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MINAMOTO ToshifumiGraduate School of Human Development and Environment / Department of Human Environmental ScienceProfessor
Researcher basic information
■ Research news- 26 May 2021, eDNA analysis could contribute towards more effective pest control
- 02 Sep. 2019, GIS and eDNA analysis system successfully used to discover new habitats of rare salamander could be utilized to find other endangered species
- 01 Mar. 2019, Endangered eel located using DNA from one liter of water
- 17 Nov. 2017, Using eDNA to identify the breeding habitat of endangered species
- 30 Jan. 2017, DNA analysis of seawater detects 80% of fish species in just one day
- 04 Sep. 2015, Invasion of non-native genotypes exposed by environmental DNA
- 04 Sep. 2015, Invasion of non-native genotypes exposed by environmental DNA
- Animal physiology
- Disease ecology
- Environmental DNA
- Environmental Science
- Molecular Ecology
- Chronobiology
- Life sciences / Animals: biochemistry, physiology, behavioral science
- Life sciences / Ecology and environmental science
- Environmental science/Agricultural science / Environmental policy and society
- Environmental science/Agricultural science / Environmental impact assessment
- Environmental science/Agricultural science / Environmental dynamics
Research activity information
■ Award- Sep. 2024 Ecology and Civil Engineering Society, Hirose Award
- May 2024 公益社団法人土木学会, 環境賞, 環境DNAを活用した生物モニタリングに向けた調査手法の構築と建設現場への適用
- Mar. 2023 Kobe City, Kobe SDGs Encouragement Award
- Jul. 2021 Ecological Society of Japan / Shiga Prefecture, The 21nd Biwako Prize for Ecology
- Mar. 2019 Murao Educational Foundation, The 36th Academic Award of Murao Educational FoundationPublisher
- Dec. 2016 National Institute of Science and Technology Policy, 科学技術への顕著な貢献2016（ナイスステップな研究者）Others
- Oct. 2015 Kobe University, President Award of Kobe UniversityOthers
- Sep. 2025, Landscape and Urban Planning, 261, 105377, English[Refereed]Scientific journal
- Elsevier BV, Jun. 2025, Global Ecology and Conservation, 59, e03511, EnglishScientific journal
- Last, Elsevier BV, Apr. 2025, Marine Environmental Research, 208, 107094, English[Refereed]Scientific journal
- Apr. 2025, 応用生態工学, (in press), Japaneseマクロ生物の環境DNA分析：発展の歴史と将来への展望[Refereed][Invited]Scientific journal
- Elsevier BV, Apr. 2025, Estuarine, Coastal and Shelf Science, 315, 109165, English[Refereed]Scientific journal
- Mar. 2025, Environmental DNA, 7, e70085, English[Refereed]Scientific journal
- Mar. 2025, Metabarcoding and Metagenomics, 9, e144340, English[Refereed]Scientific journal
- Dec. 2024, Parasitology Research, 123, 419, English[Refereed]Scientific journal
- Elsevier BV, Dec. 2024, Acta Tropica, 260, 107402, English[Refereed]Scientific journal
- Nov. 2024, Journal of Environmental Management, 370, 122676, English[Refereed]Scientific journal
- Last, Oct. 2024, Molecular Ecology Resources, 24(8) (8), e14011, English[Refereed]Scientific journal
- Ornithological Society of Japan, Sep. 2024, Ornithological Science, 23(2) (2), 125 - 128, English[Refereed]Scientific journal
- Sep. 2024, Conservation Genetics Resources, 16, 255 - 261, EnglishDetection of environmental DNA of finless porpoise (Neophocaena asiaeorientalis) in Osaka Bay, Japan[Refereed]Scientific journal
- Springer Science and Business Media LLC, Aug. 2024, Limnology, 25(3) (3), 247 - 254, English[Refereed]Scientific journal
- Elsevier BV, Jun. 2024, One Health, 18, 100715, English[Refereed]Scientific journal
- Abstract Japanese giant salamander (Andrias japonicus) is one of the largest amphibian species in the world and an iconic species in Japan. However, as its distribution has recently declined across the country, rapid and extensive monitoring of the distribution is urgently needed for its efficient conservation. Here, we used environmental DNA (eDNA) analysis to assess the Japanese giant salamander’s distribution in western Japan and, for that purpose, we collected 410 water samples from 12 rivers. We then developed a new eDNA assay for multi-copy nuclear DNA (nuDNA) of the giant salamander and compared the eDNA detectability of the nuDNA marker with that of a previous mitochondrial DNA (mtDNA) marker. Throughout the survey, we detected target eDNA from 162 water samples using either of the markers, which generally corresponded to the known natural distribution of the species. Additionally, the use of the nuDNA marker allowed for higher detection rate of target eDNA than the mtDNA marker. Moreover, the detection rate of target eDNA decreased substantially in water samples with higher conductivity and also partly in those with higher pH, suggesting their negative impacts on the salamander’s ecology. Our results demonstrated that eDNA analysis with multi-copy nuDNA marker is highly useful for efficient and sensitive surveillance of Japanese giant salamander’s distribution. Our study provided the methodology for efficiently monitoring the Japanese giant salamander’s distribution via eDNA analysis and facilitating conservation activities for them.Springer Science and Business Media LLC, Apr. 2024, Limnology, 25(2) (2), 189 - 198, English[Refereed]Scientific journal
- Last, Elsevier BV, Apr. 2024, Environmental Advances, 15, 100457, English[Refereed]Scientific journal
- Abstract Periodic monitoring can provide important information for the protection of endangered fish, sustainable use of fishery resources and management of alien species. Previous studies have attempted to monitor fish using non‐invasive environmental DNA (eDNA) technology, generally employing quantitative PCR to quantify the eDNA concentration. However, the throughput was limited. High‐throughput metabarcoding technology can detect the DNA of multiple species simultaneously in a single experiment but does not provide sufficient quantification. In this study, we applied a quantitative metabarcoding approach to simultaneously quantify the eDNA concentration of an entire fish assemblage in a small reservoir over two summer seasons. Traditional surveys were also conducted to investigate the individuals of fish. The eDNA concentrations were quantified using quantitative metabarcoding, and the fish species detected using this approach were highly consistent with the results of traditional fish monitoring. A significant positive relationship was observed between the eDNA concentration and fish species abundance. Seasonal changes in fish community structure were estimated using eDNA concentrations, which may reveal the activity seasons of different fish. The eDNA concentrations of different fish species peaked at different water temperatures, reflecting the differential responses of fish species to this environmental factor. Finally, by detecting outlier eDNA concentrations, the spawning activities of 13 fish species were estimated, 12 of which were roughly consistent with the current knowledge of fish spawning periods. These results indicate that quantitative eDNA metabarcoding with dozens of sampling times is useful for the simultaneous ecological monitoring of multiple fish species.Last, Wiley, Jan. 2024, Molecular Ecology Resources, 24, e13875, English[Refereed]Scientific journal
- Dec. 2023, Tropical Medicine and Health, 51, 71, EnglishEnvironmental detection of eumycetoma pathogens using multiplex real-time PCR for soil DNA in Sennar State, Sudan[Refereed]Scientific journal
- Last, Japan Society of Civil Engineers, Nov. 2023, Japanese Journal of JSCE, 79(17) (17), 23-27158, Japanese[Refereed]Scientific journal
- Last, Nov. 2023, Population Ecology, (in press), EnglishRegional-scale effects of deer-induced forest degradation on river ecosystem dynamics[Refereed]Scientific journal
- Nov. 2023, International Journal of Molecular Sciences, 24(21) (21), 16009, English[Refereed]Scientific journal
- Springer Science and Business Media LLC, Nov. 2023, Ichthyological Research, 70(4) (4), 409 - 418, English[Refereed]Scientific journal
- Last, Sep. 2023, Ecosphere, 14(9) (9), e4651, English[Refereed]Scientific journal
- Springer Science and Business Media LLC, Sep. 2023, Theoretical Ecology, 16(3) (3), 195 - 208, English[Refereed]Scientific journal
- Abstract This study proposes a practice and discussion for an interdisciplinary approach to policies for the conservation of suburban and peri-urban ecosystems. We highlight the need for evidence-based assessment of the current quality of the targeted nature from perspectives of natural science and problem formulation, and that causes should be investigated from the combined perspectives of social science, economic evaluation, and policy design and evaluation, with an awareness of the possibility of consensus building. In this study, based on the ongoing international trend of ecosystem conservation, an economic analysis was conducted to examine the direction of Satoyama development as a case study of urban and peri-urban ecosystem conservation. The result identified the preference and needs of citizens with regard to Satoyama ecosystems and discussed the consistency between policy targets and citizens’ evaluation.Springer Science and Business Media LLC, Jul. 2023, International Journal of Economic Policy Studies, 17(2) (2), 403 - 419, English[Refereed]Scientific journal
- Abstract Although environmental DNA (eDNA) metabarcoding is an exceptionally useful and powerful tool for monitoring biodiversity, little is known about whether the traits of organisms and their ecosystem characteristics affect eDNA metabarcoding performance. Nationwide surveys can provide more detailed insights, yet such studies have rarely been conducted. In order to evaluate eDNA metabarcoding performance in broad‐scale monitoring, we examined the effects of species ecological/biological traits and ecosystem characteristics on species detection rates and the implications for community analysis. In addition, we tested the effects of sample mixing and transportation methods, including cooling and freezing, on eDNA metabarcoding. On a nationwide scale, we conducted eDNA metabarcoding for fish communities in 18 Japanese lakes. By comparing species records, we observed that certain traits, including body size, body shape, saltwater tolerance and habitat preference, influenced eDNA detection. In addition, the proportion of species detected decreased significantly with an increase in lake surface area owing to ecosystem size effect on species detection. We conclude that species traits, including habitat preference, body size and ecosystem size, should be considered when assessing the eDNA metabarcoding performance in broad‐scale monitoring.Wiley, Jul. 2023, Freshwater Biology, 68(8) (8), 1346 - 1358, English[Refereed]Scientific journal
- Last, Jun. 2023, PeerJ, 11, e15431, English[Refereed]Scientific journal
- Wiley, May 2023, Freshwater Biology, 68(5) (5), 727 - 736, English[Refereed]Scientific journal
- Elsevier BV, Apr. 2023, City and Environment Interactions, 18, 100101, English[Refereed]Scientific journal
- Springer Science and Business Media LLC, Mar. 2023, Analytical Sciences, 39(5) (5), 721 - 728, English[Refereed]Scientific journal
- Brittle stars (class Ophiuroidea) are marine invertebrates comprising approximately 2,100 extant species, and are considered to constitute the most diverse taxon of the phylum Echinodermata. As a non-invasive method for monitoring biodiversity, we developed two new sets of PCR primers for metabarcoding environmental DNA (eDNA) from brittle stars. The new primer sets were designed to amplify 2 short regions of the mitochondrial 16S rRNA gene, comprising a conserved region (111–115 bp, 112 bp on average; named “16SOph1”) and a hyper-variable region (180–195 bp, 185 bp on average; named “16SOph2”) displaying interspecific variation. The performance of the primers was tested using eDNA obtained from two sources: a) rearing water of an 2.5 or 170 L aquarium tanks containing 15 brittle star species and b) from natural seawater collected around Misaki, the Pacific coast of central Japan, at depths ranging from shallow (2 m) to deep (> 200 m) sea. To build a reference library, we obtained 16S rRNA sequences of brittle star specimens collected from around Misaki and from similar depths in Japan, and sequences registered in International Nucleotide Sequence Database Collaboration. As a result of comparison of the obtained eDNA sequences with the reference library 37 (including cryptic species) and 26 brittle star species were detected with certain identities by 16SOph1 and 16SOph2 analyses, respectively. In shallow water, the number of species and reads other than the brittle stars detected with 16SOph1 was less than 10% of the total number. On the other hand, the number of brittle star species and reads detected with 16SOph2 was less than half of the total number, and the number of detected non-brittle star metazoan species ranged from 20 to 46 species across 6 to 8 phyla (only the reads at the “Tank” were less than 0.001%). The number of non-brittle star species and reads at 80 m was less than 10% with both of the primer sets. These findings suggest that 16SOph1 is specific to the brittle star and 16SOph2 is suitable for a variety of marine metazoans. It appears, however, that further optimization of primer sequences would still be necessary to avoid possible PCR dropouts from eDNA extracts. Moreover, a detailed elucidation of the brittle star fauna in the examined area, and the accurate identification of brittle star species in the current DNA databank is required.Pensoft Publishers, Feb. 2023, Metabarcoding and Metagenomics, 7, e94298, English[Refereed]Scientific journal
- Abstract In recent years, environmental DNA (eDNA) technology has been used in a variety of water environments. Environmental DNA concentrations in marine samples tend to be lower than those in freshwater samples, and few studies have explored methods to improve the recovery yields of eDNA from seawater samples. In this study, we compared different seawater preservation solutions (RNAlater or ATL) to improve eDNA yields. The eDNA concentrations of vertebrate and invertebrate species were compared using species-specific eDNA assays, and the number of detected fish and their compositions were compared using metabarcoding analysis. ATL treatment resulted in significantly higher eDNA yields for both vertebrate and invertebrate species than RNAlater treatment. Metabarcoding analysis revealed non-significant effects of preservation on the number of detected species and species composition. These results suggest that ATL treatment improves DNA yields without changing the species composition compared with the commonly used RNAlater treatment. The findings of this study will reduce false-negative outcomes and provide highly reliable results in future biological surveys. Graphical abstractLast, Springer Science and Business Media LLC, Feb. 2023, Analytical Sciences, 39(5) (5), 713 - 720, English[Refereed]Scientific journal
- Last, Wiley, Jan. 2023, Freshwater Biology, 68(1) (1), 103 - 114, English[Refereed]Scientific journal
- Springer Science and Business Media LLC, Jan. 2023, Landscape and Ecological Engineering, 19(1) (1), 11 - 19, English[Refereed]Scientific journal
- Springer Science and Business Media LLC, Jan. 2023, Landscape and Ecological Engineering, 19(1) (1), 3 - 10, English[Refereed]Scientific journal
- Last, Nov. 2022, J. JSCE, Ser. B2, Coastal Engineering, 78(2) (2), I_865 - I_870, JapaneseInfluence of flow in seagrass bed monitoring utilizing environmental ANA analysis[Refereed]Scientific journal
- Wiley, Nov. 2022, Environmental DNA, 4(6) (6), 1369 - 1380, Japanese[Refereed]Scientific journal
- Wiley, Oct. 2022, People and Nature, 4(5) (5), 1176 - 1189, English[Refereed]Scientific journal
- Sep. 2022, MicrobiologyOpen, 11(5) (5), e1317, EnglishIsolation of extended-spectrum beta-lactamase-producing Escherichia coli from Japanese red fox (Vulpes vulpes japonica)[Refereed]Scientific journal
- Last, Elsevier BV, Sep. 2022, Ecological Indicators, 142, 109213 - 109213, English[Refereed]Scientific journal
- Preventing mosquito-borne infectious diseases requires that vector mosquitoes are monitored and controlled. Targeting immature mosquitoes (eggs, larvae, and pupae), which have less mobility than adults, is an effective management approach. However, conducting these surveys is often difficult due to the limitations of morphological classification and survey costs. The application of environmental DNA (eDNA) analysis can solve these issues because it allows easy estimation of species distribution and morphology-independent species identification. Although a few previous studies have reported mosquito eDNA detection, there is a gap in knowledge regarding the dynamics related to the persistence of immature mosquito eDNA. We used Culex pipiens pallens, a vector of West Nile fever, as a model species. First, we developed a species-specific detection assay and confirmed its specificity using in silico and in vitro tests. Next, we conducted laboratory experiments using breeding tanks. Water samples were collected at each developmental stage. In addition, water samples were collected daily until the seventh day after emergence from the pupae. We quantified eDNA using real-time PCR with the developed assay to investigate the dynamics of mosquito eDNA. The specificity of the developed assay was confirmed by in silico and in vitro tests. Mosquito eDNA was detected at all developmental stages and detected up to seven days after emergence of pupae. In particular, high concentrations of eDNA were detected immediately after hatching from eggs and after emergence from pupae. Highly frequent positive eDNA signals were continuously detected between egg hatching and pupa hatching. Mosquito eDNA was detected immediately after the eggs were introduced, and eDNA-positive detections continued until pupae emergence, suggesting that eDNA analysis is useful for monitoring mosquito larvae. In the future, monitoring immature mosquitoes using eDNA analysis will contribute to prevent mosquito-borne infectious diseases.Last, Public Library of Science (PLoS), Aug. 2022, PLOS ONE, 17(8) (8), e0272653 - e0272653, English[Refereed]Scientific journal
- Aug. 2022, Water, Land and Environmental Engineering, 90(8) (8), 11 - 14, JapaneseApplicability of environmental DNA surveys in agricultural waterways and diffusion distances of environmental DNA[Refereed][Invited]Scientific journal
- Wiley, Jun. 2022, Journal of Fish Biology, 101(3) (3), 613 - 627, English[Refereed]Scientific journal
- Abstract In an era of severe biodiversity loss, biological monitoring is becoming increasingly essential. The analysis of environmental DNA (eDNA) has emerged as a new approach that could revolutionize the biological monitoring of aquatic ecosystems. Over the past decade, macro-organismal eDNA analysis has undergone significant developments and is rapidly becoming established as the golden standard for non-destructive and non-invasive biological monitoring. In this review, I summarize the development of macro-organismal eDNA analysis to date and the techniques used in this field. I also discuss the future perspective of these analytical methods in combination with sophisticated analytical techniques for DNA research developed in the fields of molecular biology and molecular genetics, including genomics, epigenomics, and single-cell technologies. eDNA analysis, which to date has been used primarily for determining the distribution of organisms, is expected to develop into a tool for elucidating the physiological state and behavior of organisms. The fusion of microbiology and macrobiology through an amalgamation of these technologies is anticipated to lead to the future development of an integrated biology.Oxford University Press (OUP), Jun. 2022, DNA Research, 29(3) (3), dsac018, English[Refereed][Invited]Scientific journal
- Mycetoma is a tropical disease caused by several fungi and bacteria present in the soil. Fungal mycetoma and eumycetoma are especially challenging to treat; therefore, prevention, early diagnosis, and early treatment are important, but it is also necessary to understand the geographic distribution of these pathogenic fungi. In this study, we used DNA metabarcoding methodology to identify fungal species from soil samples. Soil sampling was implemented at seven villages in an endemic area of Sennar State in Sudan in 2019, and ten sampling sites were selected in each village according to land-use conditions. In total, 70 soil samples were collected from ground surfaces, and DNA in the soil was extracted with a combined method of alkaline DNA extraction and a commercial soil DNA extraction kit. The region for universal primers was selected to be the ribosomal internal transcribed spacer one region for metabarcoding. After the second PCR for DNA library preparation, the amplicon-based DNA analysis was performed using next-generation sequencing with two sets of universal primers. A total of twelve mycetoma-causative fungal species were identified, including the prime agent,Public Library of Science (PLoS), Mar. 2022, PLoS Neglected Tropical Diseases, 16(3) (3), e0010274 - e0010274, English
Madurella mycetomatis , and additional pathogens,Falciformispora senegalensis andFalciformispora tompkinsii , in 53 soil samples. This study demonstrated that soil DNA metabarcoding can elucidate the presence of multiple mycetoma-causative fungi, which may contribute to accurate diagnosis for patient treatment and geographical mapping.[Refereed]Scientific journal - Last, Wiley, Mar. 2022, Environmental DNA, 4(2) (2), 271 - 283, English[Refereed][Invited]Scientific journal
- Biodiversity monitoring is important for the conservation of natural ecosystems in general, but particularly for amphibians, whose populations are pronouncedly declining. However, amphibians’ ecological traits (e.g. nocturnal or aquatic) often prevent their precise monitoring. Environmental DNA (eDNA) metabarcoding – analysis of extra-organismal DNA released into the environment – allows the easy and effective monitoring of the biodiversity of aquatic organisms. Here, we developed and tested the utility of original PCR primer sets. First, we conductedLast, Pensoft Publishers, Feb. 2022, Metabarcoding and Metagenomics, 6, 15 - 26, English
in vitro PCR amplification tests with universal primer candidates using total DNA extracted from amphibian tissues. Five primer sets successfully amplified the target DNA fragments (partial 16S rRNA gene fragments of 160–311 bp) from all 16 taxa tested (from the three living amphibian orders Anura, Caudata and Gymnophiona). Next, we investigated the taxonomic resolution retrieved using each primer set. The results revealed that the universal primer set “Amph16S” had the highest resolution amongst the tested sets. Finally, we applied Amph16S to the water samples collected in the field and evaluated its detection capability by comparing the species detected using eDNA and physical survey (capture-based sampling and visual survey) in multiple agricultural ecosystems across Japan (160 sites in 10 areas). The eDNA metabarcoding with Amph16S detected twice as many species as the physical surveys (16 vs. 8 species, respectively), indicating the effectiveness of Amph16S in biodiversity monitoring and ecological research for amphibian communities.[Refereed]Scientific journal - Feb. 2022, Knowledge and Management of Aquatic Ecosystems, 423, 4, EnglishAutumn dispersal and limited success of reproduction of the deepbody bitterling (Acheilognathus longipinnis) in terrestrialized floodplain[Refereed]Scientific journal
- Springer Science and Business Media LLC, Jan. 2022, Fisheries Science, 88(1) (1), 91 - 107, English[Refereed]Scientific journal
- Springer Science and Business Media LLC, Jan. 2022, Fisheries Science, 88(1) (1), 55 - 62, English[Refereed]Scientific journal
- Springer Science and Business Media LLC, Jan. 2022, Limnology, 23(1) (1), 9 - 16, English[Refereed]Scientific journal
- Last, Wiley, Nov. 2021, Limnology and Oceanography: Methods, 19(11) (11), 758 - 768, English[Refereed]Scientific journal
- 環境DNAを活用した藻場の増減を捉えるモニタリング方法の開発には，採水，分析手順のプロトコル化が重要である．これまでの研究で，現地調査での海草の環境DNA量は，1Lの海水を用いる従来法で定量下限以下となり，ろ過量を増やし，環境DNAを多く回収することで定量分析が可能になることを確認してきた．しかしながら，現地調査で想定される懸濁物質等により必要量をろ過できない場合の対策法は確立されていない．本研究では，低濃度で懸濁物質が多い試料から海草のDNAを多く回収する方法を検討し，現地調査に適応可能な分析方法の有効性を確認した．また，コアマモ場の入り江を対象に数値計算を実施し，採水に適した時間帯が地点毎に異なり，現地調査に向けて採水地点，時刻選定が重要であることを示した．Japan Society of Civil Engineers, Nov. 2021, Journal of Japan Society of Civil Engineers, Ser. B2 (Coastal Engineering), 77(2) (2), I_895 - I_900, Japanese[Refereed]Scientific journal
- Nov. 2021, Japanese Journal of Ichthyology, 68(2) (2), 163 - 172, JapaneseDiscovery of residual Ayu fish in the backwater area of the Yodo River Barrage[Refereed]Scientific journal
- The spread of antimicrobial-resistant bacteria (ARB) in natural environments including wild animals is a concern for public health. Birds cover large areas, and some fly across borders to migrate in large flocks. As a migratory bird, the Greater White-fronted Goose (Anser albifrons) travels to Miyajimanuma, North Japan, each spring and autumn. To investigate the ARB in migratory birds and their surroundings, we collected 110 fecal samples of A. albifrons and 18 water samples from Miyajimanuma in spring and autumn of 2019. Isolation of Escherichia coli was performed using selective agars with or without antimicrobials (cefazolin and nalidixic acid). Isolates of E. coli were recovered from 56 fecal samples (50.9%) and five water samples (27.8%) on agars without antimicrobials. No isolates were recovered on agars with antimicrobials. One E. coli isolate derived from a fecal sample exhibited resistance to β-lactams (ampicillin and cefazolin), whereas all other isolates exhibited susceptibility to all tested antimicrobials. The resistant isolate harbored blaACC, which could be transferred to other bacteria and confer resistance to β-lactams. These results suggest a low prevalence of antimicrobial resistance in wild migratory birds and their living environments; however, wild migratory birds sometimes carry ARB harboring transferrable antimicrobial resistance genes and therefore present a risk of spreading antimicrobial resistance.Wildlife Disease Association, Oct. 2021, Journal of Wildlife Diseases, 57(4) (4), 954 - 958, English, International magazine[Refereed]Scientific journal
- Wiley, Oct. 2021, Diversity and Distributions, 27(10) (10), 1958 - 1965, English[Refereed]Scientific journal
- Springer Science and Business Media LLC, Aug. 2021, Scientific Reports, 11(1) (1), 16830, Japanese
Abstract Environmental DNA (eDNA) can be a powerful tool for detecting the distribution and abundance of target species. This study aimed to test the longevity of eDNA in marine sediment through a tank experiment and to use this information to reconstruct past faunal occurrence. In the tank experiment, juvenile jack mackerel (Trachurus japonicus ) were kept in flow-through tanks with marine sediment for two weeks. Water and sediment samples from the tanks were collected after the removal of fish. In the field trial, sediment cores were collected in Moune Bay, northeast Japan, where unusual blooms of jellyfish (Aurelia sp.) occurred after a tsunami. The samples were analyzed by layers to detect the eDNA of jellyfish. The tank experiment revealed that after fish were removed, eDNA was not present in the water the next day, or subsequently, whereas eDNA was detectable in the sediment for 12 months. In the sediment core samples, jellyfish eDNA was detected at high concentrations above the layer with the highest content of polycyclic aromatic hydrocarbons, reflecting tsunami-induced oil spills. Thus, marine sediment eDNA preserves a record of target species for at least one year and can be used to reconstruct past faunal occurrence.[Refereed]Scientific journal - Springer Science and Business Media LLC, Aug. 2021, Limnology, 22(3) (3), 363 - 370, English[Refereed]Scientific journal
- Understanding the processes of environmental DNA (eDNA) persistence and degradation is essential to determine the spatiotemporal scale of eDNA signals and accurately estimate species distribution. The effects of environmental factors on eDNA persistence have previously been examined; however, the influence of the physiochemical and molecular states of eDNA on its persistence is not completely understood. Here, we performed meta-analyses including 26 previously published papers on the estimation of first-order eDNA decay rate constants, and assessed the effects of filter pore size, DNA fragment size, target gene, and environmental conditions on eDNA decay rates. Almost all supported models included the interactions between the filter pore size and water temperature, between the target gene and water temperature, and between the target gene and water source, implying the influence of complex interactions between the eDNA state and environmental conditions on eDNA persistence. These findings were generally consistent with the results of a re-analysis of a previous tank experiment which measured the time-series changes in marine fish eDNA concentrations in multiple size fractions after fish removal. Our results suggest that the mechanism of eDNA persistence and degradation cannot be fully understood without knowing not only environmental factors but also cellular and molecular states of eDNA in water. Further verification of the relationship between eDNA state and persistence is required by obtaining more information on eDNA persistence in various experimental and environmental conditions, which will enhance our knowledge on eDNA persistence and support our findings.Last, Jul. 2021, Molecular Ecology Resources, 21(5) (5), 1490 - 1503, English, International magazine[Refereed]Scientific journal
- Wiley, Jul. 2021, Molecular Ecology, 30(13) (13), 3057 - 3067, English[Refereed]Scientific journal
- Last, Wiley, Jun. 2021, Ecosphere, 12(6) (6), e03643, English[Refereed]Scientific journal
- Springer Science and Business Media LLC, May 2021, Scientific Reports, 11(1) (1), 10712, English
Abstract Alien ant species (Formicidae, Hymenoptera) cause serious damage worldwide. Early detection of invasion and rapid management are significant for controlling these species. However, these attempts are sometimes hindered by the need for direct detection techniques, such as capture, visual observation, or morphological identification. In this study, we demonstrated that environmental DNA (eDNA) analysis can be used as a monitoring tool for alien ants usingLinepithema humile (Argentine ant), one of the most invasive ants, as a model species. We designed a new real-time PCR assay specific toL. humile and successfully detected eDNA from the surface soil. The reliability of eDNA analysis was substantiated by comparing eDNA detection results with traditional survey results. Additionally, we examined the relationship between eDNA concentration and distance from nests and trails. Our results support the effectiveness of eDNA for alien ant monitoring and suggest that this new method could improve our ability to detect invasive ant species.[Refereed]Scientific journal - Springer Science and Business Media LLC, May 2021, Scientific Reports, 11(1) (1), 9943, English
Abstract A lack of reliable tools for determining the presence and distribution of fish species can impede understanding of predator–prey interactions and fishery management. Conventional fish survey methods are invasive, and can be size or species selective. Combining netting and electrofishing is a current method used to monitor fish species in Phayao Lake (Kwan Phayao), Thailand. However, the methods are inefficient and time-consuming. Recently, locals who rely on inland fisheries in Kwan Phayao expressed their deep concerns about the giant snakehead,Channa micropeltes (Cuvier, 1831) destroying other fish there. The giant snakehead prey on many commercially important fish species, as the prey species is reduced, negative effects on both biodiversity and the fishery sector could follow. Here, an eDNA-based survey was developed to detect the presence of the giant snakehead. Water samples were collected from six sites within Kwan Phayao and 17 sites in Ing River where water flowed into and out of Kwan Payao. The eDNA of the giant snakehead was detected in water samples from all collection sites using the developed qPCR assay with various concentrations. The eDNA was shown here to be a sensitive and reliable tool for fish surveillance so there will be a better chance for developing an effective management strategy.[Refereed]Scientific journal - Outbreaks of bacterial cold-water disease (BCWD), caused by Flavobacterium psychrophilum, are widespread in Japan, especially among ayu Plecoglossus altivelis. There are few investigations of F. psychrophilum in river water, and its seasonal distribution has not been clarified. We aimed to identify the spatiotemporal dynamics of F. psychrophilum and ayu to provide information that is useful for establishing a countermeasure for BCWD. Quantitative analysis of environmental DNA (eDNA) was used to clarify the year-round dynamics of ayu and F. psychrophilum. We sampled river water from the Nagara and Ibi rivers in Japan, and conducted monthly water sampling and eDNA quantification. Changes in the eDNA concentration of ayu were consistent with the known life histories of the fish. There was a strong negative correlation between the eDNA concentration of F. psychrophilum and water temperature, suggesting a strong dependence of F. psychrophilum dynamics in the river on water temperature. Furthermore, relatively high eDNA concentrations were recorded for both organisms in early summer and fall, suggesting that ayu is infected with F. psychrophilum during these seasons when experiencing up- and downmigration, respectively.Springer Science and Business Media LLC, May 2021, Fisheries Science, 87(3) (3), 321 - 330, English[Refereed]Scientific journal
- Wiley, May 2021, Ecological Research, 36(3) (3), 379 - 388, English[Refereed]Scientific journal
- In recent years, biodiversity loss has become one of the most serious environmental issues worldwide, especially in aquatic ecosystems. To avoid diversity loss, it is necessary to monitor biological communities, and environmental DNA (eDNA) metabarcoding has been developed as a rapid, noninvasive, and cost-effective method for aquatic biodiversity monitoring. Although this method has been applied to various environments and taxa, a detailed assessment of the efficient sampling methods for monitoring is still required. In this study, we explored eDNA metabarcoding sampling methods for fish at a single site to maximize the number of detected species using realistic effort in a natural, small river. We considered the following three parameters: sample type (water or sediment), sample position at a site (right and left shore and center of the river), and water volume (10-4000 mL). The results suggested that the number of detected species from sedimentary eDNA was equivalent to that from aqueous eDNA, although the species composition was different. The number of detected species could be saturated by collecting a 1000 mL water sample, regardless of sampling position within a survey site. However, sedimentary eDNA showed a spatially heterogeneous species composition between sampling positions within a survey site despite the short distance (5 m) between positions, without apparent differences in physical properties such as velocity and sediment particle distribution. By completing eDNA biodiversity monitoring of fish with 1000 mL water samples across the whole river, we detected more fish species than in previous traditional surveys conducted at the same sites. Thus, the aqueous eDNA metabarcoding method is as efficient as traditional surveys, while sedimentary eDNA metabarcoding could complement the results of aqueous eDNA metabarcoding.Last, Springer Science and Business Media LLC, Apr. 2021, Limnology, 22(2) (2), 221 - 235, English[Refereed]Scientific journal
- Springer Science and Business Media LLC, Feb. 2021, Scientific Reports, 11(1) (1), 4372, English
Abstract The combination of high-throughput sequencing technology and environmental DNA (eDNA) analysis has the potential to be a powerful tool for comprehensive, non-invasive monitoring of species in the environment. To understand the correlation between the abundance of eDNA and that of species in natural environments, we have to obtain quantitative eDNA data, usually via individual assays for each species. The recently developed quantitative sequencing (qSeq) technique enables simultaneous phylogenetic identification and quantification of individual species by counting random tags added to the 5′ end of the target sequence during the first DNA synthesis. Here, we applied qSeq to eDNA analysis to test its effectiveness in biodiversity monitoring. eDNA was extracted from water samples taken over 4 days from aquaria containing five fish species (Hemigrammocypris neglectus ,Candidia temminckii ,Oryzias latipes ,Rhinogobius flumineus , andMisgurnus anguillicaudatus ), and quantified by qSeq and microfluidic digital PCR (dPCR) using a TaqMan probe. The eDNA abundance quantified by qSeq was consistent with that quantified by dPCR for each fish species at each sampling time. The correlation coefficients between qSeq and dPCR were 0.643, 0.859, and 0.786 forH. neglectus ,O. latipes , andM. anguillicaudatus , respectively, indicating that qSeq accurately quantifies fish eDNA.[Refereed]Scientific journal - Wiley, Feb. 2021, Microbiology and Immunology, 65(2) (2), 99 - 100, English, International magazineScientific journal
- Lead, Springer Science and Business Media LLC, Feb. 2021, Biological Invasions, 23(2) (2), 507 - 520, English[Refereed]Scientific journal
- Wiley, Jan. 2021, Environmental DNA, 3(1) (1), 14 - 21, English[Refereed]Scientific journal
- Environmental DNA (eDNA)-based assessments of macro-organisms have now become an essential approach for biomonitoring. eDNA survey methods have a number of advantages over conventional survey methods. However, the value of the data that will accumulate would be greatly enhanced by standardizing the analysis methods, which would allow us to compare data from multiple monitoring sites at different points in time. The eDNA Society (http://ednasociety.org/en/about), whose founding members consist of Japanese researchers conducting eDNA studies on macro-organisms, was established in 2018, with the aim of expanding eDNA technology and science. Here, we introduce our key publication, “Environmental DNA Sampling and Experiment Manual” (http://ednasociety.org/en/manual), which was published under the initiative of the eDNA Society. Detailed methods for the surveys and experiments are described in the manual, including the selection of sampling sites, sampling methods, filtration methods, DNA extraction, species-specific detection by real-time polymerase chain reaction, and fish eDNA metabarcoding. The manual assists users in conducting standardized surveys and quality experiments, and provides a basis for collecting comparable data. Given that the efficacy of methods can be context dependent and variable, and that procedures may sometimes conflict with standardization, it is difficult to ensure that all processes are equally effective. However, even in such cases, it is important to maintain sufficiently high data quality by setting the minimum standards to be followed. Implementation of such standardized methodologies will enable the systematic and frequent collection of flawless, comparable eDNA data from around the world; this will provide important fundamental information for biodiversity conservation, as well as the sustainable use of fisheries resources.Wiley, Jan. 2021, Environmental DNA, 3(1) (1), 8 - 13, English[Refereed]Scientific journal
- Springer Science and Business Media LLC, Jan. 2021, Ichthyological Research, 68(1) (1), 152 - 157, English[Refereed]Scientific journal
- ResearchersLinks Ltd, Jan. 2021, Pakistan Journal of Zoology, 53(1) (1), 253 - 272, English[Refereed]Scientific journal
- Springer Science and Business Media LLC, Jan. 2021, Journal of Ocean University of China, 20(1) (1), 124 - 136, English[Refereed]Scientific journal
- Last, Dec. 2020, PeerJ, 8, e10338, EnglishA molecular survey based on eDNA to assess the presence of a clown featherback (Chitala ornata) in a confined environment[Refereed]Scientific journal
- The diversity and the abundance of amphibians have dramatically declined globally over the past 30 years, and the monitoring and conservation of their habitats is essential. However, traditional methods such as bait trapping and mark-recapture are costly, and morphological identification usually requires a high level of taxonomic expertise. Here, seasonal surveillances of Hida salamanderInter-Research Science Center, Nov. 2020, Endangered Species Research, 43, 341 - 352, English
Hynobius kimurae were performed by means of environmental DNA (eDNA) analysis withHynobius -specific primers and a species-specific TaqMan probe. Water sampling and visual surveys were conducted seasonally in a stream in Kyoto Prefecture, Japan. Detection rates of eDNA were then calculated by real-time PCR, and eDNA site occupancy probability was estimated by multi-scale occupancy modeling. The eDNA-based detection rate of Hida salamander was 76.7%, whereas the visual survey-based detection rate was 23.3%, and target eDNA was detected at almost all sites where the presence of target species was visually confirmed. Moreover, factors relating to the site- and sample-level occurrence probabilities of the target eDNA differed depending on the developmental stage of the target species. Our findings support previous studies showing that eDNA analysis enables an effective assessment of amphibian distributions without damaging the organisms or their habitat, and we compare for the first time the site occupancy probability of amphibian eDNA throughout the life cycle of an amphibian species. The present study contributes to the development of eDNA analysis as a tool for understanding the distribution and seasonal activity of amphibian species and will thus aid in the planning of conservation measures and habitat restoration for these species.[Refereed]Scientific journal - Last, Japan Society of Civil Engineers, Nov. 2020, Journal of Japan Society of Civil Engineers, Ser. B2 (Coastal Engineering), 76(2) (2), I_949 - I_954, English[Refereed]Scientific journal
- Last, Japan Society of Civil Engineers, Nov. 2020, Journal of Japan Society of Civil Engineers, Ser. B2 (Coastal Engineering), 76(2) (2), I_943 - I_948, Japanese
The utilization of environmental DNA(eDNA) is being considered as a new method for monitoring seagrass beds. To understand the change in eDNA corresponding to seasonal variations in seagrass, the growth of Zostera marina inhabiting inside a water tank along with the amount of eDNA were investigated for fifteen months. Furthermore, investigations were performed under conditions where the flow changes due to the tide for the future monitoting in the sea. The investigations focused on the differernce of the eDNA collected at different sampling points and at the same time of a day on the seasonal changes.
