SEARCH
Search Details
SASAYAMA DaisukeGraduate School of Agricultural Science / Department of Bioresource ScienceAssociate Professor
Research activity information
■ Award- Oct. 2016 日本作物学会, 日本作物学会 第242回講演会優秀発表賞, 水田雑草オモダカとウリカワの塊茎萌芽期の節間伸長の酸素欠乏および二酸化炭素による促進Others
- Oct. 2015 日本作物学会, 日本作物学会 第240回講演会優秀発表賞, アマゾン野生イネOryza glumaepatulaの深水適応Others
- CO2-Responsive CCT Protein (CRCT) is a transcription factor that positively regulates the expression of starch synthesis-related genes in the vegetative organs of rice. CRCT interacts with 14-3-3 proteins. Yeast two-hybrid analysis showed that the CRCT with the S217A mutation was unable to interact with CRCT. This mutated CRCT was introduced into the CRCT knockout rice. The transcript of CRCT-S217A was detected significantly by qRT-PCR, but not by immunoblotting. Nevertheless, starch accumulation and the expression of starch synthesis-related genes in the leaf sheath of the transformants were higher than those of the CRCT knockout line. These results suggest the possibility that 14-3-3 proteins may stabilize CRCT through their interaction, while free unstable CRCT can promote starch synthesis. CRCT was shown to be highly expressed in the vascular bundle and is hypothesized to move to the starch-accumulating storage parenchyma cells in leaf sheaths. To investigate this hypothesis, we generated transgenic lines expressing CRCT ectopically in the CRCT knockout line background. Transgenic lines expressing CRCT using Sucrose transpor1 promoter, known to express specifically in vascular tissues, and the Chlorophyll a/b binding protein promoter, known to express highly in photosynthetic tissues, showed recovered starch accumulation in the leaf sheath. These results suggest that CRCT can move from an unspecified cell to storage parenchyma cells via the vascular bundle to regulate starch synthesis. This study can provide new insight into the regulation mechanism of starch biosynthesis in the vegetative organs.Jul. 2026, Plant science : an international journal of experimental plant biology, 368, 113157 - 113157, English, International magazineScientific journal
- May 2026, Tropical Plant Biology, 19, 15, EnglishResponses of the Australian Wild Rice Oryza meridionalis to Complete Submergence[Refereed]Scientific journal
- Abstract CO2-Responsive CCT Protein (CRCT) is a transcription factor that regulates the expression of starch synthesis-related genes, and consequently starch content in the vegetative organs of rice. Two CRCT homologues, AtCRCT1 and AtCRCT2, were identified in the genome of Arabidopsis thaliana. AtCRCT1 and AtCRCT2 were expressed in most organs of the plant, and AtCRCT2 in particular was expressed in their vascular bundles. The expression of these genes was markedly upregulated by sucrose treatment and tended to increase under elevated CO2 condition. The overexpression or knockout of these genes did not have a notable effect on the growth of Arabidopsis. On the other hand, the expression of some starch synthesis-related genes, such as ADP-glucose pyrophosphorylase large subunit3 (AtAPL3) and glucose 6-phosphate/Pi transporter2 (AtGPT2) was significantly upregulated in the overexpression lines. Accordingly, the expression of AtAPL3 and AtGPT2 in the double knockout line was significantly lower than that in wild-type (Col-0) under sucrose treatment. In addition, the starch content of the double knockout line at the end of the night was slightly