Research activity information

• Mar. 2013 第7回ゲノム微生物学会, 優秀発表賞（ポスター発表・博士課程の部）, 液体ハンドリングのみによる微生物ゲノムの連続改変

  TOMINAGA MASAHIRO

  Japan society

• Production of borneol, camphor, and bornyl acetate using engineered Saccharomyces cerevisiae.

  Masahiro Tominaga, Kazuma Kawakami, Hiro Ogawa, Tomomi Nakamura, Akihiko Kondo, Jun Ishii

  Microbial production of bicyclic monoterpenes is of great interest because their production primarily utilizes non-sustainable resources. Here, we report an engineered Saccharomyces cerevisiae yeast that produces bicyclic monoterpenes, including borneol, camphor, and bornyl acetate. The engineered yeast expresses a bornyl pyrophosphatase synthase from Salvia officinalis fused with mutated farnesyl pyrophosphate synthase from S. cerevisiae and two mevalonate pathway enzymes (an acetoacetyl-CoA thiolase/hydroxymethylglutaryl-CoA [HMG-CoA] reductase and an HMG-CoA synthase) from Enterococcus faecalis. The yeast produced up to 23.0 mg/L of borneol in shake-flask fermentation. By additionally expressing borneol dehydrogenase from Pseudomonas sp. TCU-HL1 or bornyl acetyltransferase from Wurfbainia villosa, the engineered yeast produced 23.5 mg/L of camphor and 21.1 mg/L of bornyl acetate, respectively. This is the first report of heterologous production of camphor and bornyl acetate.

  Jun. 2025, Metabolic engineering communications, 20, e00259, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Designing strong inducible synthetic promoters in yeasts

  Masahiro Tominaga, Yoko Shima, Kenta Nozaki, Yoichiro Ito, Masataka Someda, Yuji Shoya, Noritaka Hashii, Chihiro Obata, Miho Matsumoto-Kitano, Kohei Suematsu, Tadashi Matsukawa, Keita Hosoya, Noriko Hashiba, Akihiko Kondo, Jun Ishii

  Lead, Dec. 2024, Nature Communications

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Screening of protein-based inhibitors for the intracellular domain of epidermal growth factor receptor by directed evolution using the yeast Gγ recruitment system

  Ririka Asama, Masahiro Tominaga, Sayaka Ito, Yoichiro Ito, Kazuhiro Takemura, Shun Sakuraba, Kohei Katsurada, Nobuo Fukuda, Akihiko Kondo, Jun Ishii

  Nov. 2024, Journal of Bioscience and Bioengineering

  [Refereed]

  Scientific journal


• Simple workflow for high-titer microbial production of small antibodies

  Jun Ishii, Masahiro Tominaga, Yasuyuki Nakamura, Daisuke Sasaki, Kazumasa Ogura, Kohei Suematsu, Yoichiro Ito, Akihiko Kondo

  Increasing demand for biologics necessitates the rapid production of antibodies and their mimetics. Herein, we established a rapid workflow to develop a fermentation process for high-titer production of small antibodies using the yeast Komagataella phaffii. We screened yeast strains with the optimal gene copy number of target proteins and applied them to a feedback-controlled, high-cell density fed-batch fermentation, and achieved high-titer production of small antibodies with titers 3–7 g/L.

  May 2024


• Droplet-based microfluidic platform for detecting agonistic peptides that are self-secreted by yeast expressing a G-protein-coupled receptor.

  Ririka Asama, Cher J S Liu, Masahiro Tominaga, Yu-Ru Cheng, Yasuyuki Nakamura, Akihiko Kondo, Hsiang-Yu Wang, Jun Ishii

