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INOUE Kunio
Graduate School of Science / Division of Biology
Professor

Researcher basic information

■ Research Areas
  • Life sciences / Developmental biology
  • Life sciences / Molecular biology

Research activity information

■ Paper
  • Saori Tani-Matsuhana, Yuga Kawata, Kunio Inoue
    Last, Elsevier BV, Mar. 2023, Developmental Biology, 495, 1 - 7, English, International magazine
    [Refereed]
    Scientific journal

  • Takashi Miwa, Keigo Ohtani, Kunio Inoue, Hiroshi Sakamoto
    Wiley, Oct. 2022, Genes to Cells, 27(10) (10), 621 - 628, English, International magazine
    [Refereed]
    Scientific journal

  • Kazuhiro Fukumura, Rei Yoshimoto, Luca Sperotto, Hyun-Seo Kang, Tetsuro Hirose, Kunio Inoue, Michael Sattler, Akila Mayeda
    AbstractHuman pre-mRNA introns vary in size from under fifty to over a million nucleotides. We searched for essential factors involved in the splicing of human short introns by screening siRNAs against 154 human nuclear proteins. The splicing activity was assayed with a model HNRNPH1 pre-mRNA containing short 56-nucleotide intron. We identify a known alternative splicing regulator SPF45 (RBM17) as a constitutive splicing factor that is required to splice out this 56-nt intron. Whole-transcriptome sequencing of SPF45-deficient cells reveals that SPF45 is essential in the efficient splicing of many short introns. To initiate the spliceosome assembly on a short intron with the truncated poly-pyrimidine tract, the U2AF-homology motif (UHM) of SPF45 competes out that of U2AF65 (U2AF2) for binding to the UHM-ligand motif (ULM) of the U2 snRNP protein SF3b155 (SF3B1). We propose that splicing in a distinct subset of human short introns depends on SPF45 but not U2AF heterodimer.
    Springer Science and Business Media LLC, Dec. 2021, Nature Communications, 12(1) (1), 4910 - 4910, English, International magazine, Co-authored internationally
    [Refereed]
    Scientific journal

  • Saori Tani-Matsuhana, Kunio Inoue
    Last, Elsevier BV, Sep. 2021, Cells & Development, 167, 203725, English
    [Refereed]
    Scientific journal

  • Yuichiro Mishima, Kunio Inoue
    Springer US, Feb. 2021, Methods in Molecular Biology, 347 - 354
    [Invited]
    In book

  • Hiroki Fukuchi, Yukinobu Hayashida, Kunio Inoue, Yoshifusa Sadamura
    Forkhead box (FOX) proteins constitute a family of transcription factors that are evolutionarily conserved in various species ranging from yeast to humans. These proteins have functions during development as well as in adulthood. To date, many reports have described the functions of FOX family genes in cancer cells, but the role of FOXB2 is not well understood. In one of the pancreas ductal adenocarcinoma cell lines, Panc-1 cells, we showed here that FOXB2 expression is barely detectable and that CpG islands in the 5' regions of the FOXB2 are highly methylated. These findings led us to hypothesize that FOXB2 acts as a tumor suppressor. To clarify our hypotheses, we investigated the effects of FOXB2 over-expression in Panc-1 cells. We obtained FOXB2 stable transfectants, and these clones exhibited reduced spheroid formation ability. Expression of β-catenin, which is reported to be over-expressed in various cancer cells, was highly suppressed in FOXB2 stable transfectants. Moreover, side population (SP) cell fractions, which have a high tumorigenesis and metastatic potential, as well as anchorage-independent growth ability, were reduced. These results suggest that FOXB2 has the ability to inhibit the malignant characteristics of Panc-1 in vitro.
    Oct. 2019, Genes to Cells, 24(10) (10), 674 - 681, English, International magazine
    [Refereed]
    Scientific journal

  • Takashi Miwa, Kunio Inoue, Hiroshi Sakamoto
    In Caenorhabditis elegans, germline cells remain transcriptionally silenced during embryogenesis. The transcriptional silencing is achieved by two different mechanisms: One is the inhibition of RNA polymerase II in P2-P4 cells at the establishment stage, and another is chromatin-based silencing in two primordial germ cells (PGCs) at the maintenance stage; however, the molecular mechanism underlying chromatin-based silencing is less understood. We investigated the role of the chromodomain protein MRG-1, which is an essential maternal factor for germline development, in transcriptional silencing in PGCs. PGCs lacking maternal MRG-1 showed increased levels of two histone modifications (H3K4me2 and H4K16ac), which are epigenetic markers for active transcription, and precocious activation of germline promoters. Loss of MES-4, a H3K36 methyltransferase, also caused similar derepression of the germline genes in PGCs, suggesting that both MRG-1 and MES-4 function in chromatin-based silencing in PGCs. In addition, the mrg-1 null mutant showed abnormal chromosome structures and a decrease in homologous recombinase RAD-51 foci in PGCs, but the mes-4 null mutant did not show such phenotypes. Taken together, we propose that MRG-1 has two distinct functions: chromatin-based transcriptional silencing and preserving genomic integrity at the maintenance stage of PGCs.
    May 2019, Genes to Cells, 24(5) (5), 377 - 389, English, International magazine
    [Refereed]
    Scientific journal

  • Tani-Matsuhana, S, Vieceli, F. M, Gandhi, S, Inoue, K, Bronner, M. E
    Dec. 2018, Developmental Biology, 444(1) (1), S209 - S218, English
    [Refereed]
    Scientific journal

  • Saori Tani-Matsuhana, Rie Kusakabe, Kunio Inoue
    Last, Sep. 2018, Development Genes and Evolution, 228(5) (5), 189 - 196, English
    [Refereed]
    Scientific journal

  • Splicing activator RNPS1 suppresses errors in pre-mRNA splicing: A key factor for mRNA quality control.
    Fukumura, A, Inoue, K, Mayeda, A
    Feb. 2018, Biochem. Biophys. Res. Commun., 496, 921 - 926, English
    [Refereed]
    Scientific journal

  • Manami Kobayashi, Saori Tani-Matsuhana, Yasuka Ohkawa, Hiroshi Sakamoto, Kunio Inoue
    Germline and somatic cell distinction is regulated through a combination of microRNA and germ cell specific RNA-binding proteins in zebrafish. An RNA-binding protein, DND, has been reported to relieve the miR-430-mediated repression of some germ plasm mRNAs such as nanos3 and tdrd7 in primordial germ cells (PGCs). Here, we showed that miR-430-mediated repression is not counteracted by the overexpression of DND protein in somatic cells. Using a) lambda N-box B tethering assay in the embryo, we found that tethering of DND to reporter mRNA results in translation repression without affecting mRNA stability. Translation repression by DND was not dependent on another germline-specific translation repressor, Nanos3, in zebrafish embryos. Moreover, our data suggested that DND represses translation of nanog and dnd mRNAs, whereas an RNA-binding protein DAZ-like (DAZL) promotes dnd mRNA translation. Thus, our study showed that DND protein functions as a translation repressor of specific mRNAs to control PGC development in zebrafish. (C) 2017 Elsevier Inc. All rights reserved.
    ACADEMIC PRESS INC ELSEVIER SCIENCE, Mar. 2017, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 484(2) (2), 235 - 240, English
    [Refereed]
    Scientific journal

  • The Exon Junction Complex controls the efficient and faithful splicing of a subset of transcripts involved in mitotic cell-cycle progression.
    Fukumura, K, Wakabayashi, S, Kataoka, N, Sakamoto, H, Suzuki, Y, Nakai, K, Mayeda, A, Inoue, K
    Last, Dec. 2016, International Journal of Molecular Sciences, 17(8) (8), 1153, English
    [Refereed]
    Scientific journal

  • Koichi Yamamoto, Mari T. Furukawa, Kazuhiro Fukumura, Arisa Kawamura, Tomoko Yamada, Hitoshi Suzuki, Tetsuro Hirose, Hiroshi Sakamoto, Kunio Inoue
    Pre-mRNA splicing is widely repressed upon heat shock in eukaryotic cells. However, it has been shown that HSP105 pre-mRNA is alternatively spliced in response to heat stress. Using RNAi screening in HeLa cells, we found that RNA-binding proteins hnRNP K and PSF/SFPQ are necessary for the exon 12 exclusion of HSP105 during heat stress. Moreover, exon array analyses showed that a group of genes is alternatively spliced during heat stress in an hnRNP K-dependent manner, whereas hnRNP K is not necessary for the stress-induced alternative splicing of the remaining genes. Among the latter group, we found that SRp38/SRSF10 and SC35/SRSF2 are necessary for the inclusion of exon 13 of TNRC6A during heat stress. Thus, our study clearly showed that several RNA-binding proteins are involved in the splicing regulation in response to heat stress in mammalian cells.
    WILEY-BLACKWELL, Sep. 2016, GENES TO CELLS, 21(9) (9), 1006 - 1014, English
    [Refereed]
    Scientific journal

  • Takashi Miwa, Teruaki Takasaki, Kunio Inoue, Hiroshi Sakamoto
    The chromodomain protein MRG-1 is an essential maternal factor for proper germline development that protects germ cells from cell death in C.elegans. Unlike germ granules, which are exclusively segregated to the germline blastomeres at each cell division from the first cleavage of the embryo, MRG-1 is abundant in all cells in early embryos and is then gradually restricted to the primordial germ cells (PGCs) by the morphogenesis stage. Here, we show that this characteristic spatiotemporal expression pattern is dictated by the mrg-1 3'UTR and is differentially regulated at the RNA level between germline and somatic cells. Asymmetric segregation of germ granules is not necessary to localize MRG-1 to the PGCs. We found that MES-4, an essential chromatin regulator in germ cells, also accumulates in the PGCs in a germ granule-independent manner. We propose that C.elegans PGCs have a novel mechanism to accumulate at least some chromatin-associated proteins that are essential for germline immortality.
    WILEY-BLACKWELL, Nov. 2015, GENES TO CELLS, 20(11) (11), 932 - 942, English
    [Refereed]
    Scientific journal

  • Mari T. Furukawa, Hiroshi Sakamoto, Kunio Inoue
    HERMES, also called RBPMS, is a conserved RNA binding protein with a single RNA recognition motif (RRM) that is abundantly expressed in retinal ganglion cells (RGCs) and in the heart in vertebrates. Here, we identified NonO and PSF as the interacting proteins of HERMES only when the neuronal differentiation of the retinal cell line RGC-5 was induced. Although NonO and PSF are nuclear paraspeckle components, these proteins formed cytoplasmic granules with HERMES in the neurites. G3BP1, a component of stress granules, was also colocalized to the granules, interacting with NonO and HERMES even in the absence of cellular stress. Consistent with a previous report that KIF5 interacts with neuronal granules, the localization of KIF5A overlapped with the cytoplasmic granules in differentiated RGC-5 cells. Thus, our study strongly suggests that the cytoplasmic granule containing HERMES, NonO, PSF, and G3BP1 is a neuronal RNA-protein granule that is transported in neurites during retinal differentiation.
    WILEY-BLACKWELL, Apr. 2015, GENES TO CELLS, 20(4) (4), 257 - 266, English
    [Refereed]
    Scientific journal

  • Shiho Makino, Yuichiro Mishima, Kunio Inoue, Toshifumi Inada
    The CCR4-NOT complex, the major deadenylase in eukaryotes, plays crucial roles in gene expression at the levels of transcription, mRNA decay, and protein degradation. GW182/TNRC6 proteins, which are core components of the microRNAinduced silencing complex in animals, stimulate deadenylation and repress translation via recruitment of the CCR4-NOT complex. Here we report a heterologous experimental system that recapitulates the recruitment of CCR4-NOT complex by TNRC6 in S. cerevisiae. Using this system, we characterize conserved functions of theCCR4-NOTcomplex. The complex stimulates degradation of mRNA from the 5' end by Xrn1, in a manner independent of both translation and deadenylation. This degradation pathway is probably conserved in miRNA-mediated gene silencing in zebrafish. Furthermore, the mRNA fate modulators Dhh1 and Pat1 redundantly stimulatemRNAdecay, but both factors are required for poly(A) tail-independent translation repression by tethered TNRC6A. Our tethering-based reconstitution system reveals that the conserved architecture of Not1/CNOT1 provides a binding surface for TNRC6, thereby connecting microRNA-induced silencing complex to the decapping machinery as well as the translation apparatus.
    AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, Mar. 2015, JOURNAL OF BIOLOGICAL CHEMISTRY, 290(13) (13), 8331 - 8347, English
    [Refereed]
    Scientific journal

  • Taiju Saito, Martin Psenicka, Rie Goto, Shinji Adachi, Kunio Inoue, Katsutoshi Arai, Etsuro Yamaha
    Primordial germ cells (PGCs) arise elsewhere in the embryo and migrate into developing gonadal ridges during embryonic development. In several model animals, formation and migration patterns of PGCs have been studied, and it is known that these patterns vary. Sturgeons (genus Acipenser) have great potential for comparative and evolutionary studies of development. Sturgeons belong to the super class Actinoptergii, and their developmental pattern is similar to that of amphibians, although their phylogenetic position is an out-group to teleost fishes. Here, we reveal an injection technique for sturgeon eggs allowing visualization of germplasm and PGCs. Using this technique, we demonstrate that the PGCs are generated at the vegetal pole of the egg and they migrate on the yolky cell mass toward the gonadal ridge. We also provide evidence showing that PGCs are specified by inheritance of maternally supplied germplasm. Furthermore, we demonstrate that the migratory mechanism is well-conserved between sturgeon and other remotely related teleosts, such as goldfish, by a single PGCs transplantation (SPT) assay. The mode of PGCs specification in sturgeon is similar to that of anurans, but the migration pattern resembles that of teleosts.
    PUBLIC LIBRARY SCIENCE, Feb. 2014, PLOS ONE, 9(2) (2), English
    [Refereed]
    Scientific journal

  • Yumiko Kato, Rie Kusakabe, Kunio Inoue, Shin Tochinai
    MicroRNAs (miRNAs) comprise a group of small noncoding RNA molecules thought to have contributed to the evolution of vertebrate brain homogeneity and diversity. The miRNA miR-124 is well conserved between invertebrates and vertebrates and is expressed abundantly in the central nervous system (CNS). We identified miR-124 in the medaka, Oryzias latipes, and investigated its role in neural development. The five candidate genes for medaka precursor miR-124 are unlinked on four different chromosomes and differ in nucleotide length. Their sequences suggest that they can generate functional miRNAs through conventional miRNA biogenesis by folding into stem-loop structures. Whole-mount in situ hybridization and northern blotting revealed that mature miR-124 is specifically expressed in the CNS and the eyes starting at two days post-fertilization. We also examined the sequences and expression of medaka Polypyrimidine tract binding protein 1 (Ptbp1), a possible direct target of miR-124. The 3'UTR of medaka Ptbp1 contains predicted binding motifs (target sites) for miR-124. A GFP reporter assay for the target sites or the entire 3'UTR showed that exogenous miR-124 silences PTBP1 expression in vivo. Our study suggests that medaka miR-124 is involved in post-transcriptional regulation of target genes in neural development and that medaka miR-124 homologs may have spatiotemporal roles different from those in other vertebrates.
    ZOOLOGICAL SOC JAPAN, Nov. 2013, ZOOLOGICAL SCIENCE, 30(11) (11), 891 - 900, English
    [Refereed]
    Scientific journal

  • Rie Kusakabe, Saori Tani, Koki Nishitsuji, Miyuki Shindo, Kohji Okamura, Yuki Miyamoto, Kenta Nakai, Yutaka Suzuki, Takehiro G. Kusakabe, Kunio Inoue
    Muscle-specific miR-1/206 and miR-133 families have been suggested to play fundamental roles in skeletal and cardiac myogenesis in vertebrates. To gain insights into the relationships between the divergence of these miRs and muscular tissue types, we investigated the expression patterns of miR-1 and miR-133 in two ascidian Ciona species and compared their genomic structures with those of other chordates. We found that Ciona intestinalis and Ciona savignyi each possess a single copy of the miR-1/miR-133 cluster, which is only 350 nucleotide long. During embryogenesis, Ciona miR-1 and miR-133 are generated as a single continuous primary transcript accumulated in the nuclei of the tail muscle cells, starting at the gastrula stage. In adults, mature miR-133 and miR-1 are differentially expressed in the heart and body wall muscle. Expression of the reporter gene linked to the 850-bp upstream region of the predicted transcription start site confirmed that this region drives the muscle-specific expression of the primary transcript of miR-1/miR-133. In many deuterostome lineages, including that of Ciona, the miR-1/133 cluster is located in the same intron of the mind bomb (mib) gene in reverse orientation. Our results suggest that the origin of genomic organization and muscle-specific regulation of miR-1/133 can be traced back to the ancestor of chordates. Duplication of this miR cluster might have led to the remarkable elaboration in the morphology and function of skeletal muscles in the vertebrate lineage. (C) 2012 Elsevier B.V. All rights reserved.
    Last, ELSEVIER SCIENCE BV, Jan. 2013, GENE EXPRESSION PATTERNS, 13(1-2) (1-2), 43 - 50, English
    [Refereed]
    Scientific journal

  • Masami Shiimori, Kunio Inoue, Hiroshi Sakamoto
    The exon junction complex (EJC) is highly conserved in many organisms and is involved in various steps of mRNA metabolism. During the course of investigating the role of EJC in the germ line sex determination of the nematode Caenorhabditis elegans, we found that depletion of one of the three core subunits (Y14, MAG-1, and eukaryotic translation initiation factor 4III [eIF4AIII]) or one auxiliary subunit (UAP56) of EJC resulted in the cytoplasmic leakage of unspliced RNAs from almost all of the C. elegans protein-coding genes examined thus far. This leakage was also observed with the depletion of several splicing factors, including SF3b, IBP160, and PRP19, all of which genetically interacted with Y14. We also found that Y14 physically interacts with both pre-mRNA and spliceosomal U snRNAs, especially U2 snRNA, and that the interaction was abolished when both IBP160 and PRP19 were depleted. Our results strongly suggest that a specific set of EJC subunits is recruited onto introns and interacts with components of the spliceosome, including U2 snRNP, to provide a critical signal for the surveillance and nuclear retention of unspliced RNAs in C. elegans.
    AMER SOC MICROBIOLOGY, Jan. 2013, MOLECULAR AND CELLULAR BIOLOGY, 33(2) (2), 444 - 456, English
    [Refereed]
    Scientific journal

  • Tani, Saori, Kuraku, Shigehiro, Sakamoto, Hiroshi, Inoue, Kunio, Kusakabe, Rie
    2013, Evolution & Development, 15(4) (4), 293 - 304, English
    [Refereed]
    Scientific journal

  • Toshinobu Fujiwara, Akira Fukao, Yumi Sasano, Hidenori Matsuzaki, Ushio Kikkawa, Hiroaki Imataka, Kunio Inoue, Shogo Endo, Nahum Sonenberg, Christian Thoma, Hiroshi Sakamoto
    The RNA binding protein HuD plays essential roles in neuronal development and plasticity. We have previously shown that HuD stimulates translation. Key for this enhancer function is the linker region and the poly(A) binding domain of HuD that are also critical for its function in neurite outgrowth. Here, we further explored the underlying molecular interactions and found that HuD but not the ubiquitously expressed HuR interacts directly with active Akt1. We identify that the linker region of HuD is required for this interaction. We also show by using chimeric mutants of HuD and HuR, which contain the reciprocal linker between RNA-binding domain 2 (RBD2) and RBD3, respectively, and by overexpressing a dominant negative mutant of Akt1 that the HuD-Akt1 interaction is functionally important, as it is required for the induction of neurite outgrowth in PC12 cells. These results suggest the model whereby RNA-bound HuD functions as an adapter to recruit Akt1 to trigger neurite outgrowth. These data might also help to explain how HuD enhances translation of mRNAs that encode proteins involved in neuronal development.
    OXFORD UNIV PRESS, Mar. 2012, NUCLEIC ACIDS RESEARCH, 40(5) (5), 1944 - 1953, English
    [Refereed]
    Scientific journal

