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佐々木 大介
大学院科学技術イノベーション研究科 科学技術イノベーション専攻
准教授

研究者基本情報

■ 学位
  • 博士（農学）, 東京大学
■ 研究分野
  • ライフサイエンス / 応用微生物学

研究活動情報

■ 論文
  • Kengo Sasaki, Yasunobu Takeshima, Ayami Fujino, Junya Yamashita, Akira Kimoto, Daisuke Sasaki, Akihiko Kondo, Masaya Akashi, Ryo Okumura
    We developed a simulation model of human oral microbiota using Bio Palette oral medium (BPOM) containing 0.02% glucose and lower bacterial nitrogen sources, derived from saliva and dental plaque. By decreasing the concentration of Gifu anaerobic medium (GAM) from 30 to 10 g L-1 , we observed increased ratios of target pathogenic genera, Porphyromonas and Fusobacterium from 0.5% and 1.7% to 1.2% and 3.5%, respectively, in the biofilm on hydroxyapatite (HA) discs. BPOM exhibited the higher ratios of Porphyromonas and Fusobacterium, and amplicon sequence variant number on HA, compared with GAM, modified GAM and basal medium mucin. Mixing glycerol stocks of BPOM culture solutions from four human subjects resulted in comparable ratios of these bacteria to the original saliva. In this simulation model, sitafloxacin showed higher inhibitory effects on P. gingivalis than minocycline hydrochloride at a low dosage of 0.1 μg mL-1 . Probiotics such as Streptococcus salivarius and Limosilactobacillus fermentum also showed significant decreases in Porphyromonas and Fusobacterium ratios on HA, respectively. Overall, the study suggests that BPOM with low carbon and nutrients could be a versatile platform for assessing the efficacy of antibiotics and live biotherapeutics in treating oral diseases caused by Porphyromonas and Fusobacterium.
    2024年04月, Environmental microbiology reports, 16(2) (2), e13243, 英語, 国際誌
    研究論文（学術雑誌）

  • Kentaro Inokuma, Daisuke Sasaki, Kaoru Kurata, Megumi Ichikawa, Yuya Otsuka, Akihiko Kondo
    Abstract Chondroitin sulfate (CS) is a family of glycosaminoglycans and have a wide range of applications in dietary supplements and pharmaceutical drugs. In this study, we evaluated the effects of several types of CS, differing in their sulfated positions, on the human colonic microbiota and their metabolites. CS (CSA, CSC, and CSE) and non-sulfated chondroitin (CH) were added into an in vitro human colonic microbiota model with fecal samples from 10 healthy individuals. CS addition showed a tendency to increase the relative abundance of Bacteroides, Eubacterium, and Faecalibacterium, and CSC and CSE addition significantly increased the total number of eubacteria in the culture of the Kobe University Human Intestinal Microbiota Model. CSE addition also resulted in a significant increase in short-chain fatty acid (SCFA) levels. Furthermore, addition with CSC and CSE increased the levels of a wide range of metabolites including lysine, ornithine, and Ile-Pro-Pro, which could have beneficial effects on the host. However, significant increases in the total number of eubacteria, relative abundance of Bacteroides, and SCFA levels were also observed after addition with CH, and the trends in the effects of CH addition on metabolite concentrations were identical to those of CSC and CSE addition. These results provide novel insight into the contribution of the colonic microbiota to the beneficial effects of dietary CS.
    Springer Science and Business Media LLC, 2023年07月, Scientific Reports, 13(1) (1)
    研究論文（学術雑誌）

  • Daisuke Sasaki, Kengo Sasaki, Aya Abe, Makoto Ozeki, Akihiko Kondo
    Partially hydrolyzed guar gums (PHGGs) are prebiotic soluble dietary fibers. High molecular-weight PHGGs have rapid fermentation and high short-chain fatty acid (SCFA) productivity rates, compared to low molecular-weight PHGGs. Therefore, low molecular-weight PHGGs may have less pronounced prebiotic effects than high molecular-weight PHGGs. However, the effects of PHGGs of different molecular weights on the human intestinal microbiota, as well as their fermentation ability and prebiotic effects, have not been investigated. The aim of this study was to evaluate the effects of two PHGGs of different molecular weights, Sunfiber-R (SF-R; 20 kDa) and Sunfiber-V (SF-V; 5 kDa), on human colonic microbiota and SCFA production. A human intestinal in vitro fermentation model was operated by fecal samples with and without the PHGGs. The addition of 0.2% SF-R or SF-V increased the relative abundance of Bacteroides spp., especially that of Bacteroides uniformis. This increase corresponded to a significant (p = 0.030) and non-significant (p = 0.073) increase in propionate production in response to SF-R and SF-V addition, respectively. Both fibers increased the relative abundance of Faecalibacterium and stimulated an increase in the abundance of unclassified Lachnospiraceae and Bifidobacterium. In conclusion, the low molecular-weight PHGG exerted prebiotic effects on human colonic microbiota to increase SCFA production and bacteria that are beneficial to human health in a manner similar to that of the high molecular-weight forms of PHGG.
    2023年07月, Journal of bioscience and bioengineering, 136(1) (1), 67 - 73, 英語, 国内誌
    研究論文（学術雑誌）

