Research activity information

• Oct. 2021 神戸大学農学部六篠会, Rikusou Best Paper Award, Hybrid Rubisco with Complete Replacement of Rice Rubisco Small Subunits by Sorghum Counterparts Confers C4 Plant-like High Catalytic Activity

  深山浩


• Mar. 2019 日本作物学会, Japanese Society of Crop Science Research Award, イネの光合成能力の改良に向けた炭素同化関連タンパク質の生理・生化学的研究

  FUKAYAMA HIROSHI

  Japan society


• Oct. 2016 Rikusou Kai, Rikusou Best Paper Award, CO2-responsive CONSTANS, CONSTANS-like, and time of chlorophyll a/b binding protein Expression1 protein is a positive regulator of starch synthesis in vegetative organs of rice.

  FUKAYAMA HIROSHI

  国際的に評価の高い学術雑誌に論文が掲載されたため．

  Publisher

• Submergence-Tolerant Rice Cultivar FR13A Harbors Floating Genes SNORKELs but Lacks Floating Ability

  Daisuke Sasayama, Natsuki Hayashi, Shunsuke Oe, Hiroshi Fukayama, Tomoko Hatanaka, Tetsushi Azuma

  Elsevier BV, Mar. 2025, Rice Science

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Difference in responsiveness of expressions of starch synthesis-related genes to CRCT among rice cultivars

  Fumihiro Miyagawa, Ryutaro Morita, Mai Tohbe, Daisuke Sasayama, Tomoko Hatanaka, Tetsushi Azuma, Hiroshi Fukayama

  Informa UK Limited, Apr. 2024, Plant Production Science, 27(3) (3), 221 - 228

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of knockout of CO₂-responsive CCT protein, a regulator of starch synthesis on physiological characteristics of rice

  Fumihiro Miyagawa, Naoki Shibatani, Ryutaro Morita, Daisuke Sasayama, Tomoko Hatanaka, Tetsushi Azuma, Hiroshi Fukayama

  Informa UK Limited, Jul. 2023, Plant Production Science, 26(3) (3), 273 - 286

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The relationship between beta-amylase and the degradation of starch temporarily stored in rice leaf blades

  Yu Sugimura, Hiroshi Fukayama, Hiroyasu Michiyama, Tatsuya Hirano

  May 2023, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, English

  Scientific journal


• Deepwater response in the African cultivated rice Oryza glaberrima

  Quanshu Luo, Daisuke Sasayama, Misaki Nakazawa, Tomoko Hatanaka, Hiroshi Fukayama, Tetsushi Azuma

  Informa UK Limited, Jan. 2023, Plant Production Science, 26(1) (1), 1 - 11

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Characterization of the Functions of Starch Synthase IIIb Expressed in the Vegetative Organs of Rice (Oryza sativa L.).

  Ryutaro Morita, Naoko Crofts, Satoko Miura, Ken-Ichi Ikeda, Naohiro Aoki, Hiroshi Fukayama, Naoko Fujita

  Rice is the model C3 crop for investigating the starch biosynthesis mechanism in endosperm because of its importance for grain production. However, little is known about starch biosynthesis in the vegetative organs of rice. In this study, we used novel rice mutants inserting the Tos17 into the starch synthase (SS) IIIb gene, mainly expressed in the leaf sheath and blade, and an ss1 mutant to clarify the differences of roles among SS isozymes during starch biosynthesis. Native-PAGE/activity staining for SS, using leaf sheath and blade of ss mutants, revealed that the lowest migrating SS activity bands on the gel were derived from SSIIIb activity, and that of two ss3b mutants were not detected. The apparent amylose content of leaf sheath starch of ss3b mutants increased. Moreover, the chain-length distribution and size-exclusion chromatography analysis using ss mutants showed that SSIIIb and SSI synthesize the B2-B3 chain and A-B1 chain of amylopectin in the leaf sheath and blade, respectively. Interestingly, we also found that starch contents were decreased in the leaf sheath and blade of ss3b mutants, although SSI deficiency did not affect the starch levels. All these results indicated that SSIIIb synthesizes the long chain of amylopectin in the leaf sheath and leaf blade similarly to SSIIIa in the endosperm, while SSI synthesizes the short chain in the vegetative organ as the same in the endosperm. (226 words).

  Oct. 2022, Plant & cell physiology, English, Domestic magazine

  [Refereed]

  Scientific journal


• Adaptive responses to flooding in wild rice species with various genomes other than AA

  Daisuke Sasayama, Mayuko Niikawa, Tomoko Hatanaka, Hiroshi Fukayama, Tetsushi Azuma

  Informa UK Limited, Jul. 2022, Plant Production Science, 25(3) (3), 350 - 358

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Control of growth direction in crown roots in rice germinated seedlings by light

  Kako Matsumoto, Daisuke Sasayama, Tomoko Hatanaka, Hiroshi Fukayama, Tetsushi Azuma

  Jun. 2022, Journal of Crop Research, 67, 51 - 55

  [Refereed]


• Internodal elongation under submergence in Oryza sativa/Oryza glumaepatula introgression lines

  Daisuke Sasayama, Shangmin Yuan, Tomoko Hatanaka, Hiroshi Fukayama, Tetsushi Azuma

  Jun. 2022, Journal of Crop Research, 67, 35 - 40

  [Refereed]


• Different acyl-CoA:diacylglycerol acyltransferases vary widely in function, and a targeted amino acid substitution enhances oil accumulation

  Tomoko Hatanaka, Yoshiki Tomita, Daisuke Matsuoka, Daisuke Sasayama, Hiroshi Fukayama, Tetsushi Azuma, Mohammad Fazel Soltani Gishini, David Hildebrand

  Abstract Triacylglycerols (TAGs) are the major component of plant storage lipids such as oils. Acyl-CoA:diacylglycerol acyltransferase (DGAT) catalyzes the final step of the Kennedy pathway, and is mainly responsible for plant oil accumulation. We previously found that the activity of Vernonia DGAT1 was distinctively higher than that of Arabidopsis and soybean DGAT1 in a yeast microsome assay. In this study, the DGAT1 cDNAs of Arabidopsis, Vernonia, soybean, and castor bean were introduced into Arabidopsis. All Vernonia DGAT1-expressing lines showed a significantly higher oil content (49% mean increase compared with the wild-type) followed by soybean and castor bean. Most Arabidopsis DGAT1-overexpressing lines did not show a significant increase. In addition to these four DGAT1 genes, sunflower, Jatropha, and sesame DGAT1 genes were introduced into a TAG biosynthesis-defective yeast mutant. In the yeast expression culture, DGAT1s from Arabidopsis, castor bean, and soybean only slightly increased the TAG content; however, DGAT1s from Vernonia, sunflower, Jatropha, and sesame increased TAG content >10-fold more than the former three DGAT1s. Three amino acid residues were characteristically common in the latter four DGAT1s. Using soybean DGAT1, these amino acid substitutions were created by site-directed mutagenesis and substantially increased the TAG content.

  Oxford University Press (OUP), Mar. 2022, Journal of Experimental Botany, 73(9) (9), 3030 - 3043, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• CO2-responsive CCT protein interacts with 14-3-3 proteins and controls the expression of starch synthesis-related genes

  Hiroshi Fukayama, Fumihiro Miyagawa, Naoki Shibatani, Aiko Koudou, Daisuke Sasayama, Tomoko Hatanaka, Tetsushi Azuma, Yasuo Yamauchi, Daisuke Matsuoka, Ryutaro Morita

  Aug. 2021, PLANT CELL AND ENVIRONMENT, 44(8) (8), 2480 - 2493, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Growth responses of seedlings under complete submergence in rice cultivars carrying both the submergence-tolerance gene SUB1A-1 and the floating genes SNORKELs

  Shunsuke Oe, Daisuke Sasayama, Quanshu Luo, Hiroshi Fukayama, Tomoko Hatanaka, Tetsushi Azuma

  Jul. 2021, PLANT PRODUCTION SCIENCE, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of introduction of sorghum RbcS with rice RbcS knockdown by RNAi on photosynthetic activity and dry weight in rice

  Kazuma Sakoda, Akito Yamamoto, Chie Ishikawa, Yojiro Taniguchi, Hiroyoshi Matsumura, Hiroshi Fukayama

  Jul. 2021, PLANT PRODUCTION SCIENCE, 24(3) (3), 346 - 353, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Overexpression of both Rubisco and Rubisco activase rescues rice photosynthesis and biomass under heat stress.

  Yuchen Qu, Kazuma Sakoda, Hiroshi Fukayama, Eri Kondo, Yuji Suzuki, Amane Makino, Ichiro Terashima, Wataru Yamori

  Global warming threatens food security by decreasing crop yields through damage to photosynthetic systems, especially Rubisco activation. We examined whether co-overexpression of Rubisco and Rubisco activase improves the photosynthetic and growth performance of rice under high temperatures. We grew three rice lines-the wild-type (WT), a Rubisco activase-overexpressing line (oxRCA) and a Rubisco- and Rubisco activase-co-overexpressing line (oxRCA-RBCS)-and analysed photosynthesis and biomass at 25 and 40°C. Compared with the WT, the Rubisco activase content was 153% higher in oxRCA and 138% higher in oxRCA-RBCS, and the Rubisco content was 27% lower in oxRCA and similar in oxRCA-RBCS. The CO2 assimilation rate (A) of WT was lower at 40°C than at 25°C, attributable to Rubisco deactivation by heat. On the other hand, that of oxRCA and oxRCA-RBCS was maintained at 40°C, resulting in higher A than WT. Notably, the dry weight of oxRCA-RBCS was 26% higher than that of WT at 40°C. These results show that increasing the Rubisco activase content without the reduction of Rubisco content could improve yield and sustainability in rice at high temperature.

  Mar. 2021, Plant, cell & environment, 44(7) (7), 2308 - 2320, English, International magazine

  [Refereed]

  Scientific journal


• イネRafタイプMAP3K遺伝子Os11g45280は分げつを正に調節し収量の増加に関与する

  松岡大介, 新多智明, 高岡翔平, 三十尾修二, 深山浩, 南森隆司

  Mar. 2021, 大手前大学雑誌「食糧・栄養と健康」, 1, 1 - 6

  [Refereed]

  Research institution


• Hybrid Rubisco with Complete Replacement of Rice Rubisco Small Subunits by Sorghum Counterparts Confers C4 Plant-like High Catalytic Activity.

  Hiroyoshi Matsumura, Keita Shiomi, Akito Yamamoto, Yuri Taketani, Noriyuki Kobayashi, Takuya Yoshizawa, Shun-Ichi Tanaka, Hiroki Yoshikawa, Masaki Endo, Hiroshi Fukayama

  Photosynthetic rate at the present atmospheric condition is limited by the CO2-fixing enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) because of its extremely low catalytic rate (kcat) and poor affinity for CO2 (Kc) and specificity for CO2 (Sc/o). Rubisco in C4 plants generally shows higher kcat than that in C3 plants. Rubisco consists of eight large subunits and eight small subunits (RbcS). Previously, the chimeric incorporation of sorghum C4-type RbcS significantly increased the kcat of Rubisco in a C3 plant, rice. In this study, we knocked out rice RbcS multigene family using the CRISPR-Cas9 technology and completely replaced rice RbcS with sorghum RbcS in rice Rubisco. Obtained hybrid Rubisco showed almost C4 plant-like catalytic properties, i.e., higher kcat, higher Kc, and lower Sc/o. Transgenic lines expressing the hybrid Rubisco accumulated reduced levels of Rubisco, whereas they showed slightly but significantly higher photosynthetic capacity and similar biomass production under high CO2 condition compared with wild-type rice. High-resolution crystal structural analysis of the wild-type Rubisco and hybrid Rubisco revealed the structural differences around the central pore of Rubisco and the βC-βD hairpin in RbcS. We propose that such differences, particularly in the βC-βD hairpin, may impact the flexibility of Rubisco catalytic site and change its catalytic properties.