lower than that of Col-0. Yeast two-hybrid and BiFC analyses demonstrated that both AtCRCT1 and AtCRCT2 interact with Growth Regulation Factor 7, a 14-3-3 protein. These results suggest that AtCRCT1 and AtCRCT2 have similar molecular and physiological functions to CRCT in rice and regulate the expression of some starch synthesis-related genes. However, their effects are limited and cannot markedly affect starch content, unlike CRCT in rice.Oxford University Press (OUP), Jan. 2026, Plant and Cell PhysiologyScientific journal
- Corresponding, Elsevier BV, Sep. 2025, Crop and Environment, 4(3) (3), 168 - 172, English[Refereed]Scientific journal
- Lead, Elsevier BV, Mar. 2025, Rice Science, English[Refereed]Scientific journal
- Informa UK Limited, Apr. 2024, Plant Production Science, 27(3) (3), 221 - 228[Refereed]Scientific journal
- Informa UK Limited, Jul. 2023, Plant Production Science, 26(3) (3), 273 - 286, English[Refereed]Scientific journal
- Lead, Informa UK Limited, Jan. 2023, Plant Production Science, 26(1) (1), 1 - 11, English[Refereed]Scientific journal
- Lead, Informa UK Limited, Jul. 2022, Plant Production Science, 25(3) (3), 350 - 358, English[Refereed]Scientific journal
- Jul. 2022, Journal of Crop Research, 67, 51 - 55, EnglishControl of growth direction in crown roots in rice germinated seedlings by light[Refereed]Scientific journal
- Lead, Jul. 2022, Journal of Crop Research, 67, 35 - 40, EnglishInternodal elongation under submergence in Oryza sativa/Oryza glumaepatula introgression lines[Refereed]Scientific journal
- Oxford University Press (OUP), Mar. 2022, Journal of Experimental Botany, 73(9) (9), 3030 - 3043, English, International magazine
Abstract Triacylglycerols (TAGs) are the major component of plant storage lipids such as oils. Acyl-CoA:diacylglycerol acyltransferase (DGAT) catalyzes the final step of the Kennedy pathway, and is mainly responsible for plant oil accumulation. We previously found that the DGAT activity of Vernonia DGAT1 was distinctively higher than that of Arabidopsis DGAT1 and soybean DGAT1 in a yeast microsome assay. In this study, the DGAT1 cDNAs of Arabidopsis, Vernonia, soybean, and castor bean were introduced into Arabidopsis. All Vernonia DGAT1 expressing lines showed a significantly higher oil content (49% mean increase compared to wild-type) followed by soybean and castor bean. Most Arabidopsis DGAT1 over-expressing lines did not show a significant increase. In addition to these four DGAT1s, sunflower, Jatropha, and sesame DGAT1 genes were introduced into a TAG biosynthesis defective yeast mutant. In the yeast expression culture, DGAT1s from Arabidopsis, castor bean, and soybean only slightly increased the TAG content; however, DGAT1s from Vernonia, sunflower, Jatropha, and sesame increased TAG content more than 10 times than the former three DGAT1s. Three amino acid residues were characteristically common in the latter four DGAT1s. Using soybean DGAT1, these amino acid substitutions were performed by site-directed mutagenesis and substantially increased the TAG content.[Refereed]Scientific journal - Lead, Jan. 2022, Plant Production Science, 25(1) (1), 70 - 77, English[Refereed]Scientific journal
- Aug. 2021, PLANT CELL AND ENVIRONMENT, 44(8) (8), 2480 - 2493, English[Refereed]Scientific journal
- Jun. 2020, Journal of Crop Research, 65, 37 - 41, EnglishResponses to Complete Submergence in African Rice (Oryza glaberrima)[Refereed]Scientific journal
