Research activity information

• Potential of zoledronate for treating diffuse sclerosing osteomyelitis of the mandible in adult patients.

  Junya Kusumoto, Shungo Furudoi, Yumi Muraki, Moeka Warabi, Daisuke Takeda, Masaya Akashi

  INTRODUCTION: Diffuse sclerosing osteomyelitis (DSO) is a rare nonbacterial bone disease associated with recurrent pain and swelling, and its pathogenesis remains unknown. Despite the absence of an established treatment for DSO, bisphosphonates have recently been considered effective in managing this condition. However, the use of zoledronate is off-label, with limited reported cases. Therefore, this study aimed to investigate the effects of zoledronate on pain suppression in DSO. METHODS: This single-arm retrospective study evaluated adult patients diagnosed with mandibular DSO and treated with zoledronate. Patient demographics, pain suppression effect, recurrence, number of zoledronate administration, adverse reactions to zoledronate, and imaging findings were investigated. RESULTS: The study included 18 patients (median age of 59.5 years). Zoledronate effectively suppressed pain in all patients, with a median duration of effect onset of 1 day. Symptom recurrence was observed in 66.7% of patients, with a median time of 29 months from the first zoledronate administration to recurrence. Zoledronate was administered multiple times to 44.7% of patients. The median duration of response was 80 months for patients who experienced relief after a single administration of zoledronate compared to 32 months for those who received multiple administrations (p < 0.001). Adverse reactions, including flu-like symptoms, were observed in 72.2% of the patients, and no medication-related osteonecrosis of the jaw was observed during the follow-up period. CONCLUSIONS: Zoledronate is a relatively safe and effective treatment option for DSO of the mandible. Key Points • Zoledronate effectively suppresses pain in mandibular diffuse sclerosing osteomyelitis. • All condylar lesions recurred after zoledronate administration. • Patients with mandibular canal enlargement required multiple doses of zoledronate. • Adverse reactions to zoledronate administration were minor.

  Apr. 2025, Clinical rheumatology, English, International magazine

  Scientific journal


• Evaluation of Zoledronate for Pain Suppression in Diffuse Sclerosing Osteomyelitis of the Mandible: Protocol for a Prospective, Single-Arm Interventional Study.

  Junya Kusumoto, Shungo Furudoi, Daisuke Takeda, Megumi Kishimoto, Masaya Akashi

  INTRODUCTION: Diffuse sclerosing osteomyelitis (DSO) is a non-bacterial osteomyelitis that occurs infrequently and is characterized by recurrent severe pain, swelling of the mandible, and trismus. To date, no effective treatment has been established. Bisphosphonates have been suggested as an efficacious treatment for DSO; however, no prospective studies have examined the effect of bisphosphonate treatment in adults with DSO. This study aims to evaluate the effect of zoledronate on pain suppression in patients with DSO. METHODS: This is a single-center, exploratory, single-arm, prospective interventional study. The participants will include eight patients with DSO of the mandible. The primary endpoint is the change in pain levels before and after zoledronate administration. The secondary endpoints are assessing the efficacy of the clinical course, imaging changes, and safety monitoring. Patients will be enrolled if they are deemed eligible following screening tests and will receive only one 4.0 mg intravenous dose of zoledronate within seven days of enrollment. Study participants will be followed up at one, four, 12, 24, and 48 weeks post-dose or at discontinuation after zoledronate administration. CONCLUSION: This study is the first prospective trial to evaluate the effect of administering a single dose of zoledronate on pain suppression in patients with DSO. We believe this study will lead to the conclusion that zoledronate is an effective treatment for DSO.

  Mar. 2025, Cureus, 17(3) (3), e80739, English, International magazine

  Scientific journal


• Zymosan-Treated SKG Mice: Assessing Effects of Systemic Inflammation on the Temporomandibular Joint.

  Kaito Uryu, Yasumasa Kakei, Sho Sendo, Ikuko Goto, Kengo Akashi, Keisuke Nishimum, Tatsuya Shirai, Junya Hirota, Daisuke Takeda, Manabu Shigeoka, Akira Kimoto, Takumi Hasegawa, Jun Saegusa, Masaya Akashi

  OBJECTIVES: The effects of systemic inflammation on the temporomandibular joint (TMJ) are poorly understood. This study aimed to establish a mouse model to study the effects of systemic inflammation on the TMJ. MATERIALS AND METHODS: SKG mice, a BALB/c strain with spontaneous onset of rheumatoid arthritis-like symptoms due to a spontaneous point mutation (W163C) in the gene encoding the SH2 domain of ZAP-70, were treated with zymosan (β-1,3-glucan). Synovitis, bone erosion, and cartilage damage in the TMJ were evaluated using established scores for animal models of inflammatory arthritis. Myeloperoxidase-positive areas and numbers of tartrate-resistant acid phosphatase (TRAP)-positive cells were compared between naive and zymosan-treated SKG mice. Correlations between TMJ inflammation scores and clinical scores for extremities were also assessed. RESULTS: There were significant differences in TMJ inflammation scores, including synovitis, bone erosion, and cartilage damage, between naive and high-dose zymosan-treated mice. There were significant differences in myeloperoxidase-positive areas and numbers of TRAP-positive cells between naive and zymosan-treated mice. There were significant correlations between TMJ inflammation scores and clinical scores for extremities. CONCLUSIONS: Systemic administration of zymosan efficiently induces TMJ inflammation in SKG mice. Zymosan-treated SKG mice offer a useful tool to investigate the effects of systemic inflammation on the TMJ.

  Dec. 2024, Oral diseases, English, International magazine

  Scientific journal


• Dental dysplasia in childhood cancer survivors: a case series of permanent tooth abnormalities.

  Yumi Muraki, Atsushi Shioyasono, Mika Nishii, Daisuke Takeda, Junya Kusumoto, Masaya Akashi

  PURPOSE: This study was performed to investigate the association of dental dysplasia with childhood cancer. We examined the occurrence of agenesis, microdontia, and enamel changes of permanent teeth in pediatric cancer survivors. METHODS: Seventy-six patients with pediatric cancer and hematologic diseases were referred to our department for the first time from October 2005 to December 2019. Of these, 17 patients who presented for a checkup in our department fulfilled the study criteria and were analyzed. Clinical examinations and panoramic radiographs were performed to investigate dental dysplasia of permanent teeth. A total of 34 developmental abnormalities (18 missing teeth, 16 cases of microdontia) occurred in 5 patients. The patients' medical records were also analyzed to assess the relationships of specific types of dysplasia with the treatment duration and cumulative drug dose administered. RESULTS: All five pediatric cancer survivors had dental dysplasia; all five had tooth agenesis, and four had microdontia. All five patients were < 4 years of age and had undergone high-dose chemotherapy. CONCLUSION: In this study, childhood cancer survivors who received high-dose chemotherapy before age 4 years experienced the dental dysplasia, including tooth agenesis, microdontia. These findings highlight the need for careful dental monitoring and early intervention in pediatric patients undergoing high-dose chemotherapy. CLINICAL TRIAL NUMBER: This research was conducted in accordance with the Declaration of Helsinki and approved by the Medical Ethics Committee of the Medical University of Kobe (protocol code No. B230066, 1 September 2023).

  Nov. 2024, Oral and maxillofacial surgery, 29(1) (1), 9 - 9, English, International magazine

  Scientific journal


• Relationship between bone union and degree of bone marrow fibrosis at resection margins of advanced mandibular ORN.

  Hiroaki Ohori, Eiji Iwata, Chihiro Ichikawa, Manabu Shigeoka, Yoshiaki Tadokoro, Daisuke Takeda, Junya Kusumoto, Takumi Hasegawa, Masaya Akashi

  BACKGROUND: The pathological evaluation of cancellous bone at resection margins of mandibular osteoradionecrosis (ORN) has not been well elucidated. Here, we developed a unique classification system for evaluating the degree of bone marrow fibrosis, one of most common pathological features, in patients with mandibular ORN, based on which we investigated its relationship with treatment outcome. METHODS: This study included 15 patients who underwent mandibulectomy and free fibula osteocutaneous flap reconstruction. The extent of mandibulectomy was determined, with safety margins of approximately 10 mm from the apparent osteolytic areas on preoperative computed tomography image. Special staining was performed on thin sections from center of the osteolytic areas (medial area) and bilateral resection margins, and the degree of bone marrow fibrosis was evaluated and investigated its relationship with presence of bone union as a treatment outcome. RESULTS: The degree of bone marrow fibrosis of medial area was significantly higher than those of resection margins. Although most resection margins had collagen fibers which indicate severe fibrosis, all transferred fibula flaps achieved bone union. CONCLUSION: When mandibulectomy is performed with safety margins of approximately 10 mm from the apparent osteolytic areas, all transferred fibula flaps achieved bone union regardless of the degree of bone marrow fibrosis at resection margin. In other words, the association between severe bone marrow fibrosis at resection margins and treatment outcome was not seen. CLINICAL RELEVANCE: Setting safety margins of approximately 10 mm may achieve bone union, but further study is needed.

