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FUJIMOTO RyoGraduate School of Agricultural Science / Department of Bioresource ScienceAssociate Professor
Researcher basic information
■ Research news- 20 Aug. 2019, The mechanism that controls Chinese cabbage flowering
- 27 Oct. 2016, DNA methylation affects superiority of hybrid plants -Discovery could be used in efficient breeding of high-yield vegetables-
- Environmental science/Agricultural science / Plant genetics and breeding
- Environmental science/Agricultural science / Horticulture
Research activity information
■ Award- Jan. 2018 神戸大学, 平成29年度優秀若手研究賞, 雑種強勢研究Others
- Oct. 2017 神戸大学農学部同窓会六篠会, 六篠論文賞, PNAS誌に掲載された論文Others
- Mar. 2017 日本育種学会, 平成28年度日本育種学会奨励賞, アブラナ科植物におけるゲノム多様性および雑種強勢に関する研究Japan society
- International Society for Horticultural Science (ISHS), Sep. 2024, Acta Horticulturae, (1404) (1404), 1051 - 1058, EnglishScientific journal
- Corresponding, International Society for Horticultural Science (ISHS), Sep. 2024, Acta Horticulturae, (1404) (1404), 469 - 476, EnglishScientific journal
- International Society for Horticultural Science (ISHS), Sep. 2024, Acta Horticulturae, (1404) (1404), 323 - 330Scientific journal
- Abstract Premise With the global atmospheric CO2 concentration on the rise, developing crops that can thrive in elevated CO2 has become paramount. We investigated the potential of hybridization as a strategy for creating crops with improved growth in predicted elevated atmospheric CO2. Methods We grew parent accessions and their F1 hybrids of Arabidopsis thaliana in ambient and elevated atmospheric CO2 and analyzed numerous growth traits to assess their productivity and underlying mechanisms. Results The heterotic increase in total dry mass, relative growth rate and leaf net assimilation rate was significantly greater in elevated CO2 than in ambient CO2. The CO2 response of net assimilation rate was positively correlated with the CO2 response of leaf nitrogen productivity and with that of leaf traits such as leaf size and thickness, suggesting that hybridization‐induced changes in leaf traits greatly affected the improved performance in elevated CO2. Conclusions Vegetative growth of hybrids seems to be enhanced in elevated CO2 due to improved photosynthetic nitrogen‐use efficiency compared with parents. The results suggest that hybrid crops should be well‐suited for future conditions, but hybrid weeds may also be more competitive.Wiley, Apr. 2024, American Journal of Botany, 111(4) (4), e16317 - e16317, English[Refereed]Scientific journal
- Maximum Academic Press, 2024, Vegetable Research, 4(1) (1), e031, English[Refereed]Scientific journal
- UNLABELLED: Brassica rapa L., which includes Chinese cabbage, turnip, and pak choi, has more complex flowering time regulation than does Arabidopsis thaliana due to the presence of multiple paralogous flowering time genes. FLOWERING LOCUS C (FLC) is one of the key genes regulating the flowering time, and B. rapa has four FLC paralogs. BrFLC5 on the reference genome is deemed a pseudogene because of a mutation (from G to A) in the splice site of the third intron, but there are some accessions with a G nucleotide in the splice site. In this study, we genotyped 310 B. rapa accessions and found that 19 had homozygous and 81 had heterozygous putative functional BrFLC5 alleles. Accessions of turnip showed the highest proportion with a functional BrFLC5 allele. BrFLC5 acts as a floral repressor when overexpressed in A. thaliana. The BrFLC5 expression level varied in pre-vernalized plants, and this transcriptional variation was not associated with the G/A polymorphism in the third intron. Three accessions having a higher BrFLC5 expression in pre-vernalized plants had a 584-bp insertion in the promoter region. Many regions homologous to this 584-bp sequence are present in the B. rapa genome, and this 584-bp inserted region has tandem duplications of an AT-rich sequence in its central region. The possibility that a high expression of a functional BrFLC5 could contribute to producing premature bolting-resistant lines in B. rapa vegetables is discussed. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11032-023-01405-0.Corresponding, Aug. 2023, Molecular breeding : new strategies in plant improvement, 43(8) (8), 58 - 58, English, International magazine[Refereed]Scientific journal
- Corresponding, Frontiers Media SA, Jul. 2023, Frontiers in Plant Science, 14, 1239713, English[Refereed][Invited]Scientific journal
- Elsevier BV, Jun. 2023, Scientia Horticulturae, 316, 112002 - 112002, English[Refereed]Scientific journal
- Albugo candida causing white rust disease decreases the yield of Brassica rapa vegetables greatly. Resistant and susceptible cultivars in B. rapa vegetables have different immune responses against A. candida inoculation, however, the mechanism of how host plants respond to A. candida is still unknown. Using RNA-sequencing, we identified differentially expressed genes (DEGs) between A. candida inoculated [48 and 72 h after inoculation (HAI)] and non-inoculated samples in resistant and susceptible cultivars of komatsuna (B. rapa var. perviridis). Functional DEGs differed between the resistant and susceptible cultivars in A. candida inoculated samples. Salicylic acid (SA) responsive genes tended to be changed in their expression levels by A. candida inoculation in both resistant and susceptible cultivars, but different genes were identified in the two cultivars. SA-dependent systemic acquired resistance (SAR) involving genes were upregulated following A. candida inoculation in the resistant cultivar. Particular genes categorized as SAR that changed expression levels overlapped between A. candida and Fusarium oxysporum f. sp. conglutinans inoculated samples in resistant cultivar, suggesting a role for SAR in defense response to both pathogens particularly in the effector-triggered immunity downstream pathway. These findings will be useful for understanding white rust resistance mechanisms in B. rapa.Corresponding, May 2023, Scientific Reports, 13(1) (1), 8599 - 8599, English, International magazine[Refereed]Scientific journal
- Heterosis is an important phenomenon for high-yield crop breeding and is utilized for breeding F1 varieties in horticultural crops. However, its molecular mechanism has not been elucidated, and compared to cereals, heterosis is less explored at the molecular level in horticultural crops. In this review, we compiled the new genetic and epigenetic studies on heterosis in horticultural crops. Because of the difficulty of predicting the level of heterosis from the parental genetic distance, molecular approaches are being used to study its molecular basis in horticultural crops. Transcriptome analyses in vegetables have identified photosynthesis-related genes as important in heterosis. Analysis of noncoding RNAs has suggested their involvement in regulating the heterosis of vegetative and fruit tissues. Quantitative trait locus (QTL) analysis has revealed the association of heterozygosity of a specific locus or multiple loci with heterosis of vegetative and fruit tissues. A higher level of DNA methylation was noted in the heterotic F1 of Brassica rapa leafy vegetables, while the roles of other epigenetic modifications such as histone marks have not been explored.Corresponding, MDPI AG, Mar. 2023, Horticulturae, 9(3) (3), 366 - 366, English[Refereed][Invited]Scientific journal
- Corresponding, International Society for Horticultural Science (ISHS), Mar. 2023, Acta Horticulturae, (1362) (1362), 65 - 70, English[Refereed]Scientific journal
- Corresponding, International Society for Horticultural Science (ISHS), Mar. 2023, Acta Horticulturae, (1362) (1362), 107 - 112Scientific journal
- International Society for Horticultural Science (ISHS), Mar. 2023, Acta Horticulturae, (1362) (1362), 305 - 312Scientific journal
- Cabbage (Brassica oleracea var. capitata) Fusarium wilt (CFW) is a disease that poses a critical threat to global cabbage production. Screening for resistant resources in order to support the breeding of resistant cultivars is the most reliable approach to control this disease. CFW is caused by Fusarium oxysporum f. sp. conglutinans (Foc), which consists of two physiological races (race 1 and 2). While many studies have focused on resistance screening, gene mining, and inheritance-based research associated with resistance to Foc race 1, there have been few studies specifically analyzing resistance to Foc race 2, which is a potential threat that can overcome type A resistance. Here, 166 cabbage resources collected from around the world were evaluated for the resistance to both Foc races, with 46.99% and 38.55% of these cabbage lines being resistant to Foc race 1 and race 2, respectively, whereas 33.74% and 48.80% were susceptible to these two respective races. Of these 166 analyzed cabbage lines, 114 (68.67%) were found to be more susceptible to race 2 than to race 1, and 28 of them were resistant to race 1 while susceptible to race 2, underscoring the highly aggressive nature of Foc race 2. To analyze the inheritance of Foc race 2 resistance, segregated populations derived from the resistant parental line 'Badger Inbred 16' and the susceptible one '01-20' were analyzed with a major gene plus polygene mixed genetic model. The results of this analysis revealed Foc race 2-specific resistance to be under the control of two pairs of additive-dominant-epistatic major genes plus multiple additive-dominant-epistatic genes (model E). The heritability of these major genes in the BC1P1, BC1P2, and F2 generations were 32.14%, 72.80%, and 70.64%, respectively. In summary, these results may aid in future gene mining and breeding of novel CFW-resistant cabbage cultivars.Corresponding, Sep. 2022, Genes, 13(9) (9), English, International magazine[Refereed]Scientific journal
- If food and feed production are to keep up with world demand in the face of climate change, continued progress in understanding and utilizing both genetic and epigenetic sources of crop variation is necessary. Progress in plant breeding has traditionally been thought to be due to selection for spontaneous DNA sequence mutations that impart desirable phenotypes. These spontaneous mutations can expand phenotypic diversity, from which breeders can select agronomically useful traits. However, it has become clear that phenotypic diversity can be generated even when the genome sequence is unaltered. Epigenetic gene regulation is a mechanism by which genome expression is regulated without altering the DNA sequence. With the development of high throughput DNA sequencers, it has become possible to analyze the epigenetic state of the whole genome, which is termed the epigenome. These techniques enable us to identify spontaneous epigenetic mutations (epimutations) with high throughput and identify the epimutations that lead to increased phenotypic diversity. These epimutations can create new phenotypes and the causative epimutations can be inherited over generations. There is evidence of selected agronomic traits being conditioned by heritable epimutations, and breeders may have historically selected for epiallele-conditioned agronomic traits. These results imply that not only DNA sequence diversity, but the diversity of epigenetic states can contribute to increased phenotypic diversity. However, since the modes of induction and transmission of epialleles and their stability differ from that of genetic alleles, the importance of inheritance as classically defined also differs. For example, there may be a difference between the types of epigenetic inheritance important to crop breeding and crop production. The former may depend more on longer-term inheritance whereas the latter may simply take advantage of shorter-term phenomena. With the advances in our understanding of epigenetics, epigenetics may bring new perspectives for crop improvement, such as the use of epigenetic variation or epigenome editing in breeding. In this review, we will introduce the role of epigenetic variation in plant breeding, largely focusing on DNA methylation, and conclude by asking to what extent new knowledge of epigenetics in crop breeding has led to documented cases of its successful use.Lead, Sep. 2022, Frontiers in plant science, 13, 958350 - 958350, English, International magazine[Refereed]Scientific journal
- Tuberous stem of kohlrabi is an important agronomic trait, however, the molecular basis of tuberization is poorly understood. To elucidate the tuberization mechanism, we conducted a comparative transcriptomic analysis between kohlrabi and broccoli at 10 and 20 days after germination (DAG) as tuberous stem initiated between these time points. A total of 5580 and 2866 differentially expressed transcripts (DETs) were identified between genotypes (kohlrabi vs broccoli) and growth stages (10 DAG vs 20 DAG), respectively, and most of the DETs were down-regulated in kohlrabi. Gene ontology (GO) and KEGG pathway enrichment analyses showed that the DETs between genotypes are involved in cell wall loosening and expansion, cell cycle and division, carbohydrate metabolism, hormone transport, hormone signal transduction, and in several transcription factors. The DETs identified in those categories may directly/indirectly relate to the initiation and development of tuberous stem in kohlrabi. In addition, the expression pattern of the hormone synthesis related DETs coincided with the endogenous IAA, IAAsp, GA, ABA and tZ profiles in kohlrabi and broccoli seedlings, that were revealed in our previous phytohormone analysis. This is the first report on comparative transcriptome analysis for tuberous stem formation in kohlrabi at early growth periods. The resulting data could provide significant insights into the molecular mechanism underlying tuberous stem development in kohlrabi as well as in other tuberous organ forming crops. This article is protected by copyright. All rights reserved.Aug. 2022, Physiologia plantarum, e13770, English, International magazine[Refereed]Scientific journal
- DNA methylation is an epigenetic mark associated with several mechanisms in plants including immunity mechanisms. However, little is known about the regulatory role of DNA methylation in the resistance response of Brassica species against fungal diseases. White rust, caused by the fungus Albugo candida, is one of the most widespread and destructive diseases of all the cultivated Brassica species, particularly Brassica rapa L. and Brassica juncea (L.) Czern and Coss. Here, we investigate whole-genome DNA methylation modifications of B. rapa subsp. perviridis in response to white rust. As a result, 233 and 275 differentially methylated regions (DMRs) in the susceptible cultivar “Misugi” and the resistant cultivar “Nanane” were identified, respectively. In both cultivars, more than half of the DMRs were associated with genes (DMR-genes). Gene expression analysis showed that 13 of these genes were also differentially expressed between control and infected samples. Gene ontology enrichment analysis of DMR genes revealed their involvement in various biological processes including defense mechanisms. DMRs were unevenly distributed around genes in susceptible and resistant cultivars. In “Misugi,” DMRs tended to be located within genes, while in “Nanane,” DMRs tended to be located up and downstream of the genes. However, CG DMRs were predominantly located within genes in both cultivars. Transposable elements also showed association with all three sequence contexts of DMRs but predominantly with CHG and CHH DMRs in both cultivars. Our findings indicate the occurrence of DNA methylation modifications in B. rapa in response to white rust infection and suggest a potential regulatory role of DNA methylation modification in defense mechanisms which could be exploited to improve disease resistance.Corresponding, Frontiers Media SA, Jun. 2022, Frontiers in Plant Science, 13, English[Refereed]Scientific journal
- Long noncoding RNAs (lncRNAs) play important roles in abiotic and biotic stress responses; however, studies on the mechanism of regulation of lncRNA expression are limited in plants. The present study examined the relationship between lncRNA expression level and two active histone modifications (H3K4me3 and H3K36me3) in Brassica rapa. Both histone marks were enriched in the chromatin regions encoding lncRNAs, especially around the transcription start site. The transcription level of long intergenic noncoding RNAs was positively associated with the level of H3K4me3 and H3K36me3, while this association was not observed in natural antisense RNAs (NATs) and intronic noncoding RNAs. As coordinate expression of mRNAs and paired NATs under biotic stress treatment has been identified, the transcriptional relationship between mRNAs and their paired NATs following Fusarium oxysporum f. sp. conglutinans (Foc) inoculation was examined. A positive association of expression levels between mRNAs and their paired NATs following Foc inoculation was observed. This association held for several defense-response-related genes and their NAT pairs. These results suggest that coordinate expression of mRNAs and paired NATs plays a role in the defense response against Foc.Corresponding, MDPI AG, Dec. 2021, Horticulturae, 8(1) (1), 17 - 17, English[Refereed]Scientific journal
- Covalent modifications of histone proteins act as epigenetic regulators of gene expression. We report the distribution of two active histone marks (H3K4me3 and H3K36me3) in 14-day leaves in two lines ofCorresponding, Frontiers Media SA, Jun. 2021, Frontiers in Plant Science, 12, 659634 - 659634, English, International magazine
Brassica rapa L. by chromatin immunoprecipitation sequencing. Both lines were enriched with H3K4me3 and H3K36me3 marks at the transcription start site, and the transcription level of a gene was associated with the level of H3K4me3 and H3K36me3. H3K4me3- and H3K36me3-marked genes showed low tissue-specific gene expression, and genes with both H3K4me3 and H3K36me3 had a high level of expression and were constitutively expressed. Bivalent active and repressive histone modifications such as H3K4me3 and H3K27me3 marks or antagonistic coexistence of H3K36me3 and H3K27me3 marks were observed in some genes. Expression may be susceptible to changes by abiotic and biotic stresses in genes having both H3K4me3 and H3K27me3 marks. We showed that the presence of H3K36me3 marks was associated with different gene expression levels or tissue specificity between paralogous paired genes, suggesting that H3K36me3 might be involved in subfunctionalization of the subgenomes.[Refereed]Scientific journal - In vegetables of Brassica rapa L., Fusarium oxysporum f. sp. rapae (For) or F. oxysporum f. sp. conglutinans (Foc) cause Fusarium yellows. A resistance gene against Foc (FocBr1) has been identified, and deletion of this gene results in susceptibility (focbr1-1). In contrast, a resistance gene against For has not been identified. Inoculation tests showed that lines resistant to Foc were also resistant to For, and lines susceptible to Foc were susceptible to For. However, prediction of disease resistance by a dominant DNA marker on FocBr1 (Bra012688m) was not associated with disease resistance of For in some komatsuna lines using an inoculation test. QTL-seq using four F2 populations derived from For susceptible and resistant lines showed one causative locus on chromosome A03, which covers FocBr1. Comparison of the amino acid sequence of FocBr1 between susceptible and resistant alleles (FocBr1 and FocBo1) showed that six amino acid differences were specific to susceptible lines. The presence and absence of FocBr1 is consistent with For resistance in F2 populations. These results indicate that FocBr1 is essential for For resistance, and changed amino acid sequences result in susceptibility to For. This susceptible allele is termed focbr1-2, and a new DNA marker (focbr1-2m) for detection of the focbr1-2 allele was developed.Corresponding, MDPI AG, May 2021, Plants, 10(6) (6), 1082 - 1082, English, International magazine[Refereed]Scientific journal
- KEY MESSAGE: Fusarium yellows resistant and susceptible lines in Brassica rapa showed different salicylic acid responses; the resistant line showed a similar response to previous reports, but the susceptible line differed. Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans (Foc) is an important disease. Previous studies showed that genes related to salicylic acid (SA) response were more highly induced following Foc infection in Brassica rapa Fusarium yellows resistant lines than susceptible lines. However, SA-induced genes have not been identified at the whole genome level and it was unclear whether they were up-regulated by Foc inoculation. Transcriptome analysis with and without SA treatment in the B. rapa Fusarium yellows susceptible line 'Misugi' and the resistant line 'Nanane' was performed to obtain insights into the relationship between SA sensitivity/response and Fusarium yellows resistance. 'Nanane's up-regulated genes were related to SA response and down-regulated genes were related to jasmonic acid (JA) or ethylene (ET) response, but differentially expressed genes in 'Misugi' were not. This result suggests that Fusarium yellows resistant and susceptible lines have a different SA response and that an antagonistic transcription between SA and JA/ET responses was found only in a Fusarium yellows resistant line. SA-responsive genes were induced by Foc inoculation in Fusarium yellows resistant (RJKB-T23) and susceptible lines (RJKB-T24). By contrast, 39 SA-induced genes specific to RJKB-T23 might function in the defense response to Foc. In this study, SA-induced genes were identified at the whole genome level, and the possibility, the defense response to Foc observed in a resistant line could be mediated by SA-induced genes, is suggested. These results will be useful for future research concerning the SA importance in Foc or other diseases resistance in B. rapa.Corresponding, Apr. 2021, Plant cell reports, 40(4) (4), 605 - 619, English, International magazine[Refereed]Scientific journal
- Long noncoding RNAs (lncRNAs) are RNA fragments that generally do not code for a protein but are involved in epigenetic gene regulation. In this study, lncRNAs of Brassica rapa were classified into long intergenic noncoding RNAs, natural antisense RNAs, and intronic noncoding RNAs and their expression analyzed in relation to genome-wide 24-nt small interfering RNAs (siRNAs), DNA methylation, and histone H3 lysine 27 trimethylation marks (H3K27me3). More than 65% of the lncRNAs analyzed consisted of one exon, and more than 55% overlapped with inverted repeat regions (IRRs). Overlap of lncRNAs with IRRs or genomic regions encoding for 24-nt siRNAs resulted in increased DNA methylation levels when both were present. LncRNA did not overlap greatly with H3K27me3 marks, but the expression level of intronic noncoding RNAs that did coincide with H3K27me3 marks was higher than without H3K27me3 marks. The Brassica genus comprises important vegetables and oil seed crops grown across the world. B. rapa is a diploid (AA genome) thought to be one of the ancestral species of both B. juncea (AABB genome) and B. napus (AACC) through genome merging (allotetrapolyploidization). Complex genome restructuring and epigenetic alterations are thought to be involved in these allotetrapolyploidization events. Comparison of lncRNAs between B. rapa and B. nigra, B. oleracea, B. juncea, and B. napus showed the highest conservation with B. oleracea. This study presents a comprehensive analysis of the epigenome structure of B. rapa at multi-epigenetic levels (siRNAs, DNA methylation, H3K27me3, and lncRNAs) and identified a suite of candidate lncRNAs that may be epigenetically regulated in the Brassica genus.2021, PloS one, 16(3) (3), e0242530, English, International magazine[Refereed]Scientific journal
- The genus Brassica contains important vegetable crops, which serve as a source of oil seed, condiments, and forages. However, their production is hampered by various diseases such as clubroot and Fusarium wilt, especially in Brassica vegetables. Soil-borne diseases are difficult to manage by traditional methods. Host resistance is an important tool for minimizing disease and many types of resistance (R) genes have been identified. More than 20 major clubroot (CR) disease-related loci have been identified in Brassica vegetables and several CR-resistant genes have been isolated by map-based cloning. Fusarium wilt resistant genes in Brassica vegetables have also been isolated. These isolated R genes encode the toll-interleukin-1 receptor/nucleotide-binding site/leucine-rice-repeat (TIR-NBS-LRR) protein. DNA markers that are linked with disease resistance allele have been successfully applied to improve disease resistance through marker-assisted selection (MAS). In this review, we focused on the recent status of identifying clubroot and Fusarium wilt R genes and the feasibility of using MAS for developing disease resistance cultivars in Brassica vegetables.Jun. 2020, Plants (Basel, Switzerland), 9(6) (6), English, International magazine[Refereed]Scientific journal
- Corresponding, Mar. 2020, Plants, 9(4) (4), 414Hasan Mehraj;Takahiro Kawanabe; Motoki Shimizu; Naomi Miyaji; Ayasha Akter; Elizabeth S. Dennis; Ryo Fujimoto[Refereed]Scientific journal
- The genus Brassica includes oil crops, vegetables, condiments, fodder crops, and ornamental plants. Brassica species underwent a whole genome triplication event after speciation between ancestral species of Brassica and closely related genera including Arabidopsis thaliana. Diploid species such as Brassica rapa and Brassica oleracea have three copies of genes orthologous to each A. thaliana gene, although deletion in one or two of the three homologs has occurred in some genes. The floral transition is one of the crucial events in a plant's life history, and time of flowering is an important agricultural trait. There is a variation in flowering time within species of the genus Brassica, and this variation is largely dependent on a difference in vernalization requirements. In Brassica, like in A. thaliana, the key gene of vernalization is FLOWERING LOCUS C (FLC). In Brassica species, the vernalization response including the repression of FLC expression by cold treatment and the enrichment of the repressive histone modification tri-methylated histone H3 lysine 27 (H3K27me3) at the FLC locus is similar to A. thaliana. B. rapa and B. oleracea each have four paralogs of FLC, and the allotetraploid species, Brassica napus, has nine paralogs. The increased number of paralogs makes the role of FLC in vernalization more complicated; in a single plant, paralogs vary in the expression level of FLC before and after vernalization. There is also variation in FLC expression levels between accessions. In this review, we focus on the regulatory circuits of the vernalization response of FLC expression in the genus Brassica.Corresponding, 2020, Frontiers in plant science, 11, 619417 - 619417, English, International magazine[Refereed]Scientific journal
