研究活動情報

• 2025年07月 The 11th EAFES International Congresas, 最優秀賞, 環境DNA解析を用いた水田における両生類の密度と景観要因との関係

  Akinori Ogura, Qianqian WU, Ryohei NAKAO, Atushi USHIMARU, Toshifumi, MINAMOTO


• 2024年12月 日本環境DNA学会, 優秀賞: 日本環境DNA学会第7回環境DNA学会つくば大会, 琵琶湖におけるブラックバス類の時空間的分布動態の解明

  岡田淳史, 脇村圭, 内井喜美子, 鄔倩倩, 源利文


• 2022年11月 日本環境DNA学会「あなたが主役のワークショップ」, 優秀賞, 環境DNAメタバーコーディングによる深海無脊椎動物 の検出。

  鄔倩倩, 西澤崚平, 藤原義弘, 土田真二, 吉田尊雄, 河戸勝, 駒井智幸, 源利文

• Wild Chinese giant salamander persist in Zhangjiajie, Hunan, China: Evidence from 10 years of monitoring

  Zhiqiang Liang, Qianqian Wu, Li Zou, Zhiyong Deng, Jinxin Zhou, Xuan Xie, Linmei Han, Chongrui Wang, Xunhua Liu, Mingqiu Liu, Qiwei Wei

  2026年06月, Global Ecology and Conservation, パスワードが無い

  [査読有り]

  研究論文（学術雑誌）

  添付ファイルのURL


• Stemflow for detecting mammalian environmental DNA: a case study in a zoo

  Yuya Ishihara, Kazumi Aoki, Ayumi Sakata, Masayuki K. Sakata, Kyohei Hamano, Qianqian Wu, Masaki Miya, Toshifumi Minamoto

  2026年04月, Metabarcoding and Metagenomics, パスワードが無い

  [査読有り]

  研究論文（学術雑誌）

  添付ファイルのURL


• QuickConc Vacuum: A High-Sensitive Cationic-Assisted Method for Large-Volume eDNA Concentration and Sensitive Biodiversity Monitoring

  Tomohiro Kuroita, Qianqian Wu, Ryo Iwamoto, Toshifumi Minamoto

  2026年


• Environmental DNA Based Assessment of Fish Diversity in the Yarlung Zangbo River, Tibetan Plateau

  Haiyu Wang, Qianqian Wu, Shenhui Li, Hongyu Jin, Zepeng Zhang, Wanqiao Lu, Fei Liu, Guishuang Wang, Lei Li

  2025年11月, Ecology and Evolution

  [査読有り]

  研究論文（学術雑誌）


• QuickConc: A Rapid, Efficient, and Power‐Free eDNA Concentration Method With Cationic‐Assisted Capture

  Tomohiro Kuroita, Qianqian Wu, Ryo Iwamoto, Toshifumi Minamoto

  ABSTRACT Environmental DNA (eDNA) analysis is effective for non‐invasive biodiversity monitoring, as it reveals species distribution and abundance without ecosystem disruption. Four key steps in eDNA analysis include water preservation, DNA capture, DNA extraction, and detection. Among these, the capture of eDNA has attracted significant research interest due to the variability of water samples. Although various methods for eDNA concentration have been developed, including filtration using disc or cartridge filters and passive samplers, no single method is universally applicable because of the variabilities of eDNA distribution and water characteristics, including turbidity levels. Therefore, the development of alternative eDNA concentration methods is important for advancing eDNA research. This study introduces QuickConc, a novel nucleic acid capture method that combines an enhancer of benzalkonium chloride with dispersed silica glass fibers, allowing better binding between nucleic acids and glass fibers. Our results indicate that this approach enhances eDNA capture and extraction efficiency by likely improving the interaction between glass fibers and eDNA. We tested QuickConc in three environments using qPCR and metabarcoding. QuickConc yielded 1.3–3 times more total eDNA compared to glass fiber filtration and Sterivex methods under our experimental settings. Species‐specific qPCR results showed that QuickConc detected 2–10 times higher copy numbers compared to the other two methods. Metabarcoding analyses using the MiFish method revealed that the number of fish species detected in river water was higher with QuickConc compared to other methods, while in sea water, the number of fish species detected was similar to the glass fiber filtration and Sterivex methods. QuickConc offers new options for eDNA analysis for biodiversity monitoring and conservation strategies.