  BACKGROUND: Single-cell droplet microfluidics is an important platform for high-throughput analyses and screening because it provides an independent and compartmentalized microenvironment for reaction or cultivation by coencapsulating individual cells with various molecules in monodisperse microdroplets. In combination with microbial biosensors, this technology becomes a potent tool for the screening of mutant strains. In this study, we demonstrated that a genetically engineered yeast strain that can fluorescently sense agonist ligands via the heterologous expression of a human G-protein-coupled receptor (GPCR) and concurrently secrete candidate peptides is highly compatible with single-cell droplet microfluidic technology for the high-throughput screening of new agonistically active peptides. RESULTS: The water-in-oil microdroplets were generated using a flow-focusing microfluidic chip to encapsulate engineered yeast cells coexpressing a human GPCR [i.e., angiotensin II receptor type 1 (AGTR1)] and a secretory agonistic peptide [i.e., angiotensin II (Ang II)]. The single yeast cells cultured in the droplets were then observed under a microscope and analyzed using image processing incorporating machine learning techniques. The AGTR1-mediated signal transduction elicited by the self-secreted Ang II peptide was successfully detected via the expression of a fluorescent reporter in single-cell yeast droplet cultures. The system could also distinguish Ang II analog peptides with different agonistic activities. Notably, we further demonstrated that the microenvironment of the single-cell droplet culture enabled the detection of rarely existing positive (Ang II-secreting) yeast cells in the model mixed cell library, whereas the conventional batch-culture environment using a shake flask failed to do so. Thus, our approach provided compartmentalized microculture environments, which can prevent the diffusion, dilution, and cross-contamination of peptides secreted from individual single yeast cells for the easy identification of GPCR agonists. CONCLUSIONS: We established a droplet-based microfluidic platform that integrated an engineered yeast biosensor strain that concurrently expressed GPCR and self-secreted the agonistic peptides. This offers individually isolated microenvironments that allow the culture of single yeast cells secreting these peptides and gaging their signaling activities, for the high-throughput screening of agonistic peptides. Our platform base on yeast GPCR biosensors and droplet microfluidics will be widely applicable to metabolic engineering, environmental engineering, and drug discovery.

  Apr. 2024, Microbial cell factories, 23(1) (1), 104 - 104, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Plant Flavonoid Production in Bacteria and Yeasts

  Shota Isogai, Masahiro Tominaga, Akihiko Kondo, Jun Ishii

  Flavonoids, a major group of secondary metabolites in plants, are promising for use as pharmaceuticals and food supplements due to their health-promoting biological activities. Industrial flavonoid production primarily depends on isolation from plants or organic synthesis, but neither is a cost-effective or sustainable process. In contrast, recombinant microorganisms have significant potential for the cost-effective, sustainable, environmentally friendly, and selective industrial production of flavonoids, making this an attractive alternative to plant-based production or chemical synthesis. Structurally and functionally diverse flavonoids are derived from flavanones such as naringenin, pinocembrin and eriodictyol, the major basic skeletons for flavonoids, by various modifications. The establishment of flavanone-producing microorganisms can therefore be used as a platform for producing various flavonoids. This review summarizes metabolic engineering and synthetic biology strategies for the microbial production of flavanones. In addition, we describe directed evolution strategies based on recently-developed high-throughput screening technologies for the further improvement of flavanone production. We also describe recent progress in the microbial production of structurally and functionally complicated flavonoids via the flavanone modifications. Strategies based on synthetic biology will aid more sophisticated and controlled microbial production of various flavonoids.

  Frontiers Media SA, Jul. 2022, Frontiers in Chemical Engineering, 4

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Enhanced squalene production by modulation of pathways consuming squalene and its precursor.

  Masahiro Tominaga, Keita Miyazaki, Shoko Hataya, Yasumasa Mitsui, Shuji Kuroda, Akihiko Kondo, Jun Ishii

  Fermentative production of squalene in yeast as an alternative approach to extracting squalene from sharks or plants has attracted significant interest. However, squalene accumulation is limited due to its inevitable high-flux allocation toward ergosterol synthesis. In this study, we described expression control of squalene monooxygenase (Erg1p), the first-step enzyme of ergosterol synthesis from squalene, to significantly reduce squalene loss. We replaced the ERG1 promoter (PERG1) with three natural yeast promoters with different activities (PPCL2, PHCM1, and PTHI2). ERG1 controlled by PTHI2 showed 20 times higher squalene production compared with the wild-type strain, whereas the other two strains exhibited no significant difference. By combining the overexpression of rate-limiting enzyme and the deletion of non-essential competing pathway gene, the yeast Saccharomyces cerevisiae produced up to 379 mg/L of squalene.

  Lead, Jul. 2022, Journal of bioscience and bioengineering, 134(1) (1), 1 - 6, English, Domestic magazine

  [Refereed]

  Scientific journal


• Engineering of Synthetic Transcriptional Switches in Yeast

  Masahiro Tominaga, Akihiko Kondo, Jun Ishii

  Lead, Apr. 2022, LIFE-BASEL, 12(4) (4), English

  [Refereed]

  Link to Kobe Univ. Repository (Kernel)


• Constitutive cell surface expression of ZZ domain for the easy preparation of yeast-based immunosorbents.