- Last, Japanese Society for Horticultural Science, 2020, The Horticulture Journal, 89(3) (3), 268 - 277, English[Refereed]Scientific journal
- Oct. 2019, The Horticulture Journal, 88(4) (4), 462 - 470, EnglishComparison of Cold Responses for Orthologs of Cabbage Vernalization-related Genes[Refereed]Scientific journal
- Brassica rapa L. is an important vegetable and oilseed crop. We investigated the distribution of the histone mark tri-methylation of H3K27 (H3K27me3) in B. rapa and its role in the control of gene expression at two stages of development (2-day cotyledons and 14-day leaves) and among paralogs in the triplicated genome. H3K27me3 has a similar distribution in two inbred lines, while there was variation of H3K27me3 sites between tissues. Sites that are specific to 2-day cotyledons have increased transcriptional activity, and low levels of H3K27me3 in the gene body region. In 14-day leaves, levels of H3K27me3 were associated with decreased gene expression. In the triplicated genome, H3K27me3 is associated with paralogs that have tissue-specific expression. Even though B. rapa and Arabidopsis thaliana are not closely related within the Brassicaceae, there is conservation of H3K27me3-marked sites in the two species. Both B. rapa and A. thaliana require vernalization for floral initiation with FLC being the major controlling locus. In all four BrFLC paralogs, low-temperature treatment increases H3K27me3 at the proximal nucleation site reducing BrFLC expression. Following return to normal temperature growth conditions, H3K27me3 spreads along all four BrFLC paralogs providing stable repression of the gene.Oct. 2019, DNA research : an international journal for rapid publication of reports on genes and genomes, 26(5) (5), 433 - 443, English, International magazine[Refereed]Scientific journal
- There is a wide variation of flowering time among lines of Brassica rapa L. Most B. rapa leafy (Chinese cabbage etc.) or root (turnip) vegetables require prolonged cold exposure for flowering, known as vernalization. Premature bolting caused by low temperature leads to a reduction in the yield/quality of these B. rapa vegetables. Therefore, high bolting resistance is an important breeding trait, and understanding the molecular mechanism of vernalization is necessary to achieve this goal. In this study, we demonstrated that BrFRIb functions as an activator of BrFLC in B. rapa. We showed a positive correlation between the steady state expression levels of the sum of the BrFLC paralogs and the days to flowering after four weeks of cold treatment, suggesting that this is an indicator of the vernalization requirement. We indicate that BrFLCs are repressed by the accumulation of H3K27me3 and that the spreading of H3K27me3 promotes stable FLC repression. However, there was no clear relationship between the level of H3K27me3 in the BrFLC and the vernalization requirement. We also showed that if there was a high vernalization requirement, the rate of repression of BrFLC1 expression following prolonged cold treatments was lower.Sep. 2019, Scientific reports, 9(1) (1), 13843 - 13843, English, International magazine[Refereed]Scientific journal
- Jul. 2019, Breeding Science, 69, 308 - 315, EnglishAllele specific DNA marker for fusarium resistance gene FocBo1 in Brassica oleracea[Refereed]Scientific journal
- Brassica rapa L. is an important agricultural crop that requires a period of prolonged cold for flowering. This process is known as vernalization. Studies have shown that long noncoding RNAs (lncRNAs) play important roles in abiotic stress responses and several cold-responsive noncoding RNAs have been suggested to be involved in vernalization. We examined the transcriptome of the Chinese cabbage inbred line (B. rapa L. var. pekinensis) RJKB-T24, and identified 1,444 long intergenic noncoding RNAs (lincRNAs), 551 natural antisense transcripts (NATs), and 93 intronic noncoding RNAs (incRNAs); 549 of the 2,088 lncRNAs significantly altered their expression in response to four weeks of cold treatment. Most differentially expressed lncRNAs did not lead to a change of expression levels in mRNAs covering or near lncRNAs, suggesting that the transcriptional responses to four weeks of cold treatment in lncRNA and mRNA are independent. However, some differentially expressed mRNAs had NATs with expression altered in the same direction. These genes were categorized as having an abiotic stress response, suggesting that the paired-expression may play a role in the transcriptional response to vernalization or cold treatment. We also identified short-term cold treatment induced NATs in BrFLC and BrMAF genes, which are involved in vernalization. The lncRNAs we identified differed from those reported in Arabidopsis thaliana, suggesting the role of lncRNAs in vernalization differ between these two species.Jun. 2019, Scientific reports, 9(1) (1), 9302 - 9302, English, International magazine[Refereed]Scientific journal
- Japanese Society of Breeding, Jun. 2019, 育種学研究, 21(1) (1), 61 - 68, Japanese
- Dec. 2018, Plant & Cell Physiology, 59(12) (12), 2590 - 2601, EnglishGenome-Wide Analysis of Parent-of-Origin Allelic Expression in Endosperms of Brassicaceae Species, Brassica rapa.[Refereed]Scientific journal
- Epigenetic gene regulation is crucial to plant life and can involve dynamic interactions between various histone modifications, DNA methylation, and small RNAs. Detailed analysis of epigenome information is anticipated to reveal how the DNA sequence of the genome is translated into the plant's phenotype. The aim of this study was to map the DNA methylation state at the whole genome level and to clarify the relationship between DNA methylation and transcription, small RNA expression, and histone H3 lysine 9 di-methylation (H3K9me2) in Brassica rapa. We performed whole genome bisulfite sequencing, small RNA sequencing, and chromatin immunoprecipitation sequencing using H3K9me2 antibody in a Chinese cabbage inbred line, RJKB-T24, and examined the impact of epigenetic states on transcription. Cytosine methylation in DNA was analysed in different sequence contexts (CG, CHG, and CHH) (where H could be A, C, or T) and position (promoter, exon, intron, terminator, interspersed repeat regions), and the H3K9me2 and 24 nucleotide small interfering RNAs (24 nt-siRNA) were overlaid onto the B. rapa reference genome. The epigenome was compared with that of Arabidopsis thaliana and the relationship between the position of DNA methylation and gene expression, and the involvement of 24 nt siRNAs and H3K9me2 are discussed.Oct. 2018, DNA research : an international journal for rapid publication of reports on genes and genomes, 25(5) (5), 511 - 520, English, International magazine[Refereed]Scientific journal
- Sep. 2018, AusCanola 2018 Proceedings E-book, 127 - 131, EnglishHow Brassica oleracea FLOWERING LOCUS C 2 works in Brassica rapa genetic background[Invited]
- Sep. 2018, AusCanola 2018 Proceedings E-book, 71 - 75, EnglishCharacterization of FLOWERING LOCUS C genes in leafy Brassica rapa vegetables[Invited]
- Aug. 2018, Breeding Science, 68(3) (3), 316 - 325, EnglishThe production and characterization of a BoFLC2 introgressed Brassica rapa by repeated backcrossing to an F1[Refereed]Scientific journal
- Aug. 2018, Breeding Science, 68(3) (3), 375 - 380, EnglishGenome re-sequencing, SNP analysis, and genetic mapping of the parental lines of a commercial F1 hybrid cultivar of Chinese cabbage[Refereed]Scientific journal
- Heterosis or hybrid vigor is a phenomenon in which a hybrid progeny exhibits superior performance compared to their parental inbred lines. F1 hybrid seed production system is used for many crops and vegetables because of the high yield due to hybrid vigor. It has been over 100 years since the discovery of the hybrid vigor phenomenon however, the molecular mechanism of hybrid vigor is still a mystery. Understanding the mechanism of hybrid vigor will allow developing more efficient breeding systems and contribute to food security. At this time, hybrid vigor research has mainly used genetic approaches providing a number of findings in many plant species. High throughput sequencing will make genetic analysis more efficient, and large-scale quantitative trait analysis or genome wide association analysis has been performed in some plant species. However recent works have revealed that epigenetic regulations also contribute to hybrid vigor. In this chapter, we will discuss the most recent findings suggesting that epigenetic regulation may play important roles in hybrid vigor.Academic Press Inc., Jan. 2018, Advances in Botanical Research, 88, 247 - 275, EnglishScientific journal
- As climatic changes occur over the coming decades, our scientific understanding of plant responses to environmental cues will become an increasingly important consideration in the breeding of agricultural crops. This review provides a summary of the literature regarding vernalization research in Brassicaceae, covering both the historical origins of vernalization research and current understanding of the molecular mechanisms behind the regulatory pathways involved in vernalization and subsequent inflorescence. We discuss the evolutionarily conserved biology between the model organism Arabidopsis thaliana and the Brassica genus of crop cultivars and contrast the differences between the genera to illustrate the importance of Brassica-specific research into vernalization.CSIRO, 2018, Crop and Pasture Science, 69(1) (1), 30 - 39, English[Refereed]International conference proceedings
- DNA methylation is an epigenetic gene regulatory mechanism that plays an essential role in gene expression, transposon silencing, genome imprinting and plant development. We investigated the influence of DNA methylation on gene expression in Brassica rapa L., to understand whether epigenetic differences exist between inbred lines. Genome-wide DNA methylation was analysed by methylated DNA immunoprecipitation sequencing (MeDIP-seq) of 14-day-old first and second leaves from two inbred lines of Chinese cabbage, one susceptible and one resistant to fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans. MACS (model-based analysis for ChIP-seq) identified DNA methylation peaks in genic regions including 2kb upstream, exon, intron and 2kb downstream. More than 65% of genes showed similar patterns of DNA methylation in the genic regions in the two inbred lines. DNA methylation states of the two inbred lines were compared with their transcriptome. Genes having DNA methylation in the intron and in the 200bp upstream and downstream regions were associated with a lower expression level in both lines. A small number of genes showed a negative correlation between differences in DNA methylation levels and differences in transcriptional levels in the two inbred lines, suggesting that DNA methylation in these genes results in transcriptional suppression.CSIRO, 2018, Crop and Pasture Science, 69(1) (1), 107 - 120, English[Refereed]International conference proceedings
- Epigenetic regulation, covalent modification of DNA and changes in histone proteins are closely linked to plant development and stress response through flexibly altering the chromatin structure to regulate gene expression. In this review, we will illustrate the importance of epigenetic influences by discussing three agriculturally important traits of Brassicaceae. (1) Vernalization, an acceleration of flowering by prolonged cold exposure regulated through epigenetic silencing of a central floral repressor, FLOWERING LOCUS C. This is associated with cold-dependent repressive histone mark accumulation, which confers competency of consequence vegetative-to-reproductive phase transition. (2) Hybrid vigor, in which an F1 hybrid shows superior performance to the parental lines. Combination of distinct epigenomes with different DNA methylation states between parental lines is important for increase in growth rate in a hybrid progeny. This is independent of siRNA-directed DNA methylation but dependent on the chromatin remodeler DDM1. (3) Self-incompatibility, a reproductive mating system to prevent self-fertilization. This is controlled by the S-locus consisting of SP11 and SRK which are responsible for self/non-self recognition. Because self-incompatibility in Brassicaceae is sporophytically controlled, there are dominance relationships between S haplotypes in the stigma and pollen. The dominance relationships in the pollen rely on de novo DNA methylation at the promoter region of a recessive allele, which is triggered by siRNA production from a flanking region of a dominant allele.Springer Verlag, Jan. 2018, Plant Cell Reports, 37(1) (1), 87 - 101, English[Refereed]Scientific journal
- Heterosis or hybrid vigor is a phenomenon where hybrid progeny have superior performance compared to their parental inbred lines. This is important in the use of F1 hybrid cultivars in many crops and vegetables. However, the molecular mechanism of heterosis is not clearly understood. Gene interactions between the two genomes such as dominance, overdominance, and epistasis have been suggested to explain the increased biomass and yield. Genetic analyses of F1 hybrids in maize, rice, and canola have defined a large number of quantitative trait loci, which may contribute to heterosis. Recent molecular analyses of transcriptomes together with reference to the epigenome of the parents and hybrids have begun to uncover new facts about the generation of heterosis. These include the identification of gene expression changes in hybrids, which may be important for heterosis, the role of epigenetic processes in heterosis, and the development of stable high yielding lines.Japanese Society of Breeding, 2018, Breeding Science, 68(2) (2), 145 - 158, English[Refereed][Invited]Scientific journal
- Background: Breeding programs often rely on marker-assisted tests or variant calling of next generation sequence (NGS) data to identify regions of genomic introgression arising from the hybridization of two plant species. In this paper we present IntroMap, a bioinformatics pipeline for the screening of candidate plants through the application of signal processing techniques to NGS data, using alignment to a reference genome sequence (annotation is not required) that shares homology with the recurrent parental cultivar, and without the need for de novo assembly of the read data or variant calling. Results: We show the accurate identification of introgressed genomic regions using both in silico simulated genomes, and a hybridized cultivar data set using our pipeline. Additionally we show, through targeted marker-based assays, validation of the IntroMap predicted regions for the hybrid cultivar. Conclusions: This approach can be used to automate the screening of large populations, reducing the time and labor required, and can improve the accuracy of the detection of introgressed regions in comparison to a marker-based approach. In contrast to other approaches that generally rely upon a variant calling step, our method achieves accurate identification of introgressed regions without variant calling, relying solely upon alignment.BIOMED CENTRAL LTD, Dec. 2017, BMC GENETICS, 18, 101, English[Refereed]Scientific journal
- Resistant and susceptible lines in Brassica rapa have different immune responses against Fusarium oxysporum inoculation.Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans (Foc) is an important disease of Brassicaceae; however, the mechanism of how host plants respond to Foc is still unknown. By comparing with and without Foc inoculation in both resistant and susceptible lines of Chinese cabbage (Brassica rapa var. pekinensis), we identified differentially expressed genes (DEGs) between the bulked inoculated (6, 12, 24, and 72 h after inoculation (HAI)) and non-inoculated samples. Most of the DEGs were up-regulated by Foc inoculation. Quantitative real-time RT-PCR showed that most up-regulated genes increased their expression levels from 24 HAI. An independent transcriptome analysis at 24 and 72 HAI was performed in resistant and susceptible lines. GO analysis using up-regulated genes at 24 HAI indicated that Foc inoculation activated systemic acquired resistance (SAR) in resistant lines and tryptophan biosynthetic process and responses to chitin and ethylene in susceptible lines. By contrast, GO analysis using up-regulated genes at 72 HAI showed the overrepresentation of some categories for the defense response in susceptible lines but not in the resistant lines. We also compared DEGs between B. rapa and Arabidopsis thaliana after F. oxysporum inoculation at the same time point, and identified genes related to defense response that were up-regulated in the resistant lines of Chinese cabbage and A. thaliana. Particular genes that changed expression levels overlapped between the two species, suggesting that they are candidates for genes involved in the resistance mechanisms against F. oxysporum.SPRINGER, Dec. 2017, PLANT CELL REPORTS, 36(12) (12), 1841 - 1854, English[Refereed]Scientific journal
- Japanese Society of Breeding, Sep. 2017, 育種学研究, 19(3) (3), 116 - 123, Japanese[Invited]Scientific journal
- The tuberous stem of kohlrabi is an important quantitative trait, which affects its yield and quality. Genetic control of this trait has not yet been unveiled. To identify the QTLs controlling stem swelling of kohlrabi, a BC 1 population of 92 plants was developed from a cross of broccoli DH line GCP04 and kohlrabi var. Seine. A wide range of variation in tuberous stem diameter was observed among the mapping populations. We constructed a genetic map of nine linkage groups (LGs) with different types of markers, spanning a total length of 913.5 cM with an average marker distance of 7.55 cM. Four significant QTLs for radial enlargement of kohlrabi stem, namely, REnBo1, REnBo2, REnBo3, and REnBo4 were detected on C02, C03, C05, and C09, respectively, and accounted for the phenotypic variation of 59% for the stem diameter and 55% for the qualitative grading of tuberous stem in classes. Then, we confirmed the stability of identified QTLs using BC1S1 populations derived from the BC1 plants having heterozygous alleles at the target QTL and homozygous kohlrabi alleles at the remaining QTLs. REnBo1 and REnBo2 using 128 plants of BC(1)68S(1) and 94 plants of BC(1)43S(1), respectively, and REnBo3 and REnBo4 using 152 plants of BC(1)57S(1) were detected at the same positions as the respective QTLs of the BC1 population. Confirmation of QTLs in two successive generations indicates that the QTLs are persistent. The QTLs obtained in this study could be useful in marker-assisted selection of kohlrabi breeding, and to understand the genetic mechanisms of stem swelling and storage organ development in kohlrabi and other Brassica species.SPRINGER, Sep. 2017, MOLECULAR BREEDING, 37(9) (9), 109, English[Refereed]Scientific journal
- 日本育種学会, Apr. 2017, 育種学研究, 19(1) (1), 21 - 29, Japanese[Invited]
- Cabbage (Brassica oleracea L. var. capitata) is an important vegetable worldwide. Most Japanese commercial cultivars of cabbage use an F-1 hybrid seed production system. The purity of F-1 hybrid seeds is important and the assessment of purity based on DNA markers can be highly accurate. In addition, selection of agronomically important traits such as disease resistance based on DNA markers is useful for breeding of cabbage. The aim of this study is to demonstrate the effectiveness of DNA marker-assisted selection in cabbage. In this study we distinguished the parental S haplotypes in 35 F-1 hybrid cultivars by combining several linked DNA markers. Thirty-one highly polymorphic simple sequence repeats (SSR) markers were screened from 175 reported SSR markers, which are useful for assessment of the purity of F-1 hybrid seeds. We examined the relationship between the DNA marker based genotype and the phenotype by an inoculation test of clubroot disease. A co-dominant PCR-RFLP marker was developed for selection of Fusarium yellows resistance and the genotypes using this marker were consistent with inoculation test in all tested samples.SPRINGER, Jan. 2017, EUPHYTICA, 213(1) (1), 28, English[Refereed]Scientific journal
- Hybrid vigor or heterosis refers to the superior performance of F-1 hybrid plants over their parents. Heterosis is particularly important in the production systems of major crops. Recent studies have suggested that epigenetic regulation such as DNA methylation is involved in heterosis, but the molecular mechanism of heterosis is still unclear. To address the epigenetic contribution to heterosis in Arabidopsis thaliana, we used mutant genes that have roles in DNA methylation. Hybrids between C24 and Columbia-0 (Col) without RNA polymerase IV (Pol IV) or methyltransferase I (MET1) function did not reduce the level of biomass heterosis (as evaluated by rosette diameter). Hybrids with a mutation in decrease in dna methylation 1 (ddm1) showed a decreased heterosis level. Vegetative heterosis in the ddm1 mutant hybrid was reduced but not eliminated; a complete reduction could result if there was a change in methylation at all loci critical for generating the level of heterosis, whereas if only a proportion of the loci have methylation changes there may only be a partial reduction in heterosis.NATL ACAD SCIENCES, Oct. 2016, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 113(43) (43), E6704 - E6711, English[Refereed]Scientific journal
- Oct. 2016, Brassica 2016 Abstract Book, 172 - 177, EnglishGenome-wide analysis of DNA methylation in Chinese cabbageInternational conference proceedings
- Oct. 2016, Brassica 2016 Abstract Book, 185 - 190, EnglishIdentification of the Fusarium yellows resistance genes; its application for marker-assisted selection in Brassica rapa
- Oct. 2016, Brassica 2016 Abstract Book, 220 - 225, EnglishThe epigenetic regulation of FLC expression by vernalization in Brassica rapa L
- Oct. 2016, Brassica 2016 Abstract Book, 166 - 171, EnglishCharacterization of early developmental and yield heterosis in Chinese cabbage
- Inter-specific hybrids are a useful source for increasing genetic diversity. Some reproductive barriers before and/or after fertilization prevent production of hybrid plants by inter-specific crossing. Therefore, techniques to overcome the reproductive barrier have been developed, and have contributed to hybridization breeding. In recent studies, identification of molecules involved in plant reproduction has been studied to understand the mechanisms of reproductive barriers. Revealing the molecular mechanisms of reproductive barriers may allow us to overcome reproductive barriers in inter-specific crossing, and to efficiently produce inter-specific hybrids in cross-combinations that cannot be produced through artificial techniques. Inter-specific hybrid plants can potentially serve as an elite material for plant breeding, produced through the merging of genomes of parental species by allopolyploidization. Allopolyploidization provides some benefits, such as heterosis, increased genetic diversity and phenotypic variability, which are caused by dynamic changes of the genome and epigenome. Understanding of allopolyploidization mechanisms is important for practical utilization of inter-specific hybrids as a breeding material. This review discusses the importance of reproductive barriers and the effect of allopolyploidization in crop breeding programs.JAPANESE SOC BREEDING, Jun. 2016, BREEDING SCIENCE, 66(3) (3), 333 - 349, English[Refereed]Scientific journal
- 医学生物学電子顕微鏡技術学会, Mar. 2016, J. Electr. Microsc.Technol. Med. Biol., 29(2) (2), 1 - 7, EnglishUltrastructural Changes in the stigma and upper style during floral development of Japanese pear.[Refereed]Scientific journal
- Chinese cabbage (Brassica rapa L. var. pekinensis) is an important vegetable in Asia. Most Japanese commercial cultivars of Chinese cabbage use an F1 hybrid seed production system because of the high yielding cultivars produced. An efficient method for predicting hybrid performance in the parental generations is desired, and genetic distance between parental lines might be a good indicator of the level of hybrid vigor in a cross. Information concerning the genetic relationships among parental candidate inbred lines is useful for variety protection. The number of DNA markers available that can be used to assess the purity of inbred lines is limited in B. rapa. The aim of this study is to use DNA markers to assess the genetic distance between inbred lines to examine early developmental and yield heterosis so as to develop methods for selecting the best parental lines for the production of hybrids. We screened highly polymorphic SSR and CAPS markers to assess the genetic uniformity of inbred lines and characterize their genetic relationship. We examined the early size and yield heterosis in 32 F1 hybrids of Chinese cabbage. There was a moderate correlation in mid-parent heterosis between leaf size at 21 days after sowing and harvested biomass but not in best-parent heterosis. In contrast there was no correlation between genetic distance and mid-parent or best-parent heterosis, indicating that genetic distance does not predict the heterosis phenotype.Elsevier B.V., Mar. 2016, Plant Gene, 5, 1 - 7, English[Refereed]Scientific journal
- Chinese cabbage (Brassica rapa L. var. pekinensis) is an important vegetable in Asia, and most Japanese commercial cultivars of Chinese cabbage use an F1 hybrid seed production system. Self-incompatibility is successfully used for the production of F1 hybrid seeds in B. rapa vegetables to avoid contamination by non-hybrid seeds, and the strength of self-incompatibility is important for harvesting a highly pure F1 seeds. Prediction of agronomically important traits such as disease resistance based on DNA markers is useful. In this dataset, we identified the S haplotypes by DNA markers and evaluated the strength of self-incompatibility in Chinese cabbage inbred lines. The data described the predicted disease resistance to Fusarium yellows or clubroot in 22 Chinese cabbage inbred lines using gene associated or gene linked DNA markers.Elsevier Inc., Mar. 2016, Data in Brief, 6(6) (6), 229 - 237, English[Refereed]Scientific journal
