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近江戸 伸子大学院人間発達環境学研究科 人間環境学専攻教授
研究活動情報
■ 受賞- 2007年08月 日本植物細胞分子生物学会, 日本植物細胞分子生物学会技術賞, バイオテクノロジーを利用したフキならびにナスの育種
- 2007年08月 国際牧草分子生物学会, 第5回国際牧草分子生物学会ポスター賞, Chromosome map analysis of red clover
- 2002年03月 日本育種学会, 日本育種学会奨励賞, 超微細蛍光in situハイブリダイゼーション法の開発
- 2001年01月 財団法人日本染色体学会, 染色体学会賞, 高感度可視化法による植物染色体の分子細胞学的研究
- BACKGROUND AND AIMS: Ornamental hortensias are bred from a reservoir of over 200 species in the genus Hydrangea s.l. (Hydrangeaceae), and are valued in gardens, households and landscapes across the globe. The phenotypic diversity of hortensia cultivars, hybrids and wild relatives is mirrored by their genomic variation, with differences in genome size, base chromosome numbers and ploidy level. We aim to understand the genomic and chromosomal basis of hortensia genome variation. Therefore, we analysed six hortensias with different origins and chromosomal setups for repeatome divergence, the genome fraction with the highest sequence turnover. This holds information from the hortensias' evolutionary paths and can guide breeding initiatives. METHODS: We compiled a hortensia genotype panel representing members of the sections Macrophyllae, Hydrangea, Asperae and Heteromallae and reconstructed a plastome-based phylogenetic hypothesis as the evolutionary basis for all our analyses. We comprehensively characterized the repeatomes by whole-genome sequencing and comparative repeat clustering. Major tandem repeats were localized by multicolour FISH. KEY RESULTS: The Hydrangea species show differing repeat profiles reflecting their separation into the two major Hydrangea clades: diploid Hydrangea species from Japan show a conserved repeat profile, distinguishing them from Japanese polyploids as well as Chinese and American hortensias. These results are in line with plastome-based phylogenies. The presence of specific repeats indicates that H. paniculata was not polyploidized directly from the common ancestor of Japanese Hydrangea species, but evolved from a distinct progenitor. Major satellite DNAs were detected over all H. macrophylla chromosomes. CONCLUSIONS: Repeat composition among the Hydrangea species varies in congruence with their origins and phylogeny. Identified species-specific satDNAs may be used as cytogenetic markers to identify Hydrangea species and cultivars, and to infer parental species of old Hydrangea varieties. This repeatome and cytogenetics information helps to expand the genetic toolbox for tracing hortensia evolution and guiding future hortensia breeding.2025年02月, Annals of botany, 135(3) (3), 549 - 564, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Abstract Background and Aims Urbanization-induced environmental changes affect the geographical distribution of natural plant species. This study focused on how polyploidization, a dynamic genome change, influences the survival and distribution of Commelina communis (Cc) and its subspecies C. communis f. ciliata (Ccfc), which have variable chromosome numbers (e.g. Cc, 2n = 88 for Cc; Ccfc, 2n = 46 for Ccfc). The aim was to investigate polyploidization effects on natural plant distribution in urban environments. Methods The geographical distribution across urban–rural gradients was investigated at a total of 218 sites in Japan. Stomata size and density were measured and compared between Cc and Ccfc. Flow cytometry determined genome size and polyploidy. Chromosome karyotyping was performed using the genomic in situ hybridization (GISH) method. Key Results Urban areas were exclusively dominated by Cc, while Cc and Ccfc coexisted in rural areas. Cc had larger and fewer stomata and a genome size more than twice that of Ccfc. GISH results indicated that Cc possesses Ccfc and another unknown genome, suggesting allopolyploidy. Conclusions Our results show that the ploidy difference affects the geographical distribution, stomata traits and genome size between two distinct taxa in the genus Commelina, C. communis as a neo-tetraploid and C. communis f. ciliata, the diploid. Cc is an allopolyploid and is therefore not only polyploidy but also has an additional genome that provides new sets of genes and alleles, contributing to Cc having enhanced survival potentials in urban environments compared with Ccfc. This is the first investigation to clarify the distribution difference related to urban environments, the difference in stomata traits and genome size, and to study chromosome composition in Commelina species.Oxford University Press (OUP), 2024年08月, Annals of Botany[査読有り]研究論文（学術雑誌）
- Science Society of Thailand, 2024年04月, ScienceAsia, 50(3) (3), 1 - 1, 英語研究論文（学術雑誌）
- Topoisomerase II (TopoII) is an essential structural protein of the metaphase chromosome. It maintains the axial compaction of chromosomes during metaphase. It is localized at the axial region of chromosomes and accumulates at the centromeric region in metaphase chromosomes. However, little is known about TopoII localization and distribution in plant chromosomes, except for several publications. We used high voltage transmission electron microscopy (HVTEM) and ultra-high voltage transmission electron microscopy (UHVTEM) in conjunction with immunogold labeling and visualization techniques to detect TopoII and investigate its localization, alignment, and density on the barley chromosome at 1.4 nm scale. We found that HVTEM and UHVTEM combined with immunogold labeling is suitable for the detection of structural proteins, including a single molecule of TopoII. This is because the average size of the gold particles for TopoII visualization after silver enhancement is 8.9 ± 3.9 nm, which is well detected. We found that 31,005 TopoII molecules are distributed along the barley chromosomes in an unspecific pattern at the chromosome arms and accumulate specifically at the nucleolus organizer regions (NORs) and centromeric region. The TopoII density were 1.32-fold, 1.58-fold, and 1.36-fold at the terminal region, at the NORs, and the centromeric region, respectively. The findings of TopoII localization in this study support the multiple reported functions of TopoII in the barley metaphase chromosome.2024年04月, Micron (Oxford, England : 1993), 179, 103596 - 103596, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Abstract Urban development greatly alters the natural and semi‐natural habitats of native plants. Urbanisation results in a range of diverse habitats including remnant agricultural lands, urban parks, and roadside habitats. This habitat diversity often promotes trait divergence within urban areas. However, the mechanisms by which diverse urban habitats influence the population genetic structure of individual plant species remain poorly understood. We investigated the effects of urbanisation on genetic diversity and structure within 24 Commelina communis populations across diverse habitat types (rural agricultural land, urban agricultural land, urban park land, and urban roadsides) within the Kyoto–Osaka–Kobe megacity in Japan. We conducted multiplexed inter‐simple sequence repeat genotyping to compare genetic diversity among populations in different habitats. We also examined the correlation between Nei's genetic distance and geographic and environmental distances and performed principal coordinate analysis (PCoA) to evaluate genetic differentiation among urban habitats. There were no significant differences in genetic diversity indices between urban and rural populations and among urban habitat types. Although we detected no isolation‐by‐distance structure in population pairs of the same habitat type and in those of different habitats, the difference in surrounding landscape facilitated genetic differentiation not only between urban and rural habitats but also between different urban habitats. PCoA revealed no clear genetic differentiation among rural and urban habitat populations. Our findings indicate that the establishment of diverse habitat types through urbanisation has no and little impact on genetic diversity and structure, respectively, in C. communis, likely due to its high selfing rate and ability to adapt to urban conditions.Wiley, 2024年02月, Ecology and Evolution, 14(2) (2)[査読有り]研究論文（学術雑誌）
- 2023年12月, 日本科学教育学会研究会研究報告, 38(2) (2), 219 - 222, 日本語研究論文（研究会，シンポジウム資料等）
- Javaen barb fish Systomus orphoides Valenciennes, 1842 (Cypriniformes: Cyprinidae) is a freshwater fish whose population is declining and threatened with extinction. In this study, the ultrastructure of spermatozoa of Javaen barb fish (S. orphoides) was studied using transmission and scanning electron microscopy. The spermatozoa of S. orphoides are relatively simple cells composed of a spherical head, a short midpiece, and a flagellum, as in most Cyprinidae. The ultrastructure is characterized by the absence of acrosome, the total length of spermatozoa is 27.16 ± 4.5 μm, and the head has spherical with a length of 1.84 ± 0.10 μm and width of 1.55 ± 0.15 μm containing a nucleus, midpiece region containing the proximal and distal centrioles and mitochondria. Two or three mitochondria surrounding the axoneme (with a 9 + 2 microtubular pattern). Ultrastructural analyses by SEM and TEM of Javaen barb fish spermatozoa cells are very consistent with those of Cyprinidae. This study provides the ultrastructure information of S. orphoides spermatozoa in the Cyprinidae family this research could be useful in increasing reproductive efficiency and further prevent the extinction of this species.2023年11月, Microscopy research and technique, 86(11) (11), 1411 - 1415, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- The human genome is spatially and temporally organized in the nucleus as chromatin, and the dynamic structure of chromatin is closely related to genome functions. Cellular senescence characterized by an irreversible arrest of proliferation is accompanied by chromatin reorganisation in the nucleus during senescence. However, chromatin dynamics in chromatin reorganisation is poorly understood. Here, we report chromatin dynamics at the centromere region during senescence in cultured human cell lines using live imaging based on the clustered regularly interspaced short palindromic repeat/dCas9 system. The repetitive sequence at the centromere region, alpha-satellite DNA, was predominantly detected on chromosomes 1, 12, and 19. Centromeric chromatin formed irregular-shaped domains with high fluctuation in cells undergoing 5'-aza-2'-deoxycytidine-induced senescence. Our findings suggest that the increased fluctuation of the chromatin structure facilitates centromere disorganisation during cellular senescence.2023年09月, Scientific reports, 13(1) (1), 14609 - 14609, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- 2023年05月, Scientific reports, 13(1) (1), 8487 - 8487, 英語, 国際誌[査読有り]
- Recent evidence has demonstrated that specific epigenetic changes are also related to plant growth and development. Immunostaining enables the detection and characterization of chromatin modification, e.g., histone H4 acetylation (H4K5ac), histone H3 methylation (H3K4me2 and H3K9me2), and DNA methylation (5mC) with unique and specific patterns in plant tissues. Here we describe experimental procedures to determine the histone H3 methylation (H3K4me2 and H3K9me2) patterns in 3D-chromatin in whole roots tissue and 2D-chromatin in single nuclei in rice. To analyze both iron and salinity treatments, we show how to test for changes to the epigenetic chromatin landscape using heterochromatin (H3K9me2) and euchromatin (H3K4me) markers for chromatin immunostaining, especially in the proximal meristem region. To elucidate the epigenetic impact of environmental stress and external plant growth regulators, we demonstrate how to apply a combination of salinity, auxin, and abscisic acid treatments. The results of these experiments provide insights into the epigenetic landscape during rice root growth and development.2023年, Methods in molecular biology (Clifton, N.J.), 2672, 233 - 244, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- The higher order structure of the metaphase chromosome has been an enigma for over a century and several different models have been presented based on results obtained by a variety of techniques. Some disagreements in the results between methods have possibly arisen from artifacts caused during sample preparation such as staining and dehydration. Therefore, we treated barley chromosomes with ionic liquid to minimize the effects of dehydration. We also observed chromosomes on a film with holes to keep pristine chromosome structure from being flattened as seen when placed on a continuous support film. A chromosome placed over a hole in a thin carbon film was mounted on a tomography holder, and its structure was observed in three dimensions (3D) using electron tomography. We found that there are periodic structures with 300-400 nm pitch along the axis in barley chromosomes. The pitch sizes are larger than those observed in human chromosomes.Elsevier BV, 2022年09月, Micron, 160, 103328 - 103328, 英語[査読有り]研究論文（学術雑誌）
- Background: The use of image analysis to understand the structure of chromosome and chromatin is critical to the study of genetic evolution and diversification. Furthermore, a detailed chromosome map and the structure of chromatin in the nucleus may contribute to the plant breeding and the study of fundamental biology and genetics in organisms.Results: In plants with a fully annotated genome project, such as the Leguminosae species, the integration of genetic information, including DNA sequence data, a linkage map, and the cytological quantitative chromosome map could further improve their genetic value. The numerical parameters of chromocenters in 3D can provide useful genetic information for phylogenetic studies of plant diversity and heterochromatic markers whose epigenetic changes may explain the developmental and environmental changes in the plant genome. Extended DNA fibers combined with fluorescence in situ hybridization revealed the highest spatial resolution of the obtained genome structure. Moreover, image analysis at the nano-scale level using a helium ion microscope revealed the surface structure of chromatin, which consists of chromatin fibers compacted into plant chromosomes.Conclusions: The studies described in this review sought to measure and evaluate chromosome and chromatin using the image analysis method, which may reduce measurement time and improve resolution. Further, we discussed the development of an effective image analysis to evaluate the structure of chromosome and chromatin. An effective application study of cell biology and the genetics of plants using image analysis methods is expected to be a major propeller in the development of new applications.Engineering Sciences Press, 2022年04月, Quantitative Biology, 10(3) (3), 226 - 238, 英語[査読有り]研究論文（学術雑誌）
- In vivo monitoring of rice (Oryza sativa L.) seed germination and seedling growth under general conditions in closed Petri dishes containing agar base medium at room temperature (temperature = 24.5 ± 1 °C, relative humidity = 76 ± 7% (average ± standard deviation)), and induced degenerated callus formation with plant growth regulator, were performed using short-wavelength near-infrared spectroscopy and aquaphotomics over A period of 26 days. The results of spectral analysis suggest changes in water absorbances due to the production of common metabolites, as well as increases in biomass and the sizes of the samples. Quantitative models built to predict the day of the development provided better accuracy for rice seedlings growth compared to callus formation. Eight common water bands were identified as presenting prominent changes in the absorbance pattern. The water matrix of only rice seedlings showed three developmental stages: firstly expressing a predominantly weakly hydrogen-bonded state, then a more strongly hydrogen-bonded state, and then, again, a weakly hydrogen-bonded state at the end. In rice callus induction and proliferation, no similar change in water absorbance pattern was observed. The presented findings indicate the potential of aquaphotomics for the in vivo detection of degeneration in cell development.2021年09月, Plants (Basel, Switzerland), 10(9) (9), 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Cations, especially calcium ions (Ca2+), is one of the major factors responsible for the chromosome higher-order structure formation. The effects of cations on the human chromosomes have already been evaluated, however, whether the presence of similar effects on plant chromosomes has not been reported to date. Thus, in this study, we investigated the role of Ca2+ on the barley (Hordeum vulgare L.) chromosome structure. Barley chromosomes were isolated from the meristematic tissue within the germinated roots. The roots were subjected to enzymatic treatment, fixed, and drop on the cover glass to spread the chromosomes out. Some chromosomes were treated with BAPTA (1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid) to chelate Ca2+. Chromosome samples were then observed by fluorescence microscopy and scanning electron microscopy (SEM). The disperse structure of the chromosome was observed after BAPTA treatment. Chromosomes showed less condensed structure due to Ca2+ chelation. The high-resolution of SEM provided a more detailed visualization of chromosome ultrastructure under different calcium ion conditions. This study revealed the calcium ion effect on chromosome structure is important regardless of the organisms, suggesting a similar mechanism of chromosome condensation through humans and plants.2021年06月, Micron (Oxford, England : 1993), 145, 103046 - 103046, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Methylation systems have been conserved during the divergence of plants and animals, although they are regulated by different pathways and enzymes. However, studies on the interactions of the epigenomes among evolutionarily distant organisms are lacking. To address this, we studied the epigenetic modification and gene expression of plant chromosome fragments (~30 Mb) in a human-Arabidopsis hybrid cell line. The whole-genome bisulfite sequencing results demonstrated that recombinant Arabidopsis DNA could retain its plant CG methylation levels even without functional plant methyltransferases, indicating that plant DNA methylation states can be maintained even in a different genomic background. The differential methylation analysis showed that the Arabidopsis DNA was undermethylated in the centromeric region and repetitive elements. Several Arabidopsis genes were still expressed, whereas the expression patterns were not related to the gene function. We concluded that the plant DNA did not maintain the original plant epigenomic landscapes and was under the control of the human genome. This study showed how two diverging genomes can coexist and provided insights into epigenetic modifications and their impact on the regulation of gene expressions between plant and animal genomes.2021年05月, International journal of molecular sciences, 22(11) (11), 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Although plants and animals are evolutionarily distant, the structure and function of their chromosomes are largely conserved. This allowed the establishment of a human-Arabidopsis hybrid cell line in which a neo-chromosome was formed by insertion of segments of Arabidopsis chromosomes into human chromosome 15. We used this unique system to investigate how the introgressed part of a plant genome was maintained in human genetic background. The analysis of the neo-chromosome in 60- and 300-day-old cell cultures by next-generation sequencing and molecular cytogenetics suggested its origin by fusion of DNA fragments of different sizes from Arabidopsis chromosomes 2, 3, 4, and 5, which were randomly intermingled rather than joined end-to-end. The neo-chromosome harbored Arabidopsis centromeric repeats and terminal human telomeres. Arabidopsis centromere wasn't found to be functional. Most of the introgressed Arabidopsis DNA was eliminated during the culture, and the Arabidopsis genome in 300-day-old culture showed significant variation in copy number as compared with the copy number variation in the 60-day-old culture. Amplified Arabidopsis centromere DNA and satellite repeats were localized at particular loci and some fragments were inserted into various positions of human chromosome. Neo-chromosome reorganization and behavior in somatic cell hybrids between the plant and animal kingdoms are discussed.2021年03月, Scientific reports, 11(1) (1), 7160 - 7160, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- The chromosome compaction of chromatin fibers results in the formation of the nucleosome, which consists of a DNA unit coiled around a core of histone molecules associated with linker histone. The compaction of chromatin fibers has been a topic of controversy since the discovery of chromosomes in the 19th century. Although chromatin fibers were first identified using electron microscopy, the chromatin fibers on the surface of chromosome structures in plants remain unclear due to shrinking and breaking caused by prior chromosome isolation or preparation with alcohol and acid fixation, and critical point drying occurred into dehydration and denatured chromosomal proteins. This study aimed to develop a high-quality procedure for the isolation and preparation of plant chromosomes, maintaining the native chromosome structure, to elucidate the organization of chromatin fibers on the surface of plant chromosomes by electron microscopy. A simple technique to isolate intact barley (Hordeum vulgare) chromosomes with a high yield was developed, allowing chromosomes to be observed with a high-resolution scanning ion microscopy and helium ion microscopy (HIM) imaging technology, based on a scanning helium ion beam. HIM images from the surface chromatin fibers were analyzed to determine the size and alignment of the chromatin fibers. The unit size of the chromatin fibers was 11.6 ± 3.5 nm and was closely aligned to the chromatin network model. Our findings indicate that compacting the surface structure of barley via a chromatin network and observation via HIM are powerful tools for investigating the structure of chromatin.2021年03月, Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 29(1) (1), 81 - 94, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- It is well known that two DNA molecules are wrapped around histone octamers and folded together to form a single chromosome. However, the nucleosome fiber folding within a chromosome remains an enigma, and the higher-order structure of chromosomes also is not understood. In this study, we employed electron diffraction which provides a noninvasive analysis to characterize the internal structure of chromosomes. The results revealed the presence of structures with 100–200 nm periodic features directionally perpendicular to the chromosome axis in unlabeled isolated human chromosomes. We also visualized the 100–200 nm periodic features perpendicular to the chromosome axis in an isolated chromosome whose DNA molecules were specifically labeled with OsO4 using electron tomography in 300 keV and 1 MeV transmission electron microscopes.2021年02月, Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 27(1) (1), 149 - 155, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Divalent cations, mainly calcium and magnesium ions, are known to play a major role in the maintenance of chromosomes. The depletion of both ions using ethylenediaminetetraacetic acid (EDTA) results in a bent chromosome structure with extended arms and dispersed chromatin fibers. The importance of divalent cations for the maintenance of chromosome structure has been reported previously; nevertheless, previous studies were limited to qualitative data only. Straightening the bent image of the chromosome would provide quantitative data. Thus, this study aimed to evaluate the effects of cation depletion by the application of the Chromosome Image Analyzing System (CHIAS) to straighten bent chromosomes. Human HeLa chromosomes were treated with EDTA as a known chelating agent in order to investigate the importance of divalent cations on the maintenance of chromosome structure. Chromosomes were stained and directly observed with a fluorescence microscope. Images were then analyzed using CHIAS. The results revealed that EDTA-treated chromosomes showed longer arms than those without EDTA treatment, and most of them tended to bend-out. By straightening the image using CHIAS, the bent chromosomes were successfully straightened. The average lengths of the chromosomes treated with and without EDTA were 4.97 and 0.96 μm, respectively. These results signify the advantages of CHIAS for chromosome analysis and highlight the fundamental effects of cations on chromosome condensation.2020年11月, Microscopy research and technique, 83(11) (11), 1411 - 1416, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Cambridge University Press (CUP), 2020年07月, Microscopy and Microanalysis, 1 - 5[査読有り]研究論文（学術雑誌）