  Nov. 2024, Clinical oral investigations, 28(11) (11), 626 - 626, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 下顎びまん性硬化性骨髄炎に対してゾレドロン酸は有効な治療薬となりうるか?

  楠元 順哉, 古土井 春吾, 村木 友美, 武田 大介, 明石 昌也

  (NPO)日本口腔科学会, Sep. 2024, 日本口腔科学会雑誌, 73(2) (2), 195 - 195, Japanese


• ヒト下歯槽神経のバリア制御に関連する分子の解析

  平岡 佑二郎, 松村 恵実, 筧 康正, 武田 大介, 重岡 学, 木本 明, 長谷川 巧実, 明石 昌也

  (NPO)日本口腔科学会, Sep. 2024, 日本口腔科学会雑誌, 73(2) (2), 113 - 113, Japanese


• Transcutaneous carbon dioxide suppresses skeletal muscle atrophy in a mouse model of oral squamous cell carcinoma.

  Aki Sasaki, Daisuke Takeda, Hotaka Kawai, Yoshiaki Tadokoro, Aki Murakami, Nanae Yatagai, Satomi Arimoto, Hitoshi Nagatsuka, Masaya Akashi, Takumi Hasegawa

  Cancer cachexia causes skeletal muscle atrophy, impacting the treatment and prognosis of patients with advanced cancer, but no treatment has yet been established to control cancer cachexia. We demonstrated that transcutaneous application of carbon dioxide (CO2) could improve local blood flow and reduce skeletal muscle atrophy in a fracture model. However, the effects of transcutaneous application of CO2 in cancer-bearing conditions are not yet known. In this study, we calculated fat-free body mass (FFM), defined as the skeletal muscle mass, and evaluated the expression of muscle atrophy markers and uncoupling protein markers as well as the cross-sectional area (CSA) to investigate whether transcutaneous application of CO2 to skeletal muscle could suppress skeletal muscle atrophy in cancer-bearing mice. Human oral squamous cell carcinoma was transplanted subcutaneously into the upper dorsal region of nude mice, and 1 week later, CO2 gas was applied to the legs twice a week for 4 weeks and FFM was calculated by bioimpedance spectroscopy. After the experiment concluded, the quadriceps were extracted, and muscle atrophy markers (muscle atrophy F-box protein (MAFbx), muscle RING-finger protein 1 (MuRF-1)) and uncoupling protein markers (uncoupling protein 2 (UCP2) and uncoupling protein 3 (UCP3)) were evaluated by real-time polymerase chain reaction and immunohistochemical staining, and CSA by hematoxylin and eosin staining. The CO2-treated group exhibited significant mRNA and protein expression inhibition of the four markers. Furthermore, immunohistochemical staining showed decreased MAFbx, MuRF-1, UCP2, and UCP3 in the CO2-treated group. In fact, the CSA in hematoxylin and eosin staining and the FFM revealed significant suppression of skeletal muscle atrophy in the CO2-treated group. We suggest that transcutaneous application of CO2 to skeletal muscle suppresses skeletal muscle atrophy in a mouse model of oral squamous cell carcinoma.

  2024, PloS one, 19(4) (4), e0302194, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Risk factors associated with prognosis of patients with medication-related osteonecrosis of the jaw.

  Yoshiaki Tadokoro, Takumi Hasegawa, Daisuke Takeda, Aki Murakami, Nanae Yatagai, Satomi Arimoro, Eiji Iwata, Izumi Saito, Junya Kusumoto, Masaya Akashi

  OBJECTIVES: Medication-related osteonecrosis of the jaw (MRONJ) is a severe adverse effect of antiresorptive and/or antiangiogenic agents. As the treatment application for MRONJ is controversial, we aimed to identify the risk factors for poor prognosis and to help determine appropriate management. METHODS: This study included 119 patients. Relevant clinical data were obtained for all the patients. In computed tomography images, osteosclerosis, osteolysis, cortical perforation (buccal or lingual), periosteal reaction, and sequestration were evaluated. RESULTS: Multivariate analyses showed statistically significant associations between poor prognosis in patients with MRONJ and conservative treatment alone (hazard ratio [HR] 1.89), osteolysis (HR 4.67), and the absence of sequestration (HR 5.33). CONCLUSIONS: Conservative treatment alone without clear objectives needs to be avoided, and osteolytic change could be the criteria for surgical intervention. As the boundary between the lesion and vital bone is indistinct, we recommend extensive surgery in cases with unpredictable sequestration.

  Nov. 2023, Head & neck, English, International magazine

  Scientific journal


• The Efficacy of Carbon Dioxide Paste in Alleviating Pain in Patients After Neck Dissection: Protocol for a Double-Blinded, Randomized Controlled Trial.

  Yoshiaki Tadokoro, Daisuke Takeda, Izumi Saito, Nanae Yatagai, Yasumasa Kakei, Masaya Akashi, Takumi Hasegawa

  BACKGROUND: Head and neck cancers that cause severe aesthetic and functional disorders normally metastasize to the cervical lymph nodes. Patients with cervical lymph node metastasis are undergoing neck dissection. Shoulder complaints are common after neck dissection, with patients reporting symptoms such as pain, weakness, shoulder droop, and disability. However, no safe and effective treatment is available for this condition at present. We will conduct a double-blinded, randomized controlled trial to evaluate the efficacy of carbon dioxide (CO2) paste in relieving pain in patients after neck dissection. OBJECTIVE: This will be the first clinical study to compare the efficacy of CO2 paste with placebo in relieving postoperative pain in patients who underwent neck dissection. METHODS: We will perform this trial at the Kobe University Hospital in Japan. Patients will be randomized 1:1 into the CO2 paste and control groups. Patients in the CO2 paste group will have the CO2 paste applied to the cervical surface skin for 10 minutes once per day for 14 consecutive days. The primary end point of the study is a change in the visual analog scale (VAS) scores of neck pain from baseline on day 1 (preapplication) to the end of drug application (day 15). Secondary end points include changes in the following parameters from baseline on day 1 to the end of drug application (day 15) or the study (day 29): neck pain VAS score (days 1-29), grip strength (days 1-15 and 1-29), VAS scores for subjective symptoms (the feeling of strangulation, numbness, swelling, and warmth in the neck and shoulder region) for days 1-15 and 1-29, whether the VAS score improved more than 30% (days 1-15), the arm abduction test (days 1-15 and 1-29), shoulder range of motion (abduction and flexion) for days 1-15 and 1-29, occurrence of skin disorders, and occurrence of serious side effects. Periodic monitoring will be conducted for participants during the trial. This study was approved by the certified review board of Kobe University. RESULTS: The intervention commenced in May 2021 and will continue until March 2024. The collected data will provide information on the efficacy of the CO2 paste treatment. The primary end point will be compared using the Wilcoxon test, with the 1-sided significance level set at 5%. Each evaluation item will be summarized. Secondary efficacy end points will be analyzed to provide additional insights into the primary analysis. Findings based on the treatment effects are expected to be submitted for publication in 2025. CONCLUSIONS: This trial will provide exploratory evidence of the efficacy and safety of CO2 paste in relieving pain in patients after neck dissection. TRIAL REGISTRATION: Japan Registry of Clinical Trials (jRCT) identifier: jRCTs051210028; https://jrct.niph.go.jp/en-latest-detail/jRCTs051210028. INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID): DERR1-10.2196/50500.

  Nov. 2023, JMIR research protocols, 12, e50500, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Development of Cu application using dual-energy computed tomography for detecting medication-related osteonecrosis of the jaw.

  Kazuki Shigenaga, Satomi Arimoto, Masahiro Kubo, Takumi Sato, Yujiro Hiraoka, Daisuke Takeda, Takumi Hasegawa, Kiyosumi Kagawa, Masaya Akashi

  INTRODUCTION: The present study developed an application using dual-energy computed tomography (DECT) focused on Cu for detecting medication-related osteonecrosis of the jaw (MRONJ). MATERIALS AND METHODS: First, we performed two types of phantom studies using a Cu wire syringe and pig mandible with Cu wire to detect Cu on DECT. Second, DECT examinations of 44 patients with MRONJ were performed to compare lesion and normal bone sites using single-energy CT, DECT-virtual non-calcium (VNCa), and DECT-Cu applications. Quantitative analyses of VNCa CT and CT values were performed, and a cut-off value was calculated using receiver operating characteristic analysis. Third, we compared the Cu content in the MRONJ and normal bone groups using inductively coupled plasma atomic emission spectroscopy (ICP-AES). RESULTS: The material-specific differences in attenuation between the two different energies enabled the accurate separation of Cu from Ca in phantom studies. The sensitivity and specificity for single-energy CT, DECT-VNCa, and DECT-Cu applications were 97.7% and 2.3%, 86.4% and 81.8%, and 88.6% and 97.7%, respectively. Thus, VNCa CT values obtained on DECT-Cu application images showed the highest area under the curve value and maximal diagnostic efficacy in differentiating lesion sites from normal bone sites. On ICP-AES analyses, the Cu content was significantly higher in the MRONJ group than in the normal bone group. CONCLUSION: DECT-Cu application demonstrated better diagnostic performance in detecting MRONJ compared with single-energy CT or DECT-VNCa.

