Research activity information

• Inactivation of SARS-CoV-2 and influenza A virus by dry fogging hypochlorous acid solution and hydrogen peroxide solution

  Masahiro Urushidani, Akira Kawayoshi, Tomohiro Kotaki, Keiichi Saeki, Yasuko Mori, Masanori Kameoka

  Public Library of Science (PLoS), Apr. 2022, PLOS ONE, 17(4) (4), e0261802 - e0261802

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Increased expression of prion protein gene is accompanied by demethylation of CpG sites in a mouse embryonal carcinoma cell line, P19C6

  Wuyun Dalai, Eiko Matsuo, Natsumi Takeyama, Junichi Kawano, Keiichi Saeki

  Mar. 2017, JOURNAL OF VETERINARY MEDICAL SCIENCE, 79(3) (3), 644 - 648, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• CpG site DNA methylation patterns reveal a novel regulatory element in the mouse prion protein gene

  Wuyun Dalai, Eiko Matsuo, Natsumi Takeyama, Junichi Kawano, Keiichi Saeki

  Jan. 2017, JOURNAL OF VETERINARY MEDICAL SCIENCE, 79(1) (1), 100 - 107, English

  [Refereed]

  Scientific journal


• Development of reverse genetics for Ibaraki virus to produce viable VP6-tagged IBAV

  Eiko Matsuo, Keiichi Saeki, Polly Roy, Junichi Kawano

  2015, FEBS OPEN BIO, 5, 445 - 453, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Development of a New Selective Medium for Isolation of Methicillin-Resistant Staphylococcus aureus

  Etsuko Saito, Yoko Kakita, Hiroyuki Shimoda, Shizuka Shimabukuro, Keiichi Saeki, Akira Shimizu, Junichi Kawano

  Sep. 2011, JOURNAL OF VETERINARY MEDICAL SCIENCE, 73(9) (9), 1195 - 1197, English

  [Refereed]

  Scientific journal


• Localization of insulinoma associated protein 2, IA-2 in mouse neuroendocrine tissues using two novel monoclonal antibodies

  Natsumi Takeyama, Yasuhisa Ano, Guoying Wu, Nobuyoshi Kubota, Keiichi Saeki, Akikazu Sakudo, Eiichi Momotani, Katsuaki Sugiura, Masayoshi Yukawa, Takashi Onodera

  May 2009, LIFE SCIENCES, 84(19-20) (19-20), 678 - 687, English

  [Refereed]

  Scientific journal


• 正常プリオン蛋白質におけるアポトーシス抑制機能部位に関する研究

  佐伯圭一, 作道章一, 松本芳嗣, 小野寺節

  Aug. 2008, Bull Soc Fr-Jpn Biol, 45/46, 37, Japanese


• Adhesion of MRP8/14 to amtastigotes in skin lesions of Leishmania major-infected mice

  Yasuyuki Goto, Chizu Sanjoba, Masahito Asada, Keiichi Saeki, Takashi Onodera, Yoshitsugu Matsumoto

  May 2008, EXPERIMENTAL PARASITOLOGY, 119(1) (1), 80 - 86, English

  [Refereed]

  Scientific journal


• Accumulation of macrophages expressing MRP8 and MRP14 in skin lesions during Leishmania major infection in BALB/c and RAG-2 knockout mice.

  Goto Y, Sanjoba C, Arakaki N, Okamoto M, SAEKI KEIICHI, Onodera T, Ito M, Matsumoto Y

  Mar. 2007, Parasitol Int., 56(3) (3), 231 - 234, English

  [Refereed]

  Scientific journal


• Late-onset olfactory deficits and mitral cell loss in mice lacking prion protein with ectopic expression of Doppel.

  Kim CK, Sakudo A, Taniuchi Y, Shigematsu K, Kang CB, SAEKI KEIICHI, Matsumoto Y, Sakaguchi S, Itohara S, Onodera T

  Feb. 2007, Int. J. Mol. Med., 20(2) (2), 169 - 176, English

  [Refereed]

  Scientific journal


• Detection of proteolytic cleavages of diabetes-associated protein IA-2beta in the pancreas and the brain using novel anti-IA-2beta monoclonal antibodies.

  Kawakami T, SAEKI KEIICHI, Takeyama N, Wu G, Sakudo A, Matsumoto Y, Hayashi T, Onodera T

  Feb. 2007, Int. J. Mol. Med., 20(2) (2), 177 - 185, English

  [Refereed]

  Scientific journal


• Abnormal olfactory function caused by ectopic expression of Doppel in the olfactory bulb of prion protein-deficient mice.

  Kim, C.K, Sakudo, A, Taniuchi, Y, Kang, C.B, Lee, D.C, Saeki, K, Matsumoto, Y, Sakaguchi, S, Itohara, S, Onodera, T

  2007.

  2007, Int. J. Mol. Med., 20, 169-176.


• Lack of alternations of IA-2β protevtive cleavages in non-obese diabetic (NOD) mice.

  Kawakami, T, Saeki, K, Takeyama, N, Wu, G.Y, Sakudo, A, Matsumoto, Y, Hayashi, T, Onodera, T

  2007.

  2007, Int. J. Mol. Med., 20, 177-185.


• Neurotoxic prion protein (PrP) fragment 106-126 requires the N-terminal half of the hydrophobic region of PrP in the PrP-deficient neuronal cell line.

  Akikazu Sakudo, Izuru Nakamura, Deug-Chan Lee, Keiichi Saeki, Kazuyoshi Ikuta, Takashi Onodera

  The cytotoxicity of aged PrP(106-126) was examined using an immortalized prion protein (PrP) gene-deficient neuronal cell line. The N-terminal half of the hydrophobic region (HR) but not the octapeptide repeat (OR) of PrP was required for aged PrP(106-126) neurotoxicity, suggesting that neurotoxic signals of aged PrP(106-126) are mediated by this region.

  2007, Protein and peptide letters, 14(1) (1), 1 - 6, English, International magazine

  [Refereed]

  Scientific journal


• Novel single nucleotide polymorphisms in the specific protein 1 binding site of the bovine PRNP promoter in Japanese Black cattle: Impairment of its promoter activity

  Izuru Nakamura, Guangai Xue, Akikazu Sakudo, Keiichi Saeki, Yoshitsugu Matsumoto, Kazuyoshi Ikuta, Takashi Onodera

  2007, INTERVIROLOGY, 50(3) (3), 190 - 196, English

  [Refereed]

  Scientific journal


• Serum withdrawal-induced apoptosis in ZrchI prion protein (PrP) gene-deficient neuronal cell line is suppressed by PrP, independent of Doppel.

  Takuya Nishimura, Akikazu Sakudo, Yoriko Hashiyama, Akiko Yachi, Keiichi Saeki, Yoshitsugu Matsumoto, Masaharu Ogawa, Suehiro Sakaguchi, Shigeyoshi Itohara, Takashi Onodera

  Previous studies have shown that cellular prion protein (PrP(C)) plays anti-apoptotic and antioxidative role against cell death induced by serum-deprivation (SDP) in an immortalized prion protein gene-deficient neuronal cell line derived from Rikn prion protein (PrP) gene-deficient (Prnp(-/-)) mice, which ectopically produce excess Doppel (Dpl) (PrP-like glycoprotein). To investigate whether PrP(C) inhibits apoptotic neuronal cell death without Dpl, an immortalized cell line was established from the brain of ZrchI Prnp(-/-) mice, which do not show ectopic expression of Dpl. The results using a ZrchI neuronal Prnp(-/-) cell line (NpL2) showed that PrP(C) potently inhibited SDP-induced apoptotic cell death. Furthermore, PrP(C) expression enhanced the superoxide dismutase (SOD) activity in NpL2 cells. These results indicate that Dpl production did not affect anti-apoptotic and anti-oxidative functions of PrP, suggesting that PrP(C) may be directly correlated with protection against oxidative stress.

  2007, Microbiology and immunology, 51(4) (4), 457 - 66, English, International magazine

  [Refereed]

  Scientific journal


• Recent advances in clarifying prion protein functions using knockout mice and derived cell lines

  A Sakudo, T Onodera, Y Suganuma, T Kobayashi, K Saeki, K Ikuta

  May 2006, MINI-REVIEWS IN MEDICINAL CHEMISTRY, 6(5) (5), 589 - 601, English

  [Refereed]

  Scientific journal


• Fusion of Doppel to octapeptide repeat and N-terminal half of hydrophobic region of prion protein confers resistance to serum deprivation

  DC Lee, A Sakudo, CK Kim, T Nishimura, K Saeki, Y Matsumoto, T Yokoyama, SG Chen, S Itohara, T Onodera

  2006, MICROBIOLOGY AND IMMUNOLOGY, 50(3) (3), 203 - 209, English

  [Refereed]

  Scientific journal


• BSEの分子生物学

  小野寺節、作道章一、佐伯圭一、谷内洋次郎、中村出

  2006, 医薬ジャーナル, 22, 49 - 54, Japanese

  Scientific journal


• Bovine spongiform encephalopathy in Japan: history and recent studies on oxidative stress in prion diseases.

