Research activity information

• Efficient and high-density immobilization of animal cells by a microfiber with both swelling and cell adhesion properties and its application to exosome production

  Naofumi Shiomi, Pengfei Zhang, Shuji Nakatsuka, Kazuo Kumagai, Hideto Matsuyama

  Abstract Purpose For high-density cell culture, we studied the development of optimal microfibers (MFs) with a 0.1–10 μm diameter, which due to their large surface area can serve as an immobilization carrier for animal cells. To date, few studies have used MFs as scaffolding for high-density cell culturing. Results Using six types of nonsoluble synthetic polymers, MF sheets were fabricated by electrospinning. The cellulose acetate, polyketone, and polyvinyl acetate MFs exhibited swelling and water retention capacities. Next, the six types of MF fragments were examined for immobilizing TKD2 mouse vascular endothelial cells. Although most cells were taken into the three MFs characterized by swelling, most leaked from the MFs without adhesion. To solve this, the MF sheets comprising cellulose acetate and polyketones were coated with gelatin. Although the adhesive capacity was enhanced, the swelling capacity decreased and almost all the immobilized mouse cells remained on the sheets’ surfaces. Based on these results, we produced a novel MF sheet comprising a gelatin, cellulose acetate, and polyketone mixture (CPG). Since the cells were taken into the MFs by swelling and attached by the gelatin, the CPG fragment immobilized almost all the supplied cells with little loss and reached a high density of 3.2 × 10⁹ MF-g⁻¹, Furthermore, the immobilized cells continuously produced exosomes with a high productivity of 6–7 × 10¹⁰ particles ml⁻¹ after either 8 h or 16 h of culturing. Conclusion CPG-based MFs are expected to have a wide range of future applications, including exosome production from animal cells.

  Springer Science and Business Media LLC, Apr. 2025, Biotechnology Letters, 47(2) (2), English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Direct Correlation among Telomere Length, Cellular Aging, and Rejuvenation Effects of Honey Child Powder

  Naofumi Shiomi, Keiko Watanabe, Yuki Fujiwara, Takae Yamasaki, Hideto Matsuyama

  Scientific Research Publishing, Inc., 2024, Journal of Biosciences and Medicines, 12(07) (07), 55 - 70, English

  [Refereed]

  Scientific journal


• High concentration of spermine induces the dedifferenytiation of somatic cells into pluripotent stem cells

  SHIOMI Naofumi

  Feb. 2019, Journal of Biomedical Science and Engineering, 12(2) (2), 92 - 110, English

  [Refereed]

  Scientific journal


• Characteristics of beige adipocytes induced from white adipocytes by kikyo extract

  SHIOMI Naofumi

  Jun. 2016, Journal of Biomedical Science and Engineering, 9, 342 - 353, English

  [Refereed]

  Scientific journal


• Effects of hydrocortisone, glycerophosphate and retinol on the differentiation of mesnchumal stem cells and vasclar endothelial cells to osteoblasts

  SHIOMI Naofumi, Keiko Watanabe

  Nov. 2014, Journal of Biomedical Science and Engineering, 19(13) (13), 1056 - 1066, English

  [Refereed]

  Scientific journal


• Effect of oleic acid on murine macrophage dysfunction

  SHIOMI Naofumi, Keiko Watanabe

  2013, Journal of Biomedical Science and Engineering, 6(6) (6), 654 - 660, English

  [Refereed]

  Scientific journal


• Biodegradation of Melamine and Cyanuric Acid by a Newly-Isolated Microbacterium Strain

  Naofumi Shiomi, Maiko Ako

  2012, Advances in Microbiology, 2(3) (3), 303 - 309, English

  [Refereed]

  Scientific journal


• Glutathione Production by Yarrowia lipolytica Showing Both Methyglyoxal Resistance and a High Activity of Glutathione Synthetase

  Naofumi Shiomi, Emi Harada, Risako Nabeshima

  2012, Advances in Microbiology, 2(2) (2), 171 - 180, English

  [Refereed]

  Scientific journal


• Compounds that inhibit triglyceride accumulation and TNFα secretion in adipocytes

  Naofumi Shiomi, Miwako Maeda, Minori Mimura

  2011, Journal of Biomedical Science and Engineering, 4(11) (11), 684 - 691, English

  [Refereed]

  Scientific journal


• Mechanism of metabolic syndrome and its revention

  Miwako Maeda, Naofumi Shiomi

  神戸女学院大学大学院人間科学研究科, 2010, Human Science, 13(13) (13), 72 - 75, Japanese

  Research institution


• Oil degradation in soil by a hydrocarbon assimilating yeast, Yarrowia lipolytica

  Mayu Fukui, Shinji Shiomi, Naoki Tanuma, Tomohisa Katsuda, Naofumi Shiomi, Hideki Yamaji

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(1) (1), S93 - S94, English

  [Refereed]

  International conference proceedings


• A highly sensitive enzyme-linked immunosorbent assay for quantification of adipocytokines secreted by mouse adipocytes

  Minori Mimura, Risako Nabeshima, Miwako Maeda, Naofumi Shiomi

  Jan. 2009, BIOCHEMICAL ENGINEERING JOURNAL, 43(1) (1), 58 - 63, English

  [Refereed]

  Scientific journal


• Neutralization of acidified water by use of Rhizopus delemar immobilized with a cellulose membrane

  Risako Nabeshima, Noriko Kusumoto, Shigeo Katoh, Naofumi Shiomi

  Sep. 2008, BIOCHEMICAL ENGINEERING JOURNAL, 41(2) (2), 188 - 192, English

  [Refereed]

  Scientific journal


• Production of Single-Chain Variable Fragment Antibody (scFv) in Fed-Batch and Continuous Culture of Pichia pastoris by Two Different Methanol Feeding Methods

  Shinya Yamawaki, Takehiro Matsumoto, Yuka Ohnishi, Yoichi Kumada, Naofumi Shiomi, Tomohisa Katsuda, Eun Kyu Lee, Shigeo Katoh

  Nov. 2007, Journal of Bioscience and Bioengineering, 104(5) (5), 403 - 407, English

  [Refereed]

  Scientific journal


• Characteristic of tryglyceride accumulation and production of adipocytokines in mouse adipocyte

  N. Shiomi, M. Mimura, R. Nabesima

  2007, Extended abstract APBioChEC'07, A0027, 1 - 4, English

  [Refereed]


• Degradation of cyanuric acid in soil by Pseudomonas sp NRRL B-12227 using bioremediation with self-immobilization system

  Naofumi Shiomi, Yutaka Yamaguchi, Hiroaki Nakai, Tomoko Fujita, Tomohisa Katsuda, Shigeo Katoh

  Sep. 2006, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102(3) (3), 206 - 209, English

  [Refereed]

  Scientific journal


• Effects of methanol feeding methods on chimeric alpha-amylase expression in continuous culture of Pichia pastoris

  A Nakano, CY Lee, A Yoshida, T Matsumoto, N Shiomi, S Katoh

  Mar. 2006, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 101(3) (3), 227 - 231, English

  [Refereed]

  Scientific journal


• Bioremediation of soil and ponds acidified by acid rain with fungi cells

  N. Shiomi, N. Kusumoto, R. Nabeshima, T. Yasuda, S N. Kusumoto, R. Nabeshima, T. Yasuda, S. Iwasaki, Y.Yamaguchi, S. Katoh

  2005, Extended abstract APBioChEC'05, P1-015, 1 - 4, English

  [Refereed]


• Neutralization of acids by microorganisms co-immobilized with Bacillus subtilis in a shallow layer of model soil

  T Yasuda, N Shiomi, S Iwasaki, Y Yamaguchi, T Katsuda, S Katoh

  Dec. 2004, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 37(12) (12), 1445 - 1451, English

  [Refereed]

  Scientific journal


• Bioremediation Process for soil using microbiology

  Naofumi Shiomi, Shigeo Katoh

  2004, Chemical Engineering, 40(5) (5), 368 - 372, Japanese

  [Invited]

  Scientific journal


• Characteristics of neutralization of acids by newly isolated fungal cells

  Naofumi Shiomi, Takako Yasuda, Yoko Inoue, Noriko Kusumoto, Saori Iwasaki, Tomohisa Katsuda, Shigeo Katoh

  Lead, Jan. 2004, J Biosci Bioeng, 97(1) (1), 54 - 58, English

  [Refereed]

  Scientific journal


• Effects of substrate feed rates on heterologous protein expression by Pichia pastoris in DO-stat fed-batch fermentation

  CY Lee, A Nakano, N Shiomi, EK Lee, S Katoh

  Sep. 2003, ENZYME AND MICROBIAL TECHNOLOGY, 33(4) (4), 358 - 365, English

  [Refereed]

  Scientific journal


• 前発酵の冷凍耐性を維持するパン酵母の変異株と冷凍パン生地への利用

  塩見尚史, 安田多賀子

  Jun. 2003, 日本食品工学会誌, 4(2) (2), 53 - 60

  [Refereed]


• A bioremediation method based on self-immobilization of cells in shallow layer of soil

  T Yasuda, S Katoh, Y Inoue, N Shiomi

  Feb. 2003, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 36(2) (2), 216 - 219, English

  [Refereed]

  Scientific journal


• Immobilization of Candida cells showing mycelium-like shapes in porous polyvinyl formal resin and their applications

  N Shiomi, Y Inoue, K Tomioka, T Yasuda

  Feb. 2003, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 36(2) (2), 161 - 165, English

  [Refereed]

  Scientific journal


• Secretion of alpha-amylase from Saccharomyces cerevisiae and Pichia pastoris and characterization of its C-terminus with an anti-peptide antibody

  T Kurokawa, C Lee, N Shiomi, A Nakano, S Katoh

  Dec. 2002, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 35(12) (12), 1277 - 1281, English

  [Refereed]

  Scientific journal


• A new method for production of combinatorial libraries by mating of Saccharomyces cerevisiae cells

  N Shiomi, K Murao, H Koga, K Kuroda, H Hosokawa, S Katoh

  May 2002, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 35(5) (5), 474 - 478, English

  [Refereed]

  Scientific journal


• 固定化Candida酵母による油類に汚染された海水の修復

  井上洋子, 塩見尚史

  神戸女学院大学大学院人間科学研究科, 2002, ヒューマンサイエンス, 5(5) (5), 27 - 34, Japanese

  Scientific journal


• Adsorption and elution characteristics of anti-peptide antibodies in immunoaffinity chromatography

  S. Katoh, K. Murao, N. Hori, M. Terashima, N. Shiomi

  Aug. 2001, Sientica Iranica, 8(3) (3), 166 - 170, English

  [Refereed]


• Effective bioremediation process by using the flocclemce of Bacillus cells

  Yoko Inoue, Naofumi Shiomi

  Mar. 2001, Human Science, (4) (4), 1 - 8, Japanese

  Research institution


• 前発酵後も冷凍耐性を維持できる酵母の育種とその利用

  塩見尚史

  Lead, May 2000, エリザベスアーノルド富士財団 平成12年度報告書, 208 - 214, Japanese

  Research society


• Production of a human IgM-type antibody and preparation of combinatorial library by recombinant Saccharomyces cerevisiae

  N Shiomi, K Murao, H Koga, S Katoh

  Mar. 2000, BIOSEPARATION ENGINEERING, 16, 75 - 79, English

  [Refereed]

  International conference proceedings


• Immobilization of Candida yeasts with porous polyvinyl formal resin and their application.

