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高見 泰興大学院人間発達環境学研究科 人間環境学専攻教授
研究者基本情報
■ 学位■ 研究ニュース
■ 研究分野
■ 委員歴
研究活動情報
■ 受賞- 2024年03月 日本昆虫学会, 論文賞, Functional, genetic, and structural constraints on the exaggeration and diversification of male genital morphology in Ohomopterus ground beetles
- 2017年12月 日本動物行動学会, 第4回Journal of Etholgy論文賞, Male mate choice in a sexually cannibalistic species: male escapes from hungry females in the praying mantid Tenodera angustipennis
- 2000年 日本昆虫学会, 日本昆虫学会賞, Geographic variation in body size of some Japanese Leptocarabus species (Coleoptera, Carabidae): The "toppled-domino pattern" in species along a geographic cline
- The Arthropodan Embryological Society of Japan, 2025年, Proceedings of the Arthropodan Embryological Society of Japan, 55, 1 - 7, 英語[査読有り]研究論文（学術雑誌）
- Abstract The genus Carabus (Coleoptera: Carabidae: Carabinae) is a group of predacious ground beetles found throughout Holarctic temperate zones. The diversification of this group is attributed not only to allopatric speciation but also to diversification in life-history traits and morphological traits that are involved in ecological adaptation and reproductive isolation. Precise phylogenetic reconstruction of Carabus is important to understanding the factors associated with species’ divergence in relation to such character divergence. Here, we analyse the phylogeny of Carabus among higher taxonomic groups and among species of the subgenus Apotomopterus, a hyper-diverse group endemic to East Asia. We used genome-wide sequence data from ultraconserved elements for phylogenetic reconstruction via maximum-likelihood and species-tree reconstruction methods. With regard to the relationships among higher taxonomic groups, the obtained phylogenetic trees resolved two structures with previously unresolved divergence. The subgenus Apotomopterus contained three lineages, i.e. the Vitalisi, Prodigus, and Sauteri groups. It showed diversification of the spinula, a sclerotized male genital structure, and variation in the female elytral notch (preapical emargination). These morphological traits may be involved in copulatory behaviour and potentially affect reproductive isolation, and their character state divergence may have facilitated species divergence of Apotomopterus.Oxford University Press (OUP), 2024年10月, Zoological Journal of the Linnean Society, 202(2) (2), zlae125, 英語[査読有り]研究論文（学術雑誌）
- Abstract Changes in the morphology, physiology and behavior of parasitized organisms provide an ideal opportunity to examine the extended phenotype of parasites. Since the quality of the host directly affects the fitness of the parasite, parasites may increase their fitness by manipulating phenotypes of low-quality hosts. Males are usually preyed on by females in sexual cannibalism. Thus, the males of sexually cannibalistic species are unsafe and low-quality hosts for parasites, while females may be beneficial hosts because of the chance of nutrient intake from cannibalized males. Under passive modes of transmission, parasites cannot choose the host sex. Such parasites exploiting sexually cannibalistic organisms are subjected to contrasting fitness effects and may evolve to manipulate host mating behavior in a sex-specific manner: decreasing male mating to avoid cannibalism and promoting female mating to engage in cannibalism. We examined this hypothesis by a behavioral experiment using a mantid-hairworm system. Parasitized male mantids (Tenodera angustipennis) changed their behavior as expected, exhibiting increased escapes and decreased courtships and mountings, potentially avoiding encounters with the female. Interestingly, male attack behavior was promoted, possibly decreasing contact with the encountered female. However, contrary to our prediction, parasitized females also exhibited decreased propensities of mating, suggesting costs or little benefits of host mating for parasites in the female host. This study provides novel insights into the evolution of parasite strategies in response to sexual differences in host quality.Oxford University Press (OUP), 2024年09月, Behavioral Ecology, 35, arae071, 英語[査読有り]研究論文（学術雑誌）
- Springer Science and Business Media LLC, 2024年07月, Behavioral Ecology and Sociobiology, 78, 82, 英語[査読有り]研究論文（学術雑誌）
- Abstract In reproductive interference (RI), the fitness of individuals or populations is decreased through reproductive interactions with other species. This process results in positive frequency dependence, hindering species coexistence. However, theory predicts that species can coexist under weak RI. Habitat segregation can decrease the opportunity for reproductive interactions between species. Thus, a difference in habitat preference between species may weaken RI and facilitate coexistence. We examined this hypothesis by investigating the habitat uses of closely related Ohomopterus ground beetle species under RI, Carabus insulicola and C. esakii, in limited zones of sympatry at their distributional boundary. The effect of RI may be stronger for females of C. esakii than those of C. insulicola due to asymmetry in the genital size mismatch between the species (C. insulicola males have larger genitalia). Field surveys of local abundances and associations with local environmental parameters revealed contrasting habitat uses between the species. C. insulicola preferred open environments, while C. esakii inhabited forest environments. Interestingly, the habitat use of C. esakii, not C. insulicola, changed depending on the frequency of the other species; the species utilized habitats with a low frequency of C. insulicola. The difference in habitat use and its dependence on the frequency of the other species may facilitate species coexistence by promoting habitat segregation within a continuous landscape. Our findings provide insights into the importance of (plastic) trait differences in species distributions and coexistence under RI.Wiley, 2024年07月, Population Ecology, 67, 6 - 14, 英語[査読有り]研究論文（学術雑誌）
- Abstract Extensive research in evolutionary biology has focused on the exaggeration of sexual traits; however, the developmental basis of exaggerated sexual traits has only been determined in a few cases. The evolution of exaggerated sexual traits may involve the relaxation of constraints or developmental processes mitigating constraints. Ground beetles in the subgenus Ohomopterus (genus Carabus) have species‐specific genitalia that show coevolutionary divergence between the sexes. Here, we examined the morphogenesis of the remarkably enlarged male and female genitalia of Carabus uenoi by X‐ray microcomputed tomography. The morphogenetic processes generating the male and female genitalia at the pupal stage were qualitatively similar to those in closely related species with standard genital sizes. Higher growth rates contributed to the exaggeration of both the male and female genital parts of C. uenoi, possibly related to a gene network commonly upregulated in both sexes. Additionally, the length of the copulatory piece (CP), the enlarged male genital part stored in the aedeagus (AD), reached close to that of the AD at the later developmental stages and thereafter decelerated to grow in parallel with the AD, suggesting a structural constraint on the CP by the outer AD. Then, unlike related species, the lengths of the CP and AD increased at eclosion, suggesting a mechanism leading to further elongation of the male genitalia. These observations suggest that a developmental process allows continuous growth of the male genitalia even under the spatial limitation. These results revealed the spatio‐temporal dynamics of the development of exaggerated genital structures under structural constraints.Wiley, 2024年06月, Evolution & Development, 26, e12488, 英語[査読有り]研究論文（学術雑誌）
- Wiley, 2023年01月, Entomological Science, 26(1) (1), e12538[査読有り]研究論文（学術雑誌）
- Abstract Natural selection against maladaptive interspecific reproductive interactions may cause greater divergence in mating traits between sympatric populations than between allopatric populations in a pair of species, known as reproductive character displacement (RCD) which is evidence for the lock-and-key hypothesis of genital evolution. However, the relative importance of various processes contributing to RCD in genital morphology (e.g. reinforcement, reproductive interference, and population filtering or the Templeton effect) is not clear. Here, we examined hypotheses for RCD in genital morphology, with a special focus on the Templeton effect (which predicts that only highly differentiated populations can exist in sympatry). We examined population-level fitness costs in interspecific mating between Carabus maiyasanus and Carabus iwawakianus with RCD in genital morphology. A mating experiment using populations with various degrees of RCD in genital morphology showed no evidence for consistently lower interspecific mating costs in C. maiyasanus populations in contact with displacement in genital morphology than in remote populations, contrary to the predictions of the Templeton effect. Alternatively, interspecific mating costs varied among populations. Observed relationships between the sizes of genital parts concerning isolation and interspecific mating costs across populations suggested that population-level fitness costs do not necessarily decrease during the process leading to RCD. Our results provide insight into ecological and evolutionary processes during secondary contact in closely related species.Oxford University Press (OUP), 2023年01月, Biological Journal of the Linnean Society, 138, 14 - 26, 英語[査読有り]研究論文（学術雑誌）
- Wiley, 2023年01月, Journal of Evolutionary Biology, 36, 169 - 182[査読有り]研究論文（学術雑誌）
- 2022年12月, Proceedings of the Arthropodan Embryological Society of Japan, 54, 1 - 9, 英語Paranotal lobes are appendicular in origin: elucidation by micro-CT analysis of the thoracic muscular system in the larvae of Carabus insulicola (Insecta, Coleoptera)[査読有り]研究論文（学術雑誌）
- The Royal Society, 2022年03月, Biology Letters, 18(3) (3), 20220010, 英語[査読有り][招待有り]研究論文（学術雑誌）
- Wiley, 2022年01月, Ecological Entomology, 47, 423 - 431, 英語[査読有り]研究論文（学術雑誌）
- Spatial and temporal variation in ecological environments may result in spatial and temporal variation in communities. Temporal studies of biodiversity are essential for forecasting future changes in community structure and ecosystem function. Therefore, determining the mechanisms that drive temporal change in communities remains an important and interesting challenge in ecology. We quantified spatial and temporal variations in carabid beetle communities and site-specific environmental factors for 5 years at nine study sites on three mountains in the Baekdudaegan Mountain Range, Korea. Carabid beetle communities exhibited significant temporal variation, which was larger than spatial variations between and within mountains. Environmental factors mostly varied between sites within mountains. Community variation was only weakly associated with environmental factors at wide scales, i.e., between sites on three mountains, but was strongly associated at narrow spatial scales, i.e., between sites within one mountain. Our results indicate that temporal variation in communities occurs in response to variations in the local climate, and that the patterns of temporal variation differ between mountains. Thus, temporal surveys of insect communities and climates at local scales are important for predicting temporal changes in the communities.MDPI AG, 2021年11月, Insects, 12(11) (11), 1019 - 1019, 英語[査読有り]研究論文（学術雑誌）
