Research activity information

• Preclinical evaluation of the efficacy of an antibody to human SIRPα for cancer immunotherapy in humanized mouse models

  Yasuyuki Saito, Rie Iida-Norita, Tania Afroj, Alaa Refaat, Daisuke Hazama, Satomi Komori, Shinya Ohata, Tomoko Takai, Okechi S. Oduori, Takenori Kotani, Yohei Funakoshi, Yu-Ichiro Koma, Yoji Murata, Kimikazu Yakushijin, Hiroshi Matsuoka, Hironobu Minami, Hiroshi Yokozaki, Markus G. Manz, Takashi Matozaki

  Tumor-associated macrophages (TAMs) are abundant in the tumor microenvironment and are considered potential targets for cancer immunotherapy. To examine the antitumor effects of agents targeting human TAMs in vivo, we here established preclinical tumor xenograft models based on immunodeficient mice that express multiple human cytokines and have been reconstituted with a human immune system by transplantation of human CD34⁺ hematopoietic stem and progenitor cells (HIS-MITRG mice). HIS-MITRG mice supported the growth of both human cell line (Raji)– and patient-derived B cell lymphoma as well as the infiltration of human macrophages into their tumors. We examined the potential antitumor action of an antibody to human SIRPα (SE12C3) that inhibits the interaction of CD47 on tumor cells with SIRPα on human macrophages and thereby promotes Fcγ receptor–mediated phagocytosis of the former cells by the latter. Treatment with the combination of rituximab (antibody to human CD20) and SE12C3 inhibited Raji tumor growth in HIS-MITRG mice to a markedly greater extent than did rituximab monotherapy. This enhanced antitumor effect was dependent on human macrophages and attributable to enhanced rituximab-dependent phagocytosis of lymphoma cells by human macrophages. Treatment with rituximab and SE12C3 also induced reprogramming of human TAMs toward a proinflammatory phenotype. Furthermore, the combination treatment essentially prevented the growth of patient-derived diffuse large B cell lymphoma in HIS-MITRG mice. Our findings thus support the study of HIS-MITRG mice as a model for the preclinical evaluation in vivo of potential therapeutics, such as antibodies to human SIRPα, that target human TAMs.

  Frontiers Media SA, Dec. 2023, Frontiers in Immunology, 14

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• CD47 promotes peripheral T cell survival by preventing dendritic cell–mediated T cell necroptosis

  Satomi Komori, Yasuyuki Saito, Taichi Nishimura, Datu Respatika, Hiromi Endoh, Hiroki Yoshida, Risa Sugihara, Rie Iida-Norita, Tania Afroj, Tomoko Takai, Okechi S. Oduori, Eriko Nitta, Takenori Kotani, Yoji Murata, Yoriaki Kaneko, Ryo Nitta, Hiroshi Ohnishi, Takashi Matozaki

  Conventional dendritic cells (cDCs) are required for peripheral T cell homeostasis in lymphoid organs, but the molecular mechanism underlying this requirement has remained unclear. We here show that T cell–specific CD47-deficient ( Cd47^ΔT ) mice have a markedly reduced number of T cells in peripheral tissues. Direct interaction of CD47-deficient T cells with cDCs resulted in activation of the latter cells, which in turn induced necroptosis of the former cells. The deficiency and cell death of T cells in Cd47^ΔT mice required expression of its receptor signal regulatory protein α on cDCs. The development of CD4 ⁺ T helper cell–dependent contact hypersensitivity and inhibition of tumor growth by cytotoxic CD8 ⁺ T cells were both markedly impaired in Cd47^ΔT mice. CD47 on T cells thus likely prevents their necroptotic cell death initiated by cDCs and thereby promotes T cell survival and function.

  Proceedings of the National Academy of Sciences, Aug. 2023, Proceedings of the National Academy of Sciences, 120(33) (33)

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 【新しいがん免疫療法研究の展開と臨床応用】自然免疫制御を介したがん免疫療法の進展 CD47-SIRPα経路の制御によるがん免疫療法

  小谷 武徳, 村田 陽二, 齊藤 泰之, 的崎 尚

  (有)科学評論社, Mar. 2023, 腫瘍内科, 31(3) (3), 327 - 333, Japanese


• Targeting of SIRPα as a potential therapy for Langerhans cell histiocytosis.