- The Oryza officinalis complex is the largest species group in Oryza, with more than nine species from four continents, and is a tertiary gene pool that can be exploited in breeding programs for the improvement of cultivated rice. Most diploid and tetraploid members of this group have a C genome. Using a new reference C genome for the diploid species O. officinalis, and draft genomes for two other C genome diploid species Oryza eichingeri and Oryza rhizomatis, we examine the influence of transposable elements on genome structure and provide a detailed phylogeny and evolutionary history of the Oryza C genomes. The O. officinalis genome is 1.6 times larger than the A genome of cultivated Oryza sativa, mostly due to proliferation of Gypsy type long-terminal repeat transposable elements, but overall syntenic relationships are maintained with other Oryza genomes (A, B, and F). Draft genome assemblies of the two other C genome diploid species, Oryza eichingeri and Oryza rhizomatis, and short-read resequencing of a series of other C genome species and accessions reveal that after the divergence of the C genome progenitor, there was still a substantial degree of variation within the C genome species through proliferation and loss of both DNA and long-terminal repeat transposable elements. We provide a detailed phylogeny and evolutionary history of the Oryza C genomes and a genomic resource for the exploitation of the Oryza tertiary gene pool.2020年04月, Genome biology and evolution, 12(4) (4), 413 - 428, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Abiotic stresses are non-living factors with negative morphological and physiological effects on living organisms. Substantial evidence exists that gene expression changes during plant cell growth are regulated by chromatin reconfiguration and histone modification. Several types of histone modifications are dramatically transformed in stress-responsive gene regions under drought stress conditions. Environmental stresses also cause the root apical meristem (RAM) region to decelerate root growth. In this study, we investigated how quantitative changes in epigenetic markers in this region influence rice morphology and physiology. Both iron and salinity treatments changed the epigenetic landscape from euchromatic to heterochromatic according to heterochromatin (H3K9me2) and euchromatin (H3K4me) markers, especially in the proximal meristem region. Moreover, supplementation with external abscisic acid (ABA) was able to mimic the effect of environmental stresses on global epigenetic changes. In contrast, the addition of external auxin (IAA) to rice under saline conditions affected heterochromatin formation without influencing euchromatin transformation. Chromatin dynamics is therefore believed to be directly connected to plant growth regulator signaling. We discuss insights into the role of plant growth regulators: ABA and IAA, peroxide signaling, and their effects on the global epigenetic change of histone modification under abiotic stresses.2019年12月, Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 27(4) (4), 287 - 298, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- The chromosome scaffold is considered to be a key structure of the mitotic chromosome. It plays a vital role in chromosome condensation, shaping the X-shaped structure of the mitotic chromosome, and also provides flexibility for chromosome movement during cell division. However, it remains to be elucidated how the chromosome scaffold organizes the mitotic chromosome and how it supports shaping the structure of the chromosome during metaphase. Here we present a new technique that enables the observation of the chromosome scaffold structure in metaphase chromosomes from any direction, by transferring an isolated chromosome to a 360° rotational holder for electron tomography (ET). The chromosome was stained with immunogold-labeled condensin complex, one of the major chromosome scaffold proteins and then observed in three dimensions using ET. Using the locations of gold nanoparticles to visualize the underlying structure, the tomograms we obtained reveal the patterns of chromosome scaffold organization, which appears to consist of a helical structure that serves to organize chromatin loops into the metaphase chromosome.2019年11月, Micron (Oxford, England : 1993), 126, 102736 - 102736, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- In sugarcane (Saccharum spp. hybrid) breeding, introgression of useful genes via intergeneric hybridization is a powerful strategy for improving the crop productivity. Erianthus arundinaceus shows great potential in terms of useful traits; however, little is known about the cytogenetic and agronomic characteristics of intergeneric hybrids between these two species. Here, we examine the cytogenetic and agronomic characteristics, and relationships between the two in intergeneric F1 hybrids between modern sugarcane cultivar and E. arundinaceus identified by amplification of 5S rDNA markers and morphological characteristics. The nuclear DNA content of the hybrids varied from 6.07 to 8.94 pg/2C, with intra-clonal variation in DNA content and 5S rDNA sites. Genomic in situ hybridization revealed 53 to 82 chromosomes in the hybrids, with 53 to 56 derived from sugarcane and 1 to 29 from E. arundinaceus. There were significant positive correlations between the number of E. arundinaceus chromosomes and dry matter yield, millable stalk weight, single stalk weight, and stalk diameter, but not sucrose content, reducing sugar content, sucrose/reducing sugar ratio or fiber content. This detailed information on intergeneric F1 hybrids between modern sugarcane cultivar and E. arundinaceus will contribute to effective utilization of E. arundinaceus in sugarcane breeding.Springer Nature Publishing AG, 2019年02月, Scientific reports, 9(1) (1), 1748 - 1748, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Spinach (Spinacia oleracea L.) is a dioecious plant with male heterogametic sex determination and homomorphic sex chromosomes (XY). The dioecism is utilized for producing commercial hybrid seeds, and hence understanding the molecular-genetic basis of the species' sex determining locus is an important issue for spinach breeding. In this study, seven dominant DNA markers were shown to completely co-segregate with the male-determining gene in segregating spinach populations comprising > 1500 plants. In addition, these seven dominant DNA markers were completely associated with the male-determining gene in over 100 spinach germplasm accessions and cultivars. These observations suggest that, in spinach, a Y-chromosomal region around the male-determining locus does not (or almost not) recombine with a counterpart region on the X chromosome. Using five of the seven DNA markers, five bacterial artificial chromosome (BAC) clone contigs with a total length of approximately 690 kbp were constructed. Full sequencing of six representative BAC clones (total insert length 504 kbp) from the five contigs and a transcriptome analysis by RNA-seq revealed that the Y-chromosomal region around the male-determining locus contains large amounts of repetitive elements, suggesting that the region might be poor in gene content. Most of the repeats found in this region are novel Ty1-copia-like and its derivative elements that accumulate predominantly in heterochromatic regions. Our findings may provide valuable insight into spinach genome structure and clues for future research into the evolution of the sex determining locus.Springer, 2018年04月, Molecular genetics and genomics : MGG, 293(2) (2), 557 - 568, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- A quantitative pachytene chromosome map of rice (Oryza sativa L.) was developed using imaging methods. The map depicts not only distribution patterns of chromomeres specific to pachytene chromosomes, but also the higher order information of chromosomal structures, such as heterochromatin (condensed regions), euchromatin (decondensed regions), the primary constrictions (centromeres), and the secondary constriction (nucleolar organizing regions, NOR). These features were image analyzed and quantitatively mapped onto the map by Chromosome Image Analyzing System ver. 4.0 (CHIAS IV). Correlation between H3K9me2, an epigenetic marker and formation and/or maintenance of heterochromatin, thus was, clearly visualized. Then the pachytene chromosome map was unified with the existing somatic chromosome and linkage maps by physically mapping common DNA markers among them, such as a rice A genome specific tandem repeat sequence (TrsA), 5S and 45S ribosomal RNA genes, five bacterial artificial chromosome (BAC) clones, four P1 bacteriophage artificial chromosome (PAC) clones using multicolor fluorescence in situ hybridization (FISH). Detailed comparison between the locations of the DNA probes on the pachytene chromosomes using multicolor FISH, and the linkage map enabled determination of the chromosome number and short/long arms of individual pachytene chromosomes using the chromosome number and arm assignment designated for the linkage map. As a result, the quantitative pachytene chromosome map was unified with two other major rice chromosome maps representing somatic prometaphase chromosomes and genetic linkages. In conclusion, the unification of the three rice maps serves as an indispensable basic information, not only for an in-depth comparison between genetic and chromosomal data, but also for practical breeding programs.PLOS, 2018年, PloS one, 13(4) (4), e0195710, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Chromosome instability of allopolyploid resynthesized Brassica napus.Allopolyploid resynthesized Brassica napus (AACC, 2n = 38) was produced by cross-hybridization between B. rapa (AA, 2n = 20) and B. oleracea (CC, 2n = 18) for new vegetative crop breeding. Many studies have provided evidences for the phenotype instability and close relationship between A and C genome in the amphidiploid resynthesized B. napus cultivars. In fact, a new B. napus2016年10月, Chromosome Science, 18, 79 - 84, 英語[査読有り]研究論文（国際会議プロシーディングス）
- Advances in cytology have led to the application of a wide range of visualization methods in plant genome studies. Image analysis methods are indispensable tools where morphology, density, and color play important roles in the biological systems. Visualization and image analysis methods are useful techniques in the analyses of the detailed structure and function of extended DNA fibers (EDFs) and interphase nuclei. The EDF is the highest in the spatial resolving power to reveal genome structure and it can be used for physical mapping, especially for closely located genes and tandemly repeated sequences. One the other hand, analyzing nuclear DNA and proteins would reveal nuclear structure and functions. In this chapter, we describe the image analysis protocol for quantitatively analyzing different types of plant genome, EDFs and interphase nuclei.2016年, Methods in molecular biology (Clifton, N.J.), 1469, 171 - 80, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- The dioecious genus Spinacia is thought to include two wild relatives (S. turkestanica Ilj. and S. tetrandra Stev.) of cultivated spinach (S. oleracea L.). In this study, nuclear and chloroplast sequences from 21 accessions of Spinacia germplasm and six spinach cultivars or lines were subjected to phylogenetic analysis to define the relationships among the three species. Maximum-likelihood sequence analysis suggested that the Spinacia plant samples could be classified into two monophyletic groups (Group 1 and Group 2): Group 1 consisted of all accessions, cultivars, and lines of S. oleracea L. and S. turkestanica Ilj. and two of five S. tetrandra Stev. accessions, whereas Group 2 was composed of the three remaining S. tetrandra Stev. accessions. By using flow cytometry, we detected a distinct difference in nuclear genome size between the groups. Group 2 also was characterized by a sexual dimorphism in inflorescence structure, which was not observed in Group 1. Interspecific crosses between the groups produced hybrids with drastically reduced pollen fertility and showed that the male is the heterogametic sex (XY) in Group 2, as is the case in S. oleracea L. (Group 1). Cytogenetic and DNA marker analyses suggested that Group 1 and Group 2 have homomorphic and heteromorphic sex chromosome pairs (XY), respectively, and that the sex chromosome pairs of the two groups evolved from a common ancestral pair. Our data suggest that the Spinacia genus may serve as a good model for investigation of evolutionary mechanisms underlying the emergence of heteromorphic sex chromosome pairs from ancestral homomorphic pairs.2015年06月, G3 (Bethesda, Md.), 5(8) (8), 1663 - 73, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- The mechanism whereby the same genome can give rise to different cell types with different gene expression profiles is a fundamental problem in biology. Chromatin organization and dynamics have been shown to vary with altered gene expression in different cultured animal cell types, but there is little evidence yet from whole organisms linking chromatin dynamics with development. Here, we used both fluorescence recovery after photobleaching and two-photon photoactivation to show that in stem cells from Arabidopsis thaliana roots the mobility of the core histone H2B, as judged by exchange dynamics, is lower than in the surrounding cells of the meristem. However, as cells progress from meristematic to fully differentiated, core histones again become less mobile and more strongly bound to chromatin. We show that these transitions are largely mediated by changes in histone acetylation. We further show that altering histone acetylation levels, either in a mutant or by drug treatment, alters both the histone mobility and markers of development and differentiation. We propose that plant stem cells have relatively inactive chromatin, but they keep the potential to divide and differentiate into more dynamic states, and that these states are at least in part determined by histone acetylation levels.AMER SOC PLANT BIOLOGISTS, 2014年12月, The Plant cell, 26(12) (12), 4821 - 33, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Attempts to elucidate chromosome structure have long remained elusive. Electron microscopy is useful for chromosome structure research because of its high resolution and magnification. However, biological samples such as chromosomes need to be subjected to various preparation steps, including dehydration, drying, and metal/carbon coating, which may induce shrinkage and artifacts. The ionic liquid technique has recently been developed and it enables sample preparation without dehydration, drying, or coating, providing a sample that is closer to the native condition. Concurrently, focused ion beam/scanning electron microscopy (FIB/SEM) has been developed, allowing the investigation and direct analysis of chromosome interiors. In this study, we investigated chromosome interiors by FIB/SEM using plant and human chromosomes prepared by the ionic liquid technique. As a result, two types of chromosomes, with and without cavities, were visualized, both for barley and human chromosomes prepared by critical point drying. However, chromosome interiors were revealed only as a solid structure, lacking cavities, when prepared by the ionic liquid technique. Our results suggest that the existence and size of cavities depend on the preparation procedures. We conclude that combination of the ionic liquid technique and FIB/SEM is a powerful tool for chromosome study.2014年10月, Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 20(5) (5), 1340 - 7, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- 2014年06月, Microsc Microanal., 10, 1 - 8, 英語Chromosome Interior Observation by Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM) Using Ionic Liquid Technique.[査読有り]研究論文（学術雑誌）
- Karyotyping using FISH and Chromosome Image Analysis System IV.In this study, kudzu (Pueraria lobata Ohwi), a new biomass plant widely grown in Japan, was investigated by chromosome karyotyping. Four strains of Japanese kudzu were examined, and all were found to contain 22 chromosomes. The 5S and 45S rDNA genes, which contain fundamental repeat sequences used in karyotyping, were determined by fluorescence in situ hybridization (FISH). OurSociety of Chromosome Research, 2014年02月, Chromosome Science, 16(1+2) (1+2), 17 - 21, 英語[査読有り]研究論文（学術雑誌）
- Gypsy-type retrotransposons comprise a large proportion of the plant genome. Identification and determination of their chromosomal distribution could contribute to a better understanding of the role and dynamics behind the repetitive elements in the genome and karyotype. It would also facilitate the selection of retrotransposon families for informative DNA markers. In the present study, we applied a PCR method by using degenerate oligonucleotide primers to isolate the reverse transcriptase (RT) region of gypsy-type retrotransposons in Jatropha curcas L., a biofuel crop. The analysis of 50 isolated PCR-amplified fragments showed a range of heterogeneity among predicted amino acid sequences. Comparative phylogenetic analyses of isolated RT fragments together with retrotransposon families from other plants allowed us to identify three families (Jg1-3) of gypsy-type retroelements in the jatropha genome. Jg1 and Jg2, having primer binding sites (PBS) complementary to tRNAArg, were found as jatropha-specific and belonged to the same lineage, which suggests that they arose during early evolution. On the other hand, Jg3 of a different lineage included elements of other species and had PBS complementary to tRNAMet. The computer-based data mining of jatropha whole genome allowed us to identify a high-copy number gypsy-type family Jg4 of the same lineage as Jg1 and Jg2 which had PBS complementary to tRNAArg. Furthermore, fluorescence in situ hybridization (FISH) analysis demonstrated that these gypsy-type elements are located in the pericentromeric region of jatropha chromosomes. The data are discussed within the context of the distinct dynamics of the gypsy-type retrotransposon families, their evolution, and their value for phylogenetic and biodiversity studies. © 2014 Springer Science+Business Media New York.Springer New York LLC, 2014年, Plant Molecular Biology Reporter, 32(4) (4), 923 - 930, 英語[査読有り]研究論文（学術雑誌）
- BACKGROUND: Recently, Jatropha curcas L. has attracted worldwide attention for its potential as a source of biodiesel. However, most DNA markers have demonstrated high levels of genetic similarity among and within jatropha populations around the globe. Despite promising features of copia-type retrotransposons as ideal genetic tools for gene tagging, mutagenesis, and marker-assisted selection, they have not been characterized in the jatropha genome yet. Here, we examined the diversity, evolution, and genome-wide organization of copia-type retrotransposons in the Asian, African, and Mesoamerican accessions of jatropha, then introduced a retrotransposon-based marker for this biofuel crop. RESULTS: In total, 157 PCR fragments that were amplified using the degenerate primers for the reverse transcriptase (RT) domain of copia-type retroelements were sequenced and aligned to construct the neighbor-joining tree. Phylogenetic analysis demonstrated that isolated copia RT sequences were classified into ten families, which were then grouped into three lineages. An in-depth study of the jatropha genome for the RT sequences of each family led to the characterization of full consensus sequences of the jatropha copia-type families. Estimated copy numbers of target sequences were largely different among families, as was presence of genes within 5 kb flanking regions for each family. Five copia-type families were as appealing candidates for the development of DNA marker systems. A candidate marker from family Jc7 was particularly capable of detecting genetic variation among different jatropha accessions. Fluorescence in situ hybridization (FISH) to metaphase chromosomes reveals that copia-type retrotransposons are scattered across chromosomes mainly located in the distal part regions. CONCLUSION: This is the first report on genome-wide analysis and the cytogenetic mapping of copia-type retrotransposons of jatropha, leading to the discovery of families bearing high potential as DNA markers. Distinct dynamics of individual copia-type families, feasibility of a retrotransposon-based insertion polymorphism marker system in examining genetic variability, and approaches for the development of breeding strategies in jatropha using copia-type retrotransposons are discussed.2013年09月, Biotechnology for biofuels, 6(1) (1), 129 - 129, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Vernalization, the promotion of flowering by cold, involves Polycomb-mediated epigenetic silencing of FLOWERING LOCUS C (FLC). Cold progressively promotes cell-autonomous switching to a silenced state. Here, we used live-cell imaging of FLC-lacO to monitor changes in nuclear organization during vernalization. FLC-lacO alleles physically cluster during the cold and generally remain so after plants are returned to warm. Clustering is dependent on the Polycomb trans-factors necessary for establishment of the FLC silenced state but not on LIKE HETEROCHROMATIN PROTEIN 1, which functions to maintain silencing. These data support the view that physical clustering may be a common feature of Polycomb-mediated epigenetic switching mechanisms.2013年09月, Genes & development, 27(17) (17), 1845 - 50, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- 2013年07月, Plant Image Analysis, 英語Advances in Imaging Methods on Plant Chromosomes.[査読有り]