  Nov. 2023, Journal of bone and mineral metabolism, 41(6) (6), 865 - 876, English, Domestic magazine

  Scientific journal


• 当科における口腔癌癌患者の下顎の広範囲顎骨支持型装置に際し口腔前庭拡張術を要した11例

  高木 ひかる, 筧 康正, 白井 達也, 平岡 佑二郎, 有本 智美, 武田 大介, 木本 明, 長谷川 巧実, 明石 昌也

  (公社)日本顎顔面インプラント学会, Nov. 2023, Japanese Journal of Maxillo Facial Implants, 22(3) (3), 242 - 242, Japanese


• Photodynamic therapy with verteporfin accelerates apoptotic bleb formation in human ameloblastoma.

  Junya Yamashita, Akira Kimoto, Shun Teraoka, Yujiro Hiraoka, Daisuke Takeda, Yasumasa Kakei, Manabu Shigeoka, Takumi Hasegawa, Masaya Akashi

  OBJECTIVE: Although benign, ameloblastoma is a locally aggressive lesion in some patients and the development of additional treatments is needed. Verteporfin (VP) is a photosensitizer exhibiting considerable photocytotoxicity in various tumor cells. We aimed to investigate the effects of verteporfin photodynamic therapy (VP PDT) on ameloblastoma. METHODS: Eighteen patients who underwent surgery for ameloblastoma were randomly selected. We performed an immunohistochemical assessment to investigate the expression of low-density lipoprotein receptor (LDLR) and Yes-associated protein (YAP), targets of VP, in human ameloblastoma tissues and cultured human ameloblastoma cell line (HAM1). The effect of VP PDT on cell proliferation and apoptosis in HAM1 was analyzed. RESULTS: The expression of LDLR and YAP were detected in human ameloblastoma tissues and HAM1. LDLR expression was significantly higher in patients who had previously undergone surgery than in patients who were receiving it for the first time. The cytotoxic effect of the combination of low-concentration VP administration and laser irradiation was comparable to high-concentration VP administration with and without laser irradiation. The addition of laser irradiation to VP administration significantly accelerated apoptotic bleb formation compared with VP administration alone. CONCLUSION: VP PDT has the potential to become an additional treatment for large-sized ameloblastoma.

  Oct. 2023, Oral diseases, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effect of Frequent Computed Tomography Examinations with Contrast Media on the Renal Function of Patients with Oral Squamous Cell Cancer and an Evaluation of Risk Factors for Post-Operative Chronic Kidney Disease

  Takumi Hasegawa, Aya Matsuda, Rika Amano, Izumi Saito, Daisuke Takeda, Yasumasa Kakei, Akira Kimoto, Akiko Sakakibara, Masaya Akashi

  Sep. 2023, JOURNAL OF MAXILLOFACIAL & ORAL SURGERY, English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Relationship of Mitochondrial-Related Protein Expression with the Differentiation, Metastasis, and Poor Prognosis of Oral Squamous Cell Carcinoma.

  Aki Murakami, Daisuke Takeda, Junya Hirota, Izumi Saito, Rika Amano-Iga, Nanae Yatagai, Satomi Arimoto, Yasumasa Kakei, Masaya Akashi, Takumi Hasegawa

  Aug. 2023, Cancers, 15(16) (16), English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Author Correction: Risk factors for pathological fracture in patients with mandibular osteoradionecrosis.

  Hiroaki Ohori, Eiji Iwata, Daisuke Takeda, Junya Kusumoto, Takumi Hasegawa, Masaya Akashi

  Jul. 2023, Scientific reports, 13(1) (1), 11216 - 11216, English, International magazine


• 経皮的炭酸ガスペーストによるラット筋損傷モデルにおける瘢痕化防止と筋再生促進効果

  廣田 純也, 長谷川 巧実, 伊賀 利香, 八谷 奈苗, 黒澤 泉, 武田 大介, 筧 康正, 木本 明, 明石 昌也

  (NPO)日本口腔科学会, Jul. 2023, 日本口腔科学会雑誌, 72(2) (2), 166 - 166, Japanese


• Incidence of antiresorptive agent-related osteonecrosis of the jaw: A multicenter retrospective epidemiological study in Hyogo Prefecture, Japan.

  Masanori Nashi, Hiromitsu Kishimoto, Masaki Kobayashi, Akira Tachibana, Motoo Suematsu, Shigeyoshi Fujiwara, Yoshiyuki Ota, Susumu Hashitani, Takeshi Shibatsuji, Tetsuya Nishida, Kazuma Fujimura, Shungo Furudoi, Yoshiki Ishida, Shoichiro Ishii, Tsuyoshi Fujita, Soichi Iwai, Takashi Shigeta, Takeshi Harada, Daisuke Miyai, Daisuke Takeda, Masaya Akashi, Kazuma Noguchi, Toshihiko Takenobu

  Jul. 2023, Journal of dental sciences, 18(3) (3), 1156 - 1163, English, International magazine

  Scientific journal


• Expression of JCAD and EGFR in Perineurial Cell-Cell Junctions of Human Inferior Alveolar Nerve.

  Yujiro Hiraoka, Megumi Matsumura, Yasumasa Kakei, Daisuke Takeda, Manabu Shigeoka, Akira Kimoto, Takumi Hasegawa, Masaya Akashi

  Jun. 2023, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 71(6) (6), 321 - 332, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Local application of a transcutaneous carbon dioxide paste prevents excessive scarring and promotes muscle regeneration in a bupivacaine-induced rat model of muscle injury.

  Junya Hirota, Takumi Hasegawa, Atsuyuki Inui, Daisuke Takeda, Rika Amano-Iga, Nanae Yatagai, Izumi Saito, Satomi Arimoto, Masaya Akashi

  Apr. 2023, International wound journal, 20(4) (4), 1151 - 1159, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Risk factors for pathological fracture in patients with mandibular osteoradionecrosis.

  Hiroaki Ohori, Eiji Iwata, Daisuke Takeda, Junya Kusumoto, Takumi Hasegawa, Masaya Akashi

  Apr. 2023, Scientific reports, 13(1) (1), 5367 - 5367, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Transcutaneous carbon dioxide application suppresses the expression of cancer-associated fibroblasts markers in oral squamous cell carcinoma xenograft mouse model.

  Yoshiaki Tadokoro, Daisuke Takeda, Aki Murakami, Nanae Yatagai, Izumi Saito, Satomi Arimoto, Yasumasa Kakei, Masaya Akashi, Takumi Hasegawa

  2023, PloS one, 18(8) (8), e0290357, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effect of compression on mandibular fracture haematoma-derived cells.

  Satomi Arimoto, Takumi Hasegawa, Eiji Iwata, Daisuke Takeda, Masaya Akashi

  Nov. 2022, The British journal of oral & maxillofacial surgery, 60(9) (9), 1216 - 1223, English, International magazine

  Scientific journal


• Exploratory clinical trial to evaluate the efficacy and safety of carbon dioxide paste in healthy people.

  Nanae Yatagai, Takumi Hasegawa, Katsusuke Kyotani, Tomohiro Noda, Rika Amano, Izumi Saito, Satomi Arimoto, Daisuke Takeda, Yasumasa Kakei, Masaya Akashi

  Jul. 2022, Medicine, 101(29) (29), e29511, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Factors Associated with Treatment Outcomes and Pathological Features in Patients with Osteoradionecrosis: A Retrospective Study.

  Yoshiaki Tadokoro, Takumi Hasegawa, Daisuke Takeda, Aki Murakami, Nanae Yatagai, Eiji Iwata, Izumi Saito, Junya Kusumoto, Masaya Akashi

  A standard treatment for osteoradionecrosis (ORN) has not yet been established because of the diversity. Therefore, identifying the risk factors for a poor prognosis is essential. This study retrospectively investigated the factors associated with the prognosis of ORN in 68 patients. Relevant clinical data of all patients were obtained. Of the patients, 16 who underwent extensive surgery underwent histopathological analysis. The necrotic changes of the anterior and posterior margins in the cortical and cancellous bones were investigated. Multivariate analyses showed statistically significant associations between poor prognosis in patients with ORN and high radiation dose (hazard ratio [HR] 1.15), orocutaneous fistula (HR 2.93), and absence of sequestration (HR 2.49). Histopathological analysis showed a viable anterior margin of the middle portion of the cortical bone for all recovered cases; in contrast, most cases (75%) with a poor prognosis showed necrotic changes. The anterior margin of the cancellous bone was viable and resilient to high irradiation, regardless of the prognosis. These results suggest that patients with orocutaneous fistula should receive early surgical intervention, even if the affected area is limited or asymptomatic. In extensive surgery, a sufficient safety margin of necrotic bone, particularly in the anterior region, is required to improve the prognosis.

  May 2022, International journal of environmental research and public health, 19(11) (11), English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Success of dental implants in patients with large bone defect and analysis of risk factors for implant failure: a non-randomized retrospective cohort study.