  • Yuichiro Mishima, Akira Fukao, Tomoyoshi Kishimoto, Hiroshi Sakamoto, Toshinobu Fujiwara, Kunio Inoue
    MicroRNA (miRNA) is a class of small noncoding RNA approximately 22 nt in length. Animal miRNA silences complementary mRNAs via translational inhibition, deadenylation, and mRNA degradation. However, the underlying molecular mechanisms remain unclear. A key question is whether these three outputs are independently induced by miRNA through distinct mechanisms or sequentially induced within a single molecular pathway. Here, we successfully dissected these intricate outputs of miRNA-mediated repression using zebrafish embryos as a model system. Our results indicate that translational inhibition and deadenylation are independent outputs mediated by distinct domains of TNRC6A, which is an effector protein in the miRNA pathway. Translational inhibition by TNRC6A is divided into two mechanisms: PAM2 motif-mediated interference of poly(A)-binding protein (PABP), and inhibition of 5' cap- and poly(A) tail-independent step(s) by a previously undescribed P-GL motif. Consistent with these observations, we show that, in zebrafish embryos, miRNA inhibits translation of the target mRNA in a deadenylation- and PABP-independent manner at early time points. These results indicate that miRNA exerts multiple posttranscriptional outputs via physically and functionally independent mechanisms and that direct translational inhibition is central to miRNA-mediated repression.
    NATL ACAD SCIENCES, Jan. 2012, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(4) (4), 1104 - 1109, English
    [Refereed]
    Scientific journal

  • Takahiro Nishibu, Yukinobu Hayashida, Saori Tani, Sadamu Kurono, Kanako Kojima-Kita, Ryo Ukekawa, Tsutomu Kurokawa, Satomi Kuramochi-Miyagawa, Toru Nakano, Kunio Inoue, Susumu Honda
    MIWI is one of the PIWI subfamily of proteins mainly expressed in mouse germ cells, and associates with pachytene piRNAs. MIWI has been thought to play an essential role in spermatogenesis and spermiogenesis via biogenesis and/or stability of pachytene piRNAs, retrotransposon silencing, and post-transcriptional regulation of target mRNAs. However, MIWI's detailed role and function are not well understood. In this study, we produced an anti-MIWI mouse monoclonal antibody and identified MIWI-associated poly(A) RNAs by immunoprecipitation from adult mouse testes lysates. Approximately 70% of the MIWI-associated poly(A) RNAs were known mRNAs and 30% of them were unknown non-coding RNAs. These poly(A) RNAs contained piRNA-encoding RNAs transcribed from piRNA cluster regions and piRNA-encoding mRNA, such as Aym1 mRNA. Mature piRNAs specifically encoded in these piRNA-encoding RNAs were generated in pachytene spermatocytes and not detected in Miwi-deficient (Miwi(-/-)) testes. Moreover, MIWI associated with a large number of known mRNAs whose expression levels were increased in pachytene spermatocytes, and the expression of these mRNAs was decreased in Miwi(-/-) testes at 20 days postpartum when pachytene spermatocytes were most abundant. These results strongly suggest that MIWI is involved in pachytene piRNA biogenesis and the positive regulation of target mRNA metabolism in pachytene spermatocytes via association with pachytene piRNA precursors and target mRNAs.
    IRCA-BSSA, 2012, BIOSCIENCE TRENDS, 6(5) (5), 248 - 261, English
    [Refereed]
    Scientific journal

  • Yuki Fujiwara, Katsumi Kasashima, Kuniaki Saito, Miho Fukuda, Akira Fukao, Yumi Sasano, Kunio Inoue, Toshinobu Fujiwara, Hiroshi Sakamoto
    RNA-binding proteins (RBPs) play a vital role in the post-transcriptional regulation of gene expression during neuronal differentiation and synaptic plasticity. One such RBP family, the neuronal Hu protein family, serves as an early marker of neuronal differentiation and targets several mRNAs containing adenine/uridine-rich elements. Recently, we reported that one of the neuronal Hu proteins, HuD stimulates cap-dependent translation through interactions with eIF4A and poly (A) tail. Nevertheless, little is known with respect to how neuronal Hu proteins contribute to the local translation of target mRNAs in neuronal differentiation. Here, we found that neuronal Hu proteins, but not the ubiquitously expressed HuR protein, directly interact with the light chain of microtubule-associated proteins MAP1B (LC1). We also show that HuD simultaneously binds both RNA and LC1 in vitro and that it tightly associates with microtubules in cells in an LC1-dependent manner, raising the possibility that HuD recruits target mRNAs to microtubules. These results uncover the neuronal binding partners for neuron-specific Hu proteins and suggest the involvement of Hu proteins in microtubule-mediated regulation of mRNA expression within neuronal processes. (C) 2011 Elsevier Masson SAS. All rights reserved.
    ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, May 2011, BIOCHIMIE, 93(5) (5), 817 - 822, English
    [Refereed]
    Scientific journal

  • Saori Tani, Rie Kusakabe, Kiyoshi Naruse, Hiroshi Sakamoto, Kunio Inoue
    MicroRNAs (miRNAs, miRs) are short noncoding RNA molecules that negatively control the target mRNAs by binding to the 3' untranslated region (UTR). Previous studies have demonstrated that miR-430 is encoded by a clustered multigene family and is abundantly expressed in early development. In zebrafish, miR-430 is needed to suppress primordial germ cell (PGC)-specific genes, such as nanos1, in somatic cells. However, the molecular characteristics of the miR-430 family in other teleost species have not been reported, and it is unclear whether such a function of miR-430 in PGC specification is a conserved feature of animals or not. In medaka (Oryzias latipes), a distantly related teleost, it has been suggested that PGC might be established in a different mode of specification from that of zebrafish. We characterized 16 miR-430 precursors in the medaka genomic sequence. These miR-430 genes form clusters on chromosome 4, which might share its evolutionary origin with that of the very large miR-430 clusters in zebrafish chromosome 4. However, none of the medaka miR-430 genes are identical to the zebrafish miR-430 paralogs. Medaka miR-430 expression starts during epiboly and decreases after axis formation. Functional analysis using reporter gene constructs showed that miR-430 repressed protein expression by binding to the 3'UTR of zebrafish TDRD7. Consistently, the 3'UTR of medaka TDRD7 contains at least two significant candidates for the putative miR-430 binding site. The ubiquitous and early expression of medaka miR-430 and its ability to downregulate GFP:TDRD7 reporter mRNA imply that miR-430 has a conserved role in early embryogenesis. Smaller copy numbers of miR-430 genes and relatively brief expression in medaka might represent the characteristics of this miRNA family in the common ancestor of teleosts. Changes in the relationships between miR-430 and the target mRNA might be related to differences in the localization patterns of PGC-related genes in medaka and zebrafish. (C) 2009 Elsevier B.V. All rights reserved.
    ELSEVIER SCIENCE BV, Jan. 2010, GENE, 449(1-2) (1-2), 41 - 49, English
    [Refereed]
    Scientific journal

  • [Molecular mechanism of exon definition and alternative splicing].
    Fukumura K, Inoue K
    Dec. 2009, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 54(16 Suppl) (16 Suppl), 2032 - 2037, Japanese, Domestic magazine
    [Refereed]
    Scientific journal

  • Akira Fukao, Yumi Sasano, Hiroaki Imataka, Kunio Inoue, Hiroshi Sakamoto, Nahum Sonenberg, Christian Thoma, Toshinobu Fujiwara
    The RNA-binding protein HuD promotes neuronal differentiation by an unknown mechanism. Here we identify an enhancer function of HuD in translation. Translation stimulation by HuD requires both a 3' poly(A) tail and a 5' m(7)G cap structure. We also show that HuD directly interacts with eIF4A. This interaction and the poly(A)-binding activity of HuD are critical for its translational enhancer function because HuD-eIF4A- and HuD-poly(A)-binding mutants fail to stimulate translation. We show that translation of HCV IRES mRNA, which is eIF4A independent, is not stimulated by HuD. We also find that the eIF4A and poly(A)-binding activities of HuD are not only important for stimulating translation but also are essential for HuD-induced neurite outgrowth in PC12 cells. This example of cap-dependent translational regulation might explain at least in part how HuD triggers the induction of neuronal differentiation.
    CELL PRESS, Dec. 2009, MOLECULAR CELL, 36(6) (6), 1007 - 1017, English
    [Refereed]
    Scientific journal

  • Yasuaki Takeda, Yuichiro Mishima, Toshinobu Fujiwara, Hiroshi Sakamoto, Kunio Inoue
    Background: During zebrafish embryogenesis, microRNA (miRNA) miR-430 contributes to restrict Nanos1 and TDRD7 to primordial germ cells (PGCs) by inducing mRNA deadenylation, mRNA degradation, and translational repression of nanos1 and tdrd7 mRNAs in somatic cells. The nanos1 and tdrd7 3'UTRs include cis-acting elements that allow activity in PGCs even in the presence of miRNA-mediated repression. Methodology/Principal Findings: Using a GFP reporter mRNA that was fused with tdrd7 3'UTR, we show that a germline-specific RNA-binding protein DAZ-like (DAZL) can relieve the miR-430-mediated repression of tdrd7 mRNA by inducing poly(A) tail elongation (polyadenylation) in zebrafish. We also show that DAZL enhances protein synthesis via the 3'UTR of dazl mRNA, another germline mRNA targeted by miR-430. Conclusions/Significance: Our present study indicated that DAZL acts as an "anti-miRNA factor'' during vertebrate germ cell development. Our data also suggested that miRNA-mediated regulation can be modulated on specific target mRNAs through the poly(A) tail control.
    PUBLIC LIBRARY SCIENCE, Oct. 2009, PLOS ONE, 4(10) (10), English
    [Refereed]
    Scientific journal

  • A novel application of metabolomics in vertebrate development
    Shunsuke Hayashi, Shinichi Akiyama, Yutaka Tamaru, Yasuaki Takeda, Toshinobu Fujiwara, Kunio Inoue, Akio Kobayashi, Shingo Maegawa, Eiichiro Fukusaki
    Many Studies have demonstrated the functions of individual genes associated with embryogenesis and have determined the genome sequences of several organisms. Despite the availability of enormous amount of genetic information, dynamic changes that occur during embryogenesis have not yet been completely understood. In order to understand the dynamic processes involved in embryogenesis, we employed the metabolomic approach. The results of our study indicated that there is a close correlation between metabolomes and developmental stages. Our method enables the identification of embryonic stages using metabolomes as "fingerprints." In this manner, we could successfully predict embryonic development on the basis of metabolomic fingerprints. This is the first report describing a model for predicting vertebrate development by using metabolomics. (C) 2009 Elsevier Inc. All rights reserved.
    ACADEMIC PRESS INC ELSEVIER SCIENCE, Aug. 2009, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 386(1) (1), 268 - 272, English
    [Refereed]
    Scientific journal

  • Kazuhiro Fukumura, Ichiro Taniguchi, Hiroshi Sakamoto, Mutsuhito Ohno, Kunio Inoue
    U1 snRNP plays a crucial role in the 5 splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1 (hF1) intron 9 in vitro. Moreover, hF1 intron 9 was efficiently spliced even in U1-disrupted Xenopus oocytes as well as in U1-inactivated HeLa nuclear extracts. Finally, hF1 exon 9 skipping induced by an alternative splicing regulator Fox-1 was impaired when intron 9 was changed to the U1-dependent one. Our results suggest that U1-independent splicing contributes to the regulation of alternative splicing of a class of pre-mRNAs.
    OXFORD UNIV PRESS, Apr. 2009, NUCLEIC ACIDS RESEARCH, 37(6) (6), 1907 - 1914, English
    [Refereed]
    Scientific journal

  • Kusakabe, Rie, Kuraku, Shigehiro, Kuratani, Shigeru, Inoue, Kunio
    2009, Mechanisms of Development, 126
    [Refereed]
    Scientific journal

  • Localization of c- mos mRNA around the animal pole in the zebrafish oocyte with Zor-1/Zorba
    Hitoshi Suzuki, Toshifumi Tsukahara, Kunio Inoue
    In oocytes, many maternally supplied products are stored, and these products play important roles in cell cycle regulation and early development. Mos protein, which is coded on the c-mos gene, promotes oocyte maturation and is involved in MAP-kinase signaling pathway. In Xenopus, maternally supplied c-mos mRNA undergoes poly(A) addition, and translational activation via CPE (cytoplasmic polyadenylation element) and CPEB (CPE-binding protein). The elongated poly(A) is shortened and the c-mos mRNA is degraded during early embryogenesis via EDEN (embryo deadenylation element) and EDEN-BP (EDEN-binding protein). We cloned the full-length zebrafish c-mos gene, which is conserved at the protein coding region in vertebrates. c-mos mRNA has two putative CPE sequences in its 3'UTR, which binds to zebrafish CPEB homologous protein, Zor-1. We could not observe EDEN sequence, and could not detect interaction between c-mos mRNA and zebrafish EDEN-BP homologous protein, Brul, even though immuno precipitation and RT-PCR experiments suggested that c-mos mRNA interacts with Zor-1 in vivo. Interestingly, we found c-mos mRNA is located in the animal cortex of zebrafish oocyte, where Zor-1 protein exists. Taken together, these results suggest that the animal cortex is the central core of oocyte maturation in zebrafish.
    IRCA-BSSA, 2009, BIOSCIENCE TRENDS, 3(3) (3), 96 - 104, English
    [Refereed]
    Scientific journal

  • Yukinobu Hayashida, Takahiro Nishibu, Kunio Inoue, Tsutomu Kurokawa
    Background. Identifying the endogenous RNA induced silencing complex(RISC)-associated RNAs is essential for understanding the cellular regulatory networks by miRNAs. Recently, isolation of RISC-associated mRNAs using antibody was reported, but their method needs a large amount of initial materials. We tried to improve the protocol and constructed an efficient and convenient system for analyzing miRNA and mRNA contents in RISC. Findings. With our protocol, it is possible to clone both miRNAs and mRNAs from the endogenous RISC-associated RNAs immunoprecipitated from less than 107 cells, and we show the ability of our system to isolate the particular target mRNAs for a specific miRNA from the RISC-associated mRNAs using well-characterized miR-122 as an example. After introduction of miR-122 into HepG2 cells, we found several cDNA clones that have miR-122 target sequences. Four of these clones that were concentrated in RISC but decreased in total RNA fraction are expected to be miR-122 target candidates. Interestingly, we found substantial amounts of Alu-related sequences, including both free Alu RNA and Alu-embedded mRNA, which might be one of the general targets for miRNA, in the cDNA clones from the RISC-associated mRNAs. Conclusion. Our method thus enables us to examine not only dynamic changes in miRNA and mRNA contents in RISC but also the relationship of miRNA and target mRNA. We believe that our method can contribute to understanding cellular regulatory networks by miRNAs. © 2009 Hayashida et al licensee BioMed Central Ltd.
    2009, BMC Research Notes, 2, English
    [Refereed]
    Scientific journal

  • Yumi Sasano, Yusuke Hokii, Kunio Inoue, Hiroshi Sakamoto, Chisato Ushida, Toshinobu Fujiwara
    U3 small nucleolar RNA (snoRNA) is one of the members of the box C/D class of snoRNA and is essential for ribosomal RNA (rRNA) processing to generate 18S rRNA in the nucleolus. Although U3 snoRNA is abundant, and is well conserved from yeast to mammals, the genes encoding U3 snoRNA in C. elegans have long remained unidentified. A recent RNomics study in C. elegans predicted five distinct U3 snoRNA genes. However, characterization of these candidates for U3 snoRNA has yet to be performed. In this study, we isolated and characterized four candidate RNAs for U3 snoRNA from the immunoprecipitated RNAs of C. elegans using an antibody against the 2,2,7-trimethylguanosine (TMG) cap. The sequences were identical to the predicted U3 sequences in the RNomics study. Here, we show the several lines of evidence that the isolated RNAs are the true U3 snoRNAs of C. elegans. Moreover, we report the novel expression pattern of U3 snoRNA and fibrillarin, which is an essential component of U3 small nucleolar ribonucleoprotein complex, during early embryo development of C. elegans. To our knowledge, this is the first observation of the inconsistent localization U3 snoRNA and fibrillarin during early embryogenesis, providing novel insight into the mechanisms of nucleologenesis and ribosome production during early embryogenesis. (C) 2008 Published by Elsevier Masson SAS.
    ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, Jun. 2008, BIOCHIMIE, 90(6) (6), 898 - 907, English
    [Refereed]
    Scientific journal

  • Kazuhiro Fukumura, Ayako Kato, Yui Jin, Takashi Ideue, Tetsuro Hirose, Naoyuki Kataoka, Toshinobu Fujiwara, Hiroshi Sakamoto, Kunio Inoue
    Fox-1 is a regulator of tissue-specific splicing, via binding to the element (U) GCAUG in mRNA precursors, in muscles and neuronal cells. Fox-1 can regulate splicing positively or negatively, most likely depending on where it binds relative to the regulated exon. In cases where the (U) GCAUG element lies in an intron upstream of the alternative exon, Fox-1 protein functions as a splicing repressor to induce exon skipping. Here we report the mechanism of exon skipping regulated by Fox-1, using the hF1 gamma gene as a model system. We found that Fox-1 induces exon 9 skipping by repressing splicing of the downstream intron 9 via binding to the GCAUG repressor elements located in the upstream intron 8. In vitro splicing analyses showed that Fox-1 prevents formation of the pre-spliceosomal early (E) complex on intron 9. In addition, we located a region of the Fox-1 protein that is required for inducing exon skipping. Taken together, our data show a novel mechanism of how RNA-binding proteins regulate alternative splicing.
    OXFORD UNIV PRESS, Aug. 2007, NUCLEIC ACIDS RESEARCH, 35(16) (16), 5303 - 5311, English
    [Refereed]
    Scientific journal

  • Kyoko Kosaka, Koichi Kawakami, Hiroshi Sakamoto, Kunio Inoue
    In zebrafish, primordial germ cells (PGCs) are determined by a specialized maternal cytoplasm, the germ plasm, which forms at the distal ends of the cleavage furrows in 4-cell embryos. The germ plasm includes maternal mRNAs from the germline-specific genes such as vasa and nanos1, and vegetally localized dazl RNA is also incorporated into the germ plasm. However, little is known about the distributions and assembly mechanisms of germ plasm components, especially during oogenesis. Here we report that the germ plasm RNAs vasa, nanos1, and dazl co-localize with the mitochondrial cloud (MC) and are transported to the vegetal cortex during early oogenesis. We found that a mitochondrial cloud localization element (MCLE) previously identified in the 3' untranslated region (3'UTR) of Xenopus Xcat2 gene can direct RNA localization to the vegetal cortex via the MC in zebrafish oocytes. In addition, the RNA-binding protein Hermes is a component of the MC in zebrafish oocytes, as is the case in Xenopus. Moreover, we provide evidence that the dazl 3'UTR possesses at least three types of cis-acting elements that direct multiple steps in the localization process: MC localization, anchorage at the vegetal cortex, and localization at the cleavage furrows. Taken together, the data show that the MC functions as a conserved feature that participates in transport of the germ plasm RNAs in Xenopus and zebrafish oocytes. Furthermore, we propose that the germ plasm components are assembled in a stepwise and spatiotemporally-regulated manner during oogenesis and early embryogenesis in zebrafish. (c) 2007 Elsevier Ireland Ltd. All rights reserved.
    ELSEVIER SCIENCE BV, Apr. 2007, MECHANISMS OF DEVELOPMENT, 124(4) (4), 279 - 289, English
    [Refereed]
    Scientific journal

  • Teruaki Takasaki, Zheng Liu, Yasuaki Habara, Kiyoji Nishiwaki, Jun-ichi Nakayama, Kunio Inoue, Hiroshi Sakamoto, Susan Strome
    MRG15, a mammalian protein related to the mortality factor MORF4, is required for cell proliferation and embryo survival. Our genetic analysis has revealed that the Caenorhabditis elegans ortholog MRG-1 serves similar roles. Maternal MRG-1 promotes embryo survival and is required for proliferation and immortality of the primordial germ cells (PGCs). As expected of a chromodomain protein, MRG-1 associates with chromatin. Unexpectedly, it is concentrated on the autosomes and not detectable on the X chromosomes. This association is not dependent on the autosome-enriched protein MES-4. Focusing on possible roles of MRG-1 in regulating gene expression, we determined that MRG-1 is required to maintain repression in the maternal germ line of transgenes on extrachromosomal arrays, and of several X-linked genes previously shown to depend on MES-4 for repression. MRG-1 is not required for PGCs to acquire transcriptional competence or for the turn-on of expression of several PGC-expressed genes (pgl-1, glh-1, glh-4 and nos-1). By contrast to this result in PGCs, MRG-1 is required for ectopic expression of those germline genes in somatic cells lacking the NuRD complex component MEP-1. We discuss how an autosome-enriched protein might repress genes on the X chromosome, promote PGC proliferation and survival, and influence the germ versus soma distinction.
    COMPANY OF BIOLOGISTS LTD, Feb. 2007, DEVELOPMENT, 134(4) (4), 757 - 767, English
    [Refereed]
    Scientific journal