  N.Shiomi, S. Katoh

  Nov. 1999, Symposium series of Chemical Engineering "Biochmical Engineering and Bioseparation Engineering", 70, 95 - 99, English

  [Refereed]


• 前発酵後も冷凍耐性を維持できる酵母の育種

  塩見尚史

  May 1999, エリザベスアーノルド富士財団 平成11年度報告書, 208 - 214, Japanese

  Research society


• Utilization of antipeptide antibodies as affinity ligands in immunoaffinity purification

  Shigeo Katoh, Masaaki Terashima, Naofumi Shiomi

  Sep. 1998, Journal of Chromatography B: Biomedical Applications, 715(1) (1), 147 - 152, English

  [Refereed]

  Scientific journal


• Production and purification of soluble human CD2 secreted from recombinant Pichia pastoris

  N Shiomi, K Tomioka, S Katoh

  Mar. 1998, PROCESS BIOCHEMISTRY, 33(4) (4), 377 - 383, English

  [Refereed]

  Scientific journal


• Expression and characterization of (R)-specific enoyl coenzyme A hydratase involved in polyhydroxyalkanoate biosynthesis by Aeromonas caviae

  T Fukui, N Shiomi, Y Doi

  Feb. 1998, JOURNAL OF BACTERIOLOGY, 180(3) (3), 667 - 673, English

  [Refereed]

  Scientific journal


• Production and purification of soluble human CD2 secreted from recombinant Pichia pastoris

  N. Shiomi, K. Tomioka, S. Katoh

  1998, Process Biochemistry, 33(4) (4), 377 - 383, English

  [Refereed]


• Production and purification of solbule human CD2 secreted from recombinant Pichia pastoris

  N. Shiomi, Y. Tabata, K. Okamoto, K. Murao, S. Y. Tabata, K. Okamoto, K. Murao, S. Katoh

  1998, Chemical Engineering series of Symposium 65, Advances in Bioseparation Engineering 1996-1997, 170 - 174, English

  [Refereed]

  Scientific journal


• Production of glucoamylase by passively immobilized cells of a flocculent yeast, Saccharomyces diastaticus

  H Furuta, T Arai, H Hama, N Shiomi, A Kondo, H Fukuda

  Oct. 1997, JOURNAL OF FERMENTATION AND BIOENGINEERING, 84(2) (2), 169 - 171, English

  [Refereed]

  Scientific journal


• Immunogenicity differences between intact insulin and a peptide representing the C-terminal region

  Norihiro Hikida, Kanji Tomioka, Naofumi Shiomi, Masaaki Terashima, Shigeo Katoh

  Elsevier Science S.A., Oct. 1997, Chemical Engineering Journal, 65(3) (3), 257 - 261, English

  [Refereed]

  Scientific journal


• Immunogenicity differences between intact insulin and a peptide representing the C-terminal region

  N Hikida, K Tomioka, N Shiomi, M Terashima, S Katoh

  Oct. 1997, CHEMICAL ENGINEERING JOURNAL, 65(3) (3), 257 - 261, English

  [Refereed]

  Scientific journal


• Purification of recombinant protein by anti-peptide antibodies

  Sigeo Katoh, Masaaki Terashima, Naofumi Shiomi

  Jul. 1997, Proceeding if the 4th Asia-Pasific Biochemical Engineering Conference, 875 - 878


• Improvement of S-adenosyl-L-methionine production by integration of the ethionine resistance gene into chromosomes of yeast Saccharomyces cerevisiae

  N SHIOMI, H FUKUDA, K MURATA, A KIMURA

  Dec. 1995, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 42(5) (5), 730 - 733, English

  [Refereed]


• Basic study on the production of S-adenosylmethiomine by the recombinant yeast

  SHIOMI Naofumi

  Mar. 1992, Kyoto University, Japanese, No password

  [Refereed]

  Doctoral thesis

  添付ファイルのURL


• Production of S-adenosyl-D, L-homocystein by Saccharomyces cerevisiae cells transformed with an ethionine resistance gene

  N. Shiomi, H. Fukuda, Y. Fukuda, K. Murata, A. Kimura

  Jun. 1991, Agricultural and Biological Chemistry, 71(4) (4), 211 - 215

  [Refereed]


• Nucleotide sequence and characterization of a gene conferring resistance to ethionine in yeast Saccharomyces cerevisiae

  N SHIOMI, H FUKUDA, Y FUKUDA, K MURATA, A KIMURA

  Apr. 1991, JOURNAL OF FERMENTATION AND BIOENGINEERING, 71(4) (4), 211 - 215, English

  [Refereed]

  Scientific journal


• EXPRESSION AND STABILITY OF PLASMID WITH ETHIONINE RESISTANCE GENE AS A MARKER IN SACCHAROMYCES-CEREVISIAE

  N SHIOMI, H FUKUDA, Y FUKUDA, K MURATA, A KIMURA

  Mar. 1991, KAGAKU KOGAKU RONBUNSHU, 17(3) (3), 674 - 679, Japanese

  [Refereed]

  Scientific journal


• Production of S-adenosyl-L-methionine by Saccharomyces cerevisiae cells carrying a gene for ethionine resistance

  N SHIOMI, H FUKUDA, Y FUKUDA, K MURATA, A KIMURA

  May 1990, BIOTECHNOLOGY AND BIOENGINEERING, 35(11) (11), 1120 - 1124, English

  [Refereed]

  Scientific journal


• Cloning of genes for spermine resistance in Saccharomyces cerevisiae and thier effects on S-adenosyl-L-methionine accumulation

  N SHIOMI, H FUKUDA, Y FUKUDA, K MURATA, A KIMURA

  Jan. 1990, JOURNAL OF FERMENTATION AND BIOENGINEERING, 69(1) (1), 63 - 66, English

  [Refereed]

  Scientific journal


• Cloning of a gene for s-adenosylmethionine synthesis in Saccharomyces cerevisiae

  N SHIOMI, H FUKUDA, H MORIKAWA, Y FUKUDA, A KIMURA

  Sep. 1988, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 29(2-3) (2-3), 302 - 304, English

  [Refereed]


• Separation of metals by liquid membrane with tri-n-octylamine

  M HARADA, N SHIOMI, F YAMAZAKI

  Dec. 1984, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 17(6) (6), 567 - 572, English

  [Refereed]

  Scientific journal


• Selective separation of metals by liquid membrane with mixed carriers

  M HARADA, N SHIOMI

  Dec. 1984, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 17(6) (6), 572 - 576, English

  [Refereed]

  Scientific journal


• Rate of ferric chloride extraction with tri-n-octyamine

  M HARADA, F YAMAZAKI, N SHIOMI, M ADACHI, W EGUCHI

  Oct. 1984, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 17(5) (5), 521 - 527, English

  [Refereed]

  Scientific journal


• Separation of heavy metals by liquid membrane containing mixed carrier

  Makoto Harada

  1984, Chemical Engineering series of symosium 5, 133 - 137, Japanese

  Scientific journal


• Study on the separation ablility by liquid membrane using amine-based high molecular compounds

  SHIOMI Naofumi

  Mar. 1983, School of Eniginnering, Kyoto University, Japanese

  [Refereed]

  Master thesis


• Solubility of carbon dioxide in molten alkali halides and nitrates and their binary mixtures

  Eizo Sada, Shigeo Katoh, Hidefumi Yoshii, Ikuo Tekemoto, Naofumi Shiomi

  1981, JOURNAL OF CHEMICAL AND ENGINEERING DATA, 26(3) (3), 279 - 281, English

  [Refereed]

  Scientific journal

• Introduction of my research

  SHIOMI Naofumi

  Apr. 2016, Kobe College, 177, 49, Japanese

  Others


• 3P-092 Effects of telomere length on the rejuvenation of cell

  Shiomi Naofumi, Watanabe Keiko

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 293 - 293, Japanese


• 出会えたことを神様に感謝

  塩見 尚史

  Mar. 2013, 『VERITUS』 神戸女学院図書館, 49, Japanese

  Book review


• Production of glutathione by using Yarrowia lipolytica enhanced the activity of glutathione synthetase

  Naofumi Shiomi, Emi Harada, Risako Nabeshima

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S124 - S124, English

  Summary international conference


• Effect of carboxylates on triglyceride accumulation and a TNF alpha production in mouse cells

  Maeda Miwako, Mimura Minori, Shiomi Naofumi

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S34 - S34, English

  Summary international conference


• 1I09-2 Characteristics of accumulation of triglyceride and metabolic rate of glucose in mouse hepatocyte

  NABESHIMA Risako, MIMURA Minori, SHIOMI Naofumi

  日本生物工学会, 2007, 日本生物工学会大会講演要旨集, 19, 189 - 189, Japanese


• 1I09-1 Characteristics of production of tryglyceride and adipocytokine in mouse adipocyte

  SHIOMI Naofumi, MIMURA Minori, NABESHIMA Risako

  日本生物工学会, 2007, 日本生物工学会大会講演要旨集, 19, 189 - 189, Japanese


• 3L10-3 Single-chain Fv production by continuous culture of methy-lotrophic yeast Pichia pastoris

  YAMAWAKI Shinya, MATSUMOTO Takehiro, ONISHI Yuka, KATSUDA Tomohisa, SHIOMI Naofumi, KATOH Shigeo

  日本生物工学会, 2006, 日本生物工学会大会講演要旨集, 18, 226 - 226, Japanese


• 2B16-1 Bioremediation of ponds acidified by acid rain with fungi cells

  SHIOMI Naofumi, NABESHIMA Risako, KUSUMOTO Noriko, YASUDA Takako, KATOH Shigeo

  日本生物工学会, 2005, 日本生物工学会大会講演要旨集, 17, 89 - 89, Japanese


• 2B14-2 Bioremediation of herbicide-polluted soil with self-immobilized microorganisms.