  Okamoto T, Murata Y, Hasegawa D, Yoshida M, Tanaka D, Ueda T, Hazama D, Oduori OS, Komori S, Takai T, Saito Y, Kotani T, Kosaka Y, Maniwa Y, Matozaki T

  Feb. 2023, Cancer science

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The Role of Type-2 Conventional Dendritic Cells in the Regulation of Tumor Immunity.

  Yasuyuki Saito, Satomi Komori, Takenori Kotani, Yoji Murata, Takashi Matozaki

  Conventional dendritic cells (cDCs) orchestrate immune responses to cancer and comprise two major subsets: type-1 cDCs (cDC1s) and type-2 cDCs (cDC2s). Compared with cDC1s, which are dedicated to the activation of CD8+ T cells, cDC2s are ontogenically and functionally heterogeneous, with their main function being the presentation of exogenous antigens to CD4+ T cells for the initiation of T helper cell differentiation. cDC1s play an important role in tumor-specific immune responses through cross-presentation of tumor-derived antigens for the priming of CD8+ T cells, whereas little is known of the role of cDC2s in tumor immunity. Recent studies have indicated that human cDC2s can be divided into at least two subsets and have implicated these cells in both anti- and pro-tumoral immune responses. Furthermore, the efficacy of cDC2-based vaccines as well as cDC2-targeted therapeutics has been demonstrated in both mouse models and human patients. Here we summarize current knowledge about the role of cDC2s in tumor immunity and address whether these cells are beneficial in the context of antitumor immune responses.

  Apr. 2022, Cancers, 14(8) (8), English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Anticancer efficacy of monotherapy with antibodies to SIRPα/SIRPβ1 mediated by induction of antitumorigenic macrophages

  Mariko Sakamoto, Yoji Murata, Daisuke Tanaka, Yuka Kakuchi, Takeshi Okamoto, Daisuke Hazama, Yasuyuki Saito, Takenori Kotani, Hiroshi Ohnishi, Masayuki Miyasaka, Masato Fujisawa, Takashi Matozaki

  The interaction of signal regulatory protein α (SIRPα) on macrophages with CD47 on cancer cells is thought to prevent antibody (Ab)-dependent cellular phagocytosis (ADCP) of the latter cells by the former. Blockade of the CD47-SIRPα interaction by Abs to CD47 or to SIRPα, in combination with tumor-targeting Abs such as rituximab, thus inhibits tumor formation by promoting macrophage-mediated ADCP of cancer cells. Here we show that monotherapy with a monoclonal Ab (mAb) to SIRPα that also recognizes SIRPβ1 inhibited tumor formation by bladder and mammary cancer cells in mice, with this inhibitory effect being largely dependent on macrophages. The mAb to SIRPα promoted polarization of tumor-infiltrating macrophages toward an antitumorigenic phenotype, resulting in the killing and phagocytosis of cancer cells by the macrophages. Ablation of SIRPα in mice did not prevent the inhibitory effect of the anti-SIRPα mAb on tumor formation or its promotion of the cancer cell–killing activity of macrophages, however. Moreover, knockdown of SIRPβ1 in macrophages attenuated the stimulatory effect of the anti-SIRPα mAb on the killing of cancer cells, whereas an mAb specific for SIRPβ1 mimicked the effect of the anti-SIRPα mAb. Our results thus suggest that monotherapy with Abs to SIRPα/SIRPβ1 induces antitumorigenic macrophages and thereby inhibits tumor growth and that SIRPβ1 is a potential target for cancer immunotherapy.

  Proceedings of the National Academy of Sciences, Jan. 2022, Proceedings of the National Academy of Sciences, 119(1) (1), e2109923118 - e2109923118

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 免疫系ヒト化マウスモデルによるヒトマクロファージを標的としたがん免疫療法の開発

  齊藤 泰之, 飯田 理恵, 羽間 大祐, 小谷 武徳, 村田 陽二, 横崎 宏, 的崎 尚

  (一社)日本癌学会, Sep. 2021, 日本癌学会総会記事, 80回, [E2 - 6], English


• Role of Ras in regulation of intestinal epithelial cell homeostasis and crosstalk with Wnt signaling.