  • Jyumpei Kobayashi, Daisuke Sasaki, Kiyotaka Y Hara, Tomohisa Hasunuma, Akihiko Kondo
    BACKGROUND: Glutathione is a valuable tri-peptide that is industrially produced by fermentation using the yeast Saccharomyces cerevisiae, and is widely used in the pharmaceutical, food, and cosmetic industries. It has been reported that addition of L-serine (L-Ser) is effective at increasing the intracellular glutathione content because L-Ser is the common precursor of L-cysteine (L-Cys) and glycine (Gly) which are substrates for glutathione biosynthesis. Therefore, we tried to enhance the L-Ser biosynthetic pathway in S. cerevisiae for improved glutathione production. RESULTS: The volumetric glutathione production of recombinant strains individually overexpressing SER2, SER1, SER3, and SER33 involved in L-Ser biosynthesis at 48 h cultivation was increased 1.3, 1.4, 1.9, and 1.9-fold, respectively, compared with that of the host GCI strain, which overexpresses genes involved in glutathione biosynthesis. We further examined simultaneous overexpression of SHM2 and/or CYS4 genes involved in Gly and L-Cys biosynthesis, respectively, using recombinant GCI strain overexpressing SER3 and SER33 as hosts. As a result, GCI overexpressing SER3, SHM2, and CYS4 showed the highest volumetric glutathione production (64.0 ± 4.9 mg/L) at 48 h cultivation, and this value is about 2.5-fold higher than that of the control strain. CONCLUSIONS: This study first revealed that engineering of L-Ser and Gly biosynthetic pathway are useful strategies for fermentative glutathione production by S. cerevisiase.
    2022年08月, Microbial cell factories, 21(1) (1), 153 - 153, 英語, 国際誌
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Kengo Sasaki, Daisuke Sasaki, Yota Tsuge, Masahiko Morita, Akihiko Kondo
    Abstract Background It is desirable to improve the anaerobic digestion processes of recalcitrant materials, such as cellulose. Enhancement of methane (CH4) production from organic molecules was previously accomplished through coupling a bioelectrochemical system (BES); however, scaling-up BES-based production is difficult. Here, we developed a two-stage process consisting of a BES using low-cost and low-reactive carbon sheets as the cathode and anode, and a fixed film reactor (FFR) containing conductive material, i.e., carbon fiber textiles (CFTs) (:BES → FFR). By controlling the cathodic current at 2.7 μA/cm2 without abiotic H2 production, the three-electrode BES system was operated to mimic a microbial electrolysis cell. Results The thermophilic BES (inlet pH: 6.1) and FFR (inlet pH: 7.5) were operated using hydraulic retention times (HRTs) of 2.5 and 4.2 days, respectively, corresponding to a cellulose load of 3555.6 mg-carbon (C)/(L day). The BES → FFR process achieved a higher CH4 yield (37.5%) with 52.8 vol% CH4 in the product gas compared to the non-bioelectrochemical system (NBES) → FFR process, which showed a CH4 yield of 22.1% with 46.8 vol% CH4. The CH4 production rate (67.5 mM/day) obtained with the BER → FFR process was much higher than that obtained using electrochemical methanogenesis (0.27 mM/day). Application of the electrochemical system or CFTs improved the yields of CH4 with the NBES → FFR or BES → non-fixed film reactor process, respectively. Meta 16S rRNA sequencing revealed that putative cellulolytic bacteria (identified as Clostridium species) were present in the BES and NBES, and followed (BES→ and NBES→) FFR. Notably, H2-consuming methanogens, Methanobacterium sp. and Methanosarcina sp., showed increased relative abundances in the suspended fraction and attached fraction of (BES→) FFR, respectively, compared to that of (NBES→) FFR, although these methanogens were observed at trace levels in the BES and NBES. Conclusions These results indicate that bioelectrochemical preprocessing at a low current effectively induces interspecies H2 transfer in the FFR with conductive material. Sufficient electrochemical preprocessing was observed using a relatively short HRT. This type of two-stage process, BES → FFR, is useful for stabilization and improvement of the biogas (CH4) production from cellulosic material, and our results imply that the two-stage system developed here may be useful with other recalcitrant materials.
    Springer Science and Business Media LLC, 2021年12月, Biotechnology for Biofuels, 14(1) (1)
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Kengo Sasaki, Daisuke Sasaki, Katsunori Sasaki, Yuto Nishidono, Akihiro Yamamori, Ken Tanaka, Akihiko Kondo
    AbstractDaikenchuto (DKT) is a Japanese traditional herbal (Kampo) medicine containing ginseng, processed ginger, and Japanese or Chinese pepper. We aimed to determine how DKT affects human colonic microbiota. An in vitro microbiota model was established using fecal inocula collected from nine healthy volunteers, and each model was found to retain operational taxonomic units similar to the ones in the original human fecal samples. DKT was added to the in vitro microbiota model culture at a concentration of 0.5% by weight. Next-generation sequencing of bacterial 16S rRNA gene revealed a significant increase in the relative abundance of bacteria related to the Bifidobacterium genus in the model after incubation with DKT. In pure cultures, DKT significantly promoted the growth of Bifidobacterium adolescentis, but not that of Fusobacterium nucleatum or Escherichia coli. Additionally, in pure cultures, B. adolescentis transformed ginsenoside Rc to Rd, which was then probably utilized for its growth. Our study reveals the in vitro bifidogenic effect of DKT that likely contributes to its beneficial effects on the human colon.
    Springer Science and Business Media LLC, 2021年12月, Scientific Reports, 11(1) (1), 英語
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Naofumi Yoshida, Tomoya Yamashita, Tatsunori Osone, Tetsuya Hosooka, Masakazu Shinohara, Seiichi Kitahama, Kengo Sasaki, Daisuke Sasaki, Takeshi Yoneshiro, Tomohiro Suzuki, Takuo Emoto, Yoshihiro Saito, Genki Ozawa, Yushi Hirota, Yasuyuki Kitaura, Yoshiharu Shimomura, Yuko Okamatsu-Ogura, Masayuki Saito, Akihiko Kondo, Shingo Kajimura, Takeshi Inagaki, Wataru Ogawa, Takuji Yamada, Ken-ichi Hirata
    The gut microbiome has emerged as a key regulator of obesity; however, its role in brown adipose tissue (BAT) metabolism and association with obesity remain to be elucidated. We found that the levels of circulating branched-chain amino acids (BCAA) and their cognate α-ketoacids (BCKA) were significantly correlated with the body weight in humans and mice and that BCAA catabolic defects in BAT were associated with obesity in diet-induced obesity (DIO) mice. Pharmacological systemic enhancement of BCAA catabolic activity reduced plasma BCAA and BCKA levels and protected against obesity; these effects were reduced in BATectomized mice. DIO mice gavaged with Bacteroides dorei and Bacteroides vulgatus exhibited improved BAT BCAA catabolism and attenuated body weight gain, which were not observed in BATectomized DIO mice. Our data have highlighted a possible link between the gut microbiota and BAT BCAA catabolism and suggest that Bacteroides probiotics could be used for treating obesity.
    Elsevier BV, 2021年11月, iScience, 24(11) (11), 103342 - 103342, 英語, 国際誌
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Namiko Hoshi, Jun Inoue, Daisuke Sasaki, Kengo Sasaki
    The human gut harbors a complex microbial community that performs a range of metabolic, physiological, and immunological functions. The host and its inhabiting microorganisms are often referred to as a "superorganism." Dysbiosis of gut microflora has been associated with the pathogenesis of intestinal disorders including inflammatory bowel disease, colorectal cancer, and extra-intestinal disorders such as cardiovascular disease. Therefore, gut microbiome interventions are important for the prevention and treatment of diseases. However, ethical, economic, scientific, and time constraints limit the outcome of human intervention or animal studies targeting gut microbiota. We recently developed an in vitro batch fermentation model (the Kobe University Human Intestinal Microbiota Model, KUHIMM) that is capable of hosting a majority of gut microbial species in humans and also detects the metabolites produced by microorganisms in real time. In this mini review, we elucidated the characteristics of the KUHIMM and its applicability in analyzing the effect of diet, drugs, probiotics, and prebiotics on intestinal bacteria. In addition, we introduce as examples its application to disease models, such as ulcerative colitis, in which intestinal bacteria are intricately involved in the process of pathogenesis. We also discuss the potential of the KUHIMM in precision medicine. KEY POINTS: • In vitro gut fermentation model to simulate human colonic microbiota • Screening of potential prebiotics and probiotic candidates in healthy model • Construction of disease models of ulcerative colitis and coronary artery disease.
    Springer Science and Business Media LLC, 2021年04月, Applied Microbiology and Biotechnology, 105(7) (7), 2625 - 2632, 英語, 国際誌
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Kengo Sasaki, Tomoyuki Mori, Namiko Hoshi, Daisuke Sasaki, Jun Inoue, Reiko Shinkura, Akihiko Kondo
    Abstract W27 monoclonal immunoglobulin A (IgA) suppresses pathogenic Escherichia coli cell growth; however its effect on the human intestine remains unclear. We thus aimed to determine how W27 IgA affects the human colonic microbiota using the in vitro microbiota model. This model was established using fecal samples collected from 12 healthy volunteers; after anaerobic cultivation, each model was found to retain the genera found in the original human fecal samples. After pre-incubating W27 IgA with the respective fecal sample in aerobic condition, the mixture of W27 IgA (final concentration, 0.5 µg/mL) and each fecal sample was added to the in vitro microbiota model and cultured under anaerobic condition,. Next-generation sequencing of the bacterial 16S rRNA gene revealed that W27 IgA addition significantly decreased the relative abundance of bacteria related to the genus Escherichia in the model. Additionally, at a final concentration of 5 µg/mL, W27 IgA delayed growth in pure culture of Escherichia coli isolated from human fecal samples. Our study thus revealed the suppressive effect of W27 IgA on the genus Escherichia at relatively low-concentrations and the usefulness of an in vitro microbiota model to evaluate the effect of IgA as a gut microbiota regulator.
    Research Square, 2021年03月, 英語
    神戸大学リポジトリ（Kernel）へのリンク