  Kohei Katsurada, Masahiro Tominaga, Misato Kaishima, Hiroko Kato, Toshihide Matsuno, Chiaki Ogino, Akihiko Kondo, Jun Ishii, Katsumi Takayama

  We describe a novel expression cassette that enables efficient and constitutive expression of the ZZ domain derived from Staphylococcus aureus protein A on the yeast cell surface to easily prepare yeast-based immunosorbents. Using this expression cassette containing the PGK1 promoter, a secretion signal derived from α-factor, and a Flo1-derived anchor protein, we successfully created a yeast-based immunosorbent for human serum albumin.

  Dec. 2021, The Journal of general and applied microbiology, 67(6) (6), 265 - 268, English, Domestic magazine

  [Refereed]

  Scientific journal


• Robust and flexible platform for directed evolution of yeast genetic switches

  Masahiro Tominaga, Kenta Nozaki, Daisuke Umeno, Jun Ishii, Akihiko Kondo

  Lead, Mar. 2021, NATURE COMMUNICATIONS, 12(1) (1), English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Positive Feedback Genetic Circuit Incorporating a Constitutively Active Mutant Gal3 into Yeast GAL Induction System

  Shintaro Ryo, Jun Ishii, Toshihide Matsimo, Yasuyuki Nakamura, Daiki Matsubara, Masahiro Tominaga, Akihiko Kondo

  Jun. 2017, ACS SYNTHETIC BIOLOGY, 6(6) (6), 928 - 935, English

  [Refereed]

  Scientific journal


• Rapid Diversification of Betl-Based Transcriptional Switches for the Control of Biosynthetic Pathways and Genetic Circuits

  Kazuya Saeki, Masahiro Tominaga, Shigeko Kawai-Noma, Kyoichi Saito, Daisuke Umeno

  Nov. 2016, ACS SYNTHETIC BIOLOGY, 5(11) (11), 1201 - 1210, English

  [Refereed]

  Scientific journal


• From mannan to bioethanol: cell surface co-display of beta-mannanase and beta-mannosidase on yeast Saccharomyces cerevisiae

  Jun Ishii, Fumiyoshi Okazaki, Apridah Cameliawati Djohan, Kiyotaka Y. Hara, Nanami Asai-Nakashima, Hiroshi Teramura, Ade Andriani, Masahiro Tominaga, Satoshi Wakai, Prihardi Kahar, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

  Sep. 2016, BIOTECHNOLOGY FOR BIOFUELS, 9(1) (1), 188, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Rapid and Liquid-Based Selection of Genetic Switches Using Nucleoside Kinase Fused with Aminoglycoside Phosphotransferase

  Masahiro Tominaga, Kohei Ike, Shigeko Kawai-Noma, Kyoichi Saito, Daisuke Umeno

  Lead, Mar. 2015, PLOS ONE, 10(3) (3), e0120243, English

  [Refereed]

  Scientific journal


• Liquid-Based Iterative Recombineering Method Tolerant to Counter-Selection Escapes

  Masahiro Tominaga, Shigeko Kawai-Noma, Ikuro Kawagishi, Yoshiyuki Sowa, Kyoichi Saito, Daisuke Umeno

  Lead, Mar. 2015, PLOS ONE, 10(3) (3), e0119818, English

  [Refereed]

  Scientific journal

• 酵母における遺伝子スイッチ進化工学ワークフローの「ロバスト化」—Robust Evolutionary Engineering Platform for Yeast Genetic Switches

  冨永 将大, 近藤 昭彦, 石井 純

  [京都] : 日本生物物理学会, Jun. 2024, 生物物理 / 日本生物物理学会 編, 64(3) (3), 144 - 146, Japanese


• Development of yeast transcriptional switches using bacteriophage RNA polymerase