  Takumi Hasegawa, Aki Sasaki, Izumi Saito, Satomi Arimoto, Nanae Yatagai, Yujiro Hiraoka, Daisuke Takeda, Yasumasa Kakei, Masaya Akashi

  Mar. 2022, Clinical oral investigations, 26(3) (3), 2743 - 2750, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Actinomycosis of the Lower Lip: Report of a Case.

  Manabu Shigeoka, Daisuke Takeda, Masaya Akashi

  2022, Case reports in dentistry, 2022, 6121315 - 6121315, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Local Application of Transcutaneous Carbon Dioxide Paste Decreases Inflammation and Accelerates Wound Healing.

  Rika Amano-Iga, Takumi Hasegawa, Daisuke Takeda, Aki Murakami, Nanae Yatagai, Izumi Saito, Satomi Arimoto, Yasumasa Kakei, Akiko Sakakibara, Masaya Akashi

  Nov. 2021, Cureus, 13(11) (11), e19518, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Peripheral facial nerve schwannoma at the inferior mandibular margin: a case report.

  Akiko Sakakibara, Takumi Hasegawa, Daisuke Takeda, Junya Kusumoto, Shunsuke Sakakibara, Masaya Akashi

  Jul. 2021, Journal of surgical case reports, 2021(7) (7), rjab299, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A Case Report of Tongue Lymphoepithelial Carcinoma with a Histological Diagnostic Dilemma.

  Daisuke Takeda, Manabu Shigeoka, Tenyu Sugano, Nanae Yatagai, Takumi Hasegawa, Masaya Akashi

  Jun. 2021, Diagnostics (Basel, Switzerland), 11(6) (6), English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Occurrence and Treatment Outcome of Late Complications After Free Fibula Flap Reconstruction for Mandibular Osteoradionecrosis.

  Junya Yamashita, Masaya Akashi, Daisuke Takeda, Junya Kusumoto, Takumi Hasegawa, Kazunobu Hashikawa

  Mar. 2021, Cureus, 13(3) (3), e13833, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Can CT predict the development of oroantral fistula in patients undergoing maxillary third molar removal?

  Eiji Iwata, Takumi Hasegawa, Masaki Kobayashi, Akira Tachibana, Naoki Takata, Toshiya Oko, Daisuke Takeda, Yoshiki Ishida, Tsuyoshi Fujita, Ikuko Goto, Junichiro Takeuchi, Masaya Akashi

  Mar. 2021, Oral and maxillofacial surgery, 25(1) (1), 7 - 17, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Time to Recurrence Associated With Poor Prognosis in Japanese Oral Squamous Cell Carcinoma Patients

  Takumi Hasegawa, Erina Kobayashi, Rika Amano, Izumi Saito, Daisuke Takeda, Yasumasa Kakei, Akira Kimoto, Akiko Sakakibara, Masaya Akashi

  Feb. 2021, JOURNAL OF MAXILLOFACIAL & ORAL SURGERY, 21(3) (3), 856 - 864, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The prospective evaluation and risk factors of dysphagia after surgery in patients with oral cancer.

  Takumi Hasegawa, Nanae Yatagai, Tatsuya Furukawa, Emi Wakui, Izumi Saito, Daisuke Takeda, Yasumasa Kakei, Akiko Sakakibara, Ken-Ichi Nibu, Masaya Akashi

  Jan. 2021, Journal of otolaryngology - head & neck surgery = Le Journal d'oto-rhino-laryngologie et de chirurgie cervico-faciale, 50(1) (1), 4 - 4, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Bacterial Colonization of the Condyle in Patients with Advanced Mandibular Osteoradionecrosis: Analysis of Hemimandibulectomy Specimens.

  Daisuke Takeda, Kazunobu Hashikawa, Manabu Shigeoka, Maki Kanzawa, Nanae Yatagai, Satomi Arimoto, Junya Kusumoto, Takumi Hasegawa, Hiroto Terashi, Masaya Akashi

  Advanced mandibular osteoradionecrosis (ORN) sometimes requires extended resection (e.g., hemimandibulectomy). Bacterial infection contributes to ORN pathogenesis. To control infection and determine the extent of debridement required, an understanding of bacterial spread within sites of mandibular ORN is important. The current study used a histopathological approach to assess bacterial colonization in the mandibular condyle and elucidate possible paths of bacterial spread towards the mandibular condyle. Four hemimandibulectomy specimens were selected. Areas of bone destruction were macroscopically assessed and confirmed using hematoxylin and eosin staining. Bacterial presence within mandibular condyle was confirmed with Gram staining. Bone exposure was observed in the molar area in all specimens. Macroscopic bone destruction was apparent especially near the medial side of the cortical wall. Gram staining revealed bacterial colonization of the mandibular condyle in three of the four specimens. In conclusion, bacteria tended to spread posteriorly and through the medial side of the mandibular cortical wall. In patients with advanced ORN, the potential for bacterial colonization of the mandibular condyle should be considered during treatment.

  2021, International journal of dentistry, 2021, 9998397 - 9998397, English, International magazine

  Scientific journal


• Transcutaneous Carbon Dioxide Decreases Immunosuppressive Factors in Squamous Cell Carcinoma In Vivo.

  Nanae Yatagai, Takumi Hasegawa, Rika Amano, Izumi Saito, Satomi Arimoto, Daisuke Takeda, Yasumasa Kakei, Masaya Akashi

  2021, BioMed research international, 2021, 5568428 - 5568428, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Which symptoms negatively affect the oral health-related quality of life in patients with osteonecrosis of the jaw?

  Takumi Sato, Junya Kusumoto, Daisuke Takeda, Megumi Kishimoto, Masahiko Kashin, Shungo Furudoi, Masaya Akashi

  Aug. 2020, Oral surgery, oral medicine, oral pathology and oral radiology, 130(2) (2), 175 - 180, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Neutrophil-lymphocyte ratio associated with poor prognosis in oral cancer: a retrospective study.

  Takumi Hasegawa, Tomoya Iga, Daisuke Takeda, Rika Amano, Izumi Saito, Yasumasa Kakei, Junya Kusumoto, Akira Kimoto, Akiko Sakakibara, Masaya Akashi

  Jun. 2020, BMC cancer, 20(1) (1), 568 - 568, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effects of preoperative dental examination and oral hygiene instruction on surgical site infection after hepatectomy: a retrospective study.

  Takumi Hasegawa, Daisuke Takeda, Motofumi Tanaka, Rika Amano, Izumi Saito, Yasumasa Kakei, Akira Kimoto, Takumi Fukumoto, Masaya Akashi

  May 2020, Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer, 29(2) (2), 653 - 659, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effect of low-intensity pulsed ultrasound after intraoral vertical ramus osteotomy.

  Satomi Arimoto, Takumi Hasegawa, Daisuke Takeda, Chizu Tateishi, Masaya Akashi, Shungo Furudoi, Takahide Komori

  Dec. 2019, Oral surgery, oral medicine, oral pathology and oral radiology, 128(6) (6), 581 - 589, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Dental Rehabilitation for Free Fibula Flap-Reconstructed Mandible with Scar Contracture: A Technical Note.

  Masaya Akashi, Kousuke Matsumoto, Daisuke Takeda, Junya Yamashita, Nanae Yatagai, Kazunobu Hashikawa, Takahide Komori

  Jun. 2019, Dentistry journal, 7(3) (3), English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A radiologic evaluation of the incidence and morphology of maxillary sinus septa in Japanese dentate maxillae.

  Daisuke Takeda, Takumi Hasegawa, Izumi Saito, Satomi Arimoto, Masaya Akashi, Takahide Komori

  Jun. 2019, Oral and maxillofacial surgery, 23(2) (2), 233 - 237, English, International magazine

  [Refereed]

  Scientific journal


• Induced Pluripotent Stem Cell-related Genes Correlate With Poor Prognoses of Oral Squamous Cell Carcinoma.

  Yumi Muraki, Takumi Hasegawa, Daisuke Takeda, Takeshi Ueha, Eiji Iwata, Izumi Saito, Rika Amano, Akiko Sakakibara, Masaya Akashi, Takahide Komori

  Mar. 2019, Anticancer research, 39(3) (3), 1205 - 1216, English, International magazine

  [Refereed]

  Scientific journal


• 後天性血友病A患者に対して凝固第VII因子補充療法を行うも重篤な抜歯後口腔咽頭喉頭血腫を生じた1例

  武田 大介, 小松原 秀紀, 古森 孝英

  (公社)日本口腔外科学会, Jan. 2019, 日本口腔外科学会雑誌, 65(1) (1), 10 - 15, Japanese


• A literature review of perioperative antibiotic administration in surgery for medication-related osteonecrosis of the jaw.

  Masaya Akashi, Junya Kusumoto, Daisuke Takeda, Takashi Shigeta, Takumi Hasegawa, Takahide Komori

  Dec. 2018, Oral and maxillofacial surgery, 22(4) (4), 369 - 378, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Effect of local application of transcutaneous carbon dioxide on survival of random-pattern skin flaps.

