Research activity information

• Spontaneous neck hematoma associated with parathyroid adenoma.

  Mitsumasa Okano, Mitsuko Yui, Masanori Teshima, Kazuhiko Sakaguchi

  We should consider parathyroid extraglandular bleeding for patients with acute neck pain and swelling. Evaluation of serum calcium and parathyroid hormone levels is crucial for a suspected neck hematoma associated with parathyroid adenoma.

  Sep. 2024, Clinical case reports, 12(9) (9), e9383, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Amyloid deposition through endocytosis in vascular endothelial cells.

  Seiji Nishikage, Akira Fujisawa, Hiromi Endoh, Hirotaka Sakamoto, Tomohide Suzuki, Maki Kanzawa, Shinichi Ishii, Mitsumasa Okano, Eriko Nitta, Kimikazu Yakushijin, Hidesaku Asakura, Kandai Nozu, Ryo Nitta, Yoshio Katayama, Kazuhiko Sakaguchi

  No mechanistic lead is known for establishing AL amyloid deposits in organs. We here report an electron microscopic (EM) analysis in a case of intestinal AL amyloidosis before initiating treatment for amyloidosis. The dense deposits of amyloid fibrils are concentrated around the small blood vessels in the submucosal area of intestinal tissue. Surprisingly, we observed endothelial cells (ECs) of blood vessels containing plenty of endocytotic (pinocytotic) and transcytotic vesicles at the luminal side and above the basement membrane, indicating the one-way active trafficking of either the immunoglobulin (Ig) light chain or preassembled amyloid fibrils from the luminal side of ECs to the extraluminal area of ECs. Immunoelectron microscopy displayed that the immuno-gold signals were observed in the vascular cavity and the subendothelial area of amyloid deposits. However, there is no sign of an Ig light chain in pinocytotic vesicles. Therefore, the intestinal ECs may actively pump out mainly the preassembled amyloid fibrils (not light chains) from the blood stream into the subendothelial area as a physiologic function.

  Jan. 2024, Experimental hematology, 129, 104129 - 104129, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Glucocorticoid in Combination with a TNF-α Inhibitor: Treatment of Deep Vein Thrombosis in a Patient with Behçet's Disease.

  Mitsumasa Okano, Katsuhiko Yoneda, Yoshihide Ichise, Sentaro Kusuhara, Naokazu Muramae, Kenta Mori, Kazunori Otsui, Kazuhiko Sakaguchi

  A 38-year-old man with deep vein thrombosis associated with Behçet's disease (BD) was admitted to our hospital due to worsening symptoms despite the initiation of direct oral anticoagulants (DOACs). Administration of oral prednisolone and an intravenous anti-tumor necrosis factor-alpha (TNF-α) monoclonal antibody dramatically improved his symptoms. In addition, he was incidentally diagnosed with autosomal dominant polycystic kidney disease, which increases the risk of aortic aneurysms. BD also increases the risk of aortic aneurysms. This case suggests that immunosuppressive treatment is effective in patients with inflammation-related DOAC-refractory venous thrombosis who also suffer from BD.

  Jul. 2023, Internal medicine (Tokyo, Japan), 62(13) (13), 2009 - 2012, English, Domestic magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Distinct hypoglycemic effect of different formulations of a fixed ratio of basal insulin plus glucagon-like peptide-1 receptor agonist in a patient with pancreatic diabetes.

  Tomoko Yamada, Shun-Ichiro Asahara, Maki Kimura-Koyanagi, Yoshikazu Tamori, Naokazu Muramae, Kenta Mori, Mitsumasa Okano, Kazunori Otsui, Kazuhiko Sakaguchi

  UNLABELLED: Fixed-ratio combination injection therapy (FRC) is a fixed-ratio mixture containing basal insulin and glucagon-like peptide-1 receptor agonist (GLP-1 RA) in a single injection for the treatment of patients with type 2 diabetes. The two types of FRC products contain different concentrations and mixing ratios of basal insulin and GLP-1 RA. Both products demonstrated satisfactory blood glucose control throughout the day, with less hypoglycemia and weight gain. However, few studies have examined the differences in the actions of the two formulations. Herein, we present a case of a 71-year-old man with pancreatic diabetes and significantly impaired intrinsic insulin secretion capacity, who demonstrated a marked difference in glycemic control following treatment with two different FRC formulations. Treatment with IDegLira, an FRC product, demonstrated suboptimal glucose control in the patient. However, after a change in therapy to another FRC product, IGlarLixi, his glucose control markedly improved, even with a decrease in the injection dose. This difference could have been due to lixisenatide, a short-acting GLP-1RA contained in IGlarLixi, which exerts a postprandial hypoglycemic effect irrespective of intrinsic insulin secretion capacity. In conclusion, IGlarLixi has the potential to achieve good fasting and postprandial glucose control with a once-daily injection, even in patients with type 2 diabetes who have a reduced intrinsic insulin secretion capacity. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13340-023-00621-5.