  Wiley, 2025年10月, Ecology and Evolution, 15(10) (10)

  [査読有り]

  研究論文（学術雑誌）

  神戸大学リポジトリ（Kernel）へのリンク


• Climate Change Impacts on Antarctic Fish Diversity: A Circumpolar Perspective From eDNA Metabarcoding Assessment

  Hai Li, Fang Yang, Yuzhuo Liao, Xiang Zhang, Yuan Li, Rui Wang, Ran Zhang, Dong Li, Qianqian Wu, Longshan Lin

  ABSTRACT Aim Recent climate‐driven changes in the Antarctic marine environment, marked by regional differences between West and East Antarctica, may alter ecosystem dynamics. As key components of the food web, fish might reflect these changes. This study examines fish biodiversity patterns across four typical Antarctic seas by integrating eDNA analysis with bottom trawl surveys, focusing on biodiversity shifts and range alterations in response to varying levels of climate change exposure. Location Four ecologically distinct Antarctic regions: The Adjacent Waters of Antarctic Peninsula (AAP), the Amundsen Sea‐Bellingshausen Sea (ASBS), the Prydz Bay‐Cooperation Sea (PBCS), and the Cosmonaut Sea (CS). Methods During the 37th Chinese National Antarctic Research Expedition, we integrated eDNA metabarcoding (from both pelagic and benthic water layers) with bottom trawl surveys across four regions (AAP, ASBS, PBCS, and CS) to characterise regional fish community. Results Environmental DNA metabarcoding and trawl surveys detected 40 and 27 Antarctic fish species, respectively. Environmental DNA proved effective in complementing traditional methods and capturing early life stages, especially with a refined local reference database. Species belonging to the suborder Notothenioidei dominated across all surveyed regions. Ten species exhibited range extensions beyond previously documented distributions, likely reflecting historical data gaps, with the exception of Patagonotothen sp. Main Conclusions eDNA metabarcoding showed potential for monitoring Antarctic ichthyoplankton. Fish diversity in the four studied seas aligns with the general pattern in the Southern Ocean, while ASBS was identified as a previously overlooked fish diversity hotspot. The southward detection of Patagonotothen sp. may signal climate‐driven shifts in species distribution. Future research integrating eDNA with interdisciplinary approaches holds promise for advancing the monitoring of climate change impacts on Antarctic marine ecosystems.

  Wiley, 2025年07月, Diversity and Distributions, 31(7) (7)

  [査読有り]

  研究論文（学術雑誌）

  神戸大学リポジトリ（Kernel）へのリンク


• Development of universal PCR primers for the environmental DNA metabarcoding of cephalopod (Mollueca) diversity

  Qianqian Wu, Tomoyuki Nakano, So Ishida, Tomoyuki Komai, Yoshihiro Fujiwara, Takao Yoshida, Masaru Kawato, Shin-ichiro Oka, Katsunori Fujikura, Masaki Miya, Toshifumi Minamoto

  Elsevier BV, 2025年03月, Marine Environmental Research, 208, 107094 - 107094, 英語

  研究論文（学術雑誌）

  神戸大学リポジトリ（Kernel）へのリンク


• Investigation on the Distribution of Bangana Tungting in Yuanshui Unique Fish Species National Aquatic Germplasm Resources Reserve Using Environmental DNA Technology

  Lu Tian, Qianqian Wu, Li Zou, Jinxin Zhou, Chongrui Wang, Linmei Han, Zaiquan Zhang, Xing Xiang, Mingqiu Liu, Zhifeng Feng, Zhonggui Xie, Zhiqiang Liang

  2024年12月, Ecology and Evolution

  [査読有り]

  研究論文（学術雑誌）

  神戸大学リポジトリ（Kernel）へのリンク


• Risk Screening of Invasive Aquatic Species and a Survey of Fish Diversity Using Environmental DNA Metabarcoding Analysis in Shanghai

  Ruohan Yu, Qianqian Wu, Fan Li, Aibin Zhan, Jinxin Zhou, Shan Li

  2024年01月, Diversity

  [査読有り]

  研究論文（学術雑誌）

  神戸大学リポジトリ（Kernel）へのリンク


• Opposite trends in environmental DNA distributions of two freshwater species under climate change

  Qianqian Wu, Jinxin Zhou, Tatsuya Komoto, Toshiyuki Ishikawa, Naoshige Goto, Masayuki K. Sakata, Daisuke Kitazawa, Toshifumi Minamoto