  • Yuichlro Mishima, Antonio J. Giraldez, Yasuaki Takeda, Toshinobu Fujiwara, Hiroshi Sakamoto, Alexander F. Schier, Kunio Inoue
    Early in development, primordial germ cells (PGCs) are set aside from somatic cells and acquire a unique gene-expression program [1].The mechanisms underlying germline-specific gene expression are largely unknown. Nanos expression is required during germline development [2-5] and is posttranscriptionally restricted to PGCs [4,6-8]. Here we report that the microRNA miR-430 targets the 3' untranslated region (UTR) of nanos1 during zebrafish embryogenesis. A miR430 target site within the nanos1 3' UTR reduces poly(A) tail length, mRNA stability, and translation. Repression is disrupted in maternal-zygotic dicer mutants (MZdicer), which lack mature miRNAs [9], and is restored by injection of processed miR-430. Although miR-430 represses other genes equally in germline and soma, specific regions in the nanos1 31 UTR compensate for microRNA-mediated repression in PGCs and allow germline-specific expression. We show that the 3' UTR of an additional PGC-specific gene, TDRD7, is also targeted by miR-430. These results indicate that miR-430 targets the 3' UTRs of germline genes and suggest that differential susceptibility to microRNAs contributes to tissue-specific gene expression.
    CELL PRESS, Nov. 2006, CURRENT BIOLOGY, 16(21) (21), 2135 - 2142, English
    [Refereed]
    Scientific journal

  • AJ Giraldez, Y Mishima, J Rihel, RJ Grocock, S Van Dongen, K Inoue, AJ Enright, AF Schier
    MicroRNAs (miRNAs) comprise 1 to 3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development. By using a microarray approach and in vivo target validation, we find that miR-430 directly regulates several hundred target messenger RNA molecules (mRNAs). Most targets are maternally expressed mRNAs that accumulate in the absence of miR-430. We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis.
    AMER ASSOC ADVANCEMENT SCIENCE, Apr. 2006, SCIENCE, 312(5770) (5770), 75 - 79, English
    [Refereed]
    Scientific journal

  • Taiju Saito, Takafumi Fujimoto, Shingo Maegawa, Kunio Inoue, Minoru Tanaka, Katsutoshi Arai, Etsuro Yamaha
    In some teleost fish, primordial germ cells (PGCs) inherit specific maternal cytoplasmic factors such as vasat and nanos 1 (nos1) mRNA. It has been shown that the 3' untranslated regions (UTRs) of vasa and nos1 have critical roles for stabilization of these RNAs in zebrafish PGCs. In this study, to determine whether this role of the nos1 3'UTR isconserved between teleost species,we injected artificially synthesized mRNA, combining green fluorescent protein (GFP) and the zebrafish nos1 3'UTR (GFP-nos1 3'UTR mRNA), into the fertilized eggs of various fish species. The 3'UTR of the Oryzias latipes vasa homologue (olvas) mRNA was assayed in the same manner. We demonstrate that the PGCs of seven teleost species could be visualized using GFP-nos1 3'UTR mRNA. GFP-olvas 3'UTR mRNA did not identify PGCs in herring or loach embryos, but did enable visualization of the PGCs in medaka embryos. Our results indicate that the 3'UTR of the zebrafish nos1 mRNA can promote maintenance of RNAs in the PGCs of different fish species. Finally, we describe and compare the migration routes of PGCs in seven teleost species.
    U B C PRESS, 2006, INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 50(8) (8), 691 - 700, English
    [Refereed]
    Scientific journal

  • Y Hashimoto, H Suzuki, Y Kageyama, K Yasuda, K Inoue
    Maternally supplied germ plasm is essential for germ lineage establishment in many species, but the molecular details are still largely unknown, especially in vertebrates, and identification of novel factors that localize to germ plasm is desirable. We previously reported that one of the components of zebrafish germ plasm is mRNA of the bruno-like (brul) gene, a homologue of bruno, which, in Drosophila, is known to participate in germ lineage establishment. Here, we show that not only mRNA but also protein of brul is localized to the zebrafish germ plasm at the ends of the cleavage furrows. In 4- and 8-cell stage embryos, Brul protein is localized to the periphery of the blastomeres, as well as to the ends of the cleavage furrows, forming numerous minute particles. These particles appear at the cortex of the fertilized egg within 10 min after fertilization. Surprisingly, these distinctive localizations, as well as the minute particles, completely disappeared by the 16-cell stage, although relatively weak expression was detected ubiquitously throughout embryogenesis. This is the first report of a protein that localizes to the germ plasm in zebrafish. (C) 2005 Elsevier B.V. All rights reserved.
    ELSEVIER SCIENCE BV, Jan. 2006, GENE EXPRESSION PATTERNS, 6(2) (2), 201 - 205, English
    Scientific journal

  • Y Masuhiro, Y Mezaki, M Sakari, KI Takeyama, T Yoshida, K Inoue, J Yanagisawa, S Hanazawa, BW O'Malley, S Kato
    Mitogen-activated protein kinase-mediated growth factor signals are known to augment the ligand-induced transactivation function of nuclear estrogen receptor alpha (ER alpha) through phosphorylation of Ser-118 within the ERa N-terminal transactivation (activation function-1) domain. We identified the spliceosome component splicing factor (SF)3a p120 as a coactivator specific for human ER alpha (hER alpha) activation function-1 that physically associated with ERa dependent on the phosphorylation state of Ser-118. SF3a p120 potentiated hER alpha-mediated RNA splicing, and notably, the potentiation of RNA splicing by S173a p120 depended on hER Ser-118 phosphorylation. Thus, our findings suggest a mechanism by which growth factor signaling can regulate gene expression through the modulation of RNA splicing efficiency via phosphorylation of sequence-specific activators, after association between such activators and the spliceosome.
    NATL ACAD SCIENCES, Jun. 2005, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 102(23) (23), 8126 - 8131, English
    [Refereed]
    Scientific journal

  • NAGAI TERUMI, OTANI SATOSHI, SAITO TAIJU, MAEGAWA SHINGO, INOUE KUNIO, ARAI KATSUTOSHI, YAMAHA ETSURO, TERUMI NAGAKI, SATOSHI OTANI, TAIJU SAITO, SHINGO MAEGAWA, KUNIO INOUE, KATSUTOSHI ARAI, ETSURO YAMAHA, Laboratory of Breeding Science Graduate School of Fisheries Science Hokkaido University:(Present address)Laboratory of Regulatory Biology Department of Biology Faculty of Science Toyama University, Laboratory of Breeding Science Graduate School of Fisheries Science Hokkaido University, Laboratory of Breeding Science Graduate School of Fisheries Science Hokkaido University, Laboratory of Breeding Science Graduate School of Fisheries Science Hokkaido University:(Present address)Department of Biology University of Pennsylvania, Laboratory of Molecular and Developmental Biology Graduate School of Biological Sciences Nara Institute of Science and Technology:(Present address)Graduate School of Science and Technology Kobe University, Laboratory of Breeding Science Graduate School of Fisheries Science Hokkaido University, Nanae Fresh Water Laboratory Field Science Center for Northern Biosphere Hokkaido University
      To elucidate the effects of blastoderm graft for chimeric-fish production on further development, transplantations of upper, lower or entire part of blastoderm were performed onto the animal part of host embryos during the blastula stage in zebrafish. In the case of upper, all resultant chimeric embryos developed normally, while in the lower part, many acephalic embryos appeared. When the entire blastoderm was transplanted, many resultant embryos showed normal phenotype with extra cell-mass, while a few had double axis. vas mRNA, one of the markers of PGCs, also kept the expressions in both donor and recipient blastomeres in transplantation of the entire blastoderm. Donor PGCs were frequently detected at germinal anlagen in histological sections of 6-day larvae developed from transplantations of lower and entire blastoderm, but seldom in those of the upper part. These results might suggest that graft transplantation was effective for production of germ-line chimera, but sometimes caused un-regulaory differentiation of graft cells under the community effect.
    The Japanese Society of Fisheries Science, Jan. 2005, NIPPON SUISAN GAKKAISHI, 71(1) (1), 1 - 9, Japanese
    Scientific journal

  • T Nagai, S Otani, T Saito, S Maegawa, K Inoue, K Arai, E Yamaha
    To elucidate the effects of blastoderm graft for chimeric-fish production on further development, transplantations of upper, lower or entire part of blastoderm were performed onto the animal part of host embryos during the blastula stage in zebrafish. In the case of upper, all resultant chimeric embryos developed normally, while in the lower part, many acephalic embryos appeared. When the entire blastoderm was transplanted, many resultant embryos showed normal phenotype with extra cell-mass, while a few had double axis. vas mRNA, one of the markers of PGCs, also kept the expressions in both donor and recipient blastomeres in transplantation of the entire blastoderm. Donor PGCs were frequently detected at germinal anlagen in histological sections of 6-day larvae developed from transplantations of lower and entire blastoderm, but seldom in those of the upper part. These results might suggest that graft transplantation was effective for production of germ-line chimera, but sometimes caused un-regulaory differentiation of graft cells under the community effect.
    JAPANESE SOC FISHERIES SCIENCE, Jan. 2005, NIPPON SUISAN GAKKAISHI, 71(1) (1), 1 - 9, Japanese
    [Refereed]
    Scientific journal

  • S Otani, T Kitauchi, T Saito, S Sakao, S Maegawa, K Inoue, K Arai, E Yamaha
    The property of primordial germ cells (PGCs) in fragmented goldfish embryos was investigated. When 1- and 2- cell embryos were cut at several perpendicular levels at the animal-vegetal axis, cells expressing vas mRNA were observed in the resultant embryos derived from all kinds of animal fragments. Blastodisc fragments from the 1- to 2-cell stage developed to spherical embryos containing yolk body with a yolk syncytial layer (YSL). Germ ring and no tail expression were not observed in the spherical embryo. When the spherical embryo labeled with tracer dye or GFP-nosl 3'UTR mRNA was transplanted onto the animal part of the blastoderm in a host embryo at the blastula stage, PGCs of spherical embryo origin were detected around the gonadal ridges in the resultant embryos which developed normally. These results suggest that small animal fragments should contain factors sufficient for PGC differentiation and that PGCs differentiate without mesoderm induction, since mesoderm is not induced in a spherical embryo.
    U B C PRESS, 2005, INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 49(7) (7), 843 - 850, English
    Scientific journal

  • K Saito, T Fujiwara, J Katahira, K Inoue, H Sakamoto
    Hu proteins are RNA-binding proteins that are implicated in the control of stabilization, nuclear export, and/or translation of specific mRNAs with AU-rich elements (AREs) in the 3'-untranslated region. Three neuron-specific Hu proteins (HuD, HuB, and HuC), but not a ubiquitously expressed Hu protein HuR, have an activity to induce neurite outgrowth when they are overexpressed in a rat neuronal cell line PC12. Here we show that TAP/NXF1, the primary export receptor for the bulk mRNA, is a specific binding partner for HuD. In vitro binding experiments using recombinant proteins revealed that the interaction between TAP and HuD is direct and that HuD can form a ternary complex together with both TAP and RNA. Interestingly, HuR does not interact with TAP. These results suggest that HuD acts as a novel adaptor protein to recruit TAP for efficient export of ARE-containing mRNAs in neuronal cells. (C) 2004 Elsevier Inc. All rights reserved.
    ACADEMIC PRESS INC ELSEVIER SCIENCE, Aug. 2004, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 321(2) (2), 291 - 297, English
    [Refereed]
    Scientific journal

  • Y Hashimoto, S Maegawa, T Nagai, E Yamaha, H Suzuki, K Yasuda, K Inoue
    Maternally supplied factors in fertilized eggs play essential roles in the establishment of primordial germ cells. In zebrafish, cytoplasm at the distal ends of the first and second cleavage furrows has been assumed to contain germ lineage determinants, since maternal transcripts of genri lineage-specific genes are localized to ends of the cleavage furrows. To investigate whether these parts of cytoplasm are required for germ cell formation, we removed all four regions of the cytoplasm by glass capillary at the 4-cell stage. Histological analysis revealed that the ablation of cytoplasm at the ends of the cleavage planes resulted in a severe reduction in the number of germ cells. In addition, the expression of germ lineage markers was eliminated by cytoplasmic ablation. These results demonstrated that cytoplasm at the distal ends of cleavage furrows is essential for germ cell formation. We also found novel localization patterns for zDazl and brul mRNAs along the cleavage planes. Our findings provide the first direct evidence that localized cytoplasmic factors are indispensable for germ cell establishment in zebrafish. (C) 2004 Elsevier Inc. All rights reserved.
    Last, ACADEMIC PRESS INC ELSEVIER SCIENCE, Apr. 2004, DEVELOPMENTAL BIOLOGY, 268(1) (1), 152 - 161, English
    [Refereed]
    Scientific journal

  • E Saijou, T Fujiwara, T Suzaki, K Inoue, H Sakamoto
    RBD-1 is the Caenorhabditis elegans homolog of Mrd1p, which was recently shown to be required for 18S ribosomal RNA (rRNA) processing in yeast. To gain insights into the relationship between ribosome biogenesis and the development of multicellular organisms, we examined the expression and function of RBD-1. Maternal RBD-1 in the fertilized egg disappears immediately after cleavage starts, whereas zygotic RBD-1 first appears in late embryos and is localized in the nucleolus in most cells, although zygotic transcription of pre-rRNA is known to be initiated as early as the one-cell stage. RNA interference of the rbd-1gene severely inhibits the processing of 18S rRNA in association with various developmental abnormalities, indicating its essential role in pre-rRNA processing and development in C. elegans. These results provide evidence for the linkage between ribosome biogenesis and the control of development and imply unexpected uncoupling of transcription and processing of pre-rRNA in early C. elegans embryos.
    OXFORD UNIV PRESS, Feb. 2004, NUCLEIC ACIDS RESEARCH, 32(3) (3), 1028 - 1036, English
    [Refereed]
    Scientific journal

  • A vertebrate RNA-binding protein Fox-1 regulates tissue-specific splicing via the pentanucleotide GCAUG
    Y Jin, H Suzuki, S Maegawa, H Endo, S Sugano, K Hashimoto, K Yasuda, K Inoue
    Alternative splicing is one of the central mechanisms that regulate eukaryotic gene expression. Here we report a tissue-specific RNA-binding protein, Fox-1, which regulates alternative splicing in vertebrates. Fox-1 bound specifically to a pentanucleotide GCAUG in vitro. In zebrafish and mouse,fox-1 is expressed in heart and skeletal muscles. As candidates for muscle-specific targets of Fox-1, we considered two genes, the human mitochondrial ATP synthase gamma-subunit gene (Fly) and the rat alpha-actinin gene, because their primary transcripts contain several copies of GCAUG. In transfection experiments, Fox-1 induced muscle-specific exon skipping of the F1gamma gene via binding to GCAUG sequences upstream of the regulated exon. Fox-1 also regulated mutually exclusive splicing of the alpha-actinin gene, antagonizing the repressive effect of polypyrimidine tract-binding protein (PTB). It has been reported that GCAUG is essential for the alternative splicing regulation of several genes including fibronectin. We found that Fox-1 promoted inclusion of the fibronectin EIIIB exon. Thus, we conclude that Fox-1 plays key roles in both positive and negative regulation of tissue-specific splicing via GCAUG.
    NATURE PUBLISHING GROUP, Feb. 2003, EMBO JOURNAL, 22(4) (4), 905 - 912, English
    [Refereed]
    Scientific journal

  • S Maegawa, M Yamashita, K Yasuda, K Inoue
    Background: In many species, DAZ homologous genes encode RNA-binding proteins containing two conserved motifs, namely the RNA-recognition motif (RRM) and the DAZ motif. Genetic analysis and gene disruption studies have demonstrated that DAZ family proteins play important roles in gametogenesis. However, little is known about the biochemical functions of DAZ family proteins. Results: Using in vitro selection and UV-crosslinking experiments, we identified the sequence 'GUUC' as the target RNA sequence of zebrafish DAZ-like protein (zDAZL). In transfection experiments, zDAZL protein activated translation in a manner dependent on the binding sequence in the 3'UTR of the Drosophila twine gene or zDazl gene. Moreover, it is highly likely that the zDAZL protein associates with polysomes through the DAZ motif in vivo , and that the association with polysomes is indispensable for translational activation. Conclusions: This is the first report that the DAZ family protein directly promotes the translation of the target mRNAs in vertebrates. This study provides important insights into the molecular mechanisms underlying the post-transcriptional regulation of DAZ family proteins in gametogenesis.
    BLACKWELL PUBLISHING LTD, Sep. 2002, GENES TO CELLS, 7(9) (9), 971 - 984, English
    [Refereed]
    Scientific journal

  • S Otani, S Maegawa, K Inoue, K Arai, E Yamaha
    vas RNA has been identified in germ-line cells and its precursors in zebrafish, with the result that the germ-line lineage can be traced throughout embryogenesis. In the present study, we described vas localization and the migration of vas-positive cells in goldfish, using whole mount in situ hybridization. The signals of vas mRNA localization appeared at the marginal part of the first to third cleavage planes. The eight signals were detected during the period from the 8- cells to the 512-cell stage. At the late-blastula stage, additional numbers of vas-positive cells were observed, suggesting the proliferation of these cells. At the segmentation period, vas-positive cells showed a long extended distribution along the embryonic axis, but did not form any clusters. vas-positive cells were occasionally distributed at the head region, especially around the future otic vesicle. These signals were inherited to the primordial germ cells, suggesting that vas-positive cells were primordial germ cells (PGCs) in goldfish.
    ZOOLOGICAL SOC JAPAN, May 2002, ZOOLOGICAL SCIENCE, 19(5) (5), 519 - 526, English
    [Refereed]
    Scientific journal

  • Regulation of alternative splicing of alpha-actinin transcript by Bruno-like proteins
    H Suzuki, Y Jin, H Otani, K Yasuda, K Inoue
    Background: The Bruno-like or CELF proteins, such as mammalian CUGBP1 and Etr-3, Xenopus EDEN-BP, and Drosophila Bruno (Bru), are regulators of gene expression at the post-transcriptional level, and contain three RNA-recognition motifs (RPMs). It has been shown that mammalian CUGBP1 and Etr-3 regulate alternative splicing of cardiac troponin T pre-mRNA via binding to CUG-triplet repeats. Results: Using in vitro selection and UV-crosslinking experiments, we found that zebrafish Bruno-like proteins bound to repeat elements of uridine and purine (termed UREs). It is known that non-muscle (NM) and smooth muscle (SM) exons of the rat alpha-actinin gene are used in a mutually exclusive manner. Transfection experiments in mammalian cells showed that zebrafish Brul and Etr-3 induced the muscle-specific splicing of rat alpha-actinin pre-mRNA via binding to the URE at the branch point upstream of the NM exon. In contrast, zebrafish Etr-1 promoted skipping of both the NM and SM exons in a manner which was not dependent on URE-binding. Conclusions: Our results showed that Bruno-like proteins bind to UREs and regulate the alternative splicing of alpha-actinin pre-mRNA. Members of the Bruno family play multiple roles in splicing regulation.
    WILEY-BLACKWELL, Feb. 2002, GENES TO CELLS, 7(2) (2), 133 - 141, English
    [Refereed]
    Scientific journal

  • Biochemical identification of Xenopus pumilio as a sequence-specific cyclin B1 mRNA-binding protein that physically interacts with a nanos homolog, Xcat-2, and a cytoplasmic polyadenylation element-binding protein
    S Nakahata, Y Katsu, K Mita, K Inoue, Y Nagahama, M Yamashita
    Translational activation of dormant cyclin B1 mRNA stored in oocytes is a prerequisite for the initiation or promotion of oocyte maturation in many vertebrates. Using a monoclonal antibody against the domain highly homologous to that of Drosophila Pumilio, we have shown for the first time in any vertebrate that a homolog of Pumilio is expressed in Xenopus oocytes, This 137-kDa protein binds to the region including the sequence UGUA at nucleotides 1335-1338 in the 3'-untranslated region of cyclin B1 mRNA, which is close to but does not overlap the cytoplasmic polyadenylation elements (CPEs). Physical in vitro association of Xenopus Pumilio with a Xenopus homolog of Nanos (Xcat-2) was demonstrated by a protein pull-down assay. The results of immunoprecipitation experiments showed in vivo interaction between Xenopus Pumilio and CPE-binding protein (CPEB), a key regulator of translational repression and activation of mRNAs stared in oocytes, This evidence provides a new insight into the mechanism of translational regulation through the 3'-end of mRNA during oocyte maturation. These results also suggest the generality of the function of Pumilio as a translational regulator of dormant mRNAs in both invertebrates and vertebrates.
    AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, Jun. 2001, JOURNAL OF BIOLOGICAL CHEMISTRY, 276(24) (24), 20945 - 20953, English
    [Refereed]
    Scientific journal

