopentane production from xylooligosaccharides using metabolically engineered Corynebacterium glutamicum displaying beta-xylosidase on the cell surface

  Kenta Imao, Rie Konishi, Mayumi Kishida, Yuuki Hirata, Shota Segawa, Noriko Adachi, Rena Matsuura, Yota Tsuge, Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245(B) (B), 1684 - 1691, English

  [Refereed]

  Scientific journal


• Screening and evaluation of aptamers against somatostatin, and sandwich-like monitoring of somatostatin based on atomic force microscopy

  Musashi Takenaka, Tomokazu Amino, Yusuke Miyachi, Chiaki Ogino, Akihiko Kondo

  Nov. 2017, SENSORS AND ACTUATORS B-CHEMICAL, 252, 813 - 821, English

  [Refereed]

  Scientific journal


• Bear-trap sensing of somatostatin via split aptamers and atomic force microscopy

  Musashi Takenaka, Tomokazu Amino, Yusuke Miyachi, Chiaki Ogino, Akihiko Kondo

  Nov. 2017, SENSORS AND ACTUATORS B-CHEMICAL, 252, 600 - 605, English

  [Refereed]

  Scientific journal


• Transporter engineering in biomass utilization by yeast

  Kiyotaka Y. Hara, Jyumpei Kobayashi, Ryosuke Yamada, Daisuke Sasaki, Yuki Kuriya, Yoko Hirono-Hara, Jun Ishii, Michihiro Araki, Akihiko Kondo

  Nov. 2017, FEMS YEAST RESEARCH, 17(7) (7), fox061, English

  [Refereed]

  Scientific journal


• Engineering a synthetic pathway for maleate in Escherichia coli

  Shuhei Noda, Tomokazu Shirai, Yutaro Mori, Sachiko Oyama, Akihiko Kondo

  Oct. 2017, NATURE COMMUNICATIONS, 8(1) (1), 1153, English

  [Refereed]

  Scientific journal


• Quantitative metabolomics of a xylose-utilizing Saccharomyces cerevisiae strain expressing the Bacteroides thetaiotaomicron xylose isomerase on glucose and xylose

  M. J. Mert, S. H. Rose, D. C. la Grange, T. Bamba, T. Hasunuma, A. Kondo, W. H. van Zyl

  Oct. 2017, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 44(10) (10), 1459 - 1470, English

  [Refereed]

  Scientific journal


• Beyond Native Cas9: Manipulating Genomic Information and Function

  Hitoshi Mitsunobu, Jun Teramoto, Keiji Nishida, Akihiko Kondo

  Oct. 2017, TRENDS IN BIOTECHNOLOGY, 35(10) (10), 983 - 996, English

  [Refereed]

  Scientific journal


• Editorial Special Issue on the Occasion of Prof. Dr. Alf Pühler's 75th birthday

  Paul Marques Alves, Anke Becker, Helmut Blöcker, Raquel Lia Chan, George Guo-Qiang Chen, Martin Fussenegger, Akihiko Kondo, Robert Kourist, Helmut Schwab, Jin-Ho Seo, Christoph W. Sensen, Maria Sensen, Friedrich Scrienc

  Elsevier B.V., Sep. 2017, Journal of Biotechnology, 257, 1, English

  [Refereed]

  Scientific journal


• Variety in excitation energy transfer processes from phycobilisomes to photosystems I and II

  Yoshifumi Ueno, Shimpei Aikawa, Kyosuke Niwa, Tomoko Abe, Akio Murakami, Akihiko Kondo, Seiji Akimoto

  Sep. 2017, PHOTOSYNTHESIS RESEARCH, 133(1-3) (1-3), 235 - 243, English

  [Refereed]

  Scientific journal


• Energy transfer in Anabaena variabilis filaments adapted to nitrogen-depleted and nitrogen-enriched conditions studied by time-resolved fluorescence

  Aya Onishi, Shimpei Aikawa, Akihiko Kondo, Seiji Akimoto

  Sep. 2017, PHOTOSYNTHESIS RESEARCH, 133(1-3) (1-3), 317 - 326, English

  [Refereed]

  Scientific journal


• Heterologous expression of bacterial phosphoenol pyruvate carboxylase and Entner-Doudoroff pathway in Saccharomyces cerevisiae for improvement of isobutanol production

  Keisuke Morita, Yuta Nomura, Jun Ishii, Fumio Matsuda, Akihiko Kondo, Hiroshi Shimizu

  Sep. 2017, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 124(3) (3), 263 - 270, English

  [Refereed]

  Scientific journal


• Recent Advances in Microbial Production of Aromatic Chemicals and Derivatives

  Shuhei Noda, Akihiko Kondo

  Aug. 2017, TRENDS IN BIOTECHNOLOGY, 35(8) (8), 785 - 796, English

  [Refereed]

  Scientific journal


• Sucrose purification and repeated ethanol production from sugars remaining in sweet sorghum juice subjected to a membrane separation process.

  Kengo Sasaki, Yota Tsuge, Hideo Kawaguchi, Masahiro Yasukawa, Daisuke Sasaki, Takashi Sazuka, Eiji Kamio, Chiaki Ogino, Hideto Matsuyama, Akihiko Kondo

  The juice from sweet sorghum cultivar SIL-05 (harvested at physiological maturity) was extracted, and the component sucrose and reducing sugars (such as glucose and fructose) were subjected to a membrane separation process to purify the sucrose for subsequent sugar refining and to obtain a feedstock for repeated bioethanol production. Nanofiltration (NF) of an ultrafiltration (UF) permeate using an NTR-7450 membrane (Nitto Denko Corporation, Osaka, Japan) concentrated the juice and produced a sucrose-rich fraction (143.2 g L-1 sucrose, 8.5 g L-1 glucose, and 4.5 g L-1 fructose). In addition, the above NF permeate was concentrated using an ESNA3 NF membrane to provide concentrated permeated sugars (227.9 g L-1) and capture various amino acids in the juice, enabling subsequent ethanol fermentation without the addition of an exogenous nitrogen source. Sequential batch fermentation using the ESNA3 membrane concentrate provided an ethanol titer and theoretical ethanol yield of 102.5-109.5 g L-1 and 84.4-89.6%, respectively, throughout the five-cycle batch fermentation by Saccharomyces cerevisiae BY4741. Our results demonstrate that a membrane process using UF and two types of NF membranes has the potential to allow sucrose purification and repeated bioethanol production.

  Aug. 2017, Applied microbiology and biotechnology, 101(15) (15), 6007 - 6014, English, International magazine

  [Refereed]

  Scientific journal


• Metabolic engineering of Schizosaccharomyces pombe via CRISPR-Cas9 genome editing for lactic acid production from glucose and cellobiose

  OZAKI Aiko, KONISHI Rie, OTOMO Chisako, KISHIDA Mayumi, TAKAYAMA Seiya, MATSUMOTO Takuya, TANAKA Tsutomu, KONDO Akihiko

  Aug. 2017, Metabolic Engineering Communications, 5, 60 - 67, English

  [Refereed]

  Scientific journal


• Development of a comprehensive set of tools for genome engineering in a cold- and thermo-tolerant Kluyveromyces marxianus yeast strain

  Yumiko Nambu-Nishida, Keiji Nishida, Tomohisa Hasunuma, Akihiko Kondo

  Aug. 2017, SCIENTIFIC REPORTS, 7(1) (1), 8993, English

  [Refereed]

  Scientific journal


• Caffeic acid production by simultaneous saccharification and fermentation of kraft pulp using recombinant Escherichia coli

  Hideo Kawaguchi, Yohei Katsuyama, Du Danyao, Prihardi Kahar, Sachiko Nakamura-Tsuruta, Hiroshi Teramura, Keiko Wakai, Kumiko Yoshihara, Hiromichi Minami, Chiaki Ogino, Yasuo Ohnishi, Akihiko Kondo

  Jul. 2017, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 101(13) (13), 5279 - 5290, English

  [Refereed]

  Scientific journal


• Targeted Nucleotide Substitution in Mammalian Cell by Target-AID

  Akihiko Kondo

  Jun. 2017, Bio-Protocol, 7, 11, English

  [Refereed]

  Scientific journal


• Split luciferase complementation assay for the analysis of G protein-coupled receptor ligand response in Saccharomyces cerevisiae

  Yosuke Fukutani, Jun Ishii, Akihiko Kondo, Takeaki Ozawa, Hiroaki Matsunami, Masafumi Yohda

  Jun. 2017, BIOTECHNOLOGY AND BIOENGINEERING, 114(6) (6), 1354 - 1361, English

  [Refereed]

  Scientific journal


• Positive Feedback Genetic Circuit Incorporating a Constitutively Active Mutant Gal3 into Yeast GAL Induction System

  Shintaro Ryo, Jun Ishii, Toshihide Matsimo, Yasuyuki Nakamura, Daiki Matsubara, Masahiro Tominaga, Akihiko Kondo

  Jun. 2017, ACS SYNTHETIC BIOLOGY, 6(6) (6), 928 - 935, English

  [Refereed]

  Scientific journal


• Improvement of ethanol production from crystalline cellulose via optimizing cellulase ratios in cellulolytic Saccharomyces cerevisiae

  Zhuo Liu, Kentaro Inokuma, Shih-Hsin Ho, Riaan den Haan, Willem H. van Zyl, Tomohisa Hasunuma, Akihiko Kondo

  Jun. 2017, BIOTECHNOLOGY AND BIOENGINEERING, 114(6) (6), 1201 - 1207, English

  [Refereed]

  Scientific journal


• Direct Ethanol Production from Ionic Liquid-Pretreated Lignocellulosic Biomass by Cellulase-Displaying Yeasts

  Ryosuke Yamada, Kazunori Nakashima, Nanami Asai-Nakashima, Wataru Tokuhara, Nobuhiro Ishida, Satoshi Katahira, Noriho Kamiya, Chiaki Ogino, Akihiko Kondo

  May 2017, APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 182(1) (1), 229 - 237, English

  [Refereed]

  Scientific journal


• Up-concentration of sugars in pretreated-rice straw by an osmotic pressure-driven method

  Masafumi Shibuya, Masahiro Yasukawa, Kengo Sasaki, Yasuhiro Tanaka, Tomoki Takahashi, Akihiko Kondo, Hideto Matsuyama

  May 2017, BIOCHEMICAL ENGINEERING JOURNAL, 121, 13 - 16, English

  [Refereed]

  Scientific journal


• Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

  Zenpei Shimatani, Sachiko Kashojiya, Mariko Takayama, Rie Terada, Takayuki Arazoe, Hisaki Ishii, Hiroshi Teramura, Tsuyoshi Yamamoto, Hiroki Komatsu, Kenji Miura, Hiroshi Ezura, Keiji Nishida, Tohru Ariizumi, Akihiko Kondo

  May 2017, NATURE BIOTECHNOLOGY, 35(5) (5), 441 - +, English

  [Refereed]

  Scientific journal


• Dynamic metabolic profiling together with transcription analysis reveals salinity-induced starch-to-lipid biosynthesis in alga Chlamydomonas sp JSC4

  Shih-Hsin Ho, Akihito Nakanishi, Yuichi Kato, Hiroaki Yamasaki, Jo-Shu Chang, Naomi Misawa, Yuu Hirose, Jun Minagawa, Tomohisa Hasunuma, Akihiko Kondo

  Apr. 2017, SCIENTIFIC REPORTS, 7(7) (7), 45471, English

  [Refereed]

  Scientific journal


• Recent insights into biohydrogen production by microalgae - From biophotolysis to dark fermentation

  Dillirani Nagarajan, Duu-Jong Lee, Akihiko Kondo, Jo-Shu Chang

  Mar. 2017, BIORESOURCE TECHNOLOGY, 227, 373 - 387, English

  [Refereed]

  Scientific journal


• High-Speed Scanning for the Quantitative Evaluation of Glycogen Concentration in Bioethanol Feedstock Synechocystis sp PCC6803 Using a Near-Infrared Hyperspectral Imaging System with a New Near-Infrared Spectral Camera

  Mika Ishigaki, Akihito Nakanishi, Tomohisa Hasunuma, Akihiko Kondo, Tetsu Morishima, Toshiaki Okuno, Yukihiro Ozaki

  Mar. 2017, APPLIED SPECTROSCOPY, 71(3) (3), 463 - 471, English

  [Refereed]

  Scientific journal


• Yield Optimisation of Hepatitis B Virus Core Particles in E-coli Expression System for Drug Delivery Applications

  Izzat Fahimuddin Bin Mohamed Suffian, Mitla Garcia-Maya, Paul Brown, Tam Bui, Yuya Nishimura, Amir Rafiq Bin Mohammad Johari Palermo, Chiaki Ogino, Akihiko Kondo, Khuloud T. Al-Jamal

  Mar. 2017, SCIENTIFIC REPORTS, 7, 43160, English

  [Refereed]

  Scientific journal


• Simultaneous conversion of free fatty acids and triglycerides to biodiesel by immobilized Aspergillus oryzae expressing Fusarium heterosporum lipase

  Jerome Amoah, Emmanuel Quayson, Shinji Hama, Ayumi Yoshida, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Mar. 2017, BIOTECHNOLOGY JOURNAL, 12(3) (3), 1600400, English

  [Refereed]

  Scientific journal


• Production of optically pure D-lactic acid from brown rice using metabolically engineered Lactobacillus plantarum

  Kenji Okano, Shinji Hama, Maki Kihara, Hideo Noda, Tsutomu Tanaka, Akihiko Kondo

  Mar. 2017, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 101(5) (5), 1869 - 1875, English

  [Refereed]

  Scientific journal


• Mapping of endoglucanases displayed on yeast cell surface using atomic force microscopy

  Musashi Takenaka, Takuya Kobayashi, Kentaro Inokuma, Tomohisa Hasunuma, Tatsuo Maruyama, Chiaki Ogino, Akihiko Kondo

  Mar. 2017, COLLOIDS AND SURFACES B-BIOINTERFACES, 151, 134 - 142, English

  [Refereed]

  Scientific journal


• Enzymatic improvement of mitochondrial thiol oxidase Erv1 for oxidized glutathione fermentation by Saccharomyces cerevisiae

  Jyumpei Kobayashi, Daisuke Sasaki, Kiyotaka Y. Hara, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2017, MICROBIAL CELL FACTORIES, 16, 44, English

  [Refereed]

  Scientific journal


• Engineering hepatitis B virus core particles for targeting HER2 receptors in vitro and in vivo

  Izzat Fahimuddin Bin Mohamed Suffian, Julie Tzu-Wen Wang, Naomi O. Hodgins, Rebecca Klippstein, Mitla Garcia-Maya, Paul Brown, Yuya Nishimura, Hamed Heidari, Sara Bals, Jane K. Sosabowski, Chiaki Ogino, Akihiko Kondo, Khuloud T. Al-Jamal

  Mar. 2017, BIOMATERIALS, 120, 126 - 138, English

  [Refereed]

  Scientific journal


• A Systematic Approach to Timeseries Metabolite Profiling and RNA-seq Analysis of Chinese Hamster Ovary Cell Culture

  Han-Hsiu Hsu, Michihiro Araki, Masao Mochizuki, Yoshimi Hori, Masahiro Murata, Prihardi Kahar, Takanobu Yoshida, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2017, SCIENTIFIC REPORTS, 7, 43518, English

  [Refereed]

  Scientific journal


• DNA-duplex linker for AFM-SELEX of DNA aptamer against human serum albumin

  Musashi Takenaka, Yuzo Okumura, Tomokazu Amino, Yusuke Miyachi, Chiaki Ogino, Akihiko Kondo

  Feb. 2017, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 27(4) (4), 954 - 957, English

  [Refereed]

  Scientific journal


• Application of THz Vibrational Spectroscopy to Molecular Characterization and the Theoretical Fundamentals: An Illustration Using Saccharide Molecules

  Feng Zhang, Houng-Wei Wang, Keisuke Tominaga, Michitoshi Hayashi, Tomohisa Hasunuma, Akihiko Kondo

  Feb. 2017, CHEMISTRY-AN ASIAN JOURNAL, 12(3) (3), 324 - 331, English

  [Refereed]

  Scientific journal


• Application of LC-MS/MS analysis for time-lapse amino acid metabolomics in CHO cell culture.

  Hsu H, Hasunuma T, Araki M, Yoshida T, Hori Y, Murata M, Kondo A

  Feb. 2017, Shimadzu Journal, 5(1) (1), 17 - 21, English

  [Refereed]

  Scientific journal


• The clinical profile of patients with cardiac arrest induced by hemorrhagic stroke

  Akihiko Kondo

  2017, Resuscitation

  Scientific journal


• PREFACE: Special Issue on Advances in Industrial Bioprocesses and Products - Genetic and Metabolic Engineering Interventions

  Akihiko Kondo

  2017, Bioresource Technology

  Scientific journal


• Reversible amygdala enlargement: a longitudinal observation of a patient with elderly onset temporal lobe epilepsy

  Akihiko Kondo

  2017, Journal of Neurology

  Scientific journal


• Kounis syndrome should be excluded when physicians treat patients with anaphylaxis

  Akihiko Kondo

  2017, Annals of Allergy, Asthma & Immunology

  Scientific journal


• Efficacy of Fiber Tractography in the Stereotactic Surgery of the Thalamus for Patients with Essential Tremor

  Akihiko Kondo

  2017, Neurologia Medico-Chirurgica

  Scientific journal


• Clinical profile of patients with cardiac arrest induced by aortic disease

  Akihiko Kondo

  2017, Resuscitation

  Scientific journal


• Characteristics of EEG Seizure-Onset Patterns Recorded From Subdural Electrodes Over MRI-Visible Frontal Focal Cortical Dysplasia Type IIb Lesions

  Akihiko Kondo

  2017, Journal of Clinical Neurophysiology

  Scientific journal


• O-19 Development of the novel yeast cell-surface display cassette for consolidated bioethanol production from biomass resources

  INOKUMA Kentaro, HASUNUMA Tomohisa, KONDO Akihiko

  The recombinant yeast strains displaying the heterologous cellulolytic enzymes on the cell surface using the glycosylphosphatidylinositol (GPI) anchoring system are considered promising biocatalysts for the consolidated bioethanol production from lignocellulosic biomass. In this study, we constructed novel gene cassettes for the efficient cellulase display on yeast cell surface. We revealed that simultaneous utilization of the GPI anchoring region derived from Saccharomyces cerevisiae SED1 and its original promoter in a gene cassette enabled highly-efficient enzyme integration into the cell wall. The β-glucosidase and endoglucanase activities of recombinant yeast cells transduced with the novel gene cassette were 8.4- and 106-fold higher than those of conventional strains. The novel gene cassette also improved cell-surface hemicellulase activity. These results suggest that the novel gene cassette has the wide applicability for efficient cell-surface display of heterologous enzymes and that recombinant yeast cells displaying enzymes using these cassettes are promising biocatalysts for the efficient ethanol production from biomass resources.

  The Japan Institute of Energy, 2017, Proceedings of the Conference on Biomass Science, 12, 37 - 38, Japanese


• Feasibility of CO2 mitigation and carbohydrate production by microalga Scenedesmus obliquus CNW-N used for bioethanol fermentation under outdoor conditions: effects of seasonal changes

  Shih-Hsin Ho, Yi-Di Chen, Ching-Yu Chang, Yen-Ying Lai, Chun-Yen Chen, Akihiko Kondo, Nan-Qi Ren, Jo-Shu Chang

  Jan. 2017, BIOTECHNOLOGY FOR BIOFUELS, 10, 27, English

  [Refereed]

  Scientific journal


• Development of combined nanofiltration and forward osmosis process for production of ethanol from pretreated rice straw

  Shibuya, M., Sasaki, K., Tanaka, Y., Yasukawa, M., Takahashi, T., Kondo, A., Matsuyama, H.

  2017, Bioresource Technology, 235, 405 - 410, English

  [Refereed]

  Scientific journal


• Erratum for Inokuma et al., Complete genome sequence of Kluyveromyces marxianus NBRC1777, a nonconventional thermotolerant yeast [3, 2, e00389-15 (1-1) (2015)]

  Kentaro Inokuma, Jun Ishii, Kiyotaka Y. Hara, Masao Mochizuki, Tomohisa Hasunuma, Akihiko Kondo

  American Society for Microbiology, 2017, Genome Announcements, 5(5) (5), English

  [Refereed]

  Scientific journal


• Taurine does not affect the composition, diversity, or metabolism of human colonic microbiota simulated in a single-batch fermentation system.

  Kengo Sasaki, Daisuke Sasaki, Naoko Okai, Kosei Tanaka, Ryohei Nomoto, Itsuko Fukuda, Ken-Ichi Yoshida, Akihiko Kondo, Ro Osawa

  Accumulating evidence suggests that dietary taurine (2-aminoethanesulfonic acid) exerts beneficial anti-inflammatory effects in the large intestine. In this study, we investigated the possible impact of taurine on human colonic microbiota using our single-batch fermentation system (Kobe University Human Intestinal Microbiota Model; KUHIMM). Fecal samples from eight humans were individually cultivated with and without taurine in the KUHIMM. The results showed that taurine remained largely undegraded after 30 h of culturing in the absence of oxygen, although some 83% of the taurine was degraded after 30 h of culturing under aerobic conditions. Diversity in bacterial species in the cultures was analyzed by 16S rRNA gene sequencing, revealing that taurine caused no significant change in the diversity of the microbiota; both operational taxonomic unit and Shannon-Wiener index of the cultures were comparable to those of the respective source fecal samples. In addition, principal coordinate analysis indicated that taurine did not alter the composition of bacterial species, since the 16S rRNA gene profile of bacterial species in the original fecal sample was maintained in each of the cultures with and without taurine. Furthermore, metabolomic analysis revealed that taurine did not affect the composition of short-chain fatty acids produced in the cultures. These results, under these controlled but artificial conditions, suggested that the beneficial anti-inflammatory effects of dietary taurine in the large intestine are independent of the intestinal microbiota. We infer that dietary taurine may act directly in the large intestine to exert anti-inflammatory effects.

  2017, PloS one, 12(7) (7), e0180991, English, International magazine

  [Refereed]

  Scientific journal


• Affibody-displaying bio-nanocapsules effective in EGFR, typical biomarker, expressed in various cancer cells

  Yuya Nishimura, Ryosuke Ezawa, Jun Ishii, Chiaki Ogino, Akihiko Kondo

  Jan. 2017, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 27(2) (2), 336 - 341, English

  [Refereed]

  Scientific journal


• Differences in glucose yield of residues from among varieties of rice, wheat, and sorghum after dilute acid pretreatment

  Hiroshi Teramura, Kengo Sasaki, Hideo Kawaguchi, Fumio Matsuda, Jun Kikuchi, Tomokazu Shirai, Takashi Sazuka, Masanori Yamasaki, Shigeo Takumi, Chiaki Ogino, Akihiko Kondo

  2017, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 81(8) (8), 1650 - 1656, English

  [Refereed]

  Scientific journal


• Overexpression of CO2-responsive CCT protein, a key regulator of starch synthesis strikingly increases the glucose yield from rice straw for bioethanol production

  Ryutaro Morita, Hiroshi Teramura, Chiaki Ogino, Akihiko Kondo, Hiroshi Fukayama

  2017, PLANT PRODUCTION SCIENCE, 20(4) (4), 441 - 447, English

  [Refereed]

  Scientific journal


• Styrene production from a biomass-derived carbon source using a coculture system of phenylalanine ammonia lyase and phenylacrylic acid decarboxylase-expressing Streptomyces lividans transformants

  Ryosuke Fujiwara, Shuhei Noda, Tsutomu Tanaka, Akihiko Kondo

  Dec. 2016, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 122(6) (6), 730 - 735, English

  [Refereed]

  Scientific journal


• Comprehension of an organosolv process for lignin extraction on Festuca arundinacea and monitoring of the cellulose degradation

  Quentin Schmetz, Guillaume Maniet, Nicolas Jacquet, Hiroshi Teramura, Chiaki Ogino, Akihiko Kondo, Aurore Richel

  Dec. 2016, INDUSTRIAL CROPS AND PRODUCTS, 94, 308 - 317, English

  [Refereed]

  Scientific journal


• Succinate and Lactate Production from Euglena gracilis during Dark, Anaerobic Conditions

  Yuko Tomita, Kazumasa Yoshioka, Hiroko Iijima, Ayaka Nakashima, Osamu Iwata, Kengo Suzuki, Tomohisa Hasunuma, Akihiko Kondo, Masami Yokota Hirai, Takashi Osanai

  Dec. 2016, FRONTIERS IN MICROBIOLOGY, 7, 2050, English

  [Refereed]

  Scientific journal


• Continuous crossbreeding of sake yeasts using growth selection systems for a-type and α-type cells

  Nobuo Fukuda, Misato Kaishima, Jun Ishii, Akihiko Kondo, Shinya Honda

  Springer Verlag, Dec. 2016, AMB Express, 6(1) (1), 45, English

  [Refereed]

  Scientific journal


• Comparative metabolic state of microflora on the surface of the anode electrode in a microbial fuel cell operated at different pH conditions

  Daisuke Sasaki, Kengo Sasaki, Yota Tsuge, Akihiko Kondo

  Dec. 2016, AMB EXPRESS, 6, 125, English

  [Refereed]

  Scientific journal


• Characterization of titanium dioxide nanoparticles modified with polyacrylic acid and H2O2 for use as a novel radiosensitizer

  Kenta Morita, Serika Miyazaki, Chiya Numako, Shinya Ikeno, Ryohei Sasaki, Yuya Nishimura, Chiaki Ogino, Akihiko Kondo

  Dec. 2016, FREE RADICAL RESEARCH, 50(12) (12), 1319 - 1328, English

  [Refereed]

  Scientific journal


• Bioprocessing of bio-based chemicals produced from lignocellulosic feedstocks

  Hideo Kawaguchi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Dec. 2016, CURRENT OPINION IN BIOTECHNOLOGY, 42, 30 - 39, English

  [Refereed]

  Scientific journal


• Production of fuels and chemicals from biomass by integrated bioprocesses

  Tomohisa Hasunuma, Akihiko Kondo

  Wiley-VCH Verlag, Nov. 2016, Industrial Biotechnology: Products and Processes, 161 - 185, English

  [Refereed]

  In book


• Sortase A-Mediated Metabolic Enzyme Ligation in Escherichia coli

  Takuya Matsumoto, Kou Furuta, Tsutomu Tanaka, Akihiko Kondo

  Nov. 2016, ACS SYNTHETIC BIOLOGY, 5(11) (11), 1284 - 1289, English

  [Refereed]

  Scientific journal


• Enhanced Cell-Surface Display and Secretory Production of Cellulolytic Enzymes With Saccharomyces cerevisiae Sed1 Signal Peptide

  Kentaro Inokuma, Takahiro Bamba, Jun Ishii, Yoichiro Ito, Tomohisa Hasunuma, Akihiko Kondo

  Nov. 2016, BIOTECHNOLOGY AND BIOENGINEERING, 113(11) (11), 2358 - 2366, English

  [Refereed]

  Scientific journal


• Dual-Color Reporter Switching System to Discern Dimer Formations of G-Protein-Coupled Receptors Using Cre/loxP Site-Specific Recombination in Yeast

  Yasuyuki Nakamura, Takamichi Hashimoto, Jun Ishii, Akihiko Kondo

  Oct. 2016, BIOTECHNOLOGY AND BIOENGINEERING, 113(10) (10), 2178 - 2190, English

  [Refereed]

  Scientific journal


• Ethanol production from N-acetyl-D-glucosamine by Scheffersomyces stipitis strains

  Kentaro Inokuma, Tomohisa Hasunuma, Akihiko Kondo

  Oct. 2016, AMB EXPRESS, 6, 83, English

  [Refereed]

  Scientific journal


• Energy Transfer in Cyanobacteria and Red Algae: Confirmation of Spillover in Intact Megacomplexes of Phycobilisome and Both Photosystems

  Yoshifumi Ueno, Shimpei Aikawa, Akihiko Kondo, Seiji Akimoto

  Sep. 2016, JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 7(18) (18), 3567 - 3571, English

  [Refereed]

  Scientific journal


• 4-Vinylphenol production from glucose using recombinant Streptomyces mobaraense expressing a tyrosine ammonia lyase from Rhodobacter sphaeroides

  Ryosuke Fujiwara, Shuhei Noda, Yoshifumi Kawai, Tsutomu Tanaka, Akihiko Kondo

  Sep. 2016, BIOTECHNOLOGY LETTERS, 38(9) (9), 1543 - 1549, English

  [Refereed]

  Scientific journal


• Toward the complete utilization of rice straw: Methane fermentation and lignin recovery by a combinational process involving mechanical milling, supporting material and nanofiltration

  Kengo Sasaki, Mami Okamoto, Tomokazu Shirai, Yota Tsuge, Ayami Fujino, Daisuke Sasaki, Masahiko Morita, Fumio Matsuda, Jun Kikuchi, Akihiko Kondo

  Sep. 2016, BIORESOURCE TECHNOLOGY, 216, 830 - 837, English

  [Refereed]

  Scientific journal


• Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems

  Keiji Nishida, Takayuki Arazoe, Nozomu Yachie, Satomi Banno, Mika Kakimoto, Mayura Tabata, Masao Mochizuki, Aya Miyabe, Michihiro Araki, Kiyotaka Y. Hara, Zenpei Shimatani, Akihiko Kondo

  Sep. 2016, SCIENCE, 353(6305) (6305), aaf8729, English

  [Refereed]

  Scientific journal


• Recent advances in yeast cell-surface display technologies for waste biorefineries

  Zhuo Liu, Shih-Hsin Ho, Tomohisa Hasunuma, Jo-Shu Chang, Nan-Qi Ren, Akihiko Kondo

  Sep. 2016, BIORESOURCE TECHNOLOGY, 215, 324 - 333, English

  [Refereed]

  Scientific journal


• Metabolomics-based analysis revealing the alteration of primary carbon metabolism by the genetic manipulation of a hydrogenase HoxH in Synechocystis sp PCC 6803

  Hiroko Iijima, Tomokazu Shirai, Mami Okamoto, Filipe Pinto, Paula Tamagnini, Tomohisa Hasunuma, Akihiko Kondo, Masami Yokota Hirai, Takashi Osanai

  Sep. 2016, ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 18, 305 - 313, English

  [Refereed]

  Scientific journal


• From mannan to bioethanol: cell surface co-display of beta-mannanase and beta-mannosidase on yeast Saccharomyces cerevisiae

  Jun Ishii, Fumiyoshi Okazaki, Apridah Cameliawati Djohan, Kiyotaka Y. Hara, Nanami Asai-Nakashima, Hiroshi Teramura, Ade Andriani, Masahiro Tominaga, Satoshi Wakai, Prihardi Kahar, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

  Sep. 2016, BIOTECHNOLOGY FOR BIOFUELS, 9(1) (1), 188, English

  [Refereed]

  Scientific journal


• Enhancement of astaxanthin production in Xanthophyllomyces dendrorhous by efficient method for the complete deletion of genes

  Keisuke Yamamoto, Kiyotaka Y. Hara, Toshihiko Morita, Akira Nishimura, Daisuke Sasaki, Jun Ishii, Chiaki Ogino, Noriyuki Kizaki, Akihiko Kondo

  Sep. 2016, MICROBIAL CELL FACTORIES, 15(1) (1), 155, English

  [Refereed]

  Scientific journal


• Improving carbohydrate production of Chlorella sorokiniana NIES-2168 through semi-continuous process coupled with mixotrophic cultivation

  Yue Wang, Sheng-Yi Chiu, Shih-Hsin Ho, Zhuo Liu, Tomohisa Hasunuma, Ting-Ting Chang, Kuan-Fu Chang, Jo-Shu Chang, Nan-Qi Ren, Akihiko Kondo

  Aug. 2016, BIOTECHNOLOGY JOURNAL, 11(8) (8), 1072 - 1081, English

  [Refereed]

  Scientific journal


• Anionic metabolite biosynthesis enhanced by potassium under dark, anaerobic conditions in cyanobacteria

  Sakiko Ueda, Yuhki Kawamura, Hiroko Iijima, Mitsuharu Nakajima, Tomokazu Shirai, Mami Okamoto, Akihiko Kondo, Masami Yokota Hirai, Takashi Osanai

  Aug. 2016, SCIENTIFIC REPORTS, 6, 32354, English

  [Refereed]

  Scientific journal


• Improving carbohydrate production of Chlorella sorokiniana NIES-2168 through semi-continuous process coupled with mixotrophic cultivation

  Yue Wang, Sheng-Yi Chiu, Shih-Hsin Ho, Zhuo Liu, Tomohisa Hasunuma, Ting-Ting Chang, Kuan-Fu Chang, Jo-Shu Chang, Nan-Qi Ren, Akihiko Kondo

  Aug. 2016, BIOTECHNOLOGY JOURNAL, 11(8) (8), 1072 - 1081, English

  [Refereed]

  Scientific journal


• A Single-Batch Fermentation System to Simulate Human Colonic Microbiota for High-Throughput Evaluation of Prebiotics

  Risa Takagi, Kengo Sasaki, Daisuke Sasaki, Itsuko Fukuda, Kosei Tanaka, Ken-ichi Yoshida, Akihiko Kondo, Ro Osawa

  Aug. 2016, PLOS ONE, 11(8) (8), e0160533, English

  [Refereed]

  Scientific journal


• Improvement of ectoine productivity by using sugar transporter-overexpressing Halomonas elongata

  Kosuke Tanimura, Takuya Matsumoto, Hideki Nakayama, Tsutomu Tanaka, Akihiko Kondo

  Jul. 2016, ENZYME AND MICROBIAL TECHNOLOGY, 89, 63 - 68, English

  [Refereed]

  Scientific journal


• 2,3-Butanediol production from cellobiose using exogenous beta-glucosidase-expressing Bacillus subtilis

  Kosuke Tanimura, Shingo Takashima, Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo

  Jul. 2016, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 100(13) (13), 5781 - 5789, English

  [Refereed]

  Scientific journal


• Titanium peroxide nanoparticles enhanced cytotoxic effects of X-ray irradiation against pancreatic cancer model through reactive oxygen species generation in vitro and in vivo

  Masao Nakayama, Ryohei Sasaki, Chiaki Ogino, Tsutomu Tanaka, Kenta Morita, Mitsuo Umetsu, Satoshi Ohara, Zhenquan Tan, Yuya Nishimura, Hiroaki Akasaka, Kazuyoshi Sato, Chiya Numako, Seiichi Takami, Akihiko Kondo

  Jul. 2016, RADIATION ONCOLOGY, 11(1) (1), 91, English

  [Refereed]

  Scientific journal


• Overexpressing enzymes of the Ehrlich pathway and deleting genes of the competing pathway in Saccharomyces cerevisiae for increasing 2-phenylethanol production from glucose

  Li Shen, Yuya Nishimura, Fumio Matsuda, Jun Ishii, Akihiko Kondo

  Jul. 2016, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 122(1) (1), 34 - 39, English

  [Refereed]

  Scientific journal


• Lipase cocktail for efficient conversion of oils containing phospholipids to biodiesel

  Jerome Amoah, Shih-Hsin Ho, Shinji Hama, Ayumi Yoshida, Akihito Nakanishi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Jul. 2016, BIORESOURCE TECHNOLOGY, 211, 224 - 230, English

  [Refereed]

  Scientific journal


• Expression of varied GFPs in Saccharomyces cerevisiae: codon optimization yields stronger than expected expression and fluorescence intensity

  Akihiko Kondo

  Jun. 2016, Scientific Reports, 6, 35932, English

  [Refereed]

  Scientific journal


• Natural variation in the glucose content of dilute sulfuric acid-pretreated rice straw liquid hydrolysates: implications for bioethanol production

  Takashi Goda, Hiroshi Teramura, Miki Suehiro, Kengo Kanamaru, Hideo Kawaguchi, Chiaki Ogino, Akihiko Kondo, Masanori Yamasaki

  May 2016, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 80(5) (5), 863 - 869, English

  [Refereed]

  Scientific journal


• Improved sugar-free succinate production by Synechocystis sp. PCC 6803 following identification of the limiting steps in glycogen catabolism.