  NAKAI Hiroaki, YAMAGUCHI Yutaka, YASUDA Takako, ARII Kenji, SAKAI Toyohide, KATSUDA Tomohisa, KATOH Shigeo, SHIOMI Naofumi

  日本生物工学会, 2005, 日本生物工学会大会講演要旨集, 17, 87 - 87, Japanese


• 2B12-2 Characteristics of degradation of melamine with bacterial cells isolated from soil

  URASE Maiko, SHIOMI Naofumi, NAKANO Mayu, HIRATA Eri, NABESHIMA Risako, KATOH Shigeo

  日本生物工学会, 2005, 日本生物工学会大会講演要旨集, 17, 86 - 86, Japanese


• 3E10-2 Study on Effective Production of Single-Chain Antibody by Methylotrophic Yeast

  MATSUMOTO Takehiro, YAMAWAKI Shinnya, ONISHI Yuka, SHIOMI Naohumi, KATOH Shigeo

  日本生物工学会, 2005, 日本生物工学会大会講演要旨集, 17, 160 - 160, Japanese


• 微生物を利用した土壌汚染浄化法 (特集 土壌汚染の防止技術と対策)

  塩見 尚史, 加藤 滋雄

  化学工業社, May 2004, ケミカルエンジニヤリング, 49(5) (5), 368 - 373, Japanese


• 2E16-3 Decontamination of soil polluted with herbicide by BSIS

  YAMAGUCHI Yutaka, NAKAI Hiroaki, FUJITA Tomoko, SHIOMI Naofumi, KATOH Shigeo

  日本生物工学会, 2004, 日本生物工学会大会講演要旨集, 16, 173 - 173, Japanese


• Effective methanol feeding strategy in continuous culture of Pichia pastoris

  Nakano Atsushi, Lee Chunyeon, Yoshida Arei, Matsumoto Takehiro, Shiomi Naohumi, Katoh Shigeo

  日本生物工学会, 2003, 日本生物工学会大会講演要旨集, 15, 115 - 115, Japanese


• Effective Neutralization of Acid Soil by BSIS

  Iwasaki Saori, Yasuda Takako, Yamaguchi Yutaka, Nakai Hiroaki, Shiomi Naofumi, Katoh Shigeo

  日本生物工学会, 2003, 日本生物工学会大会講演要旨集, 15, 215 - 215, Japanese


• 908 Screening of microorganisms secreting bases and application to neutrarization of acid rain

  Yasuda Takako, Iwasaki Saori, Yamaguchi Yutaka, Inoue Yoko, Shiomi Naofumi, Katoh Shigeo

  日本生物工学会, 2002, 日本生物工学会大会講演要旨集, 14, 166 - 166, Japanese


• Production of human IgM-type antibody and preparation of combinantorial library by recombinant Saccharomyces cerevisiae

  Shiomi Naofumi, Murao Kenji, Koga Hirohisa, Furuta Motoyuki, Katoh Shigeo

  日本生物工学会, 1999, 日本生物工学会大会講演要旨集, 11, 140 - 140, Japanese


• Utilization of antipeptide antibodies as affinity ligands in immunoaffinity purification

  KATOH Shigeo, TERASHIMA Masaaki, SHIOMI Naofumi

  11 Sep. 1998, Journal of chromatography. B, Biomedical sciences and applications, 715(1) (1), 147 - 152, English


• Improvement of S-adenosylmethionine production by yeast cells carrying ethionine-resistant gene

  Shiomi N., Fukuda H., Fukuda Y., Murata K., Kimura A.

  日本醗酵工学会, 1991, 日本醗酵工学会大会講演要旨集, 3, 226 - 226, Japanese


• PRODUCTION OF S-ADENOSYL-D,L-HOMOCYSTEINE BY SACCHAROMYCES-CEREVISIAE CELLS TRANSFORMED WITH AN ETHIONINE RESISTANCE GENE

  N SHIOMI, H FUKUDA, Y FUKUDA, K MURATA, A KIMURA

  Jun. 1990, AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 54(6) (6), 1595 - 1596, English

  Introduction scientific journal


• Biochemical and genetic analyses of ethionine resistance gene in ___- ___-

  Shiomi N., Fukuda H., Fukuda Y., Murata K., Kimura A.

  日本醗酵工学会, 1990, 日本醗酵工学会大会講演要旨集, 2, 6 - 6, Japanese


• Cloning spermine resistance gene in Saccharomyces cerevisiae and production of S-adenosyl-L-methionine Kanegafuchi Chemical Industry

  Shiomi Naofumi, Fukuda Hideki, Fukuda Yasuki, Murata Kousaku, Kimura Akira

  日本醗酵工学会, 1989, 日本醗酵工学会大会講演要旨集, 175 - 175, Japanese

• The strategy and future of biotechnology in protecting the global environment

  Single work, All, InTech publisher, Dec. 2023


• Bioremediation for Global Environmental Conservation

  Naofumi Shiomi, Zambale a, Dr. Md Dinh

  Joint editor, All, InTech publisher, Dec. 2023

  [Refereed]


• Introductory Chapter: An Overview of Metabolic Syndrome and Its Prevention

  Naofumi Shiomi

  Single work, All, InTech, Dec. 2022, 11, English, Lifestyle-related diseases are caused by an unbalanced diet and irregular, undesirable lifestyle, leading to a condition called metabolic syndrome. Rather like dominoes falling, these diseases develop one after another. Owing to changes in dietary habits and working environments, the incidence of lifestyle-related diseases, obesity, and metabolic syndrome is increasing globally, resulting in a serious social problem that needs to be solved. This book deals with recent studies on lifestyle-related diseases and metabolic syndrome, and the following three topics are discussed: (1) characteristics of metabolic syndrome and lifestyle-related diseases; (2) the relationship between metabolic syndrome and several diseases; and (3) effective interventions to prevent lifestyle-related diseases. This book provides new ideas for the treatment and prevention of metabolic syndrome and lifestyle-related diseases.

  [Refereed]

  Scholarly book

  DOI


• Lifestyle-Related Diseases and Metabolic Syndrome

  Editor, All, InTech, 2022, 160, English, Lifestyle-related diseases are caused by an unbalanced diet and irregular, undesirable lifestyle, leading to a condition called metabolic syndrome. Rather like dominoes falling, these diseases develop one after another. Owing to changes in dietary habits and working environments, the incidence of lifestyle-related diseases, obesity, and metabolic syndrome is increasing globally, resulting in a serious social problem that needs to be solved. This book deals with recent studies on lifestyle-related diseases and metabolic syndrome, and the following three topics are discussed: (1) characteristics of metabolic syndrome and lifestyle-related diseases; (2) the relationship between metabolic syndrome and several diseases; and (3) effective interventions to prevent lifestyle-related diseases. This book provides new ideas for the treatment and prevention of metabolic syndrome and lifestyle-related diseases., ISBN: 9781803567808

  [Refereed]


• Current Topics in Functional Food

  Editor, All, InTech, 2022, English, The market for functional foods is steadily expanding as more people worldwide realize the value of the daily consumption of healthy foods in maintaining good health. Recent studies have revealed new functional compounds in foods. Genetically modified foods will soon be commercially available. This book discusses the characteristics of functional foods and the health benefits of ingredients including ginger, herbs, probiotics, mushrooms, and dairy products. It also provides new ideas for the production of new functional foods and managing health through the daily diet., ISBN: 9781803557939

  [Refereed]

  Scholarly book

  DOI


• Introduction to biotechnology for next generation

  Naofumi Shiomi, Akifumi Shiomi

  Joint work, Daigaku-kyoiku, Nov. 2021, Japanese, ISBN: 9784866921662

  Textbook


• 生命科学が解き明かす食と健康

  塩見尚史・塩見晃史

  Joint work, 大学教育出版, Apr. 2020, Japanese, ISBN: 9784866920726

  Textbook


• アートウォークinKobeー神戸のパブリックアート散策ー

  塩見尚史

  Single work, 三和印刷（株）, Mar. 2020, Japanese, 神戸にあるパブリックアートを散策するためのガイドブック

  Others


• Current Topics in Biochemical Engineering

  SHIOMI Naofumi

  Editor, All, InTech, Aug. 2019


• Introductory Chapter: Artificial Enzyme Introduced by Directed Evolution Technology

  SHIOMI Naofumi

  Single work, Current Topics in Biochmical Engineering (chapter 1), InTech, Aug. 2019, English

  [Refereed]

  Scholarly book

  DOI


• Introductory Chapter: Design of an Ideal Diet Using Common Foods

  SHIOMI Naofumi

  Single work, Current Topics of Superfoods (Chapter1), InTech, May 2018, 13, English

  [Refereed]

  Scholarly book

  DOI


• Current Topics of Superfoods

  SHIOMI Naofumi

  Editor, All, InTEch, May 2018


• Diabetes Mellitus in South Asia

  SHIOMI Naofumi

  Joint work, Diabetes and Its Complications (chapter 1), InTech piblisher, Apr. 2018

  [Refereed]


• Introductory Chapter: Serious Pollution of Soil and Groundwater and the Necessity of Bioremediation

  SHIOMI Naofumi

  Contributor, Advances in Bioremediation and Phytoremediation (Chapter 1), InTech, Apr. 2018

  [Refereed]


• Advances in Bioremediation and Phytoremediation

  SHIOMI Naofumi

  Editor, InTech, Apr. 2018, English, ISBN: 9789535139584

  Scholarly book


• Secret of Body Elucidated by Life Science (Japanese)

  SHIOMI Naofumi

  Joint work, Daigaku kyouiku shuppan, Jun. 2017


• Superfood and Functional Food-An overview of Thier Procesing and Utilization

  Viduranga Waisundara, SHIOMI Naofumi

  Joint editor, InTech, Mar. 2017


• Superfood and Functional Food-The Development of Superfoods and Their Roles as Medicine

  SHIOMI Naofumi, Viduranga Waisundara

  Editor, InTech, Feb. 2017


• Introductory Chapter: Recent Studies on Cellular Aging and Rejuvenation( in Molecular Mechanisms of the Aging Process and Rejuvenation)

  SHIOMI Naofumi

  Contributor, Chapter 1, InTech, Aug. 2016

  [Refereed]