  • Hitoshi Suzuki, Shingo Maegawa, Takahiro Nishibu, Tomoyasu Sugiyama, Kunio Yasuda, Kunio Inoue
    Asymmetric distribution of maternal mRNAs has not been well documented in zebrafish. Recently, we have shown that dazl mRNA is localized at the vegetal pole. Here we report a novel zebrafish gene, bruno-like (brul), which provides another example of vegetal mRNA localization. brul encodes an Elav-type RNA-binding protein that belongs to the Bruno-like family that includes mammalian CUG-BP, Xenopus EDEN-BP, and Drosophila Bruno. At 24 hpf, brul mRNA was abundant in lens fiber cells. At the onset of embryogenesis, maternal brul mRNA was detected at the vegetal pole, and it then migrated rapidly toward the blastoderm through yolk cytoplasmic streams. During oogenesis, brul mRNA became localized at the vegetal cortex at stage II, later than dazl mRNA. We found that anchoring of brul mRNA was dependent on microfilaments. © 2000 Elsevier Science Ireland Ltd.
    May 2000, Mechanisms of Development, 93(1-2) (1-2), 205 - 209, English
    [Refereed]
    Scientific journal

  • Maternal mRNA localization of zebrafish DAZ-like gene
    S Maegawa, K Yasuda, K Inoue
    Members of the DAZ gene family encode RNA-binding proteins and have been shown to play a pivotal role in gametogenesis. In Xenopus, a DAZ-like gene encodes an RNA component of the germ plasm. We have identified a zebrafish DAZ homologue, zDazl. zDazl mRNA was expressed in gonads of both sexes. In ovary, it was localized in the cortex of oocytes. At the onset of embryogenesis, maternal zDazl mRNA was detected at the vegetal pole. It migrated toward blastomeres through cytoplasmic streams as early embryogenesis proceeded. This is the first report showing maternal mRNA localization at the vegetal pole in fish and the existence of mRNA streams in the yolk cytoplasm. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
    ELSEVIER SCIENCE BV, Mar. 1999, MECHANISMS OF DEVELOPMENT, 81(1-2) (1-2), 223 - 226, English
    [Refereed]
    Scientific journal

  • Disruption of the splicing enhancer sequence within exon 27 of the dystrophin gene by a nonsense mutation induces partial skipping of the exon and is responsible for Becker muscular dystrophy
    N Shiga, Y Takeshima, H Sakamoto, K Inoue, Y Yokota, M Yokoyama, M Matsuo
    The mechanism of exon skipping induced by nonsense mutations has not been well elucidated, We now report results of in vitro splicing studies which disclosed that a particular example of exon skipping is due to disruption of a splicing enhancer sequence located within the exon, A nonsense mutation (E1211X) due to a G to T transversion at the 28th nucleotide of exon 27 (G3839T) was identified in the dystrophin gene of a Japanese Pecker muscular dystrophy case, Partial skipping of the exon resulted in the production of truncated dystrophin mRNA, although the consensus sequences for splicing at both ends of exon 27 were unaltered, To determine how E1211X induced exon 27 skipping, the splicing enhancer activity of purine-rich region within exon 27 was examined in an in vitro splicing system using chimeric doublesex gene pre-mRNA. The mutant sequence containing G3839T abolished splicing enhancer activity of the wild-type purine-rich sequence for the upstream intron in this chimeric pre-mRNA. An artificial polypurine oligonucleotide mimicking the purine-rich sequence of exon 27 also showed enhancer activity that was suppressed by the introduction of a T nucleotide. Furthermore, the splicing enhancer activity was more markedly inhibited when a nonsense codon was created by the inserted T residue, This is the first evidence that partial skipping of an exon harboring a nonsense mutation is due to disruption of a splicing enhancer sequence.
    ROCKEFELLER UNIV PRESS, Nov. 1997, JOURNAL OF CLINICAL INVESTIGATION, 100(9) (9), 2204 - 2210, English
    [Refereed]
    Scientific journal

  • Interaction of goosecoid and brachyury in Xenopus mesoderm patterning
    M Artinger, Blitz, I, K Inoue, U Tran, KWY Cho
    Detailed in situ analyses reveal overlapping expression of gsc and Xbra in the early Spemann's organizer. Coexpression is lost during gastrulation suggesting an interaction between these genes. Ectopic expression of gsc ventrally suppresses endogenous Xbra expression and transcription from Xbra promoter reporter gene constructs. Suppression is mediated, at least partially, by a gsc-binding site within the first 349 bp of the promoter. Xbra reporter gene transcription is also suppressed in the region of endogenous gsc expression, whereas high-level ectopic Xbra expression has no effect on endogenous gsc expression. We suggest that early patterning of the vertebrate mesoderm, like early patterning of the Drosophila embryo, occurs by first establishing broad domains of gene expression which are subsequently refined by intergenic interactions to further delimit tissue boundaries. (C) 1997 Elsevier Science Ireland Ltd.
    ELSEVIER SCIENCE BV, Jul. 1997, MECHANISMS OF DEVELOPMENT, 65(1-2) (1-2), 187 - 196, English
    [Refereed]
    Scientific journal

  • Takashi Takabatake, Tadashi C. Takahashi, Kunio Inoue, Masanori Ogawa, Kazuhito Takeshima
    We have isolated cDNAs of sonic hedgehog (shh) and fork head from Cynops (Japanese newt) embryo. Their expression was investigated in relation to mesoderm induction by activin and basic fibroblast growth factor (bFGF). Different from these homologs in Xenopus, they are activated not only by activin but also by bFGF in animal cap expiants, showing a difference of animal pole cells in responsiveness to bFGF between Cynops and Xenopus. We also investigated the involvement of fork head in shh activation. The expression of shh was activated in animal caps which overexpressed either of two Xenopus fork head homologs, pintallavis/XFD-1 or XFKH-1/XFD-1′, indicating that fork head up-regulates the transcription of shh in Cynops embryo. O 1996 Academic Press, Inc.
    Academic Press Inc., Jan. 1996, Biochemical and Biophysical Research Communications, 218(1) (1), 395 - 401, English
    [Refereed]
    Scientific journal

  • Molecular mechanisms of Spemann's organizer formation: Conserved growth factor synergy between Xenopus and mouse
    T Watabe, S Kim, A Candia, U Rothbacher, C Hashimoto, K Inoue, KWY Cho
    Mesoderm induction assays in Xenopus have implicated growth factors such as activin, Vg1, Xwnt -8, and noggin as important in directing the formation of dorsal mesoderm (Spemann's organizer). Because these growth factors are structurally very different, they presumably act through distinct cell surface receptors that initiate different intracellular signaling cascades. A consequence of all of these signaling pathways, however, seems to be the induction of goosecoid (gsc) gene expression. To understand how integration of these different signaling pathways results in formation of Spemann's organizer, we sought to identify growth factor-responsive elements within the gsc promoter. Through microinjection of reporter genes we have identified two cis-acting elements, a distal element (DE) and a proximal element (PE), that are required for activin/BVg1 and Wnt induction, respectively. We have shown that the DE mediates activin induction in the absence of protein synthesis and therefore constitutes the first activin response element identified to interpret transforming growth factor-beta (TGF-beta) superfamily member signaling directly. Using a reporter gene construct containing a multimerized DE, we find that an activin/BVg1-type signaling cascade is active throughout the vegetal hemisphere and marginal zone but not in the animal hemisphere. We demonstrate further that both the distal and proximal elements are essential for high-level transcription of the gsc gene, specifically in dorsal mesoderm, strongly suggesting that establishment of Spemann's organizer requires synergistic input from activin/BVg1-like and Wnt signaling pathways. Finally, mechanisms of establishing the organizer are likely to be conserved throughout vertebrate evolution.
    COLD SPRING HARBOR LAB PRESS, Dec. 1995, GENES & DEVELOPMENT, 9(24) (24), 3038 - 3050, English
    [Refereed]
    Scientific journal

  • K HOSHIJIMA, A KOHYAMA, WATAKABE, I, K INOUE, H SAKAMOTO, Y SHIMURA
    Somatic sex determination in Drosophila depends on the expression of Sex-lethal (Sri), whose level is determined by the relative number of X chromosomes and sets of autosomes (X:A ratio), The first step in regulation of Sri expression is transcriptional control from its early promoter and several genes encoding transcription factors of the helix-loop-helix (HLH) family such as daughterless (de), sisterless-b (sis-b) deadpan (dpn) and extramacrochaetae (emc) have been implicated, By the use of transfection assays and in vitro binding experiments, here we show that da/sis-b heterodimers bind several sites on the Sri early promoter with different affinities and consequently tune the level of active transcription from this promoter. Interestingly, our data indicate that repression by the dpn product of da/sis-b dependent activation results from specific binding of dpn protein to a unique site within the promoter, This contrasts with the mode of emc repression, which inhibits the formation of the da/sis-b heterodimers. These results reveal the molecular mechanisms by which Sri gene transcription is positively or negatively regulated to control somatic sex determination.
    OXFORD UNIV PRESS UNITED KINGDOM, Sep. 1995, NUCLEIC ACIDS RESEARCH, 23(17) (17), 3441 - 3448, English
    [Refereed]
    Scientific journal

  • Mechanism of alternative RNA splicing
    K Inoue, Y Shimura
    ELSEVIER SCIENCE PUBL B V, 1995, TRACING BIOLOGICAL EVOLUTION IN PROTEIN AND GENE STRUCTURES, 87 - 95, English
    [Refereed]
    International conference proceedings

  • Aspects of splice site selection in constitutive and alternative pre-mRNA splicing.
    Inoue, K., Ohno, M., Shimura, Y.
    Lead, 1995, Gene Expr., 4(3) (3), 177 - 182
    [Refereed]

  • Control of Drosophila Sex-lethal pre-mRNA splicing by its own female-specific product.
    H Sakamoto, K Inoue, I Higuchi, Y Ono, Y Shimura
    Drosophila melanogaster somatic sexual differentiation is accomplished by serial function of the products of sex-determination genes. Sex-lethal (Sxl), is one such gene. It is functionally expressed only in female flies. The sex-specific expression of this gene is regulated by alternative mRNA splicing which results in either the inclusion or exclusion of the translation stop codon containing third exon. Although previous genetic and molecular analyses suggest that functional Sxl expression is maintained by a positive feedback loop, where the female-specific Sxl product promotes the synthesis of its own female-specific mRNA, the mechanistic details of such regulation have remained unclear. We have developed a cotransfection system using Drosophila cultured (Kc) cells in which Sxl primary transcripts are expressed with or without the female specific Sxl product. Here we show that the female-specific Sxl product induces the synthesis of its own female-specific mRNA by negative control of male-specific splicing. Deletion, substitution, and binding experiments have demonstrated that multiple uridine-rich sequences in the introns around the male-specific third exon are involved in the splicing regulation of Sxl pre-mRNA.
    Nov. 1992, Nucleic acids research, 20(21) (21), 5533 - 40, English, International magazine
    [Refereed]
    Scientific journal

  • Binding of the Drosophila transformer and transformer-2 proteins to the regulatory elements of doublesex primary transcript for sex-specific RNA processing.
    K Inoue, K Hoshijima, I Higuchi, H Sakamoto, Y Shimura
    Sex-specific alternative processing of double-sex (dsx) precursor messenger RNA (pre-mRNA) is one of the key steps that regulates somatic sexual differentiation in Drosophila melanogaster. By transfection analyses using dsx minigene constructs, we identified six copies of the 13-nucleotide sequences TC(T/A)(T/A)C(A/G)ATCAACA in the female-specific fourth exon that act as the cis elements for the female-specific splicing of dsx pre-mRNA. UV-crosslinking experiments revealed that both female-specific transformer (tra) and transformer-2 (tra-2) products bind to the 13-nucleotide sequences of dsx pre-mRNA. These results strongly suggest that the female-specific splicing of dsx pre-mRNA is activated by binding of these proteins to the 13-nucleotide sequences.
    Lead, Sep. 1992, Proceedings of the National Academy of Sciences of the United States of America, 89(17) (17), 8092 - 6, English, International magazine
    [Refereed]

  • CONTROL OF DOUBLESEX ALTERNATIVE SPLICING BY TRANSFORMER AND TRANSFORMER-2 IN DROSOPHILA
    K HOSHIJIMA, K INOUE, HIGUCHI, I, H SAKAMOTO, Y SHIMURA
    Sex-specific alternative processing of doublesex (dsx) precursor messenger RNA (pre-mRNA) regulates somatic sexual differentiation in Drosophila melanogaster. Cotransfection analyses in which the dsx gene and the female-specific transformer (tra) and transformer-2 (tra-2) complementary DNAs were expressed in Drosophila Kc cells revealed that female-specific splicing of the dsx transcript was positively regulated by the products of the tra and tra-2 genes. Furthermore, analyses of mutant constructs of dsx showed that a portion of the female-specific exon sequence was required for regulation of dsx pre-messenger RNA splicing.
    AMER ASSOC ADVANCEMENT SCIENCE, May 1991, SCIENCE, 252(5007) (5007), 833 - 836, English
    [Refereed]
    Scientific journal

  • Binding of the Drosophila sex-lethal gene product to the alternative splice site of transformer primary transcript.
    K Inoue, K Hoshijima, H Sakamoto, Y Shimura
    Somatic sexual differentiation in Drosophila melanogaster is accomplished by a hierarchy of genes of which one, Sex-lethal (Sxl), is required for the functional female-specific splicing of the transcripts of the immediately downstream regulatory gene, transformer (tra). The first exon of the tra primary transcript is spliced to one of two acceptor sites. Splicing to the upstream site yields a messenger RNA which is neither sex-specific nor functional, but that produced after splicing to the downstream acceptor site yields a functional female-specific mRNA. Here we address the question of how the Sxl gene product determines the alternative splicing of tra primary transcripts. One suggestion is that non-sex-specific splicing to the upstream acceptor is blocked in female flies by sex-specific factors, but neither the identity of the female-specific factors nor the mechanism of the blockage has been specified. We have now performed co-transfection experiments in which Sxl complementary DNA and the tra gene are expressed in Drosophila Kc cells. Moreover, we find that female Sxl-encoded protein binds specifically to the tra transcript at or near the non-sex-specific acceptor site, implying that the female Sxl gene product is the trans-acting factor that regulates the alternative splicing.
    Lead, Mar. 1990, Nature, 344(6265) (6265), 461 - 3, English, International magazine
    [Refereed]
    Scientific journal

  • A WATAKABE, K INOUE, H SAKAMOTO, Y SHIMURA
    OXFORD UNIV PRESS UNITED KINGDOM, Oct. 1989, NUCLEIC ACIDS RESEARCH, 17(20) (20), 8159 - 8169, English
    [Refereed]
    Scientific journal

  • Effect of the cap structure on pre-mRNA splicing in Xenopus oocyte nuclei.
    K Inoue, M Ohno, H Sakamoto, Y Shimura
    The effect of the 5' cap structure on the splicing of precursor mRNAs was investigated after the RNAs were injected into Xenopus oocyte nuclei. The precursor mRNAs synthesized in vitro in a prokaryotic transcription system with a dinucleotide, ApppG, as a primer, were extremely stable when injected into the nuclei yet behaved like uncapped pre-mRNAs in the in vitro splicing reaction. The ApppG-primed precursor mRNAs served as a control (uncapped) in the injection experiments, and their splicing reactions were compared with those of their capped (m7GpppG-primed) counterparts. The capped precursors were spliced more efficiently than the uncapped precursors. Examination of splicing of the precursor mRNA that contained three exons and two introns with a single molecule has revealed that the cap structure exerts its effect primarily on the 5'-proximal intron. Thus, the cap structure not only stabilizes precursor mRNAs but also plays a positive role in the splicing of precursor mRNAs in cells.
    Lead, Sep. 1989, Genes & development, 3(9) (9), 1472 - 9, English, International magazine
    [Refereed]
    Scientific journal

■ MISC
  • A Distinct Subset of Human Short Introns: U2AF Heterodimer Is Replaced by SPF45 (RBM17) as General Splicing Factor
    Fukumura K, Yoshimoto R, Hirose T, Inoue K, Mayeda A
    cold spring harbor laboratory, Sep. 2019, bioRxiv, 784868, English, International magazine
    Report scientific journal

  • Rie Kusakabe, Kunio Inoue
    MicroRNAs (miRs) are a group of small RNAs that play a major role in post-transcriptional regulation of gene expression. In animals, many of the miRs are expressed in a conserved spatiotemporal manner. Muscle tissues, the major cellular systems involved in the locomotion and physiological functions of animals, have been one of the main sites for verification of miR targets and analysis of their developmental functions. During the determination and differentiation of muscle cells, numerous miRs bind to and repress target mRNAs in a highly specific but redundant manner. Interspecific comparisons of the sequences and expression of miRs have suggested that miR regulation became increasingly important during the course of vertebrate evolution. However, the detailed molecular interactions that have led to the highly complex morphological structures still await investigation. In this review, we will summarize the recent findings on the functional and developmental characteristics of miRs that have played major roles in vertebrate myogenesis, and discuss how the evolution of miRs is related to the morphological complexity of the vertebrates. (C) 2015 Elsevier Ltd. All rights reserved.
    ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, Dec. 2015, SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 47-48, 9 - 16, English
    [Refereed][Invited]
    Introduction scientific journal

  • microRNA経路の中核因子TNRC6Aは複数のメカニズムを介して標的mRNAを抑制する
    三嶋雄一郎, 岸本友良, 深尾亜喜良, 武田耕哲, 坂本博, 藤原俊伸, 井上邦夫
    2010, 生化学

  • Kazuhiro Fukumura, Kunio Inoue
    Last, Informa UK Limited, Sep. 2009, RNA Biology, 6(4) (4), 395 - 398
    [Invited]
    Introduction scientific journal

  • GFP‐nos1‐3′ UTR mRNAによる始原生殖細胞(PGCs)の顕在化とそれを利用した魚類の異種間生殖系列キメラ作出の試み
    斎藤大樹, 吉川智仁, 長井輝美, 大谷哲, 藤本貴史, 相田貴紀, 井上邦夫, 前川真吾, 山羽悦郎
    01 Apr. 2003, 日本水産学会大会講演要旨集, 2003, 130, Japanese

  • mRNA局在化と発生分化制御 (RNAの細胞生物学) -- (高次複合形質とRNA)
    井上 邦夫
    共立出版, Mar. 2003, 蛋白質核酸酵素, 48(4) (4), 451 - 458, Japanese

  • The maintenance of vasa expression has no relevance to the mesoderm induction and axis structure in goldfish(Developmental Biology)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan :
    Otani S., Maegawa S., Inoue K., Arai K., Yamaha E.
    Zoological Society of Japan, 2000, Zoological science, 17, 68 - 68, English

  • スプライシング促進配列を制御することによるエクソンスキッピングの誘導を用いたDuchenne型筋ジストロフィーの治療戦略
    竹島泰弘, 伊東利幸, ZAD P, 坂本博, 井上邦夫, 中村肇, 松尾雅文
    03 Aug. 1999, RNAミーティング, 1st, 33, Japanese

  • PRIMORDIAL GERM CELLS LOCATE IN THE LOWER PART OF BLASTODERM AT THE MID-BLASTULA STAGE IN ZEBRAFISH(Developmental Biology)(Proceedings of the Seventieth Annual Meeting of the Zoological Society of Japan) :
    Nagai T., Otani S., Maegawa S., Inoue K., Arai K., Yamaha E.
    Zoological Society of Japan, 1999, Zoological science, 16, 60 - 60, English

  • Molecular mechanism of germ cell formation in zebrafish
    MAEGAWA S, YASUDA K, INOUE K
    01 Dec. 1998, 日本分子生物学会年会プログラム・講演要旨集, 21(0) (0), Japanese

  • Functional analysis of maternal RNA-binding protein in zebrafish
    SUZUKI H, MAEGAWA S, YASUDA K, INOUE K
    01 Dec. 1998, 日本分子生物学会年会プログラム・講演要旨集, 21(0) (0), Japanese