  Izumi Saito, Takumi Hasegawa, Takeshi Ueha, Daisuke Takeda, Eiji Iwata, Satomi Arimoto, Akiko Sakakibara, Masaya Akashi, Shunsuke Sakakibara, Yoshitada Sakai, Hiroto Terashi, Takahide Komori

  Nov. 2018, Journal of plastic, reconstructive & aesthetic surgery : JPRAS, 71(11) (11), 1644 - 1651, English, International magazine

  [Refereed]

  Scientific journal


• Lymphangiogenesis and Lymph Node Metastasis in Oral Squamous Cell Carcinoma.

  Satomi Arimoto, Takumi Hasegawa, Daisuke Takeda, Izumi Saito, Rika Amano, Masaya Akashi, Takahide Komori

  Nov. 2018, Anticancer research, 38(11) (11), 6157 - 6162, English, International magazine

  [Refereed]

  Scientific journal


• Transcutaneous carbon dioxide enhances the antitumor effect of radiotherapy on oral squamous cell carcinoma.

  Eiji Iwata, Takumi Hasegawa, Takeshi Ueha, Daisuke Takeda, Izumi Saito, Teruya Kawamoto, Toshihiro Akisue, Yoshitada Sakai, Ryohei Sasaki, Ryosuke Kuroda, Takahide Komori

  Jul. 2018, Oncology reports, 40(1) (1), 434 - 442, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Differences between osteoradionecrosis and medication-related osteonecrosis of the jaw.

  Masaya Akashi, Satoshi Wanifuchi, Eiji Iwata, Daisuke Takeda, Junya Kusumoto, Shungo Furudoi, Takahide Komori

  Mar. 2018, Oral and maxillofacial surgery, 22(1) (1), 59 - 63, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A multicenter retrospective study of the risk factors associated with medication-related osteonecrosis of the jaw after tooth extraction in patients receiving oral bisphosphonate therapy: can primary wound closure and a drug holiday really prevent MRONJ?

  T. Hasegawa, A. Kawakita, N. Ueda, R. Funahara, A. Tachibana, M. Kobayashi, E. Kondou, D. Takeda, Y. Kojima, S. Sato, S. Yanamoto, H. Komatsubara, M. Umeda, T. Kirita, H. Kurita, Y. Shibuya, T. Komori, Japanese Study Group of Cooperative Dentistry with Medicine (JCDM)

  Springer London, Aug. 2017, Osteoporosis International, 28(8) (8), 2465 - 2473, English

  [Refereed]

  Scientific journal


• A rare case report of mandibular condyle metastasis of renal pelvic and ureteral cancer

  武田大介, 武田大介, 長谷川巧実, 日向信之, 重岡学, 小林正樹, 小林正樹, 小松原秀紀, 小松原秀紀, 南川勉, 古森孝英

  (一社)日本口腔腫瘍学会, Jun. 2017, 日本口腔腫瘍学会誌, 29(2) (2), 45‐51(J‐STAGE) - 51, Japanese


• Postoperative drainage in head and neck surgery for oral cancer

  Izumi Saito, Takumi Hasegawa, Eiji Iwata, Natsuki Yonezawa, Satomi Arimoto, Daisuke Takeda, Akiko Sakakibara, Masaya Akashi, Tsutomu Minamikawa, Takahide Komori

  May 2017, Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, 29(3) (3), 217 - 221, English

  [Refereed]

  Scientific journal


• Induced Pluripotent-stem-cell Related Genes Contribute to De-differentiation in Oral Squamous Cell Carcinoma.

  Daisuke Takeda, Takumi Hasegawa, Takeshi Ueha, Eiji Iwata, Risa Harada, Akiko Sakakibara, Teruya Kawamoto, Tsutomu Minamikawa, Yoshitada Sakai, Takahide Komori

  Mar. 2017, Anticancer research, 37(3) (3), 1075 - 1082, English, International magazine

  [Refereed]

  Scientific journal


• Survival of Brånemark System Mk III implants and analysis of risk factors associated with implant failure

  T. Hasegawa, S. Kawabata, D. Takeda, E. Iwata, I. Saito, S. Arimoto, A. Kimoto, M. Akashi, H. Suzuki, T. Komori

  Churchill Livingstone, Feb. 2017, International Journal of Oral and Maxillofacial Surgery, 46(2) (2), 267 - 273, English

  [Refereed]

  Scientific journal


• A Case of an Aberrant Tongue Piercing Needle above the Floor of the Mouth Removed in General Anesthesia

  KOMATSUBARA HIDEKI, TAKEDA DAISUKE, FUJIBAYASHI JUNKO, KOMORI TAKAHIDE

  The Japanese Society of Oral Diagnosis / Oral Medicine, 2017, Japanese Journal of Oral Diagnosis / Oral Medicine, 30(1) (1), 18 - 22, Japanese


• Prognosis of oral squamous cell carcinoma patients with level IV/V metastasis: An observational study.

  Takumi Hasegawa, Yasuyuki Shibuya, Daisuke Takeda, Eiji Iwata, Izumi Saito, Yasumasa Kakei, Akiko Sakakibara, Masaya Akashi, Tsutomu Minamikawa, Takahide Komori

  Jan. 2017, Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery, 45(1) (1), 145 - 149, English, International magazine

  [Refereed]

  Scientific journal


• Upregulated Expression of Transient Receptor Potential Cation Channel Subfamily V Receptors in Mucosae of Patients with Oral Squamous Cell Carcinoma and Patients with a History of Alcohol Consumption or Smoking.

  Akiko Sakakibara, Shunsuke Sakakibara, Junya Kusumoto, Daisuke Takeda, Takumi Hasegawa, Masaya Akashi, Tsutomu Minamikawa, Kazunobu Hashikawa, Hiroto Terashi, Takahide Komori

  2017, PloS one, 12(1) (1), e0169723, English, International magazine

  [Refereed]

  Scientific journal


• Risk factors associated with oroantral perforation during surgical removal of maxillary third molar teeth.

  Takumi Hasegawa, Akira Tachibana, Daisuke Takeda, Eiji Iwata, Satomi Arimoto, Akiko Sakakibara, Masaya Akashi, Takahide Komori

  Dec. 2016, Oral and maxillofacial surgery, 20(4) (4), 369 - 375, English, International magazine

  [Refereed]

  Scientific journal


• Decreased mitochondrial copy numbers in oral squamous cell carcinoma.

  Daisuke Takeda, Takumi Hasegawa, Takeshi Ueha, Akiko Sakakibara, Teruya Kawamoto, Tsutomu Minamikawa, Yoshitada Sakai, Takahide Komori

  Aug. 2016, Head & neck, 38(8) (8), 1170 - 5, English, International magazine

  [Refereed]

  Scientific journal


• Transcutaneous carbon dioxide suppresses epithelial-mesenchymal transition in oral squamous cell carcinoma.

  Eiji Iwata, Takumi Hasegawa, Daisuke Takeda, Takeshi Ueha, Teruya Kawamoto, Toshihiro Akisue, Yoshitada Sakai, Takahide Komori

  Apr. 2016, International journal of oncology, 48(4) (4), 1493 - 8, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Evaluation and comparison of CT values in bisphosphonate-related osteonecrosis of the jaw

  Yusuke Imai, Takumi Hasegawa, Daisuke Takeda, Junya Kusumoto, Masaya Akashi, Shinshou Ri, Shungo Furudoi, Takahide Komori

  Jan. 2016, Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, 28(1) (1), 19 - 25, English

  [Refereed]

  Scientific journal


• Risk factors associated with postoperative delirium after surgery for oral cancer.

  Takumi Hasegawa, Izumi Saito, Daisuke Takeda, Eiji Iwata, Natsuki Yonezawa, Yasumasa Kakei, Akiko Sakakibara, Masaya Akashi, Tsutomu Minamikawa, Takahide Komori

  Sep. 2015, Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery, 43(7) (7), 1094 - 8, English, International magazine

  [Refereed]

  Scientific journal


• Low-intensity pulsed ultrasound enhances bone morphogenetic protein expression of human mandibular fracture haematoma-derived cells

  W. Huang, T. Hasegawa, Y. Imai, D. Takeda, M. Akashi, T. Komori

  Churchill Livingstone, Jul. 2015, International Journal of Oral and Maxillofacial Surgery, 44(7) (7), 929 - 935, English

  [Refereed]

  Scientific journal


• Risk factors associated with distant metastasis in patients with oral squamous cell carcinoma.

  Takumi Hasegawa, Makiko Tanakura, Daisuke Takeda, Akiko Sakakibara, Masaya Akashi, Tsutomu Minamikawa, Takahide Komori

  Jun. 2015, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery, 152(6) (6), 1053 - 60, English, International magazine

  [Refereed]

  Scientific journal


• The osteogenic activity of human mandibular fracture haematoma-derived progenitor cells is affected by bisphosphonate in vitro

  Y. Imai, T. Hasegawa, D. Takeda, M. Akashi, T. Komori

  Churchill Livingstone, Mar. 2015, International Journal of Oral and Maxillofacial Surgery, 44(3) (3), 412 - 416, English

  [Refereed]

  Scientific journal


• The osteogenic activity of human mandibular fracture haematoma-derived cells is stimulated by low-intensity pulsed ultrasound in vitro

  Y. Imai, T. Hasegawa, D. Takeda, M. Akashi, S. Y. Lee, T. Niikura, Y. Shibuya, M. Kurosaka, T. Komori

  Mar. 2014, International Journal of Oral and Maxillofacial Surgery, 43(3) (3), 367 - 372, English

  [Refereed]

  Scientific journal


• Transcutaneous carbon dioxide induces mitochondrial apoptosis and suppresses metastasis of oral squamous cell carcinoma in vivo.