  Akihiko Kondo

  May 2016, Metabolic Engineering Communications, 3, 130 - 141, English

  [Refereed]

  Scientific journal


• Identification of a novel hedycaryol synthase gene isolated from Camellia brevistyla flowers and floral scent of Camellia cultivars

  Jun-ichiro Hattan, Kazutoshi Shindo, Tomoko Ito, Yurica Shibuya, Arisa Watanabe, Chie Tagaki, Fumina Ohno, Tetsuya Sasaki, Jun Ishii, Akihiko Kondo, Norihiko Misawa

  Apr. 2016, PLANTA, 243(4) (4), 959 - 972, English

  [Refereed]

  Scientific journal


• Engineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel production

  Zhuo Liu, Shih-Hsin Ho, Kengo Sasaki, Riaan den Haan, Kentaro Inokuma, Chiaki Ogino, Willem H. van Zyl, Tomohisa Hasunuma, Akihiko Kondo

  Apr. 2016, SCIENTIFIC REPORTS, 6, 24550, English

  [Refereed]

  Scientific journal


• Cell surface engineering of Saccharomyces cerevisiae combined with membrane separation technology for xylitol production from rice straw hydrolysate

  Gregory Guirimand, Kengo Sasaki, Kentaro Inokuma, Takahiro Bamba, Tomohisa Hasunuma, Akihiko Kondo

  Apr. 2016, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 100(8) (8), 3477 - 3487, English

  [Refereed]

  Scientific journal


• Twigged streptavidin polymer as a scaffold for protein assembly

  Akihiko Kondo

  Mar. 2016, Journal of Biotechnology, 255, 61 - 66, English

  [Refereed]

  Scientific journal


• FudC, a protein primarily responsible for furfural detoxification in Corynebacterium glutamicum

  Yota Tsuge, Motonori Kudou, Hideo Kawaguchi, Jun Ishii, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2016, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 100(6) (6), 2685 - 2692, English

  [Refereed]

  Scientific journal


• d-lactic acid production from renewable lignocellulosic biomass via genetically modified Lactobacillus plantarum

  Yixing Zhang, Amit Kumar, Philip R. Hardwidge, Tsutomu Tanaka, Akihiko Kondo, Praveen V. Vadlani

  Mar. 2016, BIOTECHNOLOGY PROGRESS, 32(2) (2), 271 - 278, English

  [Refereed]

  Scientific journal


• Disruption of PHO13 improves ethanol production via the xylose isomerase pathway

  Akihiko Kondo

  Mar. 2016, AMB Express, 6(1) (1), 4, English

  [Refereed]

  Scientific journal


• Organosolv pretreatment of sorghum bagasse using a low concentration of hydrophobic solvents such as 1-butanol or 1-pentanol

  Hiroshi Teramura, Kengo Sasaki, Tomoko Oshima, Fumio Matsuda, Mami Okamoto, Tomokazu Shirai, Hideo Kawaguchi, Chiaki Ogino, Ko Hirano, Takashi Sazuka, Hidemi Kitano, Jun Kikuchi, Akihiko Kondo

  Feb. 2016, BIOTECHNOLOGY FOR BIOFUELS, 9, 27, English

  [Refereed]

  Scientific journal


• Characterization of cellulose nanofiber sheets from different refining processes

  Kohji Nobuta, Hiroshi Teramura, Hiroaki Ito, Chizuru Hongo, Hideo Kawaguchi, Chiaki Ogino, Akihiko Kondo, Takashi Nishino

  Feb. 2016, CELLULOSE, 23(1) (1), 403 - 414, English

  [Refereed]

  Scientific journal


• Seizures with tonic posturing: Semiologic difference between supplementary sensorimotor area (SSMA) origin and extra-SSMA origin

  Akihiko Kondo

  2016, Epilepsia

  Scientific journal


• Maternal oral administration of osteocalcin protects offspring from metabolic impairment in adulthood

  Akihiko Kondo

  2016, Obesity

  Scientific journal


• Contrast Peak Density in Collateral Vessels May Be an Important Factor in Tissue Fate in Acute Ischemic Stroke

  Akihiko Kondo

  2016, Cerebrovascular Diseases

  Scientific journal


• Development of efficient cellulolytic enzyme display on the cell surface of the yeast Saccharomyces cerevisiae

  Akihiko Kondo

  2016, New Biotechnology

  Scientific journal


• Anti-high mobility group box 1 antibody exerts neuroprotection in a rat model of Parkinson's disease

  Akihiko Kondo

  2016, Experimental Neurology

  Scientific journal


• Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives

  Shuhei Noda, Tomokazu Shirai, Sachiko Oyama, Akihiko Kondo

  Jan. 2016, METABOLIC ENGINEERING, 33, 119 - 129, English

  [Refereed]

  Scientific journal


• Production of protocatechuic acid by Corynebacterium glutamicum expressing chorismate-pyruvate lyase from Escherichia coli.

  Naoko Okai, Takanori Miyoshi, Yasunobu Takeshima, Hiroaki Kuwahara, Chiaki Ogino, Akihiko Kondo

  Protocatechuic acid (3,4-dihydroxybenzoic acid; PCA) serves as a building block for polymers and pharmaceuticals. In this study, the biosynthetic pathway for PCA from glucose was engineered in Corynebacterium glutamicum. The pathway to PCA-employed elements of the chorismate pathway by using chorismate-pyruvate lyase (CPL) and 4-hydroxybenzoate hydroxylase (4-HBA hydroxylase). As C. glutamicum has the potential to synthesize the aromatic amino acid intermediate chorismate and possesses 4-HBA hydroxylase, we focused on expressing Escherichia coli CPL in a phenylalanine-producing strain of C. glutamicum ATCC21420. To secrete PCA, the gene (ubiC) encoding CPL from E. coli was expressed in C. glutamicum ATCC 21420 (strain F(UbiC)). The formation of 28.8 mg/L of extracellular 4-HBA (36 h) and 213 ± 29 mg/L of extracellular PCA (80 h) was obtained by the C. glutamicum strain F(UbiC) from glucose. The strain ATCC21420 was also found to produce extracellular PCA. PCA fermentation was performed using C. glutamicum strain F(UbiC) in a bioreactor at the optimized pH of 7.5. C. glutamicum F(UbiC) produced 615 ± 2.1 mg/L of PCA from 50 g/L of glucose after 72 h. Further, fed-batch fermentation of PCA by C. glutamicum F(UbiC) was performed with feedings of glucose every 24 h. The maximum production of PCA (1140.0 ± 11.6 mg/L) was achieved when 117.0 g/L of glucose was added over 96 h of fed-batch fermentation.

  Jan. 2016, Applied microbiology and biotechnology, 100(1) (1), 135 - 45, English, International magazine

  [Refereed]

  Scientific journal


• Designing intracellular metabolism for production of target compounds by introducing a heterologous metabolic reaction based on a Synechosystis sp 6803 genome-scale model

  Tomokazu Shirai, Takashi Osanai, Akihiko Kondo

  Jan. 2016, MICROBIAL CELL FACTORIES, 15(1) (1), 13, English

  [Refereed]

  Scientific journal


• O-18 Simultaneous saccharification and fermentation of lignocellulosic biomass for phenyllactate production by recombinant Escherichia coli

  川口,秀夫, 寺村,浩, 中村,聡子, 荻野,千秋, 原,清敬, 蓮沼,誠久, 老沼,研一, 高谷,直樹, 平野,恒, 佐塚,隆志, 北野,英己, 近藤,昭彦

  Sorghum bagasse pretreated with diluted acid, which was predominantly composed of glucan (59%) and xylan (7.2%), was used as a lignocellulosic feedstock to produce D-phenyllactic acid (PhLA) by a recombinant Escherichia coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens. Compared to filter paper hydrolysate, the PhLA yield was reduced by 35% during fermentation with enzymatic hydrolysate of sorghum bagasse as a carbon source, and metabolomics analysis revealed that intracellular levels of erythrose-4-phosphate and phosphoenolpyruvate and NAD(P)H regeneration for PhLA production from glucose markedly reduced. Compared to the separate hydrolysis and fermentation (SHF) with sorghum bagasse hydrolysate, simultaneous saccharification and fermentation (SSF) of sorghum bagasse under glucose limitation conditions yielded 4.8-fold more PhLA with less accumulation of eluted components of p-coumaric acid and aldehydes, which inhibited PhLA fermentation. These results suggest that gradual hydrolysis of sorghum bagasse during SSF reduces the accumulation of both glucose and fermentation inhibitors, collectively leading to increased PhLA yield.

  The Japan Institute of Energy, Jan. 2016, Proceedings of the Conference on Biomass Science, 11, 35 - 36, Japanese

  [Refereed]

  International conference proceedings


• Complete genome sequence of Kluyveromyces marxianus NBRC1777, a nonconventional thermotolerant yeast

  Kentaro Inokuma, Jun Ishii, Kiyotaka Y. Hara, Masao Mochizuki, Tomohisa Hasunuma, Akihiko Kondo

  American Society for Microbiology, 2016, Genome Announcements, 3(2) (2), e00389 - 15, English

  [Refereed]

  Scientific journal


• Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae

  Kengo Sasaki, Kiyotaka Y. Hara, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

  Jan. 2016, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 121(1) (1), 96 - 100, English

  [Refereed]

  Scientific journal


• Inverse metabolic engineering based on transient acclimation of yeast improves acid-containing xylose fermentation and tolerance to formic and acetic acids

  Tomohisa Hasunuma, Takatoshi Sakamoto, Akihiko Kondo

  Jan. 2016, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 100(2) (2), 1027 - 1038, English

  [Refereed]

  Scientific journal


• Enhanced D-lactic acid production from renewable resources using engineered Lactobacillus plantarum

  Yixing Zhang, Praveen V. Vadlani, Amit Kumar, Philip R. Hardwidge, Revathi Govind, Tsutomu Tanaka, Akihiko Kondo

  Jan. 2016, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 100(1) (1), 279 - 288, English

  [Refereed]

  Scientific journal


• Engineering cell factories for producing building block chemicals for bio-polymer synthesis

  Yota Tsuge, Hideo Kawaguchi, Kengo Sasaki, Akihiko Kondo

  Jan. 2016, MICROBIAL CELL FACTORIES, 15, 19, English

  [Refereed]

  Scientific journal


• Converting oils high in phospholipids to biodiesel using immobilized Aspergillus oryzae whole-cell biocatalysts expressing Fusarium heterosporum lipase

  Jerome Amoah, Shih-Hsin Ho, Shinji Hama, Ayumi Yoshida, Akihito Nakanishi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Jan. 2016, BIOCHEMICAL ENGINEERING JOURNAL, 105, 10 - 15, English

  [Refereed]

  Scientific journal


• Comprehensive metabolomic analyses of anode-respiring Geobacter sulfurreducens cells: The impact of anode-respiration activity on intracellular metabolite levels

  Jieun Song, Daisuke Sasaki, Kengo Sasaki, Souichiro Kato, Akihiko Kondo, Kazuhito Hashimoto, Shuji Nakanishi

  Jan. 2016, PROCESS BIOCHEMISTRY, 51(1) (1), 34 - 38, English

  [Refereed]

  Scientific journal


• Current Techniques for Studying Oligomer Formations of G-Protein-Coupled Receptors Using Mammalian and Yeast Cells

  Yasuyuki Nakamura, Jun Ishii, Akihiko Kondo

  2016, CURRENT MEDICINAL CHEMISTRY, 23(16) (16), 1638 - 1656, English

  [Refereed]

  Scientific journal


• Rational design and evolutional fine tuning of Saccharomyces cerevisiae for biomass breakdown

  Tomohisa Hasunuma, Jun Ishii, Akihiko Kondo

  Dec. 2015, CURRENT OPINION IN CHEMICAL BIOLOGY, 29, 1 - 9, English

  [Refereed]

  Scientific journal


• Construction of a Genome-Scale Metabolic Model of Arthrospira platensis NIES-39 and Metabolic Design for Cyanobacterial Bioproduction

  Katsunori Yoshikawa, Shimpei Aikawa, Yuta Kojima, Yoshihiro Toya, Chikara Furusawa, Akihiko Kondo, Hiroshi Shimizu

  Dec. 2015, PLOS ONE, 10(12) (12), e0144430, English

  [Refereed]

  Scientific journal


• ATP regulation in bioproduction

  Kiyotaka Y. Hara, Akihiko Kondo

  Dec. 2015, MICROBIAL CELL FACTORIES, 14, 198, English

  [Refereed]

  Scientific journal


• A pilot-scale floating closed culture system for the multicellular cyanobacterium Arthrospira platensis NIES-39

  Masakazu Toyoshima, Shimpei Aikawa, Takahiro Yamagishi, Akihiko Kondo, Hiroshi Kawai

  Dec. 2015, JOURNAL OF APPLIED PHYCOLOGY, 27(6) (6), 2191 - 2202, English

  [Refereed]

  Scientific journal


• 3-Amino-4-hydroxybenzoic acid production from sweet sorghum juice by recombinant Corynebacterium glutamicum

  Hideo Kawaguchi, Kengo Sasaki, Kouji Uematsu, Yota Tsuge, Hiroshi Teramura, Naoko Okai, Sachiko Nakamura-Tsuruta, Yohei Katsuyama, Yoshinori Sugai, Yasuo Ohnishi, Ko Hirano, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

  Dec. 2015, BIORESOURCE TECHNOLOGY, 198, 410 - 417, English

  [Refereed]

  Scientific journal


• G gamma recruitment systems specifically select PPI and affinity-enhanced candidate proteins that interact with membrane protein targets

  Misato Kaishima, Jun Ishii, Nobuo Fukuda, Akihiko Kondo

  Nov. 2015, SCIENTIFIC REPORTS, 5, 16723, English

  [Refereed]

  Scientific journal


• Cell surface engineering of industrial microorganisms for biorefining applications

  Akihiko Kondo

  Nov. 2015, Biotechnology Advances, 33, 1403 - 1411, English

  [Refereed]

  Scientific journal


• Unique Plasmids Generated via pUC Replicon Mutagenesis in an Error-Prone Thermophile Derived from Geobacillus kaustophilus HTA426

  Jyumpei Kobayashi, Misaki Tanabiki, Shohei Doi, Akihiko Kondo, Takashi Ohshiro, Hirokazu Suzuki

  Nov. 2015, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(21) (21), 7625 - 7632, English

  [Refereed]

  Scientific journal


• Improvement of oxidized glutathione fermentation by thiol redox metabolism engineering in Saccharomyces cerevisiae

  Kiyotaka Y. Hara, Naoko Aoki, Jyumpei Kobayashi, Kentaro Kiriyama, Keiji Nishida, Michihiro Araki, Akihiko Kondo

  Nov. 2015, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 99(22) (22), 9771 - 9778, English

  [Refereed]

  Scientific journal


• Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium

  Takashi Osanai, Tomokazu Shirai, Hiroko Iijima, Yuka Nakaya, Mami Okamoto, Akihiko Kondo, Masami Y. Hirai

  Oct. 2015, FRONTIERS IN MICROBIOLOGY, 6, 1064, English

  [Refereed]

  Scientific journal


• Expression of Cyanobacterial Acyl-ACP Reductase Elevates the Triacylglycerol Level in the Red Alga Cyanidioschyzon merolae

  Nobuko Sumiya, Yasuko Kawase, Jumpei Hayakawa, Mami Matsuda, Mami Nakamura, Atsuko Era, Kan Tanaka, Akihiko Kondo, Tomohisa Hasunuma, Sousuke Imamura, Shin-ya Miyagishima

  Oct. 2015, PLANT AND CELL PHYSIOLOGY, 56(10) (10), 1962 - 1980, English

  [Refereed]

  Scientific journal


• Enzymatic synthesis and modification of structured phospholipids: recent advances in enzyme preparation and biocatalytic processes

  Shinji Hama, Chiaki Ogino, Akihiko Kondo

  Oct. 2015, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 99(19) (19), 7879 - 7891, English

  [Refereed]

  Scientific journal


• C-Terminal-oriented Immobilization of Enzymes Using Sortase A-mediated Technique

  Yuto Hata, Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo

  Oct. 2015, MACROMOLECULAR BIOSCIENCE, 15(10) (10), 1375 - 1380, English

  [Refereed]

  Scientific journal


• バイオリファイナリーの現状と展望

  HASUNUMA TOMOHISA, ISHII JUN, OGINO CHIAKI, KONDO AKIHIKO

  持続可能な社会へ向かうためには再生可能エネルギーが中心的な役割を果たすことが求められている．そのなかで，バイオマスから液体燃料やバルクケミカルを経済性良く，高効率で生産する技術の開発が期待されている．バイオマスとしては，安定的な供給が可能で，食糧と競合しないリグノセルロース系バイオマスの利活用が望まれている．本稿ではリグノセルロース系バイオマスからのエタノールの製造プロセスについて研究の課題と最新の知見を紹介するとともに，バイオプロセスによるバルクケミカル生産に関する最近の研究例についても紹介する．

  Japan Society for Bioscience, Biotechnology, and Agrochemistry, Oct. 2015, 化学と生物, 53(10) (10), 689 - 695, Japanese

  Scientific journal


• 大腸菌パスウェイエンジニアリングによるパクリタキセル前駆体タキサジエン酸化物の生産

  竹村 秀史, 鈴木 宗典, 原田 尚志, 三沢 典彦, 石井 純, 近藤 昭彦, 播本 孝史

  (公社)日本生物工学会, Sep. 2015, 日本生物工学会大会講演要旨集, 平成27年度, 337 - 337, Japanese


• 合成生物工学によるモノづくり微生物のデザインに向けて

  ISHII JUN, ARAKI MICHIHIRO, NAKATSUI MASAHIKO, SAKIHAMA YURI, 柘植 陽太, HASUNUMA TOMOHISA, KONDO AKIHIKO

  Sep. 2015, 生物工学会誌, 93(9) (9), 523 - 526, Japanese

  Scientific journal


• Combined cell-surface display- and secretion-based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae

  Zhuo Liu, Kentaro Inokuma, Shih-Hsin Ho, Riaan den Haan, Tomohisa Hasunuma, Willem H. van Zyl, Akihiko Kondo

  Sep. 2015, BIOTECHNOLOGY FOR BIOFUELS, 8, 162, English

  [Refereed]

  Scientific journal


• Applications of yeast-based signaling sensor for characterization of antagonist and analysis of site-directed mutants of the human serotonin 1A receptor

  Yasuyuki Nakamura, Jun Ishii, Akihiko Kondo

  Sep. 2015, BIOTECHNOLOGY AND BIOENGINEERING, 112(9) (9), 1906 - 1915, English

  [Refereed]

  Scientific journal


• Light adaptation of the unicellular red alga, Cyanidioschyzon merolae, probed by time-resolved fluorescence spectroscopy

  Yoshifumi Ueno, Shimpei Aikawa, Akihiko Kondo, Seiji Akimoto

  Aug. 2015, PHOTOSYNTHESIS RESEARCH, 125(1-2) (1-2), 211 - 218, English

  [Refereed]

  Scientific journal


• Energy transfer in Anabaena variabilis filaments under nitrogen depletion, studied by time-resolved fluorescence

  Aya Onishi, Shimpei Aikawa, Akihiko Kondo, Seiji Akimoto

  Aug. 2015, PHOTOSYNTHESIS RESEARCH, 125(1-2) (1-2), 191 - 199, English

  [Refereed]

  Scientific journal


• Differences in energy transfer of a cyanobacterium, Synechococcus sp PCC 7002, grown in different cultivation media

  Kenta Niki, Shimpei Aikawa, Makio Yokono, Akihiko Kondo, Seiji Akimoto

  Aug. 2015, PHOTOSYNTHESIS RESEARCH, 125(1-2) (1-2), 201 - 210, English

  [Refereed]

  Scientific journal


• Changes in primary metabolism under light and dark conditions in response to overproduction of a response regulator RpaA in the unicellular cyanobacterium Synechocystis sp PCC 6803

  Hiroko Iijima, Tomokazu Shirai, Mami Okamoto, Akihiko Kondo, Masami Yokota Hirai, Takashi Osanai

  Aug. 2015, FRONTIERS IN MICROBIOLOGY, 6, 888, English

  [Refereed]

  Scientific journal


• Effect of sitagliptin on intrahepatic lipid content and body fat in patients with type 2 diabetes

  Hiroyuki Kato, Yoshio Nagai, Akio Ohta, Ayumi Tenjin, Yuta Nakamura, Hidekazu Tsukiyama, Yosuke Sasaki, Hisashi Fukuda, Toshihiko Ohshige, Yuko Terashima, Yukiyoshi Sada, Akihiko Kondo, Toshiyasu Sasaoka, Yasushi Tanaka

  Elsevier Ireland Ltd, Jul. 2015, Diabetes Research and Clinical Practice, 109(1) (1), 199 - 205, English

  [Refereed]

  Scientific journal


• Production of D-lactic acid from hardwood pulp by mechanical milling followed by simultaneous saccharification and fermentation using metabolically engineered Lactobacillus plantarum

  Shinji Hama, Shino Mizuno, Maki Kihara, Tsutomu Tanaka, Chiaki Ogino, Hideo Noda, Akihiko Kondo

  Jul. 2015, BIORESOURCE TECHNOLOGY, 187, 167 - 172, English

  [Refereed]

  Scientific journal


• Alteration of cyanobacterial sugar and amino acid metabolism by overexpression hik8, encoding a KaiC-associated histidine kinase

  Takashi Osanai, Tomokazu Shirai, Hiroko Iijima, Ayuko Kuwahara, Iwane Suzuki, Akihiko Kondo, Masami Yokota Hirai

  Jul. 2015, ENVIRONMENTAL MICROBIOLOGY, 17(7) (7), 2430 - 2440, English

  [Refereed]

  Scientific journal


• Changes in Lignin and Polysaccharide Components in 13 Cultivars of Rice Straw following Dilute Acid Pretreatment as Studied by Solution-State 2D H-1-C-13 NMR

  Hiroshi Teramura, Kengo Sasaki, Tomoko Oshima, Shimpei Aikawa, Fumio Matsuda, Mami Okamoto, Tomokazu Shirai, Hideo Kawaguchi, Chiaki Ogino, Masanori Yamasaki, Jun Kikuchi, Akihiko Kondo

  Jun. 2015, PLOS ONE, 10(6) (6), e0128417, English

  [Refereed]

  Scientific journal


• Repeated ethanol production from sweet sorghum juice concentrated by membrane separation

  Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

  Jun. 2015, BIORESOURCE TECHNOLOGY, 186, 351 - 355, English

  [Refereed]

  Scientific journal


• Precipitate obtained following membrane separation of hydrothermally pretreated rice straw liquid revealed by 2D NMR to have high lignin content

  Kengo Sasaki, Mami Okamoto, Tomokazu Shirai, Yota Tsuge, Hiroshi Teramura, Daisuke Sasaki, Hideo Kawaguchi, Tomohisa Hasunuma, Chiaki Ogino, Fumio Matsuda, Jun Kikuchi, Akihiko Kondo

  Jun. 2015, BIOTECHNOLOGY FOR BIOFUELS, 8, 88, English

  [Refereed]

  Scientific journal


• Mechanical milling and membrane separation for increased ethanol production during simultaneous saccharification and co-fermentation of rice straw by xylose-fermenting Saccharomyces cerevisiae

  Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Hiroshi Teramura, Kentaro Inokuma, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Jun. 2015, BIORESOURCE TECHNOLOGY, 185, 263 - 268, English

  [Refereed]

  Scientific journal


• 新たな放射線増感剤としての過酸化チタンナノ粒子は膵癌治療における抗腫瘍効果を増強させる(Titanium Peroxide Nanoparticles, as Novel Radiosensitizers, Enhance Antitumor Efficacy in Pancreatic Cancer Therapy)

  Nakayama Masao, Sasaki Ryohei, Ogino Chiaki, Tanaka Tsutomu, Morita Kenta, Umetsu Mitsuo, Ohara Satoshi, Sato Kazuyoshi, Numako Chiya, Takami Seiichi, Kondo Akihiko

  (一社)日本放射線影響学会, May 2015, 日本放射線影響学会大会講演要旨集, 58回, 3 - 07, English


• Evaluation of Brachypodium distachyon L-Tyrosine Decarboxylase Using L-Tyrosine Over-Producing Saccharomyces cerevisiae

  Shuhei Noda, Tomokazu Shirai, Keiichi Mochida, Fumio Matsuda, Sachiko Oyama, Mami Okamoto, Akihiko Kondo

  May 2015, PLOS ONE, 10(5) (5), e0125488, English

  [Refereed]

  Scientific journal


• CBPによるバイオエタノール生産技術の開発

  INOKUMA KENTARO, HASUNUMA TOMOHISA, KONDO AKIHIKO

  シーエムシー出版, May 2015, バイオインダストリー, 32(5) (5), 26 - 31, Japanese

  Scientific journal


• Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse

  Hideo Kawaguchi, Hiroshi Teramura, Kouji Uematsu, Kiyotaka Y. Hara, Tomohisa Hasunuma, Ko Hirano, Takashi Sazuka, Hidemi Kitano, Yota Tsuge, Prihardi Kahar, Satoko Niimi-Nakamura, Ken-Ichi Oinuma, Naoki Takaya, Shigemitsu Kasuga, Chiaki Ogino, Akihiko Kondo

  Apr. 2015, BIORESOURCE TECHNOLOGY, 182, 169 - 178, English

  [Refereed]

  Scientific journal


• Improving polyglucan production in cyanobacteria and microalgae via cultivation design and metabolic engineering

  Akihiko Kondo

  Apr. 2015, Biotechnology Journal, 10(6) (6), 886 - 898, English

  [Refereed]

  Scientific journal


• Improved production of phospholipase A(1) by recombinant Aspergillus oryzae through immobilization to control the fungal morphology under nutrient-limited conditions

  Shinji Hama, Kaoru Onodera, Ayumi Yoshida, Hideo Noda, Akihiko Kondo

  Apr. 2015, BIOCHEMICAL ENGINEERING JOURNAL, 96, 1 - 6, English

  [Refereed]

  Scientific journal


• Eliminating the isoleucine biosynthetic pathway to reduce competitive carbon outflow during isobutanol production by Saccharomyces cerevisiae

  Kengo Ida, Jun Ishii, Fumio Matsuda, Takashi Kondo, Akihiko Kondo

  Apr. 2015, MICROBIAL CELL FACTORIES, 14, 62, English

  [Refereed]

  Scientific journal


• Evaluation of genes involved in oxidative phosphorylation in yeast by developing a simple and rapid method to measure mitochondrial ATP synthetic activity

  Xiaoting Ye, Kana Morikawa, Shih-Hsin Ho, Michihiro Araki, Keiji Nishida, Tomohisa Hasunuma, Kiyotaka Y. Hara, Akihiko Kondo

  Apr. 2015, Microbial Cell Factories, 14, 56, English

  [Refereed]

  Scientific journal


• Recent advances in the metabolic engineering of Corynebacterium glutamicum for the production of lactate and succinate from renewable resources

  Yota Tsuge, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2015, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 42(3) (3), 375 - 389, English

  [Refereed]

  Scientific journal


• M-path: a compass for navigating potential metabolic pathways

  Michihiro Araki, Robert Sidney Cox, Hiroki Makiguchi, Teppei Ogawa, Takeshi Taniguchi, Kohei Miyaoku, Masahiko Nakatsui, Kiyotaka Y. Hara, Akihiko Kondo

  Mar. 2015, BIOINFORMATICS, 31(6) (6), 905 - 911, English

  [Refereed]

  Scientific journal


• Improved ethanol production from xylose in the presence of acetic acid by the overexpression of the HAA1 gene in Saccharomyces cerevisiae

  Yuri Sakihama, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2015, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 119(3) (3), 297 - 302, English

  [Refereed]

  Scientific journal


• Dynamic metabolic profiling of the marine microalga Chlamydomonas sp JSC4 and enhancing its oil production by optimizing light intensity

  Shih-Hsin Ho, Akihito Nakanishi, Xiaoting Ye, Jo-Shu Chang, Chun-Yen Chen, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2015, BIOTECHNOLOGY FOR BIOFUELS, 8, 48, English

  [Refereed]

  Scientific journal


• 4-Vinylphenol biosynthesis from cellulose as the sole carbon source using phenolic acid decarboxylase- and tyrosine ammonia lyase-expressing Streptomyces lividans

  Shuhei Noda, Yoshifumi Kawai, Tsutomu Tanaka, Akihiko Kondo

  Mar. 2015, BIORESOURCE TECHNOLOGY, 180, 59 - 65, English

  [Refereed]

  Scientific journal


• Polyamino acid display on cell surfaces enhances salt and alcohol tolerance of Escherichia coli

  Hirokazu Suzuki, Jun Ishii, Akihiko Kondo, Ken-ichi Yoshida

  Feb. 2015, BIOTECHNOLOGY LETTERS, 37(2) (2), 429 - 435, English

  [Refereed]

  Scientific journal


• Mutation of arginine residues to avoid non-specific cellular uptakes for hepatitis B virus core particles

  Izzat Fahimuddin Bin Mohamed Suffian, Yuya Nishimura, Kenta Morita, Sachiko Nakamura-Tsuruta, Khuloud T. Al-Jamal, Jun Ishii, Chiaki Ogino, Akihiko Kondo

  Feb. 2015, JOURNAL OF NANOBIOTECHNOLOGY, 13(1) (1), 15, English

  [Refereed]

  Scientific journal


• Improving the odorant sensitivity of olfactory receptor-expressing yeast with accessory proteins

  Yosuke Fukutani, Ayaka Hori, Satoshi Tsukada, Ryoichi Sato, Jun Ishii, Akihiko Kondo, Hiroaki Matsunami, Masafumi Yohda

  Feb. 2015, ANALYTICAL BIOCHEMISTRY, 471, 1 - 8, English

  [Refereed]

  Scientific journal


• Efficient co-displaying and artificial ratio control of alpha-amylase and glucoamylase on the yeast cell surface by using combinations of different anchoring domains

  Kentaro Inokuma, Takanobu Yoshida, Jun Ishii, Tomohisa Hasunuma, Akihiko Kondo

  Feb. 2015, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 99(4) (4), 1655 - 1663, English

  [Refereed]

  Scientific journal


• Cell-surface display of enzymes by the yeast Saccharomyces cerevisiae for synthetic biology

  Akihiko Kondo

  Feb. 2015, FEMS Yeast Research, 15, 1 - 9, English

  [Refereed]

  Scientific journal


• Intra-Arterial Transplantation of Allogeneic Mesenchymal Stem Cells Mounts Neuroprotective Effects in a Transient Ischemic Stroke Model in Rats: Analyses of Therapeutic Time Window and Its Mechanisms

  Akihiko Kondo

  2015, Plos One

  Scientific journal


• Maternal osteocalcin practice permits pups to escape from their prospective metabolic syndrome

  Akihiko Kondo

  2015, Journal of Pharmacological Sciences

  Scientific journal


• Land-Cover Change and Climate Change Analysis of the Amur River Basin Using Remote Sensing Data

  Akihiko Kondo

  2015, Environmental Change and the Social Response in the Amur River Basin

  Scientific journal


• Improving the odorant sensitivity of olfactory receptor-expressing yeast with accessory proteins

  Akihiko Kondo

  2015, Analytical Biochemistry

  Scientific journal


• Nanoparticle synthesis by biogenic approach

  Sarvesh Kumar Srivastava, Chiaki Ogino, Akihiko Kondo

  Springer International Publishing, Jan. 2015, Green Processes for Nanotechnology: From Inorganic to Bioinspired Nanomaterials, 237 - 257, English

  [Refereed]

  In book


• 代謝工学を利用したCorynebacterium glutamicumによる乳酸およびコハク酸の生産

  KONDO AKIHIKO, 柘植 洋太

  バイオインダストリー協会, 2015, バイオサイエンスとインダストリー, 73(5) (5), 362 - 368, Japanese

  Scientific journal


• 合成生物工学の未来展望

  KONDO AKIHIKO, 植田 充美

  2015, 生物工学会誌, 93(9) (9), 522, Japanese

  Scientific journal


• Green Processes for Nanotechnology, Chapter 8, Nanoparticle Synthesis

  Srivastave S.K, Ogino Chiaki, KONDO AKIHIKO

  2015, Biogenic Approach, Springer, 237 - 257, Japanese

  Scientific journal


• Effective saccharification of kraft pulp by using a cellulase cocktail prepared from genetically engineered Aspergillus oryzae

  Ryosuke Yamada, Toshihide Yoshie, Shoji Sakai, Satoshi Wakai, Nanami Asai-Nakashima, Fumiyoshi Okazaki, Chiaki Ogino, Hiromoto Hisada, Hiroko Tsutsumi, Yoji Hata, Akihiko Kondo

  Japan Society for Bioscience Biotechnology and Agrochemistry, 2015, Bioscience, Biotechnology and Biochemistry, 79(6) (6), 1034 - 1037, English

  [Refereed]

  Scientific journal


• Secretory production of tetrameric native full-length streptavidin with thermostability using Streptomyces lividans as a host

  NODA S, MATSUMOTO Takuya, TANAKA Tsutomu, KONDO Akihiko

  BACKGROUND: Streptavidin is a tetrameric protein derived from Streptomyces avidinii, and has tight and specific biotin binding affinity. Applications of the streptavidin-biotin system have been widely studied. Streptavidin is generally produced using protein expression in Escherichia coli. In the present study, the secretory production of streptavidin was carried out using Streptomyces lividans as a host. RESULTS: In this study, we used the gene encoding native full-length streptavidin, whereas the core region is generally used for streptavidin production in E. coli. Tetrameric streptavidin composed of native full-length streptavidin monomers was successfully secreted in the culture supernatant of S. lividans transformants, and had specific biotin binding affinity as strong as streptavidin produced by E. coli. The amount of Sav using S. lividans was about 9 times higher than using E. coli. Surprisingly, streptavidin produced by S. lividans exhibited affinity to biotin after boiling, despite the fact that tetrameric streptavidin is known to lose its biotin binding ability after brief boiling. CONCLUSION: We successfully produced a large amount of tetrameric streptavidin as a secretory-form protein with unique thermotolerance.