  Takashi Onodera, Akikazu Sakudo, Guoying Wu, Keiichi Saeki

  With the respect to BSE and vCJD, compliance with the following three rules should strictly be observed: (i) Identification and destruction of all clinically affected cattle; (ii) destruction of all mammalian proteins used in feeding ruminant livestock; and (iii) destruction of all high-risk tissues for use in human consumption. Scrapie in sheep has been documented in the 18th century in the United Kingdom. Through studies of brain-to-brain transmission in the same species in 1935, Cuille et al. successfully isolated the culprit protein from the sheep brain. To transmit said protein from an animal to another, intracerebral inoculation was much more efficient than intraperitoneal or oral route in certain species; i.e. the hamster and mouse. Since discovery of the more efficacious infection route, studies and development of prion research have undergone 4 developmental phases. Phase I depicted discoveries of the pathological features of Creutzfeldt-Jakob disease (CJD) and scrapie with typical lesions of spongiform encephalopathy, while Phase II revealed individual-to-individual (or cross-species) transmissions of CJD, kuru and scrapie in animals. Phases I and II suggested the possible participation of a slow virus in the infection process. In Phase III, Prusiner et al. proposed the 'prion' theory in 1982, followed by the milestone development of the transgenic or gene-targeted mouse in prion research in Phase IV. By strain-typing of prions, CJD has been classified as type 2 or 4 by Parchi et al. and Wadsworth as type-2 or -4 and type-1 or -2, respectively. Wadsworth type 1 is detected in the cerebellum, while Wadsworth type 2 was detected in the prefrontal cortex of 10% of sporadic CJD patients. In 1999, Puoti et al. have reported the co-existence of two types of PrP(res) in a same patient. These reports indicated that PrP(res)-typing is a quantitative rather than a qualitative process, and the relationship between the molecular type and the prion strain is rather complex. In fact, previous findings of Truchot have correlated type-1 distribution with synaptic deposits, and type-2 with arrangement of diffuse deposits in neurons. Although the normal function of PrP(C) has not been fully understood, recent studies have shown that PrP(C) plays a role in copper metabolism, signal transduction, neuroprotection and cell maturation. Further search of PrP(C)-interacting molecules and detailed studies using Prnp(-/-) mice and various type of Prnp(-/-) cell lines under various conditions are the prerequisites in elucidating PrP functions. In the pathogenesis of prion diseases, present results support the hypothesis that 'loss-of-function' of PrP(C) decreases resistance to oxidative stress, and 'gain-of-function' of PrP(Sc) increases oxidative stress. The mechanisms of (i) the 'loss-of-function' of PrP(C) in enhanced susceptibility to oxidative stress and (ii) the 'gain-of-function' of PrP(Sc) in generation of oxidative stress remain to be elucidated, although their mechanisms of action, at least in part, involve the decrease and increase in SOD activity, respectively.

  Last, 2006, Microbiology and immunology, 50(8) (8), 565 - 78, English, International magazine

  [Refereed]

  Scientific journal


• Serological and immunohistochemical studies on porcine-serum-induced hepatic fibrosis in rats

  Y Baba, K Saeki, T Onodera, K Doi

  Dec. 2005, EXPERIMENTAL AND MOLECULAR PATHOLOGY, 79(3) (3), 229 - 235, English

  [Refereed]

  Scientific journal


• 正常型プリオンタンパクの抗アポト—シス機能における構造的な機能中心の解析

  李 得燦, 作道 章一, 佐伯 圭一, 小野寺 節

  獣医生化学会, Dec. 2005, Veterinary biochemistry, 42, 19 - 23, Japanese

  Scientific journal


• Cell-autonomous PrP-Doppel interaction regulates apoptosis in PrP gene-deficient neuronal cells

  A Sakudo, DC Lee, Nakamura, I, Y Taniuchi, K Saeki, Y Matsumoto, S Itohara, K Ikuta, T Onodera

  Jul. 2005, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 333(2) (2), 448 - 454, English

  [Refereed]

  Scientific journal


• Hypoglycemia enhances the expression of prion protein and heat-shock protein 70 in a mouse neuroblastoma cell line

  WC Shyu, CP Chen, K Saeki, A Kubosaki, Y Matusmoto, T Onodera, DC Ding, MF Chiang, YJ Lee, SZ Lin, H Li

  Jun. 2005, JOURNAL OF NEUROSCIENCE RESEARCH, 80(6) (6), 887 - 894, English

  [Refereed]

  Scientific journal


• Activation of phosphatidylinositol in 3-kinase by cellular prion protein and its role cell survival

  N Vassallo, J Herms, C Behrens, B Krebs, K Saeki, T Onodera, O Windl, HA Kretzschmar

  Jun. 2005, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 332(1) (1), 75 - 82, English

  [Refereed]

  Scientific journal


• PrP cooperates with STI1 to regulate SOD activity in PrP-deficient neuronal cell line.

  Sakudo A, Lee DC, Li S, Nakamura T, Matsumoto Y, Saeki K, Itohara S, Ikuta K, Onodera T.

  May 2005, Biochem Biophys Res Commun., 328(1) (1), 14 - 19, English

  [Refereed]

  Scientific journal


• Infection route-independent accumulation of splenic abnormal prion protein.

  Inoue Y, Yamakawa Y, Sakudo A, Kinumi T, Nakamura Y, Matsumoto Y, Saeki K, Kamiyama T, Onodera T, Nishijima M.

  Mar. 2005, Jpn J Infect Dis, 58(2) (2), 78 - 82, English

  [Refereed]

  Scientific journal


• Cellular prion protein suppressess the apoptotic cell death by mediating the intracellular H2O2 in primary culture and immortalized neuronal cells

  Nakamura, I, T Nishimura, K Saeki, Y Matsumoto, T Onodera

  2005, Prions: Food and Drug Safety, 205 - 205, English

  International conference proceedings


• Cellular prion protein suppresses the apoptosis in a neuronal cell line established from type-1 prion protein gene-deficient mice

  K Saeki, T Nishimura, A Sakudo, Y Matsumoto, T Onodera

  Lead, 2005, Prions: Food and Drug Safety, 203 - 203, English

  International conference proceedings


• 動物の検疫および水際対策 : 牛海綿状脳症(BSE)に関する防疫措置

  佐伯 圭一, 小野寺 節

  2005, 日本水産學會誌, 69(2) (2), 257 - 258, Japanese

  Symposium


• Octapeptide repeat region and N-terminal half of hydrophobic region of prion protein (PrP) mediate PrP-dependent activation of superoxide dismutase

  A Sakudo, DC Lee, T Nishimura, SM Li, S Tsuji, T Nakamura, Y Matsumoto, K Saeki, S Itohara, K Ikuta, T Onodera

  Jan. 2005, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 326(3) (3), 600 - 606, English

  [Refereed]

  Scientific journal


• Cellular prion protein regulates intracellular hydrogen peroxide level and prevents copper-induced apoptosis

  T Nishimura, A Sakudo, Nakamura, I, DC Lee, Y Taniuchi, K Saeki, Y Matsumoto, M Ogawa, S Sakaguchi, S Itohara, T Onodera

  Oct. 2004, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 323(1) (1), 218 - 222, English

  [Refereed]

  Scientific journal


• Targeted disruption of the IA-2 beta gene causes glucose intolerance and impairs insulin secretion but does not prevent the development of diabetes in NOD mice

  A Kubosaki, S Gross, J Miura, K Saeki, M Zhu, S Nakamura, W Hendriks, AL Notkins

  Jul. 2004, DIABETES, 53(7) (7), 1684 - 1691, English

  [Refereed]

  Scientific journal


• Hyperbaric oxygen enhances the expression of prion protein and heat shock protein 70 in a mouse neuroblastoma cell line

  WC Shyu, SZ Lin, K Saeki, A Kubosaki, Y Matsumoto, T Onodera, MF Chiang, P Thajeb, H Li

  Apr. 2004, CELLULAR AND MOLECULAR NEUROBIOLOGY, 24(2) (2), 257 - 268, English

  [Refereed]

  Scientific journal


• Effects of tacrolimus (FK506) on encephalomyocarditic virus-induced diabetes in mice

  S Yama, W Nishioka, Y Hirokami, R Setoguchi, N Takeyama, K Saeki, Y Matsumoto, T Hayashi, K Doi, T Onodera

  2004, MICROBIOLOGY AND IMMUNOLOGY, 48(1) (1), 7 - 13, English

  [Refereed]

  Scientific journal


• Prion protein suppresses perturbation of cellular copper homeostasis under oxidative conditions

  A Sakudo, DC Lee, E Yoshimura, S Nagasaka, K Nitta, K Saeki, Y Matsumoto, S Lehmann, S Itohara, S Sakaguchi, T Onodera

  Jan. 2004, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 313(4) (4), 850 - 855, English

  [Refereed]

  Scientific journal


• Serum thymic factor prevents LPS-induced pancreatic cell damage in mice via up-regulation of Bcl-2 expression in pancreas

  N Saitoh, A Awaya, A Sakudo, SS Wook, K Saeki, Y Matsumoto, T Onodera

  2004, MICROBIOLOGY AND IMMUNOLOGY, 48(9) (9), 629 - 638, English

  [Refereed]

  Scientific journal


• Transfection of prion protein gene suppresses coxsackievirus B3 replication in prion protein gene-deficient cells

  Y Nakamura, A Sakudo, K Saeki, T Kaneko, Y Matsumoto, A Toniolo, S Itohara, T Onodera

  Dec. 2003, JOURNAL OF GENERAL VIROLOGY, 84, 3495 - 3502, English

  [Refereed]

  Scientific journal


• Suppressor mechanism of serum thymic factor on tumor necrosis factor-alpha-induced apoptosis in the mouse pancreatic beta-cell line

  J Yasuda, W Nishioka, A Sakudo, S Yama, R Setoguchi, K Saeki, Y Matsumoto, A Awaya, T Onodera

  Nov. 2003, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 311(2) (2), 501 - 505, English

  [Refereed]

  Scientific journal


• Tumor necrosis factor attenuates prion protein-deficient neuronal cell death by increases in anti-apoptotic Bcl-2 family proteins

  A Sakudo, DC Lee, K Saeki, Y Matsumoto, S Itohara, T Onodera

  Oct. 2003, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 310(3) (3), 725 - 729, English

  [Refereed]

  Scientific journal


• Analysis of T-cell receptor V beta gene from infiltrating T cells in insulitis and myocarditis in encephalomyocarditis virus-infected BALB/C mice

  A Kawagishi, A Kubosaki, N Takeyama, A Sakudo, K Saeki, Y Matsumoto, T Hayashi, T Onodera