  • Ayaka Nakashima, Kengo Sasaki, Daisuke Sasaki, Kosuke Yasuda, Kengo Suzuki, Akihiko Kondo
    AbstractThe alga Euglena gracilis (E. gracilis) has recently gained attention as a health food, but its effects on human gut microbiota remain unknown. This study aimed to determine the effect of E. gracilis on gut microbiota and defecation due to modulation of microbiota composition in vitro and in vivo. The in vitro model simulating human colonic microbiota revealed that E. gracilis addition stimulated the growth of commensal Faecalibacterium. Further, E. gracilis addition enhanced butyrate production by Faecalibacterium prausnitzii. Paramylon, an insoluble dietary fibre that accumulates in E. gracilis and is the main component of E. gracilis, did not stimulate Faecalibacterium growth in vitro. Daily ingestion of 2 g of E. gracilis for 30 days increased bowel movement frequency as well as stool volume in 28 human participants. Collectively, these findings indicate that E. gracilis components other than paramylon, stimulate the growth of Faecalibacterium to improve digestive health as well as promote defecation by increasing butyrate production.
    Springer Science and Business Media LLC, 2021年01月, Scientific Reports, 11(1) (1), 英語
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Naofumi Yoshida, Tomoya Yamashita, Shigenobu Kishino, Hikaru Watanabe, Kengo Sasaki, Daisuke Sasaki, Tokiko Tabata, Yuta Sugiyama, Nahoko Kitamura, Yoshihiro Saito, Takuo Emoto, Tomohiro Hayashi, Tomoya Takahashi, Masakazu Shinohara, Ro Osawa, Akihiko Kondo, Takuji Yamada, Jun Ogawa, Ken-ichi Hirata
    Springer Science and Business Media LLC, 2020年12月, Scientific Reports, 10(1) (1), 13009, 英語
    [査読有り]
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Kengo Sasaki, Daisuke Sasaki, Asuka Hannya, Jun Tsubota, Akihiko Kondo
    Springer Science and Business Media LLC, 2020年12月, Scientific Reports, 10(1) (1), 8516, 英語
    [査読有り]
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Shunsuke Oba, Tadahiro Sunagawa, Reiko Tanihiro, Kyoko Awashima, Hiroshi Sugiyama, Tetsuji Odani, Yasunori Nakamura, Akihiko Kondo, Daisuke Sasaki, Kengo Sasaki
    Abstract Yeast mannan (YM) is an indigestible water-soluble polysaccharide of the yeast cell wall, with a notable prebiotic effect on the intestinal microbiota. We previously reported that YM increased Bacteroides thetaiotaomicron abundance in in vitro rat faeces fermentation, concluding that its effects on human colonic microbiota should be investigated. In this study, we show the effects of YM on human colonic microbiota and its metabolites using an in vitro human faeces fermentation system. Bacterial 16S rRNA gene sequence analysis showed that YM administration did not change the microbial diversity or composition. Quantitative real-time PCR analysis revealed that YM administration significantly increased the relative abundance of Bacteroides ovatus and B. thetaiotaomicron. Moreover, a positive correlation was observed between the relative ratio (with or without YM administration) of B. thetaiotaomicron and B. ovatus (r = 0.92), suggesting that these bacteria utilise YM in a coordinated manner. In addition, YM administration increased the production of acetate, propionate, and total short-chain fatty acids. These results demonstrate the potential of YM as a novel prebiotic that selectively increases B. thetaiotaomicron and B. ovatus and improves the intestinal environment. The findings also provide insights that might be useful for the development of novel functional foods.
    Springer Science and Business Media LLC, 2020年10月, Scientific Reports, 10(1) (1), 17351, 英語
    研究論文（学術雑誌）

  • Katsuki Murai, Daisuke Sasaki, Shunsuke Kobayashi, Akira Yamaguchi, Hiroto Uchikura, Tomokazu Shirai, Kengo Sasaki, Akihiko Kondo, Yota Tsuge
    Glucose is metabolized through central metabolic pathways such as glycolysis and the pentose phosphate pathway (PPP) to synthesize downstream metabolites including amino acids. However, how the split ratio of carbon flux between glycolysis and PPP specifically affects the formation of downstream metabolites remains largely unclear. Here, we conducted a comprehensive metabolomic analysis to investigate the effect of the split ratio between glycolysis and the PPP on the intracellular concentration of amino acids and their derivatives in Corynebacterium glutamicum. The split ratio was varied by exchanging the promoter of a gene encoding glucose 6-phosphate isomerase (PGI). The ratio was correlated with the pgi transcription level and the enzyme activity. Concentrations of threonine and lysine-derivative 1,5-diaminopentane increased with an increase of the split ratio into the PPP. In contrast, concentrations of alanine, leucine, and valine were increased with an increase of the split ratio into glycolysis. These results could provide a new engineering target for improving the production of the amino acids and the derivatives.
    2020年07月, ACS synthetic biology, 9(7) (7), 1615 - 1622, 英語, 国際誌
    [査読有り]
    研究論文（学術雑誌）

  • Effective bifidogenic growth factors cyclo-Val-Leu and cyclo-Val-Ile produced by Bacillus subtilis C-3102 in the human colonic microbiota model
    Misaki Hatanaka, Hiroto Morita, Yumi Aoyagi, Kengo Sasaki, Daisuke Sasaki, Akihiko Kondo, Teppei Nakamura
    2020年05月, Scientific Reports, 10, 7591, 英語
    [査読有り]
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Kengo Sasaki, Daisuke Sasaki, Jun Inoue, Namiko Hoshi, Takayuki Maeda, Ryouichi Yamada, Akihiko Kondo
    The aim of this study was to clarify the effect of the spore-forming and lactic acid-producing probiotic strain, Bacillus coagulans SANK 70258, on human colonic microbiota of healthy subjects and ulcerative colitis patients. A model culture system was employed to construct the in vitro human colonic microbiota, to retain the bacterial species richness and simulate the patient's disordered composition, from the fecal inoculum. Bacterial 16S rRNA gene sequencing confirmed that administration of B. coagulans SANK 70258 (at an initial concentration of 4 × 107-total cells/mL) suppressed bacteria related to the family Enterobacteriaceae in the microbiota models for both healthy subjects (P = 0.016) and ulcerative colitis patients (P = 0.023). In addition, administration of B. coagulans SANK 70258 increased bacteria related to the family Lachnospiraceae (P = 0.031), thereby enhancing butyrate production (P = 0.031) in the microbiota models of healthy subjects. However, these changes were not observed in the microbiota models of ulcerative colitis patients, likely owing to the low abundance of Lachnospiraceae species. This study demonstrates the potential of B. coagulans SANK 70258 to exhibit antimicrobial activity against harmful organisms in patients with ulcerative colitis, while improving the intestinal microenvironment by increasing butyrogenesis in healthy persons. KEY POINTS: • B. coagulans SANK 70258 treatment reduced colonic Enterobacteriaceae species. • B. coagulans SANK 70258 treatment enhanced butyrogenesis in healthy individuals. • B. coagulans SANK 70258 treatment increased Lachnospiraceae in healthy persons. • B. coagulans SANK 70258 improves the colonic microenvironment in ulcerative colitis.
    2020年03月, Applied microbiology and biotechnology, 英語, 国際誌
    [査読有り]
    神戸大学リポジトリ（Kernel）へのリンク