The fifteen month monitoring confirmed that the concentration of eDNA was high in the summer season which suggests that it is related to the increase in the amount of seagrass biomass and its release. As a result of the survey in the sea, the samples taken at the same time of a day showed a mixture of detection and non-detection. However, by increasing the amount of water sampled by 10 times, and the amount of analysis by 2.5times, and also by reducing the inhibition effect, detection was possible. Additionally, the results showed the possibility of applying the method in this study for future surveys in the sea.
[Refereed]Scientific journal - In freshwater ecosystems, invasive salmonid fishes can have a significant impact on native fish species. Detecting the invasion and its negative effects is critical for the conservation of native fish communities. We examined the species composition and seasonal changes in the freshwater fish community, including salmonids, on the Kamikawa Plain, Hokkaido Island, Japan, using environmental DNA (eDNA) metabarcoding. We detected 23 fish species in 176 samples collected from 16 sites over 12 months (October 2018 – August 2019). Between 11 and 20 species were detected at each site, including five native salmonids (Last, Pensoft Publishers, Oct. 2020, Biodiversity Data Journal, 8, e56876, English, International magazine
Oncorhynchus masou ,Oncorhynchus keta ,Parahucho perryi ,Salvelinus leucomaenis leucomaenis andSalvelinus malma krascheninnikova ). The invasive alien rainbow troutOncorhynchus mykiss was detected at all 16 sites and it was the most commonly detected salmonid. Although we found no obvious competitive exclusion of native salmonids by rainbow trout in the study area, the invasive species occurred more often and at more sites than any of the natives. We also determined the occurrence and seasonal changes in the fish community, classified as native salmonids, invasive rainbow trout, Cypriniformes and other benthic fishes. There were fewer species overall in winter, but the sites with higher species richness in winter were on the lower reaches of the river. In addition, we detected domestic invaders, such as the topmouth gudgeon,Pseudorasbora parva , although they were less prevalent than rainbow trout. These results show the effectiveness of eDNA metabarcoding, which can be used for surveying species richness at an ecosystem scale. In particular, the detection of the early stages of establishment and spread of invasive species can be achieved by eDNA monitoring.[Refereed]Scientific journal - Springer Science and Business Media LLC, Oct. 2020, Communications Biology, 3(1) (1), 558, English
Abstract Far too little is known about the long-term dynamics of populations for almost all macro-organisms. Here, we examined the utility of sedimentary DNA techniques to reconstruct the dynamics in the “abundance” of a species, which has not been previously defined. We used fish DNA in marine sediments and examined whether it could be used to track the past dynamics of pelagic fish abundance in marine waters. Quantitative PCR for sedimentary DNA was applied on sediment-core samples collected from anoxic bottom sediments in Beppu Bay, Japan. The DNA of three dominant fish species (anchovy, sardine, and jack mackerel) were quantified in sediment sequences spanning the last 300 years. Temporal changes in fish DNA concentrations are consistent with those of landings in Japan for all three species and with those of sardine fish scale concentrations. Thus, sedimentary DNA could be used to track decadal-centennial dynamics of fish abundance in marine waters.[Refereed]Scientific journal - Wiley, Oct. 2020, Environmental DNA, 2(4) (4), 627 - 634, English[Refereed]Scientific journal
- Aqueous environmental DNA (eDNA) analysis has been applied to the monitoring of various ecosystems and taxa, and the characteristics of aqueous eDNA have been previously studied. In contrast, although sedimentary eDNA has been used to restore past information, the characteristics of sedimentary eDNA are not well understood. In this study, we compared the properties of sedimentary and aqueous eDNA of macro-organisms. First, to clarify the preservation ability of sediments, we compared the difference in decay rates between aqueous and sedimentary eDNA using samples collected from a biotope (an artificial pond prepared with concrete). Next, to clarify the biological information retained in sedimentary eDNA both qualitatively and quantitatively, we compared eDNA concentrations between sediment and water samples collected simultaneously from a lake, and the fish species detected by eDNA metabarcoding were also compared. The results demonstrated the following: (a) the decay rate (decreased eDNA copy number divided by the initial eDNA copy number per unit time) of sedimentary eDNA (0.00033 ± 0.000049 [mean ± SE]/hr) was lower than that of aqueous eDNA (0.01863 ± 0.0011/hr); (b) sedimentary eDNA concentration of the mitochondrial marker of three fish species was higher than aqueous eDNA concentration for the same sample weight (12.5–1,456.9 times); and (c) the species composition obtained by metabarcoding was not significantly different between sediment and water; however, considering the lower decay rate of sedimentary eDNA, using both sample types may provide more comprehensive information of species distribution. Thus, sedimentary eDNA analysis will expand future biomonitoring and ecological studies by providing a difference in timescale.Last, Wiley, Oct. 2020, Environmental DNA, 2(4) (4), 505 - 518, English[Refereed]Scientific journal
- Last, Elsevier BV, Sep. 2020, Science of The Total Environment, 735, 139462 - 139462, English[Refereed]Scientific journal
- Wiley, Sep. 2020, Molecular Ecology Resources, 20(5) (5), 1248 - 1258, English[Refereed]Scientific journal
- PeerJ, Aug. 2020, PeerJ, 8, e9764 - e9764, English
Background Freshwater ecosystems are rapidly declining. The Siberian salamander (Salamandrella keyserlingii ) which inhabits the Kushiro marsh in Hokkaido, Japan has lost some habitat due to human activity. There are many challenges associated with conventional monitoring methods, including cost, the need for specialist personnel, environmental impact, and ability to detect the presence of this species; thus, we investigated the feasibility of using environmental DNA (eDNA) analysis to detect its presence and identify its breeding grounds.Methods We performed tank experiments to confirm eDNA emission from egg sacs, larvae, and adult Siberian salamanders in the water. We also performed water sampling and visual observation of egg sacs in the Kushiro marsh during the end of the breeding season and the larval season.Results The tank experiments found eDNA emission from all growth stages. It also implied concentrated emissions just after spawning and after hatching, and limited emissions during the incubation phase in egg sacs. We also detected eDNA in the field, likely reflecting the distribution of egg sacs or larvae. Combining this data with visual observations, it was determined that the eDNA results from the field were best explained by the number of egg sacs within 7–10 m of the sampling point.Conclusions The results of this investigation show that the breeding sites and habitats of marshland species can successfully be monitored using eDNA analysis. They also suggest that the eDNA results from the marshes may reflect the biomass that is in close range to the sampling point. These results support the increased use of eDNA analysis in marshes and provide knowledge that could improve the interpretation of future results.[Refereed]Scientific journal - Wiley, Jul. 2020, Limnology and Oceanography: Methods, 18(8) (8), 437 - 445, English[Refereed]Scientific journal
- Although populations of anguillid eels have declined remarkably in recent decades, monitoring data on the spatial and temporal variation in their dynamics are often limited, particularly for tropical eel species. As there are often sympatries of multiple eel species in tropical rivers, identifying eel species based solely on morphological characteristics is challenging. Basin-scale surveys were conducted in rivers of southern Japan and northern Taiwan to investigate (1) whether the spatial distribution, abundance, and biomass of the tropical eel species, the giant mottled eel (Anguilla marmorata), can be monitored in rivers by comparing the results obtained from environmental DNA (eDNA) analysis with data from electrofishing and (2) the riverine distribution of the sympatric A. marmorata and the temperate eel species, the Japanese eel (Anguilla japonica), in this region using eDNA analysis. Although we found an much lower abundance of A. marmorata in the study region, we identified the eDNA of the species from all of the study sites (21 sites) where it was collected by electrofishing, in addition to 22 further study sites where it was not collected directly. This indicates that eDNA analysis has a greater sensitivity for detecting A. marmorata, making it a powerful tool for monitoring the spatial distribution of the species in rivers. We found a significant positive relationship between eDNA concentration and both the abundance and biomass of A. marmorata, and eDNA concentration seemed to better reflect the abundance of the species than did biomass. eDNA of both A. japonica and A. marmorata was identified from almost all rivers, indicating the sympatry of these species in this region, although the degree of sympatry differed between rivers. Though the eDNA concentration of A. japonica decreased significantly with increasing distance from the river mouth, no significant relationship was found for A. marmorata. This study is the first to demonstrate the potential usefulness of eDNA analysis for estimating the spatial distribution, abundance, and biomass of tropical eels in rivers and to further apply this method to investigate sympatry among anguillid species. eDNA analysis can help in obtaining data on the population dynamics of tropical eels, providing invaluable information for managing these species.Jun. 2020, Zoological Studies, 59, 17, English, International magazine[Refereed]Scientific journal
- Last, Wiley, May 2020, Ecology and Evolution, 10(11) (11), 5354 - 5367, English[Refereed]Scientific journal
- Water sampling and filtration of environmental DNA (eDNA) analysis have been performed by several different methods, and each method may yield a different species composition or eDNA concentration. Here, we investigated the eDNA of seawater samples directly collected by SCUBA to compare two widely used filtration methods: open filtration with a glass filter (GF/F) and enclosed filtration (Sterivex). We referred to biomass based on visual observation data collected simultaneously to clarify the difference between organism groups. Water samples were collected at two points in the Sea of Japan in May, September and December 2018. The respective samples were filtered through GF/F and Sterivex for eDNA extraction. We quantified the eDNA concentration of five fish and two cnidarian species by quantitative polymerase chain reaction (qPCR) using species-specific primers/probe sets. A strong correlation of eDNA concentration was obtained between GF/F and Sterivex; the intercepts and slopes of the linear regression lines were slightly different in fish and jellyfish. The amount of eDNA detected using the GF/F filtration method was higher than that detected using Sterivex when the eDNA concentration was high; the opposite trend was observed when the eDNA concentration was relatively low. The concentration of eDNA correlated with visually estimated biomass; eDNA concentration per biomass in jellyfish was approximately 700 times greater than that in fish. We conclude that GF/F provides an advantage in collecting a large amount of eDNA, whereas Sterivex offers superior eDNA sensitivity. Both filtration methods are effective in estimating the spatiotemporal biomass size of target marine species.Apr. 2020, PLOS ONE, 15(4) (4), e0231718, English, International magazine[Refereed]Scientific journal
- Wiley, Apr. 2020, Environmental DNA, 2(2) (2), 140 - 151, English[Refereed]Scientific journal
- Wiley, Apr. 2020, Environmental DNA, 2(2) (2), 130 - 139[Refereed]Scientific journal
- Environmental DNA (eDNA) analysis has been used as a cost-efficient and non-invasive tool for monitoring invasive and threatened species. Previous studies typically involve two approaches; species-specific detection via PCR and multiple species detection via metabarcoding. However, the former could be costly when several species are targeted, and the latter could sometimes be insufficient to distinguish closely related species. Here, the simultaneous eDNA detection from multiple species via multiplex real-time PCR was applied to 99 ponds to evaluate the distribution of three exotic and three threatened native fish species over different seasons. We detected bluegill sunfish (Lepomis macrochirus) eDNA at 31 sites, largemouth bass (Micropterus salmoides) eDNA at 22 sites, smallmouth bass (Micropterus dolomieu) eDNA at one site, golden venus chub (Hemigrammocypris rasborella) eDNA at 11 sites, Japanese medaka (Oryzias latipes) eDNA at 26 sites, and weather loach (Misgurnus anguillicaudatus) eDNA at 41 sites. We found that eDNA detection rates were higher in early summer for all fish species. Moreover, exotic fish eDNA was detected more frequently in ponds which were easier to access by car and which have a larger surface area and higher pH. Furthermore, the detection rates of native fish eDNA were generally lower in the ponds where exotic fish eDNA was detected more frequently. Multiplex real-time PCR can help detect the distribution of exotic and threatened native species for conservation and ecosystem management. This method is expected to substantially contribute to the early detection of invasive species and the efficient protection of threatened species' habitat.SPRINGER, Feb. 2020, Biological Invasions, 22(2) (2), 455 - 471, English[Refereed]Scientific journal
- Jan. 2020, Acta Hydrobiologica Sinica, 44(1) (1), ChineseThe eDNA Collection method of Zhoushan coastal waters[Refereed]Scientific journal
- Wiley, Jan. 2020, Environmental DNA, 2(1) (1), 42 - 52, English[Refereed]Scientific journal
- Environmental DNA (eDNA) is a powerful tool for monitoring the distribution of aquatic macro-organisms. However, environmental factors, including the water temperature and water quality, can affect the inhibition and/or degradation of eDNA, which complicates accurate estimations of eDNA concentrations and the detection of the presence/absence of species in natural habitats. Further very few eDNA studies have been conducted for reptiles, especially with respect to estimating their biomass and/or abundances. Here we examined the relationship between the visually-observed number of red-eared sliders (PeerJ, Dec. 2019, PeerJ, 7, e8155 - e8155, English
Trachemys scripta elegans ) and eDNA concentrations across 100 ponds. Additionally, we evaluated the effect of water quality on red-eared slider eDNA concentration in these ponds. We found that there was a significant positive correlation between the observed number of red-eared sliders and the eDNA concentration in the ponds. On comparing various water quality indicators, including dissolved nitrogen, dissolved phosphorous, organic matter, and chlorophyll a (Chl.a ), we found that only Chl.a had a negative correlation with the red-eared slider eDNA concentration, while we did not find any inhibition in the quantitative PCR. We conclude that concentrations of eDNA can potentially be used for estimating the abundance of the red-eared slider. Additionally, Chl.a might indirectly influence the degradation of eDNA through the microorganisms bonded to the phytoplankton in the ponds, as microbial activity is thought to decrease eDNA persistence.[Refereed]Scientific journal - Springer Science and Business Media LLC, Dec. 2019, Scientific Reports, 9(1) (1), 6575, English[Refereed]Scientific journal
- In recent years, the prevalence and infection intensity of Schistosoma japonicum in endemic areas of the Philippines have significantly decreased due to yearly population-based treatment strategies, yet transmission rates remain high and uninterrupted. An important indicator of active disease transmission is the presence of Schistosoma japonicum and its snail intermediate host Oncomelania hupensis quadrasi in freshwater habitats. In this study, we sought to apply a species-specific real-time PCR (qPCR) assay for the detection of S. japonicum and O. hupensis quadrasi in freshwater samples using environmental DNA approach that can complement the commonly utilized malacological survey in determining potential transmission foci in order to have a more effective snail surveillance strategy for schistosomiasis japonica in endemic areas. The newly developed assay was specific to S. japonicum and O. hupensis quadrasi with no amplification detected against non-target trematode Fasciola spp. and snails such as Lymnaea spp., Pomacea canaliculata, and Melanoides spp. that typically co-exist in the same environment. The assay effectiveness was determined using 19 environmental water samples collected from Northern Samar (N = 5 sites), Leyte (N = 11 sites) and Compostela Valley (N = 3 sites) and compared to malacological survey for determining O. hupensis quadrasi snail colonies and snail crushing to visualize S. japonicum cercariae. TaqMan qPCR targeting a short fragment of the cytochrome c oxidase subunit 1 (cox1) gene was positive for S. japonicum in 9 sites, for O. hupensis quadrasi in 9 sites, and for both S. japonicum and O. hupensis quadrasi in 5 sampling sites. Moreover, it was able to detect O. hupensis quadrasi in 3 out of 12 sites found negative and 6 out of 7 sites found positive through malacological survey, and in 4 of the 5 snail sites positive for snails with cercariae. Overall, this method can complement malacological surveys for monitoring of schistosomes in endemic areas of the Philippines, especially those with high risk of human infection.Nov. 2019, PLOS ONE, 14(11) (11), e0224617, English, International magazine[Refereed]Scientific journal
- Wiley, Nov. 2019, Environmental DNA, 1(4) (4), 359 - 367, English[Refereed]Scientific journal
- Last, Nov. 2019, Journal of Japan Society of Civil Engineers (B2), 75(2) (2), I_1087 - I_1092, Japanese[Refereed]Scientific journal
- Last, 土木学会, Nov. 2019, Journal of Japan Society of Civil Engineers (B2), 75(2) (2), I_1075 - 1080, Japanese[Refereed]Scientific journal
- Background: The perpetuation of schistosomiasis japonica in the Philippines depends to a major extent on the persistence of its intermediate host Oncomelania hupensis quadrasi, an amphibious snail. While the malacological survey remains the method of choice in determining the contamination of the environment as evidenced by snails infected with schistosome larval stages, an emerging technology known as environmental DNA (eDNA) detection provides an alternative method. Previous reports showed that O. hupensis quadrasi eDNA could be detected in water, but no reports have been made on its detection in soil. Methods: This study, thus focused on the detection of O. hupensis quadrasi eDNA from soil samples collected from two selected schistosomiasis-endemic barangays in Gonzaga, Cagayan Valley using conventional and TaqMan-quantitative (qPCR) PCRs. Results: The results show that qPCR could better detect O. hupensis quadrasi eDNA in soil than the conventional method. In determining the possible distribution range of the snail, basic edaphic factors were measured and correlated with the presence of eDNA. The eDNA detection probability increases as the pH, phosphorous, zinc, copper, and potassium content increases, possibly indicating the conditions in the environment that favor the presence of the snails. A map was generated to show the probable extent of the distribution of the snails away from the body of the freshwater. Conclusion: The information generated from this study could be used to determine snail habitats that could be possible hotspots of transmission and should, therefore, be targeted for snail control or be fenced off from human and animal contact or from the contamination of feces by being a dumping site for domestic wastes.MDPI AG, Sep. 2019, Pathogens, 8(4) (4), 160 - 160, English[Refereed]Scientific journal
- Sep. 2019, Freshwater Science, 38, 654 - 660, English[Refereed]Scientific journal
- Wiley, Sep. 2019, Environmental DNA, 1(3) (3), 281 - 289, English[Refereed]Scientific journal
- Environmental DNA (eDNA) analyses have enabled a more efficient surveillance of species distribution and composition than conventional methods. However, the characteristics and dynamics of eDNA (e.g., origin, state, transport, and fate) remain unknown. This is especially limited for the eDNA derived from nuclei (nu-eDNA), which has recently been used in eDNA analyses. Here, we compared the particle size distribution (PSD) of nu-eDNA from Japanese Jack Mackerel (Trachurus japonicus) with that of mt-eDNA (eDNA derived from mitochondria) reported in previous studies. We repeatedly sampled rearing water from the tanks under multiple temperatures and fish biomass levels, and quantified the copy numbers of size-fractioned nu-eDNA. We found that the concentration of nu-eDNA was higher than that of mt-eDNA at 3-10 mu m size fraction. Moreover, at the 0.8-3 mu m and 0.4-0.8 mu m size fractions, eDNA concentrations of both types increased with higher temperature and their degradation tended to be suppressed. These results imply that the production of eDNA from large to small size fractions could buffer the degradation of small-sized eDNA, which could improve its persistence in water. Our findings will contribute to refine the difference between nu- and mt-eDNA properties, and assist eDNA analyses as an efficient tool for the conservation of aquatic species.Last, AMER CHEMICAL SOC, Aug. 2019, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 53(16) (16), 9947 - 9956, English[Refereed]Scientific journal
- Wiley, Jul. 2019, Journal of Fish Biology, 95(3) (3), 979 - 981, English[Refereed]Scientific journal
- This study investigates how different segments of the public, with varying degrees of interest in S&T, could formulate opinions on a broader vision and the role they think STI should play in Japanese society through 2020 (Tokyo's Olympic and Paralympic year) and toward 2030. We conducted nine inclusive public engagement activities. Results indicated that the broad public opinions did not completely overlap with officials' opinions, a value of “open and appropriate” was mainly found from the unengaged public, and the visions and values based on their opinions could well be incorporated into the official document. Engaging the disinterested in S&T remains an issue.Sissa Medialab, Jun. 2019, Journal of Science Communication, 18(03) (03), A02, English[Refereed]Scientific journal
- We investigated the distributions of two native (Dolly Varden Salvelinus malma malma and whitespotted char Salvelinus leucomaenis leucomaenis) and one invasive (rainbow trout Oncorhynchus mykiss) salmonid species using environmental DNA (eDNA) analysis in the centre of Hokkaido Island, Japan. The native species' populations are fragmented by damming and threatened by invasive species. Therefore, DNA real-time PCR assays specific to these three salmonids were used to investigate the effects of damming and invasive species on the two native salmonids. Salvelinus malma populations exhibited separation due to damming. Additionally, they were not eliminated by invasive O. mykiss but rather lived together at some sites. Salvelinus leucomaenis populations occupied the lower reaches more than did S. malma populations. We detected S. leucomaenis and the invasive O. mykiss population less frequently than expected. We were unable to clarify the seasonal movements of species, even during their reproductive phase, despite conducting eDNA surveys throughout the year, including during the coldest parts of winter. We hypothesise that damming may function both as a protective barrier against invasive species and as an impassable barrier preventing migration; however, the significance of these potential functions was not revealed in this study. From a long-term perspective, fragmentation may negatively affect the viability of native Salvelinus populations. Conservation efforts for native Salvelinus species would be aided by additional studies using eDNA surveys, which can be effectively conducted even in mid-winter.Last, The Ecological Society of Japan, May 2019, Japanese Journal of Conservation Ecology, 24(1) (1), 71 - 81, Japanese[Refereed]Scientific journal
- Last, Wiley, May 2019, Environmental DNA, 1(1) (1), 54 - 63, English[Refereed]Scientific journal
- Apr. 2019, Ecosphere, 10(4) (4), e02628, English[Refereed]Scientific journal
- Mar. 2019, Estuarine, Coastal and Shelf Science, 221, 15 - 20, English[Refereed]Scientific journal
- Wiley, Mar. 2019, Aquatic Conservation: Marine and Freshwater Ecosystems, 29(3) (3), 361 - 373, English[Refereed]Scientific journal
- Springer Science and Business Media LLC, Mar. 2019, Fisheries Science, 85(2) (2), 327 - 337, English[Refereed]Scientific journal
- Environmental DNA (eDNA) analysis has successfully detected organisms in various aquatic environments. However, there is little basic information on eDNA, including the eDNA shedding and degradation processes. This study focused on water temperature and fish biomass and showed that eDNA shedding, degradation, and size distribution varied depending on water temperature and fish biomass. The tank experiments consisted of four temperature levels and three fish biomass levels. The total eDNA and size-fractioned eDNA from Japanese Jack Mackerels (Trachurus japonicus) were quantified before and after removing the fish. The results showed that the eDNA shedding rate increased at higher water temperature and larger fish biomass, and the eDNA decay rate also increased at higher temperature and fish biomass. In addition, the small-sized eDNA fractions were proportionally larger at higher temperatures, and these proportions varied among fish biomass. After removing the fish from the tanks, the percentage of eDNA temporally decreased when the eDNA size fraction was >10 mu m, while the smaller size fractions increased. These results have the potential to make the use of eDNA analysis more widespread in the future.Last, WILEY, Feb. 2019, ECOLOGY AND EVOLUTION, 9(3) (3), 1135 - 1146, English[Refereed]Scientific journal
- Dolly Varden (Salvelinus malma) and Whitespotted Char (Salvelinus leucomaenis) are representative native fish of the family Salmonidae that inhabit the upper reaches of rivers on Hokkaido Island, Japan. They are threatened by the invasive Rainbow Trout (Oncorhynchus mykiss). In this study, environmental DNA (eDNA) real-time polymerase chain reaction (PCR) assays to detect these three salmonids were developed and used to clarify the distribution pattern of these fish. A specificity test for each assay was conducted using DNA extracted from both target and closely related fish, and the specificity of each assay was confirmed. Then, we carried out eDNA surveys in two mountainous rivers around Mt. Daisetsu in winter, when snow depth was maximized. In the winter surveys, eDNA of all three species were successfully detected from river water samples, including under-ice water samples. The results of eDNA detection corresponded with the results of an earlier distribution survey performed with Japanese-style fly-fishing and lure-fishing. These results suggested that the eDNA assays developed in this study are applicable for inter-seasonal surveys for these species.WILEY, Jan. 2019, Ecological Research, 34(1) (1), 237 - 242, English[Refereed]Scientific journal
- Inter-Research Science Center, Jan. 2019, Marine Ecology Progress Series, 609, 187 - 196, English[Refereed]Scientific journal
- Elsevier BV, Nov. 2018, International Journal of Infectious Diseases, 76, 130 - 136, English[Refereed]Scientific journal
- Nov. 2018, Journal of Japan Society of Civil Engineers (B2), 74(2) (2), I_1225 - I_1230, Japanese[Refereed]Scientific journal
- Nov. 2018, Journal of Japan Society of Civil Engineers (B2), 74(2) (2), I_1231 - I_1236, Japanese[Refereed]Scientific journal
- Last, University of Chicago Press, Jun. 2018, Freshwater Science, 37(2) (2), 307 - 314, English[Refereed]Scientific journal
- We present a performance evaluation of environmental DNA (eDNA) metabarcoding with MiFish-U/E primers to investigate local and regional diversities of stream fish species to examine potential effectiveness, limits and future remedies of this technique in large-scale monitoring. We hypothesised that eDNA inferences are more consistent with fish assemblages observed upstream than downstream due to a directional flow of river water. River water was sampled at 102 sites in 51 rivers around Lake Biwa in the central part of Honshu Island, Japan, within 10 person-days, and fish species compositions inferred from eDNA and existing observational data were compared. Observation sites were chosen from the observational data that were within a certain distance (buffer range) of a water-sampling site along a river trajectory. The hypothesis of the detection bias of eDNA towards upstream assemblage was tested by comparing results with all of the observational data, data from a higher elevation and data from a lower elevation. The Jaccard dissimilarity index was plotted between the observational data and the eDNA estimates against the buffer range the buffer range with minimum dissimilarity was chosen. When using existing observational data from within 6 km upstream of the eDNA sampling sites, the eDNA results were the most consistent with the observational data and inferred 86.4% of the species reported (38/44), as well as two additional species. eDNA results also showed patterns consistent with known upstream–downstream turnover of related species and biogeographical assemblage patterns of certain species. Our 10-person-days survey using the metabarcoding technique enabled us to obtain as much regional fish diversity data including the hypothesised pattern of eDNA detection with an upstream bias as the accumulated observational data obtained through greater amounts of time, money and labour. The problems regarding false-positive/negative detection were suggested in our survey however, these should be decreased or removed by modifying the sampling methods and experimental procedures in future works. Therefore, we concluded this new tool to enable monitoring that has never been implemented, such as cross-nation, and even whole-Earth monitoring with the data at yearly, seasonal or finer temporal scales.Blackwell Publishing Ltd, Jun. 2018, Freshwater Biology, 63(6) (6), 569 - 580, English[Refereed]Scientific journal
- Mar. 2018, Metabarcoding & Metagenomics, 2018, e23297, English[Refereed]Scientific journal