  Oct. 2003, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 310(3) (3), 791 - 795, English

  [Refereed]

  Scientific journal


• Absence of superoxide dismutase activity in a soluble cellular isoform of prion protein produced by baculovirus expression system

  A Sakudo, M Hamaishi, T Hosokawa-Kanai, K Tuchiya, T Nishimura, K Saeki, Y Matsumoto, S Ueda, T Onodera

  Aug. 2003, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 307(3) (3), 678 - 683, English

  [Refereed]

  Scientific journal


• Expression of normal cellular prion protein (PrPc) on T lymphocytes and the effect of copper ion: analysis by wild-type and prion protein gene-deficient mice

  A Kubosaki, Y Nishimura-Nasu, T Nishimura, S Yusa, A Sakudo, K Saeki, Y Matsumoto, S Itohara, T Onodera

  Aug. 2003, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 307(4) (4), 810 - 813, English

  [Refereed]

  Scientific journal


• Impairment of superoxide dismutase activation by N-terminally truncated prion protein (PrP) in PrP-deficient neuronal cell line

  A Sakudo, DC Lee, K Saeki, Y Nakamura, K Inoue, Y Matsumoto, S Itohara, T Onodera

  Aug. 2003, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 308(3) (3), 660 - 667, English

  [Refereed]

  Scientific journal


• 神経内分泌組織におけるIA-2発現解析

  竹山 夏実, 川上 登美子, 佐伯 圭一, 松本 芳嗣, 小野寺 節

  2003, 日仏生物学会誌, 43(1) (1), English

  Symposium


• 動物のプリオン病

  佐伯圭一

  2003, 最新医学, 58(5) (5), 1008 - 1013, Japanese

  [Invited]

  Scientific journal


• スクレイピー (〔2002年〕12月第1土曜特集 プリオン蛋白関連疾患)

  佐伯 圭一, 小野寺 節

  2002.

  Lead, 医歯薬出版, Dec. 2002, 医学のあゆみ, 203(10) (10), 951 - 954, Japanese


• スクレイピー

  佐伯圭一, 小野寺節

  Lead, Oct. 2002, 医学のあゆみ, 203(10) (10), 951 - 954, Japanese

  Scientific journal


• Molecular modulation of expression of prion protein by heat shock

  WC Shyu, HJ Harn, K Saeki, A Kubosaki, Y Matsumoto, T Onodera, CJ Chen, YD Hsu, YH Chiang

  Aug. 2002, MOLECULAR NEUROBIOLOGY, 26(1) (1), 1 - 12, English

  [Refereed]

  Scientific journal


• Targeted disruption of the protein tyrosine phosphatase-like molecule IA-2 results in alterations in glucose tolerance tests and insulin secretion

  K Saeki, M Zhu, A Kubosaki, JP Xie, MS Lan, AL Notkins

  Lead, Jun. 2002, DIABETES, 51(6) (6), 1842 - 1850, English

  [Refereed]

  Scientific journal


• Developmental expression and localization of IA-2 mRNA in mouse neuroendocrine tissues

  S Shimizu, N Saito, A Kubosaki, S SungWook, N Takeyama, T Sakamoto, Y Matsumoto, K Saeki, Y Matsumoto, T Onodera

  Oct. 2001, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 288(1) (1), 165 - 171, English

  [Refereed]

  Scientific journal


• Distribution of Cellular Isoform of Prion Protein in T Lymphocytes and Bone Marrow, Analyzed by Wild-Type and Prion Protein Gene-Deficient Mice

  Atsutaka Kubosaki, Seiichi Yusa, Yukiko Nasu, Takuya Nishimura, Yuko Nakamura, Keiichi Saeki, Yoshitsugu Matsumoto, Shigeyoshi Itohara, Takashi Onodera

  2001, Biochemical and Biophysical Research Communications, 282(1) (1), 103 - 107, English

  Scientific journal


• Comparative analysis of the prion protein open reading frame nucleotide sequences of two wild ruminants, the moufflon and golden takin

  Sung Wook Seo, Kazuhiro Hara, Atsutaka Kubosaki, Yukiko Nasu, Takuya Nishimura, Keiichi Saeki, Yoshitsugu Matsumoto, Hideki Endo, Takashi Onodera

  2001, Intervirology, 44(6) (6), 359 - 363, English

  [Refereed]

  Scientific journal


• Genomic structure of mouse IA-2: comparison with its human homologue

  K Saeki, J Xie, AL Notkins

  Lead, Nov. 2000, DIABETOLOGIA, 43(11) (11), 1429 - 1434, English

  [Refereed]

  Scientific journal


• Analysis of prion protein mRNA by in situ hybridization in brain and placenta of sheep

  A Kubosaki, A Ueno, Y Matsumoto, K Doi, K Saeki, Y Matsumoto, T Onodera

  Jul. 2000, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 273(3) (3), 890 - 893, English

  [Refereed]

  Scientific journal


• Japanese scrapie cases

  T Onodera, K Saeki

  Last, Apr. 2000, JAPANESE JOURNAL OF INFECTIOUS DISEASES, 53(2) (2), 56 - 61, English

  [Refereed]

  Scientific journal


• Enhanced expression of cellular prion protein gene by insulin or nerve growth factor in immortalized mouse neuronal precursor cell lines

  C Kuwahara, A Kubosaki, T Nishimura, Y Nasu, Y Nakamura, K Saeki, Y Matsumoto, T Onodera

  Feb. 2000, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 268(3) (3), 763 - 766, English

  [Refereed]

  Scientific journal


• プリオンレスマウス不死化神経細胞におけるEMCV-Bの増殖

  那須 教子, 西村 拓也, 井上 敬一, 糸原 重美, 佐伯 圭一, 松本 芳嗣, 小野寺 節

  2000, 日仏生物学会誌, 40(1) (1), English

  Symposium


• Prions prevent neuronal cell-line death

  C Kuwahara, AM Takeuchi, T Nishimura, K Haraguchi, A Kubosaki, Y Matsumoto, K Saeki, Y Matsumoto, T Yokoyama, S Itohara, T Onodera

  Jul. 1999, NATURE, 400(6741) (6741), 225 - 226, English

  [Refereed]

  Scientific journal


• The effect of Serum Thymic Factor on TNF-α-induced apoptosis in mouse pancreatic beta cell line(MIN6)

  NISHIOKA Wakako, YASUDA Jyunko, AWAYA Akira, SAEKI Keiichi, MATSUMOTO Yoshinori, ONODERA Takashi

  1999, 日仏生物学会誌, 39(1) (1), 10 - 10, English

  [Refereed]

  Symposium


• Difference in Bgp-independent fusion activity among mouse hepatitis viruses

  F Taguchi, S Matsuyama, K Saeki

  Last, 1999, ARCHIVES OF VIROLOGY, 144(10) (10), 2041 - 2049, English

  [Refereed]

  Scientific journal


• Expression of ovine PrPc mRNA by in situ hybridization.

  Kubosaki A, Kuwahara C, Saeki K, Matsumoto Y, Onodera T

  1998, Anim. Biochem, 35, 19 - 23, Japanese

  Scientific journal


• Differential receptor-functionality of the two distinct receptor proteins for mouse hepatitis virus

  N Ohtsuka, YK Yamada, K Saeki, F Taguchi

  1998, CORONAVIRUSES AND ARTERIVIRUSES, 440, 77 - 80, English

  Scientific journal


• Isolation and characterization of murine coronavirus mutants resistant to neutralization by soluble receptors

  K Saeki, N Ohtsuka, F Taguchi

  Lead, 1998, CORONAVIRUSES AND ARTERIVIRUSES, 440, 11 - 16, English

  Scientific journal


• Identification of spike protein residues of murine coronavirus responsible for receptor-binding activity by use of soluble receptor-resistant mutants

  K Saeki, N Ohtsuka, F Taguchi

  Lead, Dec. 1997, JOURNAL OF VIROLOGY, 71(12) (12), 9024 - 9031, English

  [Refereed]

  Scientific journal


• Epidemiological analysis of ovine scrapie in Japan.

  Onodera T, Saeki, K

  Last, 1997, Bull. Soc. Franco-Japonais Sci. Vet., 8(1) (1), 3 - 7, English

  International conference proceedings


• Identification of a promoter region in the rat prion protein gene

  K Saeki, Y Matsumoto, Y Matsumoto, T Onodera

  Lead, Feb. 1996, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 219(1) (1), 47 - 52, English

  [Refereed]

  Scientific journal


• Three-exon structure of the gene encoding the rat prion protein and its expression in tissues

  K Saeki, Y Matsumoto, Y Hirota, Y Matsumoto, T Onodera

  Lead, 1996, VIRUS GENES, 12(1) (1), 15 - 20, English

  [Refereed]

  Scientific journal


• Analysis of PrPc mRNA by in situ hybridization in brain, placenta, uterus and testis of rats

  K Tanji, K Saeki, Y Matsumoto, M Takeda, K Hirasawa, K Doi, Y Matsumoto, T Onodera

  Nov. 1995, INTERVIROLOGY, 38(6) (6), 309 - 315, English

  [Refereed]

  Scientific journal


• ESTABLISHMENT OF HIPPOCAMPAL CELL-LINES OF MICE HOMOZYGOUS FOR TARGETED DELETION OF THE PRN-P GENE

  AM TAKEUCHI, K SAEKI, Y MATSUMOTO, Y MATSUMOTO, K DOI, T YOKOYAMA, S ITOHARA, T ONODERA

  1995, JOURNAL OF NEUROCHEMISTRY, 65, S101 - S101, English

  International conference proceedings


• Molecular cloning of the prion protein cDNA and its gene expression in rat brain and immortalized neuronal cells.