  Takenori Kotani, Noriko Ihara, Saki Okamoto, Jajar Setiawan, Tasuku Konno, Yasuyuki Saito, Yoji Murata, Takashi Matozaki

  Cross talk between different signaling pathways is thought to be important for regulation of homeostasis of, as well as oncogenesis of, the intestinal epithelium. Expression of an active form of K-Ras specifically in intestinal epithelial cells (IECs) of mice (IEC-RasDA mice) resulted in the development of hyperplasia in the small intestine and colon of mice. IEC-RasDA mice also manifested the increased proliferation of IECs. In addition, the number of goblet cells markedly increased, while that of Paneth cells decreased in IEC-RasDA mice. Development of intestinal organoids was markedly enhanced for IEC-RasDA mice compared with control mice. Whereas, the expression of Wnt target genes was significantly reduced in the in intestinal crypts from IEC-RasDA mice compared with that apparent for the control. Our results thus suggest that K-Ras promotes the proliferation of IECs as well as generation of goblet cells. By contrast, Ras counter-regulates the Wnt signaling and thereby contribute to the proper regulation of intestinal epithelial cell homeostasis.

  2021, PloS one, 16(8) (8), e0256774, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Roles of Src family kinase, Ras, and mTOR signaling in intestinal epithelial homeostasis and tumorigenesis.

  Takashi Matozaki, Takenori Kotani, Yoji Murata, Yasuyuki Saito

  The turnover of intestinal epithelial cells (IECs) is relatively rapid (3-5 days in mouse and human), and this short existence and other aspects of the homeostasis of IECs are tightly regulated by various signaling pathways including Wnt-β-catenin signaling. Dysregulation of IEC homeostasis likely contributes to the development of intestinal inflammation and intestinal cancer. The roles of receptor protein tyrosine kinases and their downstream signaling molecules such as Src family kinases, Ras, and mTOR in homeostatic regulation of IEC turnover have recently been evaluated. These signaling pathways have been found to promote not only the proliferation of IECs but also the differentiation of progenitor cells into secretory cell types such as goblet cells. Of note, signaling by Src family kinases, Ras, and mTOR has been shown to oppose the Wnt-β-catenin signaling pathway and thereby to limit the number of Lgr5+ intestinal stem cells or of Paneth cells. Such cross-talk of signaling pathways is important not only for proper regulation of IEC homeostasis but for the development of intestinal tumors and potentially for anticancer therapy.

  Jan. 2021, Cancer science, 112(1) (1), 16 - 21, English, International magazine

  [Refereed][Invited]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 免疫系ヒト化マウスモデルによるヒトマクロファージを標的としたがん免疫療法の開発

  齋藤 泰之, 飯田 理恵, 羽間 大祐, 小谷 武徳, 村田 陽二, 的崎 尚

  (一社)日本癌学会, Oct. 2020, 日本癌学会総会記事, 79回, OE2 - 4, English


• SIRPα on CD11c+ cells induces Th17 cell differentiation and subsequent inflammation in the CNS in experimental autoimmune encephalomyelitis.

  Taichi Nishimura, Yasuyuki Saito, Ken Washio, Satomi Komori, Datu Respatika, Takenori Kotani, Yoji Murata, Hiroshi Ohnishi, Satoshi Mizobuchi, Takashi Matozaki

  Signal regulatory protein α (SIRPα) is expressed predominantly on type 2 conventional dendritic cells (cDC2s) and macrophages. We previously showed that mice systemically lacking SIRPα were resistant to experimental autoimmune encephalomyelitis (EAE). Here, we showed that deletion of SIRPα in CD11c+ cells of mice (SirpaΔDC mice) also markedly ameliorated the development of EAE. The frequency of cDCs and migratory DCs (mDCs), as well as that of Th17 cells, were significantly reduced in draining lymph nodes of SirpaΔDC mice at the onset of EAE. In addition, we found the marked reduction in the number of Th17 cells and DCs in the CNS of SirpaΔDC mice at the peak of EAE. Whereas inducible systemic ablation of SIRPα before the induction of EAE prevented disease development, that after EAE onset did not ameliorate the clinical signs of disease. We also found that EAE development was partially attenuated in mice with CD11c+ cell-specific ablation of CD47, a ligand of SIRPα. Collectively, our results suggest that SIRPα expressed on CD11c+ cells, such as cDC2s and mDCs, is indispensable for the development of EAE, being required for the priming of self-reactive Th17 cells in the periphery as well as for the inflammation in the CNS.

  Oct. 2020, European journal of immunology, 50(10) (10), 1560 - 1570, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal


• Blockade of CD47 or SIRPα: a new cancer immunotherapy.