- Environmental DNA (eDNA) analysis for macro-organisms has developed rapidly, and studies have reported the application of such analyses to various kinds of organism. However, only a few studies have compared the results of eDNA analysis and traditional sampling surveys on a fine scale, and the usefulness of eDNA analysis for understanding the distribution of endangered species on a finer scale has not been sufficiently assessed. In this study, we conducted eDNA analysis and a traditional sampling survey in multiple 500-m sections of a river and compared the results. The survey targets were the endangered fish species Liobagrus reinii, Coreoperca kawamebari, and Lethenteron sp. 2, which are known to occur in the surveyed river. We established 23, 500-m-long sections in the Sasayama River and sampled water at the downstream end of each section. The eDNA of the target species was detected using real-time PCR assays developed in this study. We also collected the target species in traditional sampling surveys and recorded the presence/absence of the target species in each section. The rates of concordance between the results of the eDNA analysis and the traditional sampling surveys varied among the species. Our result showed that the results of eDNA analysis and field sampling surveys do not necessarily match at a finer scale, and suggested the importance of knowledge accumulation for eDNA analysis for proper use of this analysis method to conserve endangered species.The Ecological Society of Japan, 2018, Japanese Journal of Conservation Ecology, 23(2) (2), 257 - 264, Japanese[Refereed]Scientific journal
- This study investigated the effect of valuators' personal history and beliefs on valuation of ecosystem services around Mt. Rokko, which is located near Kobe, a major city in Japan. Special attention was paid to how differences in lifestyle, access to nature, and experiences during childhood influence willingness to pay (WTP) for peri-urban ecosystem conservation. The estimated values (median: 1858 JPY) were relatively higher than in previous case studies including one on the World Heritage forest in the country, but the value was not outlier of the literature. From the simple model estimation, we focused on the effect of individual differences. The full model including information of personal experience with nature revealed differences in WTP among residents. The basic characteristics of age and income were found to have a significant effect. Interestingly, it was also found that certain experiences during childhood had a significant effect on increasing WTP for ecosystem and biodiversity conservation. These findings suggest the importance of considering the diversity of valuators in ecosystem valuation studies under urbanization processes, and it also warns the extinction of experience with nature under on going urbanization for urban ecosystem conservation.ELSEVIER GMBH, URBAN & FISCHER VERLAG, Dec. 2017, URBAN FORESTRY & URBAN GREENING, 28, 110 - 117, English[Refereed]Scientific journal
- Freshwater biodiversity has been severely threatened in recent years, and to conserve endangered species, their distribution and breeding habitats need to be clarified. However, identifying breeding sites in a large area is generally difficult. Here, by combining the emerging environmental DNA (eDNA) analysis with subsequent traditional collection surveys, we successfully identified a breeding habitat for the critically endangered freshwater fish Acheilognathus typus in the mainstream of Omono River in Akita Prefecture, Japan, which is one of the original habitats of this species. Based on DNA cytochrome B sequences of A. typus and closely related species, we developed species-specific primers and a probe that were used in real-time PCR for detecting A. typus eDNA. After verifying the specificity and applicability of the primers and probe on water samples from known artificial habitats, eDNA analysis was applied to water samples collected at 99 sites along Omono River. Two of the samples were positive for A. typus eDNA, and thus, small fixed nets and bottle traps were set out to capture adult fish and verify egg deposition in bivalves (the preferred breeding substrate for A. typus) in the corresponding regions. Mature female and male individuals and bivalves containing laid eggs were collected at one of the eDNA-positive sites. This was the first record of adult A. typus in Omono River in 11 years. This study highlights the value of eDNA analysis to guide conventional monitoring surveys and shows that combining both methods can provide important information on breeding sites that is essential for species' conservation.SPRINGER HEIDELBERG, Dec. 2017, SCIENCE OF NATURE, 104(11-12) (11-12), 100, English[Refereed]Scientific journal
- The advent of environmental DNA (eDNA) analysis methods has enabled rapid and wide-range ecological monitoring in aquatic ecosystems, but there is a dearth of information on eDNA degradation. The results of previous studies suggest that the decay rate of eDNA varies depending on the length of DNA fragments. To examine this hypothesis, we compared temporal change in copy number of long eDNA fragments (719bp) with that of short eDNA fragments (127bp). First, we isolated rearing water from a target fish species, Japanese Jack Mackerel (Trachurus japonicus), and then quantified the copy number of the long and short eDNA fragments in 1L water samples after isolating the water from the fish. Long DNA fragments showed a higher decay rate than short fragments. Next, we measured the eDNA copy numbers of long and short DNA fragments using field samples, and compared them with fish biomass as measured by echo intensity. Although a previous study suggested that short eDNA fragments could be overestimated because of nontarget eDNA from a nearby fish market and carcasses, the eDNA concentrations of long fragments were correlated with echo intensity. This suggests that the concentration of longer eDNA fragments reflects fish biomass more accurately than the previous study by removing the effects of the fish market and carcasses. The length-related differences in eDNA have a substantial potential to improve estimation of species biomass.WILEY, Nov. 2017, MOLECULAR ECOLOGY RESOURCES, 17(6) (6), e25 - e33, English[Refereed]Scientific journal
- Environmental DNA (eDNA) techniques utilizing DNA fragments from water have recently been developed to investigate the distribution and abundance/biomass of aquatic organisms. The eDNA technique is based on the analysis of DNA fragments in sampled water; thus, an unmanned aerial vehicle (UAV; drone) would be a useful way of collecting water for eDNA sampling, and may consequently allow us to extend eDNA surveys both spatially and temporally. Here, we developed a new method of water collection by using UAV with bleachable equipment, to avoid DNA contamination. To test the performance and contamination risk of UAV water sampling in eDNA surveys, we sampled water from a dam reservoir, detected eDNA from two fish species, and compared the water samples obtained by UAV with those obtained by boat. Additionally, we investigated contamination using blank samples. The results revealed that our UAV water sampling method performed similar to the boat sampling method. No positive signals were detected in the blank samples, including those used for UAV sampling, transportation, filtering, and PCR blanks. Our UAV method can be used to investigate species distributions using eDNA. Combinations of UAV technologies, including remote and thermal sensing, will enable efficient environmental monitoring in various waterbodies.WILEY, Nov. 2017, LIMNOLOGY AND OCEANOGRAPHY-METHODS, 15(11) (11), 939 - 944, English[Refereed]Scientific journal
- Understanding behavioral differences between intraspecific genotypes of aquatic animals is challenging because we cannot directly observe the animals underwater or visually distinguish morphologically similar counterparts. Here, we tested a new monitoring tool that uses environmental DNA (eDNA), an assemblage of DNA in environmental water, to specifically detect Japanese native and introduced non-native genotypes of common carp (Cyprinus carpio) in Lake Biwa, Japan, and estimated differences between the two genotypes in the use of inland habitats. We monitored the ratios of native and non-native single nucleotide polymorphism alleles of a mitochondrial locus of common carp in a lagoon connected to Lake Biwa for 3years using eDNA. We observed seasonal dynamics in the allele frequency showing that the native genotype frequency peaked every spring, suggesting that native individuals migrated to the lagoon for spawning and then returned to the main lake, whereas non-native individuals tended to stay in the lagoon. The estimated migration patterns corresponded with the estimates of a previous study, which were based on commercial fish catch data. Our findings suggest that eDNA-based monitoring can be useful tool for addressing intraspecific behavioral differences underwater.WILEY, Oct. 2017, ECOLOGY AND EVOLUTION, 7(20) (20), 8515 - 8522, English[Refereed]Scientific journal
- Public Library of Science (PLoS), May 2017, PLOS ONE, 12(5) (5), e0176541 - e0176541, English[Refereed]Scientific journal
- Opisthorchiasis, which can lead to cholangiocarcinoma in cases of chronic infection, is a major public health problem in Southeast Asian countries. The trematode, Opisthorchis viverrini, is the causative agent of the disease. Accurate and rapid monitoring of O. viverrini is crucial for disease prevention and containment. Therefore, in this study we sought to develop a novel species -specific real-time PCR assay for detecting O. viverrini using environmental DNA (eDNA). The diagnostic sensitivity of the newly developed real-time PCR assay was similar to that of the traditional PCR assay for 50 fecal samples collected in Lao PDR (21 and 19 samples were positive by real-time PCR and traditional PCR, respectively). The efficacy of eDNA analysis and its applicability in the field were tested using a total of 94 environmental water samples collected from 44 sites in Savannakhet, Lao PDR during May and October 2015 and February 2016. O. viverrini eDNA was detected in five samples by real-time PCR, indicating the presence of the fluke in the area and the risk of infection for individuals consuming fish from these water sources. The application of eDNA analysis would facilitate the identification of O. viverrini endemic hotspots and contribute to the ecological control of opisthorchiasis, and this strategy can be applied to other eukaryotic water pathogens. (C)2017 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, May 2017, ACTA TROPICA, 169, 1 - 7, English[Refereed]Scientific journal
- Environmental DNA (eDNA) is DNA shed by organisms into surrounding environments such as soil and water. The new methods using eDNA as a marker for species detection are being rapidly developed. Here we explore basic knowledge regarding the dependence of the eDNA degradation rate on time and water temperature, and the relationship between eDNA degradation and bacterial abundance. This subject has not been well clarified, even though it is essential for improving the reliability of eDNA analysis. To determine the time-and water temperature-dependent degradation of eDNA, river water was sampled and eDNA concentrations were determined for ayu sweetfish (Plecoglossus altivelis altivelis) and common carp (Cyprinus carpio) at seven time points, over a 48-h period, and at three different water temperatures. The degradation of eDNA was modeled for each species using an existing exponential decay model with an extension to include water temperature effects. The degradation models were constructed for ayu sweetfish as N-t = 229,901.2 x exp [- (0.01062 x k - 0.07081) x t] and for common carp as N-t = 2,558.0 x exp [- (0.01075 x k - 0.07372) x t]. Nt is the DNA concentration at time t (elapsed time in hours) and k is the water temperature (degrees C). We also measured the concentration of eDNA derived from purified genomic DNA of the common carp, which was spiked into aquarium water without the target species, and we measured the bacterial abundance in the sample water after 12 and 24 h of incubation. Environmental DNA degradation was accelerated at higher water temperatures (generalized linear model, GLM; p < 0.001), but bacterial abundance did not have a significant effect on eDNA degradation (GLM, p = 0.097). These results suggest that the proper treatment of this temperature effect in data interpretations and adjustments would increase the reliability of eDNA analysis in future studies.PUBLIC LIBRARY SCIENCE, Apr. 2017, PLOS ONE, 12(4) (4), e0176608, English[Refereed]Scientific journal
- Environmental DNA (eDNA) analysis is a powerful tool within ecology for the study of the distribution or abundance of aquatic species, although the simplification of water sampling is required for enabling light and fast field sampling to expand further application of eDNA analysis. Here, certain candidate chemicals belonging to the group of cationic surfactants were examined for their effectiveness as preservatives for eDNA water samples by simply adding the chemicals to water samples to suppress the degradation of eDNA. The quaternary ammonium compound benzalkonium chloride (BAC) at a final concentration of 0.01% was effective to retain 92% of eDNA derived from the bluegill sunfish Lepomis macrochirus in an 8-h incubation test at ambient temperature, which assumed a transportation of water samples in 1-day field sampling during the daytime. Meanwhile, eDNA in water samples without BAC retained only 14% of the initial eDNA. Moreover, an additional long-term incubation test (up to 10 days) revealed BAC-treated samples retained similar to 70 and 50% of bluegill DNA compared to the initial amount after 1- and 10-day incubation at ambient temperature, respectively. Meanwhile, eDNA in naive samples reduced to 20% after 1-day incubation and reached undetectable levels after 10 days. Up to now, many eDNA studies have adopted on-site filtration followed by filter fixation, which requires many pieces of equipment. Addition of BAC can protect eDNA in water samples with less effort and equipment resulting in an increase of measurement accuracy of the eDNA quantity and detection probability of rare species by preventing the disappearance of rare sequences in water samples.SPRINGER JAPAN KK, Apr. 2017, LIMNOLOGY, 18(2) (2), 233 - 241, English[Refereed]Scientific journal
- The recently developed environmental DNA (eDNA) analysis has been used to estimate the distribution of aquatic vertebrates by using mitochondrial DNA (mtDNA) as a genetic marker. However, mtDNA markers have certain drawbacks such as variable copy number and maternal inheritance. In this study, we investigated the potential of using nuclear DNA (ncDNA) as a more reliable genetic marker for eDNA analysis by using common carp (Cyprinus carpio). We measured the copy numbers of cytochrome b (CytB) gene region of mtDNA and internal transcribed spacer 1 (ITS1) region of ribosomal DNA of ncDNA in various carp tissues and then compared the detectability of these markers in eDNA samples. In the DNA extracted from the brain and gill tissues and intestinal contents, CytB was detected at 95.1 +/- 10.7 (mean +/- 1 standard error), 29.7 +/- 1.59 and 24.0 +/- 4.33 copies per cell, respectively, and ITS1 was detected at 1760 +/- 343, 2880 +/- 503 and 1910 +/- 352 copies per cell, respectively. In the eDNA samples from mesocosm, pond and lake water, the copy numbers of ITS1 were about 160, 300 and 150 times higher than those of CytB, respectively. The minimum volume of pond water required for quantification was 33 and 100 mL for ITS1 and CytB, respectively. These results suggested that ITS1 is a more sensitive genetic marker for eDNA studies of C. carpio.WILEY, Mar. 2017, MOLECULAR ECOLOGY RESOURCES, 17(2) (2), 324 - 333, English[Refereed]Scientific journal
- The environmental DNA (eDNA) method has been used to estimate the distributions of aquatic species, and both ethanol precipitation and filtration-based methods are commonly employed to capture eDNA from sampled water. Although filtration-based methods can capture more eDNA than that by ethanol precipitation, by processing larger volumes of water (e.g., 1 L vs. 15 mL), ethanol precipitation can immediately preserve eDNA on site and ease downstream processing, which are especially advantageous for eDNA studies conducted with limited resources, such as electric equipment, labor, and time. However, the ethanol precipitation method is limited by small volume of water that can be processed (i.e., 15 mL in a reaction volume of 50 mL). As an alternative, isopropanol could potentially be used to increase the volume of sample water processed, since lower volumes of isopropanol are required for precipitation. Therefore, in the present study, we compared the copy numbers of carp eDNA captured using isopropanol and ethanol precipitation in both mesocosm and field experiments. In both cases, we found that isopropanol precipitation recovered double the amount of eDNA recovered by ethanol precipitation, when the reaction volumes were equal. Therefore, isopropanol precipitation is a superior option for eDNA capture when surveys are conducted under resource-limited conditions.WILEY, Feb. 2017, LIMNOLOGY AND OCEANOGRAPHY-METHODS, 15(2) (2), 212 - 218, English[Refereed]Scientific journal
- Recent development of environmental DNA (eDNA) analysis allows us to survey underwater macro-organisms easily and cost effectively; however, there have been no reports on eDNA detection or quantification for jellyfish. Here we present the first report on an eDNA analysis of marine jellyfish using Japanese sea nettle (Chrysaora pacifica) as a model species by combining a tank experiment with spatial and temporal distribution surveys. We performed a tank experiment monitoring eDNA concentrations over a range of time intervals after the introduction of jellyfish, and quantified the eDNA concentrations by quantitative real-time PCR. The eDNA concentrations peaked twice, at 1 and 8 h after the beginning of the experiment, and became stable within 48 h. The estimated release rates of the eDNA in jellyfish were higher than the rates previously reported in fishes. A spatial survey was conducted in June 2014 in Maizuru Bay, Kyoto, in which eDNA was collected from surface water and sea floor water samples at 47 sites while jellyfish near surface water were counted on board by eye. The distribution of eDNA in the bay corresponded with the distribution of jellyfish inferred by visual observation, and the eDNA concentration in the bay was similar to 13 times higher on the sea floor than on the surface. The temporal survey was conducted from March to November 2014, in which jellyfish were counted by eye every morning while eDNA was collected from surface and sea floor water at three sampling points along a pier once a month. The temporal fluctuation pattern of the eDNA concentrations and the numbers of observed individuals were well correlated. We conclude that an eDNA approach is applicable for jellyfish species in the ocean.PUBLIC LIBRARY SCIENCE, Feb. 2017, PLOS ONE, 12(2) (2), e0173073, English[Refereed]Scientific journal
- Heavy water is a form of water that contains a heavier isotope of hydrogen (2 H, also known as deuterium, D) or oxygen (17O or18O). When using heavy water as a solvent, error-prone polymerase chain reaction (epPCR) can induce random mutations independent of the polymerase used or the composition of the PCR reaction mixture. This relatively new method can easily be combined with the existing epPCR methods to increase the rate of mutations.Humana Press Inc., 2017, Methods in Molecular Biology, 1498, 491 - 495, English[Refereed]International conference proceedings
- Environmental DNA (eDNA) metabarcoding has emerged as a potentially powerful tool to assess aquatic community structures. However, the method has hitherto lacked field tests that evaluate its effectiveness and practical properties as a biodiversity monitoring tool. Here, we evaluated the ability of eDNA metabarcoding to reveal fish community structures in species-rich coastal waters. High-performance fish-universal primers and systematic spatial water sampling at 47 stations covering similar to 11 km(2) revealed the fish community structure at a species resolution. The eDNA metabarcoding based on a 6-h collection of water samples detected 128 fish species, of which 62.5% (40 species) were also observed by underwater visual censuses conducted over a 14-year period. This method also detected other local fishes (>= 23 species) that were not observed by the visual censuses. These eDNA metabarcoding features will enhance marine ecosystem-related research, and the method will potentially become a standard tool for surveying fish communities.NATURE PUBLISHING GROUP, Jan. 2017, SCIENTIFIC REPORTS, 7, 40368, English[Refereed]Scientific journal
- 1. Environmental DNA (eDNA) analysis for detecting the presence of aquatic and terrestrial organisms is an established method, and the eDNA concentration of a species can reflect its abundance/biomass at a site. However, attempts to estimate the abundance/biomass of aquatic species using eDNA concentrations in large stream and river ecosystems have received little attention. 2. We determined the eDNA concentration and abundance/biomass of a stream fish, Plecoglossus altivelis, by conducting a snorkelling survey in the Saba River, Japan. Furthermore, we evaluated the relationship between eDNA concentrations and the estimated abundance/biomass of P. altivelis, and determined its spatial distribution within the river. 3. Across the three seasons from spring to autumn, we found significant correlations between the eDNA concentration of P. altivelis and its abundance/biomass at study sites within the river. We detected the eDNA at the sites where we found only feeding traces on stones (where P. altivelis was not directly observed), but not at sites without feeding traces. Additionally, we tested the optimal number of qPCR replicates needed for the eDNA evaluation of P. altivelis abundance and biomass; only a small number of replicates was required when the eDNA concentration was high. 4. Our findings suggest that eDNA analysis is a useful tool to estimate fish abundance/biomass as well as their spatial distribution in rivers.WILEY-BLACKWELL, Jan. 2017, FRESHWATER BIOLOGY, 62(1) (1), 30 - 39, English[Refereed]Scientific journal
- Environmental DNA (eDNA) analysis has recently been applied to the study of aquatic macroorganisms. In most studies, sample water was filtered and the extracted DNA from the residues on the filter used for the following molecular analysis to detect species of interest. This quick, new biomonitoring method has received broad attention, but some unknowns remain, such as the eDNA yield in relation to water quality. Previous studies suggest that eDNA is composed of various forms, such as the free-floating naked form and in organelles and cells. Therefore, the eDNA yield in the filtration and extraction steps might change depending on the composition of eDNA. Especially the filtration efficiency of free-floating DNA would be affected by the electrical effect of water pH. In this study, not only the free-floating naked DNA, but also all DNA fragments released from the organisms and contained in the water were defined as eDNA, including cells and organelles. We examined (1) the effect of water pH on the eDNA yield at filtration and (2) the effect of proteinase K treatment on the extraction efficiency of DNA from filter samples, with consideration of the variety of the eDNA forms in water. In a laboratory experiment using the purified DNA of common carp (Cyprinus carpio carpio) spiked into ultrapure water, the water pH and DNA yield showed a negative relationship within the pH range of 5-9, that is, the DNA yield was higher in acidic conditions, plausibly because of pH-dependent adsorption onto the glass fiber filter at the filtration step. In case the field water contained eDNA derived from the inhabiting common carp and the purified DNA of ayu (Plecoglossus altivelis altivelis) spiked in the sample as an internal standard, adjustment of the pH to 5 prior to filtration did not increase the eDNA yield of common carp, and the spiked ayu DNA was not detected at all. During the DNA extraction step, a standard protocol including proteinase K treatment marked higher DNA yield than that without proteinase K treatment. Overall, the present results indicate successful collection of eDNA using filters without any special attention to the pH of the sample water, and a conventional protocol with proteinase K treatment is appropriate for eDNA recovery.SPRINGER JAPAN KK, Jan. 2017, LIMNOLOGY, 18(1) (1), 1 - 7, English[Refereed]Scientific journal
- Recent studies demonstrated the use of environmental DNA (eDNA) from fishes to be appropriate as a non-invasive monitoring tool. Most of these studies employed disk fiber filters to collect eDNA from water samples, although a number of microbial studies in aquatic environments have employed filter cartridges, because the cartridge has the advantage of accommodating large water volumes and of overall ease of use. Here we provide a protocol for filtration of water samples using the filter cartridge and extraction of eDNA from the filter without having to cut open the housing. The main portions of this protocol consists of 1) filtration of water samples (water volumes <= 4 L or > 4 L); (2) extraction of DNA on the filter using a roller shaker placed in a preheated incubator; and (3) purification of DNA using a commercial kit. With the use of this and previously-used protocols, we perform metabarcoding analysis of eDNA taken from a huge aquarium tank (7,500 m(3)) with known species composition, and show the number of detected species per library from the two protocols as the representative results. This protocol has been developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.JOURNAL OF VISUALIZED EXPERIMENTS, Nov. 2016, JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 117(117) (117), e54741, English[Refereed][Invited]Scientific journal
- Environmental DNA (eDNA) analysis is an innovative tool for determining the distribution or abundance of aquatic macroorganisms. However, because eDNA degrades rapidly in water, long delays between sampling and analysis may hinder eDNA quantification. In the present study, we developed a portable filtration system that enables on-site (and on-the-road) filtration of water samples. Degradation rates of eDNA within 6 h were compared using water from an outdoor pond that was subjected to (1) on-site filtration, (2) transportation of water on ice, and (3) transportation of water at ambient temperature. Groups 2 and 3 were filtered in the laboratory 6 h after sampling. The concentration of eDNA was determined as the copy number of the mitochondrial cytochrome b gene of two fish species using real-time polymerase chain reaction. The portable filtration system offers the following benefits: (1) the eDNA concentration is preserved as is at the time of sampling, permitting higher accuracy of eDNA quantification, (2) use of a disposable sealed plastic bag reduces the risk of contamination and ensures on-the-road filtration, (3) time is saved because filtration can be accomplished when driving between sampling sites.SPRINGER JAPAN KK, Nov. 2016, ECOLOGICAL RESEARCH, 31(6) (6), 963 - 967, English[Refereed]Scientific journal
- The first step toward solving the problems caused by an invasive alien species is to know the distribution of the species. However, species in underwater environments are difficult to investigate. The recent development of environmental DNA (eDNA) analysis has made it possible to investigate the distribution of a target species simply by analyzing the DNA in the water. To date, few investigators have used eDNA detection of aquatic plants. We established an eDNA detection method for Egeria densa, an invasive aquatic plant species in Japan; used eDNA detection to survey the species in aquaria; and applied this method to water samples from 23 outdoor ponds. We also used visual observations of the ponds. The aquarium experiments revealed that the eDNA concentration in the water increased rapidly and peaked 1 or 2 d after starting the experiment, after which it decreased rapidly, reaching its lowest point on the 5th day. In the field surveys, we visually observed E. densa at 5 ponds, and the eDNA of E. densa was detected from the same 5 ponds. Thus, the eDNA results perfectly matched the observational results. Our work confirms that detection of aquatic plants by eDNA analysis is feasible.UNIV CHICAGO PRESS, Jun. 2016, FRESHWATER SCIENCE, 35(2) (2), 748 - 754, English[Refereed]Scientific journal
- The environmental DNA (eDNA) method has increasingly been recognized as a powerful tool for monitoring aquatic animal species; however, its application for monitoring aquatic plants is limited. To evaluate eDNA analysis for estimating the distribution of aquatic plants, we compared its estimated distributions with eDNA analysis, visual observation, and past distribution records for the submerged species Hydrilla verticillata. Moreover, we conducted aquarium experiments using H. verticillata and Egeria densa and analyzed the relationships between eDNA concentrations and plant biomass to investigate the potential for biomass estimation. The occurrences estimated by eDNA analysis closely corresponded to past distribution records, and eDNA detections were more frequent than visual observations, indicating that the method is potentially more sensitive. The results of the aquarium experiments showed a positive relationship between plant biomass and eDNA concentration; however, the relationship was not always significant. The eDNA concentration peaked within three days of the start of the experiment in most cases, suggesting that plants do not release constant amounts of DNA. These results showed that eDNA analysis can be used for distribution surveys, and has the potential to estimate the biomass of aquatic plants.PUBLIC LIBRARY SCIENCE, Jun. 2016, PLOS ONE, 11(6) (6), English[Refereed]Scientific journal
- Supporting citizens' activity scrutinizing scientific information: citizens' seminar on IPCC reports平成27年度日本科学教育学会第7回研究会（四国支部開催); [日時] 平成28年5月28日（土）; [会場]香川大学; [主催]一般社団法人日本科学教育学会Japanese Society for Science Education, May 2016, 日本科学教育学会研究会研究報告, 30(7) (7), 17 - 20, JapaneseSymposium
- Supporting research activities of high school students using environmental DNA analysis平成27年度日本科学教育学会第7回研究会（四国支部開催); [日時] 平成28年5月28日（土）; [会場]香川大学; [主催]一般社団法人日本科学教育学会Japanese Society for Science Education, May 2016, 日本科学教育学会研究会研究報告, 30(2) (2), 21 - 24, JapaneseSymposium