  Saeki K, Takeuchi AM, Matsumoto Y, Hirota Y, Tabira T, Matsumoto Y, Onodera T

  Lead, 1994, Anim. Biochem, 31, 37 - 42, Japanese

  Scientific journal


• FUSOGENIC PROPERTIES OF UNCLEAVED SPIKE PROTEIN OF MURINE CORONAVIRUS JHMV

  F TAGUCHI, T IKEDA, K SAEKI, H KUBO, T KIKUCHI

  1993, CORONAVIRUSES, 342, 171 - 175, English

  International conference proceedings

• 日本国内で発見されたダニ媒介性オルビウイルス、ムコウイルスのリバースジェネティクス系の樹立

  松尾 栄子, 若村 佳樹, 満田 優希, 柳本 周, 林 昌宏, 佐伯 圭一, 江尻 寛子, 伊藤 睦代[高山], 伊澤 晴彦, 西條 政幸, 沢辺 京子, 河野 潤一

  (公社)日本獣医学会, Aug. 2019, 日本獣医学会学術集会講演要旨集, 162回, 401 - 401, Japanese


• テネイシンC欠損がおよぼすプリオン蛋白遺伝子発現への影響

  烏雲達来, 中山 翔, 松尾 栄子, 河野 潤一, 山崎 智広, 中田 大介, 金 聖大, 紅林 淳一, 礒西 成治, 日下部 守昭, 佐伯 圭一

  (公社)日本獣医学会, Aug. 2012, 日本獣医学会学術集会講演要旨集, 154回, 254 - 254, Japanese


• ZchI型(FVB/Prnp-/-)不死化神経細胞株の樹立および,PrPCのアポトーシス抑制能の解析

  中村 出, 西村 拓也, 李 得燦, 作道 章一, 佐伯 圭一, 松本 芳嗣, 小野寺 節

  (公社)日本獣医学会, Mar. 2004, 日本獣医学会学術集会講演要旨集, 137回, 98 - 98, Japanese


• 正常型プリオン蛋白質による細胞内銅濃度維持

  谷内洋次郎, 作道章一, 李得燦, 吉村悦郎, 長坂征治, 新田佳也子, 佐伯圭一, 糸原重美, 松本芳嗣

  (公社)日本獣医学会, Mar. 2004, 日本獣医学会学術集会講演要旨集, 137回, 98 - 98, Japanese


• Identification of domains important for anti-apoptotic function of cellular prion protein

  佐伯圭一, 作道章一, 小野寺節

  2004, プリオン病及び遅発性ウイルス感染に関する調査研究 平成15年度研究報告書, 158 - 162, Japanese


• 欠損変異プリオン蛋白を用いた正常型プリオン蛋白の機能解析

  李得燦, 作道章一, 西村拓也, 徐聖旭, 佐伯圭一, 松本芳嗣, 小野寺節

  (公社)日本獣医学会, Mar. 2003, 日本獣医学会学術集会講演要旨集, 135回, 132 - 132, Japanese


• アポトーシス抑制因子Bcl-2 family,Superoxide dismutase(SOD)遺伝子及びプリオン遺伝子の導入による不死化プリオンレス神経細胞死の解析

  作道 章一, 李 得燦, 佐伯 圭一, 糸原 重美, 松本 芳嗣, 小野寺 節

  (公社)日本獣医学会, Mar. 2002, 日本獣医学会学術集会講演要旨集, 133回, 100 - 100, Japanese


• プリオン研究の進展 アポトーシス抑制因子Bcl-2 family遺伝子の導入による不死化プリオンレス神経細胞死の解析

  作道 章一, 李 得燦, 佐伯 圭一, 糸原 重美, 松本 芳嗣, 小野寺 節

  (公社)日本獣医学会, Sep. 2001, 日本獣医学会学術集会講演要旨集, 132回, 51 - 51, Japanese


• アポトーシス抑制因子Bcl‐2,Bcl‐xL,Superoxide dismutase(SOD)遺伝子の導入による不死化プリオンレス神経細胞死の解析

  作道章一, 中村優子, 佐伯圭一, 糸原重美, 松本芳嗣, 小野寺節

  (公社)日本獣医学会, Mar. 2001, 日本獣医学会学術集会講演要旨集, 131回, 76 - 76, Japanese


• プリオン蛋白質(PrP)遺伝子欠損マウス及び神経細胞株におけるPrP様蛋白質(Dpl)遺伝子の発現

  佐伯 圭一, 作道 章一, 中村 優子, 那須 教子, 糸原 重美, 松本 芳嗣, 小野寺 節

  (公社)日本獣医学会, Mar. 2001, 日本獣医学会学術集会講演要旨集, 131回, 76 - 76, Japanese


• リーシュマニア原虫を用いた哺乳動物遺伝子発現系の検討

  中村 優子, 畑生 俊光, 松本 安喜, 河津 信一郎, Chang K.-P, 佐伯 圭一, 小野寺 節, 松本 芳嗣

  (公社)日本獣医学会, Mar. 1999, 日本獣医学会学術集会講演要旨集, 127回, 93 - 93, Japanese

• 今日から「菌トレ」！ オソロしくてオモロい、菌とのくらし 中国語版

  MIYAKO AKIKO, SAEKI KEIICHI

  Supervisor, 聯經出版公司, Apr. 2015, Chinese, 今天開始練菌！：與可怕又有趣的好菌壞菌和平相處

  General book


• 今日から「菌トレ」！～オソロしくてオモロい、菌とのくらし～

  MIYAKO AKIKO, SAEKI KEIICHI

  Supervisor, 小学館, Jul. 2013, Japanese, ISBN: 9784093883108

  General book


• 生体防御医学事典

  SUZUKI KAZUO, SAEKI KEIICHI

  Others, Asakura Publishing Co. Ltd., May 2007, Japanese

  Scholarly book


• 脳とプリオン―狂牛病の分子生物学 (シリーズ・応用動物科学バイオサイエンス)

  ONODERA TAKASHI, SAEKI KEIICHI

  Joint work, 朝倉書店, Sep. 2001, Japanese

  General book


• 農学21世紀への挑戦～地球を救う50の提案～

  SAEKI KEIICHI, 東京大学大学院農学生命科学研究科編著

  Others, 世界文化社, Mar. 2000, Japanese

  General book

• ネオニコチノイド系農薬クロチアニジンの胎子・授乳期曝露による次世代マウスの免疫系および腸内細菌叢の変化

  村田 碧, 西 美咲, 正田 明日香, 池中 良徳, 佐伯 圭一, 松尾 栄子, 平野 哲史, 万谷 洋平, 横山 俊史, 星 信彦

  環境ホルモン学会研究発表会要旨集, Sep. 2021, Japanese, 日本内分泌撹乱物質学会


• ネオニコチノイド系農薬クロチアニジンの胎子・授乳期曝露が次世代マウスの免疫系および腸内細菌叢に及ぼす影響

  村田 碧, 西 美咲, 正田 明日香, 佐伯 圭一, 松尾 栄子, 万谷 洋平, 横山 俊史, 平野 哲史, 池中 良徳, 星 信彦

  日本獣医学会学術集会講演要旨集, Sep. 2021, Japanese, (公社)日本獣医学会


• The influence of the transcriptional regulatory motifs within the 5′ upstream region of mouse prion protein gene on the transcriptional activity.

  Inoue S, Kato R, Matsuo E, Kawano J, Saeki K

  International Union of Microbiological Societies (IUMS), Nov. 2020, English

  Oral presentation


• マウスプリオン蛋白質遺伝子エクソン１上流域内のHES-1結合配列が遺伝子発現に及ぼす影響

  井上創太, 加藤亮, 松尾栄子, 佐伯圭一

  第73回細菌学会関西支部総会, Nov. 2020, Japanese

  Oral presentation


• オルビウイルス複製におけるタンパク質翻訳機構の解析

  杉本昌寛, 松田梢, 満田優希, 佐伯圭一, 河野潤一, 松尾栄子

  第73回細菌学会関西支部総会, Nov. 2020, Japanese

  Oral presentation


• オルビウイルス構造タンパク質VP6の機能解析

  満田優希, 若村佳樹, 松田梢, 佐伯圭一, 河野潤一, 松尾栄子

  第73回細菌学会関西支部総会, Nov. 2020, Japanese

  Oral presentation


• 流行性出血病（イバラキ病様疾病）発生牧場におけるウイルス中和抗体の追跡調査

  松田梢, 深沢太一, 満田優希, 佐伯圭一, 河野潤一, 松尾栄子

  第73回細菌学会関西支部総会, Nov. 2020, Japanese

  Oral presentation


• マウスプリオン蛋白質遺伝子エクソン１上流域における転写制御領域の解析

  井上創太, 加藤亮, 七森和, 寺前初音, 木村将徳, 藤山諒太, 松尾栄子, 佐伯圭一

  第163回日本獣医学会学術集会, Sep. 2020, Japanese, DVO-49(井上).pdf, Published, No password

  Oral presentation

  添付ファイルのURL


• CCAATエンハンサー結合蛋白質ファミリーによるプリオン蛋白質 遺伝子発現への影響

  木村 将徳, 井上 創太, 加藤 亮, 七森 和, 寺前 初音, 藤山 諒太, 松尾 栄子, 佐伯 圭一

  第163回日本獣医学会学術集会, Sep. 2020, Japanese, DVO-52（藤山）.pdf, No password

  添付ファイルのURL


• ニホンジカプリオン蛋白質遺伝子における翻訳領域とプロモーター領域に関する研究

  藤山 諒太, 加藤 亮, 七森 和, 井上 創太, 寺前 初音, 木村 将徳, 松尾 栄子, 佐伯 圭一

  第163回日本獣医学会学術集会, Sep. 2020, Japanese, DVO-52（藤山）.pdf, No password

  Oral presentation

  添付ファイルのURL


• プリオン蛋白質のα開裂に関する研究

  寺前 初音, 七森 和, 井上 創太, 加藤 亮, 木村 将徳, 藤山 諒太, 松尾 栄子, 佐伯 圭一

  第163回日本獣医学会学術集会, Sep. 2020, Japanese, DVO-53（寺前）.pdf, No password

  Oral presentation

  添付ファイルのURL


• 流行性出血病（イバラキ病様疾病）発生牧場におけるウイルス中和抗体の追跡調査

  松田 梢, 深沢 太一, 佐伯 圭一, 松尾 栄子

  第163回日本獣医学会学術集会, Sep. 2020, Japanese

  Oral presentation


• 細胞型プリオン蛋白質のα開裂に関する研究

  七森和、加藤亮、井上創太、松尾栄子、佐伯圭一

  神戸大学研究基盤センター若手フロンティア研究会, Dec. 2019, Japanese

  Poster presentation


• 日本国内で発見されたダニ媒介性オルビウイルス、ムコウイルスのリバースジェネティクス系の樹立

  松尾栄子、柳本周、満田優希、若村佳樹、林昌宏、佐伯圭一、江尻寛子、伊藤(高山)睦代、伊澤晴彦、西條政幸、沢辺京子、河野潤一

  第72回 日本細菌学会関西支部総会, Nov. 2019, Japanese

  Oral presentation


• Fuzzy self-recognition mechanisms of orbivirus during core assembly in virus inclusion body