- The origin and diversification process of lineages of organisms that are currently widely distributed among continents is an interesting subject for exploring the evolutionary history of global species diversity. Ground beetles of the subfamily Carabinae are flightless except for one lineage, but nevertheless occur on all continents except Antarctica. Here, we used sequence data from ultraconserved elements to reconstruct the phylogeny, divergence time, biogeographical history, ancestral state of hind wings and changes in the speciation rate of Carabinae. Our results show that Carabinae originated in the Americas and diversified into four tribes during the period from the late Jurassic to the late Cretaceous, with two in South America (Celoglossini) and Australasia (Pamborini) and two in Laurasia (Cychrini and Carabini). The ancestral Carabinae were inferred to be winged; three of four tribes (Cychrini, Ceglossini and Pamborini) have completely lost their hind wings and flight capability. The remaining tribe, Carabini, diverged into the subtribes Carabina (wingless) and Calosomina (winged) in the Oligocene. Carabina originated in Europe, spread over Eurasia and diversified into approximately 1000 species, accounting for around 60% of all Carabinae species. Calosomina that were flight-capable dispersed from North America or Eurasia to South America, Australia, and Africa, and then flightless lineages evolved on oceanic islands and continental highlands. The speciation rate increased in the Cychrini and Carabini clades in Eurasia. Within Carabini, the speciation rate was higher for wingless than winged states. Our study showed that the global distribution of Carabinae resulted from ancient dispersal before the breakup of Gondwana and more recent dispersal through flight around the world. These patterns consequently illustrate the causal relationships of geographical history, evolution of flightlessness, and the global distribution and species diversity of Carabinae.Elsevier BV, 2021年11月, Molecular Phylogenetics and Evolution, 167, 107355 - 107355, 英語[査読有り]研究論文（学術雑誌）
- University of Chicago Press, 2021年10月, The American Naturalist, 199(3) (3), E76 - E90, 英語[査読有り]研究論文（学術雑誌）
- Oxford University Press (OUP), 2021年10月, Biological Journal of the Linnean Society, 135, 184 - 194
Abstract The prey preference of a predator can impose natural selection on prey phenotypes, including body size. Despite evidence that large body size protects against predation in insects, the determinants of body size variation in Cassidinae leaf beetles are not well understood. We examined the prey preference of the digger wasp Cerceris albofasciata, a specialist predator of adult Cassidinae leaf beetles, and found evidence for natural selection on prey body size. The wasp hunted prey smaller than the size of their nest entrance. However, the wasp preferred larger prey species among those that could be carried into their nest. Thus, the benefits of large prey and the cost associated with nest expansion might determine the prey size preference. As expected from the prey species preference, the wasp preferred small individuals of the largest prey species, Thlaspida biramosa, and large individuals of the smallest prey species, Cassida piperata, resulting in natural selection on body sizes. In intermediate-sized prey species, however, there was no evidence for selection on body size. Natural selection on body size might explain the variation of prey morphologies that increase body size, such as explanate margins, in this group.[査読有り]研究論文（学術雑誌） - The Indian subcontinent has an origin geologically different from Eurasia, but many terrestrial animal and plant species on it have congeneric or sister species in other parts of Asia, especially in the Southeast. This faunal and floral similarity between India and Southeast Asia is explained by either of the two biogeographic scenarios, ‘into-India’ or ‘out-of-India’. Phylogenies based on complete mitochondrial genomes and five nuclear genes were undertaken for ricefishes (Adrianichthyidae) to examine which of these two biogeographic scenarios fits better. We found thatThe Royal Society, 2021年08月, Biology Letters, 17(8) (8), 20210212 - 20210212, 英語, 国際誌
Oryzias setnai , the only adrianichthyid distributed in and endemic to the Western Ghats, a mountain range running parallel to the western coast of the Indian subcontinent, is sister to all other adrianichthyids from eastern India and Southeast–East Asia. Divergence time estimates and ancestral area reconstructions reveal that this western Indian species diverged in the late Mesozoic during the northward drift of the Indian subcontinent. These findings indicate that adrianichthyids dispersed eastward ‘out-of-India’ after the collision of the Indian subcontinent with Eurasia, and subsequently diversified in Southeast–East Asia. A review of geographic distributions of ‘out-of-India’ taxa reveals that they may have largely fuelled or modified the biodiversity of Eurasia.[査読有り]研究論文（学術雑誌） - Wiley, 2021年04月, Entomological Science, 24(3) (3), 219 - 227[査読有り]研究論文（学術雑誌）
- The rapid divergence of genital morphology is well studied in the context of sexual selection and speciation; however, little is known about the developmental mechanisms underlying divergence in genitalia. Ground beetles in the subgenus Ohomopterus genus Carabus have species-specific genitalia that show coevolutionary divergence between the sexes. In this study, using X-ray microcomputed tomography, we examined the morphogenesis of male and female genitalia in two closely related Ohomopterus species with divergent genital morphologies. The morphogenetic processes generating the male and female genitalia at the pupal stage were qualitatively similar in the two species. The male aedeagus and internal sac and female bursa copulatrix were partially formed at pupation and developed gradually thereafter. The species-specific genital parts, male copulatory piece, and female vaginal appendix differed in the timing and rate of development. The relatively long copulatory piece of Carabus maiyasanus began to develop earlier, but subsequent rates of growth were similar in the two species. The timing of the formation of the vaginal appendix and initial growth rates were similar, but subsequent rapid growth led to a longer vaginal appendix in C. maiyasanus. Thus, substantial interspecific differences in the size of genital parts were mediated by different underlying developmental mechanisms between the sexes (i.e., a shift in the developmental schedule in males and a change in growth rate in females). These results revealed the spatio-temporal dynamics of species-specific genital structure development, providing a novel platform for evo-devo studies of the diversification of genital morphologies.Wiley, 2021年01月, Evolution & Development, 23(1) (1), 19 - 27, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Oxford University Press (OUP), 2020年12月, Biological Journal of the Linnean Society, 131(4) (4), 936 - 951, 英語
Abstract Ecological character displacement predicts that interspecific resource competition results in greater trait divergence between species in sympatry than in allopatry. However, other processes, such as ecological sorting, result in the same pattern of trait variation. In this study, we characterize character displacement in eight species of snail-feeding Acoptolabrus ground beetles in the Far East. Acoptolabrus exhibit divergent feeding phenotypes, including species with a slender forebody that is able to intrude into large shells and species with stout heads and mandibles for crushing small shells. The pattern of character displacement in feeding phenotypes was confirmed by multivariate analysis of body dimensions. Molecular phylogenetic analysis, divergence time estimation and biogeographical analysis revealed that sympatry and phenotypic divergence occurred repeatedly during the Pleistocene and almost simultaneously within each geographical area. Comparative analysis revealed that the evolution of feeding phenotypes best fitted a selective model with three adaptive optima, corresponding to the three cases of sympatry with a congener. Repeated coincidences of sympatry and adaptive differentiation in feeding phenotypes suggested causal relationships, although the precise order of events was difficult to discriminate. This study provides insight into the spatiotemporal dynamics of interspecific interactions and adaptive phenotypic diversification.[査読有り]研究論文（学術雑誌） - Oxford University Press (OUP), 2020年08月, Biological Journal of the Linnean Society, 131(1) (1), 50 - 60, 英語
Abstract Some highly isolated oceanic islands harbour endemic ground beetles that have lost the ability to fly. Here, we investigated the origin of the possibly extinct flightless giant ground beetle Aplothorax burchelli on St Helena Island in the South Atlantic. Aplothorax burchelli was initially considered to be a member of the subtribe Calosomina (=genus Calosoma) of the subfamily Carabinae (Coleoptera: Carabidae) closely related to the genus Ctenosta (=Calosoma subgenus Ctenosta), but this proposition was questioned due to its unique external and genital morphology. We conducted a phylogenetic analysis of mitogenome sequences using historical specimens of A. burchelli and samples of representative species of Carabinae. Our analysis of 13 protein-coding gene sequences revealed that A. burchelli is definitely a member of Calosomina, most closely related to a species of Ctenosta. Further analysis using NADH dehydrogenase subunit 5 gene sequences from most groups in Calosomina showed that A. burchelli formed a monophyletic group with Ctenosta species from Africa and Madagascar. Our results suggest that the ancestor of A. burchelli, which had the ability to fly, colonized St Helena from Africa after the emergence of the island 14 Mya, and has since undergone evolutionary changes in conjunction with loss of flight.[査読有り]研究論文（学術雑誌） - 2020年, Ecology and Evolution, 10, 5089 - 5096, 英語[査読有り]研究論文（学術雑誌）
- Wiley, 2019年04月, Ecosphere, 10(4) (4), e02628, 英語[査読有り]研究論文（学術雑誌）
- Sexual traits are subject to evolutionary forces that maximize reproductive benefits and minimize survival costs, both of which can depend on environmental conditions. Latitude explains substantial variation in environmental conditions. However, little is known about the relationship between sexual trait variation and latitude, although body size often correlates with latitude. We examined latitudinal variation in male and female sexual traits in 22 populations of the false blister beetle Oedemera sexualis in the Japanese Archipelago. Males possess massive hind legs that function as a female-grasping apparatus, while females possess slender hind legs that are used to dislodge mounting males. Morphometric analyses revealed that male and female body size (elytron length), length and width of the hind femur and tibia, and allometric slopes of these four hind leg dimensions differed significantly among populations. Of these, three traits showed latitudinal variation, namely, male hind femur was stouter; female hind tibia was slenderer, and female body was smaller at lower latitudes than at higher latitudes. Hind leg sizes and shapes, as measured by principal component analysis of these four hind leg dimensions in each sex, covaried significantly between sexes, suggesting coevolutionary diversification in sexual traits. Covariation between sexes was weaker when variation in these traits with latitude was removed. These results suggest that coevolutionary diversification between male and female sexual traits is mediated by environmental conditions that vary with latitude.2019年, Ecology and Evolution, 9(8) (8), 4949 - 4957, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- The diversity of genital morphology among closely related animals with internal fertilization is well known, but the genetic backgrounds are unclear. Here, we show that, in Carabus (Ohomopterus) beetles showing correlated evolution of male and female genital parts, only a few major quantitative trait loci (QTLs) determine differences in genital dimensions between sister species, and sequence divergence is pronounced in the genomic regions containing genital QTLs. The major QTLs for male and female genital dimensions reside in different locations within the same linkage group, implying that coevolution between the sexes is only loosely constrained and can respond to sexually antagonistic selection. The same genomic regions containing the major QTLs show elevated divergence between three pairs of parapatric species with marked differences in genital parts. Our study demonstrates that species diversification can follow coevolution of genitalia between the sexes, even without tight linkage of loci affecting male and female genital dimensions.2019年, Science Advances, 5(6) (6), eaav9939, 英語, 国際誌[査読有り]研究論文（学術雑誌）