- The invasion of non-native species that are closely related to native species can lead to competitive elimination of the native species and/or genomic extinction through hybridization. Such invasions often become serious before they are detected, posing unprecedented threats to biodiversity. A Japanese native strain of common carp (Cyprinus carpio) has become endangered owing to the invasion of non-native strains introduced from the Eurasian continent. Here, we propose a rapid environmental DNA-based approach to quantitatively monitor the invasion of non-native genotypes. Using this system, we developed a method to quantify the relative proportion of native and non-native DNA based on a single-nucleotide polymorphism using cycling probe technology in real-time PCR. The efficiency of this method was confirmed in aquarium experiments, where the quantified proportion of native and non-native DNA in the water was well correlated to the biomass ratio of native and non-native genotypes. This method provided quantitative estimates for the proportion of native and non-native DNA in natural rivers and reservoirs, which allowed us to estimate the degree of invasion of non-native genotypes without catching and analysing individual fish. Our approach would dramatically facilitate the process of quantitatively monitoring the invasion of non-native conspecifics in aquatic ecosystems, thus revealing a promising method for risk assessment and management in biodiversity conservation.WILEY-BLACKWELL, Mar. 2016, MOLECULAR ECOLOGY RESOURCES, 16(2) (2), 415 - 422, English[Refereed]Scientific journal
- Recent studies in streams and ponds have demonstrated that the distribution and biomass of aquatic organisms can be estimated by detection and quantification of environmental DNA (eDNA). In more open systems such as seas, it is not evident whether eDNA can represent the distribution and biomass of aquatic organisms because various environmental factors (e.g., water flow) are expected to affect eDNA distribution and concentration. To test the relationships between the distribution of fish and eDNA, we conducted a grid survey in Maizuru Bay, Sea of Japan, and sampled surface and bottom waters while monitoring biomass of the Japanese jack mackerel (Trachurus japonicus) using echo sounder technology. A linear model showed a high R-2 value (0.665) without outlier data points, and the association between estimated eDNA concentrations from the surface water samples and echo intensity was significantly positive, suggesting that the estimated spatial variation in eDNA concentration can reflect the local biomass of the jack mackerel. We also found that a best-fit model included echo intensity obtained within 10-150 m from water sampling sites, indicating that the estimated eDNA concentration most likely reflects fish biomass within 150 m in the bay. Although eDNA from a wholesale fish market partially affected eDNA concentration, we conclude that eDNA generally provides a 'snapshot' of fish distribution and biomass in a large area. Further studies in which dynamics of eDNA under field conditions (e.g., patterns of release, degradation, and diffusion of eDNA) are taken into account will provide a better estimate of fish distribution and biomass based on eDNA.PUBLIC LIBRARY SCIENCE, Mar. 2016, PLOS ONE, 11(3) (3), e1249786, English[Refereed]Scientific journal
- This study demonstrated the use of environmental DNA (eDNA) to determine habitat connectivity for migration of fishes between the sea and river. Environmental DNA is DNA fragments released by fishes in water, which can be used as a species-specific marker of the presence/absence of the target species. A year-round water sampling regime at 15 sites on the Yodo River, Japan, was conducted to determine whether three major man-made barriers on the river inhibited the migration of fishes using species-specific detection of DNA fragments from three target migrant species, temperate seabass, Lateolabrax japonicas, flathead grey mullet, Mugil cephalus, and ayu, Plecoglossus altivelis altivelis. The presence/absence of eDNA from target species was consistent with known patterns of species' seasonal migration. The detection of the DNA of temperate seabass and flathead grey mullet at sites upstream of the dam closest to the river mouth indicated successful upstream migration of these species via a fish ladder bypassing the dam. On the other hand, DNA of these two species was not detected from the upstream side of the two remaining dams, which are not equipped with fish ladders. Ayu is the only species among the three target species with a land-locked population in Lake Biwa located at the headwater of Yodo River. Ayu DNA was detected at most of the sites in the freshwater area during the warm months; however, in the coldest month of February, eDNA was only detected in the uppermost site of Yodo River at the southern tip of Lake Biwa. The eDNA we detected at this site suggests that it was derived from juvenile ayu spending their winter months in the lake. These results suggest that the eDNA analysis presented here can accurately track the seasonal migration of fishes in a river, demonstrating its application as an indicator of habitat connectivity for fishes in association with man-made barriers in a river. The sampling of eDNA involves merely scooping a tank full of water; therefore, it is a simple, rapid, and cost-effective method for long-term monitoring of habitat connectivity associated with the construction of barriers in a river. (C) 2015 Elsevier Ltd. All rights reserved.ELSEVIER SCIENCE BV, Mar. 2016, ECOLOGICAL INDICATORS, 62, 147 - 153, English[Refereed]Scientific journal
- Environmental DNA (eDNA) analysis has recently been used for detection of aquatic macro-organisms; however, the analytical procedures used in previous studies have not been optimized for practical use. Here, we compared several methods for DNA enrichment and extraction from water samples to establish widely applicable techniques for eDNA analysis using common carp as the model species. First, several types of filters were compared to identify the optimal filter type. Second, the eDNA yield was compared after a variety of extraction and isolation steps, including a combination of phenol extraction, ethanol precipitation (phenol treatment), and ultrafiltration. Third, DNA fixation with ethanol was tested for the preservation of eDNA on filters. Ethanol precipitation yielded the largest number of eDNA copies, followed by filtering using a 0.2-mu m polycarbonate filter and a 0.7-mu m glass fiber filter. Phenol treatment resulted in collection of a higher number of eDNA copies than that collected using ultrafiltration. DNA fixation with 15 ml ethanol enabled eDNA preservation on the filters at ambient temperatures for at least 6 days. Finally, combinations of different filter types and DNA enrichment procedures were compared using field water samples. From these results, we propose that the appropriate selection method for eDNA analysis should be chosen based on context. For example, when a high concentration of the target DNA is expected, such as in an aquarium experiment, ethanol precipitation is advantageous. However, when the target DNA is rare, which is the case in most field studies, filtration followed by freezing or DNA fixation by ethanol and phenol treatment are recommended. The filter type should be decided prior to the survey based on the characteristics of the water of interest. Thus, eDNA analysis could be applied to various situations using adaptive combinations of these techniques.SPRINGER JAPAN KK, Jan. 2016, LIMNOLOGY, 17(1) (1), 23 - 32, English[Refereed]Scientific journal
- Distribution survey methods of macro-organisms using environmental DNA（eDNA）have been rapidly developing in recent years. Environmental DNA analysis can be divided into two major methods, species-specific detection and eDNA metabarcoding. Here we outline these techniques, and we report the preliminary results of an eDNA-based biomass survey of Japanese jack mackere（l Trachurus japonicus）. The results showed significantly positive associations between eDNA concentrations from water samples and echo intensity detected by an echo sounder, suggesting that the spatial variation of the eDNA concentration can reflect the local biomass of fish even in marine environments. More detailed distribut ion mapping of coastal fish can be achieved by environmental DNA analysis, and it will contribute in various fields, such as fisheries science, ichthyology, and ecology.Coastal Oceanography Research Committee, the Oceanographic Society of Japan, 2016, Bulletin on Coastal Oceanography, 53(2) (2), 173 - 178, Japanese[Refereed][Invited]Scientific journal
- In recent years, the degradation of biodiversity has advanced significantly, especially in freshwater ecosystems. To conserve rare species, the distribution of the target species should be known, even if the density is very low. Traditional habitat surveys using direct catches or observations require much time, labor, and expertise. Over the last decade, environmental DNA (eDNA) analysis methods that complement traditional surveys have been developed. In the present study, we developed an eDNAbased detection method for a Cyprinidae species, Hemigrammocypris rasborella, and applied it in natural habitats. First, we tested our method in 11 irrigation ponds for which information on the distribution of H. rasborella was available. The eDNA detection results matched completely with the known presence/absence data. Next, we applied this method to 81 irrigation ponds for which no distribution information was available, and detected the eDNA of H. rasborella in 6 ponds. Subsequently, we conducted capture surveys in the 6 eDNA-positive ponds and found the species in 5 ponds. These results suggest that eDNA analysis is useful for the monitoring of rare species.Tohoku University, 2016, Japanese Journal of Ecology, 66(3) (3), 613 - 620, Japanese[Refereed]Scientific journal
- Tohoku University, 2016, Japanese Journal of Ecology, 66(3) (3), 601 - 612, Japanese[Refereed]Scientific journal
- Tohoku University, 2016, Japanese Journal of Ecology, 66(3) (3), 583 - 600, Japanese[Refereed]Scientific journal
- Springer Science and Business Media LLC, Dec. 2015, Artificial Life and Robotics, 20(4) (4), 347 - 352, English[Refereed]Scientific journal
- We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding environmental DNA (eDNA) from fishes. Primers were designed using aligned whole mitochondrial genome (mitogenome) sequences from 880 species, supplemented by partial mitogenome sequences from 160 elasmobranchs (sharks and rays). The primers target a hypervariable region of the 12S rRNA gene (163-185 bp), which contains sufficient information to identify fishes to taxonomic family, genus and species except for some closely related congeners. To test versatility of the primers across a diverse range of fishes, we sampled eDNA from four tanks in the Okinawa Churaumi Aquarium with known species compositions, prepared dual-indexed libraries and performed paired-end sequencing of the region using high-throughput next-generation sequencing technologies. Out of the 180 marine fish species contained in the four tanks with reference sequences in a custom database, we detected 168 species (93.3%) distributed across 59 families and 123 genera. These fishes are not only taxonomically diverse, ranging from sharks and rays to higher teleosts, but are also greatly varied in their ecology, including both pelagic and benthic species living in shallow coastal to deep waters. We also sampled natural seawaters around coral reefs near the aquarium and detected 93 fish species using this approach. Of the 93 species, 64 were not detected in the four aquarium tanks, rendering the total number of species detected to 232 (from 70 families and 152 genera). The metabarcoding approach presented here is non-invasive, more efficient, more cost-effective and more sensitive than the traditional survey methods. It has the potential to serve as an alternative (or complementary) tool for biodiversity monitoring that revolutionizes natural resource management and ecological studies of fish communities on larger spatial and temporal scales.ROYAL SOC, Jul. 2015, ROYAL SOCIETY OPEN SCIENCE, 2(7) (7), 150088, English[Refereed]Scientific journal
- The Japanese Society of Irrigation, Drainage and Rural Engineering, Jun. 2015, Irrigation, Drainage and Rural Engineering Journal, 297(83-3) (83-3), IV_7 - IV_8, English[Refereed]
- Environmental DNA (eDNA) has been used to investigate species distributions in aquatic ecosystems. Most of these studies use real-time polymerase chain reaction (PCR) to detect eDNA in water; however, PCR amplification is often inhibited by the presence of organic and inorganic matter. In droplet digital PCR (ddPCR), the sample is partitioned into thousands of nanoliter droplets, and PCR inhibition may be reduced by the detection of the end-point of PCR amplification in each droplet, independent of the amplification efficiency. In addition, real-time PCR reagents can affect PCR amplification and consequently alter detection rates. We compared the effectiveness of ddPCR and real-time PCR using two different PCR reagents for the detection of the eDNA from invasive bluegill sunfish, Lepomis macrochirus, in ponds. We found that ddPCR had higher detection rates of bluegill eDNA in pond water than real-time PCR with either of the PCR reagents, especially at low DNA concentrations. Limits of DNA detection, which were tested by spiking the bluegill DNA to DNA extracts from the ponds containing natural inhibitors, found that ddPCR had higher detection rate than real-time PCR. Our results suggest that ddPCR is more resistant to the presence of PCR inhibitors in field samples than real-time PCR. Thus, ddPCR outperforms real-time PCR methods for detecting eDNA to document species distributions in natural habitats, especially in habitats with high concentrations of PCR inhibitors.AMER CHEMICAL SOC, May 2015, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 49(9) (9), 5601 - 5608, English[Refereed]Scientific journal
- 1. To prevent the invasion of exotic species causing a decline in an endangered endemic species, it is important to determine the distribution of both species at an early stage, when the density of the exotic species is still low, and to manage the invasion immediately. However, distinguishing between closely related species is difficult because they share similar characteristics. 2. The identification of DNA fragments sampled from a body of water (environmental DNA) has become a popular technique for rapidly determining the distribution of a target species. In this study, we analysed environmental DNA in water samples from 37 sites across the Katsura River basin in Japan. We used TaqMan real-time PCR to distinguish the Japanese giant salamander Andrias japonicus from the closely related Chinese giant salamander Andrias davidianus, which is known to invade Japanese rivers and hybridize with the Japanese species. 3. In environmental samples, we detected mtDNA of the endemic species at 25 sites and mtDNA of the exotic species at nine sites. The DNA detection sites were concentrated in the upstream region. The exotic species DNA was found beyond the limits of an earlier capturing survey. 4. Synthesis and applications. Using environmental DNA to monitor the two salamander species requires less time and effort than traditional surveys, so a wide-ranging survey can be conducted rapidly. Our results showed that performing three environmental DNA surveys for each site between autumn and winter is desirable for giant salamanders. Further collection of environmental DNA, in combination with conventional population surveys, will provide valuable information that can help protect rare endemic species in a variety of aquatic ecosystems and can help monitor the invasion of exotic species.WILEY-BLACKWELL, Apr. 2015, JOURNAL OF APPLIED ECOLOGY, 52(2) (2), 358 - 365, English[Refereed]Scientific journal
- An environmental DNA (eDNA) analysis method has been recently developed to estimate the distribution of aquatic animals by quantifying the number of target DNA copies with quantitative real-time PCR (qPCR). A new quantitative PCR technology, droplet digital PCR (ddPCR), partitions PCR reactions into thousands of droplets and detects the amplification in each droplet, thereby allowing direct quantification of target DNA. We evaluated the quantification accuracy of qPCR and ddPCR to estimate species abundance and biomass by using eDNA in mesocosm experiments involving different numbers of common carp. We found that ddPCR quantified the concentration of carp eDNA along with carp abundance and biomass more accurately than qPCR, especially at low eDNA concentrations. In addition, errors in the analysis were smaller in ddPCR than in qPCR. Thus, ddPCR is better suited to measure eDNA concentration in water, and it provides more accurate results for the abundance and biomass of the target species than qPCR. We also found that the relationship between carp abundance and eDNA concentration was stronger than that between biomass and eDNA by using both ddPCR and qPCR; this suggests that abundance can be better estimated by the analysis of eDNA for species with fewer variations in body mass.PUBLIC LIBRARY SCIENCE, Mar. 2015, PLOS ONE, 10(3) (3), e0122763 - e0122763, English[Refereed]Scientific journal
- Environmental DNA methods have been used to monitor the presence of aquatic vertebrates in natural systems, although detection of DNA in the environment is sometimes a challenge. In this study, we evaluated the effect of sample processing on the detection of a species' environmental DNA in the water. Specifically, we examined whether freezing and then thawing water samples prior to analysis was an effective method of preserving them. The detection of Common Carp DNA was lower in samples that were frozen and thawed than in samples that were not, even though there was no difference in the DNA concentration, which was included with the DNA undetectable samples. In both types of samples, the DNA detection rate tended to be higher in a 2-mu L volume of template DNA solution than in a 5-mu L volume. DNA was detected in all non-frozen samples that were analyzed using a 2-mu L template, both in three wells (three PCR replicate reactions per sample) with 40 PCR cycles and in eight wells with 55 cycles. The detection of Common Carp DNA in samples that were frozen and thawed was likely to increase through the use of the TaqMan Environmental Master Mix, which is used recently to efficiently release PCR inhibition. Our results suggest that environmental DNA detection is influenced by the processing of water samples after collection and by PCR reaction conditions. Use of non-frozen samples and a smaller DNA solution are recommended for detection of environmental DNA with quantitative PCR assays. (C) 2014 Elsevier Ltd. All rights reserved.ELSEVIER SCI LTD, Mar. 2015, BIOLOGICAL CONSERVATION, 183, 64 - 69, English[Refereed]Scientific journal
- Outbreaks of infectious diseases among freshwater fish damage the aquaculture industry. A technique for detecting pathogens in environmental water samples has recently been developed. In the present work, the monitoring of pathogenic viruses in Lake Erhai and Lake Dianchi (Yunnan, China) was performed. Yunnan possesses a rich diversity of cyprinid fish, and the aquaculture of freshwater fish is a highly prosperous industry in this region. Thus, Cyprinid herpesvirus 1, 2, and 3 as well as three rhabdoviruses, Infectious hematopoietic necrosis virus, Viral hemorrhagic septicemia virus, and the Spring viremia of carp virus, all of which have severe impacts on aquaculture, were targeted. Viruses in lake surface water were concentrated and then quantified/detected by real-time PCR/real-time reverse transcription PCR. Five of the six tested viruses were detected in lake water. Although there has been no reported viral outbreak in the survey lakes, our results indicate a potential risk for these viral diseases.SPRINGER JAPAN KK, Jan. 2015, LIMNOLOGY, 16(1) (1), 69 - 77, English[Refereed]Scientific journal
- The environmental DNA (eDNA) technique is expected to become a powerful, non-invasive tool for estimating the distribution and biomass of organisms. This technique was recently shown to be applicable to aquatic vertebrates by collecting extraorganismal DNA floating in the water or absorbed onto suspended particles. However, basic information on eDNA release rate is lacking, despite it being essential for practical applications. In this series of experiments with bluegill sunfish (Lepomis macrochirus), we examined the effect of fish developmental stage on eDNA release rate. eDNA concentration reached equilibrium 3 days after the individual fish were introduced into the separate containers, enabling calculation of the eDNA release rate (copies h(-1)) from individual fish on the assumption that the number of eDNA released from the fish per unit time equals total degradation in the container (copies h(-1)). The eDNA release rate was 3-4 times higher in the adult (body weight: 30-75 g) than in the juvenile group (0.5-2.0 g). Such positive relationship between fish size and eDNA release rate support the possibility of biomass rather than density estimation using eDNA techniques. However, the eDNA release rate per fish body weight (copies h(-1) g(-1)) was slightly higher in the juvenile than the adult group, which is likely because of the ontogenetic reduction in metabolic activity. Therefore, quantitative eDNA data should be carefully interpreted to avoid overestimating biomass when the population is dominated by juveniles, because the age structure of the focal population is often variable and unseen in the field. eDNA degradation rates (copies l(-1) h(-1)), calculated by curve fitting of time-dependent changes in eDNA concentrations after fish removal, were 5.1-15.9% per hour (half-life: 6.3 h). This suggests that quantitative eDNA data should be corrected using a degradation curve attained in the target field.PUBLIC LIBRARY SCIENCE, Dec. 2014, PLOS ONE, 9(12) (12), e114639, English[Refereed]Scientific journal
- Fluctuating water temperatures can affect fitness in fish when the opportunity to select habitats with appropriate temperature is limited. Despite the importance of the relationships between water temperature and host-pathogen interactions, reports on the susceptibility of fish to infectious viruses under conditions of changing water temperature are limited. Here, we compared the survival rates of common carp (Cyprinus carpio) infected with Cyprinid hopesvirus 3 (CyHV-3) in water in which the temperature varied from 22 degrees C +/- 3 degrees C and in water with a constant temperature of 22 degrees C or 25 degrees C. We also examined changes in concentrations of CyHV-3 DNA and cortisol released from infected fish into ambient water as indicators of CyHV-3 transmission and stress response, respectively. The survival rates of fish infected with CyHV-3 were lower, and concentrations of CyHV-3 DNA and cortisol were higher, in the fluctuating-temperature treatments than in the constant-temperature treatments. Our findings provide direct evidence that carp are highly susceptible to CyHV-3 infection when water temperatures change diurnally. Moreover, such temperature fluctuations can promote transmission of CyHV-3 in the wild. Preserving a variety of aquatic environments including water temperature may help to prevent disease outbreaks and to conserve fish populations. (C) 2014 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, Sep. 2014, AQUACULTURE, 433, 208 - 213, English[Refereed]Scientific journal
- 北海道大学高等教育推進機構 高等教育研究部 科学技術コミュニケーション教育研究部門（CoSTEP）, Jun. 2014, Japanese Journal of Science Communication, 15(15) (15), 123 - 136, Japaneseパブリックコメント・ワークショップの試行 〜「宇宙基本計画（案）」をテーマとしたワークショップの事例報告〜[Refereed]Scientific journal
- To understand the differences in the stress sensitivities between domesticated Eurasian and Japanese indigenous strains of common carp (Cyprinus carpio Linnaeus 1758), we compared concentrations of cortisol released into the water in response to handling of the two types of strains. At 0.5 and 2 h after the handling treatment, the cortisol emission was greater from the Eurasian strain than from the Japanese strain. There were no differences between the strains in the cortisol levels after 4 to 24 h. We found that Eurasian strains exposed to the unnatural stressor (i.e., handling) exhibited a higher cortisol response than the Japanese strain.SPRINGER JAPAN KK, Apr. 2014, ICHTHYOLOGICAL RESEARCH, 61(2) (2), 165 - 168, English[Refereed]Scientific journal
- Emerging infectious diseases are of growing concern in wildlife conservation and animal health. To better understand the consequences of these diseases, a key question lies in how they persist in host populations after they emerge. Using a gene expression approach, we investigated the mechanisms underlying the persistence of an emerging virus, Cyprinid herpesvirus 3 (CyHV-3), which has been spreading to wild populations of common carp (Cyprinus carpio) in Japan since 2003. Seasonal expression patterns of CyHV-3 genes in wild seropositive carp indicated that replication-related genes were transcribed only during the spring when water temperatures were permissive to CyHV-3 replication. In contrast, possible latency-related genes, which are expressed when CyHV-3 do not multiply, were also transcribed under nonpermissive conditions. These observations suggest that CyHV-3 may persist in carriers by establishing latent infection and then reactivating periodically coincident with the spring temperature increase when carp aggregate for mating, allowing successive virus transmissions between hosts during mating every year. Our results revealed that the life cycle of CyHV-3 may fit perfectly into the ecology of its host, resulting in the long-term persistence of this emerging virus in wild common carp populations.WILEY-BLACKWELL, Feb. 2014, FEMS MICROBIOLOGY ECOLOGY, 87(2) (2), 536 - 542, English[Refereed]Scientific journal
- Wiley, Jun. 2013, Animal Conservation, 16(3) (3), 324 - 330, English[Refereed]Scientific journal
- Apr. 2013, Environmental Science & Technology (Wuhan), 36(2) (2), 61 - 65, Chinese[Refereed]Scientific journal
- Knowledge of the presence of an invasive species is critical to monitoring the sustainability of communities and ecosystems. Environmental DNA (eDNA), DNA fragments that are likely to be bound to organic matters in the water or in shed cells, has been used to monitor the presence of aquatic animals. Using an eDNA-based method, we estimated the presence of the invasive bluegill sunfish, Lepomis macrochirus, in 70 ponds located in seven locales on the Japanese mainland and on surrounding islands. We quantified the concentration of DNA copies in a 1 L water sample using quantitative real-time polymerase chain reaction (qPCR) with a primer/probe set. In addition, we visually observed the bluegill presence in the ponds from the shoreline. We detected bluegill eDNA in all the ponds where bluegills were observed visually and some where bluegills were not observed. Bluegills were also less prevalent on the islands than the mainland, likely owing to limited dispersal and introduction by humans. Our eDNA method simply and rapidly detects the presence of this invasive fish species with less disturbance to the environment during field surveys than traditional methods.PUBLIC LIBRARY SCIENCE, Feb. 2013, PLOS ONE, 8(2) (2), e56584, English[Refereed]Scientific journal
- Prompt and accurate methods for assessing the species composition of given areas are indispensable in addressing the rapid loss of biodiversity. Here, we propose a method for the surveillance of fish species composition in freshwater using environmental DNA as species markers. First, the applicability of the method was demonstrated through aquarium experiments. DNA was extracted from 120 ml aquarium water, and the degenerated primers targeting the fish mitochondrial cytochrome b gene were used for amplification. PCR-amplified fragments were analysed by random cloning, and all species reared in the aquarium were detected. Next, this method was applied to natural freshwater environments. Water samples were collected from three sites in the Yura River, Japan; DNA was concentrated from 2 l of environmental water, and then amplified and cloned. Up to four species of fish were detected by sequencing 47 randomly selected clones from a single water sample. Overall, the results were consistent with previous knowledge of fish habitat utilisation. Using this method, the surveillance of fish species composition can be conducted less laboriously than with traditional methods.SPRINGER TOKYO, Aug. 2012, LIMNOLOGY, 13(2) (2), 193 - 197, English[Refereed]Scientific journal
- Cyprinid herpesvirus 3 (CyHV-3) disease is a significant threat for common and koi carp cultivators and for freshwater ecosystems. To determine the prevalence of CyHV-3 in Japanese rivers, a nationwide survey of all national class-A rivers was undertaken in the Summer of 2008. The virus was concentrated from river water samples using the cation-coated filter method. CyHV-3 DNA was detected in 90 rivers, representing 90% of 103 successfully analysed rivers. More than 100,000 copies of CyHV-3 DNA per litre of sample were detected in four rivers, higher than that reported during the Yura River outbreak in 2007. For CyHV-3-positive rivers, the log CyHV-3 density was negatively correlated with the water temperature on the sampling date and positively correlated with the suspended solids and dissolved oxygen, which are annually averaged for each river. Our results demonstrate that virus detection using molecular biology techniques is a powerful tool for monitoring the presence of CyHV-3 in natural environments. (C) 2011 Elsevier Ltd. All rights reserved.ELSEVIER SCI LTD, Aug. 2012, RESEARCH IN VETERINARY SCIENCE, 93(1) (1), 508 - 514, English[Refereed]Scientific journal
- Environmental DNA (eDNA) from aquatic vertebrates has recently been used to estimate the presence of a species. We hypothesized that fish release DNA into the water at a rate commensurate with their biomass. Thus, the concentration of eDNA of a target species may be used to estimate the species biomass. We developed an eDNA method to estimate the biomass of common carp (Cyprinus carpio L.) using laboratory and field experiments. In the aquarium, the concentration of eDNA changed initially, but reached an equilibrium after 6 days. Temperature had no effect on eDNA concentrations in aquaria. The concentration of eDNA was positively correlated with carp biomass in both aquaria and experimental ponds. We used this method to estimate the biomass and distribution of carp in a natural freshwater lagoon. We demonstrated that the distribution of carp eDNA concentration was explained by water temperature. Our results suggest that biomass data estimated from eDNA concentration reflects the potential distribution of common carp in the natural environment. Measuring eDNA concentration offers a non-invasive, simple, and rapid method for estimating biomass. This method could inform management plans for the conservation of ecosystems.PUBLIC LIBRARY SCIENCE, Apr. 2012, PLOS ONE, 7(4) (4), e35868, English[Refereed]Scientific journal
