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川本 晋一郎医学部附属病院 輸血・細胞治療部講師
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■ 論文- Monovalent Omicron XBB.1.5 mRNA vaccines were newly developed and approved by the FDA in Autumn 2023 for preventing COVID-19. However, clinical efficacy for these vaccines is currently lacking. We previously established the quantification of antigen-specific antibody sequence (QASAS) method to assess the response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination at the mRNA level using B-cell receptor (BCR) repertoire assay and the coronavirus antibody database (CoV-AbDab). Here, we used this method to evaluate the immunogenicity of monovalent XBB.1.5 vaccines. We analyzed repeated blood samples of healthy volunteers before and after monovalent XBB.1.5 vaccination (BNT162b2 XBB.1.5 or mRNA-1273.815) for the BCR repertoire to assess BCR/antibody sequences that matched SARS-CoV-2-specific sequences in the database. The number of matched unique sequences and their total reads quickly increased 1 week after vaccination. Matched sequences included those bound to the Omicron strain and Omicron XBB sublineage. The antibody sequences that can bind to the Omicron strain and XBB sublineage revealed that the monovalent XBB.1.5 vaccines showed a stronger response than previous vaccines or SARS-CoV-2 infection before the emergence of XBB sublineage. The QASAS method was able to demonstrate the immunogenic effect of monovalent XBB.1.5 vaccines for the 2023-2024 COVID-19 vaccination campaign.2024年08月, EJHaem, 5(4) (4), 661 - 668, 英語, 国際誌研究論文(学術雑誌)
- BACKGROUND: We previously demonstrated that CD34 + cell transplantation in animals healed intractable fractures via osteogenesis and vasculogenesis; we also demonstrated the safety and efficacy of this cell therapy in an earlier phase I/II clinical trial conducted on seven patients with fracture nonunion. Herein, we present the results of a phase III clinical trial conducted to confirm the results of the previous phase studies using a larger cohort of patients. METHODS: CD34 + cells were mobilized via administration of granulocyte colony-stimulating factor, harvested using leukapheresis, and isolated using magnetic cell sorting. Autologous CD34 + cells were transplanted in 15 patients with tibia nonunion and 10 patients with femur nonunion, who were followed up for 52 weeks post transplantation. The main outcome was a reduction in time to heal the tibia in nonunion patients compared with that in historical control patients. We calculated the required number of patients as 15 based on the results of the phase I/II study. An independent data monitoring committee performed the radiographic assessments. Adverse events and medical device failures were recorded. RESULTS: All fractures healed during the study period. The time to radiological fracture healing was 2.8 times shorter in patients with CD34 + cell transplantation than in the historical control group (hazard ratio: 2.81 and 95% confidence interval 1.16-6.85); moreover, no safety concerns were observed. CONCLUSIONS: Our findings strongly suggest that autologous CD34 + cell transplantation is a novel treatment option for fracture nonunion. TRIAL REGISTRATION: UMIN-CTR, UMIN000022814. Registered on 22 June 2016.2023年10月, BMC medicine, 21(1) (1), 386 - 386, 英語, 国際誌研究論文(学術雑誌)
- The use of anti-SARS-CoV-2 antibody products like tixagevimab/cilgavimab represents an important strategy to protect immunocompromised patients with haematological malignancies from COVID-19. Although patients who receive these agents should still be vaccinated, the use of tixagevimab/cilgavimab can mask the production of anti-spike antibody after vaccination, making it hard to assess vaccine response. We have newly established a quantification method to assess the response to SARS-CoV-2 vaccination at the mRNA level using B-cell receptor (BCR) repertoire assay and the Coronavirus Antibody Database (CoV-AbDab). Repeated blood samples before and after vaccination were analysed for the BCR repertoire, and BCR sequences were searched in the database. We analysed the number and percentage frequency of matched sequences. We found that the number of matched sequences increased 2 weeks after the first vaccination and quickly decreased. Meanwhile, the number of matched sequences more rapidly increased after the second vaccination. These results show that the postvaccine immune response can be assessed at the mRNA level by analysing the fluctuation in matching sequences. Finally, BCR repertoire analysis with CoV-AbDab clearly demonstrated the response to mRNA SARS-CoV-2 vaccination even after tixagevimab/cilgavimab administration in haematological malignancy patients who underwent allogeneic haematopoietic stem cell transplantation.2023年06月, British journal of haematology, 202(3) (3), 504 - 516, 英語, 国際誌研究論文(学術雑誌)
- Abstract Sinusoidal obstruction syndrome (SOS) is a fatal complication of hematopoietic stem cell transplantation (HSCT). Early diagnosis for SOS can improve clinical outcomes significantly. Here, we performed a retrospective study to investigate the Cairo diagnostic criteria, in which SOS was defined as the development of two or more in seven events, including transfusion‐refractory thrombocytopenia. Among 154 cases of allogeneic HSCT, 10 cases of SOS using the European Society for Blood and Marrow Transplantation criteria (EBMT16) as the reference standard were identified. The original Cairo criteria could diagnose SOS 5 days earlier than any other established criteria, with some false‐positive results (sensitivity = 100.0%; specificity = 72.2%). When the cutoff was set to three events for the Cairo criteria, the diagnosis of SOS could be made 3 days earlier than that using the EBMT16 criteria, with comparable precision (specificity = 86.1%). The accuracy of the Cairo criteria improved further when the cutoff point was set to four (specificity = 93.8%). The fulfillment of the Cairo criteria was associated with high mortality. Based on our results, the Cairo criteria were also considered clinically useful, especially at three or four cutoff points. Further studies are required to validate and refine the criteria.Wiley, 2023年06月, eJHaem研究論文(学術雑誌)