- Informa UK Limited, Apr. 2019, Plant Production Science, 22(2) (2), 296 - 300[Refereed]Scientific journal
- Sep. 2018, Photosynthesis Research, 137(3) (3), 465 - 474, English[Refereed]Scientific journal
- Lead, Springer Science and Business Media LLC, May 2018, Plant Growth Regulation, 85(1) (1), 123 - 132[Refereed]Scientific journal
- Lead, Dec. 2016, WEED BIOLOGY AND MANAGEMENT, 16(4) (4), 147 - 156, English[Refereed]Scientific journal
- Sep. 2016, FRONTIERS IN PLANT SCIENCE, 7, 1479, English[Refereed]Scientific journal
- Mar. 2015, WEED BIOLOGY AND MANAGEMENT, 15(1) (1), 20 - 26, English[Refereed]Scientific journal
- Lead, Feb. 2015, JOURNAL OF PLANT PHYSIOLOGY, 174, 49 - 54, English[Refereed]Scientific journal
- Sep. 2014, PLANTA, 240(3) (3), 459 - 469, English[Refereed]Scientific journal
- Lead, Nov. 2013, BMC PLANT BIOLOGY, 13(1) (1), 189, English[Refereed]Scientific journal
- Sep. 2013, JOURNAL OF PLANT PHYSIOLOGY, 170(13) (13), 1158 - 1164, English[Refereed]Scientific journal
- May 2013, PLANT BIOTECHNOLOGY, 30(2) (2), 111 - 118, English[Refereed]Scientific journal
- Lead, Dec. 2011, PLANT BIOTECHNOLOGY, 28(5) (5), 463 - 470, English[Refereed]Scientific journal
- Sep. 2011, Tropical Agriculture and Development, 55, 113 - 117, EnglishInvolvement of Plant Hormones in the Gravitropic Response of Floating Rice Pulvini and Effect of Submergence on the Response[Refereed]Scientific journal
- Sep. 2011, Tropical Agriculture and Development, 55, 108 - 112, EnglishCharacterization of gravitropic response of leaf-sheath pulvini in floating rice[Refereed]Scientific journal
- Lead, Jan. 2011, JOURNAL OF PLANT PHYSIOLOGY, 168(2) (2), 121 - 127, English[Refereed]Scientific journal
- Mar. 2010, PLANT SCIENCE, 178(3) (3), 245 - 250, English[Refereed]Scientific journal
- Lead, Jan. 2009, PLANT GROWTH REGULATION, 57(1) (1), 79 - 88, English[Refereed]Scientific journal
- Dec. 2007, JOURNAL OF PLANT PHYSIOLOGY, 164(12) (12), 1683 - 1687, English[Refereed]Scientific journal
- Involvement of acid-induced growth and expansin action in the internodal elongation of submerged floating riceFloating or deepwater rice (Oryza sativa L.) responds to submergence by rapid internodal elongation. The involvement of acid-induced growth and the pH-dependent wall-loosening protein expansin in such internodal elongation was examined. Living internodal sections excised from floating rice stem segments that had been submerged showed significant enhancement of their growth inLead, 日本熱帯農業学会, Sep. 2007, Japanese Journal of Tropical Agriculture, 51(3) (3), 95 - 101, English[Refereed]Scientific journal
- May 2012, MOLECULES AND CELLS, 33(5) (5), 423 - 430, English[Refereed]Book review
- Growth in Internodes of Floating Rice: Hormonal Regulations and Cell Wall ModificationsFloating rice is the only crop that can be grown in flood plains because of the capacity to elongate internodes rapidly under conditions of flooding. The mechanisms of growth adaptation to flooding stress have been investigated from ecological, morphological, genetical, physiological, biochemical and molecular biological points of view. In this review, we mainly describe our rJan. 2009, Japanese Journal of Crop Science, 78, 1 - 8, Japanese[Refereed]Introduction scientific journal
- 日本作物学会第 256 回講演会, Sep. 2023, Japanese日本・世界のイネコアコレクションにおける幼植物体の低温ストレス耐性Poster presentation
- 日本作物学会第 256 回講演会, Sep. 2023, Japanese日本在来イネ・コアコレクションにおける幼苗期の高温耐性品種の探索Poster presentation