- Background: Heterosis or hybrid vigour is a phenomenon in which hybrid progeny exhibit superior performance compared to their parental inbred lines. Most commercial Chinese cabbage cultivars are F-1 hybrids and their level of hybrid vigour is of critical importance and is a key selection criterion in the breeding system. Results: We have characterized the heterotic phenotype of one F-1 hybrid cultivar of Chinese cabbage and its parental lines from early- to late-developmental stages of the plants. Hybrid cotyledons are larger than those of the parents at 4 days after sowing and biomass in the hybrid, determined by the fresh weight of leaves, is greater than that of the larger parent line by approximately 20 % at 14 days after sowing. The final yield of the hybrid harvested at 63 days after sowing is 25 % greater than the yield of the better parent. The larger leaves of the hybrid are a consequence of increased cell size and number of the photosynthetic palisade mesophyll cells and other leaf cells. The accumulation of plant hormones in the F-1 was within the range of the parental levels at both 2 and 10 days after sowing. Two days after sowing, the expression levels of chloroplast-targeted genes in the cotyledon cells were upregulated in the F-1 hybrid relative to their mid parent values. Shutdown of chlorophyll biosynthesis in the cotyledon by norflurazon prevented the increased leaf area in the F-1 hybrid. Conclusions: In the cotyledons of F-1 hybrids, chloroplast-targeted genes were upregulated at 2 days after sowing. The increased activity levels of this group of genes suggested that their differential transcription levels could be important for establishing early heterosis but the increased transcription levels were transient. Inhibition of the photosynthetic process in the cotyledon reduced heterosis in later seedling stages. These observations suggest early developmental events in the germinating seedling of the hybrid may be important for later developmental vigour and yield advantage.BIOMED CENTRAL LTD, Feb. 2016, BMC PLANT BIOLOGY, 16(1) (1), 45, English[Refereed]Scientific journal
- Fusarium oxysporum f.sp. conlutinans (Foc) is a serious root-invading and xylem-colonizing fungus that causes yellowing in Brassica oleracea. To comprehensively understand the interaction between F oxysporum and B. oleracea, composition of the xylem sap proteome of the non-infected and Foc-infected plants was investigated in both resistant and susceptible cultivars using liquid chromatography-tandem mass spectrometry (LC-MS/MS) after in-solution digestion of xylem sap proteins. Whole genome sequencing of Foc was carried out and generated a predicted Foc protein database. The predicted Foc protein database was then combined with the public B. oleracea and B. rapa protein databases downloaded from Uniprot and used for protein identification. About 200 plant proteins were identified in the xylem sap of susceptible and resistant plants. Comparison between the non-infected and Foc-infected samples revealed that Foc infection causes changes to the protein composition in B. oleracea xylem sap where repressed proteins accounted for a greater proportion than those of induced in both the susceptible and resistant reactions. The analysis on the proteins with concentration change 2-fold indicated a large portion of up- and down-regulated proteins were those acting on carbohydrates. Proteins with leucine-rich repeats and legume lectin domains were mainly induced in both resistant and susceptible system, so was the case of thaumatins. Twenty-five Foc proteins were identified in the infected xylem sap and 10 of them were cysteine-containing secreted small proteins that are good candidates for virulence and/or avirulence effectors. The findings of differential response of protein contents in the xylem sap between the non-infected and Foc-infected samples as well as the Foc candidate effectors secreted in xylem provide valuable insights into B. oleracea-Foc interactions.FRONTIERS MEDIA SA, Feb. 2016, FRONTIERS IN PLANT SCIENCE, 7, 31, English[Refereed]Scientific journal
- Epigenetic regulation is crucial for the development of plants and for adaptation to a changing environment. Recently, genome-wide profiles of histone modifications have been determined by a combination of chromatin immunoprecipitation (ChIP) and genomic tiling arrays (ChIP on chip) or ChIP and high-throughput sequencing (ChIP-seq) in species including Arabidopsis thaliana, rice and maize. Validation of ChIP analysis by PCR or qPCR using positive and negative regions of histone modification is necessary. In contrast, information about histone modifications is limited in Chinese cabbage, Brassica rapa. The aim of this study was to develop positive and negative control primer sets for H3K4me3 (trimethylation of the 4th lysine of H3), H3K9me2, H3K27me3 and H3K36me3 in B. rapa. The expression and histone modification of four FLC paralogs in B. rapa, before and after vernalization, were examined using the method developed here. After vernalization, expression of all four BrFLC genes was reduced, and accumulation of H3K27me3 was observed in three of them. As with A. thaliana, the vernalization response and stability of FLC repression correlated with the accumulation of H3K27me3. These results suggest that the epigenetic state during vernalization is important for high bolting resistance in B. rapa. The positive and negative control primer sets developed here revealed positive and negative histone modifications in B. rapa that can be used as a control for future studies.GENETICS SOC JAPAN, Feb. 2016, GENES & GENETIC SYSTEMS, 91(1) (1), 1 - 10, English[Refereed]Scientific journal
- We identified the candidate gene conferring yellow wilt resistance (YR) in B. oleracea . This work will facilitate YR breeding programs for B. oleracea and its closely related species. Yellow wilt disease is one of the most serious diseases of cabbage worldwide. Type A resistance to the disease is controlled by a single dominant gene that is used in cabbage breeding. Our previous QTL study identified the FocBo1 locus controlling type A resistance. In this study, the FocBo1 locus was fine-mapped by using 139 recombinant F-2 plants derived from resistant cabbage (AnjuP01) and susceptible broccoli (GCP04) DH lines. As a result, we successfully delimited the location of FocBo1 within 1.00 cM between markers, BoInd 2 and BoInd 11. Analysis of BAC and cosmid sequences corresponding to the FocBo1 locus identified an orthologous gene of Bra012688 that was recently identified as an candidate gene that confers yellows resistance in Chinese cabbage. The candidate gene-specific DNA markers and phenotypes in F-1 cabbage cultivars and their selfed F-2 populations showed a perfect correlation. Our identification of the candidate gene for FocBo1 will assist introduction of fusarium resistance into B. oleracea cultivars and contribute further understanding of interaction between Brassica plants and fusarium.SPRINGER, Jan. 2015, THEORETICAL AND APPLIED GENETICS, 128(1) (1), 119 - 130, English[Refereed]Scientific journal
- Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans is an important disease of Brassica worldwide. To identify a resistance (R) gene against Fusarium yellows in Chinese cabbage (Brassica rapa var. pekinensis), we analyzed differential expression at the whole genome level between resistant and susceptible inbred lines using RNA sequencing. Four hundred and eighteen genes were significantly differentially expressed, and these were enriched for genes involved in response to stress or stimulus. Seven dominant DNA markers at putative R-genes were identified. Presence and absence of the sequence of the putative R-genes, Bra012688 and Bra012689, correlated with the resistance of six inbred lines and susceptibility of four inbred lines, respectively. In F-2 populations derived from crosses between resistant and susceptible inbred lines, presence of Bra012688 and Bra012689 cosegregated with resistance, suggesting that Bra012688 and Bra012689 are good candidates for fusarium yellows resistance in Chinese cabbage.SPRINGER, Jun. 2014, PLANT MOLECULAR BIOLOGY, 85(3) (3), 247 - 257, English[Refereed]Scientific journal
- 日本育種学会, Jun. 2014, 育種学研究, 16(2) (2), 79 - 85, Japanese[Invited]
- Brassica napus is a leading oilseed crop throughout many parts of the world. It is well adapted to long day photoperiods, however, it does not adapt well to short day subtropical regions. Short duration B. nap us plants were resynthesized through ovary culture from interspecific crosses in which B. rapa cultivars were reciprocally crossed with B. oleracea. From five different combinations, 17 hybrid plants were obtained in both directions. By self-pollinating the F-1 hybrids or introgressing them with cultivated B. napus, resynthesized (RS) F-3 and semi-resynthesized (SRS) F-2 generations were produced, respectively. In field trial in Bangladesh, the RS B. napus plants demonstrated variation in days to first flowering ranging from 29 to 73 days; some of which were similar to cultivated short duration B. napus, but not cultivated short duration B. rapa. The RS and SRS B. napus lines produced 2-4.6 and 1.6-3.7 times higher yields, respectively, as compared to cultivated short duration B. napus. Our developed RS lines may be useful for rapeseed breeding not only for subtropical regions, but also for areas such as Canada and Europe where spring rapeseed production can suffer from late spring frosts. Yield and earliness in RS lines are discussed.JAPANESE SOC BREEDING, Jan. 2014, BREEDING SCIENCE, 63(5) (5), 495 - 502, English[Refereed]Scientific journal
- Throughout the world, clubroot disease is one of the most damaging diseases affecting Brassica oleracea. To develop marker-assisted selection (MAS) that could assist the incorporation of durable clubroot resistance (CR) into cultivars, previous genetic analyses have identified several CR quantitative trait loci (CR-QTL). However, the independent and cumulative effects of each CR locus against various isolates have rarely been tested. Previously, we identified one major CR-QTL and four minor CR-QTL in the F2 plants from broccoli doubled haploid (DH) line x cabbage DH line of B. oleracea. In the present study, to clarify their effectiveness for controlling disease involving various isolates, inoculation testing was conducted in genotypes with various combinations of the CR genes, which were selected using the DNA markers closely associated with each CR-QTL. In exploring the overall disease incidence, it was apparent that a single involvement of the major CR gene located in the PbBo(Anju)1 locus, or accumulation of CR genes in the minor CR-QTL, is not enough to confer sufficient resistance. One major CR gene in the QTL PbBo(Anju)1 locus plus two to three minor CR genes conferred moderate resistance. The genotype in which all of the CR genes locating in the five QTL including PbBo(Anju)1 were accumulated showed the highest resistance, and it was broadly resistant against six isolates. Accumulation of several CR genes by MAS is necessary to conduct CR breeding in B. oleracea. Our developed DNA markers can be used efficiently to make selections of required loci for the acquisition of resistance, and use of these markers will be a powerful tool for CR breeding in B. oleracea.SPRINGER, Dec. 2013, MOLECULAR BREEDING, 32(4) (4), 889 - 900, English[Refereed]Scientific journal
- Hybrid vigour, or heterosis, refers to the increased yield and biomass of hybrid offspring relative to the parents. Although this has been exploited in plants for agriculture and horticulture, the molecular and cellular mechanisms underlying hybrid vigour are largely unknown. Genetic analyses show that there are a large number of quantitative trait loci (QTLs) that contribute to the heterotic phenotype, indicating that it is a complex phenomenon. Gene expression in hybrids is regulated by the interactions of the two parental epigenetic systems and the underlying genomes. Increasing understanding of the interplay of small RNA (sRNA) molecules, DNA methylation, and histone marks provides new opportunities to define the basis of hybrid vigour and to understand why F1 heterosis is not passed on to subsequent generations. We discuss recent findings that suggest the existence of several pathways that alter DNA methylation patterns, which may lead to transcriptional changes resulting in the heterotic phenotype.ELSEVIER SCIENCE LONDON, Dec. 2013, TRENDS IN GENETICS, 29(12) (12), 684 - 690, English[Refereed]Scientific journal
- Comparison of Positions of QTLs Conferring Resistance to Xanthomonas campestris pv. campestris in Brassica oleraceaBlack rot, caused by Xanthomonas campestris pv. campestris (Xcc) is possibly the most important disease of Brassica worldwide. To compare chromosomal positions of Xcc resistance loci in Brassica oleracea between the present and published studies and to develop marker assisted selection (MAS) to resistance against Xcc race 1, we constructed a B. oleracea map, including pW, pX anScientific Research, Aug. 2013, American Journal of Plant Sciences, 4(8) (8), 11 - 20, English[Refereed]Scientific journal
- 2013, DNA Methylation: Principles, Mechanisms and Challenges[Refereed]
- Difference in the level of expression of genes is one of the factors contributing to plant phenotype. As well as being under genetic control, gene expression is regulated by epigenetic processes such as DNA methylation and histone modifications. We compared genome-wide gene expression between Arabidopsis thaliana and the related species Arabidopsis lyrata subsp. lyrata and Arabidopsis halleri subsp. gemmifera to understand which genes might contribute to species differences. Genes categorized into response to stress tended to show differential expression between species, suggesting that divergence of expression in these genes contributes to adaptation to environmental conditions following the divergence of species. Regions methylated in A. lyrata were identified from Methylated-DNA immunoprecipitation, and this DNA methylation profile together with transcriptome data revealed that gene body methylation was not associated with differential gene expression between A. thaliana and A. lyrata. The DNA methylation status of four putative imprinted genes of A. lyrata was examined and found to be conserved in vegetative tissues between A. thaliana and A. lyrata in FIS2, HDG3, and HDG9, but not in HDG8. (C) 2012 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, Sep. 2012, GENE, 506(2) (2), 301 - 309, English[Refereed][Invited]Scientific journal
- Fusarium wilt caused by Fusarium oxysporum f. sp. conglutinans is one of the most important diseases of Brassica crops, resulting in severe reductions in yield and quality. To characterize the inheritance pattern of fusarium resistance, a cross between a susceptible broccoli and a resistant cabbage was subjected to segregation analysis. Results indicated that resistance was controlled by a single dominant allele. This gene was named Foc-Bo1 and mapped to linkage group seven (O7) by both the segregation test and quantitative trait locus (QTL) analysis. The QTL on O7 was detected with a logarithm of odds score (LOD) of 19.5, which was above the threshold value with genome-wide 1% significance level (2.01). A minor QTL was also detected on O4 with a LOD score of 2.06. Inoculation tests indicated that stable expression of fusarium resistance at high temperatures required Foc-Bo1 homozygosity. The association between Foc-Bo1 and the closest simple sequence repeat marker (KBrS003O1N10) was analyzed in three F3 populations. Based on these studies, KBrS003O1N10 represents an effective marker-assisted selection (MAS) tool for breeding fusarium wilt resistance into Brassica oleracea crops. To our knowledge, this is the first paper to map the fusarium-resistance gene in Brassica species and to validate the effectiveness of MAS in improving fusarium resistance in these important plants.SPRINGER, Aug. 2012, MOLECULAR BREEDING, 30(2) (2), 809 - 818, English[Refereed]Scientific journal
- Natural variation is defined as the phenotypic variation caused by spontaneous mutations. In general, mutations are associated with changes of nucleotide sequence, and many mutations in genes that can cause changes in plant development have been identified. Epigenetic change, which does not involve alteration to the nucleotide sequence, can also cause changes in gene activity by changing the structure of chromatin through DNA methylation or histone modifications. Now there is evidence based on induced or spontaneous mutants that epigenetic changes can cause altering plant phenotypes. Epigenetic changes have occurred frequently in plants, and some are heritable or metastable causing variation in epigenetic status within or between species. Therefore, heritable epigenetic variation as well as genetic variation has the potential to drive natural variation.MDPI AG, Aug. 2012, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 13(8) (8), 9900 - 9922, English[Refereed][Invited]Scientific journal
- An allopolyploid is an individual having two or more complete sets of chromosomes derived from different species. Generation of allopolyploids might be rare because of the need to overcome limitations such as co-existing populations of parental lines, overcoming hybrid incompatibility, gametic non-reduction, and the requirement for chromosome doubling. However, allopolyploids are widely observed among plant species, so allopolyploids have succeeded in overcoming these limitations and may have a selective advantage. As techniques for making allopolyploids are developed, we can compare transcription, genome organization, and epigenetic modifications between synthesized allopolyploids and their direct parental lines or between several generations of allopolyploids. It has been suggested that divergence of transcription caused either genetically or epigenetically, which can contribute to plant phenotype, is important for the adaptation of allopolyploids.MDPI, Jul. 2012, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 13(7) (7), 8696 - 8721, English[Refereed][Invited]Scientific journal
- Arabidopsis thaliana shows hybrid vigor (heterosis) in progeny of crosses between Columbia-0 and C24 accessions. Hybrid vigor was evident as early as mature seeds and in seedlings 3 d after sowing (DAS). At 3 DAS, genes encoding chloroplast-located proteins were significantly overrepresented (187) among the 724 genes that have greater than midparent values of expression in the hybrid. Many of these genes are involved in chlorophyll biosynthesis and photosynthesis. The rate of photosynthesis was constant per unit leaf area in parents and hybrids. Larger cell sizes in the hybrids were associated with more chloroplasts per cell, more total chlorophyll, and more photosynthesis. The increased transcription of the chloroplast-targeted genes was restricted to the 3-7 DAS period. At 10 DAS, only 118 genes had expression levels different from the expected midparent value in the hybrid, and only 12 of these genes were differentially expressed at 3 DAS. The early increase in activity of genes involved in photosynthesis and the associated phenomena of increases in cell size and number through development, leading to larger leaf areas of all leaves in the hybrid, suggest a central role for increased photosynthesis in the production of the heterotic biomass. In support of this correlation, we found that an inhibitor of photosynthesis eliminated heterosis and that higher light intensities enhanced both photosynthesis and heterosis. In hybrids with low-level heterosis (Landsberg erecta x Columbia-0), chloroplast-targeted genes were not up-regulated and leaf areas were only marginally increased.NATL ACAD SCIENCES, May 2012, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(18) (18), 7109 - 7114, English[Refereed][Invited]Scientific journal
- 2012, Bulletin of the Faculty of Agriculture, Niigata University, 64(2) (2), 143 - 149[Refereed]
- The merging of two different genomes occurs during the formation of amphidiploids, and the merged regulatory networks have the potential to generate a new gene expression pattern. We examined the genome-wide gene expression of two newly synthesized amphidiploids between Arabidopsis thaliana and the related species Arabidopsis lyrata subsp. lyrata and Arabidopsis halleri subsp. gemmifera. 1,137 (4.7%) and 1,316 (5.4%) of probesets showed differential gene expression in A. thaliana-A. halleri and A. thaliana-A. lyrata hybrids respectively, compared to the mid parent value and of these, 489 were in common. Genes that differed in expression between the parental lines tended to have an expression level in both hybrids differing from the mid parent value. In contrast to protein coding genes, there is little differential expression of transposons. Genes in the categories of chloroplast-targeted and response to stress were overrepresented in the non-additively expressed genes in both amphidiploids. As these genes have the potential to contribute directly to the plant phenotype, we suggest that rapid changes of gene expression in amphidiploids might be important for producing greater biomass.SPRINGER, Nov. 2011, PLANT MOLECULAR BIOLOGY, 77(4-5) (4-5), 419 - 431, English[Refereed]Scientific journal
- Arabidopsis thaliana is a quantitative long-day plant with the timing of the floral transition being regulated by both endogenous signals and multiple environmental factors, fwa is a late-flowering mutant, and this phenotype is due to ectopic FWA expression caused by hypomethylation at the FWA locus. The floral transition results in the activation of the floral development process, the key regulators being the floral meristem identity genes, AP1 (APETALA1) and LFY (LEAFY). In this study, we describe inflorescence abnormalities in plants overexpressing the Arabidopsis lyrata FT (AIFT) and A. thaliana FWA (AtFWA) genes simultaneously. The inflorescence abnormality phenotype was present in only a proportion of plants. All plants overexpressing both AIFT and AtFWA flowered earlier than fwa, suggesting that the inflorescence abnormality and earlier flowering time are caused independently. The inflorescence abnormality phenotype was similar to that of the double mutant of ap1 and fly, and AP1 and LFY genes were down-regulated in the abnormal inflorescences. From these results, we suggest that not only does ectopic AtFWA expression inhibit AtFT/AIFT function to delay flowering but that overexpression of AtFWA and AIFT together inhibits AP1 and LFY function to produce abnormal inflorescences. (C) 2011 Elsevier Ireland Ltd. All rights reserved.ELSEVIER IRELAND LTD, Oct. 2011, PLANT SCIENCE, 181(4) (4), 496 - 503, English[Refereed]Scientific journal
- In this review we have analysed two major biological systems involving epigenetic control of gene activity. In the first system we demonstrate the interplay between genetic and epigenetic controls over the transcriptional activity of FLC, a major repressor of flowering in Arabidopsis. FLC is down-regulated by low temperature treatment (vernalisation) releasing the repressor effect on flowering. We discuss the mechanisms of the reduced transcription and the memory of the vernalisation treatment through vegetative development. We also discuss the resetting of the repressed activity level of the PLC gene, following vernalisation, to the default high activity level and show it occurs during both male and female gametogenesis but with different timing in each. In the second part of the review discussed the complex multigenic system which is responsible for the patterns of gene activity which bring about hybrid vigour in crosses between genetically similar but epigenetically distinct parents. The epigenetic systems that we have identified as contributing to the heterotic phenotype are the 24nt siRNAs and their effects on RNA dependent DNA methylation (RdDM) at the target loci leading to changed expression levels. We conclude that it is likely that epigenetic controls are involved in expression systems in many aspects of plant development and plant function. (C) 2011 Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, Aug. 2011, BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, 1809(8) (8), 427 - 437, English[Refereed]Scientific journal
- P>fwa is a late flowering epi-mutant in Arabidopsis thaliana. FWA is silenced by DNA methylation in vegetative tissue but is demethylated in the central cell of the female ovule and continues to be expressed in the endosperm from the maternal copy. FWA is stably silenced in A. thaliana, but in related Arabidopsis species, FWA expression and DNA methylation levels vary in vegetative tissue. In this study, we show that variation in FWA expression in field isolates having identical DNA sequences is associated with changes in DNA methylation and may change over time. Vegetative FWA expression is correlated with decreased methylation at non-CG sites in the region upstream of the transcription start site in species related to A. thaliana and we conclude that methylation of this region is critical for FWA silencing in these species. In A. thaliana, FWA expression is affected by methylation in regions both upstream and downstream of the transcription start site. Ectopic A. thaliana FWA expression causes a late flowering phenotype, but over-expression of Arabidopsis lyrata FWA does not. In A. thaliana, stable silencing of FWA to prevent late flowering may have evolved through the selection of large tandem repeats and spread of the critical methylated region to include these repeats.WILEY-BLACKWELL, Jun. 2011, PLANT JOURNAL, 66(5) (5), 831 - 843, English[Refereed]Scientific journal
- DNA methylation is an important epigenetic modification regulating gene expression and transposon silencing. Although epigenetic regulation is involved in some agricultural traits, there has been relatively little research on epigenetic modifications of genes in Brassica rapa, which includes many important vegetables. In B. rapa, orthologs of DDM1, a chromatin remodeling factor required for maintenance of DNA methylation, have been characterized and DNA hypomethylated knock-down plants by RNAi (ddm1-RNAi plants) have been generated. In this study, we investigated differences of DNA methylation status at the genome-wide level between a wild-type (WT) plant and a ddm1-RNAi plant by methylation-sensitive amplification polymorphism (MSAP) analysis. MSAP analysis detected changes of DNA methylation of many repetitive sequences in the ddm1-RNAi plant. Search for body methylated regions in the WT plant revealed no difference in gene body methylation levels between the WT plant and the ddm1-RNAi plant. These results indicate that repetitive sequences are preferentially methylated by DDM1 genes in B. rapa.SPRINGER, Jan. 2011, PLANT CELL REPORTS, 30(1) (1), 81 - 88, English[Refereed]Scientific journal