- In acute myeloid leukemia (AML), the heterogeneity of genetic and epigenetic characteristics makes treatment difficult. The prognosis for AML is therefore poor, and there is an urgent need for new treatments for this condition. Gemtuzumab ozogamicin (GO), the first antibody-drug conjugate (ADC), targets the CD33 antigen expressed in over 90% of AML cases. GO therefore has the potential to counter the heterogeneity of AML patients. However, a major clinical problem is that drug resistance to GO diminishes its effect over time. Here, we report that the inhibition of glycogen synthase kinase 3 (GSK3) alone overcomes several forms of GO resistance at concentrations without antileukemic effects. The GSK3 inhibitors tested significantly enhanced the cytotoxic effect of GO in AML cell lines. We elucidated four mechanisms of enhancement: (1) increased expression of CD33, the target antigen of GO; (2) activation of a lysosomal function essential for hydrolysis of the GO linker; (3) reduced expression of MDR1 that eliminates calicheamicin, the payload of GO; and (4) reduced expression of the anti-apoptotic factor Bcl-2. A similar combination effect was observed against patient-derived primary AML cells. Combining GO with GSK3 inhibitors may be efficacious in treating heterogeneous AML.2023年02月, EJHaem, 4(1) (1), 153 - 164, 英語, 国際誌研究論文(学術雑誌)
- 2023年02月, Internal Medicine, 英語Efficacy and Safety of Synbiotics in Patients Undergoing Autologous Hematopoietic Stem Cell Transplantation: A Randomized, Double-blinded, Placebo-controlled Pilot Study[査読有り]研究論文(学術雑誌)
- We previously reported that a second dose of BNT162b2 was safe and effective for allogeneic hematopoietic stem cell transplantation (HSCT) patients. Here, we investigated the safety and efficacy of a third dose of COVID-19 mRNA vaccine in allogeneic HSCT patients. Antibody titers against the S1 spike protein were measured using the QuaResearch COVID-19 Human IgM IgG ELISA kit. The previous study included 25 allogeneic HSCT patients who received two doses of BNT162b2. Following the exclusion of three patients because of the development of COVID-19 (n = 2) and loss to follow-up (n = 1), the study evaluated 22 allogeneic HSCT patients who received a third dose of COVID-19 mRNA vaccine (BNT162b2 [n = 15] and mRNA-1273 [n = 7]). Median age at the time of the first vaccination was 56 (range, 23-71) years. Five patients were receiving immunosuppressants at the third vaccination, namely calcineurin inhibitors (CI) alone (n = 1), steroids alone (n = 2), or CI combined with steroids (n = 2). Twenty-one patients (95%) seroconverted after the third dose. None of our patients had serious adverse events, new-onset graft-versus-host disease (GVHD), or GVHD exacerbation after vaccination. A third dose of the BNT162b2 and mRNA-1273 COVID-19 vaccines was safe and effective for allogeneic HSCT patients.2022年10月, Vaccines, 10(11) (11), 英語, 国際誌研究論文(学術雑誌)
- Springer Science and Business Media LLC, 2022年06月, Ann Hematol ., 101(6) (6), 1343 - 1344[査読有り]研究論文(学術雑誌)
- Anti-CD20 antibodies react with CD20 expressed not only on malignant B cells, but also on normal B cells. It has been reported that patients treated with anti-CD20 antibodies had an insufficient response to two-dose mRNA SARS-CoV-2 vaccination. To investigate the efficacy of a third dose in these patients, we investigated serum IgG antibody titers for the S1 protein after a third vaccination in 22 patients treated with the anti-CD20 antibody who failed two-dose vaccination. Results showed that overall, 50% of patients seroconverted. Although no patient who received the third dose within 1 year of the last anti-CD20 antibody administration showed an increase in S1 antibody titer, 69% of patients who received the third dose more than 1 year after the last anti-CD20 antibody administration seroconverted. Our data show that a third dose of vaccination is effective in improving the seroconversion rate in patients treated with the anti-CD20 antibody who failed standard two-dose vaccination.2022年06月, Vaccines (Basel), 10(6) (6), 965, 英語, 国際誌[査読有り]研究論文(学術雑誌)
- 2022年06月, Internal MedicineBiclonal diffuse large B-cell lymphoma commonly characterized by partial trisomy 18q involving MALT1 and BCL2[査読有り]
- 2022年02月, 日本検査血液学会雑誌, 23(1) (1), 19 - 27, 日本語当院における骨髄増殖性腫瘍のJAK2 V617F変異アレルバーデンの解析[査読有り]研究論文(学術雑誌)
- Patients who have undergone hematopoietic stem cell transplantation (HSCT) for hematological disease experience high mortality when infected by coronavirus disease 2019 (COVID-19). However, the safety and efficacy of the COVID-19 vaccine in HSCT patients remain to be investigated. We prospectively evaluated the safety and immunogenicity of the BNT162b2 mRNA COVID-19 vaccine (Pfizer BioNTech) in 25 Japanese allogeneic HSCT patients in comparison with 19 healthy volunteers. While anti-S1 antibody titers in almost all healthy volunteers after the second dose were higher than the cut-off value reported previously, levels in HSCT patients after the second dose were diverse. Nineteen patients (76%) had seroconversion of anti-S1 IgG. The median optical density of antibody levels in HSCT patients with low IgG levels (<600 mg/dL), steroid treatment, or low lymphocytes (<1000/μL) was significantly lower than that in the other HSCT patients. There were no serious adverse events (>Grade 3) and no new development or exacerbation of graft-versus-host disease after vaccination. We concluded that the BNT162b2 mRNA vaccine is safe and effective in Japanese allogeneic HSCT patients.2022年01月, Vaccines (Basel), 10(2) (2), 158, 英語, 国際誌[査読有り]研究論文(学術雑誌)
- We investigated the efficacy of BNT162b2 mRNA COVID-19 vaccine in patients with B-cell malignancies treated with anti-CD20 antibody. Although T-cell-mediated immune responses were detected even in patients receiving R-CHOP treatment, the S1 antibody titer following BNT162b2 vaccination remained only marginally increased for more than 3 years after the final dose of anti-CD20 antibody. We found no relationship between the percent of B-cells and S1 antibody titer. The duration of this suppression was much longer than we anticipated. Further protection and treatment strategies against COVID-19 for these patients are warranted.2022年01月, International journal of Hematology, 115(1) (1), 7 - 10, 英語, 国内誌[査読有り]研究論文(学術雑誌)