  堀智彦, 冨永将大, 冨永将大, 梶亘佑, 近藤昭彦, 近藤昭彦, 近藤昭彦, 石井純, 石井純

  2023, 日本生物工学会大会講演要旨集, 75th


• Development of functional micro hydrogel with cell selective ability

  井元乃絵, 冨永将大, 伊藤洋一郎, 石井純, 近藤昭彦, 神谷典穂, 神谷典穂

  2023, 日本生物工学会大会講演要旨集, 75th


• バクテリオファージ由来の転写系を利用した酵母遺伝子スイッチの構築

  堀智彦, 冨永将大, 冨永将大, 梶亘佑, 近藤昭彦, 近藤昭彦, 近藤昭彦, 石井純, 石井純

  2023, 日本農芸化学会関西支部講演会講演要旨集, 528th


• mRNAワクチン：予防医薬の急先鋒？

  冨永 将大

  公益社団法人 日本生物工学会, 25 Apr. 2022, 生物工学会誌, 100(4) (4), 190 - 190, Japanese


• バクテリア転写因子CamRを用いたボルネオール生産酵母のスクリーニング系の構築

  川上和真, 冨永将大, 冨永将大, 小川ひろ, 能崎健太, 近藤昭彦, 近藤昭彦, 近藤昭彦, 近藤昭彦, 石井純, 石井純

  2022, 日本農芸化学会関西支部講演会講演要旨集, 523rd


• 酵母におけるスクアレン生合成経路の改変および下流モノオキシゲナーゼの発現調節

  宮崎敬太, 三井靖雅, 旗谷章子, 冨永将大, 近藤昭彦, 近藤昭彦, 近藤昭彦, 石井純, 石井純

  2020, 日本農芸化学会関西支部講演会講演要旨集, 513th


• 新規な3機能性融合マーカーを用いた酵母遺伝子スイッチの組織的開発

  能崎健太, 冨永将大, 梅野太輔, 近藤昭彦, 近藤昭彦, 近藤昭彦, 石井純, 石井純

  2019, 日本農芸化学会関西支部講演会講演要旨集, 511th


• 陽性/陰性選択マーカーと蛍光タンパク質から成る3機能性新規融合タンパク質を用いた酵母遺伝子スイッチの開発

  能崎健太, 冨永将大, 梅野太輔, 近藤昭彦, 近藤昭彦, 石井純, 石井純

  2019, 日本生物工学会大会講演要旨集, 71st


• 7セグメントデコーダとして振る舞う単層遺伝子回路の進化デザイン

  山上和馬, 小林一幾, 湯本達弥, 佐伯和哉, 冨永将大, 河合繁子, 斎藤恭一, 梅野太輔

  2019, 日本農芸化学会大会講演要旨集(Web), 2019


• 進化デザインによる新規テルペノイドセンサの開発

  冨永将大, 小川ひろ, 能崎健太, 近藤昭彦, 近藤昭彦, 近藤昭彦, 石井純, 石井純

  2019, 日本農芸化学会関西支部講演会講演要旨集, 511th


• ゲノム連続組み換え手法を用いたイソプレノイド生産量の高い大腸菌株の作出

  齋藤聡司, 冨永将大, LI Ling, 岩嵜美希, 河合(野間)繁子, 斎藤恭一, 梅野太輔

  2015, 日本農芸化学会大会講演要旨集(Web), 2015


• 選択カセットの再デザインと微生物ゲノムの連続編集への応用

  冨永将大, 嶋村陽, 河合(野間)繁子, 齋藤恭一, 梅野太輔

  2014, 日本ゲノム微生物学会年会要旨集, 8th


• Bet I変異体とT7RNAポリメラーゼを用いたコリン誘導系の開発

  荒澤悠輔, 池紘平, 宮口明恵, 冨永将大, 河合(野間)繁子, 斎藤恭一, 梅野太輔

  2014, 日本農芸化学会大会講演要旨集(Web), 2014


• 安定運転と弱毒化を指向したAutogeneの進化工学

  佐伯和哉, 池紘平, 宮口明恵, 冨永将大, 河合(野間)繁子, 斎藤恭一, 梅野太輔

  2014, 日本農芸化学会大会講演要旨集(Web), 2014


• 液体ハンドリングのみによって行う微生物ゲノムの連続編集

  冨永将大, 嶋村陽, 曽和義幸, 曽和義幸, 川岸郁朗, 川岸郁朗, 斎藤恭一, 梅野太輔

  2013, 日本ゲノム微生物学会年会要旨集, 7th


• 代謝経路と制御ネットワークの組織的な進化工学技術

  梅野 太輔, 冨永 将大, 古林 真衣子

  日本生物工学会, 2013, 生物工学会誌 / 日本生物工学会 編, 91(6) (6), 333 - 336, Japanese


• ヌクレオシドキナーゼを用いた微生物ゲノムの連続改変

  冨永将大, 田代洋平, 斎藤恭一, 梅野太輔

  2012, 日本農芸化学会大会講演要旨集(Web), 2012


• 2Ia14 Directed evolution of LuxR for sensitivity

  Tominaga,Masahiro, Tashiro,Yohei, Saito,Kyoichi, Umeno,Daisuke

  日本生物工学会, 25 Aug. 2011, 日本生物工学会大会講演要旨集, 63, 176, Japanese


• 3P-2141 Localization of the molecular functionalities on flagella filaments

  TOMINAGA Masahiro, SAITO Kyoichi, UMENO Daisuke

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 166 - 166, Japanese

• 代謝経路と制御ネットワークの組織的な進化工学技術

  UMENO DAISUKE, TOMINAGA MASAHIRO, MAIKO FURUBAYASHI, KOHEI IKE

  Joint work, Journal of Bioscience and Bioengineering, 2013, Japanese

  Scholarly book

• T7ファージ由来の転写系を利用した酵母遺伝子スイッチの開発

  堀智彦, 冨永将大, 梶亘佑, 近藤昭彦, 石井純

  神戸大学 研究基盤センター 若手フロンティア研究会2023, Dec. 2023


• Development of antibody binding protein-immobilized functional microhydrogels with cell sorting capability

  Noe Inomoto, Masahiro Tominaga, Yoichiro Ito, Jun Ishii, Akihiko Kondo, Noriho Kamiya