  Eiko Matsuo, Adeline Kerviel, Yuki Mitsuda, Chang-Kweng Lim, Keiichi Saeki, Masayuki Saijo, Junichi Kawano, Polly Roy

  第67回日本ウイルス学会学術集会, Oct. 2019, Japanese


• 日本国内で発見されたダニ媒介性オルビウイルス、ムコウイルスのリバースジェネティクス系の樹立

  松尾栄子、若村佳樹、柳本周、満田優希、林昌宏、佐伯圭一、江尻寛子、伊藤(高山)睦代、伊澤晴彦、西條政幸、沢辺京子、河野潤一

  第162回日本獣医学会学術集会, Sep. 2019, Japanese

  Oral presentation


• 日本国内で発見されたダニ媒介性オルビウイルス,ムコウイルスのリバースジェネティクス系の樹立

  松尾栄子, 若村佳樹, 満田優希, 柳本周, 林昌宏, 佐伯圭一, 江尻寛子, 伊藤(高山)睦代, 伊澤晴彦, 西條政幸, 沢辺京子, 河野潤一

  日本獣医学会学術集会講演要旨集, 2019


• 新規遺伝子組換え技術を用いた流行性出血病ウイルス各血清型に対する迅速な中和抗 体検出法の開発

  MATSUO EIKO, Fukazawa Taichi, SAEKI KEIICHI, KAWANO JUNICHI

  第71回日本細菌学会関西支部総会, Oct. 2018, Japanese, 大阪大学中之島センター10F 佐治敬三メモリアルホール, Domestic conference

  Oral presentation


• Visualization of Epizootic hemorrhagic Disease Virus Entry and Protein Synthesis

  MATSUO EIKO, Hiroko Omori, SAEKI KEIICHI, KAWANO JUNICHI

  The 66th Annual meeting of the Japanese Society for Virology, Oct. 2018, English, 京都テルサ, Domestic conference

  Oral presentation


• 新規遺伝子組換え技術を用いた流行性出血病ウイルス各血清型に対する迅速な中和抗 体検出法の開発

  MATSUO EIKO, Fukazawa Taichi, SAEKI KEIICHI, KAWANO JUNICHI

  第161回 日本獣医学会学術集会, Sep. 2018, Japanese, つくば国際会議場, Domestic conference

  Oral presentation


• Further analysis of Ibaraki virus VP6 to produce fluorescence-labeled orbiviruses

  MATSUO EIKO, Marina Hamaji, Hiroko Omori, Tsuji Hideyuki, Akari Saito, Polly Roy, SAEKI KEIICHI, Takeshi Kobayashi, KAWANO JUNICHI

  13th International dsRNA Virus Symposium, Sep. 2018, English, Houffalize, Belgium, International conference

  Poster presentation


• マウス肝細胞におけるイバラキウイルス（IBAV）増殖抑制に関する研究

  濱治 麻理奈, SAEKI KEIICHI, KAWANO JUNICHI, MATSUO EIKO

  手フロンティア研究会２０１７, Dec. 2017, Japanese, 神戸大学百年記念館, Domestic conference

  Poster presentation


• オルビウイルス構造タンパク質VP6の細胞内局在とgenome packaging機能に関する研究

  MATSUO EIKO, SAEKI KEIICHI, KAWANO JUNICHI, ROY POLLY

  第70回細菌学会関西支部総会, Nov. 2017, Japanese, 大阪府立大学 I-siteなんば 2F, Domestic conference

  Oral presentation


• マウス肝細胞におけるイバラキウイルス（IBAV）増殖抑制に関する研究

  濱治 麻理奈, SAEKI KEIICHI, KAWANO JUNICHI, MATSUO EIKO

  第65回日本ウイルス学会学術集会, Oct. 2017, Japanese, 大阪国際会議場, Domestic conference

  Poster presentation


• Biological roles of a loop region of orbivirus VP6 in virus replication

  MATSUO EIKO, SAEKI KEIICHI, KAWANO JUNICHI, Roy Polly

  The 65th Annual meeting of the Japanese Society for Virology, Oct. 2017, English, 大阪国際会議場, Domestic conference

  Oral presentation


• Enhancement of Ibaraki virus replication by a viral non-structural protein NS2

  saji tomoaki, hirano junki, SAEKI KEIICHI, KAWANO JUNICHI, MATSUO EIKO

  第4回 関西ウイルスクラブ（KVC）, Feb. 2017, Japanese, 大阪医科大学, Domestic conference

  [Invited]

  Nominated symposium


• イバラキウイルスの構造タンパク質VP3の機能解析

  sakura risa, SAEKI KEIICHI, KAWANO JUNICHI, MATSUO EIKO

  若手フロンティア研究会２０１６, Dec. 2016, Japanese, 神戸大学百年記念館, Domestic conference

  Oral presentation


• 蛍光標識イバラキウイルス粒子を用いた新規cell binding assay系の確立

  hamaji marina, SAEKI KEIICHI, KAWANO JUNICHI, MATSUO EIKO

  第69回 日本細菌学会関西支部総会, Nov. 2016, Japanese, 大阪市立大学 杉本キャンパス 田中記念館, Domestic conference

  Oral presentation


• オルビウイルス構造タンパク質VP6の機能解析

  MATSUO EIKO, SAEKI KEIICHI, KAWANO JUNICHI, Roy Polly

  第69回 日本細菌学会関西支部総会, Nov. 2016, Japanese, 大阪市立大学 杉本キャンパス 田中記念館, Domestic conference

  Oral presentation


• イバラキウイルス非構造タンパク質NS2のウイルス複製促進作用に関する研究

  saji tomoaki, hirano junki, SAEKI KEIICHI, KAWANO JUNICHI, MATSUO EIKO

  第69回 日本細菌学会関西支部総会, Nov. 2016, Japanese, 大阪市立大学 杉本キャンパス 田中記念館, Domestic conference

  Oral presentation


• Further analysis of Ibaraki virus VP6 to produce fluorescence-labeled orbiviruses

  MATSUO EIKO, HAMAJI MARINA, SAITO AKARI, SAEKI KEIICHI, KAWANO JUNICHI

  The 64th Annual meeting of the Japanese Society for Virology, Oct. 2016, English, 札幌コンベンションセンター, Domestic conference

  Oral presentation


• Reverse Genetics Systemを用いたイバラキウイルス粒子形成機序の解析

  MATSUO EIKO, SAEKI KEIICHI, KAWANO JUNICHI

  第68回 日本細菌学会関西支部総会, Nov. 2015, Japanese, 京都薬科大学 愛学館, Domestic conference

  Oral presentation


• Reverse genetics system for Ibaraki virus reveals the essential mechanisms of VP6 in its replication

  MATSUO EIKO, SAEKI KEIICHI, KAWANO JUNICHI

  The 63rd Annual meeting of the Japanese Society for Virology, Nov. 2015, English, 福岡国際会議場, Domestic conference

  Oral presentation


• Development of Reverse Genetics for Ibaraki Virus to Produce a Viable VP6-Tagged IBAV

  MATSUO EIKO, SAEKI KEIICHI, Roy Polly, KAWANO JUNICHI

  The 12th International Double Stranded RNA Virus Symposium, Oct. 2015, English, Goa Marriott Resort & Spa, Goa, India, International conference

  Oral presentation


• Mechanisms of Resistance to Ibaraki Virus Replication and Its Persistent Infection in a Murine Hepatocyte Cell Line

  MATSUO EIKO, IWATA YUI, HAMAJI MARINA, SAEKI KEIICHI, KAWANO JUNICHI

  The 14th Awaji International Forum on Infection and Immunity, Sep. 2015, English, Awaji Yumebutai International Conference Center, International conference

  Poster presentation


• Development of reverse genetics for Ibaraki virus to produce viable VP6-tagged IBAV

  MATSUO EIKO, SAEKI KEIICHI, Roy Polly, KAWANO JUNICHI

  Eighth International Virus Assembly Symposium, May 2015, English, Dubrovnik, Croatia, International conference

  Poster presentation


• マウス肝細胞におけるイバラキウイルスの持続感染について

  Yui Iwata, Risa Sakura, Shoko Nishimura, Junki Hirano, Yu Murakami, Keiichi Saeki, Junichi, MATSUO EIKO

  第６７回日本細菌学会関西支部総会・学術集会, Nov. 2014, Japanese, 兵庫医科大学 ３-３講義室（３号館４階）, Domestic conference

  Oral presentation


• イバラキウイルス（IBAV）における新規遺伝子改変法（Reverse Genetics System） を用いた、VP6 変異IBAV の作出

  MATSUO EIKO, Keiichi Saeki, Junichi

  第６７回日本細菌学会関西支部総会・学術集会, Nov. 2014, Japanese, 兵庫医科大学 ３-３講義室（３号館４階）, Domestic conference