- 2022年01月, 血液内科, 84(1) (1), 6 - 11, 日本語AIHA診療における最近の動向[招待有り]論文集(書籍)内論文
- 2021年11月, Pharma Medica, 39(11) (11), 15 - 17, 日本語溶血性貧血[招待有り]論文集(書籍)内論文
- 2021年10月, Kobe Journal of Medical Science, 67(2) (2), E55 - E60Serum Cytokine Profiles of Rapid Recovery Patients with COVID-19: Series of 6 Cases[査読有り]
- (一社)日本血液学会, 2021年09月, 日本血液学会学術集会, 83回, PS - 6, 英語慢性GVHDに対する肝移植後の一過性末梢血マクロキメリズム(Transient macrochimerism following a liver transplant for hepatic GVHD after an allo-PBSCT)
- (一社)日本血液学会, 2021年09月, 日本血液学会学術集会, 83回, OS2 - 4, 英語自家造血幹細胞移植患者におけるsynbiotics投与の有効性についての臨床試験(Efficacy of synbiotics in auto-PBSCT patients: a prospective, double-blind, placebo-controlled trial)
- (一社)日本血液学会, 2021年09月, 日本血液学会学術集会, 83回, OS3 - 5, 英語同種移植におけるflash sensor-based glucose monitoringの安全性の検討(Safety and accuracy of flash sensor-based glucose monitoring devise in patients after Allo-HSCT)
- 2020年10月, 生体の科学, 71(5) (5), 458 - 459, 日本語自己免疫性溶血性貧血[招待有り]
- 2020年09月, 臨床血液, 61(9) (9), 1089 - 1097, 日本語自己免疫性溶血性貧血の診断と治療—続発性について考える—[査読有り][招待有り]研究論文(学術雑誌)
- 2020年05月, 臨床血液, 61(5) (5), 491 - 501, 日本語続発性自己免疫性溶血性貧血における自己抗体の標的分子の同定[査読有り][招待有り]研究論文(学術雑誌)
- Autoimmune hemolytic anemia (AIHA) is a rare comorbidity in colorectal cancer (CRC) and has an unknown etiology. Previously, we described an AIHA case secondary to CRC with ectopic band 3 expression. Herein, we investigated ectopic band 3 expression and erythrocyte membrane-bound IgG in a CRC cohort. Between September 2016 and August 2018, 50 patients with CRC and 26 healthy controls were enrolled in the present study. The expression of band 3 and SLC4A1 mRNA was observed in 97% of CRC surgical specimens. Although clinical AIHA was not observed in any patient with CRC, a direct antiglobulin test was positive in 10 of the patients in the CRC group (p = 0.01). Flow cytometry revealed significantly increased erythrocyte membrane-bound IgG among patients with CRC compared to healthy controls (mean ± standard deviation; 38.8 ± 4.7 vs. 29.9 ± 15.6, p = 0.012). Normocytic anemia was observed, including in cases negative for fecal occult blood, suggesting a shortened erythrocyte life-span due to increased membrane-bound IgG. Immunoprecipitation revealed increased anti-band 3 autoantibodies in patients' sera. Mouse experiments recapitulated this phenomenon. We also confirmed that band 3 expression is controlled by 5'AMP-activated protein kinase under hypoxic conditions. These findings increase our understanding of the etiology of cancer-related anemia.2020年05月, International journal of hematology, 111(5) (5), 657 - 666, 英語, 国内誌[査読有り]研究論文(学術雑誌)
- Springer Science and Business Media LLC, 2019年07月, International Journal of Hematology, 110(1) (1), 107 - 114研究論文(学術雑誌)
- A 45-year-old woman was diagnosed with hepatosplenic T-cell lymphoma (HSTCL), a rare subtype of peripheral T-cell lymphoma. She received different types of chemotherapy, but disease progression was observed. To reduce the tumor burden before an unrelated bone marrow transplantation, combination chemotherapy consisting of the gemcitabine, carboplatin, and dexamethasone (GCD) was administered as bridging therapy, resulting in a reduction in the number of lymphoma cells. We were then able to perform bone marrow transplantation. Although she experienced some adverse events, she successfully achieved long-term remission. We herein report a successful case of HSTCL treated with unrelated stem cell transplantation following the GCD regimen as bridging chemotherapy.2019年03月, Intern Med., 58(5) (5), 707 - 712, 英語, 国内誌[査読有り]研究論文(学術雑誌)
- BACKGROUND: Human herpesvirus 6 (HHV-6) encephalitis is a known life-threatening complication following allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, few studies have focused on the occurrence of HHV-6 encephalitis in patients receiving mycophenolate mofetil (MMF) combined with a calcineurin inhibitor as prophylaxis for graft-versus-host disease (GVHD). This study aimed to investigate the impact of MMF administered for GVHD prophylaxis in the occurrence of HHV-6 encephalitis after allo-HSCT and the characteristics of this condition. METHODS AND RESULTS: We retrospectively analyzed 73 patients who underwent allo-HSCT (83 transplants) at our hospital between April 2010 and December 2015. MMF (2-3 g/d) was administered along with a calcineurin inhibitor. Seven patients (8.0%) developed encephalitis due to HHV-6. The median period from allo-HSCT to the onset of HHV-6 encephalitis was 23 days (range, 17-98 days). The cumulative incidence of HHV-6 encephalitis on day 100 after treatment was 12% and 6% in patients who underwent cord blood transplantation (CBT) and non-CBT (ie, bone marrow transplantation and peripheral blood stem cell transplantation), respectively (P = 0.344). Neurological symptoms of encephalitis were more severe in non-CBT cases than those in CBT cases. All patients diagnosed with HHV-6 encephalitis were treated with ganciclovir or foscarnet. None of the enrolled patients died from HHV-6 encephalitis. CONCLUSIONS: Mycophenolate mofetil may have the potential to increase the frequency of severe HHV-6 encephalitis in patients undergoing CBT and non-CBT. Thus, MMF should be administered with caution, and patients should be monitored closely for HHV-6 encephalitis even those who did not undergo CBT.2019年02月, Transpl Infect Dis., 21(1) (1), e13024, 英語, 国際誌[査読有り]研究論文(学術雑誌)