- Springer Science and Business Media {LLC}, 2018年05月, Journal of Ethology, 36(2) (2), 211 - 211研究論文（学術雑誌）
- Wiley, 2018年, Evolution, 72(11) (11), 2449 - 2461, 英語[査読有り]研究論文（学術雑誌）
- While competing males and choosy females may be common in animal mating systems, male choice can evolve under certain conditions. Sexual cannibalism is such a condition because of the high mortality risk for males. In mantids, female body condition is associated with male mate preference, with fat females preferred, due to at least two reasons: females in poor nutritional condition are likely to attack and predate males, and fat females can potentially increase the number of offspring. Thus, the risk of cannibalism and female fecundity can influence male mating behavior. In this study, we attempted to separate these factors by using the praying mantid Tenodera angustipennis to examine whether male preference for fat female mantids was based on avoiding sexual cannibalism (cannibalism avoidance hypothesis) or preference for female fecundity (fecundity preference hypothesis). The feeding regimes were experimentally manipulated to discriminate between the effects of female fecundity and female hunger status on male and female mating behaviors. We found that recently starved females more frequently locomoted toward the male, and that male abdominal bending was less intensive and escape was sooner from recently starved females. These female and male behavioral responses to female hunger condition may reveal male avoidance of dangerous females in this mantid.SPRINGER JAPAN KK, 2017年05月, Journal of Ethology, 35(2) (2), 177 - 185, 英語[査読有り]研究論文（学術雑誌）
- 1. Global environmental change can dramatically alter the composition of floral and faunal communities, and elucidating the mechanisms underlying this process is important for predicting its outcomes. Studies on global climate change have mostly focused on statistical summaries within wide spatial and temporal scales; less attention has been paid to variability in microclimates at narrower spatial and temporal scales. 2. The microclimate is the suite of climatic conditions measured in a local area. Environmental variables at the microclimatic scale can be critical for the ecology of organisms inhabiting each area. The effect of spatial and temporal changes in the microclimate on the ecology of carabid beetle communities in three sites on Mount Odaesan, Korea was examined. 3. Carabid beetle communities and quantified site-specific environmental factors from measurements of air temperature, air humidity, light intensity and soil temperature over 5 years (2010-2015) were surveyed. 4. It was found that microclimatic variables and the patterns of temporal changes in carabid beetle communities differed between the three sites within the single mountain system. Microclimatic variables influencing temporal changes in beetle communities also differed between the sites. Therefore, it is suggested that variation in local microclimates affects spatial and temporal variation in carabid beetle communities at a local scale. 5. The present results demonstrate the importance of regular surveys of communities at local scales. Such surveys are expected to reveal an additional fraction of variation in communities and underlying processes that have been overlooked in studies of global community patterns and change.WILEY, 2017年04月, ECOLOGICAL ENTOMOLOGY, 42(2) (2), 184 - 194, 英語[査読有り]研究論文（学術雑誌）
- 2017年, Entomologische Blätter und Coleoptera, 113(1) (1), 213 - 218, 英語Adult leaf beetles of the subfamily Cassidinae (Coleoptera: Chrysomelidae) preyed on by the digger wasp Cerceris albofasciata (Hymenoptera: Crabronidae) in Japan[査読有り]研究論文（学術雑誌）
- Celes akitanus was rediscovered in semi-natural grasslands in Japan. This species is one of the most endangered insects in Japan; most local populations were thought to be extinct. Reliable records of this rare species had been obtained only from Yamagata, Niigata, Tokyo and Nagano Prefectures. We compared the morphology of the holotype of Oedipoda akitana (= C. akitanus) and newly collected specimens and found that they matched with each other. The species was redescribed based on the holotype and new specimens. A molecular phylogenetic analysis involving other acridid species indicated that our samples were practically identical to C. akitanus from China. We conclude that the specimens we collected are C. akitanus, which has been rediscovered in Japan after about 30years. Because the discovered populations were restricted to narrow and scattered areas, conservation of these populations should be a high priority. Population monitoring and habitat assessment are required. Because C. akitanus and many other endangered species are found in the grassland areas, these habitats should be protected and maintained.WILEY-BLACKWELL, 2016年04月, ENTOMOLOGICAL SCIENCE, 19(2) (2), 89 - 96, 英語[査読有り]研究論文（学術雑誌）
- Exogenous selection via interactions between organisms and environments may influence the dynamics of hybrid zones between species in multiple ways. Two major models of a hybrid zone allowed us to hypothesize that environmental conditions influence hybrid zone dynamics in two ways. In the first model, an environmental gradient determines the mosaic distribution at the boundary between ecologically differentiated species (mosaic hybrid zone model). In the second model, a patch of unsuitable habitat traps a hybrid zone between species whose hybrids are unfit (tension zone model). To test these, we examined the environmental factors influencing the spatial structure of a hybrid zone between the ground beetles Carabus maiyasanus and C. iwawakianus using GIS-based quantification of environmental factors and a statistical comparison of species distribution models (SDMs). We determined that both of the hypothetical processes can be important in the hybrid zone. We detected interspecific differences in the environmental factors in presence localities and their relative contribution in SDMs. SDMs were not identical between species even within contact areas, but tended to be similar within the range of each species. These results suggest an association between environments and species, and provide evidence that ecological differentiation between species plays a role in the maintenance of the hybrid zone. Contact areas were characterized by a relatively high temperature, low precipitation, and high topological wetness. Thus, the contact areas were regarded as being located in an unsuitable habitat with a drier climate, where those populations are likely to occur in patches with limited precipitation concentrated. A comparison of spatial scales suggests that exogenous selection via environmental factors may be weaker than endogenous selection via genitalic incompatibility.WILEY-BLACKWELL, 2016年01月, ECOLOGY AND EVOLUTION, 6(1) (1), 113 - 124, 英語[査読有り]研究論文（学術雑誌）
- 日本教育大学協会第二常置委員会, 2015年03月, 日本教育大学協会研究年報, 33, 87 - 99, 日本語学内博物館実習を活用したサービスラーニングの試みと効果[査読有り]研究論文（学術雑誌）
- Although apomixis is the most common form of parthenogenesis in diplodiploid arthropods, it is uncommon in the haplodiploid insect order Hymenoptera. We found a new type of spontaneous apomixis in the Hymenoptera, completely lacking meiosis and the expulsion of polar bodies in egg maturation division, on the thelytokous strain of a parasitoid wasp Meteorus pulchricornis (Wesmael) (Braconidae, Euphorinae) on pest lepidopteran larvae Spodoptera litura (Fabricius) (Noctuidae). The absence of the meiotic process was consistent with a non-segregation pattern in the offspring of heterozygous females, and no positive evidence was obtained for the induction of thelytoky by any bacterial symbionts. We discuss the conditions that enable the occurrence of such rare cases of apomictic thelytoky in the Hymenoptera, suggesting the significance of fixed heterosis caused by hybridization or polyploidization, symbiosis with bacterial agents, and occasional sex. Our finding will encourage further genetic studies on parasitoid wasps to use asexual lines more wisely for biological control.CAMBRIDGE UNIV PRESS, 2014年06月, BULLETIN OF ENTOMOLOGICAL RESEARCH, 104(3) (3), 307 - 313, 英語[査読有り]研究論文（学術雑誌）
- WILEY-BLACKWELL, 2014年03月, PHYSIOLOGICAL ENTOMOLOGY, 39(1) (1), 12 - 18, 英語Inhibition of female mating by male accessory gland substances in the ground beetle Leptocarabus procerulus[査読有り]研究論文（学術雑誌）
- 生物地理学は、歴史的側面や生態的側面などの観点から、生物の分布パターンや分布形成プロセスの解明を目指す学問であり、進化生態学や群集生態学、保全生物学などの分野とも強い関連をもつ学際的領域である。1990年代以降、遺伝解析技術の恩恵を受けた系統地理学の隆盛によって、生物地理学は大きな発展を遂げてきた。さらに近年では「地理情報システム(GIS)」や、それを利用した「気候シミュレーション」、「生態ニッチモデリング」といった新たな解析ツールが、生物地理学分野に新しい流れを生み出しつつある。その基礎的な活用例として、現在の生物種の分布情報と気候要因から生態ニッチモデルを構築し、気候シミュレーションから得られた異なる時代の気候レイヤに投影するというアプローチが挙げられる。これにより、過去や現在、未来における生物の分布を予測することが可能となり、時間的な分布変化を推定することができる。さらに、GISを活用して、モデル化された生態ニッチや系統地理学的データを複合的に解析することで、近縁種間でのニッチ分化や、分布変遷史を考慮に入れた種分化要因の検証、群集レベルでの分布変遷史の検証なども可能となる。本総説では、最初に基礎的な解析ツールについて解説した後、実際にこれらのツールを活用した生物地理学とその関連分野における研究例を紹介する。最後に、次世代シークエンシングによって得られる膨大な遺伝情報や古DNAデータの有用性について紹介した後、それらの情報を用いた生物地理学や関連分野における今後の展望について議論し、GIS技術がその中で重要な役割を果たしうることを示す。日本生態学会暫定事務局, 2014年, 日本生態学会誌, 64(3) (3), 183 - 199, 日本語[査読有り]
- The egg morphology and successive changes in the developing embryos of the carabid ground beetle Carabus insulicola (Carabidae) are described based on light and scanning electron microscopy observations. Newly laid eggs of this species are ellipsoid and measure approximately 6.1 x 2.9 mm, before increasing to 6.6 x 3.4 mm at hatching. The egg period is about 11 days at 23 degrees C. The egg shell is characterized by a thin fragile chorion covering a hard serosal cuticle. The embryo forms on the ventral egg surface, where it develops for the duration of the egg period. During the process of thoracic leg formation, two subcoxal rings, subcoxae-1 and 2, are clearly discernible at the basalmost region of the leg rudiments, and these subcoxae participate in the formation of the larval pleura and sterna. The result thus provides tangible evidence for the subcoxal theory, that is, that thoracic pleura and sterna are derived from subcoxal regions. Despite the complete absence of abdominal appendages in the larvae of this species, two pairs of appendage-like swellings, the medial and lateral ones, temporarily arise in the first eight abdominal segments during the middle of embryonic development. The medial swellings are assumed to be serially homologous with the coxal part of the thoracic leg, and they later flatten out and participate in the formation of the larval pleura (hypopleurites). In the light of the serially homologous relationships among gnathal appendages, thoracic legs, and abdominal appendage-like swellings, we identified the subcoxal regions in both the gnathal and abdominal segments. Although, the lateral swellings soon degenerate and disappear, it is considered that the swellings originate in the abdominal subcoxae-2 and may be homologous to the tracheal gills of larvae of Gyrinidae. Based on the embryological results, new interpretations for the constituent of gnathal appendages are proposed. J. Morphol. 274:1323-1352, 2013. (c) 2013 Wiley Periodicals, Inc.WILEY-BLACKWELL, 2013年12月, JOURNAL OF MORPHOLOGY, 274(12) (12), 1323 - 1352, 英語[査読有り]研究論文（学術雑誌）