  Oral presentation


• イバラキウイルス（IBAV）におけるReverse Genetics Systemの開発とその応用

  MATSUO EIKO, Keiichi Saeki, Junichi

  第62回日本ウイルス学会学術集会, Nov. 2014, Japanese, パシフィコ横浜 会議センター, Domestic conference

  Oral presentation


• イバラキウイルス(IBAV)コアタンパク質VP6 とVP3 の相互作用に関する研究

  Yu Murakami, Hideyuki Tsuji, Risa Sakura, Shoko Nishimura, Junki Hirano, Keiichi Saeki, Junichi, MATSUO EIKO

  第６７回日本細菌学会関西支部総会・学術集会, Nov. 2014, Japanese, 兵庫医科大学 ３-３講義室（３号館４階）, Domestic conference

  Oral presentation


• 広溶菌域ブドウ球菌ファージに関する研究

  Masashi Kishida, Shinichiro Tobi, Yuriko Sugimoto, MATSUO EIKO, Junichi, Keiichi Saeki

  第157回日本獣医学会学術集会, Sep. 2014, Japanese, 北海道大学高等教育推進機構, Domestic conference

  Oral presentation


• マウス由来株化培養細胞におけるプリオン蛋白遺伝子の発現とDNAメチル化状態

  烏雲達来, Yugo Doi, Natsumi Takeyama, MATSUO EIKO, Junichi, Keiichi Saeki

  第157回日本獣医学会学術集会, Sep. 2014, Japanese, 北海道大学高等教育推進機構, Domestic conference

  Oral presentation


• マウス肝細胞におけるイバラキウイルスの持続感染について

  Yui Iwata, Risa Sakura, Shoko Nishimura, Junki Hirano, Yu Murakami, Keiichi Saeki, Junichi, MATSUO EIKO

  第157回日本獣医学会学術集会, Sep. 2014, Japanese, 北海道大学高等教育推進機構, Domestic conference

  Oral presentation


• ブルータングウイルス（BTV）VP6の構造解析を用いた増殖可能なVP6-truncated BTVの作出

  MATSUO EIKO, Esther Leon, Keiichi Saeki, Junichi Kawano, Steve Matthews, Polly Roy

  第157回日本獣医学会学術集会, Sep. 2014, Japanese, 北海道大学高等教育推進機構, Domestic conference

  Oral presentation


• The essential interaction of VP6 protein with VP3 for recruitment of the replicase complex into orbivirus particle

  MATSUO EIKO, Hideyuki Tsuji, Yu Murakami, Keiichi Saeki, Junichi Kawano, Polly Roy

  The 13th Awaji International Forum on Infection and Immunity in Nara, Sep. 2014, English, Nara Prefectural New Public Hall, International conference

  Oral presentation


• 腫瘍におけるプリオン蛋白遺伝子発現は間質由来テネイシンCによって調整されている(Stromal tenascin C regulates a prion protein gene expression in tumor tissues)

  佐伯 圭一, オヨン・ダライ, 松尾 栄子, 河野 潤一, 日下部 美帆, 日下部 守昭

  日本癌学会総会記事, Oct. 2013, English, (一社)日本癌学会


• Stromal tenascin C regulates a prion protein gene expression in tumor tissues.

  SAEKI KEIICHI, Wuyundarai, MATSUO EIKO, KAWANO JUNICHI, Kusakabe Miho, KUSAKABE MORIAKI

  The 72nd Annual Meeting of the Japanese Cancer Association, Oct. 2013, Japanese, PACIFICO YOKOHAMA, Domestic conference

  Poster presentation


• マウスプリオン蛋白遺伝子発現制御領域のメチル化に関する研究

  Wuyundarai, TAKEYAMA NATSUMI, KUROKAWA TAKURO, DOI YUGO, MATSUO EIKO, KAWANO JUNICHI, SAEKI KEIICHI

  156th Meeting of the Japanese Society of Veterinary Science, Sep. 2013, Japanese, GIFU UNIVERSITY, Domestic conference

  Oral presentation


• 生体内における感染動態解析のためのEGFP発現大腸菌の作製

  SAKAMOTO NATSUMI, MATSUO EIKO, SAEKI KEIICHI, KAWANO JUNICHI

  第155回日本獣医学会, Mar. 2013, Japanese, 日本獣医学会, 東京, Domestic conference

  Poster presentation


• 異なる細胞種におけるイバラキウイルスの複製能力の比較

  岩田 友伊, 辻 秀幸, SAEKI KEIICHI, KAWANO JUNICHI, MATSUO EIKO

  第155回日本獣医学会学術集会, Mar. 2013, Japanese, 東京, Domestic conference

  Oral presentation


• オルビウイルスコアタンパク質VP6とVP3の相互作用に関する研究

  辻 秀幸, 岩田 友伊, SAEKI KEIICHI, KAWANO JUNICHI, Polly Roy, MATSUO EIKO

  第155回日本獣医学会学術集会, Mar. 2013, Japanese, 東京, Domestic conference

  Oral presentation


• ブルータングウイルス（BTV）コアタンパク質VP3とVP6の結合に関する研究

  MATSUO EIKO, 山崎 清志, Esther Leon, SAEKI KEIICHI, KAWANO JUNICHI, Steve Matthew, TSURUTA HIROKI, Polly Roy

  第65回日本細菌学会関西支部総会, Nov. 2012, Japanese, 神戸, Domestic conference

  Oral presentation


• ブルータングウイルス（BTV）コアタンパク質VP3とVP6の結合に関する研究

  MATSUO EIKO, Esther Leon, 山崎 清志, SAEKI KEIICHI, KAWANO JUNICHI, Steve Matthew, TSURUTA HIROKI, Polly Roy

  第60回日本ウイルス学会学術集会, Nov. 2012, Japanese, 大阪, Domestic conference

  Oral presentation


• ブルータングウイルス（BTV）コアタンパク質VP3とVP6の結合に関する研究

  MATSUO EIKO, Esther Leon, SAEKI KEIICHI, KAWANO JUNICHI, Steve Matthew, Polly Roy

  第154回日本獣医学会学術集会, Sep. 2012, Japanese, 盛岡, Domestic conference

  Oral presentation


• テネイシンC欠損がおよぼすプリオン蛋白遺伝子発現への影響

  Wuyundarai, NAKAYAMA SHO, MATSUO EIKO, KAWANO JUNICHI, YAMAZAKI TOMOHIRO, NAKATA DAISUKE, KIMU SONDE, KUREBAYASHI JUNICHI, ISONISHI NEIHARU, KUSAKABE MORIAKI, SAEKI KEIICHI

  154th Meeting of the Japanese Society of Veterinary Science, Sep. 2012, Japanese, IWATE UNIVERSITY, Domestic conference

  Oral presentation


• テネイシンC欠損がおよぼすプリオン蛋白遺伝子発現への影響

  烏雲達来, 中山 翔, 松尾 栄子, 河野 潤一, 山崎 智広, 中田 大介, 金 聖大, 紅林 淳一, 礒西 成治, 日下部 守昭, 佐伯 圭一

  日本獣医学会学術集会講演要旨集, Aug. 2012, Japanese, (公社)日本獣医学会


• 複製能力欠損ブルータングウイルスを用いた新規ワクチンの開発

  MATSUO EIKO, Cristina CP Celma, Richard Thiery, SAEKI KEIICHI, KAWANO JUNICHI, Stephan Zientara, Polly Roy

  第153回日本獣医学会学術集会, Mar. 2012, Japanese, 大宮ソニックシティ, Domestic conference

  Poster presentation


• 新規MRSA分離選択培地の開発

  KAKITA YOKO, SHIMODA HIROYUKI, SHIMABUKURO SHIZUKA, SAITO ETSUKO, SAEKI KEIICHI, KAWANO JUNICHI

  150th Meeting of the Japanese Society of Veterinary Science, Sep. 2010, Japanese, OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE, Domestic conference

  Oral presentation


• 腫瘍組織におけるプリオン蛋白（PrP）遺伝子発現量の比較

  YAMAZAKI TOMOHIRO, MIYAGAWA MOMOKO, FUJITA KOUSUKE, KAWANO JUNICHI, KUREBAYASHI JUNICHI, ISHIKAWA HIROSHI, KUSAKABE MORIAKI, SAEKI KEIICHI

  150th Meeting of the Japanese Society of Veterinary Science, Sep. 2010, Japanese, OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE, Domestic conference

  Oral presentation


• 乳飲みマウスにおける微粒子の腸上皮への取込み

  阿野泰久, 山内啓史, 佐伯圭一, 松本芳嗣, 中山裕之, 小野寺節

  日本獣医学会学術集会講演要旨集, Mar. 2007, Japanese


• Expression of hamster PrP and bovine PrP can not prevent murine prion protein gene-deficient cells from apoptosis