- Cancer patients occasionally have anemia with high mean corpuscular volume in addition to iron deficiency anemia. Secondary autoimmune hemolytic anemia (AIHA) following cancer is also observed with low frequency. To date, no causal mechanisms for these disease states have been reported. Here, we present the case of an 80-year-old woman with AIHA that was resistant to prednisolone. Further examinations revealed primary adenocarcinoma of the sigmoid colon and primary squamous cell carcinoma in the right lung. After resections of these tumors, her anemia partially improved until a colon cancer-derived metastatic tumor was detected in the left lung. Immunoprecipitation of erythrocyte membrane proteins with an autoantibody followed by mass spectrometry/Western blotting identified band 3 as the target of the autoantibody. Immunohistochemical analysis revealed ectopic expression of band 3 in the colon adenocarcinoma. To our knowledge, this is the first report that identifies the cause in a case of anemia without bleeding in a cancer patient and that defines a mechanism underlying secondary AIHA following cancer progression.2019年01月, Hum Pathol, 83, 193 - 198, 英語, 国際誌[査読有り]研究論文(学術雑誌)
- Scopulariopsis alboflavescens is a soil saprophyte that is widely distributed in nature. Recently, there have been increasing number of reports of invasive infections with Scopulariopsis species in immunocompromised patients. In this report, we described an adult woman with acute myeloid leukemia and who developed S. alboflavescens pneumonia. Liposomal amphotericin B and voriconazole combination therapy was unsuccessful and the patient died because of pneumonia. Scopulariopsis is highly resistant to available antifungal agents and almost invariably fatal. This case report should alert clinicians to the importance of listing Scopulariopsis as a pathogenic fungus in immunocompromised patients.2018年12月, Int J Hematol., 108(6) (6), 658 - 664, 英語, 国内誌[査読有り]研究論文(学術雑誌)
- 2018年11月, Clin Case Rep, 7(1) (1), 155 - 159, 英語Toll-like receptor 4 promoter polymorphisms in a familial mediterranean fever patient with asymptomatic bacteriuria[査読有り]研究論文(学術雑誌)
- (一社)日本血液学会-東京事務局, 2018年09月, 臨床血液, 59(9) (9), 1756 - 1756, 英語TLR9の一塩基多型により無症候性CMV感染症を呈した再生不良性貧血の一例(Aplastic anemia that developed asymptomatic CMV infection due to SNPs of TLR9)
- (一社)日本血液学会-東京事務局, 2018年09月, 臨床血液, 59(9) (9), 1640 - 1640, 英語新たなZMYND11/MBTD1融合遺伝子の発現とt(10;17)(p15;q21)転座を認めたCD7+CD56+急性骨髄性白血病(Expression of a novel ZMYND11/MBTD1 fusion transcript in CD7+CD56+ AML with t(10;17)(p15;q21))[査読有り]
- Springer Science and Business Media LLC, 2018年07月, International Journal of Hematology, 108(1) (1), 58 - 65研究論文(学術雑誌)
- Springer Science and Business Media LLC, 2018年05月, Cancer Chemotherapy and Pharmacology, 81(5) (5), 839 - 846研究論文(学術雑誌)
- Lymph node infarction is very rare, and is frequently associated with neoplasms, such as malignant lymphoma and non-neoplastic disease, or interventions such as fine-needle aspiration (FNA). A 76-year-old-man presented with cervical lymph node swelling. Although FNA was performed, the findings were insufficient for a definitive diagnosis. Consequently, surgical biopsy of the cervical lymph node was performed, which revealed total infarction; a diagnosis of classical Hodgkin lymphoma was made later. Both lymphoma itself and FNA may cause total lymph node infarction, which makes diagnosis confusing. Therefore, it is important to repeat the biopsy rather than repeat FNA to correctly diagnose malignant lymphoma, including Hodgkin lymphoma.2018年03月, Journal of clinical and experimental hematopathology : JCEH, 58(1) (1), 24 - 26, 英語, 国内誌[査読有り]研究論文(学術雑誌)
- 2018年02月, J Clin Virol, 102, 50 - 55, 英語[査読有り]研究論文(学術雑誌)
- 2018年, 臨 床 病 理, 66, 962 - 967, 日本語加古川中央市民病院で経験した cup-like 核形態を有する急性骨髄性白血病 4 症例の検討[査読有り]研究論文(学術雑誌)
- Springer Science and Business Media LLC, 2017年12月, International Journal of Hematology, 106(6) (6), 729 - 731研究論文(学術雑誌)
- (一社)日本血液学会-東京事務局, 2017年11月, 臨床血液, 58(11) (11), 2289 - 2289, 日本語慢性C型肝炎治療後に発症したB細胞性前リンパ球性白血病の1例
- (一社)日本血液学会-東京事務局, 2017年09月, 臨床血液, 58(9) (9), 1763 - 1763, 日本語悪性リンパ腫患者におけるコリンエステラーゼ値層別化による腫瘍崩壊症候群発症リスクの後方視的解析
- 2017年07月, PLOS ONE, 12(7) (7), e0182021, 英語[査読有り]研究論文(学術雑誌)
- MDPI AG, 2017年02月, International Journal of Molecular Sciences, 18(3) (3), 486 - 486研究論文(学術雑誌)
- Constitutional translocations that coincide with t(9;22)(q34;q11.2) may lead to unnecessary treatments in chronic myeloid leukemia (CML) patients, as, under the standard criteria, a diagnosis of CML with additional chromosomal abnormalities indicates an accelerated phase (AP). In the present report, a 47-year-old male had pain in the right foot due to gout. Peripheral blood examination showed leukocytosis with left shift. Bone marrow aspiration revealed myeloid hyperplasia with megakaryocytosis. RT-PCR revealed the major BCR-ABL fusion transcript, and CML in the chronic phase was diagnosed, followed by nilotinib treatment. Although WBC counts decreased immediately, G-banding analysis showed 46,XY,t(8;22)(q24;q11.2),t(9;22)(q34;q11.2) [20]. The t(8;22)(q24;q11.2) translocation is known to be recurrent in Burkitt's lymphoma. The diagnosis was changed to CML in AP, leading to B-lymphoid crisis. Unexpectedly, the karyotype was 46,XY,t(8;22)(q24;q11.2) [20] in hematological complete remission, even after 3 months. Fluorescence in situ hybridization on metaphase spreads revealed the MYC signal on the der(22)t(8;22), indicating that the 8q24 breakpoint was centromeric to MYC at 8q24.21. G-banding analysis of phytohemagglutinin-stimulated peripheral blood T-lymphocytes also indicated 46,XY,t(8;22)(q24.1;q11.2). We conclude that the t(8;22) is constitutional in this patient. As the tumor suppressor gene TRC8/RNF139 is disrupted by constitutional t(8;22)(q24.13;q11.21) in dysgerminoma, it may be associated with the onset of CML.2017年02月, International Journal of Hematology, 105(2) (2), 226 - 229, 英語, 国内誌[査読有り]研究論文(学術雑誌)
- 一般社団法人 日本血液学会, 2017年, 臨床血液, 58(8) (8), 938 - 941, 日本語
A 49-year-old female was initially diagnosed with acute myeloid leukemia (AML) M4 with a CD45+CD13+CD33+CD34−HLA-DR+ immunophenotype. She underwent allogeneic bone marrow transplantation, but the disease recurred. The bone marrow was infiltrated with 87.0% blasts negative for myeloperoxidase (MPO) staining. Immunophenotyping by flow cytometry identified the presence of a CD45-negative blast population. These blasts exhibited a CD13+CD33+CD19−CD10−CD34−HLA-DR− immunophenotype. The lack of CD45 expression is often observed in B-cell acute lymphoblastic leukemia, whereas CD45-negative AML is extremely rare; only one older male with AML-M0 has been reported. In the present case, the CD45-negative blasts had an MPO−CD13+CD33+ phenotype, which is similar to AML-M0.