  • Bifidogenic and butyrogenic effects of young barely leaf extract in an in vitro human colonic microbiota model
    Daisuke Sasaki, Kengo Sasaki, Yasushi Kadowaki, Yasuyuki Aotsuka, Akihiko Kondo
    2019年11月, AMB Express, 9, 182, 英語
    [査読有り]
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Ryohei Shinohara, Kengo Sasaki, Jun Inoue, Namiko Hoshi, Itsuko Fukuda, Daisuke Sasaki, Akihiko Kondo, Ro Osawa
    Microbial production of butyrate is impaired in patients with ulcerative colitis (UC); however, this inhibition is not well understood in Japanese UC patients. Therefore, we quantitatively analyzed genes encoding butyryl-CoA:acetate CoA-transferase (but) and butyrate kinase (buk) in the gut microbiota of Japanese patients with UC and healthy volunteers (HVs). But showed higher levels than buk. Moreover, patients with UC showed significantly decreased levels of but associated with Roseburia sp./Eubacterium rectale compared with HVs. But, which is associated with Faecalibacterium sp., was maintained in patients with UC, with an unchanged relative abundance of Faecalibacterium sp. microorganisms in patients with UC compared with HVs.
    2019年10月, Bioscience of Microbiota, Food and Health, 38(4) (4), 159 - 163, 英語, 国内誌
    [査読有り]
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Naofumi Yoshida, Kengo Sasaki, Daisuke Sasaki, Tomoya Yamashita, Hajime Fukuda, Tomohiro Hayashi, Tokiko Tabata, Ro Osawa, Ken-Ichi Hirata, Akihiko Kondo
    AIM: Bacteroides vulgatus and B. dorei have a protective effect against atherosclerosis, suggesting that expansion of these species in the gut microbiota could help patients with coronary artery disease (CAD). This study aimed to investigate the effect of resistant starch (RS) on the gut microbiota and its metabolites in fecal sample cultures from patients with CAD and individuals without CAD, using a single-batch fermentation system. METHODS: Fecal samples from 11 patients with CAD and 10 individuals without CAD were fermented for 30 h with or without RS in the Kobe University Human Intestinal Microbiota Model (KUHIMM). Gut microbiota and the abundance of B. vulgatus and B. dorei were analyzed using 16S ribosomal ribonucleic acid (rRNA) gene sequencing and the quantitative polymerase chain reaction. Short-chain fatty acids were analyzed using high-performance liquid chromatography. RESULTS: Gut microbial analysis showed significantly lower levels of B. vulgatus and B. dorei in the original fecal samples from patients with CAD, which was simulated after 30 h of fermentation in the KUHIMM. Although RS significantly increased the absolute numbers of B. vulgatus and B. dorei, and butyrate levels in CAD fecal sample cultures, the numbers varied among each patient. CONCLUSIONS: The effect of RS on gut microbiota and its metabolites in the KUHIMM varied between CAD and non-CAD fecal sample cultures. The KUHIMM may be useful for preclinical evaluations of the effects of RS on the gut microbiota and its metabolites.
    2019年08月, Journal of atherosclerosis and thrombosis, 26(8) (8), 705 - 719, 英語, 国内誌
    [査読有り]
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Kengo Sasaki, Jun Inoue, Daisuke Sasaki, Namiko Hoshi, Tomokazu Shirai, Itsuko Fukuda, Takeshi Azuma, Akihiko Kondo, Ro Osawa
    Compositional alteration of the gut microbiota is associated with ulcerative colitis (UC). Here, a model culture system is established for the in vitro human colonic microbiota of UC, which will be helpful for determining medical interventions. 16S ribosomal RNA sequencing confirms that UC models are successfully developed from fecal inoculum and retain the bacterial species biodiversity of UC feces. The UC models closely reproduce the microbial components and successfully preserve distinct clusters from the healthy subjects (HS), as observed in the feces. The relative abundance of bacteria belonging to the family Lachnospiraceae significantly decreases in the UC models compared to that in HS, as observed in the feces. The system detects significantly lower butyrogenesis in the UC models than that in HS, correlating with the decreased abundance of Lachnospiraceae. Interestingly, the relative abundance of Lachnospiraceae does not correlate with disease activity (defined as partial Mayo score), suggesting that Lachnospiraceae persists in UC patients at a decreased level, irrespective of the alteration in disease activity. Moreover, the system shows that administration of Clostridium butyricum MIYAIRI restores butyrogenesis in the UC model. Hence, the model detects deregulation in the intestinal environment in UC patients and may be useful for simulating the effect of probiotics.
    2019年05月, Biotechnology Journal, 14(5) (5), 1800555, 英語, 国際誌
    [査読有り]
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Sustainable production of glutathione from lignocellulose-derived sugars using engineered Saccharomyces cerevisiae
    KOBAYASHI Jyumpei, SASAKI Daisuke, BAMBA Takahiro, HASUNUMA Tomohisa, KONDO Akihiko
    2019年02月, Applied Microbiology and Biotechnology, 103(3) (3), 1243 - 1254, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Daisuke Sasaki, Kengo Sasaki, Yota Tsuge, Akihiko Kondo
    Background: Using a microbial fuel cell (MFC), we observed that a complex microbial community decomposed starch and transferred electrons to a graphite felt anode to generate current. In spite of the same reactor configuration, inoculum, substrate, temperature, and pH, MFCs produced different current and power density. To understand which factor(s) affected electricity generation, here, we analyzed a complex microbial community in an anodic biofilm and fermentation broth using Illumina MiSeq sequencing and metabolomics. Results: Microbial biomass on the anode was lower in MFCs generating more electricity (0.09-0.16 mg cm-2-anode) than in those generating less electricity (0.60-2.80 mg cm-2-anode), while being equal (3890-4196 mg L-1-broth) in the fermentation broth over the same operational period. Chemical oxygen demand removal and acetate concentration were also similar in fermentation broths. MFCs generating more electricity had relatively more exoelectrogenic bacteria, such as Geobacter sp., but fewer acetate-utilizing Methanosarcina sp. and/or Lactococcus sp. in anodic biofilms. Accordingly, anodic biofilms generating more electricity presented higher levels of most intracellular metabolites related to the tricarboxylic acid cycle and a higher intracellular ATP/ADP ratio, but a lower intracellular NADH/NAD+ ratio. Moreover, the level of intracellular glutamate, an essential metabolite for microbial anabolic reactions, correlated negatively with current density. Conclusion: Microbial growth on the anode and intracellular glutamate levels negatively affect electricity generation by MFCs. Reduced formation of anodic biofilm, in which intracellular glutamate concentration is 33.9 μmol g-cell-1 or less, favors the growth of acetate-utilizing Geobacter sp. on the anode and improves current generation.
    2019年, Biotechnology for biofuels, 12, 72 - 72, 英語, 国際誌
    [査読有り]
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Widespread effect of N-acetyl-D-glucosamine assimilation on the metabolisms of amino acids, purines, and pyrimidines in Scheffersomyces stipitis
    INOKUMA Kentaro, MATSUDA Mami, SASAKI Daisuke, HASUNUMA Tomohisa, KONDO Akihiko
    2018年09月, Microbial Cell Factories, 17, 153, 英語
    [査読有り]
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • A procedure for precise determination of glutathione produced by Saccharomyces cerevisiae
    KOBAYASHI Jyumpei, SASAKI Daisuke, KONDO Akihiko
    2018年06月, Bio-protocol, 8(12) (12), e2887, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Daisuke Sasaki, Kazuhide Kamiya, Shuji Nakanishi, Akihiko Kondo, Souichiro Kato
    Microbial electrochemical systems (MESs) are expected to be put into practical use as an environmental technology that can support a future environmentally friendly society. However, conventional MESs present a challenge of inevitably increasing initial investment, mainly due to requirements for a large numbers of electrode assemblies. In this review, we introduce electrochemical biotechnologies that are under development and can minimize the required electrode assemblies. The novel biotechnologies, called electro-fermentation and indirect electro-stimulation, can drive specific microbial metabolism by electrochemically controlling intercellular and extracellular redox states, respectively. Other technologies, namely electric syntrophy and microbial photo-electrosynthesis, obviate the need for electrode assemblies, instead stimulating targeted reactions by using conductive particles to create new metabolic electron flows.
    2018年04月, Current opinion in biotechnology, 50, 182 - 188, 英語, 国際誌
    [査読有り]
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure
    Daisuke Sasaki, Kengo Sasaki, Naoko Ikuta, Takahiro Yasuda, Itsuko Fukuda, Akihiko Kondo, Ro Osawa
    2018年01月, Scientific Reports, 8, 435, 英語
    [査読有り]
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Kengo Sasaki, Daisuke Sasaki, Yota Tsuge, Masahiko Morita, Akihiko Kondo
    Background: Bioelectrochemical systems (BESs) are an innovative technology developed to influence conventional anaerobic digestion. We examined the feasibility of applying a BES to dark hydrogen fermentation and its effects on a two-stage fermentation process comprising hydrogen and methane production. The BES used low-cost, low-reactivity carbon sheets as the cathode and anode, and the cathodic potential was controlled at - 1.0 V (vs. Ag/AgCl) with a potentiostat. The operation used 10 g/L glucose as the major carbon source. Results: The electric current density was low throughout (0.30-0.88 A/m2 per electrode corresponding to 0.5-1.5 mM/day of hydrogen production) and water electrolysis was prevented. At a hydraulic retention time of 2 days with a substrate pH of 6.5, the BES decreased gas production (hydrogen and carbon dioxide contents: 52.1 and 47.1%, respectively), compared to the non-bioelectrochemical system (NBES), although they had similar gas compositions. In addition, a methane fermenter (MF) was applied after the BES, which increased gas production (methane and carbon dioxide contents: 85.1 and 14.9%, respectively) compared to the case when the MF was applied after the NBES. Meta 16S rRNA sequencing revealed that the BES accelerated the growth of Ruminococcus sp. and Veillonellaceae sp. and decreased Clostridium sp. and Thermoanaerobacterium sp., resulting in increased propionate and ethanol generation and decreased butyrate generation; however, unknowingly, acetate generation was increased in the BES. Conclusions: The altered redox potential in the BES likely transformed the structure of the microbial consortium and metabolic pattern to increase methane production and decrease carbon dioxide production in the two-stage process. This study showed the utility of the BES to act on the microbial consortium, resulting in improved gas production from carbohydrate compounds.
    2018年, Biotechnology for biofuels, 11, 173 - 173, 英語, 国際誌
    [査読有り]
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Kiyotaka Y. Hara, Jyumpei Kobayashi, Ryosuke Yamada, Daisuke Sasaki, Yuki Kuriya, Yoko Hirono-Hara, Jun Ishii, Michihiro Araki, Akihiko Kondo
    2017年11月, FEMS YEAST RESEARCH, 17(7) (7), fox061, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Yota Tsuge, Hideo Kawaguchi, Masahiro Yasukawa, Daisuke Sasaki, Takashi Sazuka, Eiji Kamio, Chiaki Ogino, Hideto Matsuyama, Akihiko Kondo
    The juice from sweet sorghum cultivar SIL-05 (harvested at physiological maturity) was extracted, and the component sucrose and reducing sugars (such as glucose and fructose) were subjected to a membrane separation process to purify the sucrose for subsequent sugar refining and to obtain a feedstock for repeated bioethanol production. Nanofiltration (NF) of an ultrafiltration (UF) permeate using an NTR-7450 membrane (Nitto Denko Corporation, Osaka, Japan) concentrated the juice and produced a sucrose-rich fraction (143.2 g L-1 sucrose, 8.5 g L-1 glucose, and 4.5 g L-1 fructose). In addition, the above NF permeate was concentrated using an ESNA3 NF membrane to provide concentrated permeated sugars (227.9 g L-1) and capture various amino acids in the juice, enabling subsequent ethanol fermentation without the addition of an exogenous nitrogen source. Sequential batch fermentation using the ESNA3 membrane concentrate provided an ethanol titer and theoretical ethanol yield of 102.5-109.5 g L-1 and 84.4-89.6%, respectively, throughout the five-cycle batch fermentation by Saccharomyces cerevisiae BY4741. Our results demonstrate that a membrane process using UF and two types of NF membranes has the potential to allow sucrose purification and repeated bioethanol production.
    2017年08月, Applied microbiology and biotechnology, 101(15) (15), 6007 - 6014, 英語, 国際誌
    [査読有り]
    研究論文（学術雑誌）