- The decrease in biodiversity due to increasing urbanization has been well documented, but the processes of colonization and maintenance of wildlife populations in urban areas remain poorly understood. We address this issue using 462 individuals from 10 urban populations of the ant Formica japonica in Kobe City, Japan. We sampled workers regardless of colony identity, genotyped them using 6 microsatellite loci, and estimated allele frequencies and genotypes of reproductive individuals, together with other population genetic parameters, by estimating kinship structure using a likelihood method. Estimated genetic diversity and effective size of populations were not associated with environmental parameters, suggesting that populations are unaffected by urbanization. However, effective population sizes were small, and frequent population bottlenecks were detected. These results suggest that urban F. japonica populations are unstable, and the possibility of frequent extinctions and recolonizations in urban habitats. Populations were moderately differentiated without isolation by distance, suggesting a strong dispersal ability that enables colonization of urban habitats. Dispersal was male biased. Collectively, F. japonica was regarded as an urban adapter, which can colonize urban habitats by virtue of its preference for open ground and high dispersal ability but can persist in urban populations for only a short time, showing a tendency as a temporary urban inhabitant.OXFORD UNIV PRESS INC, 2013年09月, JOURNAL OF HEREDITY, 104(5) (5), 639 - 648, 英語[査読有り]研究論文（学術雑誌）
- The mechanical isolation hypothesis predicts that physical incompatibility between divergent reproductive morphologies hinders hybridization between populations. However, evidence for this hypothesis remains scarce. We examined this hypothesis using two parapatric carabid beetles, Carabus insulicola and C. esakii, which are of the subgenus Ohomopterus and exhibit a species-specific genital lock-and-key system. Our interspecific crossing experiment revealed that incompatibility of genital morphologies served as a strong postmating-prezygotic isolation barrier. This isolation was asymmetric: a decrease in female fitness was more costly in the cross with greater genitalic incompatibility between a C. esakii female and a C. insulicola male. These two species share a limited sympatric area, but the mechanism responsible for their coexistence is unclear given no evidence of premating isolation via male mate choice. A comparison of the present results with those of previous studies that quantified reproductive isolation between Ohomopterus species suggest that strong mechanical isolation via genitalic incompatibility plays a major role in species isolation, but that it may be less important in species coexistence.SPRINGER JAPAN KK, 2013年01月, POPULATION ECOLOGY, 55(1) (1), 35 - 42, 英語[査読有り]研究論文（学術雑誌）
- The body size of a univoltine carabid beetle Carabus tosanus on Shikoku Island, Japan, was clearly smaller in higher-altitude populations (subspecies), which possibly represents incipient speciation. To explore the determinants of altitudinal differences in body size in this species, we studied the degree of phenotypic plasticity by conducting rearing experiments at two constant temperatures and examined genetic differences through interpopulation crosses. At 15 degrees C, C. tosanus had a longer developmental period and a shorter adult body than at 20 degrees C. Nevertheless, variation in body size due to temperature effects (phenotypic plasticity) was small compared to the interpopulation differences, which suggests substantial genetic differences between populations (subspecies) at different altitudes. In F1 offspring from crosses between a low-altitude (subspecies tosanus) and a high-altitude population (subspecies ishizuchianus), adult body length was affected by the genotypes of both parents, with an interaction effect of parental genotype and offspring sex. Further analyses revealed that adult body length was affected by sex-linked factors in addition to autosomal factors. These genetic differences in body size may have resulted from adaptations to different altitudes and may be important for the process of incipient speciation because body size differences could contribute to premating reproductive isolation.WILEY-BLACKWELL, 2012年09月, JOURNAL OF EVOLUTIONARY BIOLOGY, 25(9) (9), 1835 - 1842, 英語[査読有り]研究論文（学術雑誌）
- The morphologies of male genitalia often appear harmful or aggressive, as if they may inflict physical damage upon females during copulation. Such male genitalia are often thought to function in intra-and intersexual interactions during mating. In the carabid genus Carabus, division Spinulati, males possess a spine (spinula) on the intromittent organ, of which function is unknown. To reveal the function of the spinula, we studied the mating behavior and genital coupling of a Spinulati species, Carabus (Limnocarabus) clathratus. The males positioned the spinula along the inner wall of the vaginal opening throughout copulation. This placement created a small dent and subsequently a melanized patch (wound) on the vaginal wall, but the spinula rarely penetrated the vaginal wall. The spinula did not reach the innermost part of the vagina where the spermatophore is deposited. These results suggest that the spinula is not used for inflicting damage on female genitalia or manipulating spermatophores of rival males. During spermatophore formation, the male partially withdrew the aedeagus, and only the aedeagal tip and endophallus remained within the female. By placing the spinula against the vaginal wall, the male could hold the endophallus within the vaginal chamber in the unstable copulatory posture. Thus, our observations suggest that the spinula primarily functions as an "anchor" to maintain the coupling of the male and female genitalia and thereby ensure insemination.ZOOLOGICAL SOC JAPAN, 2012年07月, ZOOLOGICAL SCIENCE, 29(7) (7), 428 - 432, 英語[査読有り]研究論文（学術雑誌）
- 生態的に似た複数の近縁種が共存するには、種間の資源競争あるいは繁殖干渉が緩和される必要がある。オサムシ科オオオサムシ亜属は、成虫期に多食性の捕食者であるが、幼虫期はミミズ専食である。またオスは異種のメスに対しても交尾行動を示す。彼らは分布域の大部分で2-3種が共存しており、同所的に分布する種間では体サイズが異なっている。この種間の体サイズ差は、幼虫期に捕食可能なミミズのサイズに応じた資源分割をもたらし、資源競争を緩和するかもしれない。また成虫期に異種間の交尾行動を機械的に妨げる生殖隔離として、繁殖干渉を緩和するかもしれない。この2つの仮説を、京都に分布するオオオサムシ亜属4種(山間部の大、中、小型の3種、平野部の大型1種)を用いて検証した。まず、4種の幼虫(1-3齢)に様々なサイズのミミズを与えた。その結果、すべての幼虫は、ミミズのサイズに関わらず捕食行動を示した。またミミズのサイズ増加に伴う捕食失敗は、小型種の1齢幼虫期でのみ観察された。したがって、種間の体サイズ差は資源分割に有効ではないと考えられた。次に、4種の成虫で16通りの雌雄ペアを作り、交尾行動(交尾意欲、マウント、交尾器の挿入、精包形成)を観察した。その結果、体サイズ差が大きい異種ペアでは、交尾意欲があっても交尾器が届かず、挿入ができないペアが多かった。すなわち、種間の体サイズ差による交尾前生殖隔離が成立しており、このことが体サイズ差の大きい近縁種の同所的分布を可能にしていると考えられた。一方、体サイズ差が小さい異種ペア(山間部の大、中型種と平野部の大型種のペア)では、大半のペアで交尾器の挿入が行われ、精包形成まで達成するペアも見られた。このことから、体サイズの似た種が同所的に分布しないのは、資源競争ではなく繁殖干渉のためであることが示唆された。日本生態学会暫定事務局, 2012年, 日本生態学会誌, 62: 275-285(2) (2), 275 - 285, 日本語[査読有り]
- {SAGE} Publications, 2011年, International Journal of Insect Science, 3: 1-10, 英語[査読有り]研究論文（学術雑誌）
- Animals with internal fertilization often exhibit marked diversification in genital morphology among closely related species. However, our knowledge of the genetic architecture underlying genital evolution is still limited. We constructed genetic linkage maps and analysed quantitative trait loci (QTL) for F(2) hybrids of two closely related species of the carabid beetles Carabus (Ohomopterus) iwawakianus and C. (O.) maiyasanus, which show matching male and female genital shapes within species, but marked differences in genital morphologies between species. The linkage maps comprised both amplified fragment length polymorphism and microsatellite markers. Composite interval mapping to detect QTL for three traits of male copulatory piece (length, width, weight) and two traits for female vaginal appendix (length, width) resulted in the detection of one to five significant QTL for each trait. The QTL explained large proportions of phenotypic variance. Thus, the interspecific difference in the genital morphologies appeared to be determined by relatively small numbers of genes with large genetic effects. QTL of different traits for the same or different sexes co-occurred on five of eight linkage groups with significant QTL; in particular, three QTL for different male and female genital traits occurred almost at the same position. Each of the male genital traits showed uniform signs of additive genetic effects, suggesting that directional selection has led to species-specific morphologies. However, the signs of additive genetic effects in each female genital trait were not uniform, suggesting that coevolution between sexes is not necessarily concerted. This result requires further assessment because the sample size of F(2) females was small.WILEY-BLACKWELL PUBLISHING, INC, 2010年12月, MOLECULAR ECOLOGY, 19(23) (23), 5231 - 5239, 英語[査読有り]研究論文（学術雑誌）
- The Pompilidae (spider hunting wasps) show a marked diversity in their hunting and reproductive ecology, but difficulties in phylogenetic taxonomy have hindered the elucidation of their evolutionary processes. We present here a review of the hunting and reproductive ecology of the pepsine tribe Ageniellini (including the first prey record for Macrornerella), and phylogenetic analyses of this group to reconstruct the evolution of nestconstructing behaviour. The maximum parsimony and Bayesian inference analyses are based on 45 adult morphological characters, coded for 41 exemplar species, including five out-group species, representing almost all the genera and subgenera in this tribe. Results supported the following eight clades uniting more than one genus: (1) cyphononyx + Cryptocheilus + Agernelhni; (2) Agenielhni; (3) Auplopodina, containing Dirnorphagenia, Auplopus, Machaerothrix, Phanagenia, Dichragenia, Paragenia, Macromerella, and Macrorneris; (4) Phanagenia + Dichragenia + Auplopus artemis + Paragenia + Macromerella + Macromeris; (5) Auplopus arternis + Paragenia + Macromerella + Macromeris; (6) Paragenia + Macromerella + Macrorneris; (7) Macrornerella + Macromeris; and (8) Agenidlla (Lissageiia) + Pitaitochilac. The monophyly of the genera Aaplopus and Ageniella s.l. was not confirmed. The evolution of nesting behaviour was inferred, as burrowing in the soil without using water was ancestral in Ageniellini, and constructing nests by plastering mud obtained by softening soil with water was derived only once at the ancestor of the Auplopodina. The origin of cleptoparasitism was unclear. The origin of communal nesting is also discussed in relation to the evolution of nestconstructing behaviour. The following new combinations are proposed: Ageniella (Alasagenia) sartoriana (Cresson) comb. nov., C'yemagenia certator (Nurse) comb. nov., and Auplopus artemis (Bingham) comb, nov. (C) 2010 The Linnean Society of London, Zoological Journal of the Linnean Society, 2010, 160, 88-117.WILEY-BLACKWELL, 2010年09月, ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, 160(1) (1), 88 - 117, 英語[査読有り]研究論文（学術雑誌）
- 2010年, Journal of Animal Ecololgy, 78: 383-392, 383 - 392, 英語Resource partitioning or reproductive isolation: The ecological role of body size differences among closely related species in sympatry[査読有り]研究論文（学術雑誌）
- Zygomorphic flowers are usually more complex than actinomorphic flowers and are more likely to be visited by specialized pollinators. Complex zygomorphic flowers tend to be oriented horizontally. It is hypothesized that a horizontal flower orientation ensures effective pollen transfer by facilitating pollinator recognition (the recognition-facilitation hypothesis) and/or pollinator landing (the landing-control hypothesis). To examine these two hypotheses, we altered the angle of Commelina communis flowers and examined the efficiency of pollen transfer, as well as the behavior of their visitors. We exposed unmanipulated (horizontal-), upward-, and downward-facing flowers to syrphid flies (mostly Episyrphus balteatus), which are natural visitors to C. communis. The frequency of pollinator approaches and landings, as well as the amount of pollen deposited by E. balteatus, decreased for the downward-facing flowers, supporting both hypotheses. The upward-facing flowers received the same numbers of approaches and landings as the unmanipulated flowers, but experienced more illegitimate landings. In addition, the visitors failed to touch the stigmas or anthers on the upward-facing flowers, leading to reduced pollen export and receipt, and supporting the landing-control hypothesis. Collectively, our data suggested that the horizontal orientation of zygomorphic flowers enhances pollen transfer by both facilitating pollinator recognition and controlling pollinator landing position. These findings suggest that zygomorphic flowers which deviate from a horizontal orientation may have lower fitness because of decreased pollen transfer.SPRINGER, 2009年07月, OECOLOGIA, 160(4) (4), 667 - 674, 英語[査読有り]研究論文（学術雑誌）