  2023年09月, Ecosphere

  [査読有り]

  研究論文（学術雑誌）


• Improvement of recovery yield of macro-organismal environmental DNA from seawater samples

  Qianqian Wu, Toshifumi Minamoto

  2023年05月, Analytical Sciences

  [査読有り]

  研究論文（学術雑誌）

  神戸大学リポジトリ（Kernel）へのリンク


• Estimating the spawning activity of fish species using nuclear and mitochondrial environmental DNA concentrations and their ratios

  Luhan Wu, Qianqian Wu, Takashi Inagawa, Jiro Okitsu, Shogo Sakamoto, Toshifumi Minamoto

  Abstract During spawning activity, fish release large amounts of sperm and eggs into the water, which has been assumed to cause an increase in environmental DNA (eDNA) levels and nuclear DNA/mitochondrial DNA ratios. To test whether these assumptions are valid and whether nuclear and mitochondrial eDNA analysis can be used to monitor the spawning activity of freshwater fish, we conducted field eDNA surveys and traditional surveys using common carp (Cyprinus carpio), largemouth bass (Micropterus salmoides) and bluegill sunfish (Lepomis macrochirus) as model species. Fish spawning periods were estimated based on age, as estimated using the body lengths of juveniles collected in the Miharu reservoir in Fukushima, Japan. The results showed that the main spawning periods of largemouth bass and bluegill sunfish were from April to July and from July to August, respectively. Field eDNA surveys were conducted in the Hebisawagawa front reservoir, which is connected to the Miharu reservoir. From March to August 2019 and 2020, weekly eDNA sampling was conducted at three sites, and daily sampling was conducted at six sites from 23 June to 3 July 2020. The eDNA concentrations of the nuclear internal transcribed spacer 1 (ITS1) and mitochondrial cytochrome B (CytB), as well as the ITS1/CytB ratio, were measured for each of the three fish in each water sample. Water temperature had a statistically significant effect on eDNA concentration, probably reflecting the relationship between water temperature and spawning. We created generalised additive mixed models to estimate spawning activity periods based on weekly eDNA data. The estimated periods of spawning activity for common carp, largemouth bass and bluegill sunfish were March to May, May to July, and May to August, respectively. The estimated spawning periods coincided with known fish ecology or the results of traditional methods. This method also has been applied to daily eDNA samples, showing the feasibility of high‐resolution estimation of spawning activity. For common carp and bluegill sunfish, we were able to estimate the spawning period using this method. Although the method is affected by biomass and the diffusion and degradation of eDNA, it has the potential to accurately estimating spawning activities. These then can be estimated without conducting laborious traditional surveys, facilitating the monitoring of reproduction by rare, invasive or important fishery species. Further research on the diffusion distance and degradation time of the eDNA concentration peak caused by fish spawning activity may improve the accuracy of monitoring.

  Wiley, 2022年11月, Freshwater Biology, 68(1) (1), 103 - 114

  [査読有り]


• Application of environmental DNA metabarcoding in a lake with extensive algal blooms

  Qianqian Wu, Masayuki K. Sakata, Deyi Wu, Hiroki Yamanaka, Toshifumi Minamoto

  2021年08月, Limnology

  [査読有り]

  研究論文（学術雑誌）


• Hepatopancreas Transcriptome Profiling Analysis Reveals Physiological Responses to Acute Hypoxia and Reoxygenation in Juvenile Qingtian Paddy Field Carp Cyprinus carpio var qingtianensis

  Ming Qi, Qianqian Wu, Tao Liu, Yiling Hou, Yixin Miao, Menghong Hu, Qigen Liu

  Frontiers Media {SA}, 2020年09月, Frontiers in Physiology, 11

  [査読有り]

  研究論文（学術雑誌）


• Habitat selection and migration of the common shrimp, Palaemon paucidens in Lake Biwa, Japan—An eDNA‐based study

  Qianqian Wu, Ken Kawano, Toshiyuki Ishikawa, Masayuki K. Sakata, Ryohei Nakao, Masayoshi K. Hiraiwa, Satsuki Tsuji, Hiroki Yamanaka, Toshifumi Minamoto

  Wiley, 2019年05月, Environmental DNA, 1(1) (1), 54 - 63

  [査読有り]

  研究論文（学術雑誌）


• The life history with seasonal migration of the lacustrine shrimp Palaemon paucidens in an ancient lake in Japan