  Jul. 2023, Diabetology international, 14(3) (3), 294 - 297, English, Domestic magazine


• Panhypopituitarism Mimicking Acute Coronary Syndrome.

  Yoshito Sugi, Kenta Mori, Takashi Kobayashi, Naoki Arai, Mitsumasa Okano, Naokazu Muramae, Toshihiko Oshita, Kazunori Otsui, Kazuhiko Sakaguchi

  A 59-year-old man suspected of having myocardial infarction with sinus bradycardia, a decreased blood pressure, and ST-change on an electrocardiogram was referred to our hospital's emergency department. Emergent coronary angiography revealed no significant findings. However, the patient experienced shock and required intensive care. Curiosity rose when his urination volume was not disturbed; we suspected hormonal abnormalities. A hormonal examination and imaging analysis revealed panhypopituitarism caused by a Rathke's cyst. Appropriate hormonal replacement therapy improved his symptoms and led to normalization of his electrocardiogram findings. Acute coronary syndrome (ACS) is a fatal disease; however, clinicians must not discount panhypopituitarism, as it may mimic ACS symptoms.

  Feb. 2023, Internal medicine (Tokyo, Japan), 62(4) (4), 559 - 564, English, Domestic magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Hemophagocytic Syndrome-Like Tuberculosis-Immune Reconstitution Inflammatory Syndrome After the Initiation of Hepatic Tuberculosis Treatment.

  Serina Nakamura, Naokazu Muramae, Akira Fujisawa, Noriaki Yasuda, Mitsumasa Okano, Kenta Mori, Kazunori Otsui, Kazuhiko Sakaguchi

  A 25-year-old Nepalese woman was referred to our hospital because of fever and intermittent abdominal pain with inguinal lymphadenopathy, which had lasted for several months. A liver biopsy of the positron emission tomography-positive lesion led to a diagnosis of hepatic tuberculosis. After the initiation of antituberculosis treatment, her symptoms resolved. However, 11 days after treatment initiation, chest and back pain, high-grade fever, and vomiting appeared and gradually worsened. She developed anemia and her serum ferritin level was elevated. Hemophagocytic syndrome due to the initial deterioration of tuberculosis was suspected and steroid therapy was initiated with the continuation of the antituberculosis drugs. Thereafter, the patient's condition improved remarkably.

  May 2022, Cureus, 14(5) (5), e24644, English, International magazine

  Link to Kobe Univ. Repository (Kernel)


• Acute Onset of Remitting Seronegative Symmetrical Synovitis With Pitting Edema (RS3PE) Two Weeks After COVID-19 Vaccination With mRNA-1273 With Possible Activation of Parvovirus B19: A Case Report With Literature Review.

  Hiroto Arino, Naokazu Muramae, Mitsumasa Okano, Kenta Mori, Kazunori Otsui, Kazuhiko Sakaguchi

  Remitting seronegative symmetrical synovitis with pitting edema (RS3PE) is a rare clinical entity characterized by "remitting," "seronegative," and "symmetrical" synovitis with pitting edema on the dorsum of the hands and feet. Although rheumatic or malignant diseases are diseases that are known to coexist with RS3PE, other factors such as medication, infection, and vaccination have been reported to be associated with RS3PE. Here, we present a case of RS3PE syndrome that satisfied all four diagnostic criteria of RS3PE (pitting edema in the limbs, acute onset, age ≥ 50 years, and/or rheumatoid factor negativity) after mRNA-1273 SARS-CoV-2 vaccination.

  May 2022, Cureus, 14(5) (5), e24952, English, International magazine

  Link to Kobe Univ. Repository (Kernel)


• Nontraumatic bladder rupture: The images before and after.

  Mitsumasa Okano, Toshihiko Oshita, Kazunori Otsui, Kazuhiko Sakaguchi

  We should consider the complication of bladder rupture for patients with worsened abdominal pain and inability to pass urine following acute cystitis. A CT scan is a useful first-line modality when evaluating for a suspected bladder rupture.