  • Jyumpei Kobayashi, Daisuke Sasaki, Kiyotaka Y. Hara, Tomohisa Hasunuma, Akihiko Kondo
    2017年03月, MICROBIAL CELL FACTORIES, 16, 44, 英語
    [査読有り]
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Kengo Sasaki, Daisuke Sasaki, Naoko Okai, Kosei Tanaka, Ryohei Nomoto, Itsuko Fukuda, Ken-Ichi Yoshida, Akihiko Kondo, Ro Osawa
    Accumulating evidence suggests that dietary taurine (2-aminoethanesulfonic acid) exerts beneficial anti-inflammatory effects in the large intestine. In this study, we investigated the possible impact of taurine on human colonic microbiota using our single-batch fermentation system (Kobe University Human Intestinal Microbiota Model; KUHIMM). Fecal samples from eight humans were individually cultivated with and without taurine in the KUHIMM. The results showed that taurine remained largely undegraded after 30 h of culturing in the absence of oxygen, although some 83% of the taurine was degraded after 30 h of culturing under aerobic conditions. Diversity in bacterial species in the cultures was analyzed by 16S rRNA gene sequencing, revealing that taurine caused no significant change in the diversity of the microbiota; both operational taxonomic unit and Shannon-Wiener index of the cultures were comparable to those of the respective source fecal samples. In addition, principal coordinate analysis indicated that taurine did not alter the composition of bacterial species, since the 16S rRNA gene profile of bacterial species in the original fecal sample was maintained in each of the cultures with and without taurine. Furthermore, metabolomic analysis revealed that taurine did not affect the composition of short-chain fatty acids produced in the cultures. These results, under these controlled but artificial conditions, suggested that the beneficial anti-inflammatory effects of dietary taurine in the large intestine are independent of the intestinal microbiota. We infer that dietary taurine may act directly in the large intestine to exert anti-inflammatory effects.
    2017年, PloS one, 12(7) (7), e0180991, 英語, 国際誌
    [査読有り]
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Daisuke Sasaki, Kengo Sasaki, Yota Tsuge, Akihiko Kondo
    2016年12月, AMB EXPRESS, 6, 125, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Mami Okamoto, Tomokazu Shirai, Yota Tsuge, Ayami Fujino, Daisuke Sasaki, Masahiko Morita, Fumio Matsuda, Jun Kikuchi, Akihiko Kondo
    2016年09月, BIORESOURCE TECHNOLOGY, 216, 830 - 837, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Keisuke Yamamoto, Kiyotaka Y. Hara, Toshihiko Morita, Akira Nishimura, Daisuke Sasaki, Jun Ishii, Chiaki Ogino, Noriyuki Kizaki, Akihiko Kondo
    2016年09月, MICROBIAL CELL FACTORIES, 15(1) (1), 155, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Risa Takagi, Kengo Sasaki, Daisuke Sasaki, Itsuko Fukuda, Kosei Tanaka, Ken-ichi Yoshida, Akihiko Kondo, Ro Osawa
    2016年08月, PLOS ONE, 11(8) (8), e0160533, 英語
    [査読有り]
    研究論文（学術雑誌）
    神戸大学リポジトリ（Kernel）へのリンク

  • Jieun Song, Daisuke Sasaki, Kengo Sasaki, Souichiro Kato, Akihiko Kondo, Kazuhito Hashimoto, Shuji Nakanishi
    2016年01月, PROCESS BIOCHEMISTRY, 51(1) (1), 34 - 38, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo
    2015年06月, BIORESOURCE TECHNOLOGY, 186, 351 - 355, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Mami Okamoto, Tomokazu Shirai, Yota Tsuge, Hiroshi Teramura, Daisuke Sasaki, Hideo Kawaguchi, Tomohisa Hasunuma, Chiaki Ogino, Fumio Matsuda, Jun Kikuchi, Akihiko Kondo
    2015年06月, BIOTECHNOLOGY FOR BIOFUELS, 8, 88, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Hiroshi Teramura, Kentaro Inokuma, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo
    2015年06月, BIORESOURCE TECHNOLOGY, 185, 263 - 268, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Tomoyuki Hori, Shin Haruta, Daisuke Sasaki, Dai Hanajima, Yoshiyuki Ueno, Atsushi Ogata, Masaharu Ishii, Yasuo Igarashi
    2015年03月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 119(3) (3), 337 - 344, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Daisuke Sasaki, Kengo Sasaki, Yota Tsuge, Masahiko Morita, Akihiko Kondo
    2014年11月, BIORESOURCE TECHNOLOGY, 172, 83 - 90, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Tomohisa Hasunuma, Takatoshi Sakamoto, Yuri Sakihama, Chiaki Ogino, Akihiko Kondo
    2014年10月, BIORESOURCE TECHNOLOGY, 169, 380 - 386, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Hiroshi Teramura, Satoshi Wakai, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Akihiko Kondo
    2014年10月, BIORESOURCE TECHNOLOGY, 169, 821 - 825, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Akihiko Kondo
    2014年05月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 117(5) (5), 598 - 601, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Daisuke Sasaki, Yuri Sakihama, Hiroshi Teramura, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo
    2013年11月, BIORESOURCE TECHNOLOGY, 147, 84 - 88, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Masahiko Morita, Daisuke Sasaki, Naoya Ohmura, Yasuo Igarashi
    2013年08月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(15) (15), 7005 - 7013, 英語
    [査読有り]
    研究論文（学術雑誌）

  • 電気化学システムを用いた模擬生ごみからの水素発酵の制御
    佐々木 建吾, 佐々木 大介, 森田 仁彦, 五十嵐 泰夫, 近藤 昭彦
    水素エネルギー協会, 2013年03月, 水素エネルギーシステム, 38(1) (1), 33 - 37, 日本語
    研究論文（学術雑誌）