  Daisuke Takeda, Takumi Hasegawa, Takeshi Ueha, Yusuke Imai, Akiko Sakakibara, Masaya Minoda, Teruya Kawamoto, Tsutomu Minamikawa, Yasuyuki Shibuya, Toshihiro Akisue, Yoshitada Sakai, Masahiro Kurosaka, Takahide Komori

  2014, PloS one, 9(7) (7), e100530, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Retrospective study of ameloblastoma: the possibility of conservative treatment.

  Takumi Hasegawa, Yusuke Imai, Daisuke Takeda, Daisuke Yasuoka, Shinshou Ri, Takashi Shigeta, Tsutomu Minamikawa, Yasuyuki Shibuya, Takahide Komori

  At our institutions, most cases of the solid or multicystic type were treated as conservatively as possible in order to avoid disadvantages of radical treatment. The aim of present study was to retrospectively analyze the ameloblastoma cases diagnosed at our two institutions, to classify them according to the criteria of the 2005 WHO classification, and to evaluate the possibility of using a conservative approach for the surgical treatment of ameloblastoma. Maxillary cases, unicystic cases, peripheral cases and resection-treated cases were excluded from this study. In 23 tumors of mandibular solid or multicystic ameloblastoma, a patient's age, gender, location, clinical signs, duration, radiographic appearance, preoperative diagnosis, ameloblastoma subtypes, treatment, and recurrence were investigated. The recurrence rate (48.7%) in this study was lower than the reported recurrence rate after conservative treatment for solid or multicystic ameloblastoma and was higher than the reported recurrence rate of ameloblastoma, inclusive of other types. However, all patients who were diagnosed with recurrences have maintained their quality of life and were satisfied for at least several years after the conservative treatment. In conclusion, we demonstrated one possibility that a conservative approach might be employed in the surgical treatment of ameloblastoma (even of the solid or multicystic type).

  Nov. 2013, The Kobe journal of medical sciences, 59(4) (4), E112-21, English, Domestic magazine

  [Refereed]

  Scientific journal

• 当科で新型コロナウイルス感染症に対してPCR検査を行った患者についての考察

  利川 誠将, 武田 大介, 長谷川 巧実, 明石 昌也

  (一社)日本口腔感染症学会, May 2023, 日本口腔感染症学会雑誌, 30(1) (1), 65 - 66, Japanese


• 歯科衛生士が口腔衛生管理を行った患者に対するCOVID-19発症状況について

  岡崎 葉菜, 武田 大介, 片山 めぐみ, 西井 美佳, 村木 友美, 長谷川 巧実, 明石 昌也

  (一社)日本口腔ケア学会, Apr. 2023, 日本口腔ケア学会雑誌, 17(3) (3), 172 - 172, Japanese


• COVID-19感染が契機と考えられたスティーブンスジョンソン症候群患者への口腔衛生管理の1例

  片山 めぐみ, 村木 友美, 岡崎 葉菜, 西井 美佳, 武田 大介, 長谷川 巧実, 明石 昌也

  (一社)日本口腔ケア学会, Apr. 2023, 日本口腔ケア学会雑誌, 17(3) (3), 229 - 229, Japanese


• Efficacy of systemic chemotherapy for patients with oral cancer who are not candidates for local treatment.

  鈴木大貴, 鈴木大貴, 佐藤春樹, 加藤崇雄, 菅省吾, 武田大介, 鵜澤成一, 栗田浩

  2023, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 41st


• Chemoradiotherapy versus radiation therapy alone for unresectable local advanced oral squamous cell carcinoma.

  佐藤春樹, 伊川裕明, 高山香名子, 武田大介, 鈴木大貴, 鈴木大貴, 鵜澤成一, 栗田浩

  2023, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 41st


• Oral and maxillofacial exfoliative cytology based on LBC/Oral Bethesda system: Roles of cytology at hospital dentistry.

  佐々木亜紀, 重岡学, 重岡学, 米田亜紀子, 米田亜紀子, 武田大介, 明石昌也

  2023, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 41st


• Report of the Working Group on surgery (resection)

  柳本惣市, 山川延宏, 山田慎一, 長谷川巧実, 武田大介, 鈴木崇嗣, 松山たまも, 大林史誠, 山崎佐知子, 福谷多恵子, 青木隆幸

  2023, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 41st


• Efficacy of neoadjuvant chemotherapy in resectable oral squamous cell carcinomas.

  釜本宗史, 北村直也, 生駒丈晴, 武田大介, 鈴木大貴, 鈴木大貴, 鵜澤成一, 栗田浩

  2023, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 41st


• Effectiveness of Immunostimulant Nutritional Supplements in Adult Patients with Oral Cancer

  平岡慎一郎, 安部貴大, 渡辺昌広, 武田大介, 鵜澤成一, 栗田浩

  2023, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 41st


• Management of the neck in cN1 cases of oral squamous cell carcinoma

  上田順宏, 武田大介, 比地岡浩志, 鵜澤成一, 栗田浩, 中山洋平, 中村泰士, 柳生貴裕, 山川延宏, 桐田忠昭

  2023, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 41st


• Effect of reconstruction with soft tissue versus obturator after matxillectomy.

  鈴木大貴, 鈴木大貴, 莇生田整治, 金山宏幸, 武田大介, 鵜澤成一, 栗田浩

  2023, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 41st


• Role of induction chemotherapy for locally advanced oral squamous cell carcinoma

  夫才成, 佐藤春樹, 後藤満雄, 丹野咲貴, 武田大介, 鈴木大貴, 鈴木大貴, 鵜澤成一, 栗田浩

  2023, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 41st


• Tube feeding in patients with head and neck cancer undergoing chemoradio-/radio therapy.

  鈴木大貴, 鈴木大貴, 柳本惣市, 齋藤寛一, 齋藤寛一, 武田大介, 鵜澤成一, 栗田浩

  2023, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 41st


• Intra-arterial chemoradiotherapy versus surgery for local advanced oral squamous cell carcinoma.

  佐藤春樹, 高山香名子, 小栗千里, 齋藤寛一, 鈴木大貴, 鈴木大貴, 武田大介, 鵜澤成一, 栗田浩

  2023, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 41st


• Retrospective study on risk factors associated with sustainable success of implant in bone anchored device for wide edentulous area of patients with oral cancer introduced in our department

  早瀬悠夏, 長谷川巧実, 白井達也, 平岡佑二郎, 有本智美, 武田大介, 筧康正, 木本明, 明石昌也

  (公社)日本顎顔面インプラント学会, Nov. 2022, Japanese Journal of Maxillo Facial Implants, 21(3) (3), 237 - 237, Japanese


• 当科における下顎骨びまん性硬化性骨髄炎症例に関する臨床的検討

  早瀬 悠夏, 楠元 順哉, 古土井 春吾, 武田 大介, 明石 昌也

  (NPO)日本口腔科学会, Jul. 2022, 日本口腔科学会雑誌, 71(2) (2), 81 - 81, Japanese


• 放射線性顎骨壊死患者を対象とした予後とリスク因子に関する後ろ向き観察研究

  田所 慶誠, 長谷川 巧実, 佐藤 匠, 平岡 佑二郎, 有本 智美, 武田 大介, 楠元 順哉, 明石 昌也

  (NPO)日本口腔科学会, Jul. 2022, 日本口腔科学会雑誌, 71(2) (2), 137 - 137, Japanese


• 局所的炭酸ガス投与による口腔扁平上皮癌の腫瘍免疫抑制に対する改善効果

  八谷 奈苗, 長谷川 巧実, 武田 大介, 筧 康正, 榊原 晶子, 明石 昌也

  (NPO)日本口腔科学会, Jul. 2022, 日本口腔科学会雑誌, 71(2) (2), 110 - 110, Japanese


• Factors Associated with Treatment Outcomes and Pathological Features in Patients with Osteoradionecrosis: A Retrospective Study.