  Jun. 2021, Clinical case reports, 9(6) (6), e04312, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• In Vivo Imaging of Venous Thrombus and Pulmonary Embolism Using Novel Murine Venous Thromboembolism Model.

  Mitsumasa Okano, Tetsuya Hara, Makoto Nishimori, Yasuhiro Irino, Seimi Satomi-Kobayashi, Masakazu Shinohara, Ryuji Toh, Farouc A Jaffer, Tatsuro Ishida, Ken-Ichi Hirata

  This work established a new murine venous thromboembolism (VTE) model. This model has multiple novel features representing clinical VTE that include the following: 1) deep venous thrombosis (DVT) was formed and extended in the long axis of femoral/saphenous vein; 2) thrombus was formed in a venous valve pocket; 3) deligation of suture-induced spontaneous pulmonary emboli of fibrin-rich DVT; and 4) cardiac motion-free femoral/saphenous vein allowed high-resolution intravital microscopic imaging of fibrin-rich DVT. This new model requires only commercially available epifluorescence microscopy. Therefore, this model has significant potential for better understanding of VTE pathophysiology.

  Apr. 2020, JACC. Basic to translational science, 5(4) (4), 344 - 356, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Association between abnormal myocardial scintigraphy findings and long-term outcomes for elderly patients 85 years or older: a retrospective cohort study.

  Takao Kato, Mitsumasa Okano, Yoshizumi Haruna, Moriaki Inoko

  BACKGROUND: Normal findings of cardiac scintigraphy predict good outcomes. However, a paucity of the data exists for elderly patients 85 years or older. In the present study, we aimed to demonstrate the association between the abnormal findings of cardiac scintigraphy and the risk of all cause death in patients 85 years or older. METHODS: We enrolled 143 consecutive patients 85 years or older with known or suspected coronary artery disease who underwent stress scintigraphy under adenosine or an exercise test and a 99mTechnetium (Tc)-labeled tracer or thallium 201 (201Tl), dual tracer rest scintigraphy using 201Tl and 123I-β-methyl iodophenyl pentadecanoic acid (123I-BMIPP), or 123I-BMIPP single tracer scintigraphy. Ischemia was defined by an induced perfusion abnormality according to a provocation test with recovery at rest or decreased uptake of 123I-BMIPP despite normal perfusion at rest. Infarction was defined by perfusion abnormalities assessed by images at rest on 201Tl or 99mTc-labeled tracer. We defined these findings as abnormal when at least one of these aforementioned characteristics was observed. RESULTS: Patients in the abnormal findings group (N = 62) were more likely to have undergone prior coronary angiography and to have decreased ejection fraction than those in the normal findings group (N = 81). The median follow-up duration was 797 days (interquartile range, 635-1045 days), with follow-up rates of 90% at 1 year and 73% at 2 years. The 2-year mortality rate were significantly higher in the abnormal findings group than in the normal findings group (26.8% vs. 10.9%; p = 0.01). The risk of abnormal findings relative to normal findings remained significant for the mortality (adjusted hazard ratio, 5.99; 95% CI, 1.37-42.8; P = 0.015). CONCLUSION: Abnormal myocardial scintigraphy findings were associated with the increased risk for mortality, even for patients 85 years or older.

  Nov. 2019, BMC cardiovascular disorders, 19(1) (1), 246 - 246, English, International magazine

  Scientific journal


• Species differences in lipoprotein lipase and hepatic lipase activities: comparative studies of animal models of lifestyle-related diseases.

  Norie Kimura, Akio Kikumori, Daisuke Kawase, Mitsumasa Okano, Kumiko Fukamachi, Tatsuro Ishida, Katsuyuki Nakajima, Masashi Shiomi

  Lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) have an important role in lifestyle-related diseases. To evaluate species differences, we compared LPL and HTGL activities in different animal models of lifestyle-related diseases using the same assay kit. Normal animals (JW rabbits, ICR mice, and SD rats), a hypercholesterolemic animal model (WHHLMI rabbits), and obese animal models (KK-Ay mice and Zucker fatty rats) fed standard chow were used in this study. Plasma was prepared before and after an intravenous injection of heparin sodium under fasting and feeding. LPL and HTGL activities were measured with the LPL/HTGL activity assay kit (Immuno-Biological Laboratories) using an auto-analyzer. Only in mice, high HTGL activity was observed in pre-heparin plasma. In normal animals, LPL and HTGL activities were high in ICR mice and SD rats but low in JW rabbits. Compared to normal animals, LPL activity was high in Zucker fatty rats and WHHLMI rabbits at both fasting and feeding, while LPL activity after feeding was low in KK-Ay mice. HTGL activity was higher in fasted and fed WHHLMI rabbits and fasted Zucker fatty rats, but was lower in fed KK-Ay mice. Gender difference was observed in HTGL activity in SD rats and LPL activity in WHHLMI rabbits but not in ICR mice. In conclusion, this simple assay method was effective for measuring LPL and HTGL activities of experimental animals, and the activities are highly regulated depending on animal species, animal models, feeding/fasting conditions and genders.