- 日本作物学会 第252回講演会, Sep. 2021, Japanese外生WRI1発現は種子油の含有量と脂肪酸組成に影響を及ぼすOral presentation
- 日本作物学会 第252回講演会, Sep. 2021, Japaneseエチレンに依存しない浮稲節間の深水下での伸長Oral presentation
- 10th Asian Crop Science Association Conference, Sep. 2021, EnglishSNORKELs and Deepwater Responses in the African Cultivated Rice Oryza glaberrimaOral presentation
- 10th Asian Crop Science Association Conference, Sep. 2021, EnglishAdaptive Responses to Flood in Wild Rice Species with Various Genomes Other Than AAOral presentation
- 10th Asian Crop Science Association Conference, Sep. 2021, EnglishDGAT1s from Different Plant Species Show Different Triacylglycerol Biosynthesis ActivitiesPoster presentation
- 10th Asian Crop Science Association Conference, Sep. 2021, EnglishHighly Active Vernonia galamensis DGAT1 Can Effectively Increase Oil Levels in Yeast, Soybean and ArabidopsisOral presentation
- 10th Asian Crop Science Association Conference, Sep. 2021, EnglishA Challenge for the Improvement of Photosynthetic Capacity by the Introduction of C4-Like Rubisco in Rice
- 近畿作物・育種研究会 第190回例会, Jun. 2021, Japanese浮稲性遺伝子SNORKEL1/2を持たない浮稲品種の特徴Oral presentation
- 近畿作物・育種研究会 第 189 回例会, Dec. 2020, JapaneseOryza sativa/Oryza glumaepatula染色体置換系統の浮稲性の解析Oral presentation
- 日本作物学会 第250回講演会, Sep. 2020, Japaneseアフリカイネ Oryza glaberrima における冠水に対する応答Oral presentation
- 日本作物学会 第248回講演会, Sep. 2019, Japanese, とりぎん文化会館, Domestic conference高温前処理によるイネメソコチルの伸長促進反応 -植物ホルモンの関与と深播き耐性の改善-Poster presentation
- 日本作物学会 第248回講演会, Sep. 2019, Japanese, とりぎん文化会館, Domestic conferenceイネAAゲノム種の洪水適応 -Oryza longistaminataの洪水応答-Poster presentation
- 日本作物学会 第248回講演会, Sep. 2019, Japanese, とりぎん文化会館, Domestic conferenceAAタイプ以外のゲノムをもつ野生イネ種の洪水応答Poster presentation
- 第31回植物脂質シンポジウム, Nov. 2018, Japanese, 高知大学, Domestic conferenceVernonia galamensis DGAT1 can increase Arabidopsis seed oil level more than other DGAT1sPoster presentation
- The 14th International Symposium on Biocatalysis and Agricultural Biotechnology, Oct. 2018, English, Kuala Lumpur, Malaysia, International conferenceVernonia galamensis DGAT1 can increase Arabidopsis seed oil content more than other DGAT1sPoster presentation
- 日本作物学会第246回講演会, Sep. 2018, Japanese, 北海道大学農学部, Domestic conferenceAnalysis of Floating Response of South American Wild Rice Oryza glumaepatulaPoster presentation
- 日本作物学会第246回講演会, Sep. 2018, Japanese, 北海道大学農学部, Domestic conferenceStructure-Function Relationship of Hybrid Rubisco Consisting of Sorghum RbcS and Rice RbcLOral presentation
- 日本作物学会第246回講演会, Sep. 2018, Japanese, 北海道大学農学部, Domestic conferenceGrowth response of the African cultivated rice Oryza glaberrima and its wild ancestor Oryza barthii to floodingPoster presentation
- The 23rd International Symposium on Plant Lipids, Jul. 2018, English, Yokohama, Japan, International conferenceHighly active Vernonia galamensis DGAT1 can effectively increase oil levels in yeast, soybean and ArabidopsisPoster presentation
- 近畿作物・育種研究会 第185回例会, Jun. 2018, Japanese, Domestic conferenceアフリカイネ Oryza glaberrima とその祖先種 O. barthii の洪水に対する応答Oral presentation
- 日本作物学会講演会, 2018, Japanese, Domestic conferenceシロイヌナズナにおける CRCT オーソログの機能解析Poster presentation
- 日本作物学会講演会, 2018, Japanese, Domestic conferenceイネの Rubisco activase 様タンパク質 Rca2 は Rubisco activase の機能を阻害するPoster presentation
- 日本光合成学会講演会, 2018, Japanese, Domestic conferenceイネRubisco activase様タンパク質OsRca2はRubiscoの活性化を阻害するPoster presentation