  Yoshiaki Tadokoro, Takumi Hasegawa, Daisuke Takeda, Aki Murakami, Nanae Yatagai, Eiji Iwata, Izumi Saito, Junya Kusumoto, Masaya Akashi

  27 May 2022, International journal of environmental research and public health, 19(11) (11), English, International magazine


• 口腔粘膜炎に対するエピシル口腔溶液の効果や有効性について

  岡崎 葉菜, 武田 大介, 西井 美佳, 村川 智美, 片山 めぐみ, 澤田 麻衣子, 楠元 順哉, 明石 昌也

  (一社)日本口腔ケア学会, Apr. 2022, 日本口腔ケア学会雑誌, 16(3) (3), 140 - 140, Japanese


• 頭頸部がん放射線療法における口腔カンジダ症に対するミコナゾール貼付薬の有効性について

  西井 美佳, 楠元 順哉, 村川 智美, 岡崎 葉菜, 片山 めぐみ, 澤田 麻衣子, 武田 大介, 明石 昌也

  (一社)日本口腔ケア学会, Apr. 2022, 日本口腔ケア学会雑誌, 16(3) (3), 141 - 141, Japanese


• 半教師あり対照学習による顎骨骨髄炎発症範囲の自動推定

  星野秀晃, 盛田健人, 武田大介, 長谷川巧実, 若林哲史

  2022, 日本生体医工学会大会プログラム・抄録集(Web), 61st


• A case of vestibuloplasty with split thickness skin graft was harvested from reconstructive soft tissue after oral cavity cancer resection

  重永一輝, 有本智美, 武田大介, 木本明, 長谷川巧実, 明石昌也

  (公社)日本顎顔面インプラント学会, Nov. 2021, Japanese Journal of Maxillo Facial Implants, 20(3) (3), 189 - 189, Japanese


• Pre-operative Head CT and Pathological Image Registration using Normalized Cross-Correlation and Dice Coefficient

  松岡志宜, 盛田健人, 武田大介, 長谷川巧実, 若林哲史

  2021, 電子情報通信学会技術研究報告(Web), 120(431(MI2020 47-97)) (431(MI2020 47-97))


• Automatic estimation of the extent of osteomyelitis of the jaw by anomaly detection

  星野秀晃, 盛田健人, 武田大介, 長谷川巧実, 若林哲史

  2021, 電子情報通信学会技術研究報告(Web), 120(431(MI2020 47-97)) (431(MI2020 47-97))


• Effect of percutaneous administration of carbon dioxide to improve tumor immunosuppression

  八谷奈苗, 長谷川巧実, 武田大介, 齊藤泉, 有本智美, 筧康正, 榊原晶子, 明石昌也

  2021, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 39th


• Consideration of non-survival cases in patients with oral squamous cell carcinoma and Future perspectives in a tertiary referral center

  長谷川巧実, 武田大介, 筧康正, 明石昌也

  2021, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 39th


• Automatic image registration of head CT and resected jaw bone CT for the Computer-aided diagnosis of osteoradionecrosis of the jaws

  松岡志宜, 盛田健人, 武田大介, 長谷川巧実, 若林哲史

  (一社)日本医用画像工学会, Sep. 2020, 日本医用画像工学会大会予稿集(CD-ROM), 39回, 30 - 30, Japanese


• 顎骨骨髄炎発症範囲推定のための頭部CT・切除顎骨CTの位置合わせ自動化

  松岡 志宜, 盛田 健人, 武田 大介, 長谷川 巧実, 若林 哲史

  (一社)日本医用画像工学会, Sep. 2020, 日本医用画像工学会大会予稿集, 39回, 173 - 177, Japanese


• 口腔癌cN(+)における根治的頸部郭清範囲の検討

  手島 直則, 窪田 雄一, 入谷 啓介, 蓼原 瞬, 古川 竜也, 四宮 弘隆, 大月 直樹, 武田 大介, 筧 康正, 長谷川 巧実

  (一社)日本頭頸部癌学会, Jul. 2020, 頭頸部癌, 46(2) (2), 183 - 183, Japanese


• 顎骨壊死を契機として切開排膿を要した咀嚼筋隙膿瘍の検討

  下間 祐輝, 武田 大介, 佐々木 亜紀, 小林 英里奈, 八橋 明子, 齋藤 泉, 楠元 順哉, 長谷川 巧実, 古土井 春吾, 明石 昌也

  (NPO)日本口腔科学会, Jul. 2020, 日本口腔科学会雑誌, 69(2) (2), 121 - 121, Japanese


• 当科で歯科介入を行った高用量骨吸収抑制薬使用患者におけるMRONJ発症とリスク因子についての検討

  廣田 純也, 長谷川 巧実, 武田 大介, 筧 康正, 楠元 順哉, 榊原 晶子, 木本 明, 明石 昌也

  (NPO)日本口腔科学会, Jul. 2020, 日本口腔科学会雑誌, 69(2) (2), 153 - 153, Japanese


• アルツハイマー型認知症患者の口腔内に生じた孤立性形質細胞腫に対して放射線治療が奏功した1例

  棚倉万紀子, 武田大介, 三谷泉, 明石昌也

  2020, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 38th


• 炭酸ガスを使用した効率的な低酸素環境改善による口腔癌の新規治療法の開発と治療抵抗性の改善効果

  長谷川巧実, 武田大介, 齋藤泉, 岩田英治, 筧康正, 榊原晶子, 明石昌也, 南川勉, 古森孝英

  2019, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 37th, 120, Japanese


• 口腔癌におけるPET‐MRI~第二報~

  瓜生開人, 南川勉, 大橋雄高, 大橋雄高, 松田彩, 武田大介, 筧康正, 松井太輝, 榊原晶子, 長谷川巧実, 明石昌也, 古森孝英

  2019, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 37th, 156, Japanese


• 当科におけるインプラント埋入症例と喪失リスク因子に関する後ろ向き観察研究

  長谷川 巧実, 武田 大介, 齋藤 泉, 有本 智美, 筧 康正, 明石 昌也, 鈴木 泰明, 古森 孝英

  (公社)日本顎顔面インプラント学会, Nov. 2018, Japanese Journal of Maxillo Facial Implants, 17(3) (3), 224 - 224, Japanese


• 当科におけるインプラント埋入症例と喪失リスク因子に関する後ろ向き観察研究

  長谷川巧実, 武田大介, 齋藤泉, 有本智美, 筧康正, 明石昌也, 鈴木泰明, 古森孝英

  (公社)日本顎顔面インプラント学会, Nov. 2018, Japanese Journal of Maxillo Facial Implants, 17(3) (3), 224 - 224, Japanese


• 下顎智歯抜歯後の術後出血に関するリスク因子についての検討

  廣田 純也, 長谷川 巧実, 西條 翔, 大山 剛平, 武田 大介, 明石 昌也, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2018, 日本口腔科学会雑誌, 67(2) (2), 131 - 132, Japanese


• 頸部郭清を伴う口腔癌術後合併症に関する臨床的検討

  八谷 奈苗, 長谷川 巧実, 武田 大介, 筧 康正, 松井 太輝, 明石 昌也, 南川 勉, 古土井 春吾, 古森 孝英

  (NPO)日本口腔科学会, Jul. 2018, 日本口腔科学会雑誌, 67(2) (2), 117 - 117, Japanese


• 下顎智歯抜歯後の術後出血に関するリスク因子についての検討

  廣田純也, 長谷川巧実, 西條翔, 大山剛平, 武田大介, 明石昌也, 古森孝英

  2018, 日本口腔科学会雑誌(Web), 67(2) (2)


• 頸部郭清を伴う口腔癌術後合併症に関する臨床的検討

  八谷奈苗, 長谷川巧実, 武田大介, 筧康正, 松井太輝, 明石昌也, 南川勉, 古土井春吾, 古森孝英

  2018, 日本口腔科学会雑誌(Web), 67(2) (2), 117(J‐STAGE), Japanese


• 放射線性下顎骨壊死の診断におけるfollow up PET‐CTの有用性

  鰐渕聡, 明石昌也, 平岡佑二郎, 武田大介, 筧康正, 松井太輝, 長谷川巧実, 南川勉, 古土井春吾, 古森孝英

  2018, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 36th, 161, Japanese


• 口腔癌再建術後Recipient‐site infectionの定義に関する文献レビュー

  明石昌也, 筧康正, 武田大介, 松井太輝, 榊原晶子, 長谷川巧実, 南川勉, 橋川和信, 古森孝英

  2018, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 36th, 149, Japanese


• 口腔癌放射線治療における新規併用療法の開発

  岩田英治, 長谷川巧実, 武田大介, 斎藤泉, 有本智美, 明石昌也, 南川勉, 古森孝英

  2017, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 35th


• 口腔扁平上皮癌における外的刺激受容体(TRPVチャネル)の発現亢進

  榊原晶子, 榊原俊介, 楠元順哉, 楠元順哉, 武田大介, 長谷川巧実, 明石昌也, 南川勉, 橋川和信, 古森孝英

  2016, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 34th


• 口腔扁平上皮癌患者の遠位レベル(Level4,5)への頸部リンパ節転移が予後に与える影響

  定兼啓倫, 長谷川巧実, 渋谷恭之, 渋谷恭之, 米澤奈津季, 武田大介, 高橋佑輔, 筧康正, 明石昌也, 南川勉, 古森孝英

  2016, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 34th, 221, Japanese


• 局所的炭酸ガス投与による皮弁壊死抑制効果

  齊藤泉, 長谷川巧実, 武田大介, 岩田英治, 有本智美, 筧康正, 高橋佑輔, 榊原晶子, 明石昌也, 南川勉, 古森孝英

  2016, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 34th, 208, Japanese


• ロケーター・磁性バーアタッチメントによるインプラントオーバーデンチャー関連合併症

  有本智美, Hasegawa Takumi, 斉藤泉, 岩田英治, 武田大介, Akashi Masaya, Suzuki Yasuaki, 古土井春吾, Komori Takahide