- Springer New York, 2013年01月, Genetics and Genomics of Rice, 71 - 79, 英語[査読有り]論文集(書籍)内論文
- Red clover (Trifolium pratense L.) is a forage legume and an allogamous diploid plant (2n = 14; 440 Mb). Here, we examine the 7 prometaphase chromosomes of red clover using fluorescence in situ hybridization (FISH) with ribosomal RNA sequences, pericentromeric and telomeric repeats, as well as bacterial artificial chromosome (BAC) clones. Position of hybridization signals and chromosome condensation patterns were quantified by the help of the chromosome image analysis system ver. 4.0 (CHIAS IV). Fourteen BAC clones belonging to linkage groups (LG) 1-7 hybridized to individual chromosomes 4, 2, 6, 5, 1, 7, and 3, respectively. Quantitative analysis of FISH mapping and chromosome analysis using CHIAS IV allowed us to construct a quantitative idiogram that constitutes the comprehensive chromosome map of red clover. Chromosomal positions of the 26S rDNA locus were detected at a heterozygous locus on chromosome 6 in the variety HR, and polymorphisms of rDNA loci were observed in other varieties, although chromosomal positions of some BAC clones did not vary among HR and other varieties. These results demonstrate chromosomal collinearity among allogamous red clover varieties. This integration of genetic linkage and quantitative chromosome maps should provide valuable insight into allogamous legume genetics. Copyright (C) 2012 S. Karger AG, BaselKARGER, 2012年, CYTOGENETIC AND GENOME RESEARCH, 137(1) (1), 60 - 69, 英語[査読有り]研究論文（学術雑誌）
- Resynthesized Brassica napus cv. Hanakkori (AACC, 2n = 38) was produced by cross-hybridization between B. rapa (AA, 2n = 20) and B. oleracea (CC, 2n = 18) as a new vegetative crop. Many studies have provided evidences for the instability and close relationship between A and C genome in the resynthesized B. napus cultivars. In fact, seed produced to obtain progeny in Hanakkori had unstable morphological characters and generated many off-type plants. In this study, we investigated the pollen fertility, chromosome number, structure, and behavior linked to various Hanakkori phenotypes to define factors of unstable phenotypic expression in the progeny. Hanakkori phenotypes were categorized into five types. The results of pollen fertility, chromosome number, and fluorescence in situ hybridization analysis for somatic mitosis cells indicated that the off-type plants had lower pollen fertility, aberrant chromosome number, and structures with small chromosome fragments. Observation of chromosomes at meiosis showed that the meiotic division in off-type plants led to appreciably higher abnormalities than in on-type plants. However, polyvalent chromosomes were observed frequently in both on- and off-type plants in diplotene stage of meiosis. We assume that the unstable morphological characters in resynthesized progeny were the result of abnormal division in meiosis. It results as important that the plants of normal phenotype, chromosome structure and minimized abnormal meiosis are selected to stabilize progeny.SPRINGER, 2011年12月, THEORETICAL AND APPLIED GENETICS, 123(8) (8), 1433 - 1443, 英語[査読有り]研究論文（学術雑誌）
- The whole genome of Jatropha curcas was sequenced, using a combination of the conventional Sanger method and new-generation multiplex sequencing methods. Total length of the non-redundant sequences thus obtained was 285 858 490 bp consisting of 120 586 contigs and 29 831 singlets. They accounted for ~95% of the gene-containing regions with the average G + C content was 34.3%. A total of 40 929 complete and partial structures of protein encoding genes have been deduced. Comparison with genes of other plant species indicated that 1529 (4%) of the putative protein-encoding genes are specific to the Euphorbiaceae family. A high degree of microsynteny was observed with the genome of castor bean and, to a lesser extent, with those of soybean and Arabidopsis thaliana. In parallel with genome sequencing, cDNAs derived from leaf and callus tissues were subjected to pyrosequencing, and a total of 21 225 unigene data have been generated. Polymorphism analysis using microsatellite markers developed from the genomic sequence data obtained was performed with 12 J. curcas lines collected from various parts of the world to estimate their genetic diversity. The genomic sequence and accompanying information presented here are expected to serve as valuable resources for the acceleration of fundamental and applied research with J. curcas, especially in the fields of environment-related research such as biofuel production. Further information on the genomic sequences and DNA markers is available at http://www.kazusa.or.jp/jatropha/.2011年02月, DNA research : an international journal for rapid publication of reports on genes and genomes, 18(1) (1), 65 - 76, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- To construct a high-resolution pachytene chromosome map, we used the chromosome image analyzing system version 3 and fluorescence in situ hybridization. Two ribosomal RNA genes (45S rDNA and 5S rDNA), two major tandem repeat DNAs (LjTR1 and LjTR2), two major retroelements (LjRE1 and LjRE2), and 27 transformation-competent artificial chromosome clones were physically localized on Lotus japonicus (Miyakojima MG-20, 2n = 12) chromosomes. The distributions of heterochromatin and euchromatin along six chromosomes were compared based on the linkage map. Distortion between the recombination frequencies and physical chromosomal distance was recognized where the centromeric heterochromatic regions and constitutive heterochromatin are composed of the highest copy tandem repeat LjTR1 on the interstitial specific regions. Our study shows that the heterochromatin are composed of the specific repeated sequences, and the discrepancy between the recombination frequency and cytological information detected in L. japonicus chromosomes is due to the heterochromatin.2010年02月, Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 18(2) (2), 287 - 99, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- 2010年02月, Cytologia, vol.74 pp. 133-139, 133 - 139, 英語Physical mapping of ribosomal DNA genes on Jatropha curcas chromosomes by multicolor FISH.[査読有り]研究論文（学術雑誌）
- Small plant chromosomes show uneven condensation during the prometaphase stage in mitosis. Recently, an improved chromosome image analyzing system (CHIAS IV) has been released for measurement of the condensation patterns (CPs) in fluorescence images. CHIAS IV is based on the multiplatform public-domain image-processing software, ImageJ, which was developed by the National Institutes of Health, US. In this study, we used CHIAS IV to perform image analysis of the fluorescence patterns of somatic prometaphase chromosomes of the leguminous plant, red clover (Trifolium pratense L., 2n = 2x = 14). We produced a quantitative chromosome map of red clover by combining the qualitative-analysis results obtained using fluorescence in situ hybridization (FISH) mapping with the results of the CHIAS IV image-analysis method, thereby clearly demonstrating the effectiveness and utility of this software as an analytical tool for genetic and genomic studies.財団法人 染色体学会, 2009年07月, Chromosome science, 12(3) (3), 43 - 50, 英語
- 2008年11月, J. Biochem., vol. 143, pp. 325-332, 英語Anti-peptide antibodies for examining the conformation, molecular assembly, and localization of an intracellular protein, ribosormal protein S6, in vivo.[査読有り]研究論文（学術雑誌）
- The legume Lotus japonicus has been widely used as a model system to investigate the genetic background of legume-specific phenomena such as symbiotic nitrogen fixation. Here, we report structural features of the L. japonicus genome. The 315.1-Mb sequences determined in this and previous studies correspond to 67% of the genome (472 Mb), and are likely to cover 91.3% of the gene space. Linkage mapping anchored 130-Mb sequences onto the six linkage groups. A total of 10,951 complete and 19,848 partial structures of protein-encoding genes were assigned to the genome. Comparative analysis of these genes revealed the expansion of several functional domains and gene families that are characteristic of L. japonicus. Synteny analysis detected traces of whole-genome duplication and the presence of synteny blocks with other plant genomes to various degrees. This study provides the first opportunity to look into the complex and unique genetic system of legumes.2008年08月, DNA research : an international journal for rapid publication of reports on genes and genomes, 15(4) (4), 227 - 39, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Anti-peptide antibodies for examining the conformation, molecular assembly and localization of an intracellular protein, ribosomal protein S6, in vivo.Ribosomal protein S6 (rpS6) is known to relate to cell proliferation. Our recent proteome analysis of human metaphase chromosomes revealed the enrichment of rpS6 during mitosis. Here, structure, localization and molecular assembly in vitro and in vivo of a human rpS6, were examined using antibodies (Abs) prepared by immunizing rabbits with synthetic peptides. Five peptides, Ser6-Asp20 (S6-1), Ile52-Gly66 (S6-2), Asp103-Gly117 (S6-3), Asn146-Lys160 (S6-4) and Arg178-Ile192 (S6-5) were chosen as epitopes of human rpS6. These peptides except for S6-3 induced strong Ab production, and with an enzyme-linked immunosorbent assay, anti-S6-2, anti-S6-4 and anti-S6-5, showed high reactivity to recombinant rpS6 (r-rpS6), while anti-S6-1 did not, suggesting that S6-2, S6-4 and S6-5 were exposed on the r-rpS6 surface, while S6-1 was less exposed or possessed a different conformation. The immunostaining of HeLa cells as well as isolated chromosomes suggested that rpS6 occurs in endoplasmic reticulum (ER) but less possible on chromosomes since no Abs showed localization of rpS6 to chromosomes. In addition, the immunostaining suggested that only S6-4 is exposed on the protein surface, while S6-2 and S6-5 are buried by the interaction with other macromolecules in HeLa cells. Present our result shows new possibility of antibodies as tools for structure-oriented cell biology.2008年03月, Journal of biochemistry, 143(3) (3), 325 - 32, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Fluorescence in situ hybridization (FISH) using an Arabidopsis-type telomeric sequence (TTTAGGG)n probe to the mitotic chromosomes of Haplopappus gracilis (n=2) revealed the presence of Interstitial Telomere-like Repeats (ITRs) in at the subdistal position of the long arm of both chromosome pairs (1g and 2g). The H. gracilis genome (n=2) is generally thought to reconstitute from the n=4 complement of the allied species, H. ravenii. The sites we identified by FISH are in close proximity to the chromosomal rearrangement fusion points. This evidence supports the hypothesis that the karyotype of H. garacilis evolved from that of H. ravenii due to chromosome breakage and the subsequent end-to-end chromosome fusion. © 2007 The Japan Mendel Society.2007年12月, Cytologia, 72(4) (4), 483 - 488, 英語[査読有り]研究論文（学術雑誌）
- UV-irradiated cotyledonary protoplasts of Solanum integrifolium and iodoacetamide-treated cotyledonary protoplasts of S. sanitwongsei were electrofused and cultured. Regenerated plants were classified into three groups based on morphology and genomic in situ hybridization data. Morphology of the first group was intermediate between those of parental species. The plants bore fruits with viable seeds and had a chromosome number of 2n=48, the sum of the parental chromosome numbers, suggesting that they were symmetric fusion hybrids. Morphology of the plants in the second group was more S. integrifolium-like than that of the first group, and had two sets of S. integrifolium chromosomes and one set of S. sanitwongsei chromosomes. In contrast, plants in the third group had one set of S. integrifolium chromosomes and two sets of S. sanitwongsei chromosomes. Plants in the second and third groups were less vigorous than those in the first group, and bore few fruits. Electrophoretic analysis of the isozymes shikimate dehydrogenase, isocitrate dehydrogenase, and phosphoglucomutase, as well as random amplified polymorphic DNA analysis, demonstrated that 23 of regenerated plants from the three groups were somatic hybrids. The plants in the first group grew more vigorously than the parental plants and produced more than 5000 seeds per plant. The fertile somatic hybrids obtained in this study may be suitable candidates for eggplant rootstocks.JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY, 2007年03月, PLANT BIOTECHNOLOGY, 24(2) (2), 179 - 184, 英語[査読有り]研究論文（学術雑誌）
- Chromosome maps of legumes.Legumes are of immense importance as food and feed, and for sustainable agriculture, due to their ability to fix nitrogen. Here, the chromosome maps of the legumes soybean (Glycine max), Lotus (Lotus japonicus), and red clover (Trifolium pratense) are reviewed. These species have relatively small chromosomes and therefore are difficult to exploit for chromosome studies. Nevertheless, the identification of individual chromosomes became feasible, and chromosome maps have been developed applying image analysis and fluorescence in-situ hybridization. For Lotus japonicus, e.g. detailed chromosome maps have been developed using the information of genetic linkage maps. Future prospects of further legume chromosome mapping for breeding and genetic purposes are discussed.2007年, Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 15(1) (1), 97 - 103, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- 筆者らは,CSCLシステムであるKnowledge Forumを活用した小学生のための科学教育カリキュラムを開発・評価している.本研究では,遺伝子組換え食品問題に対する社会的意思決定を扱うカリキュラムを,知識構築に関するデザイン原則のひとつである認識主体性の観点から改善し,有効性を評価した.評価の指標は,この問題に対する個人的意見であり,単元開始時,中盤,終了後の3時点で測定し,学習の進行に伴う変化を改善前のカリキュラムでの結果と比較した.その結果,改善後のカリキュラムでは,単元の中盤から提案型の意見が出現し,単元後にはほぼ全員が,条件や代替策を示した賛成意見を記述した.児童の認識主体性を考慮した授業デザインの変更により,共同体にとって価値あるアイディアの創出という知識構築のレベルを,さらに向上させることができた.一般社団法人日本科学教育学会, 2006年08月, 日本科学教育学会研究会研究報告, 21(1) (1), 83 - 88, 日本語研究論文（研究会，シンポジウム資料等）
- 「市民の科学に対する大学の支援に関する実践的研究」(略称「市民の科学」)プロジェクトの一環として、「サイエンスカフェ神戸」を創始した。このプロジェクトは、科学技術的課題に対する市民のエンパワーメント・システム構築をめざすもので、サイエンスカフェ開催はその第一段階として位置づけられる。2005年10月から2006年6月までに16回を開催し、科学コミュニケーションの新しいスタイルとして高い可能性を確認した。サイエンスカフェは現在各地に広がりつつあるが、「サイエンスカフェ神戸」では、文化としての科学を地域社会に根づかせることを大きな目的とし、運営に市民が主体的に参加し、様々な場で頻繁に開催されるようなあり方をゴールとして設定している点で特徴をもっている。「市民の科学」プロジェクトでは、次のステップとして、サイエンスカフェを通じて形成された緩やかなネットワークも利用しつつ、大学の支援のもとでの、環境などに関わる課題の市民による調査・研究の展開可能性を探ってゆく。一般社団法人 日本科学教育学会, 2006年08月, 日本科学教育学会研究会研究報告, Vol. 21, No. 1, pp. 37-42(1) (1), 37 - 42, 日本語研究論文（学術雑誌）
- マメ科植物を対象としたゲノム解析の一環として、アカクローバ（Trifolium pretense L.）のゲノム構造情報を収集した。まず、フローサイトメトリーと顕微鏡観察により、ゲノムサイズの推定（約400 Mb）と核型分析（2n=14）日本植物生理学会, 2006年, 日本植物生理学会年会およびシンポジウム 講演要旨集, 2006(0) (0), 878 - 878
を行った。さらに、ランダムシークエンシングによってゲノムのGC含量を推定（34.2％）した。また、実生に由来する26000余りのESTを収集し、9000余りのnon-redundant 配列を得た。以上の基礎データに基づいて、マイクロサテライトマーカーの開発と高密度連鎖地図の作製を行った。マッピング集団はスイス及び日本の品種由来のクローン(HR)とロシア由来のクローバ（R130）の交配によるF1集団を用いた。ゲノムおよびcDNAに由来する4種類のライブラリーから83172クローンをランダムシークエンシングし29329 （35%）にSSR を認めた。このうち7244についてSSRを増幅するプライマーを合成しPCRを行ったところ、約1500で明確な多型が観察された。これらについてF1世代188個体をタイピングしJoinMap3.0を用いて連鎖地図を作製した結果、1286個のマイクロサテライトマーカーと148個の既存のRFLPマーカーからなる高密度連鎖地図完成した。本発表では、以上の解析データの詳細と比較ゲノム解析の結果を報告する。 - Springer International Publishing, 2006年, Biotechnology in Agriculture and Forestry, 58, 51 - 63, 英語[査読有り]研究論文（学術雑誌）
- RAPD markers encoding retrotransposable elements are linked to the male sex in Cannabis sativa L.Male-associated DNA sequences were analyzed in Cannabis sativa L. (hemp), a dioecious plant with heteromorphic sex chromosomes. DNA was isolated from male and female plants and subjected to random amplified polymorphic DNA analysis. Of 120 primers, 17 yielded 400 to 1500-bp fragments detectable in male, but not female, plants. These fragments were cloned and used as probes in gel-blot analysis of genomic DNA. When male and female DNA was hybridized with 2 of these male-specific fragments, MADC(male-associated DNA sequences in C. sativa)3 and MADC4, particularly intense bands specific to male plants were detected in addition to bands common to both sexes. The MADC3 and MADC4 sequences were shown to encode gag/pol polyproteins of copia-like retrotransposons. Fluorescence in situ hybridization with MADC3 and MADC4 as probes revealed a number of intense signals on the Y chromosome as well as dispersed signals on all chromosomes. The gel-blot analysis and fluorescence in situ hybridization results presented here support the hypothesis that accumulation of retrotransposable elements on the Y chromosome might be 1 cause of heteromorphism of sex chromosomes.2005年10月, Genome, 48(5) (5), 931 - 6, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- 科学・技術が高度に発達した社会において、(a)環境問題等の解決手段として、(b)知的探求活動として、市民の科学・技術にかかわる問題の調査・研究能力を高めてゆくこと(エンパワーメント)が大きな意味を持つ。我々は、神戸大学大学院総合人間科学研究科に設置された発達支援インスティテュート/ヒューマン・コミュニティ創成研究センターの研究プロジェクトとして「市民科学に対する大学の支援に関する実践的研究」の取り組みを始めた。本プロジェクトは、神戸を主なフィールドとして、幅広い年齢や素養をもつ市民が、大学の支援のもとに、科学リテラシーを高めるとともに、自らが調査・研究能力を獲得してゆく持続可能なシステムとそれを担う組織、人材のあり方を実践的に探り、日本の社会に適したモデルを構築することを目指す。一般社団法人 日本科学教育学会, 2005年09月, 科教研報, 20・2, 47-51(2) (2), 47 - 51, 日本語研究論文（学術雑誌）
- 筆者らは, CSCLシステムであるKnowledge Forumを小学校へ導入し, 遺伝子組換え食品問題に対する社会的意思決定をテーマとした, 小学生のための科学教育カリキュラムを開発・改善している.本研究では, 改善したカリキュラムの有効性を評価する一環として, 単元開始時, 中盤, 終了後の3時点で, 遺伝子組換え食品問題に対する個人的意見を記述させ, 学習の進行に伴う変化を検討した.その結果, 学習の進行に伴って賛否の立場の分布が変容したことに加え, その理由の記述が, イメージによるものから, エビデンスに基づくものや提案型のものへと変化したことが明らかになった.カリキュラムの目標である社会的意思決定の能力を獲得したことにより, 学習者は, 論争性の把握や対立の解消が明示的に要求されない場面においても, 賛否両論のエビデンスやそのバランスを考慮して, 建設的な意見を表明できるようになったといえる.一般社団法人 日本科学教育学会, 2005年09月, 日本科学教育学会研究会研究報告, 20・2, p1-p6(2) (2), 1 - 6, 日本語研究論文（学術雑誌）
- 筆者らは, CSCLシステムのKnowledge Forumを活用し, 遺伝子組換え食品問題に対する社会的意思決定をテーマとした科学教育カリキュラムを開発・改善している.2004年度版カリキュラムは, 2003年度版カリキュラムの評価結果で指摘された問題点を改善したものであった.本研究では, 改善したカリキュラム評価の一環として, 概念的理解とイメージの変容に関する評価結果を年度間で比較した.その結果, 遺伝子組換え食品に関する社会的な論争性の理解を深めるとともに, 遺伝子組換え食品に対するネガティブなイメージをポジティブとネガティブが混在するイメージへ変容させる点において, 2004年度版カリキュラムの高い効果が認められた.一般社団法人 日本科学教育学会, 2005年08月, 日本科学教育学会研究会研究報告, 20・1, p15-p20(1) (1), 15 - 20, 日本語研究論文（学術雑誌）
- Chromosomes are super-molecules consisting of DNA, histone and chromatin proteins, which specifically appear within a cell at the cell division. We analyzed barley chromosomes by atomic force microscopy (AFM) to elucidate its structural basis. Mitotic chromosomes were taken from root tips of barley (Hordeum vulgare L., cv. Minorimugi) using the EMA (enzymatic maceration and air-drying) method after synchronization of cell cycle. Both the air-dried or critical point dried specimens were observed in air by a dynamic force mode. This observation technique enables to obtain three-dimensional image data on the surface structure of barley chromosomes at high resolution without any metal coating. The details of the higher order chromosomal structure such as chromatin fibers were clarified with the biological significance. Acidic treatment (e.g., acetic acid treatment) for removing proteins was useful to obtain clear images of basic chromosomal structure. Thus, it is concluded that the AFM has a great potential for investigation of molecular structures of chromosomes. © 2005 The Japan Mendel Society.2005年03月, Cytologia, 70(1) (1), 101 - 108, 英語[査読有り]研究論文（学術雑誌）
- 筆者らは,CSCLシステムのKnowledge Forumを小学校へ導入し,遺伝子組換え食品問題に対する社会的意思決定をテーマとした小学生のための科学教育カリキュラムを開発・改善している.2004年度は,基礎的内容に関する理解促進および社会的意思決定の洗練という観点から,2003年度版カリキュラムを改善している.実験授業を実施し,学習者の社会的意思決定を適切性の観点から評価したところ,2004年度の達成度が2003年度よりも向上したことがわかった.一般社団法人 日本科学教育学会, 2005年, 日本科学教育学会年会論文集, 29(0) (0), 527 - 528, 日本語研究論文（学術雑誌）
- 筆者らは,遺伝子組換え食品問題に対する社会的意思決定をテーマとした小学生のための科学教育カリキュラムを開発している.本研究では,このカリキュラムの評価の一環として,育成した社会的意思決定の力,すなわち賛否両論を踏まえ,そのバランスを考慮しながらコンセンサスを創出する能力が,他の科学技術問題に転移するかどうか検討した.カリキュラムの前後で,原子力発電に関する社会的意思決定を学習者に課し,記述の内容を比較した結果,転移が認められ,特にコンセンサスの観点で向上が見られた.一般社団法人 日本科学教育学会, 2005年, 日本科学教育学会年会論文集, 29(0) (0), 525 - 526, 日本語研究論文（学術雑誌）
- Comprehensive structural analysis of the genome of red clover (Trifolium pratense L.).With the aim of establishing the basic knowledge and resources needed for applied genetics, we investigated the genome structure of red clover Trifolium pratense L. by a combination of cytological, genomic and genetic approaches. The deduced genome size was approximately 440 Mb, as estimated by measuring the nuclear DNA content by flow cytometry. Seven chromosomes could be distinguished by microscopic observation of DAPI stained prometaphase chromosomes and fluorescence in situ hybridization using 28S and 5S rDNA probes and bacterial artificial chromosome probes containing microsatellite markers with known positions on a genetic linkage map. The average GC content of the genomes of chloroplast, mitochondrion and nucleus were shown to be 33.8, 42.9 and 34.2%, respectively, by the analysis of 1.4 Mb of random genomic sequences. A total of 26,356 expressed sequence tags (ESTs) that were grouped into 9339 non-redundant sequences were collected, and 78% of the ESTs showed sequence similarity to registered genes, mainly of Arabidopsis thaliana and rice. To facilitate basic and applied genetics in red clover, we generated a high-density genetic linkage map with gene-associated microsatellite markers. A total of 7159 primer pairs were designed to amplify simple sequence repeats (SSRs) identified in four different types of libraries. Based on sequence similarity, 82% of the SSRs were likely to be associated with genes. Polymorphism was examined using two parent plants, HR and R130, and 10 F(1) progeny by agarose gel electrophoresis, followed by genotyping for the primer pairs showing polymorphisms using 188 F(1) plants from the mapping population. The selected 1305 microsatellite markers as well as the previously developed 167 restriction fragment length polymorphism markers were subjected to linkage analysis. A total of 1434 loci detected by 1399 markers were successfully mapped onto seven linkage groups totaling 868.7 cM in length; 405 loci (28%) were bi-parental, 611 (43%) were specific to HR and 418 (29%) were specific to R130. Each genetic linkage group was linked to a corresponding chromosome by FISH analysis using seven microsatellite markers specific to each of the linkage groups as probes. Transferability of the developed microsatellite markers to other germplasms was confirmed by testing 268 selected markers on 88 red clover germplasms. Macrosynteny at the segmental level was observed between the genomes of red clover and two model legumes, Lotus japonicus and Medicago truncatula, strongly suggesting that the genome information for the model legumes is transferable to red clover for genetic investigations and experimental breeding.2005年, DNA research : an international journal for rapid publication of reports on genes and genomes, 12(5) (5), 301 - 64, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- 筆者らは, CSCLシステムのKnowledge Forumを導入し, 遺伝子組換え食品問題に対する社会的意思決定をテーマとした小学生のための科学教育カリキュラムを開発している.2004年度は, 基礎的内容に関する理解の促進, および社会的意思決定の洗練という観点から, 昨年度のカリキュラムを改善した.基礎的内容に関する理解度を評価した結果, 昨年よりも理解度が向上したことが明らかになった.一般社団法人 日本科学教育学会, 2005年01月, 日本科学教育学会研究会研究報告, 19・3, p33-p38(3) (3), 33 - 38, 日本語研究論文（学術雑誌）
- 2005年01月, Genes Genet. Syst., 2: 35-39Fluorescent labeling of plant chromosomes in suspension by FISH.[査読有り]研究論文（学術雑誌）
- 2004年11月, Methods in Cell Science, 英語Chromosome painting as a tool for rice genetics and breeding.