  Yoji Murata, Yasuyuki Saito, Takenori Kotani, Takashi Matozaki

  The CD47-Signal regulatory protein α (SIRPα) singling axis acts as a crucial regulator that limits the phagocytic activity of professional phagocytes such as macrophages. Recent studies have demonstrated that the interaction between CD47 on tumor cells and SIRPα on macrophages is implicated in the ability of tumors to evade immunosurveillance. Targeting the CD47-SIRPα interaction is therefore considered to be a promising approach for cancer therapy. Herein, we review some of studies displaying the potential clinical application of antibodies and other modalities that target the CD47-SIRPα interaction. Current limitations of the CD47-SIRPα-targeted immunotherapeutic approaches are also discussed as well as other avenues for future study to improve the current strategies in targeting the CD47-SIRPα signaling axis for cancer immunotherapy.

  Oct. 2020, Expert opinion on therapeutic targets, 24(10) (10), 945 - 951, English, International magazine

  [Refereed]


• Macrocyclic Peptide-Mediated Blockade of the CD47-SIRPα Interaction as a Potential Cancer Immunotherapy.

  Daisuke Hazama, Yizhen Yin, Yoji Murata, Makoto Matsuda, Takeshi Okamoto, Daisuke Tanaka, Naohiro Terasaka, Jinxuan Zhao, Mariko Sakamoto, Yuka Kakuchi, Yasuyuki Saito, Takenori Kotani, Yoshihiro Nishimura, Atsushi Nakagawa, Hiroaki Suga, Takashi Matozaki

  Medium-sized macrocyclic peptides are an alternative to small compounds and large biomolecules as a class of pharmaceutics. The CD47-SIRPα signaling axis functions as an innate immune checkpoint that inhibits phagocytosis in phagocytes and has been implicated as a promising target for cancer immunotherapy. The potential of macrocyclic peptides that target this signaling axis as immunotherapeutic agents has remained unknown, however. Here we have developed a macrocyclic peptide consisting of 15 amino acids that binds to the ectodomain of mouse SIRPα and efficiently blocks its interaction with CD47 in an allosteric manner. The peptide markedly promoted the phagocytosis of antibody-opsonized tumor cells by macrophages in vitro as well as enhanced the inhibitory effect of anti-CD20 or anti-gp75 antibodies on tumor formation or metastasis in vivo. Our results suggest that allosteric inhibition of the CD47-SIRPα interaction by macrocyclic peptides is a potential approach to cancer immunotherapy.

  Sep. 2020, Cell chemical biology, 27(9) (9), 1181 - 1191, English, International magazine

  [Refereed]

  Scientific journal


• Regulation of colonic epithelial cell homeostasis by mTORC1.

  Takenori Kotani, Jajar Setiawan, Tasuku Konno, Noriko Ihara, Saki Okamoto, Yasuyuki Saito, Yoji Murata, Tetsuo Noda, Takashi Matozaki

  Cell signaling important for homeostatic regulation of colonic epithelial cells (CECs) remains poorly understood. Mammalian target of rapamycin complex 1 (mTORC1), a protein complex that contains the serine-threonine kinase mTOR, mediates signaling that underlies the control of cellular functions such as proliferation and autophagy by various external stimuli. We here show that ablation of tuberous sclerosis complex 2 (Tsc2), a negative regulator of mTORC1, specifically in intestinal epithelial cells of mice resulted in increased activity of mTORC1 of, as well as increased proliferative activity of, CECs. Such Tsc2 ablation also reduced the population of Lgr5-positive colonic stem cells and the expression of Wnt target genes in CECs. The stimulatory phosphorylation of the kinase Akt and inhibitory phosphorylation of glycogen synthase kinase 3β were both markedly decreased in the colon of the Tsc2 conditional knockout (CKO) mice. Development of colonic organoids with cryptlike structures was enhanced for Tsc2 CKO mice compared with control mice. Finally, Tsc2 CKO mice manifested increased susceptibility to dextran sulfate sodium-induced colitis. Our results thus suggest that mTORC1 activity promotes the proliferation of, as well as the expression of Wnt target genes in, CECs and thereby contributes to colonic organogenesis and homeostasis.

  Lead, Aug. 2020, Scientific reports, 10(1) (1), 13810 - 13810, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• SIRPα+ dendritic cells promote the development of fibroblastic reticular cells in murine peripheral lymph nodes.