- A central question in genomic imprinting is how a specific sequence is recognized as the target for epigenetic marking. In both mammals and plants, imprinted genes are often associated with tandem repeats and transposon-related sequences, but the role of these elements in epigenetic gene silencing remains elusive. FWA is an imprinted gene in Arabidopsis thaliana expressed specifically in the female gametophyte and endosperm. Tissue-specific and imprinted expression of FWA depends on DNA methylation in the FWA promoter, which is comprised of two direct repeats containing a sequence related to a SINE retroelement. Methylation of this element causes epigenetic silencing, but it is not known whether the methylation is targeted to the SINE-related sequence itself or the direct repeat structure is also necessary. Here we show that the repeat structure in the FWA promoter is highly diverse in species within the genus Arabidopsis. Four independent tandem repeat formation events were found in three closely related species. Another related species, A. halleri, did not have a tandem repeat in the FWA promoter. Unexpectedly, even in this species, FWA expression was imprinted and the FWA promoter was methylated. In addition, our expression analysis of FWA gene in vegetative tissues revealed high frequency of intra-specific variation in the expression level. In conclusion, we show that the tandem repeat structure is dispensable for the epigenetic silencing of the FWA gene. Rather, SINE-related sequence is sufficient for imprinting, vegetative silencing, and targeting of DNA methylation. Frequent independent tandem repeat formation events in the FWA promoter led us to propose that they may be a consequence, rather than cause, of the epigenetic control. The possible significance of epigenetic variation in reproductive strategies during evolution is also discussed.PUBLIC LIBRARY SCIENCE, Apr. 2008, PLOS GENETICS, 4(4) (4), English[Refereed]Scientific journal
- A central question in genomic imprinting is how a specific sequence is recognized as the target for epigenetic marking. In both mammals and plants, imprinted genes are often associated with tandem repeats and transposon-related sequences, but the role of these elements in epigenetic gene silencing remains elusive. FWA is an imprinted gene in Arabidopsis thaliana expressed specifically in the female gametophyte and endosperm. Tissue-specific and imprinted expression of FWA depends on DNA methylation in the FWA promoter, which is comprised of two direct repeats containing a sequence related to a SINE retroelement. Methylation of this element causes epigenetic silencing, but it is not known whether the methylation is targeted to the SINE-related sequence itself or the direct repeat structure is also necessary. Here we show that the repeat structure in the FWA promoter is highly diverse in species within the genus Arabidopsis. Four independent tandem repeat formation events were found in three closely related species. Another related species, A. halleri, did not have a tandem repeat in the FWA promoter. Unexpectedly, even in this species, FWA expression was imprinted and the FWA promoter was methylated. In addition, our expression analysis of FWA gene in vegetative tissues revealed high frequency of intra-specific variation in the expression level. In conclusion, we show that the tandem repeat structure is dispensable for the epigenetic silencing of the FWA gene. Rather, SINE-related sequence is sufficient for imprinting, vegetative silencing, and targeting of DNA methylation. Frequent independent tandem repeat formation events in the FWA promoter led us to propose that they may be a consequence, rather than cause, of the epigenetic control. The possible significance of epigenetic variation in reproductive strategies during evolution is also discussed. © 2008 Fujimoto et al.Apr. 2008, PLoS Genetics, 4(4) (4), e1000048, English[Refereed]Scientific journal
- DNA methylation and histone modification play important roles in regulating gene expression. The DDM1 gene in Arabidopsis thaliana (AtDDM1) is required for the maintenance of DNA methylation level and histone H3 methylation pattern. We isolated DDM1 homologs of Brassica rapa, BrDDM1a and BrDDM1b, which have 84.4% and 84.1% deduced amino acid sequence identities with AtDDM1, respectively. Both the BrDDM1a and BrDDM1b genes were found to be expressed in vegetative and reproductive tissues. B. rapa ddm1-RNAi transgenic plants with reduced levels of BrDDM1a/BrDDM1b expression showed genome-wide and non-tissue-specific demethylation. These results suggest that the BrDDM1a and BrDDM1b genes are orthologs of AtDDM1 and are required for the maintenance of DNA methylation as is AtDDM1. Despite genome-wide demethylation, developmental abnormalities were not found in the ddm1-RNAi transgenic plants. Dominance relationships of SP11/SCR alleles, the determinant of pollen recognition specificity in Brassica self-incompatibility, in S heterozygotes in B. rapa were not influenced by the low level of the BrDDM1 expression. Transcriptional reactivation of retrotransposon-like sequences observed in the ddm1-RNAi transgenic plants indicates that BrDDM1a and BrDDM1b participate in silencing of retrotransposons. Hypomethylation states of the ddm1-RNAi transgenic plants were inherited by plants of the next generation even by plants which had lost the RNAi construct by segregation. Remethylation was observed in a few progenies. Efficiencies of remethylation in the progenies without the RNAi construct were different between 18S rDNA, BoSTF12a/15a, and BrTto1 sequences.SPRINGER, Mar. 2008, PLANT MOLECULAR BIOLOGY, 66(5) (5), 463 - 473, English[Refereed]Scientific journal
- The pattern of amplification and differentiation of Ty1-copia and Ty3-gypsy retrotransposons in Brassicaceae speciesOne of the causes of genome size expansion is considered to be amplification of retrotransposons. We determined nucleotide sequences of 24 PCR products for each of six retrotransposons in Brassica rapa and Brassica oleracea. Phylogenetic trees of these sequences showed species-specific clades. We also sequenced STF7a homologs and Tto1 homologs, 24 PCR products each, in nine diploids and three allopolyploids, and constructed phylogenetic trees. In these phylogenetic trees, species-specific clades of diploid species were also formed, but retrotransposons of allopolyploids were clustered into the clades of their original genomes, indicating that these two retrotransposons amplified after speciation of the nine diploids. Genetic variation in these retrotransposons may have arisen before emergence of allopolyploid species. There was a positive correlation between the genome size and the average number of substitutions of STF7a and Tto1 homologs in at least seven diploids. The implications of these results in the genome evolution of Brassicaceae are herein discussed.GENETICS SOC JAPAN, Feb. 2008, GENES & GENETIC SYSTEMS, 83(1) (1), 13 - 22, English[Refereed]Scientific journal
- Molecular imprinting is the differential expression and/or silencing of alleles according to their parent of origin [1, 2]. Conflicts between parents, or parents and offspring, should cause "arms races," with accelerated evolution of the genes involved in imprinting. This should be detectable in the evolution of imprinting genes' protein sequences and in the promoter regions of imprinted genes. Previous studies, however, found no evidence of more amino acid substitutions in imprinting genes [1, 3]. We have analyzed sequence diversity of the Arabidopsis lyrata Medea (MEA) gene and divergence from the A. thaliana sequence, including the first study of the promoter region. In A. thaliana, MEA is imprinted, with paternal alleles silenced in endosperm cells [4, 5], and also functions in the imprinting machinery [4, 6]; MEA protein binding at the MEA promoter region indicates self-regulated imprinting [7-9]. We find the same paternal MEA allele silencing in A. lyrata endosperm but no evidence for adaptive evolution in the coding region, whereas the 5' flanking region displays high diversity, with distinct haplotypes, suggesting balancing selection in the promoter region.CELL PRESS, Nov. 2007, CURRENT BIOLOGY, 17(21) (21), 1885 - 1889, English[Refereed]Scientific journal
- In self-incompatibility, a number of S haplotypes are maintained by frequency-dependent selection, which results in trans-specific S haplotypes. The region of several kilobases (similar to 40-60 kb) from SP6 to SP2, including self-incompatibility-related genes and some adjacent genes in Brassica rapa, has high nucleotide diversity due to the hitchhiking effect, and therefore we call this region the "S-locus complex." Recombination in the S-locus complex is considered to be suppressed. We sequenced regions of >50 kb of the S-locus complex of three S haplotypes in B. rapa and found higher nucleotide diversity in intergenic regions than in coding regions. Two highly similar regions of >10 kb were found between BrS-8 and BrS-46 Phylogenetic analysis using trans-specific S haplotypes (called interspecific pairs) of B. rapa and B. oleracea suggested that recombination reduced the nucleotide diversity in these two regions and that the genes not involved in self-incompatibility in the S-locus complex and the kinase domain, but not the S domain, of SRK have also experienced recombination. Recombination may reduce hitchhiking diversity in the S-locus complex, whereas the region from the S domain to SP11 would disfavor recombination.GENETICS, Oct. 2007, GENETICS, 177(2) (2), 949 - 958, English[Refereed]Scientific journal
- DNA polymorphism of the Wx gene in glutinous rice cultivars was investigated by PCR-RF-SSCP and heteroduplex cleavage analysis using Brassica petiole extract, and the nucleotide sequence variations were identified. Most japonica-type glutinous rice was found to have a 23-bp duplication in the second exon, which causes loss of the function of granule-bound starch synthase (GBSS) encoded by the Wx gene. Without the 23-bp duplication, there was an insertion of 7,764 bp in the ninth exon of the wx allele of 'Oragamochi'. Expression analysis of the wx allele using RT-PCR and Northern blot analysis revealed that transcripts of the 'Oragamochi' wx allele are about 1-kb shorter and that the deduced amino acid sequence of the transcript lacks a motif important for GBSS. Therefore, this insertion was considered to be the cause of the glutinous trait of 'Oragamochi'. This 7,764-bp insertion had long terminal repeats, a primer binding site, and a polypurine tract, but no sequence homologous with gag and pol, suggesting that it is a non-autonomous element. Furthermore, it had a structure similar to Dasheng and may be a member of Dasheng.SPRINGER, Jul. 2007, THEORETICAL AND APPLIED GENETICS, 115(2) (2), 217 - 224, English[Refereed]Scientific journal
- Brassica napus is an amphidiploid species with the A genome from Brassica rapa and the C genome from Brassica oleracea. Although B. rapa, B. oleracea and artificially synthesized amphidiploids with the AC genome are self-incompatible, B. napus is self-compatible. Six S genotypes were identified in B. napus, five of which had class I S haplotypes from one species and a class II S haplotype from the other species, and mutations causing self-compatibility were identified in three of these S genotypes. The most predominant S genotype (BnS-1;BnS-6), which is that of cv. 'Westar', had a class I S haplotype similar to B. rapa S-47 (BrS-47) and a class II S haplotype similar to B. oleracea S-15 (BoS-15). The stigmas of 'Westar' rejected the pollen grains of both BrS-47 and BoS-15, while reciprocal crossings were compatible. Insertion of a DNA fragment of about 3.6 kb was found in the promoter region of the SP11/SCR allele of BnS-1, and transcripts of SP11/SCR were not detected in 'Westar'. The nucleotide sequence of the SP11 genomic DNA of BnS-6 was 100% identical to that of SP11 of BoS-15. Class I SP11 alleles from one species showed dominance over class II SP11 alleles from the other species in artificially synthesized B. napus lines, suggesting that the non-functional dominant SP11 allele suppressed the expression of the recessive SP11 allele in 'Westar'. Two other S genotypes in B. napus also had non-functional class I S haplotypes together with recessive BnS-6. These observations suggest independent origins of self-compatibility in B. napus.BLACKWELL PUBLISHING, May 2007, PLANT JOURNAL, 50(3) (3), 391 - 400, English[Refereed]Scientific journal
- Self-incompatibility system has been most intensively studied in Brassica because of the advantage as plant materials and the importance as crop species. The determinants of self-recognition specificity in the stigma and the pollen are SRK and SP11/SCR, respectively. The genes of SRK and SP11 have many alleles in a species and are closely linked with each other in the S-locus complex. A set of SRK and SP11 alleles are referred to as S haplotype. Sequence analyses of many alleles of SRK and SP11 in Brassica species revealed pairs of S haplotypes having highly similar SRK and SP11 alleles between different species. The interspecific pair of S haplotypes is considered to be derived from the same ancestral S haplotype and to have maintained the same recognition specificity. The structure of the S-locus complex is highly variable, and this high structural polymorphism is considered to be important for suppressing the recombination between SRK and SP11, which may result in the breakdown of self-incompatibility. The first candidate protein identified as the female S determinant was SLG, which is abundant on the stigma surface, but the function of SLG in self-incompatibility is unclear. The dominance relationships of the SRK alleles are different from those of the SP11 alleles, and dominance order of S haplotypes is nonlinear. Recently, methylation of the promoter region of SP11 has been reported to be responsible for transcriptional suppression of recessive SP11. Several mutations in SP11 and SRK causing self-compatibility have been identified, and other genes controlling self-incompatibility have also been reported. These molecular studies can contribute to the development of the F(1) hybrid breeding using the self-incompatibility system in Brassica crops.ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD, 2007, ADVANCES IN BOTANICAL RESEARCH: INCORPORATING ADVANCES IN PLANT PATHOLOGY, VOL 45, 45, 139 - 154, English[Refereed]In book
- Characterization of DNA methyltransferase genes in Brassica rapaDNA methylation is essential for normal development and plays important roles in regulating gene expression in plants. Analysis of the key enzymes catalyzing DNA methylation is important to understand epigenetic phenomena. In this study, three putative methyltransferase genes, BrMET1a, BrMET1b, and BrCMT, were isolated from a genome library of Brassica rapa. Structural conservation of the amino acid sequence between BrMET1a/BrMET1b and AtMET1 and that between BrCMT and AtCMT3 suggests that they may function as DNA methyltransferase. BrMET1a was expressed in vegetative and reproductive organs, while BrMET1b was expressed only in pistils, indicating that these two genes have different functions. BrCMT was expressed especially in stamens at the stage of 2-4 days before anthesis. We isolated three DNA methyltransferase genes in Brassica rapa and indicated differences of expression patterns of these DNA methyltransferase genes and expression levels in different tissues and developmental stages, suggesting that these genes might play important roles in epigenetic gene regulation in B. rapa.GENETICS SOC JAPAN, Aug. 2006, GENES & GENETIC SYSTEMS, 81(4) (4), 235 - 242, English[Refereed]Scientific journal
- Mutations in the S locus of a self-compatible cultivar Yellow Sarson in Brassica rapa, which has a self-compatible class-I S haplotype, S-f2, were investigated. S-28 in Brassica oleracea was found to be a member of an interspecific pair with S-f2 in B. rapa. The original S haplotype of S-f2 was identified to be S-54 in B. rapa. Sequence comparison of alleles in S-f2 with those in S-54 and B. oleracea S-28 revealed insertion of a retrotransposon-like sequence in the first intron of SRK and 89-bp deletion in the promoter region of SP11. No transcripts of SRK and SP11 were detected in S-f2 homozygotes, suggesting that the insertion and the deletion in SRK and SP11, respectively, caused the loss of the function of these genes. Promoter assay using transgenic plants indicated that the SP11 promoter of S-f2 has no activity. Heterozygotes of S-f2 and a normal class-II S haplotype, S-60, in B. rapa were found to be self-compatible. Interestingly, transcription of SP11-60 was revealed to be suppressed in the S-f2/S-60 heterozygotes, suggesting that an untranscribed class-I SP11 allele suppresses the expression of a recessive class-II SP11 allele in the anthers of S heterozygotes. Similar phenomenon was observed in heterozygotes of a self-compatible class-I S haplotype and a self-incompatible class-II S haplotype in B. oleracea.SPRINGER, Jul. 2006, PLANT MOLECULAR BIOLOGY, 61(4-5) (4-5), 577 - 587, English[Refereed]Scientific journal
- Nuclear so-called fertility-restorer genes reverse the pollen sterility of cytoplasmic male-sterile (CMS) plants caused by disturbed mitochondrial-nuclear interactions. We identified a CMS-associated chimeric mitochondrial gene in an alloplasmic CMS line of Brassica oleracea in the 'mur' system. This novel chimeric gene, orf72, was found in the mitochondrial genome of donor cytoplasm. It was located downstream of normal rps7 and contained part of atp9 (atp9-b). It was expressed specifically on the nuclear background of CMS B. oleracea, partially suppressed in the fertility-restored line and entirely suppressed in the cytoplasmic donor.OXFORD UNIV PRESS, Apr. 2006, PLANT AND CELL PHYSIOLOGY, 47(4) (4), 549 - 553, English[Refereed]Scientific journal
- Self-compatible S-54 homozygotic plants were found in progenies of an F-1 hybrid cultivar in Chinese cabbage. Pollination tests revealed that this self-compatibility is controlled by the S locus and caused by the loss of the recognition function of the stigma. SRK, the gene for the recognition molecule in the stigma, was normally transcribed and translated in the self-compatible plants. The 1034-bp region in the receptor domain of SRK in the self-compatible plants was 100% identical to SLG in S-54, while that in self-incompatible S-54 homozygotic plants was 95.1% identical. These results suggest that the self-compatibility of the S-54 homozygotes is due to amino-acid changes caused by gene conversion from SLG to SRK. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.ELSEVIER SCIENCE BV, Jan. 2006, FEBS LETTERS, 580(2) (2), 425 - 430, English[Refereed]Scientific journal
- The determinants of recognition specificity of self-incompatibility in Brassica are SRK in the stigma and SP11/SCR in the pollen, both of which are encoded in the S locus. The nucleotide sequence analyses of many SRK and SP11/SCR alleles have identified several interspecific pairs of S haplotypes having highly similar sequences between B. oleracea and B. rapa. These interspecific pairs of S haplotypes are considered to be derived from common ancestors and to have maintained the same recognition specificity after speciation. In this study, the genome structures of three interspecific pairs of S haplotypes were compared by sequencing SRK, SP11/SCR, and their flanking regions. Regions between SRK and SP11/SCR in B. oleracea were demonstrated to be much longer than those of B. rapa and several retrotransposon-like sequences were identified in the S locus in B. oleracea. Among the seven retrotransposon-like sequences, six sequences were found to belong to the ty3 gypsy group. The gag sequences of the retrotransposon-like sequences were phylogenetically different from each other. In Southern blot analysis using retrotransposon-like sequences as probes, the B. oleracea genome showed more signals than the B. rapa genome did. These findings suggest a role for the S locus and genome evolution in self-incompatible plant species. Copyright © 2006 by the Genetics Society of America.2006, Genetics, 173(2) (2), 1157 - 1167, English[Refereed]Scientific journal
- OXFORD UNIV PRESS, 2006, PLANT AND CELL PHYSIOLOGY, 47(4) (4), S238 - S238, EnglishCharacterization of DNA methyltransferase genes in Brassica rapa[Refereed]Scientific journal
- The nucleotide sequence of an 86.4-kb region that includes the SP11, SRK, and SLG genes of Brassica rapa S-60 (a class-II S haplotype) was determined. In the sequenced region, 13 putative genes were found besides SP11-60, SRK-60, and SLG-60. Five of these sequences were isolated as cDNAs, five were homologues of known genes, cDNAs, or ORFs, and three are hypothetical ORFs. Based on their nucleotide sequences, however, some of them are thought to be non-functional. Two regions of colinearity between the class-II S-60 and Brassica class-I S haplotypes were identified, i.e., S flanking region 1 which shows partial colinearity of non-genic sequences and S flanking region 2 which shows a high level of colinearity. The observed colinearity made it possible to compare the order of SP-11, SRK, and SLG genes in the S locus between the five sequenced S haplotypes. It emerged that the order of SRK and SLG in class-II S-60is the reverse of that in the four class-I S haplotypes reported so far, and the order of SP11, SRK and SLG is the opposite of that in the class-I haplotype S-910. The possible gene designated as SAN1 ( S locus Anther-expressed Non-coding RNA like-1), which is located in the region between SP11-60 and SRK-60, has features reminiscent of genes for non-coding RNAs (ncRNAs), but no homologous sequences were found in the databases. This sequence is transcribed in anthers but not in stigmas or leaves. These features of the genomic structure of S-60 are discussed with special reference to the characteristics of class-II S haplotypes.SPRINGER-VERLAG, Jun. 2003, MOLECULAR GENETICS AND GENOMICS, 269(3) (3), 361 - 369, English[Refereed]Scientific journal
- Commonality of self-recognition specificity of S haplotypes between Brassica oleracea and Brassica rapaWe have identified several interspecific pairs of S haplotypes having highly similar SRK and SP11/SCR sequences between Brassica oleracea and Brassica rapa. The recognition specificities of S haplotypes in these pairs were examined with three different methods. Stigmas of interspecific hybrids between an S-32 homozygote in B. oleracea and an S-60 homozygote in B. rapa, which were produced to avoid the interspecific incompatibility between the two species, showed incompatibility to the pollen of an S-8 homozygote in B. rapa and to the pollen of an S-15 homozygote in B. oleracea, while it showed compatibility to the pollen of other S haplotypes, suggesting B. oleracea S-32 and B. rapa S-60 have the same recognition specificity as B. rapa S-8 and B. oleracea S-15. Pollen grains of transgenic S-60 homozygous plants in B. rapa carrying a transgene of SP11-24 from B. oleracea were incompatible to B. rapa S-36 stigma, indicating that B. oleracea S-24 and B. rapa S-36 have the same recognition specificity. Application of the SP11 protein of B. rapa S-41 and S-47 onto the surface of B. oleracea S-64 stigmas and S-12 stigmas, respectively, resulted in the incompatibility reaction to pollen grains of another S haplotype, but application onto the stigmas of other S haplotypes did not, suggesting that B. oleracea S-64 stigmas and S-12 stigmas recognized the B. rapa SP11-41 and SP11-47 proteins as self SP11 proteins, respectively. Besides having evolutionary implications, finding of many interspecific pairs of S haplotypes can provide insight into the molecular mechanism of self-recognition. Comparing deduced amino-acid sequences of SP11 proteins and SRK proteins in the pairs, regions of SP11 and SRK important for self-recognition are discussed.KLUWER ACADEMIC PUBL, Jun. 2003, PLANT MOLECULAR BIOLOGY, 52(3) (3), 617 - 626, English[Refereed]Scientific journal
- A self-incompatibility system is used for F(1) hybrid breeding in Brassicaceae vegetables. The determinants of recognition specificity of self-incompatibility in Brassica are SRK in the stigma and SP11/SCR in the pollen. Nucleotide sequences of SP11 alleles are more highly variable than those of SRK. We analyzed the S haplotype specificity of SP11 DNA by Southern-blot analysis and dot-blot analysis using 16 S haplotypes in Brassica oleracea, and found that DNA fragments of a mature protein region of SP11 cDNA, SP11(m), of eight S haplotypes can detect only the SP11 alleles of the same S haplotypes. This specificity makes these methods useful for S haplotype identification. Therefore, we developed two methods of dot-blot analysis for SP11. One is dot blotting of DNA samples, i.e. plant genomic DNA probed with labeled SP11(m), and the other is dot blotting of SP11(m) DNA fragments probed with labeled DNA samples, i.e. the SP11 coding region labeled by PCR using a template of plant genomic DNA. The former is useful for testing many plant materials. The latter is suitable, if there is no previous information on the S haplotypes of plant materials.SPRINGER, May 2003, THEORETICAL AND APPLIED GENETICS, 106(8) (8), 1433 - 1437, English[Refereed]Scientific journal
- Recognition specificity of self-incompatibility maintained after the divergence of Brassica oleracea and Brassica rapaThe determinants of recognition specificity of self-incompatibility in Brassica are SRK in the stigma and SP11/SCR in the pollen, respectively, In the pair of S haplotypes BrS46 (S-46 in B. rapa) and BoS(7) (S-7 in B. oleracea), which have highly similar SRK alleles, the SP11 alleles were found to be similar, with 96.1% identity in the deduced amino acid sequence. Two other pairs of S haplotypes, BrS47 and BoS(12), and BrS8 and BoS(32), having highly similar SRK and SP11 alleles between the two species were also found. The haplotypes in each pair are considered to have been derived from a single S haplotype in the ancestral species. The allotetraploid produced by interspecific hybridization between homozygotes of BrS46 and BoS(15) showed incompatibility with a BoS(7) homozygote and compatibility with other B. oleracea S haplotypes in reciprocal crossings. This result indicates that BrS46 and BoS(7) have maintained the same recognition specificity after the divergence of the two species and that amino acid substitutions found in such cases in both SRK alleles and SP11 alleles do not after the recognition specificity. DNA blot analysis of SRK, SP11, SLG and other S-locus genes showed different DNA fragment sizes between the interspecific pairs of S haplotypes. A much lower level of sequence similarity was observed outside the genes of SRK and SP11 between BrS46 and BoS(7). These results suggest that the DNA sequences of the regions intervening between the S-locus genes were diversified after or at the time of speciation. This is the first report demonstrating the presence of common S haplotypes in different plant species and presenting definite evidence of the trans-specific evolution of self-incompatibility genes.BLACKWELL PUBLISHING LTD, Jan. 2002, PLANT JOURNAL, 29(2) (2), 215 - 223, English[Refereed]Scientific journal
- 2024, 育種学研究, 26Unveiling the NB-LRR diversity from cultivars of Brassica rapa vegetables
- 2024, 園芸学研究 別冊, 23(1) (1)Brassica rapaの極晩抽性系統の選抜とFLCの春化応答性の調査
- 2023, 育種学研究, 25Refinement of white rust resistance gene loci in Brassica vegetables
- 2021, 園芸学研究 別冊, 20(1) (1)Development of a DNA marker for white rust resistance in Brassica vegetables.