  Nov. 2020, Molecular plant, 13(11) (11), 1570 - 1581, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Responses to Complete Submergence in African Rice (Oryza glaberrima)

  Quanshu Luo, Misaki Nakazawa, Daisuke Sasayama, Hiroshi Fukayama, Tomoko Hatanaka, Tetsushi Azuma

  Jun. 2020, Journal of Crop Research, 65, 37 - 41

  [Refereed]


• CO2 Responsive CCT Protein Stimulates the Ectopic Expression of Particular Starch Biosynthesis-Related Enzymes, which Markedly Change the Structure of Starch in the Leaf Sheaths of Rice.

  森田 隆太郎, クロフツ 尚子, 柴谷 直樹, 三浦 聡子, 保坂 優子, 追留 那緒子, IKEDA KENICHI, 藤田 直子, FUKAYAMA HIROSHI

  CO2-responsive CCT protein (CRCT) is suggested to be a positive regulator of starch biosynthesis in the leaf sheaths of rice, regulating the expression levels of starch biosynthesis-related genes. In this study, the effects of CRCT expression levels on the expression of starch biosynthesis-related enzymes and the quality of starch were studied. Using native-PAGE/activity staining and immunoblotting, we found that the protein levels of starch synthase I, branching enzyme I, branching enzyme IIa, isoamylase 1 and phosphorylase 1 were largely correlated with the CRCT expression levels in the leaf sheaths of CRCT transgenic lines. In contrast, the CRCT expression levels largely did not affect the expression levels and/or activities of starch biosynthesis-related enzymes in the leaf blades and endosperm tissues. The analysis of the chain-length distribution of starch in the leaf sheaths showed that short chains with a degree of polymerization from 5 to 14 were increased in the overexpression lines but decreased in the knockdown lines. The amylose content of starch in the leaf sheath was greatly increased in the overexpression lines. In contrast, the molecular weight of the amylopectin of starch in the leaf sheath of overexpression lines did not change compared with those of the non-transgenic rice. These results suggest that CRCT can control the quality and the quantity of starch in the leaf sheath by regulating the expression of particular starch biosynthesis-related enzymes.

  Last, May 2019, Plant and Cell Physiology, 60(5) (5), 961 - 972, English, Domestic magazine

  [Refereed]

  Scientific journal


• Short-term effects of high CO2 accelerate photosynthetic induction in Populus koreana x trichocarpa with always-open stomata regardless of phenotypic changes in high CO2 growth conditions

  Hajime Tomimatsu, Tsuyoshi Sakata, Hiroshi Fukayama, Yanhong Tang

  Mar. 2019, TREE PHYSIOLOGY, 39(3) (3), 474 - 483, English

  [Refereed]

  Scientific journal


• Rubisco small subunits of C4 plants, Napier grass and guinea grass confer C4-like catalytic properties on Rubisco in rice.

  FUKAYAMA HIROSHI, Takashi Kobara, Keita Shiomi, Ryutaro Morita, SASAYAMA DAISUKE, HATANAKA TOMOKO, AZUMA TETSUSHI

  2019, Plant Production Science, 22(2) (2), 296 - 300, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Responses of the chloroplast glyoxalase system to high CO2 concentrations.

  Ginga SHIMAKAWA, Kentaro IFUKU, Yuji SUZUKI, Amane MAKINO, Kimitsune ISHIZAKI, Hiroshi FUKAYAMA, Ryutaro MORITA, Katsuhiko SAKAMOTO, Akiko NISHI, Chikahiro MIYAKE

  Dec. 2018, Bioscience, Biotechnology and Biochemistry, 82(12) (12), 2072 - 2083, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Internodal elongation under submergence in the Amazonian wild rice species Oryza glumaepatula: the growth response is induced by hypoxia but not by ethylene

  Daisuke Sasayama, Takuma Okishio, Tatsuya Hirano, Hiroshi Fukayama, Tomoko Hatanaka, Masahiro Akimoto, Tetsushi Azuma

  Springer Science and Business Media LLC, Feb. 2018, Plant Growth Regulation, 85(1) (1), 123 - 132

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Expression level of Rubisco activase negatively correlates with Rubisco content in transgenic rice.

  FUKAYAMA HIROSHI, Akina Mizumoto, Chiaki Ueguchi, Jun Katsunuma, Ryutaro Morita, Daisuke Sasayama, Tomoko Hatanaka, Tetsushi Azuma

  Springer Science and Business Media LLC, 2018, Photosynthesis Research, 137(3) (3), 465 - 474, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Activation State of Rubisco Decreases with the Nitrogen accumulation during the Reproductive Stage in Soybean [Glycine max (L.) Merr.]

  Kazuma Sakoda, Seita Suzuki, FUKAYAMA HIROSHI, Yu Tanaka, Tatsuhiko Shiraiwa

  2018, Photosynthetica, English

  [Refereed]

  Scientific journal


• Overexpression of CO2 responsive CCT protein, a key regulator of starch synthesis strikingly increases the glucose yield from rice straw for bioethanol production

  Ryutaro Morita, Hiroshi Teramura, Chiaki Ogino, Akihiko Kondo, Hiroshi Fukayama

  日本作物学会, Oct. 2017, Plant Production Science, 20(4) (4), 441 - 447, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Promotion of first internode elongation in perennial paddy weeds Sagittaria trifolia and Sagittaria pygmaea tubers by oxygen depletion and carbon dioxide

  Daisuke Sasayama, Kenta Ikemachi, Hisateru Sakai, Hiroshi Fukayama, Kazuyuki Itoh, Tetsushi Azuma

  Dec. 2016, WEED BIOLOGY AND MANAGEMENT, 16(4) (4), 147 - 156, English

  [Refereed]

  Scientific journal


• Identification and expression analysis of non-photosynthetic Rubisco small subunit, OsRbcS1-like genes in plants

  Koichi Morita, Tomoko Hatanaka, Shuji Misoo, Hiroshi Fukayama

  Elsevier, Dec. 2016, Plant Gene, 8, 26 - 31, English

  [Refereed]

  Scientific journal


• Starch Content in Leaf Sheath Controlled by CO2-Responsive CCT Protein is a Potential Determinant of Photosynthetic Capacity in Rice

  Ryutaro Morita, Kanako Inoue, Ken-ichi Ikeda, Tomoko Hatanaka, Shuji Misoo, Hiroshi Fukayama

  Nov. 2016, PLANT AND CELL PHYSIOLOGY, 57(11) (11), 2334 - 2341, English

  [Refereed]

  Scientific journal


• Post-illumination transient O-2-uptake is driven by photorespiration in tobacco leaves

  Takehiro Sejima, Hitomi Hanawa, Ginga Shimakawa, Daisuke Takagi, Yuji Suzuki, Hiroshi Fukayama, Amane Makino, Chikahiro Miyake

  Feb. 2016, PHYSIOLOGIA PLANTARUM, 156(2) (2), 227 - 238, English

  [Refereed]

  Scientific journal


• A Study on the Effect of Maltose in Improving Somatic Embryo Maturation from Long-term Suspension Culture of Soybean

  HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI, KIHARA YUKI, SAKAI TSUNEMITSU, SATA SATOMI

  For micropropagation of soybean, somatic embryo induction from immature cotyledons followed by proliferation, maturation, and conversion culture method is widely used in the world. This protocol is well established with the model cultivar, Jack, however it is also known that the optimal conditions are highly depending on the genotypes. The Japanese elite black soybean, Tanbag

  The Society of Crop Science and Breeding in Kinki, Japan, Nov. 2015, Journal of Crop Research, 60, 47 - 53, Japanese

  [Refereed]

  Scientific journal


• Characterization and expression analyses of two plastidic enolase genes in rice

  Hiroshi Fukayama, Chisato Masumoto, Yojiro Taniguchi, Akiko Baba-Kasai, Yuuki Katoh, Hiroshi Ohkawa, Mitsue Miyao

  Mar. 2015, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 79(3) (3), 402 - 409, English

  [Refereed]

  Scientific journal


• CO2-responsive CONSTANS, CONSTANS-like, and time of chlorophyll ab binding protein Expression1 protein is a positive regulator of starch synthesis in vegetative organs of rice.

  Ryutaro Morita, Miho Sugino, Tomoko Hatanaka, Shuji Misoo, Hiroshi Fukayama

  Feb. 2015, Plant Physiology, 167(4) (4), 1321 - 1331, English

  [Refereed]

  Scientific journal


• Small subunit of a cold-resistant plant, timothy, does not significantly alter the catalytic properties of Rubisco in transgenic rice

  Hiroshi Fukayama, Atsushi Koga, Tomoko Hatanaka, Shuji Misoo

  Kluwer Academic Publishers, 2015, Photosynthesis Research, 124(1) (1), 57 - 65, English

  [Refereed]

  Scientific journal


• Repetitive Short-Pulse Light Mainly Inactivates Photosystem I in Sunflower Leaves

  Takehiro Sejima, Daisuke Takagi, Hiroshi Fukayama, Amane Makino, Chikahiro Miyake

  Jun. 2014, PLANT AND CELL PHYSIOLOGY, 55(6) (6), 1184 - 1193, English

  [Refereed]

  Scientific journal


• A Dominant Mutation in the Light-Oxygen and Voltage2 Domain Vicinity Impairs Phototropin1 Signaling in Tomato1[C][W][OPEN]

  Sulabha Sharma, Eros Kharshiing, Ankanagari Srinivas, Kazunori Zikihara, Satoru Tokutomi, Akira Nagatani, Hiroshi Fukayama, Reddaiah Bodanapu, Rajendra K. Behera, Yellamaraju Sreelakshmi, Rameshwar Sharma

  Apr. 2014, PLANT PHYSIOLOGY, 164(4) (4), 2030 - 2044, English

  [Refereed]

  Scientific journal


• Nocturnal phosphorylation of phosphoenolpyruvate carboxylase in the leaves of hygrophytic C3 monocots.

  FUKAYAMA HIROSHI, Naoko Fujiwara, Tomoko Hatanaka, Shuji Misoo, Mitsue Miyao

  Phosphoenolpyruvate carboxylase (PEPC) undergoes activity regulation through reversible phosphorylation. The day/night phosphorylation of leaf PEPC in 27 C3 plant species was analyzed by immunoblotting. PEPC was phosphorylated in the daytime in 12 species, whereas it was phosphorylated at night in three species, rice, Monochoria vaginalis and Sagittaria trifolia, all of which a

  Feb. 2014, Bioscience, Biotechnology, and Biochemistry, English

  [Refereed]

  Scientific journal


• Soil and Water Warming Accelerates Phenology and Down-Regulation of Leaf Photosynthesis of Rice Plants Grown Under Free-Air CO2 Enrichment (FACE)

  Adachi, Minaco, Hasegawa, Toshihiro, Fukayama, Hiroshi, Tokida, Takeshi, Sakai, Hidemitsu, Matsunami, Toshinori, Nakamura, Hirofumi, Sameshima, Ryoji, Okada, Masumi

  2014, Plant and Cell Physiology, 55(2) (2), 370 - 380

  [Refereed]

  Scientific journal


• Unusual Small Subunit That Is Not Expressed in Photosynthetic Cells Alters the Catalytic Properties of Rubisco in Rice

  Koichi Morita, Tomoko Hatanaka, Shuji Misoo, Hiroshi Fukayama

  Jan. 2014, PLANT PHYSIOLOGY, 164(1) (1), 69 - 79, English

  [Refereed]

  Scientific journal


• Rubisco activase is a key regulator of non-steady-state photosynthesis at any leaf temperature and, to a lesser extent, of steady-state photosynthesis at high temperature

  Wataru Yamori, Chisato Masumoto, Hiroshi Fukayama, Amane Makino

  Sep. 2012, PLANT JOURNAL, 71(6) (6), 871 - 880, English

  [Refereed]

  Scientific journal


• Photosynthetic Characteristics of Antisense Transgenic Rice Expressing Reduced Levels of Rubisco Activase