• Molecular mechanism of the aging process and rejyvenation

  SHIOMI Naofumi

  Editor, all, InTech, Aug. 2016, English, ISBN: 9789535125693

  Scholarly book


• An Assessment of the causes of lead polution an the efficiency of bioremediation by plants an microorganisms (Advances in Bioremediation of wastewater and polluted soil Chapter 11)

  Naofumi Shiomi

  Contributor, Chapter 11, Sep. 2015

  [Refereed]


• Advances in Bioremeiation of Wastewater and Polluted Soil

  Naofumi Shiomi

  Editor, All, InTech, Sep. 2015, English, ISBN: 9789535121657

  Scholarly book


• A novel bioremediation method for shallow layers of soil polluted by pesticides (Applied Bioremediarion Active and Passive Approaches Chapter 12)

  SHIOMI Naofumi

  Contributor, Chapter12, InTech, Oct. 2013, ISBN: 9789535112006

  [Refereed]


• Acid Rain Research Focus section 4

  Naofumi Shimi, Shigeo Katoh

  Contributor, Section 4, NOVA publishers, Mar. 2008, ISBN: 9781604563733

  [Refereed]


• Production of human IgM-type antibody and preparation of combinational library by recombinant Saccharomyces cerevisiae

  "Bioseparation Engineering"Vol. 16 of the book series on Progress in Biotechnology, 2000

  [Refereed]

• Polyamine-induced pluripotent stem cells and their applications

  Naofumi Shiomi

  26th INTERPEHX TOKYO, Jul. 2023, Japanese, Domestic conference

  Public discourse


• Polyamine-induced pluripotent stem cells and their application in cell medicine

  Naofumi Shiomi

  10th INTERPEHX OSAKA, Mar. 2023, Japanese

  Public discourse


• Mechanism of cold stimulus resistance and differentiation to beige adipocytes in white adipocytes

  Naofumi Shiomi, Yuki Fujiwara

  73rd SBJ Annual Meeting, Oct. 2021, Japanese

  Oral presentation


• マウス細胞の寒冷刺激耐性機構とベージュ脂肪細胞への分化

  塩見尚史, 冨士原由貴

  日本農芸化学会2021年度大会, Mar. 2021, Japanese

  Oral presentation


• Effects of explosere at low temperature on cell deffense and differentiation to beige dipocytes

  Naofumi Shiomi, Yuki Fujiwara

  Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, 2020, Mar. 2020, Japanese

  Oral presentation


• 高濃度スペルミンによる体細胞から多能性幹細胞への脱分化

  塩見 尚史

  第71回日本生物工学会大会（2019）, Sep. 2019, Japanese, Domestic conference

  Oral presentation


• Effects of low-temparature stress on the differentiation to beige adipocytes

  SHIOMI Naofumi

  70th SBJ Annual Meeting (2018) Osaka, Sep. 2018, Japanese, 早稲田大学, Domestic conference

  Oral presentation


• Effects of extract of hachinoko powder on rejuvenation of human fibroblasts

  SHIOMI Naofumi

  The Society for Biotechnology, Japan/ Annual Meeting 2017, Sep. 2017, Japanese, Domestic conference

  Oral presentation


• Characteristics of beige adipocytes induced from white adipocytes by Kikyo extract

  塩見 尚史、渡邊桂子

  68th SBJ Annual Meeting (2016), Toyama, Sep. 2016, Japanese, Domestic conference

  Poster presentation


• Effects of telmere length on the rejuvenation of cells

  SHIOMI Naofumi, Keiko Watanabe

  Annal Meeting 2015, The society for Biiotechnology, Japan, Oct. 2015, Japanese, 鹿児島 城山観光ホテル, Domestic conference

  Poster presentation


• Aging environmental impact assessment of Lead free solder created by Great Hanshin Earthquak

  Kenichiro Suetugu, SHIOMI Naofumi

  The 9th Meeting of the Institute of Life Cycle Assessment, Japan, Mar. 2015, Japanese, Domestic conference

  Oral presentation


• Characteristics of soil pollution by lead and the sorption of lead using bioremediation process

  SHIOMI Naofumi, Kenichiro Suetugu, Tomhisa Katsuda

  The 9th Meeting of the Institute of Life Cycle Assessment, Japan, Mar. 2015, Japanese, Domestic conference

  Oral presentation


• Effects of oleic acid on disfunction of macrophage

  SHIOMI Naofumi, Keiko Watanabe

  The soiety of chemical Engineers, Japan,46th Autumn Meeting, Sep. 2014, Japanese, Domestic conference

  Poster presentation


• Effects of vitamins on the differentiation to the osteoblast in a mouse C3H10T1/2 cell

  Naofumi Shiomi, Satoko Tanaka, Rumi Rakeya, Ayako Higuchi, Minami Hosokawa, Kumiko Tashiro, Keiko Watanebe

  The society for Biotechnology, Japan, 64th Annual Meeting (2014), Oct. 2012, Japanese, Domestic conference

  Oral presentation


• 骨芽細胞への分化に及ぼすビタミンの影響

  塩見尚史, 田中仁子, 武谷瑠美, 樋口絢子, 細川みなみ, 田代久美子

  化学工学会横浜大会、横浜国立大学, Aug. 2012, Japanese, Domestic conference

  Oral presentation


• Effective compounds to both unusual glucose consumption in a hepatocyte and unusual secretion of adipocytokines in an adipocyte

  Naofumi Shiomi, Yoshimi Abo, Yui Dobashi, Izumi Hirose, Mika Iwane, Yurie Seno, Mika Sumi, Mayu Yuasa

  第63回生物工学会大会、東京農工大学, Sep. 2011, Japanese, Domestic conference

  Oral presentation


• 有機リン化合物の臓器に対する毒性と分泌異常の両方に効果のある物質

  山崎由美子, 岩崎友美, 堀仁美, 緒方美穂子, 筧敦子, 塩見尚史

  第13回 化学工学学生発表会 神戸大会, Mar. 2011, Japanese, Domestic conference

  Oral presentation


• Cytotoxicity of Organnophosphorus Compounds to Liver and Pancreas Cells

  Naofumi Shiomi

  YABEC2010 16th Symposium of young Biochemical Engineer's community, Nov. 2010, English, Taipei, International conference

  Poster presentation


• Effect of fatty acid and sodium citrate on the consumption rate of glucose and productivity of choresterol in mouse hepatocyte

  Naofumi Shiomi, Miwako Maeda

  Yabec 2010, Nov. 2010, English, Domestic conference

  Poster presentation


• Effect of long-chain fatty acids on the activity of mouse macrophage

  Naofumi Shiomi, Miwako Maed

  Yabec 2010, Nov. 2010, English, International conference

  Poster presentation


• アルカンし資化酵母Yarrowia lipolyticaを用いた土壌汚染油の修復

  田沼直樹、藤本翔、勝田知尚、山地秀樹、塩見尚史

  化学工学会 第42回秋季大会 同志社大学, Sep. 2010, Japanese, Domestic conference

  Oral presentation


• 高級脂肪酸およびカルボン酸塩が肝細胞の糖代謝とコレステロール産生の及ぼす影響

  塩見尚史, 前田美和子

  化学工学会 第42回秋季大会 同志社大学, Sep. 2010, Japanese, Domestic conference

  Oral presentation


• アルカン資化酵母Yarrowia lipolyticaを用いた土壌汚染油の分解

  田沼 直樹, 藤本 翔, 勝田 知尚, 塩見 尚史, 山地 秀樹

  化学工学会第42回秋季大会, Sep. 2010

  Poster presentation


• 脂肪由来の細胞C3H10T1/2から骨芽細胞への分化を誘導する因子の探索

  塩見尚史, 渡辺歩未, 三野沙緒里, 前田美和子

  第8回 最先端バイオテクノロジー発表会, Feb. 2010

  Poster presentation


• 高級脂肪酸およびカルボン酸が肝細胞の糖代謝とコレステロール産生に及ぼす影響

  塩見尚史, 前田美和子

  化学工学会第42回秋季大会, 2010

  Poster presentation


• Glutahione biosynthesis by Yarrowia lipolitica showing high activity of glutahione synthetase

  Naofumi Shiomi, Emi Harada, Risako Nabeshima, Asako Tomoda

  The 3rd SCEJ/SSCCI Joint International Conference on Chemical Engineering, Dec. 2009

  Poster presentation


• Emi Harada, Risako Nabeshima, Naofumi Shiomi

  9th Asia Pacific Biochemical Engineering Conference, Nov. 2009

  Poster presentation


• Effect of carboxylates on triglyceride accumulation ande a TNF production in mouse cells

  Miwako Maeda, Minori Mimura, Naofumi Shiomi

  9th Asia Pacific Biochemical Engineering Conference, Nov. 2009

  Poster presentation


• Oil degradation in soil by hydrocarbon assimilationg yeast, Yarrowia lipolytica

  Mayu Fukui, Shinji Shiomi, Naoki Tamura,Naofumi Shiomi, Tomohisa Katsuda, Hideki Yamaji

  9th Asia Pacific Biochemical Engineering Conference, Nov. 2009

  Poster presentation


• 動物細胞の中性脂肪蓄積に及ぼす高級脂肪酸およびカルボン酸塩の影響

  塩見尚史, 前田美和子, 鍋島理咲子, 三村みのり

  日本農芸化学会 2009 大会, Mar. 2009, マリンメッセ福岡

  Poster presentation


• Effect of long chain fatty acids and carboxylates on triglyceride accumulation in animal cells

  Miwako Maeda, Naofumi Shiomi

  14th Symposium of Young Biochemical Engineer's Community (Yabec), Nov. 2008, English, 芝浦工業大学

  Poster presentation


• Bioremediation of soil and water acidified by acid rain with fungi cells

  Naofumi Shiomi, Tomohisa Katsuda, Shigeo Katoh

  YABEC2008 14th Symposium of young Biochemical Engineer's community, Nov. 2008, English, 芝浦工業大学

  Poster presentation


• 脂肪酸が脂肪細胞及び肝細胞の脂肪の蓄積に及ぼす影響

  前田美和子, 三村みのり, 鍋島理咲子, 塩見尚史

  化学工学会関西支部 姫路大会 2008, Nov. 2008, Japanese, ホテル日航姫路

  Oral presentation


• Yarrowia酵母中の酸化型グルタチオンの測定とその生産特性

  原田絵美, 塩見尚史

  化学工学会関西支部 姫路大会 2008, Nov. 2008, Japanese, ホテル日航姫路

  Oral presentation


• Bioremediation of polluted soil by use of self-immobilized microorganisms

  塩見尚史

  環境修復技術とその体系化に関するワークショップ Work Shop on Environmental Restoration Technologies and Systematization, Jun. 2008, 神戸大学百年記念館