- 2021, 育種学研究, 23Identification of a resistance gene to white rust in Brassica rapa L.
- 2021, 育種学研究, 23Fusarium resistance and FocBr1 polymorphism in Brassica rapa cultivars
- 2021, 日本植物病理学会大会プログラム・講演要旨予稿集, 2021Resistance mechanism to white rust (Albugo candida) in Brassica rapa L.
- 2021, 園芸学研究 別冊, 20(1) (1)Evaluation of the low temperature requirement for vernalization and growth stage responding to vernalization in Turnip rape (Brassica napus L.)
- 2020, 日本植物病理学会大会プログラム・講演要旨予稿集, 2020Genetic inheritance of white rust disease resistance and genome structure covering white rust resistance gene in Brassica rapa L.
- 2020, 育種学研究, 22Elucidation of genetic inheritance of white rust disease resistance and analysis of the genome structure covering white rust resistance gene in Brassica rapa L.
- 2020, 育種学研究, 22Fine mapping of QTL for tuberous stem formation of kohlrabi
- 06 Sep. 2019, 育種学研究, 21, 52, Japaneseアブラナ科におけるFLCパラログ間の発現量比について
- 2019, 園芸学研究 別冊, 18(1) (1)Brassica rapa L.における萎黄病・根こぶ病抵抗性DNAマーカー選抜系の応用
- 2019, 日本植物病理学会大会プログラム・講演要旨予稿集, 2019萎黄病抵抗性・感受性ハクサイ系統における萎黄病菌感染時の免疫応答
- Sep. 2018, 育種学研究, 20(2) (2), 212, Japaneseキャベツにおける葉齢依存的な春化応答性のトランスクリプトーム解析
- 24 Mar. 2018, 園芸学研究 別冊, 17(1) (1), 131, Japanese結球野菜の開花調節機構に関する研究の現状と今後の展望について
- 24 Mar. 2018, 園芸学研究 別冊, 17(1) (1), 132, Japaneseキャベツの緑体春化におけるFLOWERING LOCUS Cの役割
- 2018, 園芸学研究 別冊, 17(1) (1)ハクサイ市販品種‘W77’の両親系統間リシークエンスによる片親特異的遺伝子変異の同定
- 2018, 園芸学研究 別冊, 17(1) (1)萎黄病菌キャベツ分離菌抵抗性遺伝子DNAマーカーで萎黄病菌コマツナ分離菌抵抗性を予想できるか?
- 2018, 園芸学研究 別冊, 17(1) (1)ハクサイ市販品種‘W77’の両親系統のリシークエンスによるゲノム構造の系統間比較
- 2018, 育種学研究, 20ハクサイ初期生育期における子葉および本葉サイズ関連遺伝子の探索
- 2018, 育種学研究, 20コールラビ茎肥大性のトランスクリプトーム解析
- Oct. 2017, 育種学研究, 19(2) (2), 123, JapaneseBrassica oleracea FLC2領域を移入交雑で導入したBrassica rapaの特性解析
- Oct. 2017, アグリバイオ, 1(11) (11), 43 - 44, Japaneseアブラナ科植物の雑種強勢に関する研究[Invited]Introduction commerce magazine
- 12 Apr. 2017, 日本植物病理学会大会プログラム・講演要旨予稿集, 2017, 64, JapaneseBrassica rapa L.におけるアブラナ科植物白さび病抵抗性遺伝子の探索と検出
- 2017, 育種学研究, 19トランスクリプトーム解析を用いたハクサイの萎黄病菌感染時における免疫応答調査
- 2017, 育種学研究, 19シロイヌナズナの早期開花エピ変異体の形質評価
- 2017, 育種学研究, 19Brassica oleraceaにおける萎黄病抵抗性遺伝子FocBo1の多型解析と選抜マーカー開発
- 2017, 園芸学研究 別冊, 16(2) (2)萎黄病抵抗性・感受性ハクサイ系統における萎黄病菌感染時転写応答の解明
- 24 Sep. 2016, 育種学研究, 18, 115, Japaneseアブラナ科植物白さび病菌Albugo candidaに対する抵抗性遺伝子同定に向けたBrassica rapa抵抗性品種の選抜
- 2016, 園芸学研究 別冊, 15(2) (2)Brassica rapa L.における春化によるFLOWERING LOCUS Cのエピジェネティックな転写制御について
- 2016, 園芸学研究 別冊, 15(2) (2)ハクサイにおける萎黄病菌感染時のトランスクリプトーム解析による病害反応遺伝子の調査
- 2016, 園芸学研究 別冊, 15(2) (2)Whole genome bisulfite sequencingによるハクサイのDNAメチル化領域の探索
- 2016, 育種学研究, 18ハクサイにおけるメチローム解析
- 2016, 育種学研究, 18萎黄病菌を接種したときの抵抗性と感受性ハクサイ系統のトランスクリプトームプロファイリング
- GENETICS SOC JAPAN, Dec. 2015, GENES & GENETIC SYSTEMS, 90(6) (6), 373 - 373, EnglishFor the identification of the molecular mechanism of heterosis by epigenetic approach in BrassicaSummary international conference
- 26 Sep. 2014, 育種学研究, 16, 111, Japaneseハクサイ市販一代雑種品種“W77”における雑種強勢に関わるQTLの探索
- Transcriptional regulation of key gene of vernalization, FLOWERING LOCUS CTo adjust the timing of flowering, plants can sense the temperature and day length. Vernalization is a phenomenon where flowering is induced by a long period of low temperature, and has an important role in controlling flowering time. In Arabidopsis thaliana, FLOWERING LOCUS C (FLC) acts as a floral repressor, and transcription of FLC is repressed through the increase of tri-methylation of histone H3 Lysine 27 (H3K27me3) by vernalization. This transcriptional repression is caused by POLYCOMB REPRESSIVE COMPLEX 2 (PRC2) that functions to modify the H3K27me3. Two types of long non-coding RNAs (lncRNA) from the intragenic region of FLC, COOLAIR and COLDAIR, are expressed by vernalization. Notably COLDAIR leads to an increase of H3K27me3 with PRC2 interaction. A detailed understanding of the regulatory mechanism of the FLC will provide a good model of other epigenetically regulated genes.植物は温度や日長を認識することで開花するタイミングを調節している。春化は、植物が長期間の低温に遭遇することにより花成が誘導される現象であり、開花期を制御する重要な役割を担っている。シロイヌナズナ（Arabidopsis thaliana）の開花抑制遺伝子であるFLOWEING LOCUS C(FLC) では、春化によって、転写抑制にかかわるヒストンマークであるヒストンH3の27番目のリジン残基のトリメチル化（H3K27me3）を介した転写抑制が生じる。この遺伝子サイレンシングにはH3K27me3をターゲットとするPOLYCOMB RPRESSIVE COMPLEX 2（PRC2）複合体が関与する。これは動植物で保存された転写制御メカニズムである。FLC 遺伝子座内から転写される長鎖非翻訳RNA（long non-coding RNA: lncRNA）であるCOOLAIR とCOLDAIR は、春化によって誘導される。特に、COLDAIR はPRC2と結合することで、FLC 遺伝子座内のH3K27me3レベルの増加をもたらす。FLC の制御機構が詳細に理解されれば、他のエピジェネティックな制御を受ける遺伝子のモデルになるだろう。新潟大学農学部, Mar. 2014, 新潟大学農学部研究報告, 66(2) (2), 105 - 110, English
- Screening of DNA markers suitable for purity test of inbred lines in Brassica rapa親候補となる近交系統の作成は、植物の一代雑種(F1)品種の育成過程の最初の段階である。一般的に遺伝的に均一な近交系統の育成には5、6世代以上の自殖が必要であると考えられている。DNAマーカーを利用した近交系の純度検定は信頼性が高いが、Brassica rapa L. では、純度検定に有効なDNAマーカーの報告は少ない。本研究では、F1品種の両親系統間の多型を見分けることができるDNAマーカーを見出すために、321のSSRプライマー対を試した。F2集団では、両親系統のゲノムが分離することから、両親系統の塩基配列の多型を追跡することが可能となる。材料にはハクサイの市販F1品種であるW77のF2個体を用いた。59のDNAマーカーでF2集団の個体間で多型が見られたことから、これらのDNAマーカーは純度検定に有効的であると考えられた。新潟大学農学部, Mar. 2014, 新潟大学農学部研究報告, 66(2) (2), 111 - 124, English
- 2014, 園芸学研究 別冊, 13(1) (1)Differential expression解析を用いたハクサイ萎黄病抵抗性候補遺伝子の同定
- 2014, 育種学研究, 16ハクサイ市販一代雑種品種W77及びそのF2後代の形質調査
- 2014, 育種学研究, 16Brassica oleraceaにおける近交系の純度検定に最適なDNAマーカーのスクリーニングと汎用性
- 2014, 園芸学研究 別冊, 13(2) (2)Brassica oleracea近交系の遺伝的均一性評価およびF1種子純度検定に適したDNAマーカーの探索
- 2014, 育種学研究, 16DNAマーカーを用いたBrassica rapa近交系の基盤整備
- 2014, 育種学研究, 16アブラナ科萎黄病菌(Fusarium oxysporum)のde novo全ゲノムシークエンス情報を用いた分化型特異的遺伝子領域の探索
- 公益社団法人 日本農芸化学会, 01 May 2013, 化学と生物, 51(5) (5), 283 - 285, Japanese
- Screening of DNA markers suitable for purity test of inbred lines in Brassica oleracea近交系統の作成は、植物のF1品種の育成過程の最初の段階である。遺伝的に均一な近交系統の育成には5、6世代以上の自殖が必要であると考えられている。DNAマーカーを利用した近交系の純度検定は信頼性が高いが、B. oleraceaでは、純度検定に有効なDNAマーカーの報告は少ない。本研究では、F1品種の両親系統間の多型を見分けることができるDNA マーカーを見出すために、175のSSRプライマー対を試した。F2集団では、両親系統のゲノムが分離することから、両親系統の塩基配列の多型を追跡することが可能となる。そこで、材料にはキャベツの市販F1品種であるYR錦秋協力152のF2個体を用いた。12のDNAマーカーでF2集団の個体間で多型が見られたことから、これらのマーカーは純度検定に有効的であると考えられた。新潟大学農学部, Mar. 2013, 新潟大学農学部研究報告 = Bulletin of the Faculty of Agriculture, Niigata University, 65(2) (2), 137 - 147, English
- 2013, 園芸学研究 別冊, 12(2) (2)ハクサイF1品種W39における生育初期及び収穫期の雑種強勢の評価
- 2013, 園芸学研究 別冊, 12(2) (2)ハクサイF1品種「W77」における生育初期及び収穫期の雑種強勢の評価
- 2013, 育種学研究, 15Brassica oleracea染色体添加型B.rapaを用いた緑植物体春化の遺伝解析
- 2013, 育種学研究, 15Brassica oleracea品種における萎黄病抵抗性遺伝子FocBo1連鎖DNAマーカーと抵抗性との関連性
- Analysis of the promoter activity of anther-specific genes in Arabidopsis thaliana葯特異的遺伝子やそれらのプロモーター配列の解析は、葯発達に関わる遺伝子の同定や、遺伝子組換えによる雄性不稔植物の作出を目的として、これまで多くなされてきている。本研究では、6つのプロモーター配列の単離を行い、GUS遺伝子に連結してシロイヌナズナに導入することで、葯の発達段階における発現誘導様式を調べた。プロモーターGUSの発現は、様々な花粉発達ステージにおいて観察された。また、塩基配列の解析では葯/花粉特異的遺伝子の発現に必要とされるシス配列が多く含まれていたことから、これらのシス配列が葯特異的に発現誘導していることが示唆された。新潟大学農学部, Sep. 2012, 新潟大学農学部研究報告 = Bulletin of the Faculty of Agriculture, Niigata University, 65(1) (1), 31 - 37, English
- 植物の細胞質雄性不稔性と核遺伝子による稔性の制御について細胞質雄性不稔性(Cytoplasmic male sterility:CMS)は、ミトコンドリアゲノム上に存在する特有のorfによって引き起こされ、母性遺伝する形質である。多くの場合、核ゲノムにコードされる稔性回復遺伝子(Restorer of fertility:Rf)の存在下で、稔性が回復する。CMSとCMSの維持系統、Rf遺伝子を保持する稔性回復系統を組合せて利用する事で一代雑種育種を行う事ができるため、CMSやその稔性回復は農業上重要な現象である。これまでに同定されているRf遺伝子の多くは、ミトコンドリアに局在するpentatricopeptide repeat(PPR)タンパク質をコードしており、CMS関連ミトコンドリア遺伝子の発現を抑制している。新潟大学農学部, Mar. 2012, 新潟大学農学部研究報告 = Bulletin of the Faculty of Agriculture, Niigata University, 64(2) (2), 135 - 142, Japanese
- 白菜のSハプロタイプ間における自家不和合性の強度の比較アブラナ科野菜の自家不和合性の強度は、F1種子の生産において、自殖種子の混入を避けるために重要な要素である。アブラナ科植物の自家不和合性は、1つの複合遺伝子座(S複合座)によって胞子体型に制御されており、Sハプロタイプ間で優劣性が見られる。しかし、自家不和合性の強度と優劣性との関連性はあまり研究されていない。本研究では、Brassica rapa(白菜)のF1品種に由来するSホモ個体を作成し、3つの独立なSハプロタイプの組合せについて自家不和合性の強度を比較した。優劣性の有無にかかわらず、2つのclass IのSハプロタイプ間では自家不和合性の強度に違いが見られなかった。しかし、花粉において優劣性があるclass Iとclass IIのSハプロタイプ間では、class II Sハプロタイプの自家不和合性の強度はclass I Sハプロタイプに比べて弱かった。これらの結果から、Sハプロタイプ間における自家不和合性の強度は優劣性とは独立していることが示唆された。新潟大学農学部, Mar. 2012, 新潟大学農学部研究報告 = Bulletin of the Faculty of Agriculture, Niigata University, 64(2) (2), 143 - 149, English
- 2012, 育種学研究, 14Brassica oleracea根こぶ病抵抗性遺伝子の集積による抵抗性育種
- 2012, 育種学研究, 14キャベツ萎黄病抵抗性遺伝子Foc-Bo1の同定とファインマッピング
- 2012, 育種学研究, 14RNA-sequence法を用いたハクサイ品種間(S11×R09)特異的なNBS-LRRモチーフ遺伝子の探索
- Analysis of Organ-specific Regulation of DNA Methylation in Brassica rapa by MSAPDNA methylation is an important epigenetic modification regulating gene expression. In this study, we investigateddifferences of DNA methylation status between leaves, stamens, and pistils by methylation sensitive amplificationpolymorphism (MSAP) analysis in Brassica rapa. The difference of DNA methylation status was the largest between leavesand stamens/pistils. About half of the differentially methylated sequences were genic regions, but their expression levelsdid not differ between organs. The percentage of genic regions detected by MSAP analysis was higher than that of the totallength of the genic regions in the genome, suggesting that genic regions are differentially methylated between organs in B.rapa.DNA のメチル化は遺伝子の転写制御に重要なエピジェネティックな変化である。本研究では、Brassica rapa の葉、雄しべ、雌しべのDNA のメチル化レベルの違いについてMSAP 法を用いて調べた。DNA のメチル化は葉と雄しべ/ 雌しべで最も違っていた。DNA のメチル化が異なっていた配列の半分は遺伝子領域であったが、遺伝子の発現量は3つの器官で違いが見られなかった。MSAP 法で見出された領域の内、遺伝子領域が占める割合は、ゲノム中に占める遺伝子領域の割合よりも高かったことから、B. rapa において、器官特異的なDNA のメチル化の違いは遺伝子領域に起こりやすい可能性が示された。新潟大学農学部, Sep. 2011, 新潟大学農学部研究報告, 64(1) (1), 7 - 16, English
- Dominant/Recessive Relationship in Male Determinant Gene of Self-incompatibility in Brassicaアブラナ科植物の自家不和合性は、S複合座に座乗するSRK（S receptor kinase）とSP11／SCR（S locus protein 11／S locus cysteine rich）のS特異的な相互作用により決定されている。アブラナ科植物の自家不和合性は、胞子体型自家不和合性であることから、花粉の表現型は親の遺伝子型によって決定される。SP11／SCRの対立遺伝子間で優劣性が見られる場合、ヘテロ接合体の花粉では、劣性側のSP11／SCRの発現が抑制される。この劣性側のSP11／SCRの転写抑制は、優勢側のS複合座から、劣性側のSP11／SCRのプロモーター領域と相同性があるsmall RNAが発現し、その結果、劣性側のSP11／SCRのプロモーター領域にDNAのメチル化が誘導されることで起こる可能性が示唆された。新潟大学農学部, Sep. 2011, 新潟大学農学部研究報告, 64(1) (1), 17 - 26, Japanese
- エピジェネティクスが制御する植物の形質遺伝子の発現情報の伝達は塩基配列に依存するジェネティックな遺伝と塩基配列によらず、遺伝子の修飾状態の変化に依存するエピジェネティックな遺伝が存在する．植物のエピジェネティクス研究は、モデル植物であるシロイヌナズナで進んでおり、特に突然変異体を用いた解析がこの分野の進展に大きく貢献している．動物のエピジェネティックな制御に関連する遺伝子の突然変異体は、致死に到ることが多いが、植物の突然変異体では致死に到らず、遺伝学的な解析が可能であることが理由として挙げられる．また、エピジェネティックな遺伝子の発現制御は、遺伝子機能・環境応答・生殖制御などとの関連性が示唆されていることからも育種において重要な研究課題である．本総説では、育種学的観点からエピジェネティックな制御機構の重要性について、トランスポゾン・生殖・環境応答の項目に分けて概説し、またエピジェネティックな現象による重要農業形質の例について最近の話題を交えて紹介する．日本育種学会, 2010, 育種学研究, 12(4) (4), 123 - 131, Japanese[Refereed]Introduction scientific journal
- Recent molecular biological studies on Brassica self-incompatibility and future perspectivesアブラナ科植物の多くは、近親交配を防ぐために、自己花粉の発芽・柱頭内への花粉管の侵入を特異的に阻害する機構である自家不和合性を有している。アブラナ科植物の自家不和合性は、基本的には多数の複対立遺伝子を持つ1遺伝子によって説明され、花粉と雌しべのS対立遺伝子が一致したときに、柱頭内への花粉管の侵入が阻害される。また、花粉の認識特異性が胞子体の遺伝子型によって決定されるため、胞子体型自家不和合性に分類される。自己花粉が認識されて花粉管の侵入が柱頭上で抑制されることから、柱頭と花粉に自家不和合性の自他認識反応に関与する分子が存在すると考えられた。アブラナ科植物には、主要な野菜であるキャベツ、ブロッコリー、カリフラワーやカブ、ハクサイ、ツケナ類、チンゲンサイ及び、ダイコンが含まれ、自家不和合性を利用した一代雑種品種の採種が商業的に行われている。アブラナ科植物の自家不和合性は、分子生物学的に興味深い現象であると同時に、育種学的にも重要な研究課題である。Japanese Society of Breeding, Mar. 2008, Breeding Research, 10(1) (1), 1 - 9, JapaneseIntroduction other
- GENETICS SOC JAPAN, Dec. 2007, GENES & GENETIC SYSTEMS, 82(6) (6), 549 - 549, EnglishEvolution of imprinted FWA gene in ArabidopsisSummary international conference
- 26 Aug. 2002, 育種学研究 = Breeding research, 4, 346 - 346, JapaneseAnalysis of genome structure of class II self-incompatibility (s) locus in Brassica rapa
- 26 Aug. 2002, 育種学研究 = Breeding research, 4, 347 - 347, JapaneseComparison of genome structure of class I self-incompatibility (S) locus between Brassica oleracea and Brassica rapa
- 30 Mar. 2002, 育種学研究 = Breeding research, 4(1) (1), 256 - 256, JapaneseIdentification of S haplotype in Brassica by dot blot analysis of SP11 alleles
- Contributor, Chapter 1, Springer Singapore, Jan. 2023, ISBN: 9789811953668The role of epigenetic transcriptional regulation in Brassica vegetables: a potential resource for epigenetic breeding
- Joint work, 第96巻・第12号 1075-1085, YOKENDO, Dec. 2021The current status and perspective of research on hybrid vigor in plants
- Joint work, pp 59-94, Advances in Plant Breeding Strategies: Vegetable Crops. Springer, Cham, Aug. 2021Chinese Cabbage (Brassica rapa L. var. pekinensis) Breeding: Application of Molecular Technology
- Joint work, IntechOpen, Feb. 2021Breeding for Disease Resistance in Brassica Vegetables Using DNA Marker Selection
- Joint work, 161-256, Springer, Mar. 2020The Importance of Genetic and Epigenetic Research in the Brassica Vegetables in the Face of Climate Change
- Joint work, Advances in Botanical Research-Plant Epigenetics Coming of Age for Breeding Applications, Nov. 2018, EnglishChapter 8 Hybrid Vigor: Importance of Epigenetic Processes and Consequences for BreedingScholarly book
- Joint work, IntechOpen, Oct. 2018, EnglishGenetic and Epigenetic Regulation of Vernalization in BrassicaceaeScholarly book
- Others, 朝倉書店, Sep. 2018, Japanese遺伝学の基礎 14章 エピジェネティクスTextbook
- Joint work, 日本育種学会, Jul. 2014, Japanese育種学研究Scholarly book
- Joint work, 日本農芸化学会, May 2013, Japanese植物の雑種強勢の分子生物学的な研究と展望Scholarly book
- Joint work, Advances in Botanical Research, 2007, EnglishSelf‐IncompatibilityScholarly book
- Plant and Animal Genome Conference (PAG32), Jan. 2025, EnglishIdentification of Factors That Regulate Early Flowering in ddm1 Mutant of C24 Accession in Arabidopsis thalianaPoster presentation
- Plant and Animal Genome Conference (PAG32), Jan. 2025, EnglishCommon Mechanisms Between the Decrease in Heterosis Levels By Loss of DDM1 Function and the Reduction in Biomass By Shade Avoidance SyndromePoster presentation
- 令和6年度野菜花き課題別研究会-アブラナ科野菜の育種・生産を取り巻く現状と展望, Oct. 2024, JapaneseDNA マーカー選抜による複合病害抵抗性育種[Invited]Invited oral presentation
- Special Seminar-University of Nebraska–Lincoln, Sep. 2024, EnglishGenetic and epigenetic approaches to hybrid vigor, vernalization, ad disease responsiveness in BrassicaceaePublic discourse
- 日本育種学会 第146回講演会, Sep. 2024, JapaneseBrassica rapa野菜 NB-LRR遺伝子の掌握Poster presentation
- 日本育種学会 第146回講演会, Sep. 2024, JapaneseシロイヌナズナC24 系統のddm1変異体を早期開花させる因子の探索Oral presentation
- 日本育種学会第146回講演会, Sep. 2024, Japanese老化関連遺伝子が DDM1の機能喪失による雑種強勢低減と避陰反応による葉面積減少に関与する可能性についてOral presentation
- 令和６年度園芸学会近畿支部滋賀大会, Aug. 2024, Japaneseハクサイにおける避陰反応による生育低下を引き起こす原因遺伝子の探索Oral presentation
- 令和６年度園芸学会近畿支部滋賀大会, Aug. 2024, JapaneseBrassica rapaにおける根こぶ病菌感染により発現が変動する遺伝子の同定Poster presentation
- The 13th International Congress on Plant Molecular Biology, Jun. 2024, EnglishFunctional assessment and epigenome editing of Brassica rapa FLC in Arabidopsis thalianaPoster presentation
- The 13th International Congress on Plant Molecular Biology, Jun. 2024, EnglishAre additive histone modifications important for exhibiting heterosis in Chinese cabbage?Poster presentation
- International Conference on Advanced Agricultural Research, May 2024, EnglishIDENTIFICATION OF GENES WHOSE EXPRESSION FLUCTUATES FOLLOWING PATHOGEN INFECTION IN BRASSICA RAPA VEGETABLES[Invited]Invited oral presentation
- 園芸学会令和6年度春季大会, Mar. 2024ナデシコ属植物の春化応答に関わるFLCオルソログの解析Poster presentation
- 園芸学会令和6年度春季大会, Mar. 2024Brassica rapaの晩抽性分子育種への試みOral presentation
- 日本育種学会 第145回講演会, Mar. 2024, Japaneseシロイヌナズナのddm1変異体の系統間比較Oral presentation
- The 16th International Rapeseed Congress (IRC) 2023, Sep. 2023, EnglishComparison of the transcriptional response of FLC following vernalization in two different growth stages in artificially synthesized Brassica napusPoster presentation
- International Plant and Animal Genome Conference (PAG Australia), Sep. 2023, EnglishLoss of DDM1 function leads to reduced levels of heterosis in Arabidopsis thalianaPoster presentation
- International Plant and Animal Genome Conference (PAG Australia), Sep. 2023, EnglishCharacterization of FLOWERING LOCUS C 5 in Brassica rapa L.Poster presentation
- International Plant and Animal Genome Conference (PAG Australia), Sep. 2023, EnglishIdenfification of the genetic region related to heterosis in Arabidopsis thalianaPoster presentation