- Oncogene amplification is uncommon in acute myeloid leukemia (AML). Cytogenetically, it is primarily found as double minute chromosomes (dmin) or homogeneously staining regions (hsr). A 62-year-old woman was admitted to our hospital because of anemia and thrombocytopenia. Her bone marrow was hypercellular with 78.6% myeloperoxidase- positive blasts. Some had micronuclei. The patient was diagnosed with AML M2 and remains in complete remission (CR) after induction therapy. G-banding at diagnosis showed 51,XX,t(11;16)(q13;p11.2),+r1,+mar1×4. Spectral karyotyping confirmed t(11;16) and revealed that the ring and the marker chromosomes were derived from multiple copies of ring chromosome 8. Fluorescence in situ hybridization (FISH) with a MYC probe at 8q24 detected amplified MYC signals on 1 large and 4 small ring chromosomes 8. One MYC signal was deleted from one of the 2 chromosomes 8. FISH with a FUS probe at 16p11.2 showed monoallelic deletion of FUS. Immunohistochemistry demonstrated MYC protein overexpression at diagnosis and almost negative expression in CR. These results indicate that MYC amplification could occur in ring chromosomes without dmin. A cryptic MYC deletion suggests that an episome model could be applicable to MYC amplification in ring chromosomes as observed for dmin and hsr. Furthermore, considering 2 further reported cases, t(11;16)(q13;p11) may be a very rare but recurrent translocation in AML.2017年, Cytogenetic and genome research, 153(3) (3), 131 - 137, 英語, 国際誌研究論文(学術雑誌)
- A prospective study of the antiemetic effect of palonosetron in malignant lymphoma patients treated with the CHOP regimen.To identify strategies for reducing emesis induced by the CHOP regimen, which includes high-dose steroids, we prospectively evaluated the efficacy of palonosetron in Japanese patients. Palonosetron was administered at a dose of 0.75 mg via intravenous injection over 30 min before chemotherapy on day 1. Patients kept diaries of chemotherapy-induced nausea and vomiting (CINV) incidence from the start of chemotherapy until 168 h afterwards, in which they documented the occurrence and severity of nausea, vomiting, anorexia, and the use of rescue medication. The primary endpoint was the overall occurrence rate of nausea, vomiting, and anorexia; these rates were 56, 12, and 62 %, respectively, including all grades. The rates and severity of symptoms tended to worsen 120-168 h after completing oral prednisolone. We defined complete response (CR) as no vomiting and no use of rescue therapy. The CR rates of post palonosetron 0.75 mg treatment in the acute (0-24 h), delayed (24-168 h), and overall phases (0-168 h) were 86, 66, and 62 %, respectively. Antiemetic strategies of CHOP regimen for day 6 and, thereafter, should be investigated.2016年12月, International journal of hematology, 104(6) (6), 682 - 691, 英語, 国内誌研究論文(学術雑誌)
- 2016年11月, International journal of hematology, 104(5) (5), 531 - 533, 英語, 国内誌Sea-blue histiocytes in acute myeloid leukemia with trisomy 9.[査読有り]
- 2016年, Cytogenetic and Genome Research, 150(3-4) (3-4), 287 - 292研究論文(学術雑誌)
- 2016年, CYTOGENETIC AND GENOME RESEARCH, 149(3) (3), 165 - 170, 英語[査読有り]研究論文(学術雑誌)
- 2016年, CYTOGENETIC AND GENOME RESEARCH, 150(3-4) (3-4), 287 - 292, 英語[査読有り]研究論文(学術雑誌)
- 日本臨床検査医学会 ; 1953-, 2016年01月, 臨床病理, 64(1) (1), 21 - 26, 日本語骨髄異形成症候群(MDS)における好中球の異形成と、多項目自動血球分析装置XE-2100の指標(NEUT-X, NEUT-Y)との関連[査読有り]研究論文(学術雑誌)
- Oxford University Press, 2016年, Japanese Journal of Clinical Oncology, 46(5) (5), 448 - 452, 英語[査読有り]研究論文(学術雑誌)
- Springer-Verlag Tokyo, 2015年05月, International Journal of Hematology, 101(5) (5), 423 - 425, 英語研究論文(その他学術会議資料等)