- 2004年10月, 日本科学教育学会研究会研究報告, 19・1, p39-p44, 427 - 428, 日本語遺伝子組み換え食品問題に対する社会的意思決定をテーマとした科学教育のためのCSCL環境：単元目標の達成の評価研究論文（学術雑誌）
- 本研究では,神戸大学発達科学部附属住吉小学校をフィールドとして展開されているCSCLシステムを利用した授業のデザイン実験の実際について,Knowledge Forum,再構成型コンセプトマップ作成ソフトウェア「あんどう君」,ケータイ利用の野外情報共有システム(clippic)の事例を公開授業として報告する.一般社団法人 日本科学教育学会, 2004年10月, 日本科学教育学会研究会研究報告, 19・1, p1-p4(1) (1), 1 - 4, 日本語研究論文（学術雑誌）
- 一般社団法人日本科学教育学会, 2004年07月, 年会論文集, 28, 427 - 428, 日本語6I7-13 遺伝子組み換え食品問題に対する社会的意思決定をテーマとした科学教育のためのCSCL環境 : 社会的意思決定の達成度に関する分析(科学認識(1))研究論文（学術雑誌）
- 一般社団法人日本科学教育学会, 2004年07月, 年会論文集, 28, 425 - 426, 日本語6I7-12 遺伝子組み換え食品問題に対する社会的意思決定をテーマとした科学教育のためのCSCL環境:概念的理解とイメージの変容(科学認識(1))研究論文（学術雑誌）
- 一般社団法人日本科学教育学会, 2004年07月, 年会論文集, 28, 423 - 424, 日本語6I7-11 遺伝子組み換え食品問題に対する社会的意思決定をテーマとした科学教育のためのCSCL環境 : 基礎的内容の理解度(科学認識(1))研究論文（学術雑誌）
- The bio-active beads system has been verified as a novel transformation method. To enhance its versatility, we applied this method to the transformation of a monocot plant, rice. The transient GFP expression was observed 24 h after transformation. This indicates that the method can also be applied to monocotyledons.Japanese Society for Plant Cell and Molecular Biology, 2004年, Plant Biotechnology, 21(4) (4), 303 - 306, 英語[査読有り]研究論文（学術雑誌）
- it is very difficult to obtain interspecific hybrids between Nicotiana tabacum L. (2n = 48) and N. gossei Domin (2n = 36), because of strong cross incompatibility. We had already obtained interspecific hybrids between these two species, crossing N. gossei flower with N. tabacum pollen exposed to He ions or gamma-rays. Here, we analyze chromosome constitution of these hybrids by genomic in situ hybridization. In root tip cells of the two hybrids obtained with He ion exposure, most mitotic cells contained 18 chromosomes of N. gossei and 24 chromosomes of N. tabacum. However, in some cells, translocations and insertions between parental genomes were observed. On the other hand, in a hybrid obtained by gamma-ray irradiation, intergenomic rearrangements were not observed, although mitotic cells showed 19 hybridization signals with N. gossei DNA in 41 chromosomes. Such chromosomal changes in structure or constitution may be related to overcoming cross incompatibility between these two species. (C) 2003 Elsevier Science B.V. All rights reserved.ELSEVIER SCIENCE BV, 2003年05月, NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 206, 548 - 552, 英語[査読有り]研究論文（学術雑誌）
- Development of a quantitative pachytene chromosome map in Oryza sativa by imaging methodsA higher GC content region of an Oryza sativa chromosome can be specifically visualized by double staining with propidium iodide (PI) and 4, 6-diamidino-2-phenylindole (DAPI). This procedure allows identification of chromosome 9 from the other rice chromosomes at the pachytene stage. Using rice chromosome 9 as a model, an imaging method to construct a pachytene chromosomal map was developed by quantifying the fluorescence profile (FP) of each chromomere. The pachytene map of chromosome 9 consists of twenty-two chromomeres including four chromomeres within the nucleolar organizing region (NOR) and satellite region. The pachytene map was compared with the corresponding somatic prometaphase map and the linkage map. The differences among the three maps indicate that each map depicts specific biological information, which is difficult to be substituted by the other maps.GENETICS SOC JAPAN, 2003年04月, GENES & GENETIC SYSTEMS, 78(2) (2), 155 - 161, 英語[査読有り]研究論文（学術雑誌）
- Chinese Academic Publishers, Beijin, 2002年03月, Advances in Chromosome Sciences, 英語High resolution FISH for gene mapping and molecular analysis of rice chromosomes.
- To elucidate the evolutionary dynamics of rDNA site number in polyploid plants, we determined 5S and 18S-5.8S-26S rDNA sites for ten species of Sanguisorba (2n=14, 28, 56) and a single species of each of three outgroup genera, Agrimonia (2n=28), Rosa (2n=14), and Rubus (2n=14) by the fluorescence in situ hybridization (FISH) method. We also estimated phylogenetic relationships among these species using matK chloroplast DNA (cpDNA) sequences, and reconstructed the evolutionary history of rDNA site number based on the maximum parsimony method. The 2n=14 and 2n=28 plants of all genera except Rosa carried two 5S rDNA sites, whereas Rosa and 2n=56 plants carried four sites. The 2n=14 plants had two 18S-5.8S-26S rDNA sites, whereas Sanguisorba annua and 2n=28 plants had four or six sites. Phylogenetic analysis showed that polyploidization from 2n=14 to 2n=28 has occurred once or three times in Sanguisorba and Agrimonia. The 5S rDNA sites duplicated during each ancestral polyploidization were evidently lost after each polyploidization. However, the duplicated 18S-5.8S-26S rDNA sites were all conserved after each polyploidization. Thus, the duplicated 5S rDNA sites tend to have been eliminated, whereas those of 18S-5.8S-26S rDNA tend to have been conserved in Sanguisorba. In the most parsimonious hypothesis, 2n=14 in S. annua is a secondary, putatively dysploid state, reduced from 2n=28.Springer Verlag, 2002年, Chromosoma, 110(8) (8), 550 - 558, 英語[査読有り]研究論文（学術雑誌）
- EATON PUBLISHING CO, 2001年08月, BIOTECHNIQUES, 31(2) (2), 246 - +, 英語Rapid DNA fiber technique for size measurements of linear and circular DNA probes[査読有り]研究論文（学術雑誌）
- A new gypsy-type retrotransposon, RIRE7: preferential insertion into the tandem repeat sequence TrsD in pericentromeric heterochromatin regions of rice chromosomesA portion of an insertion sequence present in a member of the RIRE3 family of retrotransposons in Oryza sativa L. cv. IR36 was found to have an LTR sequence following by PBS sequence complementary to the 3 ' -end region of tRNA(Met), indicative of another rice retrotransposon (named RIRE7). Cloning and sequencing of PCR-amplified fragments that made up all parts of the RIRE7 sequence showed that RIRE7 is a gypsy-type retrotransposon with partial homology in the pol region to the rice gypsy-type retrotransposons RIRE2 and RIR3 identified in rice previously. Interestingly, various portions of the RIRE7 sequence were homologous to several DNA segments present in the centromere regions of cereal chromosomes. Further cloning and nucleotide sequencing of fragments flanking RIRE7 copies showed that RIRE7 was inserted into a site within a tandem repeat sequence that has a unit length of 155 bp. The tandem repeat sequence, named TrsD, was homologous to tandem repeat sequences RCS2 and CentC, previously identified in the centromeric regions of rice and maize chromosomes. Fluorescence in situ hybridization (FISH) analysis of the metaphas chromosomes of O. sativa cv. Nipponbare showed that both RIRE7 and TrsD sequences were present in the centromere regions of the chromosomes. The presence of RIRE7 and the TRsD sequences in the centromere regions of several chromosomes was confirmed by the identification of several YAC clones whose chromosomal locations are known. Further FISH analysis of rice pachytene chromosomes showed that the TrsD sequences were located in a pericentromeric heterochromatin region. These findings strongly suggest that RIRE7 and TrsD are components of the pericentromeric heterochromatin of rice chromosomes.SPRINGER-VERLAG, 2001年05月, MOLECULAR GENETICS AND GENOMICS, 265(3) (3), 480 - 488, 英語[査読有り]研究論文（学術雑誌）
- Karyotype analysis of Nicotiana kawakamii Y. Ohashi using DAPI banding and rDNA FISHPrometaphase cells were used to analyze the karyotype of Nicotiana: kawakamii Y. Ohashi by means of sequential Giemsa/CMA/DAPI staining and multicolor fluorescence in situ hybridization with 5S and 18S rDNA. Observation of the DAPI-stained prometaphase spreads indicated that N. kawakamii had six pairs of large chromosomes, one pair of medium-sized chromosomes and five pairs of small chromosomes. The six pairs of large chromosomes possessed remarkable DAPI bands, and each could be identified from both the DAPI banding pattern and the length of the short arm. The DAPI banding pattern was approximately identical to the CMA and Giemsa banding patterns. Hybridization signals of the 18S rDNA probe were detected on two pairs of large chromosomes. In addition, two pairs of small chromosomes were identified based on the position of the 5S rDNA signals. An idiogram of N. kawakamii chromosomes was produced based on DAPI bands and rDNA loci.SPRINGER-VERLAG, 2001年05月, THEORETICAL AND APPLIED GENETICS, 102(6-7) (6-7), 810 - 814, 英語[査読有り]研究論文（学術雑誌）
- 2001年05月, Chromosome Sci., 5, 174p-179p, 英語High resolution physical mapping of genes of plant chromosomes研究論文（学術雑誌）
- 2001年02月, Ann.Rep.IC Biotech., 英語Visualization of specific DNA sequences on chromosomes and extended DNA fibers.
- A new gypsy-type retrotransposon, RIRE7: Preferential insertion into the tandem repeat sequence TrsD in pericentromeric heterochromatin regions of rice chromosomesA portion of an insertion sequence present in a member of the RIRE3 family of retrotransposons in Oryza sativa L. cv. IR36 was found to have an LTR sequence followed by a PBS sequence complementary to the 3′-end region of tRNAMet, indicative of another rice retrotransposon (named RIRE7). Cloning and sequencing of PCR-amplified fragments that made up all parts of the RIRE7 sequence showed that RIRE7 is a gypsy-type retrotransposon with partial homology in the pol region to the rice gypsy-type retrotransposons RIRE2 and RIRE3 identified in rice previously. Interestingly, various portions of the RIRE7 sequence were homologous to several DNA segments present in the centromere regions of cereal chromosomes. Further cloning and nucleotide sequencing of fragments flanking RIRE7 copies showed that RIRE7 was inserted into a site within a tandem repeat sequence that has a unit length of 155 bp. The tandem repeat sequence, named TrsD, was homologous to tandem repeat sequences RCS2 and CentC, previously identified in the centromeric regions of rice and maize chromosomes. Fluorescence in situ hybridization (FISH) analysis of the metaphase chromosomes of O. sativa cv. Nipponbare showed that both RIRE7 and TrsD sequences were present in the centromere regions of the chromosomes. The presence of RIRE7 and the TrsD sequences in the centromere regions of several chromosomes was confirmed by the identification of several YAC clones whose chromosomal locations are known. Further FISH analysis of rice pachytene chromosomes showed that the TrsD sequences were located in a pericentromeric heterochromatin region. These findings strongly suggest that RIRE7 and TrsD are components of the pericentromeric heterochromatin of rice chromosomes.2001年, Molecular and General Genetics, 265(3) (3), 480 - 488, 英語[査読有り]研究論文（学術雑誌）
- Chromosome painting and genomic in situ hybridization (GISH) are both effective methods for basic genetic research and practical breeding. These methods were applied even in the typically small chromosomes of rice. This manuscript describes in detail, highly reproducible, complete protocols for chromosome painting and GISH in rice chromosomes. Examples of useful applications of these methods are also presented.2001年, Methods in Cell Science, 23(1-3) (1-3), 125 - 132, 英語研究論文（学術雑誌）
- High-resolution fluorescence in situ hybridization (FISH) on interphase and pachytene nuclei, and extended DNA fibers enabled microscopic distinction of DNA sequences less than a few thousands of base pairs apart. We applied this technique to reveal the molecular organization of telomere ends in japonica rice (Oryza sativa ssp. japonica), which consist of the Arabidopsis type TTTAGGG heptameric repeats and the rice specific subtelomeric tandem repeat sequence A (TrsA). Southern hybridizations of DNA digested with Bal31 and EcoRI, and FISH on chromosomes and extended DNA fibers demonstrated that (1) all chromosome ends possess the telomere tandem repeat measuring 3-4 kb (2) the subtelomeric TrsA occurs only at the ends of the long arms of chromosomes 6 and 12, and measure 6 and 10 kb, which corresponds to 231 and 682 copies for these sites, respectively (3) the telomere and TrsA repeats are separated by at most a few thousands of intervening nucleotide sequences. The molecular organization for a general telomere organization in plant chromosomes is discussed.2001年, Plant Molecular Biology, 47(3) (3), 413 - 421, 英語[査読有り]研究論文（学術雑誌）
- Genus Fragaria consists of several species, ranging from diploid to octoploid, and including various types of sex determination. The genome size of Fragaria species is not known correctly, mainly because preparation of samples suitable for genome size estimation has been difficult. Moreover, it is difficult to choose a suitable standard material for estimation given the small genome size of Fragaria species. Our experimental data showed that the flower petal is a more suitable tissue for flow cytometry. We developed a new procedure, which prevents contamination of tissue samples with debris, and thus allows more accurate estimation of the DNA content during flow cytometry. Arabidopsis thaliana has been sequenced nearly complete, and the estimated genome size from the sequence data is 125 Mbp. We chose Arabidopsis thaliana as standard material. Using this approach, we successfully estimated the genome size of the diploid Fragaria species to be around 164 Mbp/C.Japan Mendel Society, 2001年, Cytologia, 66(4) (4), 431 - 436, 英語[査読有り]研究論文（学術雑誌）
- Site-specific accumulation of a LINE-like retrotransposon in a sex chromosome of the dioecious plant Cannabis sativaMale-associated DNA sequences were analysed in hemp (Cannabis sativa L.), a dioecious plant with heteromorphic sex chromosomes. A male-associated DNA sequence in C. sativa (MADC1) and its flanking sequence encoded a reverse transcriptase that was strongly homologous to those of LINE-like retrotransposons from various plants and other organisms, as well as another open reading frame (ORF). Fluorescence in situ hybridization (FISH) with MADC1 as probe, which yielded strong signals specific for male genomic DNA in gel blot analysis, generated a clear doublet signal at the end of the long arm of the Y chromosome. FISH using pachytene chromosomes of pollen mother cells at meiotic prophase I revealed that pairing of X and Y chromosomes occurred at the short arm of the Y chromosome where MADC1 was not present. Furthermore, FISH using extended DNA fibers, with MADC1 and its flanking DNA as probes, revealed that 100 to 200 copies of the retrotransposon were located in tandem on the Y chromosome. These results support the hypothesis that accumulation of a specific LINE-like retrotransposon at the terminal region of the long arm of the Y chromosome might be one cause of heteromorphism of sex chromosomes.KLUWER ACADEMIC PUBL, 2000年12月, PLANT MOLECULAR BIOLOGY, 44(6) (6), 723 - 732, 英語[査読有り]研究論文（学術雑誌）
- Quantitative chromosome maps and rDNA localization in the T subgenome of Nicotiana tabacum L. and its putative progenitorsUsing DAPI-stained prometaphase chromosomes, quantitative idiograms were constructed for the T subgenome of Nicotiana tabacum (2n = 4x = 48, SSTT) and two putative candidates for its T subgenome progenitor, Nicotiana otophora and Nicotiana tomentosiformis (both have 2n = 24, TT). The large chromosomes of the three karyotypes could be identified from the distributional pattern of the DAPI signal. Fluorescence in situ hybridization (FISH) with 5S rDNA gave not only good cytogenetical landmarks for identification of small chromosomes of the karyotypes but also phylogenetical information. in all three idiograms, 5S rDNA was localized in the proximal region of the long arm of a small submetacentric pair, but an additional 5S rDNA locus was detected terminally on the short arm of a small metacentric pair in N. otophora. The 18S rDNA locus detected here corresponded to satellite regions in all three karyotypes. Two satellited pairs in N. otophora and one satellited pair in N. tomentosiformis had single large subterminal DAPI blocks and two interstitial DAPI bands on their long arms, respectively. For the T subgenome component of N. tabacum, the single intense DAPI band was depicted on the center of the long arm of a satellited pair in the idiogram, although two interstitial bands were often detected on the long arm of the satellited pair in some spreads. Therefore, it was suggested that the T component of N. tabacum was more similar to that of N. tomentosiformis than N. otophora, especially in respect of the number and location of rDNA and the distributional patterns of DAPI signals.SPRINGER-VERLAG, 2000年12月, THEORETICAL AND APPLIED GENETICS, 101(8) (8), 1180 - 1188, 英語[査読有り]研究論文（学術雑誌）
- 2000年11月, Jpn. Agr. Res.Quart., 英語Visual detection of useful genes on plant chromosomes.