  • Daisuke Sasaki, Kengo Sasaki, Atsushi Watanabe, Masahiko Morita, Norio Matsumoto, Yasuo Igarashi, Naoya Ohmura
    Elsevier Ltd, 2013年, Bioresource Technology, 129, 366 - 373, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Daisuke Sasaki, Kengo Sasaki, Atsushi Watanabe, Masahiko Morita, Yasuo Igarashi, Naoya Ohmura
    Springer Verlag, 2013年, AMB Express, 3(17) (17), 1 - 9, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Masahiko Morita, Daisuke Sasaki, Norio Matsumoto, Naoya Ohmura, Yasuo Igarashi
    2012年10月, BIOCHEMICAL ENGINEERING JOURNAL, 68, 104 - 108, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Daisuke Sasaki, Masahiko Morita, Kengo Sasaki, Atsushi Watanabe, Naoya Ohmura
    2012年10月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 114(4) (4), 435 - 439, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Masahiko Morita, Norio Matsumoto, Daisuke Sasaki, Shin-ichi Hirano, Naoya Ohmura, Yasuo Igarashi
    2012年07月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 114(1) (1), 64 - 69, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Daisuke Sasaki, Kengo Sasaki, Masahiko Morita, Shin-ichi Hirano, Norio Matsumoto, Naoya Ohmura
    2012年07月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 114(1) (1), 59 - 63, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Masahiko Morita, Daisuke Sasaki, Jun Nagaoka, Norio Matsumoto, Naoya Ohmura, Hiraku Shinozaki
    2011年11月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 112(5) (5), 469 - 472, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Tomoyuki Hori, Daisuke Sasaki, Shin Haruta, Toru Shigematsu, Yoshiyuki Ueno, Masaharu Ishii, Yasuo Lgarashi
    2011年07月, MICROBIOLOGY-SGM, 157, 1980 - 1989, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Masahiko Morita, Daisuke Sasaki, Shin-ichi Hirano, Norio Matsumoto, Atsushi Watanabe, Naoya Ohmura, Yasuo Igarashi
    2011年07月, BIORESOURCE TECHNOLOGY, 102(13) (13), 6837 - 6842, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Daisuke Sasaki, Tomoyuki Hori, Shin Haruta, Yoshiyuki Ueno, Masaharu Ishii, Yasuo Igarashi
    2011年01月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 111(1) (1), 41 - 46, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Masahiko Morita, Daisuke Sasaki, Shin-ichi Hirano, Norio Matsumoto, Naoya Ohmura, Yasuo Igarashi
    2011年01月, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 111(1) (1), 47 - 49, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Shin-ichi Hirano, Masahiko Morita, Daisuke Sasaki, Norio Matsumoto, Naoya Ohmura, Yasuo Igarashi
    2011年01月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 89(2) (2), 449 - 455, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Masahiko Morita, Shin-ichi Hirano, Daisuke Sasaki, Naoya Ohmura, Yasuo Igarashi
    2010年07月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(4) (4), 1579 - 1586, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Daisuke Sasaki, Masahiko Morita, Shin-ichi Hirano, Norio Matsumoto, Naoya Ohmura, Yasuo Igarashi
    2010年05月, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 86(5) (5), 1573 - 1583, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Kengo Sasaki, Daisuke Sasaki, Masahiko Morita, Shin-ichi Hirano, Norio Matsumoto, Naoya Ohmura, Yasuo Igarashi
    2010年05月, BIORESOURCE TECHNOLOGY, 101(10) (10), 3415 - 3422, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Y. Ueno, D. Sasaki, H. Fukui, S. Haruta, M. Ishii, Y. Igarashi
    2006年08月, JOURNAL OF APPLIED MICROBIOLOGY, 101(2) (2), 331 - 343, 英語
    [査読有り]
    研究論文（学術雑誌）

  • Y Okuda, D Sasaki, S Nogami, Y Kaneko, Y Ohya, Y Anraku
    2003年05月, YEAST, 20(7) (7), 563 - 573, 英語
    [査読有り]
    研究論文（学術雑誌）

■ MISC
  • 電気化学的前処理／通電式担体充填による2段発酵を廃水・廃棄物処理へ適用
    佐々木建吾, 佐々木大介, 近藤昭彦
    2021年05月, 化学と生物, 59(12) (12), 247 - 253, 日本語

  • ヒト腸内細菌叢モデルによるプレバイオティクスの評価
    佐々木 大介, 佐々木 建吾
    2019年08月, アグリバイオ, 3(9) (9), 53 - 58, 日本語
    記事・総説・解説・論説等（学術雑誌）

  • 日本人潰瘍性大腸炎患者の腸内細菌叢における2種の酪酸産生遺伝子の定量
    篠原 涼平, 佐々木 建吾, 井上 潤, 星 奈美子, 福田 伊津子, 佐々木 大介, 近藤 昭彦, 大澤 朗
    (公財)日本ビフィズス菌センター, 2019年04月, 腸内細菌学雑誌, 33(2) (2), 105 - 105, 日本語

  • in viro培養システムによる食物繊維のヒト腸管細菌叢への影響評価
    佐々木 大介, 佐々木 建吾, 近藤 昭彦, 大澤 朗
    2018年12月, ルミナコイド研究（日本食物繊維学会誌）, 22(2) (2), 63 - 74, 日本語
    記事・総説・解説・論説等（学術雑誌）

  • 培養系ヒト腸内細菌叢モデルKUHIMMを利用した食物繊維の影響評価
    生田 直子, 篠原 涼平, 佐々木 大介, 佐々木 建吾
    2018年05月, New Food Industry, 60(5) (5), 37 - 43, 日本語
    記事・総説・解説・論説等（学術雑誌）

  • 微粉砕・メタン発酵・高度膜分離による稲わらの利用
    佐々木建吾, 白井智量, 柘植陽太, 佐々木大介, 菊地淳, 近藤昭彦
    2017年03月05日, 日本農芸化学会大会講演要旨集(Web), 2017, ROMBUNNO.4J28a09 (WEB ONLY), 日本語

  • 3P-144 メタン発酵・酸発酵環境における微生物内代謝物の比較(環境工学,廃水処理技術,一般講演)
    佐々木 大介, 佐々木 建吾, 柘植 陽太, 中西 周次, 近藤 昭彦
    日本生物工学会, 2014年, 日本生物工学会大会講演要旨集, 66, 230 - 230, 日本語

  • 1P-219 稲わら前処理液の膜プロセスによるエタノール発酵高効率化(バイオマス,資源,エネルギー工学,一般講演)
    佐々木 建吾, 柘植 陽太, 佐々木 大介, 荻野 千秋, 近藤 昭彦
    日本生物工学会, 2014年, 日本生物工学会大会講演要旨集, 66, 72 - 72, 日本語

  • メタン発酵処理による植物工場の農業廃棄物からのエネルギー回収 : 処理規模とエネルギー収支の評価
    渡邉 淳, 森田 仁彦, 佐々木 大介
    電力中央研究所, 2013年04月, 電力中央研究所報告. 研究報告. 電力中央研究所環境科学研究所 編, (12011) (12011), 巻頭1 - 3,1-19, 日本語

  • 1P-209 微生物群集を対象とした代謝解析による嫌気消化の特徴付け(一般講演(バイオマス,資源,エネルギー工学))
    佐々木 大介, 佐々木 建吾, 中西 周次, 橋本 和仁, 近藤 昭彦
    日本生物工学会, 2013年, 日本生物工学会大会講演要旨集, 65, 70 - 70, 日本語

  • Daisuke Sasaki, Masahiko Morita, Kengo Sasaki, Atsushi Watanabe, Naoya Ohmura
    2013年, Bioscience, Biotechnology and Biochemistry, 77(5) (5), 1096 - 1099, 英語
    [査読有り]
    速報，短報，研究ノート等（学術雑誌）

  • 微生物変換における複合微生物系の利用(その3)セルロース分解菌の添加によるセルロース系有機物からのメタン発酵
    森田 仁彦, 佐々木 大介, 渡邉 淳
    電力中央研究所, 2012年05月, 電力中央研究所報告. 研究報告. 電力中央研究所環境科学研究所 編, (11038) (11038), 1 - 10,巻頭1-3, 日本語

  • S19-2 電気培養によるメタン発酵微生物群集の制御(S19 微生物工学,シンポジウムセッション)
    佐々木 建吾, 佐々木 大介, 森田 仁彦, 五十嵐 泰夫
    日本微生物生態学会, 2012年, 日本微生物生態学会講演要旨集, (28) (28), 127 - 127, 英語