  WU Guoying, SAKUDO Akikazu, SAEKI Keiichi, MATSUMOTO Yoshitsugu, ONODERA Takashi

  日本生体防御学会学術総会講演抄録集, 2006, English


• 欠損変異プリオン蛋白質の作成によるプリオン蛋白機能解明の試み

  作道章一, 李樹明, 李得燦, 西村拓也, 佐伯圭一, 松本芳嗣, 糸原重美, 辻祥太郎, 小野寺節, 生田和良

  日本ウイルス学会学術集会プログラム・抄録集, Nov. 2004, Japanese


• プリオン遺伝子欠損神経細胞株におけるアポトーシス経路の解析

  谷内安規子, 西村拓也, 作道章一, 李得燦, 中村出, 松本芳嗣, 佐伯圭一, 糸原重美, 小野寺節

  日本ウイルス学会学術集会プログラム・抄録集, Nov. 2004, Japanese


• 正常型プリオン蛋白質による細胞内銅濃度維持

  谷内洋次郎, 作道章一, 李得燦, 吉村悦郎, 長坂征治, 新田佳也子, 佐伯圭一, 糸原重美, 松本芳嗣

  日本獣医学会学術集会講演要旨集, Mar. 2004, Japanese, (公社)日本獣医学会


• ZchI型(FVB/Prnp^-/-)不死化神経細胞株の樹立および,PrP^Cのアポトーシス抑制能の解析

  中村出, 西村拓也, 李得燦, 作道章一, 佐伯圭一, 松本芳嗣, 小野寺節

  日本獣医学会学術集会講演要旨集, Mar. 2004, Japanese, (公社)日本獣医学会


• 欠損変異プリオン蛋白を用いた正常型プリオン蛋白の機能解析

  李得燦, 作道章一, 西村拓也, 金芝けい, 佐伯圭一, 松本芳嗣, 小野寺節

  日本生体防御学会学術総会講演抄録集, Jul. 2003, Japanese


• 欠損変異プリオン蛋白を用いた正常型プリオン蛋白の機能解析

  李得燦, 作道章一, 西村拓也, 徐聖旭, 佐伯圭一, 松本芳嗣, 小野寺節

  日本獣医学会学術集会講演要旨集, Mar. 2003, Japanese, (公社)日本獣医学会


• アポトーシス抑制因子Bcl‐2 family,Superoxide dismutase(SOD)遺伝子およびプリオン遺伝子の導入による不死化プリオンレス神経細胞死の解析

  作道章一, 李得燦, 佐伯圭一, 糸原重美, 松本芳嗣, 小野寺節

  日本獣医学会学術集会講演要旨集, Mar. 2002, Japanese, (公社)日本獣医学会


• アポトーシス抑制因子Bcl‐2 family遺伝子の導入による不死化プリオンレス神経細胞死の解析

  作道章一, 李得燦, 佐伯圭一, 糸原重美, 松本芳嗣, 小野寺節

  日本獣医学会学術集会講演要旨集, Sep. 2001, Japanese, (公社)日本獣医学会


• プリオン蛋白質(PrP)遺伝子欠損マウスおよび神経細胞株におけるPrP様蛋白質(Dpl)遺伝子の発現

  佐伯圭一, 作道章一, 中村優子, 那須教子, 糸原重美, 松本芳嗣, 小野寺節

  日本獣医学会学術集会講演要旨集, Mar. 2001, Japanese


• アポトーシス抑制因子Bcl‐2,Bcl‐xL,Superoxide dismutase(SOD)遺伝子の導入による不死化プリオンレス神経細胞死の解析

  作道章一, 中村優子, 佐伯圭一, 糸原重美, 松本芳嗣, 小野寺節

  日本獣医学会学術集会講演要旨集, Mar. 2001, Japanese, (公社)日本獣医学会


• プリオン蛋白質(PrP)遺伝子ノックアウト神経細胞株におけるPrP様蛋白質(Dpl)遺伝子(Prnd)の発現

  佐伯圭一, 作道章一, 中村優子, 那須教子, 糸原重美, 松本芳嗣, 小野寺節

  日本分子生物学会年会プログラム・講演要旨集, Nov. 2000, Japanese


• アポトーシス抑制因子Bcl‐2による不死化プリオンレス神経細胞死の抑制

  原口景子, 作道章一, 窪崎敦隆, 桑原千恵子, 西村拓也, 糸原重美, 佐伯圭一, 松本芳嗣, 小野寺節

  日本分子生物学会年会プログラム・講演要旨集, Nov. 1999, Japanese


• リーシュマニア原虫を用いた哺乳動物遺伝子発現系の検討

  中村 優子, 畑生 俊光, 松本 安喜, 河津 信一郎, Chang K.-P, 佐伯 圭一, 小野寺 節, 松本 芳嗣

  日本獣医学会学術集会講演要旨集, Mar. 1999, Japanese, (公社)日本獣医学会

• 日本獣医学会

• プリオン蛋白遺伝子転写制御領域のエピジェネティクス

  佐伯 圭一

  科学研究費補助金／基盤研究(C), Apr. 2012 - Mar. 2015, Principal investigator

  Competitive research funding


• Study on the prion protein functions and tumor control

  SAEKI Keiichi, TAKEYAMA Natsumi, KUSAKABE Moriaki

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 2009 - 2011, Principal investigator

  To understand the physiological function of prion protein(PrP), the expression level of PrP gene was investigated using various tumors transplanted in the nude mouse and tenascin C deficient nude mouse, respectively. PrP gene expression levels were varied in the implanted cancer cells. This study provided the information necessary to elucidate a link between PrP function and cancer cell surviving.

  Competitive research funding


• Pathogenesis of BSE and highly sensitive isolation of BSE agent

  ONODERA TAKASBI, MATSUMOTO Yoshitsugu, SAEKI KEIICHI

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), The University of Tokyo, 2005 - 2007

  The chain reaction of BSE epidemics in the UK and Europe and subsequent emergence of vCJD in young adults and teenagers have raised concerns and highlighted the importance of risk assessment in the food chain. Recently, several highly sensitive methods for detecting priors have been developed. Representative of these is PMCA. Originally developed by Claudio Soto and his colleagues, PMCA has been a hot topic of debate in prion meetings all over the world. A broad spectrum of PrP^Sc species have now been successfully amplified using PMCA, including CWD, mouse-adapted scrapie, and BSE. Studies with human sporadic and variant CJD cases show that PMCA amplification efficiency is tightly controlled by the PrP^C substrate genotype at codon 129. PMCA appears to overcome the species barrier encountered during cross-species transmission more rapidly than in vivo. By "forcing" the technique with lower dilutions of the PrP^Sc seed and more amplification rounds, mouse Chandler PrP^Sc can now convert hamster PrP^C or cervid PrP^C, a conversion that might be observable in vivo but only with extremely long incubation periods. Castilla J. showed that using PMCA, PrP^Sc was generated from the healthy brains of 11 different species, including bank voles, mice, cattle, humans, sheep and rabbits, generating a variety of electrophoretic profiles. PMCA was able to detect PrP^Sc in as little as 1 μL of blood from an asymptomatic prion-infected mouse. Given the increasing evidence of human to human transmission via blood products, scientists are waiting for PMCA to be incorporated into a reliable test with the ability to identify blood donors that are asymptomatic carriers. Further advances in amplification technology are to be expected and the replacement of PrP^C by recombinant PrP as a substrate as well as the use of intermittent shaking rather than sonication should circumvent some of the difficulties in the near future.


• Study for atypical BSE in Italy

  ONODERA Takashi, SAEKI Keiichi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), The University of Tokyo, 2005 - 2006

  A monoclonal antibody (mAb) panel to bovine prion protein (PrP) was studied by immunoblotting and immunohistochemistry for scrapie, and bovine spongiform encephalopathy. The mAb panel is useful because they recognized both normal (PrP^c) and abnormal (PrP^) isoforms of PrP in murine, ovine and bovine brain tissues. Among them, two mAbs recognized a conformational or fragmented PrP epitope. Interestingly, an anti-bovine PrP mAb, 1D12, prepared by immunizing PrP gene-knockout mice with a synthetic polypeptides corresponding to codons 153-166 of the bovine PrP gene showed the novel patterns of reactivity for prion-uninfected neuronal cells. When an enzyme-linked immunosorbent assay-mapping of the mAb epitopes was examined, monoclonal 1D12 reacted with YEDRY and M corresponding to amino acids 156-160 and 165 of bovine PrP. Several patterns of bovine PrP^c distribution in PrP-deficient neuronal cells (HpL3-4) transfected with bovine PrP were observed after different fixation methods. Staining of cell surface was observed after formalin fixation by immunofluorescent assay of 1D12 with confocal microscopy, whereas granules in nucleus were stained after acetone fixation. No reactivity in the nucleus was observed to HpL3-4 or HpL3-4 cells expressing mouse PrP. Reactivity in the nucleus was observed to HpL3-4 cells expressing hamster or bovine PrP. It is the first report to detect the PrPc at both cell surface and nuclei of prion-uninfected cell line. Furthermore, as nuclear PrP^c was specifically recognized by 1D12, amino acids 156-160 and 165 of hamster or bovine PrP may play important role in localization of PrP^c into nucleus.


• 免疫担当細胞におけるプリオン蛋白質の作用機構に関する研究

  佐伯 圭一

  日本学術振興会, 科学研究費助成事業, 若手研究(A), 東京大学, 2002 - 2004

  [プリオン蛋白(PrP)の機能とアポトーシスの抑制に関する解析] Zn-1型PrP遺伝子欠損マウスおよびRikn型PrP遺伝子欠損マウスおよび野生型マウスより得られた神経細胞初代を用いて銅イオンによって誘導されるアポトーシスについて解析した結果、PrP遺伝子欠損マウス由来神経細胞が有為に高いアポトーシスを起こした。 また、Zn-1型PrP遺伝子欠損マウス、Rikn型PrP遺伝子欠損マウスおよび野生型マウスより得られた脾細胞を用いて無刺激および刺激後の細胞の増殖能について比較検討を行った結果、PMA、イオノマイシン刺激後において野生型マウス由来脾細胞と比較してZn-1型PrP遺伝子欠損マウスおよびRikn型PrP遺伝子欠損マウス由来脾細胞において増殖能が低い結果が得られた。ConA、リポ多糖刺激においては野生型マウス由来脾細胞とPrP遺伝子欠損マウス由来脾細胞間で有為な差は認められなかった。アポトーシス細胞についても解析を行ったが有為な差は認められなかった。 [ウイルス感染時におけるプリオン蛋白の機能解析] Zn-1型、Rikn型PrP遺伝子欠損マウスおよび野生型マウスを用いて、脳心筋炎ウイルス-B株感染について解析した結果、アポトーシス細胞の出現頻度においてPrP遺伝子欠損マウスのほうが、野生型マウスと比較して高頻度であった。


• research for highly sensitive assay for BSE agents

  ONODERA Takashi, MATSUMOTO Yoshitsugu, SAEKI Keiichi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), The University of Tokyo, 2002 - 2004

  PrP^c is a host protein anchored to the outer surface of neurons and to a lesser extent in lymphocytes. The transmissible agent (PrP^) responsible for scrapie is thought to be a modified form of PrP^c. However, the role of PrP^c in normal uninfected animals is still unknown. Here we describe a novel mouse model for cardiomyopathy, which was observed during the development of prion agent susceptible transgenic (Tg) mouse. These Tg mice were shown to selectively express OrPrP in their tissues, as demonstrated by RT-PCR for mRNA, Western blotting and immunohistochemistry. High levels of OrPrP expression were seen in heart, medium levels in skeletal muscle, low levels in brain, and barely detectable levels in lung, liver, spleen, kidney and thymus. Interestingly, the electrocardiogram of these mice showed that cardiac muscle contraction was significantly prolonged with abnormality of the QRS interval. In addition, the histologic study showed multiple intracytoplasmic vacuolation in the heart muscle. These data suggest that the high expression of OrPrP in heart may cause the cardiac abnormality. Furthermore, an abnormal waveform of ECG was observed in these Tg mice after atropine injection. These mice therefore may be possible to provide a new model to elucidate experimental cardiomyopathy.