  Aug. 2019, Experimental animals, 68(3) (3), 267 - 275, English, Domestic magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Acute Pulmonary Hypertension Crisis after Adalimumab Reduction in Rheumatoid Vasculitis.

  Gentaro Yamasaki, Mitsumasa Okano, Kazuhiko Nakayama, Naoe Jimbo, Sho Sendo, Naoki Tamada, Kenta Misaki, Yuto Shinkura, Kenichi Yanaka, Hidekazu Tanaka, Kengo Akashi, Akio Morinobu, Hiroshi Yokozaki, Noriaki Emoto, Ken-Ichi Hirata

  Rheumatoid vasculitis is a rare etiology for pulmonary hypertension (PH) in patients with connective tissue disease. We encountered a case of acute PH crisis in a case with rheumatoid vasculitis eight months after undergoing adalimumab reduction. Since no repetition of arthralgia occurred after the adalimumab reduction, we decided to not increase the dose of adalimumab. However, hemodynamic collapse thereafter developed and even though steroid pulse therapy was administered, the patient nevertheless died. The autopsy showed clusters of acute and chronic inflammation around the remodeled pulmonary arteries along with micro-thrombi in the vessel lumen. We should consider the possibility of critical worsening of PH as a phenotype of vasculitis related to immunosuppressive therapy reduction.

  2019, Internal medicine (Tokyo, Japan), 58(4) (4), 593 - 601, English, Domestic magazine

  Scientific journal


• The optimization of iloprost inhalation under moderate flow of oxygen therapy in severe pulmonary arterial hypertension

  Kazuhiko Nakayama, Noriaki Emoto, Naoki Tamada, Mitsumasa Okano, Yuto Shinkura, Kenichi Yanaka, Hiroyuki Onishi, Mana Hiraishi, Shinichiro Yamada, Hidekazu Tanaka, Toshiro Shinke, Ken-Ichi Hirata

  SAGE Publications Ltd, Oct. 2018, Pulmonary Circulation, 8(4) (4), English

  Scientific journal


• Reversible Parkinsonism and Multiple Cerebral Infarctions after Pulmonary Endarterectomy in a Patient with Antiphospholipid Syndrome.

  Mitsumasa Okano, Kazuhiko Nakayama, Naoki Tamada, Yuto Shinkura, Ken-Ichi Yanaka, Hiroyuki Onishi, Hidekazu Tanaka, Toshiro Shinke, Hiroshi Tanaka, Yutaka Okita, Noriaki Emoto, Ken-Ichi Hirata

  Antiphospholipid syndrome (APS) is a cause of chronic thromboembolic pulmonary hypertension (CTEPH) and it is associated with an increased risk of postoperative neurological complications. We experienced a case of reversible parkinsonism after pulmonary endarterectomy (PEA) and subsequent multiple cerebral infarctions under standard anticoagulation therapy in a patient with CTEPH associated with APS. Strict management using a combination of antiplatelet and anticoagulation therapy should be considered in patients with a high titer of triple antiphospholipid antibodies in the perioperative period. We should be aware of the high risk of postoperative neurologic manifestations in patients with APS.

  Jul. 2018, Internal medicine (Tokyo, Japan), 57(14) (14), 2019 - 2023, English, Domestic magazine

  Scientific journal


• Progression of calcific aortic valve sclerosis in WHHLMI rabbits.