  • Identification and functional analysis of zebrafish etr gene encoding Elav type RNA connectivity protein.
    NISHIBE TAKAHIRO, SUGIYAMA TOMOYASU, SUZUKI HITOSHI, MAEKAWA SHINGO, TERASHIMA KAZUHIRO, SAKASHITA EIJI, SAKAMOTO HIROSHI, YASUDA KUNIO, INOUE KUNIO
    1998, 日本発生生物学会大会発表要旨集, 31st, 93, Japanese

  • In vitro Analysis of Exon Skipping Induced by Point Mutation in Splicing Enhancer Sequence in Becker Muscular Dystrophy.
    志賀宣之, 竹島泰弘, 坂本博, 井上邦夫, 横田慶之, 横山光宏, 松尾雅文
    Dec. 1997, 日本分子生物学会年会プログラム・講演要旨集, 20th, 685, Japanese

■ Books And Other Publications
  • 熱ストレス応答性のスプライシング制御機構 実験医学 Vol.34-No.19 「coding RNAルネッサンス」 p.3122-p.3126
    INOUE KUNIO
    Joint work, 羊土社, Dec. 2016, Japanese
    Scholarly book

  • microRNAによる初期発生調節機構
    井上 邦夫
    Single work, 蛋白質核酸酵素, Mar. 2007, Japanese
    Others

  • microRNAによるゼブラフィッシュ初期発生制御機構（実験医学）
    三嶋 雄一郎, 井上 邦夫
    Joint work, 羊土社, Mar. 2007, Japanese
    Scholarly book

  • 発生分化過程におけるポリ(A)動態制御（蛋白質核酸酵素・増刊号『RNAと生命』）
    井上 邦夫
    Single work, 共立出版, 2006, Japanese
    Others

  • mRNA局在化の分子機構 （実験医学増刊 Vol.22-No.17、「躍進するRNA研究」p.2545-p.2460
    三嶋 雄一郎, 坂本 博, 井上 邦夫
    Joint work, 羊土社, Nov. 2004, Japanese
    Others

  • RNAスプライシングとゲノム（ゲノム医学）
    井上 邦夫
    Single work, メディカル・レビュー社, 2004, Japanese
    Others

■ Lectures, oral presentations, etc.
  • MafBが制御する心臓神経堤細胞の形成メカニズム
    松花沙織, 卯図優実, 川田結雅, 井上邦夫
    第23回 日本心臓血管発生研究会, Dec. 2024, Japanese, 淡路夢舞台国際会議場, Domestic conference
    Oral presentation

  • Role of CXCR4/SDF1 signaling in the initial migration of cardiac neural crest cells.
    卯図優実, 井上邦夫, 松花沙織
    第47回 日本分子生物学会, Nov. 2024, Japanese, 福岡, Japan, Domestic conference
    Poster presentation

  • The cardiac neural crest gene MafB ectopically directs CXCR4 expression in the trunk neural crest
    Saori Tani-Matsuhana, Yuga Kawata, Kunio Inoue
    第57回 日本発生生物学会, Jun. 2024, English, みやこめっせ/ロームシアター京都, Domestic conference
    Poster presentation

  • ゼブラフィッシュの心臓形成におけるロンボメア5および6由来の心臓神経堤細胞の解析
    阪田 悠世, 松花 沙織, 川上 浩一, 井上 邦夫
    第46回日本分子生物学会年会, Dec. 2023, Japanese, 神戸, Japan, Domestic conference
    Poster presentation

  • 可視光でオン・オフできるGs共役型光遺伝学ツールの開発
    酒寄朗成, 酒井祐輔, 平尚之, 阪田悠世, 小柳光正, 寺北明久, 松花沙織, 井上邦夫, 塚本寿夫
    第61回日本生物物理学会年会, Nov. 2023, English, Domestic conference
    Poster presentation

  • 線虫C. elegansにおいて生殖細胞特異的なTAP様蛋白質NXF-2は新規のRNPを形成しtra-2 3ʼUTR依存的なmRNA核外輸送に必要である
    巳波 孝至, 大谷啓吾, 井上 邦夫, 坂本 博
    関西地区線虫勉強会, Jan. 2023, Japanese, Domestic conference
    Oral presentation

  • The germ cell-specific TAP-like protein NXF-2 forms a novel granular structure and is required for tra-2 3ʼUTR- dependent mRNA export in Caenorhabditis elegans.
    巳波孝至, 大谷啓吾, 井上邦夫, 坂本博
    第45回 日本分子生物学会, Dec. 2022, English, 幕張メッセ, Domestic conference
    Poster presentation

  • Role of MafB gene in cardiac neural crest cell development
    Saori Tani-Matsuhana, Yuga Kawata, Yusei Sakata, Kunio Inoue
    第55回日本発生生物学会, Jun. 2022, English, 金沢市文化ホール, Domestic conference
    Oral presentation

  • 新しい分子機構によってスプライシングされるヒトの短いイントロン群
    福村和宏, 芳本 玲, 廣瀬 哲郎, 井上邦夫, 前田 明
    第44回 日本分子生物学会, Dec. 2021, English, 横浜（ハイブリッド）, Domestic conference
    Poster presentation

  • 線虫C. elegansにおいてクロモドメイン蛋白質MRG-1の阻害はストレス応 答系の活性化に伴う成長遅延を誘導する
    巳波 孝至, 井上 邦夫, 坂本 博
    第44回 日本分子生物学会, Dec. 2021, English, 横浜（ハイブリッド）, Domestic conference
    Poster presentation

  • 遺伝子解析によるヒト体液種同定法の研究
    嵯峨 渉, 井上 邦夫
    第27回 日本法科学技術学会, Nov. 2021, Japanese, オンライン, Domestic conference
    Poster presentation

  • The RNA transport-related factor NXF-2 forms a novel granular structure in C. elegans germ cells
    大谷 啓悟, ⺒波 孝至, 井上 邦夫, 坂本 博
    第22回 日本RNA学会, Jul. 2021, English, オンライン, Domestic conference
    Poster presentation

  • 線虫 C. elegans におけるRNA輸送関連因子NXF-2の機能解析
    大谷 啓悟, ⺒波 孝至, 井上 邦夫, 坂本 博
    線虫勉強会, Jan. 2021, Japanese, Domestic conference
    Oral presentation

  • The RNA transport-related factor NXF-2 forms a novel granular structure in C. elegans germ cells.
    大谷 啓悟, 巳波 孝至, 井上 邦夫, 坂本 博
    第43回日本分子生物学会, Dec. 2020, English, Domestic conference
    Poster presentation

  • A gene regulatory mechanism of early migrating cardiac neural crest cells in chicken embryo
    川田 結雅, 井上 邦夫, 松花(谷) 沙織
    第43回日本分子生物学会, Dec. 2020, English, Domestic conference
    Poster presentation

  • Possible role of RNA secondary structure as thermo sensor regulating alternative splicing
    竹部 真希, 坂本 博, 井上 邦夫
    第43回日本分子生物学会, Dec. 2020, English, Domestic conference
    Poster presentation

  • Control of alternative splicing during recovery period from heat stress.
    樫本 千尋, 逵本 実希, 坂本 博, 井上 邦夫
    第43回日本分子生物学会, Dec. 2020, English, Domestic conference
    Poster presentation

  • SPF45/RBM17 is a novel constitutive splicing factor for a subset of short introns
    Kazuhiro Fukumura, Rei Yoshimoto, Tetsuro Hirose, Kunio Inoue, Akila Mayeda
    第４２回日本分子生物学会, Dec. 2019, English, Domestic conference
    [Invited]
    Public symposium

  • 心臓神経堤細胞の細胞運命を決定する遺伝子制御機構の解析
    村上誠、井上邦夫、松花沙織
    第10回サイエンスフロンティア研究発表会, Oct. 2019, Japanese, 神戸大学大学院理学研究科, Domestic conference
    Poster presentation

  • アミロイドβ線維認識に関わるNLRP3-LRR ドメインの構造特徴と認識機構の研究
    政安 梨緒, 山本 良太, 今村 比呂志, 山本 直樹, MATSUHANA SAORI, INOUE KUNIO, TSUBAKI MOTONARI, CHATANI ERI
    第19回日本蛋白質科学会年会･第71回日本細胞生物学会大会 合同年次大会, Jun. 2019, Japanese, 神戸国際会議場, Domestic conference
    Poster presentation

  • A Distinct Subset of Human Short Introns with Weak Pyrimidine Tract: U2AF Heterodimer Is Replaced by SPF45/RBM17 as General Splicing Factor
    Kazuhiro Fukumura,Rei Yoshimoto,Tetsuro Hirose,Kunio Inoue,Akila Mayeda
    24th Annual Meeting of the RNA Society, Jun. 2019, English, Krakow, Poland, International conference
    [Invited]
    Oral presentation

  • Transcriptome analysis of the cardiac neural crest reveals a critical role for MafB（ポスター）
    Saori Tani-Matsuhana, Kunio Inoue, Marianne Bronner
    第52回日本発生生物学会, May 2019, English, 大阪国際交流センター（大阪）, Domestic conference
    Poster presentation

  • Transcriptome analysis of the cardiac neural crest reveals a critical role for MafB
    Saori Tani-Matsuhana, Kunio Inoue, Marianne Bronner
    第52回日本発生生物学会, May 2019, English, 大阪国際交流センター（大阪）, Domestic conference
    Oral presentation

  • アミロイドβ線維認識に関与するNLRP3-LRRドメインの構造特徴および認識機構の解明
    政安 梨緒, 山本 良太, 今村 比呂志, 山本 直樹, MATSUHANA SAORI, INOUE KUNIO, TSUBAKI MOTONARI, CHATANI ERI
    非共有結合系の分子科学：計測技術から探る生体分子科学の新展開/研究プロジェクト「非共有結合系分子科学研究」, Jan. 2019, Japanese, 神戸大学理学研究科Z201,202, Domestic conference
    Poster presentation

  • 炎症誘起タンパク質NLRP3のLRRドメインによるアミロイドβ線維の直接的認識
    政安 梨緒, 山本 良太, 今村 比呂志, 山本 直樹, MATSUHANA SAORI, INOUE KUNIO, TSUBAKI MOTONARI, CHATANI ERI
    神戸大学 研究基盤センター 若手フロンティア研究会, Dec. 2018, Japanese, 神戸大学百年記念館, Domestic conference
    Poster presentation

  • 線虫C. elegansにおいてクロモドメイン蛋白質MRG-1は始原生殖細胞における転写抑制制御に必要である
    巳波 孝至, 高崎 輝恒, 井上 邦夫, 坂本 博
    第41回日本分子生物学会, Nov. 2018, Japanese, パシフィコ横浜（神奈川県）, Domestic conference
    Poster presentation

  • 核局在性RNA結合タンパク質NONOの細胞内局在制御機構の解析（ポスター）
    古川 真理, 井上 邦夫
    第41回日本分子生物学会, Nov. 2018, Japanese, パシフィコ横浜（神奈川県）, Domestic conference
    Poster presentation

  • 核局在性RNA結合タンパク質NONOの細胞内局在制御機構の解析
    古川 真理, 井上 邦夫
    第41回日本分子生物学会, Nov. 2018, Japanese, パシフィコ横浜（神奈川県）, Domestic conference
    Oral presentation

  • トランスクリプトーム解析により明らかとなった心臓神経堤細胞におけるMafBの重要な機能（ポスター）
    松花(谷) 沙織, 井上 邦夫, Marianne Bronner
    第41回日本分子生物学会, Nov. 2018, Japanese, パシフィコ横浜（神奈川県）, Domestic conference
    Poster presentation

  • トランスクリプトーム解析により明らかとなった心臓神経堤細胞におけるMafBの重要な機能
    松花(谷) 沙織, 井上 邦夫, Marianne Bronner
    第41回日本分子生物学会, Nov. 2018, English, パシフィコ横浜（神奈川県）, Domestic conference
    Oral presentation

  • Identification of Tumor Suppressor RBM4a as a Repressor of Cancer-Specific Mature mRNA Re-splicing
    Toshiki Kameyama, Kazuhiro Fukumura, Masashi Nakatani, Yuta Ohtani, Kunio Inoue, Tetsuro Hirose, Akila Mayeda
    Cold Spring Harbor Asia Conference-RNA Biology, Oct. 2018, English, The Suzhou Dushu Lake Conference Center, Suzhou, China, International conference
    Oral presentation

  • The chromodomain protein MRG-1 is required for global repression of Pol II-dependent transcription in the primordial germ cells in C. elegans
    巳波 孝至, 井上 邦夫, 坂本 博
    線虫研究の未来を創る会, Sep. 2018, Japanese, 国立遺伝学研究所（静岡県）, Domestic conference
    Poster presentation

  • 熱ストレス応答性選択的スプライシング制御機構の解明
    川浪巧, 逵本実希, 坂本博, 井上邦夫
    第20回日本RNA学会年会, Jul. 2018, Japanese, ホテルコスモスクエア（大阪府）, Domestic conference
    Poster presentation

  • Tumor Suppressor Factor RBM4a Represses Cancer-Specific mRNA Re-splicing: A Key Factor for mRNA Quality Control?
    亀山俊樹, 福村和宏, 井上邦夫, 廣瀬哲郎, 前田明
    第20回日本RNA学会年会, Jul. 2018, Japanese, ホテルコスモスクエア（大阪府）, Domestic conference
    Poster presentation

  • U2AFの代わりにSPF45でスプライシングされるヒトの短いイントロン群
    福村和宏, 芳本玲, 廣瀬哲郎, 井上邦夫, 前田明
    第20回日本RNA学会年会, Jul. 2018, Japanese, ホテルコスモスクエア（大阪府）, Domestic conference
    Oral presentation

  • Transcriptome analysis of the cardiac neural crest reveals a critical role for MafB
    Saori Tani-Matsuhana, Felipe Monteleone Vieceli, Shashank Gandhi, Kunio Inoue, Marianne E. Bronner
    Society for Developmental Biology 77th Annual Meeting, Jul. 2018, English, Portland, Oregon, USA, International conference
    Poster presentation

  • Transcriptome analysis of the cardiac neural crest reveals a MAFB gene regulatory subcircuit
    Saori Tani-Matsuhana, Kunio Inoue, Marianne E. Bronner
    第70回日本細胞生物学会、第51回日本発生生物学会合同大会, Jun. 2018, Japanese, タワーホール船堀（東京都）, Domestic conference
    Poster presentation

  • The chromodomain protein MRG-1 is required for global repression of Pol II-dependent transcription in the primordial germ cells in C. elegans
    巳波 孝至, 高崎 輝恒, 井上 邦夫, 坂本 博
    第70回日本細胞生物学会、第51回日本発生生物学会合同大会, Jun. 2018, Japanese, タワーホール船堀（東京都）, Domestic conference
    Poster presentation

  • U2AF is replaced by SPF45 as general splicing factor to recognize weak pyrimidine tract and promotes splicing of human short introns
    Kazuhiro Fukumura, Rei Yoshimoto, Tetsuro Hirose, Kunio Inoue, Akila Mayeda
    The 23th Annual Meeting of the RNA Society, May 2018, English, Berkeley, California, International conference
    Oral presentation

  • 熱ストレス応答性の選択的スプライシング制御機構
    井上 邦夫
    第40回日本分子生物学会年会, Dec. 2017, Japanese, 神戸ポートピアホテル（兵庫県）, Domestic conference
    [Invited]
    Invited oral presentation

  • 線虫クロモドメイン蛋白質MRG-1が始原生殖細胞における転写抑制制御に必要である
    巳波 孝至, 高崎 輝恒, 井上 邦夫, 坂本 博
    第40回日本分子生物学会年会, Dec. 2017, Japanese, 神戸国際展示場2号館（兵庫県）, Domestic conference
    Poster presentation

  • 核局在性RNA結合タンパク質NONOの細胞内局在制御機構と細胞質性顆粒形成機構の解析
    古川 真理, 井上 邦夫
    第40回日本分子生物学会年会, Dec. 2017, Japanese, 神戸国際展示場2号館（兵庫県）, Domestic conference
    Poster presentation

  • メダカにおける胸鰭筋細胞の発生機構
    松花(谷) 沙織, 井上 邦夫
    第40回日本分子生物学会年会, Dec. 2017, Japanese, 神戸国際展示場2号館（兵庫県）, Domestic conference
    Poster presentation

  • ゼブラフィッシュ網膜形成におけるRNA結合タンパク質NonOの機能解析
    宇佐美 知沙, 坂本 博, 井上 邦夫
    第40回日本分子生物学会年会, Dec. 2017, Japanese, 神戸国際展示場2号館（兵庫県）, Domestic conference
    Poster presentation

  • がん抑制因子RBM4aはがん細胞特異的成熟mRNA再スプライシングを抑制する：mRNA品質保証の鍵となる因子か？
    亀山 俊樹, 福村 和宏, 井上 邦夫, 廣瀬 哲郎, 前田 明
    第40回日本分子生物学会年会, Dec. 2017, Japanese, 神戸国際展示場2号館（兵庫県）, Domestic conference
    Oral presentation

  • DND protein functions as a translation repressor during zebrafish embryogenesis
    Kobayashi, M, Tani-Matsuhana, S, Ohkawa, Y, Sakamoto, H, Inoue, K
    第49回日本分子生物学会年会, Dec. 2017, Japanese, 神戸国際展示場2号館（兵庫県）, Domestic conference
    Poster presentation

  • 神経文化に伴うSUMO化修飾状態の変化は、核局在性RNA結合タンパク質NONOの核外移行を誘導する
    古川 真理, 井上 邦夫
    第19回日本RNA学会年会, Jul. 2017, Japanese, 富山国際会議場（富山県）, Domestic conference
    Poster presentation

  • RNPS1は細胞周期に合わせてオーロラキナーゼB遺伝子の正確なスプライシングを制御する
    福村 和宏, 井上 邦夫, 前田 明
    第19回日本RNA学会年会, Jul. 2017, Japanese, 富山国際会議場（富山県）, Domestic conference
    Oral presentation

  • A Repressor Candidate of Cancer Specific mRNA Re-splicing: A Key factor for splicing fidelity or mRNA quality control?
    Toshiki Kameyama, Kazuhiro Fukumura, Kunio Inoue, Tetsuro Hirose, Akila Mayeda
    RNA2017 The 22nd Annual Meeting of the RNA Society, Jun. 2017, English, Prague, Czech Republic, International conference
    Oral presentation

  • Chromodomain protein MRG-1 is required for global repression of Pol II-dependent transcription in the primordial germ cells in C. elegans
    Takashi Miwa, Teruaki Takasaki, Kunio Inoue, Hiroshi Sakamoto
    第50回日本発生生物学会, May 2017, English, タワーホール船堀（東京都）, Domestic conference
    Poster presentation

  • Characterization of RNA-binding protein NonO during retinal development in zebrafish
    Chisa Usami, Hiroshi Sakamoto, Kunio Inoue
    第50回日本発生生物学会, May 2017, English, タワーホール船堀（東京都）, Domestic conference
    Poster presentation

  • The developmental mechanisms of fin muscle cells in medaka, Oryzias latipes
    Saori TANI-MATSUHANA, Kunio Inoue
    第50回日本発生生物学会, May 2017, English, タワーホール船堀（東京都）, Domestic conference
    Poster presentation

  • DND protein functions as a translation repressor during zebrafish embryogenesis
    Kobayashi, M, Tani-Matsuhana, S, Ohkawa, Y, Sakamoto, H, Inoue, K
    第50回日本発生生物学会, May 2017, English, タワーホール船堀（東京都）, Domestic conference
    Poster presentation

  • 熱ストレス応答性の選択的スプライシング制御機構
    井上 邦夫
    第三回バイオシグナル研究会「RNAと生命情報」, Mar. 2017, Japanese, 神戸大学瀧川記念学術交流会館, Domestic conference
    [Invited]
    Invited oral presentation

  • 神経分化に伴って核外移行するRNA結合タンパク質NONOは、SUMO化による細胞内局在制御を受ける
    古川 真理, INOUE KUNIO
    第39回日本分子生物学年会, Nov. 2016, Japanese, パシフィコ横浜, Domestic conference
    Poster presentation

  • 神経細胞におけるRNP顆粒の形成機構の解析
    吉田 篤史, 古川 真理, SAKAMOTO HIROSHI, INOUE KUNIO
    第39回日本分子生物学年会, Nov. 2016, Japanese, パシフィコ横浜, Domestic conference
    Poster presentation