- International Plant and Animal Genome Conference (PAG Australia), Sep. 2023, EnglishCharacterization of the transcriptional immune response to Albugo candida in Brassica rapa L.Poster presentation
- International Plant and Animal Genome Conference (PAG Australia), Sep. 2023, EnglishOverexpression of ASC8 affects growth and heterosis in Arabidopsis thalianaPoster presentation
- International Plant and Animal Genome Conference (PAG Australia), Sep. 2023, EnglishThe Association between Salt Tolerance and Enhancement of Biomass in Arabidopsis thaliana HybridsPoster presentation
- 日本育種学会 第144回講演会, Sep. 2023, Japaneseコマツナにおける白さび病菌感染による転写応答の系統間比較Poster presentation
- 日本育種学会 第144回講演会, Sep. 2023, Japaneseシロイヌナズナの耐塩性とバイオマスのヘテロシ スの関係性Oral presentation
- 日本育種学会 第144回講演会, Sep. 2023, Japaneseシロイヌナズナの雑種強勢に関わるゲノム領域の探求Oral presentation
- 日本育種学会 第144回講演会, Sep. 2023, JapaneseDDM1の機能喪失による雑種強勢低減と避陰反応による生育低下に共通する遺伝子の探索Oral presentation
- 日本育種学会 第144回講演会, Sep. 2023, Japaneseアブラナ科野菜における白さび病抵抗性遺伝子座 乗領域の詳細化Oral presentation
- 日本育種学会 第144回講演会, Sep. 2023, Japaneseシロイヌナズナにおける ACS8 の発現上昇が雑種 強勢に及ぼす影響Oral presentation
- 令和５年度 園芸学会近畿支部兵庫大会, Sep. 2023, JapaneseBrassica rapaの極晩抽性系統の選抜とFLCの春化応答性の調査Oral presentation
- 令和５年度 園芸学会近畿支部兵庫大会, Sep. 2023, JapaneseBrassica rapaにおけるFLOWERING LOCUS C 5の評価Oral presentation
- 令和５年度 園芸学会近畿支部兵庫大会, Sep. 2023, JapaneseBrassica rapaエピジェネティック変異体におけるDNAメチル化レベルの低下と生育への影響Oral presentation
- The 4th Asian Horticultural Congress (AHC2023), Aug. 2023, EnglishRole of FLOWERING LOCUS C 5 as a floral repressor in Brassica rapa L.Poster presentation
- The 4th Asian Horticultural Congress (AHC2023), Aug. 2023, EnglishComparison of the histone modification H3 lysine 27 tri-methylation states between accessions in Chinese cabbagePoster presentation
- The 4th Asian Horticultural Congress (AHC2023), Aug. 2023, EnglishTyping and distribution of the SFBB alleles encoding in four S-haplotypes of European pear (Pyrus communis L.)Poster presentation
- The 4th Asian Horticultural Congress (AHC2023), Aug. 2023, EnglishIdentification of canididate genes associated with the defense response following Albugo candida inoculation in Brassica rapa L.Oral presentation
- The 4th Asian Horticultural Congress (AHC2023), Aug. 2023, EnglishGenetic and epigenetic approaches for understanding molecular mechanism of heterosis in shoots at early developmental stages of Chinese cabbageOral presentation
- The 4th Asian Horticultural Congress (AHC2023), Aug. 2023, EnglishNew Chinese cabbage cultivar 'Saiten Neo 70' reduces the risk of clubroot diseasePoster presentation
- The 4th Asian Horticultural Congress (AHC2023), Aug. 2023, EnglishAlbugo candida inoculation has different effects on DNA methylation in resistant and susceptible accessions of komatsunaPoster presentation
- The 33rd International Conference on Arabidopsis Research (ICAR2023), Jun. 2023, EnglishInvestigation of heterosis in the salinity tolerance of intraspecific hybrid in Arabidopsis thalianaPoster presentation
- The 33rd International Conference on Arabidopsis Research (ICAR2023), Jun. 2023, EnglishHigh parent dominance of trimethylation of lysine 27 of histone H3 level in intraspecific hybrids of Arabidopsis thalianaPoster presentation
- The 33rd International Conference on Arabidopsis Research (ICAR2023), Jun. 2023, EnglishFor identification of factors on early flowering in ddm1 mutant of C24 accession in Arabidopsis thalianaPoster presentation
- The 33rd International Conference on Arabidopsis Research (ICAR2023), Jun. 2023, EnglishThe Influence of loss of DECREASE IN DNA METHYLATION 1 function on heterosis in Arabidopsis thalianaPoster presentation
- 園芸学会令和5年度春季大会, Mar. 2023, JapaneseBrassica rapaにおけるH3K4me3とH3K36me3の遺伝子発現における役割Oral presentation
- 園芸学会令和5年度春季大会, Mar. 2023, EnglishInduction of salicylic acid-responsive genes triggers disease resistance of Brassica rapa L.Oral presentation
- 日本育種学会第143回講演会, Mar. 2023, Englishコールラビの塊茎肥大に関連するコールラビとブロッコリーの比較トランスクリプトームと植物ホルモンプロファイリングOral presentation
- 日本育種学会第143回講演会, Mar. 2023, Japaneseアブラナ科野菜の晩抽性育種においてBrFLC5は考慮しなくていいか?Oral presentation
- 筑波大学形質転換植物デザイン研究拠点令和4年度成果報告会, Mar. 2023, Japaneseシロイヌナズナの早期開花変異体の原因遺伝子同定に向けてOral presentation
- Cold Spring Harbor Asia conference on Integrative Epigenetics in Plants, Dec. 2022, EnglishGenetic and epigenetic approaches for understanding the molecular mechanism of heterosis in Arabidopsis thaliana and Brassica rapaOral presentation
- Cold Spring Harbor Asia conference on Integrative Epigenetics in Plants, Dec. 2022, EnglishChange of trimethylation of lysine 27 of histone H3 in the decrease in DNA methylation 1 mutant of Arabidopsis thalianaPoster presentation
- Cold Spring Harbor Asia conference on Integrative Epigenetics in Plants, Dec. 2022, EnglishThe relationship between the function of DECREASE IN DNA METHYLATION 1 (DDM1) and the salinity tolerance of Arabidopsis thaliana accessions, Columbia-0 and C24Poster presentation
- Cold Spring Harbor Asia conference on Integrative Epigenetics in Plants, Dec. 2022, EnglishIdentification of the genetic region related to heterosis in Arabidopsis thalianaPoster presentation
- Cold Spring Harbor Asia conference on Integrative Epigenetics in Plants, Dec. 2022, EnglishRole of four major histone midifications in regulating heterosis in Chinease cabbagePoster presentation
- Cold Spring Harbor Asia conference on Integrative Epigenetics in Plants, Dec. 2022, EnglishThe role of DNA methylation in disease resitance against Albugo candida inoculation in Brassica rapa var. perviridisPoster presentation
- 日本育種学会第142回講演会, Sep. 2022, Japaneseシロイヌナズナの雑種強勢に関係する遺伝領域の同定Oral presentation
- 日本育種学会第142回講演会, Sep. 2022, Japaneseシロイヌナズナの耐塩性における雑種強勢の評価Oral presentation
- 園芸学会令和4年度秋季大会, Sep. 2022, Japaneseハクサイにおける長鎖非コードRNAゲノム領域のエピジェネティック修飾Poster presentation
- 園芸学会令和4年度秋季大会, Sep. 2022, Japaneseハクサイにおいて活性型ヒストン修飾が遺伝子発現に及ぼす影響Poster presentation
- The 31st International Horticultural Congress (IHC2022), Aug. 2022, EnglishThe role of histone modification in gene expression in Brassica rapa vegetablesOral presentation
- The 31st International Horticultural Congress (IHC2022), Aug. 2022, EnglishThe role of salicylic acid responsive genes in disease resistance in Brassica rapa vegetablesOral presentation
- The 31st International Horticultural Congress (IHC2022), Aug. 2022, EnglishThe role of epigenetic modifications in the transcriptional regulation of long noncoding RNAs in Brassica rapa vegetablesOral presentation
- School of Life and Medical Sciences Research Conference, Jun. 2022, EnglishThe epigenome analysis in Brassica rapa L.[Invited]Invited oral presentation
- 園芸学会令和4年度春季大会, Mar. 2022, JapaneseBrassica rapaにおける白さび病菌の感染がDNAメチル化に及ぼす影響Poster presentation
- 日本育種学会第141回講演会, Mar. 2022, JapaneseBrassica rapaにおける極晩抽性系統の春化特性評価Oral presentation
- 園芸学会令和4年度春季大会, Mar. 2022, JapaneseニホンナシS2, S3, S4ハプロタイプのゲノム構造解析とSFBBの探索Oral presentation
- 園芸学会令和4年度春季大会, Mar. 2022, EnglishProspects for increasing the density of genetic markers in Chinese cabbage linkage map using GRAS-Di techniqueOral presentation
- 園芸学会令和4年度春季大会, Mar. 2022, EnglishTranscriptional association of mRNAs and their paired natural antisense transcripts following Fusarium oxysporum inoculation in Brassica rapa L.Oral presentation
- 筑波大学形質転換植物デザイン研究拠点 令和3年度成果報告会, Mar. 2022, JapaneseBrassica rapaの春化応答因子の同定に向けてOral presentation
- 2021 Cold Spring Harbor meeting: Plant Genomes, Systems Biology and Engineering (Virtual) ., Dec. 2021, EnglishComparative paralog histone modification states following whole genome triplication in Brassica rapa L.Poster presentation
- 園芸学会令和3年度秋季大会, Sep. 2021, Japaneseアブラナ科野菜における新規萎黄病抵抗性 DNA マーカーの開発Poster presentation
- 園芸学会令和3年度秋季大会, Sep. 2021, JapaneseBrassica rapa L. における萎黄病抵抗性育種のための共優性 SSR マーカーの開発Poster presentation
- 2021 Australian Brassica Conference, Sep. 2021, EnglishCharacterization of Histone H3 Lysine 4 and 36 Tri-methylation in Brassica rapa L.Oral presentation
- 園芸学会令和３年度春季大会, Mar. 2021, Japaneseアブラナ科野菜における白さび病抵抗性DNAマーカーの開発Poster presentation
- 園芸学会令和３年度春季大会, Mar. 2021, Japaneseアブラナ科野菜におけるサリチル酸応答遺伝子の同定Poster presentation
- 園芸学会令和３年度春季大会, Mar. 2021, Japaneseナバナ（Brassica napus L.）の春化に必要な低温要求量と春化応答を示す生育ステージの評価Poster presentation
- 日本育種学会 第139回講演会, Mar. 2021, JapaneseBrassica rapa品種の萎黄病抵抗性とFocBr1抵抗性遺伝子の多型解析
- 日本育種学会 第139回講演会, Mar. 2021, JapaneseBrassica rapa L.における白さび病抵抗性遺伝子の同定Oral presentation
- 令和3年度日本植物病理学会大会, Mar. 2021, JapaneseBrassica rapa L.における白さび病抵抗性機構についてOral presentation
- 日本育種学会 第138回講演会, Oct. 2020, JapaneseDNAメチル化転移酵素によるシロイヌナズナの雑種強勢制御機構Oral presentation
- 日本育種学会 第138回講演会, Oct. 2020, Japaneseシロイヌナズナにおけるヒストン脱アセチル化酵素HDA6が雑種強勢に及ぼす影響Oral presentation
- 日本育種学会第137回講演会, Mar. 2020キャベツにおける春化応答性の品種間差とBoFLC2遺伝子の関係Oral presentation
- 日本育種学会第137回講演会, Mar. 2020Brassica rapa L. における白さび病抵抗性遺伝子の遺伝様式の解明と抵抗性遺伝子候補の探索Oral presentation
- 日本育種学会第137回講演会, Mar. 2020キャベツ BoFLC2 導入ハクサイ系統の春化特性Poster presentation
- 日本育種学会第137回講演会, Mar. 2020コールラビ茎肥大性 QTL のファインマッピングPoster presentation
- Improvement of important agricultural traits based on genomic information of horticultural crops, Jan. 2020Transcriptome analysis before and after salicylic acid treatments in Brassica rapa L.Oral presentation
- Improvement of important agricultural traits based on genomic information of horticultural crops, Jan. 2020Comparison of the Transcriptome profile between F1 hybrid cultivar and it's parental lines at two early developmental stages in Chinese cabbage.Oral presentation
- Improvement of important agricultural traits based on genomic information of horticultural crops, Jan. 2020Characterization of FLOWERLING LOCUS C parlors in a late flowering line of Brassica rapa.Oral presentation
- Plant and Animal Genome XXVIII Conference, Jan. 2020, EnglishTranscriptome analysis before and after salicylic acid treatments in Brassica rapa L.Poster presentation
- 園芸学会令和元年度秋季大会, Sep. 2019春化によりキャベツ由来のFLC2がハクサイの遺伝的背景でどのように機能するかOral presentation
- 園芸学会令和元年度秋季大会, Sep. 2019Comparison of the Transcriptome profile between F1 hybrid cultivar and its parental lines at two early developmental stages in Chinese cabbageOral presentation
- 園芸学会令和元年度秋季大会, Sep. 2019Characterization of FLOWERING LOCUS C parlors in a late flowering line of Brassica rapaOral presentation
- 園芸学会令和元年度秋季大会, Sep. 2019ハクサイにおける長鎖非コードRNAとエピジェネティクスとの関係性Oral presentation
- 園芸学会令和元年度秋季大会, Sep. 2019ペチュニア花粉におけるニホンナシSFBB融合タンパク質発現系の開発Oral presentation
- 園芸学会令和元年度秋季大会, Sep. 2019ニホンナシSホモ系統の花粉RNAからのPsSFBB配列の再構築とクローニングOral presentation
- 日本育種学会第136回講演会, Sep. 2019トランスクリプトーム解析によるシロイヌナズナDDM1変異雑種における雑種強勢低減の改名についてOral presentation
- 日本育種学会第136回講演会, Sep. 2019アブラナ科におけるFLCパラログ間の発現量比についてOral presentation
- 2019 KSBB & SABRAO International Conference on Plant Breeding for Sustainable Development, Jul. 2019, English, Kimdaejung Convention Center, Gwangju, International conferenceVernalization alters histone H3 lysine 27 trimethylation at FLC locus in Brassica rapaPoster presentation
- 2019 KSBB & SABRAO International Conference on Plant Breeding for Sustainable Development, Jul. 2019, English, Kimdaejung Convention Center, Gwangju, International conferenceIdentification of non-additively expressed genes at early developmental stages in an F1 hybrid cultivar of Chinese cabbagePoster presentation
- International Symposium; Principles of pluripotent stem cells underlying plant vitality, May 2019, English, Tohoku University, International conferenceTranscriptome analysis indicate mechanisms underlying the DDM1-mediated heterosis in Arabidopsis thalianaPoster presentation
- International Symposium; Principles of pluripotent stem cells underlying plant vitality 英文, May 2019, English, Tohoku University, International conferenceCharacterization of the transcriptome in heterotic F1 hybrids in Arabidopsis thalianaPoster presentation
- 平成31年度日本植物病理学会大会, Mar. 2019, Japanese, つくば国際会議場, Domestic conference萎黄病抵抗性・感受性ハクサイ系統における萎黄病菌感染時の免疫応答Oral presentation
- 園芸学会平成３１年度春季大会, Mar. 2019, Japanese, 明治大学, Domestic conferenceハクサイにおける全ゲノムメチル化解析Oral presentation
- 日本育種学会第135回講演会, Mar. 2019, Japanese, 千葉大学, Domestic conferenceハクサイにおけるノンコーディングRNAの同定Oral presentation
- 園芸学会平成３１年度春季大会, Mar. 2019, English, 明治大学, Domestic conferenceDistribution of histone H3 lysine 27 trimethylation in different tissues of Brassica rapaOral presentation
- Improvement of important agricultural traits based on genomic information of horticultural crops, Dec. 2018, English, Sunchon National University, International conferenceMolecular and cellular characteristics of hybrid vigour in a commercial hybrid of Chinese cabbage.Public discourse
- Improvement of important agricultural traits based on genomic information of horticultural crops, Dec. 2018, English, Sunchon National University, International conferenceEpigenetics and heterosisPublic discourse
- 第13回東北育種研究集会, Nov. 2018, Japanese, Domestic conferenceBrassica oleraceaにおける異なる菌株に対する根こぶ病QTL解析Poster presentation
- 平成30年度野菜花き課題別研究会 「アブラナ科野菜における育種と栽培の現状と展望」, Oct. 2018, Japanese, 愛知県産業労働センター, Domestic conferenceアブラナ科野菜における雑種強勢研究の動向Oral presentation
- 日本育種学会第134回講演会, Sep. 2018, Japanese, Domestic conferenceハクサイ初期生育期における子葉および本葉サイズ関連遺伝子の探索Oral presentation
- 園芸学会平成30年度秋季大会, Sep. 2018, Japanese, 鹿児島大学, Domestic conferenceニホンナシSホモ系統における花柱内の和合・不和合花粉管伸長Oral presentation
- 平成30年度園芸学会近畿支部会大阪大会, Sep. 2018, Japanese, 大阪府立大学, Domestic conferenceニホンナシS3-RNase周辺領域のBACコンティグの拡張によるPpSFBB3の探索Poster presentation
- 日本育種学会第134回講演会, Sep. 2018, Japanese, Domestic conferenceコールラビ茎肥大性のトランスクリプトーム解析Poster presentation
- 日本育種学会第134回講演会, Sep. 2018, Japanese, Domestic conferenceキャベツにおける葉齢依存的な春化応答性のトランスクリプトーム解析Poster presentation
- 日本育種学会第134回講演会, Sep. 2018, Japanese, Domestic conferenceアブラナ科植物におけるPCR-RFLP法によるSハプロタイプ判定法が直面する問題点Oral presentation
- Seminar in AgriBio, Sep. 2018, English, La Trobe University, International conferenceThe molecular basis of vernalization in Brassica rapaPublic discourse
- AusCanola2018, Sep. 2018, English, Rendezvous Hotel Scarborough, International conferenceHow Brassica oleracea FLOWERING LOCUS C2 works in a Brassica rapa genetic backgroundPoster presentation
- AusCanola2018, Sep. 2018, English, Rendezvous Hotel Scarborough, International conferenceCharacterization of FLOWERING LOCUS C genes in leafy Brassica rapa vegetablesPoster presentation
- 日本育種学会第134回講演会, Sep. 2018, Japanese, Domestic conferenceBrassica rapaにおけるメチローム解析Poster presentation
- 平成30年度園芸学会近畿支部大阪大会, Sep. 2018, Japanese, 大阪府立大学 中百舌鳥キャンパス, Domestic conferenceBrassica rapa L.における萎黄病・根こぶ病抵抗性DNAマーカー選抜系の応用Oral presentation
- INTERNATIONAL PLANT MOLECULAR BIOLOGY 2018, Aug. 2018, English, International conferenceTranscriptome Difference by Fusarium inoculation between Fusarium yellows resistant and susceptible lines in Brassica rapa L.Poster presentation
- Brassica 2018, Jul. 2018, English, Saint-Malo, France, International conferenceQTL mapping combined with transcriptome analysis using broccoli and kohlrabiPoster presentation
- Brassica 2018, Jul. 2018, English, Saint-Malo, France, International conferenceCharacterization of epigenetic states in Brassica rapa L.Oral presentation
- The 25th International Congress on Sexual Plant Reproduction, Jun. 2018, English, Nagaragawa Convention Center, International conferenceIdentification of long non-coding RNAs before and after prolonged cold treatment in Brassica rapa L.Poster presentation
- The 25th International Congress on Sexual Plant Reproduction, Jun. 2018, English, Nagaragawa Convention Center, International conferenceDDM1 has an important function on heterosis in Arabidopsis thalianaPoster presentation