  Chisato Masumoto, Hiroshi Fukayama, Tomoko Hatanaka, Naotsugu Uchida

  Jul. 2012, PLANT PRODUCTION SCIENCE, 15(3) (3), 174 - 182, English

  [Refereed]

  Scientific journal


• Overexpression of Rubisco Activase Decreases the Photosynthetic CO2 Assimilation Rate by Reducing Rubisco Content in Rice Leaves

  Hiroshi Fukayama, Chiaki Ueguchi, Kaoru Nishikawa, Nobuaki Katoh, Chie Ishikawa, Chisato Masumoto, Tomoko Hatanaka, Shuji Misoo

  Jun. 2012, PLANT AND CELL PHYSIOLOGY, 53(6) (6), 976 - 986, English

  [Refereed]

  Scientific journal


• Identification of Two Plastid-Targeted beta-Amylases in Rice

  Tatsuya Hirano, Yusuke Takahashi, Hiroshi Fukayama, Hiroyasu Michiyama

  Oct. 2011, PLANT PRODUCTION SCIENCE, 14(4) (4), 318 - 324, English

  [Refereed]

  Scientific journal


• Gene expression profiling of rice grown in free air CO2 enrichment (FACE) and elevated soil temperature

  Hiroshi Fukayama, Miho Sugino, Takuya Fukuda, Chisato Masumoto, Yojiro Taniguchi, Masumi Okada, Ryoji Sameshima, Tomoko Hatanaka, Shuji Misoo, Toshihiro Hasegawa, Mitsue Miyao

  Feb. 2011, FIELD CROPS RESEARCH, 121(1) (1), 195 - 199, English

  [Refereed]

  Scientific journal


• Cyclic electron flow around PSI functions in the photoinhibited rice leaves

  Satoshi Kubo, Takehiro Masumura, Yuhi Saito, Hiroshi Fukayama, Yuji Suzuki, Toshio Sugimoto, Amane Makino, Katsumi Amako, Chikahiro Miyake

  Feb. 2011, SOIL SCIENCE AND PLANT NUTRITION, 57(1) (1), 105 - 113, English

  [Refereed]

  Scientific journal


• Functional Incorporation of Sorghum Small Subunit Increases the Catalytic Turnover Rate of Rubisco in Transgenic Rice

  Ishikawa C, Hatanaka T, Misoo S, Miyake C, Fukayama H

  2011, Plant Physiology, 156(3) (3), 1603 - 1611, English

  [Refereed]

  Scientific journal


• SDS-dependent proteases induced by ABA and its relation to Rubisco and Rubisco activase contents in rice leaves

  Hiroshi Fukayama, Rie Abe, Naotsugu Uchida

  Oct. 2010, PLANT PHYSIOLOGY AND BIOCHEMISTRY, 48(10-11) (10-11), 808 - 812, English

  [Refereed]

  Scientific journal


• Phosphoenolpyruvate carboxylase intrinsically located in the chloroplast of rice plays a crucial role in ammonium assimilation

  MASUMOTO C, MIYAZAWA SI, OHKAWA H, FUKUDA T, TANIGUCHI Y, MURAYAMA S, KUSANO M, SAITO K, FUKAYAMA H, MIYAO M

  2010, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107(11) (11), 5226 - 5231

  [Refereed]


• Screening of High k(cat) Rubisco among Poaceae for Improvement of Photosynthetic CO2 Assimilation in Rice

  Chie Ishikawa, Tomoko Hatanaka, Shuji Misoo, Hiroshi Fukayama

  Jul. 2009, PLANT PRODUCTION SCIENCE, 12(3) (3), 345 - 350, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Rice plant response to long term CO3 enrichment: Gene expression profiling

  Hiroshi Fukayama, Takuya Fukuda, Chisato Masumoto, Yojiro Taniguchi, Hidemitsu Sakai, Weiguo Cheng, Toshihiro Hasegawa, Mitsue Miyao

  Elsevier, 2009, PLANT SCIENCE, 177(3) (3), 203 - 210, English

  [Refereed]

  Link to Kobe Univ. Repository (Kernel)


• Overproduction of C4 photosynthetic enzymes in transgenic rice plants: an approach to introduce the C4-like photosynthetic pathway into rice

  Taniguchi Y, Ohkawa H, Masumoto C, Fukuda T, Tamai T, Lee K, Sudoh S, Tsuchida H, Sasaki H, Fukayama H, Miyao M

  2008, Journal of Experimental Botany, 59(7) (7), 1799 - 1809

  [Refereed]


• Identification of C4 responsive genes in the facultative C4 plant Hydrilla verticillata.

  Srinath K. Rao, Hiroshi Fukayama, Julia B. Reiskind, Mitsue Miyao, George Bowes

  Lead, Jul. 2006, Photosynthesis Research, 88, 173 - 183, English

  [Refereed]

  Scientific journal


• Characterization and functional analysis of phosphoenolpyruvate carboxylase kinase genes in rice.

  Hiroshi Fukayama, Tesshu Tamai, Yojiro Taniguchi, Stuart Sullivan, Mitsue Miyao, Hugh G. Nimmo

  Apr. 2006, The Plant Journal, 47, 258 - 268, English

  [Refereed]

  Scientific journal


• Activity regulation and physiological impacts of maize C(4)-specific phosphoenolpyruvate carboxylase overproduced in transgenic rice plants

  H Fukayama, MD Hatch, T Tamai, H Tsuchida, S Sudoh, RT Furbank, M Miyao

  Lead, 2003, PHOTOSYNTHESIS RESEARCH, 77(2-3) (2-3), 227 - 239, English

  [Refereed]

  Scientific journal


• Significant accumulation of C-4-specific pyruvate,orthophosphate dikinase in a C-3 plant, rice

  H Fukayama, H Tsuchida, S Agarie, M Nomura, H Onodera, K Ono, BH Lee, S Hirose, S Toki, MSB Ku, A Makino, M Matsuoka, M Miyao

  Lead, Nov. 2001, PLANT PHYSIOLOGY, 127(3) (3), 1136 - 1146, English

  [Refereed]

  Scientific journal


• High level expression of C-4-specific NADP-malic enzyme in leaves and impairment of photoautotrophic growth in a C-3 plant, rice

  H Tsuchida, T Tamai, H Fukayama, S Agarie, M Nomura, H Onodera, K Ono, Y Nishizawa, BH Lee, S Hirose, S Toki, MSB Ku, M Matsuoka, M Miyao

  Feb. 2001, PLANT AND CELL PHYSIOLOGY, 42(2) (2), 138 - 145, English

  [Refereed]

  Scientific journal


• High-level expression of maize phosphoenolpyruvate carboxylase in transgenic rice plants

  Ku, M.S.B, Agarie, S, Nomura, M, Fukayama, H, Hirose, S, Toki, S, Miyao-Tokutomi, M, Matsuoka, M

  Jan. 1999, 17, 76 - 80

  [Refereed]


• Light dependent activation of CO₂ assimilation and the ratio of Rubisco activase to Rubisco during leaf aging of rice (Oryza sativa )

  Hiroshi Fukayama, Naotsugu Uchida, Tetsushi Azuma, Takeshi Yasuda

  Lead, Wiley, Dec. 1998, Physiologia Plantarum, 104(4) (4), 541 - 548

  [Refereed]

  Scientific journal


• Salinity tolerance in Triticum aestivum-Lophopyrum elongatum amphiploid and 5E disomic addition line evaluated by NaCl effects on photosynthesis and respiration

  Kazue Kasai, Hiroshi Fukayama, Naotsugu Uchida, Naoki Mori, Takeshi Yasuda, Yoshikiyo Oji, Chiharu Nakamura

  Springer Science and Business Media LLC, Sep. 1998, Cereal Research Communications, 26(3) (3), 281 - 287

  [Refereed]

  Scientific journal


• Relationships between Photosynthetic Activity and the Amounts of Rubisco Activase and Rubisco in Rice Leaves from Emergence through Senescence.

  Hiroshi FUKAYAMA, Naotsugu UCHIDA, Tetsushi AZUMA, Takeshi YASUDA

  Lead, Crop Science Society of Japan, 1996, Japanese Journal of Crop Science, 65(2) (2), 296 - 302

  [Refereed]

  Scientific journal

• Responsiveness of Expression of Starch Synthesis-Related Genes to CRCT in Vegetative Organs of Rice

  宮川文宏, 森田隆太郎, 笹山大輔, 畠中知子, 東哲司, 深山浩

  2023, 日本作物学会講演会要旨集, 255th


• Responses to phytohormones in Potato StCRCT-overexpressing line

  松下大輝, 永山大貴, 志村華子, 森田隆太郎, 深山浩, 藤野介延

  2022, 日本作物学会講演会要旨集, 254th


• Improvement of photosynthetic capacity and crop productivity by the enhancement of Rubisco catalytic activity in rice

  Hiroshi Fukayama, Hiroyoshi Matsumura

  Lead, Aug. 2021, News letter, 31(2) (2), 110 - 119

  [Refereed]


• Functional Analysis of Granule-bound Starch Synthase (GBSS) II, Which Expressed in Vegetative Organs of Rice (Oryza Sativa. L)

  森田隆太郎, 高橋陽希, 三浦聡子, CROFTS Naoko, CROFTS Naoko, 深山浩, 青木直大, 藤田直子

  2021, 日本作物学会講演会要旨集, 251st


• イネの栄養器官で発現する澱粉粒結合型スターチシンターゼ(GBSS)IIの機能解析

  高橋陽希, 森田隆太郎, 三浦聡子, クロフツ尚子, 青木直大, 深山浩, 藤田直子

  2021, 応用糖質科学, 11(2) (2)


• イネ栄養器官特異的に発現するスターチシンターゼ(SS)IIIbの機能解析

  森田隆太郎, 三浦聡子, クロフツ尚子, 青木直大, 深山浩, 藤田直子

  2020, 応用糖質科学, 10(1) (1)


• イネの栄養器官で発現する澱粉粒結合型スターチシンターゼ(GBSS)IIの機能解析

  高橋陽希, 森田隆太郎, 三浦聡子, クロフツ尚子, 青木直大, 深山浩, 藤田直子

  2020, 応用糖質科学, 10(4) (4)


• イネ栄養器官で特異的に発現するスターチシンターゼ(SS)IIIbの機能解析

  森田隆太郎, 三浦聡子, クロフツ尚子, 青木直大, 深山浩, 藤田直子

  2019, 応用糖質科学, 9(3) (3)


• イネの根における糖・デンプン代謝関連遺伝子の発現解析

  植田夏実, 小島直子, 深山浩, 森田隆太郎, 廣瀬竜郎, 山岸順子, 青木直大

  2019, 日本作物学会講演会要旨集, 248th


• 澱粉生合成酵素SSI,IIIbの変異体を用いたイネ栄養器官における澱粉生合成機構の解析

  森田隆太郎, 森田隆太郎, 三浦聡子, クロフツ尚子, クロフツ尚子, 青木直大, 池田健一, 深山浩, 藤田直子

  2019, 日本作物学会講演会要旨集, 247th


• バレイショにおけるデンプン合成制御因子オーソログStCRCTの過剰発現体の形態観察

  永山大貴, 林龍之介, 横山音, 志村華子, 森田隆太郎, 深山浩, 藤野介延

  2019, 日本作物学会講演会要旨集, 248th


• バレイショにおけるイネCRCT遺伝子のオーソログの解析

  林龍之介, 横山音, 津釜大侑, 森田隆太郎, 森田隆太郎, 深山浩, 藤野介延

  2018, 日本作物学会講演会要旨集, 245th


• イネ栄養器官特異的に発現する澱粉生合成酵素IIIbの機能解析

  森田隆太郎, 森田隆太郎, 三浦聡子, CROFT Naoko, CROFT Naoko, 青木直大, 深山浩, 藤田直子

  2018, 日本作物学会講演会要旨集, 246th


• CO₂応答性CCTタンパク質CRCTはイネ栄養器官のデンプン合成制御に働く

  森田隆太郎, 森田隆太郎, 深山浩

  2017, 日本作物学会講演会要旨集, 243rd


• デンプン合成制御因子CRCTが稲わらを用いたバイオエタノール生産に及ぼす効果

  森田隆太郎, 森田隆太郎, 寺村浩, 荻野千秋, 近藤昭彦, 深山浩

  2017, 日本作物学会講演会要旨集, 243rd


• Changes in the Content of OsRca2 During Leaf Development and Senescence

  Mizumoto Akina, Hatanaka Tomoko, Misoo Shuji, Fukayama Hiroshi

  CROP SCIENCE SOCIETY OF JAPAN, 2016, Abstracts of Meeting of the CSSJ, 241, 193 - 193, Japanese


• C3 to C4 : Is C4 Rubisco alone useful?