  • 始原生殖細胞の転写抑制状態の維持に関わるクロマチン制御機構の研究
    巳波 孝至, 高崎 輝恒, 井上 邦夫, 坂本 博
    関西地区線虫勉強会, Jun. 2016, Japanese, アプローズタワー 関西学院大学 梅田キャンパス, Domestic conference
    Oral presentation

  • The nuclear RNA binding protein NONO is translocated to the cytoplasm during the neuronal differentiation, which is mediated through deSUMOylation of its specific region by SENP3 and SENP5
    Mari T. Furukawa, Kunio Inoue
    The 2016 joint annual meeting of the RNA Society and the RNA Society of Japan(RNA2016), Jun. 2016, English, Kyoto International Conference Center, International conference
    Poster presentation

  • Chromodomain protein MRG-1 is required for global repression of Pol II-dependent transcription in the primordial germ cells in C. elegans
    Takashi Miwa, Teruaki Takasaki, Kunio Inoue, Hiroshi Sakamoto
    JSDB Special Symposium: Frontier of Developmental Biology Hosted by JSDB, Jun. 2016, English, 東京大学（東京都）, Domestic conference
    Poster presentation

  • 網膜神経節細胞特異的RNA結合タンパク質HERMESはNonO、G3BP1などとともに神経RNA顆粒を形成する
    古川 真理, SAKAMOTO HIROSHI, INOUE KUNIO
    第38回日本分子生物学会, Dec. 2015, Japanese, 神戸国際展示場, Domestic conference
    Oral presentation

  • 線虫C. elegansの生殖細胞形成に必須なクロマチン制御因子群の始原生殖細胞への限局機構の解析
    巳波 孝至, INOUE KUNIO, SAKAMOTO HIROSHI, TAKASAKI TERUAKI
    第38回日本分子生物学会, Dec. 2015, Japanese, 神戸国際展示場, Domestic conference
    Poster presentation

  • 神経細胞におけるRNP顆粒の形成機構の解析
    吉田 篤史, 古川 真理, SAKAMOTO HIROSHI, INOUE KUNIO
    RNA Frontier Meeting 2015, Dec. 2015, Japanese, タカミヤヴィレッジホテル樹林, Domestic conference
    Oral presentation

  • 神経細胞におけるRNP顆粒の形成機構の解析
    吉田 篤史, 古川 真理, SAKAMOTO HIROSHI, INOUE KUNIO
    第38回日本分子生物学会, Dec. 2015, Japanese, 神戸国際展示場, Domestic conference
    Poster presentation

  • ゼブラフィッシュ卵母細胞のmRNA輸送機構の解析
    髙松 陽紀, SAKAMOTO HIROSHI, INOUE KUNIO
    第38回日本分子生物学会, Dec. 2015, Japanese, 神戸国際展示場, Domestic conference
    Poster presentation

  • ゼブラフィッシュ初期胚におけるTob1aの母性mRNA制御機構の解析
    山畑 俊哉, SAKAMOTO HIROSHI, INOUE KUNIO
    第38回日本分子生物学会, Dec. 2015, Japanese, 神戸国際展示場, Domestic conference
    Poster presentation

  • ゼブラフィッシュ初期胚でのTob1aによる母性mRNA制御機構の解析
    山畑 俊哉, SAKAMOTO HIROSHI, INOUE KUNIO
    RNA Frontier Meeting 2015, Dec. 2015, Japanese, タカミヤヴィレッジホテル樹林, Domestic conference
    Oral presentation

  • 線虫C.elegansの始原生殖細胞成熟過程におけるクロマチン制御機構の研究
    巳波 孝至, TAKASAKI TERUAKI, INOUE KUNIO, SAKAMOTO HIROSHI
    関西地区線虫勉強会ジョイントミーティング, Oct. 2015, Japanese, 関西学院大学梅田キャンパス（大阪府）, Domestic conference
    Oral presentation

  • Retinal ganglion cell-specific RNA binding protein HERMES forms the neuronal cytoplasmic RNA granules with NonO and G3BP1
    Mari T Furukawa, Kunio Inoue
    CSHL Meeting on Eukaryotic mRNA Processing, Aug. 2015, English, Cold Spring Harbor Laboratory（アメリカ）, International conference
    Poster presentation

  • 網膜神経節細胞特異的RNA結合タンパク質HERMESはNonO,G3BP1などとともに神経RNA顆粒を形成する
    古川 真理, INOUE KUNIO
    第17回RNA学会年会, Jul. 2015, Japanese, ホテルライフォート札幌（北海道）, Domestic conference
    Oral presentation

  • 線虫C.elegansの生殖細胞形成に必須なクロマチン制御因子群の始原生殖細胞への限局機構の解析
    巳波 孝至, TAKASAKI TERUAKI, INOUE KUNIO, SAKAMOTO HIROSHI
    第17回RNA学会年会, Jul. 2015, Japanese, ホテルライフォート札幌（北海道）, Domestic conference
    Oral presentation

  • ゼブラフィッシュ生殖顆粒構成因子Dead-endにより発現制御を受けるmRNA群の同定
    小林 真奈美, INOUE KUNIO
    第17回RNA学会年会, Jul. 2015, Japanese, ホテルライフォート札幌（北海道）, Domestic conference
    Oral presentation

  • Identification Of MRNAs Regulated By The Germ Granule Protein Dead-end In Zebrafish By RNA-seq.
    小林 真奈美, INOUE KUNIO
    Zebrafish 2015, Jun. 2015, English, Oslo Congress Centre, Norway, International conference
    Oral presentation

  • Identification of mRNAs regulated by the germ granule protein Dead-end in zebrafish by RNA-seq
    小林 真奈美, INOUE KUNIO
    第48回日本発生生物学会, Jun. 2015, Japanese, つくば国際会議場, Domestic conference
    Poster presentation

  • 網膜神経節細胞特異的RNA結合タンパク質HermesはNonOと細胞質でRNP顆粒を形成する
    古川 真理, INOUE KUNIO
    第37回日本分子生物学会, Nov. 2014, Japanese, パシフィコ横浜, Domestic conference
    Poster presentation

  • 線虫C.elegansにおけるクロモドメイン蛋白質MRG-1の始原生殖細胞への限局メカニズムの解析
    巳波 孝至, INOUE KUNIO, SAKAMOTO HIROSHI, TAKASAKI TERUAKI
    第37回日本分子生物学会, Nov. 2014, Japanese, パシフィコ横浜, Domestic conference
    Poster presentation

  • 線虫C.elegansにおけるRNAの輸送経路を決めるメカニズムの解析
    相澤 理丞, INOUE KUNIO, SAKAMOTO HIROSHI
    第37回日本分子生物学会, Nov. 2014, Japanese, パシフィコ横浜, Domestic conference
    Poster presentation

  • ゼブラフィッシュ生殖顆粒構成因子Dead-endにより発現制御を受けるmRNA群の同定と機能解析
    小林 真奈美, INOUE KUNIO
    第37回日本分子生物学会, Nov. 2014, Japanese, パシフィコ横浜, Domestic conference
    Poster presentation

  • ゼブラフィッシュ初期胚におけるTob1aの母性mRNA制御機構の解析
    山畑 俊哉, SAKAMOTO HIROSHI, INOUE KUNIO
    第37回日本分子生物学会, Nov. 2014, Japanese, パシフィコ横浜, Domestic conference
    Poster presentation

  • TNRC6依存的なサイレンシングにおけるデキャッピング促進因子の機能
    牧野 志保, 三嶋 雄一郎, INOUE KUNIO, 稲田 利文
    第37回日本分子生物学会, Nov. 2014, Japanese, パシフィコ横浜, Domestic conference
    Poster presentation

  • 線虫C.elegansにおける、クロモドメイン蛋白質MRG-1の始原生殖細胞への限局メカニズムの解析
    巳波 孝至, TAKASAKI TERUAKI, INOUE KUNIO, SAKAMOTO HIROSHI
    第5回サイエンスフロンティア研究発表会, Oct. 2014, Japanese, 神戸大学, Domestic conference
    Poster presentation

  • 線虫C. elegansにおけるRNAの輸送経路を決める機構の解析
    相澤 理丞, INOUE KUNIO, SAKAMOTO HIROSHI
    関西地区線虫勉強会, Oct. 2014, Japanese, 関西学院大学梅田キャンパス, Domestic conference
    Oral presentation

  • ゼブラフィッシュ初期胚におけるTob1aの母性mRNA制御機構の解析
    山畑 俊哉, SAKAMOTO HIROSHI, INOUE KUNIO
    第5回サイエンスフロンティア研究発表会, Oct. 2014, Japanese, 神戸大学, Domestic conference
    Poster presentation

  • ゼブラフィッシュ生殖質構成因子の同定と機能解析
    小林 真奈美, 金村 節子, INOUE KUNIO
    RNAフロンティアミーティング2014, Sep. 2014, Japanese, ラフォーレ南紀白浜、和歌山県, Domestic conference
    Oral presentation

  • ゼブラフィッシュ初期胚におけるTob1aの母性mRNA制御機構の解析
    山畑 俊哉, SAKAMOTO HIROSHI, INOUE KUNIO
    RNAフロンティアミーティング2014, Sep. 2014, Japanese, ラフォーレ南紀白浜、和歌山県, Domestic conference
    Oral presentation

  • The mechanism for enrichment of a chromodomain protein MRG-1 into the primordial germ cells in C. elegans
    Takashi Miwa, Kunio Inoue, Hiroshi Sakamoto, Teruaki Takasaki
    C. elegans Development, Cell Biology and Gene Expression Meeting in Association with The 6th Asia-Pacific C. elegans Meeting, Jul. 2014, English, 奈良県新公会堂, International conference
    Poster presentation

  • Analysis of the mechanism to enrich a chromodomain protein MRG-1 into the primordial germ cells in C.elegans
    Takashi Miwa, INOUE KUNIO, SAKAMOTO HIROSHI, TAKASAKI TERUAKI
    第47回日本発生生物学会, May 2014, Japanese, ウインクあいち、愛知県名古屋市, Domestic conference
    Oral presentation

  • Developmental roles and evolution of the bicistronic microRNA precursor, miR-1/miR-133, expressed specifically in muscle tissues / 筋肉特異的microRNA前駆体miR-1/miR-133の機能と進化
    Rie Kusakabe, Saori Tani, Kunio Inoue
    第47回日本発生生物学会, May 2014, English, ウインクあいち、愛知県名古屋市, Domestic conference
    Oral presentation

  • Analysis of the mechanism to enrich a chromodomain protein MRG-1 into the primordial germ cells in C. elegans
    Takashi Miwa, Kunio Inoue, Hiroshi Sakamoto, Teruaki Takasaki
    第47回日本発生生物学会, May 2014, English, ウインクあいち、愛知県名古屋市, Domestic conference
    Poster presentation

  • 線虫C.elegans生殖細胞におけるスプライシング関連因子SPK-1の機能解析
    土橋 匠, TAKASAKI TERUAKI, INOUE KUNIO, SAKAMOTO HIROSHI
    関西地区線虫勉強会ジョイントミーティング, Oct. 2013, Japanese, 関西学院大学梅田キャンパス, Domestic conference
    Oral presentation

  • ゼブラフィッシュ生殖質構成因子の機能解析
    小林 真奈美, INOUE KUNIO
    サイエンスフロンティア研究発表会, Oct. 2013, Japanese, 神戸大学, Domestic conference
    Oral presentation

  • 線虫C.elegans生殖細胞におけるスプライシング関連タンパク質リン酸化酵素SPK-1の機能解析
    土橋 匠, TAKASAKI TERUAKI, INOUE KUNIO, SAKAMOTO HIROSHI
    RNAフロンティアミーティング2013, Sep. 2013, Japanese, ラフォーレ修善寺, Domestic conference
    Oral presentation

  • 線虫C.elegansにおける生殖細胞形成に関与するクロマチンリモデリング因子群の局在機構の解析
    巳波 孝至, TAKASAKI TERUAKI, INOUE KUNIO, SAKAMOTO HIROSHI
    RNAフロンティアミーティング, Sep. 2013, Japanese, ラフォーレ修善寺, Domestic conference
    Oral presentation

  • 線虫C.elegansにおけるRNAの輸送経路を決める機構の解析
    相澤 理丞, INOUE KUNIO, SAKAMOTO HIROSHI
    RNAフロンティアミーティング2013, Sep. 2013, Japanese, ラフォーレ修善寺, Domestic conference
    Oral presentation

  • The regulatory mechanisms of apoptosis by Hermes, the retinal ganglion cell specific RNA binding protein
    Mari Furukawa, Kunio Inoue
    RNA Frontier Meeting 2013, Sep. 2013, English, ラフォーレ修善寺, Domestic conference
    Oral presentation

  • The regulatory mechanism of apoptosis by Hermes, the retinal ganglion cell specific RNA binding protein
    Mari Furukawa, Kunio Inoue
    RiboClub, 14th annual meeting, Sep. 2013, English, Hotel Cheribourg, Quebec, Canada, International conference
    Poster presentation

  • 網膜神経節細胞特異的RNA結合タンパク質Hermesによるアポトーシス制御機構の解析
    古川 真理, INOUE KUNIO
    第15回日本RNA学会年会, Jul. 2013, Japanese, 愛媛県県民文化会館ひめぎんホール, Domestic conference
    Oral presentation

  • EVOLUTION OF REGULATORY MECHANISMS IN HYPAXIAL MUSCLE DEVELOPMENT
    Rie Kusakabe, Saori Tani, Richard, M. Harland, Kunio Inoue, Shigeru Kuratani
    10th International Congress of Vertebrate Morphology, Jul. 2013, English, Hotel Fira Palace, Barcelona, Spain, International conference
    Oral presentation

  • The gene regulation in skeletal myogenesis in medaka, Oryzias latipes.
    Saori Tani, Rie Kusakabe, Kunio Inoue
    17th International Congress of Developmental Biology, Jun. 2013, English, Cancun,Mexico, International conference
    Oral presentation

  • Roles of microRNAs in medaka myogenesis
    Saori Tani, Rie Kusakabe, Kunio Inoue
    第46回日本発生生物学会, May 2013, English, くにびきメッセ, Domestic conference
    Oral presentation

  • Developmental evolution of the hypaxial muscles of vertebrates
    Rie Kusakabe, Saori Tani, Richard M. Harland, Shigeru Kuratani, Kunio Inoue
    第46回日本発生生物学会, May 2013, English, くにびきメッセ, Domestic conference
    Oral presentation

  • 線虫EJC中核因子Y１４はスプライシング未完了RNAの核外輸送を防ぐ
    椎森 仁美, INOUE KUNIO, SAKAMOTO HIROSHI
    特定領域研究「RNA制御学」班会議, Jan. 2013, Japanese, レオパレス仙台, Domestic conference
    Oral presentation

  • ゼブラフィッシュ生殖顆粒構成因子Granulitoタンパク質の機能解析
    稲川 陽介, 金村 節子, 三嶋 雄一郎, 柴田 英明, 前川 真吾, SAKAMOTO HIROSHI, INOUE KUNIO
    特定領域研究「RNA制御学」班会議, Jan. 2013, Japanese, レオパレス仙台, Domestic conference
    Poster presentation

  • ゼブラフィッシュ胚発生を制御microRNAシステムの分子基盤
    三嶋雄一郎, 星島 一幸, David Grunwald, INOUE KUNIO
    第３５回分子生物学会年会・ワークショップ「RNA制御の細胞生物学」, Dec. 2012, Japanese, 福岡国際会議場, Domestic conference
    Oral presentation

  • ゼブラフィッシュ生殖顆粒構成因子Granulitoタンパク質の機能解析
    稲川 陽介, 金村 節子, 三嶋 雄一郎, 柴田 英明, 前川 真吾, SAKAMOTO HIROSHI, INOUE KUNIO
    第35回分子生物学会年会, Dec. 2012, Japanese, 福岡国際会議場, Domestic conference
    Poster presentation

  • RNA regulation in zebrafish germ cell formation.
    Inoue Kunio
    2012 Taiwan-Japan Joint Symposium on cell signaling and gene regulation., Nov. 2012, English, 国立成功大学、台南, International conference
    Invited oral presentation

  • ゼブラフィッシュ生殖顆粒構成因子Granulitoタンパク質の機能解析
    稲川 陽介, 金村 節子, 三嶋 雄一郎, 前川 真吾, SAKAMOTO HIROSHI, INOUE KUNIO
    RNAフロンティアミーテイング2012, Sep. 2012, Japanese, メルパルク熊本, Domestic conference
    Poster presentation

  • Exon Junction Complexは細胞周期関連遺伝子の効率的なpre-mRNAスプライシングに寄与する
    福村 和宏, 若林 俊一, 河村 有砂, SAKAMOTO HIROSHI, 鈴木 穣, 中井 謙太, INOUE KUNIO
    RNAフロンティアミーティング2012, Sep. 2012, Japanese, メルパルク熊本, Domestic conference
    Poster presentation

  • 線虫EJC中核因子Y１４はスプライシング未完了RNAの核外輸送を防ぐ
    椎森 仁美, INOUE KUNIO, SAKAMOTO HIROSHI
    第14回日本RNA学会年会, Jul. 2012, Japanese, 東北大学百周年記念会館, Domestic conference
    Oral presentation

  • Functional analysis of a germ granule component, Granulito, in the zebrafish germplasm
    稲川 陽介, 金村 節子, 三嶋 雄一郎, 前川 真吾, SAKAMOTO Hiroshi, INOUE Kunio
    第14回日本RNA学会年会, Jul. 2012, Japanese, 東北大学百周年記念会館, Domestic conference
    Poster presentation

  • Molecular Mechanisms of MicroRNA-mediated Gene Silencing during Zebrafish Embryogenesis.
    Yuichiro Mishima, Kunio Inoue
    The 22nd CDB Meeting - RNA Sciences in Cell and Developmental Biology II, Jun. 2012, English, 理化学研究所CDB, International conference
    Oral presentation

  • EJC core subunits have a novel gatekeeper function in mRNA quality control in the nematode C. elegans．
    Masami Shiimori, Kunio Inoue, Hiroshi Sakamoto
    The 22th CDB Meeting - RNA Sciences in Cell and Developmental Biology II, Jun. 2012, English, 理化学研究所CDB（神戸）, International conference
    Oral presentation

  • 熱ストレスに応答した選択的スプライシング制御機構の解析
    河村 有砂, 山田 智子, FUKUMURA KAZUHIRO, 鈴木 仁, Vu, Luyen Thi, 塚原 俊文, SAKAMOTO Hiroshi, INOUE Kunio
    第34回日本分子生物学会年会, Dec. 2011, Japanese, 日本分子生物学会, 横浜, Domestic conference
    Poster presentation

  • Molecular mechanisms and regulations of microRNA-mediated gene silencing in zebrafish
    MISHIMA Yuichiro, INOUE Kunio
    第34回日本分子生物学会年会, Dec. 2011, English, 日本分子生物学会, 横浜, Domestic conference
    Oral presentation

  • Functional analysis of an RNP component, Granulito, in the zebrafish germplasm
    稲川 陽介, 金村 節子, MISHIMA Yuichiro, 前川 真吾, SAKAMOTO Hiroshi, INOUE Kunio
    第34回日本分子生物学会年会, Dec. 2011, English, 日本分子生物学会, 横浜, Domestic conference
    Poster presentation

  • 選択的スプライシング制御とその破綻
    INOUE Kunio
    内分泌疾患談話会, Nov. 2011, Japanese, 群馬, Domestic conference
    Invited oral presentation

  • Expression and function of muscle-specific microRNAs in medaka
    Saori Tani, Rie Kusakabe, Kiyoshi Naruse, Hiroshi Sakamoto, Kunio Inoue
    International Symposium Genetic Regulation of Development, Nov. 2011, English, 京都, Domestic conference
    Poster presentation

  • 線虫Y14はイントロンを含む未スプライシングRNAの核外漏出を防ぐ
    椎森 仁美, FUKUMURA KAZUHIRO, 若林 俊一, 鈴木 穣, 中井 謙太, INOUE Kunio, SAKAMOTO Hiroshi
    第84回日本生化学会大会, Sep. 2011, Japanese, 日本生化学会, 京都, Domestic conference
    Invited oral presentation

  • メダカの神経系発生過程におけるmiR-124の機能
    KATO Yumiko, KUSAKABE Rie, INOUE Kunio, TOCHINAI Shin
    第82回日本動物学会, Sep. 2011, Japanese, 日本動物学会, 旭川, Domestic conference
    Poster presentation