- Quantification of total genomic DNA and selected repetitive sequences reveals concurrent changes in different DNA families in indica and japonica riceThis paper describes a fluorescence in situ hybridization (FISH) analysis of three different repetitive sequence families, which were mapped to mitotic metaphase chromosomes and extended DNA fibers (EDFs) of the two subspecies of rice (Oryza sativa), indica and japonica (2n = 2x = 24). The repeat families studied were(1) the tandem repeat sequence A (TrsA), a functionally non-significant repeat, (2) the [TTTA-GCG], telomere sequencer a non-transcribed, tandemly repeated but functionally significant repeat: and (3) the SS ribosomal RNA (5S rDNA). FISH of the TrsA repeat to metaphase chromosomes of indica and japonica cultivars revealed clear signals at the distal ends of twelve and four chromosomes, respectively. As shown ill a previous report, the 17S ribosomal RNA genes (17S rDNA) are located at the nucleolus organizers (NORs) on chromosomes 9 and 10 of the indica cultivar. However, the japonica rice lacked the rDNA signals on chromosome 10. The size of the 5S rDNA repeat block, which was mapped on the chromosome 11 of both cultivars, was 1.22 times larger in the indica than in the japonica genome. The telomeric repeat arrays at the distal ends of all chromosome arms were on average three times longer in the indica genome than in the japonica genome. Flow cytometric measurements revealed that the nuclear DNA content of indica rice is 9.7% higher than that of japonica rice. Our data suggest that different repetitive sequence families contribute significantly to the variation in genome size between indica and japonica rice, though to different extents. The increase or decrease in the copy number of several repetitive sequences examined here may indicate the existence of a directed change in genome size in rice. Possible reasons for this phenomenon of concurrent evolution of various repeat families are discussed.SPRINGER, 2000年04月, MOLECULAR AND GENERAL GENETICS, 263(3) (3), 388 - 394, 英語[査読有り]研究論文（学術雑誌）
- 2000年03月, Cytologia, 65, 325p-331p, 英語Quantitative chromosome map of Arabidopsis thaliana L. by imaging methods[査読有り]研究論文（学術雑誌）
- BIRKHAUSER VERLAG AG, 2000年, CHROMOSOMES TODAY, VOL 13, 13, 287 - +, 英語Smallness: gain and loss in plant chromosome research[査読有り]研究論文（国際会議プロシーディングス）
- Characterization of spinach chromosomes by condensation patterns and physical mapping of 5S and 45S rDNAs by FISHMolecular cytogenetic techniques and computer-aided karyotyping were applied to characterize the chromosomes of spinach (Spinacia oleracea L,, 2n = 12). Chromosome lengths, arm ratios, and degrees of condensation at prometaphase chromosomes were analyzed using a software Chromosome Image Analyzing System III (CHIAS III), DNA probes prepared from rice (Oryza sativa L.) rDNA were applied to the spinach chromosomes by the fluorescence in situ hybridization (FISH) method. Three 45S rDNA loci were detected at the nucleolar organizing region (NOR) of Chromosome 5, and at terminal positions of short arms of Chromosomes 2 and 6, The loci of 5S rDNA were also found at three locations. One was at the subtelomeric region of the long arm of Chromosome 2 and the other two were at the proximal region of the long arm of Chromosome 5, All spinach chromosomes were identified which will provide valuable information for mapping genes on these chromosomes.AMER SOC HORTICULTURAL SCIENCE, 2000年01月, JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 125(1) (1), 59 - 62, 英語[査読有り]研究論文（学術雑誌）
- KLUWER ACADEMIC/PLENUM PUBL, 2000年, GENOMES, , 109p-121p, 109 - 121, 英語Rice genome research: An alternative approach based on molecular cytology[査読有り]研究論文（国際会議プロシーディングス）
- Bryophyte 5S rDNA was inserted into 45S rDNA repeat units after the divergence from higher land plantsThe 5S ribosomal RNA genes (5S rDNA) are located independently from the 45S rDNA repeats containing 18S, 5.8S and 26S ribosomal RNA genes in higher eukaryotes. Southern blot and fluorescence in situ hybridization analyses demonstrated that the 5S rDNAs are encoded in the 45S rDNA repeat unit of a liverwort, Marchantia polymorpha, in contrast to higher plants. Sequencing analyses revealed that a single-repeat unit of the M. polymorpha nuclear rDNA, which is 16 103 bp in length, contained a 5S rDNA downstream of 18S, 5.8S and 26S rDNA. To our knowledge, this is the first report on co-localization of the 5S and 45S rDNAs in the rDNA repeat of land plants. Furthermore, we detected a 5S rDNA in the rDNA repeat of a moss, Funaria hygrometrica, by a homology search in a database. These findings suggest that there has been structural re-organization of the rDNAs after divergence of the bryophytes from the other plant species in the course of evolution.KLUWER ACADEMIC PUBL, 1999年11月, PLANT MOLECULAR BIOLOGY, 41(5) (5), 679 - 685, 英語[査読有り]研究論文（学術雑誌）
- Quantitative chromosome map of the polyploid Saccharum spontaneum by multicolor fluorescence in situ hybridization and imaging methodsSomatic chromosomes of a wild relative of sugarcane (Saccharum spontaneum L.) anther culture-derived clone (AP 85-361, 2n=32) were identified and characterized by computer-aided imaging technology and molecular cytological methods. The presence of four satellite chromosomes and four nearly identical chromosome sets suggests that the clone is a tetrahaploid with the basic number x=8. A quantitative chromosome map, or idiogram, was developed using image analysis of the condensation pattern (CP) at the prometaphase stage of somatic chromosomes. The 45S and 5S ribosomal RNA gene (rDNA) loci were simultaneously visualized by multi-color fluorescence in situ hybridization (McFISH) and precisely localized to the regions of 3p3.1 and 6q1.3 on the idiogram. The simultaneous visualization of two sets of four ribosomal RNA genes confirms tetraploidy of this clone. This conclusion is consistent with results of molecular marker mapping. The quantitative chromosome map produced will become the foundation for genome analyses based on chromosome identity and structure. Previously impossible identification of small chromosomes and untestable hypotheses about the polyploid nature of plants can now be settled with these two approaches of quantitative karyotyping and FISH.KLUWER ACADEMIC PUBL, 1999年04月, PLANT MOLECULAR BIOLOGY, 39(6) (6), 1165 - 1173, 英語[査読有り]研究論文（学術雑誌）
- 1999年02月, American Biotechnology Laboratory, 17(3) (3), 56 - 58, 英語Recent advances in the physical mapping of genes by fluorescence in situ hybridization (FISH) of rice研究論文（学術雑誌）
- Springer Netherlands, 1999年, Plant Molecular Biology, 41(2) (2), 293, 英語研究論文（学術雑誌）
- 18S ribosomal RNA gene (rDNA) loci were mapped on chromosomes of Paris tetraphylla A. Gray, P. verticillata M. v Bieberstein and P. polyphylla Smith, Liliaceae by using fluorescence in situ hybridization (FISH). The 18S rDNA probes were prepared from the interphase nuclei by the method during the polymerase chain reaction (PCR). Chromosome numbers of all the examined plants were 2n=10. Haploid genomes of P. tetraphylla and P. verticillata were consisted of three metacentric chromosomes, a submetacentric chromosomes and an acrocentric satellite chromosome. One 18S rDNA locus was detected at the second constriction of the acrocentric chromosome. On the other hand, a haploid genome of P. polyphylla was consisted of three metacentric chromosomes and two acrocentric chromosomes. Two 18S rDNA loci were detected at the second constriction of both acrocentric chromosomes.Japan Mendel Society, 1999年, Cytologia, 64(2) (2), 175 - 180, 英語[査読有り]研究論文（学術雑誌）
- Physical mapping of unique nucleotide sequences on identified rice chromosomesA physical mapping method for unique nucleotide sequences on specific chromosomal regions was developed combining objective chromosome identification and highly sensitive fluorescence in situ hybridisation (FISH). Four unique nucleotide sequences cloned from rice genomic DNAs, varying in size from 1.3 to 400 kb, were mapped on a rice chromosome map. A yeast artificial chromosome (YAC) clone with a 399 kb insert of rice genomic DNA was localised at the distal end of the long arm of rice chromosome (1q2.1) and a bacterial artificial chromosome (BAC) clone (180 kb) containing the rice leaf blast-resistant gene (Pi-b) was shown to occur at the distal end of the long arm of chromosome 2 (2q2.1). A cosmid (35 kb) with the resistance gene (Xa-21) against bacterial leaf blight was mapped on the interstitial region of the long arm on chromosome 11 (11q1.3). Furthermore a single RFLP marker, 1.29 kb in size, was mapped successfully to the distal region of the long arm of rice chromosome 3 (4q2.1). For precise localisation of the nucleotide sequences within the chromosome region, image analyses were effective. The BAC clone was localised to the specific region, 2q2.1:96.16, by image analysis. The result was compared with the known location of the BAC clone on the genetic map and the consistency was confirmed. The effectiveness and reliability in physically mapping nucleotide sequences on small plant chromosomes achieved by the FISH method using a variety of probes was unequivocally demonstrated.KLUWER ACADEMIC PUBL, 1998年12月, PLANT MOLECULAR BIOLOGY, 38(6) (6), 1043 - 1052, 英語[査読有り]研究論文（学術雑誌）
- 1998年12月, Rice Genetics News, 15, 83p-84p, 英語Characterization of parental genomes in a hybrid between Oryza sativa L. and O. officinalis Wall ex Watt. through fluorescence in situ hybridization.研究論文（学術雑誌）
- Isolation and FISH mapping of Yeast Artificial Chromosomes (YACs) encompassing an allele of the Gm2 gene for gall midge resistance in riceTen yeast artificial chromosomes (YACs) spanning the Gm2 locus have been isolated by screening high-density filters containing a total of approximately 7000 YAC (representing six genome equivalents) clones derived from a japonica rice, Nipponbare. The screening was done with five RFLP markers flanking a gall midge resistance gene, Gm2, which was previously mapped onto chromosome 4 of rice. This gene confers resistance to biotype 1 and 2 of gall midge (Orseolia oryzae), a major insect pest of rice in South and Southeast Asia. The RFLP markers RG214, RG329 and F8 hybridized with YAC Y2165. Two overlapping YAC clones (Y5212 and Y2165) were identified by Southern hybridization, with Gm2-flanking RFLP markers, and their inserts isolated. The purified YACs and RFLP markers flanking Gm2 were labeled and physically mapped by the fluorescence in situ hybridization (FISH) technique. All of them mapped to the long arm of chromosome 4 of the resistant variety of rice, 'Phalguna', confirming the previous RFLP mapping data.SPRINGER VERLAG, 1998年09月, THEORETICAL AND APPLIED GENETICS, 97(4) (4), 507 - 514, 英語[査読有り]研究論文（学術雑誌）
- RFLP analysis and genomic in situ hybridization (GISH) in somatic hybrids and their progeny between Lycopersicon esculentum and Solanum lycopersicoidesRFLP (restriction fragment length polymorphism) and GISH (genomic in situ hybridization) analyses were employed to identify the chloroplast and nuclear genomes of the somatic hybrids and progeny between tomato 'Ohgata zuiko' and Solanum lycopersicoides ('LA2386'). A random distribution of the chloroplast genotype was determined using a cloned 19.6-kb BamHI fragment (Bal) of tobacco chloroplast DNA. Eight selected hybrids were analyzed for their chromosomal compositions; 4 were tetraploids (2n = 48) with an equal number of chromosomes derived from each parent as accurately determined by GISH, and the other 4 were hexaploids, containing an average of two sets of tomato chromosomes and one set from the wild parent. RFLP analysis with six tomato nuclear probes of known chromosomal locations revealed no major variation among the 44 hybrid plants surveyed. However, it also showed the presence of both parent-specific alleles and the loss of some and the presence of a few non-parental alleles, indicating rearrangement and/or recombination of the nuclear DNA. The relevance of the molecular and cytological methods and the potential use of somatic hybrids for plant breeding are demonstrated.SPRINGER VERLAG, 1998年05月, THEORETICAL AND APPLIED GENETICS, 96(6-7) (6-7), 719 - 726, 英語[査読有り]研究論文（学術雑誌）
- Fesutuca-Lolium complexにおけるイタリアンライグラス(Lolium multiflorum Lam.)とトールフェスク4倍体亜種(Festuca arundinacea var. glaucescens Boiss.)のF_1雑種の有用性を明らかにするため, その農業特性と細胞遺伝学的特性調査を行った。胚培養法で作出したF_1雑種は形態的にはイタリアンライグラスに似ていた。農業特性(草丈, 出穂期, 再生草勢など)は両親の中間かイタリアンライグラスに近い値を示した。年間生草収量は4倍体イタリアンライグラス(品種, アキアオバ)や6倍体トールフェスク(品種, Lubrette, ホクリョウ)とほぼ同等であった。乾物分解率は6倍体トールフェスクと同等かやや優れていた。F_1雑種の花粉稔性は高く,種子生産量も多かった。これらの結果から, 本報で得られた4倍体属間雑種から新しい農業用フェストロリューム系統を作出する可能性が示された。細胞学的観察を行ったF_1雑種の中に異数体は含まれなかったが, 減数分裂期に多価染色体やI価染色体が確認された。また, Genomic in situ hybridization (GISH)法を用いることによりF_1雑種において両親に由来する染色体を識別することが可能であった。この染色体識別により後代での両親ゲノムの変異過程の詳細な解析が可能となった。日本草地学会, 1998年04月, Grassland Science, 44-1, 14p-21p(1) (1), 14 - 21, 英語[査読有り]研究論文（学術雑誌）
- Quantitative karyotyping of three diploid Brassica species by imaging methods and localization of 45s rDNA loci on the identified chromosomesChromosomes of the three diploid Brassica species, B. rapa (AA), B. nigra (BE) and B. oleracea (CC), were identified based on their morphological characteristics, especially on the condensation pattern appearing at the somatic pro-metaphase stage. The morphological features of the pro-metaphase chromosomes of the three Brassica spp. were quantified by imaging methods using chromosome image analyzing system II (CHIAS 2). As a result, quantitative chromosome maps or idiograms of the three diploid Brassica spp. were developed. The fluorescence in situ hybridization (FISH) method revealed the location of 45s rDNA (the 26s-5.8s-18s ribosomal RNA gene cluster) on the chromosomes involved. The number of 45s rDNA loci in the B. rapa, B. nigra and B. oleracea are five, three and two, respectively. The loci detected were systematically mapped on the idiograms of the three Brassica spp.SPRINGER VERLAG, 1998年03月, THEORETICAL AND APPLIED GENETICS, 96(3-4) (3-4), 325 - 330, 英語[査読有り]研究論文（学術雑誌）
- 1998年, The 12th Symposium on Plant Biotech, 12, 61p-70p, 英語Multi-dimensional analyses of plant chromosomes and genomes.研究論文（国際会議プロシーディングス）
- A multi-color genomic in situ hybridization (McGISH) method has been developed. Three different rice genomes, A, B and C, involved in rice somatic hybrids were distinguished using three different fluorescent signals. All the rice chromosomes from the differnet genomes could be identified by different fluorescent colors, and the distribution of each genome in the nucleus was clearly visualized under a fluorescence microscope. The relationship between chromosomal constitution and morphological variations observed in the somatic hybrids, and the utility of McGISH, are discussed based on the results currently obtained.1998年, Theoretical and Applied Genetics, 97(7) (7), 1013 - 1018, 英語[査読有り]研究論文（学術雑誌）
- Visual verification of close disposition between a rice A genome-specific DNA sequence (TrsA) and the telomere sequenceA rice A genome-specific tandem repeat sequence (TrsA) and telomeric nucleotide sequences, (TTTAGGG)(n), were simultaneously detected by multicolor fluorescence in situ hybridization (McFISH) using rice prometaphase chromosomes. Six pairs of TrsA sites visualized by fluorescence signals were all localized on the long arms close to the telomeric regions. Differences in the copy number of TrsA at the different sites were visualized both by the size of the telomeric condensation block stained with Giemsa solution and the signal intensity after FISH with TrsA. McFISH analyses using interphase nuclei could resolve close disposition of TrsA and telomere and also gave rough estimation of the distance between them. The functional significance of the close disposition of TrsA and telomere is discussed.SPRINGER, 1997年12月, PLANT MOLECULAR BIOLOGY, 35(6) (6), 963 - 968, 英語[査読有り]研究論文（学術雑誌）
- Repetitive sequences: cause for variation in genome size and chromosome morphology in the genus OryzaLarge variation in genome size as determined by the nuclear DNA content and the mitotic chromosome size among diploid rice species is revealed using flow cytometry and image analyses. Both the total chromosomal length (r = 0.939) and the total chromosomal area (r = 0.927) correlated well with the nuclear DNA content. Among all the species examined, Oryza australiensis (E genome) and O. brachyantha (F genome), respectively, were the largest and smallest in genome size. O. sativa (A genome) involving all the cultivated species showed the intermediate genome size between them. The distribution patterns of genome-specific repetitive DNA sequences were physically determined using fluorescence in situ hybridization (FISH). O. brachyantha had limited sites of the repetitive DNA sequences specific to the F genome. O. australiensis showed overall amplification of genome-specific DNA sequences throughout the chromosomes. The amplification of the repetitive DNA sequences causes the variation in the chromosome morphology and thus the genome size among diploid species in the genus Oryza.KLUWER ACADEMIC PUBL, 1997年12月, PLANT MOLECULAR BIOLOGY, 35(6) (6), 791 - 799, 英語[査読有り]研究論文（学術雑誌）