- International Symposium on Photosynthesis and Chloroplast Biogenesis, 2018, English, Domestic conferenceFunctional analysis of Rubisco small subunit, OsRbcS1, that is not expressed in photosynthetic organs in ricePoster presentation
- 日本作物学会講演会, 2018, Japanese, Domestic conferenceC4植物の Rubisco 小サブユニットを高発現する形質転換イネの Rubisco 酵素特性Poster presentation
- The 7th Asian Symposium on Plant Lipid, Nov. 2017, English, Taipei, Taiwan, International conferenceHighly active Vernonia galamensis DGAT1 can effectively increase seed oil levels in soybean and ArabidopsisPoster presentation
- 日本作物学会第244回講演会, Sep. 2017, Japanese, 岐阜大学, Domestic conference浮稲性遺伝子と冠水耐性遺伝子を併せ持つイネ品種の深水成長応答と関連遺伝子の発現Poster presentation
- 日本作物学会第244回講演会, Sep. 2017, Japanese, 岐阜大学, Domestic conference南米野生イネOryza glumaepatulaの深水に対する応答とSNORKEL遺伝子の多様性Poster presentation
- 日本作物学会第244回講演会, Sep. 2017, Japanese, 岐阜大学, Domestic conference深水環境下において浮稲節間の伸長を誘導する新規因子の探索Poster presentation
- 日本作物学会 第244回講演会, Sep. 2017, Japanese, 岐阜大学, Domestic conferenceDoes Vernonia DGAT1 show a higher activity than other DGAT1s in Arabidopsis seed?Poster presentation
- 第30回植物脂質シンポジウム, Sep. 2017, Japanese, お茶の水大学, Domestic conferenceシロイヌナズナにおけるベルノニアDGAT1の働きPoster presentation
- Plant Biology 2017, Jun. 2017, English, Honolulu, Hawaii, USA, Plant oils represent one of the world’s most important renewable resources. In addition to extensive food uses, vegetable oils also have value as renewable chemical derivatives and fuels, such as biodiesel. Worldwide production of plant oil has been increasing. Triacylglycerols (TAGs) are the major component of plant storage lipid. Acyl-CoA:diacylglycerol acyltransferase (DGAT), International conferenceVernonia DGAT1 can increase Arabidopsis seed oil content more than other DGAT1sPoster presentation
- 日本作物学会講演会, 2017, Japanese, Domestic conferenceマイクロアレイ法によるOsRbcS1発現抑制イネの遺伝子発現プロファイリングPoster presentation
- 農芸化学会関西支部会, 2017, Japanese, Domestic conferenceソルガム高活性型Rubiscoの小サブユニットを利用したイネの光合成能力の改良Oral presentation
- 農芸化学会関西支部会, 2017, Japanese, Domestic conferenceイネにおけるRubisco activase様タンパク質OsRca2の機能解析Oral presentation
- 日本作物学会 第242回講演会, Sep. 2016, Japanese, 龍谷大学瀬田キャンパス, Domestic conference浮稲性遺伝子と冠水耐性遺伝子を併せ持つイネ系統の深水伸長応答Poster presentation
- 日本作物学会 第242回講演会, Sep. 2016, Japanese, 龍谷大学瀬田キャンパス, Domestic conference水田雑草オモダカとウリカワの塊茎萌芽期の節間伸長の酸素欠乏および二酸化炭素による促進Oral presentation
- 日本作物学会 第242回講演会, Sep. 2016, Japanese, 龍谷大学瀬田キャンパス, Domestic conferenceエチレンとジベレリンが誘導する浮稲節間の伸長促進に及ぼす重力方向と過重力の影響Poster presentation
- 近畿作物・育種研究会 第181回例会, May 2016, Japanese, Domestic conference浮稲性遺伝子と冠水耐性遺伝子を併せ持つイネ系統の生理特性Oral presentation
- 近畿作物・育種研究会 第181回例会, May 2016, Japanese, 滋賀県立大学 サテライト教室, Domestic conference多年生水田雑草オモダカとウリカワの節間伸長はそれぞれ酸素欠乏と二酸化炭素によって促進されるOral presentation
- 日本作物学会 第240回講演会, Sep. 2015, Japanese, 信州大学長野 (工学) キャンパス, Domestic conference野生イネOryza altaおよびO. latifoliaの洪水適応Poster presentation
- 日本作物学会 第240回講演会, Sep. 2015, Japanese, 信州大学長野 (工学) キャンパス, Domestic conference浮稲における冠水耐性遺伝子SUB1Aの存在とその機能Oral presentation
- 日本作物学会 第240回講演会, Sep. 2015, Japanese, 信州大学長野 (工学) キャンパス, Domestic conferenceアマゾン野生イネOryza glumaepatulaの深水適応Oral presentation
- 近畿作物・育種研究会 第179回例会, May 2015, Japanese, 奈良県立橿原考古学研究所, Domestic conferenceアマゾン野生イネOryza glumaepatulaの浮稲性についてOral presentation
- 日本作物学会 第238回講演会, Sep. 2014, Japanese, 愛媛大学 城北キャンパス, Domestic conference浮稲茎葉部における重力屈性反応へのエクスパンシンの関与Poster presentation
- 近畿作物・育種研究会 第177回例会, Jul. 2014, Japanese, 神戸大学農学部, Domestic conference浮稲不定根の成長方向と光の関係Oral presentation