- In the carabid genus Carabus subgenus Ohomopterus, diverged body size and genital morphology serve as mechanical reproductive barriers. To elucidate the diverging process of body and genital sizes in Carabus arrowianus, which exhibits marked morphological diversity among geographical populations and may represent an early stage of speciation, we analysed a mitochondrial gene sequence for 1051 individuals from 63 populations and male morphology for 359 individuals from 47 populations. Two discrete morphological groups segregated by geographical barriers were distinguished, one of which possessed smaller bodies and shorter genitalia (S group) than the other (L group), which exhibited larger bodies and exaggerated genitalia. Genetic divergence between the two groups was significant but not large. Phylogeographical and population genetic analyses indicated that the L group was derived from the S group, and a coalescent simulation revealed that the two groups diverged during the latest middle Pleistocene (0.13 million years ago), with a much larger effective population size in the L group than the S group. Because the body size divergence could not be explained by adaptation to climatic conditions and genital morphology is considered to be subject to sexual selection, we postulated that a population division and colonization in favourable habitats caused by the Pleistocene climatic and geographical change might facilitate natural and sexual selection for enlarged body and genital sizes in the L group.WILEY-BLACKWELL PUBLISHING, INC, 2009年04月, MOLECULAR ECOLOGY, 18(7) (7), 1408 - 1421, 英語[査読有り]研究論文（学術雑誌）
- 2009年, Australian Entomologist, 36: 13-20, 英語The genus Pamborus Latreille (Coleoptera: Carabidae) in the Sloane collection and its importance for current taxonomy[査読有り]研究論文（学術雑誌）
- Males of internally fertilizing animals often produce ejaculates consisting of various substances in addition to sperm. Seminal substances can inhibit female remating through physical blocking of the female genitalia or by physiological induction of the female refractory period. We demonstrated that the seminal substances of the ground beetle Leptocarabus procerulus serve as both physical (i.e., mating plugs) and physiological (i.e., substances inducing female refractory behavior) devices for mate guarding. Although females counteract these functions via expulsion of the plug and the delay of the occurrence of refractory behavior, the interplay of these 2 functions can be compensatory and consistently lowers the female remating rate during a postmating period that is important for male fertilization success. Such interplay of 2 defensive strategies may be a male adaptation against female resistance, as predicted by the hypothesis that multiple functions in seminal substances are a historical outcome of an arms race between males and females. Our findings highlight the importance of sexual conflict in the evolution of complex seminal substances.OXFORD UNIV PRESS INC, 2008年11月, BEHAVIORAL ECOLOGY, 19(6) (6), 1173 - 1178, 英語[査読有り]研究論文（学術雑誌）
- 東京都内に広く分布するモンシロチョウ Artogeia rapae (=Pieris rapae)およびスジグロシロチョウ A. melete (=P. melete)の2種のシロチョウについて,東京都内全域において,過去にどのような分布の変遷をたどってきたか調べるために,アンケート調査,文献調査およびフィールド調査を行った.その結果,特別区では,1950年代から1960年代にかけてモンシロチョウが多かったが,1970年代以降スジグロシロチョウが増え始め,1980年代には都心に近い場所でも多数のスジグロシロチョウが目撃されるようになったが,1990年代以降,再びスジグロシロチョウの目撃例が減少し,かわってモンシロチョウの目撃例が増加したことが明らかになった.さらにこのようなモンシロチョウとスジグロシロチョウの分布の変遷は,特別区以外の郊外の市町村や島嶼部でも見られることがわかった.日本鱗翅学会, 2008年, 蝶と蛾, 59: 1-17(1) (1), 1 - 17, 日本語[査読有り]研究論文（学術雑誌）
- Speciation studies seek to clarify the origin of reproductive isolation, the various mechanisms working from mate recognition through postzygotic stages. Asymmetric effects of isolating barriers can result in asymmetrical gene introgression during interspecific hybridization. The flightless ground beetles Carabus yamato and C. albrechti are distributed parapatrically in Japan, showing repeated asymmetrical introgression of mitochondria from C. albrechti to C. yamato. This pattern suggests that reproductive isolation between these species is strong, but incomplete and asymmetric (i.e., weaker for the cross between a C. albrechti female and a C. yamato male). To test this hypothesis, we conducted interspecific mating experiments in the laboratory. The estimates of total reproductive isolation, which occurred mainly at the premating and postmating/prezygotic stages, were high (isolation index = 0.964 for C. yamato female x C. albrechti male and 0.886 for the reciprocal cross), supporting the hypothesis of strong, but incomplete isolation. However, the observed difference between the reciprocal crosses was not sufficiently large to conclude that it caused directional introgression of mitochondria. Instead, we found asymmetry in individual isolating barriers in the postmating/prezygotic stages that coincided with the prediction, perhaps resulting from morphological mismatch of heterospecific genitalia. Although this asymmetry was compensated for by an inverse asymmetry of isolation in the postzygotic stage, the contribution of these individual barriers to total isolation may change for our expectation when considering females mating with multiple heterospecific males.SPRINGER TOKYO, 2007年10月, POPULATION ECOLOGY, 49(4) (4), 337 - 346, 英語[査読有り]研究論文（学術雑誌）
- Diversification of sperm morphology has been investigated in the context of sperm competition, but the adaptive significance of sperm bundles is still unclear. In analyzing 10 taxa of the genus Carabus subgenus Ohomopterus and one related Carabus ground beetles, we found that dimorphic sperm bundles occurred in most species with varied degrees of bimodality, whereas sperm were generally monomorphic. Comparative analyses with phylogenetically independent contrasts revealed that the sizes of large and small sperm bundles evolved more rapidly than, and were not correlated with, the length of sperm, suggesting more intense selection on sperm bundle sizes and their independent responses to different evolutionary forces. The size of large sperm bundles was positively correlated with male genital morphology (pertinent to displacement of rival spermatophores) and postcopulatory guarding duration as well as male body length, suggesting that larger sperm bundles have been favored when the risk of spermatophore displacement is high. Larger sperm bundles may be advantageous because of their ability to migrate more rapidly into the spermatheca. In contrast, no clear association was detected between the small sperm bundle size and mating traits despite its rapid diversification. The present study provides the first record of heteromorphic sperm bundles, the diversity of which may be promoted by sperm competition.SPRINGER, 2007年07月, NATURWISSENSCHAFTEN, 94(7) (7), 543 - 550, 英語[査読有り]研究論文（学術雑誌）
- We analysed evolutionary diversification and covariation in male genitalia and four mating traits related to sexual selection, i.e. testis size, spermatophore size, copulation duration and post-copulatory guarding duration, in Ohomopterus ground beetles using phylogenetically independent contrasts. Male genital size and mating duration have evolved more rapidly than body size and the other traits studied. Male genital size was negatively correlated with copulation duration, suggesting that elongated male genitalia may enable decreased time investment in a single copulation because it is more effective at facilitating spermatophore deposition. Male genital size was positively correlated with spermatophore size, suggesting coevolution between offensive and defensive male mating tactics because the elongated male genitalia may be advantageous in displacement of rivals' plug-like spermatophores, and decreased mating duration may intensify sperm competition. Thus, the remarkable diversity of male genitalia in Ohomopterus may have been facilitated by the interplay between inter- and intrasexual selection processes.WILEY-BLACKWELL, 2007年07月, JOURNAL OF EVOLUTIONARY BIOLOGY, 20(4) (4), 1385 - 1395, 英語[査読有り]研究論文（学術雑誌）
- Marked diversification of genital morphology is common in internally fertilizing animals. Although sexual selection may be the primary process controlling genital evolution, factors promoting genital evolution are controversial, and the genetic background of genital morphology is poorly understood. We analyzed the genetic basis of species-specific genital morphologies in carabid beetles of thesubgenus Ohomopterus (genus Carabus, Carabidae) using two parapatric species with hybrid zones. Biometric analyses on experimental F-1 and backcross populations revealed that inheritance of genital morphology is polygenic. Applying Lande's modification of the Castle-Wright estimator to population means and variances to estimate the minimum number of genes involved, we found that a relatively small number of loci is responsible for species differences in genital morphology. In addition, joint-scaling tests indicated that the additive genetic effect accounts for most interspecific differences in genital traits, but dominance and epistatic genetic effects also play roles. Overall, the genetic basis of male and female genitalia is fairly simple, enabling these traits to respond quickly to selection pressures and to diverge rapidly. Our results provide insight into the diversification of genital morphology in carabid beetles, and will hopefully stimulate further studies on the genetic basis of genitalia, such as mapping of quantitative trait loci affecting species-specific genital morphology.NATURE PUBLISHING GROUP, 2007年06月, HEREDITY, 98(6) (6), 385 - 391, 英語[査読有り]研究論文（学術雑誌）
- Sperm displacement is an effective male mating tactic of sperm competition to ensure paternity. However, the effectiveness of sperm displacement may depend on defensive mating tactics employed by rival males and also may be affected by females. To test this hypothesis, I conducted double-mating experiments with microsatellite paternity analyses using the ground beetle Carabus insulicola. First, I examined how often spermatophore displacement by the second male occurs. Spermatophore deposition success of the second male was high (85%) as was that of the first male (100%), in which 80% of the second males displaced the first male's spermatophore, suggesting that spermatophore displacement is a highly effective offensive tactic of sperm competition in C. insulicola. Second, I examined the effects of postcopulatory guarding by the first male and remating interval and sperm utilization pattern of the female on fertilization success of the second male. P-2 values were moderate on average (0.30-0.57) and negatively correlated with postcopulatory guarding duration of the first male. Sperm use by the female was temporally nonrandom, such that sperm of the second male tended to be used first, although there was no significant effect of female-remating interval. These results suggest that the effectiveness of spermatophore displacement depends on postcopulatory guarding by the first male and the sperm utilization pattern of the female. These findings provide empirical evidence for the conflicting nature of selection for male offensive and defensive tactics in sperm competition and significant contribution of females therein.OXFORD UNIV PRESS INC, 2007年05月, BEHAVIORAL ECOLOGY, 18(3) (3), 628 - 634, 英語[査読有り]研究論文（学術雑誌）
