Research activity information

• Fabrication, Evolution, and Mutual Conversion of d-Fucose-Activatable and -Repressible Acetyltransferase upon Mutations

  Yuki Yanai, Miyu Tsukada, Yuki Kimura, Daisuke Umeno

  Jun. 2025, ACS Synthetic Biology

  Scientific journal


• Enhancement of Sensitivity in Aggregation-Based Whole-Cell Arsenite Sensor Utilizing Arsenic Metabolism Regulation

  Shiryu Abe, Rina Ayuba, Kyohei Ouchi, Yu-ki Tanaka, Ai Fujimoto, Akira Kitamura, Yasumitsu Ogra, Yuki Kimura, Daisuke Umeno, Shigeko Kawai-Noma

  Apr. 2025, ACS Omega

  Scientific journal


• Directed evolution of highly sensitive and stringent choline-induced gene expression controllers

  Yuki Yanai, Takayuki Hoshino, Yuki Kimura, Shigeko Kawai-Noma, Daisuke Umeno

  Nov. 2024, The Journal of General and Applied Microbiology, English

  Scientific journal


• MetJ-Based Mutually Interfering SAM-ON/SAM-OFF Biosensors

  Taro Watanabe, Yuki Kimura, Daisuke Umeno

  Feb. 2024, ACS Synthetic Biology

  Scientific journal


• Systematic promoter design for plasmid-encoded S-adenosylmethionine sensing systems

  Taro Watanabe, Yuki Kimura, Daisuke Umeno

  2024, The Journal of General and Applied Microbiology, English, Domestic magazine

  Scientific journal


• Mutational destabilisation accelerates the evolution of novel sensory and network functions

  Yuki Kimura, Shigeko Kawai-Noma, Daisuke Umeno

  Nov. 2023


• Transcription Factors as Evolvable Biosensors

  Daisuke UMENO, Yuki KIMURA, Shigeko KAWAI-NOMA

  Springer Science and Business Media {LLC}, May 2021, Analytical Sciences, 37(5) (5), 699 - 705, English

  Scientific journal


• Laborless, automated microfluidic tandem cell processor for visualizing intracellular molecules of mammalian cells

  Mu, T., Toyoda, H., Kimura, Y., Yamada, M., Utoh, R., Umeno, D., Seki, M.

  2020, Analytical Chemistry, 92(3) (3)

  Scientific journal


• Directed evolution of transcriptional switches using dual-selector systems

  Kimura, Y., Umeno, D.

  2020, Methods in Enzymology

  Scientific journal


• Directed Evolution of the Stringency of the LuxR Vibrio fischeri Quorum Sensor without OFF-State Selection

  Kimura, Y., Kawai-Noma, S., Saito, K., Umeno, D.

  2020, ACS Synthetic Biology, 9(3) (3)

  Scientific journal


• Tweezing the cofactor preference of gymnosperm pinene synthase

  Tashiro, M., Ono, K., Kimura, Y., Kawai-Noma, S., Saito, K., Umeno, D.

  2018, Bioscience, Biotechnology and Biochemistry, 82(6) (6)

  Scientific journal


• Directed evolution of the autoinducer selectivity of Vibrio fischeri LuxR

  Tashiro, Y., Kimura, Y., Furubayashi, M., Tanaka, A., Terakubo, K., Saito, K., Kawai-Noma, S., Umeno, D.

  2016, Journal of General and Applied Microbiology, 62(5) (5)

  Scientific journal


• Directed evolution of Vibrio fischeri LuxR signal sensitivity

  Kimura, Y., Tashiro, Y., Saito, K., Kawai-Noma, S., Umeno, D.

  2016, Journal of Bioscience and Bioengineering, 122(5) (5)

  Scientific journal

• 検出素子としての転写因子と遺伝子回路構築による応答調節

  木村 友紀

  Lead, May 2026, ぶんせき, 5, 155 - 156


• 大腸菌におけるSAMバイオセンシングシステム

  渡邊 太郎, 木村 友紀, 梅野 太輔

  Sep. 2024, バイオサイエンスとインダストリー, 82(5) (5), 497 - 499


• 安定性変化を利用したタンパク質機能の集積化

  木村 友紀

  Lead, Mar. 2020, 千葉大学学位論文

• スマートセルインダストリー : 微生物細胞を用いた物質生産の展望

  木村 友紀, 関 貴洋, 大谷 悠介, 栗原 健人, 梅野 太輔

  Contributor, バイオセンサーを利用したハイスループット評価技術, 2, 44–50, シーエムシー出版, Jun. 2018, Japanese, ISBN: 9784781313344