- Cyprinid herpesvirus 3 (CyHV-3) is a lethal DNA virus that infects common carp and koi. It has caused outbreak of the disease within both aquaculture and natural environmental ecosystems. However, there is not enough understanding of the distribution of CyHV-3 in the natural environments, partly because there is no suitable quantification method. In this study, we tested CyHV-3 extraction methods from sediment and then compared its abundance between sediment and water using real-time PCR. Sediment samples were taken from lake and pond, and total viral DNA was extracted using the viral elution method recommended by the US Environmental Protection Agency (manual method), as well as a commercial DNA extraction kit for soil (commercial kit method) before PCR detection. 7 of 12 (58%) and 5 of 10 (50%) sediment samples showed PCR positive signal for CyHV-3 DNA using the manual method and the commercial kit, respectively, and consistent results were obtained from the samples using the manual method between two independent primer sets. The quantification of CyHV-3 DNA in natural sediment using the manual method and external standard virus revealed that its concentration was 1.2 x 10(4) to 3.3 x 10(5) copies DNA/kg. The concentration in sediments was 46-1238 times higher than that in water from the same location, suggesting that sediment could act as a reservoir for CyHV-3 in natural freshwater environments. This is the first report of the existence of CyHV-3 in the sediment of a natural lake or pond. (C) 2011 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, Mar. 2012, VETERINARY MICROBIOLOGY, 155(2-4) (2-4), 183 - 190, English[Refereed]Scientific journal
- Error-prone polymerase chain reactions (epPCRs) are often used to introduce mutations in random mutagenesis, which has been used as a tool in protein engineering. Here, we developed a new method of epPCR using heavy water as a solvent instead of normal water (H(2)O). Rhodopsin cDNA of the Ayu fish (Plecoglossus altivelis) was used as a template and was amplified using five different conditions: (A) 100% H(2)O with no Mn(2+), (B) 100% H(2)O/0.6 mM Mn(2+), (C) 99% D(2)O with no Mn(2+), (D) 99% D(2)O/0.6 mM Mn(2+) and (E) 99% H(2) (18)O with no Mn(2+). The 13,960 (for each of the conditions A to D) and 33,504 (for condition E) base pairs were sequenced. A maximum error rate of 1.8 x 10(-3) errors/bp was detected in condition D, without any particular hot-spot mutations. A high preference for AT -> GC transitions was observed in condition D, whereas a high preference for transitions over transversions was observed in condition C. All of the mutations observed in condition E were transversions. When conditions A and C were applied to another template, the honeybee actin gene, the results were comparable to those for Ayu rhodopsin. Based on these results, the use of heavy water, instead of H(2)O, as a solvent for epPCR can introduce random mutations without positional bias, template dependency or decreased yield. Our new epPCR method, and possibly combining the use of D(2)O and H(2) (18)O, may be a powerful random mutagenesis technique. (C) 2011 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, Jan. 2012, JOURNAL OF BIOTECHNOLOGY, 157(1) (1), 71 - 74, English[Refereed]Scientific journal
- We report the expression of two mRNA variants, alpha and beta, of the period (per) gene in honeybees. These have not been found in other insects. The genomic DNA sequence of the per gene in the Japanese honeybee (Apis cerana japonica) showed that the variants are produced by alternative splicing of an intron. Total per mRNA in the brain increased in the dark period and decreased in the light, whereas mRNA of the muscle showed an opposite phase oscillation. No significant rhythmic expression of per mRNA was observed in the compound eye and midgut. The expression ratio of the alpha and beta variants of per mRNA was investigated at zeitgeber times (ZTs) 6 and 18 (the middle of the day and night, respectively) under 12: 12 h light-dark (LD) conditions. The per alpha variant increased in the dark in both organs, whereas expression of the beta variant showed an opposite pattern between the organs. The higher total per mRNA expression in muscle in the light period was due to an increase in the beta variant. Such expression patterns suggest that there is a functional differentiation between the alpha and beta variants. Furthermore, we found that other species of Hymenoptera possess homologs of the per alpha and beta variants, suggesting that they have a specific function in this order of insects.TAYLOR & FRANCIS LTD, 2012, BIOLOGICAL RHYTHM RESEARCH, 43(2) (2), 125 - 135, English[Refereed]Scientific journal
- Lake Erhai is located in a low latitude, high altitude region in Yunnan province, China, and contains diverse endemic aquatic organisms. This study provides baseline information on the spatiotemporal water temperature distribution for examining the thermal environment for aquatic organisms in Lake Erhai. Temperature observation using temperature loggers was conducted from March to June 2009, covering the spawning period of most cyprinid fishes, the dominant taxonomic group of fish in the lake. During the daytime, the hourly average temperature was different between the surface and the bottom layer both in the littoral and pelagic zones; those differences disappeared during the nighttime, indicating nocturnal vertical water mixing. Daily average temperature, as well as intraday temperature variation, was negatively correlated with the site depth both in the surface and the bottom layers, suggesting that the littoral zone is warmer than the pelagic zone but less stable as a thermal habitat for aquatic species. Based on its geological location and depth, Lake Erhai could be classified as a polymictic lake, which tends to be well mixed; however, our study results indicate a high heterogeneity in water temperature. This specific thermal environment supports local fauna and flora and thus must be conserved to ensure their survival.FRESHWATER BIOLOGICAL ASSOC, 2012, INLAND WATERS, 2(3) (3), 129 - 136, English[Refereed]Scientific journal
- To predict outbreaks of infectious disease and to prevent epidemics, it is essential not only to conduct pathological studies but also to understand the interactions between the environment, pathogen, host and humans that cause and spread infectious diseases. Outbreaks of mass mortality in carp caused by Cyprinid herpesvirus 3 (CyHV-3), formerly known as koi herpesvirus (KHV), disease have occurred worldwide since the late 1990s. We proposed an environment-KHV-carp-human linkage as a conceptual model for "environmental diseases" and specify research subjects that might be necessary to construct and shape this linkage.SPRINGER TOKYO, Nov. 2011, ECOLOGICAL RESEARCH, 26(6) (6), 1011 - 1016, English[Refereed]Scientific journal
- The littoral zone of lakes and lagoons is often used by fish for feeding or reproduction. However, the large changes in temperature that are typical of natural environments, including the littoral zone, represent a potential stressor for fish. Despite the importance of this habitat, little is known about the effect of daily temperature fluctuations on the stress responses of fish. We monitored daily temperature changes in the near-shore and offshore regions of a natural lagoon between May and July 2008-2010. We observed large temperature fluctuations more frequently in the near-shore zone than the offshore zone. We then exposed common carp (Cyprinus carpio) to a temperature regime similar to that observed in the near-shore zone and measured the levels of cortisol released into the water. The rate of cortisol release increased when carp were exposed to an increase in temperature of similar to 0.6A degrees C/h over a 5-h period. Conversely, there was no change in the rate of release when temperatures decreased. Our results highlight the importance of maintaining high temporal resolution when evaluating the stress response to daily fluctuations temperature.SPRINGER, Oct. 2011, HYDROBIOLOGIA, 675(1) (1), 65 - 73, English[Refereed]Scientific journal
- The disease caused by cyprinid herpesvirus-3 (CyHV-3) severely impacts the natural freshwater ecosystem and damages carp and koi farming, however, the pathway of CyHV-3 transmission remains unclear. It is possible that the virus adheres to plankton, which then facilitate viral movement and transmission, and therefore, it is hypothesised that plankton are involved in the disease dynamics. In this study, plankton were collected at eight sites in the Iba-naiko lagoon; we detected and quantified CyHV-3 DNA from plankton samples. The results of the correlation analysis showed a significant positive correlation between CyHV-3 copies and the number of Rotifera, suggesting that CyHV-3 binds to and/or is concentrated by Rotifera. Our results suggest that plankton affect viral ecology in the natural environment. (C) 2010 Elsevier Ltd. All rights reserved.Lead, ELSEVIER SCI LTD, Jun. 2011, RESEARCH IN VETERINARY SCIENCE, 90(3) (3), 530 - 532, English[Refereed]Scientific journal
- Emerging infectious diseases are major threats to wildlife populations. To enhance our understanding of the dynamics of these diseases, we investigated how host reproductive behavior and seasonal temperature variation drive transmission of infections among wild hosts, using the model system of cyprinid herpesvirus 3 (CyHV-3) disease in common carp. Our main findings were as follows: (1) a seroprevalence survey showed that CyHV-3 infection occurred mostly in adult hosts, (2) a quantitative assay for CyHV-3 in a host population demonstrated that CyHV-3 was most abundant in the spring when host reproduction occurred and water temperature increased simultaneously and (3) an analysis of the dynamics of CyHV-3 in water revealed that CyHV-3 concentration increased markedly in breeding habitats during host group mating. These results indicate that breeding habitats can become hot spots for transmission of infectious diseases if hosts aggregate for mating and the activation of pathogens occurs during the host breeding season. The ISME Journal (2011) 5, 244-251; doi:10.1038/ismej.2010.123; published online 26 August 2010NATURE PUBLISHING GROUP, Feb. 2011, ISME JOURNAL, 5(2) (2), 244 - 251, English[Refereed]Scientific journal
- Foragers of the Japanese honeybee (Apis cerana japonica) were attracted by flowers of an oriental orchid (Cymbidium floribundum) and were observed to carry the pollinia on their scutella. After the removal of pollinia from the flowers, their labial color changed from white to reddish brown. Both artificial removal of pollinia and ethrel treatment of the flowers also induced this labial color change. Labia in color-changed flowers showed a decreased reflectance of wavelengths less than 670 nm compared to control intact flower. Both reflectance irradiance spectra and ultraviolet photographs showed that only the nectar guide in white (unchanged) flowers reflected ultraviolet light, and that this reflectance decreased with labial color change. Dual choice experiments showed that the honeybee foragers preferentially visited flowers having white labia rather than reddish brown. We suggest that Japanese honeybees discriminate between the floral phases of C. floribundum using color vision.ZOOLOGICAL SOC JAPAN, Dec. 2010, ZOOLOGICAL SCIENCE, 27(12) (12), 901 - 906, English[Refereed]Scientific journal
- Lakeshore developments change the physicochemical properties of the underwater environment by altering shore morphometry, which may have significant effects on spatial variation and temporal stability in water temperature. Spatiotemporal temperature changes are costly to fish in terms of subsequent thermoregulatory behavior and acclimation; therefore, thermal conditions have a heavy impact on the biological function of fishes. Spatiotemporal variation and stability of water temperatures along cross-shore transects in the littoral zone (within 100 m from shore) were monitored and compared on two lakeshores with different cross-shore depth profiles. One shore was associated with a retaining wall and a relatively deep, flat bottom (steep shore), whereas the other extended offshore at a gentle gradient (gentle shore). Water temperature was more spatially variable on the gentle shore than the steep shore [1.44 +/- A 0.47 and 0.20 +/- A 0.14A degrees C (mean +/- A SD), respectively], but a stable temperature range (i.e., the range of temperatures continuously observed on each shore for 48 h) was maintained only on the gentle shore during seasonal temperature decline. These results suggest that gentle shores have higher potential to provide a wider range of thermal options, allowing fish to fine-tune thermoregulatory behavior and acclimate more efficiently to temperature changes.SPRINGER TOKYO, Apr. 2010, LIMNOLOGY, 11(1) (1), 71 - 76, English[Refereed]Scientific journal
- The molecular mechanisms of the endogenous circadian clocks that allow most animals to adapt to environmental cycles have recently been uncovered. The draft genome of the ascidian, Ciona intestinalis, a model animal that is close to vertebrates, has been described. However, the C. intestinalis genome lacks the canonical clock genes such as Per, Bmal and Clock that are shared by vertebrates and insects. Here, we found the circadian rhythms at the physiological and molecular levels. The oxygen consumption rate was lower during the light phase and higher during the dark phase during a day, and the rhythm highly damped and continued under constant darkness. From the microarray analysis, the 396 spots (1.8% of the total; corresponding to 388 clones) were extracted as candidates for circadian expression. We confirmed the circadian expression of several candidate genes by northern blotting. Furthermore, three of four rhythmic expressed genes showed phase-shifts to prolonged light period. However, most of known clock genes did not oscillate. These data suggest that C. intestinalis have a unique molecular circadian clock and the daily environmental change is not such a strong effect for sea squirt in its evolution when compared to vertebrates and insects.OXFORD UNIV PRESS, Feb. 2010, JOURNAL OF BIOCHEMISTRY, 147(2) (2), 175 - 184, English[Refereed]Scientific journal
- Cyprinid herpesvirus 3 (CyHV-3), a lethal DNA virus that spreads in natural lakes and rivers, infects common carp and koi. We established a quantification method for CyHV-3 that includes a viral concentration method and quantitative PCR combined with an external standard virus. Viral concentration methods were compared using the cation-coated filter and ultrafiltration methods. The recovery of virus-like particles was similar for the two methods (cation-coated filter method, 44% +/- 19%, n = 3; ultrafiltration method, 50% +/- 3%, n = 3); however, the former method was faster and more suitable for routine determinations. The recovery of seeded CyHV-3 based on the cation-coated filter method varied by more than 3 orders of magnitude among the water samples. The recovery yield of CyHV-3 was significantly correlated with that of the seeded lambda phage, and the average ratio of lambda to the CyHV-3 recovery yield was 1.4, indicating that lambda is useful as an external standard virus for determining the recovery yield of CyHV-3. Therefore, to quantify CyHV-3 in environmental water, a known amount of lambda was added as an external standard virus to each water sample. Using this method, CyHV-3 DNA was detected in 6 of the 10 (60%) types of environmental water tested; the highest concentration of CyHV-3 DNA was 2 x 10(5) copies liter(-1). The lowest recovery limit of CyHV-3 DNA was 60 copies liter(-1). This method is practical for monitoring CyHV-3 abundance in environmental water.AMER SOC MICROBIOLOGY, Jan. 2010, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(1) (1), 161 - 168, English[Refereed]Scientific journal
- The seasonal distribution of the cyprinid herpesvirus 3 (CyHV-3) in Lake Biwa, Japan, was investigated. CyHV-3 was distributed all over the lake 5 years after the first outbreak. The mean concentration of CyHV-3 in water showed annual oscillation, with a peak in the summer and a trough in winter. Our results suggested that CyHV-3 is present at high density in reductive environments, such as reed zones and turbid or eutrophic water.AMER SOC MICROBIOLOGY, Nov. 2009, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(21) (21), 6900 - 6904, English[Refereed]Scientific journal
- The disease caused by cyprinid herpesvirus 3 (CyHV-3) brings catastrophic damages to cultivated carp and koi and to natural carp populations; however, the dynamics of the virus in environmental waters are unclear. In July 2007, CyHV-3 DNA was detected in a dead common carp collected from the Yura River in Kyoto Prefecture, Japan, and this was followed by mass mortality. We collected water samples at eight sites along the Yura River for 3 months immediately after confirmation of the disease outbreak and attempted to detect and quantify CyHV-3 DNA in the water samples using molecular biological methods. The virus concentration was carried out by the cation-coated filter method, while the purification of DNA from the samples was achieved using phenol-chloroform extraction and a commercial DNA extraction kit. CyHV-3 was detected by PCR using six sets of conditions, three sets of primers (SphI-5, AP, and B22Rh exon 1), and two volumes of template DNA, and was quantified using real-time PCR. Our results indicate broader distribution of CyHV-3, even though dead fish were found only in a limited area; moreover, the virus was present at high levels in the river not only during the mass mortality caused by the disease but also for at least 3 months after the end of mass mortality. Our results suggest the possibility of infection by CyHV-3 via environmental water. The sequences of CyHV-3 collected from the Yura River matched perfectly with that of the CyHV-3 Japanese strain, suggesting that they share the same origin. (C) 2008 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, Mar. 2009, VETERINARY MICROBIOLOGY, 135(3-4) (3-4), 261 - 266, English[Refereed]Scientific journal
- 玉川大学ミツバチ科学研究所, Dec. 2006, ミツバチ科学, 27(1) (1), 19 - 22, Japanese大阪府下のミツバチ生息状態Research institution
- 日本魚類学会, Nov. 2005, Japan. J. Ichthyol., 52(2) (2), 91 - 106, Japanese[Refereed]Scientific journal
- Five cone opsin genes of landlocked ayu fish (Plecoglossus altivelis) were cloned, and the expression patterns of these genes were investigated. AYU-LWS, -RH2-1, -RH2-2, -SWS1-1, and -SWS1-2 were isolated and had high (more than 75%) identity with red, green, green, UV, and UV-sensitive opsin, respectively, genes of other fish reported previously. The results of Southern blotting experiments showed that each gene is present as a single copy. Gene expression was measured by RT-PCR using four populations collected from rivers and a lake in spring and summer. The results of the RT-PCR experiment showed that AYU-SWS1-2 was highly expressed, whereas AYU-SWS1-1 was scarce. Two RH2 opsins were expressed simultaneously in the same individual, and the expression ratio between these opsins changed among populations. In situ hybridization revealed that AYU-LWS and -RH2-1 were expressed in the double cones and that AYU-RH2-2 and -SWS1-2 were expressed in the long and short single cones (LSC and SSC), respectively. It was shown that an individual ayu expresses two RH2 opsins simultaneously in different types of cone cells. © 2004 Elsevier Inc. All rights reserved.Lead, 2, Feb. 2005, Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 140(2) (2), 197 - 205, English[Refereed]Scientific journal
- 玉川大学ミツバチ科学研究所, Nov. 2003, ミツバチ科学, 24(3) (3), 115 - 118, JapaneseキンリョウヘンCymbidium floribundum唇弁の着色がニホンミツバチApis cerana japonicaの訪花行動にあたえる影響Research institution
- Amplified fragments encoding exon-4 of opsin cDNAs were cloned from the retina of landlocked ayu (Plecoglossus altivelis), and sequenced. On the basis of the sequence homology to previously characterized fish visual pigments, one clone was identified as rod opsin (AYU-Rh), and two clones as green (AYU-G1, -G2), one as red (AYU-R) and two as ultraviolet (AYU-UV1, -UV2) cone opsins. The 335-amino acid sequence deduced from the full-length cDNA of AYU-Rh included residues highly conserved in vertebrate rhodopsins and showed the greatest degree (88%) of similarity with salmon rhodopsin. Southern blotting analysis indicated that ayu possess two rhodopsin genes, one encoding visual rhodopsin (AYU-Rh) and the other non-visual extra-ocular rhodopsin (AYU-ExoRh). RT-PCR experiments revealed that AYU-Rh was expressed in the retina and AYU-ExoRh in the pineal gland. In situ hybridization experiments showed that the mRNA of AYU-Rh was localized only in rod cells not in cone cells. Lake and river type landlocked ayu having different amounts of retinal and 3-hydroxyretinal in their retinas expressed a rhodopsin (AYU-Rh) of identical amino acid sequence. (C) 2003 Elsevier Science Inc. All rights reserved.Lead, PERGAMON-ELSEVIER SCIENCE LTD, Apr. 2003, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 134(4) (4), 559 - 570, English[Refereed]Scientific journal
- Two kinds of PCR-product cDNAs that encode premature lysozyme peptides (Rs-Lys1 and Rs-Lys2) were cloned from workers of a Japanese damp-wood termite, Reticulitermes speratus. The Rs-Lys1 and Rs-Lys2 cDNAs encoded deduced sequences of 170 and 164 amino acids, respectively. Alignment of these sequences with those of other insect lysozymes showed that the cDNAs encode lysozyme homologues with putative signal peptides, insertions eight amino acids long, and a relatively long C-terminus (13-17 amino acids). A maximum likelihood tree, constructed using the cDNA sequences, indicated that the termite lysozymes are related to those of mosquitoes and lepidopterans. Southern-blotting analysis identified single copies of these lysozyme genes in the termite. Reverse transcript (RT)-PCR and in situ hybridization experiments showed that Rs-Lys1 and Rs-Lys2 are expressed in the salivary glands of worker termites. Here, we discuss the possible digestive function of these lysozymes. (C) 2002 Elsevier Science Ltd. All rights reserved.PERGAMON-ELSEVIER SCIENCE LTD, Dec. 2002, INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 32(12) (12), 1615 - 1624, English[Refereed]Scientific journal
- Lead, Korean Society of PhotoScience, 2002, Journal of Photoscience, 9(2) (2), 269 - 271, EnglishStudies of opsin genes in a smelt fish, Ayu (Plecoglossus altivelis)International conference proceedings
- Lead, Faculty of Science, Kyoto University, 2002, Memoirs of the Faculty of Science, Kyoto University (Series of Biology), 18, 1 - 13, EnglishPhylogenetic relationship among osmerid and salangid fish inferred from mitochondrial cytochrome b gene sequencesResearch institution
- Vertebrate ancient (VA) opsin of nonvisual pigment in fishes was reported to exist in two isoforms, i.e., short and long variants with an unusual predicted amino acid sequence length compared to vertebrate visual opsins. Here we cloned an isoform (Pal-VAM) of VA opsin showing the usual opsin length in addition to the long type isoform (Pal-VAL) from a smelt fish, Plecoglossus altivelis. Pal-VAM and Pal-VAL were composed of 346 and 387 amino acids, respectively. The deduced amino acid sequences of these variants were identical to each other within the first 342 residues, but they showed divergence in the carboxyl-terminal sequence. Pal-VAL corresponded to the long isoform found in zebrafish and carp, and Pal-VAM was identified as a new type of VA opsin variant. Southern blotting experiments indicated that the VA opsin gene of the smelt is present as a single copy, and RT-PCR analysis revealed that Pal-VAM and Pal-VAL mRNA were expressed in both the eyes and brain. In situ hybridization showed that Pal-VAM and Pal-VAL mRNA are expressed in amacrine cells in the retina. Pal-VAM is a new probably functional nonvisual photoreceptive molecule in fish. (C) 2002 Elsevier Science.Lead, ACADEMIC PRESS INC ELSEVIER SCIENCE, Jan. 2002, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 290(1) (1), 280 - 286, English[Refereed]Scientific journal
- The polymerase chain reaction (PCR) was performed with cDNA from the silkworm compound eye and primers designed to amplify a region of the opsin-encoding cDNA. Two distinct fragments which encode two opsins, designated Bomopsin1 and Bomopsin2, were isolated. The fragments of Bomopsin1 and 2 consisted for 207 nucleotides (nt) encoding a sequence of 69 amino acids (aa), and 213 nt encoding 71 aa, respectively. The deduced amino acid sequences of Bomopsin1 and 2 showed 100-68% identity and 94-63% identity to the corresponding region of other insects opsin. the high identities between Bombyx and Manduca opsins suggest that Bomopsin1 is a green-sensitive pigment and Bomopsin2 is a UV-sensitive pigment in the silkworm eye.JAPAN SOC APPL ENTOMOL ZOOL, Feb. 1998, APPLIED ENTOMOLOGY AND ZOOLOGY, 33(1) (1), 199 - 204, English[Refereed]Scientific journal
- Lead, Jan. 2025, ミルシル, 18(1) (1), 15 - 17, Japanese環境DNA調査の広がりと今後の期待[Invited]Introduction other
- Lead, Dec. 2024, 中等教育資料, (1063) (1063), 2 - 3, Japanese教科書的には正しくないことがおもしろい[Invited]Introduction other
- Abstract With the aim to gather and synthesize knowledge regarding environmental DNA analysis and its applications, The eDNA Society International Meeting 2023 was held in Otsu, Shiga, Japan, from May 17th to 19th with more than 252 participants from 19 countries. During the three core days, there were three plenary sessions with nine leading researchers in the field as invited speakers, as well as general oral sessions, a poster session, workshops presented by the society's corporate companies, and opening and closing ceremonies. The meeting had been postponed multiple times due to the COVID‐19 pandemic but was finally held in‐person, with options for online attendance, in front of Lake Biwa. This is a memorial site where the first field test for eDNA analysis in Japan was conducted. Here, we report the record of the meeting by sharing the highlights of the three core days. The proposed meeting theme, “Moving from Knowledge into Practice,” was well achieved during the meeting with energetic participants and enthusiastic discussion, which was stimulated by interesting presentations and lectures spanning from basic technical developments to practical applications.Wiley, Nov. 2023, Environmental DNA, 5, 1191 - 1195, EnglishMeeting report
- Lead, Apr. 2022, Pest Control, 198, 20 - 25, JapaneseAssessing the distribution of invasive alien ants by environmental DNA analysis of soils (in Japanese)[Invited]Introduction other
- Lead, Feb. 2020, JMOA Report, (19) (19), 1 - 10, JapaneseAttempt to obtain information on aquatic organisms using environmental nucleic acids[Invited]Introduction other
- Nov. 2019, KAGAKU, 89(11) (11), 1029 - 1035, JapaneseDevelopment and application of environmental DNA analysis for macro-organisms[Invited]Introduction commerce magazine
- Mar. 2019, Seiryu-Seiko, 146, 10 - 11, JapaneseEnvironmental DNA: A novel monitoring tool for aquatic organisms[Invited]Introduction other
- Mar. 2019, Kagaku to Seibutsu (Chemistry and Organisms), 57(3) (3), 181 - 186, JapaneseOutline of environmental DNA analysis and detection of rare species: distribution of endangered species discovered by a cup of water[Invited]Introduction scientific journal
- 九州環境管理協会, 2019, 環境管理 = Environmental evaluation, (48) (48), 4 - 14, Japanese環境DNA技術の現状と今後の展望(座談会)—特集 環境DNAを考える
- Apr. 2018, Journal of Japan Society on Water Environment, 41A(4) (4), 123 - 127, JapaneseEcological surveys of macro-organisms based on species-specic detection of environmental DNA[Invited]Introduction scientific journal
- The Institution of Professional Engineers, Japan, Mar. 2018, IPEJ Journal, 30(3) (3), 20 - 21, JapaneseEnvironmental DNA: A new tool for biological monitoring with a bottle of waterIntroduction scientific journal
- Feb. 2018, Aquabiology, 40(1) (1), 17 - 22, JapaneseEvaluating the efficiency of environmental DNA through tnak experiments and field surveys[Invited]Introduction commerce magazine
- Feb. 2018, Aquabiology, 40(1) (1), 47 - 53, JapaneseThe potential of environmental DNA for the estimation of population abundance and biomass[Invited]Introduction commerce magazine
- Feb. 2018, Aquabiology, 40(1) (1), 3 - 8, JapaneseWhat is environmental DNA?[Invited]Introduction commerce magazine
- Dokkyo University School of Medicine, 2018, Dokkyo Journal of Medical Sciences, 45(2) (2), 85, EnglishEnvironmental DNA and its application in the detection and control of fish and water borne zoonosis: Opisthorchiasis in LAOs and schistosomiasis in MadagascarReport scientific journal
- Dec. 2017, Environmental Conservation Engineering, 46(12) (12), 624 - 629, JapaneseCurrent developmental status of environmental DNA monitoring for aquatic environments[Invited]Introduction scientific journal
- Dec. 2017, Environmental Conservation Engineering, 46(12) (12), 648 - 652, JapaneseIssues and prospects of environmental DNA monitoring methods[Invited]Introduction scientific journal
- 10 Nov. 2017, 水産海洋研究, 81(4) (4), 305‐307, Japanese大量シーケンスと標準DNAを利用した魚類環境DNAの網羅的・定量的モニタリング:京都府舞鶴湾での解析事例
- Jun. 2017, Education Study Group for Biology of High School in Hyogo Prefecture, 41, 2 - 5, JapaneseBiological survey by high school students using environmental DNA analysis[Invited]Introduction other
- Environmental DNA analysis for micro- and macro-organisms is rapidly developing. Environmental DNA means total DNA present in environmental media such as water or soil, and includes DNA contained in the organisms themselves and extra-organism DNA of macro-organisms. Analysis of environmental DNA can be divided into two methods, species-specific detection and meta-barcoding, which can be used according to each purpose. Applicable subjects are all organisms (including viruses in this case) with DNA as genes, and application to rivers, ponds, lakes and marines has been reported. In this paper, the present situation of environmental DNA analysis of macro organisms is described, and the possibility of application to infectious disease studies and the problems to be solved are discussed.The Japanese Society for Virology, Jan. 2017, Virus, 66(2) (2), 171 - 178, Japanese[Invited]Introduction scientific journal
- CMC Publishing, Jun. 2016, BIOINDUSTRY, 33(6) (6), 60 - 65, JapaneseAquatic species census using environmental DNA[Invited]Introduction commerce magazine
- 2016, 日本生態学会大会講演要旨(Web), 63rd環境DNAメタバーコーディングを用いた淀川の魚類相モニタリング
- Jan. 2016, Jikken Igaku (Experimental Medicine), 34(1) (1), 103 - 107, JapaneseImpact of environmental DNA analysis[Invited]Introduction commerce magazine
- Tohoku University, 2016, Japanese Journal of Ecology, 66(3) (3), 581 - 582, JapaneseReport scientific journal
- Tohoku University, 2016, Japanese Journal of Ecology, 66(3) (3), 621 - 624, JapaneseReport scientific journal
- PUBLIC LIBRARY SCIENCE, Mar. 2015, PLOS ONE, 10(3) (3), EnglishOthers
- Ishiyaku Publishers, Icn., Jun. 2014, Journal of Clinical and Experimental Medicine, 249(12) (12), 1264 - 1269, Japanese[Invited]Introduction commerce magazine
- ANALYSIS OF mRNA VARIANTS OF PERIOD GENES IN APIS CERANA AND OTHER HYMENOPTERA INSECTS(Physiology&Biochemistry,Abstracts of papers presented at the 74^
Annual Meeting of the Zoological Society of Japan) :Zoological Society of Japan, 2003, Zoological science, 20(12) (12), 1593 - 1593, English
■ Books And Other Publications- Single work, 岩波書店, 2022, Japanese, ISBN: 9784000297158環境DNA入門 ただよう遺伝子は何を語るかGeneral book
- Contributor, pp. 756-765. "Environmental DNA analysis", NTS Inc., Jan. 2022, JapaneseCutting-edge Analytical Techniques, 2nd EditionDictionary or encycropedia
- Joint work, pp. 249-253. column "Standardization of Methods in Environmental DNA Analysis", Kyoritsu Syuppan Co. LTD., Mar. 2021Environmental DNA: Deciphering the True Nature of Ecosystems (Ed. by H. Doi and M. Kondoh) (in Japanese)