• Yarrowia lipoliticaを用いた油脂からのグルタチオンの生産

  原田絵美, 石塚好, 角間彩美, 栗山翠, 野村亜矢, 鍋島理咲子, 塩見尚史

  第6回最先端バイオテクノロジー公開セミナー, Feb. 2008, Japanese, 神戸女学院大学

  Poster presentation


• マウス脂肪細胞における油脂蓄積とアディポサイトカインの生産特性

  鍋島理咲子, 三村みのり, 塩見尚史

  第6回最先端バイオテクノロジー公開セミナー, Feb. 2008, Japanese, 神戸女学院大学

  Poster presentation


• Bioremediation of polluted soil by use of self-immobilized microorganisms

  塩見尚史

  International Workshop on Energy and Environmental Research for gloval sustainability, Dec. 2007, English, 神戸ポートピアホテル

  Oral presentation


• Characteristic of triglyceride accumulation and production of adipocytokines in mouse adipocyte

  Naofumi Shiomi, Minori Mimura, Risako Nabeshima

  7th Asia Pacific Biochemical Engineering Conference, Nov. 2007, English, Taipei

  Oral presentation


• マウス脂肪細胞の油脂蓄積及びアディポサイトカイン産生特性

  三村みのり, 鍋島理咲子, 塩見尚史

  第59回生物工学会大会, Sep. 2007, Japanese

  Oral presentation


• マウス肝細胞による油脂蓄積とグルコース代謝特性

  鍋島理咲子, 三村みのり, 塩見尚史

  第59回 日生生物工学会大会, Sep. 2007, Japanese, 広島大学

  Oral presentation


• Yarrowia lipoliticaを用いた油脂からのグルタチオンの生産

  原田絵美, 石塚好, 鍋島理咲子, 塩見尚史

  第59回生物工学会大会, Sep. 2007, Japanese, 広島大学

  Oral presentation


• マウス脂肪細胞のアディポサイトカインの測定と生産特性の解析

  三村みのり, 鍋島理咲子, 塩見尚史

  化学工学会第39回秋季大会, Sep. 2007, Japanese, 北海度大学

  Poster presentation


• Yarrowia lipoliticaを用いたグルタチオンの生産

  原田絵美, 石塚好, 角間彩美, 栗山翠, 亜矢亜矢, 鍋島理咲子, 塩見尚史

  第10回化学工学会学生発表会（大阪大会）, Aug. 2007, Japanese, 関西大学

  Poster presentation


• メタノール資化性酵母を用いた一本鎖抗体生産におけるメタノール供給方法

  大西由夏, 山脇伸哉, 松本偉大, 勝田知尚, 加藤滋雄, 塩見尚史

  化学工学会 第72回年会, Mar. 2007, Japanese, 京都大学

  Oral presentation


• Characterization of triazine herbicides degradation cells and application to bioremediation

  Maiko Urase, Risako Nabeshima, Hideki Nakai, Tomohisa Katsuda, Shigeo Katoh, Naofumi Shiomi

  YABEC2006 12th Symposium of young Biochemical Engineer's community, Nov. 2006, English, Kaohsiung (Taiwan)

  Poster presentation


• Neutralization of acid in pond water by fungi

  Risako Nabeshima, Maiko Urase, Shigeo Katoh, Naofumi Shiomi

  YABEC2006 12th Symposium of young Biochemical Engineer's community, Nov. 2006, English, Kaohsiung (Taiwan)

  Poster presentation


• Measurement of adipovytokines secreted from mouse adipocyte

  Minori Mimura, Risako Nabeshima, Naofumi Shiomi

  YABEC2006 12th Symposium of young Biochemical Engineer's community, Nov. 2006, English, Kaoshiung (Taiwan)

  Poster presentation


• カビ及び藻を用いた酸性化した湖のバイオレメディエーション

  鍋島理咲子, 塩見尚史, 加藤滋雄

  化学工学会第38回秋季大会, Sep. 2006, Japanese, 福岡

  Oral presentation


• Pseudomoans sp. を用いる自己固定化バイオレメディエーションによるシアヌル酸分解

  塩見 尚史, 有井 健治, 中井 裕章, 山口 豊, 勝田 知尚, 加藤 滋雄

  化学工学会第38回秋季大会, Sep. 2006, Japanese, 福岡大学

  Oral presentation


• メタノール資化性酵母Pichia pastorisの連続培養による一本鎖抗体生産

  山脇伸哉, 松本偉大, 大西由夏, 勝田知尚, 加藤茂雄, 塩見尚史

  第58回 日本生物工学会大会, Sep. 2006, Japanese, 大阪大学

  Oral presentation


• Characterization of Cyanuric acid Degradation Cells and Application to Bioremediation

  Inha University-Kobe college ceminar, 2006

  Poster presentation


• High Sensitive Measurement of Adipocytokines Secreted from Mouse Preadipocyte 3T3-L1

  Inha University-Kobe college ceminar, 2006

  Poster presentation


• Neutralization of Acid in Pond Water with immobilized Rhizopus delmer cells

  Inha University-Kobe college ceminar, 2006

  Poster presentation


• 凝集性微生物を用いた農薬汚染土壌の浄化法の検討

  中井裕章, 山口豊, 安田多賀子, 有井健治, 坂井豊英, 勝田知尚, 加藤茂雄, 塩見尚史

  平成17年度 日本生物工学会大会, Nov. 2005


• 酸性雨により酸性化された池や土壌のバイオレメディエーション

  塩見尚史, 鍋島理咲子, 安田多賀子, 岩崎沙織, 山口豊, 中井裕章, 勝田知尚, 加藤滋雄

  関西地区3学共同合同大会, Nov. 2005


• 女子大学生の情動知能と就職活動の関連

  島井哲志、塩見尚史

  第46回日本社会心理学会, Sep. 2005

  Poster presentation


• Bioremediation of soil and ponds acidified by acid rain wit fungi cells

  Naofumi Shiomi, Noriko Kusumoto, Risako Nabeshima, Takako Yasuda, Saori Iwasaki, Yutaka Yamaguchi, Shigeo Katoh

  Asia-Pacific Engineering Conference 2005, May 2005

  Poster presentation


• 微生物によるトリアジン系化合物の分解とメラミン分解菌の特性

  第4回最先端バイオテクノロジー公開セミナー, 2005


• マウス脂肪細胞を用いた肥満に関する研究ーレプチン及びTNFの高感度測定方法と分泌特性ー

  第4回最先端バイオテクノロジー公開セミナー, 2005

  Poster presentation


• メタノール資化酵母による一本鎖抗体の効率的生産方法の検討

  平成17年度日本生物工学会大会, 2005


• 土壌より取得したメラミン分解菌の分解特性

  平成17年度日本生物工学会大会, 2005


• 中和能力を有するカビの池の酸性化防止への利用

  化学工学会第5回学生発表会, 2005


• 肝臓に有用な物質を高含有する酵母の食品への利用

  化学工学会第5回学生発表会, 2005


• 微生物の自己固定化能力を利用した農薬汚染土壌の浄化

  山口豊, 中井裕章, 藤田朋子, 塩見尚史, 加藤滋雄

  平成16年度 日本生物工学会大会, Sep. 2004


• 有用物質を蓄積する酵母の食品への利用

  塩見尚史

  第2回最先端バイオテクノロジー公開セミナー, May 2004

  Poster presentation


• 微生物の凝集性を利用した酸性雨土壌の効率的中和

  安田多賀子、山口豊、中井裕章、塩見尚史、加藤滋雄

  日本生物工学会平成15年度大会, Sep. 2003


• 異種タンパク質の連続培養生産におけるPichia pastorisの生理的応答

  李春然、仲野篤史、吉田阿礼、松本偉大、塩見尚史、加藤滋雄

  化学工学会 第36回秋季大会, Sep. 2003

  Poster presentation


• バクテリアを分解微生物とした土壌汚染浄化法の検討

  安田多賀子、岩崎沙織、山口豊、中井裕章、塩見尚史、加藤滋雄

  化学工学会 第36回秋季大会, Sep. 2003

  Poster presentation


• メチルトローフ酵母Pichia pastorisによる異種タンパク質の効率的発現

  李春然、仲野篤史、吉田阿礼、塩見尚史、加藤滋雄

  化学工学会 第68年会, Mar. 2003


• 自己固定化能力を利用した酸性雨土壌の中和

  安田多賀子, 岩崎沙織, 山口豊, 塩見尚史, 加滋滋雄

  化学工学会 第68年会, Mar. 2003


• 塩基性物質を分泌する微生物の取得と酸性雨中和への利用

  安田多賀子、岩崎沙織、山口豊、井上洋子、塩見尚史、加藤滋雄

  平成14年度 日本生物工学会大会, Oct. 2002


• 酵母のmatingにより産生される抗体の特性解析

  細川浩代, 黒田康介, 熊田陽一, 池田陽一, 塩見尚史, 加藤滋雄

  化学工学会 第35回秋季大会, Sep. 2002

  Poster presentation


• 半回分培養における組み換え酵母Pichia pastorisによる高タンパク質発現

  仲野篤史, 李春然, 吉田阿礼, 塩見尚史, 加藤滋雄

  化学工学会 第35回秋季大会, Sep. 2002

  Poster presentation


• 高効率土壌浄化法を利用した酸性雨の中和

  安田多賀子, 岩崎沙織, 勝田知尚, 塩見尚史, 加滋滋雄

  化学工学会 鳥取大会, Jul. 2002


• B.subtilisの自己固定化能力を利用した酸性雨の土壌中での中和

  安田多賀子、岩崎沙織、井上ようこ、橋本智子、勝田知尚、塩見尚史、加藤滋雄

  化学工学会 第67年会, Mar. 2002


• アデノシルメチオニンの水溶液での安定性と食品添加物としての利用

  塩見尚史、井上洋子、今村麻衣子、深川知子、安田多賀子

  平成13年度 日本生物工学会大会, Sep. 2001


• 酵母のmatingを用いた抗体ライブラリー作製法の検討

  黒田康介、古賀裕介、細川浩代、加藤茂雄、塩見尚史

  化学工学会第66年会, Apr. 2001


• 河川水に含有した油分の固定化Candida酵母による分解

  塩見尚史, 井上洋子, 安田多賀子

  化学工学会 第66年会, Apr. 2001

  Oral presentation


• Production of antibody by mating of yeast cells

  Kenji Murao, Hirohisa Koga, Motoyuki Furuta, Shigeo Katoh, Naofumi Shiomi

  化学工学会 第33回秋季大会, Sep. 2000

  Poster presentation


• バイオレメディエーションを目的としたB. subtilusの土中保持特性の検討

  井上洋子, 鶴城裕子, 安田多賀子, 塩見尚史

  日本生物工学会大会, Aug. 2000, Japanese

  Poster presentation


• 細胞の凝集性を利用した効果的な土壌及び河川の修復方法

  井上洋子, 安田多賀子, 栗原彩, 河井良恵, 塩見尚史

  化学工学会姫路大会, Jul. 2000

  Poster presentation


• 新規な冷凍耐性変異を有する酵母の冷凍パン生地への利用

  安田多賀子、鎌田恭子、前田みゆき、塩見尚史

  化学工学会 姫路大会, Jul. 2000


• Production of IgM-type antibody by secretion from yeast cells

  Kenji Murao, Hirohisa Koga, Motouki Furuta, Shigeo Katoh, Naofumi Shiomi

  化学工学会 第32回秋季大会, Sep. 1999

  Poster presentation


• Immobilization and utilization of Candida cells by use f PVF resin

  Naofumi Shiomi, Takako Yasuda, Tomoko Morita, Sayaka Inoue, Sayaka Fujii, Yuko Kondo