- The 25th International Congress on Sexual Plant Reproduction, Jun. 2018, English, Nagaragawa Convention Center, International conferenceCharacterization of histone H3 lysine 27 tri-methylation in Brassica rapa L.Poster presentation
- 園芸学会平成30年度春期大会, Mar. 2018, Japanese, 近畿大学農学部, Domestic conference結球野菜の開花調節機構に関する研究の現状と今後の展望についてPublic symposium
- 園芸学会平成30年度春期大会, Mar. 2018, Japanese, Domestic conference萎黄病菌キャベツ分離菌抵抗性遺伝子DNAマーカーで萎黄病菌コマツナ分離菌抵抗性を予測できるか?Poster presentation
- 園芸学会平成30年度春期大会, Mar. 2018, Japanese, Domestic conferenceハクサイ市販品種'W77'の両親系統間リシークエンスによる片親特異的遺伝子変異の同定Oral presentation
- 園芸学会平成30年度春期大会, Mar. 2018, Japanese, Domestic conferenceキャベツの緑体春化におけるFLOWERING LOCUS Cの役割Public symposium
- 日本育種学会第133回講演会, Mar. 2018, Japanese, 九州大学, Domestic conferenceLncRNA BrFLC2as in Brassica rapa differs from Arabidopsis thaliana COOLAIRPoster presentation
- 園芸学会平成30年度春期大会, Mar. 2018, Japanese, 近畿大学農学部, Domestic conferenceBrassica rapaにおける春化経路関連遺伝子の機能解析Oral presentation
- 第１２回東北育種研究集会, Nov. 2017, Japanese, 秋田県立大学, Domestic conference萎黄病抵抗性タンパク質と相互作用するタンパク質の同定に向けてPoster presentation
- 第１２回東北育種研究集会, Nov. 2017, Japanese, 秋田県立大学, Domestic conferenceBrassica rapaにおける萎黄病抵抗性遺伝子FocBr1の多型解析と選抜マーカーの開発Poster presentation
- 日本育種学会第132回講演会, Oct. 2017, English, Domestic conferenceRe-sequencing and SNP analysis of the parental lines of a commercial F1 hybrid cultivar of Chinese cabbagePoster presentation
- 日本育種学会第132回講演会, Oct. 2017, English, Domestic conferenceBrassica oleracea FLC2領域を移入交雑で導入したBrassica rapaの特性解析Oral presentation
- 園芸学会平成29年度秋期大会, Sep. 2017, Japanese, Domestic conference萎黄病抵抗性•感受性ハクサイ系統における萎黄病菌感染時転写応答の解明Oral presentation
- 平成29年度園芸学会近畿支部滋賀大会, Sep. 2017, Japanese, Domestic conferenceハクサイ市販品種'W77'の両親系統のリシークエンスによるゲノム構造の系統間比較Poster presentation
- 平成29年度園芸学会近畿支部滋賀大会, Sep. 2017, Japanese, ピアザ淡海, Domestic conferenceハクサイ市販品種'W77'における初期生育の雑種強勢関連領域の同定にむけてOral presentation
- 園芸学会平成29年度秋期大会, Sep. 2017, Japanese, Domestic conferenceニホンナシS2およびS4ハプロタイプ間のゲノム構造比較Oral presentation
- 園芸学会平成29年度秋期大会, Sep. 2017, Japanese, Domestic conferenceMeDIP-seqによるハクサイ系統間のDNAメチル化比較解析Oral presentation
- 園芸学会平成29年度秋期大会, Sep. 2017, Japanese, Domestic conferenceBrassica rapa L.における春化によるFLCのエピジェネティックな修飾変化の系統間比較Oral presentation
- THE PLANT EPIGENETICS CONSORTIUM IN JAPAN- SECOND MEETING, Aug. 2017, English, Domestic conferenceSimilar but different: the molecular basis of vernalization in Brassica rapaOral presentation
- Plant Biology 2017, Jun. 2017, English, International conferenceDDM1 is required for a full level of heterosis in Arabidopsis thalianaPoster presentation
- 医学生物学電子顕微鏡技術学会第33回学術講演会, May 2017, Japanese, Domestic conferenceニホンナシ柱頭の表面および微細構造の変化と柱 頭浸出液の出現についてOral presentation
- 平成29年度日本植物病理学会大会, Apr. 2017, Japanese, Domestic conferenceBrassica rapa L. におけるアブラナ科 植物白さび病抵抗性遺伝子の探索と検 出Oral presentation
- 日本育種学会第131回講演会, Mar. 2017, Japanese, 名古屋大学, Domestic conferenceトランスクリプトーム解析を用いたハクサイの萎黄病菌感染時における免疫応答調査Oral presentation
- 日本育種学会第131回講演会, Mar. 2017, Japanese, 名古屋, Domestic conferenceシロイヌナズナの早期開花エピ変異体の形質評価Oral presentation
- 日本育種学会第131回講演会, Mar. 2017, Japanese, 名古屋大学, Domestic conferenceアブラナ科植物におけるゲノム多様性および雑種強勢に関する研究[Invited]Invited oral presentation
- 日本育種学会第131回講演会, Mar. 2017, Japanese, 名古屋大学, Domestic conferenceBrassica oleraceaにおける萎黄病抵抗性遺伝子FocBo1の多型解析と選抜マーカー開発Poster presentation
- Brassica 2016, Oct. 2016, English, International conferenceThe epigenetic regulation of FLC expression by vernalization in Brassica rapa LPoster presentation
- Brassica 2016, Oct. 2016, English, International conferenceQTL mapping for stem swelling trait of Kohlrabi (Brassica oleracea var. gongylodes)Oral presentation
- Brassica 2016, Oct. 2016, English, International conferenceIdentification of the Fusarium yellows resistance genes; its application for marker-assisted selection in Brassica rapaOral presentation
- Brassica 2016, Oct. 2016, English, International conferenceGenome-wide analysis of DNA methylation in Chinese cabbagePoster presentation
- Brassica 2016, Oct. 2016, English, International conferenceCharacterization of early developmental and yield heterosis in Chinese cabbageOral presentation
- 日本育種学会第130回講演会, Sep. 2016, Japanese, 鳥取大学, Domestic conference萎黄病菌を接種したときの抵抗性と感受性ハクサイ系統のトランスクリプトームプロファイリングPoster presentation
- 園芸学会平成28年度秋季大会, Sep. 2016, Japanese, 名城大学, Domestic conferenceハクサイにおける萎黄病菌感染時のトランスクリプトーム解析による病害反応遺伝子の調査Oral presentation
- 日本育種学会第130回講演会, Sep. 2016, Japanese, Domestic conferenceハクサイにおけるメチローム解析Oral presentation
- 日本育種学会第130回講演会, Sep. 2016, Japanese, 鳥取大学, Domestic conferenceハクサイにおけるエピゲノム解析Public symposium
- 園芸学会平成28年度秋季大会, Sep. 2016, Japanese, 名城大学, Domestic conferenceニホンナシS3-RNase 周辺BACコンティグの塩基配列解析Oral presentation
- 園芸学会平成28年度秋季大会, Sep. 2016, Japanese, 名城大学, Domestic conferenceニホンナシS2遺伝子座のゲノム構造および発現遺伝子解析Oral presentation
- 日本育種学会第130回講演会, Sep. 2016, Japanese, Domestic conferenceシロイヌナズナの雑種強勢に関与する遺伝子領域の探索Oral presentation
- 日本育種学会第130回講演会, Sep. 2016, Japanese, 鳥取大学, Domestic conferenceクロマチンリモデリング因子DDM1によるエピジェネティックな制御がシロイヌナズナのヘテローシスに及ぼす影響Oral presentation
- 日本育種学会第130回講演会, Sep. 2016, Japanese, 鳥取大学, Domestic conferenceアブラナ科植物白さび病菌Albugo candidaに対する抵抗性遺伝子同定に向けたBrassica rapa抵抗性品種の選抜Oral presentation
- 園芸学会平成28年度秋季大会, Sep. 2016, Japanese, 名城大学, Domestic conferenceWhole genome bisulfite sequencingによるハクサイのDNAメチル化領域の探索Oral presentation
- 日本育種学会第130回講演会, Sep. 2016, Japanese, Domestic conferenceBrassica rapa L.の開花期の決定におけるFRIGIDAとFLOWERING LOCUS Cの役割Oral presentation
- 園芸学会平成28年度秋季大会, Sep. 2016, Japanese, 名城大学, Domestic conferenceBrassica rapa L.における春化によるFLOWERING LOCUS C のエピジェネティックな転写制御についてPoster presentation
- 園芸学会平成28年度秋季大会, Sep. 2016, Japanese, 名城大学, Domestic conferenceBrassica rapa L.における開花関連遺伝子FRIGIDA の発現解析および全長配列の決定Oral presentation
- 平成28 年度園芸学会近畿支部兵庫大会, Aug. 2016, Japanese, Domestic conferenceニホンナシS3-RNase 周辺領域451kb のゲノム構造解析Oral presentation
- 平成28 年度園芸学会近畿支部兵庫大会, Aug. 2016, Japanese, 神戸大学 瀧川記念学術交流会館, Domestic conferenceニホンナシS2-RNase 周辺領域663kb のゲノム構造解析Oral presentation
- 平成28 年度園芸学会近畿支部兵庫大会, Aug. 2016, Japanese, 神戸大学 瀧川記念学術交流会館, Domestic conferenceBrassica rapa における白さび病抵抗性遺伝子同定に向けた病害反応調査Oral presentation
- 平成28 年度園芸学会近畿支部兵庫大会, Aug. 2016, Japanese, Domestic conferenceBrassica rapa L.における春化経路関連遺伝子FLOWERING LOCUS C の発現解析および エピジェネティックな転写制御についてOral presentation
- 日本育種学会第129回講演会, Mar. 2016, Japanese, 横浜市立大学, Domestic conferenceシロイヌナズナの雑種強勢の分子機構の解明に向けたRILの整備Oral presentation
- 平成27年度園芸学会秋季大会, Sep. 2015, Japanese, 園芸学会, 徳島大学, 概要, Domestic conferenceハクサイにおけるヒストンメチル化抗体を用いたクロマチン免疫沈降法のポジティブ ネガティブコントロールプライマーの作製Oral presentation
- 園芸学会平成27年度秋季大会, Sep. 2015, Japanese, Domestic conferenceハクサイにおけるヒストンメチル化抗体を用いたクロマチン免疫沈降法のポジティブ/ネガティブコントロールプライマーの作製Oral presentation
- 日本育種学会第128回講演会, Sep. 2015, Japanese, Domestic conferenceハクサイにおいて、両親系統間の遺伝距離から雑種強勢を予測できるか?Oral presentation
- 育種学会第128回講演会, Sep. 2015, Japanese, 日本育種学会, 新潟大学, 概要, Domestic conferenceハクサイにおいて、両親系統間の遺伝距離から雑種強勢を予測できるか?Oral presentation
- 平成27年度秋季大会, Sep. 2015, Japanese, 園芸学会, 徳島大学, 概要, Domestic conferenceニホンナシSホモ系統の花粉cDNAからのPpSFBB2ホモログのクローニングIIOral presentation
- 日本遺伝学会第87大会, Sep. 2015, Japanese, 東北大学, Domestic conferenceアブラナ科の雑種強勢の分子機構の解明を目指した後成遺伝学的なアプローチ[Invited]Invited oral presentation
- 日本育種学会第128回講演会, Sep. 2015, Japanese, Domestic conferenceBrassica oleraceaの倍加半数体系統群を用いた連鎖地図の作成と根こぶ病レース特異的CR-QTLの同定Poster presentation
- 日本育種学会第128回講演会, Sep. 2015, Japanese, Domestic conferenceBrassica oleracea における根こぶ病抵抗性遺伝子PbBo(Anju)1 のファインマッピングOral presentation
- A consortium of plant epigenetisists in Japan – First Meeting, Jul. 2015, English, National Institute of Genetics, Domestic conferenceGenetic and epigenetic approaches for identification of molecular mechanism of heterosis in BrassicaPublic discourse
- Symposium; Epigenetics and Gene expression for Crop improvement, Apr. 2015, English, ANU university House, International conferenceDNA marker-assisted breeding in F1 hybrid cultivar of Chinese cabbageOral presentation
- Institute of Plant Science and Recourses Workshop; Plant Epigenetics: From Emerging Phenomena to Novel Molecular Events, Jan. 2015, English, Kurashiki Geibun kan, Domestic conferenceGenetic and epigenetic approaches for identification of molecular mechanism of heterosis in BrassicaOral presentation
- 日本育種学会第126回講演会, Sep. 2014, Japanese, 南九州大学, Domestic conferenceハクサイ市販一代雑種品種‘W77’における雑種強勢に関わるQTLの探索Oral presentation
- 平成26年度秋季大会, Sep. 2014, Japanese, 園芸学会, 佐賀大学, Domestic conferenceニホンナシ和合・不和合花粉管へのS-RNaseの取り込みOral presentation
- 平成26年度秋季大会, Sep. 2014, Japanese, 園芸学会, 佐賀大学, Domestic conferenceニホンナシSホモ系統の花粉cDNAからのPpSFBB2ホモログのクローニングOral presentation
- 平成26年度秋季大会, Sep. 2014, Japanese, 園芸学会, 佐賀大学, Domestic conferenceニホンナシS4-RNase周辺BACコンティグの拡張によるPpSFBB遺伝子群の探索Oral presentation
- 平成26年度秋季大会, Sep. 2014, Japanese, 園芸学会, 佐賀大学, Domestic conferenceニホンナシnon-S-RNaseの花柱組織における蓄積と花粉管への取り込みOral presentation
- 日本育種学会第126回講演会, Sep. 2014, Japanese, 南九州大学, Domestic conferenceシロイヌナズナの雑種強勢に概日リズムは関わるか?Poster presentation
- 日本育種学会第126回講演会, Sep. 2014, Japanese, 南九州大学, Domestic conferenceアブラナ科萎黄病菌(Fusarium oxysporum)のde novo全ゲノムシークエンス情報を用いた分化型特異的遺伝子領域の探索Poster presentation
- 日本育種学会第126回講演会, Sep. 2014, Japanese, 南九州大学, Domestic conferenceDNAマーカーを用いたBrassica rapa近交系の基盤整備Poster presentation
- 園芸学会平成26年度秋期大会, Sep. 2014, Japanese, Domestic conferenceBrassica oleracea近交系の遺伝的均一性評価およびF1種子純度検定に適したDNAマーカーの探索Poster presentation
- 園芸学会平成26年度春季大会, Mar. 2014, Japanese, 筑波大学, Domestic conferenceハクサイ市販一代雑種品種‘W39’における雑種強勢関連遺伝子の発現解析Oral presentation
- 日本育種学会第125回講演会, Mar. 2014, Japanese, 東北大学, Domestic conferenceハクサイ市販一代雑種品種W77及びそのF2後代の形質調査Oral presentation
- 日本育種学会第125回講演会, Mar. 2014, Japanese, 日本育種学会, Domestic conferenceシロイヌナズナの初期生育時に見られる雑種強勢はPol4の機能とは独立であるPoster presentation
- 日本育種学会第125回講演会, Mar. 2014, Japanese, 東北大学, Domestic conferenceRNA-sequencing法によるハクサイ市販F1品種の両親系統間のSNP同定Oral presentation
- 園芸学会平成26年度春季大会, Mar. 2014, Japanese, 筑波大学, Domestic conferenceDifferential expression解析を用いたハクサイ萎黄病抵抗性候補遺伝子の同定Poster presentation
- 日本育種学会第125回講演会, Mar. 2014, Japanese, 東北大学, Domestic conferenceBrassica oleraceaにおける近交系の純度検定に最適なDNAマーカーのスクリーニングと汎用性Poster presentation
- 日本育種学会第124回講演会, Oct. 2013, Japanese, 鹿児島大学, Domestic conferenceハクサイ市販F1品種‘W39’の生育初期に見られる雑種強勢についてOral presentation
- 日本育種学会第124回講演会, Oct. 2013, Japanese, 鹿児島大学, Domestic conferenceシロイヌナズナの子葉における雑種強勢を示すF1とその両親系統間での転写比較解析Oral presentation
- 日本育種学会第124回講演会, Oct. 2013, Japanese, 鹿児島大学, Domestic conferenceシロイヌナズナのF1雑種にみられる雑種強勢の程度についてOral presentation
- 日本育種学会第124回講演会, Oct. 2013, Japanese, 鹿児島大学, Domestic conferenceアブラナ科植物一代雑種品種における両親系統の対立遺伝子間相互作用Public symposium
- 日本育種学会第124回講演会, Oct. 2013, Japanese, 鹿児島大学, Domestic conferenceBrassica oleracea品種における萎黄病抵抗性遺伝子FocBo1連鎖DNA マーカーと抵抗性との関連性Poster presentation
- 日本育種学会第124回講演会, Oct. 2013, Japanese, 鹿児島大学, Domestic conferenceBrassica oleracea染色体添加型 B. rapa を用いた緑植物体春化の遺伝解析Oral presentation
- 園芸学会平成25年度秋期大会, Sep. 2013, Japanese, 岩手大学, Domestic conference初期生育時に見られるハクサイとシロイヌナズナの雑種強勢の相違点Poster presentation
- 園芸学会平成25年度秋期大会, Sep. 2013, Japanese, 岩手大学, Domestic conferenceハクサイF1品種W77における初期生育と収穫期の雑種強勢の評価Oral presentation
- 園芸学会平成25年度秋期大会, Sep. 2013, Japanese, 岩手大学, Domestic conferenceハクサイF1品種W39における生育初期及び収穫期の雑種強勢の評価Oral presentation
- 24th International Conference on Arabidopsis Research, Jun. 2013, English, Sydney, Australia, International conferenceVariation of the level of the heterosis among F1 hybrid individuals in Arabidopsis thalianaPoster presentation
- 24th International Conference on Arabidopsis Research, Jun. 2013, English, Sydney, Australia, International conferenceA large cotyledon area after germination is a common phenomenon in heterotic F1 hybrids of Arabidopsis thaliana and Chinese cabbagePoster presentation
- 日本育種学会第123回講演会, Mar. 2013, Japanese, 東京農業大学, Domestic conferenceアブラナ科植物の種内、種間雑種に見られる非相加的遺伝子発現[Invited]Invited oral presentation
- 第54回日本植物生理学会年会, Mar. 2013, English, 岡山大学, Domestic conferenceEstablishment of heterosis at early developmental stage in Arabidopsis thaliana is independent from Pol4 activityOral presentation
- 5th International symposium for the development of integrated pest management for sustainable agriculture in Asia and Africa, EnglishRecent progress of clubroot resistance breeding through marker-assisted selection in Brassica oleracea and B. rapaOral presentation
- 5th International symposium for the development of integrated pest management for sustainable agriculture in Asia and Africa, Dec. 2012, EnglishAnalysis of QTLs controlling black rot (Xanthomonas campestris pv. campestris) resistance in Brassica oleraceaOral presentation
- 5th International symposium for the development of integrated pest management for sustainable agriculture in Asia and Africa, Dec. 2012, EnglishIdentification of Brassica rapa fusarium wilt resistance gene, FocBr1, by differential expression analysis of NBS-LRR motif genesPoster presentation
- 6th International symposium on Brassica and 18th Crucifer Genetics Workshop, Nov. 2012, EnglishStrong Heterosis in Yield is Seen in Chinese Cabbage F1 Hybrid CultivarsPoster presentation
- 6th International symposium on Brassica and 18th Crucifer Genetics Workshop, Nov. 2012, EnglishIdentification of Brassica rapa homologue of fusarium wilt resistance gene, Foc-Bo1 by genome synteny analysis between B. oleracea and B. rapaPoster presentation
- International symposium on Comparative Genomics and Breeding of Brassicaceae crops, Oct. 2012, EnglishTowards cloning a clubroot resistance minor gene PbBo(Anju)4 in Brassica oleraceaPoster presentation
- International symposium on Comparative Genomics and Breeding of Brassicaceae crops, Oct. 2012, EnglishCollinearity among a short segment having the major recognition gene complex of Arabidopsis thaliana chromosome 4 and triplicated regions within the Brassica oleracea and B. rapaPoster presentation
- International symposium on Comparative Genomics and Breeding of Brassicaceae crops, Oct. 2012, EnglishNon-additive gene regulation in heterotic F1 hybrids of A. thalianaPoster presentation
- International symposium on Comparative Genomics and Breeding of Brassicaceae crops, Oct. 2012, EnglishCharacterization of the early developmental heterosis phenotype of the commercial F1 hybrid cultivars in Chinese cabbagePoster presentation
- 10th International Congress on Plant Molecular Biology, Oct. 2012, EnglishCharacterization of NBS-LRR motif genes between S11 and R09 by RNA sequencing in Brassica rapaPoster presentation
- 10th International Congress on Plant Molecular Biology, Oct. 2012, EnglishTowards identifying a fusarium wilt resistance gene in Chinese cabbage (Brassica rapa ssp. pekinensis)Poster presentation
- 日本育種学会, Sep. 2012on-additive gene regulation in heterotic F1 hybrids of Arabidopsis thaliana and Chinese cabbagePoster presentation
- 日本育種学会, Sep. 2012RNA-sequence法を用いたハクサイ品種間（S11×R09）特異的なNBS-LRRモチーフ遺伝子の探索Oral presentation
- The 4th International Symposium on ‘‘Modern Agriculture and Rural Economic Development in Asia’’, Jul. 2012, EnglishThe genetic association of clubroot and fusarium wild disease resistance gene between Brassica oleracea and Brassica rapa based on expression sequence tagged comparative genomics.Poster presentation
- The 4th International Symposium on ‘‘Modern Agriculture and Rural Economic Development in Asia’’, Jul. 2012, EnglishDevelopment of recombinant inbred line to clone three minor clubroot resistance genes in Brassica oleraceaPoster presentation
- The 4th International Symposium on ‘‘Modern Agriculture and Rural Economic Development in Asia’’, Jul. 2012, EnglishEarly developmental heterosis are observed in F1 hybrid cultivar ‘W39’ of Chinese cabbagePoster presentation
- The 4th International Symposium on ‘‘Modern Agriculture and Rural Economic Development in Asia’’, Jul. 2012, EnglishCharacterization of the heterosis phenotype of the commercial F1 hybrid cultivar ‘W77’ in Chinese cabbagePoster presentation