- 近畿作物・育種研究会 第177回例会, Jul. 2014, Japanese, 神戸大学農学部, Domestic conference浮稲茎葉部の重力屈性におけるエクスパンシンの関与Oral presentation
- 近畿作物・育種研究会 第177回例会, Jul. 2014, Japanese, 神戸大学農学部, Domestic conference浮稲におけるSUB1A遺伝子の存在とその分類Oral presentation
- 近畿作物・育種研究会 第177回例会, Jul. 2014, Japanese, 神戸大学農学部, Domestic conferenceアマゾン野生イネOryza grandiglumisの浮稲性と冠水耐性Oral presentation
- 11th Conference of the International Society for Plant Anaerobiosis, Oct. 2013, English, IRRI, Los Baños, Laguna, Philippines, Emergent plants Sagittaria trifolia and S. pygmaea are perennial Alismataceous weeds of paddy field. Those plants mainly propagate vegetatively by tubers. Their sprouting from tubers starts with irrigation for paddling of the rice fields and continues for several months. Sprouting finally leads to emergence of the shoot parts of those plants from the soil. In this study, we cha, International conferenceTuber sprouting and emergence of Sagittaria trifolia and S. pygmaea: their physiological traits and regulatory mechanismsPoster presentation
- 日本作物学会 第236回講演会, Sep. 2013, Japanese, 鹿児島大学 郡元キャンパス, Domestic conferenceEffect of gravity and light on growth direction in crown roots of floating ricePoster presentation
- 日本作物学会 第236回講演会, Sep. 2013, Japanese, 鹿児島大学 郡元キャンパス, Domestic conference低酸素環境が誘導する浮稲節間の伸長反応Poster presentation
- 近畿作物・育種研究会 第175回例会, Jul. 2013, Japanese, 近畿大学生物理工学部, Domestic conference浮稲節間の伸長に及ぼす重力の影響とオーキシンの関与Oral presentation
- 近畿作物・育種研究会 第175回例会, Jul. 2013, Japanese, 近畿大学生物理工学部, Domestic conferenceイネ葉枕の重力屈性は嫌気環境で促進されるがコムギとエンバク葉枕では阻害されるOral presentation
- 第54回日本植物生理学会年会, Mar. 2013, Japanese, 岡山大学, Domestic conferenceArabidopsis Raf-like MAPKKK, MAP3Kδ4 regulates plant growth.Oral presentation
- 4th International Auxin Meeting, Dec. 2012, English, Waikoloa Beach Marriott Resort & Spa, Hawai’i Island, USA, International conferenceLong-looped PIN-FORMEDs Require the Conserved M3 Phosphorylation Motif for Regulation of Their Intracellular Trafficking in ArabidopsisPoster presentation
- Korean Society for Molecular and Cellular Biology 2012 Annual Meeting, Oct. 2012, English, COEX, Seoul, KOREA, Domestic conferenceThe M3 Phosphorylation Code Has Been Functionally Conserved Among Long-looped PIN-FORMEDs for Regulation of Their Intracellular Trafficking in the Arabidopsis Root Hair CellPoster presentation
- 10th International Congress on Plant Molecular Biology, Oct. 2012, English, ICC, Jeju Island, Republic of Korea, International conferenceThe M3 Phosphorylation Code Has Been Functionally Conserved Among Long-looped PIN-FORMEDs for Regulation of Their Intracellular Trafficking in the Arabidopsis Root Hair CellPoster presentation
- 日本植物細胞分子生物学会 第30回大会, Aug. 2012, Japanese, 奈良先端科学技術大学院大学, Domestic conferenceシロイヌナズナMAP3Kδ4過剰発現体の塩ストレス耐性についてOral presentation
- 日本植物細胞分子生物学会 第30回大会, Aug. 2012, Japanese, 奈良先端科学技術大学院大学, Domestic conferenceシロイヌナズナMAP3Kδ4による枝分かれ制御についてOral presentation
- 日本作物学会 第232回講演会, Sep. 2011, Japanese, 山口大学農学部, Domestic conference浮稲節間細胞壁におけるエクスパンシンの反応性に及ぼすフェノール化合物と多糖の影響Poster presentation
- 日本植物細胞分子生物学会 第29回大会, Sep. 2011, Japanese, 九州大学箱崎キャンパス, Domestic conferenceシロイヌナズナMAPKKK(MAP3Kδ4)の過剰発現は成長促進とストレス耐性を付与するOral presentation
- 日本植物細胞分子生物学会 第29回大会, Sep. 2011, Japanese, 九州大学箱崎キャンパス, Domestic conferenceシロイヌナズナMAP3Kδ4の植物ホルモン応答性についてOral presentation
- 21st International Conference on Arabidopsis Research 2010, Jun. 2010, English, パシフィコ横浜, International conferenceRegulatory role of N-terminal region of MEKK1 on its protein kinase activityPoster presentation