  • 4Ga12 電気化学システムにより制御した水素発酵槽内の微生物叢(環境工学,廃水処理技術,一般講演)
    佐々木 建吾, 森田 仁彦, 佐々木 大介, 松本 伯夫, 大村 直也, 石井 正治, 五十嵐 泰夫
    日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 222 - 222, 日本語

  • 2Ha11 タンパク質共生分解における電気化学的制御の影響(バイオマス,資源,エネルギー工学,一般講演)
    佐々木 大介, 佐々木 建吾, 森田 仁彦, 平野 伸一, 松本 伯夫, 大村 直也
    日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 71 - 71, 日本語

  • 2Ha13 セルロース分解菌の添加によるセルロースからのメタン発酵(バイオマス,資源,エネルギー工学,一般講演)
    森田 仁彦, 佐々木 大介, 渡邉 淳
    日本生物工学会, 2012年, 日本生物工学会大会講演要旨集, 64, 71 - 71, 日本語

  • 微生物変換における複合微生物系の利用(その2)セルロース系有機物のメタン発酵における通電の効果
    渡邉 淳, 森田 仁彦, 佐々木 大介
    電力中央研究所, 2011年05月, 電力中央研究所報告. 研究報告. V, (10019) (10019), 1 - 9,巻頭1～3, 日本語

  • 2Ca03 実廃棄物を用いた通電方固定床メタン発酵の性能評価(環境工学・廃水処理技術/環境浄化・修復・保全技術,一般講演)
    佐々木 大介, 佐々木 建吾, 渡邉 淳, 森田 仁彦, 平野 伸一, 松本 伯夫, 植本 弘明, 大村 直也, 五十嵐 泰夫
    日本生物工学会, 2011年, 日本生物工学会大会講演要旨集, 63, 125 - 125, 日本語

  • 2Ca04 電気化学システムを用いた有機性廃棄物からの水素生成(環境工学・廃水処理技術/環境浄化・修復・保全技術,一般講演)
    佐々木 建吾, 佐々木 大介, 森田 仁彦, 松本 伯夫, 平野 伸一, 大村 直也, 五十嵐 泰夫
    日本生物工学会, 2011年, 日本生物工学会大会講演要旨集, 63, 125 - 125, 日本語

  • 2P-2031 微生物付着担体を含む通電型高温メタン発酵の性能評価(5b 環境工学,廃水処理技術,一般演題,環境バイオテクノロジー,伝統の技と先端科学技術の融合)
    佐々木 大介, 佐々木 建吾, 森田 仁彦, 平野 伸一, 松本 伯夫, 大村 直也, 五十嵐 泰夫
    日本生物工学会, 2010年, 日本生物工学会大会講演要旨集, 22, 114 - 114, 日本語

  • 2P-2030 固定床式メタン発酵のアンモニア耐性(5b 環境工学,廃水処理技術,一般演題,環境バイオテクノロジー,伝統の技と先端科学技術の融合)
    佐々木 建吾, 森田 仁彦, 平野 伸一, 佐々木 大介, 渡邉 淳, 松本 伯夫, 植本 弘明, 大村 直也, 五十嵐 泰夫
    日本生物工学会, 2010年, 日本生物工学会大会講演要旨集, 22, 114 - 114, 日本語

  • P-79 鉄、マンガン添加が高温メタン発酵の酢酸分解に及ぼす影響(E.群集構造解析,ポスター発表)
    山田 千早, 上野 嘉之, 佐々木 大介, 佐々木 建吾, 石井 正治, 五十嵐 泰夫
    日本微生物生態学会, 2010年, 日本微生物生態学会講演要旨集, (26) (26), 134 - 134, 英語

  • 2Hp24 担体への通電が固定床式メタン発酵に及ぼす効果(環境工学・廃水処理技術,一般講演)
    佐々木 建吾, 森田 仁彦, 平野 伸一, 松本 伯夫, 植本 弘明, 大村 直也, 佐々木 大介, 五十嵐 泰夫
    日本生物工学会, 2009年, 日本生物工学会大会講演要旨集, 21, 114 - 114, 日本語

  • 11-202 メタン発酵微生物集団の再組織化機構について(群集構造解析,研究発表)
    堀 知行, 春田 伸, 佐々木 大介, 上野 嘉之, 石井 正治, 五十嵐 泰夫
    日本微生物生態学会, 2008年, 日本微生物生態学会講演要旨集, (24) (24), 159 - 159, 日本語

  • 2Hp02 有機性廃棄物の高効率メタン発酵に関わる細菌群集の構造解析(廃水処理技術,一般講演)
    佐々木 大介, 堀 知行, 春田 伸, 上野 嘉之, 石井 正治, 五十嵐 泰夫
    日本生物工学会, 2008年, 日本生物工学会大会講演要旨集, 20, 198 - 198, 日本語

  • 2Hp03 高温メタン発酵槽における酢酸共生酸化細菌の分子生態解析(廃水処理技術,一般講演)
    堀 知行, 佐々木 大介, 春田 伸, 上野 嘉之, 石井 正治, 五十嵐 泰夫
    日本生物工学会, 2008年, 日本生物工学会大会講演要旨集, 20, 199 - 199, 日本語

  • 2Hp01 担体の表面性質が固定床式メタン発酵に及ぼす影響(廃水処理技術,一般講演)
    佐々木 建吾, 森田 仁彦, 平野 伸一, 松本 伯夫, 植本 弘明, 大村 直也, 佐々木 大介, 春田 伸, 五十嵐 泰夫
    日本生物工学会, 2008年, 日本生物工学会大会講演要旨集, 20, 198 - 198, 日本語

  • 2A14-3 固形分を含む有機性廃棄物の高温嫌気消化におけるメタン生成アーキア群の構造とメタン生成経路の解析(環境工学,廃水処理技術,一般講演)
    佐々木 大介, 春田 伸, 上野 嘉之, 石井 正治, 五十嵐 泰夫
    日本生物工学会, 2007年, 日本生物工学会大会講演要旨集, 19, 47 - 47, 日本語

  • 1B16-3 固形分を含む有機性廃棄物を基質とした完全撹拌混合型メタン発酵槽の菌叢解析(廃水処理技術,一般講演)
    佐々木 大介, 春田 伸, 上野 嘉之, 石井 正治, 五十嵐 泰夫
    日本生物工学会, 2005年, 日本生物工学会大会講演要旨集, 17, 80 - 80, 日本語

  • 2G14-1 有機性廃棄物の可溶化および水素発酵過程に関わる細菌群集構造の解析(廃水処理技術,一般講演)
    佐々木 大介, 上野 喜之, 福井 久智, 春田 伸, 石井 正治, 五十嵐 泰夫
    日本生物工学会, 2004年, 日本生物工学会大会講演要旨集, 16, 202 - 202, 日本語

■ 書籍等出版物
  • Genetic engineering of microorganisms for high glutathione production, Glutathione: Biosynthesis, Functions and Biological Implications
    KOBAYASHI Jyumpei, SASAKI Daisuke, HARA Y. Kiyotaka, KONDO Akihiko
    共著, Nova Science Publishers, 2019年02月, 英語
    学術書

■ 講演・口頭発表等
  • 大麦若葉エキスがヒト大腸細菌叢に与える効果についての検討
    佐々木 建吾, 佐々木 大介, 門脇 靖司, 青塚 康幸, 近藤 昭彦
    日本農芸化学会2020年度大会

  • 微生物燃料電池：アノード電極上のバイオマス量および細胞内グルタミン酸濃度と電流生成の相関
    佐々木 大介, 佐々木 建吾, 柘植 陽太, 近藤 昭彦
    第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議
    口頭発表（一般）

  • 解糖系とペントースリン酸経路への代謝フラックスの分岐比が細胞内代謝プロファイルに及ぼす影響
    村井 克輝, 佐々木 大介, 白井 智量, 内倉 寛斗, 佐々木 建吾, 仁宮 一章, 高橋 憲司, 近藤 昭彦, 柘植 陽太
    第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議
    口頭発表（一般）