  Jan. 2015, Microbial Cell Factories, 14(1) (1), 5 - 5, English, International magazine

  [Refereed]

  Scientific journal


• Multi-functional glycoside hydrolase: Blon_0625 from Bifidobacterium longum subsp infantis ATCC 15697

  Takuya Matsumoto, Shota Shimada, Yuto Hata, Tsutomu Tanaka, Akihiko Kondo

  Jan. 2015, ENZYME AND MICROBIAL TECHNOLOGY, 68, 10 - 14, English

  [Refereed]

  Scientific journal


• Expression of cold-adapted beta-1,3-xylanase as a fusion protein with a ProS2 tag and purification using immobilized metal affinity chromatography with a high concentration of ArgHCl

  Motonori Kudou, Fumiyoshi Okazaki, Nanami Asai-Nakashima, Chiaki Ogino, Akihiko Kondo

  Jan. 2015, BIOTECHNOLOGY LETTERS, 37(1) (1), 89 - 94, English

  [Refereed]

  Scientific journal


• The impact of zinc sulfate addition on the dynamic metabolic profiling of Saccharomyces cerevisiae subjected to long term acetic acid stress treatment and identification of key metabolites involved in the antioxidant effect of zinc

  Chun Wan, Mingming Zhang, Qing Fang, Liang Xiong, Xinqing Zhao, Tomohisa Hasunuma, Fengwu Bai, Akihiko Kondo

  2015, METALLOMICS, 7(2) (2), 322 - 332, English

  [Refereed]

  Scientific journal


• The mapping of yeast's G-protein coupled receptor with an atomic force microscope

  Musashi Takenaka, Yusuke Miyachi, Jun Ishii, Chiaki Ogino, Akihiko Kondo

  2015, NANOSCALE, 7(11) (11), 4956 - 4963, English

  [Refereed]

  Scientific journal


• Zinc, magnesium, and calcium ion supplementation confers tolerance to acetic acid stress in industrial Saccharomyces cerevisiae utilizing xylose

  Ku Syahidah Ku Ismail, Takatoshi Sakamoto, Tomohisa Hasunuma, Xin-Qing Zhao, Akihiko Kondo

  Dec. 2014, BIOTECHNOLOGY JOURNAL, 9(12) (12), 1519 - 1525, English

  [Refereed]

  Scientific journal


• Perspectives on engineering strategies for improving biofuel production from microalgae - A critical review

  Shih-Hsin Ho, Xiaoting Ye, Tomohisa Hasunuma, Jo-Shu Chang, Akihiko Kondo

  Dec. 2014, BIOTECHNOLOGY ADVANCES, 32(8) (8), 1448 - 1459, English

  [Refereed]

  Scientific journal


• Overexpression of flv3 improves photosynthesis in the cyanobacterium Synechocystis sp PCC6803 by enhancement of alternative electron flow

  Tomohisa Hasunuma, Mami Matsuda, Youhei Senga, Shimpei Aikawa, Masakazu Toyoshima, Ginga Shimakawa, Chikahiro Miyake, Akihiko Kondo

  Dec. 2014, BIOTECHNOLOGY FOR BIOFUELS, 7, 493, English

  [Refereed]

  Scientific journal


• L-lactic acid production from starch by simultaneous saccharification and fermentation in a genetically engineered Aspergillus oryzae pure culture

  Satoshi Wakai, Toshihide Yoshie, Nanami Asai-Nakashima, Ryosuke Yamada, Chiaki Ogino, Hiroko Tsutsumi, Yoji Hata, Akihiko Kondo

  Dec. 2014, BIORESOURCE TECHNOLOGY, 173, 376 - 383, English

  [Refereed]

  Scientific journal


• Development of bio-based fine chemical production through synthetic bioengineering

  Kiyotaka Y. Hara, Michihiro Araki, Naoko Okai, Satoshi Wakai, Tomohisa Hasunuma, Akihiko Kondo

  Dec. 2014, MICROBIAL CELL FACTORIES, 13(1) (1), 173, English

  [Refereed]

  Scientific journal


• Development of a multi-gene expression system in Xanthophyllomyces dendrorhous

  Kiyotaka Y. Hara, Toshihiko Morita, Masao Mochizuki, Keisuke Yamamoto, Chiaki Ogino, Michihiro Araki, Akihiko Kondo

  Dec. 2014, MICROBIAL CELL FACTORIES, 13(1) (1), 175, English

  [Refereed]

  Scientific journal


• Sequence diversity and gene expression analyses of expansin-related proteins in the white-rot basidiomycete, Phanerochaete carnosa

  Hitoshi Suzuki, Thu V. Vuong, Yunchen Gong, Kin Chan, Chi-Yip Ho, Emma R. Master, Akihiko Kondo

  Nov. 2014, FUNGAL GENETICS AND BIOLOGY, 72, 115 - 123, English

  [Refereed]

  Scientific journal


• Construction of a Yeast-Based Signaling Biosensor for Human Angiotensin II Type 1 Receptor via Functional Coupling Between Asn295-Mutated Receptor and Gpa1/G(i3) Chimeric G alpha

  Yasuyuki Nakamura, Jun Ishii, Akihiko Kondo

  Nov. 2014, BIOTECHNOLOGY AND BIOENGINEERING, 111(11) (11), 2220 - 2228, English

  [Refereed]

  Scientific journal


• Comparison of metabolomic profiles of microbial communities between stable and deteriorated methanogenic processes

  Daisuke Sasaki, Kengo Sasaki, Yota Tsuge, Masahiko Morita, Akihiko Kondo

  Nov. 2014, BIORESOURCE TECHNOLOGY, 172, 83 - 90, English

  [Refereed]

  Scientific journal


• Cloning and starch degradation profile of maltotriose-producing amylases from Streptomyces species

  Norimasa Kashiwagi, Michiru Miyake, Shuichi Hirose, Masahiro Sota, Chiaki Ogino, Akihiko Kondo

  Nov. 2014, BIOTECHNOLOGY LETTERS, 36(11) (11), 2311 - 2317, English

  [Refereed]

  Scientific journal


• Optimized membrane process to increase hemicellulosic ethanol production from pretreated rice straw by recombinant xylose-fermenting Saccharomyces cerevisiae

  Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Tomohisa Hasunuma, Takatoshi Sakamoto, Yuri Sakihama, Chiaki Ogino, Akihiko Kondo

  Oct. 2014, BIORESOURCE TECHNOLOGY, 169, 380 - 386, English

  [Refereed]

  Scientific journal


• Increased ethanol production from sweet sorghum juice concentrated by a membrane separation process

  Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Hiroshi Teramura, Satoshi Wakai, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo

  Oct. 2014, BIORESOURCE TECHNOLOGY, 169, 821 - 825, English

  [Refereed]

  Scientific journal


• Development of a GIN11/FRT-based multiple-gene integration technique affording inhibitor-tolerant, hemicellulolytic, xylose-utilizing abilities to industrial Saccharomyces cerevisiae strains for ethanol production from undetoxified lignocellulosic hemicelluloses

  Tomohisa Hasunuma, Yoshimi Hori, Takatoshi Sakamoto, Misa Ochiai, Haruyo Hatanaka, Akihiko Kondo

  Oct. 2014, MICROBIAL CELL FACTORIES, 13(1) (1), 145, English

  [Refereed]

  Scientific journal


• Detoxification of furfural in Corynebacterium glutamicum under aerobic and anaerobic conditions

  Yota Tsuge, Yoshimi Hori, Motonori Kudou, Jun Ishii, Tomohisa Hasunuma, Akihiko Kondo

  Oct. 2014, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98(20) (20), 8675 - 8683, English

  [Refereed]

  Scientific journal


• A new screening method for recombinant Saccharomyces cerevisiae strains based on their xylose fermentation ability measured by near infrared spectroscopy

  Hiroyuki Morita, Tomohisa Hasunuma, Maria Vassileva, Akihiko Kondo, Roumiana Tsenkova

  Sep. 2014, ANALYTICAL METHODS, 6(17) (17), 6628 - 6634, English

  [Refereed]

  Scientific journal


• Genetic Engineering of Bio-Nanoparticles for Drug Delivery: A Review

  Yuya Nishimura, Jun Ishii, Chiaki Ogino, Akihiko Kondo

  Sep. 2014, JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 10(9) (9), 2063 - 2085, English

  [Refereed]

  Scientific journal


• Desired Alteration of Protein Affinities: Competitive Selection of Protein Variants Using Yeast Signal Transduction Machinery

  Misato Kaishima, Nobuo Fukuda, Jun Ishii, Akihiko Kondo

  Sep. 2014, PLOS ONE, 9(9) (9), e108229, English

  [Refereed]

  Scientific journal


• 大腸菌のパスウェイエンジニアリングによる安価な基質からのパクリタキセル前駆体タキサジエンの効率的生産

  竹村 秀史, 鈴木 宗典, 梅野 太輔, 原田 尚志, 三沢 典彦, 石井 純, 近藤 昭彦, 播本 孝史

  (公社)日本生物工学会, Aug. 2014, 日本生物工学会大会講演要旨集, 平成26年度, 161 - 161, Japanese


• Signaling assays for detection of human G-protein-coupled receptors in yeast

  Yasuyuki Nakamura, Jun Ishii, Akihiko Kondo

  Aug. 2014, Bio-protocol, 4(16) (16), e1206, English

  [Refereed]

  Scientific journal


• Short-term light adaptation of a cyanobacterium, Synechocystis sp. PCC 6803, probed by time-resolved fluorescence spectroscopy

  Seiji Akimoto, Makio Yokono, Erina Yokono, Shimpei Aikawa, Akihiko Kondo

  Aug. 2014, PLANT PHYSIOLOGY AND BIOCHEMISTRY, 81, 149 - 154, English

  [Refereed]

  Scientific journal


• Pretreatment of Japanese cedar by ionic liquid solutions in combination with acid and metal ion and its application to high solid loading

  Kazuma Ogura, Kazuaki Ninomiya, Kenji Takahashi, Chiaki Ogino, Akihiko Kondo

  Aug. 2014, BIOTECHNOLOGY FOR BIOFUELS, 7, 120, English

  [Refereed]

  Scientific journal


• Evaluation and screening of efficient promoters to improve astaxanthin production in Xanthophyllomyces dendrorhous

  Kiyotaka Y. Hara, Toshihiko Morita, Yusuke Endo, Masao Mochizuki, Michihiro Araki, Akihiko Kondo

  Aug. 2014, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98(15) (15), 6787 - 6793, English

  [Refereed]

  Scientific journal


• アーミング酵母による統合型バイオプロセスの開発

  HASUNUMA TOMOHISA, KONDO AKIHIKO

  To build an energy and material secure future, a next generation of renewable fuels and chemicals produced from lignocellulosic biomass is required. Since lignocellulosic biomass represents an abundant carbonneutral renewable resource for the production of biofuels, numerous environmental and social benefits could result from the replacement of petroleum-based transport fuels with bioethanol. However, the complicated ethanol production processes involved make the cost of producing bioethanol from it higher compared to corn starch and cane juice. Therefore, consolidated bioprocessing (CBP), which combines enzyme production, saccharification and fermentation in a single step, has gained increased recognition as a potential bioethanol production system. CBP requires a highly engineered microorganism developed for several different processspecific characteristics. The dominant strategy for engineering a CBP biocatalyst is to express multiple components of a cellulolytic system from either fungi or bacteria in the yeast Saccharomyces cerevisiae. The development of recombinant yeast strains displaying cellulases and hemicellulases on the cell surface, which are referred to as arming yeast strains, represents significant progress toward realization of CBP. The engineered yeast strains successfully perform the direct ethanol production from lignocellulosic biomass. The yeast is reusable for the hydrolysis and fermentation by collecting the cells. Reutilization of the cell-surface engineered yeast has an advantage in the reduction of enzyme cost, which enables reuse of enzymes on the cell surface. On the other hand, the difference in optimum temperature between saccharification and fermentation is a drawback of efficient ethanol production in the simultaneous saccharification and fermentation (SSF). The application of thermotolerant yeast strains engineered to the SSF process would overcome the drawback by performing hydrolysis and fermentation at elevated temperature.

  The Japan Institute of Energy, Jul. 2014, 日本エネルギー学会誌, 93(7) (7), 580 - 585, Japanese

  Scientific journal


• The effect of combining signal sequences with the N28 fragment on GFP production in Aspergillus oryzae

  Chiak Ogino, Tetsuya Matsuda, Fumiyoshi Okazaki, Tsutomu Tanaka, Akihiko Kondo

  Jul. 2014, PROCESS BIOCHEMISTRY, 49(7) (7), 1078 - 1083, English

  [Refereed]

  Scientific journal


• Simultaneous saccharification and fermentation of kraft pulp by recombinant Escherichia coli for phenyllactic acid production

  Hideo Kawaguchi, Kouji Uematsu, Chiaki Ogino, Hiroshi Teramura, Satoko Niimi-Nakamura, Yota Tsuge, Tomohisa Hasunuma, Ken-Ichi Oinuma, Naoki Takaya, Akihiko Kondo

  Jul. 2014, BIOCHEMICAL ENGINEERING JOURNAL, 88, 188 - 194, English

  [Refereed]

  Scientific journal


• Creation of Cellobiose and Xylooligosaccharides-Coutilizing Escherichia con Displaying both beta-Glucosidase and beta-Xylosidase on Its Cell Surface

  Tsutomu Tanaka, Yuuki Hirata, Mariko Nakano, Hitomi Kawabata, Akihiko Kondo

  Jul. 2014, ACS SYNTHETIC BIOLOGY, 3(7) (7), 446 - 453, English

  [Refereed]

  Scientific journal


• 酵素触媒法によるバイオディーゼル燃料生産システムのスケールアップ

  濱 真司, 吉田 あゆみ, 小野寺 かおる, 蔵田 兼司, KONDO AKIHIKO, 野田 秀夫

  An enzymatic packed-bed reactor (PBR) integrated with a glycerol-separating system was used for batch methanolysis of waste cooking oil, which was further applied to continuous flow- and circulating reaction systems for scale-up. In a batch reaction system, methyl ester content in the effluent and amount of removed glycerol in the glycerol-separating tank increased with increasing cycle number of the reaction mixture, yielding 94% and 104g (based on 1000g of waste cooking oil), respectively. The PBR was successfully operated over 30 batches realizing both a high methyl ester content and an efficient glycerol removal. In a continuous flow reaction system, where ten PBRs were connected in series, 5.4L/h of the effluent with a methyl ester content of 95.7% was obtained from waste cooking oil with the reduced viscosity. After glycerol adsorption and methanol evaporation, the resulting product (B5) met the standards specified in the act on the quality control of gasoline and other fuels. In a circulating reaction system, where the effluent is continuously fed into the material tank, reaction rate of the PBR was lower than that in a flow system; however, the results show that the circulating reaction unit possessing one PBR is applicable to the production of biodiesel at 200L/day.

  日本生物工学会, Jun. 2014, 生物工学会誌, 92(6) (6), 262 - 269, Japanese

  Scientific journal


• 「バイオプロダクション次世代農工連携研究」と拠点形成への展望

  KONDO AKIHIKO, OGINO CHIAKI

  テックタイムス, Jun. 2014, 紙パルプ技術タイムス, 57(6) (6), 51 - 56, Japanese

  Scientific journal


• Two-step production of D-lactate from mixed sugars by growing and resting cells of metabolically engineered Lactobacillus plantarum.

  Yota Tsuge, Hideo Kawaguchi, Kengo Sasaki, Tsutomu Tanaka, Akihiko Kondo

  To develop cost-effective systems for D-lactate production, here, the effect of high-cell density cultivation of metabolically engineered Lactobacillus plantarum on D-lactate production was evaluated. A xylose-assimilating strain of L. plantarum was anaerobically cultured with mixed sugars (glucose and xylose) as substrates. Compared to undiluted nutrient-rich de Man, Rogosa, and Sharpe (MRS) medium, D-lactate production by cultivating in 10-fold diluted MRS (0.1 MRS) medium or normal saline solution was 89.7 and 81.3 %, respectively. Notably, the xylose consumption rate was comparable in the three cultures, whereas the glucose consumption rate decreased by 18.3 and 26.1 % in 0.1 MRS medium and normal saline solution, respectively, resulting in a reduction of the D-lactate production rate. The D-lactate productivity in high-cell density cultivation was proportional to the initial cell concentrations. The use of a two-step cultivation process involving growing and resting cells in a single bioreactor revealed that the ratio of the glucose and xylose consumption rates (based on grams consumed) in resting cell conditions was 1.88, whereas that in growing conditions was 2.58. Cultivation of L. plantarum in growing conditions for 24 h produced 73.2 g/l D-lactate with the yield of 0.90 g/g, whereas cells cultivation under resting cell conditions in a saline solution for 24 h produced 68.7 g/l D-lactate with the yield of 0.93 g/g. In total, 141.9 g/l D-lactate was produced after 48 h cultivation, a value that represents the highest reported concentration of D-lactate produced from mixed sugars to date. Our findings contribute to the cost-effective, large-scale production of D-lactate.

  Jun. 2014, Applied microbiology and biotechnology, 98(11) (11), 4911 - 8, English, International magazine

  [Refereed]

  Scientific journal


• Optimizing biodiesel production in marine Chlamydomonas sp JSC4 through metabolic profiling and an innovative salinity-gradient strategy

  Shih-Hsin Ho, Akihito Nakanishi, Xiaoting Ye, Jo-Shu Chang, Kiyotaka Hara, Tomohisa Hasunuma, Akihiko Kondo

  Jun. 2014, BIOTECHNOLOGY FOR BIOFUELS, 7, 97, English

  [Refereed]

  Scientific journal


• Improvement of enzymatic activity of beta-glucosidase from Thermotoga maritima by 1-butyl-3-methylimidazolium acetate

  Motonori Kudou, Yuka Kubota, Nanami Nakashima, Fumiyoshi Okazaki, Kazunori Nakashima, Chiaki Ogino, Akihiko Kondo

  Jun. 2014, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 104, 17 - 22, English

  [Refereed]

  Scientific journal


• Glycogen production for biofuels by the euryhaline cyanobacteria Synechococcus sp strain PCC 7002 from an oceanic environment

  Shimpei Aikawa, Atsumi Nishida, Shih-Hsin Ho, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo

  Jun. 2014, BIOTECHNOLOGY FOR BIOFUELS, 7, 88, English

  [Refereed]

  Scientific journal


• Three gene expression vector sets for concurrently expressing multiple genes in Saccharomyces cerevisiae

  Jun Ishii, Takashi Kondo, Harumi Makino, Akira Ogura, Fumio Matsuda, Akihiko Kondo

  May 2014, FEMS YEAST RESEARCH, 14(3) (3), 399 - 411, English

  [Refereed]

  Scientific journal


• Increase in lactate yield by growing Corynebacterium glutamicum in a bioelectrochemical reactor

  Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Akihiko Kondo

  May 2014, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 117(5) (5), 598 - 601, English

  [Refereed]

  Scientific journal


• Aspergillus oryzae-based cell factory for direct kojic acid production from cellulose

  Ryosuke Yamada, Toshihide Yoshie, Satoshi Wakai, Nanami Asai-Nakashima, Fumiyoshi Okazaki, Chiaki Ogino, Hiromoto Hisada, Hiroko Tsutsumi, Yoji Hata, Akihiko Kondo

  May 2014, MICROBIAL CELL FACTORIES, 13, 71, English

  [Refereed]

  Scientific journal


• Lactic Acid

  Kenji Okano, Tsutomu Tanaka, Akihiko Kondo

  Wiley Blackwell, Apr. 2014, Bioprocessing of Renewable Resources to Commodity Bioproducts, 353 - 380, English

  [Refereed]

  In book


• Ethanol Production from Yeasts

  Tomohisa Hasunuma, Ryosuke Yamada, Akihiko Kondo

  Wiley Blackwell, Apr. 2014, Bioprocessing of Renewable Resources to Commodity Bioproducts, 201 - 226, English

  [Refereed]

  In book


• 植物の機能解明と新たな機能材料創出の可能性

  寺村 浩, 延田 紘治, KAWAGUCHI HIDEO, OGINO CHIAKI, NISHINO TAKASHI, KONDO AKIHIKO

  シーエムシー出版, Apr. 2014, バイオインダストリー, 31(4) (4), 62 - 68, Japanese

  Scientific journal


• Saccharification behavior of cellulose acetate during enzymatic processing for microbial ethanol production

  Shinji Hama, Kohsuke Nakano, Kaoru Onodera, Masashi Nakamura, Hideo Noda, Akihiko Kondo

  Apr. 2014, BIORESOURCE TECHNOLOGY, 157, 1 - 5, English

  [Refereed]

  Scientific journal


• Disruption of pknG enhances production of gamma-aminobutyric acid by Corynebacterium glutamicum expressing glutamate decarboxylase

  Naoko Okai, Chihiro Takahashi, Kazuki Hatada, Chiaki Ogino, Akihiko Kondo

  Apr. 2014, AMB EXPRESS, 4, 20, English

  [Refereed]

  Scientific journal


• A display of pH-sensitive fusogenic GALA peptide facilitates endosomal escape from a Bio-nanocapsule via an endocytic uptake pathway

  Yuya Nishimura, Koichi Takeda, Ryosuke Ezawa, Jun Ishii, Chiaki Ogino, Akihiko Kondo

  Apr. 2014, JOURNAL OF NANOBIOTECHNOLOGY, 12(1) (1), 11, English

  [Refereed]

  Scientific journal


• リグノセルロース系バイオマスからのバイオ燃料・バイオ化学品生産プロセスの開発

  KONDO AKIHIKO

  The Society of Fiber Science and Technology, Japan, Mar. 2014, 繊維と工業, 70(3) (3), 99 - 102, Japanese

  Scientific journal


• リグノセルロースからの高効率エタノール生産に向けた新規代謝改変酵母の創製

  HASUNUMA TOMOHISA, KONDO AKIHIKO

  To build an energy and material secure future, a next generation of renewable fuels and bio-based chemicals produced from lignocellulosic biomass is required. Consolidated bioprocessing (CBP), which integrates enzyme production, saccharification and fermentation into a single process, is a promising strategy for effective production of bio-based materials. As a key technology for the development of recombinant cellulolytic microbial strains, cell surface engineering, which enables the display through genetic engineering of various types of functional proteins on microbial cell surfaces without loss of their function, is a promising tool reducing the requirement for cellulase addition, as cellulases can be displayed on the microbial cell surface. Regardless of the process used for the biomass hydrolysis, CBP-enabling microorganisms encounter a variety of toxic compounds released during biomass pretreatment that inhibit microbial growth and ethanol yield. Systems biology approaches including transcriptomics and metabolomics have been recently exploited to gain insight into the molecular and genetic traits involved in tolerance and adaptation to the fermentation inhibitors. A combination of a cell surface displayed enzyme system and an intracellular metabolic engineering system is a very effective approach to develop cells with novel fermentation ability for industrial applications.

  Japan Oil Chemists' Society, Mar. 2014, オレオサイエンス, 14(3) (3), 95 - 101, Japanese

  Scientific journal


• Simultaneous Method for Analyzing Dimerization and Signaling of G-Protein-Coupled Receptor in Yeast by Dual-Color Reporter System

  Yasuyuki Nakamura, Norika Takemoto, Jun Ishii, Akihiko Kondo

  Mar. 2014, BIOTECHNOLOGY AND BIOENGINEERING, 111(3) (3), 586 - 596, English

  [Refereed]

  Scientific journal


• Increased biomass production and glycogen accumulation in apcE gene deleted Synechocystis sp PCC 6803

  Ancy Joseph, Shimpei Aikawa, Kengo Sasaki, Fumio Matsuda, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2014, AMB EXPRESS, 4, 17, English

  [Refereed]

  Scientific journal


• Structural Evaluation of the DNA Aptamer for ATP DH25.42 by AFM

  Akihiko Kondo

  Feb. 2014, Nucleosides, Nucleotides and Nucleic Acids, 33(1) (1), 31 - 39, English

  [Refereed]

  Scientific journal


• Microbial fluorescence sensing for human neurotensin receptor type 1 using G alpha-engineered yeast cells

  Jun Ishii, Asami Oda, Shota Togawa, Akira Fukao, Toshinobu Fujiwara, Chiaki Ogino, Akihiko Kondo

  Feb. 2014, ANALYTICAL BIOCHEMISTRY, 446, 37 - 43, English

  [Refereed]

  Scientific journal


• High-throughput de novo screening of receptor agonists with an automated single-cell analysis and isolation system

  Nobuo Yoshimoto, Kenji Tatematsu, Masumi Iijima, Tomoaki Niimi, Andres D. Maturana, Ikuo Fujii, Akihiko Kondo, Katsuyuki Tanizawa, Shun'ichi Kuroda

  Feb. 2014, SCIENTIFIC REPORTS, 4, 4242, English

  [Refereed]

  Scientific journal


• Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural

  Tomohisa Hasunuma, Ku Syahidah Ku Ismail, Yumiko Nambu, Akihiko Kondo

  Feb. 2014, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 117(2) (2), 165 - 169, English

  [Refereed]

  Scientific journal


• Cell wall structure suitable for surface display of proteins in Saccharomyces cerevisiae

  Hiroyuki Matsuoka, Kazuya Hashimoto, Aki Saijo, Yuki Takada, Akihiko Kondo, Mitsuyoshi Ueda, Hiroshi Ooshima, Taro Tachibana, Masayuki Azuma

  Feb. 2014, YEAST, 31(2) (2), 67 - 76, English

  [Refereed]

  Scientific journal


• Capillary electrophoresis-mass spectrometry reveals the distribution of carbon metabolites during nitrogen starvation in Synechocystis sp PCC 6803

  Takashi Osanai, Akira Oikawa, Tomokazu Shirai, Ayuko Kuwahara, Hiroko Iijima, Kan Tanaka, Masahiko Ikeuchi, Akihiko Kondo, Kazuki Saito, Masami Yokota Hirai

  Feb. 2014, ENVIRONMENTAL MICROBIOLOGY, 16(2) (2), 512 - 524, English

  [Refereed]

  Scientific journal


• Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp PCC 6803

  Joseph Ancy, Aikawa Shimpei, Sasaki Kengo, Teramura Hiroshi, Hasunuma Tomohisa, Matsuda Fumio, Osanai Takashi, Hirai Masami Yokota, Kondo Akihiko

  Jan. 2014, FEBS LETTERS, 588(3) (3), 466 - 471

  [Refereed]


• Spinal Cord Stimulation Exerts Neuroprotective Effects against Experimental Parkinson's Disease

  Akihiko Kondo

  2014, Plos One

  Scientific journal


• Tungstate nanosheet ink as a photonless and electroless chromic device

  Akihiko Kondo

  2014, Journal of Materials Chemistry C

  Scientific journal


• Importance of smoking and the occurrence of acute poisoning due to waterproof spray

  Akihiko Kondo

  2014, Acute Medicine & Surgery

  Scientific journal


• Cognitive functions in Parkinson's disease: Relation to disease severity and hallucination

  Akihiko Kondo

  2014, Parkinsonism and Related Disorders

  Scientific journal


• Characteristics of very high frequency oscillations of somatosensory evoked potentials in humans with epilepsy

  Akihiko Kondo

  2014, Neurology Asia

  Scientific journal


• Editorial: Asian Congress on Biotechnology 2013

  Akihiko Kondo, Virendra S. Bisaria

  Wiley-VCH Verlag, 2014, Biotechnology Journal, 9(12) (12), 1459 - 1461, English

  [Refereed]

  Scientific journal


• Effect of sitagliptin on glycemic control and beta cell function in japanese patients given basal-supported oral therapy for type 2 diabetes

  Shiko Asai, Akio Ohta, Hiroyuki Kato, Yoshiyuki Sada, Yoshio Nagai, Akihiko Kondo, Toshiyasu Sasaoka, Yasushi Tanaka

  Japan Endocrine Society, 2014, Endocrine Journal, 61(12) (12), 1213 - 1220, English

  [Refereed]

  Scientific journal


• Micro and macro modeling of ground depression due to internal erosion

  Kenichi Maeda, Akihiko Kondo

  American Society of Civil Engineers (ASCE), 2014, Geotechnical Special Publication, (236) (236), 445 - 455, English

  [Refereed]

  International conference proceedings


• Mpath: Computationally-aided design of synthetic metabolic pathways

  Robert Sidney Cox, Masahiko Nakatsuil, Hiroki Makiguchi, Teppei Ogawa, Akihiko Kondo, Michihiro Araki

  Jan. 2014, Synthetic Biology: Engineering, Evolution, and Design Conference 2014, SEED 2014, 54 - 56

  International conference proceedings


• Green synthesis of thiolated graphene nanosheets by alliin (garlic) and its effect on the deposition of gold nanoparticles

  Sarvesh Kumar Srivastava, Chiaki Ogino, Akihiko Kondo

  2014, RSC ADVANCES, 4(12) (12), 5986 - 5989, English

  [Refereed]

  Scientific journal


• Electro-catalytically active Au@Pt nanoparticles for hydrogen evolution reaction: an insight into a tryptophan mediated supramolecular interface towards a universal core-shell synthesis approach

  Sarvesh Kumar Srivastava, Jonathan Sabate del Rio, Ciara K. O'Sullivan, Chiaki Ogino, Akihiko Kondo

  2014, RSC ADVANCES, 4(89) (89), 48458 - 48464, English

  [Refereed]

  Scientific journal


• Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp PCC 6803

  Ancy Joseph, Shimpei Aikawa, Kengo Sasaki, Hiroshi Teramura, Tomohisa Hasunuma, Fumio Matsuda, Takashi Osanai, Masami Yokota Hirai, Akihiko Kondo

  Jan. 2014, FEBS LETTERS, 588(3) (3), 466 - 471, English

  [Refereed]

  Scientific journal


• Efficient yeast cell-surface display of exo- and endo-cellulase using the SED1 anchoring region and its original promoter

  Kentaro Inokuma, Tomohisa Hasunuma, Akihiko Kondo

  Jan. 2014, BIOTECHNOLOGY FOR BIOFUELS, 7(1) (1), 8, English

  [Refereed]

  Scientific journal


• Development of lipid productivities under different CO2 conditions of marine microalgae Chlamydomonas sp JSC4

  Akihito Nakanishi, Shimpei Aikawa, Shih-Hsin Ho, Chun-Yen Chen, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo

  Jan. 2014, BIORESOURCE TECHNOLOGY, 152, 247 - 252, English

  [Refereed]

  Scientific journal


• Respiration accumulates Calvin cycle intermediates for the rapid start of photosynthesis in Synechocystis sp. PCC 6803

  Ginga Shimakawa, Tomohisa Hasunuma, Akihiko Kondo, Mami Matsuda, Amane Makino, Chikahiro Miyake

  Japan Society for Bioscience Biotechnology and Agrochemistry, 2014, Bioscience, Biotechnology and Biochemistry, 78(12) (12), 1997 - 2007, English

  [Refereed]

  Scientific journal


• Direct production of organic acids from starch by cell surface-engineered Corynebacterium glutamicum in anaerobic conditions.

  Yota Tsuge, Toshihiro Tateno, Kengo Sasaki, Tomohisa Hasunuma, Tsutomu Tanaka, Akihiko Kondo

  We produced organic acids, including lactate and succinate, directly from soluble starch under anaerobic conditions using high cell-density cultures of Corynebacterium glutamicum displaying α-amylase (AmyA) from Streptococcus bovis 148 on the cell surface. Notably, reactions performed under anaerobic conditions at 35 and 40°C, which are higher than the optimal growth temperature of 30°C, showed 32% and 19%, respectively, higher productivity of the organic acids lactate, succinate, and acetate compared to that at 30°C. However, α-amylase was not stably anchored and released into the medium from the cell surface during reactions at these higher temperatures, as demonstrated by the 61% and 85% decreases in activity, respectively, from baseline, compared to the only 8% decrease at 30°C. The AmyA-displaying C. glutamicum cells retained their starch-degrading capacity during five 10 h reaction cycles at 30°C, producing 107.8 g/l of total organic acids, including 88.9 g/l lactate and 14.0 g/l succinate. The applicability of cell surface-engineering technology for the production of organic acids from biomass by high cell-density cultures of C. glutamicum under anaerobic conditions was demonstrated.