- Chromosomal location and reorganization of the 45S and 5S rDNA in the Brachyscome lineariloba complex (Asteraceae)The chromosomal locations of the 45S (18S-5.8S-26S) and 5S ribosomal DNA in the Brachyscome lineariloba complex and two related species have been determined by the use of multicolor fluorescence in situ hybridization (McFISH). The Brachyscome lineariloba complex includes five cytodemes with 2n=4,8,10,12 and 16, Each of the 5S and 45S rDNA loci occurs at two sites on chromosomes in cytodemes with 2n=4, While in cytodemes with 2n=8, 10, 12 and 16, the number of 5S rDNA sites increases from four to eight paralleled to the genomic addition of diploid (4 chromosomes) or haploid (2 chromosomes) dosage, Of the 5S rDNA sites, only one pair is major, except for the cytodeme with 2n=10. The remaining 5S rDNA sites are minor and seem to have reduced the unit number of the 5S rDNA during the successive genomic additions, The 45S rDNA site is detected only at two nucleolar organizing regions in all cytodemes regardless of successive genomic addition, The loss or diminution of 45S rDNA sequences seem to have proceeded more rapidly than 5S rDNA sequences in the B. lineariloba complex.BOTANICAL SOC JAPAN, 1997年09月, JOURNAL OF PLANT RESEARCH, 110(1099) (1099), 371 - 377, 英語[査読有り]研究論文（学術雑誌）
- 1997年06月, Chinese Journal of Genetics, 24-3, 199p-223p, 英語Analysis of the Chromosome Composition of Common Wheat-Agropyron intermedium Partial Amphiploid, Yuan Zhong 2 by in situ hybridization.[査読有り]研究論文（学術雑誌）
- Construction of an 800-kb contig in the near-centromeric region of the rice blast resistance gene Pi-ta(2) using a highly representative rice BAC libraryWe constructed a rice Bacterial Artificial Chromosome (BAG) library from green leaf protoplasts of the cultivar Shimokita harboring the rice blast resistance gene Pi-ta. The average insert size of 155 kb and the library size of seven genome equivalents make it one of the most comprehensive BAC libraries available, and larger than many plant YAC libraries. The library clones were plated on seven high density membranes of microplate size, enabling efficient colony identification in colony hybridization experiments. Seven percent of clones carried chloroplast DNA. By probing with markers close to the blast resistance genes Pi-ta(2) (closely linked to Pi-ta) and Pi-b, respectively located in the centromeric region of chromosome 12 and near the telomeric end of chromosome 2, on average 2.2 +/- 1.3 and 8.0 +/- 2.6 BAC clones/marker were isolated. Differences in chromosomal structures may contribute to this wide variation in yield. A contig of about 800 kb, consisting of 19 clones, was constructed in the Pi-ta(2) region. This region had a high frequency of repetitive sequences. To circumvent this difficulty, we devised a ''two-step walking'' method. The contig spanned a 300 kb region between markers located at 0 cM and 0.3 cM from Pi-ta(2). The ratio of physical to genetic distances (> 1,000 kb/cM) was more than three times larger than the average of rice (300 kb/cM). The low recombination rate and high frequency of repetitive sequences may also be related to the near centromeric character of this region. Fluorescent in situ hybridization (FISH) with a BAC clone from the Pi-b region yielded very clear signals on the long arm of chromosome 2, while a clone from the Pi-ta(2) region showed various cross-hybridizing signals near the centromeric regions of all chromosomes.SPRINGER VERLAG, 1997年05月, MOLECULAR & GENERAL GENETICS, 254(6) (6), 611 - 620, 英語[査読有り]研究論文（学術雑誌）
- Identification of Parental Chromosomes in the Interspecific Hybrids of Nicotiana rustica L. × N. tabacum L. and N. gossei Domin × N. tabacum L., Using Genomic in situ HybridizationGenomic in situ hybridization was carried out for mitotic cells of the interspecific hybrids of Nicotiana rustica L, (2 n = 48) x N, tabacum L, (2 n = 48) and N, gossei Domin (2 n = 36) x N, tabacum L, Without blocking DNA of N, tabacum, clear hybridization signals from the biotinylated total genomic DNA of N, rustica or N, gossei, were obtained for the set of chromosomes, indicating that each parent could be discriminated, In the hybrid of N, rustica x N, tabacum, twenty-four chromosomes among forty-eight chromosomes were confirmed to be derived from N, rustica, Furthermore, two chromosomes exhibited regions with weak hybridization signals, suggesting that interspecific translocations might have occurred between N, rustica and N, tabacum chromosomes, On the other hand, in the hybrid of N, gossei x N, tabacum, eighteen chromosomes showing a hybridization signal over the entire length, were discriminated from the forty-two hybrid chromosomes. No region with weak signal was detected in any of them.JAPANESE SOC BREEDING, 1997年03月, Breeding Science, 47(1) (1), 67 - 70, 英語[査読有り]研究論文（学術雑誌）
- Genomic in situ hybridization was carried out for mitotic cells of the interspecific hybrids of Nicotiana rustica L. (2 n = 48) x N. tabacum L. (2 n = 48) and N. gossei Domin (2 n = 36) x N. tabacum L. Without blocking DNA of N. tabacum, clear hybridization signals from the biotinylated total genomic DNA of N. rustica or N. gossei were obtained for the set of chromosomes, indicating that each parent could be discriminated. In the hybrid of N. rustica x N. tabacum, twenty-four chromosomes among forty-eight chromosomes were confirmed to be derived from N. rustica. Furthermore, two chromosomes exhibited regions with weak hybridization signals, suggesting that interspecific translocations might have occurred between N. rustica and N. tabacum chromosomes. On the other hand, in the hybrid of N. gossei x N. tabacum, eighteen chromosomes showing a hybridization signal over the entire length, were discriminated from the forty-two hybrid chromosomes. No region with weak signal was detected in any of them.Japanese Society of Breeding, 1997年, Breeding Science, 47(1) (1), 67 - 70, 英語[査読有り]研究論文（学術雑誌）
- 1996年12月, Maize Genet. Coop. Newslett, 71, 162p-166p, 英語The distribution of 5-methylcytosine in somatic chromosomes of germinating aged rice seeds研究論文（学術雑誌）
- 1996年03月, Maize Genet. Coop. Newslett, 70, 60p-61p, 英語Heterochromatic knob-specific repeated sequence is associated with the formation of chromosome bridges in cultured cells and in germinating roots of aged seeds.研究論文（学術雑誌）
- 1995年06月, Theor. Appl. Genet., 91, 212 - 217, 英語Cytological studies of African cultivated rice, Oryza glaberrima Steud. Theor. Appl.研究論文（学術雑誌）
- The 17 s-5.8 s-25 s ribosomal RNA gene (rDNA) loci in Oryza spp. were identified by the fluorescence in-situ hybridization (FISH) method. The rDNA loci were located on one-to-three chromosomes (two-to-six sites) within the eight diploid Oryza spp. One of the rDNA loci gave the weakest hybridization signal. This locus is reported for the first time in the genus Oryza. The chromosomes containing the rDNA loci were determined to be numbers 9, 10 and 11 in descending order of the copy number of rDNA. The application of image analysis methods, after slide preparation treatments (post-treatments), and the use of a thermal cycler, greatly improved the reproducibility of the results. The evolutionary significance of the variability of rDNA loci among the Oryza spp. is discussed. © 1994, Springer-Verlag. All rights reserved.1994年, Theoretical and Applied Genetics: International Journal of Plant Breeding Research, 87(8) (8), 893 - 899, 英語研究論文（学術雑誌）
- MICRODISSECTION OF PLANT CHROMOSOMES BY ARGON-ION LASER-BEAMRice and barley chromosomal samples were prepared both on a polyester membrane and on an ordinary glass slide and subjected to microdissection by an argon-ion laser. The intensity and the position of the laser beam were controlled by a microcomputer. The most suitable intensity to obtain chromosomal fragments was determined experimentally. As a result, specific regions of the centromere, satellite, short arm, or long arm, of the barley and rice chromosomes were dissected out from the chromosomal spreads. Chromosomal fragments were also successfully transferred from the sample into an Eppendorf tube.SPRINGER VERLAG, 1992年09月, THEORETICAL AND APPLIED GENETICS, 84(7-8) (7-8), 787 - 791, 英語[査読有り]研究論文（学術雑誌）
- 2023年05月, 日本科学教育学会研究会研究報告, 37(6) (6), 59 - 62, 日本語
- 2023年02月, 日本科学教育学会研究会研究報告, 37(5) (5), 75 - 78, 日本語
- 2018年, 園芸学研究 別冊, 17(1) (1)ホウレンソウの雄決定遺伝子座周辺から見出された組換抑制領域の分子構造
- 日本生物工学会, 2011年, 日本生物工学会大会講演要旨集, 63, 197 - 197, 英語2La12 Phylogenetic analysis of Ty1-copia retrotransposons in Jatropha curcas L. :
- 日本理科教育学会近畿支部大会実行委員会, 2011年, 日本理科教育学会近畿支部大会(大阪大会)発表要旨集, 2011, 61 - 61, 日本語P15 アーギュメンテーション・スキルの育成を目指した実践的研究 : 小学校第6学年の理科授業デザイン(研究発表(ポスター発表))
- Recent advances in rice genome and chromosome structure research by fluorescence in situ hybridization (FISH).Fluorescence in situ hybridization (FISH) is an effective method for the physical mapping of genes and repetitive DNA sequences on chromosomes. Physical mapping of unique nucleotide sequences on specific rice chromosome regions was performed using a combination of chromosome identification and highly sensitive FISH. Increases in the detection sensitivity of smaller DNA sequences and improvements in spatial resolution have ushered in a new phase in FISH technology. Thus, it is now possible to perform in situ hybridization on somatic chromosomes, pachytene chromosomes, and even on extended DNA fibers (EDFs). Pachytene-FISH allows the integration of genetic linkage maps and quantitative chromosome maps. Visualization methods using FISH can reveal the spatial organization of the centromere, heterochromatin/euchromatin, and the terminal structures of rice chromosomes. Furthermore, EDF-FISH and the DNA combing technique can resolve a spatial distance of 1 kb between adjacent DNA sequences, and the detection of even a 300-bp target is now feasible. The copy numbers of various repetitive sequences and the sizes of various DNA molecules were quantitatively measured using the molecular combing technique. This review describes the significance of these advances in molecular cytology in rice and discusses future applications in plant studies using visualization techniques.2010年, Proceedings of the Japan Academy. Series B, Physical and biological sciences, 86(2) (2), 103 - 16, 英語, 国内誌[査読有り]
- 羊土社, 2007年07月, バイオテクノロジージャーナル, 7(4) (4), 460 - 463, 日本語テクノ・トレンド FISH法の新しい展開とそれを用いた高精細ゲノム解析法
- Society of Chromosome Research, 2007年, Chromosome science, 10(4) (4), 124 - 124, 英語P-04. Establishment of red clover chromosome map by the improved chromosome image analyzing system, CHIAS4(Abstracts for the oral and the poster presentations,Abstracts of the Joint Meeting: the 58th Annual Meeting of the Society of Chromosome Research and the 17th Annual Meeting of the Chromosome Colloquium) :
- Society of Chromosome Research, 2007年, Chromosome science, 10(4) (4), 115 - 115, 英語O-16. The characteristic condensation patterns in rice chromosome(Abstracts for the oral and the poster presentations,Abstracts of the Joint Meeting: the 58th Annual Meeting of the Society of Chromosome Research and the 17th Annual Meeting of the Chromosome Colloquium) :
- Society of Chromosome Research, 2007年, Chromosome science, 10(4) (4), 115 - 115, 英語O-15. Construction of red clover (Trifolium pratense L.) chromosome map(Abstracts for the oral and the poster presentations,Abstracts of the Joint Meeting: the 58th Annual Meeting of the Society of Chromosome Research and the 17th Annual Meeting of the Chromosome Colloquium) :
- SPRINGER, 2007年, CHROMOSOME RESEARCH, 15, 56 - 56, 英語Legume chromosome studies of Trifolium pratense and Lotus japonicus研究発表ペーパー・要旨（国際会議）
- SPRINGER, 2007年, CHROMOSOME RESEARCH, 15, 55 - 56, 英語Quantitative and fine rice pachytene chromosome map by analyzed chromomeric features研究発表ペーパー・要旨（国際会議）
- Society of Chromosome Research, 2006年, Chromosome science, 9(4) (4), 127 - 127, 英語O-32. Integration of linkage and chromosome maps of red clover(Trifolium pratense L.)(Abstracts of the oral and poster presentations) :
- Rice chromosome research was initiated around 1910 and has been progressing ever since. Twelve pairs of chromosomes (2n = 24) and a 440 Mb genome, the smallest genome among all main cereal crops, have been identified. According to the recent rice chromosome researches, all chromosomes were well identified over the disadvantages of small chromosome size and quantitative rice chromosomal maps have been developed using chromosome image analysis system (CHIAS). Moreover, agriculturally important genes have been detected using new direct DNA visualization methods with fluorescence in situ hybridization (FISH). This review describes the recent progress in molecular and digital cytogenetics using visualization from a historical perspective, highlighting the future prospects in rice chromosome science.The Japan Academy, 2005年10月, The Proceedings of the Japan Academy, Series B., 12: 382-392.(9) (9), 382 - 392, 英語
(Communicated by Eishiro SHIKATA, M.J.A.)[査読有り]記事・総説・解説・論説等（学術雑誌） - Rice chromosome research was initiated around 1910 and has been progressing ever since. Twelve pairs of chromosomes (2n = 24) and a 440 Mb genome, the smallest genome among all main cereal crops, have been identified. According to the recent rice chromosome researches, all chromosomes were well identified over the disadvantages of small chromosome size and quantitative rice chromosomal maps have been developed using chromosome image analysis system (CHIAS). Moreover, agriculturally important genes have been detected using new direct DNA visualization methods with fluorescence in situ hybridization (FISH). This review describes the recent progress in molecular and digital cytogenetics using visualization from a historical perspective, highlighting the future prospects in rice chromosome science.The Japan Academy, 2005年04月, The Proceedings of the Japan Academy, Series B12., vol. 81, pp.382-392(9) (9), 382 - 392, 英語
(Communicated by Eishiro SHIKATA, M.J.A.)[査読有り]記事・総説・解説・論説等（学術雑誌） - 2005年, 日本分子生物学会年会講演要旨集, 28thアカクローバゲノムの構造解析
- 2005年, 大学教育出版, 日本語キーワード 人間と発達 V. 生活とテクノロジー記事・総説・解説・論説等（大学・研究所紀要）
- 共立出版, 2004年08月, 蛋白質核酸酵素, 49(11) (11), 1828 - 1833,1459, 日本語ゲノミクス解析システム ゲノム構造解析システム (バイオ高性能機器・新技術利用マニュアル) -- (生体高分子の包括的解析のためのシステム)
- 2004年08月, 蛋白質核酸酵素, No. 8 -11, pp. 1828-1833, 日本語ゲノム構造解析システム記事・総説・解説・論説等（学術雑誌）
- 日本理科教育学会近畿支部大会実行委員会, 2004年, 日本理科教育学会近畿支部大会(大阪大会)発表要旨集, 2004, 72 - 72, 日本語P-15 Knowledge Forumを利用した学習環境のデザイン実験 : 遺伝子組換え食品を題材にした単元の開発III
- 日本生物工学会, 2004年, 日本生物工学会大会講演要旨集, 16, 68 - 68, 日本語1pC16-2 間接蛍光抗体法を用いた染色体関連蛋白質の局在解析(ペプチド工学・プロテオーム,一般講演)
- 2004年, 日本分子生物学会年会プログラム・講演要旨集, 27thアカクローバ高密度連鎖地図の作製
- 日本育種学会, 2003年12月01日, 育種学研究, 5(4) (4), 183 - 191, 日本語超微細蛍光in situ hybridization法の開発とそれによる植物遺伝子のマッピング(特集記事)
- 2003年10月11日, 日本教育工学会大会講演論文集, 19(2) (2), 741 - 742, 日本語遺伝子組み換え食品問題に対する社会的意思決定をテーマとした科学教育のたあの CSCL 環境 : 学習者からみた有効性
- 2002年03月30日, 育種学研究 = Breeding research, 4(1) (1), 6 - 7, 日本語超微細蛍光in situ hybridization (FISH)法の開発とそれによるイネ遺伝子のマッピング
- 一般社団法人日本機械学会, 2002年03月01日, バイオエンジニアリング講演会講演論文集, 2002(14) (14), 223 - 224, 日本語2153 AFM を用いた DNA・蛋白質相互作用の可視化
- 2000年09月25日, 育種学研究 = Breeding research, 2(2) (2), 30 - 30, 日本語フローサイトメトリーを用いたイチゴゲノムサイズの解析
- 2000年09月25日, 育種学研究 = Breeding research, 2(2) (2), 2 - 2, 日本語画像解析によるイネ第9染色体パテキン期高精度定量的染色体地図の作成
- Visual detection of useful genes on plant chromosomesIn this manuscript the current status of direct visualization of gene location on chromosomes was reviewed by considering 5 aspects as follows: principle of in situ hybridization (ISH) method, historical perspective of mapping genes by in situ hybridization, improvement in the sensitivity of fluorescence in situ hybridization (FISH), visualization of location of useful genes on chromosomes, and prospects for visualization of useful genes.TROPICAL AGRICULTURE RES CENTR, 2000年07月, JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY, 34(3) (3), 153 - 158, 英語
- 2000年04月, 育種学研究 = Breeding research, 2(1) (1), 84 - 84, 日本語Rolling circle amplification (RCA)法によるDNA配列変異の高感度検出.
- 2000年03月26日, 園芸学会雑誌. 別冊, 園芸学会大会研究発表, 69(1) (1), 238 - 238, 日本語高再分化能ハクサイ'Homei'およびキャベツ'松波'小胞子由来再分化植物の染色体数
- Arabidopsis thaliana L. has 10 chromosomes that are small and similar in morphology. Molecular cytogenetics and image analyses were applied to characterize the somatic chromosomes. Prometaphase chromosomes were quantitatively analyzed based on their patterns of condensation, because they show a prominent uneven condensation along the chromosomes. The total length of the haploid prometaphase chromosomes is 16.5 μm, which is about 1.8 times the size of the metaphase chromosomes. We also detected the 45S (18S-5.8S-25S) and 5S rDNA loci by fluorescence in situ hybridization (FISH) and determined their locations on the chromosomes by the imaging methods. As a result, a quantitative chromosome map based on the condensation pattern was developed for the first time in Arabidopsis with the locations of rDNAs by using imaging methods. It is now possible to localize genes precisely on this map.Japan Mendel Society, International Society of Cytology, 2000年, Cytologia, 65(3) (3), 325 - 331, 英語
- Society of Chromosome Research, 2000年, Chromosome science, 4(4) (4), 132 - 132, 英語Development of M-FISH using custom labeled nucleotide synthesis. :
- 1999年03月, Plant and cell physiology, 40, s27 - s27, 英語MALE-SPECIFIC RAPD MARKERS IN Cannabis sativa CODED RETROTRANSPOSONS
- 1998年12月01日, 日本分子生物学会年会プログラム・講演要旨集, 21, 321 - 321, 日本語染色体情報の解析・利用に関する研究118. Fiber-FISH法によるゲノム内部構造の可視化解析
- 1998年09月, 育種学最近の進歩, 40, 55 - 58, 日本語分子細胞学的手法を用いた異種遺伝子・ゲノムの検出
- 1998年05月, Plant and cell physiology, 39, S115 - S115, 英語ESTIMATION OF COPY NUMBER BY HIGH RESOLUTION FISH USING EXTENDED DNA FIBERS IN RICE
- KARGER, 1998年, CYTOGENETICS AND CELL GENETICS, 81(2) (2), 144 - 144, 英語Paralleled changes of repetitive sequences in the Oryza genomes研究発表ペーパー・要旨（国際会議）
- KARGER, 1998年, CYTOGENETICS AND CELL GENETICS, 81(2) (2), 132 - 132, 英語Chromosome analysis of hybrids in the Japanese wild lily by genomic in situ hybridization研究発表ペーパー・要旨（国際会議）
- 日本育種学会, 1997年12月01日, Breeding science, 47(4) (4), 410 - 410, 日本語植物染色体情報の解析・利用に関する国際ワークショップ
- 1997年09月, 日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan, 61, 314 - 314, 日本語染色体情報の利用と解析に関する研究 105. DNAファイバー上でのFISH法およびその定量的解析