  • バイオ電気化学的システムによる複合微生物系の制御
    佐々木 建吾, 佐々木 大介, 森田 仁彦, 柘植 陽太, 近藤 昭彦
    第71回日本生物工学会大会, 2019年09月, 日本語, 岡山大学, 国内会議
    口頭発表（一般）

  • 冠動脈疾患患者大腸フローラモデルによる候補投与物の調査
    佐々木 建吾, 吉田 尚史, 佐々木 大介, 大澤 朗, 山下 智也, 近藤 昭彦
    日本農芸化学会2019年度大会, 2019年03月, 日本語, 東京農業大学 世田谷キャンパス, 国内会議
    ポスター発表

  • E2a5ソルガムバガスの酵素糖化効率支配因子の探索
    川口秀夫, 佐々木大介, 福本妙子, 森田健太, 竹中武蔵, 冨田康平, 佐塚隆志, 松本拓也, 西野孝, 近藤昭彦
    日本農芸化学会 関西・中部支部 2019年度合同神戸大会, 2018年09月, 日本語, 国内会議
    口頭発表（一般）

  • 腸内細菌Bacteroides thetaiotaomicronによる食物繊維の資化性に関する研究
    篠原 涼平, 古根 隆広, 生田 直子, 佐々木 大介, 佐々木 建吾, 福田 伊津子, 大澤 朗, 坂本 憲広
    第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議
    ポスター発表

  • 組換え出芽酵母Saccharomyces cerevisiaeによるキシロースからのグルタチオン生産
    小林 淳平, 佐々木 大介, 番場 崇弘, 蓮沼 誠久, 近藤 昭彦
    第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議
    ポスター発表

  • in vitro培養系ヒト腸内細菌叢モデル（KUHIMM） を用いた難消化性食物繊維の影響評価
    佐々木 大介, 佐々木 建吾, 生田 直子, 福田 伊津子, 近藤 昭彦, 大澤 朗
    第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議
    ポスター発表

  • In vitro培養系ヒト腸内細菌叢モデルによる潰瘍性大腸炎患者の代謝プロファイル異常の検出
    佐々木 建吾, 井上 潤, 星 奈美子, 佐々木 大介, 福田 伊津子, 近藤 昭彦, 大澤 朗
    第70回日本生物工学会大会, 2018年09月, 日本語, 関西大学 千里山キャンパス, 国内会議
    ポスター発表

  • In vitro潰瘍性大腸炎患者大腸フローラモデルの構築
    佐々木 建吾, 井上 潤, 星 奈美子, 佐々木 大介, 近藤 昭彦, 大澤 朗
    日本農芸化学会2018年度大会, 2018年03月, 日本語, 日本農芸化学会, 名古屋市, 国内会議
    ポスター発表

  • 異なる pHで運転した微生物燃料電池：アノード電極上の微生物群集に由来する細胞内代謝物の 比較
    佐々木 大介, 佐々木 建吾, 柘植 陽太, 近藤 昭彦
    第69回日本生物工学会大会, 2017年09月, 日本語, 日本生物工学会, 東京都新宿区, 国内会議
    口頭発表（一般）

  • Effects of indigestible dietary fibers on human intestinal microbiota simulated in a single-batch fermentation system
    佐々木 大介, 佐々木 建吾, 生田 直子, 近藤 昭彦, 大澤 朗
    第21回腸内細菌学会, 2017年06月, 英語, 第21回腸内細菌学会, 神戸市, 国内会議
    口頭発表（一般）

  • 変異チオール酸化酵素による酵母の酸化型グルタチオン 生産
    小林淳平, 佐々木 大介, 原 清敬, 蓮沼 誠久, 近藤 昭彦
    日本農芸化学2017年度大会, 2017年03月, 日本語, 日本農芸化学会, 京都市, 国内会議
    口頭発表（一般）

  • 微粉砕・メタン発酵・高度膜分離による稲わらの利用
    佐々木 建吾, 白井智量, 柘植 陽太, 佐々木 大介, 菊地 淳, 近藤 昭彦
    日本農芸化学2017年度大会, 2017年03月, 日本語, 日本農芸化学会, 京都市, 国内会議
    口頭発表（一般）

  • KUHIMM による難消化性食物繊維の腸内細菌叢への影響評価
    佐々木 大介, 佐々木 建吾, 生田 直子, 近藤 昭彦, 大澤 朗
    第２回デザイン生命工学研究会大会, 2017年03月, 日本語, デザイン生命工学研究会, 神戸市, 国内会議
    口頭発表（一般）

  • ヒト腸管モデルによるタウリンの腸内細菌叢内での動態の検証
    佐々木 建吾, 佐々木 大介, 近藤 昭彦, 大澤朗
    第69回日本細菌学会関西支部総会・学術講演会, 2016年11月, 日本語, 日本細菌学会関西支部, 大阪市, 国内会議
    口頭発表（一般）

  • 微生物燃料電池アノード電極上の微生物群集細胞内代謝解析
    佐々木 大介, 佐々木 建吾, 柘植 陽太, 近藤 昭彦
    第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議
    口頭発表（一般）

  • pH非調整型の培養系ヒト腸管モデルの確立
    佐々木 建吾, 佐々木 大介, 近藤 昭彦, 大澤 朗
    第68回日本生物工学会大会, 2016年09月, 日本語, 日本生物工学会, 富山市, 国内会議
    口頭発表（一般）

  • 膜濃縮したソルガム搾汁液からのエタノール発酵
    佐々木 建吾, 柘植 陽太, 佐々木 大介, 寺村 浩, 川口 秀夫, 荻野 千秋, 春日 重光, 佐塚 隆志, 近藤 昭彦
    日本農芸化学会2015年度大会, 2015年03月, 日本語, 日本農芸化学会, 岡山市, 国内会議
    口頭発表（一般）

  • 稲わら前処理液の膜プロセスによるエタノール発酵高効率化
    佐々木 建吾, 柘植 陽太, 佐々木 大介, 荻野 千秋, 近藤 昭彦
    第66回日本生物工学会大会, 2014年09月, 日本語, 日本生物工学会, 札幌市, 国内会議
    ポスター発表

  • メタン発酵・酸発酵環境における微生物内代謝物の比較
    佐々木 大介, 佐々木 建吾, 柘植 陽太, 中西 周次, 近藤 昭彦
    第66回日本生物工学会大会, 2014年09月, 日本語, 日本生物工学会, 札幌市, 国内会議
    ポスター発表

  • Corynebacterium glutamicum増殖時の電気培養下での乳酸生成
    佐々木 建吾, 柘植 陽太, 佐々木 大介, 近藤 昭彦
    日本農芸化学会2014年度大会, 2014年03月, 日本語, 日本農芸化学会, 川崎市, 国内会議
    口頭発表（一般）

  • 微生物群集を対象とした代謝解析による嫌気消化の特徴付け
    佐々木 大介, 佐々木 建吾, 中西 周次, 橋本 和仁, 近藤 昭彦
    第65回日本生物工学会大会, 2013年09月, 日本語, 日本生物工学会, 広島市, 国内会議
    ポスター発表

  • タンパク質共生分解における電気化学的制御の影響
    佐々木 大介, 佐々木 建吾, 森田 仁彦, 平野 伸一, 松本 伯夫, 大村 直也
    日本生物工学会, 2012年10月, 日本語, 神戸国際会議場, 【目的】我々は電気化学システムを用いた有機性廃棄物からのメタン発酵を評価し、報告してきた。これらの結果から、発酵液を還元電位に制御することによるメタン生成の安定化が明らかとなったが、その効果の由来は定かではなかった。そこで高温メタン発酵槽から分離したタンパク質分解菌と水素資化性メタン菌を用いて通電共培養を行い、通電の影響について評価し、その効果の明確化を試みた。 【方法】250mLの通電培養槽に対して、3電極方式によって－0.8V（vs. Ag/AgCl）に通電しながらスターラーで撹拌を行い、カゼインを炭素源とする培地を用いて55℃で嫌気培養を行った。通電の有り無しで共培養を行うと共に、タンパク質分解菌のみの単菌培養を対照区として計4通りの条件で培養を行い、各種パラメーターを経時的に解析した。 【結果】通電ありの共培養において菌体密度、タンパク質分解, 国内会議
    口頭発表（一般）

■ 共同研究・競争的資金等の研究課題
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