  Tetsuya Hara, Norie Tsukada, Mitsumasa Okano, Tatsuro Ishida, Ken-Ichi Hirata, Masashi Shiomi

  BACKGROUND AND AIMS: Aortic valve stenosis (AS) is the most common valvular heart disease and can be life-threatening. The pathogenesis of aortic valve calcification remains largely unknown, primarily due to the lack of an adequate animal model. The high-cholesterol diet-induced AS model in rabbits is one of the established models, but it has the significant limitation of liver dysfunction leading to low survival rates. We hypothesized that a myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHLMI) rabbit, an animal model of familial hypercholesterolemia and atherosclerosis, is a useful animal model of AS. METHODS: WHHLMI rabbits, aged 20 months and 30 months (n = 19), and control Japanese White rabbits (n = 4), aged 30 months, were used and evaluated by echocardiography under anesthesia. Pathological evaluation and quantitative analyses by polymerase chain reaction (PCR) were also performed. RESULTS: The lipid profile was similar between 20 months and 30 months. Two rabbits died due to spontaneous myocardial infarction during the study. Thirty-month-old WHHLMI rabbits exhibited significantly smaller aortic valve area (0.22 ± 0.006 cm2vs. 0.12 ± 0.01 cm2, p < 0.05) and higher maximal transvalvular pressure gradient (7.0 ± 0.32 vs. 9.9 ± 0.95 mmHg, p < 0.05) than 20 month-old rabbits. Macroscopic examination of excised aortic valves demonstrated thickened and degenerated valve leaflets at 30 months. Histological evaluation confirmed thickened leaflets with calcified nodules at 30 months. Real-time PCR of resected aortic valve also showed increased expression level of calcification-related molecules including osteopontin, Sox9, Bmp2, RANKL, osteoprotegerin, and Runx2 (p < 0.05 each) in 30-month-old rabbits. CONCLUSIONS: WHHLMI rabbits may be useful models of early-stage AS in vivo.

  Jun. 2018, Atherosclerosis, 273, 8 - 14, English, International magazine

  Scientific journal


• 新規マウス血栓モデルによる赤血球凝集主体のDVT生体イメージング

  岡野光真, Hara Tetsuya, 渡部晃一, 尾下寿彦, 津田成康, 入野康宏, 小林成美, 篠原正和, 杜隆嗣, 石田達郎, 平田健一

  日本臨床分子医学会, Apr. 2018, 日本臨床分子医学会学術総会プログラム・抄録集 55回, 55回, 58 - 58, Japanese


• 再構成HDLのphospholipid組成はその抗炎症作用に影響する(Phospholipid Composition of Reconstitute High Density Lipoproteins Affects Their Anti-inflammatory Effects)

  岡野 光真, 入野 康宏, 篠原 正和, 田中 伸明, 尾下 寿彦, 津田 成康, 渡部 晃一, 森 健太, 原 哲也, 小林 成美, 杜 隆嗣, 石田 達郎, 平田 健一

  (一社)日本循環器学会, Mar. 2018, 日本循環器学会学術集会抄録集, 82回, PJ015 - 1, English


• Upfront triple combination therapy-induced pulmonary edema in a case of pulmonary arterial hypertension associated with Sjogren's syndrome.

  Kimikazu Takeuchi, Kazuhiko Nakayama, Mitsumasa Okano, Naoki Tamada, Hideya Suehiro, Yuto Shinkura, Kenichi Yanaka, Hiroyuki Onishi, Hidekazu Tanaka, Toshiro Shinke, Noriaki Emoto, Ken-Ichi Hirata

  Clinical efficacy of combination therapy using vasodilators for pulmonary arterial hypertension (PAH) is well established. However, information on its safety are limited. We experienced a case of primary Sjogren's syndrome associated with PAH where the patient developed pulmonary edema immediately after the introduction of upfront triple combination therapy. Although the combination therapy successfully stabilized her pre-shock state, multiple ground glass opacities (GGO) emerged. We aborted the dose escalation of epoprostenol and initiated continuous furosemide infusion and noninvasive positive pressure ventilation (NPPV), but this did not prevent an exacerbation of pulmonary edema. Chest computed tomography showing diffuse alveolar infiltrates without inter-lobular septal thickening suggests the pulmonary edema was unlikely due to cardiogenic pulmonary edema and pulmonary venous occlusive disease. Acute respiratory distress syndrome was also denied from no remarkable inflammatory sign and negative results of drug-induced lymphocyte stimulation tests (DLST). We diagnosed the etiological mechanism as pulmonary vasodilator-induced trans-capillary fluid leakage. Following steroid pulse therapy dramatically improved GGO. We realized that overmuch dose escalation of epoprostenol on the top of dual upfront combination poses the risk of pulmonary edema. Steroid pulse therapy might be effective in cases of vasodilator-induced pulmonary edema in Sjogren's syndrome associated with PAH.