- 2015年03月, 日本薬学会年会要旨集, (135年会4号) (135年会4号), 140, 日本語造血幹細胞移植患者に対する栄養管理への介入とその評価[査読有り]研究論文(学術雑誌)
- 2015年02月, JOURNAL OF DENTAL RESEARCH, 94(2) (2), 289 - 296, 英語[査読有り]研究論文(学術雑誌)
- 2015年, ACTA HAEMATOLOGICA, 134(2) (2), 76 - 79, 英語[査読有り]研究論文(学術雑誌)
- 2015年, J Clin Exp Hematop, 55(3) (3), 181 - 5, 英語Coexistent t(8;21)(q22;q22) Translocation and 5q Deletion in Acute Myeloid Leukemia[査読有り]研究論文(学術雑誌)
- 2015年01月, ANNALS OF HEMATOLOGY, 94(1) (1), 177 - 179, 英語[査読有り]研究論文(学術雑誌)
- (一社)日本血液学会-東京事務局, 2014年09月, 臨床血液, 55(9号) (9号), 1271 - 1271, 日本語肺、大腸重複癌に合併した自己免疫性溶血性貧血における自己抗体の標的分子の同定研究論文(その他学術会議資料等)
- 2014年, J Clin Exp Hematop, 54(2) (2), 167 - 70, 英語, 国内誌A new complex translocation t(8;11;21)(q22;q24;q22) in acute myeloid leukemia with RUNX1/RUNX1T1[査読有り]研究論文(学術雑誌)
- 2013年, Prensa Med, 99(10) (10), 英語Dasatinib Monotherapy to Maintain Complete Remission before Allogeneic Bone Marrow Transplantation under Monitoring of the Blastic Chronic Myelogenous Leukemia Biphenotypic Cells and Bcr-Abl Transcripts[査読有り]研究論文(学術雑誌)
- 2013年, INTERNAL MEDICINE, 52(10) (10), 1107 - 1110, 英語[査読有り]研究論文(学術雑誌)
- 2019年05月, Leukemia & lymphoma, 60(5) (5), 1294 - 1298, 英語, 国際誌[査読有り]速報,短報,研究ノート等(学術雑誌)
- 2018年11月, Leukemia & lymphoma, 59(11) (11), 2706 - 2710, 英語, 国際誌[査読有り]速報,短報,研究ノート等(学術雑誌)
- 2017年, 日本造血細胞移植学会総会プログラム・抄録集, 40th自家末梢血幹細胞移植における栄養状態と予後に関する後方視的解析
- (一社)日本血液学会-東京事務局, 2015年11月, 臨床血液, 56(11号) (11号), 2377 - 2377, 日本語30年来頸部腫瘤を有する患者より発症したDouble-hit lymphoma(DHL)の1例[査読有り]会議報告等
- 2015年02月, 日本内科学会雑誌, 104(Suppl.) (Suppl.), 241, 日本語化学療法中の固形腫瘍患者に対するインフルエンザワクチン2回接種法の有効性と安全性[査読有り]会議報告等
- 2015年, INTERNAL MEDICINE, 54(16) (16), 2057 - 2060, 英語[査読有り]速報,短報,研究ノート等(学術雑誌)
- 2014年12月, BLOOD, 124(21) (21), 英語Efficacy of the Two-Dose Influenza Vaccine in Cancer Patients Receiving Chemotherapy: A Prospective Study研究発表ペーパー・要旨(国際会議)
- (一社)日本血液学会-東京事務局, 2014年11月, 臨床血液, 55(11号) (11号), 2339 - 2339, 日本語腹部DLBCL病変出現1ヵ月前に発症したNeurolymphomatosisの1例会議報告等
- (一社)日本血液学会-東京事務局, 2013年09月, 臨床血液, 54(9) (9), 1203 - 1203, 日本語チロシンキナーゼ型受容体C-KIT変異による標的遺伝子制御機構の解析
- (一社)日本検査血液学会, 2012年06月, 日本検査血液学会雑誌, 13(学術集会) (学術集会), S144 - S144, 日本語白血病幹細胞樹立の試み
- 日本無菌生物ノートバイオロジー学会, 2005年12月01日, 無菌生物 = Japanese journal of germfree life and gnotobiology, 35(2) (2), 122 - 125, 日本語血液疾患に対するミニ移植
- 日本臨床血液学会, 1999年01月30日, 臨床血液, 40(1) (1), 9 - 15, 日本語同種骨髄移植におけるニューキノロンを用いた腸内無菌化法 -トブラマイシン, バンコマイシンを用いた腸内無菌化法との感染頻度の比較-
- APBMT, 2018年11月, 英語, アジア太平洋骨髄移植学会, Taipei, 国際会議Nutrition and Rehabilitation of Hematopoietic Stem Cell Transplantation Patientsポスター発表
- 第80回日本血液学会学術集会, 2018年10月, 日本語, 日本血液学会, 大阪, 国内会議新たなZMYND11/MBTD1融合遺伝子の発現とt(10;17)(p15;q21)転座を認めたCD7+CD56+急性骨髄性白血病ポスター発表
- 第80回日本血液学会学術集会, 2018年10月, 日本語, 日本血液学会, 大阪, 国内会議TLR9の一塩基多型により無症候性CMV感染症を呈した再生不良性貧血の一例.ポスター発表
- 第80回日本血液学会学術集会, 2018年10月, 英語, 日本血液学会, 大阪, 国内会議Aplastic anemia that developed asymptomatic CMV infection due to a single nucleotide polymorphism of TLR9ポスター発表
- 第16回日本臨床腫瘍学会, 2018年07月, 日本語, 日本臨床腫瘍学会, 神戸, 国内会議Flow cytometric measurement of erythrocyte membrane-bound IgG: A potential diagnostic method for colorectal cancerポスター発表
- Aemrican Association for Cancer Research Annual Meeting 2018, 2018年04月, 英語, American Association for Cancer Research, シカゴ, 国際会議Flow cytometric measurement of erythrocyte membrane-bound IgG: A potential diagnostic method for colorectal cancerポスター発表
- 第40回日本造血細胞移植学会総会, 2018年02月, 日本語, 日本造血細胞移植学会, 札幌, 国内会議非血縁者間骨髄移植前の病勢コントロールとしてGCD療法が有効であった肝脾原発T細胞性リンパ腫の一例ポスター発表
- 第40回日本造血細胞移植学会総会, 2018年02月, 日本語, 日本造血細胞移植学会, 札幌, 国内会議同種造血幹細胞移植の経過中に集中治療を要した患者の検討口頭発表(一般)
- 第40回日本造血細胞移植学会総会, 2018年02月, 日本語, 日本造血細胞移植学会, 札幌, 国内会議造血幹細胞移植病棟における疑義照会の後方視的検討口頭発表(一般)
- 第40回日本造血細胞移植学会総会, 2018年02月, 日本語, 日本造血細胞移植学会, 札幌, 国内会議自家末梢血幹細胞移植における栄養状態と予後に関する方視的解析ポスター発表