  化学工学会第32秋季大会, Sep. 1999, Japanese

  Poster presentation


• 組換えにSacharomyces cerevisiaeよるヒトＩｇＭ型抗体の分泌生産と抗体ライブラリー作製への利用

  塩見尚史, 村尾健司, 古賀裕久, 古田源幸, 加藤滋雄

  平成11年度 日本生物工学会大会, Sep. 1999, Japanese

  Oral presentation


• 新規な冷凍耐性酵母のスクリーニング方法

  安田多賀子, 塩見尚史, 岡田涼子, 田中久美子, 前田みゆき, 鎌田恭子, 福田泰樹, 山内宏昭

  平成11年度 日本生物工学会大会, Sep. 1999, Japanese

  Oral presentation


• Production for uman IgM antibody by recombinant Saccharomyces cerevisiae and its application to preparation of huge-scale combinatorial library

  Naofumi Shiomi, Kenji Murao, Hirohisa Koga, Shigeo Katoh

  International Conference on Bioseparation Engineering, Jul. 1999, English, Nikko, Japan

  Oral presentation


• 廃棄物の再利用と環境の修復を目的としたバイオシステムの開発

  塩見尚史

  阪神淡路大震災5周年記念事業「産業復興NEXT STAGE], 1999

  Poster presentation


• Candida 酵母の固定化と廃液処理への利用

  塩見尚史, 横関由紀

  化学工学会室蘭大会, Aug. 1998, Japanese

  Oral presentation


• 凝集性Candida酵母による乳酸廃液の連続処理

  横関由紀, 森田友子, 井上明香, 把田裕子, 塩見尚史

  日本農芸化学会1998年度大会, Mar. 1998


• Purification of recombinant proteins by anti-peptide antibodies

  Shigeo Katoh, Kenji Murao, Masaaki Terashima, Naofumi Shiomi

  4th Asia-Pacific Bioengineering Conference, Oct. 1997, English, Beijing, China

  Oral presentation


• 遺伝子組み換えPichia pastoris による水溶性ヒトCD2分子の生産と精製

  塩見,尚史, 把田,裕子, 岡本,香, 村尾健治, 加藤,滋雄

  日本生物工学会平成9年度大会, Sep. 1997, 東京農業大学世田谷キャンパス


• Purufication of CD2 by anti-peptide antibody and its characterization

  Naofumii Shiomi, Shigeo Katoh

  化学工学会第30会秋季大会, Sep. 1997, English

  Poster presentation


• High quality purification of recombinant proteins by specific elution from anti-peptide antibodies

  Shigeo Katoh, Kenji Murao, Massaki Terashima, Naofumi Shiomi

  8th European Congress on Biotecnology, Aug. 1997, Butapest, Hungary


• 食品由来の抗菌性物質の検索

  松谷恵子, 福田泰樹, 塩見尚史, 北原幹郎, 矢端端夫, 村田幸作

  日本農芸化学会1995年度大会, Mar. 1995, Japanese

  Oral presentation


• 食品由来の抗菌性物質

  松谷恵子, 福田安泰樹, 矢嶋端夫, 原史子, 塩見尚史, 木村光, 村田幸作

  日本発酵工学会平成6年度大会, Nov. 1994, 神戸国際会議場


• 食品由来の強力な新規抗菌性物質の性質

  松谷恵子, 福田泰樹, 塩見尚史, 矢島端夫, 木村光, 村田幸作

  日本農芸化学会1994年度大会, Mar. 1994, Japanese

  Oral presentation


• Saccharomyces cerevisiae のエチオニン耐性遺伝子の生化学的、遺伝学的解析

  塩見尚史, 福田秀樹, 福田泰樹, 村田幸作, 木村光

  日本発酵工学会平成2年度大会, Nov. 1990, Japanese, 大阪国際交流センター

  Oral presentation


• エチオニン耐性遺伝子を有する酵母によるS-アデノシルメチオニンの生産性の改良

  塩見尚史, 福田秀樹, 福田泰樹, 村田幸作, 木村光

  日本発酵工学会平成3年度大会, Nov. 1990, Japanese, 広島国際会議場

  Oral presentation


• 酵母のスペルミン耐性遺伝子のクローニングとS-アデノシルメシオニンの生産

  塩見尚史, 福田秀樹, 福田泰樹, 村田幸作, 木村光

  日本発酵工学会平成元年度大会, Oct. 1989, Japanese, 名古屋大学教養部

  Oral presentation


• Saccharomyces cereviasiae のエチオニン耐性遺伝子をマーカーとするプラスミドの発現と安定性

  塩見尚史, 福田秀樹, 福田泰樹, 村田幸作, 木村光

  化学工学会第56年会, Mar. 1989, Japanese

  Oral presentation


• アデノシルメチオニンの蓄積に関与する酵母の遺伝子のクローニング

  塩見 尚史, 福田 秀樹, 森川 久, 福田 泰樹, 木村 光

  日本農芸化学会（1988年度大会）, Mar. 1988, Japanese

  Oral presentation


• Enrichment and separation of metals by liquid membrane with tri-n-octylamine

  Makoto Harada, Fumio Yamazaki, Naofumi Shiomi, Motonari Adachi, Wataru Eguchi

  International Solvent Extraction Conference, Jun. 1983, English, Denver, USA

  Oral presentation


• 液体膜による二成分金属の分離

  塩見尚史, 原田誠

  化学工学会第83年会, Mar. 1983, Japanese, 関西大学

  Oral presentation


• Separation of zinc from Zn(II) and Fe(III) mixture by liquid membrane with tri-n-octylamine

  Makoto Harada, Naofumi Shiomi, Fumio Yamazaki

  Proceeding of symposium on solvent extraction, Oct. 1982, English, 静岡大学

  Oral presentation

• The institution of professsional Engineering, Japan


• THE SOCIETY OF CHEMICAL ENGINEERS, JAPAN


• JAPAN SOCIETY FOR BIOSICENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY


• THE SOCIETY FOR BIOTECHNOLOGY, JAPAN

• Food for rejuvenation: Food to prevent aging and dentima

  SHIOMI Naofumi

  Aug. 2018


• Why aging process is occurred in human being, and are we become young again?

  SHIOMI Naofumi

  Jul. 2018


• Lecture&practice ”Let's explore superfood showing the high effects on anti-obesity", Science Camp, Kobe College

  SHIOMI Naofumi

  Mar. 2017


• 実習 「微生物をもっと知ろう」 サイエンス体験、神戸女学院大学

  塩見 尚史

  Aug. 2016


• 模擬講義「ひとはなぜ老いるのか、そして若返ることはできるのか」 神戸女学院大学 オープンキャンパス

  塩見 尚史

  Aug. 2016


• Special Lecture "Why aging is occurred, and can we go back to young?" Kawanishi Midoridai High School

  SHIOMI Naofumi

  Jul. 2016


• Special lecture: "Prevention of fat by rational eating program”, Kawanishimioriai High schol

  SHIOMI Naofumi

  Jul. 2015


• Special lecture for high school stuents, "Mechanism of Obesity and Aging", Kobe College

  SHIOMI Naofumi

  Nov. 2011


• Science for elementary school stuents, " Let's obseve microorganisms"

  SHIOMI Naofumi

  Aug. 2011


• 講演「肥満とダイエットは体にどのような影響を与えているのか ー体のなかで起こっていることまで理解しよう」 まちの寺子屋市師範塾

  2010


• 模擬講義「微生物をもっと知ろう」 神戸女学院大学

  2009


• 模擬講義「食べるダイエットと食べないダイエット」 神戸女学院大学

  2009


• 「女性とダイエットー楽しみながらメタボ予防」 女性学インスチチュート 『ニュースレター』４５号

  2008


• 出張講義「バイオメディエーション法とその開発」 久留米高専

  2007


• 出張講義：「地球環境やメタボリックシンドロームについてバイオを使って研究しよう」舞子高校

  2007


• 出張講義：「身近なバイオの研究をしよう-理科からサイエンスへ」百合学院高校

  2007


• 「生活環境の保全に対する細胞工学からのアプローチ」神戸女学院大学研究所発表会

  2007


• 講演：「湖と土壌の効果的なバイオレメディエーション法の開発」セミナー「バイオエンジニアリング最前線」神戸大学工学部創造工学スタジオ

  2006


• 模擬授業：「あなたの知らない微生物の世界―実習を通して微生物の世界を体感しよう」神戸女学院大学

  2006


• 模擬授業：「理学館探検！バイオサイエンスの実験室を覗いてみよう」 神戸女学院大学

  2006


• 実験講座：｢微生物をもっと知ろう｣ バイオサイエンスセミナー「聴いて判ろう、やって解ろう！バイオの疑問」日本生物工学会関西支部主催 関西大学 工学部 生物工学実験場