- 1997年09月, 日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan, 61, 292 - 292, 日本語Brachyscome lineariloba 複合体 (2n=4, 8, 10, 12, 16) における r-DNAの染色体上の分布
- 1996年, Rice Genet Newsl.., 13, 89 - 93A new manual for fluorescence in situ hybridization (FISH) in plant chromosomes
- 北海道大学, 1995年09月, 北海道大學農學部紀要, 66(3) (3), 277 - 320, 英語Rice chromosomes studies by fluorescence in situ hybridization with special reference to physical mapping and chromosome structure
- PHYSICAL MAPPING OF RICE DNAS BY AN IMPROVED FISH METHODAn improved fluorescence in situ hybridization (FISH) method for localizing genes and DNA sequences on rice chromosomes has been developed in our laboratory. Three improvements in the fluorescence in situ hybridization procedures, i.e. development of four steps of posttreatments, development of a modified thermal cycler and introduction of an imaging method enabled to obtain clear and reproducible signals on rice chromosomes. The improved FISH method was employed for physical mapping of 45S ribosomal RNA genes in Oryza species. Variability in the number of 45S rDNA loci was detected among eight diploid species. The FISH method was also applied to localize the rice repeated sequences. The repeated sequences were adjacent to the telomeric regions of the long arms of chromosomes. Furthermore, a multicolor FISH (McFISH) method was developed and was applied to characterize 0. glaberrima chromosomes.TROPICAL AGRICULTURE RES CENTR, 1995年04月, JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY, 29(2) (2), 83 - 88, 英語
- 1995年, Rice Biotech. Quart., 24, 20 - 20Cytological studies on African cultivated rice.
- 1994年, Molec. Gen. Genet., 245, 449 - 455Involvement of transposition in dispersion of tandem repeat sequences (TrsA) in rice genomes.
- 1993年, Rice Genome, 2, 14 - 15Reliable FISH method for rice chromosomes
- その他, Springer International Publishing AG, 2017年11月, 英語, Jatropha curcas is a monoecious species, which forms unisexual flowers. The unisexual flowers are produced on the same inflorescence with 10 to 30-folds higher ratio of male flowers to female flowers. The bias in the inflorescence limits Jatropha seed production. Flowering genes analysis would provide important knowledge about the multiple environmental and endogenous factors t, ISBN: 9783319496535学術書
- 共著, Springer, 2016年12月, 英語, Advances in cytology have led to the application of a wide range of visualization methods in plant genome studies. Image analysis methods are indispensable tools where morphology, density, and color play important roles in the biological systems. Development of visualization and image analysis methods are useful techniques in the analyses of the detailed structure and functionImage Analysis of DNA fiber and nucleus in plants.学術書
- 共著, CRC Press, 2014年06月, 英語Plant Image Analysis: Fundamentals and Applications.学術書
- 共著, 東京化学同人, 2010年, 日本語生物学辞典学術書
- 共著, ユニバーサルアカデミープレス, 2009年01月, 日本語植物ゲノム科学辞典学術書
- 共著, Taylor and Francis, 2009年01月, 英語Dynamic and functional analysis of chromosomal proteins.学術書
- 共著, Springer-Verlag Berlin Heidelberg, 2009年01月, 英語Chromosome dynamics in tobacco BY-2 cultured cells.学術書
- 共著, 大学教育出版, 2007年04月, 日本語キーワード 人間と発達［増補改訂版］ V. 生活とテクノロジー教科書・概説・概論
- 共著, Taylor and Francis, 2007年, 英語Dynamic and functional analysis of chromosomal proteins, in:"Chromosome Nanoscience and Technology (eds.) K. Fukui and T. Ushiki"学術書
- 共著, Springer, 2007年01月, 英語Chromosome dynamics in tobacco BY-2 cultured cells, in:"Biotechnology in agriculture and forestry, 58: 51-63. (Ed. T. Nagata, K. Matsuoka, D. Inze) Tabacco NY-2 cells: From Cellular Dynamics to Omics"学術書
- 共著, 養賢堂, 2006年04月, 日本語, 染色体の研究に関してクロモソーム植物染色体研究の方法教科書・概説・概論
- 共著, Cytologia, 2005年03月, 英語Comparision of Surface Structures between Extended and Condensed Stages of Barley Chromosomes Revealed with Atomic Force Microscopy学術書
- 共著, Genes Genet. Syst., 2005年02月, 英語Fluorescent labeling of plant chromosomes in suspension by FISH学術書
- 共著, Rice Genetics, 2004年12月, 英語Recent advances in FISH analysis of plant chromosomes学術書
- 共著, 蛋白質 核酸 酵素, 2004年08月, 日本語ゲノム構造解析システム一般書・啓蒙書
- 共著, Plant Biotechnology, 2004年07月, 英語Application of the Bio-Active Beads Method in Rice Transformation学術書
- 共著, Genetic diversity, 2004年, 英語High-resolution fluorescence in situ hybridization (FISH) for gene mapping and molecular analysis of rice chromosomes学術書
- 共著, IRRI, 2004年01月, 英語Recent advances in FISH analysis of plant chromosomes. In: "Advances in Rice Genetics"学術書
- 共著, Handbook for Plant Metabolic Engineering, 2002年04月, 英語遺伝子マッピング学術書
- 共著, 日本生物工学会, 2002年04月, 英語FISH解析法学術書
- 共著, Chromosomes Today, 2000年12月, 英語Smallness: gain and loss in plant chromosome research.学術書
- 共著, Japan Agricultural Research Quarterly (JARQ), 2000年06月, 英語Visual Detection of Useful Genes on Plant Chromosomes.学術書
- 単著, 化学と生物, 2000年06月, 日本語DNAの位置や遺伝子をカラーで見る一般書・啓蒙書
- 単著, 化学と生物, 2000年03月, 日本語マルチカラーFISH法によるヒト染色体の核型分析．一般書・啓蒙書
- 単著, Biosci. Biotech., 2000年, 英語Visualization of DNA sequences and gene by multi colorzation.学術書
- 共著, Genome science protocols in plants. Plant Cell Technology series 14, 2000年, 英語Advanced development of visualization techniques for plant genome.学術書
- 共著, American Biotechnology Laboratory, 1999年02月, 英語Recent advances in the physical mapping of genes by fluorescence in situ hybridization (FUISH) of rice.学術書
- 共著, BRAIN Techno News, 1999年, 英語FISH method visualizes DNA and genes on chromosomes and genomes.一般書・啓蒙書
- 共著, クロモソーム, 1998年03月, 日本語蛍光in situハイブリダイゼーション法を用いた分子細胞生物学学術書
- 共著, Recent advance in plant breeding, 1998年, 英語Analysis of transgene and genome by molecular cytological analysis学術書
- 共著, Rice Genetics III, 1997年, 英語Physical mapping of several genes in rice using fluorescent in situ hybridization.学術書
- Med school of Silesia syposium, 2024年12月, 英語Nano-visualization by advanced electron microscopy in chromosome research.[招待有り]公開講演，セミナー，チュートリアル，講習，講義等
- Silesia Univ symposium, 2024年12月, 英語Repeatome and Cytogenetics Landscapes in Ornamental Hortensias[招待有り]公開講演，セミナー，チュートリアル，講習，講義等
- UEB Symposium, 2024年12月, 英語Plant Chromosome Architecture: Nanoscale Observations with Cutting-Edge Microscopy..[招待有り]公開講演，セミナー，チュートリアル，講習，講義等
- 第75回染色体学会年会, 2024年10月, 日本語異質倍数性は野生植物の都市環境適応に有効にはたらく
- 第74回染色体学会年会, 2023年10月, 日本語アジサイ属の遺伝的多様性：6種のゲノムリピトームと染色体解析口頭発表（一般）
- The 8th Asia-Pacific Chromosome Colloquium (APCC8), 2023年09月, 英語Nano-Level Insights into Chromosome Biology: High-Resolution Imaging with Advanced Microscopy.[招待有り]口頭発表（招待・特別）
- Plant Chromosome Biology: Cytogenetics meeting 2023, 2023年09月, 英語Urban environments, chromosome formula and polyploidization in two Commelina speciesポスター発表
- The 8th Asia-Pacific Chromosome Colloquium (APCC8), 2023年09月, 英語Urban adaptation of Commelina communis related to polyploidization.ポスター発表
- TheAsia-Pacific Chromosome Colloquium (APCC8), 2023年09月, 英語Comparative clustering analysis reveals diversity of repetitive elements in the genus Hydrangea.ポスター発表
- The 8th Asia-Pacific Chromosome Colloquium (APCC8), 2023年08月, 英語Improved CRISPR imaging techniques for target-specific DNA sequences口頭発表（一般）
- Plant Chromosome Biology: Cytogenetics meeting 2023, 2023年09月, 英語Unveiling the Nano-World of Plant Chromosomes: Chromatin Fiber Compaction and Scaffold Protein Localization using Advanced Microscopy Techniques.口頭発表（一般）
- Plant Chromosome Biology: Cytogenetics meeting 2023, 2023年09月, 英語Major genotype-panels for Hydrangea genomics by comparative repeat analysis.ポスター発表
- 日本生態学会第70回大会, 2023年03月, 日本語在来一年生草本ツユクサの形質進化の検証 -多様な都市環境に着目して-ポスター発表
- 2022年度第5回日本科学教育学会研究会（九州・沖縄支部開催）, 2023年02月, 日本語科学技術の社会問題としてのゲノム編集を題材とした小学生向け教育プログラムの開発口頭発表（一般）
- 一般財団法人 染色体学会 第 73 回（2022 年度）年会, 2022年10月, 日本語全ゲノム解析によるアジサイの反復配列様式とサテライトDNAの解析口頭発表（一般）
- 一般財団法人 染色体学会 第 73 回（2022 年度）年会, 2022年10月, 英語Ty1-copia retrotransposons variation in Crinum thaianum (water-onion) endangered species in Thailand口頭発表（一般）
- 一般財団法人 染色体学会 第 73 回（2022 年度）年会, 2022年10月, 日本語超高圧電子顕微鏡によるトポイソメラーゼ局在と植物染色体高次構造口頭発表（一般）
- 第72回 一般財団法人 染色体学会年会, 2021年09月, 日本語植物染色体は動物細胞の中でどのように適応していくのか？口頭発表（一般）
- 第72回 一般財団法人 染色体学会年会, 2021年09月, 日本語Helium ion microscope reveals chromatin fiber compaction in barley chromosomeポスター発表
- The 23rd International Chromosome Conference, 2021年07月Helium ion microscope reveals chromatin fiber compaction in barley chromosomeポスター発表
- The 23rd International Chromosome Conference, 2021年07月, 英語How do plant chromosomes genetically behave and modify in human cells?口頭発表（一般）
- Asian Pacific Chromosome Colloquium 7, 2020年11月, 英語Outer structure of barley (Hordeum vulgare) chromosome using Helium Ion Microscopeポスター発表
- Asian Pacific Chromosome Colloquium 7, 2020年11月, 英語Genetics and Epigenetics of Plant Chromosomes in a Human-plant Hybrid Cell Lineポスター発表
- 5th Nano- Chromosome workshop, 2020年02月, 英語Complex genomic and chromosome rearrangement in Human-Arabidopsis hybrid口頭発表（一般）
- 5th Nano- Chromosome workshop, 2020年02月, 英語Isolation of Barley chromosome for tomography analysis[招待有り]口頭発表（一般）
- 5th Nano- Chromosome workshop, 2020年02月, 英語Insight of epigenetical chromatin dynamics in plant cell.[招待有り]口頭発表（一般）
- 財団法人染色体学会第70回年会, 2019年09月, 日本語環境ストレス条件下での植物細胞のエピジェネティク変動口頭発表（一般）
- 財団法人染色体学会第70回年会, 2019年09月, 英語Reconstruction of Arabidopsis chromosome in human-plant hybrid cellポスター発表
- 財団法人染色体学会第70回年会, 2019年09月, 英語Visualization of chromosome scaffold structure using electron tomography口頭発表（一般）
- The 1st International Conference on Agriculture and Rural Development (ICARD), 2019年08月, 英語Morphology changes of rice root nucleus under iron stress.口頭発表（一般）
- 21st National Genetics Conferences, 2019年06月, 英語Insights of cytogenetics research for rice genetics and breeding[招待有り]口頭発表（招待・特別）
- The 3rd international symposium. e-Asia meeting, 2019年06月, 英語Development of nano-visualization for structural analyses of chromosome[招待有り]口頭発表（一般）
- The 3rd international symposium. e-Asia meeting, 2019年06月, 英語Histone dynamics under environmental stress in plants[招待有り]口頭発表（招待・特別）
- 第66回日本生態学会大会, 2019年03月, 英語, 神戸国際会議場・展示場（神戸市）, 国内会議Visualizing environmental DNA using cytogenetics口頭発表（一般）
- 12th International Symposium Exploring the Global Sustainability, 2019年, 英語Epigenetical chromatin dynamics under abiotic stresses in rice.[招待有り]口頭発表（招待・特別）
- iJaDe2018, 2018年09月, 英語, Kobe, Japan, 国際会議Studies of histone modification caused by iron stress in tolerant and sensitive rice varieties口頭発表（一般）
- iJaDe2018, 2018年09月, 英語, Kobe, Japan, 国際会議Histone dynamics of single cell in plant development and environment[招待有り]口頭発表（招待・特別）
- 2nd e-ASIA Symposium on Functional Nano-Biology, 2018年09月, 英語, Osaka, Japan, 国際会議Chromosome mapping for rice genetics and breeding[招待有り]口頭発表（招待・特別）
- GSA/APCC6, 2018年07月, 英語, Canberra, Austraria, 国際会議Molecular Cytogenetics for Plant Genetics and Breeding[招待有り]口頭発表（招待・特別）
- GSA/APCC6, 2018年07月, 英語, Canberra, Austraria, 国際会議Cytological effect of histone modification and retrotransposon transcription by iron stress in riceポスター発表
- 一般社団法人園芸学会平成3 0 年度春季大会, 2018年03月, 日本語, 近畿大学, 国内会議ホウレンソウの雄決定遺伝子座周辺から見出された組換抑制領域の分子構造ポスター発表
- 鳥取大学乾燥地研究センター共同研究発表会, 2017年12月, 日本語, 鳥取大学, ジャトロファ（Jatropha curcas L.）は、トウダイグサ科に属するメキシコ原産の作物である。近年では、遺伝子組換え法を用いて、環境耐性を付与したジャトロファの作出が行われている。しかしながら、ジャトロファの組換え体作製に関して、安定化した植物体を作出し、育成するための基礎データの蓄積は未だ十分ではない。そこで、遺伝子組換え体において導入遺伝子がゲノムに導入されているか、どのように導入されているか、安定的に遺伝子発現しているかについて評価した。今年度は、β-グルクロニダーゼ（GUS）遺伝子導入個体ならびに、コエンザイムAの生合成に関わる酵素遺伝子であるシロイヌナズナ由来のPPAT遺伝子導入個体を対象に、１．蛍光in situハイブリダイゼーション（FISH）法を用いた遺伝子組換えによる外来遺伝子の導入様式と染色体解析、２.遺伝子発現の細胞内, 国内会議ジャトロファの遺伝子組換え体の繁殖ならびに染色体に関する研究ポスター発表
- 第10回国際環境生物資源学会, 2017年03月, 英語, 大阪大学, In this study, we investigated polyploidy level and chromosome structure using chromosome morphological analysis, fluorescence in situ hybridization (FISH), and chromosome image analyzing system (CHIAS) in Kudzu and Pyrethrum., 国際会議Effective chromosome research for inedible biomass plants口頭発表（一般）
- 財団法人日本染色体学会 第67回年会, 2016年11月, 日本語, 東京大学, 植物器官の構造を維持したまま細胞内の核酸やタンパク質を蛍光観察し、その局在性を捉えることは容易ではない。本研究では、植物の根の構造を生体に近い状態で保持したまま、内部構造を3次元的に高感度に画像解析する技術の開発を目指す。, 国内会議植物の3次元高感度イメージングによる局在解析口頭発表（一般）
- 財団法人日本染色体学会 第67回年会, 2016年11月, 日本語, 東京大学, 除虫菊ゲノム研究の基礎情報となる染色体に注目し、ピレスリン生合成系に関連する遺伝子解析を進めていく上で重要な役割を果たすと考えられる除虫菊の染色体情報を確立することを本研究の目的とした。実験手法としては、基本染色体数、倍数性の調査を行い、蛍光 in situ ハイブリダイゼーション（FISH）法を用いて、rDNAの位置情報とともに染色体の構造を明らかにした。, 国内会議除虫菊染色体の基礎研究口頭発表（一般）
- German-Japanese Symposium, Bridge Dresden-Japan, 2016年05月, 英語, Technische Universität Dresden, 国際会議Perspective of effective plant usages for edible and energy plants[招待有り]口頭発表（招待・特別）
- German-Japanese Symposium, Bridge Dresden-Japan, 2016年05月, 英語, The Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany, 国際会議Chromatin modification and mobility in plant development[招待有り]口頭発表（招待・特別）
- 第9回国際環境生物資源学会, 2016年03月, 英語, 大阪大学, In this study, we have expected early flowering and were intended to contribute to the effect Jatropha breeding. We performed three experimental trails. First, we constructed explants introduced Flowering Locus T gene and established the transformation and effective culture method., 国際会議Transgenic flowering gene and chromosome research in Jatropha口頭発表（一般）
- 第129回日本育種学会, 2016年03月, 日本語, 横浜市立大学, チャ（Camellia sinensis，2n=30）の変異拡大を目的とした近縁種との交雑における染色体の挙動解析のために，基本的な核型分析および減数分裂の対合を観察した．まずランドマークとして用いるリボゾーム遺伝子の多様性および染色体構成を調査するために，rDNAシークエンスおよび染色体fluorescence in situ hybridization(FISH)を比較した．またCamellia属植物3種において，チャカフェイン合成酵素遺伝子(TCS1)の染色体上での位置の特定を試みた．, 国内会議Camellia属植物におけるリボゾーム遺伝子の多様性と染色体構成口頭発表（一般）
- II International Symposium on Pyrethrum, 2015年08月, 英語, Kyoto, Japan, A cultivar of Tanacetum cinerariifolium, Pyrethrum in Asteraceae is used for the natural insecticide product, Pyrethrin for long period before the industrial chemical agents. However, its genome study has just barely started. In this study, we investigated polyploidy level and chromosome structure using chromosome morphological analysis, fluorescence in situ hybridization (FISH, 国際会議Chromosome Study of Pyrethrumポスター発表
- Asia Chromosome Colloquium, 2015年05月, 英語, Bangkok, Thai lands, Jatropha curcas L is a monoecious plant. But the ratio of female flower is less than the male flower. So the productivity falls and the yield is limited. So the study of a flower is important to improve epiphytic of fruit and yield. In this study, we generated early flowering transgenic Jatropha by introducing FLOWERING LOCUS T (FT) gene which is flowering hormone using agrobac, 国際会議Research of early flowering transgenic Jatropha plantポスター発表
- Asia Chromosome Colloquium, 2015年05月, 英語, Bangkok, Thai lands, A new cultivar of Brassica napus “Hanakkori” (AACC, 2n = 38) was resynthesized by hybridization and diplodization between B. rapa (AA, 2n = 20) and B. oleracea (CC, 2n = 18) in Yamaguchi, Japan. However, its genome instability has been noticed as shown in other resynthesized B. napus cultivars. In fact, when out-crossed seeds of Hanakkori are germinated, various kinds of morpho, 国際会議Chromosome instability of allopolyploid resynthesized Brassica napus[招待有り]シンポジウム・ワークショップパネル（指名）
- 8th International Symposium Exploring the global sustainability-Advances in Plant Biotechnology for Agriculture in Semi-arid land-, 2015年03月, 英語, Osaka Univ, 国際会議Research of early flowering transgenic Jatropha plant口頭発表（一般）
- 第127回日本育種学会, 2015年03月, 日本語, 玉川大学, 国内会議Camellia属植物および種間雑種におけるrDNA配列FISHの比較口頭発表（一般）
- 第37回日本分子生物学会, 2014年11月, 日本語, 横浜, 国内会議系統的に異なるSpinacia属植物からの同型および異型性染色体の同定口頭発表（一般）
- 第65回財団法人日本染色体学会, 2014年10月, 日本語, 岡山大学, 国内会議画像解析によるイネ染色体統合地図の構築口頭発表（一般）
- 第65回財団法人日本染色体学会, 2014年10月, 英語, 岡山大学, 国内会議Chromosome instability in the progeny of amphidiploid resynthesized Brassica napusポスター発表
- 第125回日本育種学会, 2014年03月, 日本語, 東北大学, 国内会議サトウキビとエリアンサスの属間雑種の細胞遺伝学的特性および農業形質の評価口頭発表（一般）
- 第125回日本育種学会, 2014年03月, 日本語, 東北, 国内会議Spinacia属から見出された同型および異型性染色体のFISH法による特徴付け口頭発表（一般）
- 7th meeting of international society for environmental bio-Resources, 2014年03月, 英語, Osaka Univ, 国際会議Generation of early flowering transgenic plant and study of flower development in Jatropha.[招待有り]口頭発表（招待・特別）
- Chromosome imaging workshop, 2014年02月, 英語, Oxford Univ.UK., 国際会議Divergence of plant genome revealed by FISH[招待有り]口頭発表（招待・特別）
- 平成２５年度鳥取大学乾燥地研究センター共同研究発表会, 2013年12月, 日本語, 鳥取大学, 国内会議ジャトロファの早期開花組換えならびに花成に関する研究ポスター発表
- 染色体学会, 2013年11月, 日本語, 財団法人染色体学会, 富山大学, 国内会議日本に自生するクズの核型解析口頭発表（一般）
- 6th International Symposium Green Biotechnology for Global Sustainability, 2013年03月, 英語, Osaka Univ, 国際会議Production of transgenic plants with early flowering gene and morphological analysis of flowers in Jatoropha口頭発表（一般）
- 鳥取大学乾燥地研究センター発表会, 2012年12月, 日本語, 鳥取大学乾燥地研究センター, 国内会議油糧植物ジャトロファの早期開花組換え体創出に関する研究ポスター発表
- 日本育種学会, 2012年11月, 日本語, 京都産業大学, 国内会議バイオ燃料植物ジャトロファの開花遺伝子ならびに組換えに関する研究口頭発表（一般）
- International Conference on NextGeneration Technologies for Bioenergy and Biomass Utilisation, 2012年08月, 英語, Singapore, 国際会議Development of Jatropha transgenic plants with drought tolerance.[招待有り]口頭発表（招待・特別）
- 平成23年度日本理科教育学会近畿支部大会発表論文集, 2011年11月, 日本語, 国内会議アーギュメンテーション・スキルの育成を目指した実践的研究:小学校第6学年の理科授業デザイン口頭発表（一般）
- 2008年度日本女性学会大会, 2008年06月, 日本語, アピオ青森, 国内会議大学職場の男女平等実現のためのストラテジー口頭発表（一般）
- 第５８回染色体学会, 2007年11月, 日本語, 総合研究大学院大学 葉山キャンパス, 国内会議アカクローバー完全染色体地図の構築口頭発表（一般）
- 第５８回染色体学会, 2007年11月, 日本語, 総合研究大学院大学 葉山キャンパス, 国内会議CHIAS4を用いたアカクローバー染色体の画像解析法の確立口頭発表（一般）
- 日本教育工学会第23回, 2007年09月, 日本語, 早稲田大学, 国内会議学校と専門家を結ぶケータイと電子掲示板を使った連携授業：小学校2年生の生活科における冬野菜の栽培活動口頭発表（一般）
- 第１６回国際染色体会議, 2007年08月, 英語, オランダ・アムステルダム, 国際会議Quantitative and fine rice pachytene chromosome map by analyzed chromomeric featuresポスター発表
- 第１６回国際染色体会議, 2007年08月, 英語, 16th International Chromosome Conference, オランダ・アムステルダム, 国際会議Legume chromosome studies of Trifolium pretense and Lotus japonicusポスター発表
- 第5回国際牧草分子生物学会, 2007年08月, 英語, 札幌市, 国際会議Chromosome map analysis of Red cloverポスター発表
- 染色体学会第57回大会, 2006年11月, 日本語, 千葉大学, 国内会議マメ科植物染色体の定量的マッピングの進展口頭発表（一般）
- 染色体学会第57回大会, 2006年11月, 日本語, 千葉大学, 国内会議カプセル化法を用いた植物培養細胞の凍結保存口頭発表（一般）
- 染色体学会第57回大会, 2006年11月, 日本語, 千葉大学, 国内会議アカクローバーにおける連鎖地図と染色体地図の統合口頭発表（一般）
- 日本育種学会第110回大会, 2006年09月, 日本語, 愛媛大学, 国内会議タバコならびにイネ培養細胞のカプセル化による効果的な凍結保存法口頭発表（一般）