  2018, Respiratory medicine case reports, 23, 55 - 59, English, International magazine

  Link to Kobe Univ. Repository (Kernel)


• Targeted Disruption of JCAD (Junctional Protein Associated With Coronary Artery Disease)/KIAA1462, a Coronary Artery Disease-Associated Gene Product, Inhibits Angiogenic Processes In Vitro and In Vivo.

  Tetsuya Hara, Tomoko Monguchi, Noriko Iwamoto, Masaya Akashi, Kenta Mori, Toshihiko Oshita, Mitsumasa Okano, Ryuji Toh, Yasuhiro Irino, Masakazu Shinohara, Yui Yamashita, Go Shioi, Mikio Furuse, Tatsuro Ishida, Ken-Ichi Hirata

  OBJECTIVE: Recent genome-wide association studies newly identified the human KIAA1462 gene as a new locus for coronary artery disease. However, the function of the gene product, named JCAD (junctional protein associated with coronary artery disease), is unknown. Because JCAD is expressed at cell-cell junctions in endothelial cells, we hypothesized and tested whether JCAD regulates angiogenic processes in vitro and in vivo. APPROACH AND RESULTS: Cell culture experiments revealed impaired angiogenic ability (proliferation, migration, and cord formation) by the knockdown of JCAD with siRNA (P<0.05 versus control siRNA). We have generated mice lacking JCAD (mKIAA1462-/-) by gene-targeted deletion of JCAD to address in vivo angiogenic function. mKIAA1462-/- mice did not show morphological differences in development of retinal vasculature. Ex vivo aortic ring model demonstrated impaired neovascularization in aorta from mKIAA1462-/- mice than control wild-type mice (P<0.05). Tumor growth was assessed by monitoring tumor volume after the subcutaneous injection of melanoma, LLC (Lewis lung carcinoma), and E0771 cells into the mice. mKIAA1462-/- mice exhibited significantly smaller tumor volume compared with wild-type mice (P<0.001). Histological assessment of the tumor exhibited less smooth muscle actin-positive neovascularization determined by CD31-positive vascular structure in tumor of mKIAA1462-/- mice than wild-type mice, indicating that knockdown of JCAD inhibited the vascular maturation in pathological angiogenic process. CONCLUSIONS: These in vitro and in vivo studies suggest that JCAD has a redundant functional role in physiological angiogenesis but serves a pivotal role in pathological angiogenic process after birth.

  Sep. 2017, Arteriosclerosis, thrombosis, and vascular biology, 37(9) (9), 1667 - 1673, English, International magazine

  Scientific journal


• Patterns of ECG changes during occlusion of the left circumflex coronary artery

  Okano Mitsumasa, Kato Takao, Inoko Moriaki

  Background: The diagnosis of acute coronary syndrome caused by a culprit lesion in the left circumflex coronary artery (LCX) is still difficult. Purpose: The purpose of this study was to characterize the electrocardiographic (ECG) changes during the occlusion of the LCX in patients who underwent a scheduled percutaneous coronary intervention (PCI). We tested the hypothesis that the ischemic area estimated from the ECG changes according to Cabrera’s ECG sequences matches the perfusion area assessed with coronary angiography (CAG). Methods: We retrospectively evaluated 20 patients who underwent a scheduled PCI of the LCX because of a nonobstructive stenotic lesion, at our hospital between April 2013 and February 2014. We compared the ECG data taken before PCI and those taken during the occlusion of the LCX, and recorded the ECG changes according to the Cabrera sequence. The perfusion area was determined with CAG. The data of ECG changes and perfusion area were once de-identified and assessed by two senior cardiologists who were blinded to the results. Results: A total of 20 patients were included in these analyses. Fourteen patients (70%) showed changes in the I and aVL leads. Five patients (25%) showed changes in II, III, and aVF leads. Nine patients (45%) showed changes in the aVR lead. The perfusion areas estimated according to ECG changes were matched in 11 of 14 patients with ECG changes, whereas 6 patients did not show ECG changes. The positive predictive value was 0.76 (95% confidence interval: 0.652-0.786), and the negative predictive value was 1.000 (95% confidence interval: 0.687-1.000). Conclusions: Balloon occlusion of the LCX showed a variety of ECG changes. The perfusion area estimated from the ECG changes according to the Cabrera sequence was matched with the perfusion area assessed with CAG.

  The Japanese Coronary Association, 2016, Journal of the Japanese Coronary Association, 23(1) (1), 6 - 11, Japanese


• Incidence and Predictors of Catheterization-Related Cerebral Infarction on Diffusion-Weighted Magnetic Resonance Imaging.

