SEARCH
検索詳細
井上 潤医学部附属病院 光学医療診療部助教
研究活動情報
■ 受賞■ 論文
- 2019年02月, Biotechnol J, 英語Construction of a Model Culture System of Human Colonic Microbiota to Detect Decreased Lachnospiraceae Abundance and Butyrogenesis in the Feces of Ulcerative Colitis Patients[査読有り]研究論文（学術雑誌）
- Compositional alteration of the gut microbiota is associated with ulcerative colitis (UC). Here, a model culture system is established for the in vitro human colonic microbiota of UC, which will be helpful for determining medical interventions. 16S ribosomal RNA sequencing confirms that UC models are successfully developed from fecal inoculum and retain the bacterial species biodiversity of UC feces. The UC models closely reproduce the microbial components and successfully preserve distinct clusters from the healthy subjects (HS), as observed in the feces. The relative abundance of bacteria belonging to the family Lachnospiraceae significantly decreases in the UC models compared to that in HS, as observed in the feces. The system detects significantly lower butyrogenesis in the UC models than that in HS, correlating with the decreased abundance of Lachnospiraceae. Interestingly, the relative abundance of Lachnospiraceae does not correlate with disease activity (defined as partial Mayo score), suggesting that Lachnospiraceae persists in UC patients at a decreased level, irrespective of the alteration in disease activity. Moreover, the system shows that administration of Clostridium butyricum MIYAIRI restores butyrogenesis in the UC model. Hence, the model detects deregulation in the intestinal environment in UC patients and may be useful for simulating the effect of probiotics.2019年, Biotechnology Journal, 14(5) (5), e1800555, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- BACKGROUND AND AIMS: Proton pump inhibitors (PPIs) are among the most frequently prescribed medications. Side effects including an increased risk of intestinal infections have been reported. It is assumed that PPIs can increase susceptibility to enteropathogens; however, the underlying mechanisms are unknown. Here in this study, we explored whether Lansoprazole (Laz), one of the PPIs, increases the susceptibility to enteropathogens, and further investigated the mechanism of it. METHODS: Mice were administered Laz intraperitoneally once daily and orally infected with Citrobacter rodentium (C. rodentium). The establishment of intestinal infection was assessed by histology and inflammatory cytokine expression levels measured by quantitative PCR. To test whether Laz changes the intestinal environment to influence the susceptibility, intestinal pH, microbiota, metabolites and immune cell distributions were evaluated via pH measurement, 16S rRNA gene sequencing, metabolome, and flow cytometry analyses after Laz administration. RESULTS: Colitis was induced with less C. rodentium in Laz-treated mice as compared with the controls. We found that increased numbers of C. rodentium could reach the cecum following Laz administration. Laz increased pH in the stomach but not in the intestines. It induced dysbiosis and changed the metabolite content of the small intestine. However, these changes did not lead to alterations of immune cell distribution. CONCLUSIONS: Laz raised susceptibility to C. rodentium as increased numbers of the pathogen reach the site of infection. Our results suggest that it was due to increased stomach pH which allowed more peroral enteropathogens to pass the stomach, but not because of changes of intestinal environment.2018年04月, Digestive diseases and sciences, 63(4) (4), 881 - 889, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- 2015年03月, LIFE SCIENCES, 124, 31 - 40, 英語[査読有り]研究論文（学術雑誌）
- 2014年09月, Int J Med Sci, 11(12) (12), 1208 - 17, 英語Intestinal and hepatic expression of cytochrome P450s and mdr1a in rats with indomethacin-induced small intestinal ulcers[査読有り]研究論文（学術雑誌）
- 2014年, J Pharmacol Sci, 124(2) (2), 180 - 91, 英語Downregulation of CYP3A and P-glycoprotein in the secondary inflammatory response of mice with dextran sulfate sodium-induced colitis and its contribution to cyclosporine A blood concentrations[査読有り]研究論文（学術雑誌）
- 2011年06月, J Pharmacol Exp Ther, 337(3) (3), 838 - 45, 英語Mitochondria determine the efficacy of anticancer agents that interact with DNA but not the cytoskeleton[査読有り]研究論文（学術雑誌）
- 自己免疫性膵炎の診断基準2006の改定にともない限局性のAIPが数多く報告されるようになった．しかし，限局性のAIPは膵癌との鑑別が困難で，確定診断に苦慮する例がしばしば存在する．今回われわれは，鑑別診断が困難で膵切除術に至り術後に残膵に再燃をきたしたAIPの2症例を経験した．2症例はともに肺門部リンパ節腫大をともなっていた．AIPは全身性IgG4関連疾患であるという概念が広く認知され，膵外病変やステロイドの反応性を含めた診断基準の必要性も示唆された．一般財団法人 日本消化器病学会, 2011年, 日本消化器病学会誌, 108(4) (4), 640 - 649, 日本語[査読有り]研究論文（学術雑誌）
- 2011年, Rinsho Byori, 48(6) (6), 187 - 188, 日本語Oxidative stress and renal pathophysiology.[査読有り]研究論文（学術雑誌）
- 2011年01月, Photochem Photobiol, 87(1) (1), 191 - 8, 英語Ultraviolet-A irradiation to the eye modulates intestinal mucosal functions and properties of mast cells in the mouse[査読有り]研究論文（学術雑誌）
- 2010年12月, J Microbiol, 48(6) (6), 778 - 83, 英語Role of hydrogen generation by Klebsiella pneumoniae in the oral cavity[査読有り]研究論文（学術雑誌）
■ 講演・口頭発表等
- 第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議In vitro培養系ヒト腸内細菌叢モデルによる潰瘍性大腸炎患者の代謝プロファイル異常の検出ポスター発表
- 13th Congress of ECCO, 2018年02月, 英語, オーストリア, ウィーン, 国際会議Successful detection of dysbiosis and altered short-chain-fatty acids levels in in vitro colonic microbiota culture system using faecal samples of ulcerative colitisポスター発表
- 第8回日本炎症性腸疾患学会 学術集会, 2017年12月, 日本語, 日本炎症性腸疾患学会, 東京, 国内会議複数の腸炎モデルマウスに対する漢方薬青黛の効果の検討ポスター発表
- 25th United European Gastroenterology Week, 2017年10月, 英語, United European Gastroenterology, バルセロナ, スペイン, 国際会議Increased susceptibility to enteropathogenic bacteria by proton pump inhibitors in the murine model of food poisoningポスター発表