  2006


• 「多様な認識特性の抗体の創出、相互作用、検出および生産のための統合システム」日本学術振興会基盤研究C2 研究分担者

  2004 - 2005


• 「メタノチオネインポリマーゲルを利用したガリウムの回収システムの開発」日本学術振興会研究意基盤研究C2 研究分担者

  2004 - 2005


• 実験講座：「微生物をもっと知ろう」星陵高校 （神戸女学院大学）

  2005


• 出張講義：「バイオサイエンスを体験してみよう」雲雀丘高校1day college

  2005


• 実験講座：「微生物をもっと知ろう」 日本生物工学会 関西支部主催「バイオってなんや？０5(関西大学）

  2005


• 実験講座：「微生物をもっと知ろう」 日本生物工学会 関西支部主催「バイオってなんや？０4(関西大学）

  2004


• 「組み換えタンパク質分泌生産効率化のための迅速プロテオーム解析システム」日本学術振興会科学研究費基盤研究(b)(1) 研究分担者

  2002 - 2003


• 実習講座：「応用生命科学 体験コース」 第4回研究室体験実習（神戸女学院大学）

  2003


• 「微生物をもっと知ろう」 日本生物工学会 関西支部主催「バイオってなんやろ？実験で学ぶバイオ０３(関西大学）

  2003


• 模擬講義 「人間環境科学」千里高校

  2003


• 「生物工学から見た人間環境科学ー神戸女学院大学での教育の進め方」神戸女学院大学専門部会主催講演会

  2003


• 「酵母のmatingを利用する大規模ライブラリーの作製と迅速スクリーニング」日本学術振興会科学研究費基盤研究（C)(２) 研究分担者

  2001 - 2002


• 「前発酵後も冷凍耐性を維持できる酵母の育種とその利用」エリザベス・アーノルド富士財団報告書

  2001 - 2002


• 「食品環境と健康についてー考えながら食べると健康になれる」神戸女学院大学女性インスティチュート主催（宝塚市立女性センター・エル）

  2001


• 「食品の安全性について」神戸女学院大学 女性学インスティチュート news letter

  1999


• 「地球を救うバイオテクノロジー」神戸女学院大学研究所主催講演会

  1998

• ブライト脂肪細胞への効果的な分化に関する研究

  塩見尚史

  神戸女学院 人間科学部, 人間科学部 教育研究助成金, 神戸女学院, Apr. 2022 - Mar. 2023, Principal investigator

  Others


• 最適なブライト脂肪細胞への分化方法の探索

  塩見尚史

  神戸女学院 人間科学部, 人間科学部 教育研究助成金, 神戸女学院, Apr. 2021 - Mar. 2022, Principal investigator

  Others


• 低温刺激に対する耐性のメカニズムとブライト脂肪細胞への分化

  塩見尚史

  神戸女学院 研究所, 人間科学部 教育研究助成金, 神戸女学院, Apr. 2020 - Mar. 2021, Principal investigator


• 白色脂肪細胞からベージュ脂肪細胞への効率的な分化誘導

  塩見尚史

  神戸女学院 研究所, 研究助成金, 神戸女学院, Apr. 2019 - Mar. 2020, Principal investigator

  Others


• ストレスによる生体応答の評価に関する領域融合的な研究

  塩見尚史

  神戸女学院 研究所, 総合研究助成金, 神戸女学院, Apr. 2019 - Mar. 2020, Coinvestigator

  Others


• 細胞の若返りに関する研究2

  神戸女学院 人間科学部, 人間科学部 教育研究助成金, 神戸女学院, Apr. 2019 - Mar. 2020, Principal investigator

  Others


• 環境ストレスが細胞や個体に及ぼす影響に関する総合的研究2

  塩見尚史

  神戸女学院 研究所, 総合研究助成金, 助成金, 神戸女学院, Apr. 2018 - Mar. 2019, Principal investigator

  Others


• 低温刺激が褐色およびブライト脂肪細胞への分化に及ぼす影響

  塩見尚史

  神戸女学院 研究所, 研究助成金, 助成金, 神戸女学院, Apr. 2018 - Mar. 2019, Principal investigator

  Others


• 細胞の若返りに関する研究

  塩見尚史

  神戸女学院 人間科学部, 人間科学部 教育研究助成金, 助成金, 神戸女学院, Apr. 2018 - Mar. 2019, Principal investigator

  Others


• 環境ストレスが細胞や個体に及ぼす影響に関する研究

  塩見尚史

  神戸女学院 研究所, 総合研究助成金, 助成金, 神戸女学院, Apr. 2017 - Mar. 2018, Principal investigator

  Others


• Investigation of effective ingredient enhancing telomerase activity and its efects on anti-aging

  SHIOMI Naofumi

  Reserch Institude, Kobe College, Apr. 2017 - Mar. 2018, Principal investigator

  Others


• 肥満と2型糖尿病の予防に関する研究2－糖代謝異常を改善する方法の探索－

  塩見 尚史

  神戸女学院大学, 人間科学部教育研究助成金, Apr. 2017 - Mar. 2018, Principal investigator

  Others


• 肥満と2型糖尿病の予防に関する研究 ー糖代謝異常と脂肪代謝異常を改善する化合物の探索ー

  塩見尚史

  神戸女学院 人間科学部, 人間科学部 教育研究助成金, 助成金, 神戸女学院, Apr. 2016 - Mar. 2017, Principal investigator

  Others


• 桔梗抽出液による脂肪細胞からベージュ脂肪細 胞の誘導

  塩見尚史

  神戸女学院 研究所, 研究助成金, 助成金, Apr. 2016 - Mar. 2017, Principal investigator

  Others


• 環境ストレスと加齢 ー発生から老化にいたる生体のストレス応答ー

  塩見尚史

  神戸女学院 研究所, 総合研究助成金, 助成金, 神戸女学院, Apr. 2016 - Mar. 2017, Coinvestigator

  Others


• 細胞の老化を抑制する化合物の探索及び老化防止のメカニズムの解明

  塩見 尚史

  神戸女学院大学, 人間科学部教育・研究助成金, 助成金, Apr. 2015 - Mar. 2016, Principal investigator

  Others


• 環境ストレスの生物作用に関する集学的研究ー蛍光色素を用いた細胞、組織レベルでのストレス応答の解析ー

  神戸女学院 研究所, 総合研究助成金, 助成金, 神戸女学院, Apr. 2014 - Mar. 2015, Coinvestigator

  Others


• Study on preventin of diabeates -1

  SHIOMI Naofumi

  Kobe College, Grant-in-aid for Education and Research at Human Science, Apr. 2014 - Mar. 2015, Principal investigator

  Others


• 微生物を利用した農薬及び化学物質のバイオレメディエーションに関する研究2

  塩見尚史

  神戸女学院 人間科学部, 人間科学部 教育研究助成金, 助成金, 神戸女学院, Apr. 2013 - Mar. 2014, Principal investigator

  Others


• ガンやアレルギー予防のための食生活に関する研究

  塩見尚史

  神戸女学院 研究所, 研究助成金, 助成金, 神戸女学院, Apr. 2013 - Mar. 2014, Principal investigator

  Others


• 骨粗鬆症の予防に関する研究-幹細胞から骨芽細胞への分化に及ぼすビタミンの影響

  塩見尚史

  神戸女学院 研究所, 研究助成金, 助成金, 神戸女学院, Apr. 2012 - Mar. 2013, Principal investigator

  Others


• 微生物を利用した農薬および化学物質のバイオレメディエーションに関する研究ー1

  塩見尚史

  神戸女学院 人間科学部, 人間科学部 教育研究助成金, 助成金, 神戸女学院, Apr. 2012 - Mar. 2013

  Others


• 抗酸化物質の探索と疾病治療に向けた集学的研究

  代表者, 西田昌司, 共同研究者, 塩見 尚史

  神戸女学院大学研究所, 総合研究助成金, Apr. 2012 - Mar. 2013, Principal investigator

  Competitive research funding


• Comprehensive study on the efficient use of resources and remeiation of environment

  塩見 尚史

  神戸女学院大学研究所, 総合助成金, 助成金, Apr. 2011 - Mar. 2012, Principal investigator

  Others


• 細胞のアンチエイジングと再生に関する研究－1

  塩見 尚史

  神戸女学院大学人間科学部, 教育研究助成金, 助成金, Apr. 2011 - Mar. 2012, Principal investigator

  Others


• Decrease of toxicity of fatty acis and prevention of metabolic synrme

  Naofumi Shiomi

  Laboratories at Kobe College, Grant-in-aid for Research, Apr. 2011 - Mar. 2012, Principal investigator

  Others


• Characteristics of the differentiation in mesenchymal stem cells and their utilization to regenerative medicine

  Naofumi Shiomi

  School of Human Sciences, Kobe COllege, Grant-in-aid for Eucation and Research, 神戸女学院, Apr. 2010 - Mar. 2011, Principal investigator

  Others


• Effects of cross talk between cells on metabolic syndrome

  Naofumi Shiomi

  Kobe College, Grant-in-aid for Reserch, Apr. 2010 - Mar. 2011, Principal investigator

  Others


• メタボリックシンドロームの予防に効果的な物質の探索

  塩見尚史

  神戸女学院 人間科学部, 人間科学部 教育研究助成金, 神戸女学院, Apr. 2009 - Mar. 2010, Principal investigator


• Yarrowia lipolyticaを用いた廃油脂の分解と有効利用

  神戸女学院 研究所, 研究助成金, 神戸女学院, Apr. 2009 - Mar. 2010, Principal investigator

  Others


• 脂肪細胞を用いたメタボリックシンドロームの研究ーTNFαの高感度測定法の開発と分解特性の開発

  神戸女学院 研究所, 研究助成金, 神戸女学院, Apr. 2007 - Mar. 2008, Principal investigator

  Others


• 脂肪の蓄積に伴う細胞郡の挙動に関する研究

  神戸女学院 人間科学部, 人間科学部 教育研究助成金, 神戸女学院, Apr. 2007 - Mar. 2008

  Others


• 海水や土壌の油汚染に対する効果的なバイオレメディエーション法の開発

  神戸女学院 研究所, 研究助成金, 助成金, 神戸女学院, Apr. 2006 - Mar. 2007, Principal investigator

  Others


• 湖のバイオレメディエーション法の開発

  神戸女学院 研究所, 研究助成金, 助成金, 神戸女学院, Apr. 2006 - Mar. 2007, Principal investigator

  Others


• 環境ホルモン関連物質分解菌の特性とその利用

  塩見尚史

  神戸女学院 研究所, 研究助成金, 助成金, 神戸女学院, Apr. 2005 - Mar. 2006, Principal investigator

  Others


• 環境物質により汚染された土壌及び地下水の微生物による修復

  塩見尚史

  神戸女学院 人間科学部, 人間科学部 教育研究助成金, 神戸女学院, Apr. 2005 - Mar. 2006, Principal investigator

  Others


• 固定化農薬分解菌による地下水中の農薬の除去

  塩見尚史

  神戸女学院 研究所, 研究助成金, 助成金, 神戸女学院, Apr. 2004 - Mar. 2005, Principal investigator

  Others


• BSISの実用化と応用に向けた検討

  神戸女学院 人間科学部, 人間科学部 教育研究助成金, 助成金, 神戸女学院, Apr. 2004 - Mar. 2005, Principal investigator