  (公社)日本顎顔面インプラント学会, Nov. 2015, Japanese Journal of Maxillo Facial Implants, 14(3号) (3号), 261 - 261, Japanese


• 当科における頸部郭清術術後の頸部ドレーンに関する臨床的検討

  齊藤泉, 長谷川巧実, 米澤奈津季, 武田大介, 高橋佑輔, 筧康正, 松井太輝, 榊原晶子, 明石昌也, 南川勉, 古森孝英

  2015, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 33rd, 295, Japanese


• 当科における口腔扁平上皮癌遠隔転移例の臨床的検討とリスク因子について

  棚倉万紀子, 長谷川巧実, 米澤奈津季, 武田大介, 高橋佑輔, 筧康正, 松井太輝, 榊原晶子, 明石昌也, 南川勉, 古森孝英

  2015, 日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 33rd, 301, Japanese


• 当科における広範囲顎骨支持型インプラント補綴の臨床統計的検討

  今井 佑輔, 長谷川 巧実, 川畑 真哉, 武田 大介, 明石 昌也, 鈴木 泰明, 古森 孝英

  (公社)日本顎顔面インプラント学会, Nov. 2014, Japanese Journal of Maxillo Facial Implants, 13(3) (3), 128 - 128, Japanese


• ゾレドロネート反復投与による創傷治癒遅延の予防、治療法に関する研究

  古森 孝英, 長谷川 巧実, 今井 佑輔, 武田 大介, 浅井 雅敏

  ライフサイエンス出版(株), Oct. 2014, Osteoporosis Japan, 22(4) (4), 706 - 709, Japanese

• Selective neck dissection vs observation for clinical cervical node negative in oral squamous cell carcinoma patients.

  武田大介, 上田順宏, 助川信太郎, 鈴木大貴, 鈴木大貴, 鵜澤成一, 栗田浩

  日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 2023


• A study of patients who needed to apply for nursing insurance during hospitalization for oral cancer

  武田大介, 長谷川巧実, 筧康正, 明石昌也

  日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 2022


• A case of occlusal recovery by implant-supported overdenture after vascularized fibula flap reconstruction for osteoradionecrosis of the mandible

  武田大介, 有本智美, 長谷川巧実, 明石昌也

  Japanese Journal of Maxillo Facial Implants, Nov. 2021, Japanese, (公社)日本顎顔面インプラント学会


• A case of tongue cancer histopathologically diagnosed as lymphoepithelial carcinoma

  武田大介, 重岡学, 長谷川真子, 村上明希, 八谷奈苗, 長谷川巧実, 明石昌也

  日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 2021


• 口腔扁平上皮癌における人工多能性幹細胞関連因子の発現について

  武田大介, 長谷川巧実, 江崎友美, 榊原晶子, 明石昌也, 南川勉, 古森孝英

  日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 2019


• 口腔扁平上皮癌臨床検体におけるミトコンドリア量について

  武田大介, 長谷川巧実, 今井佑輔, 榊原晶子, 明石昌也, 南川勉, 古森孝英

  日本口腔腫瘍学会総会・学術大会プログラム・抄録集, 2015


• 入院中にアデノウイルス感染を生じた外傷性歯牙脱臼の1症例

  武田 大介, 安東 大器, 中村 純也, 西田 哲也, 河合 峰雄

  日本歯科麻酔学会雑誌, Sep. 2011, Japanese, (一社)日本歯科麻酔学会

• ミトコンドリアの正常化と癌関連線維芽細胞の再教育を目指した新規口腔癌治療法の開発

  長谷川 巧実, 榊原 晶子, 明石 昌也, 武田 大介

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2022 - 31 Mar. 2026


• がん悪液質に着目した口腔がん新規治療法の検討

  武田 大介

  日本学術振興会, 科学研究費助成事業, 若手研究, 神戸大学, 01 Apr. 2020 - 31 Mar. 2024

  口腔がんの増大は経口摂取を妨げる要因となっており、手術までに時間を要する患者では栄養不良の進行が問題となっている。“がん悪液質”は筋肉量減少を主体に体組成が変化する状態であり、食欲不振や臓器機能不全をもたらすが、関与するサイトカインなどが判明されつつも、その治療法はいまだ明らかになっていない。われわれは、癌細胞のエネルギー代謝の変化や低酸素環境に着目し、低酸素環境改善による新規治療法を報告してきた。また、治療抵抗性に関わる癌幹細胞関連因子に着目し、低酸素環境や分化度との関係を報告してきた。本研究の目的は、これらの新規治療法の標的を“がん悪液質”へ適応し、体組成の変化を改善することにより、宿主の衰弱を防ぐとともに癌細胞へのエネルギー供給を阻止する方法を確立することである。 実験動物用体組成計を購入し、in vitroの検討で、ヒト口腔扁平上皮癌細胞株(HSC-3)を移植したヌードマウスの体組成分析を行った。その結果、非治療群では治療群(局所的炭酸ガス投与群)と比較して、腫瘍移植4週間後の除脂肪量(率)の有意な減少が認められた。


• Development of cancer therapies to improve resistance and reduce side effects

  Hasegawa Takumi

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Kobe University, 01 Apr. 2018 - 31 Mar. 2022

  In the present study, the combination of transcutaneous CO2 and radiation therapy significantly inhibited tumor growth compared with other treatments using a local carbon dioxide gas administration method. In the combination group, anti-human ROS modulator 1 and caspase8 were increased by radiation therapy (RT), and mitochondrial apoptosis (PARP, caspase 3, 9) were increased by transcutaneous CO2 therapy. These results indicated that transcutaneous CO2 could potentially improve the antitumor effect of RT by decreasing the intratumoral hypoxia and increasing the mitochondrial apoptosis. Our findings indicated that CO2 therapy may be a novel adjuvant therapy in combination with RT for OSCC.


• Examination of metastasis suppression effect focusing on tumor microenvironment around cancer stem cells

  Takeda Daisuke

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Kobe University, 01 Apr. 2016 - 31 Mar. 2020

  We investigated the expression of induced pluripotent stem cell related genes in vitro and in clinical samples to identify their relationships with hypoxia and tumorigenesis. Oral squamous cell carcinoma cells were used to show that expression levels of Oct3/4, Sox2, and Nanog were significantly increased in hypoxic condition in vitro and in moderately- and poorly-differentiated samples. The 3-year disease-specific survival rates of patients with high Oct3/4, Sox2 and Klf4 expression levels were significantly decreased. The expression levels of Podoplanin and LYVE-1 were significantly associated with cervical lymph node metastasis.


• Transcutaneous carbon dioxide induces suppresses metastasis of oral squamous cell carcinoma

  HASEGAWA TAKUMI, IWATA Eiji, TAKEDA Daisuke, UEHA Takeshi, SAKAI Yoshitada, KOMORI Takahide

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Kobe University, 01 Apr. 2015 - 31 Mar. 2018

  We previously demonstrated that transcutaneous CO2 suppresses the growth of primary human OSCC and metastasis to the regional lymph nodes by both improving hypoxia and increasing mitochondrial apoptosis in treated tissue. Therefore, we hypothesized that improving hypoxia by transcutaneous CO2 could suppress EMT. In the present study, we investigated whether oxygen conditions affect HIF-1α and EMT factors in OSCC in vivo and whether transcutaneous CO2 affects these factors in OSCC. In the present study, in vivo studies revealed that transcutaneous CO2 increased E-cadherin expression with the decreased expression of HIF-1α, Snail, Slug, N-cadherin, and Vimentin in treatment tumors. These results suggest that transcutaneous CO2 could suppress EMT by improving hypoxia, resulting in the reduction of metastatic potential of OSCC. The findings indicate that transcutaneous CO2 may be able to improve the prognosis of OSCC patients through the suppression of EMT.


• Development of the new treatment in oral cancer related to mitochondrial apoptosis

  HASEGAWA TAKUMI, TAKEDA Daisuke, UEHA Takeshi, KAWAMOTO Teruya, KOMORI Takahide

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Kobe University, 01 Apr. 2012 - 31 Mar. 2015

  In this study, we examined the effects of transcutaneous CO2 on cancer apoptosis and lymphogenous metastasis using human SCC xenografts. Our results showed that transcutaneous CO2 affects expressions of PGC-1α and TFAM and protein levels of cleavage products of caspase-3, caspase-9 and PARP, which relatives mitochondrial apoptosis. They also showed that transcutaneous CO2 significantly inhibits SCC tumor growth and affects expressions of HIF-1α, VEGF, MMP-2 and MMP-9, which play essential roles in tumor angiogenesis, invasion and metastasis. In conclusion, transcutaneous CO2 suppressed tumor growth, increased mitochondrial apoptosis and decreased the number of lymph node metastasis in human SCC by decreasing intra-tumoral hypoxia and suppressing metastatic potential with no observable effect in vivo. Our findings indicate that transcutaneous CO2 could be a novel therapeutic tool for treating human SCC.