  Hiroshi Fukayama

  Lead, 2016, Plant Science Frontier, 7, 28 - 34


• Functional Analysis of Rubisco Activase-like Protein OsRca2 in Rice

  Fukayama Hiroshi, Kobayashi Akiko, Mizumoto Akina, Hatanaka Tomoko, Misoo Shuji

  CROP SCIENCE SOCIETY OF JAPAN, 2015, Abstracts of Meeting of the CSSJ, 240, 39 - 39, Japanese


• デンプン合成の制御因子CRCTの発現量がイネの生育と光合成に与える影響

  森田隆太郎, 畠中知子, 三十尾修司, 深山浩

  2014, 日本作物学会講演会要旨集, 238th


• 高CO₂条件下で発現促進されるCCTタンパク質CRCTはイネの栄養器官におけるデンプン合成の調節因子である

  森田隆太郎, 杉野充保, 畠中知子, 三十尾修司, 深山浩

  2014, 日本作物学会講演会要旨・資料集, 237th


• Evaluation of Salt Tolerance during Seedling Stage in Doubled Haploid Lines Obtained from F_1 of Salt Tolerant Rice cv. Pokkali

  UMEZAKI Yurika, NISHIMURA Koichiro, FUKAYAMA Hiroshi, HATANAKA Tomoko, MISOO Shuji

  09 Sep. 2013, 日本作物學會紀事, 82, 84 - 85, Japanese


• Functional Analysis of OsRbcS1, a Novel Rubisco Small Subunit that is Not Expressed in Leaf Blade in Rice

  MORITA Koichi, HATANAKA Tomoko, MISOO Shuji, FUKAYAMA Hiroshi

  28 Mar. 2013, 日本作物學會紀事, 82, 182 - 183, Japanese


• Characterization of Double Transgenic Rice that Overexpress the Small Subunit of Sorghum High kcat Rubisco with Reduced Rubisco Content by RNAi Knockdown

  FUKAYAMA Hiroshi, ISHIKAWA Chie, HATANAKA Tomoko, MISOO Shuji

  28 Mar. 2013, 日本作物學會紀事, 82, 180 - 181, Japanese


• Rubisco Activase Down-regulates the Accumulation of Rubisco by Inhibiting a Process Required for Rubisco Biosynthesis

  UEGUCHI Chiaki, HATANAKA Tomoko, MISOO Shuji, FUKAYAMA Hiroshi

  28 Mar. 2013, 日本作物學會紀事, 82, 184 - 185, Japanese


• A Novel CCT Domain Containing Protein CRCT Up-regulated under Elevated CO_2 Controls the Expression of Primary Metabolism Genes and the Tiller Angle in Rice

  MORITA Ryutaro, SUGINO Miho, HATANAKA Tomoko, MISOO Shuji, FUKAYAMA Hiroshi

  28 Mar. 2013, 日本作物學會紀事, 82, 186 - 187, Japanese


• 植物の高CO2応答における遺伝子発現変化

  FUKAYAMA HIROSHI

  Lead, 日本農芸化学会 ; 1962-, 2013, 化学と生物, 51(10) (10), 710 - 716, Japanese

  [Refereed]

  Introduction scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 高CO2環境に適したRubiscoの導入によるイネの光合成能力の改良

  FUKAYAMA HIROSHI

  Lead, 日本光合成学会, 2013, 光合成研究, 23(1) (1), 24 - 32, Japanese

  [Refereed]

  Introduction scientific journal


• 光合成誘導反応

  唐 艶鴻, 冨松 元, FUKAYAMA HIROSHI

  2013, 化学と生物, 52, 106 - 112, Japanese

  [Refereed][Invited]

  Introduction scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Functional Analysis of Receptor-like Kinase OsTPK1 that is Upregulated in Response to Elevated CO_2 in Rice

  HIROSE Yui, HATANAKA Tomoko, MISOO Shuji, FUKAYAMA Hiroshi

  29 Mar. 2012, 日本作物學會紀事, 81, 146 - 147, Japanese


• Comparison of Salt Tolerance between Parents and Doubled Haploid Lines in Indica Rice during Seedling Stage

  NISHIMURA Koichiro, FUKAYAMA Hiroshi, HATANAKA Tomoko, MISOO Shuji

  29 Mar. 2012, 日本作物學會紀事, 81, 318 - 319, Japanese


• イネにおいて高CO₂条件下で発現促進される新規CCTタンパク質OsCCT1の機能解析

  深山浩, 森田隆太郎, 杉野充保, 畠中知子, 三十尾修司

  2012, 日本植物生理学会年会要旨集, 53rd


• Lessons from engineering a single-cell C-4 photosynthetic pathway into rice

  Mitsue Miyao, Chisato Masumoto, Shin-Ichi Miyazawa, Hiroshi Fukayama

  Last, May 2011, JOURNAL OF EXPERIMENTAL BOTANY, 62(9) (9), 3021 - 3029, English

  [Refereed]

  Book review


• Molecular and Functional Characterization of OsPGMd2 Encoding Chloroplast-Localized Phosphoglycerate Mutase in Rice

  KATOH Yuuki, FUKAYAMA Hiroshi, OHKAWA Hiroshi, TANIGUCHI Yojiro, MASUMOTO Chisato, MIYAO Mitsue, HATANAKA Tomoko, MISOO Shuji

  30 Mar. 2011, 日本作物學會紀事, 80, 144 - 145, Japanese


• Novel CCT Domain Containing Protein OsCCT1 is Involved in Metabolic and Morphological Responses to Long Term CO_2 Enrichment in Rice

  SUGINO Miho, FUKAYAMA Hiroshi, SAKAI Hidemitsu, HATANAKA Tomoko, MISOO Shuji

  30 Mar. 2011, 日本作物學會紀事, 80, 142 - 143, Japanese


• Molecular and Functional Characterization of Genes Encoding Plastid-Localized Enolase in Rice

  FUKAYAMA Hiroshi, KATOH Yuuki, SHINTANI Shyota, TANIGUCHI Yojiro, MASUMOTO Chisato, HATANAKA Tomoko, MISOO Shuji, MIYAO Mitsue, OHKAWA Hiroshi

  30 Mar. 2010, 日本作物學會紀事, 79, 102 - 103, Japanese


• イネでの、葉緑体光合成電子伝達系の窒素(N)栄養応答(9.植物の多量栄養素,2009年度京都大会)

  真野 陽人, 渡辺 竜馬, 桑原 亮, 増村 威宏, 斉藤 雄飛, 尼子 克己, 深山 浩, 杉本 敏男, 三宅 親弘

  一般社団法人日本土壌肥料学会, 15 Sep. 2009, 日本土壌肥料学会講演要旨集, (55) (55), 72 - 72, Japanese


• Effects of Overexpression of Barley Rubisco Activase on Photosynthetic Characteristics in Rice

  FUKAYAMA Hiroshi, NISHIKAWA Kaoru, HATANAKA Tomoko, MISOO Shuji

  27 Mar. 2009, 日本作物學會紀事, 78, 214 - 215, Japanese


• Effects of season-long Free Air CO_2 Enrichment (FACE) and soil warming on leaf photosynthesis of rice

  ADACHI Minaco, FUKAYAMA Hiroshi, TOKIDA Takeshi, CHENG Weiguo, NAKAMURA Hirofumi, OKAWARA Yoshinobu, SAMESHIMA Ryoji, OKADA Masumi, HASEGAWA Toshihiro

  27 Mar. 2009, 日本作物學會紀事, 78, 230 - 231, Japanese


• P-39 Effects of different kinds of sugar and amino acids on maturation culture of black soybean, 'Hyokeikuro-3'

  Kihara Yuki, Hatanaka Tomoko, Fukayama Hiroshi, Misoo Shuji

  The Crop Science Society of Japan, 24 Sep. 2008, Japanese Journal of Crop Science, 77(2) (2), 292 - 293, Japanese


• P-27 Characterization of transgenic rice plants overproducing four different enzymes related to C_4 photosynthesis

  Masumoto Chisato, Taniguchi Yojiro, Fukuda Takuya, Ohkawa Hiroshi, Sasaki Haruto, Fukayama Hiroshi, Miyao Mitsue

  The Crop Science Society of Japan, 24 Sep. 2008, Japanese Journal of Crop Science, 77(2) (2), 268 - 269, Japanese


• P-40 Oil crop, Jatropha curcas : Proliferation via tissue culture and comparison of seed oil contents

  Kurimoto Atsuko, Hatanaka Tomoko, Fukayama Hiroshi, Misoo Shuji, Sambongi Kazuo

  The Crop Science Society of Japan, 24 Sep. 2008, Japanese Journal of Crop Science, 77(2) (2), 294 - 295, Japanese


• P-24 Screening of High k_ Rubisco among Poaceae for Improvement of Photosynthetic Capacity of Rice by Genetic Recombination

  Fukayama Hiroshi, Ishikawa Chie, Hatanaka Tomoko, Misoo Shuji

  The Crop Science Society of Japan, 24 Sep. 2008, Japanese Journal of Crop Science, 77(2) (2), 262 - 263, Japanese


• Gene Expression Profiling in Rice Grown under Free-Air CO_2 Enrichment (FACE) and Elevated Paddy Field Temperature

  FUKAYAMA Hiroshi, FUKUDA Takuya, MASUMOTO Chisato, TANIGUCHI Yojiro, HASEGAWA Toshihiro, MIYAO Mitsue

  The Crop Science Society of Japan, 27 Mar. 2008, Japanese Journal of Crop Science, 77, 274 - 275, Japanese


• P10-1 高CO_2条件で育成したイネにおける遺伝子発現プロファイル(10.植物の代謝,2007年度東京大会)

  深山 浩, 福田 琢哉, 酒井 英光, 程 為国, 谷口 洋二郎, 宮尾 光恵, 長谷川 利拡

  一般社団法人日本土壌肥料学会, 22 Aug. 2007, 日本土壌肥料学会講演要旨集, (53) (53), 95 - 95, Japanese


• Introduction of C4 photosynthetic pathway into rice

  Yojiro Taniguchi, Hiroshi Ohkawa, Takuya Fukuda, Chisato Masumoto, Hiroshi Fukayama, Mitsue Miyao-Tokutomi

  2007, PLANT AND CELL PHYSIOLOGY, 48, S174 - S174, English

  Summary international conference


• GENES FOR ENZYMES INVOLVED IN CARBON METABOLISM : LESSONS FROM HIGHER PLANT GENOMES

  MIYAO TOKUTOMI Mitsue, OHKAWA Hiroshi, TANIGUCHI Yojiro, FUKAYAMA Hiroshi

  01 Dec. 2006, Journal of plant research, 119, 13 - 13, English


• Introduction of C4 photosynthetic cycle into C3 plants. Feasibility, problems and perspectives

  Tokutomi-Miyao Mitsue, Hiroshi Fukayama

  Last, 2005, 43(10) (10), 642 - 647


• 135 Identification of the Genes Necessary for the Single-Cell C_4 Photosynthesis System in a Submersed Aquatic Macrophyte, Hydrilla verticillata

  Fukayama Hiroshi, K. Rao Srinath, B. Reiskind Julia, Miyao Mitsue, Bowes George

  The Crop Science Society of Japan, 20 Oct. 2004, Japanese Journal of Crop Science, 73(2) (2), 270 - 271, Japanese


• Metabolic consequences of overproduction of phosphoenolpyruvate carboxylase in C3 plants

  Mitsue Miyao, Hiroshi Fukayama

  Last, Academic Press Inc., 15 Jun. 2003, Archives of Biochemistry and Biophysics, 414(2) (2), 197 - 203, English

  [Refereed]

  Book review


• Overexpression of C_4 Photosynthetic Enzymes in Rice Plants

  Fukayama Hiroshi

  The Crop Science Society of Japan, 02 Apr. 2002, Japanese Journal of Crop Science, 71(1) (1), 296 - 297, Japanese


• MOLECULAR ENGINEERING OF C4 PHOTOSYNTHESIS.