  Qianqian Wu, Yasuoki Takami, Toshifumi Minamoto, Toshiyuki Ishikawa

  Wiley, 2019年04月, Ecosphere, 10(4) (4), 英語

  [査読有り]

  研究論文（学術雑誌）


• Environmental DNA reveals nonmigratory individuals of Palaemon paucidens overwintering in Lake Biwa shallow waters

  Qianqian Wu, Ken Kawano, Yoshitoshi Uehara, Noboru Okuda, Masamichi Hongo, Satsuki Tsuji, Hiroki Yamanaka, Toshifumi Minamoto

  University of Chicago Press, 2018年06月, Freshwater Science, 37(2) (2), 307 - 314, 英語

  [査読有り]

  研究論文（学術雑誌）

• 高効率eDNA濃縮手法QuickConcの大容量試料への適用

  黒板智博, 黒板智博, WU Qianqian, 岩本遼, 岩本遼, 源利文

  2025年, 応用生態工学会大会講演要旨集, 28th


• 大量の水試料中のDNA高効率・迅速濃縮手法の開発

  岩本遼, 黒板智博, WU Qianqian, 源利文

  2025年, 日本微生物生態学会大会(Web), 38th


• 高収率な環境DNA濃縮手法QuickConcの開発

  岩本遼, 岩本遼, 黒板智博, 黒板智博, う倩倩, 源利文

  2025年, 日本水産学会大会講演要旨集(CD-ROM), 2025


• 迅速,効率的なeDNA核酸濃縮手法(QuickConc)の開発

  岩本遼, 黒板智博, WU Qianqian, 源利文

  2024年, 日本分子生物学会年会プログラム・要旨集(Web), 47th

• 環境DNAを用いた陸上哺乳類と鳥類の検出方法の開発

  中村仁湖, 橋本渚, 坂田雅之, 邬倩倩, 源利文

  第6回環境DNA学会九州大会, 2023年12月, 日本語

  シンポジウム・ワークショップパネル（指名）


• 環境DNA分析と生態モデルによる気候変動下における生物の時空間分布予測

  邬倩倩, 周金鑫, 河本達也, 石川俊之, 後藤直成, 坂田雅之, 北澤大輔, 源利文

  第6回環境DNA学会九州大会, 2023年12月, 日本語

  ポスター発表

• 日本環境DNA学会

  2018年11月 - 現在


• 日本生態学会

  2016年03月 - 現在

• 環境DNA分析と生態モデルによる気候変動下における魚類の時空間分布予測

  鵜倩倩

  公益財団法人中辻創智社, 神戸大学人間発達環境学研究科, 2024年07月 - 2026年03月, 研究代表者


• 環境DNAメタバーコーディングとQGISを活用した水生コウチュウ目の多様性解析

  鄔 倩倩

  関西の自然保護・自然環境保全に関する調査研究, 神戸大学, 2024年04月 - 2025年03月, 研究代表者


• 湖底の光環境は生産性を通じて生物多様性に影響するか？

  石川 俊之, 邬 倩倩

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 滋賀大学, 2015年04月01日 - 2019年03月31日

  透明度の増加がみられ湖底の光量が増加していると考えられる琵琶湖において、光量と底泥上の藻類量、底生動物の現存量との関係を現地調査によって解析した。光量と底泥上の藻類量のそれぞれの季節変化の間には正の相関関係がみられ、光量の増加が藻類量を増加する可能性が示唆された。底生動物の現存量は分類群ごとに相関のある要因が異なり、カワニナ類において現存量と底泥上の藻類量との正の相関がみられた。 大型甲殻類（エビ類、ヨコエビ類）について、分布の変化やその要因、種間関係についての野外調査を行い、特にヨコエビ類の外来種による影響を示すことができた。


• 琵琶湖産スジエビにおける集団間の生態的・遺伝的分化の解明

  鄔 倩倩

  関西の自然保護・自然環境保全に関する調査研究, 神戸大学, 2017年04月 - 2018年03月, 研究代表者

• 北太平洋におけるアカイカ（ Ommastrephes bartrami）の検出系の開発、およびPCR増幅条件と応用に関する研究

  Xinjun, Chen, Q. Wu, Y. Liu, B. Liu, C. Wang

  特願LU102260, 2020年12月02日, 上海海洋大学, 特開LU102260, 2021年12月02日

  特許権