  The 34th International Symposium on Chemical Engineering (ISChE2023), Dec. 2023


• バクテリオファージ由来の転写系を利用した酵母遺伝子スイッチの構築

  堀智彦, 冨永将大, 梶亘佑, 近藤昭彦, 石井純

  日本農芸化学会関西支部第528回講演会, Dec. 2023


• 高発現性の酵母人工誘導プロモータの開発

  冨永将大, 伊藤洋一郎, 近藤昭彦, 石井純

  第3回神戸大学先端バイオ工学研究センター成果発表会, Sep. 2023


• バクテリオファージ由来転写系を利用した酵母遺伝子スイッチの開発

  堀智彦, 冨永将大, 梶亘佑, 近藤昭彦, 石井純

  第3回神戸大学先端バイオ工学研究センター成果発表会, Sep. 2023


• 細胞選別能を有する機能性微小ハイドロゲルの創製

  井元 乃絵, 冨永 将大, 伊藤 洋一郎, 石井 純, 近藤 昭彦, 神谷 典穂

  第75回日本生物工学会大会, Sep. 2023


• 酵母の高発現性人工誘導プロモータの開発

  冨永 将大, 伊藤 洋一郎, 近藤 昭彦, 石井 純

  第75回日本生物工学会大会, Sep. 2023


• バクテリオファージ由来RNAポリメラーゼを利用した酵母遺伝子スイッチの開発

  堀 智彦, 冨永 将大, 梶 亘佑, 近藤 昭彦, 石井 純

  第75回日本生物工学会大会, Sep. 2023

  Oral presentation


• メチロトローフ酵母を用いた異なる炭素源からの乳酸生産

  鈴木創太, 廣畑凌治, 中村泰之, 伊藤洋一郎, 北野美穂, 冨永将大, 中曽根光, 近藤昭彦, 石井純

  第25回化学工学会学生発表会, Mar. 2023


• バクテリア転写因子CamRを用いたボルネオール生産酵母のスクリーニング系の構築

  川上和真, 冨永将大, 小川ひろ, 能崎健太, 近藤昭彦, 石井純

  日本農芸化学会関西支部 第523回講演会, Dec. 2022


• 植物由来テルペン合成酵素を用いたボルネオール生産酵母の開発

  川上和真, 冨永将大, 小川ひろ, 能崎健太, 近藤昭彦, 石井純

  第2回神戸大学先端バイオ工学研究センター成果発表会, Oct. 2022


• 酵母におけるスクアレン生産の呈色評価系の構築

  三ツ橋拓海, 冨永将大, 宮崎敬太, 近藤昭彦, 石井純

  第2回神戸大学先端バイオ工学研究センター成果発表会, Oct. 2022


• 進化工学による酵母遺伝子スイッチの開発とテルペノイドセンサへの応用

  冨永将大, 川上和真, 小川ひろ, 能崎健太, 近藤昭彦, 石井純

  第1回神戸大学先端バイオ工学センター成果発表会, Oct. 2021


• 酵母におけるスクアレン生合成経路の改変および下流モノオキシゲナーゼの発現調節

  宮崎敬太, 三井靖雅, 旗谷章子, 冨永将大, 近藤昭彦, 石井純

  日本農芸化学会関西支部第513回講演会, Nov. 2020


• 新規な３機能性融合マーカーを用いた酵母遺伝子スイッチの組織的開発

  能崎健太, 冨永将大, 梅野太輔, 近藤昭彦, 石井純

  日本農芸化学会関西支部 支部例会（第511回講演会）, Dec. 2019


• 進化デザインによる新規テルペノイドセンサの開発

  冨永将大, 小川ひろ, 能崎健太, 近藤昭彦, 石井純

  日本農芸化学会関西支部 支部例会（第511回講演会）, Dec. 2019


• 陽性/陰性選択マーカーと蛍光タンパク質から成る3機能性新規融合タンパク質を用いた酵母遺伝子スイッチの開発

  KENTA NOZAKI, TOMINAGA MASAHIRO, DAISUKE UMENO, KONDO AKIHIKO, ISHII JUN

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学 津島キャンパス, Domestic conference

  Oral presentation


• 酵母を用いた癌標的膜タンパク質に対する相互作用阻害分子のスクリーニング系構築

  KATSURADA KOUHEI, SUGIMOTO MIHO, KAISHIMA MISATO, TOMINAGA MASAHIRO, KONDO AKIHIKO, ISHII JUN