  Makoto Matsuoka, Robert T Furbank, Hiroshi Fukayama, Mitsue Miyao

  The majority of terrestrial plants, including many important crops such as rice, wheat, soybean, and potato, are classified as C3 plants that assimilate atmospheric CO2 directly through the C3 photosynthetic pathway. C4 plants such as maize and sugarcane evolved from C3 plants, acquiring the C4 photosynthetic pathway to achieve high photosynthetic performance and high water- and nitrogen-use efficiencies. The recent application of recombinant DNA technology has made considerable progress in the molecular engineering of C4 photosynthesis over the past several years. It has deepened our understanding of the mechanism of C4 photosynthesis and provided valuable information as to the evolution of the C4 photosynthetic genes. It also has enabled us to express enzymes involved in the C4 pathway at high levels and in desired locations in the leaves of C3 plants for engineering of primary carbon metabolism.

  Jun. 2001, Annual review of plant physiology and plant molecular biology, 52, 297 - 314, English, International magazine

  [Refereed]


• CHARACTERIZATION OF TRANSGENIC RICE PLANTS THAT OVERPRODUCE THE MAIZE C_4-SPECIFIC PHOSPHOENOLPYRUVATE CARBOXYLASE : II. EFFECTS ON PHOTOSYNTHESIS AND RESPIRATION :

  FUKAYAMA Hiroshi, TAMAI Tesshu, TSUCHIDA Hiroko, MATSUOKA Makoto, TOKUTOMI Mitsue

  Japanese Society of Plant Physiologists, 2001, Plant and cell physiology, 42, s157, English


• CHARACTERIZATION OF TRANSGENIC RICE PLANTS THAT OVERPRODUCE THE MAIZE C4-SPECIFIC POSPHOENOLPYRUVATE CARBOXYLASE : I. BIOCHEMICAL ANALYSES :

  TAMAI Tesshu, FUKAYAMA Hiroshi, TSUCHIDA Hiroko, IZUI Katsura, TOKUTOMI Mitsue

  Japanese Society of Plant Physiologists, 2001, Plant and cell physiology, 42, s157, English


• Improvement of Photosynthetic System in Rice Leaves

  FUKAYAMA Hiroshi, UCHIDA Naotsugu

  Lead, Kobe University, 25 Feb. 2000, The science reports of Faculty of Agriculture, Kobe University, 24(1) (1), 11 - 21, Japanese


• Effect of High Level Expression of the Maize Pyruvate,Orthophosphate Dikinase on Photosynthesis Rate and Nitrogen Partitioning in Transgenic Rice Plants.

  Fukayama H., Tuchida H., Onodera H., Ono K., Makino A., Matsuoka M., Tokutomi-Miyao M.

  The Crop Science Society of Japan, 01 Oct. 1999, Japanese Journal of Crop Science, 68(2) (2), 80 - 81, Japanese


• High-level expression of maize phosphoenolpyruvate carboxylase in transgenic rice plants

  MSB Ku, S Agarie, M Nomura, H Fukayama, H Tsuchida, K Ono, S Hirose, S Toki, M Miyao, M Matsuoka

  Jan. 1999, NATURE BIOTECHNOLOGY, 17(1) (1), 76 - 80, English

  [Refereed]


• Changes in the Rate of Catalytic Turnover and the Number of Carbamylated Site of Rubisco during Leaf Aging of Rice

  FUKAYAMA Hiroshi, UCHIDA Naotsugu, AZUMA Tetsushi, YASUDA Takeshi

  The Crop Science Society of Japan, 02 Apr. 1998, Japanese Journal of Crop Science, 67, 104 - 105, Japanese


• 68 Photosynthetic Characteristics in Wild Rice Species : The in vivo Activity of Rubisco

  HIRAOKA M., UCHIDA N., FUKAYAMA H., AZUMA T., YASUDA T.

  野生イネO.barthiiとO.meridionalisのin vivoのRubisco比活性は栽培種のそれより有意に高く, Rubiscoの活性比率とRubisco activase含量も高い。

  The Crop Science Society of Japan, 18 Oct. 1997, Japanese Journal of Crop Science, 66(2) (2), 135 - 136, Japanese


• 67 Relationship between Specific Degradation of Rubisco and Increased Proteolytic Activity in a Rice Leaf Piece Induced by ABA

  FUKAYAMA Hiroshi, ARAI Rie, UCHIDA Naotsugu, AZUMA Tetsushi, YASUDA Takeshi

  The Crop Science Society of Japan, 02 Apr. 1997, Japanese Journal of Crop Science, 66(1) (1), 134 - 135, Japanese


• 66 Relaxation Time for Activation of CO_2 Exchange Decreases with Leaf Aging and It is Related to Rubisco activase/Rubisco Ratio

  FUKAYAMA Hiroshi, UCHIDA Naotsugu, AZUMA Tetsushi, YASUDA Takeshi

  The Crop Science Society of Japan, 02 Apr. 1997, Japanese Journal of Crop Science, 66(1) (1), 132 - 133, Japanese


• 88 Photosynthetic characteristics in wild rice species

  Hiraoka Motoaki, Fukayama Hiroshi, Uchida Naotsugu, Azuma Tetsushi, Yasuda Takeshi

  The Crop Science Society of Japan, 02 Oct. 1996, Japanese Journal of Crop Science, 65(2) (2), 175 - 176, Japanese


• Relationships between Photosynthetic Activity and the Amounts of Rubisco Activase and Rubisco in Rice Leaves from Emergence through Senescence

  FUKAYAMA Hiroshi, UCHIDA Naotsugu, AZUMA Tetsushi, YASUDA Takeshi

  Photosynthetic activity determined as O_2 evolution (OER) and the amounts of Rubisco activase, Rubisco, total soluble protein and chlorophyll were investigated in the 10th leaf of rice, Oryza sativa L. cv. Nipponbare, in relation to its aging. The Rubisco activase content increased until the 17th day after leaf emergence, at which time it attained its maximum and accounted for 1.43% of total soluble protein; thereafter, it decreased rapidly. This change was most remarkable compared to the other leaf constituents examined. The Rubisco content had already reached its maximum 3 days after leaf emergence and had begun to decrease earliest among the leaf constituents. The OER depended linearly on the leaf Rubisco content below the value of 3 g m^<-2>, but tended to saturate above this value. On the contrary, the amount of Rubisco activase and OER were linearly correlated during the life span of the leaf. The in vivo Rubisco activity, as the OER per unit Rubisco content, increased exponentially with the increased Rubisco activase/Rubisco ratio. These results show that the amount of Rubisco activase is closely related to the photosynthetic rates in rice leaf from its emergence through senescence, and suggest that in vivo Rubisco activity can be restricted by Rubisco activase content, particularly when the leaf is young and accumulates excess Rubisco.

  The Crop Science Society of Japan, 05 Jun. 1996, Jpn. J. Crop Sci., 65(2) (2), 296 - 302, English


• 42 Effects of Plant growth regulators on photosynthetic activity of senescing rice leaf slices : Rubisco activase, Rubisco and proteinase

  FUKAYAMA Hiroshi, UCHIDA Naotsugu, AZUMA Tetsushi, YASUDA Takeshi

  The Crop Science Society of Japan, 03 Apr. 1996, Japanese Journal of Crop Science, 65(1) (1), 84 - 85, Japanese


• 48 Relation between photosynthetic activity and Rubisco activase content in rice leaves from emergence through senescence

  Fukayama Hiroshi, Uchida Naotsugu, Azuma Tetsushi, Yasuda Takeshi

  イネ葉の加齢に伴う光合成速度の変化はRubisco activase含量との関係が最も密接で, in vivoの活性はRubiscoに対するRubisco activaseの比率が高いほど高い.

  The Crop Science Society of Japan, 03 Apr. 1995, Japanese Journal of Crop Science, 64(1) (1), 96 - 97, Japanese


• Purification of Rubisco activase from rice leaves

  FUKAYAMA H., Uchida Naotsugu, Azuma Tetsushi, Yasuda Takeshi, Yamaguchi Tadashi

  ゲルろ過とイオン交換クロマトグラフィーにより, イネ葉より Rubisco activase を精製することができた. 12.5% SDS-PAGE の結果それは約40 kDa のペプチドであることがわかった.

  The Crop Science Society of Japan, 1994, Jpn. J. Crop Sci., 63(1) (1), 140 - 141, Japanese

• バイオスティミュラントハンドブック: 植物の生理活性プロセスから資材開発、適用事例まで

  山内 靖雄, 須藤 修, 和田 哲夫, 和田, 哲夫 (pub. 2022)

  Contributor, 高CO2環境が植物生理に与える影響, エヌ・ティー・エス, Apr. 2022, Japanese, ISBN: 4860437691

• Improving Photosynthetic CO2 Fixation by Introducing C4-Like Rubisco into a C3 Plant, Rice

  Fukayama Hiroshi

  2nd Asia-Oceania International Congress on Photosynthesis, Sep. 2024, English

  [Invited]

  Invited oral presentation


• SNORKELs and Deepwater Response in the African Cultivated Rice Oryza glaberrima

  Quanshu Luo, Misaki Nakazawa, Daisuke, Sasayama, Tomoko Hatanaka, Hiroshi Fukayama, Tetsushi Azuma

  10th Asian Crop Science Association Conference, Sep. 2021


• Adaptive Responses to Flood in Wild Rice Species with Various Genomes Other Than AA

  Daisuke Sasayama, Mayuko Niikawa, Tomoko, Hatanaka, Hiroshi Fukayama, Tetsushi Azuma

  10th Asian Crop Science Association Conference, Sep. 2021


• Highly Active Vernonia galamensis DGAT1 Can Effectively Increase Oil Levels in Yeast, Soybean and Arabidopsis

  Tomoko Hatanaka, Yoshiki Tomita, Choi-Wing Chau, Honoka Ito, Daisuke Sasayama, Hiroshi Fukayama, Tetsushi Azuma, David F. Hildebrand

  10th Asian Crop Science Association Conference, Sep. 2021


• A Challenge for the Improvement of Photosynthetic Capacity by the Introduction of C4 -Like Rubisco in Rice

  Hiroshi Fukayama, Keita Shiomi, Yuri Taketani, Hiroki Yoshikawa, Daisuke Sasayama, Tomoko Hatanaka, Tetsushi Azuma, Takuya Yoshizawa, Shun-Ichi Tanaka, Hiroyoshi Matsumura

  10th Asian Crop Science Association Conference, Sep. 2021


• CO2 -Responsive CCT Protein Interacts with 14-3-3 Proteins and Regulates the Expression of Starch Synthesis-Related Genes

  Fumihiro Miyagawa, Naoki Shibatani, Aiko Koudou, Daisuke Sasayama, Tomoko Hatanaka, Tetsushi Azuma, Hiroshi Fukayama

  10th Asian Crop Science Association Conference, Sep. 2021

  Poster presentation


• Vernonia galamensis DGAT1 can increase Arabidopsis seed oil level more than other DGAT1s

  HATANAKA TOMOKO, 冨田 典希, 周 采穎, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI, DAVID F. HILDEBRAND

  第31回植物脂質シンポジウム, Nov. 2018, Japanese, 高知大学, Domestic conference

  Poster presentation


• Vernonia galamensis DGAT1 can increase Arabidopsis seed oil content more than other DGAT1s