- 第40回日本造血細胞移植学会総会, 2018年02月, 日本語, 日本造血細胞移植学会, 札幌, 国内会議血縁ドナーにおける血液成分分離装置Spectra OptiaとCOBE Spectraの採取データの比較検討ポスター発表
- ASBMT/CIBMTR tandem meetings 2018, 2018年02月, 英語, ASBMT/CIBMTR, Salt Lake City, USA, 国際会議Pharmacokinetics of intravenous mycophenolate mofetil after hematopoietic stem cell transplantation in Japanese populationポスター発表
- 第40回日本造血細胞移植学会総会, 2018年02月, 日本語, 日本造血細胞移植学会, 札幌, 国内会議Pharmacokinetics of intravenous mycophenolate mofetil after cord blood transplantation in Japanese口頭発表(一般)
- 第218回近畿地方会, 2017年12月, 日本語, 日本内科学会近畿支部, 神戸, 国内会議悪性リンパ腫との鑑別に難渋した多中心性細網組織球症(MRH)の1例口頭発表(一般)
- 第79回日本血液学会学術集会, 2017年10月, 日本語, 日本血液学会, 東京, 国内会議Retrospective analysis of TLS risk in lymphoma patients stratified by cholinesterase levelポスター発表
- 第79回日本血液学会学術集会, 2017年10月, 英語, 日本血液学会, 東京, 国内会議Retrospective analysis of the ESHAP regimen for malignant lymphoma in Kobe University Hospital.ポスター発表
- 第79回日本血液学会学術集会, 2017年10月, 日本語, 日本血液学会, 東京, 国内会議MYC amplification in the form of ring chromosomes 8 in acute myeloid leukemia with t(11;16)(q13;p11)ポスター発表
- 第76回日本癌学会学術集会, 2017年09月, 日本語, 日本癌学会, 神奈川(横浜), 国内会議c-Kit遺伝子変異細胞株においてmicroRNA-7はRB1発現を抑制し染色体不安定性をもたらすポスター発表
- 第15回日本臨床腫瘍学会学術集会, 2017年07月, 日本語, 日本臨床腫瘍学会, 神戸, 国内会議Establishment and Gene Expression Analysis of a Double-Hit Lymphoma Cell Lineポスター発表
- 第107回近畿血液学地方会, 2017年06月, 日本語, 日本血液学会, 京都, 国内会議慢性C型肝炎治療後に発症したB細胞性前リンパ球性白血病の一例口頭発表(一般)
- Annual Meeting of the American Association for Cancer Research 2017, 2017年04月, 英語, American Asociation for Cancer Research, Washington DC, USA, 国際会議MicroRNA-7 suppresses RB1 expression leading to chromosomal instability in leukemia cells harboring c-Kit mutationポスター発表
- 第39回日本造血細胞移植学会, 2017年03月, 日本語, 日本造血細胞移植学会, 島根, 国内会議難治性の口唇慢性GVHDに対して湿潤療法が著効した一例ポスター発表
- 第39回日本造血細胞移植学会総会, 2017年03月, 日本語, 日本造血細胞移植学会, 松江, 国内会議シクロスポリンAが自家末梢血幹細胞移植後の再発に有効であった皮膚γδT細胞リンパ腫の一例ポスター発表
- 第78回日本血液学会学術集会, 2016年10月, 日本語, 日本血液学会, 横浜, 国内会議The antiemetic effect of palonosetron in malignant lymphoma patients treated with the CHOP regimenポスター発表
- 第78回日本血液学会総会, 2016年10月, 日本語, 日本血液学会, 横浜, 国内会議Ph陽性慢性骨髄性白血病にみられた先天的バーキット様相互転座t(8;22)(q24;q11.2)ポスター発表
- 第78回 日本血液学会総会, 2016年10月, 日本語, 日本血液学会, 横浜, 国内会議Mycophenolate mofetil for GVHD prophylaxis might not favor the development of engraftment syndrome.ポスター発表
- 21st Congress of APBMT, 2016年10月, 英語, Asia-Pacific Blood and Marrow Transplantation Group, Singapore, Singapore, 国際会議LONG-TERM FOLLOW-UP OUTPATIENT PROGRAM AFTER HEMATOPOIETIC STEM CELL TRANSPLANTATION口頭発表(一般)
- 第78回日本血液学会学術集会, 2016年10月, 日本語, 日本血液学会, 横浜市, 国内会議Coexpression of NUP98/TOP1 and TOP1/NUP98 in de novo AML with t(11;20)(p15;q12) and t(2;5)(q33;q31)ポスター発表
- 第78回 日本血液学会総会, 2016年10月, 日本語, 日本血液学会, 横浜, 国内会議BRAF V600E mutation-specific antibody for the diagnosis of hairy cell leukemia .ポスター発表
- The 21st Annual Congress of Asia Pacific Blood and Marrow Transplantation Group 2016, 2016年10月, 英語, Asia-Pacific Blood and Marrow Transplantation Group, Singapore, Singapore, 国際会議A REDUCED INTENSITY CONDITIONING REGIMEN USING FLUDARABINE AND BUSULFAN (FLU-BU2)ポスター発表
- 第213回日本内科学会近畿地方会, 2016年09月, 日本語, 日本内科学会, 大阪, 国内会議リンパ節生検で完全梗塞が認められ診断に難渋したホジキンリンパ腫の1例口頭発表(一般)
- 第14回日本臨床腫瘍学会学術集会, 2016年07月, 日本語, 日本臨床腫瘍学会, 神戸, 国内会議発熱性好中球減少症における質量分析を用いた菌種同定の有用性についての検討口頭発表(一般)
- 第14回日本臨床腫瘍学会学術集会, 2016年07月, 日本語, 日本臨床腫瘍学会, 神戸, 国内会議同種造血幹細胞移植後のリンパ球回復と予後に関する後方視的解析口頭発表(一般)
- 第14回日本臨床腫瘍学会, 2016年07月, 日本語, 日本臨床腫瘍学会, 神戸市, 国内会議造血器悪性腫瘍に対する化学療法中のD-indexと口腔内感染性合併症との関連についての検討口頭発表(一般)
- 第14回日本臨床腫瘍学会学術集会, 2016年07月, 日本語, 日本臨床腫瘍学会, 神戸, 国内会議急性骨髄性白血病に対するヘッジホッグ阻害薬投与の治療反応性バイオマーカーとしてのNANOG発現口頭発表(一般)
- 第14回日本臨床腫瘍学会学術集会, 2016年07月, 英語, 日本臨床腫瘍学会, 神戸, 国内会議Treatment strategies for double primary neoplasm patients who develop malignant lymphoma and solid tumors口頭発表(一般)