- 21st International Conference on Arabidopsis Research 2010, Jun. 2010, English, パシフィコ横浜, International conferenceInvolvement of MAP3Kδ4 in growth regulation and stress signaling in Arabidopsis thaliana.Poster presentation
- 日本分子生物学会 第32回年会, Dec. 2009, Japanese, パシフィコ横浜, Domestic conferenceシロイヌナズナMEKK1の活性制御機構と基質特異性についてPoster presentation
- 日本分子生物学会 第32回年会, Dec. 2009, Japanese, パシフィコ横浜, Domestic conferenceArabidopsis thalianaの成長調節およびストレスシグナリングにおけるAtMAP3Kδ4の役割についてPoster presentation
- 日本植物細胞分子生物学会 第27回大会, Jul. 2009, Japanese, 日本大学生物資源科学部湘南キャンパス, Domestic conferenceシロイヌナズナMAPK AtMPK13の選択的スプライシングとその産物の機能についてOral presentation
- 日本作物学会 第226回講演会, Sep. 2008, Japanese, 神戸大学農学部, Domestic conference浮稲節間におけるエクスパンシンに対する細胞壁の反応性と壁構造との関係Poster presentation
- 日本作物学会 第226回講演会, Sep. 2008, Japanese, 神戸大学農学部, Domestic conference山口県の水田から見つかったスルホニルウレア抵抗性ミズマツバとアブノメOral presentation
- 種生物学会 第39回種生物シンポジウム, Nov. 2007, Japanese, 六甲山YMCA, Domestic conference浮稲の深水条件下での節間伸長におけるエクスパンシンの関与Poster presentation
- 9th Conference of the International Society for Plant Anaerobiosis, Nov. 2007, English, Matushima, Sendai, Japan, International conferenceInvolvement of expansins in internodal elongation of submerged floating ricePoster presentation
- 日本作物学会 第224回講演会, Sep. 2007, Japanese, 金沢大学角間キャンパス, Domestic conference浮稲節間の細胞壁におけるエクスパンシンに対する反応性とフェノール性化合物の沈着の関係Oral presentation
- 日本作物学会 第221回講演会, Mar. 2006, Japanese, 東京大学農学部, Domestic conference浮稲節間の伸長停止に伴うエクスパンシンの活性と細胞壁反応性の変化Oral presentation
- 近畿作物・育種研究会 第159回例会, Jul. 2005, Japanese, 神戸大学農学部, Domestic conference浮稲節間組織における酸が誘導する成長とエクスパンシンとの関係Oral presentation
- 日本作物学会 第216回講演会, Sep. 2003, Japanese, 島根大学生物資源科学部, Domestic conference浮稲節間の伸長に伴うエクスパンシンの活性と細胞壁反応性の変化Oral presentation
- 日本熱帯農業学会 第94回講演会, Sep. 2003, Japanese, 佐賀大学農学部, Domestic conference浮稲茎葉部の重力屈性反応についてOral presentation
■ Research Themes
- 日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2025 - 31 Mar. 2028低酸素が誘導するイネ伸長成長のメカニズムとその制御による新規洪水耐性付与の可能性
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Kobe University, Apr. 2022 - Mar. 2026, Principal investigatorAdaptive responses to flooding in wild rice species with the CCDD genome
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Kobe University, Apr. 2021 - Mar. 2025, Coinvestigator高温前処理によるイネメソコチル伸長の制御メカニズムの解明と栽培への応用高温前処理によるメソコチル伸長誘導とジベレリン (GA) の関連について調査を行った。高温前処理によるメソコチル伸長誘導が見られない矮性品種 (短銀坊主) にGAを投与してもメソコチル伸長は見られないが,高温前処理後にGAを投与するとメソコチル伸長誘導がみられたことから,高温前処理によるメソコチル伸長誘導には一定量のGAが不可欠であると推察した。しかし,非矮性品種にGA生合成阻害剤パクロプトラゾールを処理することで,高温前処理しなかった植物体ではメソコチル伸長が有意に抑制されたのに対して,高温前処理を行った植物体ではメソコチル伸長の抑制はわずかであった。GA関連遺伝子の発現解析したところ,SD1,OsGA3ox2,OsGA2ox3 のGA生合成遺伝子は高温前処理により発現が増大し,GA不活性化関連遺伝子の中には高温前処理区で発現量が低下するものがあった。これらの結果より,高温前処理によるメソコチル伸長誘導には一定量のGAが不可欠であるが,GAの関与のみでは説明できず他の因子が関与する可能性が考えられた。 そこでエチレンとジャスモン酸の関与について調べた。エチレン反応阻害剤で処理することにより,高温前処理によるメソコチル伸長誘導効果の減少が見られた。また,ジャスモン酸生合成関連遺伝子の GAOGAO1 の発現は,日本型品種のメソコチルが短い品種で高発現していたが,高温前処理により発現が低下することがわかった。 高温前処理の実用性について調査した。高温前処理した種子を30℃と4℃で保存したところ少なくとも4ヶ月間はメソコチルの伸長誘導効果は持続し,保存温度による違いは見られなかった。高温前処理は畑状態での深播きに有効であることは以前に示したが,湛水直播においても品種によっては有効であることがわかった。
- 飯島藤十郎記念食品科学振興財団, 2020年度学術研究助成金, Apr. 2021 - Mar. 2022, Principal investigator耐湿性の強いコムギ品種の特定とその食品利用の適正評価