  HATANAKA TOMOKO, YOSHIKI TOMITA, CHOI-WING CHAU, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI, DAVID F. HILDEBRAND

  The 14th International Symposium on Biocatalysis and Agricultural Biotechnology, Oct. 2018, English, Kuala Lumpur, Malaysia, International conference

  Poster presentation


• Analysis of Floating Response of South American Wild Rice Oryza glumaepatula

  NIIKAWA MAYUKO, ENDO HIROAKI, SASAYAMA DAISUKE, HATANAKA TOMOKO, FUKAYAMA HIROSHI, AZUMA TETSUSHI

  日本作物学会第246回講演会, Sep. 2018, Japanese, 北海道大学農学部, Domestic conference

  Poster presentation


• Structure-Function Relationship of Hybrid Rubisco Consisting of Sorghum RbcS and Rice RbcL

  SHIOMI KEITA, SASAYAMA DAISUKE, HATANAKA TOMOKO, AZUMA TETSUSHI, YOSHIKAWA HIROKI, MATSUMURA HIROYOSHI, FUKAYAMA HIROSHI

  日本作物学会第246回講演会, Sep. 2018, Japanese, 北海道大学農学部, Domestic conference

  Oral presentation


• Growth response of the African cultivated rice Oryza glaberrima and its wild ancestor Oryza barthii to flooding

  Luo Quanshu, NAKAZAWA MISAKI, SASAYAMA DAISUKE, HATANAKA TOMOKO, FUKAYAMA HIROSHI, AZUMA TETSUSHI

  日本作物学会第246回講演会, Sep. 2018, Japanese, 北海道大学農学部, Domestic conference

  Poster presentation


• Highly active Vernonia galamensis DGAT1 can effectively increase oil levels in yeast, soybean and Arabidopsis

  HATANAKA TOMOKO, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI, MATSUOKA DAISUKE, TOMITA YOSHIKI, CHAU CHOI-WING, MIYASHITA WAKANA, SERSON WILLIAM, LI RUNZHI, ARMSTRONG PAUL, YU KESHUN, PFEIFFER TODD, LI XI-LE, HILDEBRAND DAVID

  The 23rd International Symposium on Plant Lipids, Jul. 2018, English, Yokohama, Japan, International conference

  Poster presentation


• アフリカイネ Oryza glaberrima とその祖先種 O. barthii の洪水に対する応答

  羅 全淑, 中澤 美沙希, SASAYAMA DAISUKE, HATANAKA TOMOKO, FUKAYAMA HIROSHI, AZUMA TETSUSHI

  近畿作物・育種研究会 第185回例会, Jun. 2018, Japanese, Domestic conference

  Oral presentation


• バレイショにおけるイネCRCT遺伝子のオーソログの解析

  林龍之介, 横山音, 津釜大輔, 森田隆太郎, FUKAYAMA HIROSHI, 藤野介延

  日本作物学会講演会, 2018, Japanese, Domestic conference

  Poster presentation


• シロイヌナズナにおける CRCT オーソログの機能解析

  萩原輝, 中馬望, MATSUOKA DAISUKE, SASAYAMA DAISUKE, HATANAKA TOMOKO, AZUMA TETSUSHI, FUKAYAMA HIROSHI

  日本作物学会講演会, 2018, Japanese, Domestic conference

  Poster presentation


• イネの Rubisco activase 様タンパク質 Rca2 は Rubisco activase の機能を阻害する

  水本晃那, 小林亜希子, 山内靖雄, MATSUOKA DAISUKE, SASAYAMA DAISUKE, HATANAKA TOMOKO, AZUMA TETSUSHI, FUKAYAMA HIROSHI

  日本作物学会講演会, 2018, Japanese, Domestic conference

  Poster presentation


• イネRubisco activase様タンパク質OsRca2はRubiscoの活性化を阻害する

  水本晃那, 小林亜希子, MATSUOKA DAISUKE, YAMAUCHI YASUO, SASAYAMA DAISUKE, HATANAKA TOMOKO, AZUMA TETSUSHI, FUKAYAMA HIROSHI

  日本光合成学会講演会, 2018, Japanese, Domestic conference

  Poster presentation


• Introduction of C4-like enzymic properties and nitrogen distribution of Rubisco into C3 plant, rice

  FUKAYAMA HIROSHI, 山本滉樹人, 塩見慶太, 小林則之, 吉川大貴, 谷口洋二郎, 松村浩由

  日本植物生理学会年会, 2018, English, Domestic conference

  Nominated symposium


• Functional analysis of Rubisco small subunit, OsRbcS1, that is not expressed in photosynthetic organs in rice

  小林 則之, 森田 耕一, SASAYAMA DAISUKE, HATANAKA TOMOKO, AZUMA TETSUSHI, FUKAYAMA HIROSHI

  International Symposium on Photosynthesis and Chloroplast Biogenesis, 2018, English, Domestic conference

  Poster presentation


• C4植物の Rubisco 小サブユニットを高発現する形質転換イネの Rubisco 酵素特性

  小原崇, SASAYAMA DAISUKE, HATANAKA TOMOKO, AZUMA TETSUSHI, FUKAYAMA HIROSHI

  日本作物学会講演会, 2018, Japanese, Domestic conference

  Poster presentation


• Highly active Vernonia galamensis DGAT1 can effectively increase seed oil levels in soybean and Arabidopsis

  HATANAKA TOMOKO, TOMITA YOSHIKI, Chau, Choi-Wing, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI, William Serson, Runzhi Li, Paul Armstrong, Keshun Yu, Todd Pfeiffer, Xi-Le Li, David F. Hildebrand

  The 7th Asian Symposium on Plant Lipid, Nov. 2017, English, Taipei, Taiwan, International conference

  Poster presentation


• 浮稲性遺伝子と冠水耐性遺伝子を併せ持つイネ品種の深水成長応答と関連遺伝子の発現

  尾江 俊祐, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, HATANAKA TOMOKO, AZUMA TETSUSHI

  日本作物学会第244回講演会, Sep. 2017, Japanese, 岐阜大学, Domestic conference

  Poster presentation


• 南米野生イネOryza glumaepatulaの深水に対する応答とSNORKEL遺伝子の多様性

  遠藤 宏朗, 新川 麻由子, SASAYAMA DAISUKE, HATANAKA TOMOKO, FUKAYAMA HIROSHI, AZUMA TETSUSHI

  日本作物学会第244回講演会, Sep. 2017, Japanese, 岐阜大学, Domestic conference

  Poster presentation


• 深水環境下において浮稲節間の伸長を誘導する新規因子の探索

  冨田 一嘉, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, HATANAKA TOMOKO, AZUMA TETSUSHI

  日本作物学会第244回講演会, Sep. 2017, Japanese, 岐阜大学, Domestic conference

  Poster presentation


• Does Vernonia DGAT1 show a higher activity than other DGAT1s in Arabidopsis seed?

  HATANAKA TOMOKO, TOMITA YOSHIKI, CHAU CHOI-WING, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI, David F. Hildebrand

  日本作物学会 第244回講演会, Sep. 2017, Japanese, 岐阜大学, Domestic conference

  Poster presentation


• シロイヌナズナにおけるベルノニアDGAT1の働き

  HATANAKA TOMOKO, TOMITA YOSHIKI, CHAU CHOI-WING, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI, David F. Hildebrand

  第30回植物脂質シンポジウム, Sep. 2017, Japanese, お茶の水大学, Domestic conference

  Poster presentation


• Vernonia DGAT1 can increase Arabidopsis seed oil content more than other DGAT1s

  HATANAKA TOMOKO, TOMITA YSHIKI, CHAU CHOI-WING, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI, HILDEBRAND, D. F

  Plant Biology 2017, Jun. 2017, English, Honolulu, Hawaii, USA, Plant oils represent one of the world’s most important renewable resources. In addition to extensive food uses, vegetable oils also have value as renewable chemical derivatives and fuels, such as biodiesel. Worldwide production of plant oil has been increasing. Triacylglycerols (TAGs) are the major component of plant storage lipid. Acyl-CoA:diacylglycerol acyltransferase (DGAT), International conference

  Poster presentation


• マイクロアレイ法によるOsRbcS1発現抑制イネの遺伝子発現プロファイリング

  森田 耕一, SASAYAMA DAISUKE, AZUMA TETSUSHI, FUKAYAMA HIROSHI

  日本作物学会講演会, 2017, Japanese, Domestic conference

  Poster presentation


• デンプン合成制御因子CRCTが稲わらを用いたバイオエタノール生産に及ぼす効果

  森田 隆太郎, 寺村 浩, OGINO CHIAKI, KONDO AKIHIKO, FUKAYAMA HIROSHI

  日本作物学会講演会, 2017, Japanese, Domestic conference

  Poster presentation


• ソルガム高活性型Rubiscoの小サブユニットを利用したイネの光合成能力の改良

  塩見慶太, 山本滉樹人, HATANAKA TOMOKO, SASAYAMA DAISUKE, AZUMA TETSUSHI, FUKAYAMA HIROSHI

  農芸化学会関西支部会, 2017, Japanese, Domestic conference

  Oral presentation


• イネにおけるRubisco activase様タンパク質OsRca2の機能解析

  水本晃那, 小林亜希子, HATANAKA TOMOKO, SASAYAMA DAISUKE, AZUMA TETSUSHI, FUKAYAMA HIROSHI

  農芸化学会関西支部会, 2017, Japanese, Domestic conference

  Oral presentation


• CO2応答性CCTタンパク質CRCTはイネ栄養器官のデンプン合成制御に働く

  森田 隆太郎, FUKAYAMA HIROSHI

  日本作物学会講演会, 2017, Japanese, Domestic conference

  [Invited]

  Nominated symposium


• 浮稲性遺伝子と冠水耐性遺伝子を併せ持つイネ系統の深水伸長応答

  OE SHUNSUKE, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI

  日本作物学会 第242回講演会, Sep. 2016, Japanese, 龍谷大学瀬田キャンパス, Domestic conference

  Poster presentation


• 水田雑草オモダカとウリカワの塊茎萌芽期の節間伸長の酸素欠乏および二酸化炭素による促進

  SASAYAMA DAISUKE, FUKAYAMA HIROSHI, ITOH KAZUYUKI, AZUMA TETSUSHI

  日本作物学会 第242回講演会, Sep. 2016, Japanese, 龍谷大学瀬田キャンパス, Domestic conference

  Oral presentation


• エチレンとジベレリンが誘導する浮稲節間の伸長促進に及ぼす重力方向と過重力の影響

  NAKATANI YUMI, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI

  日本作物学会 第242回講演会, Sep. 2016, Japanese, 龍谷大学瀬田キャンパス, Domestic conference

  Poster presentation


• 浮稲性遺伝子と冠水耐性遺伝子を併せ持つイネ系統の生理特性

  OE SHUNSUKE, SASAYAMA DAISUKE, FUKAYAMA HIROSHI, AZUMA TETSUSHI

  近畿作物・育種研究会 第181回例会, May 2016, Japanese, Domestic conference

  Oral presentation


• 多年生水田雑草オモダカとウリカワの節間伸長はそれぞれ酸素欠乏と二酸化炭素によって促進される

  SASAYAMA DAISUKE, FUKAYAMA HIROSHI, ITOH KAZUYUKI, AZUMA TETSUSHI

  近畿作物・育種研究会 第181回例会, May 2016, Japanese, 滋賀県立大学 サテライト教室, Domestic conference

  Oral presentation


• Enhancement of storage lipid biosynthesis in higher plants

  TOMITA YOSHIKI, KIMURA MADOKA, FUKAYAMA HIROSHI, MISOO SHUJI, HATANAKA TOMOKO

  日本作物学会第241回講演会, Mar. 2016, Japanese, Domestic conference

  Poster presentation


• 高CO2条件で育成したソルガムRbcS高発現・イネRbcS発現抑制2重形質転換イネの生理特性

  山本 滉樹人, 迫田 和馬, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本作物学会講演会, Mar. 2016, Japanese, Domestic conference