  生物工学若手研究者の集い 夏のセミナー2019, Jul. 2019, Japanese, 琵琶湖国定公園 近江白浜 政府登録旅館 白浜荘, Domestic conference

  Poster presentation


• Directed evolution of single-layered genetic circuits that behave as 7-segment decoders

  山上和馬, 小林一幾, 湯本達弥, 佐伯和哉, 冨永将大, 河合繁子, 斎藤恭一, 梅野太輔

  日本農芸化学会2019年度大会, Mar. 2019


• Development of combinatorial gene assemble system and application to improvement of organic solvent tolerance in Escherichia coli

  Yoshinobu Saijo, Masahiro Tominaga, Kanae Sakai, Toshihide Matsuno, Jun Ishii, Akihiko Kondo

  The 9th International Symposium of Innovative BioProduction Kobe (iBioK), Feb. 2018


• 酵母遺伝子スイッチの進化工学のためのON/OFF選抜法の開発

  能崎 健太, 冨永 将大, 河合(野間) 繁子, 梅野 太輔, 石井 純, 近藤 昭彦

  生物工学若手研究者の集い 夏のセミナー2017, Jul. 2017


• 酵母代謝改変のための遺伝子のノックダウン/ノックアウト法の開発

  光増 遼太郎, 冨永 将大, 石井 純, 近藤 昭彦

  生物工学若手研究者の集い 夏のセミナー2017, Jul. 2017


• Development of Selection Method for Directed Evolution of Genetic Switches in Saccharomyces Cerevisiae

  Masahiro TOMINAGA, Shigeko KAWAI-NOMA, Daisuke UMENO, Jun ISHII, Akihiko KONDO

  Metabolic Engineering 11, Jun. 2016, English, International conference

  Poster presentation


• 2Ia14 Directed evolution of LuxR for sensitivity

  Tominaga,Masahiro, Tashiro,Yohei, Saito,Kyoichi, Umeno,Daisuke

  日本生物工学会大会講演要旨集, Aug. 2011, Japanese, 日本生物工学会


• 3P-2141 Localization of the molecular functionalities on flagella filaments

  TOMINAGA Masahiro, SAITO Kyoichi, UMENO Daisuke

  日本生物工学会大会講演要旨集, 2010, Japanese, 日本生物工学会

• 合成生物学ツールとしての酵母オペロン型遺伝子発現系の「再開発」

  冨永 将大

  科学研究費補助金／若手研究, Apr. 2018 - Mar. 2021, Principal investigator

  Competitive research funding

• 遺伝子スイッチ

  冨永 将大, 石井 純, 能崎 健太, 近藤 昭彦

  特願2020-202234, 04 Dec. 2020, 国立大学法人神戸大学, 特開2022-089660, 16 Jun. 2022

  Patent right

  IRI


• 高速かつ高効率なゲノム改変法

  梅野 太輔, 冨永 将大, 田代 洋平, 川岸 郁朗, 曽和 義幸, 稲葉 岳彦, 蔡 栄淑

  特願2012-113083, 17 May 2012, 国立大学法人 千葉大学, 学校法人法政大学, 特開2013-017473, 31 Jan. 2013

  Patent right

  IRI