  Others


• 神戸女学院大学におけるキャリア教育に関するに関する調査研究

  塩見尚史

  神戸女学院 研究所, 総合研究助成金, 助成金, 神戸女学院, Apr. 2004 - Mar. 2005, Principal investigator

  Others


• Development of a gallium ion recovery system using metallothionein polymer gel

  TERASHIMA Masaki, SHIOMI Naofumi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe College, 2004 - 2005

  We have found that metallothionein, a protein known to adsorb cadmium ions, also adsorbs a rare metal gallium ion. In this research, we have tried to create metallothionein polymer genes using a mixture of oligonucleotides. Our final goal is to develop a gallium recovery system from waste electronic devices and factories manufacturing electronics with use of metallothionein polymer gels. First, we have expressed metallothionein dimmer proteins in E.coli The purified dimmer proteins were immobilized on the gel. We have found the following results from the adsorption experiments using the metallothionein immobilized gel and the metallothionein dimmer immobilized gel. (1)Metallothionein dimmer adsorbed gallium ions two times as much as the monomer protein. (2)The dimmer protein shows higher adsorption capacity than the monomer when gallium ion concentration was lower than 0.2 mM. (3)Adsorption isotherm of the dimmer was explained by Langmuir-type equation. To improve the efficiency of the adsorption, we have developed other gene constructs using different promoter system and a peptide-tag for purification. During this modification, a tetramer metallothionein gene has been obtained. The sequence of the tetramer gene had been confirmed by DNA sequencing. We have found that tetramer protein was accumulated as inclusion body in E.coli. Size of the tetramer protein was confirmed by Western blot analysis. To develop the effective recover process, we should develop the effective expression system in E.coli, and the proper refolding process.


• 健康生活系図書の補充

  神戸女学院 人間科学部, 人間科学部 教育研究助成金, 神戸女学院, Apr. 2003 - Mar. 2004, Principal investigator


• 固定化微生物を用いた農薬や酸性雨による土壌汚染の効率的修復

  塩見尚史

  神戸女学院 研究所, 研究助成金, 助成金, 神戸女学院, Apr. 2003 - Mar. 2004, Principal investigator

  Others


• Integrate System for Creation, Detection and Production of Antibodies with Variety of Characteristics

  KATOH Shigeo, KATUSDA Tomohisa, SHIOMI Naofumi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 2003 - 2004

  In the fields of development of pharmaceuticals, medical diagnosis-analysis and bioprocesses, production of variety of antibodies, detection of the interactions between antibody and protein, screening methods of antibodies and effective production processes of antibodies are essential. In this work, we developed the simple and rapid methods to obtain antibody ligands for recognition and detection of target proteins, based on our recent researches on production of large libraries of antibodies, a high sensitivity detection method of proteins and effective production of antibodies by secretion from yeast cells. 1.A colony lift assay for detection of antibody secreted from E. coli was developed by use of antigen-adsorbed PVDF membrane and liposome immunoblotting assay. By use of this method, high density and micro-assay was possible and the number of samples treated was increased. 2.By use of HRP encapsulating immunoliposomes and hydrophobic membranes, an immunoblotting assay method with two orders of magnitude higher sensitivity, compared with the conventional immunoblotting assay using HRP-conjugated antibody, was developed. 3.A large library of whole antibodies was obtained based on mating of yeast cells transformed by libraries of H- and L-chains, and aproduction method of antibodies by secretion from yeast cells was established. 4.An affinity biopanning method utilizing immunoliposomes was developed and it was applied to biopanning selection with a high efficiency.


• 微生物による酸性土壌の修復

  塩見尚史

  神戸女学院 研究所, 研究助成金, 助成金, 神戸女学院, Apr. 2002 - Mar. 2003, Principal investigator

  Others


• 動物細胞の油脂蓄積特性とメタボリックシンドロームの予防

  バイオテクノロジー先端技術開発研究, 2003

  細胞の脂肪蓄積とアディポネクチンの分泌挙動を細胞工学的に解析することにより、肥満予防や肥満に伴う合併症の予防を考える。

  Competitive research funding


• Development of rapid proteomic analysis for effective production of recombinant proteins by secretion

  KATOH Shigeo, TANAKA Hisao, SHIOMI Naofumi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 2001 - 2002

  Recombinant proteins have been produced by secretion from recombinant cells, since biologically active proteins are obtained through easy separation processes. However, the productivity of target proteins is not always high because of incorrect processing of signal sequences and degradation by proteases. In this work we propose an effective control and production method for secretion of recombinant proteins based on the proteomic analysis with rapid characterization of produced proteins and measurements of the activities of marker enzymes and proteases. As a model protein α-amylase was secreted from S. cerevisiae and Pichia pastoris, and the effects of fermentation conditions on the productivity, the activities of marker enzymes and the characteristics of secreted protein were studied. The following results were obtained; * Characteristics of C-terminus of secreted α-amylase were clarified by use of an anti-peptide antibody against the C-terminal sequences. * Pichia pastoris could effectively secrete α-amylase by induction with methanol, and the feed of methanol could be controlled by the DO-statt for effective production. * A novel membrane blotting method utilizing immunoliposomes was developed for rapid and highly sensitive measurement of proteins. * The activities of enzymes in the metabolic pathway of methanol increased by induction with methanol and were related to the specific production rate of α-amylase.


• Development of production method of large size of combinatorial libraries by mating of Sccharomyces cerevisiae cells and screening

  KATOH Shigeo, SHIOMI Naofumi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), KOBE UNIVERSITY, 2000 - 2001

  Recent years a variety of bioactive materials are widely used in many biological fields. To make sure obtaining bioactive materials with desired characteristics by screening, it is necessary to make huge libraries of these biomaterials. Numbers of species in a library, however, are limited depending on construction methods of libraries. In this work we try to devise a new method for production of a larger size of combinatorial libraries by use of mating characteristics of yeast cells. There are two kinds of mating types in S. cerevisiae, a-type and α-type, and they can make a diploid, which carries both the genes originated from a-type and α-type. For example, by transforming libraries of genes of heavy and light chains to a- and α-types respectively, it should be possible to obtain a larger size of libraries of antibodies by mating them. Further, since antibodies produced can be designed to be secreted from transformants, straines for production of antibodies may be easily screened. To confirm our method, the expression vectors containing heavy chain gene (μ) and light chain genes (κ) of human antibody against exotoxin were transformed into a-type and α-type strains of S. cerevisiae, respectively and these transformants were mated by mixing the cultured cells of both types. Secretion of completely assembled antibody was confirmed. It was shown that a library can be doubled by this method. Further, the effects of media, cell concentration and the ratio of a-type cell to α-type cell on the mating efficiency were studied.


• 前発酵後も冷凍耐性を維持できる酵母の育種とその利用

  エリザベスアーノルド富士財団, 学術研究助成, 公益財団法人エリザベス・アーノルド富士財団, May 1998 - May 2000, Principal investigator


• 遺伝子組換え酵母による有用物質の効率的な生産

  バイオテクノロジー先端技術開発研究, 1986

  遺伝子組み換え酵母を用いて、抗体、アデノシルメチオニンなどの有用物質の生産方法について検討した。

  Competitive research funding

• patent

  Naofumi Shiomi

  特願2018-557524(P2018-557424), 21 Jul. 2017, Kobe College, 2018, WO2018/116511, 28 Jun. 2018, 特許7033318, 02 Mar. 2022, 10 Mar. 2022, Kobe University, Pct, Domestic

  Patent right


• 若返り細胞の製造方法

  塩見尚史

  特願018-98142(P2018-98142) (22), 22 May 2018, 学校法人神戸女学院, 特開2019-201576(P2019-201576A) (43), 28 Nov. 2019

  Patent right


• 共重合体の合成遺伝子による形質転換体および共重合体の製造方法

  塩見尚史

  特願6-84084 (22), 29 Mar. 1994, 鐘淵化学工業株式会社, 特開平7-265065 (43), 17 Oct. 1995, 鐘淵化学工業株式会社, 特開平07-265065

  Patent right


• アエロモナス属の菌株の形質転換方法および得られる形質転換体

  塩見尚史

  特願平5-354356 (22), 28 Dec. 1993, 鐘淵化学工業株式会社, 特開平7-194379 (43), 01 Aug. 1995, 特開平05-3554356

  Patent right


• 補酵素の回収方法

  塩見尚史, 福田秀樹

  特願昭61-150483, 25 Jun. 1986, 鐘ヶ淵化学工業株式会社, 特開昭63-7788, 13 Jan. 1988, 特許1863903, 08 Aug. 1994, 1994/08/08, 特開昭63-7788

  Patent right


• プラスミド

  塩見尚史, 福田秀樹, 木村光, 村田幸作

  特願平3-90782 (22), 22 Apr. 1991, 鐘淵化学工業株式会社, 特開平4-320690 (43), 11 Nov. 1992, 鐘淵化学工業株式会社

  Patent right


• Ｓ－アデノシル-L-ホモシステインの製造方法

  塩見尚史, 福田秀樹, 木村光, 村田幸作

  特願平1-271040 (22), 17 Oct. 1989, 鐘淵化学工業株式会社, 特開平3-133388 (43), 06 Jun. 1991, 鐘淵化学工業株式会社, 特開平03-133388

  Patent right


• S-アデノシル-L-メチオニンの蓄積を高める遺伝子及びその用途

  塩見尚史

  特願昭62-333754 (22), 28 Dec. 1987, 鐘淵化学工業株式会社, 特開平1-174386 (43), 10 Jul. 1989, 鐘淵化学工業株式会社, 特開昭62-333754

  Patent right


• 真球状重合体粒子の製造方法

  塩見尚史

  特願昭59-222468 (22), 22 Oct. 1984, 鐘淵化学工業株式会社, 特開昭61-101504 (43), 20 May 1986, 特開昭60-69110

  Patent right