  Poster presentation


• デンプン合成関連酵素の発現制御に働くCRCTは葉鞘のデンプンの鎖長分布パターンに影響を与える

  森田 隆太郎, クロフツ 尚子, HATANAKA TOMOKO, MISOO SHUJI, 藤田 直子, FUKAYAMA HIROSHI

  日本作物学会講演会, Mar. 2016, Japanese, Domestic conference

  Oral presentation


• シロイヌナズナにおけるCRCTオーソログの発現解析

  萩原 輝, 中馬 望, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本作物学会講演会, Mar. 2016, Japanese, Domestic conference

  Poster presentation


• イネ葉身の発達・老化過程におけるOsRca2 含量の変化

  水本 晃那, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本作物学会講演会, Mar. 2016, Japanese, Domestic conference

  Poster presentation


• OsRbcS1の代謝機能：植物におけるOsRbcS1様RbcSの発現解析とOsRbcS1発現抑制イネのメタボローム解析

  森田 耕一, HATANAKA TOMOKO, MISOO SHUJI, 草野 都, FUKAYAMA HIROSHI

  日本作物学会講演会, Mar. 2016, Japanese, Domestic conference

  Poster presentation


• CO2 Responsive CCT protein alters the chain-length distribution of starch from leaf sheath in rice by regulating the expression of multiple starch synthesis related enzymes

  森田 隆太郎, クロフツ尚子, HATANAKA TOMOKO, MISOO SHUJI, 藤田 直子, FUKAYAMA HIROSHI

  日本植物生理学会年会, Mar. 2016, Japanese, Domestic conference

  Poster presentation


• Starch Content in Leaf Sheath Controlled by CO2 Responsive CCT Protein is a potential Determinant of Photosynthetic Capacity in Rice Grown under Elevated CO2 Condition

  Ryutaro Morita, Tomoko Hatanaka, Shuji Misoo, Hiroshi Fukayama

  The 17th International Congress on Photosynthesis Research, 2016, English, International conference

  Poster presentation


• Functional analysis of OsRbcS1, a non-photosynthetic small subunit that increases the catalytic turnover rate of Rubisco in rice

  Koichi Morita, Tomoko Hatanaka, Shuji Misoo, Hiroshi Fukayama

  The 17th International Congress on Photosynthesis Research, 2016, English, International conference

  Poster presentation


• Engineering Rubisco and starch synthesis to enhance photosynthesis under elevated CO2

  FUKAYAMA HIROSHI

  The 2015 Tokyo Whole Plant Photosynthesis Workshop, May 2015, English, Domestic conference

  Invited oral presentation


• Sink Capacity of Leaf Sheath Regulated by CRCT Affects the Photosynthetic Rate of Leaf Blade in Rice Grown under Elevated CO2 Condition.

  森田 隆太郎, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本植物生理学会年会, Mar. 2015, Japanese, Domestic conference

  Oral presentation


• Responsive CCT Protein Ortholog in Arabidopsis Controls the Starch Accumulation in Leaves.

  中馬 望, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本植物生理学会年会, Mar. 2015, Japanese, Domestic conference

  Poster presentation


• Functional analysis of Rubisco activase-like protein, OsRca2 in rice.

  小林 亜希子, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本植物生理学会年会, Mar. 2015, Japanese, Domestic conference

  Oral presentation


• CO2 Responsive CCT Protein, CRCT Is a Positive Regulator of Starch Synthesis in Vegetative Organ in Rice.

  高堂 愛子, 森田 隆太郎, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本植物生理学会年会, Mar. 2015, Japanese, Domestic conference

  Poster presentation


• A rice MAP3K, Os11g45280, controls plant growth concerning tiller formation

  山村 優太, FUKAYAMA HIROSHI, MATSUOKA DAISUKE, NANMORI TAKASHI

  第５６回日本植物生理学会年会, Mar. 2015, Japanese, Domestic conference

  Poster presentation


• Rubisco activase様タンパク質OsRca2の光合成における機能

  FUKAYAMA HIROSHI, 小林 亜希子, 水本晃那, HATANAKA TOMOKO, MISOO SHUJI

  日本作物学会講演会, 2015, Japanese, Domestic conference

  Oral presentation


• 耐塩性インディカイネ交雑由来倍加半数体系統の継続的耐塩性評価

  ITAKURA SHUNSUKE, FUKAYAMA HIROSHI, HATANAKA TOMOKO, MISOO SHUJI

  日本作物学会, Sep. 2014, Japanese, 愛媛大学, Domestic conference

  Oral presentation


• デンプン合成の制御因子CRCTの発現量がイネの生育と光合成に与える影響

  森田 隆太郎, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本作物学会講演会, Sep. 2014, Japanese, Domestic conference

  Poster presentation


• イネ高活性型Rubisco小サブユニット遺伝子OsRbcS1のオーソログの発現解析

  森田 耕一, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本作物学会講演会, Sep. 2014, Japanese, Domestic conference

  Poster presentation


• ダイズ体細胞胚長期懸濁培養におけるマルトースの効果に関する一考察

  HATANAKA TOMOKO, KIHARA YUKI, SAKAI TSUNEMITSU, SETA SATOMI, FUKAYAMA HIROSHI, MISOO SHUJI

  第177回 近畿作物・育種研究会例会, Jul. 2014, Japanese, 神戸大学農学部, Domestic conference

  Oral presentation


• 高CO2条件下で発現促進されるCCTタンパク質CRCTはイネの栄養器官におけるデンプン合成の調節因子である

  森田 隆太郎, 杉野 充保, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本作物学会, Mar. 2014, Japanese, 千葉大学, Domestic conference

  Oral presentation


• 寒地型イネ科牧草チモシーのRbcSを高発現する形質転換イネの光合成特性．

  古賀 敦士, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本作物学会, Mar. 2014, Japanese, 千葉大学, Domestic conference

  Oral presentation


• ソルガム高活性型Rubiscoの小サブユニットを高発現しRNAi法でRubisco含量を減少させた二重形質転換イネの光合成特性．

  迫田 和馬, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本作物学会, Mar. 2014, Japanese, 千葉大学, Domestic conference

  Oral presentation


• イネ高活性型Rubisco小サブユニットOsRbcS1の機能解析

  森田 耕一, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本作物学会, Mar. 2014, Japanese, 千葉大学, Domestic conference

  Oral presentation


• イネのPASドメインを有するMAP3Kの機能について

  TAKAOKA SHOHEI, FUKAYAMA HIROSHI, MATSUOKA DAISUKE, NANMORI TAKASHI

  日本農芸化学会2014年度大会, Mar. 2014, Japanese, Domestic conference

  Oral presentation


• C3植物のC4化：Rubiscoだけでも役に立つ？

  FUKAYAMA HIROSHI

  日本植物学会, 2014, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• 耐塩性イネ品種PokkaliのF1倍加半数体系統群における幼苗期耐塩性指標の評価

  梅嵜友理香, 西村幸一郎, FUKAYAMA HIROSHI, HATANAKA TOMOKO, MISOO SHUJI

  日本作物学会, 2013, Japanese, Domestic conference

  Oral presentation


• 高CO2条件で発現促進される新規CCTドメインタンパク質CRCTは分げつ角度と基本代謝酵素の発現調節に働く

  森田隆太郎, 杉野充保, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本作物学会, 2013, Japanese, Domestic conference

  Oral presentation


• ソルガム高活性型Rubiscoの小サブユニットを高発現しRNAi法等でRubisco量を減少させた形質転換イネの生理特性

  FUKAYAMA HIROSHI, 石川智恵, HATANAKA TOMOKO, MISOO SHUJI

  日本作物学会, 2013, Japanese, Domestic conference

  Oral presentation


• イネにおいて葉身で発現しない新規Rubisco小サブユニットOsRbcS1の機能解析

  森田耕一, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本作物学会, 2013, Japanese, Domestic conference

  Oral presentation


• Rubisco activaseはRubisco生合成を阻害することによりRubiscoの発現を抑制する

  上口千晶, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本作物学会, 2013, Japanese, Domestic conference

  Oral presentation


• 幼苗期におけるインディカイネの交雑親と倍加半数体系統群の耐塩性比較

  西村 幸一郎, FUKAYAMA HIROSHI, HATANAKA TOMOKO, MISOO SHUJI

  日本作物学会, 2012, Japanese, Domestic conference

  Oral presentation


• 高CO2条件で発現促進される受容体型キナーゼOsTPK1の機能解析

  廣瀬 由依, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本作物学会, 2012, Japanese, Domestic conference

  Oral presentation


• 高CO2 環境に適したRubisco の導入によるイネの光合成能力の改良

  FUKAYAMA HIROSHI

  本光合成学会 公開シンポジウム「植物とCO2」, 2012, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• イネにおいて高CO2条件下で発現促進される新規CCTタンパク質OsCCT1の機能解析

  FUKAYAMA HIROSHI, 森田隆太郎, 杉野充保, HATANAKA TOMOKO, MISOO SHUJI

  日本植物生理学会, 2012, Japanese, Domestic conference

  Oral presentation


• 新規なCCTドメインタンパク質OsCCT1は高CO2環境への代謝的・形態的応答に関係している．

  杉野充保, FUKAYAMA HIROSHI, 酒井英光, HATANAKA TOMOKO, MISOO SHUJI

  日本作物学会, 2011, Japanese, Domestic conference

  Oral presentation


• イネにおける葉緑体型ホスホグリセリン酸ムターゼOsPGMd2の機能解析

  加藤祐樹, FUKAYAMA HIROSHI, 大河浩, 谷口洋二郎, 増本千都, 宮尾光恵, HATANAKA TOMOKO, MISOO SHUJI

  日本作物学会, 2011, Japanese, Domestic conference

  Oral presentation


• Rubisco activaseの高発現はRubisco含量の減少により光合成速度を低下させる

  FUKAYAMA HIROSHI, 上口千晶, 西川薫, 石川智恵, HATANAKA TOMOKO, MISOO SHUJI

  日本植物生理学会, 2011, Japanese, Domestic conference

  Oral presentation


• ソルガムRbcS の高発現は形質転換イネにおけるRubisco の反応回転速度を増加させる

  石川智恵, HATANAKA TOMOKO, MISOO SHUJI, FUKAYAMA HIROSHI

  日本植物生理学会年会, 2010, Japanese, Domestic conference

  Oral presentation


• イネにおけるプラスチド局在型エノラーゼ遺伝子の機能解析

  FUKAYAMA HIROSHI, 加藤祐樹, 新谷翔太, 谷口洋二郎, 増本千都, HATANAKA TOMOKO, MISOO SHUJI, 宮尾光恵, 大河浩

  日本作物学会, 2010, Japanese, Domestic conference

  Oral presentation


• Chimeric Incorporation of Sorghum RbcS Increases the Catalytic Turnover Rate of Rubisco in Transgenic Rice Plants

  Chie Ishikawa, Tomoko Hatanaka, Shuji Misoo, Hiroshi Fukayama

  The 15th International Congress of Photosynthesis, 2010, English, International conference