- 植物生理学会, Mar. 2012Stress response genes are differentially expressed both between related Arabidopsis species and in their amphidiploidPoster presentation
- 日本育種学会, Mar. 2012強いヘテローシスは、ハクサイの一代雑種品種の収量に見られるOral presentation
- 日本育種学会, Mar. 2012Brassica oleracea根こぶ病抵抗性遺伝子の集積による抵抗性育種Oral presentation
- 日本育種学会, Mar. 2012キャベツ萎黄病抵抗性遺伝子Foc-Bo1の同定とファインマッピングOral presentation
- XXII International Congress on Sexual Plant Reproduction. Melbourne, Feb. 2012, EnglishEpigenetics and heterosisOral presentation
- XXII International Congress on Sexual Plant Reproduction, Feb. 2012, EnglishEarly developmental heterosis in both Arabidopsis thaliana and Chinese cabbageOral presentation
- PLANT & ANIMAL GENOME XX, Jan. 2012, EnglishThe cumulative effect of five Clubroot resistant genes in Brassica oleraceaPoster presentation
- 日本育種学会, Sep. 2011シロイヌナズナの初期生育に見られるヘテローシスは開花時期とは独立であるPoster presentation
- 日本育種学会, Sep. 2011シロイヌナズナとハクサイにおいて、生育初期のシュートでヘテローシスが見られるOral presentation
- XVIII International botanical congress, Jul. 2011, EnglishEpigenetic and genetic variation in imprinted genes between Arabidopsis thaliana and Arabidopsis lyrataOral presentation
- 植物生理学会, Mar. 2011Arabidopsis属の雑種強勢が見られる2つの人工複二倍体間でのゲノムワイドな転写の比較Poster presentation
- 日本植物生理学会, Mar. 2011Epigenetic variation in the FWA gene within the genus ArabidopsisPoster presentation
- Combio2011, 2011, EnglishThe whole genome transcriptome suggests early events are associated with heteosis in Arabidopsis thalianaOral presentation
- Epigenetics and heterosis meeting, 2011, EnglishEarly developmental heterosis in both Arabidopsis thaliana and Chinese cabbageOral presentation
- 日本育種学会, Sep. 2010Arabidopsis属の人工複二倍体に見られる非相加的遺伝子制御と雑種強勢様の表現型Poster presentation
- 21st International Conference on Arabidopsis Research, Jun. 2010, EnglishDiversity of epigenetic control in the FWA gene within the genus ArabidopsisPoster presentation
- Combio 2009, Dec. 2009, EnglishGenome wide gene expression in artificially synthesized amphidiploids in ArabidopsisPoster presentation
- Epigenetics 2009, Dec. 2009, EnglishControl of FWA gene silencing in the genus ArabidopsisPoster presentation
- Combio 2009, Dec. 2009, EnglishHeterosis at early developmental stages in Arabidopsis thaliana is independent of flowering timeOral presentation
- Epigenome Conference, Sep. 2008, EnglishEvolution and metastable epigenetic states of imprinted FWA genes in ArabidopsisOral presentation
- 19th International conference on Arabidopsis research, Jul. 2008, EnglishIntra-specific variation of vegetative expression of imprinted FWA genePoster presentation
- XX International Congress of Genetics, Jul. 2008, EnglishEvolution of imprinted FWA genes in ArabidopsisPoster presentation
- 第2回日本エピジェネティクス研究会年会, May 2008栄養組織におけるインプリント遺伝子FWAの発現の同一種内での多様性Poster presentation
- NIBB-EMBL Joint Meeting: Evolution of epigenetic regulation, Mar. 2008, EnglishEvolution and control of imprinted FWA genes in ArabidopsisPoster presentation
- 日本育種学会, Mar. 2008Brassica rapaにおけるBrDDM1によるDNAメチル化制御の解析Oral presentation
- 特定領域研究「植物の生殖過程におけるゲノム障壁」若手研究者ワークショップ, Nov. 2007インプリント遺伝子FWAの構造と発現制御の進化Public symposium
- 日本育種学会, Sep. 2007Brassica rapaにおける器官特異的DNAメチル化の解析Poster presentation
- 日本育種学会, Sep. 2007Brassica属自家不和合性複合遺伝子座における組換えの検出と塩基配列多型への影響Oral presentation
- 日本遺伝学会, Sep. 2007シロイヌナズナのインプリント遺伝子FWAの進化Oral presentation
- 第1回日本エピジェネティクス研究会年会, Jun. 2007インプリント遺伝子FWAの構造と発現制御の進化：反復配列の役割についてPoster presentation
- The Fifth Okazaki Biology Conference: Speciation and Adaptation, Mar. 2007, EnglishSuppression of gene expression of a recessive SP11/SCR allele by an untranscribed SP11/SCR allele on same and different chromosomes in Brassica self-incompatibilityPoster presentation
- 日本植物生理学会, Mar. 2007Brassica rapaにおけるDDM1のDNAメチル化ターゲットの解析Poster presentation
- 2006年度遺伝研研究集会「高等植物の生殖形質におけるゲノム障壁制御遺伝子の分子遺伝学的解析」/特定領域研究「植物ゲノム障壁」ワークショップ, Nov. 2006Brassica rapaでの器官特異的な内生DNAメチル化制御と、BrDDM1によるDNAメチル化制御の解析Poster presentation
- 2006年度遺伝研研究集会「高等植物の生殖形質におけるゲノム障壁制御遺伝子の分子遺伝学的解析」/特定領域研究「植物ゲノム障壁」ワークショップ, Nov. 2006アブラナ科植物の自家和合性変異体の和合性変異の解析Poster presentation
- Sep. 2006Brassica rapa の自家和合性品種Yellow sarsonの起源Sハプロタイプの同定とS遺伝子座のゲノム構造の変異Poster presentation
- 日本育種学会, Sep. 2006アブラナ科植物種におけるレトロトランスポゾンの系統解析Poster presentation
- 日本育種学会, Sep. 2006Brassica rapaのddm1-RNAi形質転換体における低メチル化の遺伝についてPoster presentation
- 日本育種学会, Sep. 2006Brassica rapaにおけるddm1 RNAi形質転換体の作出とDDM1のメチル化ターゲットの解析Oral presentation
- XIX th International Congress on Sexual Plant Reproduction, Jul. 2006, EnglishAn alloplasmic male-sterile line of Brassica oleracea harboring the mitochondria from Diplotaxis muralis expresses a novel chimeric open reading frame, orf72Poster presentation
- XIX th International Congress on Sexual Plant Reproduction, Jul. 2006, EnglishSuppression of gene expression of a recessive SP11/SCR allele by an untranscribed SP11/SCR allele in Brassica self-incompatibilityPoster presentation
- 日本植物生理学会, Mar. 2006Brassica rapaにおけるDNAメチルトランスフェラーゼ遺伝子の解析Poster presentation
- 日本分子生物学会, Dec. 2005Brassica rapaにおける低メチル化形質転換体の解析Poster presentation
- 岩手大学-東北大学ジョイント国際シンポジウム, Aug. 2005アブラナ科F1品種の自殖分離集団における自家不和性強度の評価Poster presentation
- 日本育種学会, Aug. 2005SLGからSRKへのgene conversionによって生じた自家和合性の解析Poster presentation
- Plant Biology, Jul. 2005, EnglishSelf-compatibility caused by a gene conversion between SRK and SLG of the same S haplotypePoster presentation
- 日本植物生理学会, Mar. 2005アブラナ科自家和合性系統の和合性突然変異の解明Poster presentation
- 2004年度遺伝研研究集会「高等植物の生殖システム統御機能の分子遺伝学的解析」, Nov. 2004自家和合性Brassica rapa L. var yellow sarsonのSRK、SP11の遺伝子構造Public symposium
- Joint Meeting of the 14th Crucifer Genetics Workshop and the 4th ISHS Symposium on Brassica., Oct. 2004, EnglishGene structure of SRK and SP11/SCR in self-compatible B. rapa L. var. yellow sarsonPoster presentation
- 日本遺伝学会, Sep. 2004自家不和合性の認識特異性とS遺伝子座の進化Nominated symposium
- 日本育種学会, Sep. 2004Brassica rapa種内においてSRK、SLGが類似するSハプロタイプのゲノム構造の比較Oral presentation
- XVIIIth International Congress on Sexual Plant Reproduction, Aug. 2004, EnglishComparison of the genome structure of the self-incompatibility (S) locus in interspecific pairs of S haplotypesPoster presentation
- XVIIIth International Congress on Sexual Plant Reproduction, Aug. 2004, EnglishDiversification and evolution of S haplotypes revealed by the study of Brassica and Rhaphanus self-incompatibility[Invited]Invited oral presentation
- 日本育種学会, Apr. 2004Brassica oleraceaとBrassica rapaの自家不和合性クラスIIS遺伝子座のゲノム構造の比較Oral presentation
- International symposium on plant self-incompatibility, Sep. 2003, EnglishCommonality of self-recognition specificity of S haplotypes between Brassica oleracea and Brassica rapaPoster presentation
- International symposium on plant self-incompatibility, Sep. 2003, EnglishGenomic organization of the S core region and the S flanking regions of a class-II S haplotype in Brassica rapaPoster presentation
- International symposium on plant self-incompatibility, Sep. 2003, EnglishComparison of self-incompatibility (S ) locus between Brassica oleracea and Brassica rapa, and diversity of the retrotransposons found in the S locus region of Brassica oleraceaPoster presentation
- Sep. 2003Brassica oleraceaとBrassica rapaの自家不和合性(S)遺伝子座の比較、及びBrassica oleraceaに存在するレトロトランスポゾンの多様性Oral presentation
- Plant Biology, Jul. 2003, EnglishGenomic organization of the S core region and the S flanking regions of a class-II S haplotype in Brassica rapaPoster presentation
- Plant Biology, Jul. 2003, EnglishComparison of genome structure of classⅠ self-incompatibility (S) locus between Brassica oleracea and Brassica rapaPoster presentation
- 日本育種学会, Apr. 2003Brassica oleraceaとB. rapaのSハプロタイプが有する自己認識特異性の共通性Oral presentation
- 日本育種学会, Sep. 2002Brassica rapa自家不和合性クラスIIS遺伝子座のゲノム構造解析Oral presentation
- 日本育種学会, Sep. 2002Brassica oleracea及びBrassica rapaの自家不和合性クラスIS遺伝子座のゲノム構造の比較Oral presentation
- 日本育種学会, Mar. 2002SP11を用いたドットブロット分析によるSハプロタイプ判定Oral presentation
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Yamaguchi University, 01 Apr. 2024 - 31 Mar. 2027Elucidation of molecular mechanism of chiasma localization in bunching onion and its application to bulb onion breeding
- 日本学術振興会, 科学研究費助成事業, 基盤研究(B), 神戸大学, 01 Apr. 2022 - 31 Mar. 2026アブラナ科野菜の雑種強勢における顕性遺伝子座の役割の解明
- 科学技術振興機構, 産学が連携した研究開発成果の展開 研究成果展開事業 研究成果最適展開支援プログラム(A-STEP) 産学共同(育成型), 神戸大学, 2023 - 2025アブラナ科葉・根菜類では、栽培中に低温にさらされると、花成誘導・とう立ち(抽だい)が起こり、食味等の品質が低下するため、栽培中に低温にさらされても花成誘導されにくい晩抽性に優れた品種が望まれている。しかし、晩抽性の能力を高めると花を咲かせるのに必要な低温処理期間が長くなることから、品種育成の効率化を妨げるといったジレンマが存在する。そこで本研究では、遺伝子修飾を巧みに利用することで、晩抽性を自在に制御する技術の確立を目指す。これにより、従来法とは異なった育種システムの構築が期待でき、育種法の幅を広げることが期待できる。本技術シーズを元に、産学連携へと発展させ、品種育成への導入を目指す。
- 日本学術振興会, 科学研究費助成事業, 基盤研究(B), 新潟大学, 01 Apr. 2021 - 31 Mar. 2024ブラシカ属作物の春化機構における種内変異，種間変異の遺伝解析とエピゲノム解析
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2019 - 31 Mar. 2023Comprehensive analysis SFBB for S-RNase in self-incompatibility of Japanese pearナシ属の自家不和合性による花粉管の伸長抑制は雌ずい側因子S-RNaseが花粉管内のRNAを分解することで起こる。一方, 和合花粉管内では花粉側因子であるF-boxタンパク質群（SFBB）によりS-RNaseが無毒化されると考えられている。本研究では, SハプロタイプにコードされるSFBB群を掌握することで, S-RNaseに対する特異性を推定する。その推定をペチュニアの花粉で発現させたSFBB融合タンパク質のS-RNaseに対する結合特性を検証することを目的とした。 令和元年度は, ニホンナシのS2, S3, S4 BACコンティグ内外に存在する未解読BACクローンを次世代シークエンサーPacBioRSIIで解析し, 取得した配列を既知のコンティグに統合した。コンティグ配列はS2-RNase上流578kb~下流481kb, S3-RNase上流824kb~下流471kb, S4-RNase上流935kb~下流538kbまで拡張され, それぞれ18個, 19個, 20個のSFBBが同定された。各コンティグの両末端にはSFBB様配列は見出せなかったことから, これらSハプロタイプがコードするSFBB群を掌握できた。RNA-seqデータから構築したS1,S5-SFBB群配列の非翻訳領域から設計したプライマーセットを用いたRT-PCRにより未だクローニングされていないSFBB群配列をクローニングし, 配列を確認した。一方で, Skを有するニホンナシ品種 ‘巾着’の花粉をRNA-seq解析して, Sk-SFBB群配列を構築した。 S4-SFBB4d1およびその対立遺伝子のS1-SFBB4d1をそれぞれ導入したペチュニア形質転換を作出し, RT-PCRにより花粉での発現を確認した。 電子線トモグラフィー解析に向けた技術的な課題（試料作製法および連続傾斜撮影法など）を洗い出した。
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), Kobe University, 30 Jul. 2020 - 31 Mar. 2022A challenge to modify the vernalization requirement of root and leaf vegetables of BrassicaThis study aims to artificially control the low-temperature requirement of Brassica vegetables. In this study, we found four extremely late-bolting lines, one of which had an insertion in the first intron of the BrFLC2 or BrFLC3 gene. These inserted regions were highly DNA methylated. We suggest that DNA methylation in the inserted regions of these two genes are the possible cause of the decreased cold response. We also constructed a vector for epigenome editing using Arabidopsis thaliana to modify histone modifications at the FLC locus.
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2019 - 31 Mar. 2022Elucidation of the molecular mechanism of hybrid vigor in Brassica vegetablesTo elucidate the molecular mechanism of hybrid vigor in Chinese cabbage, we performed genetic, genome-wide association, and whole genome DNA methylation analyses. In the genetic analysis, we identified QTLs that are related leaf area at 14 days after sowing or yield. Whole genome DNA methylation analysis identified regions where DNA methylation levels differed between the parental lines and their F1 progenies. For genome-wide association analysis, RNA-seq was performed on 73 F1 hybrid cultivars to obtain polymorphic information. In addition, mutants of genes involved in DNA methylation were generated by genome editing.
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Niigata University, 01 Apr. 2018 - 31 Mar. 2021Study on vernalization mechanism of Brassica crops using newly developed Intromap, a bioinformatic tool to identify alien genomic introgression regionsIn order to elucidate the vernalization mechanism of the genus Brassica, we did the following studies. We produced C-genome chromosome addition lines using BoFLC2-introgressed B. rapa as a recurrent parent and a cabbage as a c-genome chromosome donor, and characterized those lines with respect to the parental cultivars. In Chinese cabbage, the epigenetic regulation of gene expression at the FLC locus was clarified by RNA-seq, lncRNA analysis, and chromatin immunoprecipitation analysis. In addition, transcriptome analysis was performed on the leaves of the cabbage DH strain with/without low temperature treatment. QTL analysis was performed to determine vernalization characteristics of rapeseed cultivars using F2 populations derived from the crossing between seed vernalization typed rapeseed x green plant vernalization typed rapeseed.
- 学術研究助成基金助成金／国際共同研究加速基金(国際共同研究強化), Apr. 2017 - Mar. 2020, Principal investigatorCompetitive research funding
- 科学研究費補助金／基盤研究(B), Apr. 2015 - Mar. 2019Competitive research funding
- 科学研究費補助金／若手研究(A), Apr. 2015 - Mar. 2019, Principal investigatorCompetitive research funding
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Niigata University, 01 Apr. 2015 - 31 Mar. 2018Study on the mechanism of seed- and green plant-vernalization and development of green plant-vernalization type Chinese cabbageWe characterized vernalization requirement of 49 strains of oilseed rape, and developed the F2 population of Isuzu-natane (low vernalization requirement) × synthetic B. napus (strong vernalization requirement ). In addition, we developed a line that was introduced the region containing BoFLC 2 region derived from cabbage into the top of chromosome 2 of Chinese cabbage. We showed that the differences of expression level of BrFLC 1/2/3 in cabbage and Chinese cabbage may be related to the diversity of vernalization requirement in cabbage and Chinese cabbage cultivars. The RNA-seq analysis revealed that long non coding RNA was expressed on BoFLC2 locus by low temperature treatment. Our developed Chinese cabbage line showed delay of flowering in the winter cropping pattern, compared with ordinary Chinese cabbage cultivars, indicating that it can be used as a breeding material.
- 独立行政法人日本学術振興会, 二国間国際交流（オーストラリア(OP)との共同研究）, 2017, Principal investigatorアブラナ科植物におけるDNAメチル化による雑種強勢発現機構の解明Competitive research funding
- 研究成果展開事業（マッチングプランナー プログラム）, 2016, Principal investigatorアブラナ科野菜の白さび病抵抗性遺伝子の同定Competitive research funding
- 独立行政法人日本学術振興会, 二国間交流事業（オープンパートナーシップ共同研究）, 2016, Principal investigatorアブラナ科植物のヘテローシスの分子機構の解析Competitive research funding
- 日本学術振興会, 二国間交流事業（オープンパートナーシップ共同研究）, 2015, Principal investigator二国間交流「アブラナ科植物のヘテローシスの分子機構の解析」Competitive research funding
- 科学技術振興機構, 戦略的創造研究推進事業 個人型研究さきがけ, 2015, Principal investigatorさきがけ「雑種強勢の分子機構の解明とその高バイオマス作物への活用」Competitive research funding
- 学術研究助成基金助成金／若手研究(B), Apr. 2012 - Mar. 2014, Principal investigatorCompetitive research funding
- 科学研究費補助金／新学術領域研究, Apr. 2012 - Mar. 2014, Principal investigatorCompetitive research funding
- 二国間交流事業（オープンパートナーシップ共同研究）, 2014, Principal investigator二国間交流「アブラナ科植物のヘテローシスの分子機構の解析」Competitive research funding
- 戦略的創造研究推進事業 個人型研究さきがけ, 2014, Principal investigatorさきがけ「雑種強勢の分子機構の解明とその高バイオマス作物への活用」Competitive research funding
- 戦略的創造研究推進事業 個人型研究さきがけ, 2013, Principal investigatorさきがけ「雑種強勢の分子機構の解明とその高バイオマス作物への活用」Competitive research funding
- 日本学術振興会, 科学研究費助成事業, 特別研究員奨励費, 東北大学, 2003 - 2005アブラナ科植物の自家不和合性遺伝子座に存在するレトロトランスポゾンの発現解析アブラナ科植物の自家不和合性は一つの遺伝子座(S)を想定することで説明されている。自家不和合性反応はS遺伝子座に遺伝子が座乗する柱頭側の認識分子SRKと花粉側の認識分子SP11の相互作用による。同じくS遺伝子座にあるSLGは、SRKの細胞外ドメインと相同性が見られる。F_1品種‘CR清雅65'(S-46/S-54)の自殖分離集団の中から、5つの自家和合性個体(S-54ホモ)が得られた。S-54の自家和合個体と自家不和合個体の相互交配の結果、和合性の原因が柱頭側にあることが明らかとなった。連鎖解析から和合性の要因がSと連鎖していることが明らかになった。柱頭側の認識因子であるSRKの発現量に差は見られなかったが、SLGからSRKへのgene conversionが生じていた。以上の結果から、自家和合性の原因は、gene conversionによって生じたSRKがSP11を認識できなくなったためであると考えた。 アブラナ科植物から、DNAのメチル化に関与すると考えられる遺伝子、BrMET1a、BrMET1b、BrCMT、BrDDM1を単離した。このうち、BrDDM1の配列を元にRNAiのコンストラクトを作成し、形質転換体の作成を行い、11個の独立した形質転換体を得た。形質転換体ではメチル化の程度の低下が確認され、その程度は個体間で異なった。メチル化の低下の程度が1番大きかった形質転換体の表現型はWTと変わらなかったが、WTで発現しないことを確認しているレトロトランスポゾンの発現が誘導されていた。このレトロトランスポゾンのメチル化程度を形質転換体とWTで比較したところ、形質転換体でDNAのメチル化の程度が低下していたことから、このレトロトランスポゾンの発現誘導は、メチル化レベルの低下により起こったと考えられた。