- Contributor, pp. 1-35. Chapter 1 "Overview of Environmental DNA Analysis", Kyoritsu Syuppan Co. LTD., Mar. 2021Environmental DNA: Deciphering the True Nature of Ecosystems (Ed. by H. Doi and M. Kondoh) (in Japanese)
- Contributor, pp. 492-493, "Environmental DNA", Maruzen Publishing, Oct. 2018, JapaneseThe Encyclopedia of Ichthyology (in Japanese)Dictionary or encycropedia
- Contributor, pp.491-495. Random Mutagenesis by Error-Prone Polymerase Chain Reaction Using a Heavy Water Solvent, Springer New York, Oct. 2016, English, ISBN: 9781493964703Scholarly book
- Contributor, pp. 39-51., Kyoritsu Syuppan, Mar. 2016, JapanesePathogenic mechanism of infectious diseases. In Ecology of Infectiuos diseases (Ecological Society of Japan ed.), Kyoritsu Syuppan (Tokyo),Scholarly book
- Contributor, pp. 159-161. コラム「水を調べるだけで生き物がわかる！環境DNAを利用した生物分布モニタリング法」, 朝倉書店, Jun. 2014, Japanese身近な水の環境科学 実習・測定編 （日本陸水学会東海支部会編）Textbook
- Contributor, pp. 75-86. "Environmental Change of the Lake and Infectious Disease", 松香堂書店, Mar. 2014, JapaneseOur Lakes: From the Present towards a Future Perspective (RIHN-China Study Series No.3: Kawabata Z., Kong H., Wu D., FUKUSHI Y., Kubota J. eds)Scholarly book
- Contributor, pp. 139-151. "An On-Site Empirical Study on the Role of Aquatic Plants in Improving Lakefromt Water Quality", 松香堂書店, Mar. 2014, ChineseOur Lakes: From the Present towards a Future Perspective (RIHN-China Study Series No.3: Kawabata Z., Kong H., Wu D., FUKUSHI Y., Kubota J. eds)Scholarly book
- Contributor, pp. 68-69. 第4章 4.4 「環境DNAを用いた水中動物相のモニタリング」, 総合地球環境学研究所, Jan. 2014, JapaneseDictionary or encycropedia
- Contributor, pp140-164 魚類の多様性とオプシン遺伝子, 京都大学学術出版会, 2007, Japanese, ISBN: 9784876988273生物の多様性ってなんだろう-生命のジグソーパズル (京都大学総合博物館・京都大学生態学研究センター編)General book
- Contributor, pp 108-117「DNAチップを用いた生物時計機能解析－ショウジョウバエの交尾行動リズムとホヤの体内時計」, シーエムシー出版, Sep. 2006, Japanese, ISBN: 9784882315902DNAチップ活用テクノロジーと応用 (久原哲編)Scholarly book
- Contributor, pp90-109 「見える世界が魚を変える－魚類の多様性と視覚適応」, 丸善, 2003, Japanese, ISBN: 9784621072189生物多様性のすすめ－生態学からのアプローチ(大串隆之編)General book
- AquaEcOmics 2025, Mar. 2025, EnglishEnhancing eDNA metabarcoding accuracy: mitigating PCR bias with droplet PCROral presentation
- AquaEcOmics 2025, Mar. 2025, EnglishQuickConc: a novel cationic-assisted eDNA capture method for enhanced biodiversity monitoringOral presentation
- The 94th Annual Meeting of Japanese Society for Parasitology, Mar. 2025, EnglishEnvironmental surveillance of Opisthorchis viverrini in endemic areas of ChampasackOral presentation
- 第72回日本生態学会大会, Mar. 2025, Japanese環境DNA分析を用いた水田におけるカエル類の個体数密度と景観要因の関係の解析Poster presentation
- 第72回日本生態学会大会, Mar. 2025, Japanese環境DNAを用いたタイ肝吸虫検出系の開発および感染症生態学への応用Poster presentation
- 第72回日本生態学会大会, Mar. 2025, Japanese温暖化は琵琶湖の魚類の分布にどのような影響を与えるのか？Poster presentation
- 第72回日本生態学会大会, Mar. 2025, Japanese環境DNAにおける精子特異的メチル化状態を利用したカワバタモロコの繁殖検出Poster presentation
- 2nd Australian and New Zealand Environmental DNA Conference, Feb. 2025, EnglishTracking reproductive activities using environmental DNA[Invited]Keynote oral presentation
- 2nd Australian and New Zealand Environmental DNA Conference, Feb. 2025, EnglishQuickConc: A rapid, efficient, and power-free eDNA concentration method for diverse aquatic environmentsOral presentation
- 2nd Australian and New Zealand Environmental DNA Conference, Feb. 2025, EnglishEnvironmental DNA can detect the hybridPoster presentation
- 2nd Australian and New Zealand Environmental DNA Conference, Feb. 2025, EnglishNegative effects of farmland abandonment on the reproduction of a small salamander (Hynobius setouchi)Poster presentation
- International Symposium on Environmental DNA for Conservation and Biomonitoring in Southeast Asia 2025 (eDNAConBio 2025), Feb. 2025, EnglishQuickConc: A rapid, efficient, and power-free eDNA concentration method for diverse aquatic environmentsOral presentation
- 日本地衣学会第23回大会, Nov. 2024, Japanese環境DNAメタバーコーディング法を活用した新たな樹上生物多様性モニタリング法の開発―樹幹流を利用して―Oral presentation
- The 7th Annual Meeeting of The eDNA Society, Dec. 2024, EnglishDevelopment of a rapid, efficient, and power-free eDNA concentration method (QuickConc)Poster presentation
- The 7th Annual Meeting of The eDNA Society, Dec. 2024, JapaneseAttempt to understand crustaceans inhabiting the bottom of a dam reservoir by eDNA analysisPoster presentation
- The 7th Annual Meeting of The eDNA Society, Dec. 2024, JapanesePotential of droplet PCR to ameliorate biased amplification in library preparation for eDNA metabarcoding[Invited]Nominated symposium
- The 7th Annual Meeting of The eDNA Society, Dec. 2024, JapaneseEnvironmental DNA can detect a hybrid[Invited]Nominated symposium
- The 7th Annual Meeting of The eDNA Society, Dec. 2024, JapaneseInfluence of homogenization methods on lichen species detection from environmental DNA metabarcodingPoster presentation
- The 7th Annual Meeting of The eDNA Society, Dec. 2024, EnglishDevelopment of a detection tool and distribution analyses for the nuisance alien diatom Cymbella janischiiPoster presentation
- The 7th Annual Meeting of The eDNA Society, Dec. 2024, JapaneseIntroducing Epigenetics into Environmental DNA Analysis: Opportunities and Challenges[Invited]Nominated symposium
- The 7th Annual Meeting of The eDNA Society, Dec. 2024, EnglishElucidating the spatiotemporal distribution dynamics of black bass species in Lake BiwaPoster presentation
- The 7th Annual Meeting of The eDNA Society, Dec. 2024, EnglishEstimation of environmental factors regulating the distribution of Setouchi salamanders in abandoned farmlandPoster presentation
- The 7th Annual Meeting of The eDNA Society, Dec. 2024, EnglishStudy on the distribution of native and non-native bitterling fish in the Okayama PlainPoster presentation
- The 7th Annual Meeting of The eDNA Society, Dec. 2024, EnglishCollection of environmental DNA from stemflow for detecting terrestrial mammalsPoster presentation
- The 7th Annual Meeting of The eDNA Society, Dec. 2024, EnglishDevelopment of detection assay for Schistosoma mekongi eDNA and its application in the fieldPoster presentation
- The 7th Annual Meeting of The eDNA Society, Dec. 2024, EnglishEstimation of distribution factors of Opisthorchis viverrini, in Savannakhet province, Lao PDRPoster presentation
- 第４７回日本分子生物学会年会, Nov. 2024, Japanese迅速、効率的なeDNA核酸濃縮手法(QuickConc)の開発Poster presentation
- The 11th International Flatfish Symposium, Nov. 2024, EnglishMonitoring the distribution of hatchery-reared spotted halibut (Verasper variegatus) juveniles after release using environmental DNAOral presentation
- Biodiversity Genomics Conference 2024, Oct. 2024, EsperantoQuickConc: Fast, efficient, power-free eDNA captureOral presentation
- The 14th Lao National Health Research Forum, Oct. 2024, EnglishSeasonality of Opisthorchis viverrini environmental DNA detection in endemic areas of Champasack, Lao-PDR
- The 14th Lao National Health Research Forum, Oct. 2024, EnglishEco Health/One Health towards eradication of opisthorchiasis using the environmental DNA detection as an evaluation tool
- The 29th Biennial Conference of Asian Association for Biology Education, Oct. 2024, EnglishInvestigating the habitat of Aphelocheirus nawai using environmental DNA analysis
- The 29th Biennial Conference of Asian Association for Biology Education, Oct. 2024, EnglishEvaluation of the effectiveness of environmental education using environmental DNA analysis: Is it effective even for people who hesitate to touch living things?Poster presentation
- 21st International Congress for Tropical Medicine and Malaria, Sep. 2024, EnglishEnvironmental DNA approach in Eco Health/One Health towards eradication of opisthorchiasis
- 応用生態工学会 第２７回さいたま大会, Sep. 2024, Japanese迅速、効率的、かつ電力不要なeDNA核酸濃縮手法(QuickConc)の開発Oral presentation
- 応用生態工学会 第２７回さいたま大会, Sep. 2024, Japanese環境DNAを用いたセトウチサンショウウオ(Hynobius setouchi)の生息に影響する環境要因の推定Poster presentation
- Salamander Meeting 2024, Jul. 2024, EnglishEstimating suitable breeding habitat for the Setouchi salamander (Hynobius setouchi) based on environmental DNA surveysPoster presentation
- 令和6年度日本水産学会春季大会, Mar. 2024, Japaneseクロダイの産卵検出における環境DNAの核/ミトコンドリア比の有効性Poster presentation
- 第71回日本生態学会大会, Mar. 2024, Japanese環境DNAのメチル化解析に基づく魚類繁殖活動の検出Poster presentation
- 第4回ウナギ学の現状, Mar. 2024, Japanese環境DNAを用いたニホンウナギ(Anguilla japonica)とオオウナギ(A. marmorata)の河川内分布特性Oral presentation
- 第4回ウナギ学の現状, Mar. 2024, Japanese環境DNA分析を用いたニホンウナギの分布規定要因の推定Oral presentation
- BES Annual Meeting 2023, Dec. 2023, English, British Ecological Society, Belfast, United Kingdom, International conferenceComparison of terrestrial mammal detection methods using environmental DNAPoster presentation
- BES Annual Meeting 2023, Dec. 2023, English, British Ecological Society, Belfast, United Kingdom, International conferenceMethylation analysis of environmental DNA for breeding monitoring of fishPoster presentation
- The 6th Annual Meeting of The eDNA Society, Dec. 2023, JapaneseDevelopment of detection methods for land mammals and birds using environmental DNA[Invited]Nominated symposium
- The 6th Annual Meeting of The eDNA Society, Dec. 2023, JapaneseThe Development and International Standardization of the Manual for Environmental DNA Analysis[Invited]Nominated symposium
- The 6th Annual Meeting of The eDNA Society, Dec. 2023, JapaneseElucidation of the distribution of finless porpoise in Osaka BayPoster presentation
- The 6th Annual Meeting of The eDNA Society, Dec. 2023, JapaneseStudy on the breeding environment of Hynobius setouchiPoster presentation
- The 6th Annual Meeting of The eDNA Society, Dec. 2023, JapaneseDistribution of native and non-native fish species in riversPoster presentation
- The 6th Annual Meeting of The eDNA Society, Dec. 2023, JapaneseDistribution survey of Opisthorchis viverrini and intermediate hosts in Savannakhet Province, Lao PDRPoster presentation
- The 6th Annual Meeting of The eDNA Society, Dec. 2023, JapaneseImplementation of environmental DNA education for high school students and verification of its effectivenessPoster presentation
- The 6th Annual Meeting of The eDNA Society, Dec. 2023, JapaneseEstimation of factors regulating the distribution of Japanese eels in riversPoster presentation
- The 6th Annual Meeting of The eDNA Society, Dec. 2023, JapanesePrediction of spatio-temporal distribution of organisms under climate change using environmental DNA analysis and ecological modelingPoster presentation
- The 6th Annual Meeting of The eDNA Society, Dec. 2023, JapaneseStudy on environmental nucleic acid extraction methods from highly turbid water samplesPoster presentation
- The 6th Annual Meeting of The eDNA Society, Dec. 2023, JapaneseMonitoring Aquatic Plants in Irrigation Ponds Using Environmental DNA Metabarcoding TechniquesPoster presentation
- The 6th Annual Meeting of The eDNA Society, Dec. 2023, JapaneseDevelopment of detection assay for Schistosoma mekongi eDNA and its application in the fieldPoster presentation
- 日本生態学会東北地区会第68回大会, Nov. 2023, Japaneseマリモの過去150年をミジンコで復元するOral presentation
- International Symposium: Past, Present, and Future of the Marine Environment and Ecosystems, Oct. 2023, EnglishProspects of eDNA analysis for macro–organisms: beyond species distribution[Invited]Nominated symposium
- 日本陸水学会第87回大分大会, Oct. 2023, Japanese堆積物DNAを用いた阿寒湖における魚類個体群動態の復元Oral presentation
- 日本陸水学会第87回大分大会, Oct. 2023, Japanese環境DNA分析を用いたニホンウナギのハビタット推定Poster presentation
- 令和5年度日本水産学会秋季大会, Sep. 2023, JapaneseホシガレイVerasper variegatusの環境DNA技術の確立と種苗放流後の分布推定Poster presentation
- 日本哺乳類学会2023年度大会, Sep. 2023, Japanese環境DNA分析を用いた大阪湾におけるスナメリの分布およびホットスポットの解明Poster presentation
- The 26th International Diatom Symposium, Aug. 2023, EnglishThe nuisance invasive species Cymbella janischii in Japan: knowledge from studies and monitoringPoster presentation
- The XXIIIrd International Congress of Genetics, Jul. 2023, EnglishEnvironmental DNA analysis of macroorganisms: recent trend and future prospects in Japan and Worldwide[Invited]Nominated symposium
- Biodiversity Genomics - A Global Perspective: Satellite Meeting of The XXIIIrd International Congress of Genetics, Jul. 2023, EnglishDevelopment of eDNA research in Japan and its application to environmental studies[Invited]Nominated symposium
- The eDNA Society International Meeting 2023, May 2023, EnglishInvestigation of the effect of Artificial Light at Night (ALAN) on wild fish community ~manipulative field experiment and species composition analusis using eDNA methods~Oral presentation
- The eDNA Society International Meeting 2023, May 2023, EnglishApplication of DNA methylation analysis to eDNA studiesOral presentation
- The eDNA Society International Meeting 2023, May 2023, EnglishInvestigation of the potential applicability of eDNA as an environmental education toolPoster presentation
- The eDNA Society International Meeting 2023, May 2023, EnglishHorizontal and vertical distribution of environmental DNA of jack mackerel Trachurus japonicus and Japanese anchovy Engraulis japonicus in Maizuru Bay, Kyoto PrefecturePoster presentation
- The eDNA Society International Meeting 2023, May 2023, EnglishUnraveling the effects of cross rover structures on the habitat of Japanese eelPoster presentation
- The eDNA Society International Meeting 2023, May 2023, EnglishDistribution of finless porpoise (Neophicaena asiaeorientalis) in Osaka Bay, Japan using eDNA analysisPoster presentation
- The eDNA Society International Meeting 2023, May 2023, EnglishSeasonal movement of Rainbow Trout detected by year-round water samplingPoster presentation
- The eDNA Society International Meeting 2023, May 2023, EnglishFish population dynamics in Lake Akan associated with eutrophication reconstructed by sedementary DNAPoster presentation
- The eDNA Society International Meeting 2023, May 2023, EnglishDevelopment of a highly sensitive eDNA detection methodPoster presentation
- The eDNA Society International Meeting 2023, May 2023, EnglishDetection of Southeast Asian liver fluke by multi-region PCR assayPoster presentation
- The eDNA Society International Meeting 2023, May 2023, EnglishRegional-scale effects of deer-induced forest degradation on river ecosystem dynamicsPoster presentation
- さくら湖自然環境フォーラム2022, Mar. 2023, Japanese環境DNA分析で明らかにするさくら湖の魚類相[Invited]Public discourse
- スイゲンゼニタナゴの保全を考えるワークショップ, Mar. 2023, Japanese絶滅危惧種の保全と外来種対策における環境DNA分析の有効性[Invited]Nominated symposium
- 第十八回外来魚情報交換会, Jan. 2023, Japanese環境DNAを用いたスイゲンゼニタナゴと近縁外来種の同時検出系の開発と実践Oral presentation
- 2023年 水生昆虫談話会・日本陸水学会共催シンポジウム, Jan. 2023, Japaneseマクロ生物の環境DNA分析: 生物分布から繁殖期推定まで[Invited]Nominated symposium
- BES Annual Meeting 2022, Dec. 2022, EnglishEnvironmental DNA in lake sediment provides fish information on reconstructing past fauna in lake ecosystemsPoster presentation
- 公開シンポジウム「え、ニジマスって外来種なの？」, Dec. 2022, Japanese水中の生き物調査の新手法：環境DNA[Invited]Nominated symposium
- 第18回棘皮動物研究集会, Dec. 2022, Japaneseクモヒトデ綱(棘皮動物門) の環境DNAメタバーコーディング プライマーの開発Oral presentation
- 環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese棘皮動物における環境DNAメタバーコーディングプライマーの開発Poster presentation
- 環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japaneseクモヒトデ綱（棘皮動物門）の環境DNAメタバーコーディングプライマーの開発Poster presentation
- 環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese各組織が分散してデータを管理し、段階的にデータを共有・公開してデータの価値を高め活用する「データ価値共創環境」の提案Poster presentation
- 環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese環境水からの外来種ミズワタクチビルケイソウの特異的検出Poster presentation
- 環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japaneseスナメリの検出系開発と野外適用Poster presentation
- 環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese河川横断構造物がニホンウナギの河川遡上に与える影響の解明Poster presentation
- 環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese環境DNAにおけるDNAメチル化解析とその生態学的利用に向けたアプローチPoster presentation
- 環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese環境DNAを用いた陸上哺乳類の検出方法の開発Poster presentation
- 環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japaneseバラタナゴ属3種の新しい同時検出系の開発と実用可能性の検討～HRM法、マルチプレックスプローブ法の比較～Poster presentation
- 環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese定量メタバーコーディングを用いた魚類生態調査[Invited]Poster presentation
- 環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese環境DNAメタバーコーディングによる深海無脊椎動物の検出Poster presentation
- The 20th International Congress for Tropical Medicine and Malaria, Oct. 2022, EnglishDetection of Opisthorchis viverrini using environmental DNAPoster presentation
- ELR2022, Sep. 2022, Japaneseダム湖の生物モニタリングに環境DNA技術を適用するためには？[Invited]Nominated symposium
- 日本陸水学会第86回大会, Sep. 2022, Japanese堆積物環境DNAによる琵琶湖魚類の過去復元Oral presentation
- 日本陸水学会第86回大会, Sep. 2022, Japanese夜間の光は魚の生態に影響を及ぼすのか？ー環境DNA技術を用いた種組成調査よりーPoster presentation
- 令和4年度獣医学術中部地区学会, Aug. 2022, Japanese野生動物の糞便からの薬剤耐性大腸菌の分離と性状解析Oral presentation
- 2022年度生物系三学会中国四国地区合同大会, May 2022, Japanese環境DNAと種分布モデルを用いた島根県におけるオオサンショウウオの生息実態の解明Oral presentation
- 2022年度生物系三学会中国四国地区合同大会, May 2022, Japanese環境RNA手法の実用化に向けた検証：ニホンウナギをモデルケースにした野外調査と飼育実験からOral presentation
- 2022年度生物系三学会中国四国地区合同大会, May 2022, Japanese堆積物コアDNAを用いた宍道湖におけるマクロ生物の近過去生息状況の推定Oral presentation
- 令和4年度日本水産学会春季大会, Mar. 2022, Japanese外来種ミズワタクチビルケイソウ検出法の開発Poster presentation
- 2022年日本地理学会春季学術大会, Mar. 2022, JapaneseReconstruction of past Charales algae in Lake Shinji using seda DNA (in Japanese)Oral presentation
- The 69th Annual Meeting of the Ecological Societ of Japan, Mar. 2022, JapaneseSearch for reservoir animals of antimicrobial resistant bacteria using environmental DNA analysisPoster presentation
- The 69th Annual Meeting of the Ecological Societ of Japan, Mar. 2022, JapaneseThe potential of abandoned river as habitats for fish species in Lake BiwaPoster presentation
- The 69th Annual Meeting of the Ecological Societ of Japan, Mar. 2022, JapaneseOptimization of survey design for environmental DNA analysis using a multispecies site occupancy modelPoster presentation
- The 69th Annual Meeting of the Ecological Societ of Japan, Mar. 2022, JapaneseField application of a new eDNA metabarcoding assay using nuclear DNA as a markerPoster presentation
- The 69th Annual Meeting of the Ecological Societ of Japan, Mar. 2022, JapaneseSimultaneous monitoring of the spawning period of multiple fishes using quantitative environmental DNA metabarcodingPoster presentation
- The 69th Annual Meeting of the Ecological Societ of Japan, Mar. 2022, JapaneseInvestigation of an effective method to detect environmental DNA targeting lotic salamander speciesPoster presentation
- The 69th Annual Meeting of the Ecological Societ of Japan, Mar. 2022, JapaneseImpact of the change from a full-circulation to a partial-circulation lake on benthic organismsPoster presentation
- 3rd ESP Asia Conference, Dec. 2021, EnglishReview of integrated research on human well-being, ecosystem services, and spatial characteristics of cities: research trends and future directionsOral presentation
- 令和３年度 魚病症例研究会, Nov. 2021, Japanese環境DNA分析の基礎と魚病を含む感染症への応用[Invited]Keynote oral presentation
- 第24回自然系調査研究機関連絡会議, Japanese大阪府内における環境DNA分析技術を用いた淡水魚分布調査の検討Poster presentation
- The 4th Meeting of The eDNA Society, Nov. 2021, JapaneseNovel universal primers for metabarcoding environmental DNA surveys of cephalopodsInvited oral presentation
- 2021年度水産海洋学会研究発表大会, Nov. 2021, Japanese海洋堆積物のDNA量から魚類個体数の数十年スケール変動を捉えられるか
- The 4th Meeting of The eDNA Society, Nov. 2021, JapaneseImproved accuracy of environmental DNA metabarcoding analysis using thresholds obtained from the defined copy number of DNAPoster presentation
- The 4th Meeting of The eDNA Society, Nov. 2021, JapaneseInvestigation of long-term storage method of environmental DNA glass filter samples at low and room temperaturePoster presentation
- The 4th Meeting of The eDNA Society, Nov. 2021, JapaneseComparison of fish detection results by environmental DNA according to tidal conditions, water sampling volume, and water sampling depth in the coastal waters of Suooshima, Yamaguchi PrefecturePoster presentation
- The 4th Meeting of The eDNA Society, Nov. 2021, JapaneseIs it possible to replace capture surveys? -Impact of primers and DNA databases on macroinvertebrate detection-Poster presentation
- The 4th Meeting of The eDNA Society, Nov. 2021, JapaneseFish eDNA analysis of seawater and sediment at offshore Nobeoka City Miyazaki PrefecturePoster presentation
- The 4th Meeting of The eDNA Society, Nov. 2021, JapaneseAttempt of fish eDNA metabarcoding in a lake with algal bloomsPoster presentation
- The 4th Meeting of The eDNA Society, Nov. 2021, JapaneseDevelopment and evaluation of PCR primers for environmental DNA metabarcoding of AmphibiaPoster presentation
- The 4th Meeting of The eDNA Society, Nov. 2021, JapaneseDevelopment of eDNA metabarcoding assay targeting nuclear DNA as a marker for fishPoster presentation
- The 4th Meeting of The eDNA Society, Nov. 2021, JapaneseSpatio-temporal changes of environmental DNA concentration due to fish spawning activityPoster presentation
- The 4th Meeting of The eDNA Society, Nov. 2021, JapaneseTowards the implementation of environmental DNA metabarcoding for fish surveys in dam reservoirsPoster presentation
- The 4th Meeting of The eDNA Society, Nov. 2021, JapaneseDevelopment of eDNA metabarcoding assays for AnthozoaPoster presentation
- The 34th JSME Meeting, Oct. 2021, EnglishDetection of a group of pathogenic fungi in soils of mycetoma endemic region in the arid area of the Republic of SudanPoster presentation
- 日本生態学会第24回公開講演会 （環境DNAの衝撃：生き物たちの過去・現在・未来を解き明かす）, Mar. 2021, Japanese環境DNA技術を感染症の分布診断に応用する[Invited]Public discourse
- 令和３年度日本水産学会春季大会, Mar. 2021, Japanese産卵と被食に伴う核およびミトコンドリア環境DNAの動態:カタクチイワシとマアジを用いた水槽実験Poster presentation
- 日本生態学会第68回大会, Mar. 2021, Japanese感染症の生態学研究のためのツールとしての環境DNAPoster presentation
- 日本生態学会第68回大会, Mar. 2021, Japanese魚類環境RNAの検出最適化のためのプロトコル検証および野外適用Poster presentation
- 日本生態学会第68回大会, Mar. 2021, Japanese環境核酸分析によるコイの栄養状態の評価Poster presentation
- 日本生態学会第68回大会, Mar. 2021, Japanese核ＤＮＡをマーカーとした魚類環境ＤＮＡメタバーコーディングの新規手法の開発Poster presentation
- 日本生態学会第68回大会, Mar. 2021, Japanese環境DNAを用いたメダカにおける遺伝的撹乱の検出Poster presentation
- 日本生態学会第68回大会, Mar. 2021, Japaneseニホンジカ(Cervus nippon)環境DNAの種特異的検出系の開発Poster presentation
- 日本生態学会第68回大会, Mar. 2021, Japanese環境DNA分析によるオオダイガハラサンショウウオの分布予測Poster presentation
- 日本生態学会第68回大会, Mar. 2021, Japanese環境DNAを用いたバラタナゴ属3種の同時検出系確立と野外適用Poster presentation
- 日本生態学会第68回大会, Mar. 2021, Japanese環境DNAを用いたダム湖における魚類繁殖期の推定Poster presentation
- 日本生態学会第68回大会, Mar. 2021, Japaneseアオコの発生した湖におけるeDNA メタバーコーディング: 中国太湖を事例としてPoster presentation
- 日本生態学会第68回大会, Mar. 2021, Japanese環境DNAとRNAの分解に及ぼす温度とpHの影響Poster presentation
- 日本生態学会第68回大会, Mar. 2021, Japanese琵琶湖の湖底貧酸素化に伴うスジエビ及びイサザへの影響についてPoster presentation
- 日本生態学会第68回大会, Mar. 2021, Japanese鉢虫綱クラゲ類の環境DNAメタバーコーディング系の設計Poster presentation
- 日本生態学会第68回大会, Mar. 2021, Japanese環境DNAメタバーコーディングを用いたトンボ目多様性の評価Poster presentation
- 日本生態学会第68回大会, Mar. 2021, Japanese堆積物環境DNAを用いた琵琶湖における過去生物情報の再構築Poster presentation
- 日本生態学会第68回大会, Mar. 2021, Japaneseダム湖において環境DNAメタバーコーディング手法を用いる際の最適採水地点数の検討Poster presentation
- 日本生態学会第68回大会, Mar. 2021, Japanese長鎖・核DNAおよび海水サンプルを対象とした塩化ベンザルコニウムの環境DNA保存効果Poster presentation
- Kyoto University Center for Ecological Research Joint Research Meeting: New Challenges in Environmental DNA and RNA Research, Dec. 2020, JapaneseUsing environmental nucleic acids in water to understand animal behavior and physiology[Invited]Nominated symposium
- FY2020 Fisheries Resource Conservation Awareness Research Project: Workshop on Environmental DNA Research and Development, Dec. 2020, JapaneseDevelopment and implementation of a survey method for underwater organisms using environmental DNA[Invited]Public discourse
- The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, JapaneseEnvironmental DNA preservation in sediment with application to detecting jellyfish blooms after the tsunamiPoster presentation
- The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, JapaneseDetection of environmental DNA of snakes in paddy fieldsPoster presentation
- The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, JapaneseDetection of spawning behavior of Cyprinidae fish by eDNA analysisPoster presentation
- The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, JapaneseEstimation of the spawning period of Japanese sea cucumber, Apostichopus japonicusPoster presentation
- The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, JapaneseInvestigating an effective sampling method for fish surveys using environmental DNA metabarcodingPoster presentation
- The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, JapaneseState-dependent persistence of environmental DNAPoster presentation
- The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, JapaneseEstimating the spawning period of fish species using environmental DNAPoster presentation
- The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, JapaneseThe influence of marine fish mixed with other species on environmental DNA emissionPoster presentation
- The Joint Meeting of The eDNA Society & The Society of Population Ecology: Ecology in the Age of Big Data & Open Data, Nov. 2020, JapaneseThe effect of body size and activity on eDNA in red-spotted grouper Epinephelus akaaraPoster presentation
- Joint Congress on Global Health 2020 in Osaka, Nov. 2020, JapaneseStudy on intermediate and reservoir hosts of Opisthorchis viverrini in high endemic area in Prey Veng Province, CambodiaPoster presentation
- The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, JapaneseDoes the release of environmental DNA vary with growth stage ? a case study using Japanese giant salamanderPoster presentation
- The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, JavaneseSpecies composition of native freshwater fish and alien Rainbow Trouts in Hokkaido Island using eDNA barcodingPoster presentation
- The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, JapaneseDistribution of smallmouth bass and channel catfish in Yodo River: evaluation using environmental DNAPoster presentation
- The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, JapaneseImproving the accuracy in eDNA analysis using threshold based on known number of DNAPoster presentation
- The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, JapaneseStudy on disease ecology via environmental DNA analysisPoster presentation
- The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, JapaneseEstimating spawning behavior of common carp with environmental RNA analysisPoster presentation
- The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, JapaneseWhat physiological factors does the shedding of environmental DNA associate?Poster presentation
- The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, JapaneseThe ecology and genetic structure of polymorphic migratory behavior of Palaemon paucidens in Lake BiwaPoster presentation
- The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, JapaneseDistribution of Schistosoma environmental DNA in Lake VictoriaPoster presentation
- The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, JapaneseEvaluation of regenerated reed zone as spawning habitat using environmental DNA analysisPoster presentation
- The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, JapaneseSeasonal changes in the structure of the fish community including the endangered loach Parabotia curtus, inferred from environmental DNAPoster presentation
- The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, JapaneseRestoration of past biological information by sedimentary environmental DNA analysis in Lake BiwaPoster presentation
- The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, JapaneseEstimation of reservoir animals of antibiotic resistant bacteria by environmental DNA metabarcoding for mammalsPoster presentation
- The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, JapaneseCan snakes be detected by eDNA analysis?Poster presentation
- The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, JapaneseObservation of eDNA in a breeding season of Siberian salamanderPoster presentation
- The 67th Annual Meeting of the Ecological Society of Japan, Mar. 2020, JapaneseEstimating the breeding period of wild fish using environmental DNAPoster presentation
- Lecture "Biodiversity of coastal seas and restoring environment", Feb. 2020, JapaneseMonitoring biodiversity using environmental DNA[Invited]Public discourse
- JEAS Seminar FY2019, Feb. 2020, JapaneseEnvironmental DNA surveys and its application[Invited]Public discourse
- Meeting of Hokkaido Natural History Rsearch Society 2019, Feb. 2020, JapaneseAn attempt to understand the distribution of native fish communities and rainbow trout in Hokkaido by eDNA metabarcodingOral presentation
- MEC Seminar, Dec. 2019, JapaneseBiological survey using environmental DNA: from the basis to newest researches[Invited]Public discourse
- British Ecological Society Annual Meeting 2019, Dec. 2019, English, International conferenceMonitoring breeding behavior of Japanese giant salamander via eDNA analysisOral presentation
- British Ecological Society Annual Meeting 2019, Dec. 2019, English, International conferenceSedimentary eDNA provides different information on timescale and fish species composition compared with aqueous eDNAPoster presentation
- The 42nd Annual Meeting of the Molecular Biology Society of Japan, Dec. 2019, Japanese, Domestic conferenceParticle size distribution of environmental DNA from fish nuclei and mitochondriaPoster presentation
- The 4th Kuroshio Conference, Nov. 2019, English, Domestic conferenceEnvironmental DNA (eDNA) analysis of pathogenic Leptospira and associated organisms in leptospirosis-endemic Okinawa areas
- The 60th Annual Meeting for the Japanese Society of Tropical Medicine, Nov. 2019, Japanese, Domestic conferenceImprovement of environmental DNA detection of Schistosoma mansoni: comparison of filtration methodsPoster presentation
- The 60th Annual Meeting for the Japanese Society of Tropical Medicine, Nov. 2019, English, Domestic conferenceEnvironmental DNA as a novel tool for clarifying disease ecology[Invited]Nominated symposium
- The 60th Annual Meeting for the Japanese Society of Tropical Medicine, Nov. 2019, English, Domestic conferenceOpisthorchiasis and schistosomiasis from the environmental DNA/GIS perspective[Invited]Nominated symposium
- The 2nd Annual Meeting of The eDNA Society, Nov. 2019, Japanese, Domestic conferenceDynamics of environmental DNA derived from nuclei and mitochondriaNominated symposium
- The 2nd Annual Meeting of The eDNA Society, Nov. 2019, Japanese, Domestic conferenceImprovement of environmental DNA detection method for Schistosoma mansoniPoster presentation
- The 2nd Annual Meeting of The eDNA Society, Nov. 2019, Japanese, Domestic conferenceObservation of small amphibians in wetland using environmental DNAPoster presentation
- The 2nd Annual Meeting of The eDNA Society, Nov. 2019, Japanese, Domestic conferenceVertical distribution of fish environmental DNA in a dam reservoirPoster presentation
- The 2nd Annual Meeting of The eDNA Society, Nov. 2019, Japanese, Domestic conferenceElucidation of the life history of Palaemon paucidens in Lake BiwaPoster presentation
- The 2nd Annual Meeting of The eDNA Society, Nov. 2019, Japanese, Domestic conferenceElucidation of seasonal movement of Acheilognathus longipinnis using environmental DNA and relationship with environmental characteristicsPoster presentation
- The 2nd Annual Meeting of The eDNA Society, Sep. 2019, Japanese, Domestic conferenceChanges of the concentration of environmental DNA of fish before and after flooding-a case study in a lentic water-Poster presentation