- We analyzed the intraspecific gene genealogies of three Leptocarabus ground beetle species (L. seishinensis, L. semiopacus, L. koreanus) in South Korea using sequence data from the mitochondrial cytochrome oxidase subunit I (COI) and nuclear 28S rRNA (28S) genes, and compared phylogeographical patterns among the species. The COI data detected significant genetic differentiation among local populations of all three species, whereas the 28S data showed genetic differentiation only for L. seishinensis. The clearest differentiation of L. seishinensis among local populations was between the northern and southern regions in the COI clades, whereas the 28S clade, which likely indicates relatively ancient events, revealed a range expansion across the northern and southern regions. Leptocarabus semiopacus had the most shallow differentiation of the COI haplotypes, and some clades occurred across the northern and southern regions. In L. koreanus, four diverged COI clades occurred in different regions, with partial overlaps. We discuss the difference in phylogeographical patterns among these Leptocarabus species, as well as between these and other groups of carabid beetles in South Korea.ZOOLOGICAL SOC JAPAN, 2006年09月, ZOOLOGICAL SCIENCE, 23(9) (9), 745 - 754, 英語[査読有り]研究論文（学術雑誌）
- 2006年, Australian Journal of Entomology, 45: 44-54, 44 - 54, 英語Four new species of Australian Pamborus Latreille (Coleoptera, Carabidae) carabid beetles[査読有り]研究論文（学術雑誌）
- We investigated the species status and intraspecific phylogeography in South Korea of two ground beetle species, Coptolabrus jankowskii and Coptolabrus smaragdinus (Coleoptera: Carabidae), using statistical parsimony networks and nested clade analyses based on sequences from the mitochondrial cytochrome oxidase subunit I (COI) and nuclear phosphoenolpyruvate carboxykinase (PepCK) and wingless (Wg) genes. Although traditional parsimony tree construction generally failed to resolve interspecific relationships and construct biologically meaningful genealogies, analysis using statistical parsimony networks yielded statistically significant inter- and intraspecific genealogical structures. We found that although these two species represent a notable case of trans-species polymorphisms in both mitochondrial and nuclear gene sequences, their status as separate species was evidenced by the nonrandom association between species and nested clades at various nesting levels. The exceptional occurrence of shared identical or very similar COI sequences was considered to be the result of introgressive hybridization. In addition, range expansion and fragmentation events across the Korean Peninsula and adjacent islands were inferred from nested clade phylogeographical analyses. The COI gene revealed the geographical divergence of major eastern and western clades and historical biogeographical events within each major clade, whereas the nuclear PepCK gene, which did not reveal corresponding east west clades, indicated past fragmentation and range expansion across wide areas that may have been the result of older biogeographical events. Thus, phylogeographical inferences drawn from analyses of mitochondrial and nuclear genes can reveal different and potentially complementary information about phylogeographical processes.BLACKWELL PUBLISHING, 2005年10月, MOLECULAR ECOLOGY, 14(12) (12), 3823 - 3841, 英語[査読有り]研究論文（学術雑誌）
- Morphological, genetic and behavioural analyses of a hybrid zone between the ground beetles Carabus lewisianus and C albrechti (Coleoptera, Carabidae): asymmetrical introgression caused by movement of the zone?We analysed the putative hybrid zone between the ground beetles Carabus lewisianus Breuning and C. albrechti Morawitz from the Kanto and Tanzawa Mountains in central Honshu, Japan, using morphological, genetic, and behavioural data. Canonical discriminant analysis of three external and five genital morphological characters revealed an apparent morphological gap, suggesting restricted sympatry in the contact zone. RFLP and molecular phylogenetic analyses of the mitochondrial gene ND5 revealed that haplotypes originating from C. lewisianus introgressed extensively into C. albrechti. Fitting tanh curves to morphological (external and genital) and genetic characters showed differential cline widths and centres, suggesting stronger selection for genital morphology with narrower cline widths, and extensive introgression of the nuclear genes responsible for external characters relative to those controlling genital morphology into the range of C. albrechti. Linkage disequilibria between the morphological characters did not differ from zero, suggesting sufficient recombination occurred in the zone but the effect of small sample sizes was not negligible. Mate choice trials demonstrated that males showed a weak but not significant preference for conspecific mates. We interpret these patterns as evidence for (1) partial reproductive isolation presumably by postmating processes; (2) selection against intermediate genital morphologies, and (3) independent responses of the characters to different evolutionary forces that may cause movement of genital clines and introgression of the mitochondrial gene and external morphology. The movement of the boundaries may have resulted in the particular species distribution patterns in the study area. (c) 2005 The Linnean Society of LondonBLACKWELL PUBLISHING, 2005年09月, BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, 86(1) (1), 79 - 94, 英語[査読有り]研究論文（学術雑誌）
- The phylogeny of carabid beetles in the genus Pamborus (Coleoptera: Carabidae), which is endemic to Australia, was studied using one nuclear (phosphoenolpyruvate carboxykinase) and two mitochondrial (16S ribosomal RNA and NADH dehydrogenase subunit 5) gene sequences, with a cladistic analysis of morphological data. Fourteen species that were morphologically distinguishable were used as ingroup taxa, and Maoripanborus fairburni from New Zealand was assigned as the outgroup. Simultaneous analysis of three gene sequences resulted in well-resolved trees that were largely consistent with the cladogram generated from the morphological data. Based on a clock-like tree calibrated to the New Zealand-Australia/Antarctica split 85 million years ago, it was estimated that extant Pamborus differentiated after the Oligocene, primarily since the mid-Miocene with the onset of a more and climate and forest fragmentation in Australia. The ancestral Pamborus may have been small, whereas medium to large Pamborus species with exaggerated male genitalia constitute derived groups and are now dominant. (c) 2005 Elsevier Inc. All rights reserved.ACADEMIC PRESS INC ELSEVIER SCIENCE, 2005年08月, MOLECULAR PHYLOGENETICS AND EVOLUTION, 36(2) (2), 391 - 404, 英語[査読有り]研究論文（学術雑誌）
- COLEOPTERISTS SOC, 2004年06月, COLEOPTERISTS BULLETIN, 58(2) (2), 271 - 272, 英語Parasitism of Carabus kimurai (Ishikawa) (Coleoptera : Carabidae) by the endoparasitic fly Zaira cinerea fallen (Diptera : Tachinidae) and its effect on male reproductive capability[査読有り]研究論文（学術雑誌）
- Conservation programs in urban ecosystems need to determine the genetic background in populations of urban dwellers. We examined the genetic diversity and structure of Pieris rapae and P. melete using AFLP markers, and compared them between species and between urban and rural environments. As a result: (i) in both species, there was no reduction in genetic diversity within urban populations by direct comparison of diversity measurements, although the analysis of molecular variance suggested significant reductions in the variance within seasonal subpopulations in urban populations; (ii) P. rapae retained greater genetic diversity within species and populations; (iii) populations of both species showed significant genetic differentiation, and P. melete was more strongly subdivided; (iv) in both species, geographically close populations did not cluster with one another in the UPGMA analysis; (v) there was no genetic isolation due to geographical distance in either species; (vi) the genetic composition of seasonal subpopulations differed in urban populations of both species, and the genetic distances among subpopulations were correlated with seasonal differences in P. rapae and with temporal differences in P. melete. These results indicate that the genetic diversity in urban populations of both species was reduced at times, but was maintained by dispersal from genetically differentiated populations. Differences in the ability and mode of dispersal in the two species may be reflected in the degree of population subdivision and patterns of seasonal change in the genetic composition.WILEY-BLACKWELL, 2004年02月, MOLECULAR ECOLOGY, 13(2) (2), 245 - 258, 英語[査読有り]研究論文（学術雑誌）
- Experimental analysis of the effect of genital morphology on insemination success in the ground beetle Carabus insulicola (Coleoptera Carabidae)The hypothesis that the elaborated genitalia of male insects serve to improve insemination, success were tested using the ground beetle Carabus insulicola. To enhance variation in genital size, the genital hooks of experimental males were cut, and these males were then mated with virgin females. Logistic regression showed that the length of the male genital hook affected insemination success. Males with a shorter genital hook tended not to deposit spermatophores at the proper site, and failed to transfer sperm into the spermatheca. Therefore, the male genital hook serves to increase insemination success by depositing a spermatophore at the site where sperm are likely to be transferred. The duration of copulation and post-copulatory guarding may also be explained by these determinants. Stepwise regress-ions indicated that the occurrence of ejaculation, and the location of the spermatophore determined the duration of copulation and post-copulatory guarding, respectively.UNIVERSITA DEGLI STUDI DI FIRENZE, 2003年04月, ETHOLOGY ECOLOGY & EVOLUTION, 15(1) (1), 51 - 61, 英語[査読有り]研究論文（学術雑誌）
- Mating behavior, insemination and sperm transfer in the ground beetle Carabus insulicolaMating behavior and the processes of insemination and sperm transfer in the ground beetle Carabus insulicola were analyzed. C. insulicola has elaborate genitalia, in which the strongly sclerotized male copulatory piece is inserted into the female vaginal appendix in copula. During mating, I observed pre-copulatory struggles of males and females, as well as delays in ejaculation, suggesting the presence of intersexual conflicts. Insemination was achieved with a spermatophore, which strongly adhered to the openings of the spermatheca, common oviduct, and vaginal appendix. The spermatophore dissolved after copulation, and sperm were transferred into the spermatheca within three hours after copulation. Sperm bundles were contained within the testes and spermatophores, but free spermatozoa were found in the spermatheca.ZOOLOGICAL SOC JAPAN, 2002年09月, ZOOLOGICAL SCIENCE, 19(9) (9), 1067 - 1073, 英語[査読有り]研究論文（学術雑誌）
- オサムシ類の体サイズの地理的変異:気候適応と種間相互作用がもたらすパターンThe geographic variation in body size of an insect species may reflect not only differential adaptation to local climatic conditions but also evolutionary consequences of interaction with closely related species. Thus, both intra- and interspecific body size variations need to be analyzed to understand evolutionary significance of geographic pattern of body size variation in a group of closely related species. Here, we introduce geographic patterns of intra- and interspecific body size variation in two groups of carabid beetles in Japan, Leptocarabus and Ohomopterus (the subtribe Carabina, Carabidae) and discuss the possible factors leading to the observed patterns. Body size of a species generally decreases with decreasing warmth of the habitat (the converse of the Bergmann's rule). However, coexisting related species at sympatric zones show separation in body size, leading to deviation from overall clinal trend of body size variation within species. Probably, interspecific interaction through reproductive interference at the secondary contacts has played a role in determining the combination of species that could coexist or affected the body size differentiation per se.日本昆虫学会, 2002年, Japanese Journal of Entomology (New Series), 5: 88-97(3) (3), 88 - 97, 日本語[査読有り]