  Dec. 2013, AMB Express, 3(1) (1), 72 - 72, English, International magazine

  [Refereed]

  Scientific journal


• Two-stage oxidation of glucose by an enzymatic bioanode

  T. Matsumoto, S. Shimada, K. Yamamoto, T. Tanaka, A. Kondo

  Dec. 2013, Fuel Cells, 13(6) (6), 960 - 964, English

  [Refereed]

  Scientific journal


• Thermal Stability and Starch Degradation Profile of alpha-Amylase from Streptomyces avermitilis

  Sang Youn Hwang, Kazunori Nakashima, Naoko Okai, Fumiyoshi Okazaki, Michiru Miyake, Koichi Harazono, Chiaki Ogino, Akihiko Kondo

  Dec. 2013, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 77(12) (12), 2449 - 2453, English

  [Refereed]

  Scientific journal


• Increased isobutanol production in Saccharomyces cerevisiae by eliminating competing pathways and resolving cofactor imbalance

  Fumio Matsuda, Jun Ishii, Takashi Kondo, Kengo Ida, Hironori Tezuka, Akihiko Kondo

  Dec. 2013, MICROBIAL CELL FACTORIES, 12, 119, English

  [Refereed]

  Scientific journal


• Glucose content in the liquid hydrolysate after dilute acid pretreatment is affected by the starch content in rice straw

  Hiroshi Teramura, Tomoko Oshima, Fumio Matsuda, Kengo Sasaki, Chiaki Ogino, Masanori Yamasaki, Akihiko Kondo

  Dec. 2013, BIORESOURCE TECHNOLOGY, 149, 520 - 524, English

  [Refereed]

  Scientific journal


• Bright Fluorescence Monitoring System UtilizingZoanthus sp Green Fluorescent Protein (ZsGreen) for Human G-Protein-Coupled Receptor Signaling in Microbial Yeast Cells

  Yasuyuki Nakamura, Jun Ishii, Akihiko Kondo

  Dec. 2013, PLOS ONE, 8(12) (12), e82237, English

  [Refereed]

  Scientific journal


• An approach for dynamical network reconstruction of simple network motifs

  Masahiko Nakatsui, Michihiro Araki, Akihiko Kondo

  Dec. 2013, BMC SYSTEMS BIOLOGY, 7(suppl 6) (suppl 6), 54, English

  [Refereed]

  Scientific journal


• Modification of energy-transfer processes in the cyanobacterium, Arthrospira platensis, to adapt to light conditions, probed by time-resolved fluorescence spectroscopy

  Seiji Akimoto, Makio Yokono, Shimpei Aikawa, Akihiko Kondo

  Nov. 2013, PHOTOSYNTHESIS RESEARCH, 117(1-3) (1-3), 235 - 243, English

  [Refereed]

  Scientific journal


• Ethanol fermentation by xylose-assimilating Saccharomyces cerevisiae using sugars in a rice straw liquid hydrolysate concentrated by nanofiltration

  Kengo Sasaki, Daisuke Sasaki, Yuri Sakihama, Hiroshi Teramura, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Nov. 2013, BIORESOURCE TECHNOLOGY, 147, 84 - 88, English

  [Refereed]

  Scientific journal


• Endowing non-cellulolytic microorganisms with cellulolytic activity aiming for consolidated bioprocessing

  Ryosuke Yamada, Tomohisa Hasunuma, Akihiko Kondo

  Nov. 2013, BIOTECHNOLOGY ADVANCES, 31(6) (6), 754 - 763, English

  [Refereed]

  Scientific journal


• Differences in excitation energy transfer of Arthrospira platensis cells grown in seawater medium and freshwater medium, probed by time-resolved fluorescence spectroscopy

  Muhammad Arba, Shimpei Aikawa, Kenta Niki, Makio Yokono, Akihiko Kondo, Seiji Akimoto

  Nov. 2013, CHEMICAL PHYSICS LETTERS, 588, 231 - 236, English

  [Refereed]

  Scientific journal


• Hyper secretion of Thermobifida fusca beta-glucosidase via a Tat-dependent signal peptide using Streptomyces lividans

  Takaya Miyazaki, Shuhei Noda, Tsutomu Tanaka, Akihiko Kondo

  Oct. 2013, MICROBIAL CELL FACTORIES, 12, 88, English

  [Refereed]

  Scientific journal


• Real-Time Monitoring of the Transesterification of Soybean Oil and Methanol by Fourier-Transform Infrared Spectroscopy

  Feng Zhang, Daisuke Adachi, Sriappareddy Tamalampudi, Akihiko Kondo, Keisuke Tominaga

  Oct. 2013, ENERGY & FUELS, 27(10) (10), 5957 - 5961, English

  [Refereed]

  Scientific journal


• Time-based comparative transcriptomics in engineered xylose-utilizing Saccharomyces cerevisiae identifies temperature-responsive genes during ethanol production

  Ku Syahidah Ku Ismail, Takatoshi Sakamoto, Tomohisa Hasunuma, Akihiko Kondo

  Sep. 2013, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 40(9) (9), 1039 - 1050, English

  [Refereed]

  Scientific journal


• Engineering strategies for improving the CO2 fixation and carbohydrate productivity of Scenedesmus obliquus CNW-N used for bioethanol fermentation

  Shih-Hsin Ho, Akihiko Kondo, Tomohisa Hasunuma, Jo-Shu Chang

  Sep. 2013, BIORESOURCE TECHNOLOGY, 143, 163 - 171, English

  [Refereed]

  Scientific journal


• Cocktail delta-integration of xylose assimilation genes for efficient ethanol production from xylose in Saccharomyces cerevisiae

  Hiroko Kato, Fumio Matsuda, Ryosuke Yamada, Kento Nagata, Tomokazu Shirai, Tomohisa Hasunuma, Akihiko Kondo

  Sep. 2013, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 116(3) (3), 333 - 336, English

  [Refereed]

  Scientific journal


• A transgene expression system for the marine microalgae Aurantiochytrium sp KRS101 using a mutant allele of the gene encoding ribosomal protein L44 as a selectable transformation marker for cycloheximide resistance

  Won-Kyung Hong, Sun-Yeon Heo, Baek-Rock Oh, Chul Ho Kim, Jung-Hoon Sohn, Ji-Won Yang, Akihiko Kondo, Jeong-Woo Seo

  Sep. 2013, BIOPROCESS AND BIOSYSTEMS ENGINEERING, 36(9) (9), 1191 - 1197, English

  [Refereed]

  Scientific journal


• Direct L-lysine production from cellobiose by Corynebacterium glutamicum displaying beta-glucosidase on its cell surface

  Noriko Adachi, Chihiro Takahashi, Naoko Ono-Murota, Rie Yamaguchi, Tsutomu Tanaka, Akihiko Kondo

  Aug. 2013, Applied Microbiology and Biotechnology, 97(16) (16), 7165 - 7172, English

  [Refereed]

  Scientific journal


• Ectoine production from lignocellulosic biomass-derived sugars by engineered Halomonas elongata

  Kosuke Tanimura, Hideki Nakayama, Tsutomu Tanaka, Akihiko Kondo

  Aug. 2013, BIORESOURCE TECHNOLOGY, 142, 523 - 529, English

  [Refereed]

  Scientific journal


• ナノ粒子とX線照射を併用した深部ガンの非侵襲的治療法の開発

  田野 亜都里, 森田 健太, 荻野 千秋, 佐藤 和好, 沼子 千弥, 中山 雅央, Sasaki Ryohei, 近藤 昭彦

  日本生物工学会, Aug. 2013, 日本生物工学会大会講演要旨集平成25年度, 65, 244 - 244, Japanese

  Research society


• Oxidized glutathione fermentation using Saccharomyces cerevisiae engineered for glutathione metabolism

  Kentaro Kiriyama, Kiyotaka Y. Hara, Akihiko Kondo

  Aug. 2013, Applied Microbiology and Biotechnology, 97(16) (16), 7399 - 7404, English

  [Refereed]

  Scientific journal


• Biochemical characterization of a thermostable beta-1,3-xylanase from the hyperthermophilic eubacterium, Thermotoga neapolitana strain DSM 4359

  Fumiyoshi Okazaki, Nanami Nakashima, Chiaki Ogino, Yutaka Tamaru, Akihiko Kondo

  Aug. 2013, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(15) (15), 6749 - 6757, English

  [Refereed]

  Scientific journal


• Creation of endoglucanase-secreting Streptomyces lividans for enzyme production using cellulose as the carbon source

  Shuhei Noda, Yoshifumi Kawai, Takaya Miyazaki, Tsutomu Tanaka, Akihiko Kondo

  Jul. 2013, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(13) (13), 5711 - 5720, English

  [Refereed]

  Scientific journal


• Regulation of central carbon metabolism in Saccharomyces cerevisiae by metabolic inhibitors

  Fumio Matsuda, Tomokazu Shirai, Jun Ishii, Akihiko Kondo

  Jul. 2013, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 116(1) (1), 59 - 64, English

  [Refereed]

  Scientific journal


• Production of lipids containing high levels of docosahexaenoic acid from empty palm fruit bunches by Aurantiochytrium sp. KRS101

  Won-Kyung Hong, Anna Yu, Sun-Yeon Heo, Baek-Rock Oh, Chul Ho Kim, Jung-Hoon Sohn, Ji-Won Yang, Akihiko Kondo, Jeong-Woo Seo

  Jul. 2013, Bioprocess and Biosystems Engineering, 36(7) (7), 959 - 963, English

  [Refereed]

  Scientific journal


• Aqueous size-exclusion chromatographic method for the quantification of cyanobacterial native glycogen

  Yoshihiro Izumi, Shimpei Aikawa, Fumio Matsuda, Tomohisa Hasunuma, Akihiko Kondo

  Jul. 2013, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 930, 90 - 97, English

  [Refereed]

  Scientific journal


• Direct conversion of Spirulina to ethanol without pretreatment or enzymatic hydrolysis processes

  Shimpei Aikawa, Ancy Joseph, Ryosuke Yamada, Yoshihiro Izumi, Takahiro Yamagishi, Fumio Matsuda, Hiroshi Kawai, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo

  Jun. 2013, ENERGY & ENVIRONMENTAL SCIENCE, 6(6) (6), 1844 - 1849, English

  [Refereed]

  Scientific journal


• バイオマスプラスチックとバイオリファイナリー

  OKAI NAOKO, KONDO AKIHIKO

  日本工業出版, Jun. 2013, 配管技術, 55(7) (7), 30 - 35, Japanese

  Scientific journal


• Targeting cancer cell-specific RNA interference by siRNA delivery using a complex carrier of affibody-displaying bio-nanocapsules and liposomes

  Yuya Nishimura, Hiroaki Mieda, Jun Ishii, Chiaki Ogino, Toshinobu Fujiwara, Akihiko Kondo

  Jun. 2013, JOURNAL OF NANOBIOTECHNOLOGY, 11, 19, English

  [Refereed]

  Scientific journal


• Synergetic effect of yeast cell-surface expression of cellulase and expansin-like protein on direct ethanol production from cellulose

  Akihiko Kondo

  Jun. 2013, Microbial Cell Factories, 12(1) (1), 66, English

  [Refereed]

  Scientific journal


• Rapid, Facile Detection of Heterodimer Partners for Target Human G-Protein-Coupled Receptors Using a Modified Split-Ubiquitin Membrane Yeast Two-Hybrid System

  Yasuyuki Nakamura, Jun Ishii, Akihiko Kondo

  Jun. 2013, PLOS ONE, 8(6) (6), e66793, English

  [Refereed]

  Scientific journal


• Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion

  Akihiko Kondo

  Jun. 2013, Journal of Experimental Botany, 64(10) (10), 2943 - 2954, English

  [Refereed]

  Scientific journal


• Characterization of a Squalene Synthase from the Thraustochytrid Microalga Aurantiochytrium sp KRS101

  Won-Kyung Hong, Sun-Yeon Heo, Hye-Mi Park, Chul Ho Kim, Jung-Hoon Sohn, Akihiko Kondo, Jeong-Woo Seo

  Jun. 2013, JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 23(6) (6), 759 - 765, English

  [Refereed]

  Scientific journal


• P-Hydroxycinnamic acid production directly from cellulose using endoglucanase- and tyrosine ammonia lyase-expressing Streptomyces lividans

  Yoshifumi Kawai, Shuhei Noda, Chiaki Ogino, Yasunobu Takeshima, Naoko Okai, Tsutomu Tanaka, Akihiko Kondo

  May 2013, Microbial Cell Factories, 12(1) (1), 45, English

  [Refereed]

  Scientific journal


• High-level production of mature active-form Streptomyces mobaraensis transglutaminase via pro-transglutaminase processing using Streptomyces lividans as a host

  Shuhei Noda, Takaya Miyazaki, Tsutomu Tanaka, Ogino Chiaki, Akihiko Kondo

  May 2013, Biochemical Engineering Journal, 74, 76 - 80, English

  [Refereed]

  Scientific journal


• Utilization of Lactic Acid Bacterial Genes in Synechocystis sp PCC 6803 in the Production of Lactic Acid

  Ancy Joseph, Shimpei Aikawa, Kengo Sasaki, Yota Tsuge, Fumio Matsuda, Tsutomu Tanaka, Akihiko Kondo

  May 2013, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 77(5) (5), 966 - 970, English

  [Refereed]

  Scientific journal


• Sidewall modification of multiwalled carbon nanotubes by Allivum sativum (garlic) and its effect on the deposition of gold nanoparticles

  Sarvesh Kumar Srivastava, Ryosuke Yamada, Chiaki Ogino, Akihiko Kondo

  May 2013, CARBON, 56, 309 - 316, English

  [Refereed]

  Scientific journal


• Production of biodiesel from plant oil hydrolysates using an Aspergillus oryzae whole-cell biocatalyst highly expressing Candida antarctica lipase B

  Daisuke Adachi, Shinji Hama, Kazunori Nakashima, Takayuki Bogaki, Chiaki Ogino, Akihiko Kondo

  May 2013, BIORESOURCE TECHNOLOGY, 135, 410 - 416, English

  [Refereed]

  Scientific journal


• Enzymatic production of biodiesel from waste cooking oil in a packed-bed reactor: An engineering approach to separation of hydrophilic impurities

  Shinji Hama, Ayumi Yoshida, Naoki Tamadani, Hideo Noda, Akihiko Kondo

  May 2013, BIORESOURCE TECHNOLOGY, 135, 417 - 421, English

  [Refereed]

  Scientific journal


• Enzymatic biodiesel production: An overview of potential feedstocks and process development

  Shinji Hama, Akihiko Kondo

  May 2013, BIORESOURCE TECHNOLOGY, 135, 386 - 395, English

  [Refereed]

  Scientific journal


• Display of active beta-glucosidase on the surface of Schizosaccharomyces pombe cells using novel anchor proteins

  Tsutomu Tanaka, Sayoko Matsumoto, Mari Yamada, Ryosuke Yamada, Fumio Matsuda, Akihiko Kondo

  May 2013, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(10) (10), 4343 - 4352, English

  [Refereed]

  Scientific journal


• Cell recycle batch fermentation of high-solid lignocellulose using a recombinant cellulase-displaying yeast strain for high yield ethanol production in consolidated bioprocessing

  Yuki Matano, Tomohisa Hasunuma, Akihiko Kondo

  May 2013, BIORESOURCE TECHNOLOGY, 135, 403 - 409, English

  [Refereed]

  Scientific journal


• Bioethanol production, using carbohydrate-rich microalgae biomass as feedstock

  Shih-Hsin Ho, Shu-Wen Huang, Chun-Yen Chen, Tomohisa Hasunuma, Akihiko Kondo, Jo-Shu Chang

  May 2013, BIORESOURCE TECHNOLOGY, 135, 191 - 198, English

  [Refereed]

  Scientific journal


• A robust whole-cell biocatalyst that introduces a thermo- and solvent-tolerant lipase into Aspergillus oryzae cells: Characterization and application to enzymatic biodiesel production

  Daisuke Adachi, FookHee Koh, Shinji Hama, Chiaki Ogino, Akihiko Kondo

  May 2013, ENZYME AND MICROBIAL TECHNOLOGY, 52(6-7) (6-7), 331 - 335, English

  [Refereed]

  Scientific journal


• A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of their application to consolidated bioprocessing technology

  Tomohisa Hasunuma, Fumiyoshi Okazaki, Naoko Okai, Kiyotaka Y. Hara, Jun Ishii, Akihiko Kondo

  May 2013, BIORESOURCE TECHNOLOGY, 135, 513 - 522, English

  [Refereed]

  Scientific journal


• Genome mining and motif modifications of glycoside hydrolase family 1 members encoded by Geobacillus kaustophilus HTA426 provide thermostable 6-phospho-β-glycosidase and β-fucosidase

  Hirokazu Suzuki, Fumiyoshi Okazaki, Akihiko Kondo, Ken-Ichi Yoshida

  Apr. 2013, Applied Microbiology and Biotechnology, 97(7) (7), 2929 - 2938, English

  [Refereed]

  Scientific journal


• Evaluation of control mechanisms for Saccharomyces cerevisiae central metabolic reactions using metabolome data of eight single-gene deletion mutants

  Tomokazu Shirai, Fumio Matsuda, Mami Okamoto, Akihiko Kondo

  Apr. 2013, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(8) (8), 3569 - 3577, English

  [Refereed]

  Scientific journal


• 電気化学システムを用いた模擬生ごみからの水素発酵の制御

  SASAKI KENGO, SASAKI DAISUKE, 森田 仁彦, 五十嵐 泰夫, KONDO AKIHIKO

  水素エネルギー協会, Mar. 2013, 水素エネルギーシステム, 38(1) (1), 33 - 37, Japanese

  Scientific journal


• Simultaneous improvement of saccharification and ethanol production from crystalline cellulose by alleviation of irreversible adsorption of cellulase with a cell surface-engineered yeast strain

  Yuki Matano, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2013, Applied Microbiology and Biotechnology, 97(5) (5), 2231 - 2237, English

  [Refereed]

  Scientific journal


• Reduction of furan derivatives by overexpressing NADH-dependent Adh1 improves ethanol fermentation using xylose as sole carbon source with Saccharomyces cerevisiae harboring XR-XDH pathway

  Jun Ishii, Kazuya Yoshimura, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2013, Applied Microbiology and Biotechnology, 97(6) (6), 2597 - 2607, English

  [Refereed]

  Scientific journal


• Granting specificity for breast cancer cells using a hepatitis B core particle with a HER2-targeted affibody molecule

  Yuya Nishimura, Wakiko Mimura, Izzat Fahimuddin Mohamed Suffian, Tomokazu Amino, Jun Ishii, Chiaki Ogino, Akihiko Kondo

  Mar. 2013, JOURNAL OF BIOCHEMISTRY, 153(3) (3), 251 - 256, English

  [Refereed]

  Scientific journal


• An affinity chromatography method used to purify His-tag-displaying bio-nanocapsules

  Akihiko Kondo

  Mar. 2013, Journal of Virological Methods, 189(2) (2), 393 - 396, English

  [Refereed]

  Scientific journal


• イオン液体前処理バイオマスを用いたエタノール同時糖化発酵

  OGINO CHIAKI, YAMADA RYOSUKE, KONDO AKIHIKO

  Feb. 2013, 化学工業, 64(2) (2), 52 - 55, Japanese

  Scientific journal


• The production of 1,3-propanediol from mixtures of glycerol and glucose by a Klebsiella pneumoniae mutant deficient in carbon catabolite repression

  Baek-Rock Oh, Won-Kyung Hong, Sun-Yeon Heo, Lian Hua Luo, Akihiko Kondo, Jeong-Woo Seo, Chul Ho Kim

  Feb. 2013, BIORESOURCE TECHNOLOGY, 130, 719 - 724, English

  [Refereed]

  Scientific journal


• Implementation of a transhydrogenase-like shunt to counter redox imbalance during xylose fermentation in Saccharomyces cerevisiae

  Hiroyuki Suga, Fumio Matsuda, Tomohisa Hasunuma, Jun Ishii, Akihiko Kondo

  Feb. 2013, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(4) (4), 1669 - 1678, English

  [Refereed]

  Scientific journal


• Biogenic synthesis and characterization of gold nanoparticles by Escherichia coli K12 and its heterogeneous catalysis in degradation of 4-nitrophenol

  Sarvesh Kumar Srivastava, Ryosuke Yamada, Chiaki Ogino, Akihiko Kondo

  Feb. 2013, NANOSCALE RESEARCH LETTERS, 8(1) (1), 70, English

  [Refereed]

  Scientific journal


• An integrative process model of enzymatic biodiesel production through ethanol fermentation of brown rice followed by lipase-catalyzed ethanolysis in a water-containing system

  Daisuke Adachi, Risa Koda, Shinji Hama, Ryosuke Yamada, Kazunori Nakashima, Chiaki Ogino, Akihiko Kondo

  Feb. 2013, ENZYME AND MICROBIAL TECHNOLOGY, 52(2) (2), 118 - 122, English

  [Refereed]

  Scientific journal


• An automated system for high-throughput single cell-based breeding

  Nobuo Yoshimoto, Akiko Kida, Xu Jie, Masaya Kurokawa, Masumi Iijima, Tomoaki Niimi, Andres D. Maturana, Itoshi Nikaido, Hiroki R. Ueda, Kenji Tatematsu, Katsuyuki Tanizawa, Akihiko Kondo, Ikuo Fujii, Shun'ichi Kuroda

  Feb. 2013, SCIENTIFIC REPORTS, 3, 1191, English

  [Refereed]

  Scientific journal


• Ability of a perfluoropolymer membrane to tolerate by-products of ethanol fermentation broth from dilute acid-pretreated rice straw

  Kengo Sasaki, Fumio Matsuda, Tomohisa Hasunuma, Chiaki Ogino, Masakatsu Urairi, Kazuhito Yoshida, Akihiko Kondo

  Jan. 2013, Biochemical Engineering Journal, 70, 135 - 139, English

  [Refereed]

  Scientific journal


• Gene expression cross-profiling in genetically modified industrial Saccharomyces cerevisiae strains during high-temperature ethanol production from xylose

  Ku Syahidah Ku Ismail, Takatoshi Sakamoto, Haruyo Hatanaka, Tomohisa Hasunuma, Akihiko Kondo

  Jan. 2013, Journal of Biotechnology, 163(1) (1), 50 - 60, English

  [Refereed]

  Scientific journal


• Regenerative Medicine for Epilepsy: From Basic Research to Clinical Application

  Akihiko Kondo

  2013, International Journal of Molecular Sciences

  Scientific journal


• Neuroprotective Effects of Liraglutide for Stroke Model of Rats

  Akihiko Kondo

  2013, International Journal of Molecular Sciences

  Scientific journal


• Biorefineries

  Jong-Moon Park, Akihiko Kondo, Jo-Shu Chang, C. Perry Chou, Pierre Monsan

  Elsevier Ltd, 2013, Bioresource Technology, 135, 1, English

  [Refereed]

  Scientific journal


• Direct cadaverine production from cellobiose using beta-glucosidase displaying Escherichia coli

  Naoki Ikeda, Mari Miyamoto, Noriko Adachi, Mariko Nakano, Tsutomu Tanaka, Akihiko Kondo

  2013, AMB EXPRESS, 3, English

  [Refereed]

  Scientific journal


• Particle size for photocatalytic activity of anatase TiO2 nanosheets with highly exposed {001} facets

  Akihiko Kondo

  2013, RSC Advances, 3(1) (1), 19268 - 19271, English

  [Refereed]

  Scientific journal


• Green synthesis of Au, Pd and Au@ Pd core-shell nanoparticles via a tryptophan induced supramolecular interface

  Sarvesh Kumar Srivastava, Takashi Hasegawa, Ryosuke Yamada, Chiaki Ogino, Minoru Mizuhata, Akihiko Kondo

  2013, RSC ADVANCES, 3(40) (40), 18367 - 18372, English

  [Refereed]

  Scientific journal


• An approach for dynamical network reconstruction of simple netswork motifs

  NAKATSUI Masahiko, ARAKI Michihiro, KONDO Akihiko

  2013, BMC System Biology, 7(suppl 6) (suppl 6), 54, English

  [Refereed]

  Scientific journal


• Single-step production of polyhydroxybutyrate from starch by using alpha-amylase cell-surface displaying system of Corynebacterium glutamicum

  Yuyang Song, Ken'ichiro Matsumoto, Tsutomu Tanaka, Akihiko Kondo, Seiichi Taguchi

  Jan. 2013, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 115(1) (1), 12 - 14, English

  [Refereed]

  Scientific journal


• Development of microbial cell factories for bio-refinery through synthetic bioengineering

  Akihiko Kondo, Jun Ishii, Kiyotaka Y. Hara, Tomohisa Hasunuma, Fumio Matsuda

  Jan. 2013, JOURNAL OF BIOTECHNOLOGY, 163(2) (2), 204 - 216, English

  [Refereed]

  Scientific journal


• Characterization and optimization of carbohydrate production from an indigenous microalga Chlorella vulgaris FSP-E

  Shih-Hsin Ho, Shu-Wen Huang, Chun-Yen Chen, Tomohisa Hasunuma, Akihiko Kondo, Jo-Shu Chang

  Elsevier Ltd, 2013, Bioresource Technology, 135, 157 - 165, English

  [Refereed]

  Scientific journal


• Efficient direct ethanol production from cellulose by cellulase- and cellodextrin transporter-co-expressing Saccharomyces cerevisiae

  Ryosuke Yamada, Yuki Nakatani, Chiaki Ogino, Akihiko Kondo

  Springer Verlag, 2013, AMB Express, 3(1) (1), 1 - 7, English

  [Refereed]

  Scientific journal


• Complex carriers of affibody-displaying bio-nanocapsules and composition-varied liposomes for HER2-expressing breast cancer cell-specific protein delivery

  Yuya Nishimura, Jun Ishii, Fumiyoshi Okazaki, Chiaki Ogino, Akihiko Kondo

  Dec. 2012, JOURNAL OF DRUG TARGETING, 20(10) (10), 897 - 905, English

  [Refereed]

  Scientific journal


• An improved bioluminescence-based signaling assay for odor sensing with a yeast expressing a chimeric olfactory receptor

  Yosuke Fukutani, Jun Ishii, Keiichi Noguchi, Akihiko Kondo, Masafumi Yohda

  Dec. 2012, BIOTECHNOLOGY AND BIOENGINEERING, 109(12) (12), 3143 - 3151, English

  [Refereed]

  Scientific journal


• Extracellular glutathione fermentation using engineered Saccharomyces cerevisiae expressing a novel glutathione exporter

  Kentaro Kiriyama, Kiyotaka Y. Hara, Akihiko Kondo

  Nov. 2012, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 96(4) (4), 1021 - 1027, English

  [Refereed]

  Scientific journal


• Development of yeast cell factories for consolidated bioprocessing of lignocellulose to bioethanol through cell surface engineering

  Tomohisa Hasunuma, Akihiko Kondo

  Nov. 2012, BIOTECHNOLOGY ADVANCES, 30(6) (6), 1207 - 1218, English

  [Refereed]

  Scientific journal


• Efficient heterologous expression and secretion in Aspergillus oryzae of a llama variable heavy-chain antibody fragment V-HH against EGFR

  Fumiyoshi Okazaki, Jun-ichi Aoki, Soichiro Tabuchi, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Oct. 2012, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 96(1) (1), 81 - 88, English

  [Refereed]

  Scientific journal


• Continuous production of biodiesel using whole-cell biocatalysts: Sequential conversion of an aqueous oil emulsion into anhydrous product

  Ayumi Yoshida, Shinji Hama, Naoki Tamadani, Hideo Noda, Hideki Fukuda, Akihiko Kondo

  Oct. 2012, BIOCHEMICAL ENGINEERING JOURNAL, 68, 7 - 11, English

  [Refereed]

  Scientific journal


• Enzyme-mediated methodologies for protein modification and bioconjugate synthesis.

  Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo

  Bioconjugates are valuable tools in many fields, including protein engineering and environmental and therapeutic research. Chemical methods are commonly used to synthesize protein-protein and protein-functional molecule bioconjugates because they permit easy tethering through covalent bonds. However, chemical methods often produce heterogeneous products and lead to degradation of protein activity due to random modifications. Recently, a number of techniques for modifying proteins or synthesizing bioconjugates have been reported, including more sophisticated chemical modification methods, utilization of noncovalent affinity, and protein splicing. Enzymatic methods in particular have attracted much attention due to the substrate specificity of enzymes, which enables site-specific tethering of proteins to other proteins or functional molecules. Here, we discuss newly developed methods for protein modification and bioconjugate synthesis that exploit the properties of acyltransferases, ligases, and other enzymes.

  Sep. 2012, Biotechnology journal, 7(9) (9), 1137 - 46, English, International magazine

  [Refereed]

  Scientific journal


• Consolidated bioprocessing and simultaneous saccharification and fermentation of lignocellulose to ethanol with thermotolerant yeast strains

  Tomohisa Hasunuma, Akihiko Kondo

  Sep. 2012, PROCESS BIOCHEMISTRY, 47(9) (9), 1287 - 1294, English

  [Refereed]

  Scientific journal


• Design of superior cell factories for a sustainable biorefinery by synthetic bioengineering

  Tomohisa Hasunuma, Fumio Matsuda, Akihiko Kondo

  Springer Netherlands, Aug. 2012, Systems Metabolic Engineering, 9789400745346, 329 - 348, English

  [Refereed]

  In book


• Suger consumption and ethanol fermentation by transporter-overexpressed xylose metabolizing Saccharomyces cerevisiae harboring a xyloseisomerase pathway

  TANINO T, ITO T, OGINO Chiaki, OHMURA Naoto, OHSHIMA T, KONDO Akihiko

  Four kinds of transporters, HXT1 and HXY7 from Saccharomyces cerevisiae, and GXFI and GXSI from Candida inter- media, were overexpressed in xylose-metabolizing S. cerevisiae harboring a xyloseisomerase-based pathway. Over- expression of transporter enhanced sugar consumption and ethanol production, and GXF1 was efficient for ethanol fermentation from both glucose and xylose.