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Kobe University, Apr. 2018 - Mar. 2021, CoinvestigatorSequence analysis revealed that many of the strains of the wild rice species Oryza rufipogon, O. longistaminata, and O. meridionaris, which belong to the A-genome group of the genus Oryza, have the submergence-resistance gene SUB1A and the floating rice gene SNORKEL1 / 2 (SK1 / 2). The amino acid sequence of SUB1A possessed by these wild species had a homology of 78% or more with the sequence of O. sativa, and the amino acid homology of SK1 / 2 was 77% or more. When the response to flood was investigated using these strains, many strains showed floating ability, but few strains showed flood resistance, and only one strain of O. rufipogon showed both traits.
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Kobe University, Apr. 2017 - Mar. 2019, Principal investigatorIn one accession of O. glumaepatula (W1246), it was suggested that the factor causing internode elongation under submergence is induced not mainly by ethylene. For the purpose of further verifying this point, we prepared stem segments which include uppermost internodes from three accessions with SNORKEL gene known to control floating ability in cultivated rice species, Oryza sativa, and internode elongation by submergence or ethylene treatment was evaluated. As a result, ethylene treatment did not induce internode elongation as submergence in any accessions. Therefore, the result suggests that ethylene does not play a central role in internode elongation in O. glumaepatula.
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Kobe University, Apr. 2012 - Mar. 2015, CoinvestigatorWhen subjected to gradual submergence, adult plants of the Amazonian wild rice species Oryza grandiglumis accessions showed enhanced internodal elongation with rising water level and their growth response closely resembled that of deepwater varieties. On the other hand, when subjected to complete submergence, seedlings of the accessions displayed reduced shoot growth and resumed normal growth after desubmergence, similar to the response of submergence-tolerant varieties of. Neither SUBMERGENCE1A (SUB1A) nor the SNORKEL (SK) genes were detected in the accessions. These results indicate that the O. grandiglumis accessions are capable of adapting successfully to flooding by activating two contrasting mechanisms and that each mechanism of adaptation to flooding is not mediated by SUB1A or the SK genes. The enhanced internodal elongation of submerged O. grandiglumis plants is not triggered by ethylene accumulated during submergence but by the moist surroundings provided by submergence.
- Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Grant-in-Aid for Challenging Exploratory Research, Kobe University, Apr. 2013 - Mar. 2014, CoinvestigatorInternode elongation of Sagittaria trifolia plants was induced by submergence irrespective of cover soil or glass beads.On the other hand, submergence-induced internode elongation of S. pygmaea plants was dependent on the presence of cover soil or glass beads. We examined gaseous factors responsible for the enhanced internode elongation in these species, because gas accumulation easily occurs in submerged plant tissues. Treatment of the plant hormone ethylene, which is known to enhance elongation growth in many aquatic plant species, indeed stimulated leaf elongation but failed to promote internode elongation in both Sagittaria species. We found that hypoxia or anoxia generated by submergence serves as an initial growth-promoting factor for internode elongation in S. trifolia, and carbon dioxide accumulated during submergence trigger internode elongation in S. pygmaea.