- The 23rd Annual Meeting of the Ecology and Civil Engineering Society, Sep. 2019, Japanese, Domestic conferenceToward the implementation of fish monitoring using environmental DNA[Invited]Nominated symposium
- The 84th Annual Meeting of the Japanese Society of Limnology, Sep. 2019, English, Domestic conferenceApplication of bamboo biomass resources in agrochemical-free rice farming: effects on Odonata diversityOral presentation
- The 84th Annual Meeting of the Japanese Society of Limnology, Sep. 2019, Japanese, Domestic conferenceSearch for a rare freshwater fish, Acheilognathus typus, and related organisms through environmental DNA metabarcodingOral presentation
- 2019 Autumn Meeting of Japanese Society of Fisheries Science, Sep. 2019, Japanese, Domestic conferenceWater temperature and biomass dependence of nucleus and mitochondria environmental DNA of Japanese jack mackerelOral presentation
- Japan Society of Civil Engineers 2019 Annual Meeting, Sep. 2019, Japanese, Kagawa University, Takamatsu, Japan, Domestic conferenceOn the efficacy of eDNA metabarcoding for a basin scale fish surveyOral presentation
- Japan Society of Civil Engineers 2019 Annual Meeting, Sep. 2019, Japanese, Kagawa University, Takamatsu, Japan, Domestic conferenceOn the detection accuracy of fish species in Koidegawa river by the difference in sampling volume and sampling positionOral presentation
- 21st Annual Meeting of the Society of Evolutionary Studies, Japan, Aug. 2019, Japanese, Hokkaido University, Sapporo, Japan, Domestic conferenceThe applicability of sedimentary environmental DNA analysis for restoration of past biological information[Invited]Nominated symposium
- 21st Annual Meeting of the Society of Evolutionary Studies, Japan, Aug. 2019, Japanese, Hokkaido University, Sapporo, Japan, Domestic conferenceEstimating population abundance using environmental DNA[Invited]Nominated symposium
- 21st Annual Meeting of the Society of Evolutionary Studies, Japan, Aug. 2019, Japanese, Hokkaido University, Sapporo, Japan, Domestic conferenceEnvironmental DNA for the ecology of infectious diseases[Invited]Nominated symposium
- Fisheries Society of the British Isles, Symposium 2019, Jul. 2019, English, University of Hull (Hull City, United Kingdom), International conferenceDistributional pattern of black sea bream Acanthopagrus schlegelii estimated from environmental DNA: seasonal changes in marine and river watersOral presentation
- Fisheries Society of the British Isles, Symposium 2019, Jul. 2019, English, University of Hull (Hull City, United Kingdom), International conferenceBiomass-dependent emission of environmental DNA in jack mackerel Trachurus japonicus juvenilesPoster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceDetection of eDNA of alien ant speciesfrom soil samplesPoster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceDetection of common carp using eRNA from water samplesPoster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceApplication of environmental DNA method in tropical wetland: example of Schistosoma mansoni in Lake Victoria, KenyaPoster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceImprovement of eDNA extraction method from sedimental samplesPoster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceEstablishment of eDNA metabarcoding method for aquatic beetlesPoster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceEstimation of mammal communities using environmental DNA from water and soil samplesPoster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceDetection and quantification of macroorganisms using species-specific environmental DNA analysis[Invited]Nominated symposium
- FY2018 Institute of Human Development Symposium, Mar. 2019, Japanese, Kobe University, Kobe, Japan, Domestic conferenceEnvironmental DNA as a tool for citizen science[Invited]Nominated symposium
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceEstimation of the source of environmental DNA by gene-specific environmental RNA analysis focusing on tissue-specific messenger RNA expressionsPoster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceUsing environmental DNA to detect the spatial and temporal distribution of Palaemonpaucidens in Lake BiwaPoster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceHabitat identification of Hynobius kimurae in Rokko mountains using environmental DNA analysisOral presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceAn attempt to quantify of invasive fishusing environmental DNA analysisPoster presentation
- 2019 Meeting of Kansai Organization for Nature Conservation, Mar. 2019, Japanese, Tasaka Museum of Natural History, Osaka, Japan, Domestic conferenceAn attempt for the distribution survey of Japanese giant salamander in Shiga Prefecture, JapanPoster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceEnvironmental DNA reveals the relationships among land uses and freshwater fish fauna around Mts. RokkoPoster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceCapturing spawning migration of the endemic anadromous species, Shishamo smelt (Spirinchus lanceolatus), using environmental DNAPoster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceEstimation of eDNA shedding and degradation rates of Japanese jack mackerel using nuclear DNA markerPoster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceEvaluating the effectiveness of control measures for invasive red-eared slider turtles using environmental DNAPoster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceAmphibians environmental DNA detection using metabarcoding analysisPoster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceEstablishment of environmental DNA detection system of Salamandrella keyserlingii and its application in Kushiro MarshPoster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceCan breeding behavior of Japanese giant salamander detect just by water?~Based on the results of the environmental DNA survey in artificial nests~Poster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, English, Kobe Convention Center, Kobe, Japan, Domestic conferenceVisualizing environmental DNA using cytogeneticsOral presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, Japanese, Kobe Convention Center, Kobe, Japan, Domestic conferenceThe use of SNP markers to detect genetic variation in environmental DNAPoster presentation
- The 66th Annual Meeting of th Ecological Society of Japan, Mar. 2019, English, Kobe Convention Center, Kobe, Japan, Domestic conferenceApplication of bamboo biomass resources in agrochemical-free rice farming: effects onodonate diversityPoster presentation
- 2019 Academic Meeting of Tottori Prefectural Institute of Public Health and Environmental Science, Feb. 2019, Japanese, Tottori University, Tottori, Japan, Domestic conferenceMonitoring of aquatic organisms using environmental DNAOral presentation
- 64th Seminar of Japan Society on Water Environment, Jan. 2019, Japanese, Jidousya-Kaikan, Tokyo, Japan, Domestic conferenceDistribution survey for rare or invasive species by species-specific detection of eDNAPublic discourse
- Symposium of Marine Biodiversity Research Area of CREST program, Jan. 2019, Japanese, Sasagawa Peace Foundation Building, Tokyo, Japan, Domestic conferenceOn the development of eDNA analysis and basic techniquesPublic discourse
- Lecture for High School Teachers in Osaka, Dec. 2018, Japanese, Viale Osaka, Osaka, Japan, Domestic conferenceLooking into the world of aquatic organisms: environmental DNAPublic discourse
- Kickoff Symposium of eDNA Research Center, Yamaguchi University, Dec. 2018, Japanese, International Hotel Ube, Ube, Japan, Domestic conferenceApplicability of eDNA analysis: estimation of biomass and multispecies-detection[Invited]Invited oral presentation
- British Ecological Society Annual Meeting 2018, Dec. 2018, English, Birmingham ICC, Birmingham, UK, International conferenceSpatio-temporal detection patterns of fish eDNA in lentic ecosystemsPoster presentation
- British Ecological Society Annual Meeting 2018, Dec. 2018, English, Birmingham ICC, Birmingham, UK, International conferenceHigh water temperature and fish biomass can accelerate the shedding and degradation of the Japanese jack mackerel (Trachurus japonicus) environmental DNAPoster presentation
- Joint International Triopical Medicine Meeting 2018, Dec. 2018, English, Amari Watergate Bangkok, Bangkok, Thailand, International conferenceEnvironmental DNA: a different approach for food/water borne helminths studiesOral presentation
- British Ecological Society Annual Meeting 2018, Dec. 2018, English, Birmingham ICC, Birmingham, UK, International conferenceDetection of zebrafish messenger RNAs from tank water: implications for the practicability of environmental RNA analysis for aquatic macroorganismsPoster presentation
- Kinki Meeting of the Institution of Professional Engineers, Japan, Nov. 2018, Japanese, Urbanex Bingo-cho, Osaka, Japan, Domestic conferenceAquatic survey by water collection: introduction of eDNA techniquesPublic discourse
- The 41st Annual Meeting of Molecular Bilogy Society of Japan, Nov. 2018, Japanese, Pacifico Yokohama, Yokohama, Japan, Domestic conferenceEnvironmental DNA: from micro- to macro-organisms[Invited]Invited oral presentation
- Kobe City Symposium on Biodiversity, Nov. 2018, Japanese, Ozi Zoo, Kobe, Japan, Domestic conferenceEnvironmental DNA: a new method of monitoring underwater organismsPublic discourse
- Ecology and Civil Engineering Society Sendai Branch Symposium, Nov. 2018, Japanese, Mahara, Miharu, Fukushima, Japan, Domestic conferenceFish monitoring in dam lakes by using environmental DNAPoster presentation
- Japanese Society of Fisheries Oceanography Symposium, Nov. 2018, Japanese, The University of Tokyo, Kashiwa, Chiba, Japan, Domestic conferenceApplication of eDNA analysis to fish distribution survey[Invited]Invited oral presentation
- The 83rd Meeting of theJapanese Society of Limnology, Oct. 2018, Japanese, Okayama University, Okayama, Japan, Domestic conferenceImprovement of sedimentary environmental DNA extraction method and prospect for restoration of past faunaOral presentation
- Green Science Seminar at Seishin Women's High School, Oct. 2018, Japanese, Seishin Women's High School, Domestic conferenceUnderwater biodiversity survey using environmental DNAPublic discourse
- 2018 Annual Meeting of the Ichthyological Society of Japan, Oct. 2018, Japanese, National Olympics Memorial Youth Center, Tokyo, Japan, Domestic conferenceDetection of genetic diversity using environmental DNA analysisOral presentation
- 2018 Annual Meeting of the Ichthyological Society of Japan, Oct. 2018, Japanese, National Olympics Memorial Youth Center, Tokyo, Japan, Domestic conferenceDetection of genetic disturbance by colored medaka using environmental DNA methodOral presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceVerification of environmental DNA detection accuracy of Acheilognathus longipinnis in Kiso RiverPoster presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceEstimation of spatiotemporal distribution and migration timing of common shrimp in Lake BiwaPoster presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceMonitoring of distribution of Ayu fish and cold water disease bacteria in Nagara and Ibi RiversPoster presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceDetection of environmental DNA from bites of herbivorous insectsPoster presentation
- 2018 Open Seminar of JAES Kansai Branch, Sep. 2018, Japanese, Osaka Garden Palace, Osaka, Japan, Domestic conferenceOn the basis of environmental DNA analysis and distribution survey for rare or alien speciesPublic discourse
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceEstimation of the breeding season of the Japanese giant salamander using environmental DNA analysisPoster presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceAttempt to investigate the ecology of fish and shellfish living in Lakes Shinji and Nakaumi using environmental DNA analysisPoster presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceEstimating fish population abundance or biomass by integrating quantitative environmental DNA analysis and hydrodymamic modelingPoster presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceProtocol for environmental DNA surveyPublic discourse
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceDetecting spawning migration of endemic anadromous species, Shishamo smelt (Spirinchus lanceolatus), using environmental DNAPoster presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferencePossibility of biomass estimation of coastal fish via environmental DNA[Invited]Invited oral presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceRelationship between rare insect species and invasive alien species in tameike ponds clarified with environmental DNA analysisPoster presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceComparison of detection trends with fish faunal survey by eDNA metabarcoding and literature data of rivers around Lake BiwaPoster presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceFreshwater fish fauna around Rokko Montains clarified by environmental DNA metabarcodingPoster presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceEffect of water temperature and biomass on release and decomposition of environmental DNAPoster presentation
- Shiga Water Environment Business Promotion Forum: Research and Technology Subcommittee, Sep. 2018, Japanese, Piazza Omi, Otsu, Japan, Domestic conferenceIntroduction to environmental DNAPublic discourse
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceDistribution pattern of Japanese black seabream inferred from environmental DNAPoster presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceDevelopment of environmental DNA metabarcoding detection assay for dragonfly speciesPoster presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceOptimization of eDNA sampling in dam resoiversPoster presentation
- The 22nd Annual Meeting of the Ecology and Civil Engineering Society, Sep. 2018, Japanese, Tokyo Institute of Technology, Tokyo, Japan, Domestic conferenceInvestigation of inhibitory factors of environmental DNA detection of turtle speciesPoster presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceInvestigation of inhibitory factors of environmental DNA detection of turtlePoster presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, English, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceEvaluation of the fish distribution and their environmental DNA in a semi-closed bay using a three-dimensional tracer modelPoster presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, English, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceEnvironmental DNA metabarcoding reveals fish species in Qingdao Underwater WorldPoster presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, English, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceAn optional cost-effective method of extracting environmental DNA from filter membranes in large scale eDNA surveysPoster presentation
- The First Annual Meeting of the eDNA Society, Sep. 2018, Japanese, National Museum of Emerging Science and Innovation, Tokyo, Japan, Domestic conferenceDetection of amphbiam species by using 16SrRNA metabarcoding of environmental DNAPoster presentation
- 2018 Hyogo prefecture freshwater fishermen workshop, Aug. 2018, Japanese, Hyogo Prefecture Kanzaki Branch Office, Domestic conferenceForensic investigation of rivers? examining underwater fauna with environmental DNAPublic discourse
- 2018 Open Seminar of JAES Hokkaido Branch, Aug. 2018, Japanese, Sapporo Education and Culture Hall, Sapporo, Japan, Domestic conferenceThe present and future situation of environmental DNA studiesPublic discourse
- 2018 ESA Annual Meeting, Aug. 2018, English, Ernest N. Morial Convention Center, New Orleans, Louisiana, USA, International conferenceThe use of SNP markers in environmental DNA to detect intraspecific genetic variationPoster presentation
- 2018 ESA Annual Meeting, Aug. 2018, English, Ernest N. Morial Convention Center, New Orleans, Louisiana, USA, International conferenceExamining PCR protocol for amplifying whole mitochondrial DNA of fish from environmental DNAPoster presentation
- Science Gallery 2018, Jul. 2018, Japanese, International House, Osaka, Japan, Domestic conferenceFuturistic glasses looking into the world of aquatic organisms? ~ Environmental DNAPublic discourse
- Seminar on environment in Mukogawa River, Jun. 2018, Japanese, Kobe City Hall, Domestic conferenceClarifying underwater organisms by using environtal DNAPublic discourse
- ASLO 2018 Summer Meeting, Jun. 2018, English, Victoria Conference Centre, British Columbia, Canada, International conferenceMaximizing the potential of environmental DNA metabarcoding for fish detection in lentic ecosystemPoster presentation
- The 8th East Asian Federation of Ecological Societies International Congress, Apr. 2018, English, Nagoya University, Nagoya, Japan, International conferenceDetection of genetic diversity of a fish population using environmental DNA metabarcoding: Analysis strategies for eliminating sequence artifactsPoster presentation
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conferenceCan Salvelinus species coexist with rainbow trout in Hokkaido? ~Based on distribution data by environmental DNA ~Poster presentation
- 2018 Spring Meeting of Japanese Society of Fisheries Science, Mar. 2018, Japanese, Tokyo University of Marine Science and Technology, Domestic conferenceHorizontal and vertical distribution of environmental DNA of Trachurus japonicus and Engraulis japonicus in Maizuru BayOral presentation
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conferenceGenetic structure of common shrimp in Lake Biwa related to the life history polymorphismPoster presentation
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conferenceInvestigation of basic technologies for environmental RNA analysisPoster presentation
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conferenceDetection of breeding behavior of common carp using environmental RNAPoster presentation
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conferenceImprovement of environmental DNA detection assay for South East Asian liver flukePoster presentation
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conferenceCurrent status and prospects of basic researches on environmental DNA analysis[Invited]Nominated symposium
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conferenceDetection of genetic diversity using environmental DNA Analysis: Application to wild Ayu population and its detectabilityOral presentation
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conferenceFactors regulating biodiversity of ponds - Case studies on environmental DNA analysis of dragonfly and fish species-Poster presentation
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conferenceEstablishment of environmental DNA assays for freshwater turtlesPoster presentation
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conferenceEstimation of breeding season of the Japanese Giant Salamander using environmental DNA analysisPoster presentation
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conferenceFixed sites monitoring of Hida salamander using environmental DNAPoster presentation
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conferenceEstimation of amphibian distribution by environmental DNA and analysis of environmental factorsPoster presentation
- 2018 Spring Meeting of Japanese Society of Fisheries Science, Mar. 2018, Japanese, Tokyo University of Marine Science and Technology, Tokyo, Japan, Domestic conferenceCoastal dependence of Acanthopagrus schlegelii indestigated by environmental DNA and distribution characteristics of egg-laying and larval stageOral presentation
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conferenceExploring the dynamics of host and pathogenic organisms using environmental DNA[Invited]Nominated symposium
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conferenceAttempt to estimate resources of Corbicula japonica in Lake Shinji using environmental DNAOral presentation
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conferenceInvestigation of fish fauna around Mt. Rokko using environmental DNA metabarcodingOral presentation
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conferenceIdentifying breeding seasons and locations using environmental DNA[Invited]Nominated symposium
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, Japanese, Sapporo Convention Center, Sapporo, Japan, Domestic conferenceInvestigation of PCR conditions for acquisition of full length mitochondrial DNA sequence from environmental DNA samplesPoster presentation
- The 55th Leptospira Symposium, Mar. 2018, Japanese, Kyushu University, Fukuoka, Japan, Domestic conferenceA research on environmental DNA of Leptospira in north OkinawaOral presentation
- The 65th Annual Meeting of Ecological Society of Japan, Mar. 2018, English, Sapporo Convention Center, Sapporo, Japan, International conferenceThe impact of biodiversity in river on valuation of urban ecosystem services: the case of Hanshin region using eDNA and the residents' life satisfaction dataOral presentation
- 11th International Symposium Exploring the Global Sustainability, Mar. 2018, English, Graduate School of Human Development and Environment, International conferenceEnvironmental DNA: next generation monitoring method for aquatic biodiversity[Invited]Invited oral presentation
- Symposium "Considering the freshwater organisms in Okayama: mainly on turtles", Feb. 2018, Japanese, Okayama University of Science, Domestic conferenceSuvey for turtles using environmental DNA[Invited]Invited oral presentation
- Green Science Seminar at Seishin Women's High School, Jan. 2018, Japanese, Seishin Women's High School, Domestic conferenceUnderwater biodiversity survey using environmental DNAPublic discourse
- Ecology Across Borders: Joint Annual Meeting 2017, Dec. 2017, English, ICC Ghent, Belgium, International conferenceIdentifying a breeding habitat of a critically endangered fish, Acheilognathus typus, by combining eDNA and conventional surveysPoster presentation
- Ecology Across Borders: Joint Annual Meeting 2017, Dec. 2017, English, ICC Ghent, Belgium, International conferenceFish larvae inside? Indirect assessment of fish larvae presence in host mussels using environmental DNA analysis.Poster presentation
- Technical Seminar, Society of Environmental Conservation Engineering, Nov. 2017, Japanese, Osaka Kyoiku University, Osaka, Japan, Domestic conferenceMonitoring of rare species using environmental DNA analysisPublic discourse
- Global Health 2017, Nov. 2017, Japanese, University of Tokyo, Tokyo, Japan, Domestic conferenceSoutheast Asian liver fluke in Cambodia: identification of a new endemic areaOral presentation
- The 149th RIHN Seminar "Observation, analysis and theory in ecology for next generations -What we have achieved in global environment studies-", Nov. 2017, English, Research Institute for Humanity and Nature, Kyoto, Japan, International conferenceDevelopment of distribution survey method for macro-organisms using environmental DNA[Invited]Invited oral presentation
- The 33rd Annual Meeting of Society for Population Ecology, Oct. 2017, English, Kyusyu University, Fukuoka, Japan, Domestic conferenceThe application of eDNA technique for the monitoring of marine fish speciesPoster presentation
- The 82nd Annual Meeting of the Japanese Society of Limnology, Sep. 2017, Japanese, Komagatake Grand Hotel, Senboku, Akita, Japan, Domestic conferenceIdentification of a breeding habitat of a critically endangered fish species, Acheilognathus typus, in the main stream of the Omono RiverOral presentation
- The 82nd Annual Meeting of the Japanese Society of Limnology, Sep. 2017, Japanese, Komagatake Grand Hotel, Senboku, Akita, Japan, Domestic conferenceApplication of environmental DNA analysis in the Lake Biwa and connected lakes[Invited]Nominated symposium
- ELR2017 Nagoya, Sep. 2017, Japanese, Nagoya University, Nagoya, Japan, Domestic conferenceRelationship between the coverage of Egeria densa and eDNA concentration in Saba and Takatsu riversPoster presentation
- 2017 Annual Meeting of Japanese Society of Civil Engineers, Sep. 2017, Japanese, Kyushu University, Fukuoka, Japan, Domestic conferenceConfirmation of the effectiveness of eDNA analysis for the survey of rare species - detection of an unknown breeding site of Acheilognathus typusOral presentation
- The 82nd Annual Meeting of Japanese Society of Limnology, Sep. 2017, Japanese, Komagatake Grand Hotel, Senboku, Akita, Japan, Domestic conferenceWhat is environmental DNA analysis?Public discourse
- 2017 Annual Meeting of Japanese Society of Civil Engineers, Sep. 2017, Japanese, Kyushu University, Fukuoka, Japan, Domestic conferenceMonitoring method for Acheilognathus longipinnis using environmental DNAOral presentation
- ELR2017 Nagoya, Sep. 2017, Japanese, Nagoya University, Nagoya, Japan, Domestic conferenceEnvironmental DNA survey of alien-species distribution in ponds - application for Trachemys scripta and consideration of PCR inhibitionPoster presentation
- The 82nd Annual Meeting of the Japanese Society of Limnology, Sep. 2017, Japanese, Komagatake Grand Hotel, Senboku, Akita, Japan, Domestic conferenceEnvironmental DNA analysis using nuclear DNA markersOral presentation
- ELR2017 Nagoya, Sep. 2017, Japanese, Nagoya University, Nagoya, Japan, Domestic conferenceAre environmental DNA methods effective for species survey in rivers and reservoirs?[Invited]Nominated symposium
- The 82nd Annual Meeting of the Japanese Society of Limnology, Sep. 2017, Japanese, Komagatake Grand Hotel, Senboku, Akita, Japan, Domestic conferenceComparison of the accuracy of visual and environmental DNA surveys for the turtle species, Trachemys scripta: Toward constructing a potential map for prediction of invasion into pondsPoster presentation
- The 11th Helminth Research Meeting, Sep. 2017, English, Iojima, Nagasaki, Japan, Domestic conferenceEnvironmental DNA and its application in opisthorchiasis and schistosomiasis in endemic areasOral presentation
- 2017 ESA Annual Meeting, Aug. 2017, English, Oregon Convention Center, Portland, Oregon, U.S.A., International conferenceQuantitative monitoring of multispecies fish environmental DNA using high-throughput sequencingOral presentation
- 2017 ESA Annual Meeting, Aug. 2017, English, Oregon Convention Center, Portland, Oregon, U.S.A., International conferenceLonger DNA fragments enables the improved estimation of distribution and biomass using environmental DNAPoster presentation
- 2017 ESA Annual Meeting, Aug. 2017, English, Oregon Convention Center, Portland, Oregon, U.S.A., International conferenceEnvironmental DNA survey methods: Water sampling methods using an unmanned aerial vehiclePoster presentation
- International Congress of Ecology 2017, Aug. 2017, English, China National Convention Center, Beijing, China, International conferenceEnvironmental DNA reflects the spatial and temporal distribution of common prawn, Palaemon paucidens, in Lake BiwaPoster presentation
- Invitation letter to the near future - messages from nice step researcher 2016, Jul. 2017, Japanese, Japanese Ministry of Education, Culture, Sports, Science and Technology (Tokyo, Japan), Domestic conferenceBiological survey by only collecting water: Development of environmental DNA technology to understand the type and quantity of aquatic organismsPublic discourse
- Let's learn! 'Sea Science'!, Jul. 2017, Japanese, Kobe City Industry Promotion Center (Kobe, Japan), Domestic conferenceInvestigate the fish in the Kobe Port using "environmental DNA"!Public discourse
- Special Seminar at HYOGO KANKYO TAIKENKAN, Jul. 2017, Japanese, HYOGO KANKYO TAIKENKAN（Sayo Town, Hyogo, Japan), Domestic conferenceInvestigating underwater organisms using DNAPublic discourse
- The 47th Symposium on the Sub-arctic Fisheries and Oceanography, Jun. 2017, Japanese, Hokkaido University, Sapporo, Japan, Domestic conferenceWhat is environmental DNA?[Invited]Invited oral presentation
- 2017 International Workshop on Environmental Genomics, Jun. 2017, English, St. Johns, Canada, International conferenceSpecies-specific detection of vertebrates and invertebrates by eDNA analysis[Invited]Invited oral presentation
- The Japan Society for Analytical Chemistry, 77th Meeting, May 2017, Japanese, Ryukuko University, Kyoto, Japan, Domestic conferenceSearch for habitats of threatened species using environmental DNA[Invited]Invited oral presentation
- The 67th Hiyoshi Seminar, May 2017, Japanese, Hiyoshi Co. LTD., Ohmi-Hachiman, Shiga, Japan, Domestic conferenceOn the survey methods for endangered or alien species using environmental DNA[Invited]Invited oral presentation
- 2017 The Korean Society of Fisheries and Aquatic Sciences Spring Meeting, May 2017, English, Korea, International conferenceNumerical simulation on distribution of environmental DNA of Japanese jack mackerel (Trachurus japonicus) in a semi-closed bayOral presentation
- The 202nd Yomiuri Techno Forum, Apr. 2017, Japanese, Nippon Press Center, Tokyo, Japan, Domestic conferenceOrganism is found by examining the water ~the wonders of environmental DNA~Public discourse
- The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conferenceRediscovery of a critically endangered freshwater fish;Acheilognathus typus;in the main stream of Omono RiverPoster presentation
- The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conferenceDetection of environment DNA of rare fish species in rivers in Sasayama City;Hyogo PrefecturePoster presentation
- 2017 Annual Meeting of the Japanese Society of Fisheries Science, Mar. 2017, Japanese, Tokyo University of Marine Science and Technology, Tokyo, Japan, Domestic conferenceDetection of non-migrating ecotype of common shrimp in Lake Biwa ~ Analysis using environmental DNAPoster presentation
- The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conferenceQuantitative monitoring of environmental DNA from multiple fish species by using mass-sequencingOral presentation
- 2017 Annual Meeting of the Japanese Society of Fisheries Science, Mar. 2017, Japanese, Tokyo University of Marine Science and Technology, Tokyo, Japan, Domestic conferenceDetection of environmental DNA of Japanese jack mackerel from sediments: aquarium experiments and field surveysOral presentation
- The 122nd Meeting of Japanese Society of Animal Science, Mar. 2017, Japanese, Kobe University, Kobe, Japan, Domestic conferenceBiological survey methods using environmental DNA information[Invited]Invited oral presentation
- The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conferenceEvaluation of a species distribution model based on environmental DNA analysis - An case study of Japanese giant salamander (Andrial japonicus) -Oral presentation
- The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conferenceProgress of the biodiversity survey by environmental DNA analysis and its applicability[Invited]Nominated symposium
- The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conferenceApplication of environmental DNA method to the ecology of infectious diseases: detection of schistosomesPoster presentation