  Yusuke Morita, Takao Kato, Mitsumasa Okano, Kanae Suu, Masahiro Kimura, Eri Minamino-Muta, Eisaku Nakane, Toshiaki Izumi, Shoichi Miyamoto, Tetsuya Haruna, Koji Ueyama, Moriaki Inoko

  Introduction. The aim of this study was to examine the incidence and risk factors of catheterization-related CI in the contemporary era, using diffusion-weighted magnetic resonance imaging. Methods. We retrospectively analyzed consecutive 84 patients who underwent MRI (magnetic resonance imaging) after 2.81 ± 2.4 days (mean ± SD) of catheterization via aortic arch. We categorized the patients by the presence or absence of acute CI determined by diffusion-weighted MRI and analyzed the incidence and predictors. Results. Of 84 patients that underwent MRI after catheterization, acute CI was determined in 27 (32.1%) patients. In univariate analysis, dyslipidemia, age, coronary artery disease, antiplatelet agents, number of catheters used, urgent settings, and interventional procedures were significantly different. Multivariate analysis revealed dyslipidemia (odds ratio [OR], 4.46; 95% confidence interval [CI], 1.41-16.03; p = 0.01), higher age (OR, 1.09; 95% CI, 1.007-1.19; p = 0.03), and the number of catheters used (OR, 2.21; 95% CI, 1.21-4.36; p = 0.01) as independent predictors of the incidence of catheterization-related acute CI. Conclusions. Dyslipidemia, higher age, and number of catheters used were independent predictors for acute CI after catheterization. These findings imply that managing dyslipidemia and comprehensive planning to minimize the numbers of catheters are important.

  2016, BioMed research international, 2016, 6052125 - 6052125, English, International magazine

  Scientific journal


• Effort angina with an anomalous origin of the left circumflex coronary artery.

  Mitsumasa Okano, Takao Kato, Moriaki Inoko

  Nov. 2014, BMJ case reports, 2014, English, International magazine

  Scientific journal


• A case of polymorphic ventricular tachycardia after coronary bypass surgery for ischemic cardiomyopathy

  Kimura Masahiro, Haruna Tetsuya, Okano Mitsumasa, Su Kanae, Morita Yusuke, Iida Jyun, Minamino Eri, Kato Takao, Nakane Eisaku, Morishima Manabu, Miyamoto Shouichi, Izumi Toshiaki, Ueyama Koji, Inoko Moriaki

  46歳男性, 既往に前壁OMI. 3枝病変による不安定狭心症に対し, CABG施行. 術後6日後よりNarrow QRS PVCを契機に, QRS軸の変化する多形性VTが出現した. 各種治療無効でVT stormとなり, 術後13日目にtriggered PVCを指標に, アブレーションを施行. 低電位領域を左室内apex～midの前側壁に認めた. 同部位辺縁で, アブレーションカテーテル (Abl) 電極にて, 近位から遠位への興奮順序 (sequence) を伴うPurkinje電位記録部位においてSpike-QRS 56msの潜時を伴うPerfect mapを得た. 同部位で, 多形性VTの自然発作が記録され, Poly VT時, Abl電極でPurkinje電位を認め, 体表面QRSに64ms先行していた. 多形性VT中, Purkinje電位間隔の変動が心室電位間隔の変動に先行し, Abl電極のsequence変化とQRS軸の変化が同期して出現した. 同部位の通電開始で多形性VTが出現し, 通電中に停止した. 多形性VTの維持機序に障害Purkinje網でのリエントリ関与が示唆されたので, 報告する.

  Japan Heart Foundation, 2014, Shinzo, 46(3) (3), S3_212 - S3_218, Japanese

• A case of glucose tolerance assessment before and after Sacubitril/Valsartan (ARNI) administration

  坂口一彦, 西坂まり華, 森田靖子, 山田倫子, 木村真希, 畑澤圭子, 岡野光真, 森健太, 乙井一典

  2023, 日本高血圧学会総会プログラム・抄録集(CD-ROM), 45th


• Establishment of Femoral Vein Ligation Model: a New DVT Imaging Model

  Tetsuya Hara, Mitsumasa Okano, Makoto Nishimori, Koichi Watanabe, Yasuhiro Irino, Seimi Kobayashi, Masakazu Shinohara, Ryuji Toh, Tatsuro Ishida, Ken-ichi Hirata

  May 2019, ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 39, English

  Summary international conference


• 国際交流 実績報告 Novel murine deep venous thrombosis (DVT) model enables real-time in vivo imaging of clinically relevant DVT formation

  岡野 光真

  福田記念医療技術振興財団, Dec. 2018, 福田記念医療技術振興財団情報, (31) (31), 67 - 70, Japanese


• Establishment of Novel Deep Venous Thrombosis Model Suitable for In Vivo Imaging.