  The Society for Biotechnology, Japan, Aug. 2012, Journal of Bioscience and Bioengineering, 114(2) (2), 209 - 211, English

  [Refereed]

  Scientific journal


• Recent developments in yeast cell surface display toward extended applications in biotechnology

  Akihiko Kondo

  Aug. 2012, Applied Microbiology and Biotechnology, 95, 577 - 591, English

  [Refereed]

  Scientific journal


• Robust production of gamma-amino butyric acid using recombinant Corynebacterium glutamicum expressing glutamate decarboxylase from Escherichia coli

  Chihiro Takahashi, Junki Shirakawa, Takeyuki Tsuchidate, Naoko Okai, Kazuki Hatada, Hideki Nakayama, Toshihiro Tateno, Chiaki Ogino, Akihiko Kondo

  Aug. 2012, ENZYME AND MICROBIAL TECHNOLOGY, 51(3) (3), 171 - 176, English

  [Refereed]

  Scientific journal


• Adaptation of light-harvesting systems of Arthrospira platensis to light conditions, probed by time-resolved fluorescence spectroscopy

  Seiji Akimoto, Makio Yokono, Fumiya Hamada, Ayaka Teshigahara, Shimpei Aikawa, Akihiko Kondo

  Aug. 2012, BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1817(8) (8), 1483 - 1489, English

  [Refereed]

  Scientific journal


• 合成生物工学によるバイオ燃料生産のための微生物細胞工場の創製

  HASUNUMA TOMOHISA, KONDO AKIHIKO

  日本生物工学会, Jul. 2012, 生物工学会誌, 90(7) (7), 386 - 391, Japanese

  Scientific journal


• Improved identification of agonist-mediated G alpha(i)-specific human G-protein-coupled receptor signaling in yeast cells by flow cytometry

  Jun Ishii, Miyuki Moriguchi, Kiyotaka Y. Hara, Seiji Shibasaki, Hideki Fukuda, Akihiko Kondo

  Jul. 2012, ANALYTICAL BIOCHEMISTRY, 426(2) (2), 129 - 133, English

  [Refereed]

  Scientific journal


• Direct isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display system

  Yuki Soma, Kentaro Inokuma, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo, Masahiro Okamoto, Taizo Hanai

  Jul. 2012, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 114(1) (1), 80 - 85, English

  [Refereed]

  Scientific journal


• Improvements in ethanol production from xylose by mating recombinant xylose-fermenting Saccharomyces cerevisiae strains

  Hiroko Kato, Hiroaki Suyama, Ryosuke Yamada, Tomohisa Hasunuma, Akihiko Kondo

  Jun. 2012, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 94(6) (6), 1585 - 1592, English

  [Refereed]

  Scientific journal


• Improvement of glutathione production by metabolic engineering the sulfate assimilation pathway of Saccharomyces cerevisiae

  Kiyotaka Y. Hara, Kentaro Kiriyama, Akiko Inagaki, Hideki Nakayama, Akihiko Kondo

  Jun. 2012, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 94(5) (5), 1313 - 1319, English

  [Refereed]

  Scientific journal


• Widely targeted metabolic profiling analysis of yeast central metabolites

  Hiroko Kato, Yoshihiro Izumi, Tomohisa Hasunuma, Fumio Matsuda, Akihiko Kondo

  May 2012, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 113(5) (5), 665 - 673, English

  [Refereed]

  Scientific journal


• Transplantation of the GAL regulon into G-protein signaling circuitry in yeast

  Shintaro Ryo, Jun Ishii, Yusuke Iguchi, Nobuo Fukuda, Akihiko Kondo

  May 2012, ANALYTICAL BIOCHEMISTRY, 424(1) (1), 27 - 31, English

  [Refereed]

  Scientific journal


• Site-specific protein labeling with amine-containing molecules using Lactobacillus plantarum sortase

  Takuya Matsumoto, Ryosuke Takase, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  May 2012, BIOTECHNOLOGY JOURNAL, 7(5) (5), 642 - 648, English

  [Refereed]

  Scientific journal


• Sentinel Node Mapping with Thermoresponsive Magnetic Nanoparticles in Rats

  Kunihiko Hiraiwa, Masakazu Ueda, Hiroya Takeuchi, Takashi Oyama, Tomoyuki Irino, Takahisa Yoshikawa, Akihiko Kondo, Yuko Kitagawa

  May 2012, JOURNAL OF SURGICAL RESEARCH, 174(1) (1), 48 - 55, English

  [Refereed]

  Scientific journal


• Repeated fermentation from raw starch using Saccharomyces cerevisiae displaying both glucoamylase and alpha-amylase

  Syun-ichi Yamakawa, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  May 2012, ENZYME AND MICROBIAL TECHNOLOGY, 50(6-7) (6-7), 343 - 347, English

  [Refereed]

  Scientific journal


• Genetic engineering to enhance the Ehrlich pathway and alter carbon flux for increased isobutanol production from glucose by Saccharomyces cerevisiae

  Takashi Kondo, Hironori Tezuka, Jun Ishii, Fumio Matsuda, Chiaki Ogino, Akihiko Kondo

  May 2012, JOURNAL OF BIOTECHNOLOGY, 159(1-2) (1-2), 32 - 37, English

  [Refereed]

  Scientific journal


• Enhanced production of 2,3-butanediol by engineered Bacillus subtilis

  Ranjita Biswas, Masaru Yamaoka, Hideki Nakayama, Takashi Kondo, Ken-ichi Yoshida, Virendra S. Bisaria, Akihiko Kondo

  May 2012, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 94(3) (3), 651 - 658, English

  [Refereed]

  Scientific journal


• Deletion of the PHO13 gene in Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysate in the presence of acetic and formic acids, and furfural

  Keisuke Fujitomi, Tomoya Sanda, Tomohisa Hasunuma, Akihiko Kondo

  May 2012, BIORESOURCE TECHNOLOGY, 111, 161 - 166, English

  [Refereed]

  Scientific journal


• Cell Wall Trapping of Autocrine Peptides for Human G-Protein-Coupled Receptors on the Yeast Cell Surface

  Jun Ishii, Nobuo Yoshimoto, Kenji Tatematsu, Shun'ichi Kuroda, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  May 2012, PLOS ONE, 7(5) (5), e37136, English

  [Refereed]

  Scientific journal


• An energy-saving glutathione production method from low-temperature cooked rice using amylase-expressing Saccharomyces cerevisiae

  Kiyotaka Y. Hara, Songhee Kim, Kentaro Kiriyama, Hideyo Yoshida, Shogo Arai, Jun Ishii, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  May 2012, BIOTECHNOLOGY JOURNAL, 7(5) (5), 686 - 689, English

  [Refereed]

  Scientific journal


• Improved performance of a packed-bed reactor for biodiesel production through whole-cell biocatalysis employing a high-lipase-expression system

  Ayumi Yoshida, Shinji Hama, Naoki Tamadani, Hideki Fukuda, Akihiko Kondo

  Apr. 2012, BIOCHEMICAL ENGINEERING JOURNAL, 63, 76 - 80, English

  [Refereed]

  Scientific journal


• Direct ethanol production from hemicellulosic materials of rice straw by use of an engineered yeast strain codisplaying three types of hemicellulolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells

  Takatoshi Sakamoto, Tomohisa Hasunuma, Yoshimi Hori, Ryosuke Yamada, Akihiko Kondo

  Apr. 2012, JOURNAL OF BIOTECHNOLOGY, 158(4) (4), 203 - 210, English

  [Refereed]

  Scientific journal


• Benzoic acid fermentation from starch and cellulose via a plant-like beta-oxidation pathway in Streptomyces maritimus

  Shuhei Noda, Eiichi Kitazono, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Apr. 2012, MICROBIAL CELL FACTORIES, 11(1) (1), 49, English

  [Refereed]

  Scientific journal


• Synergistic enhancement of glycogen production in Arthrospira platensis by optimization of light intensity and nitrate supply

  Shimpei Aikawa, Yoshihiro Izumi, Fumio Matsuda, Tomohisa Hasunuma, Jo-Shu Chang, Akihiko Kondo

  Mar. 2012, BIORESOURCE TECHNOLOGY, 108, 211 - 215, English

  [Refereed]

  Scientific journal


• Display of cellulases on the cell surface of Saccharomyces cerevisiae for high yield ethanol production from high-solid lignocellulosic biomass

  Yuki Matano, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2012, BIORESOURCE TECHNOLOGY, 108, 128 - 133, English

  [Refereed]

  Scientific journal


• Bioconversion of lignocellulose-derived sugars to ethanol by engineered Saccharomyces cerevisiae

  Anjali Madhavan, Aradhana Srivastava, Akihiko Kondo, Virendra S. Bisaria

  Mar. 2012, CRITICAL REVIEWS IN BIOTECHNOLOGY, 32(1) (1), 22 - 48, English

  [Refereed]

  Scientific journal


• Sortase A-Catalyzed Site-Specific Coimmobilization on Microparticles via Streptavidin

  Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo

  Feb. 2012, LANGMUIR, 28(7) (7), 3553 - 3557, English

  [Refereed]

  Scientific journal


• Development of a glutathione production process from proteinaceous biomass resources using protease-displaying Saccharomyces cerevisiae

  Kiyotaka Y. Hara, Songhee Kim, Hideyo Yoshida, Kentaro Kiriyama, Takashi Kondo, Naoko Okai, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Feb. 2012, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 93(4) (4), 1495 - 1502, English

  [Refereed]

  Scientific journal


• Effect of the α-glucosidase inhibitor miglitol on the glucose profile in Japanese type 2 diabetic patients receiving multiple daily insulin injections

  Akihiko Kondo

  2012, Endocrine Journal

  Scientific journal


• Development of bioethanol production systems for creating a cyclical and cascading utilization method of agricultural waste.

  KITAGUCHI Toshihiro, MITSUHASHI Hiroyuki, YAMAKOSHI Yukiyasu, YAMADA Ryosuke, KONDO Akihiko, MIYASHITA Kazuo

  北海道十勝地方などの大規模畑作地域からビートトップ、麦わら、ジャガイモ地上部、豆殻などのセルロース系農産廃棄物が大量に排出されており、適切な処理が望まれている。それら廃棄物にはカロテノイドなどの高付加価値物質を含むものがある。これを機能性食品成分として回収し、残渣を原料とする高効率なバイオエタノール製造技術体系を確立することを目的とし、高付加価値物質の機能性、保存性、高効率バイオエタノール製造のための前処理特性、高温耐性酵母に関する検討を行った。その結果、ビートトップから抽出されたカロテノイド、特にネオキサンチンは抗肥満性があること、カロテノイドはエタノール抽出液中で遮光および低温下において長期間の保存に耐えること、バイオエタノール製造のための前処理では、麦桿の糖化効率は非常に高かったが、大豆殻は改善の余地があることがわかった。また、42℃でもエタノール発酵が可能な高温耐性酵母が得られた。

  Japan Society of Material Cycles and Waste Management, 2012, Proceedings of the Annual Conference of Japan Society of Material Cycles and Waste Management, 23, 389 - 389


• Preparation of aqueous dispersible styrene-maleic amide encapsulated CoAl 2O 4 nanocrystals using supercritical water flow type apparatus

  D. Rangappa, S. Ohara, S. Takami, T. Naka, A. Kondo, M. Ishii, T. Kobayashi, T. Adschiri

  2012, Materials Research Innovations, 16(1) (1), 30 - 37

  Scientific journal


• Preface to the special edition

  M. Ueda, A. Kondo

  2012, Seibutsu-kogaku Kaishi, 90(7) (7), 381-, English

  [Refereed]


• バイオリファイナリーとバイオプラスチック

  OKAI NAOKO, HASUNUMA TOMOHISA, KONDO AKIHIKO

  2012, ペトロテック, 35(10) (10), 700 - 706, Japanese

  Scientific journal


• The N-terminal replacement of an olfactory receptor for the development of a Yeast-based biomimetic odor sensor

  Yosuke Fukutani, Tomoko Nakamura, Maiko Yorozu, Jun Ishii, Akihiko Kondo, Masafumi Yohda

  Jan. 2012, BIOTECHNOLOGY AND BIOENGINEERING, 109(1) (1), 205 - 212, English

  [Refereed]

  Scientific journal


• Protein-encapsulated bio-nanocapsules production with ER membrane localization sequences

  Yuya Nishimura, Takuya Shishido, Jun Ishii, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Jan. 2012, JOURNAL OF BIOTECHNOLOGY, 157(1) (1), 124 - 129, English

  [Refereed]

  Scientific journal


• Production of Streptoverticillium cinnamoneum transglutaminase and cinnamic acid by recombinant Streptomyces lividans cultured on biomass-derived carbon sources

  Shuhei Noda, Takaya Miyazaki, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Jan. 2012, BIORESOURCE TECHNOLOGY, 104, 648 - 651, English

  [Refereed]

  Scientific journal


• Growth of the oleaginous microalga Aurantiochytrium sp KRS101 on cellulosic biomass and the production of lipids containing high levels of docosahexaenoic acid

  Won-Kyung Hong, Chul Ho Kim, Dina Rairakhwada, Seonghun Kim, Byung-Ki Hur, Akihiko Kondo, Jeong-Woo Seo

  Jan. 2012, BIOPROCESS AND BIOSYSTEMS ENGINEERING, 35(1-2) (1-2), 129 - 133, English

  [Refereed]

  Scientific journal


• Construction of an artificial pathway for isobutanol biosynthesis in the cytosol of saccharomyces cerevisiae

  Fumio Matsuda, Takashi Kondo, Kengo Ida, Hironori Tezuka, Jun Ishii, Akihiko Kondo

  2012, Bioscience, Biotechnology and Biochemistry, 76(11) (11), 2139 - 2141, English

  [Refereed]

  Scientific journal


• Coupling Stimuli-Responsive Magnetic Nanoparticles with Antibody-Antigen Detection in Immunoassays

  Hirokazu Nagaoka, Yasunobu Sato, Xiaomao Xie, Hideyuki Hata, Masaru Eguchi, Nobulci Sakurai, Takeshi Watanabe, Hiroshi Saitoh, Akihiko Kondo, Satoru Sugita, Noriyuki Ohnishi

  Dec. 2011, ANALYTICAL CHEMISTRY, 83(24) (24), 9197 - 9200, English

  [Refereed]

  Scientific journal


• Process engineering and optimization of glycerol separation in a packed-bed reactor for enzymatic biodiesel production

  Shinji Hama, Sriappareddy Tamalampudi, Ayumi Yoshida, Naoki Tamadani, Nobuyuki Kuratani, Hideo Noda, Hideki Fukuda, Akihiko Kondo

  Nov. 2011, BIORESOURCE TECHNOLOGY, 102(22) (22), 10419 - 10424, English

  [Refereed]

  Scientific journal


• Isolation of a novel promoter for efficient protein expression by Aspergillus oryzae in solid-state culture

  Hiroki Bando, Hiromoto Hisada, Hiroki Ishida, Yoji Hata, Yoshio Katakura, Akihiko Kondo

  Nov. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 92(3) (3), 561 - 569, English

  [Refereed]

  Scientific journal


• Development of a novel aptamer-based sensing system using atomic force microscopy

  Yusuke Miyachi, Chiaki Ogino, Tomokazu Amino, Akihiko Kondo

  Nov. 2011, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 112(5) (5), 511 - 514, English

  [Refereed]

  Scientific journal


• Comparative Profiling Analysis of Central Metabolites in Euglena gracilis under Various Cultivation Conditions

  Fumio Matsuda, Masahiro Hayashi, Akihiko Kondo

  Nov. 2011, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 75(11) (11), 2253 - 2256, English

  [Refereed]

  Scientific journal


• Homo-D-lactic acid production from mixed sugars using xylose-assimilating operon-integrated Lactobacillus plantarum

  Shogo Yoshida, Kenji Okano, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Oct. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 92(1) (1), 67 - 76, English

  [Refereed]

  Scientific journal


• Amplification of agonist stimulation of human G-protein-coupled receptor signaling in yeast

  Nobuo Fukuda, Jun Ishii, Misato Kaishima, Akihiko Kondo

  Oct. 2011, ANALYTICAL BIOCHEMISTRY, 417(2) (2), 182 - 187, English

  [Refereed]

  Scientific journal


• Repeated-batch fermentation of lignocellulosic hydrolysate to ethanol using a hybrid Saccharomyces cerevisiae strain metabolically engineered for tolerance to acetic and formic acids

  Tomoya Sanda, Tomohisa Hasunuma, Fumio Matsuda, Akihiko Kondo

  Sep. 2011, BIORESOURCE TECHNOLOGY, 102(17) (17), 7917 - 7924, English

  [Refereed]

  Scientific journal


• Improved homo l-lactic acid fermentation from xylose by abolishment of the phosphoketolase pathway and enhancement of the pentose phosphate pathway in genetically modified xylose-assimilating Lactococcus lactis

  Satoru Shinkawa, Kenji Okano, Shogo Yoshida, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Sep. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 91(6) (6), 1537 - 1544, English

  [Refereed]

  Scientific journal


• Creation of a Cellooligosaccharide-Assimilating Escherichia coli Strain by Displaying Active Beta-Glucosidase on the Cell Surface via a Novel Anchor Protein

  Tsutomu Tanaka, Hitomi Kawabata, Chiaki Ogino, Akihiko Kondo

  Sep. 2011, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77(17) (17), 6265 - 6270, English

  [Refereed]

  Scientific journal


• Co-fermentation of cellulose/xylan using engineered industrial yeast strain OC-2 displaying both beta-glucosidase and beta-xylosidase

  Satoshi Saitoh, Tsutomu Tanaka, Akihiko Kondo

  Sep. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 91(6) (6), 1553 - 1559, English

  [Refereed]

  Scientific journal


• G gamma recruitment system incorporating a novel signal amplification circuit to screen transient protein-protein interactions

  Nobuo Fukuda, Jun Ishii, Akihiko Kondo

  Sep. 2011, FEBS JOURNAL, 278(17) (17), 3086 - 3094, English

  [Refereed]

  Scientific journal


• Engineering strategy of yeast metabolism for higher alcohol production

  Fumio Matsuda, Chikara Furusawa, Takashi Kondo, Jun Ishii, Hiroshi Shimizu, Akihiko Kondo

  Sep. 2011, MICROBIAL CELL FACTORIES, 10, English

  [Refereed]

  Scientific journal


• Variation in Biomass Properties among Rice Diverse Cultivars

  Fumio Matsuda, Masanori Yamasaki, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Aug. 2011, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 75(8) (8), 1603 - 1605, English

  [Refereed]

  Scientific journal


• Enzymatic glutathione production using metabolically engineered Saccharomyces cerevisiae as a whole-cell biocatalyst

  Hideyo Yoshida, Kiyotaka Y. Hara, Kentaro Kiriyama, Hideki Nakayama, Fumiyoshi Okazaki, Fumio Matsuda, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Aug. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 91(4) (4), 1001 - 1006, English

  [Refereed]

  Scientific journal


• Expression, crystallization and preliminary X-ray diffraction studies of thermostable beta-1,3-xylanase from Thermotoga neapolitana strain DSM 4359

  Fumiyoshi Okazaki, Chiaki Ogino, Akihiko Kondo, Bunzo Mikami, Yoichi Kurebayashi, Hiroki Tsuruta

  Jul. 2011, ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 67, 779 - 781, English

  [Refereed]

  Scientific journal


• Transesterification of phosphatidylcholine in sn-1 position through direct use of lipase-producing Rhizopus oryzae cells as whole-cell biocatalyst

  Shinji Hama, Kazunori Miura, Ayumi Yoshida, Hideo Noda, Hideki Fukuda, Akihiko Kondo

  Jun. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 90(5) (5), 1731 - 1738, English

  [Refereed]

  Scientific journal


• Near Infrared Spectroscopy As High-Throughput Technology for Screening of Xylose-Fermenting Recombinant Saccharomyces cerevisiae Strains

  Hiroyuki Morita, Tomohisa Hasunuma, Maria Vassileva, Roumiana Tsenkova, Akihiko Kondo

  Jun. 2011, ANALYTICAL CHEMISTRY, 83(11) (11), 4023 - 4029, English

  [Refereed]

  Scientific journal


• Enzymatic packed-bed reactor integrated with glycerol-separating system for solvent-free production of biodiesel fuel

  Shinji Hama, Sriappareddy Tamalampudi, Ayumi Yoshida, Naoki Tamadani, Nobuyuki Kuratani, Hideo Noda, Hideki Fukuda, Akihiko Kondo

  Jun. 2011, BIOCHEMICAL ENGINEERING JOURNAL, 55(1) (1), 66 - 71, English

  [Refereed]

  Scientific journal


• Development of an Aspergillus oryzae whole-cell biocatalyst coexpressing triglyceride and partial glyceride lipases for biodiesel production

  Daisuke Adachi, Shinji Hama, Takao Numata, Kazunori Nakashima, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Jun. 2011, BIORESOURCE TECHNOLOGY, 102(12) (12), 6723 - 6729, English

  [Refereed]

  Scientific journal


• Highly efficient biodiesel production by a whole-cell biocatalyst employing a system with high lipase expression in Aspergillus oryzae

  Tomohiro Takaya, Risa Koda, Daisuke Adachi, Kazunori Nakashima, Junpei Wada, Takayuki Bogaki, Chiaki Ogino, Akihiko Kondo

  May 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 90(3) (3), 1171 - 1177, English

  [Refereed]

  Scientific journal


• Efficient fermentation of xylose to ethanol at high formic acid concentrations by metabolically engineered Saccharomyces cerevisiae

  Tomohisa Hasunuma, Kyung-mo Sung, Tomoya Sanda, Kazuya Yoshimura, Fumio Matsuda, Akihiko Kondo

  May 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 90(3) (3), 997 - 1004, English

  [Refereed]

  Scientific journal


• Glutamate production from beta-glucan using endoglucanase-secreting Corynebacterium glutamicum

  Takeyuki Tsuchidate, Toshihiro Tateno, Naoko Okai, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  May 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 90(3) (3), 895 - 901, English

  [Refereed]

  Scientific journal


• Cinnamic acid production using Streptomyces lividans expressing phenylalanine ammonia lyase

  Shuhei Noda, Takaya Miyazaki, Takanori Miyoshi, Michiru Miyake, Naoko Okai, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  May 2011, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 38(5) (5), 643 - 648, English

  [Refereed]

  Scientific journal


• Intrahepatic lipid content is linked to insulin resistance in obese subjects

  Yukiyoshi Sada, Takuyuki Katabami, Shiko Asai, Tomoko Sato, Kentarou Furukawa, Satoshi Ishii, Hiroyuki Kato, Hidetoshi Kobayashi, Akihiko Kondo, Akio Ohta, Yasuo Nakajima, Yasushi Tanaka

  Apr. 2011, Obesity Research and Clinical Practice, 5(2) (2), e129 - e136, English

  [Refereed]

  Scientific journal


• Water activity dependence of performance of surface-displayed lipase in yeast cells: A unique water requirement for enzymatic synthetic reaction in organic media

  Ayumi Yoshida, Shinji Hama, Kazunori Nakashima, Akihiko Kondo

  Apr. 2011, ENZYME AND MICROBIAL TECHNOLOGY, 48(4-5) (4-5), 334 - 338, English

  [Refereed]

  Scientific journal


• Direct ethanol production from cassava pulp using a surface-engineered yeast strain co-displaying two amylases, two cellulases, and beta-glucosidase

  Waraporn Apiwatanapiwat, Yoshinori Murata, Akihiko Kosugi, Ryosuke Yamada, Akihiko Kondo, Takamitsu Arai, Prapassorn Rugthaworn, Yutaka Mori

  Apr. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 90(1) (1), 377 - 384, English

  [Refereed]

  Scientific journal


• Direct and efficient ethanol production from high-yielding rice using a Saccharomyces cerevisiae strain that express amylases

  Ryosuke Yamada, Syun-ichi Yamakawa, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Apr. 2011, ENZYME AND MICROBIAL TECHNOLOGY, 48(4-5) (4-5), 393 - 396, English

  [Refereed]

  Scientific journal


• Site-specific tetrameric streptavidin-protein conjugation using sortase A

  Takuya Matsumoto, Shiori Sawamoto, Takayuki Sakamoto, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  Mar. 2011, JOURNAL OF BIOTECHNOLOGY, 152(1-2) (1-2), 37 - 42, English

  [Refereed]

  Scientific journal


• Efficient and direct glutathione production from raw starch using engineered Saccharomyces cerevisiae

  Hideyo Yoshida, Shogo Arai, Kiyotaka Y. Hara, Ryosuke Yamada, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Mar. 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 89(5) (5), 1417 - 1422, English

  [Refereed]

  Scientific journal


• Striatal Stimulation Nurtures Endogenous Neurogenesis and Angiogenesis in Chronic-Phase Ischemic Stroke Rats

  Akihiko Kondo

  2011, Cell Transplantation

  Scientific journal


• Urinary 8-OHdG Elevations in a Partial Lesion Rat Model of Parkinson's Disease Correlate With Behavioral Symptoms and Nigrostriatal Dopaminergic Depletion

  Akihiko Kondo

  2011, Journal of Cellular Physiology

  Scientific journal


• Versive seizures in occipital lobe epilepsy: Lateralizing value and pathophysiology

  Akihiko Kondo

  2011, Epilepsy Research

  Scientific journal


• INCREASED AND DECREASED CENTRAL TYPE BENZODIAZEPINE RECEPTOR BINDINGS ASSOCIATED WITH SEIZURE OUTCOMES IN EPILEPTOGENIC CAVERNOUS ANGIOMA AND FRONTAL CORTICAL DYSPLASIA

  Akihiko Kondo

  2011, Epilepsia

  Scientific journal


• Clinical significance of ictal high frequency oscillations in medial temporal lobe epilepsy

  Akihiko Kondo

  2011, Clinical Neurophysiology

  Scientific journal


• BDNF-secreting capsule exerts neuroprotective effects on epilepsy model of rats

  Akihiko Kondo

  2011, Brain Research

  Scientific journal


• Aromatic chemicals production using phenylalnine ammonia lyase expressing Streptomyces lividans

  Chiaki Ogino, Shuhei Noda, Akihiko Kondo, Naoko Okai, Tsutomu Tanaka

  2011, 2011 Defense Science Research Conference and Expo, DSR 2011, English

  [Refereed]

  International conference proceedings


• Direct ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiae with optimized cellulase expression

  Ryosuke Yamada, Naho Taniguchi, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  BioMed Central Ltd., 2011, Biotechnology for Biofuels, 4(1) (1), 8, English

  [Refereed]

  Scientific journal


• Direct ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiae with optimized cellulase expression

  YAMADA Ryosuke, TANIGUCHI N, TANAKA Tsutomu, OGINO Chiaki, FUKUDA Hideki, KONDO Akihiko

  2011, Biotechnology for Biofuels, 4:8, English

  [Refereed]

  Scientific journal


• Direct bioethanol production from cellulose by the combination of cellulase-displaying yeast and ionic liquid pretreatment

  Kazunori Nakashima, Koji Yamaguchi, Naho Taniguchi, Shogo Arai, Ryosuke Yamada, Satoshi Katahira, Nobuhiro Ishida, Haruo Takahashi, Chiaki Ogino, Akihiko Kondo

  2011, GREEN CHEMISTRY, 13(10) (10), 2948 - 2953, English

  [Refereed]

  Scientific journal


• Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae

  Tomohisa Hasunuma, Tomoya Sanda, Ryosuke Yamada, Kazuya Yoshimura, Jun Ishii, Akihiko Kondo

  Jan. 2011, MICROBIAL CELL FACTORIES, 10, English

  [Refereed]

  Scientific journal


• Enzyme-Mediated Site-Specific Antibody-Protein Modification Using a ZZ Domain as a Linker

  Takayuki Sakamoto, Shiori Sawamoto, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  Dec. 2010, BIOCONJUGATE CHEMISTRY, 21(12) (12), 2227 - 2233, English

  [Refereed]

  Scientific journal


• Construction of a xylose-metabolizing yeast by genome integration of xylose isomerase gene and investigation of the effect of xylitol on fermentation

  Takanori Tanino, Atsushi Hotta, Tomonori Ito, Jun Ishii, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Naoto Ohmura, Takayuki Ohshima, Akihiko Kondo

  Nov. 2010, Applied Microbiology and Biotechnology, 88(5) (5), 1215 - 1221, English

  [Refereed]

  Scientific journal


• Construction of a xylose-metabolizing Saccharomyces cerevisiae by integration of xylose isomerase gene into the genome and investigation of the effect of xylITOl on fermentation

  Takanori Tanino, Atsushi Hotta, Tomonori Ito, Jun Ishii, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Naoto Ohmura, Takayuki Ohima, Akihiko Kondo

  Nov. 2010, Applied Microbiology and Biotechnology, 88, 1215-1221, English

  [Refereed]

  Scientific journal


• Stress-chain based micromechanics of sand with grain shape effect

  Kenichi Maeda, Hirotaka Sakai, Akihiko Kondo, Tomoyo Yamaguchi, Masatoshi Fukuma, Emi Nukudani

  Oct. 2010, Granular Matter, 12(5) (5), 499 - 505, English

  [Refereed]

  Scientific journal


• Over-production of various secretory-form proteins in Streptomyces lividans

  Shuhei Noda, Yuko Ito, Nobuaki Shimizu, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Oct. 2010, PROTEIN EXPRESSION AND PURIFICATION, 73(2) (2), 198 - 202, English

  [Refereed]

  Scientific journal


• Affibody-displaying bionanocapsules for specific drug delivery to HER2-expressing cancer cells

  Takuya Shishido, Hiroaki Mieda, Sang Youn Hwang, Yuya Nishimura, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Oct. 2010, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 20(19) (19), 5726 - 5731, English

  [Refereed]

  Scientific journal


• Ethanolysis of rapeseed oil to produce biodiesel fuel catalyzed by Fusarium heterosporum lipase-expressing fungus immobilized whole-cell biocatalysts

  Risa Koda, Takao Numata, Shinji Hama, Sriappareddy Tamalampudi, Kazunori Nakashima, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Sep. 2010, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 66(1-2) (1-2), 101 - 104, English

  [Refereed]

  Scientific journal


• Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes

  Shuhei Yanase, Tomohisa Hasunuma, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Sep. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 88(1) (1), 381 - 388, English

  [Refereed]

  Scientific journal


• Biofunctional TiO2 nanoparticle-mediated photokilling of cancer cells using UV irradiation

  Kazusa Matsui, Miki Karasaki, Maiko Segawa, Sang Youn Hwang, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Sep. 2010, MEDCHEMCOMM, 1(3) (3), 209 - 211, English

  [Refereed]

  Scientific journal


• Display of both N- and C-terminal target fusion proteins on the Aspergillus oryzae cell surface using a chitin-binding module

  Soichiro Tabuchi, Junji Ito, Takashi Adachi, Hiroki Ishida, Yoji Hata, Fumiyoshi Okazaki, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Aug. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(5) (5), 1783 - 1789, English

  [Refereed]

  Scientific journal


• Co-fermentation of cellobiose and xylose using beta-glucosidase displaying diploid industrial yeast strain OC-2

  Satoshi Saitoh, Tomohisa Hasunuma, Tsutomu Tanaka, Akihiko Kondo

  Aug. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(5) (5), 1975 - 1982, English

  [Refereed]

  Scientific journal


• Surfactant-modified yeast whole-cell biocatalyst displaying lipase on cell surface for enzymatic production of structured lipids in organic media

  Shinji Hama, Ayumi Yoshida, Kazunori Nakashima, Hideo Noda, Hideki Fukuda, Akihiko Kondo

  Jun. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(2) (2), 537 - 543, English

  [Refereed]

  Scientific journal


• Repeated batch fermentation from raw starch using a maltose transporter and amylase expressing diploid yeast strain

  Syun-ichi Yamakawa, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Jun. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(1) (1), 109 - 115, English

  [Refereed]

  Scientific journal


• Importance of asparagine residues at positions 13 and 26 on the amino-terminal domain of human somatostatin receptor subtype-5 in signalling

  Shota Togawa, Jun Ishii, Atsushi Ishikura, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Jun. 2010, JOURNAL OF BIOCHEMISTRY, 147(6) (6), 867 - 873, English

  [Refereed]

  Scientific journal


• Control of signalling properties of human somatostatin receptor subtype-5 by additional signal sequences on its amino-terminus in yeast

  Yusuke Iguchi, Jun Ishii, Hideki Nakayama, Atsushi Ishikura, Keiko Izawa, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo

  Jun. 2010, JOURNAL OF BIOCHEMISTRY, 147(6) (6), 875 - 884, English

  [Refereed]

  Scientific journal


• Ethanol production from cellulosic materials using cellulase-expressing yeast

  Shuhei Yanase, Ryosuke Yamada, Shohei Kaneko, Hideo Noda, Tomohisa Hasunuma, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  May 2010, BIOTECHNOLOGY JOURNAL, 5(5) (5), 449 - 455, English

  [Refereed]

  Scientific journal


• Enhancement of beta-glucosidase activity on the cell-surface of sake yeast by disruption of SED1

  Atsushi Kotaka, Hiroshi Sahara, Kouichi Kuroda, Akihiko Kondo, Mitsuyoshi Ueda, Yoji Hata

  May 2010, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 109(5) (5), 442 - 446, English

  [Refereed]

  Scientific journal


• Cocktail delta-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains

  Ryosuke Yamada, Naho Taniguchi, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  May 2010, MICROBIAL CELL FACTORIES, 9, English

  [Refereed]

  Scientific journal


• Evaluation of whole-abdominal fat volume by 700-slice CT scanning and comparison with the umbilical fat area anthropometric indices

  Kentaro Furukawa, Takuyuki Katabami, Yasuo Nakajima, Tomoko Sato, Hiroyuki Kato, Rieko Koganei, Shiko Asai, Tomoya Matsui, Yukiyoshi Sata, Takehiro Kawata, Akihiko Kondo, Akio Ohta, Yasushi Tanaka

  Apr. 2010, Obesity Research and Clinical Practice, 4(2) (2), e111 - e117, English

  [Refereed]

  Scientific journal


• The competitor-introduced G gamma recruitment system, a new approach for screening affinity-enhanced proteins

  Nobuo Fukuda, Jun Ishii, Tsutomu Tanaka, Akihiko Kondo

  Apr. 2010, FEBS JOURNAL, 277(7) (7), 1704 - 1712, English

  [Refereed]

  Scientific journal


• Selection of DNA aptamers using atomic force microscopy

  Yusuke Miyachi, Nobuaki Shimizu, Chiaki Ogino, Akihiko Kondo

  Mar. 2010, NUCLEIC ACIDS RESEARCH, 38(4) (4), English

  [Refereed]

  Scientific journal


• Metabolic turnover analysis by a combination of in vivo C-13-labelling from (CO2)-C-13 and metabolic profiling with CE-MS/MS reveals rate-limiting steps of the C-3 photosynthetic pathway in Nicotiana tabacum leaves

  Tomohisa Hasunuma, Kazuo Harada, Shin-Ichi Miyazawa, Akihiko Kondo, Eiichiro Fukusaki, Chikahiro Miyake

  Mar. 2010, JOURNAL OF EXPERIMENTAL BOTANY, 61(4) (4), 1041 - 1051, English

  [Refereed]

  Scientific journal


• Fatty acid production from butter using novel cutinase-displaying yeast

  Ken Horii, Takashi Adachi, Takanori Tanino, Tsutomu Tanaka, Atsushi Kotaka, Hiroshi Sahara, Tsuneharu Hashimoto, Nobuyuki Kuratani, Seiji Shibasaki, Chiaki Ogino, Hideo Noda, Yoji Hata, Mitsuyoshi Ueda, Akihiko Kondo

  Mar. 2010, ENZYME AND MICROBIAL TECHNOLOGY, 46(3-4) (3-4), 194 - 199, English

  [Refereed]

  Scientific journal


• Novel strategy for yeast construction using δ-integration and cell fusion to efficiently produce ethanol from raw starch

  Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Feb. 2010, Applied Microbiology and Biotechnology, 85(5) (5), 1491 - 1498, English

  [Refereed]

  Scientific journal


• Reliability of behavioral analyses in a partial lesion rat model of Parkinson's disease in relation to nigrostriatal dopaminergic depletion

  Akihiko Kondo

  2010, Neuroscience Research

  Scientific journal


• Intravenous administration of mesenchymal stem cells exerts therapeutic effects on parkinsonian model of rats: Focusing on neuroprotective effects of stromal cell-derived factor-1α

  Akihiko Kondo

  2010, BMC Neuroscience

  Scientific journal


• Metabolic Engineering by Plastid Transformation as a Strategy to Modulate Isoprenoid Yield in Plants

  Akihiko Kondo

  2010, Methods in Molecular Biology

  Scientific journal


• Exercise exerts neuroprotective effects on Parkinson's disease model of rats

  Akihiko Kondo

  2010, Brain Research

  Scientific journal


• 高効率バイオディーゼル生産を目的としたWhole-cell biocatalystによる新規リパーゼ複合系の開発

  足立 大輔, 中島 一紀, 荻野 千秋, 近藤 昭彦

  公益社団法人 化学工学会, 2010, 化学工学会 研究発表講演要旨集, 2010, 1008 - 1008, Japanese


• 力学的指標による生細胞表面におけるリガンド‐受容体の相互作用解析

  野坂 和輝, 宮地 佑典, 石井 純, 荻野 千秋, 近藤 昭彦

  公益社団法人 化学工学会, 2010, 化学工学会 研究発表講演要旨集, 2010, 983 - 983, Japanese


• affibody提示バイオナノカプセルを用いたタンパク質デリバリーシステム

  西村 勇哉, 三枝 宏彰, 田中 勉, 荻野 千秋, 近藤 昭彦

  公益社団法人 化学工学会, 2010, 化学工学会 研究発表講演要旨集, 2010, 963 - 963, Japanese


• ガラクトース転写誘導系を利用した酵母リガンド検出系の開発

  漁 慎太郎, 井口 裕介, 福田 展雄, 石井 純, 近藤 昭彦

  公益社団法人 化学工学会, 2010, 化学工学会 研究発表講演要旨集, 2010, 977 - 977, Japanese


• Production of biofuels and chemicals from biomass (Biorefinery): recent achievement and perspective