  • Expression and function of muscle-specific microRNAs in medaka
    Saori Tani, Rie Kusakabe, Kiyoshi Naruse, Hiroshi Sakamoto, Kunio Inoue
    第17回小型魚類研究会, Sep. 2011, English, 小型魚類研究会, 三島, Domestic conference
    Oral presentation

  • 線虫Y14はイントロンを含む未スプライシングRNAの核外輸送を防ぐ
    椎森 仁美, INOUE Kunio, SAKAMOTO Hiroshi
    RNAフロンティアミーティング2011, Aug. 2011, Japanese, 愛知, Domestic conference
    Oral presentation

  • Two EJC core subunits, Y14 and MAG-1, have a novel gatekeeper function in mRNA quality control in the nematode C. elegans
    Masami Shiimori, Shunichi Wakabayashi, Yutaka Suzuki, Kenta Nakai, Kunio Inoue, Hiroshi Sakamoto
    RNA2011, Jun. 2011, English, The RNA Society, 日本RNA学会, 京都, Domestic conference
    Oral presentation

  • Molecular mechanisms of miRNA-mediated repression during zebrafish embryogenesis
    Yuichiro Mishima, Akira Fukao, Tomoyoshi Kishimoto, Hiroshi Sakamoto, Toshinobu Fujiwara, Kunio Inoue
    RNA2011, Jun. 2011, English, The RNA Society, 日本RNA学会, 京都, Domestic conference
    Poster presentation

  • Role of miR-124 in neural development of medaka, Oryzias latipesメダカの神経系発生過程におけるmiR-124の機能
    Yumiko Kato, Rie Kusakabe, Kunio Inoue, Shin Tochinai
    44th Annual Meeting for the Japanese Society of Developmental Biologists, May 2011, English, 日本発生生物学会, 沖縄, Domestic conference
    Poster presentation

  • Developmental roles of muscle-specific microRNAs in medakaメダカ胚における筋肉特異的microRNAの発現と機能
    Saori Tani, Rie Kusakabe, Kiyoshi Naruse, Hiroshi Sakamoto, Kunio Inoue
    44th Annual Meeting for the Japanese Society of Developmental Biologists, May 2011, English, 日本発生生物学会, 沖縄, Domestic conference
    Oral presentation

  • Molecular mechanisms of miRNA-mediated repression during zebrafish embryogenesis
    Yuichiro Mishima, Tomoyoshi Kishimoto, Akira Fukao, Hiroshi Sakamoto, Toshinobu Fujiwara, Kunio Inoue
    Keystone Symposia on Molecular and Cellular Biology “Mechanisms and Biology of RNA silencing, Mar. 2011, English, Keystone Symposia, California, USA, International conference
    Poster presentation

  • 線虫C. elegansの生殖細胞性決定機構におけるY14の機能解析
    椎森 仁美, INOUE Kunio, SAKAMOTO Hiroshi
    第33回日本分子生物学会年会, Dec. 2010, Japanese, 日本分子生物学会, 神戸, Domestic conference
    Poster presentation

  • メダカ胚における筋肉特異的microRNAの発現と機能
    TANI Saori, KUSAKABE Rie, NARUSE Kiyoshi, SAKAMOTO Hiroshi, INOUE Kunio
    第33回日本分子生物学会年会, Dec. 2010, Japanese, 日本分子生物学会, 神戸, Domestic conference
    Poster presentation

  • miRISC中核因子であるTNRC6Aタンパク質によるポリＡ鎖短縮機構の解析
    岸本 友良, MISHIMA YUICHIRO, SAKAMOTO Hiroshi, INOUE Kunio
    第33回日本分子生物学会年会, Dec. 2010, Japanese, 日本分子生物学会, 神戸, Domestic conference
    Poster presentation

  • microRNA経路の中核因子TNRC6Aは複数のメカニズムを介して標的mRNAを抑制する
    MISHIMA Yuichiro, 岸本 友良, 深尾 亜喜良, 武田 耕哲, SAKAMOTO Hiroshi, FUJIWARA Toshinobu, INOUE Kunio
    第33回日本分子生物学会年会, Dec. 2010, Japanese, 日本分子生物学会, 神戸, Domestic conference
    Oral presentation

  • ゼブラフィッシュ生殖細胞形成に働くRNA情報発現制御システム
    INOUE Kunio
    北陸先端科学技術大学院大学マテリアルサイエンス研究科セミナー, Nov. 2010, Japanese, 北陸先端科学技術大学院大学マテリアルサイエンス研究科, 石川, Domestic conference
    Invited oral presentation

  • メダカの神経系発生過程におけるmiR-124の機能
    KATO Yumiko, KUSAKABE Rie, INOUE Kunio, TOCHINAI Shin
    日本動物学会第81回大会, Sep. 2010, Japanese, 日本動物学会, 東京, Domestic conference
    Oral presentation

  • カタユウレイボヤ脳胞におけるpiwiの新規機能の解析
    島井 光太郎, 一瀬 葵, 宮本 由紀, 寺島 泰子, 西辻 光希, 白江ー倉林, 麻, 中村 輝, KUSAKABE RIE, 中井 謙太, INOUE Kunio, 日下部 岳広
    日本動物学会第81回大会, Sep. 2010, Japanese, 日本動物学会, 東京, Domestic conference
    Oral presentation

  • Functional interaction between the neuronal RNA-binding protein HuD and Akt1
    Akira Fukao, Yumi Sasano, Hiroaki Imataka, Kunio Inoue, Hiroshi Sakamoto, Nahum Sonenberg, Christian Thoma, Toshinobu Fujiwara
    TRANSLATIONAL CONTROL, Sep. 2010, English, CSHL, New York, USA, International conference
    Poster presentation

  • microRNA経路の中核因子TNRC6Aは複数のメカニズムを介して標的mRNAを抑制する
    MISHIMA Yuichiro, 岸本 友良, 深尾 亜喜良, 武田 耕哲, SAKAMOTO Hiroshi, FUJIWARA TOSHINOBU, INOUE Kunio
    第2回RNAi研究会, Aug. 2010, Japanese, RNAi研究会, 広島, Domestic conference
    Oral presentation

  • Control of germline/somatic cell distinction by miRNA function in zebrafish
    INOUE Kunio
    A satellite symposium on Germ Cells in SDB-JSDB Joint Meeting 2010, Aug. 2010, English, SDB, JSDB, New Mexico, USA, International conference
    Invited oral presentation

  • 線虫C. elegansの生殖細胞性決定機構におけるY14の機能解析
    椎森 仁美, INOUE Kunio, SAKAMOTO Hiroshi
    第12回RNAミーティング（日本RNA学会年会）, Jul. 2010, Japanese, 日本RNA学会, 東京, Domestic conference
    Oral presentation

  • 神経特異的RNA結合蛋白質HuDとシグナル伝達因子Aktとの連携による翻訳制御機構
    深尾 亜喜良, 笹野 有未, 今高 寛晃, INOUE Kunio, SAKAMOTO Hiroshi, Nahum Sonenberg, Christian Thoma, 藤原 俊伸
    第12回RNAミーティング（日本RNA学会年会）, Jul. 2010, Japanese, 日本RNA学会, 東京, Domestic conference
    Oral presentation

  • Role of Y14 in C. elegans germline sexual differentiation
    椎森 仁美, INOUE Kunio, SAKAMOTO Hisroshi
    4th East Asia C. elegans Meeting, Jul. 2010, English, 東京, Domestic conference
    Poster presentation

  • The Molecular Mechanisms of TNRC6 to Induce Translational Repression and Deadenylation in Zebrafish
    Yuichiro Mishima, Akira Fukao, Hiroshi Sakamoto, Toshinobu Fujiwara, Kunio Inoue
    The 19th CDB Meeting-RNA Sciences in Cell and Developmental Biology, May 2010, English, CDB, 神戸, Domestic conference
    Invited oral presentation

  • Role of Y14 in C. elegans germline sexual differentiation
    椎森 仁美, INOUE Kunio, SAKAMOTO Hiroshi
    The 19th CDB Meeting-RNA Sciences in Cell and Developmental Biology, May 2010, English, CDB, 神戸, Domestic conference
    Poster presentation

  • Genomic organization, expression and evolution of muscle-specific microRNAs
    Rie Kusakabe, Saori Tani, Shigehiro Kuraku, Yuki Miyamoto, Kohji Okamura, Kenta Nakai, Takehiro G. Kusakabe, Kunio Inoue
    The 19th CDB Meeting-RNA Sciences in Cell and Developmental Biology, May 2010, English, CDB, 神戸, Domestic conference
    Poster presentation

  • メダカ初期胚におけるmiR-430の発現と機能
    TANI Saori, KUSAKABE Rie, NARUSE Kiyoshi, SAKAMOTO Hiroshi, INOUE Kunio
    第1回 甲南大学・モレキュラーサイエンスワークショップ, Mar. 2010, Japanese, 甲南大学, 神戸, Domestic conference
    [Invited]
    Invited oral presentation

  • ゼブラフィッシュmiRNA制御システムによる生殖細胞・体細胞の分化機構
    INOUE Kunio
    第1回 甲南大学・モレキュラーサイエンスワークショップ, Mar. 2010, Japanese, 甲南大学, 神戸, Domestic conference
    Invited oral presentation

  • The Molecular Mechanisms of TNRC6 to Induce Translational Repression and Deadenylation in Zebrafish
    Yuichiro Mishima, Akira Fukao, Hiroshi Sakamoto, Toshinobu Fujiwara, Kunio Inoue
    EMBL Conference: The complex life of mRNA: From synthesis to decay, Mar. 2010, English, EMBL, ドイツ, International conference
    Poster presentation

  • RNAスプライシングによる形質発現制御機構
    INOUE Kunio
    第8回 神戸膠原病研究会, Mar. 2010, Japanese, 神戸膠原病研究会, 神戸, Domestic conference
    Invited oral presentation

  • Control of germline/somatic cell distinctions by miRNA function
    INOUE Kunio
    The NIBB International Practical course “Developmental Genetics of zebrafish and medaka”, Jan. 2010, English, 基礎生物学研究所, 岡崎, Domestic conference
    Invited oral presentation

  • メダカ初期胚におけるmiR-430の発現と機能
    TANI Saori, KUSAKABE Rie, NARUSE Kiyoshi, SAKAMOTO Hiroshi, INOUE Kunio
    第32回日本分子生物学会年会, Dec. 2009, Japanese, 日本分子生物学会, 神奈川, Domestic conference
    Poster presentation

  • U1-independent splicing and the splicing regulation
    INOUE Kunio
    第32回日本分子生物学会年会, Dec. 2009, English, 日本分子生物学会, 神奈川, Domestic conference
    Invited oral presentation

  • Role of Y14 in C. elegans germline sexual differentiation
    椎森 仁美, INOUE Kunio, SAKAMOTO Hiroshi
    第32回日本分子生物学会年会, Dec. 2009, English, 日本分子生物学会, 神奈川, Domestic conference
    Poster presentation

  • Regulation of vertebrate gene expression by microRNAs
    Yuichiro Mishima, Antonio J. Giraldez, Kunio Inoue
    第32回日本分子生物学会年会, Dec. 2009, English, 日本分子生物学会, 神奈川, Domestic conference
    Invited oral presentation

  • Role of Y14 in C. elegans germline sexual differentiation
    椎森 仁美, INOUE Kunio, SAKAMOTO Hiroshi
    2009Japan-Taiwan Joint Symposium on Cell Signaling and Gene Regulation, Nov. 2009, English, 神戸, International conference
    Poster presentation

  • 線虫C. elegansの生殖細胞性決定機構におけるY14の機能解析
    椎森 仁美, INOUE Kunio, SAKAMOTO Hiroshi
    RNAフロンティアミーティング2009, Sep. 2009, Japanese, 神奈川, Domestic conference
    Oral presentation

  • 筋肉ではたらくmicroRNAの機能と進化
    KUSAKABE Rie, 谷 沙織, 工樂 樹洋, 成瀬 清, 宮本 由紀, 岡村 浩司, 中井 健太, 日下部 岳広, INOUE Kunio
    第15回小型魚類研究会, Sep. 2009, Japanese, 小型魚類研究会, 名古屋, Domestic conference
    Oral presentation

  • メダカ初期胚におけるmiR-430の発現と機能
    TANI Saori, KUSAKABE Rie, NARUSE Kiyoshi, SAKAMOTO Hiroshi, INOUE Kunio
    第15回小型魚類研究会, Sep. 2009, Japanese, 小型魚類研究会, 名古屋, Domestic conference
    Poster presentation

  • Evolution of vertebrate myogenesis; with special reference to the morphological variety of skeletal muscles
    Rie Kusakabe, Shigehiro Kuraku, Shigeru Kuratani, Kunio Inoue
    International Society for Developmental Biology, Sep. 2009, English, ISDB, Edinburgh, International conference
    Poster presentation

  • Enhancement of cap-dependent translation by the ELAV protein HuD: A novel function of HuD which is eIF4A- and poly(A)-dependent
    Akira Fukao, Yumi Sasano, Hiroaki Imataka, Kunio Inoue, Hiroshi Sakamoto, Nahum Sonenberg, Christian Thoma, Toshinobu Fujiwara
    Protein Synthesis and Translational Control, Sep. 2009, English, EMBL, Heidelberg, Germany, International conference
    Oral presentation

  • 線虫C. elegansの生殖細胞性決定機構におけるY14の機能解析
    椎森 仁美, INOUE Kunio, SAKAMOTO Hiroshi
    第11回 RNAミーティング, Jul. 2009, Japanese, 日本RNA学会, 新潟, Domestic conference
    Poster presentation

  • Zebrafish Dazl ensures PGC specific gene expression through blocking the miRNA-mediated gene silencing
    Yasuaki Takeda, Yuichiro Mishima, Hiroshi Sakamoto, Kunio Inoue
    6th European Zebrafish Genetics and Development Meeting, Jul. 2009, English, Rome, Italy, International conference
    Poster presentation

  • poly(A)配列およびeIF4Aを介したELAV蛋白質HuDによるcap依存的翻訳促進機構
    FUKAO Akira, SASANO Yumi, IMATAKA Hiroaki, INOUE Kunio, SAKAMOTO Hiroshi, Nahum Sonenberg, Christian Thoma, FUJIWARA Toshinobu
    第11回 RNAミーティング, Jul. 2009, Japanese, 日本RNA学会, 新潟, Domestic conference
    Oral presentation

  • Genomic organization and developmental expression of tunicate microRNAs
    谷 沙織, KUSAKABE Rie, 宮本 由紀, 岡村 浩司, 中井 健太, 日下部 岳広, INOUE Kunio
    The 5th International Tunicate meeting, Jun. 2009, English, 沖縄, Domestic conference
    [Invited]
    Invited oral presentation

  • Functional analysis of Y14 in C. elegans germline sexual differentiation
    椎森 仁美, 郷原 美里, 笹野 有未, INOUE Kunio, SAKAMOTO Hiroshi
    第42回日本発生生物学会大会, May 2009, English, 日本発生生物学会, 新潟, Domestic conference
    Poster presentation

  • Evolution and developmental expression of chordate microRNAs
    Rie Kusakabe, Saori Tani, Shigehiro Kuraku, Yuki Miyamoto, Kohji Okamura, Kenta Nakai, Takehiro G. Kusakabe, Kunio Inoue
    第42回日本発生生物学会大会, May 2009, English, 日本発生生物学会, 新潟, Domestic conference
    Oral presentation

  • Embryonic expression and function of miR-430 in medaka
    Saori Tani, Rie Kusakabe, Kiyoshi Naruse, Hiroshi Sakamoto, Kunio Inoue
    第42回日本発生生物学会大会, May 2009, English, 日本発生生物学会, 新潟, Domestic conference
    Oral presentation

  • ゼブラフィッシュGerm plasm RNAの局在化機構の解析
    森 さやか, SAKAMOTO Hiroshi, INOUE Kunio
    第31回日本分子生物学会年会、第81回生化学会大会, Dec. 2008, Japanese, 日本分子生物学会, 神戸, Domestic conference
    Poster presentation

  • U1snRNP非依存的pre-mRNAスプライシングは選択的スプライシング制御に寄与する
    福村 和宏, 谷口 一郎, 新名主 カオリ, SAKAMOTO Hiroshi, 大野 睦人, INOUE Kunio
    第31回日本分子生物学会年会、第81回生化学会大会, Dec. 2008, Japanese, 日本分子生物学会, 神戸, Domestic conference
    Oral presentation

  • U1-independent pre-mRNA splicing contributes to the regulation of alternative splicing
    INOUE Kunio
    東京医科歯科大学難治疾患研究所病態発現機構研究部門主催国際シンポジウム, Dec. 2008, English, 東京医科歯科大学難治疾患研究所病態発現機構研究部門, 東京, Domestic conference
    Invited oral presentation

  • DAZLによるPGC特異的遺伝子-TDRD7に働くmiRNA機能の解除
    武田 耕哲, MISHIMA Yuichiro, FUJIWARA Toshinobu, SAKAMOTO Hiroshi, INOUE Kunio
    第31回日本分子生物学会年会、第81回生化学会大会, Dec. 2008, Japanese, 日本分子生物学会, 神戸, Domestic conference
    Oral presentation

  • NEURONAL HUD PROTEINS ASSOCIATE WITH EIF4F AND DIRECTLY RECRUIT CATALYTICALLY ACTIVE AKT1 TO INDUCE NEURONAL DIFFERENTIATION
    Akira Fukao, Christian Thoma, Kunio Inoue, Hiroaki Imataka, Nahum Sonenberg, Hiroshi Sakamoto, Toshinobu Fujiwara
    TRANSLATIONAL CONTROL, Sep. 2008, English, CSHL, New York, USA, International conference
    Poster presentation

  • ゼブラフィッシュ生殖細胞形成とRNA生物学
    INOUE Kunio
    第3回生殖研究ワークショップ, Aug. 2008, Japanese, 神奈川, Domestic conference
    Invited oral presentation

  • U1 snRNP非依存的pre-mRNAスプライシング分子機構の研究
    福村 和宏, 谷口 一郎, 新名主 カオリ, SAKAMOTO Hiroshi, 大野 睦人, INOUE Kunio
    第10回日本RNA学会年会, Jul. 2008, Japanese, 日本RNA学会, 北海道, Domestic conference
    Oral presentation

  • The mechanism blocking the miRNA-mediated silencing of PGC-specific TDRD7 gene in zebrafish
    Yasuaki Takeda, Yuichiro Mishima, Hiroshi Sakamoto, Kunio Inoue
    13th Annual Meeting of the RNA Society, Jul. 2008, English, The RNA Society, Berlin,Germany, International conference
    Poster presentation

  • Differential gene expression of muscle-related genes in the lamprey and teleosts
    Rie Kusakabe, Shigehiro Kuraku, Kunio Inoue, Shigeru Kuratani
    The second meeting of European Society for Evolutionary Developmental Biology, Jul. 2008, English, Gent,Belgium, International conference
    Oral presentation

  • 線虫C. elegansの生殖細胞性決定機構におけるY14の機能解析
    椎森 仁美, 笹野 有未, 郷原 美里, INOUE Kunio, SAKAMOTO Hiroshi
    RNAフロンティアミーティング2008, Jun. 2008, Japanese, 京都, Domestic conference
    Oral presentation

  • 神経特異的RNA結合タンパク質HuDによる翻訳制御機構
    FUKAO Akira, IMATAKA Hiroaki, INOUE Kunio, Nahum Sonenberg, SAKAMOTO Hiroshi, FUJIWARA Toshinobu
    RNAフロンティアミーティング2008, Jun. 2008, Japanese, 京都, Domestic conference
    Oral presentation

  • U1に依存しないスプライシングによる制御機構
    INOUE Kunio
    東京医科歯科大学大学院疾患生命科学研究部・形質発現セミナー, Jun. 2008, Japanese, 東京医科歯科大学大学院疾患生命科学研究部, 東京, Domestic conference
    Invited oral presentation

  • U1に依存しないスプライシングによる制御機構
    INOUE Kunio
    京都大学ウイルス研究所セミナー, Jun. 2008, Japanese, 京都大学ウイルス研究所, 京都, Domestic conference
    Invited oral presentation

  • ゼブラフィッシュ生殖細胞遺伝子TDRD7のmiRNAによる抑制はDazlによって解除される
    武田 耕哲, MISHIMA Yuichiro, SAKAMOTO Hiroshi, INOUE Kunio
    第41回日本発生生物学会・サテライトワークショップ「多分野で活躍する小型魚類」, May 2008, Japanese, 日本発生生物学会, 徳島, Domestic conference
    Poster presentation