• プリオン蛋白質関連神経細胞死発現機構に関する研究

  佐伯 圭一

  日本学術振興会, 科学研究費助成事業, 特定領域研究, 東京大学, 2002 - 2002

  プリオン蛋白質(PrP)の再発現に伴い細胞のSuperoxide dismutase (SOD)活性を上昇させたとの前年度の結果から、本年度はPrPと酸化ストレスとの関連について調べた。無血清培養条件下におけるPrP欠損神経細胞とPrP再発現細胞株の活性酸素関連分子の量を調べた。SODは2O_2・^-+2H^+→H_2O_2+O_2の反応を触媒する酵素であり、活性酸素(O_2・^-)を消去して過酸化水素(H_2O_2)に変換する。血清除去下での活性酸素の発生量をフリーラジカル特異的蛍光プローブを用いたフローサイトメトリー解析により調べた。Dihydroethidium (DH)により、細胞内活性酸素を蛍光染色しフローサイトメトリーにより測定したところ、PrP欠損神経細胞で見られる活性酸素の発生がPrP再発現細胞株では抑制された。さらに2',7'-dichlorofluorescein diacetate (DCFH-DA)染色により過酸化水素量の測定をおこなったところ、PrP再発現細胞株において過酸化水素の蓄積が認められた。PrP再発現に伴い細胞内の活性酸素が消去され、過酸化水素が蓄積していたことからPrPがSOD活性を調整していかまたはPrP自身がSOD様の酵素活性を持っていることが示唆された。PrPは銅と結合することが報告されているが、銅代謝を制御することによりSODの活性を制御し、活性酸素の消去に関わっている可能性が考えられた。


• プリオン蛋白質関連神経細胞死発現機構に関する研究

  佐伯 圭一

  日本学術振興会, 科学研究費助成事業, 特定領域研究(C), 東京大学, 2001 - 2001

  プリオン病で見られる神経変性の発現機構には正常型のプリオン蛋白と病原体の本体と見られる異常型のプリオン蛋白の機能が密接に関係していると考えられている。本研究ではプリオン蛋白遺伝子欠損不死化神経細胞株におけるアポトーシス抑制能について検討した。特にアポトーシス抑制関連因子(Bcl-2,Bcl-XL, SOD及びTNF-alpha)との相互作用について検討した。また、プリオン蛋白遺伝子欠損マウス、野生型マウス、プリオン蛋白遺伝子欠損不死化神経細胞株およびプリオン蛋白遺伝子再構築不死化神経細胞株を用いて、EMC-B株、コクサッキーB1,B2,B3,B5,B6株感染時における宿主細胞内プリオン蛋白の機能を推定、検討を行なった。プリオン蛋白遺伝子欠損不死化神経細胞株に上記のBcl-2,Bcl-XL, SOD-1 cDNAを導入したところ、PrP cDNAを導入した細胞株で認められる無血清培地下でのアポトーシス抑制効果が認められた。プリオン蛋白遺伝子を再導入した細胞株におけるBcl-2,Bcl-XL及びSOD蛋白の産生を検討したところ、産生に変化は認められなかった。ところが、細胞内成分および細胞膜成分のSOD活性を測定したところ、SOD活性の上昇を認めた。正常プリオン蛋白は銅結合蛋白であることから、SOD分子に銅を供給する銅シャペロン分子であることが推測され、プリオン蛋白を発現によって細胞内SOD活性が上昇したと考えられた。また、細胞膜成分のSOD活性も上昇したことから、プリオン蛋白自体が膜上でSOD様の活性を持っていると予想された。


• ウイルス誘発性アポトーシスにおける正常プリオン蛋白の機能に関する研究

  佐伯 圭一

  日本学術振興会, 科学研究費助成事業, 奨励研究(A), 東京大学, 2000 - 2001

  プリオン蛋白欠損不死化神経細胞株を用いてコクサキーウイルスおよび脳心筋炎ウイルスB株(EMC-B)の感受性を検討するとともに、プリオン蛋白遺伝子再導入株を作製し、ウイルスの感受性変化について解析を行なった。プリオン蛋白欠損不死化神経細胞株と他のウイルス分離に一般的に使われている細胞株を比較したところ、プリオン蛋白欠損不死化神経細胞株は脳心筋炎ウイルス及びコクサッキーウイルスそれぞれの感染において感受性を有していた。また、ウイルス増殖が他の細胞株と比較し早く、最終的なウイルス力価も高かった。さらに細胞変性効果出現時期においても他の細胞株と比較して早く、ウイルス分離のための細胞株としてプリオン蛋白欠損不死化神経細胞株の有用性が示された。感染時におけるプリオン蛋白欠損不死化神経細胞の細胞変性効果誘導が、プリオン蛋白の再発現化に伴い影響を受けるのかを解析したところ、プリオン蛋白遺伝子を再導入した細胞は細胞変性効果の出現が遅れ、また、アポトーシス抵抗性を示した。このことより、ウイルス誘発性のアポトーシスにおいてプリオン蛋白が抑制的に働いていることが示唆された。ウイルス感染時における宿主細胞プリオン蛋白の機能を推定するために、EMC-Bウイルス感染後経時的にマウスを屠殺・剖検し、組織標本を作製した後、細胞組織学的、ウイルス病理学的に脳炎症反応の解析を行なった。アポトーシス神経細胞の出現時期は、野生型マウスよりもプリオン蛋白欠損マウスで早期に観察された。また、浸潤細胞のアポトーシスもプリオン蛋白欠損マウスで早期に観察された。これらのことからプリオン蛋白の機能として抗酸化作用が考えられ酸化ストレスによるアポトーシスと密接に関連していることが示唆された。


• Effect of Prnp gene to prion protein expression

  ONODERA Takashi, SASAKI Keiichi, MATSUMOTO Yoshitsugu

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), THE UNIVERSITY OF TOKYO, 2000 - 2001

  Some of the authors previously demonstrated the relation between cellular prion protein (PrP^c) and apoptosis using immortalized prion protein gene (Prnp)-deficient neuronal cells. However, the mechanism(s) by which PrP^c prevents apoptosis remains unclear. In this study, the authors analyzed apoptosis of Prnp^<-/-> cells using gene transfer of apoptosis-related genes. Transfection of Prnp^<-/-> cells with bcl-2, bcl-x_L or SOD-1, which encodes Cu/Zn superoxide dismutase (SOD), inhibited apoptosis induced by serum deprivation. As serum deprivation decreased Bcl-2 and Bcl-x_L proteins in-Prnp^<-/-> cells, these cells are thought to die via apoptosis pathways regulated by the Bcl-2 family and intracellular superoxides. Re-introduction of Prnp upregulated SOD activity and eliminated superoxide anion generation without inducing changes hi Bcl-2, Bcl-x_L and Cu/Zn SOD expression levels. As PrP^c was bound to copper in the octapeptide repeats, these results suggested that PrP^c inhibited apoptosis by upregulation of SOD activity due to copper metabolism regulation.


• Production of MHV-resistance mouse by embryo technology

  TAGUCHI Fumihiro, ITOH Toshio, YAMADA Yasuko, YAGAMI Kenichi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Institute of Neuroscience, NCNP, 1996 - 1998

  We have studied the resistance mechanisms of mice to mouse hepatitis virus (MHV) in order to produce MHV-resistance mouse strain by embryo technology. SJL and BALB/c mice are resistant and susceptible to MHV, respectively. We speculated that the difference in susceptibility resulted from MHV receptor expressed in these mice from the following findings. 1) MHV resistance is controlled by a single autosomal gene on chromosome 7 on which MHV-receptor gene is also mapped. 2) The fact that susceptibility is dominant over the resistance fits the concept that a dominant gene product is a host factor to make mice susceptible ; MHV receptor is one of the candidates. We found that the receptor MHVR1 expressed in BALB/c was 10 to 100 fold more efficient for MHV binding than the MHVR2 expressed in SJL.This difference could be responsible for the different susceptibility between these mice. We have also found that MHV-resistance gene and MHV-receptor gene were tightly linked, within 0.94 cM distance on chromosome 7, by using F2 and backcrossed progenies between BALB/c x SJL.This suggests the possibility that MHV-resistance gene is identical to MHV-receptor gene. To examine such possibility, experiments are under progress to replace MHVR1 and MHVR2 genes by gene replacement. The susceptibility of SJL whose MHVR2 is replaced by MHVR1 as well as BALB/c whose MHVR1 is replaced by MHVR2 will conclude whether our hypothesis is right or not. To make MHV-resistance mice, experiment is currently in progress to knockout the MHV-receptor genes by gene targeting.