  Kondo Akihiko

  バイオマスからの燃料・化成品生産（バイオリファイナリー）の現状と展望について解説する。

  The Japan Petroleum Institute, 2010, Proceeding of Annual/Fall Meetings of the Japan Petroleum Institute, 2010, 1 - 1, Japanese


• Intensification of bio-ethanol fermentation by recombinant yeast with xylose isomerase pathway

  Tomonori Ito, Atsushi Hotta, Atsuko Uchida, Takanori Tanino, Chiaki Ogino, Akihiko Kondo, Naoto Ohmura

  Italian Association of Chemical Engineering - AIDIC, 2010, Chemical Engineering Transactions, 20, 103 - 108, English

  [Refereed]

  International conference proceedings


• Relationship between clinical markers of glycemia and glucose excursion evaluated by Continuous Glucose Monitoring (CGM)

  Tomoko Suwa, Akio Ohta, Tomoya Matsui, Rieko Koganei, Hiroyuki Kato, Takehiro Kawata, Yukiyoshi Sada, Satoshi Ishii, Akihiko Kondo, Kaori Murakami, Takuyuki Katabami, Yasushi Tanaka

  2010, Endocrine Journal, 57(2) (2), 135 - 140, English

  [Refereed]

  Scientific journal


• Intensification of Bio-ethanol Fermentation by Recombinant Yeast with Xylose Isomerase Pathway

  Tomonori Ito, Atsushi Hotta, Atsuko Uchida, Takanori Tanino, Chiaki Ogino, Akihiko Kondo, Naoto Ohmura

  2010, IBIC2010: 2ND INTERNATIONAL CONFERENCE ON INDUSTRIAL BIOTECHNOLOGY, 20, 103 - 108, English

  [Refereed]

  International conference proceedings


• Production of biodiesel fuel from soybean oil catalyzed by fungus whole-cell biocatalysts in ionic liquids

  Shogo Arai, Kazunori Nakashima, Takanori Tanino, Chiaki Ogino, Akihiko Kondo, Hideki Fukuda

  Jan. 2010, ENZYME AND MICROBIAL TECHNOLOGY, 46(1) (1), 51 - 55, English

  [Refereed]

  Scientific journal


• D-lactic acid production from cellooligosaccharides and beta-glucan using L-LDH gene-deficient and endoglucanase-secreting Lactobacillus plantarum

  Kenji Okano, Qiao Zhang, Shogo Yoshida, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Jan. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85(3) (3), 643 - 650, English

  [Refereed]

  Scientific journal


• Displaying non-natural, functional molecules on yeast surfaces via biotin-streptavidin interaction

  Tsutomu Tanaka, Shinsuke Masunari, Jun Ishii, Kanako Wakamura, Maiko Segawa, Hideki Fukuda, Akihiko Kondo

  Jan. 2010, JOURNAL OF BIOTECHNOLOGY, 145(1) (1), 79 - 83, English

  [Refereed]

  Scientific journal


• Biotechnological production of enantiomeric pure lactic acid from renewable resources: recent achievements, perspectives, and limits

  Kenji Okano, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Jan. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85(3) (3), 413 - 423, English

  [Refereed]

  Scientific journal


• Improved Production of Homo-D-Lactic Acid via Xylose Fermentation by Introduction of Xylose Assimilation Genes and Redirection of the Phosphoketolase Pathway to the Pentose Phosphate Pathway in L-Lactate Dehydrogenase Gene-Deficient Lactobacillus plantarum

  Kenji Okano, Shogo Yoshida, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Dec. 2009, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(24) (24), 7858 - 7861, English

  [Refereed]

  Scientific journal


• Evaluation of the Biodegradability of Polyurethane and Its Derivatives by Using Lipase-Displaying Arming Yeast

  Seiji Shibasaki, Al Kawabata, Takanori Tanino, Akihiko Kondo, Mitsuyoshi Ueda, Mamoru Tanaka

  Dec. 2009, BIOCONTROL SCIENCE, 14(4) (4), 171 - 175, English

  [Refereed]

  Scientific journal


• Biotinylated Bionanocapsules for Displaying Diverse Ligands Toward Cell-specific Delivery

  Takuya Shishido, Yuki Azumi, Takeshi Nakanishi, Mitsuo Umetsu, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Dec. 2009, JOURNAL OF BIOCHEMISTRY, 146(6) (6), 867 - 874, English

  [Refereed]

  Scientific journal


• Split-ubiquitin system for analyzing oligomerization of G protein-coupled receptor

  Yasuyuki Nakamura, Jun Ishii, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(1) (1), S36 - S36, English

  [Refereed]

  Scientific journal


• Using promoter replacement and selection for loss of heterozygosity to generate an industrially applicable sake yeast strain that homozygously overproduces isoamyl acetate

  Hiroshi Sahara, Atsushi Kotaka, Akihiko Kondo, Mitsuyoshi Ueda, Yoji Hata

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(5) (5), 359 - 364, English

  [Refereed]

  Scientific journal


• Ester synthesis reaction with CALB displaying yeast whole cell biocatalyst: Effect of organic solvent and initial water content

  Takanori Tanino, Takumi Ohm, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(5) (5), 369 - 371, English

  [Refereed]

  Scientific journal


• Dimensionality reduction for metabolome data using PCA, PLS, OPLS, and RFDA with differential penalties to latent variables

  Hiroyuki Yamamoto, Hideki Yamaji, Yuichiro Abe, Kazuo Harada, Danang Waluyo, Eiichiro Fukusaki, Akihiko Kondo, Hiromu Ohno, Hideki Fukuda

  Oct. 2009, Chemometrics and Intelligent Laboratory Systems, 98(2) (2), 136 - 142, English

  [Refereed]

  Scientific journal


• Overproduction of Geranylgeraniol by Metabolically Engineered Saccharomyces cerevisiae

  Kenro Tokuhiro, Masayoshi Muramatsu, Chikara Ohto, Toshiya Kawaguchi, Shusei Obata, Nobuhiko Muramoto, Masana Hirai, Haruo Takahashi, Akihiko Kondo, Eiji Sakuradani, Sakayu Shimizu

  Sep. 2009, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(17) (17), 5536 - 5543, English

  [Refereed]

  Scientific journal


• Development of novel cell surface display in Corynebacterium glutamicum using porin

  Toshihiro Tateno, Kazuki Hatada, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  Sep. 2009, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 84(4) (4), 733 - 739, English

  [Refereed]

  Scientific journal


• Improvement of isoflavone aglycones production using beta-glucosidase secretory produced in recombinant Aspergillus oryzae

  Ken Horii, Takashi Adachi, Tetsuya Matsuda, Tsutomu Tanaka, Hiroshi Sahara, Seiji Shibasaki, Chiaki Ogino, Yoji Hata, Mitsuyoshi Ueda, Akihiko Kondo

  Aug. 2009, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 59(4) (4), 297 - 301, English

  [Refereed]

  Scientific journal


• Homo-D-Lactic Acid Fermentation from Arabinose by Redirection of the Phosphoketolase Pathway to the Pentose Phosphate Pathway in L-Lactate Dehydrogenase Gene-Deficient Lactobacillus plantarum

  Kenji Okano, Shogo Yoshida, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Aug. 2009, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(15) (15), 5175 - 5178, English

  [Refereed]

  Scientific journal


• Evaluation of cell surface-displayed protein stability against simulated gastric fluid

  Ken Horii, Takashi Adachi, Takanori Tanino, Tsutomu Tanaka, Hiroshi Sahara, Seiji Shibasaki, Chiaki Ogino, Yoji Hata, Mitsuyoshi Ueda, Akihiko Kondo

  Aug. 2009, BIOTECHNOLOGY LETTERS, 31(8) (8), 1259 - 1264, English

  [Refereed]

  Scientific journal


• Development of an enzyme activity screening system for beta-glucosidase-displaying yeasts using calcium alginate micro-beads and flow sorting

  Yan Zhou, Shin'ichiro Kajiyama, Kouichi Itoh, Takanori Tanino, Nobuo Fukuda, Tsutomu Tanaka, Akihiko Kondo, Kiichi Fukui

  Aug. 2009, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 84(2) (2), 375 - 382, English

  [Refereed]

  Scientific journal


• Selection of a DNA aptamer that binds 8-OHdG using GMP-agarose

  Yusuke Miyachi, Nobuaki Shimizu, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Jul. 2009, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 19(13) (13), 3619 - 3622, English

  [Refereed]

  Scientific journal


• Use of mono- and diacylglycerol lipase as immobilized fungal whole cells to convert residual partial glycerides enzymatically into fatty acid methyl esters

  Shinji Hama, Takao Numata, Sriappareddy Tamalampudi, Ayumi Yoshida, Hideo Noda, Akihiko Kondo, Hideki Fukuda

  Jun. 2009, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 58(1-4) (1-4), 93 - 97, English

  [Refereed]

  Scientific journal


• Regulation of the Display Ratio of Enzymes on the Saccharomyces cerevisiae Cell Surface by the Immunoglobulin G and Cellulosomal Enzyme Binding Domains

  Junji Ito, Akihiko Kosugi, Tsutomu Tanaka, Kouichi Kuroda, Seiji Shibasaki, Chiaki Ogino, Mitsuyoshi Ueda, Hideki Fukuda, Roy H. Doi, Akihiko Kondo

  Jun. 2009, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(12) (12), 4149 - 4154, English

  [Refereed]

  Scientific journal


• Marker-disruptive gene integration and URA3 recycling for multiple gene manipulation in Saccharomyces cerevisiae

  Shohei Kaneko, Tsutomu Tanaka, Hideo Noda, Hideki Fukuda, Rinji Akada, Akihiko Kondo

  Jun. 2009, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 83(4) (4), 783 - 789, English

  [Refereed]

  Scientific journal


• A Simple and Immediate Method for Simultaneously Evaluating Expression Level and Plasmid Maintenance in Yeast

  Jun Ishii, Keiko Izawa, Shizuka Matsumura, Kanako Wakamura, Takanori Tanino, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Jun. 2009, JOURNAL OF BIOCHEMISTRY, 145(6) (6), 701 - 708, English

  [Refereed]

  Scientific journal


• Production of ethanol from cassava pulp via fermentation with a surface-engineered yeast strain displaying glucoamylase

  Akihiko Kosugi, Akihiko Kondo, Mitsuyoshi Ueda, Yoshinori Murata, Pilanee Vaithanomsat, Warunee Thanapase, Takamitsu Arai, Yutaka Mori

  May 2009, RENEWABLE ENERGY, 34(5) (5), 1354 - 1358, English

  [Refereed]

  Scientific journal


• Efficient production of ethanol from raw starch by a mated diploid Saccharomyces cerevisiae with integrated alpha-amylase and glucoamylase genes

  Ryosuke Yamada, Yohei Bito, Takashi Adachi, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  May 2009, ENZYME AND MICROBIAL TECHNOLOGY, 44(5) (5), 344 - 349, English

  [Refereed]

  Scientific journal


• Construction of a novel detection system for protein-protein interactions using yeast G-protein signaling

  Nobuo Fukuda, Jun Ishii, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  May 2009, FEBS Journal, 276(9) (9), 2636 - 2644, English

  [Refereed]

  Scientific journal


• Application of magnetic nanoparticles in full-automated chemiluminescent enzyme immunoassay

  Xiaomao Xie, Noriyuki Ohnishi, Yuki Takahashi, Akihiko Kondo

  May 2009, JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 321(10) (10), 1686 - 1688, English

  [Refereed]

  Scientific journal


• A high-level expression vector containing selectable marker for continuous production of recombinant protein in insect cells

  Takuya Shishido, Naoya Kurata, Myung Eui Yoon, Tsutomu Tanaka, Hideki Yamaji, Hideki Fukuda, Akihiko Kondo

  May 2009, BIOTECHNOLOGY LETTERS, 31(5) (5), 623 - 627, English

  [Refereed]

  Scientific journal


• Xylose isomerase from polycentric fungus Orpinomyces: gene sequencing, cloning, and expression in Saccharomyces cerevisiae for bioconversion of xylose to ethanol

  Anjali Madhavan, Sriappareddy Tamalampudi, Kazunari Ushida, Daisuke Kanai, Satoshi Katahira, Aradhana Srivastava, Hideki Fukuda, Virendra S. Bisaria, Akihiko Kondo

  Apr. 2009, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 82(6) (6), 1067 - 1078, English

  [Refereed]

  Scientific journal


• Double mutation of the PDC1 and ADH1 genes improves lactate production in the yeast Saccharomyces cerevisiae expressing the bovine lactate dehydrogenase gene

  Kenro Tokuhiro, Nobuhiro Ishida, Eiji Nagamori, Satoshi Saitoh, Toru Onishi, Akihiko Kondo, Haruo Takahashi

  Apr. 2009, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 82(5) (5), 883 - 890, English

  [Refereed]

  Scientific journal


• Bioenergy: Sustainable fuels from biomass by yeast and fungal whole-cell biocatalysts

  Hideki Fukuda, Akihiko Kondo, Sriappareddy Tamalampudi

  Apr. 2009, BIOCHEMICAL ENGINEERING JOURNAL, 44(1) (1), 2 - 12, English

  [Refereed]

  Scientific journal


• Alcoholic fermentation of xylose and mixed sugars using recombinant Saccharomyces cerevisiae engineered for xylose utilization

  Anjali Madhavan, Sriappareddy Tamalampudi, Aradhana Srivastava, Hideki Fukuda, Virendra S. Bisaria, Akihiko Kondo

  Apr. 2009, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 82(6) (6), 1037 - 1047, English

  [Refereed]

  Scientific journal


• Metabolic pathway engineering by plastid transformation is a powerful tool for production of compounds in higher plants

  Tomohisa Hasunuma, Akihiko Kondo, Chikahiro Miyake

  Mar. 2009, PLANT BIOTECHNOLOGY, 26(1) (1), 39 - 46, English

  [Refereed]

  Scientific journal


• Production of ethanol from cassava pulpvia fermentation with a surface-engineered yeast strain displaying glucoamylase.

  KOSUGI A, KONDO Akihiko, UEDA M, MURATA Y, VAITHANOMSAT P, THANAPASE W. ARAI T, MORI Y

  Mar. 2009, Renewable Energy, Vol. 34, No. 5, pp. 1354-1358, English

  [Refereed]

  Scientific journal


• Construction of arginine-rich peptide displaying bionanocapsules

  Takuya Shishido, Daisaku Yonezawa, Kiyokazu Iwata, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Mar. 2009, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 19(5) (5), 1473 - 1476, English

  [Refereed]

  Scientific journal


• Improvement of a Candida antarctica lipase B-displaying yeast whole-cell biocatalyst and its application to the polyester synthesis reaction

  Takanori Tanino, Tohru Aoki, Won-young Chung, Yuka Watanabe, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Feb. 2009, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 82(1) (1), 59 - 66, English

  [Refereed]

  Scientific journal


• Efficient generation of recessive traits in diploid sake yeast by targeted gene disruption and loss of heterozygosity

  Atsushi Kotaka, Hiroshi Sahara, Akihiko Kondo, Mitsuyoshi Ueda, Yoji Hata

  Feb. 2009, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 82(2) (2), 387 - 395, English

  [Refereed]

  Scientific journal


• Direct production of cadaverine from soluble starch using Corynebacterium glutamicum coexpressing alpha-amylase and lysine decarboxylase

  Toshihiro Tateno, Yusuke Okada, Takeyuki Tsuchidate, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  Feb. 2009, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 82(1) (1), 115 - 121, English

  [Refereed]

  Scientific journal


• The combined therapy of intrahippocampal transplantation of adult neural stem cells and intraventricular erythropoietin-infusion ameliorates spontaneous recurrent seizures by suppression of abnormal mossy fiber sprouting

  Akihiko Kondo

  2009, Brain Research

  Scientific journal


• SELECTION OF DNA APTAMERS USING ATOMIC FORCE MICROSCOPY

  Akihiko Kondo

  2009, IFPT'6: PROGRESS ON POST-GENOME TECHNOLOGIES, PROCEEDINGS

  Scientific journal


• Production of biofuels and chemicals by using cell surface and metabolic pathway engineered microbial cells

  Akihiko Kondo

  2009, Journal of Bioscience and Bioengineering

  Scientific journal


• Injection of muscimol, a GABAa agonist into the anterior thalamic nucleus, suppresses hippocampal neurogenesis in amygdala-kindled rats

  Akihiko Kondo

  2009, Neurological Research

  Scientific journal


• Erythropoietin exerts anti-epileptic effects with the suppression of aberrant new cell formation in the dentate gyrus and upregulation of neuropeptide Y in seizure model of rats

  Akihiko Kondo

  2009, Brain Research

  Scientific journal


• Electrical Stimulation of the Cerebral Cortex Exerts Antiapoptotic, Angiogenic, and Anti-Inflammatory Effects in Ischemic Stroke Rats Through Phosphoinositide 3-Kinase/Akt Signaling Pathway

  Akihiko Kondo

  2009, Stroke

  Scientific journal


• Continuous intraventricular infusion of erythropoietin exerts neuroprotective/rescue effects upon Parkinson's disease model of rats with enhanced neurogenesis

  Akihiko Kondo

  2009, Brain Research

  Scientific journal


• キシロースからの効率的なエタノール生産を行う酵母の創製

  加藤 寛子, 山田 亮祐, 蓮沼 誠久, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2009, 化学工学会 研究発表講演要旨集, 2009, 4 - 4, Japanese


• [依頼講演]酵素法によるバイオディーゼル燃料の生産プラント

  浜 真司, 倉谷 伸行, Tamalampudi Sriappareddy, 近藤 昭彦, 野田 秀夫, 福田 秀樹

  公益社団法人 化学工学会, 2009, 化学工学会 研究発表講演要旨集, 2009, 769 - 769, Japanese


• 非侵襲性がん治療のためのタンパク質修飾ナノ粒子の開発

  松井 かずさ, 荻野 千秋, 田中 勉, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2009, 化学工学会 研究発表講演要旨集, 2009, 727 - 727, Japanese


• 酵素法によるバイオディーゼル生産を目的としたグリセリン分離型反応装置の開発

  ?? 真司, 玉谷 直紀, 吉田 あゆみ, 倉谷 伸行, 近藤 昭彦, 福田 秀樹, 野田 秀夫

  公益社団法人 化学工学会, 2009, 化学工学会 研究発表講演要旨集, 2009, 675 - 675, Japanese


• タンパク質連結酵素を用いた酵素融合タンパク質調製法の開発

  澤本 詩織, 坂本 祟幸, 田中 勉, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2009, 化学工学会 研究発表講演要旨集, 2009, 631 - 631, Japanese


• Bioethanol Production from Cellulose Material by Arming Yeast(New Midori Revolution-Seeking after a dream energy-)

  Ogino Chiaki, Kondo Akihiko

  平成14年度に日本政府の総合戦略「バイオマス・ニッポン」が策定され、バイオマスの有効利用による持続的に発展可能な社会の実現が提言されている。この戦略は、（1）バイオマスの有効利用に基づく地球温暖化防止や循環型社会形成の達成、（2）日本独自のバイオマス利用法の開発による戦略的産業の育成を目指すものである。また、地球規模での環境保護の観点から、バイオマス原料は日本のみならず、世界中から安価かつ豊富な資源の積極的な利用が求められている。さらに石油資源枯渇や価格高騰の影響より、これまでの石油資源依存型の「オイルリファイナリー」から、バイオマスベースの「バイオリファイナリー」社会への転換が急務とされている。米国においても、1999年にクリントン大統領が「バイオ製品およびバイオマスエネルギーの開発・普及に関する大統領令」を発令し、バイオマスエネルギーの利用率を2010年までに10％までに拡大する方針を指示し、ヨーロッパにおいても同様の方針が打ち出されている状況である。このような社会的背景も重なり、現在、グルコースから生産されるバイオエタノールはブラジル、米国をはじめとして多くの地域でガソリンと混合され、自動車用燃料として利用されている。しかしながら、食料価格高騰の問題原因ともなっており、代替原料が模索されている。木質系バイオマスはデンプンよりもはるかに豊富に存在し、また古紙や廃材、農産廃棄物など処理が問題化しているものを利用するため原料が安価であることなどから、将来の石油代替エネルギー資源として特に有望視されている。

  Japanese Society of Grassland Science, 2009, Japanese Journal of Grassland Science, 55(3) (3), 270 - 273, Japanese


• 菌体触媒を用いたバイオディーゼル生産におけるイオン液体の利用

  中島 一紀, 荒井 章吾, 谷野 孝徳, 荻野 千秋, 近藤 昭彦, 福田 秀樹

  公益社団法人 化学工学会, 2009, 化学工学会 研究発表講演要旨集, 2009, 912 - 912, Japanese


• Delivery of gene-expressing fragments using quantum dot

  Akiyoshi Hoshino, Noriyoshi Manabe, Sanshiro Hanada, Kouki Fujioka, Masato Yasuhara, Kondo Akihiko, Kenji Yamamoto

  2009, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 7189, English

  [Refereed]

  International conference proceedings


• CONSTRUCTION OF A NOVEL DETECTION SYSTEM FOR PROTEIN-PROTEIN INTERACTIONS USING YEAST G-PROTEIN SIGNALING

  Nobuo Fukuda, Jun Ishii, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  2009, IFPT'6: PROGRESS ON POST-GENOME TECHNOLOGIES, PROCEEDINGS, 238 - 238, English

  [Refereed]

  International conference proceedings


• Evaluation of the biodegradability of polyurethane and its derivatives by using lipase-displaying arming yeast

  Seiji Shibasaki, Al Kawabata, Takanori Tanino, Akihiko Kondo, Mitsuyoshi Ueda, Mamoru Tanaka

  2009, Biocontrol Science, 14(4) (4), 171 - 175, English

  [Refereed]

  Scientific journal


• Efficient Production of Optically Pure D-Lactic Acid from Raw Corn Starch by Using a Genetically Modified L-Lactate Dehydrogenase Gene-Deficient and alpha-Amylase-Secreting Lactobacillus plantarum Strain

  Kenji Okano, Qiao Zhang, Satoru Shinkawa, Shogo Yoshida, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  Jan. 2009, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(2) (2), 462 - 467, English

  [Refereed]

  Scientific journal


• Specific Protein Delivery to Target Cells by Antibody-displaying Bionanocapsules

  Naoya Kurata, Takuya Shishido, Masaru Muraoka, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Dec. 2008, JOURNAL OF BIOCHEMISTRY, 144(6) (6), 701 - 707, English

  [Refereed]

  Scientific journal


• Preparation and comparative characterization of immobilized Aspergillus oryzae expressing Fusarium heterosporum lipase for enzymatic biodiesel production

  Shinji Hama, Sriappareddy Tamalampudi, Yuya Suzuki, Ayumi Yoshida, Hideki Fukuda, Akihiko Kondo

  Dec. 2008, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 81(4) (4), 637 - 645, English

  [Refereed]

  Scientific journal


• GFP expression by intracellular gene delivery of GFP-coding fragments using nanocrystal quantum dots

  Akiyoshi Hoshino, Noriyoshi Manabe, Kouki Fujioka, Sanshiro Hanada, Masato Yasuhara, Akihiko Kondo, Kenji Yamamoto

  Dec. 2008, NANOTECHNOLOGY, 19(49) (49), English

  [Refereed]

  Scientific journal


• Construction of an Aspergillus oryzae cell-surface display system using a putative GPI-anchored protein

  Takashi Adachi, Junji Ito, Kouji Kawata, Masahiro Kaya, Hiroki Ishida, Hiroshi Sahara, Yoji Hata, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Dec. 2008, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 81(4) (4), 711 - 719, English

  [Refereed]

  Scientific journal


• Breeding of Industrial Diploid Yeast Strain with Chromosomal Integration of Multiple beta-Glucosidase Genes

  Satoshi Saitoh, Tsutomu Tanaka, Akihiko Kondo

  Dec. 2008, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 106(6) (6), 594 - 597, English

  [Refereed]

  Scientific journal


• Effective xylose/cellobiose co-fermentation and ethanol production by xylose-assimilating S-cerevisiae via expression of beta-glucosidase on its cell surface

  Nobuhiro Nakamura, Ryosuke Yamada, Satoshi Katahira, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  Sep. 2008, ENZYME AND MICROBIAL TECHNOLOGY, 43(3) (3), 233 - 236, English

  [Refereed]

  Scientific journal


• Development of a yeast protein fragment complementation assay (PCA) system using dihydrofolate reductase (DHFR) with specific additives

  Seiji Shibasaki, Kozue Sakata, Jun Ishii, Akihiko Kondo, Mitsuyoshi Ueda

  Sep. 2008, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 80(4) (4), 735 - 743, English

  [Refereed]

  Scientific journal


• Characterization of yeast cell surface displayed Aspergillus oryzae beta-glucosidase 1 high hydrolytic activity for soybean isoflavone

  Junji Ito, Hiroshi Sahara, Masahiko Kaya, Yoji Hata, Seiji Shibasaki, Koji Kawata, Saori Ishida, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Sep. 2008, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 55(1-2) (1-2), 69 - 75, English

  [Refereed]

  Scientific journal


• Improvement of ethanol productivity during xylose and glucose co-fermentation by xylose-assimilating S. cerevisiae via expression of glucose transporter Sut1

  Satoshi Katahira, Meguru Ito, Hisae Takema, Yasuya Fujita, Takanori Tanino, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  Aug. 2008, ENZYME AND MICROBIAL TECHNOLOGY, 43(2) (2), 115 - 119, English

  [Refereed]

  Scientific journal


• Role of N-terminal 28-amino-acid region of Rhizopus oryzae lipase in directing proteins to secretory pathway of Aspergillus oryzae

  Shinji Hama, Sriappareddy Tamalampudi, Naoki Shindo, Takao Numata, Hideki Yamaji, Hideki Fukuda, Akihiko Kondo

  Jul. 2008, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 79(6) (6), 1009 - 1018, English

  [Refereed]

  Scientific journal


• Lactic fermentation of cellobiose by a yeast strain displaying beta-glucosidase on the cell surface

  Kenro Tokuhiro, Nobuhiro Ishida, Akihiko Kondo, Haruo Takahashi

  Jun. 2008, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 79(3) (3), 481 - 488, English

  [Refereed]

  Scientific journal


• Direct ethanol production from barley beta-glucan by sake yeast displaying Aspergillus oryzae beta-glucosidase and endoglucanase

  Atsushi Kotaka, Hiroki Bando, Masahiko Kaya, Michiko Kato-Murai, Kouichi Kuroda, Hiroshi Sahara, Yoji Hata, Akihiko Kondo, Mitsuyoshi Ueda

  Jun. 2008, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 105(6) (6), 622 - 627, English

  [Refereed]

  Scientific journal


• Yeast-based fluorescence reporter assay of G protein-coupled receptor signaling for flow cytometric screening: FAR1-disruption recovers loss of episomal plasmid caused by signaling in yeast

  Jun Ishii, Tsutomu Tanaka, Shizuka Matsumura, Kenji Tatematsu, Shunichi Kuroda, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Here, we describe a yeast-based fluorescence reporter assay for G protein-coupled receptor (GPCR) signalling using a flow cytometer (FCM). The enhanced green fluorescent protein (EGFP) gene was integrated into the FUS1 locus as a reporter gene. The engineered yeast was able to express the EGFP in response to ligand stimulation. Gene-disrupted yeast strains were constructed to evaluate the suitability of the yeast-based fluorescence screening system for heterologous GPCR. When receptor was expressed by episomal plasmid, the proportion of the signalling-activated cells in response to ligand stimulation decreased significantly. The GPCR-signalling-activated and non-activated cell clusters were individually isolated by analysing the fluorescence intensity at the single-cell level with FCM, and it was found that the plasmid retention rate decays markedly in the non-activated cell cluster. We attributed the loss of plasmid to G1 arrest in response to signalling, and successfully improved the plasmid retention rate by disrupting the FAR1 gene and avoiding cell cycle arrest. Our system will be a powerful tool for the quantitative and high-throughput GPCR screening of yeast-based combinatorial libraries using FCM.

  May 2008, Journal of Biochemistry, Vol 143, pp. 667-674(5) (5), 667 - 74, English, International magazine

  [Refereed]

  Scientific journal


• Isoflavone aglycones production from isoflavone glycosides by display of beta-glucosidase from Aspergillus oryzae on yeast cell surface

  Masahiko Kaya, Junji Ito, Atsushi Kotaka, Kengo Matsumura, Hiroki Bando, Hiroshi Sahara, Chiaki Ogino, Seiji Shibasaki, Kouichi Kuroda, Mitsuyoshi Ueda, Akihiko Kondo, Yoji Hata

  May 2008, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 79(1) (1), 51 - 60, English

  [Refereed]

  Scientific journal


• Efficient and direct fermentation of starch to ethanol by sake yeast strains displaying fungal glucoamylases

  Atsushi Kotaka, Hiroshi Sahara, Yoji Hata, Yasuhisa Abe, Akihiko Kondo, Michiko Kato-Murai, Kouichi Kuroda, Mitsuyoshi Ueda

  May 2008, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 72(5) (5), 1376 - 1379, English

  [Refereed]

  Scientific journal


• Rapid and efficient selection of yeast displaying a target protein using thermo-responsive magnetic nanoparticles

  Akihiko Kondo

  Apr. 2008, Biotechnology Progress, Vol. 24, No. 2, pp. 352-357, English

  [Refereed]

  Scientific journal


• Enzymatic production of biodiesel from Jatropha oil: A comparative study of immobilized-whole cell and commercial lipases as a biocatalyst

  Sriappareddy Tamalampudi, Mahabubur Rahman Talukder, Shinji Hama, Takao Numata, Akihiko Kondo, Hideki Fukuda

  Apr. 2008, BIOCHEMICAL ENGINEERING JOURNAL, 39(1) (1), 185 - 189, English

  [Refereed]

  Scientific journal


• System using tandem repeats of the cA peptidoglycan-binding domain from Lactococcus lactis for display of both N- and C-terminal fusions on cell surfaces of lactic acid bacteria

  Kenji Okano, Qiao Zhang, Sakurako Kimura, Junya Narita, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

  Feb. 2008, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74(4) (4), 1117 - 1123, English

  [Refereed]

  Scientific journal


• Intrapallidal metabotropic glutamate receptor activation in a rat model of Parkinson's disease: Behavioral and histological analyses

  Akihiko Kondo

  2008, Brain Research

  Scientific journal


• ペプチド転移酵素を用いた細胞内タンパク質連結技術の開発

  若村 香菜子, 田中 勉, 石井 純, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 944 - 944, Japanese


• 酵母蛍光レポーターアッセイによるヒトソマトスタチンレセプター変異体の機能解析

  戸川 翔太, 石井 純, 田中 勉, 立松 健司, 黒田 俊一, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 971 - 971, Japanese


• 酵母でのヒトG蛋白質共役型受容体発現に関する分泌シグナル配列の影響

  井口 裕介, 石井 純, 田中 勉, 立松 健司, 黒田 俊一, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 970 - 970, Japanese


• セルラーゼ発現酵母によるセルロースからのバイオエタノール生産

  柳瀬 修平, 山田 亮祐, 田中 勉, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 990 - 990, Japanese


• 生デンプンからの高効率D-乳酸生産技術の開発

  新川 智, 岡野 憲司, 張 イ肖, 田中 勉, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 988 - 988, Japanese


• Whole-cell biocatalystを使用した菜種油からのバイオディーゼル生産

  甲田 梨沙, 田中 勉, 近藤 昭彦, 福田 秀樹

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 997 - 997, Japanese


• 部分グリセリドリパーゼを用いたバイオディーゼル燃料生産系の開発

  甲田 梨沙, 沼田 崇男, 田中 勉, 近藤 昭彦, 福田 秀樹

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 399 - 399, Japanese


• C5・C6糖からの効率的なエタノール生産技術の開発

  阿部 祐一郎, 山田 亮介, 中村 延博, 田中 勉, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 394 - 394, Japanese


• フローサイトメーター(FCM)を利用した酵母GPCRシグナリング解析技術

  石井 純, 田中 勉, 松村 静香, 立松 健司, 黒田 俊一, 荻野 千秋, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 348 - 348, Japanese


• 抗体提示バイオナノカプセルを用いたピンポイントプロテインデリバリーシステムの開発

  安住 友希, 倉田 直弥, 宍戸 卓矢, 上田 政和, 妹尾 昌治, 多田 宏子, 谷澤 克行, 黒田 俊一, 田中 勉, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2008, 化学工学会 研究発表講演要旨集, 2008, 320 - 320, Japanese


• Direct production of L-lysine from raw corn starch by Corynebacterium glutamicum secreting Streptococcus bovis alpha-amylase using cspB promoter and signal sequence

  Toshihiro Tateno, Hideki Fukuda, Akihiko Kondo

  Dec. 2007, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 77(3) (3), 533 - 541, English

  [Refereed]

  Scientific journal


• Separation of murine neutrophils and macrophages by thermoresponsive magnetic nanoparticles

  Akiyoshi Hoshino, Noriyuki Ohnishi, Masato Yasuhara, Kenji Yamamoto, Akihiko Kondo

  Nov. 2007, BIOTECHNOLOGY PROGRESS, 23(6) (6), 1513 - 1516, English

  [Refereed]

  Scientific journal


• Production of alkyl glucoside from cellooligosaccharides using yeast strains displaying Aspergillus aculeatus beta-glucosidase 1

  Junji Ito, Takumi Ebe, Seiji Shibasaki, Hideki Fukuda, Akihiko Kondo

  Nov. 2007, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 49(1-4) (1-4), 92 - 97, English

  [Refereed]

  Scientific journal


• Determination and Analysis of the Starch Binding Domain of Streptococcus bovis 148 Raw-Starch-Hydrolyzing α-Amylase

  MATSUI Yoshimitsu, OKADA Sanae, UCHIMURA Tai, KONDO Akihiko, SATOH Eiichi

  We analyzed the functional structure of Streptococcus bovis 148 α-amylase (AmyA) to determine the starch binding domain (SBD) of this enzyme. Several derivative AmyAs and putative SBD segments were constructed and assayed their adsorption ability onto raw starch. On the basis of the results, a putative linker domain region and two SBDs (SBDα and SBDβ) were found at the C-terminal region of AmyA. SBDα and SBDβ showed 56% amino acid sequence similarity; these SBDs were considered to be tandem repeat motif. In addition, we constructed a chimeric enzyme CsaB, which consisted of S. bovis 148 irresolvable intracellular α-amylase (AmyB) as for insoluble starch and a putative linker plus two SBDs. This enzyme succeeded in conferring starch adsorption and hydrolysis abilities, which suggests that SBD reacts with the surface of raw starch and breaks down its structure.