- 第14回日本臨床腫瘍学会学術集会, 2016年07月, 日本語, 日本臨床腫瘍学会, 神戸, 国内会議Quick detection of bacteria with mass spectrometry might reduce mortality in ferile neutropenia口頭発表(一般)
- 第14回日本臨床腫瘍学会学術集会, 2016年07月, 日本語, 日本臨床腫瘍学会, 神戸, 国内会議Mycophenolate Mofetilを使用した造血幹細胞移植後に発症したHHV-6脳炎の治療経過口頭発表(一般)
- 7th, JSH International Symposium, 2016年05月, 英語, 日本血液学会, Awaji, Japan, 国際会議Delayed Absolute Lymphocyte Count Recovery after Allogeneic Hematopoietic Stem Cell Transplantation with Mycophenolate Mofetilポスター発表
- 42nd, Annual Meeting of the European Society for Blood and Marrow Transplantation, 2016年04月, 英語, European Society for Blood and Marrow Transplantation, Valencia, Spain, 国際会議Absolute Lymphocyte Count Recovery Predicts Clinical Outcome after Allogeneic Hematopoietic Stem Cell Transplantation in a Japanese Populationポスター発表
- 第38回日本造血幹細胞移植学会総会, 2016年03月, 日本語, 日本造血細胞移植学会, 名古屋, 国内会議自律神経ストームを伴った造血幹細胞移植後HHV-6脳炎口頭発表(一般)
- 第31回日本静脈経腸栄養学会学術集会, 2016年02月, 日本語, 福岡, 国内会議造血幹細胞移植患者への栄養管理の前後比較口頭発表(一般)
- 第19回日本病態栄養学会, 2016年01月, 日本語, 日本静脈経腸栄養学会, 横浜, 国内会議造血幹細胞移植患者への栄養管理の前後比較口頭発表(一般)
- 第31回日本静脈経腸栄養学会, 2016年01月, 日本語, 日本静脈経腸栄養学会, 横浜, 国内会議造血幹細胞移植患者へのNEST介入前後の栄養評価口頭発表(一般)
- 第19回日本病態栄養学会年次学術集会, 2016年01月, 日本語, 日本病態栄養学会, 横浜, 国内会議造血幹細胞移植患者へのNEST介入前後の栄養評価口頭発表(一般)
- 第210回日本内科学会近畿地方, 2015年11月, 日本語, 日本内科学会, 神戸, 国内会議超高齢で発症した発作性夜間血色素尿症の1例。口頭発表(一般)
- 第210回日本内科学会近畿地方, 2015年11月, 日本語, 日本内科学会, 神戸, 国内会議診断に難渋したhairy cell leukemiaの一例。口頭発表(一般)
- 第210回日本内科学会近畿地方, 2015年11月, 日本語, 日本内科学会, 神戸, 国内会議30年来頚部腫瘤を有する患者より発症したDouble-hit lymphoma(DHL)の1例口頭発表(一般)
- 第77回日本血液学会学術集会, 2015年10月, 英語, 日本血液学会, 金沢, 国内会議Mixed phenotype acute leukemia with t(12;17)(p13;q21)/TAF15-ZNF384 and der(1;18)(q10;q10)ポスター発表
- 第20回アジア太平洋造血細胞移植学会年次学術集会, 2015年10月, 英語, アジア太平洋造血細胞移植学会, 沖縄, 国際会議Herpesvirus 6 Encephalitis after Hematopoietic Stem Cell Transplantation with Administration of Mycophenolate Mofetil for Graft-versus-host Disease Prophylaxis in Japan: a Single Institution Experience.ポスター発表
- 第77回日本血液学会学術集会, 2015年10月, 英語, 日本血液学会, 金沢, 国内会議A case of scopulariopsis pneumonia with acute myeloid leukemiaポスター発表
- 第77回日本血液学会学術集会, 2015年10月, 英語, 日本血液学会, 金沢, 国内会議A case of myeloid sarcoma with myelofibrosis secondary to polycthemia veraポスター発表
- 第209回日本内科学会近畿地方会, 2015年09月, 日本語, 日本内科学会, 大阪, 国内会議血球貪食症候群を合併した脾臓原発びまん性大細胞型B細胞リンパ腫(DLBCL)の1例口頭発表(一般)
- 第7回日本静脈経腸栄養学会近畿支部学術集会, 2015年07月, 日本語, 京都, 国内会議造血幹細胞移植患者へのNST介入の評価口頭発表(一般)
- the 6th JSH International Symposium, 2015年05月, 英語, 日本血液学会, 軽井沢, 国内会議Absolute Lymphocyte Count Recovery Predicts Clinical Outcomes after Allogeneic Hematopoietic Stem Cell Transplantationポスター発表
- 第112回日本内科学会総会, 2015年04月, 日本語, 日本内科学会, 京都, 国内会議化学療法中の固形腫瘍患者に対するインフルエンザワクチン2回接種法の有効性と安全性.口頭発表(一般)
- 日本薬学会第135年会, 2015年03月, 日本語, 日本薬学会, 神戸, 国内会議造血幹細胞移植患者に対する栄養管理への介入とその評価ポスター発表
- 第38回日本造血幹細胞移植学会総会, 2015年03月, 日本語, 日本造血細胞移植学会, 名古屋, 国内会議骨髄非破壊的造血幹細胞移植後の非感染性心内膜炎口頭発表(一般)
- 第37回日本造血細胞移植学会総会, 2015年03月, 英語, 日本造血細胞移植学会, 神戸, 国内会議骨髄非破壊的造血幹細胞移植後の非感染性心内膜炎口頭発表(一般)
- 第54回 神戸血液病研究会, 2015年02月, 日本語, 神戸血液病研究会, 神戸, 国内会議急性骨髄性白血病に対して同種骨髄移植後にCandida Parapsilosis による横紋筋融解症を呈した1例口頭発表(一般)
- 56th Meeting of the American Society of Hematology, 2014年12月, 英語, American Society of Hematology, サンフランシスコ, 国際会議Efficacy of the Two-Dose Influenza Vaccine in Cancer Patients Receiving Chemotherapy: A Prospective Study.ポスター発表
- 第76回日本血液学会学術集会, 2014年10月, 日本語, 日本血液学会, 大阪, 国内会議肺、大腸重複癌に合併した自己免疫性溶血性貧血における自己抗体の標的分子の同定口頭発表(一般)
- 第76回日本血液学会学術集会, 2014年10月, 英語, 日本血液学会, 大阪, 国内会議MYC amplification in the form of double minute chromosomes in two cases of acute myeloid leukemia.ポスター発表
- 第76回日本血液学会学術集会, 2014年10月, 英語, 日本血液学会, 大阪, 国内会議A case of spontaneous hemophilia B diagnoses in adulthoodポスター発表