- Microsatellite DNA markers for the ground beetle Carabus insulicolaTwelve microsatellite DNA loci were newly isolated from the ground beetle Carabus insulicola, endemic to Japan, for studying mating systems. Phage vector was used for establishing genomic DNA library, and positive clones were screened with P-32-labelled probes. Primer sequences and annealing temperature for PCR amplification were determined for each locus. Allelic polymorphism of each locus, number of alleles and observed and expected heterozygosities, were investigated based on 24 individuals. Similarity between observed and expected heterozygosities suggests random mating, and relatively low observed heterozygosity found in two loci may be due to the presence of null alleles.BLACKWELL PUBLISHING LTD, 2001年09月, MOLECULAR ECOLOGY NOTES, 1(3) (3), 128 - 130, 英語[査読有り]研究論文（学術雑誌）
- Interspecific body size differentiation in species assemblages of the carabid subgenus Ohomopterus in JapanGeographic variation and interspecific differentiation in body size (body length) were analyzed for 15 species of the carabid subgenus Ohomopterus (genus Carabus; Coleoptera, Carabidae) in Japan. Local species assemblages of this subgenus consist of up to 5 species of different size classes. These beetles exhibited sexual dimorphism in body size where females are larger than males, except Carabus uenoi, in which the male and female sizes were equivalent, possibly because of the exaggerated male genitalia. In 9 of 15 species, there was a positive correlation between mean body size and annual mean temperature of habitat, representing the converse of Bergmann's rule. However, in some cases this correlation does not hold over the range of a species because of regional differences. When allopatric and sympatric populations were compared, allopatric populations of Carabus albrechti and C. japonicus had larger bodies than sympatric populations. These intraspecific differences may have resulted from character displacement. In each local assemblage with 2 or more species, there was little interspecific overlap of body size, although the body size ratio between two species with adjacent body sizes seldom showed strict constancy. The mean size ratio between 2 adjacent species in an assemblage was reduced with the number of species, whereas the size ratio of the largest to smallest species in an assemblage increased with the number of species (i.e., the expansion of body size range). These results indicate that the body size of Ohomopterus species may have evolved in response to both climatic conditions and interspecific interactions. Because each species or species group represents the same size class over the distribution range and similar-sized species are parapatric or allopatric, the interspecific segregation in body size in local assemblages may have resulted mainly from a size assortment process during colonization.SPRINGER-VERLAG TOKYO, 2000年12月, POPULATION ECOLOGY, 42(3) (3), 279 - 291, 英語[査読有り]研究論文（学術雑誌）
- 2000年, Tokyo Metropolitan University Bulletin of Natural History, 4: 1-32, 1 - 32, 英語Phylogeny of the subgenus Ohomopterus (Coleoptera, Carabidae, genus Carabus): A morphological aspect[査読有り]研究論文（学術雑誌）
- 2000年, Entomological Science, 3: 309-320, 309 - 320, 英語Geographic variation in body size of some Japanese Leptocarabus species (Coleoptera, Carabidae): The "toppled-domino pattern" in species along a geographic cline[査読有り]研究論文（学術雑誌）
- 1997年, Tokyo Metropolitan University Bulletin of Natural History, 3: 55-99, 55 - 99, 英語Subspeciation and distribution pattern of Carabus albrechti Morawitz in Japan (Coleoptera, Carabidae)[査読有り]研究論文（学術雑誌）
- New subspecies of Carabus albrechti Morawitz (Coleoptera, Carabidae) from JapanFour new subspecies of Carabus (Ohomopterus) albrechti are described : C. a. hidakanus nov. and C. a. itoi nov. from Hokkaido, and C. a. echigo nov. and C. a. awashimae nov. from the Japan Sea side of central Honshu and Awashima Is., respectively.日本動物分類学会, 1996年, Species Diversity, 1: 39-48(1) (1), 39 - 48, 英語[査読有り]研究論文（学術雑誌）
- 2025年03月, 昆虫と自然, 60(4) (4), 15 - 19オサムシの交尾器形態の形質置換と種分化[招待有り]記事・総説・解説・論説等（学術雑誌）
- 2024年03月, 昆虫（ニューシリーズ）, 27(1) (1), 50 - 51＜書評＞マーリーン・ズック，リー・W・シモンズ 著，沼田英治 監訳，遠藤淳 訳（2023）「なぜオスとメスは違うのか 性淘汰の科学」大修館書店[査読有り]書評論文,書評,文献紹介等
- 2017年, 昆虫と自然, 52(13) (13), 30 - 32, 日本語昆虫の種分化研究の現状と今後の展開−自然史とゲノミクスが出会う時[招待有り]記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）
- ニューサイエンス社, 2016年, 昆虫と自然, 51(12) (12), 6 - 10, 日本語都市環境と分子生態学を通して見るシロチョウの分布と移動[招待有り]記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）
- ニューサイエンス社, 2011年, 昆虫と自然, 46 (3): 9-13(3) (3), 9 - 13, 日本語オサムシの交尾器はなぜ多様なのか-性淘汰と遺伝的基盤[招待有り]記事・総説・解説・論説等（学術雑誌）
- 2011年, 昆虫と自然, 46 (3): 13-14, 日本語オオオサムシ亜属の交尾のしくみ記事・総説・解説・論説等（学術雑誌）
- 2005年, Center for Ecological Research News, 87: 17-19, 17 - 19, 日本語都市環境におけるモンシロチョウとスジグロチョウの集団遺伝学記事・総説・解説・論説等（学術雑誌）
- 2005年, 歩く宝石オサムシ - 飛ばない昆虫のふしぎ発見, Pp. 76-77, 日本語「錠と鍵」の機能記事・総説・解説・論説等（学術雑誌）
- 2005年, 歩く宝石オサムシ - 飛ばない昆虫のふしぎ発見, Pp. 72-75, 日本語オサムシを分ける錠と鍵記事・総説・解説・論説等（学術雑誌）
- ニュ-・サイエンス社, 2001年, 昆虫と自然, 36 (2): 24-28(2) (2), 24 - 28, 日本語性選択から見たオサムシ交尾器の機能と進化[招待有り]記事・総説・解説・論説等（学術雑誌）
- ニュ-・サイエンス社, 1998年, 昆虫と自然, 33 (9): 38-41(9) (9), 38 - 43, 日本語ホソアカガネオサムシの生活史
■ 講演・口頭発表等
- 日本生態学会第72回全国大会, 2025年03月オサムシにおける防御分泌物に対する行動的反応ポスター発表
- 日本生態学会第72回全国大会, 2025年03月マヤサンオサムシの雄交尾器における形状と弾性の生殖的形質置換ポスター発表
- 日本生態学会第72回全国大会, 2025年03月クロナガオサムシとオオクロナガオサムシの自種選好性と種間交雑ポスター発表
- 日本生態学会第72回全国大会, 2025年03月オオオサムシ亜属における雄交尾器の大型化に関わる進化発生機構ポスター発表
- 第26回日本進化学会神奈川大会, 2024年08月オオオサムシ亜属における交尾器サイズの多様化に関わる発生過程とその組織学的背景ポスター発表
- 日本生態学会第71回全国大会, 2024年03月交尾器形態の生殖的形質置換：壊れにくく異種を識別しやすい柔らかい雄交尾器の進化ポスター発表
- 日本生態学会第71回全国大会, 2024年03月オオオサムシ亜属における交尾器の巨大化に関わる進化発生学的要因ポスター発表
- 日本動物行動学会第42回大会, 2023年11月共食いされなかった雄の再交尾は配偶者防衛として機能するのかポスター発表
- 日本動物行動学会第42回大会, 2023年11月チョウセンカマキリにおける栄養状態と交尾履歴が性的共食いにおよぼす影響ポスター発表
- 第70回日本生態学会大会, 2023年03月昆虫の捕脚形態における収斂進化の定量的解析ポスター発表
- 第70回日本生態学会大会, 2023年03月チョウセンカマキリにおける性的共食いに伴う交尾の延長と配偶者防衛の強化ポスター発表
- 第70回日本生態学会大会, 2023年03月チョウセンカマキリにおける摂食量と肥満度が性的共食いに及ぼす影響ポスター発表
- 日本動物行動学会第41回大会, 2022年11月, 日本語チョウセンカマキリにおける摂食量と肥満度が性的共食いにおよぼす影響口頭発表（一般）
- 日本動物行動学会第41回大会, 2022年11月, 日本語チョウセンカマキリにおける性的共食いに伴う交尾の延長と配偶者防衛の強化口頭発表（一般）
- 日本生態学会第69回全国大会 自由集会「一寸の虫にもラブの魂 IV」, 2022年03月, 日本語異種と対立とカタチ：種間相互作用と性淘汰が織りなす交尾器形態の多様化[招待有り]口頭発表（招待・特別）
- 日本生態学会第69回全国大会, 2022年03月, 日本語チョウセンカマキリのメスにおける性的共食いの栄養的利益ポスター発表
- 日本生態学会第69回全国大会, 2022年03月チョウセンカマキリの精子競争における雄交尾器形態への性淘汰の検出ポスター発表
- 日本生態学会第69回全国大会, 2022年03月, 日本語雄交尾器の巨大化に伴うボディーパーツ間の相関構造の変化ポスター発表
- 日本生態学会第69回全国大会, 2022年03月, 日本語昆虫の捕脚の進化における形態学的特徴ポスター発表
- 日本生態学会第69回全国大会, 2022年03月, 日本語生殖的形質置換に伴う雄交尾器の柔らかさの進化ポスター発表
- 日本進化学会第23回大会シンポジウム, 2021年08月性的対立をもたらす交尾器形態が野生個体群動態と分布域形成におよぼすインパクト[招待有り]口頭発表（招待・特別）
- 日本生態学会第68回大会, 2021年03月種特異的な交尾器部位の極端な大型化に関わる発生過程ポスター発表
- 日本生態学会第68回大会, 2021年03月マヤサンオサムシの交尾器形態にはたらく強化淘汰の検出ポスター発表
- 日本生態学会第68回大会, 2021年03月チョウセンカマキリのメスにおける性的共食いの栄養的利益ポスター発表
- 日本生態学会第68回大会, 2021年03月性的共食いを受けた雄の交尾行動調節とその適応的意義ポスター発表
- 第67回日本生態学会大会, 2020年03月, 日本語マヤサンオサムシの交尾器形態にはたらく強化淘汰 の検出ポスター発表
- 第67回日本生態学会大会, 2020年03月, 日本語種特異的な交尾器部位の極端な大型化に関わる発生過程ポスター発表
- 第67回日本生態学会大会, 2020年03月, 日本語チョウセンカマキリのメスにおける性的共食いの栄養的利益ポスター発表
- 第67回日本生態学会大会, 2020年03月, 日本語雄カマキリの交尾行動に対する性的共食いの影響ポスター発表
- Genital Evolution: genes, function and diversification, 35th the society of population ecology meeting, 2019年09月, 英語, 国際会議Causes and consequences of genital diversification in Ohomopterus ground beetles[招待有り]口頭発表（招待・特別）
- 第66回日本生態学会大会, 2019年03月, 日本語, 神戸コンベンションセンター, 国内会議交尾器形態の形質置換と交雑コスト回避の検証ポスター発表
- 第66回日本生態学会大会, 2019年03月, 日本語, 神戸コンベンションセンター, 国内会議寄主植物形質に応じた植食性昆虫の防衛形態の進化ポスター発表
- 第66回日本生態学会大会, 2019年03月, 日本語, 神戸コンベンションセンター, 国内会議マヤサンオサムシの地理的変異と初期種分化 種間相互作用による種分化仮説の検証ポスター発表
- 第66回日本生態学会大会, 2019年03月, 日本語, 神戸コンベンションセンター, 国内会議マイクロCTを用いた多様な交尾器形態の発生過程の解明ポスター発表
- 第66回日本生態学会大会, 2019年03月, 日本語, 神戸コンベンションセンター, 国内会議フタイロカミキリモドキにおける性的二型 形質の緯度クラインと共進化ポスター発表
- 第66回日本生態学会大会, 2019年03月, 日本語, 神戸コンベンションセンター, 国内会議チョウセンカマキリの繁殖フェノロジーと雌の 交尾頻度の推定ポスター発表
- 日本蜘蛛学会第50回大会, 2018年08月, 日本語, 広島大学, 国内会議生態と進化の間に介在する性的対立とオサムシの交尾器[招待有り]口頭発表（招待・特別）
- 日本進化学会第20回大会, 2018年08月, 日本語, 東京大学, 国内会議交尾器形態の形質置換と交雑コスト回避の検証口頭発表（一般）
- 日本進化学会第20回大会, 2018年08月, 日本語, 東京大学, 国内会議マイクロCTを用いた多様な交尾器形態の発生過程の解明ポスター発表
- 第65回日本生態学会大会, 2018年03月, 日本語, 札幌コンベンションセンター（北海道札幌市）, 国内会議物理的防衛と隠蔽効果のトレードオフによる昆虫の形態多様化ポスター発表
- 第65回日本生態学会大会, 2018年03月, 日本語, 札幌コンベンションセンター（北海道札幌市）, 国内会議琵琶湖産スジエビの生活史多型に関連した遺伝的構造の解明ポスター発表
- 第65回日本生態学会大会, 2018年03月, 日本語, 札幌コンベンションセンター（北海道札幌市）, 国内会議発達したオス後脚は性的対立によって進化したのか:個体群入れ替え配偶実験による検証ポスター発表
- 第65回日本生態学会大会, 2018年03月, 日本語, 札幌コンベンションセンター（北海道札幌市）, 国内会議局所環境変動に対する種の応答に基づく群集変化予測ポスター発表
- 第65回日本生態学会大会, 2018年03月, 日本語, 札幌コンベンションセンター（北海道札幌市）, 国内会議マヤサンオサムシの交尾器形態の地理的変異は生殖的形質置換か？：雌雄両面からの検証ポスター発表
- 第65回日本生態学会大会, 2018年03月, 日本語, 札幌コンベンションセンター（北海道札幌市）, 国内会議マイクロCTを用いた多様な交尾器形態の発生過程の解明ポスター発表
- 第65回日本生態学会大会, 2018年03月, 日本語, 札幌コンベンションセンター（北海道札幌市）, 国内会議カマキリを利用するハリガネムシの性依存的寄生戦略口頭発表（一般）
- 第64回日本生態学会大会, 2017年03月, 日本語, 早稲田大学, 国内会議頭部神経による抑制支配からの解放が雄カマキリの適応的な交尾行動調節をもたらすポスター発表
- 第64回日本生態学会大会, 2017年03月, 日本語, 早稲田大学, 国内会議昆虫の多様な防御形態：捕食様式の違いに対応したジェネラリスト形態とスペシャリスト形態の進化ポスター発表
- 第64回日本生態学会大会, 2017年03月, 日本語, 早稲田大学, 国内会議局所環境の変化による歩行虫群集の時間的変化と広域の群集評価へのインパクトポスター発表
- 第64回日本生態学会大会, 2017年03月, 日本語, 早稲田大学, 国内会議マヤサンオサムシの交尾器形態の地理的変異は生殖的形質置換か？：交雑のコストとコスト回避の可能性ポスター発表
- 第64回日本生態学会大会, 2017年03月, 日本語, 早稲田大学, 国内会議チョウセンカマキリに対するハリガネムシの寄生コストと形態改変ポスター発表
- 第64回日本生態学会大会, 2017年03月, 日本語, 早稲田大学（東京都）, 国内会議eDNA 分析で明らかになった琵琶湖産スジエビの時空間的分布ポスター発表
- 第25回国際昆虫学会議, 2016年09月, 英語, Orlando, 国際会議Detecting divergent sexual selection operating upon divergent genital morphologies口頭発表（一般）
- 日本生態学会第63回大会, 2016年03月, 日本語, 東北大学, 国内会議対捕食者適応による昆虫の外部形態の進化：捕食パターンの違いによる影響の検証ポスター発表
- 日本生態学会第63回大会, 2016年03月, 日本語, 東北大学, 国内会議多様な交尾器形態にはたらく分化性淘汰の検出：距離測定法と幾何学的形態測定学法の違いと相補性[招待有り]口頭発表（招待・特別）
- 日本生態学会第63回大会, 2016年03月, 日本語, 東北大学, 国内会議韓国白頭大幹における環境変動に応じた歩行虫群集の変化ポスター発表
- 日本生態学会第63回大会, 2016年03月, 日本語, 東北大学, 国内会議ハリガネムシに寄生されたカマキリの繁殖行動ポスター発表
- 日本生態学会第63回大会, 2016年03月, 日本語, 東北大学, 国内会議オサムシの精子競争と多型的な集合精子の謎[招待有り]口頭発表（招待・特別）
- 第40回日本比較内分泌学会大会・日本比較生理生化学会第37回大会合同大会, 2015年12月, 日本語, 広島大学, 国内会議Sexual conflict mediates mating behavior of a sexually cannibalistic praying mantid[招待有り]口頭発表（招待・特別）
- 第62回日本生態学会大会, 2015年05月, 日本語, 鹿児島大学, 国内会議狩蜂の獲物サイズ選好性：巣穴サイズによる制限の可能性口頭発表（一般）
- 第62回日本生態学会大会, 2015年05月, 日本語, 鹿児島大学, 国内会議交尾器形態の多様化をもたらす性淘汰の検出ポスター発表
- 第62回日本生態学会大会, 2015年05月, 日本語, 鹿児島大学, 国内会議韓国五台山における局所環境変動に応じた歩行虫群集の変化ポスター発表
- 動物行動学会第33回大会, 2014年11月, 日本語, 長崎大学, 国内会議チョウセンカマキリの性的共食い：リスクアセスメントと終末投資[招待有り]口頭発表（招待・特別）
- 日本進化学会第16回大阪大会, 2014年08月, 日本語, 高槻現代劇場, 国内会議性的対立の人口学的帰結：雌雄交尾器の不均衡が集団サイズを低下させる口頭発表（招待・特別）
- 第61回日本生態学会大会, 2014年03月, 日本語, 広島大学, 国内会議性的対立の人口学的帰結：雌雄交尾器の不均衡が集団サイズを低下させるポスター発表
- 第61回日本生態学会大会, 2014年03月, 日本語, 広島大学, 国内会議カメノコハムシ亜科の分子系統と特異な外部形態の起源ポスター発表
- 日本動物行動学会第32回大会, 2013年11月, 日本語, 広島大学, 国内会議多様な交尾戦略を持つオオオサムシ亜属の雄の精子投資調節ポスター発表
- 日本動物行動学会第32回大会, 2013年11月, 日本語, 広島大学, 国内会議クロナガオサムシの再交尾抑制をもたらす精液物質は濃すぎると効かないポスター発表
- 日本動物行動学会第32回大会, 2013年11月, 日本語, 広島大学, 国内会議オオオサムシ亜属の雌雄交尾器形態変異に基づく進化仮説の検証ポスター発表
- 第60回日本生態学会大会, 2013年03月, 日本語, 国内会議特異な形態を持つカメノコハムシ亜科の分子系統と形態進化プロセスポスター発表
- 第60回日本生態学会大会, 2013年03月, 日本語, 国内会議チョウセンカマキリの性的共食いによる交尾時間延長が精子競争に与える影響ポスター発表
- 第60回日本生態学会大会, 2013年03月, 日本語, 国内会議イワワキオサムシにおける雄の戦略的射精と父性獲得ポスター発表
- 日本動物行動学会第31回大会, 2012年11月, 日本語, 国内会議チョウセンカマキリの性的共食いに伴う交尾時間延長の適応的意義ポスター発表
- 日本動物行動学会第31回大会, 2012年11月, 日本語, 国内会議イワワキオサムシにおける雌の交尾経験に対応した雄の投資調節ポスター発表
- 第56回日本応用動物昆虫学会大会, 2012年03月, 日本語, 近畿大学農学部、奈良市, 国内会議ギンケハラボソコマユバチMeteorus pulchricornisの産雌性単為生殖メカニズムの解明ポスター発表
- 南アルプス学会, 2024年04月 - 2025年03月, 研究代表者南アルプス深南部におけるオサムシの三つどもえ交雑帯の実態解明
- 日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 神戸大学, 2021年04月01日 - 2024年03月31日比較ゲノミクスと進化発生学から紐解く機械的生殖隔離の強化と種分化本研究は，交雑に起因する交尾器形態の分化と，それに伴う種分化の過程を検証する．交尾器形態の違いが機械的生殖隔離をもたらすオオオサムシ類について，これまで進められてきた研究をさらに発展させ，種分化過程における交尾器形態とゲノムの分化機構と，形態の遺伝発生学的基盤を明らかにすることを目的とする. （1）種間交雑に伴う形態とゲノムの分化機構の解明：分布を接する近縁種を対象に，行動実験によって交尾器形態の形質置換をもたらした自然淘汰を定量する．集団ゲノミクス解析により，自然淘汰と遺伝子流動の影響下にあるゲノムの分化動態を解明する．これまで，他種と接触し交尾器形態に形質置換が見られる複数の個体群において，より種間差の大きい形態変異が種間交尾のコスト（交尾器の損傷，交尾時間のロス）を低減しうるという自然淘汰を検出した．また，比較ゲノム解析のためのサンプリングを行い，ゲノムシーケンスデータを蓄積した． （2）遺伝発生学的基盤の解明：マイクロCTとin situ hybridizationを用いた形態形成・遺伝子発現解析により，交尾器形態の発生過程と，形態の種間差に関わる遺伝的背景を解明する．これまで，最も巨大化した交尾器を持つドウキョウオサムシの発生過程サンプルを取得し，マイクロCTによる形態の観察と測定を進めている．また，in situ hybridizationの実験系を構築するため，ポジティブコントロールのアクチン遺伝子を使って条件設定を進めつつある．
- 科学研究費補助金／基盤研究(B), 2017年04月 - 2021年03月, 研究代表者競争的資金
- 日本学術振興会, 科学研究費助成事業 基盤研究(A), 基盤研究(A), 京都大学, 2015年04月01日 - 2020年03月31日地表性甲虫類の分散・種多様化過程における飛翔能力退化の役割を解明するために，飛翔多型をもつハンミョウ科，オサムシ亜科，ヒラタシデムシ亜科を対象として分子系統地理解析を行った．各系統群の分化年代はジュラ紀から白亜紀と推定され，いずれもアフリカ，南米，オーストラリアなどゴンドワナ起源の陸地には分岐の深い系統が分布し，それらの種多様性は低い傾向があった．飛翔能力の退化は，オサムシ亜科では大部分の系統群，ハンミョウ科では基部系統群の大部分と派生的系統群の一部，ヒラタシデムシ亜科では派生的系統群のみに見られ，ハンミョウ類では少数種の依存固有性，他の２グループでは種多様化と結びついていた.