  The Japanese Society of Applied Glycoscience, Oct. 2007, Journal of Applied Glycoscience, 54(4) (4), 217 - 222, English


• Immobilized recombinant Aspergillus oryzae expressing heterologous lipase: An efficient whole-cell biocatalyst for enantioselective transesterification in non-aqueous medium

  Sriappareddy Tamalampudi, Shinji Hama, Takanori Tanino, Mahabubur Rahman Talukder, Akihiko Kondo, Hideki Fukuda

  Sep. 2007, Journal of Molecular Catalysis B: Enzymatic, 48(1-2) (1-2), 33 - 37, English

  [Refereed]

  Scientific journal


• High-efficiency recovery of target cells using improved yeast display system for detection of protein-protein interactions

  Nobuo Fukuda, Jun Ishii, Seiji Shibasaki, Mitsuyoshi Ueda, Hideki Fukuda, Akihiko Kondo

  Aug. 2007, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 76(1) (1), 151 - 158, English

  [Refereed]

  Scientific journal


• Development of yeast cells displaying Candida antarctica lipase B and their application to ester synthesis reaction

  Takanori Tanino, Takumi Ohno, Tohru Aoki, Hideki Fukuda, Akihiko Kondo

  Jul. 2007, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 75(6) (6), 1319 - 1325, English

  [Refereed]

  Scientific journal


• Improvement in lactic acid production from starch using alpha-amylase-secreting Lactococcus lactis cells adapted to maltose or starch

  Kenji Okano, Sakurako Kimura, Junya Narita, Hideki Fukuda, Akihiko Kondo

  Jul. 2007, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 75(5) (5), 1007 - 1013, English

  [Refereed]

  Scientific journal


• Continuous production of phospholipase D using immobilized recombinant Streptomyces lividans

  Chiaki Ogino, Masayuki Kanemasu, Masashi Fukumoto, Takafumi Kubo, Takamichi Yoshino, Akihiko Kondo, Hideki Fukuda, Nobuaki Shimizu

  Jul. 2007, ENZYME AND MICROBIAL TECHNOLOGY, 41(1-2) (1-2), 156 - 161, English

  [Refereed]

  Scientific journal


• Production of bionanocapsules in immobilized insect cell culture using porous biomass support particles

  Takuya Shishido, Masaru Muraoka, Hideki Yamaji, Akihiko Kondo, Hideki Fukuda

  Jun. 2007, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 103(6) (6), 572 - 574, English

  [Refereed]

  Scientific journal


• Biodiesel-fuel production in a packed-bed reactor using lipase-producing Rhizopus oryzae cells immobilized within biomass support particles

  Shinji Hama, Hideki Yamaji, Takahiro Fukumizu, Takao Numata, Sriappareddy Tamalampudi, Akihiko Kondo, Hideo Noda, Hideki Fukuda

  Jun. 2007, BIOCHEMICAL ENGINEERING JOURNAL, 34(3) (3), 273 - 278, English

  [Refereed]

  Scientific journal


• Preparation of a whole-cell biocatalyst of mutated Candida antaretica lipase B (mCALB) by a yeast molecular display system and its practical properties

  Michiko Kato, Jun Fuchimoto, Takanori Tanino, Akihiko Kondo, Hideki Fukuda, Mitsuyoshi Ueda

  Jun. 2007, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 75(3) (3), 549 - 555, English

  [Refereed]

  Scientific journal


• Remarkable enhancement in PLD activity from Streptoverticillium cinnamoneum by substituting serine residue into the GG/GS motif

  Chiaki Ogino, Hidenori Daido, Yuka Ohmura, Namiko Takada, Yoshiki Itou, Akihiko Kondo, Hideki Fukuda, Nobuaki Shimizu

  Jun. 2007, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1774(6) (6), 671 - 678, English

  [Refereed]

  Scientific journal


• Construction of a novel synergistic system for production and recovery of secreted recombinant proteins by the cell surface engineering

  Seiji Shibasaki, Ai Kawabata, Jun Ishii, Shunsuke Yagi, Tetsuya Kadonosono, Michiko Kato, Nobuo Fukuda, Akihiko Kondo, Mitsuyoshi Ueda

  Jun. 2007, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 75(4) (4), 821 - 828, English

  [Refereed]

  Scientific journal


• Development of recombinant Aspergillus oryzae whole-cell biocatalyst expressing lipase-encoding gene from Candida antarctica

  Sriappareddy Tamalampudi, M. D. Mahabubur Rahman Talukder, Shinji Hama, Takanori Tanino, Yuya Suzuki, Akihiko Kondo, Hideki Fukuda

  May 2007, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 75(2) (2), 387 - 395, English

  [Refereed]

  Scientific journal


• Characterization of bio-nanocapsule as a transfer vector targeting human hepatocyte carcinoma by disulfide linkage modification

  Tadahiro Nagaoka, Takayuki Fukuda, Shinnosuke Yoshida, Hirohito Nishimura, Dongwei Yu, Shun'ichi Kuroda, Katsuyuki Tanizawa, Akihiko Kondo, Masakazu Ueda, Hidenori Yamada, Hiroko Tada, Masaharu Seno

  Apr. 2007, Journal of Controlled Release, 118(3) (3), 348 - 356, English

  [Refereed]

  Scientific journal


• Gene therapy of liver tumors with human liver-specific nanoparticles (vol 14, pg 74, 2007)

  M. Ueda, Y. Iwasaki, T. Yamada, A. Kondo, M. Seno, K. Tanizawa, S. Kuroda, M. Sakamoto, M. Kitajima

  Apr. 2007, CANCER GENE THERAPY, 14(4) (4), 440 - 440, English


• Production of L-lysine from starch by Corynebacterium glutamicum displaying alpha-amylase on its cell surface

  Toshihiro Tateno, Hideki Fukuda, Akihiko Kondo

  Apr. 2007, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 74(6) (6), 1213 - 1220, English

  [Refereed]

  Scientific journal


• Effect of cultivation conditions on cell-surface display of Flo1 fusion protein using sake yeast

  Takanori Tanino, Erina Noguchi, Sakurako Kimura, Hiroshi Sahara, Youji Hata, Hideki Fukuda, Akihiko Kondo

  Mar. 2007, BIOCHEMICAL ENGINEERING JOURNAL, 33(3) (3), 232 - 237, English

  [Refereed]

  Scientific journal


• Erythropoietin prevents the epileptogenesis and generalization in the epilepsy model rats

  Akihiko Kondo

  2007, Neuroscience Research

  Scientific journal


• 酵母を用いたリガンド検出システムの開発

  森口 みゆき, 石井 純, 立松 健司, 黒田 俊一, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2007, 化学工学会 研究発表講演要旨集, 2007, 844 - 844, Japanese


• アミラーゼ生産コリネ菌を用いたデンプンからのカダベリン生産

  岡田 勇介, 舘野 俊博, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2007, 化学工学会 研究発表講演要旨集, 2007, 818 - 818, Japanese


• AcmAタンパク質を用いた乳酸菌新規細胞表層提示システムの開発

  張 イ肖, 岡野 憲司, 木村 桜子, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2007, 化学工学会 研究発表講演要旨集, 2007, 817 - 817, Japanese


• 酵母表層ディスプレイを用いた迅速かつ高効率なタンパク質アフィニティ選択法の確立

  福田 展雄, 石井 純, 芝崎 誠司, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2007, 化学工学会 研究発表講演要旨集, 2007, 325 - 325, Japanese


• Candida antarctica 由来リパーゼB表層提示酵母を用いたエステル合成反応の検討

  谷野 孝徳, 大野 卓見, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2007, 化学工学会 研究発表講演要旨集, 2007, 413 - 413, Japanese


• An improved method of lipase preparation incorporating both solvent treatment and immobilization onto matrix

  Md. Mahabubur Rahman Talukder, Sriappareddy Tamalampudy, Chong Jia Li, Le Yanglin, Jinchuan Wu, Akihiko Kondo, Hideki Fukuda

  Jan. 2007, BIOCHEMICAL ENGINEERING JOURNAL, 33(1) (1), 60 - 65, English

  [Refereed]

  Scientific journal


• Fluorescence detection system for human G protein - Coupled receptor signaring in yeast

  Jun Ishii, Akihiko Kondo

  2007, PROGRESS ON POST-GENOME TECHNOLOGIES, 57 - 57, English

  [Refereed]

  International conference proceedings


• Enhancement of ethanol production by promoting surface contact between starch granules and arming yeast in direct ethanol fermentation

  Teik Seong Khaw, Yoshio Katakura, Kazuaki Ninomiya, Churairat Moukamnerd, Akihiko Kondo, Mitsuyoshi Ueda, Suteaki Shioya

  Jan. 2007, Journal of Bioscience and Bioengineering, 103(1) (1), 95 - 97, English

  [Refereed]

  Scientific journal


• Improvement of protein production in lactic acid bacteria using 5 '-untranslated leader sequence of slpA from Lactobacillus acidophilus

  Junya Narita, Saori Ishida, Kenji Okano, Sakurako Kimura, Hideki Fukuda, Akihiko Kondo

  Nov. 2006, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 73(2) (2), 366 - 373, English

  [Refereed]

  Scientific journal


• Effect of flocculation on performance of arming yeast in direct ethanol fermentation

  Khaw Teik Seong, Yoshio Katakura, Kazuaki Ninomiya, Yohei Bito, Satoshi Katahira, Akihiko Kondo, Mitsuyoshi Ueda, Suteaki Shioya

  Nov. 2006, Applied Microbiology and Biotechnology, 73(1) (1), 60 - 66, English

  [Refereed]

  Scientific journal


• Ethanol fermentation from lignocellulosic hydrolysate by a recombinant xylose- and cellooligosaccharide-assimilating yeast strain

  Satoshi Katahira, Atsuko Mizuike, Hideki Fukuda, Akihiko Kondo

  Oct. 2006, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 72(6) (6), 1136 - 1143, English

  [Refereed]

  Scientific journal


• Construction of a Pichia pastoris cell-surface display system using Flo1p anchor system

  Takanori Tanino, Hideki Fukuda, Akihiko Kondo

  Aug. 2006, BIOTECHNOLOGY PROGRESS, 22(4) (4), 989 - 993, English

  [Refereed]

  Scientific journal


• Development of novel yeast cell surface display system for homo-oligomeric protein by coexpression of native and anchored subunits

  Hirotaka Furukawa, Takanori Tanino, Hideki Fukuda, Akihiko Kondo

  Aug. 2006, BIOTECHNOLOGY PROGRESS, 22(4) (4), 994 - 997, English

  [Refereed]

  Scientific journal


• Enhancement of activity of lipase-displaying yeast cells and their application to optical resolution of (R,S)-1-benzyloxy-3-chloro-2-propyl monosuccinate

  Yurie Nakamura, Takeshi Matsumoto, Fumiki Nomoto, Mitsuyoshi Ueda, Hideld Fukuda, Akihiko Kondo

  Jul. 2006, Biotechnology Progress, 22(4) (4), 998 - 1002, English

  [Refereed]

  International conference proceedings


• Quantitative and dynamic analyses of G protein-coupled receptor signaling in yeast using Fus1, enhanced green fluorescence protein (EGFP), and His3 fusion protein

  Jun Ishii, Shizuka Matsumura, Sakurako Kimura, Kenji Tatematsu, Shun'ichi Kuroda, Hideki Fukuda, Akihiko Kondo

  Jul. 2006, Biotechnology Progress, 22(4) (4), 954 - 960, English

  [Refereed]

  International conference proceedings


• Display of active enzymes on the cell surface of Escherichia coli using PgsA anchor protein and their application to bioconversion

  J Narita, K Okano, T Tateno, T Tanino, T Sewaki, MH Sung, H Fukuda, A Kondo

  May 2006, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 70(5) (5), 564 - 572, English

  [Refereed]

  Scientific journal


• Evaluation of performance of different surface-engineered yeast strains for direct ethanol production from raw starch

  Teik Seong Khaw, Yoshio Katakura, Jun Koh, Akihiko Kondo, Mitsuyoshi Ueda, Suteaki Shioya

  May 2006, Applied Microbiology and Biotechnology, 70(5) (5), 573 - 579, English

  [Refereed]

  Scientific journal


• PCR-mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiae

  Rinji Akada, Takao Kitagawa, Shohei Kaneko, Daiso Toyonaga, Sachiko Ito, Yoshito Kakihara, Hisashi Hoshida, Shigeru Morimura, Akihiko Kondo, Kenji Kida

  Apr. 2006, Yeast, 23(5) (5), 399 - 405, English

  [Refereed]

  Scientific journal


• Lipase localization in Rhizopus oryzae cells immobilized within biomass support particles for use as whole-cell biocatalysts in biodiesel-fuel production

  S Hama, S Tamalampudi, T Fukumizu, K Miura, H Yamaji, A Kondo, H Fukuda

  Apr. 2006, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 101(4) (4), 328 - 333, English

  [Refereed]

  Scientific journal


• High-efficiency bioaffinity separation of cells and proteins using novel thermoresponsive biotinylated magnetic nanoparticles

  Noriyuki Ohnishi, Hirotaka Furukawa, Hata Hideyuki, Jing-Ming Wang, Chung-Il An, Eiichiro Fukusaki, Kazunori Kataoka, Katsuhiko Ueno, Akihiko Kondo

  Mar. 2006, Nanobiotechnology, 2(1-2) (1-2), 43 - 49, English

  [Refereed]

  Scientific journal


• Effect of cereal extracts and cereal fiber on viability of Lactobacillus plantarum under gastrointestinal tract conditions

  H Michida, S Tamalampudi, SS Pandiella, C Webb, H Fukuda, A Kondo

  Feb. 2006, BIOCHEMICAL ENGINEERING JOURNAL, 28(1) (1), 73 - 78, English

  [Refereed]

  Scientific journal


• BIOT 247-Preparation of lipases derivatives with high activity and stability by isopropanol treatment and immobilization on macroporous resin

  Akihiko Kondo

  2006, Abstracts of Papers of the American Chemical Society

  Scientific journal


• フェロモン応答性タンパク質を利用した新規レポーターによるリガンド検出システムの開発

  森口 みゆき, 石井 純, 立松 健司, 黒田 俊一, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2006, 化学工学会 研究発表講演要旨集, 2006, 986 - 986, Japanese


• Whole cell biocatalystによるバイオディーゼル燃料生産

  沼田 崇男, 福水 崇裕, 濱 真司, 山地 秀樹, 近藤 昭彦, 福田 秀樹

  公益社団法人 化学工学会, 2006, 化学工学会 研究発表講演要旨集, 2006, 950 - 950, Japanese


• 高機能Candida antarctica由来リパーゼB表層提示酵母の開発とエステル合成への応用

  谷野 孝徳, 大野 卓見, 鄭 源英, 渡辺 有香, 加藤 倫子, 植田 充美, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2006, 化学工学会 研究発表講演要旨集, 2006, 947 - 947, Japanese


• Development of dds using hollow bio-nanoparticles and their commercialization

  Akihiko Kondo, Shun-ichi Kuroda, Katsuyuki Tanizawa, Masaharu Seno, Masakazu Ueda

  2006, Drug Delivery System, 21(4) (4), 435 - 443, English

  [Refereed]

  Scientific journal


• Development of a single cell analysis system of agonist for drug discovery

  Akihiko Kondo, Jun Ishii, Shizuka Matsumura, Hideki Fukuda

  2006, Progress on Post-Genome Technologies, 30 - 30, English

  [Refereed]

  International conference proceedings


• Engineered bio-nanocapsules, the selective vector for drug delivery system

  DW Yu, T Fukuda, Tuoya, S Kuroda, K Tanizawa, A Kondo, M Ueda, T Yamada, H Tada, M Seno

  Jan. 2006, IUBMB LIFE, 58(1) (1), 1 - 6, English

  [Refereed]

  Scientific journal


• Display of alpha-amylase on the surface of Lactobacillus casei cells by use of the PgsA anchor protein, and production of lactic acid from starch

  J Narita, K Okano, T Kitao, S Ishida, T Sewaki, MH Sung, H Fukuda, A Kondo

  Jan. 2006, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72(1) (1), 269 - 275, English

  [Refereed]

  Scientific journal


• The specific delivery of proteins to human liver cells by engineered bio-nanocapsules

  DW Yu, C Amano, T Fukuda, T Yamada, S Kuroda, K Tanizawa, A Kondo, M Ueda, H Yamada, H Tada, M Seno

  Jul. 2005, FEBS JOURNAL, 272(14) (14), 3651 - 3660, English

  [Refereed]

  Scientific journal


• Deletion of MCD4 involved in glycosylphosphatidylinositol (GPI) anchor synthesis leads to an increase in beta-1,6-glucan level and a decrease in GPI-anchored protein and mannan levels in the cell wall of Saccharomyces cerevisiae

  J Maneesri, M Azuma, Y Sakai, K Igarashi, T Matsumoto, H Fukuda, A Kondo, H Ooshima

  Apr. 2005, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 99(4) (4), 354 - 360, English

  [Refereed]

  Scientific journal


• Deletion of MCD4 involved in glycosylphosphatidylinositol (GPI) anchor synthesis leads to an increase in beta-1,6-glucan level and a decrease in GPI-anchored protein and mannan levels in the cell wall of Saccharomyces cerevisiae

  J Maneesri, M Azuma, Y Sakai, K Igarashi, T Matsumoto, H Fukuda, A Kondo, H Ooshima

  Apr. 2005, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 99(4) (4), 354 - 360, English

  [Refereed]

  Scientific journal


• Facilitatory effect of immobilized lipase-producing Rhizopus oryzae cells on acyl migration in biodiesel-fuel production

  M Oda, M Kaieda, S Hama, H Yamaji, A Kondo, E Izumoto, H Fukuda

  Mar. 2005, BIOCHEMICAL ENGINEERING JOURNAL, 23(1) (1), 45 - 51, English

  [Refereed]

  Scientific journal


• 昆虫細胞を用いたバイオナノ粒子の効率的生産

  宍戸 卓矢, 村岡 優, 上田 政和, 妹尾 昌治, 多田 宏子, 谷澤 克行, 黒田 俊一, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2005, 化学工学会 研究発表講演要旨集, 2005, 327 - 327, Japanese


• PgsAタンパク質を用いた大腸菌および乳酸菌細胞表層提示システムの開発

  岡野 憲司, 成田 純也, 瀬脇 智満, 成 文喜, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2005, 化学工学会 研究発表講演要旨集, 2005, 255 - 255, Japanese


• FLO1由来アンカーを用いたPichia pastorisを宿主細胞とした酵母細胞表層提示技術の開発

  谷野 孝徳, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2005, 化学工学会 研究発表講演要旨集, 2005, 256 - 256, Japanese


• Whole-cell biocatalystにおける糸状菌リパーゼの局在解析

  ? 真司, 近藤 昭彦, 福田 秀樹

  公益社団法人 化学工学会, 2005, 化学工学会 研究発表講演要旨集, 2005, 288 - 288, Japanese


• 糖トランスポーターの発現による酵母のエタノール生産能力の向上

  伊藤 環, 片平 悟史, 近藤 昭彦, 福田 秀樹

  公益社団法人 化学工学会, 2005, 化学工学会 研究発表講演要旨集, 2005, 240 - 240, Japanese


• ピンポイントデリバリー用バイオナノキャリアの開発とがん遺伝子治療への応用

  近藤 昭彦

  2005, 厚生労働科学研究費補助金萌芽的先端医療技術推進研究事業平成16年度研究報告書, 83(2),91, Japanese

  Scientific journal


• Rot1p of Saccharomyces cerevisiae is a putative membrane protein required for normal levels of the cell wall 1,6-beta-glucan

  K Machi, M Azuma, K Igarashi, T Matsumoto, H Fukuda, A Kondo, H Oshima

  Oct. 2004, MICROBIOLOGY-SGM, 150, 3163 - 3173, English

  [Refereed]

  Scientific journal


• Effect of fatty acid membrane composition on whole-cell biocatalysts for biodiesel-fuel production

  S Hama, H Yamaji, M Kaieda, M Oda, A Kondo, H Fukuda

  Oct. 2004, BIOCHEMICAL ENGINEERING JOURNAL, 21(2) (2), 155 - 160, English

  [Refereed]

  Scientific journal


• Construction of a xylan-fermenting yeast strain through codisplay of xylanolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells

  S Katahira, Y Fujita, A Mizuike, H Fukuda, A Kondo

  Sep. 2004, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(9) (9), 5407 - 5414, English

  [Refereed]

  Scientific journal


• Enantioselective transesterification using immobilized Aspergillus oryzae overexpressing lipase

  M Kaieda, M Nagayoshi, S Hama, A Kondo, H Fukuda

  Aug. 2004, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 65(3) (3), 301 - 305, English

  [Refereed]

  Scientific journal


• Direct production of ethanol from raw corn starch via fermentation by use of a novel surface-engineered yeast strain codisplaying glucoamylase and alpha-amylase

  H Shigechi, J Koh, Y Fujita, T Matsumoto, Y Bito, M Ueda, E Satoh, H Fukuda, A Kondo

  Aug. 2004, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(8) (8), 5037 - 5040, English

  [Refereed]

  Scientific journal


• Combinatorial bioengineering - Development of molecular evolution

  M Ueda, A Kondo

  Jun. 2004, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 28(4-6) (4-6), 137 - 137, English

  [Refereed]


• Efficient production of L-(+)-lactic acid from raw starch by Streptococcus bovis 148

  J Narita, S Nakahara, H Fukuda, A Kondo

  Jun. 2004, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 97(6) (6), 423 - 425, English

  [Refereed]

  Scientific journal


• Over-expression system for secretory phospholipase D by Streptomyces lividans

  C Ogino, M Kanemasu, Y Hayashi, A Kondo, N Shimizu, S Tokuyama, Y Tahara, S Kuroda, K Tanizawa, H Fukuda

  Jun. 2004, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 64(6) (6), 823 - 828, English

  [Refereed]

  Scientific journal


• Molecular engineering of Rhizopus oryzae lipase using a combinatorial protein library constructed on the yeast cell surface

  H Shibamoto, T Matsumoto, H Fukuda, A Kondo

  Jun. 2004, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 28(4-6) (4-6), 235 - 239, English

  [Refereed]

  Scientific journal


• Isolation of novel catalytic antibody clones from combinatorial library displayed on yeast-cell surface

  Y Lin, S Shiraga, T Tsumuraya, Fujii, I, T Matsumoto, A Kondo, M Ueda

  Jun. 2004, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 28(4-6) (4-6), 247 - 251, English

  [Refereed]

  Scientific journal


• Construction of system for localization of target protein in yeast periplasm using invertase

  T Tanino, T Matsumoto, H Fukuda, A Kondo

  Jun. 2004, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 28(4-6) (4-6), 259 - 264, English

  [Refereed]

  Scientific journal


• Comparison of two forms of catalytic antibody displayed on yeast-cell surface

  Y Lin, S Shiraga, T Tsumuraya, T Matsumoto, A Kondo, Fujii, I, M Ueda

  Jun. 2004, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 28(4-6) (4-6), 241 - 246, English

  [Refereed]

  Scientific journal


• Over-expression system of phospholipase D from actinomycete by Streptomyces lividans

  OGINO Chiaki, KANEMASU M, HAYASHI Y, KURODA S, KONDO Akihiko, SHMIZU N, TANIZAWA K, FUKUDA Hideki

  Jun. 2004, Applied Microbiology and Biotechnology, Vol 64, pp. 823-828, English

  [Refereed]

  Scientific journal


• Improvement of cellulose-degrading ability of a yeast strain displaying Trichoderma reesei endoglucanase II by recombination of cellulose-binding domains

  J Ito, Y Fujita, M Ueda, H Fukuda, A Kondo

  May 2004, BIOTECHNOLOGY PROGRESS, 20(3) (3), 688 - 691, English

  [Refereed]

  Scientific journal


• Improvement of cellulose-degrading ability of a yeast strain displaying Trichoderma reesei endoglucanase II by recombination of cellulose-binding domains

  J Ito, Y Fujita, M Ueda, H Fukuda, A Kondo

  May 2004, BIOTECHNOLOGY PROGRESS, 20(3) (3), 688 - 691, English

  [Refereed]

  Scientific journal


• Energy-saving direct ethanol production from low-temperature-cooked corn starch using a cell-surface engineered yeast strain co-displaying glucoamylase and alpha-amylase

  H Shigechi, Y Fujita, J Koh, M Ueda, H Fukuda, A Kondo

  May 2004, BIOCHEMICAL ENGINEERING JOURNAL, 18(2) (2), 149 - 153, English

  [Refereed]

  Scientific journal


• Energy-saving direct ethanol production from low-temperature-cooked corn starch using a cell-surface engineered yeast strain co-displaying glucoamylase and alpha-amylase

  H Shigechi, Y Fujita, J Koh, M Ueda, H Fukuda, A Kondo

  May 2004, BIOCHEMICAL ENGINEERING JOURNAL, 18(2) (2), 149 - 153, English

  [Refereed]

  Scientific journal


• Refolding of proteins by hexadecamers and monomers of the alpha and beta subunits of group II chaperonin from the hyperthermophilic archaeum Thermococcus strain KS-1

  J Kohda, T Yamada, T Yoshida, T Maruyama, M Yohda, H Fukuda, A Kondo

  Apr. 2004, BIOCHEMICAL ENGINEERING JOURNAL, 18(1) (1), 73 - 79, English

  [Refereed]

  Scientific journal


• Yeast cell-surface display - applications of molecular display

  A Kondo, M Ueda

  Mar. 2004, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 64(1) (1), 28 - 40, English

  [Refereed]

  Scientific journal


• Synergistic saccharification, and direct fermentation to ethanol, of amorphous cellulose by use of an engineered yeast strain codisplaying three types of cellulolytic enzyme

  Y Fujita, J Ito, M Ueda, H Fukuda, A Kondo

  Feb. 2004, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(2) (2), 1207 - 1212, English

  [Refereed]

  Scientific journal


• Structure and properties of cathodic arc ion plated CrN coatings for copper machining cutting tools

  A. Kondo, T. Oogami, K. Sato, Y. Tanaka

  Elsevier, Jan. 2004, Surface and Coatings Technology, 177-178, 238 - 244, English

  [Refereed]

  Scientific journal


• A Kinetic Study of Direct Ethanol Production from Raw Starch by Various Arming Yeast Strains

  Khaw Teik-Seong, Katakura Yoshio, Koh Jun, Kondo Akihiko, Shioya Suteaki

  Single step bioconversion of raw starch to ethanol has raised considerable interest in recent years. We have developed yeast strains displaying α-amylase and glucoamylase on yeast cell surface for this purpose. The advantage of this system is that the displayed enzymes can be recovered easily with the yeast after fermentation compared to the case that these amylolytic enzymes are secreted into medium. However, display of α-amylase on cell surface may reduce the apparent association rate of the enzyme to the substrate because both of them are huge molecules, resulting a lower apparent conversion rate. The recovery of the yeast cells together with the amylolytic enzymes becomes easier when a flocculating yeast strain is used as the host strain. However, flocculation of the arming yeast may again reduce the apparent conversion rate. To establish an industrial ethanol production process with a good cost performance ratio, the advantages and disadvantages of each strategy should be evaluated quantitatively. Therefore, from a viewpoint of kinetics, we compared the performance of the fermentation systems in the case that α-amylase is displayed on the cell surface or secreted in the medium, and in the cases that a flocculating or non-flocculating strain is used as the host strain. A kinetic model has been developed to simulate the direct ethanol fermentation from raw corn starch by arming yeast. The simulation results were found agree well with experimental data obtained from direct ethanol fermentation.

  The Society of Chemical Engineers, Japan, 2004, Asian Pacific Confederation of Chemical Engineering congress program and abstracts, 2004, 789 - 789, English


• Biodiesel Fuel Production using Fungus- and Yeast-Whole Cell Biocatalysts

  Fukuda Hideki, Kondo Akihiko, Noda Hideo

  With a view to utilizing Rhizopus oryzae cells immobilized within biomass support particles (BSPs) as a whole-cell biocatalyst for biodiesel fuel production, an investigation was made of the effect of cross-linking treatment with glutaraldehyde on the stability of lipase activity. Although the lipase activity of the BSP-immobilized cells decreased considerably in the presence of the methyl esters produced by methanolysis, the activity of cells treated with 0.1% glutaraldehyde solution showed no significant decrease during twenty batch cycles, with the methyl ester content of the reaction mixture reaching 60-83% in each cycle. We also constructed a novel cell-surface display system using as a new type of cell-wall anchor the 3297-bp of the 3' region of the FLO1 gene, and in this system the N-terminus of the Rhizopus oryzae lipase (ROL) was fused to the FLO1 protein. In the use of Saccharomyces cerevisiae cells displaying FLO1 gene as a whole-cell biocatalyst methyl ester content synthesized reached 78.3% after 72 h. These findings indicate that, given the simplicity of the lipase production process, the use of whole cell biocatalysts using either R. oryzae cells immobilized within BSPs or S. cerevisiae cells displaying ROL offers a promising means of biodiesel fuel production for industrial application.

  The Society of Chemical Engineers, Japan, 2004, Asian Pacific Confederation of Chemical Engineering congress program and abstracts, 2004, 455 - 455, English


• Efficient Production of Ethanol from Biomass by Cell Surface Engineered Yeast Strains

  Kondo Akihiko, Fujita Yasuya, Fukuda Hideki

  Display of novel enzymes on the yeast cell surface is very powerful method to develop the efficient whole cell biocatalysts, because the diffusion problem of substrate and product is circumvented. In addition, the displayed enzymes are regarded as a kind of self-immobilized enzyme on the cell surfaces. In this study, we have developed the novel methods to display enzymes using cell wall anchoring proteins -agglutinin. To show their applicability to the productions of biofuels and chemicals from biomass, the ethanol production from cellulosic materials was investigated by using yeast cells displaying cellulolytic enzymes. Due to the display of these enzymes, cellulosic materials were sequentially hydrolyzed to glucose on the yeast cell surface, immediately utilized and converted to ethanol by intracellular enzymes. The yield in terms of grams of ethanol produced per grams of carbohydrate utilized was over 0.45, which corresponds to over 89% of theoretical yield. These results demonstrate that a combination of cell surface displayed enzymes and intracellular metabolic system is a very effective approach to develop cells with novel catalytic and metabolic abilities for industrial applications.