- 平成１７年度日本理科教育学会近畿支部大会, 2005年11月, 日本語, 滋賀大学, 国内会議遺伝子組み換え食品を題材にしたCSCLシステム活用型科学教育カリキュラム：実験授業に参加した科学者が語るカリキュラムの意義口頭発表（一般）
- 平成１７年度日本理科教育学会近畿支部大会, 2005年11月, 日本語, 滋賀大学, 国内会議ケータイを活用した学校・農園連携授業の試み：小学校２年生の生活科「冬の野さいをそだてよう」口頭発表（一般）
- 日本科学教育学会第29回年会, 2005年09月, 日本語, 日本科学教育学会, 岐阜, 日本, 国内会議遺伝子組換え食品問題に対する社会的意思決定をテーマとしたCSCLシステム活用型科学教育カリキュラム：獲得した意思決定能力は他の文脈でも発揮されるか？口頭発表（一般）
- 日本科学教育学会第29回年会, 2005年09月, 日本語, 日本科学教育学会, 岐阜, 日本, 国内会議遺伝子組換え食品問題に対する社会的意思決定をテーマとしたCSCLシステム活用型科学教育カリキュラム：2004年度版カリキュラムを学習した小学生の社会的意思決定の達成度口頭発表（一般）
- 日本育種学会 第107・108回講演会, 2005年08月, 日本語, 日本育種学会, Tsukuba, 日本, 国内会議マメ科植物ミヤコグサの定量的染色体地図と連鎖地図の統合口頭発表（招待・特別）
- 平成１７年度学部附属共同研究教育研究発表会, 2005年06月, 日本語, 神戸大学発達科学部附属住吉小学校, 国内会議ITによる協調学習支援ー自然・社会認識におけるITを活用した協調学習の授業デザインその他
- 平成16年度日本理科教育学会近畿支部大会, 2004年11月, 日本語, 日本理科教育学会近畿支部, 大阪, 日本, 国内会議Knowledge Forumを利用した学習環境のデザイン実験：遺伝子組換え食品を題材にした単元の開発IIIポスター発表
- 日本科学教育学会研究会研究報告, 2004年10月, 日本語, 日本科学教育学会, 国内会議CSCLシステムを利用した授業のデザイン実験口頭発表（一般）
- 日本遺伝学会 第76会大会, 2004年09月, 日本語, 日本遺伝学会, Suita, 日本, 国際会議植物細胞周期を通じたHeterochromatin Protein 1 ならびにヒストンH3メチル化の局在性口頭発表（一般）
- International workshopCell and Molecular Biology of Tobacco BY-2 cells BY 2, 2004年09月, 英語, Yokohama, 日本, 国際会議Intercelluler dynamics of heterochromatin protein in BY-2 cell cycle.ポスター発表
- 日本育種学会 第106回講演会, 2004年09月, 日本語, 日本育種学会, Tsu, 日本, 国内会議FISHならびにCHIAS3を用いたミヤコグサ定量的パキテン染色体地図の構築口頭発表（一般）
- 日本科学教育学会第28回年会, 2004年08月, 日本語, 日本科学教育学会, 千葉, 日本, 国内会議遺伝子組み換え食品問題に対する社会的意思決定をテーマとした科学教育のためのCSCL環境：社会的意思決定の達成度に関する分析口頭発表（一般）
- 日本科学教育学会第28回年会, 2004年08月, 日本語, 日本科学教育学会, 千葉, 日本, 国内会議遺伝子組み換え食品問題に対する社会的意思決定をテーマとした科学教育のためのCSCL環境：基礎的内容の理解度口頭発表（一般）
- 日本科学教育学会第28回年会, 2004年08月, 日本語, 日本科学教育学会, 千葉, 日本, 国内会議遺伝子組み換え食品問題に対する社会的意思決定をテーマとした科学教育のためのCSCL環境：概念的理解とイメージの変容口頭発表（一般）
- The 2nd asian chromsome colloquium, 2004年05月, 英語, 太田, 韓国, 国際会議High resolution chromosome mapping of Lotus japonicus口頭発表（一般）
- 日本教育工学会第19回全国大会, 2003年10月, 日本語, 日本教育工学会, 盛岡, 日本, 国内会議遺伝子組み換え食品問題に対する社会的意思決定をテーマとした科学教育のためのCSCL環境：学習者からみた有効性口頭発表（一般）
- 日本育種学会 第104回講演会, 2003年09月, 日本語, 日本育種学会, Kobe, 日本, 国内会議反復配列ならびにTACクローンを用いたミヤコグサ染色体物理地図の作製口頭発表（一般）
- 日本理科教育学会第53回全国大会, 2003年08月, 日本語, 日本理科教育学会, 札幌, 日本, 国内会議Knowledge Forumを利用した学習環境のデザイン実験：遺伝子組み換え食品を題材にした単元の開発II口頭発表（一般）
- The 1st asian chromsome colloquium, 2001年09月, 英語, Beijing, 中国, 国際会議High resolution FISH for gene mapping and molecular analysis of rice chromosomes口頭発表（一般）
- 東京大学分子細胞生物学研究所セミナー, 2001年03月, 日本語, 東京大学, Tokyo, 日本, 国内会議遺伝子高感度可視化技術によるゲノム解析口頭発表（招待・特別）
- Plant and Animal Genome IX, 2001年01月, 英語, Schergo International,Inc, SanDiego, アメリカ, 国際会議Genome size of the genus Fragariaポスター発表
- 第10回 染色体コロキュウム, 2000年12月, 日本語, かずさDNA研究所, 木更津, 日本, 国内会議高感度可視化技術を用いた遺伝子の検出口頭発表（招待・特別）
- 第23回 日本分子生物学会年会, 2000年12月, 日本語, 日本分子生物学会, Kobe, 日本, 国内会議FISH法を用いた植物ゲノムにおける遺伝子の可視化口頭発表（一般）
- 第51回 染色体学会, 2000年10月, 日本語, （財）染色体学会, Yokohama, 日本, 国内会議カスタムの標識ヌクレオチドを用いたマルチカラーFISH法の開発口頭発表（一般）
- 4th International Rice Genetics Symposium, 2000年10月, 英語, International Rice Research Insutitute, Laguna, フィリピン, 国際会議High resolution FISH for gene mapping and molecular analysis of rice chromosomes口頭発表（一般）
- 日本育種学会 第98回講演会, 2000年09月, 日本語, 日本育種学会, Hirosaki, 日本, 国内会議画像解析によるイネ第９染色体パキテン期高精度定量的染色体地図の作成口頭発表（一般）
- 日本育種学会 第98回講演会, 2000年09月, 日本語, 日本育種学会, Hirosaki, 日本, 国内会議フローサイトメトリーを用いたイチゴゲノムサイズの解析口頭発表（一般）
- 大阪大学大学院工学研究科応用生物セミナー, 2000年07月, 日本語, 大阪大学, Suita, 日本, 国内会議遺伝子の可視化ー染色体から１塩基まで口頭発表（招待・特別）
- 日本育種学会 第97回講演会, 2000年04月, 日本語, 日本育種学会, Tsukuba, 日本, 国内会議Rolling circle amplification (RCA)法によるDNA配列変異の高感度検出．口頭発表（一般）
- 園芸学会 平成12年度春季大会研究発表, 2000年03月, 日本語, 園芸学会, Kobe, 日本, 国内会議高再分化能ハクサイ‘Homei’およびキャベツ‘松波’小胞子由来再分化植物の染色体数.口頭発表（一般）
- Plant and Animal Genome VIII, 2000年01月, 英語, Scherago International,Inc., SanDiego, アメリカ, 国内会議BRYOPHYTE 5S RDNA WAS INSERTED INTO 45S RDNA REPEAT UNITS AFTER THE DIVERGENCE FROM HIGHER LAND PLANTSポスター発表
- 染色体学会 第50回記念大会, 1999年11月, 日本語, （財）染色体学会, Nishinomiya, 日本, 国内会議植物染色体をのばしてみる-染色体FISHからDNAファイバーFISHへ-口頭発表（一般）
- 日本育種学会 第96回講演会, 1999年09月, 日本語, 日本育種学会, Okayama, 日本, 国内会議Nicotiana tabacum L.およびその近縁野生種の染色体におけるリボソームRNA遺伝子のマッピング口頭発表（一般）
- 日本育種学会 第95回講演会, 1999年04月, 日本語, 日本育種学会, Utsunomiya, 日本, 国内会議走査型プローブ顕微鏡による染色体情報の解析と利用1.原子間力顕微鏡(AFM)によるEDF法標本不のDNAファイバーの観察口頭発表（一般）
- 日本育種学会 第95回講演会, 1999年04月, 日本語, 日本育種学会, Utsunomiya, 日本, 国内会議染色体情報の解析・利用に関する研究119.アラビドプシス染色体の定量的地図の作製口頭発表（一般）
- 日本育種学会 第95回講演会, 1999年04月, 日本語, 日本育種学会, Utsunomiya, 日本, 国内会議イネの動原体領域に由来するYACクローンの構造解析口頭発表（一般）
- 日本育種学会 第95回講演会, 1999年04月, 日本語, 日本育種学会, Utsunomiya, 日本, 国内会議Solanum integrifolium とS. sanitwongseiの６倍性体細胞雑種の染色体構成と形態特性について口頭発表（一般）
- 日本育種学会 第95回講演会, 1999年04月, 日本語, 日本育種学会, Utsunomiya, 日本, 国内会議5SrDNAを用いたFISHによるNicotiana kawakamii Y. Ohashiの小型染色体の同定口頭発表（一般）
- 第16回 染色体ワークショップ, 1999年01月, 日本語, 日本,, 国内会議アラビドプシスにおける染色体の定量的地図の作成口頭発表（一般）
- Plant and Animal Genome VII, 1999年01月, 英語, Scherago International,Inc., SanDiego, アメリカ, 国際会議Visualization of the DNA stretches in a rice genome by extended DNA fiber-FISH.口頭発表（一般）
- Plant and Animal Genome VII, 1999年01月, 英語, Scherago International,Inc., SanDiego, アメリカ, 国際会議Development of a quantitative chromosome map of Arabidopsis by imaging methods.口頭発表（一般）
- Plant and Animal Genome VII, 1999年01月, 英語, Scherago International,Inc., SanDiego, アメリカ, 国際会議A new method in optical mapping visualizes the structure of the S locus region of Brassica campestris L.口頭発表（一般）
- 第21回 日本分子生物学会年会, 1998年12月, 日本語, 日本分子生物学会, Yokohama, 日本, 国内会議染色体情報の解析・利用に関する研究 118. Fiber-FISH法によるゲノム内部構造の可視化解析．ポスター発表
- 日本育種学会 第94回講演会, 1998年09月, 日本語, 日本育種学会, Morioka, 日本, 国内会議染色体情報の解析・利用に関する研究117. ホウレンソウにおける染色体凝縮パターンに基づく核型解析.口頭発表（一般）
- 日本育種学会 第94回講演会, 1998年09月, 日本語, 日本育種学会, Morioka, 日本, 国内会議染色体情報の解析・利用に関する研究116. DNAcombing法による定量的FISH法の開発．口頭発表（一般）
- 日本育種学会 第94回講演会, 1998年09月, 日本語, 日本育種学会, Morioka, 日本, 国内会議タバコTゲノム染色体の分析と比較口頭発表（一般）
- 13th International Chromosome Conference, 1998年09月, 英語, Cytogenetics and Cell Genetics, Ancona, イタリア, 国際会議Paralleled changes of repetitive sequences in the Oryza genomes.ポスター発表
- 日本育種学会 第94回講演会, 1998年09月, 日本語, 日本育種学会, Morioka, 日本, 国内会議FISH法によるダイズとツルマメにおける染色体不のrDNAの位置の比較.口頭発表（一般）
- 日本育種学会 第94回講演会, 1998年09月, 日本語, 日本育種学会, Morioka, 日本, 国内会議CMA/DAPI染色およびFISHによるNicotiana Kawakamii Y. Ohashiの核型分析口頭発表（一般）
- The 18th International Congress of Genetics, 1998年08月, 英語, Chinese Academy of Sciences, Genetics Society of China, Beijing, 中国, 国際会議Visual verification of the telomeric structure of the rice chromosomes口頭発表（一般）
- 日本植物生理学会 1998年度年会, 1998年05月, 日本語, 日本植物生理学会, Hokkaodo, 日本, 国内会議イネの伸長DNAファイバーを用いた高解像FISH法による遺伝子コピー数の推定口頭発表（一般）
- 日本育種学会 第93回講演会, 1998年04月, 日本語, 日本育種学会, Yokohama, 日本, 国内会議染色体情報の解析・利用に関する研究114. EDFs-FISH法による遺伝子コピー数の定量化技術の開発.口頭発表（一般）
- 日本育種学会 第93回講演会, 1998年04月, 日本語, 日本育種学会, Yokohama, 日本, 国内会議染色体情報の解析・利用に関する研究113.DNAファイバー不でのシングルコピー遺伝子の可視化．口頭発表（一般）
- 日本育種学会 第93回講演会, 1998年04月, 日本語, 日本育種学会, Yokohama, 日本, 国内会議Festuca-Lolium属間雑種F2世代の表現型と染色体異常口頭発表（一般）
- Plant and Animal Genome VI, 1998年01月, 英語, Scherao International,Inc., SanDiego, アメリカ, 国際会議Analysis and utility of chromosome information 110. Detection of specific chromosome reduction in rice somatic hybrids with A, B and C genome by multi-color genomic in situ hybridization.口頭発表（一般）
- Plant and Animal Genome VI, 1998年01月, 英語, Scherao International,Inc., SanDiego, アメリカ, 国際会議Analysis and utility of chromosome information 109. Molecular cytogenetical characteristics of rice tissue culture cells with high activity of retrotransposon.口頭発表（一般）
- Plant and Animal Genome VI, 1998年01月, 英語, Scherao International,Inc., SanDiego, アメリカ, 国際会議Advances in high resolution FISH using extended DNA fibers in plants.口頭発表（一般）
- 第20回 日本分子生物学会年会, 1997年12月, 日本語, 日本分子生物学会, Kyoto, 日本, 国内会議染色体情報の解析と利用に関する研究108.Spectral-FISHによるイネ染色体の同定ポスター発表
- 日本育種学会 第92回講演会, 1997年10月, 日本語, 日本育種学会, Tottori, 日本, 国内会議染色体情報の解析・利用に関する研究107.超高解像度FISH法によるB. campestrisのリボソームRNA遺伝子構造の可視化口頭発表（一般）
- 日本育種学会 第92回講演会, 1997年10月, 日本語, 日本育種学会, Tottori, 日本, 国内会議染色体情報の解析・利用に関する研究106.Javaを用いた定量的染色体地図の作成口頭発表（一般）
- 日本育種学会 第92回講演会, 1997年10月, 日本語, 日本育種学会, Tottori, 日本, 国内会議Nicotiana tabacum L.とN.kawakami Y. Ohashiとの種間雑種におけるGISHで得られるシグナルパターンの分析口頭発表（一般）
- 日本植物学会 第61回大会研究発表, 1997年09月, 日本語, 日本植物学会, Narashino, 日本, 国内会議染色体情報の利用と解析に関する研究105. DNAファイバー不でのFISH法およびその定量的解析．口頭発表（一般）
- 5th International Congress of Plant Molecular Biology, 1997年09月, 英語, The International Society For Plant molecular Bioligy,The National Univercity Of singapore,The Insutitute Of Molecular Agrobiology, Singapore, シンガポール, 国際会議RFLP analysis and genomic in situ hybridization (GISH) in somatic hybrids and progenies between Lycopersicon esculentum and Solanum lycopersicoides.ポスター発表
- 5th International Congress of Plant Molecular Biology, 1997年09月, 英語, The International Society For Plant molecular Bioligy,The National Univercity Of singapore,The Insutitute Of Molecular Agrobiology, Singapore, シンガポール, 国際会議Physical and Quantitative localisation of repetitive sequences in Oryza Species.ポスター発表
- 5th International Congress of Plant Molecular Biology, 1997年09月, 英語, The International Society For Plant molecular Bioligy,The National Univercity Of singapore,The Insutitute Of Molecular Agrobiology, Singapore, シンガポール, 国際会議Localization of rice 5S-rRNA genes on extended DNA fibers.[招待有り]口頭発表（招待・特別）
- 日本植物学会 第61回大会研究発表, 1997年09月, 日本語, 日本植物学会, Narashino, 日本, 国内会議Brachyscome lineariloba複合体(2n=4,8,10,12,16)におけるr-DNAの染色体不の分布．口頭発表（一般）
- Exchange Center, 1997年03月, 日本語, 日本育種学会, Tokyo, 日本, 国内会議染色体情報の解析・利用に関する研究 101. イネの特異的反復配列（TrsA）およびテロメア配列のDNAファイバー不での可視化．口頭発表（一般）
- 日本育種学会 第91回講演会, 1997年03月, 日本語, 日本育種学会, Tokyo, 日本, 国内会議植物染色体におけるトポイソメラゼ-IIの分布解析．口頭発表（一般）
- International Workshop on Analysis and Utility of Plant Chromosome Information, 1997年03月, 英語, Hokuriku National Agricuitural Experiment Atation,Japan, Science and Technology Agency,Japan, Japan International Science and Technology, Niigata, 日本, 国際会議Variation in genome size and chromosome morphology due to repetitive sequences in the genus Oryza.口頭発表（一般）
- International Workshop on Analysis and Utility of Plant Chromosome Information, 1997年03月, 英語, Hokuriku National Agricuitural Experiment Atation,Japan, Science and Technology Agency,Japan, Japan International Science and Technology, Niigata, 日本, 国際会議The mechanism of chromosome structure variation in cultured cells and at first mitoses in germinating aged seeds of maize.口頭発表（一般）
- 39th annual maize genetics conference, 1997年03月, 英語, Florida, アメリカ, 国際会議The distribution of topoisomerase II on maize somatic chromosomes.ポスター発表
- International Workshop on Analysis and Utility of Plant Chromosome Information, 1997年03月, 英語, Hokuriku National Agricuitural Experiment Atation,Japan, Science and Technology Agency,Japan, Japan International Science and Technology, Niigata, 日本, 国際会議Quantitative chromosome mapping of three diploid genomes of the genus Brassica.口頭発表（一般）
- International Workshop on Analysis and Utility of Plant Chromosome Information, 1997年03月, 英語, Hokuriku National Agricuitural Experiment Atation,Japan, Science and Technology Agency,Japan, Japan International Science and Technology, Niigata, 日本, 国際会議Phyletic relationship of races and genomic reorganization in the Brachyscome lineariloba Complex.口頭発表（一般）
- 日本育種学会 第91回講演会, 1997年03月, 日本語, 日本育種学会, Tokyo, 日本, 国内会議Nicotiana tabacum L.とN.kawakamii Y.Ohashiとの種間雑種におけるGISH法を用いた染色体分析口頭発表（一般）
- International Workshop on Analysis and Utility of Plant Chromosome Information, 1997年03月, 英語, Hokuriku National Agricuitural Experiment Atation,Japan, Science and Technology Agency,Japan, Japan International Science and Technology, Niigata, 日本, 国際会議High resolution physical mapping in rice.口頭発表（一般）
- 日本育種学会 第91回講演会, 1997年03月, 日本語, 日本育種学会, Tokyo, 日本, 国内会議Festuca-Lolium属間F1雑種の異ゲノム接合とF2世代の相互転座口頭発表（一般）
- International Workshop on Analysis and Utility of Plant Chromosome Information, 1997年03月, 英語, Hokuriku National Agricuitural Experiment Atation,Japan, Science and Technology Agency,Japan, Japan International Science and Technology, Niigata, 日本, 国際会議Evidence for diploidization of tetraploid burnet (Sanguisorba L., ROSACEAE).口頭発表（一般）
- International Workshop on Analysis and Utility of Plant Chromosome Information, 1997年03月, 英語, Hokuriku National Agricuitural Experiment Atation,Japan, Science and Technology Agency,Japan, Japan International Science and Technology, Niigata, 日本, 国際会議Distinction of parental chromosomes in somatic hybrids between Solanum integrifolium and S. sanitwongsei using genomic in situ hybridization (GISH).口頭発表（一般）
- International Workshop on Analysis and Utility of Plant Chromosome Information, 1997年03月, 英語, Hokuriku National Agricuitural Experiment Atation,Japan, Science and Technology Agency,Japan, Japan International Science and Technology, Niigata, 日本, 国際会議Direct measurement of 5s ribosomal RNA gene by FISH on extended DNA fibers.口頭発表（一般）
- Plant & Animal Genome V, 1997年01月, 英語, Scherao International,Inc., SanDiego, アメリカ, 国際会議Quantitative chromosome mapping of three diploid genomes of the genus Brassica.ポスター発表
- Plant & Animal Genome V, 1997年01月, 英語, Scherao International,Inc., SanDiego, アメリカ, 国際会議Quantitative analysis of fluorescence in situ hybridization to extended DNA fibers.ポスター発表
- Aberystwhty cell genetics group 7th annual conference, Physical mapping of plant chromosomes, 1997年01月, 英語, Aberystwhty cell genetics group, Aberystwyth, イギリス, 国際会議Physical mapping of single copy genes using an improved FISH method in rice口頭発表（一般）
- Plant & Animal Genome V, 1997年01月, 英語, Scherao International,Inc., SanDiego, アメリカ, 国際会議Newly propozed basic chromosome number in burnet (Sanguisorba L., rosaceae): evidence from number of 5S rDNA loci detected by FISH.ポスター発表
- Plant & Animal Genome V, 1997年01月, 英語, Scherao International,Inc., SanDiego, アメリカ, 国際会議Immunofluorescence analysis of the distribution of methylated cytosine in maize and rice somatic chromosomesポスター発表
- Plant & Animal Genome V, 1997年01月, 英語, Scherao International,Inc., SanDiego, アメリカ, 国際会議High resolution physical mapping gene by McFISH in rice.ポスター発表
- ISRMB'96 Fifth International Sympsium on Rice Molecular Biology, 1996年10月, 英語, ISRMB, Taipei, 台湾, 国際会議The distribution of 5-methylcytosine in somatic chromosomes of germinating aged rice seeds.ポスター発表
- The Ministry of Education Science, Sports and Culture, 1996年, 英語, The Ministry of Education Science, Sports and Culture, Sendai, 日本, 国際会議Visualization of methylated cytosine in maize somatic chromosomes口頭発表（一般）
■ 共同研究・競争的資金等の研究課題
- 日本学術振興会, 二国間交流事業共同研究・セミナー, DAAD, 神戸大学, 2020年04月 - 2022年03月, 研究代表者アジサイの相互補完的遺伝資源植物コレクションの構築
- 戦略的国際共同研究プログラム（SICORP）, 日本-タイ国・ベトナム国(革新的材料のための機能性バイオナノテクノロジー), 神戸大学, 2018年01月 - 2022年03月遺伝物質の構造および初期感染過程のナノ可視化法の開発によるバイオナノテクノロジーの新たな展開
- 日本学術振興会, 二国間交流事業共同研究・セミナー, CAS, 2018年04月 - 2020年03月, 研究代表者外来植物染色体添加系統における遺伝子発現の改変
- 日本学術振興会, 科学研究費助成事業, 基盤研究(A), 2013年04月01日 - 2018年03月31日FIB/SEMを用いたヌクレオソームトモグラフィーによる染色体内部構造の解明３世紀に渡って謎のままである染色体の内部構造について、ヌクレオソームの位置を可視化することにより明らかにすることを試みた。その為、イオンビームを用いて染色体のスライスを作製し、その断面像を画像解析した。その結果、ヌクレオソームの配列に規則性は見出せず、染色体内部でヌクレオソームはほぼランダムに分布していると考えられた。同時に行った染色体軸の解析では、複数のタンパク質の軸が互いに絡み合っていることを見出し、剛性と柔軟性を共に兼ね備えた構造であることを明らかにした。
- 学術研究助成基金助成金／挑戦的萌芽研究, 2016年04月 - 2018年03月競争的資金
- 日本学術振興会, 科学研究費助成事業, 基盤研究(B), 北海道大学, 2014年04月01日 - 2017年03月31日ホウレンソウ性決定遺伝子座の構造決定および進化過程の解明ホウレンソウはアカザ科の雌雄異株として知られるが，間性株も見られる．雌雄の決定は性染色体(XY)に座乗する遺伝子ペア(YおよびX) に支配される．間性発現を支配する遺伝子（M）は，Y座と約12 cM隔てて連鎖している．本研究では，ホウレンソウ性染色体をカバーする108 cMの分子連鎖地図を構築した．次いで，Y座に連鎖する220個の遺伝子を同定し，これらの一部は組換え抑制領域に座乗している可能性を示す証拠を得た．アカザ科のテンサイゲノムとの比較から，ホウレンソウの性決定遺伝子はペリセントロメア領域に座乗している可能性が示された．間性遺伝子座乗候補領域を約25 kbpに絞り込んだ．
- 日本学術振興会, 科学研究費助成事業, 基盤研究(B), 神戸大学, 2014年04月01日 - 2017年03月31日トランス・サイエンス問題の解決能力を育成する知識共創型アーギュメンテーション教育トランス・サイエンス問題の解決能力について，科学的探究能力の育成，科学的アーギュメンテーションの理解，科学技術を扱うトランス・サイエンス問題に対する思考の評価，の3点から取り組んだ。思考の評価については，科学技術の社会問題を取り上げ，先進的な指導事例や，解決能力の測定課題と評価法に関する実証的知見を蓄積し，指導法と評価法の体系的理論化をはかった。検討結果を元に作成した測定課題を用いて調査を実施し，トランス・サイエンス問題に対する大学生の思考の特徴を明らかにするとともに，評価用のルーブリックを精緻化した。また，発話分析から，科学的アーギュメンテーションの認識論的理解の獲得過程を解明した。
- 国立研究開発法人科学技術振興機構, 国際科学技術共同研究プログラム(SICORP), 2017年, 研究代表者遺伝物質の構造のナノ可視化法の開発によるバイオナノテクノロジーの新たな展開競争的資金
- 科学研究費一部基金／基盤研究(B)特設, 2013年04月 - 2016年03月競争的資金
- 科学研究費補助金／基盤研究(B), 2012年04月 - 2015年03月競争的資金
- 学術研究助成基金助成金／挑戦的萌芽研究, 2011年競争的資金
- 日本学術振興会, 科学研究費助成事業, 特定領域研究, 独立行政法人農業生物資源研究所, 2008年 - 2009年ミヤコグサとのシンテニーを利用したダイズのゲノム構造の解析1. ミスズダイズと秣食豆公503に由来するRILの連鎖地図を基準にして、ミヤコグサの塩基配列情報を利用して比較地図を作製した。F2連鎖地図を用いた結果と同様にダイズの染色体はミヤコグサ連鎖ブロックのモザイクとなっていた。相同染色体は1対ずつで、染色体レベルでは2倍体である。 2. Williams82のゲノム塩基配列から同祖領域を探索したところ、領域の大きさは最大約12.4MBにわたっていることがわかった。同祖領域の分布は極めて複雑でダイズゲノムは複数の倍加と再編成を経て形成されたと考えられる 3. ミヤコグサの花の対称性に関わる遺伝子cyc3のオーソログGmcyc3aを含むBACクローンとGmcyc3aの同祖遺伝子Gmcyc3bを含むBACクローンを異なる蛍光色素でラベルしてパキテン期染色体に同時に貼り付けるとそれぞれ同じ領域にシグナルが観察され、一方のシグナルが強いところは他方のシグナルが弱く見かけ上2つのシグナルがはっきりと認められた。同じことが、同祖遺伝子NFR1a、NFR1bなどでも観察された。また開花期関連遺伝子FT3を含むダイズのTACクローンと相同なミヤコグサのTACクローンは同じ位置にシグナルが見られ強弱が逆転していた。一般にミヤコグサのTACクローンはダイズのBACクローンに比較してシグナルが弱かった。 4. ダイズのゲノムはマメ科植物の中で、極めて特異的であることがわかった。マクロシンテニー、マイクロシンテニーの解析から、倍加と再編成の結果、現在のゲノム構造が生じたと考えられる。ホモロジーの極めて高い同祖遺伝子は、一般に2つ存在していてそれは最も新しい時期の倍加による結果だと考えられる。それらの遺伝子間では機能の分業が生じていて、ダイズは遺伝子レベルでも2倍体としてのシステムを獲得していると言える。
- 科学研究費補助金／基盤研究(C), 2008年, 研究代表者競争的資金
- 科学研究費補助金／萌芽研究, 2008年競争的資金
- 科学研究費補助金／基盤研究(C), 2006年競争的資金
- 2005年, 研究代表者染色体の構造と機能解明のためのナノデバイスに関する総合研究競争的資金
- 科学研究費補助金／基盤研究(C), 2005年, 研究代表者競争的資金
- 日本学術振興会, 科学研究費助成事業, 特定領域研究, 神戸大学, 2003年 - 2004年CSCLシステムを活用した科学教育カリキュラムの日本型モデル本年度の研究実績は,以下であった。 1.文献・資料収集とそのデータベース化 初年度に引き続き,協調的学習に関する理論や方法に関する研究及び情報帳信ネットワークの教育利用に関する研究を収集し,これまでの研究成果や議論を整理した。また,諸外国における研究の先進的な事例を調査するとともに,これら収集した資料をデータベース化し,WWW上で公開した。 2.日本型カリキュラム・モデル試案の検討 初年度に開発した暫定的なCSCLシステム及びカリキュラムのモデルを再検討するとともに,新しく遺伝子工学に関する小学生用の具体的な学習用コンテンツの制作を行った。 3.国内・国際学会等における中間的成果の発表 千葉大学で開催される日本科学教育学会第28回年会において,中間的な発表を行った。また,その他の日本教育工学会等で成果の一部を発表した。さらに,8月にカナダ・トロント大学の応用認知科学センターで開催されるKnowledge Forumの国際会議で発表を行い,北米における同様の研究との比較を試みる中で,本研究で開発したカリキュラムを日本型モデルとして提案した。 4.CSCLを利用した日本型モデルに基づいたカリキュラム開発,運用,評価,公開 3.で報告した学習環境(システム,学習用コンテンツ及びカリキュラム)の日本型モデルの一部を具体化し,神戸大学発達科学部の附属住吉小学校において,1クラス規模の実験授業を行った。また。2004年10月には日本科学教育学会研究会の際に,公開授業研究会を開催し,カリキュラムの普及を行った。本実験授業に即して作成した各種コンテンツ等については,NICERのサーバで広く公開することを予定している。