  • ゼブラフィッシュmiRNA制御システムによる生殖細 胞形成機構
    INOUE Kunio
    第41回日本発生生物学会・サテライトワークショップ「多分野で活躍する小型魚類」, May 2008, Japanese, 日本発生生物学会, 徳島, Domestic conference
    Invited oral presentation

  • Differential expression and molecular interaction of regulatory genes in vertebrate myogenesis脊椎動物の骨格筋発生過程における遺伝子制御機構
    Rie Kusakabe, Shigehiro Kuraku, Kunio Inoue, Shigeru Kuratani
    第41回日本発生生物学会・サテライトワークショップ「多分野で活躍する小型魚類」, May 2008, English, 日本発生生物学会, 徳島, Domestic conference
    Poster presentation

  • Molecular mechanism of alternative splicing regulation by RNA binding protein Fox-1
    福村 和宏, 加藤 彩子, 神 唯, FUJIWARA TOSHINOBU, SAKAMOTO Hiroshi, INOUE Kunio
    日本分子生物学会・日本生化学会, Dec. 2007, English, 日本分子生物学会, 横浜, Domestic conference
    Invited oral presentation

  • miRNA制御システムによる生殖細胞・体細胞分化確立機構
    INOUE Kunio
    日本分子生物学会・日本生化学会, Dec. 2007, Japanese, 日本分子生物学会, 横浜, Domestic conference
    Invited oral presentation

  • ゼブラフィッシュ生殖細胞形成過程に働くRNAプログラム
    INOUE Kunio
    日本発生生物学会 秋季シンポジウム, Nov. 2007, Japanese, 日本発生生物学会, 岡崎, Domestic conference
    Invited oral presentation

  • 小さなRNAの大きな役割～microRNAによるゼブラフィッシュ初期発生 調節機構～
    INOUE Kunio
    和光純薬セミナー, Oct. 2007, Japanese, 和光純薬, 大阪, Domestic conference
    Invited oral presentation

  • ゼブラフィッシュGermplasm RNAの局在化機構の解析
    森 さやか, 小坂 恭子, SAKAMOTO Hiroshi, INOUE Kunio
    RNA研究若手の会, Sep. 2007, Japanese, 神戸, Domestic conference
    Oral presentation

  • Neuronal RNA binding protein HuD interact with protein kinase B
    FUKAO Akira, TAKEUCHI Akiko, FUJIWARA Toshinobu, MATSUZAKI Hidenori, INOUE Kunio, KIKKAWA Ushio, SAKAMOTO Hiroshi
    EMBO Conference on Protein Synthesis and Translational Control, Sep. 2007, English, European Molecular Biology Laboratory, Heidelberg, Germany, International conference
    Poster presentation

  • 小さなRNAの大きな役割〜microRNAによるゼブラフィッシュ初期発生調節機構 〜
    INOUE Kunio
    第5回最先端動物遺伝育種セミナー, Aug. 2007, Japanese, 日本動物遺伝育種学会, 山梨, Domestic conference
    Invited oral presentation

  • ゼブラフィッシュ生殖細胞形成過程に働くRNAプログラム
    INOUE Kunio
    第9回日本RNA学会年会, Jul. 2007, Japanese, 日本RNA学会, 名古屋, Domestic conference
    Invited oral presentation

  • ゼブラフィッシュ生殖細胞形成過程に働くRNAプログラム
    INOUE Kunio
    2007年度国立遺伝学研究所研究会「生殖細胞と生殖腺形成の普遍性と多様性」, Jul. 2007, Japanese, 国立遺伝学研究所, 三島, Domestic conference
    Invited oral presentation

  • Differential regulation of germline mRNAs in soma and germ cells by zebrafish miR-430
    Yuichiro Mishima, Antonio J. Giraldez, Yasuaki Takeda, Toshinobu Fujiwara, Hiroshi Sakamoto, Alexander F. Schier, Kunio Inoue
    The 5th European Zebrafish Genetics and Development Meeting, Jul. 2007, English, Amesterdam, International conference
    Poster presentation

  • Regulation of tissue specific splicing by RNA binding protein Fox-1
    Kazuhiro Fukumura, Ayako Kato, Yui Jin, Toshinobu Fujiwara, Hiroshi Sakamoto, Kunio Inoue
    第40回発生生物学会・第59回日本細胞生物学会, May 2007, English, 日本発生生物学会, 福岡, Domestic conference
    Poster presentation

  • Regulation of PGC-specific gene TDRD7 by zebrafish miR-430
    Yasuaki Takeda, Yuichiro Mishima, Hiroshi Sakamoto, Kunio Inoue
    第40回発生生物学会・第59回日本細胞生物学会, May 2007, English, 日本発生生物学会, 福岡, Domestic conference
    Poster presentation

  • Differential regulation of germline mRNAs in soma and germ cells by zebrafish miR-430
    INOUE Kunio
    第40回発生生物学会・第59回日本細胞生物学会, May 2007, English, 日本発生生物学会, 福岡, Domestic conference
    Invited oral presentation

  • Regulation of tissue specific splicing by RNA binding protein Fox-1
    Kazuhiro Fukumura, Ayako Kato, Yui Jin, Toshinobu Fujiwara, Hiroshi Sakamoto, Kunio Inoue
    日本分子生物学会第7回春季シンポジウム, Apr. 2007, English, 日本分子生物学会, 淡路, Domestic conference
    Poster presentation

  • Neuronal RNA binding protein HuD interact with protein kinase B
    FUKAO Akira, FUJIWARA Toshinobu, MATSUZAKI Hidenori, INOUE Kunio, KIKKAWA Ushio, SAKAMOTO Hiroshi
    The 7th MBSJ Spring Symposium, Apr. 2007, English, 日本分子生物学会, 神戸市, International conference
    Poster presentation

  • Mechanism of pre-ribosomal RNA processing in Caenorhabditis elegans
    SASANO Yumi, HOKII Yusuke, USHIDA Chisato, INOUE Kunio, SAKAMOTO Hiroshi, FUJIWARA Toshinobu
    The 7th MBSJ Spring Symposium, Apr. 2007, English, 日本分子生物学会, 神戸市, International conference
    Poster presentation

  • miRNA制御システムによる生殖細胞・体細胞分化確立機構
    井上 邦夫
    日本分子生物学会2006フォーラム, Dec. 2006, Japanese, 名古屋, Domestic conference
    Invited oral presentation

  • RNAプログラムによる生殖細胞形成機構
    井上 邦夫
    第17回フォーラム・イン・ドージン, Nov. 2006, Japanese, 熊本, Domestic conference
    Invited oral presentation

  • Fox蛋白質によるスプライシング制御機構
    井上 邦夫
    第2回 神戸スプライシングカンファレンス, Nov. 2006, Japanese, 神戸, Domestic conference
    Invited oral presentation

  • Regulation of tissue-specific splicing by Fox protein
    井上 邦夫
    20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, Jun. 2006, English, 京都, Domestic conference
    Invited oral presentation

  • リボソ-ムの異常と疾患 ～ゼブラフィッシュを用いた解析.
    UECHI Tamayo, NAKASHIMA Yukari, MISHIMA Yuichiro, INOUE Kunio, KENMOCHI Naoya
    第28回日本分子生物学会年会, Dec. 2005, Japanese, 福岡, Domestic conference
    Oral presentation

  • Control of maternal mRNA localization required for germ cell formation in zebrafish.
    KOSAKA Kyoko, UEDA Yasunori, MISHIMA Yuichiro, KAWAKAMI Koichi, SAKAMOTO Hiroshi, INOUE Kunio
    COE日台国際シンポジウム, Nov. 2005, Japanese, 神戸大学, Domestic conference
    Oral presentation

  • Zebrafish nanos1 3'UTR represses translation in somatic cells by accelerating deadenylation.
    MISHIMA Yuichiro, FUJIWARA Toshinobu, SKAMOTO Hiroshi, INOUE Kunio
    COE日台国際シンポジウム, Oct. 2005, Japanese, 神戸大学, Domestic conference
    Oral presentation

  • Zebrafish nanos1 3'UTR represses translation in somatic cells by accelerating deadenylation.
    MISHIMA Yuichiro, SAKAMOTO Hiroshi, INOUE Kunio
    第十一回小型魚類研究集会, Sep. 2005, Japanese, 岡崎, Domestic conference
    Oral presentation

  • Evidence for the involvement of neuron-specific Hu proteins in the translational regulation.
    TAKEUCHI Setsuko, WADA, FUJIWARA Toshinobu, INOUE Kunio, SAKAMOTO Hiroshi
    EMBO Conference on Protein Synthesis and Translational Control, Sep. 2005, English, ドイツ, International conference
    Oral presentation

  • Bruno-like protein is localized to germ plasm during early cleavage stages in zebrafish.
    HASIMOTO Y, SUZUKI H, KAGEYAMA Y, YASUDA K, INOUE Kunio
    15th International Society of Developmental Biologists Congress, Sep. 2005, English, オ-ストラリア, International conference
    Oral presentation

  • ゼブラフィッシュを用いたリボソームタンパク質の機能解析.
    UECHI Tamayo, NAKASHIMA Yukari, MISHIMA Yuichiro, INOUE Kunio, KENMOCHI Naoya
    第7回RNAミ-ティング（日本RNA学会年会）, Aug. 2005, Japanese, 弘前大学, Domestic conference
    Oral presentation

  • Regulation of alternative splicing by Fox-1 via binding to GCAUG sequence upstream of alternative exon.
    FUKUMURA Kazuhiro, JIN Yui, KATO Atako, FUJIWARA Toshinobu, SAKAMOTO Hiroshi, INOUE Kunio
    Cold Spring Harbor Laboratory Meeting EUKARYOTIC mRNA PROCESSING, Aug. 2005, English, アメリカ, International conference
    Oral presentation

  • Control of nanos1 mRNA stability during PGC formation in zebrafish.
    MISHIMA Yuichiro, FUJIWARA Toshinobu, SAKAMOTO Hiroshi, INOUE Kunio
    4th European Zebrafish genetics and Development Meeting, Jul. 2005, English, ドイツ, International conference
    Oral presentation

  • ゼブラフィッシュ生殖細胞形成過程にはたらく母性mRNA局在化機構の解析
    KOSAKA Kyoko, UEDA Yasunori, MISHIMA Yuichiro, KAWAKAMI Koichi, SAKAMOTO Hiroshi, INOUE Kunio
    日本発生生物学会第38回大会, Jun. 2005, Japanese, 仙台, Domestic conference
    Oral presentation

  • MRG-1, A C. ELEGANS CHROMODOMAIN PROTEIN IS ESSENTIAL FOR PROPER GENE EXPRESSION IN THE PRIMORDIAL GERM CELLS.
    TAKASAKI Teruaki, HABARA Yasuaki, NISHIWAKI Kiyoji, FUJIWARA Toshinobu, NAKAYAMA Jun-ichi, INOUE Kunio, SAKAMOTO Hiroshi
    15th Biennial International C.elegans conference, Jun. 2005, English, アメリカ, International conference
    Oral presentation

  • Control of maternal mRNA localization required for germ cell formation in zebrafish.
    KOSAKA Kyoko, UEDA Yasunori, MISHIMA Yuichiro, KAWAKAMI Koichi, SAKAMOTO Hiroshi, INOUE Kunio
    FASEB Summer Research Conferences「Intracellular RNA Sorting, Transport & Localization」, Jun. 2005, English, アメリカ, International conference
    Oral presentation

  • ゼブラフィッシュ生殖細胞形成機構の分子発生生物学的解析.
    INOUE Kunio
    基礎生物学研究所研究会「小型魚類のさらなる発展を求めて」, May 2005, Japanese, 岡崎, Domestic conference
    Oral presentation

  • RNA結合タンパク質Fox-1による選択的スプライシング制御機構の解析.
    FUKUMURA Kazuhiro, FUJIWARA Tochinobu, SAKAMOTO Hiroshi, INOUE Kunio
    特定領域研究「RNA情報網」第3回サテライトミーティング『RNA情報発現系の仕組みと制御』, May 2005, Japanese, 三重県, Domestic conference
    Oral presentation

  • Regulation of 18S ribosomal RNA processing in Caenorhabditis elegans.
    SASANO Yumi, FUJIWARA Toshinobu, INOUE Kunio, SAKAMOTO Hiroshi
    The Tenth Annual Meeting of the RNA society, May 2005, English, カナダ, International conference
    Oral presentation

  • ゼブラフィッシュ生殖細胞形成過程に働くRNA－蛋白質複合体
    井上 邦夫
    日本分子生物学会第27回年会, Dec. 2004, Japanese, 日本分子生物学会, 神戸ポートアイランド, Domestic conference
    Others

  • ゼブラフィッシュにおける母性mRNA局在化機構の解析
    小坂 恭子, 三嶋 雄一郎, 川上 浩一, 坂本 博, 井上 邦夫
    第6回RNAミーティング, Aug. 2004, Japanese, 未記入, 熊本テルサ, Domestic conference
    Oral presentation

  • ゼブラフィッシュを用いたDaz遺伝子の機能解析
    三嶋 雄一郎, 前川 真吾, 坂本 博, 井上 邦夫
    日本発生生物学会第37回大会, Jun. 2004, Japanese, 日本発生生物学会, 名古屋国際会議場, Domestic conference
    Poster presentation

  • ゼブラフィッシュにおける母性mRNA局在化機構の解析
    小坂 恭子, 三嶋 雄一郎, 川上 浩一, 坂本 博, 井上 邦夫
    日本発生生物学会第37回大会, Jun. 2004, Japanese, 日本発生生物学会, 名古屋国際会議場, Domestic conference
    Oral presentation

  • ゼブラフィッシュにおける母性mRNA局在化機構の解析
    小坂 恭子, 三嶋 雄一郎, 川上 浩一, 坂本 博, 井上 邦夫
    第37回日本発生生物学会, Jun. 2004, Japanese, 日本発生生物学会, 名古屋国際会議場, Domestic conference
    Poster presentation

  • ゼブラフィッシュにおけるリボソーム変異モデルの作製
    上地 珠代, 三嶋 雄一郎, 井上 邦夫, 剣持 直哉
    第6回日本RNA学会年会, Jun. 2004, Japanese, 日本RNA学会, 熊本テルサ, Domestic conference
    Poster presentation

  • ゼブラフィッシュ生殖細胞形成における母性供与因子群の役割
    橋本 祥子, 前川 真吾, 鈴木 仁, 安田 國雄, 井上 邦夫
    日本分子生物学会春季シンポジウム, May 2004, Japanese, 日本分子生物学会, 奈良県新公会堂, Domestic conference
    Others

  • ゼブラフィッシュ生殖細胞に存在するRNPの解析
    三嶋 雄一郎, 前川 真吾, 鈴木 仁, 橋本 祥子, 坂本 博, 井上 邦夫
    RNA研究若手の会2004「高次生命現象におけるRNA制御」, May 2004, Japanese, 未記入, 淡路夢舞台国際会議場, Domestic conference
    Others

  • ゼブラフィッシュにおける母性mRNA局在化機構の解析
    小坂 恭子, 三嶋 雄一郎, 川上 浩一, 坂本 博, 井上 邦夫
    RNA研究若手の会2004「高次生命現象におけるRNA制御」, May 2004, Japanese, 未記入, 淡路夢舞台国際会議場, Domestic conference
    Others

  • Functional analysis of zebrafish DAZL protein during germ cell formation.
    MISHIMA Y, SAKAMOTO Hiroshi, INOUE Kunio
    Conference on lipid messenger signaling , The first COE International Meeting., Jan. 2004, English, 未記入, Kobe, Domestic conference
    Oral presentation

  • 神経特異的Hu蛋白質の機能解析
    斎藤 都暁, 藤原 俊伸, 井上 邦夫, 坂本 博
    第26回日本分子生物学会, Dec. 2003, Japanese, 日本分子生物学会, 神戸ポートアイランド、神戸市, Domestic conference
    Oral presentation

  • ゼブラフィッシュにおけるリボソームタンパク質遺伝子の発現抑制
    上地 珠代, 橋本 祥子, 井上 邦夫, 剣持 直哉
    第26回日本分子生物学会, Dec. 2003, Japanese, 日本分子生物学会, 神戸ポートアイランド、神戸市, Domestic conference
    Poster presentation

  • ゼブラフィッシュPGC形成過程におけるzDazlの機能解析
    三嶋 雄一郎, 前川 真吾, 藤原 俊伸, 坂本 博, 井上 邦夫
    第26回日本分子生物学会, Dec. 2003, Japanese, 日本分子生物学会, 神戸ポートアイランド、神戸市, Domestic conference
    Poster presentation

  • Fox-1による選択的スプライシング制御機構の解析
    神 唯, 鈴木 仁, 前川 真吾, 遠藤 仁司, 安田 國雄, 坂本 博, 井上 邦夫
    第26回日本分子生物学会, Dec. 2003, Japanese, 日本分子生物学会, 神戸ポートアイランド、神戸市, Domestic conference
    Oral presentation

  • Ribosomal protein gene knockdown using the antisense oligos in zebrafish.
    上地 珠代, 橋本 祥子, 井上 邦夫, 剣持 直哉
    国際シンポジウム ”The New Frontier of RNA science”, Nov. 2003, English, 未記入, 国立京都国際会館, Domestic conference
    Poster presentation

  • Microubule association of a neuronal RNA-binding protein HuD through its binding to the light chain of MAP1B.
    斎藤 都暁, 藤原 俊伸, 笠島 克巳, 井上 邦夫, 坂本 博
    国際シンポジウム ”The New Frontier of RNA science”, Nov. 2003, English, 未記入, 国立京都国際会館, Domestic conference
    Oral presentation

  • Functional analysis of zDazl protein during PGC formation in zebrafish.
    三嶋 雄一郎, 前川 真吾, 藤原 俊伸, 坂本 博, 井上 邦夫
    国際シンポジウム ”The New Frontier of RNA science”, Nov. 2003, English, 未記入, 国立京都国際会館, Domestic conference
    Poster presentation

  • Fox-1 functions as both positive and negative regulator of alternative splicing in vertebrates.
    神 唯, 安田 國雄, 坂本 博, 井上 邦夫
    国際シンポジウム ”The New Frontier of RNA science”, Nov. 2003, English, 未記入, 国立京都国際会館, Domestic conference
    Poster presentation

  • ゼブラフィッシュ生殖細胞形成過程に働くRNA情報発現制御機能
    三嶋 雄一郎, 前川 真吾, 坂本 博, 井上 邦夫
    第9回小型魚類研究集会, Sep. 2003, Japanese, 未記入, 理化学研究所、和光市, Domestic conference
    Oral presentation

  • ゼブラフィッシュにおける母性ｍRNA局在化機構の解明
    小坂 恭子, 橋本 祥子, 前川 真吾, 安田 國雄, 坂本 博, 井上 邦夫
    第9回小型魚類研究集会, Sep. 2003, Japanese, 未記入, 理化学研究所、和光市, Domestic conference
    Poster presentation

  • Fox-1 functions as both positive and negative regulator of alternative splicing in vertebrates.
    神 唯, 安田 國雄, 坂本 博, 井上 邦夫
    Eukaryotic mRNA processing meeting, Aug. 2003, English, 未記入, CSHL, ニューヨーク, International conference
    Poster presentation

  • Requirement of localized Maternal Factors for Zebrafish Germ Cell Formation
    橋本 祥子, 前川 真吾, 安田 國雄, 井上 邦夫
    Society for Developmental Biology 62nd Annual Meeting Jointly with the International Society of Developmental Biology, Jul. 2003, English, Society for Developmental Biology, Marriott Copley Place, ボストン, International conference
    Poster presentation

  • Fox-1による選択的スプライシング制御機能の解析
    神 唯, 坂本 博, 井上 邦夫
    RNA研究者若手の会2003「真核生物におけるmRNA情報発現」, Jul. 2003, Japanese, RNA研究者若手の会, ウェルサンピア滋賀, Domestic conference
    Others

  • 異時的に移植されたゼブラフィッシュ始原生殖細胞（PGCs）の移動および分化
    長井 輝美, 斎藤 大輝, 吉川 智仁, 大谷 哲, 前川 真吾, 井上 邦夫, 荒井 克俊, 山羽 悦郎
    日本水産学会, Apr. 2003, Japanese, 日本水産学会, 東京水産大学, Domestic conference
    Oral presentation

■ Affiliated Academic Society
  • 日本RNA学会

  • 日本発生生物学会

  • 日本分子生物学会

■ Research Themes
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