  Mitsumasa Okano, Tetsuya Hara, Makoto Nishimori, Koichi Watanabe, Toshihiko Oshita, Yasuhiro Irino, Seimi Kobayashi, Masakazu Shinohara, Ryuji Toh, Tatsuro Ishida, Ken-ichi Hirata

  06 Nov. 2018, CIRCULATION, 138, English

  Summary international conference


• 85歳以上の高齢者における心筋シンチ結果と予後の関連性

  岡野光真, 加藤貴雄, 春名徹也, 猪子森明

  Apr. 2015, 第112回日本内科学会講演会, Japanese

  [Refereed]

  Summary national conference

• Venous Thrombosis in a Patient with Pseudo-Cushing Syndrome: a Case Report

  Mitsumasa Okano, Kazunori Otsui

  第90回日本循環器学会学術集会, Mar. 2026


• Successful Treatment of Toxic Multinodular Goiter with Atrial Fibrillation and Acute Heart Failure: a Case Report

  Mitsumasa Okano, Kenta Mori, Kazunori Otsui, Kazuhiko Sakaguchi

  JCS2024, Mar. 2024


• 症候性の高Ca血症を伴う副甲状腺腺腫に対して、内科的な全身管理後の手術で良好な経過を辿った超高齢の一例

  岡野光真, 合田建, 畑澤圭子, 森健太, 乙井一典, 安田尚史, 坂口一彦

  第34回日本老年医学会近畿地方会, Nov. 2023


• PET検査と肺動脈カテーテル生検が診断に有用だった子宮肉腫による肺腫瘍塞栓の一例

  岡野光真, 谷口悠, 畑澤圭子, 森健太, 乙井一典, 坂口一彦

  第6回日本腫瘍循環器学会学術集会, Sep. 2023


• CTEPH患者のDVTパターン解析

  岡野光真, 谷口悠, 乙井一典, 江本憲昭

  第8回日本肺高血圧・肺循環学会学術集会, Jun. 2023


• 腹部感染症に伴う上腸間膜静脈血栓症の症例検討

  岡野光真, 乙井一典, 森健太, 坂口一彦

  第120回日本内科学会総会, Apr. 2023


• Deep Vein Thrombosis Patterns in Chronic Thromboembolic Pulmonary Hypertension Patients

  Mitsumasa Okano, Yu Taniguchi, Keisuke Miwa, Keiko Sumimoto, Kenta Mori, Kazunori Otsui, Noriaki Emoto, Ken-ichi Hirata

  第86回日本循環器学会学術集会, Mar. 2022, English

  Poster presentation


• 門脈血栓症に対する抗凝固薬の使用経験

  岡野 光真, 乙井 一典, 森 健太, 坂口 一彦

  第118回日本内科学会総会・講演会, Apr. 2021


• Novel Deep Venous Thrombosis Model Enabled Visualization of Thrombus Formation and Organization Process in vivo.

  Mitsumasa Okano, Tetsuya Hara, Makoto Nishimori, Koichi Watanabe, Toshihiko Oshita, Yasuhiro Irino, Satomi-Kobayashi Seimi, Masakazu Shinohara, Ryuji Toh, Tatsuro Ishida, Kenichi Hirata

  第83回 日本循環器学会学術集会, Mar. 2019, 横浜


• Establishment of Novel Murine Deep Venous Thrombosis Model Suitable for in vivo Imaging

  Mitsumasa Okano, Tetsuya Hara, Makoto Nishimori, Koichi Watanabe, Toshihiko Oshita, Yasuhiro Irino, Seimi Kobayashi, Masakazu Shinohara, Ryuji Toh, Tatsuro Ishida, Ken-ichi Hirata

  American Heart Association Scientific Sessions 2018., Nov. 2018, English, シカゴ

  Oral presentation

• 血栓後症候群における予測因子の探索と静脈弁機能変化の検証

  岡野 光真

  日本学術振興会, 科学研究費助成事業 若手研究, 若手研究, 神戸大学, 01 Apr. 2022 - 31 Mar. 2024