- The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conferenceEvaluation of intraspecific genetic variation using environmental DNA: a new method for removing false haplotypes derived from NGS analysisOral presentation
- 2017 Annual Meeting of the Japanese Society of Fisheries Science, Mar. 2017, Japanese, Tokyo University of Marine Science and Technology, Tokyo, Japan, Domestic conferenceDevelopment of jellyfish control measure using environmental DNA: seasonal monitoring and detection from polypOral presentation
- The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conferenceEnvironments DNA meta barcoding reveals seasonal change of fish community in a riverOral presentation
- The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conferenceFish fauna survey by environmental DNA metabarcoding - a case study of small rivers in Rokko Mountains -Poster presentation
- The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conferenceEffect of water temperature on degradation and size fraction of environmental DNAPoster presentation
- The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conferenceGenetic structure analysis of hybrid populations using environmental DNA analysis with nuclear DNA markersOral presentation
- 2017 Annual Meeting of the Japanese Society of Fisheries Science, Mar. 2017, Japanese, Tokyo University of Marine Science and Technology, Domestic conferenceRelationship between population density and environmental DNA concentration of Lateolabrax japonicus fry in different habitatsOral presentation
- The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conferenceEnvironment DNA detection of Hynobius spp. using universal primersPoster presentation
- The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conferenceOptimization of environmental DNA detection method in dam reservoirsPoster presentation
- The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conferenceInvestigation of optimal biomass estimation method using environmental DNA using Hemigrammocypris rasborella as a model speciesPoster presentation
- The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, English, Waseda University, Tokyo, Japan, Domestic conferenceEnvironmental DNA as an ecological tool for salmonid fish distributionOral presentation
- The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, Japanese, Waseda University, Tokyo, Japan, Domestic conferenceSpatio-temporal distribution of common prawn in Lake Biwa clarified by eDNA analysisPoster presentation
- The 64th Annual Meeting of Ecological Society of Japan, Mar. 2017, English, Waseda University, Tokyo, Japan, Domestic conferenceA large scale study of aquatic plants according to eDNA analysisOral presentation
- The 38st Green Science Seminar, Jan. 2017, Japanese, Fukuyama University, Fukuyama, Japan, Domestic conferenceDevelopment of a novel survey method for aquatic organisms using environmental DNAPublic discourse
- 2016 Natural Science Lecture, Dec. 2016, Japanese, Agenda 21, Citizen's Environmental Conference in Toyonaka, Toyonaka City Central Community Center, Toyonaka, Osaka, Japan, Domestic conferenceA new biological survey method "environmental DNA analysis"Public discourse
- 2016 Annual Meeting of Japanese Society of Fisheries Oceanography, Nov. 2016, Japanese, Tokyo University of Marine Science and Technology, Domestic conferenceNumerical experiment on environmental DNA distribution ofJapanese jack mackerel in Maizuru BayOral presentation
- The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conferenceDetection of a semi-aquatic mammal nutria by environmental DNA analysisPoster presentation
- The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conferenceMetabarcoding of fish DNA using environmental DNA in sedimentsOral presentation
- The 57th Annual Meeting for the Japanese Society of Tropical Medicine, Nov. 2016, Japanese, Hitotsubashi University, Tokyo, Japan, Domestic conferenceDetection of Opisthorchis viverrini DNA from Environmental Water Sample by eDNA MethodsPoster presentation
- The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conferenceCurrent status and issues of environmental DNA analysisOral presentation
- The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conferenceThe use of SNP markers in environmental DNA researchesOral presentation
- The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conferenceHabitat survey of Acheilognathus longipinnis in Yodo River using environmental DNAOral presentation
- The 10th Nagoya presentation meeting of the River Foundation, Nov. 2016, Japanese, Wink Aichi, Nagoya, Japan, Domestic conferenceA novel ecosystem survey method using environmental DNA and its applicability to water environment management[Invited]Invited oral presentation
- The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conferenceDistribution survey of mountain stream inhabiting amphibians Onychodactylus japonicus using environmental DNAOral presentation
- The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conferenceEvaluation of the species distribution model for Japanese giant salamander based on environmental DNA analysisOral presentation
- The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conferenceEstimation of fish distribution and biomass: comparison of real-time PCR and digital PCROral presentation
- The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conferenceEnvironmental DNA detection of Hynobisu species using universal primersOral presentation
- The 81st Annual Meeting of Japanese Society of Limnology, Nov. 2016, Japanese, Ryukyu University (Okinawa, Japan), Domestic conferenceHabitat survey for Trachemys scripta: application of environmental DNA technology and examination for PCR inhibition factorOral presentation
- The 5th Japan-Taiwan Ecology Workshop, Nov. 2016, English, Ryukoku University, Kyoto, Japan, International conferenceEnvironmental DNA anlysis: introduction and some examples of target species detection[Invited]Invited oral presentation
- The 10th National Health Research Forum, Laos, Oct. 2016, English, Savannakhet, Laos, International conferenceApplication of environmental DNA to detect Opisthorchis viverrini in water sample in Savhannakhet, LaosPoster presentation
- Annual Meeting of Japan Society of Civil Engineers, Sep. 2016, Japanese, Sendai City, Miyagi, Japan, Domestic conferenceEstimating the distribution of Acheilognathus longipinnis in Yodo River by eDNA anakysisOral presentation
- 2016 Annual Meeting of The Ichthyological Society Japan, Sep. 2016, Japanese, Gifu City, Gifu, Japan (Gifu University), Domestic conferenceTrial of monitoring of natural monument, Acheilognathus longipinnis using eDNAPoster presentation
- Ecological Society of America Annual Meeting 2016, Aug. 2016, English, Fort Lauderdale, Florida, U.S.A., International conferenceYear-round migration history of ayu sweetfish assessed by environmental DNA analysis: Implications for conservation and fisheries managementPoster presentation
- Ecological Society of America Annual Meeting 2016, Aug. 2016, English, Fort Lauderdale, Florida, U.S.A., International conferenceUse of environmental DNA to survey the distributions of the salamander and fish speciesPoster presentation
- 24th Molecular Parasitology Workshop / 14th Molecular Parasitology and Malaria Research Forum, Aug. 2016, English, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan, Domestic conferenceUse of Environmental DNA for detection of fish-water borne zoonosis and perspectives to the application of the technology in MadagascarOral presentation
- Ecological Society of America Annual Meeting 2016, Aug. 2016, English, Fort Lauderdale, Florida, U.S.A., International conferenceEstimating the distribution of the Japanese giant salamander (Andrias japonicus) by ecological niche modeling based on environmental DNA detectionPoster presentation
- Ecological Society of America Annual Meeting 2016, Aug. 2016, English, Fort Lauderdale, Florida, U.S.A., International conferenceEnvironmental DNA method for estimating fish abundance and biomassOral presentation
- Ecological Society of America Annual Meeting 2016, Aug. 2016, English, Fort Lauderdale, Florida, U.S.A., International conferenceApplication of environmental DNA to analysis of mitochondrial haplotypes of fishPoster presentation
- International symposium on alternative stable states: a unifying concept in global change ecology, Jul. 2016, English, Kyoto University, Kyoto, Japan, International conferenceEnvironmental DNA analysis provides "snapshots" of species distribution[Invited]Invited oral presentation
- International Symposium of Probiotics Application on Aquaculture, Jul. 2016, English, National Kaohsiung Marine University, Kaohsiung, Taiwan, International conferenceApplication of environmental DNA analysis to ecological surveys[Invited]Invited oral presentation
- 35th Special Lecture of Hyogo Biotechnology Study Group, Jun. 2016, Japanese, Kobe University, Kobe, Japan, Domestic conferenceUnderstanding the distribution of underwater species using environmental DNA[Invited]Invited oral presentation
- Kyoto Sangyo University BioForum, Jun. 2016, Japanese, Kyoto Sangyo University, Kyoto, Japan, Domestic conferenceUnderwater biodiversity revealed by environmental DNA analysisPublic discourse
- The 26th lecture on animal survey methods for environmental assessments, Jun. 2016, Japanese, Osaka, Japan, Domestic conferenceEnvironmental DNAPublic discourse
- 2016 Korean Society of Oceanology Spring Meeting, May 2016, English, Busan, Korea, Domestic conferenceAnalysis on relationship between fish distribution and environmental DNA in a semi -closed bay using a three dimensional regional circulation modelOral presentation
- The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conferenceEnvironmental DNA detection from sedimentsPoster presentation
- The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conferenceA large survey of Japanese giant salamander (Andrias japonicus) by using environmental DNAPoster presentation
- The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conferenceDetection of mitochondria DNA haplotype in ayu (Plecoglossus altivelis) by environmental DNA analysis[Invited]Nominated symposium
- The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conferenceWhat kind of information to be obtained by the environmental DNA analysis?[Invited]Nominated symposium
- The 85th Annual Meeting of the Japanese Society of Parasitology, Mar. 2016, Japanese, Miyazaki Citizen's Plaza, Miyazaki, Japan, Domestic conferenceDetection of liver fluke Opisthorchis viverrini by environmental DNA analysisOral presentation
- The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conferenceApplication of environmental DNA analysis for ecology of infectious diseases: the detection of liver fluke Opisthorchis viverrini[Invited]Nominated symposium
- The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conferencePractices and questions of environmental DNA research: challenges for the frontier of ecology[Invited]Nominated symposium
- The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conferenceApplication of environmental DNA analysis for sub-merged plants: tests of survey methods and evaluation of the usefulnessPoster presentation
- The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conferenceLarge scale fish fauna survey in the rivers using "MiFish", the universal primers for environmental DNA analysisPoster presentation
- The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conferenceBiomass estimation of ayu (Peloglossus altivelis) by environmental DNA analysis in the logic environments[Invited]Nominated symposium
- The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conferenceFish fauna monitoring in Yodo River by using environmental DNA metabarcodingPoster presentation
- The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conferenceThe difference of degradation rate between different lengths of environmental DNAPoster presentation
- The 2016 Spring Meeting of Japanese Society of Fisheries Science, Mar. 2016, Japanese, Tokyo University of Marine Science and Technology, Tokyo, Japan, Domestic conferenceVerification of the environmental DNA dispersion process in a coastal areaOral presentation
- The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conferenceEnvironmental DNA survey using multiplex PCR for endemic and exotic fish species in the ponds in Hyogo Prefecture[Invited]Nominated symposium
- The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conferenceEstimation of the distribution and biomass by digital PCR[Invited]Nominated symposium
- The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conferenceDetection of environmental DNA of Hynobius species using universal primersPoster presentation
- The 63rd Annual Meeting of the Ecological Society of Japan, Mar. 2016, Japanese, Sendai International Center, Sendai, Japan, Domestic conferenceEstimation of genotype frequencies by quantitative SNP analysis[Invited]Nominated symposium
- 2015 Annual Meeting British Ecological Society, Dec. 2015, English, Edinburgh, UK., International conferenceSpatial and temporal distribution surveys for marine jellyfish using environmental DNAPoster presentation
- 2015 Annual Meeting British Ecological Society, Dec. 2015, English, Edinburgh, UK, International conferenceeDNA based non-invasive method to analyze mitochondrial haplotype of fishPoster presentation
- 日本陸水学会第80回大会, Sep. 2015, Japanese, 北海道函館市, Domestic conference新たな環境DNAマーカーとしての核DNAの利用Oral presentation
- 日本土木学会第70回年次学術講演会, Sep. 2015, Japanese, 岡山県岡山市(岡山大学), Domestic conference環境影響評価における新しい調査手法の試み- 環境DNAを用いたニホンザリガニ生息場の推定-Oral presentation
- 日本土木学会第70回年次学術講演会, Sep. 2015, Japanese, 岡山県岡山市(岡山大学), Domestic conference環境影響評価における新しい調査手法の試み-環境DNAを用いたイタセンパラ生息の推定-Oral presentation
- 日本陸水学会第80回大会, Sep. 2015, Japanese, 北海道函館市, Domestic conference環境DNA分析によるコイの季節移動の推定Oral presentation
- 日本陸水学会第80回大会, Sep. 2015, Japanese, 北海道函館市, Domestic conference環境DNA手法の新展開:魚類個体群の遺伝的多様性評価の試みPoster presentation
- 日本陸水学会第80回大会, Sep. 2015, Japanese, 北海道函館市, Domestic conferenceデジタルPCRを用いた環境DNAによる生物量・生物分布推定Poster presentation
- 日本陸水学会第80回大会, Sep. 2015, Japanese, 北海道函館市, Domestic conferenceため池の環境DNA量と生物量の比較:池干し時の採捕調査による検証Oral presentation
- 第30回環境計量技術事例発表会, Jul. 2015, Japanese, 兵庫県計量協会環境計量証明部会, Domestic conference水を汲むだけで生物分布がわかる？環境DNA 手法の開発と実践Public discourse
- 2015 Society of Wetland Scientist Annual Meeting, Jun. 2015, English, Providence, Rhode Island, USA, International conferenceEnvironmental DNA Assessment of Rare Endemic Species and Closely Related Exotic Species: A Case Study of Giant Salamanders in Japan[Invited]Nominated symposium
- 日本生態学会第62回全国大会, Mar. 2015, Japanese, 鹿児島県鹿児島市, Domestic conference環境DNA分析を用いた希少在来種と近縁外来種の流域スケール調査：京都府桂川のオオサンショウウオを例にOral presentation
- 日本生態学会第62回全国大会, Mar. 2015, Japanese, 鹿児島県鹿児島市, Domestic conference環境DNA手法を用いた在来および外来コイの生息地利用の推定Oral presentation
- 日本生態学会第62回全国大会, Mar. 2015, Japanese, 鹿児島県鹿児島市, Domestic conference環境DNA手法の新たな展開〜沈水植物の検出法の開発〜Poster presentation
- 日本生態学会第62回全国大会, Mar. 2015, Japanese, Domestic conference環境DNA技術を利用した沈水植物の生物量推定Oral presentation
- 日本陸水学会近畿支部会第26回研究発表会, Mar. 2015, Japanese, 京都府京都市, Domestic conference環境DNA技術を用いた，ため池の生物分布調査：池干しによる採捕調査との比較Oral presentation
- 日本海洋学会 2015年度春季大会 沿岸海洋シンポジウム「沿岸海洋学における観測研究の最前線I」, Mar. 2015, Japanese, 東京都港区, Domestic conference環境DNAを用いた魚類モニタリング[Invited]Nominated symposium
- 日本生態学会第62回全国大会, Mar. 2015, Japanese, 鹿児島県鹿児島市, Domestic conference環境DNAを用いたクラゲ類の分布調査～舞鶴湾のアカクラゲを例に～Poster presentation
- 日本生態学会第62回全国大会, Mar. 2015, Japanese, 鹿児島県鹿児島市, Domestic conference環境DNAの正体を探る：挙動の分析と観察によるアプローチPoster presentation
- 日本生態学会第62回全国大会, Mar. 2015, Japanese, 鹿児島県鹿児島市, Domestic conference環境DNAとマルチプレックスPCRを用いた複数魚種の同時検出Poster presentation
- 日本生態学会第62回全国大会, Mar. 2015, Japanese, 鹿児島県鹿児島市, Domestic conferenceeDNA解析による舞鶴湾におけるマアジ資源量の推定Poster presentation
- Joint 2014 Annual Meeting British Ecological Society and Société Française d’Ecologie, Dec. 2014, English, Lille, France, International conferenceAssessment of the effect of artificial obstructions on fish migration in a river using environmental DNAOral presentation
- Joint 2014 Annual Meeting British Ecological Society and Société Française d’Ecologie, Dec. 2014, English, Lille, France, International conferenceA basin-scale application of environmental DNA assessment for rare endemic and exotic giant salamander species in JapanPoster presentation
- 2014年度日本魚類学会年会, Nov. 2014, Japanese, 神奈川県小田原市, Domestic conference魚類環境DNA用ユニバーサルプライマーの開発と次世代シーケンサを用いた分析法の確立Oral presentation
- PAWEES 2014 International Conference, Oct. 2014, English, Kaohsiung, Taiwan, International conferenceDevelopment of a method for detecting inhabitation of the Dojo loach using environmental DNAOral presentation
- 日本陸水学会第79回大会, Sep. 2014, Japanese, 茨城県つくば市, Domestic conference集団における外来遺伝子頻度を定量する環境DNA手法の開発と野外への適用Oral presentation
- 日本陸水学会第79回大会, Sep. 2014, Japanese, 茨城県つくば市, Domestic conference環境DNA手法の希少生物種調査への応用: 兵庫県下のため池におけるカワバタモロコの分布調査Oral presentation
- 日本陸水学会第79回大会, Sep. 2014, Japanese, 茨城県つくば市, Domestic conferenceため池の動物プランクトン群集の規定要因:生産性,生態系サイズ,捕食者に着目した解析Poster presentation
- Evolutionary Community Ecology 2014, Sep. 2014, English, Kyoto, International conferenceCommunity ecology using environmental DNA[Invited]Invited oral presentation
- 平成26年度農業農村工学会大会講演会, Aug. 2014, Japanese, 新潟県新潟市, Domestic conference水から抽出したDNAを用いて生息魚類を検出する方法の試みOral presentation
- EcoHealth2014 (The 5th Biennial Conference of the International Association for Ecology & Health), Aug. 2014, English, Montréal, Canada, International conferenceThe Japanese Society for Ecology and Health: J-EcoHealth - An IntroductionPoster presentation
- 2014 ESA Annual Meeting, Aug. 2014, English, Sacramento, CA, USA, International conferenceMonitoring upstream migration of fish using environmental DNA: Towards a more efficient method for assessing habitat connectivityPoster presentation
- 2014 ESA Annual Meeting, Aug. 2014, English, Sacramento, CA, USA, International conferenceMarine fish surveys using environmental DNAPoster presentation
- EcoHealth2014 (The 5th Biennial Conference of the International Association for Ecology & Health), Aug. 2014, English, Montréal, Canada, International conferenceImpact of expansion of wet rice fields on the risk of liver fluke infection in Lao P.D.R.Poster presentation
- 14th SCA Annual Conferenc, Jun. 2014, English, Kuala Lumpur, Malaysia, International conferenceWhy probability literacy is important to resolve framing conflicts in environmental issues?Oral presentation
- 日本生態学会第61回全国大会, Mar. 2014, Japanese, 広島県広島市, Domestic conference同種内外来種の侵入規模の迅速把握[Invited]Nominated symposium
- 日本生態学会第61回全国大会, Mar. 2014, Japanese, 広島県広島市, Domestic conference水生生物の移動分散モニタリングへの環境DNA技術の適用：流水環境における研究例と展望[Invited]Nominated symposium
- 日本生態学会第61回全国大会, Mar. 2014, Japanese, Domestic conference環境DNAとフェノロジー研究: 現状と未来[Invited]Nominated symposium
- 日本生態学会第61回全国大会, Mar. 2014, Japanese, 広島県広島市, Domestic conference核DNAをマーカーとした環境DNA解析[Invited]Nominated symposium
- 日本生態学会第61回全国大会, Mar. 2014, Japanese, 広島県広島市, Domestic conferenceオオクチバス等の外来魚モニタリングにおける環境DNA技術の有用性の検証－調査手法の違いによる結果の比較を通して―[Invited]Nominated symposium
- Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing, Mar. 2014, English, Higashi-Hiroshima City, Japan, International conferenceLarge-scale environmental DNA assessment for Japanese and Chinese giant salamanders in Katsura River, JapanPoster presentation
- The 61st Annual Meeting of the Ecological Society of Japan, Mar. 2014, English, Hiroshima City, Japan, Domestic conferenceEnvironmental DNA release velocity from different sized fishPoster presentation
- 第25回龍谷大学新春技術講演会, Jan. 2014, Japanese, 滋賀県大津市, Domestic conference環境DNA分析による生物の分布および生物量の推定技術Poster presentation
- 第25回龍谷大学新春技術講演会, Jan. 2014, Japanese, 滋賀県大津市, Domestic conference環境DNA技術で多様性の湖に挑むPoster presentation
- 夢・化学21 高校生のための化学講座（浜松）, Dec. 2013, Japanese, 静岡大学（静岡県浜松市）, Domestic conference見えない生き物を見つけ出す環境DNA技術Public discourse
- 第29回個体群生態学会大会, Oct. 2013, Japanese, 大阪府堺市, Domestic conference環境DNAを用いた水中生物モニタリングの現状[Invited]Nominated symposium
- 日本陸水学会第78回大会, Sep. 2013, Japanese, 滋賀県大津市, Domestic conference環境DNAを利用した同種内外来種の迅速把握Poster presentation
- 日本陸水学会第78回大会, Sep. 2013, Japanese, 滋賀県大津市, Domestic conference環境DNAを用いた在来および外来オオサンショウウオの検出Oral presentation
- 第8回科学コミュニケーション研究会年次大会, Sep. 2013, Japanese, 京都大学, Domestic conferenceSTIに向けた政策プロセスへの関心層別関与フレーム設計Poster presentation
- 中国環境問題研究拠点 国際シンポジウム「東アジアにおける都市化と福祉・環境問題—上海を中心に—」, Jul. 2013, Japanese, 総合地球環境学研究所 中国環境問題研究拠点, 総合地球環境学研究所, International conference上海における環境意識に関する質問票調査[Invited]Invited oral presentation
- 26th International Congress for Conservation Biology (ICCB 2013), Jul. 2013, English, Baltimore, MD, USA, International conferenceUsing environmental DNA to estimate the distributions and biomass of fish[Invited]Nominated symposium
- サイエンス・カフェひょうごin佐用, Mar. 2013, Japanese, 兵庫県佐用町, Domestic conference川の水をはかれば生き物がわかる？～環境DNA研究の最先端～Public discourse
- 日本生態学会第60回大会, Mar. 2013, Japanese, 日本生態学会, 静岡県静岡市, Domestic conference先端技術（環境DNA，次世代シーケンス）を使って生態学を変えていくには？[Invited]Nominated symposium
- 日本生態学会第60回大会, Mar. 2013, Japanese, 日本生態学会, 静岡県静岡市, Domestic conference環境DNA分析の実用化：野外で使える前処理法の検討Poster presentation
- 日本生態学会第60回大会, Mar. 2013, Japanese, 日本生態学会, 静岡県静岡市, Domestic conference環境DNA技術を用いた外来種モニタリング手法の開発Oral presentation
- 日本生態学会第60回大会, Mar. 2013, Japanese, 日本生態学会, 静岡県静岡市, Domestic conference環境DNAを用いてタイ肝吸虫の生態を探るPoster presentation
- 日本生態学会第60回大会, Mar. 2013, Japanese, 日本生態学会, 静岡県静岡市, Domestic conference環境DNAを用いた魚類の在不在判定による遡河行動のモニタリングOral presentation
- 日本生態学会第60回大会, Mar. 2013, Japanese, 日本生態学会, 静岡県静岡市, Domestic conferenceメコン中流域の魚類の移動と寄生虫感染率の変化Poster presentation
- 第二回同位体環境学シンポジウム, Feb. 2013, Japanese, 京都府京都市, Domestic conference環境DNAを用いた微生物マッピング：コイヘルペスウイルスを例にNominated symposium
- 国際シンポジウム「湖の現状と未来可能性」, Jan. 2013, Japanese, 中国上海市, International conference環境改変と感染症―琵琶湖のコイヘルペスウイルス病を例に―Nominated symposium
- The 9th International Conference on Environmental, Cultural, Economic and Social Sustainability, Jan. 2013, English, Hiroshima City, Japan, International conferenceDetection and quantification of fish presence and biomass using environmental DNA to monitor population sustainabilityPoster presentation
- EcoHealth2012, Oct. 2012, English, Kunming City, China, International conferenceMonitoring of fish pathogenic viruses in freshwater environmentsPoster presentation
- 日本陸水学会第77回大会, Sep. 2012, Japanese, 日本陸水学会, 名古屋大学, Domestic conference環境水中のウィルス定量：水域生態系での感染症研究への適用[Invited]Invited oral presentation
- 日本陸水学会第77回大会, Sep. 2012, Japanese, 日本陸水学会, 愛知県名古屋市, Domestic conference環境DNAを用いた魚類相の定性的把握法[Invited]Invited oral presentation
- 日本陸水学会第77回大会, Sep. 2012, Japanese, 日本陸水学会, 名古屋大学, Domestic conference核酸の動態から感染症の生態を探る[Invited]Invited oral presentation
- 日本陸水学会第77回大会, Sep. 2012, Japanese, 日本陸水学会, 愛知県名古屋市, Domestic conferenceため池の水生動物モニタリングに環境DNAを応用する[Invited]Invited oral presentation
- 2012 ASLO Aquatic Sciences Meeting, Jul. 2012, English, ASLO, Otsu City, Shiga, Japan, International conferenceTransmission dynamics of an emerging pathogen Cyprinid herpesvirus 3 and its impact on host population genetic structureOral presentation
- 2012 ASLO Aquatic Sciences Meeting, Jul. 2012, English, ASLO, Otsu City, Shiga, Japan, International conferenceSeasonal and spatial distribution of Cyprinid herpesvirus 3 in water and sediment of a lagoon of Lake Biwa, JapanPoster presentation
- 2012 ASLO Aquatic Sciences Meeting, Jul. 2012, English, ASLO, Otsu City, Shiga, Japan, International conferenceDetection and quantification of fish presence/biomass in ponds using environmental DNAPoster presentation
- 日本生態学会第59回大会／第５回東アジア生態学会連合大会合同大会, Mar. 2012, Japanese, 日本生態学会, EAFES, 滋賀県大津市, Domestic conference新興病原体コイヘルペスウイルスの野生宿主個体群における持続性Oral presentation
- 日本生態学会第59回大会／第５回東アジア生態学会連合大会合同大会, Mar. 2012, Japanese, 日本生態学会, EAFES, 滋賀県大津市, Domestic conference湖水中に溶存するDNA断片から魚類のバイオマスを推定するOral presentation
- Joint Meeting of The 59th Annual Meeting of ESJ and The 5th EAFES International Congress, Mar. 2012, English, 日本生態学会, EAFES, Otsu City, Shiga, Japan, International conferenceSeasonal and spatial distribution of Cyprinid herpesvirus 1 and Cyprinid herpesvirus 2 in Lake Biwa, JapanOral presentation
- Joint Meeting of The 59th Annual Meeting of ESJ and The 5th EAFES International Congress, Mar. 2012, English, Otsu Ciyu, Shiga, Japan, International conferenceDynamics of koi herpesvirus and related factors in freshwater environments (EAFES Symposium ES09: Interactions of pathogen-host with environments)Nominated symposium
- 感染症若手フォーラム2012, Feb. 2012, Japanese, 長崎県長崎市, Domestic conference感染症の生態学的解析：コイヘルペスウイルス病を例にOral presentation
- 第10回 地球研地域連携セミナー「水辺の保全と琵琶湖の未来可能性」, Jan. 2012, Japanese, 滋賀県大津市, Domestic conference環境の変化と魚の病気Public discourse
- The 6th RIHN International Symposium, Oct. 2011, English, Kyoto City, Japan, International conferenceKoi herpesvirus disease as a model of environmental diseaseNominated symposium
- Emergence of Viral Diseases: Ecology and Evolution of Koi Herpes Virus, Jul. 2011, English, Muenster, Germany, International conferenceKHV dynamics in natural freshwater environments.Nominated symposium
- 4th Congress of European Microbiologists, FEMS 2011, Jun. 2011, English, FEMS, Geneva City, Switzerland, International conferenceDynamics of Cyprinid herpesvirus 3 in natural environments in JapanPoster presentation
- 日本生態学会第58回大会, Mar. 2011, Japanese, 日本生態学会, 北海道札幌市, Domestic conference水温の変動パタンが魚類の生理コストに与える影響についてPoster presentation
- 日本生態学会第58回大会, Mar. 2011, Japanese, 日本生態学会, 北海道札幌市, Domestic conference環境DNAを用いた魚類相把握法の開発Oral presentation
- 日本応用動物昆虫学会第55回大会, Mar. 2011, Japanese, 日本応用動物昆虫学会, 福岡県福岡市, Domestic conferenceコイヘルペスウイルス（KHV）病の発症率はストレスの影響を受けるか？Oral presentation
- 日本生態学会第58回大会, Mar. 2011, Japanese, 日本生態学会, 北海道札幌市, Domestic conferenceコイヘルペスウイルス病が琵琶湖の野生型コイへもたらした影響Oral presentation
- 日本生態学会第58回大会, Mar. 2011, Japanese, 日本生態学会, 北海道札幌市, Domestic conferenceコイにとっての岸辺環境の有用性とストレス回避のトレードオフPoster presentation
- 日本陸水学会第75回大会, Sep. 2010, Japanese, 日本陸水学会, 青森県弘前市, Domestic conference中国雲单省Erhai湖における水温の時空間分布パターンPoster presentation
- 日本陸水学会第75回大会, Sep. 2010, Japanese, 日本陸水学会, 青森県弘前市, Domestic conference全国の自然河川におけるコイヘルペスウイルスの分布Oral presentation
- 平成22年度 日本動物学会中部地区大会公開シンポジウム, Jul. 2010, Japanese, 日本動物学会中部支部会, 岐阜県岐阜市, Domestic conference魚類の多様性と視物質の分子進化Public discourse
- Workshop on the Linkage between CyHV-3 (KHV) and Humans, May 2010, English, Jerusalem, Israel, International conferenceSeasonal dynamics of CyHV-3 in natural freshwater environmentsOral presentation
- Workshop on the Linkage between CyHV-3 (KHV) and Humans, May 2010, English, Jerusalem, Israel, International conferenceDetection of cyprinid herpesvirus-3 (CyHV-3) in environmental water and sedimentsOral presentation
- 日本生態学会第57回大会, Mar. 2010, Japanese, 日本生態学会, 東京都目黒区, Domestic conference堆積物におけるコイヘルペスウイルスの検出・定量Oral presentation
- 第44回日本水環境学会年会, Mar. 2010, Japanese, 日本水環境学会, 福岡県福岡市, Domestic conference環境水中の低密度ウイルスに対する濃縮システムの開発Oral presentation
- 日本生態学会第57回大会, Mar. 2010, English, 日本生態学会, 東京都目黒区, Domestic conferenceコイヘルペスウイルス感染症と人間の相互作用環Nominated symposium
- 日本生態学会第57回大会, Mar. 2010, Japanese, 日本生態学会, 東京都目黒区, Domestic conferenceコイの行動性体温調節と環境水温の時空間的不均一性がコイヘルペスウイルス症の蔓延に与える影響についてOral presentation
- 日本陸水学会第74回大会, Sep. 2009, Japanese, 日本陸水学会, 大分県大分市, Domestic conference野外におけるコイの行動性体温調節とその季節変化：コイヘルペスウイルス病蔓延時期との対応についてOral presentation
- 日本陸水学会第74回大会, Sep. 2009, Japanese, 日本陸水学会, 大分県大分市, Domestic conference琵琶湖におけるコイヘルペスウイルスの動態解析Oral presentation
- The 5th International Tunicate Meeting, Jun. 2009, English, Naha City, Okinawa, Japan, International conferenceMicroarray analysis of circadian gene expressions in Ciona intestinalisPoster presentation
- Workshop on CyHV-3 disease in an environment-human linkage, Apr. 2009, English, Kyoto City, Japan, International conferenceSeasonal distribution of cyprinid herpesvirus 3 in Lake BiwaOral presentation
- 日本生態学会第56回大会, Mar. 2009, Japanese, 日本生態学会, 岩手県盛岡市, Domestic conference琵琶湖におけるコイヘルペスウイルスの分布と季節変化Oral presentation
- 日本生態学会第56回大会, Mar. 2009, Japanese, 日本生態学会, 岩手県盛岡市, Domestic conference湖岸形態と水温分布：魚類への影響の考察Poster presentation
- 第42回日本水環境学会大会, Mar. 2009, Japanese, 日本水環境学会, 愛知県名古屋市, Domestic conferenceウイルス状粒子の迅速濃縮法とKHVの検出Oral presentation
- 第15回日本時間生物学会学術大会, Nov. 2008, Japanese, 日本時間生物学会, 岡山県岡山市, Domestic conferenceカタユウレイボヤの概日リズムPoster presentation
- 日本陸水学会第73回大会, Oct. 2008, Japanese, 日本陸水学会, 北海道札幌市, Domestic conference環境水中に存在するコイヘルペスウイルスの定量Oral presentation
- 12th International Conference on Integrated Diffuse Pollution Management (IWA DIPCON 2008), Aug. 2008, English, Khon Kaen City, Thailand, International conferenceDevelopment of a Rapid Concentration System for Virus in Environmental WaterOral presentation
- International Symposium on Environmental Change, Pathogens, and Human Linkages, Jun. 2008, English, Kyoto City; Japan, International conferenceRelationship of lake morphometry and shore configuration to the temperature distribution in lagoons, and implications for its effect on fish healthPoster presentation
- International Symposium on Environmental Change, Pathogens, and Human Linkages, Jun. 2008, English, Kyoto City; Japan, International conferenceQuantification method of Koi herpesvirus (KHV) in environmental water using cation-coated filter method and external standard virusPoster presentation
- International Symposium on Environmental Change, Pathogens, and Human Linkages, Jun. 2008, English, Kyoto City, Japan, International conferenceInternational Symposium on Environmental Change, Pathogens, and Human LinkagesOral presentation
- International Symposium on Environmental Change, Pathogens, and Human Linkages, Jun. 2008, English, Kyoto City; Japan, International conferenceDevelopment of an on site rapid concentration system for virus in environmental waterPoster presentation
- International Symposium on Environmental Change, Pathogens, and Human Linkages, Jun. 2008, English, Kyoto City; Japan, International conferenceDetection of koi herpesvirus DNA from natural river waterPoster presentation
- 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会, Dec. 2007, Japanese, 日本分子生物学会, 日本生化学会, 神奈川県横浜市, Domestic conferenceカタユウレイボヤのcDNAマイクロアレイの数理解析による時計候補遺伝子の抽出Poster presentation
- 第13回日本時間生物学会学術大会, Nov. 2006, Japanese, 日本時間生物学会, 東京都千代田区, Domestic conferenceカタユウレイボヤ（Ciona intestinalis）の概日リズムPoster presentation
- 日本動物学会第77回大会, Sep. 2006, Japanese, 日本動物学会, 島根県松江市, Domestic conferenceカタユウレイボヤ（Ciona intestinalis）における概日振動遺伝子群の探索Oral presentation
- 第12回日本時間生物学会大会, Nov. 2005, Japanese, 日本時間生物学会, 茨城県つくば市, Domestic conferenceカタユウレイボヤ（Ciona intestinalis）の概日振動遺伝子群Poster presentation
- 日本動物学会第75回大会, Sep. 2004, Japanese, 日本動物学会, 神戸市灘区, Domestic conferenceニホンミツバチにおける新たなperiod遺伝子 mRNAバリアントの解析Poster presentation
- 日本動物学会第74回大会, Sep. 2003, Japanese, 日本動物学会, 北海道函館市, Domestic conferenceニホンミツバチ及び膜翅目昆虫におけるperiod遺伝子mRNAバリアントの解析Oral presentation
- 日本動物学会第73回大会, Sep. 2002, Japanese, 日本動物学会, 石川県金沢市, Domestic conferenceアユの錐体視物質遺伝子に関する研究Oral presentation
- The 1st Asian Conference on Photobiology, Jun. 2002, English, Higashiura Town, Hyogo, Japan, International conferenceStudies of opsin genes in a smelt fish, Ayu (Plecoglossus altivelis).Poster presentation
- 日本動物学会第72回大会, Oct. 2001, Japanese, 日本動物学会, 福岡県福岡市, Domestic conferenceアユのVAオプシンに関する研究Oral presentation
- 第65回日本陸水学会大会, Sep. 2000, Japanese, 日本陸水学会, 福岡県福岡市, Domestic conferenceバイカル湖における水中光の分光放射特性Oral presentation
- 日本動物学会第70回大会, Sep. 1999, Japanese, 日本動物学会, 山形県山形市, Domestic conferenceアユの視物質遺伝子に関する研究（２）Oral presentation
- 日本動物学会第68回大会, Oct. 1997, Japanese, 日本動物学会, 奈良県奈良市, Domestic conference魚類の視物質及びチトクロームb遺伝子の分子進化Oral presentation