  The Society of Chemical Engineers, Japan, 2004, Asian Pacific Confederation of Chemical Engineering congress program and abstracts, 2004, 427 - 427, English


• Pinpoint drug delivery system using hollow bio-nanoparticles

  T Yamada, M Seno, A Kondo, M Ueda, K Tanizawa, S Kuroda

  2004, KOBUNSHI RONBUNSHU, 61(12) (12), 606 - 612, Japanese

  [Refereed]

  Scientific journal


• 熱応答性磁性ナノ粒子の開発とポストゲノムシーケンスへの応用

  大西 徳幸, 近藤 昭彦

  シーエムシー出版, 2004, バイオインダストリー, 21(8),31-38(8) (8), 31 - 38, Japanese

  Scientific journal


• 熱応答性磁性ナノ粒子(Therma-Max)の開発とそのバイオ領域への応用

  大西 徳幸, 近藤 昭彦

  日刊工業新聞社, 2004, 工業材料, 52,57-61(10) (10), 57 - 61, Japanese

  Scientific journal


• 熱応答性磁性ナノ粒子(Therma-Max)の開発とそのバイオ領域への応用

  大西 徳幸, 近藤 昭彦

  日刊工業新聞社, 2004, MATERIAL ATAGE, 4(5),54-57(10) (10), 57 - 61, Japanese

  Scientific journal


• 熱応答性高分子を利用した磁性ナノ粒子の新しい分離法

  近藤 昭彦, 大西 徳幸

  日本農芸化学会, 2004, 化学と生物, 485,212-216(4) (4), 213 - 216, Japanese

  Scientific journal


• 中空バイオナノ粒子を用いたピポイントドラッグデリバリーシステム

  YAMADA Tadanori, SENOO Masaharu, KONDO Akihiko, UEDA Masakazu, TANIZAWA Katsuyuki, KURODA Syunnichi

  2004, 高分子論文集, 61,606-612, Japanese

  [Refereed]

  Scientific journal


• 中空バイオナノ粒子によるピンポイントDDS

  近藤 昭彦

  2004, フォルマシア, 40,1013-1017, Japanese

  Scientific journal


• 中空バイオナノ粒子が拓く新しい医療技術

  黒田 俊一, 山田 忠徳, 妹尾 昌治, 近藤 昭彦, 上田 政和, 谷澤 克行

  化学工業社, 2004, 化学工業, 40,936-942(12) (12), 936 - 942, Japanese

  Scientific journal


• 多彩な細胞表層ディスプレイシステム

  近藤 昭彦

  日本生物工学会, 2004, 生物工学会誌, 82,454(9) (9), 454 - 454, Japanese

  Scientific journal


• アーミング技術を用いた微生物によるバイオマス変換の高効率化

  近藤 昭彦

  2004, グリーンプラジャーナル, 12,10-13, Japanese

  Scientific journal


• Synergistic Saccahrificatin and Direct Fermentation to ethanol of Amorphous Cellulose by Use of an Engineered Yeast Strain Codisplayinjg Three Types of Cellulolytic Enzyme

  FUJITA Yasuya, ITO Junji, UEDA Mitsuyoshi, FUKUDA Hideki, KONDO Akihiko

  American Society for Microbiology, 2004, Applied and Environmental Microbiology, 70(2),1207-1212(2) (2), 1207 - 1212, English

  [Refereed]

  Scientific journal


• Over-expression system for secretory phospholipase D by Streptomyces livedanse

  OGINO Chiaki, KANEMASU Masayuki, HAYASI Yasuhiro, KONDO AKIHIKO, SHIMIZU Noriaki, TOKUYAMA Shinji, TAHARA Y, KURODA Syunicni, TANIZAWA Katsyuyuki, FUKUDA Hideki

  2004, Applied and Microbiogy and Biotechnology, 64,823-828, English

  [Refereed]

  Scientific journal


• Novel tissue and cell type-specific gene/drug delivery system using surface engineered hepatitis B virus nano-particles

  Tadanori Yamada, Masakazu Ueda, Masaharu Seno, Akihiko Kondo, Katsuyuki Tanizawa, Shun'ichi Kuroda

  2004, Current Drug Targets - Infectious Disorders, 4(2) (2), 163 - 167, English

  [Refereed]

  Scientific journal


• Enantioselective Transesterification Using Lipase-Displaying Yeast Whole-Cell Biocatalyst

  MATSUMOTO Takeshi, ITO Meguru, FUKUDA Hideki, KONDO Akihiko

  日本生物工学会, 2004, Applied Microbiology and Biotechnology, 64 481-485, 88 - 88, English

  [Refereed]

  Scientific journal


• Enantioselective Transesterification Using Lipase-Displaying Yeast Whole-Cell Biocatalyst

  MATSUMOTO Takeshi, ITO M, FUKUDA Hideki, KONDO Akihiko

  日本生物工学会, 2004, Applied Microbiology and Biatechnology, 64,481-485, 88 - 88, English

  [Refereed]

  Scientific journal


• Construction of ethanol-tolerant yeast strains with combinatorial library-selected peptides

  Akihiko Kondo

  2004, Journal of Molecular Catalysis B: Enzymatic, 28(4-6) 253-257, English

  [Refereed]

  Scientific journal


• Construction of Ethanol-Telerant yeast Strains with Combinatorial Library-Selected Peptides

  MATSUMOTO Takeshi, ISHIKAWA Shinya, FUKUDA Hideki, KONDO Akihiko

  2004, Journal of Molecular Catalysis B, 28(4-6),253-257, English

  [Refereed]

  Scientific journal


• Preparation of yeast strains displaying IgG binding domain ZZ and enhanced green fluorescent protein for novel antigen detection systems

  R Shimojyo, H Furukawa, H Fukuda, A Kondo

  Nov. 2003, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 96(5) (5), 493 - 495, English

  [Refereed]

  Scientific journal


• Affinity selection of target cells from cell surface displayed libraries: a novel procedure using thermo-responsive magnetic nanoparticles

  H Furukawa, R Shimojyo, N Ohnishi, H Fukuda, A Kondo

  Oct. 2003, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 62(5-6) (5-6), 478 - 483, English

  [Refereed]

  Scientific journal


• Phospholipase D from Streptoverticillium cinnamoneum: protein engineering and application for phospholipid production

  C Ogino, S Kuroda, S Tokuyama, A Kondo, N Shimizu, K Tanizawa, H Fukuda

  Sep. 2003, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 23(2-6) (2-6), 107 - 115, English

  [Refereed]

  Scientific journal


• Display of a functional hetero-oligomeric catalytic antibody on the yeast cell surface

  Y Lin, T Tsumuraya, T Wakabayashi, S Shiraga, Fujii, I, A Kondo, M Ueda

  Aug. 2003, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 62(2-3) (2-3), 226 - 232, English

  [Refereed]

  Scientific journal


• Microstructure and mechanical properties of cathodic arc ion-plated (Al,Ti)N coatings

  K. Sato, N. Ichimiya, A. Kondo, Y. Tanaka

  Jan. 2003, Surface and Coatings Technology, 163-164, 135 - 143, English

  [Refereed]

  Scientific journal


• Xylan加水分解酵素を細胞表層に提示したxylose資化酵母によるxylanからのethanol生産

  片平 悟史, 藤田 靖也, 植田 充美, 田中 渥夫, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2003, 化学工学会 研究発表講演要旨集, 2003, 139 - 139, Japanese


• Whole cell biocatalystによるメタノリシス反応の解析

  小田 充宏, 海江田 優, 浜 真司, 近藤 昭彦, 福田 秀樹

  公益社団法人 化学工学会, 2003, 化学工学会 研究発表講演要旨集, 2003, 130 - 130, Japanese


• Long anchor using Flo1 protein enhances reactivity of cell surface-displayed glucoamylase to polymer substrates

  N. Sato, T. Matsumoto, M. Ueda, A. Tanaka, H. Fukuda, A. Kondo

  2003, Applied Microbiology and Biotechnology, 60(4) (4), 469 - 474, English

  [Refereed]

  Scientific journal


• Efficient secretory overexpression of Bacillus subtilis pectate lyase in Escherichia coli and single-step purification

  T Matsumoto, D Katsura, A Kondo, H Fukuda

  Dec. 2002, BIOCHEMICAL ENGINEERING JOURNAL, 12(3) (3), 175 - 179, English

  [Refereed]

  Scientific journal


• Direct and efficient production of ethanol from cellulosic material with a yeast strain displaying cellulolytic enzymes

  Yasuya Fujita, Shouji Takahashi, Mitsuyoshi Ueda, Atsuo Tanaka, Hirofumi Okada, Yasushi Morikawa, Takashi Kawaguchi, Motoo Arai, Hideki Fukuda, Akihiko Kondo

  Oct. 2002, Applied and Environmental Microbiology, 68(10) (10), 5136 - 5141, English

  [Refereed]

  Scientific journal


• Construction of yeast strains with high cell surface lipase activity by using novel display systems based on the Flo1p flocculation functional domain

  Takeshi Matsumoto, Hideki Fukuda, Mitsuyoshi Ueda, Atsuo Tanaka, Akihiko Kondo

  Sep. 2002, Applied and Environmental Microbiology, 68(9) (9), 4517 - 4522, English

  [Refereed]

  Scientific journal


• Effect of oxidized and reduced forms of Escherichia coli DsbC on protein refolding

  J Kohda, N Kawahara, H Fukuda, A Kondo

  Aug. 2002, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 94(2) (2), 130 - 134, English

  [Refereed]

  Scientific journal


• Efficient ethanol production from starch through development of novel flocculent yeast strains displaying glucoamylase and co-displaying or secreting α-amylase

  Hisayori Shigechi, Ken Uyama, Yasuya Fujita, Takeshi Matsumoto, Mitsuyoshi Ueda, Atsuo Tanaka, Hideki Fukuda, Akihiko Kondo

  Jun. 2002, Journal of Molecular Catalysis - B Enzymatic, 17(3-5) (3-5), 179 - 187, English

  [Refereed]

  Scientific journal


• Construction of whole-cell biocatalyst for xylan degradation through cell-surface xylanase display in Saccharomyces cerevisiae

  Yasuya Fujita, Satoshi Katahira, Mitsuyoshi Ueda, Atsuo Tanaka, Hirofumi Okada, Yasushi Morikawa, Hideki Fukuda, Akihiko Kondo

  Jun. 2002, Journal of Molecular Catalysis - B Enzymatic, 17(3-5) (3-5), 189 - 195, English

  [Refereed]

  Scientific journal


• Construction of engineered yeast with the ability of binding to cellulose

  Jin-Min Nam, Yasuya Fujita, Terukazu Arai, Akihiko Kondo, Yasushi Morikawa, Hirofumi Okada, Mitsuyoshi Ueda, Atsuo Tanaka

  Jun. 2002, Journal of Molecular Catalysis - B Enzymatic, 17(3-5) (3-5), 197 - 202, English

  [Refereed]

  Scientific journal


• Preparation of high activity yeast whole cell bioctalysts by optimization of intracellular production of recombinant Rhizopus oryzae lipase

  Takeshi Matsumoto, Shouji Takahashi, Mitsuyoshi Ueda, Atsuo Tanaka, Hideki Fukuda, Akihiko Kondo

  Jun. 2002, Journal of Molecular Catalysis - B Enzymatic, 17(3-5) (3-5), 143 - 149, English

  [Refereed]

  Scientific journal


• Repeated use of whole-cell biocatalysts immobilized within biomass support particles for biodiesel fuel production

  K Ban, S Hama, K Nishizuka, M Kaieda, T Matsumoto, A Kondo, H Noda, H Fukuda

  Jun. 2002, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 17(3-5) (3-5), 157 - 165, English

  [Refereed]

  Scientific journal


• Improvement of transphosphatidylation reaction model of phospholipase D from Streptoverticillium cinnamoneum

  C Ogino, Y Yasuda, A Kondo, N Shimizu, H Fukuda

  Mar. 2002, BIOCHEMICAL ENGINEERING JOURNAL, 10(2) (2), 115 - 121, English

  [Refereed]

  Scientific journal


• Improvement of productivity of active form of glutamate racemase in Escherichia coli by coexpression of folding accessory proteins

  J Kohda, Y Endo, N Okumura, Y Kurokawa, K Nishihara, H Yanagi, T Yura, H Fukuda, A Kondo

  Feb. 2002, BIOCHEMICAL ENGINEERING JOURNAL, 10(1) (1), 39 - 45, English

  [Refereed]

  Scientific journal


• Development of new surface display systems for combinatorial bioengineering

  A Kondo, T Matsumoto, H Fukuda

  2002, 6TH WORLD MULTICONFERENCE ON SYSTEMICS, CYBERNETICS AND INFORMATICS, VOL VI, PROCEEDINGS, 431 - 435, English

  [Refereed]

  International conference proceedings


• High-level ethanol production from starch by a flocculent Saccharomyces cerevisiaestrain displaying cell-surface glucoamylase

  A. Kondo, H. Shigechi, M. Abe, K. Uyama, T. Matsumoto, S. Takahashi, M. Ueda, A. Tanaka, M. Kishimoto, H. Fukuda

  2002, Applied Microbiology and Biotechnology, 58(3) (3), 291 - 296, English

  [Refereed]

  Scientific journal


• Development of novel whole-cell immunoadsorbents by yeast surface display of the IgG-binding domain

  Y Nakamura, S Shibasaki, M Ueda, A Tanaka, H Fukuda, A Kondo

  Nov. 2001, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 57(4) (4), 500 - 505, English

  [Refereed]

  Scientific journal


• Whole cell biocatalyst for biodiesel fuel production utilizing Rhizopus oryzae cells immobilized within biomass support particles

  K Ban, M Kaieda, T Matsumoto, A Kondo, H Fukuda

  Jul. 2001, BIOCHEMICAL ENGINEERING JOURNAL, 8(1) (1), 39 - 43, English

  [Refereed]

  Scientific journal


• コンビナトリアル・バイオエンジニアリング 細胞表層ディスプレイ法による新機能細胞の創製

  藤田靖也, 松本健史, 近藤昭彦, 植田充美

  シ-エムシ-, Jun. 2001, Bio Ind, 18(6) (6), 56 - 63, Japanese

  [Refereed]


• Effect of methanol and water contents on production of biodiesel fuel from plant oil catalyzed by various lipases in a solvent-free system

  M Kaieda, T Samukawa, A Kondo, H Fukuda

  Jan. 2001, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 91(1) (1), 12 - 15, English

  [Refereed]

  Scientific journal


• Effective biofuel production by an intelligent bioreactor

  H Fukuda, A Kondo, H Noda

  2001, ENGINEERING AND MANUFACTURING FOR BIOTECHNOLOGY, VOL 4, 4, 449 - 455, English

  [Refereed]

  International conference proceedings


• Technology 細胞表層ディスプレイ系の開発と応用

  近藤 昭彦, 植田 充美

  メディカルドゥ, 2001, 遺伝子医学, 5(2) (2), 299 - 304, Japanese

  [Refereed]


• Yeast whole-cell biocatalyst constructed by intracellular overproduction of Rhizopus oryzae lipase is applicable to biodiesel fuel production

  T. Matsumoto, S. Takahashi, M. Kaieda, M. Ueda, A. Tanaka, H. Fukuda, A. Kondo

  2001, Applied Microbiology and Biotechnology, 57(4) (4), 515 - 520, English

  [Refereed]

  Scientific journal


• Identification of novel membrane-bound phospholipase D from Streptoverticillium cinnamoneum, possessing only hydrolytic activity

  C Ogino, Y Negi, H Daido, M Kanemasu, A Kondo, S Kuroda, K Tanizawa, N Shimizu, H Fukuda

  Jan. 2001, BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1530(1) (1), 23 - 31, English

  [Refereed]

  Scientific journal


• Improvement of productivity of active horseradish peroxidase in Escherichia coli by coexpression of Dsb proteins

  A Kondo, J Kohda, Y Endo, T Shiromizu, Y Kurokawa, K Nishihara, H Yanagi, T Yura, H Fukuda

  Dec. 2000, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 90(6) (6), 600 - 606, English

  [Refereed]

  Scientific journal


• Preparation of high activity whole cell biocatalyst by permeabilization of recombinant flocculent yeast with alcohol

  A Kondo, Y Liu, M Furuta, Y Fujita, T Matsumoto, H Fukuda

  Dec. 2000, ENZYME AND MICROBIAL TECHNOLOGY, 27(10) (10), 806 - 811, English

  [Refereed]

  Scientific journal


• Efficient production of protopectinases by Bacillus subtilis using medium based on soybean flour

  T Matsumoto, Y Sugiura, A Kondo, H Fukuda

  Oct. 2000, BIOCHEMICAL ENGINEERING JOURNAL, 6(2) (2), 81 - 86, English

  [Refereed]

  Scientific journal


• Pretreatment of immobilized Candida antarctica lipase for biodiesel fuel production from plant oil

  T Samukawa, M Kaieda, T Matsumoto, K Ban, A Kondo, Y Shimada, H Noda, H Fukuda

  Aug. 2000, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 90(2) (2), 180 - 183, English

  [Refereed]

  Scientific journal


• Preparation of high-activity whole cell biocatalysts by permeabilization of recombinant yeasts with alcohol

  Y Liu, Y Fujita, A Kondo, H Fukuda

  Jun. 2000, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 89(6) (6), 554 - 558, English

  [Refereed]

  Scientific journal


• Analysis of the interaction between monoclonal antibodies and human hemoglobin (native and cross-linked) using a surface plasmon resonance (SPR) biosensor

  Shuji Sonezaki, Shinichi Yagi, Emika Ogawa, Akihiko Kondo

  Apr. 2000, Journal of Immunological Methods, 238(1-2) (1-2), 99 - 106, English

  [Refereed]

  Scientific journal


• Development of efficient protein refolding systems using chaperonins

  J Kohda, A Kondo, T Teshima, H Fukuda

  2000, BIOSEPARATION ENGINEERING, 16, 119 - 124, English

  [Refereed]

  International conference proceedings


• Biodiesel fuel production from plant oil catalyzed by Rhizopus oryzae lipase in a water-containing system without an organic solvent

  M Kaieda, T Samukawa, T Matsumoto, K Ban, A Kondo, Y Shimada, H Noda, F Nomoto, K Ohtsuka, E Izumoto, H Fukuda

  Dec. 1999, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 88(6) (6), 627 - 631, English

  [Refereed]

  Scientific journal


• Preparation of thermo-sensitive magnetic microspheres and their application to bioprocesses

  A Kondo, H Fukuda

  Aug. 1999, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 153(1-3) (1-3), 435 - 438, English

  [Refereed]

  Scientific journal


• Production of S-lactoylglutathione by high activity whole cell biocatalysts prepared by permeabilization of recombinant Saccharomyces cerevisiae with alcohols

  Y Liu, H Hama, Y Fujita, A Kondo, Y Inoue, A Kimura, H Fukuda

  Jul. 1999, BIOTECHNOLOGY AND BIOENGINEERING, 64(1) (1), 54 - 60, English

  [Refereed]

  Scientific journal


• Purification, characterization, and sequence determination of phospholipase D secreted by Streptoverticillium cinnamoneum

  C Ogino, Y Negi, T Matsumiya, K Nakaoka, A Kondo, S Kuroda, S Tokuyama, U Kikkawa, T Yamane, H Fukuda

  Feb. 1999, JOURNAL OF BIOCHEMISTRY, 125(2) (2), 263 - 269, English

  [Refereed]

  Scientific journal


• Bioconversion using immobilized recombinant flocculent yeast cells carrying a fused enzyme gene in an 'intelligent' bioreactor

  Y Liu, A Kondo, H Ohkawa, N Shiota, H Fukuda

  Dec. 1998, BIOCHEMICAL ENGINEERING JOURNAL, 2(3) (3), 229 - 235, English

  [Refereed]

  Scientific journal


• Effects of adsorption conditions on kinetics of protein adsorption and conformational changes at ultrafine silica particles

  A Kondo, H Fukuda

  Feb. 1998, JOURNAL OF COLLOID AND INTERFACE SCIENCE, 198(1) (1), 34 - 41, English

  [Refereed]

  Scientific journal


• Synthesis of LiMnO₂ with α-NaMnO₂-type structure by a mixed-alkaline hydrothermal reaction

  Akihiko Kondo

  1998, Journal of the Electrochemical Society

  Scientific journal


• Production of MBP(maltose binding protein)-GroES fusion protein and utilization to stimulate GroEL-mediated protein refolding

  Yoshiyuki Ishii, Kaoru Murakami, Hiroaki I-Ogawa, Akihiko Kondo, Yasuhiko Kato

  Elsevier Sci B.V., 1998, Journal of Fermentation and Bioengineering, 85(1) (1), 69 - 73, English

  [Refereed]

  Scientific journal


• Affinity purification and immobilization of fusion chaperonin GroEL- (His)6 and its utilization to mediate protein refolding

  Tadanaru Teshima, Shinobu Mashimo, Akihiko Kondo, Hideki Fukuda

  Elsevier Sci B.V., 1998, Journal of Fermentation and Bioengineering, 86(4) (4), 357 - 362, English

  [Refereed]

  Scientific journal


• Single-step purification and characterization of MBP (maltose binding protein) - DnaJ fusion protein and its utilization for structure-function analysis

  Yoshiyuki Ishii, Syuji Sonezaki, Yasushi Iwasaki, Eiichi Tauchi, Yoshikazu Shingu, Koichi Okita, Hiroaki I.-Ogawa, Yasuhiko Kato, Akihiko Kondo

  Japanese Biochemical Society, 1998, Journal of Biochemistry, 124(4) (4), 842 - 847, English

  [Refereed]

  Scientific journal


• Reactivation of thermally inactivated enzymes by free and immobilized chaperonin GroEL/ES

  T Teshima, A Kondo, H Fukuda

  Jul. 1997, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 48(1) (1), 41 - 46, English

  [Refereed]

  Scientific journal


• Operation conditions of enzyme refolding by chaperonin and recycle system using ultrafiltration

  Yoshiyuki Ishii, Tadanaru Teshima, Akihiko Kondo, Kaoru Murakami, Shuji Sonezaki, I. -Ogawa Hiroaki, Yasuhiko Kato, Hideki Fukuda

  Elsevier Science S.A., May 1997, Chemical Engineering Journal, 65(2) (2), 151 - 157, English

  [Refereed]

  Scientific journal


• Capabilities of polymer-modified monodisperse colloidal silica particles as biomaterial carrier

  Akihiko Kondo

  1997, Colloid and Polymer Science

  Scientific journal


• Production of glucoamylase by passively immobilized cells of a flocculent yeast, Saccharomyces diastaticus

  Hiroyuki Furuta, Terukazu Arai, Hideki Hama, Naofumi Shiomi, Akihiko Kondo, Hideki Fukuda

  Elsevier Sci B.V., 1997, Journal of Fermentation and Bioengineering, 84(2) (2), 169 - 171, English

  [Refereed]

  Scientific journal


• Use of a substrate as an encapsulated marker for liposome immunoassay

  Hideki Yamaji, Masato Nakagawa, Kanji Tomioka, Akihiko Kondo, Hideki Fukuda

  Elsevier Sci B.V., 1997, Journal of Fermentation and Bioengineering, 83(6) (6), 596 - 598, English

  [Refereed]

  Scientific journal


• Preparation of thermo-sensitive magnetic hydrogel microspheres and application to enzyme immobilization

  A Kondo, H Fukuda

  1997, JOURNAL OF FERMENTATION AND BIOENGINEERING, 84(4) (4), 337 - 341, English

  [Refereed]

  Scientific journal


• Adsorption activity and conformation of α-amylase on various ultrafine silica particles modified with polymer silane coupling agents

  Akihiko Kondo, Takafumi Urabe, Kohji Yoshinaga

  Elsevier Science B.V., Apr. 1996, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 109, 129 - 136, English

  [Refereed]

  Scientific journal


• Comparison of adsorption and conformation of hemoglobin and myoglobin on various inorganic ultrafine particles

  Akihiko Kondo, Jun Mihara

  Academic Press Inc., Jan. 1996, Journal of Colloid and Interface Science, 177(1) (1), 214 - 221, English

  [Refereed]

  Scientific journal


• Reaction properties of immobilized catalytic antibodies that deprotect acylated carbohydrates

  Akihiko Kondo, Hideki Fukuda, Yoshiharu Iwabuchi, Ikuo Fujii

  Society of Fermentation and Bioengineering, Japan, 1996, Journal of Fermentation and Bioengineering, 82(5) (5), 452 - 457, English

  [Refereed]

  Scientific journal


• Phospholipase D production using immobilized cells of Streptoverticillium cinnamoneum

  Hideki Fukuda, Yuji Turugida, Takahiro Nakajima, Eiji Nomura, Akihiko Kondo

  Kluwer Academic Publishers, 1996, Biotechnology Letters, 18(8) (8), 951 - 956, English

  [Refereed]

  Scientific journal


• Protein substrates and heat shock reduce the DNA-binding ability of Escherichia coli Lon protease

  S. Sonezaki, K. Okita, T. Oba, Y. Ishii, A. Kondo, Y. Kato

  Springer-Verlag, Dec. 1995, Applied Microbiology and Biotechnology, 44(3-4) (3-4), 484 - 488, English

  [Refereed]

  Scientific journal


• Relationship between molecular states (conformation and orientation) and activities of α-amylase adsorbed on ultrafine silica particles

  A. Kondo, T. Urabe

  Springer-Verlag, Oct. 1995, Applied Microbiology and Biotechnology, 43(5) (5), 801 - 807, English

  [Refereed]

  Scientific journal


• Temperature dependence of activity and conformational changes in α-amylases with different thermostability upon adsorption on ultrafine silica particles

  Akihiko Kondo, Takafumi Urabe

  Sep. 1995, Journal of Colloid And Interface Science, 174(1) (1), 191 - 198, English

  [Refereed]

  Scientific journal


• Efficient immobilization of protein on monodispersed colloidal silica particles modified by copolymers of maleic anhydride and styrene or methyl methacrylate

  Kohji Yoshinaga, Kimihiko Kondo, Akihiko Kondo

  1995, Polymer Journal, 27(1) (1), 98 - 100, English

  [Refereed]

  Scientific journal


• Preparation of immobilized enzyme with high activity using affinity tag based on proteins A and G

  Akihiko Kondo, Tadanaru Teshima

  1995, Biotechnology and Bioengineering, 46(5) (5), 421 - 428, English

  [Refereed]

  Scientific journal


• Overproduction and purification of SulA fusion protein in Escherichia coli and its degradation by Lon protease in vitro

  S. Sonezaki, Y. Ishii, K. Okita, T. Sugino, A. Kondo, Y. Kato

  1995, Applied Microbiology and Biotechnology, 43(2) (2), 304 - 309, English

  [Refereed]

  Scientific journal


• Overproduction and purification of Lon protease from Escherichia coli using a maltose-binding protein fusion system

  S. Sonezaki, A. Kondo, T. Oba, Y. Ishii, Y. Kato, H. Nakayama

  Springer-Verlag, Nov. 1994, Applied Microbiology and Biotechnology, 42(2-3) (2-3), 313 - 318, English

  [Refereed]

  Scientific journal


• Development and application of thermo-sensitive magnetic immunomicrospheres for antibody purification

  Akihiko Kondo, Hiroko Kamura, Ko Higashitani

  Springer-Verlag, Mar. 1994, Applied Microbiology and Biotechnology, 41(1) (1), 99 - 105, English

  [Refereed]

  Scientific journal


• Formation of Composite Latex Particles by Deposition of Amphoteric Particles

  Ko Higashitani, Takuya Yamamura, Yumiko Isshiki, Akiko Kage, Akihiko Kondo

  1994, KONA Powder and Particle Journal, 12, 119 - 124, English

  [Refereed]

  Scientific journal


• Development and application of thermo‐sensitive immunomicrospheres for antibody purification

  Akihiko Kondo, Tetsuya Kaneko, Ko Higashitani

  1994, Biotechnology and Bioengineering, 44(3) (3), 395 - 395, English

  [Refereed]

  Scientific journal


• Development and application of thermo‐sensitive immunomicrospheres for antibody purification

  Akihiko Kondo, Tetsuya Kaneko, Ko Higashitani

  1994, Biotechnology and Bioengineering, 44(1) (1), 1 - 6, English

  [Refereed]

  Scientific journal


• Bioconversions in an aqueous two-phase system using enzymes immobilized on ultrafine silica particles

  Akihiko Kondo, Takafumi Urabe, Ko Higashitani

  1994, Journal of Fermentation and Bioengineering, 77(6) (6), 700 - 703, English

  [Refereed]

  Scientific journal


• Preparation of immobilized papain using thermosensitive latex particles

  Akihiko Kondo, Kouichi Imura, Kouzo Nakama, Ko Higashitani

  1994, Journal of Fermentation and Bioengineering, 78(3) (3), 241 - 245, English

  [Refereed]

  Scientific journal


• Immunological agglutination behavior of latex particles with covalently immobilized antibodies

  Akihiko Kondo, Shuji Uchimura, Ko Higashitani

  1994, Journal of Fermentation and Bioengineering, 78(2) (2), 164 - 169, English

  [Refereed]

  Scientific journal


• Purification of fusion proteins using affinity microspheres in aqueous two-phase systems

  Akihiko Kondo, Tetsuya Kaneko, Ko Higashitani

  Springer-Verlag, Nov. 1993, Applied Microbiology and Biotechnology, 40(2-3) (2-3), 365 - 369, English

  [Refereed]

  Scientific journal


• Immobilization of protein on monodispersed colloidal silica with poly(ethylene glycol) spacer and application of the composites to immunological agglutination tests

  Kohji Yoshinaga, Akihiko Kondo, Ko Higashitani, Taketoshi Kito

  Sep. 1993, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 77(2) (2), 101 - 107, English

  [Refereed]

  Scientific journal


• Conformational changes in protein molecules upon adsorption on ultrafine particles

  Akihiko Kondo, Shinya Oku, Fumiyasu Murakami, Ko Higashitani

  Aug. 1993, Colloids and Surfaces B: Biointerfaces, 1(3) (3), 197 - 201, English

  [Refereed]

  Scientific journal


• Kinetic and circular dichroism studies of enzymes adsorbed on ultrafine silica particles

  Akihiko Kondo, Fumiyasu Murakami, Masako Kawagoe, Ko Higashitani

  Springer-Verlag, Aug. 1993, Applied Microbiology and Biotechnology, 39(6) (6), 726 - 731, English

  [Refereed]

  Scientific journal


• STABILITY OF ANTIBODY FRAGMENTS SECRETED TO THE CULTURE MEDICUM OF ESCHERICHIA-COLI

  Akihiko Kondo

  1993, Protein engineering

  Scientific journal


• Spectral Changes of Lysozyme Adsorbed on Ultrafine Silica Particles

  Hideto Matsuyama, Takashi Yamamoto, Setsuo Furuyoshi, Masaaki Teramoto, Akihiko Kondo

  1993, Bioscience, Biotechnology and Biochemistry, 57(6) (6), 992 - 993, English

  [Refereed]


• Formation of Composite Latex Particles by Deposition of Amphoteric Particles

  Ko Hierashitani, Takuya Yamamura, Yumiko Isshiki, Akiko Kage, Akihiko Kondo

  1992, KAGAKU KOGAKU RONBUNSHU, 18(5) (5), 637 - 642, English

  [Refereed]

  Scientific journal


• Effect of Copolymer Composition on γ-Globulin Adsorption and Covalent Immobilization onto Microspheres

  Akihiko Kondo, Katsuhiro Yoshioka, Ko Higashitani

  1992, KAGAKU KOGAKU RONBUNSHU, 18(3) (3), 353 - 359, English

  [Refereed]

  Scientific journal


• Adsorption of model proteins with wide variation in molecular properties on colloidal particles

  Akihiko Kondo, Ko Higashitani

  1992, Journal of Colloid And Interface Science, 150(2) (2), 344 - 351, English

  [Refereed]

  Scientific journal


• Affinity purification of antibodies using immunomicrospheres

  Akihiko Kondo, Ryo Yamasaki, Ko Higashitani

  1992, Journal of Fermentation and Bioengineering, 74(4) (4), 226 - 229, English

  [Refereed]

  Scientific journal


• Dispersion of coagulated particles by contractile flow to orifice

  Ko Higashitani, Nobuhisa Tanise, Atsuro Yoshiba, Akihiko Kondo

  1992, Journal of Chemical Engineering of Japan, 25(5) (5), 502 - 507, English

  [Refereed]

  Scientific journal


• Circular dichroism studies on conformational changes in protein molecules upon adsorption on ultrafine polystyrene particles

  Akihiko Kondo, Fumiyasu Murakami, Ko Higashitani

  1992, Biotechnology and Bioengineering, 40(8) (8), 889 - 894, English

  [Refereed]

  Scientific journal


• Immunological agglutination kinetics of latex particles with covalently immobilized antigens

  Akihiko Kondo, Takeharu Kawano, Ko Higashitani

  1992, Journal of Fermentation and Bioengineering, 73(6) (6), 435 - 439, English

  [Refereed]

  Scientific journal


• Structural changes in protein molecules adsorbed on ultrafine silica particles

  Akihiko Kondo, Shinya Oku, Ko Higashitani

  1991, Journal of Colloid And Interface Science, 143(1) (1), 214 - 221, English

  [Refereed]

  Scientific journal


• Engineering proteins to enhance their partition coefficients in aqueous two-phase systems

  Kristina Köhler, Charlotte Ljungquist, Akihiko Kondo, Andres Veide, Björn Nilsson

  Presse Dienstleistungsgesellschaft mbH und Co. KG, 1991, Nature Biotechnology, 9(7) (7), 642 - 646, English

  [Refereed]

  Scientific journal


• Effects of peptide antigenic determinant properties on adsorption equilibrium of anti-peptide antibodies

  Akihiko Kondo, Shinichi Furukawa, Masato Taira, Ko Higashitani

  1991, Journal of Fermentation and Bioengineering, 72(6) (6), 409 - 412, English

  [Refereed]

  Scientific journal


• Adsorption of γ‐globulin, a model protein for antibody, on colloidal particles

  Akihiko Kondo, Shinya Oku, Ko Higashitani

  1991, Biotechnology and Bioengineering, 37(6) (6), 537 - 543, English

  [Refereed]

  Scientific journal


• Engineering proteins to enhance their partition coefficients in aqueous two-phase systems

  Kristina Köhler, Charlotta Ljungquist, Akihiko Kondo, Andres Veide, Björn Nilsson

  1991, Bio/Technology, 9(7) (7), 642 - 646, English

  [Refereed]

  Scientific journal


• Immunological agglutination kinetics of latex particles with physically adsorbed antigens

  Akihiko Kondo, Takeharu Kawano, Fumio Itoh, Ko Higashitani

  Dec. 1990, Journal of Immunological Methods, 135(1-2) (1-2), 111 - 119, English

  [Refereed]

  Scientific journal


• Characterization of the Immunointeraction and Its Application to Bioaffinity Separation

  EIZO SADA, SHIGEO KATOH, AKIHIKO KONDO, YOSHINORI SOHMA

  1990, Annals of the New York Academy of Sciences, 613(1) (1), 874 - 877, English

  [Refereed]

  Scientific journal


• Adsorption equilibrium in immunoaffnity chromatography with antibodies to synthetic peptides

  Akihiko Kondo, Hiroyuki Takamatsu, Shigeo Katoh, Eizo Sada

  1990, Biotechnology and Bioengineering, 35(2) (2), 146 - 151, English

  [Refereed]

  Scientific journal


• Improvement of proteolytic resistance of immunoadsorbents by chemical modification with polyethylene glycol

  Akihiko Kondo, Masaaki Kishimura, Shigeo Katoh, Eizo Sada

  1989, Biotechnology and Bioengineering, 34(4) (4), 532 - 540, English

  [Refereed]

  Scientific journal


• Effect of histidine residues in antigenic sites on pH dependence of immuno-adsorption equilibrium

  Eizo Sada, Shigeo Katoh, Atsuo Kiyokawa, Akihiko Kondo

  Springer-Verlag, Feb. 1988, Applied Microbiology and Biotechnology, 27(5-6) (5-6), 528 - 532, English

  [Refereed]

  Scientific journal


• Effects of charged groups in haptens on adsorption equilibrium of hapten antibody

  Eizo Sada, Shigeo Katoh, Hirotoshi Miyoshi, Kuniko Yamanaka, Akihiko Kondo

  1988, Biotechnology and Bioengineering, 32(4) (4), 467 - 474, English

  [Refereed]

  Scientific journal


• Characterization of fractionated polyclonal antibodies for immunoaffinity chromatography

  Eizo Sada, Shigeo Katoh, Atsuo Kiyokawa, Akihiko Kondo

  1988, Biotechnology and Bioengineering, 31(7) (7), 635 - 642, English

  [Refereed]

  Scientific journal


• HETP values of large-scale columns for liquid chromatography

  Eizo Sada, Shigeo Katoh, Akihiko Kondo, Seiji Ishida

  1987, Journal of Fermentation Technology, 65(1) (1), 111 - 113, English

  [Refereed]

  Scientific journal


• Effects of coupling method and ligand concentration on adsorption equilibrium in immuno-affinity chromatography

  Eizo Sada, Shigeo Katoh, Akihiko Kondo, Atsuo Kiyokawa

  1986, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 19(6) (6), 502 - 506, English

  [Refereed]

  Scientific journal


• Adsorption equilibrium in immuno‐affinity chromatography with polyclonal and monoclonal antibodies

  Eizo Sada, Shigeo Katoh, Kiyoshi Sukai, Masaaki Tohma, Akihiko Kondo

  1986, Biotechnology and Bioengineering, 28(10) (10), 1497 - 1502, English

  [Refereed]

  Scientific journal