  Oral presentation


• オオムギRubisco activaseの高発現がイネの光合成特性と葉内成分含量におよぼす効果

  FUKAYAMA HIROSHI, 西川 薫, HATANAKA TOMOKO, MISOO SHUJI

  日本作物学会大会, 2009, Japanese, Domestic conference

  Oral presentation


• 油料作物Jatropha curcasの組織培養による増殖と脂質定量

  栗本 温子, HATANAKA TOMOKO, FUKAYAMA HIROSHI, MISOO SHUJI, 三本木 一夫

  日本作物学会大会, 2008, Japanese, Domestic conference

  Poster presentation


• 油料作物Jatropha curcasの脂質含量と組織培養による増殖

  栗本 温子, HATANAKA TOMOKO, FUKAYAMA HIROSHI, MISOO SHUJI, 三本木 一夫

  近畿作物・育種研究会例会, 2008, Japanese, 神戸大学, Domestic conference

  Oral presentation


• 丹波黒大豆‘兵系黒３号’の成熟培養における，糖とアミノ酸の効果

  木原 由貴, HATANAKA TOMOKO, FUKAYAMA HIROSHI, MISOO SHUJI

  日本作物学会大会, 2008, Japanese, Domestic conference

  Poster presentation


• 開放系大気CO2 濃度増加条件（FACE)で育成したイネの遺伝子発現プロファイリング

  FUKAYAMA HIROSHI, 福田 琢哉, 長谷川 利拡, 宮尾 光恵, HATANAKA TOMOKO, MISOO SHUJI

  近畿作物・育種研究会例会, 2008, Japanese, 神戸大学, Domestic conference

  Oral presentation


• イネの光合成能力改良に向けたイネ科植物内における高Kcat Rubiscoの探索

  FUKAYAMA HIROSHI, 石川 智恵, HATANAKA TOMOKO, MISOO SHUJI

  日本作物学会大会, 2008, Japanese, Domestic conference

  Oral presentation


• Ribulose-1,5-bisphosphate carboxylase/oxygenase activaseを過剰発現させた形質転換イネの光合成特性

  増本 千都, 山田 健太郎, 加藤 伸明, FUKAYAMA HIROSHI, HATANAKA TOMOKO, UCHIDA NAOTSUGU

  日本作物学会大会, 2007, Japanese, Domestic conference

  Oral presentation


• Ribulose-1,5-bisphosphate carboxylase/oxygenase acitivase (Rubisco activase)含量を減少させた形質転換イネの光強度に対する光合成反応

  増本 千都, HATANAKA TOMOKO, FUKAYAMA HIROSHI, UCHIDA NAOTSUGU

  日本作物学会大会, 2006, Japanese, Domestic conference

  Poster presentation

• 日本光合成研究会


• 日本植物生理学会


• 日本作物学会

• Production of plants with high photosynthetic capacity by chloroplast genome editing technology and elucidation of its mechanism

  矢守 航, 深山 浩, 松村 浩由

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Transformative Research Areas (A), The University of Tokyo, Apr. 2024 - Mar. 2029


• イネ科作物の栄養器官におけるデンプン合成制御メカニズムの解明

  深山 浩

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2023 - 31 Mar. 2026

  「CRCTの分子機能の解明」に関しては，CRCTが組織間を移動している可能性を検証するために，維管束特異的に発現するSUT1プロモーターとRPP16プロモーター，緑色組織特異的に発現するCabプロモーター，構成的に発現するActinプロモーターで制御したCRCTをCRCT欠損変異体に導入した．得られた形質転換イネのデンプン合成関連遺伝子の発現，デンプン蓄積量の解析を行ったところ，SUT1プロモーター，Cabプロモーター，ActinプロモーターでCRCTを制御した場合は，デンプン合成関連遺伝子の発現，デンプン蓄積量が増加した．これらの結果から，CRCTが組織間を移動することが強く示唆された．また，CRCTの14-3-3タンパク質結合サイト（リン酸化サイト）である217番目のセリンをアラニンに置換し，14-3-3タンパク質と結合できない変異型CRCT（CRCT-S217A）をCRCT欠損変異体に導入した形質転換イネ（OX-CRCT-S217A）を作出した． 「CRCTの作用のイネ品種間差の解析」に関しては，イネ6品種（日本晴，コシヒカリ，ハバタキ，タカナリ，モミロマン，リーフスター）に関して葉身を使ってスクロース処理を行い，デンプン合成関連酵素の発現解析を行った．CRCTに対するデンプン合成関連遺伝子の発現誘導性は，ハバタキ，タカナリで低い傾向が見られた．この結果は，イネの生育段階で比較した結果と一致し，CRCTの作用に品種間差が存在することが示唆された． 「イネ以外の単子葉植物におけるCRCTの機能解析」に関しては，トウモロコシではなくソルガムを用いて進めることとした．ソルガムCRCTの完全長cDNAをイネActinプロモーターに連結したコンストラクトを作成し，アグロバクテリウム法でソルガム未熟胚に遺伝子導入を行っている．


• Rubiscoの触媒特性改変による光合成能力の改良と構造機能相関の解明

  深山 浩, 松村 浩由

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Kobe University, Apr. 2020 - Mar. 2023

  形質転換の際に生じた可能性のある体細胞変異の影響を排除するために，非形質転換イネを花粉親としてCSS系統の戻し交配を2回行った．得られた後代を自殖させて育成したが，稔実率は回復しなかった．これらの結果から，稔実率を低下させる要因が導入したソルガムRbcS遺伝子と連鎖している可能性が考えられた．よって，これまでと異なるソルガムRbcS高発現イネ系統にCSS系統を交配し，新たなCSS系統の作出に着手した． これまでソルガムRbcSの高発現には，Cabプロモーターを用いてきた．生育不良が用いたプロモーターによる可能性があり，新たにOsRbcS3プロモーターを用いてソルガムRbcSを高発現する系統の作出を行った． ソルガムRbcSの102番目のLeu (イネではIle) がRubiscoの触媒速度を高める効果を持つことが示唆された．この仮説を検証するために，ソルガムRbcSの102番目のLeuがIle (ソルガムL102I)，イネRbcSの102番目のIleがLeu (イネI102L) となった変異型RbcSを発現する形質転換イネをした．さらに，CSS系統と交配して得たF1を自殖させることで，野生型RbcSを欠損した系統を作出した． ソルガム以外のC4植物のRbcSについては，ネピアグラス，ギニアグラスについては完全置換系統の作成が完了した．トウモロコシについては高発現系統の作出を行った． 光合成器官で発現しないイネOsRbcS1は，イネRubiscoの触媒速度を増加させ，触媒反応の至適ｐHを酸性化させる効果がある．OsRbcS1の102番目のアミノ酸はIleである．OsRbcS1高発現イネについてイネRbcSをRNAiで発現抑制した系統を作成済みでありRubiscoのX線結晶構造を1.9Åの解像度で決定した．光合成で働くイネRubiscoとOsRbcS1が組込まれたイネRubiscoの構造は非常に類似しているが，我々が着目している102番目のアミノ酸の近傍の106番目のAspがOsRbcS1ではSerとなっており，触媒速度に関係している可能性が示唆された．


• Unraveling of the control mechanism of starch synthesis in vegetative organs of plants

  Hyogo Science and Technology Association, Grant-in-aid from Hyogo Science and Technology Association, Apr. 2019 - Mar. 2020, Principal investigator


• RubiscoのC4化によるイネの光合成能力の改良

  深山 浩

  科学研究費補助金／基盤研究(B), Apr. 2015 - Mar. 2019, Principal investigator

  Competitive research funding


• イネが持つ新規Ｒｕｂｉｓｃｏ小サブユニットＯｓＲｂｃＳ１の機能解析

  深山 浩

  科学研究費補助金／基盤研究(C), Apr. 2012 - Mar. 2015, Principal investigator

  Competitive research funding


• イネ葉において高ＣＯ２条件下で発現促進されるＯｓＣＣＴ１とＯｓＴＰＫ１の機能解析

  深山 浩

  日本学術振興会, 科学研究費補助金／新学術領域研究, 新学術領域研究(研究領域提案型), 神戸大学, 2010 - 2011, Principal investigator

  高CO_2条件下で育成したイネ葉において顕著に発現促進されるOsCCT1とOsTPK1について高発現,ノックダウン形質転換イネを作出し生理機能の解析を行った. これまでの研究で,OsCCT1は株の開帳度に関係することが示唆された.本年度は大気条件と高CO_2条件で育成した場合の第2葉分げつと主稈の間の角度について解析した.いずれのCO_2濃度においても分げつ角度は高発現形質転換イネで増加した.分げつ角度は光強度や生育CO_2濃度と正に相関することが明らかとなり,光合成が促進され,植物体内の糖濃度が高いと分げつの開帳度が増すものと考えられた.また,その他の生育特性に関しては分げつ数が高発現イネで少なく,ノックダウンイネで多くなる傾向が認められた.このような分げつ数への効果は高CO_2で育成した場合も認められた.光合成速度,気孔伝導度は生育CO_2濃度によらず,両方の形質転換イネで僅かに低下する傾向が認められた.高CO_2条件で育成した場合,両方の形質転換イネで乾物重が低下する傾向が認められ,その低下は高発現形質転換イネでより顕著であった.以上の結果,OsCCT1は分げつの角度や数を決める上で重要な働きを担っていること,高CO_2条件での生育や光合成においてOsCCT1の適切な発現が重要であることが示唆された. OsTPK1の高発現,ノックダウン形質転換イネについても生理解析を行った.OsTPK1の発現量を変化させても光合成速度,生育特性はほとんど影響を受けなかった.しかし,両方の形質転換イネで葉身,葉鞘の乾物重が低下することがわかった.マイクロアレイを行ったところ,高発現,ノックダウン形質転換イネは非常に類似した発現プロファイルを示した.この結果から,高発現はドミナントネガティブになっている可能性が考えられた.両方の形質転換イネにおいて複数のエクスパンシン遺伝子が顕著に発現抑制されていた.高CO_2処理をするとイネは葉身が小さくなることが知られていることから,OsTPK1はそのような葉の形態的な応答に関係している重要な遺伝子ではないかと考えられた.

  Competitive research funding


• Improvement of photosynthetic efficiency by introduction of high kcat Rubisco from Poaseae.

  FUKAYAMA Hiroshi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 2008 - 2011, Principal investigator

  The activity of Rubisco is a major limitation of photosynthetic CO_2 assimilation rate in C_3 plants. In order to find useful Rubisco for improvement of photosynthesis in rice under elevated CO_2, the catalytic turnover rate(k_) of Rubisco was analyzed in Poaceae including C_3 alpine plants, C_3 cold resistant plants and C_4 plants. Rubisco in these plants showed 1. 1-to 2. 8-fold higher k_ than that in rice. Among plants analyzed, Rubisco in Festuca ovina, Phleum pratense and Sorghum showed relatively high k_ to Kc. In our estimation, the expression of high k_ Rubisco of F. ovina and S. bicolor in rice could significantly enhance CO_2 assimilation rates under elevated CO_2. We introduced the small subunit(RbcS) of Sorghum and it was found that Sorghum RbcS significantly enhances k_ of Rubisco in transgenic rice(Oryza sativa). These results suggest that Rubisco in rice transformed with sorghum RbcS partially acquires the catalytic properties of sorghum Rubisco. Rubisco content in transgenic lines was significantly increased over wild type levels but Rubisco activation was slightly decreased. The expression of sorghum RbcS did not affect CO_2 assimilation rates under a range of CO_2 partial pressures. The J_/V_ ratio was significantly lower in transgenic line compared to the nontransgenic plants. These observations suggest that the capacity of electron transport is not sufficient to support the increased Rubisco capacity in transgenic rice. Although the photosynthetic rate was not enhanced, the strategy presented in this study opens the way to engineering Rubisco for improvement of photosynthesis and productivity in the future.

  Competitive research funding


• FUNCTIONAL ANALYSIS OF PHOSPHO ENOLPYRUVATE CARBOXYLASE TARGETED TO THE CHLOROPLAST

  TOKUTOMI Mitsue, FUKAYAMA Hiroshi, MASUMOTO Chisato, MIYAZAWA Shin-ichi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), National Institute of Agrobiological Sciences, 2006 - 2008

  イネ特有の代謝酵素である葉緑体局在性ホスホエノールピルビン酸カルボキシラーゼ(PEPC)は、無機窒素を有機物に取り込む際必要な炭素化合物(有機酸)を作る働きをもつこと、イネはこの酵素が関与する独自の代謝経路をもち、この経路が水田のような湛水環境での無機窒素の利用に重要な役割を担うことが明らかにされた。葉緑体局在性PEPCは、光合成で固定された炭素の代謝と無機窒素の取り込みという、生産性に関わるふたつの過程をつなぐ鍵酵素であり、本酵素の関与する代謝経路の全容を理解することによって、イネの生産性の決定要因が明らかにされるものと期待される。