- 科学研究費補助金／基盤研究(B), 2016年04月 - 2020年03月, 研究代表者競争的資金
- 日本学術振興会, 科学研究費助成事業 基盤研究(C), 基盤研究(C), 鳴門教育大学, 2016年04月01日 - 2019年03月31日フタイロカミキリモドキ（鞘翅目，カミキリモドキ科）のオスは太く発達した後脚をもち，この後脚を用いて激しく抵抗するメスを把握し交尾に至る。オス後脚の太さは個体群によって異なり，奄美群島の個体群は他の個体群に較べて極端に細く，奄美群島を除くと緯度が低い個体群ほど後脚が太くなる傾向がある。配偶実験により，与那国や鳴門の個体群ではより太い後脚をもつオスが交尾に有利であるのに対して，奄美の個体群ではより細い後脚をもつオスが交尾に成功することがわかった。また各地の個体群間の系統樹を作成してみると，後脚が細い奄美群島の個体群は後脚が比較的太い沖縄本島周辺の個体群から派生的に分岐したことが明らかとなった。
- 日本学術振興会, 科学研究費助成事業, 挑戦的萌芽研究, 神戸大学, 2015年04月01日 - 2018年03月31日生活の質を考慮した生態系サービスの評価方法に関する学際研究本研究によって、六甲山系の生態系を調査対象フィールドとし、生態学的研究による生態系サービス源の可視化とデータ集約を通じて、生態系サービス評価を行った。特に評価主体の多様性に注目し、自然経験の消失といった都市特有のライフスタイルの変化がもたらす生態系サービス評価への影響が明らかにされた。また、生活の質に注目した心理学的要素を取り入れた生態系サービス評価として定量的な評価を行った。その結果、心理学的測度と厚生測度の相違、環境や生態系の認知プロセス、都市化とライフスタイル変化を考慮することが、生態系サービス評価において重要であることが示された。
- 日本学術振興会, 科学研究費助成事業 基盤研究(C), 基盤研究(C), 東京学芸大学, 2014年04月01日 - 2017年03月31日同所的に生育している近縁2種（シソ科ヤマハッカ属）を用いて，生殖隔離，繁殖干渉と形質置換の有無を明らかにすることを目的とした。自然状態の種子生産は異所的集団より同所的集団の方が低くなり，同所的集団の神戸岩では2種の開花ピークがずれていたことから，形質置換が生じていることが示唆された。核DNAを用いたヤマハッカ属の系統解析の結果，種ごとにまとまりがみられ，花筒長変異は複数の系統で生じていた。
- 日本学術振興会, 科学研究費助成事業 基盤研究(C), 基盤研究(C), 首都大学東京, 2013年04月01日 - 2017年03月31日クモバチ科の原始社会性の進化的起源を明らかにするため，キマダラズアカクモバチのメスの営巣・社会行動を調べた．その結果，複数メスが同一巣で営巣する（共巣性の）可能性があり，また，一部のメスは営巣期間中に巣を変えることがわかった．また，約100m離れた2営巣地で活動する個体間の遺伝的分化を解析した結果，メスは営巣地間で遺伝的に分化しているが，オスはあまり分化していないことがわかった．営巣地間の遺伝的交流はオスによって行われ得るものの，同じ営巣地の個体は遺伝的類縁性が高いと考えられる．本種の原始社会性の進化と維持には，巣場所への定着性（低移動性）による血縁集団の形成が関与している可能性がある．
- 科学研究費補助金／基盤研究(C), 2012年04月 - 2015年03月, 研究代表者競争的資金
- 日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 京都大学, 2011年04月01日 - 2014年03月31日本研究では「性選択を介した交尾器形態の進化による種分化」のシナリオを検証するために，オオオサムシ亜属を研究対象とし，交尾器形態への性選択の検証と，交尾器形態の種間差をもたらす遺伝子に関する研究を行った．種内で交尾器の機能部分に大きいサイズ変異を示す２集団を用いた交尾実験では，精子競争に関しても雌雄交尾器サイズのマッチングに選択がかかることが分かり，交尾器の長大化に関しては性的対立もしくは異種との繁殖干渉を考慮する必要が示唆された．交尾器形態が大きく異なる姉妹種のトランスクリプトーム比較においては，発現量が種間で異なる遺伝子の中には交尾器形態の種間差に関する有力な候補遺伝子は見出されなかった．
- 日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 京都大学, 2011年04月01日 - 2014年03月31日貝食性オサムシ亜族における巨頭化，狹頭化といった採餌形態の多様化と，陸貝相の対応関係を明らかにするために，中国，韓国，アルメニア，モロッコにおいて野外調査を行った．中国内陸部には，多様な形態の貝食性オサムシ亜族と陸貝（有肺類）が生息するが，巨頭型のオサムシ亜族が生息するのは，小型のオナジマイマイ科陸貝が豊富な場所であった．また，巨頭型・狹頭型のオサムシ亜族が共存する場所では，オナジマイマイ科の種間で著しい形態の多様化がみられ，捕食圧によって，陸貝の適応放散が起こったことが推察された．
- 日本学術振興会, 科学研究費助成事業 基盤研究(C), 基盤研究(C), 首都大学東京, 2009年 - 2011年クモバチ科の原始社会性の進化的起源を明らかにするため,キマダラズアカクモバチのオスの交尾行動および同じ巣場所で活動する個体の間の血縁度を調べた.その結果,オスは長時間(1例として400分)巣口付近で静止し,巣に出入りするメスとの交尾を試みることや,同一巣場所で営巣していた複数メスが父親を共有していたことがわかり,オス個体の交尾の独占が示唆された.また,ルリクモバチのいくつかの形質を精査した結果,オスのみで,体長(前翅縁室の長さ)と腿節の厚さとの間に正のアロメトリーが認められ,発達した腿節は大型オスによる,巣上での交尾の独占を可能にする形質であることが推定された.
- 日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 京都大学, 2008年 - 2010年四国固有のシコクオサムシにおいて,異なる高度環境への局所適応に付随した体サイズ分化が,種分化につながるかどうかを検証した.本種では,標高1000m付近をより低標高では大型個体,高標高では小型個体の集団が存在する.高標高における体長の減少は,高標高における温度と餌資源(ミミズ)の減少に対応している.集団間の体サイズ差は主に遺伝的に決まっている.体サイズの異なる集団間では,体サイズ差による機械的生殖隔離があり,シコクオサムシは高度適応によって種分化しつつあると考えられる.
- 科学研究費補助金／若手研究(B), 2010年, 研究代表者競争的資金
- 科学研究費補助金／若手研究(B), 2008年, 研究代表者競争的資金
- 日本学術振興会, 科学研究費助成事業 特別研究員奨励費, 特別研究員奨励費, 京都大学, 2003年 - 2005年雌雄交尾器における錠と鍵的対応の進化機構雌雄交尾器に見られる錠と鍵的対応の進化過程を解明するため,甲虫目オサムシ科の各種を用いて,以下の研究を行った. 1)交尾器形態と交尾行動の相関した進化 オオオサムシ亜属に特徴的な交尾片は,精子競争における精包の掻き出しに機能している可能性がある.この仮説から予測される交尾片サイズと精包サイズの相関した進化を,22分類群の種間比較解析により検討した.系統的な制約をコントロールするため,核遺伝子5遺伝子座を元にした最尤系統樹を用いて,独立な対比に基づく解析を行った.結果,1)交尾片と精包のサイズは体サイズと比較してそれぞれ3倍および等倍率で進化している;2)交尾片と精包のサイズは正に相関する;3)交尾片と交尾時間は負に相関する,ことが明らかとなった.(論文投稿中) 2)精子競争における交尾器の機能 オサムシ類の精子競争における精包の掻き出し仮説を検証するため,多回交尾下での精包分布と父性の変化を調べた。マイクロサテライト遺伝子を用いた父性解析の結果,80%の確率で精包の掻き出しが起こり,それは掻き出した雄の父性の獲得に寄与していることが明らかとなった(論文投稿中). 3)二型的精子束の進化 精子競争によって生じる性淘汰は,交尾器形態以外にも働き,顕著な形質進化を引き起こし得る.オオオサムシ類で発見された二型的精子束が精子競争による性淘汰の影響を受けているかを明らかにするため,種間比較解析により,交尾器形態や交尾行動との相関した進化の検出を試みた.結果,大小の精子束はそれぞれ著しい多様化を示し,大きな精子束のサイズは交尾片長,交尾後保護時間と正に相関した.これは,著しい精包掻き出しが生じる状況で,大きな精子束が有利になるような性淘汰が働いたことを示唆する(論文準備中).
- 日本学術振興会, 科学研究費助成事業 特別研究員奨励費, 特別研究員奨励費, 東京都立大学, 1999年 - 2000年昆虫の種分化における交尾器の錠と鍵的構造の進化的意義