  Satomi Komori, Yasuyuki Saito, Datu Respatika, Taichi Nishimura, Takenori Kotani, Yoji Murata, Takashi Matozaki

  Nonhematopoietic stromal cells contribute to the organization and homeostasis of secondary lymphoid organs by producing cytokines and chemokines. The development and maintenance of these stromal cells are thought to be regulated by innate immune cells. Indeed, we recently showed that signal regulatory protein α (SIRPα)-positive dendritic cells (DCs) are essential for the proliferation and survival of podoplanin (Pdpn)-positive fibroblastic reticular cells (FRCs) in mouse spleen. We have now established an in vitro culture system for lymph node stromal cells (LNSCs) isolated from mouse peripheral LNs. Activated DCs and TNF-α each promoted the proliferation of cultured LNSCs, most of which were found to be Pdpn+ FRCs. Furthermore, ablation of SIRPα in CD11c+ cells attenuated this effect of DCs on LNSC proliferation. Transplantation of activated DCs together with cultured LNSCs into the renal subcapsular space markedly increased the number of ER-TR7+ stromal cells as well as induced the accumulation of T cells and increased the expression of Ccl19 in the transplants. Ablation of SIRPα in CD11c+ cells greatly impaired the development of LN-like structure in the transplants. Our findings thus suggest that SIRPα+ DCs are important for the proliferation and differentiation of Pdpn+ FRCs in peripheral LNs.

  Wiley, Sep. 2019, European journal of immunology, 49(9) (9), 1364 - 1371, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Regulation of Small Intestinal Epithelial Homeostasis by Tsc2-mTORC1 Signaling.

  Jajar Setiawan, Takenori Kotani, Tasuku Konno, Yasuyuki Saito, Yoji Murata, Tetsuo Noda, Takashi Matozaki

  Mammalian target of rapamycin complex 1 (mTORC1), a protein complex containing the serine/threonine kinase mTOR, integrates various growth stimulating signals. mTORC1 is expressed in intestinal epithelial cells (IECs), whereas the physiological roles of this protein complex in homeostasis of IECs remain virtually unknown. We here generated mice, in which tuberous sclerosis complex 2 (Tsc2), a negative regulator of mTORC1, was specifically ablated in IECs (Tsc2 CKO mice). Ablation of Tsc2 enhanced the phosphorylation of mTORC1 downstream molecules such as ribosomal S6 protein and 4E-BP1 in IECs. Tsc2 CKO mice manifested the enhanced proliferative activity of IECs in intestinal crypts as well as the promoted migration of these cells along the crypt-villus axis. The mutant mice also manifested the increased apoptotic rate of IECs as well as the increased ectopic Paneth cells, which are one of the major differentiated IECs. In addition, in vitro study showed that ablation of Tsc2 promoted the development of intestinal organoids without epidermal growth factor, while mTORC1 inhibitor, rapamycin, diminished this phenotype. Our results thus suggest that Tsc2-mTORC1 signaling regulates the proliferation, migration, and positioning of IECs, and thereby contributes to the proper regulation of intestinal homeostasis.

  Apr. 2019, The Kobe journal of medical sciences, 64(6) (6), E200-E209 - E209, English, Domestic magazine, Co-authored internationally

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Microglial SIRPα regulates the emergence of CD11c+ microglia and demyelination damage in white matter.

  Miho Sato-Hashimoto, Tomomi Nozu, Riho Toriba, Ayano Horikoshi, Miho Akaike, Kyoko Kawamoto, Ayaka Hirose, Yuriko Hayashi, Hiromi Nagai, Wakana Shimizu, Ayaka Saiki, Tatsuya Ishikawa, Ruwaida Elhanbly, Takenori Kotani, Yoji Murata, Yasuyuki Saito, Masae Naruse, Koji Shibasaki, Per-Arne Oldenborg, Steffen Jung, Takashi Matozaki, Yugo Fukazawa, Hiroshi Ohnishi

  A characteristic subset of microglia expressing CD11c appears in response to brain damage. However, the functional role of CD11c+ microglia, as well as the mechanism of its induction, are poorly understood. Here we report that the genetic ablation of signal regulatory protein α (SIRPα), a membrane protein, induced the emergence of CD11c+ microglia in the brain white matter. Mice lacking CD47, a physiological ligand of SIRPα, and microglia-specific SIRPα-knockout mice exhibited the same phenotype, suggesting that an interaction between microglial SIRPα and CD47 on neighbouring cells suppressed the emergence of CD11c+ microglia. A lack of SIRPα did not cause detectable damage to the white matter, but resulted in the increased expression of genes whose expression is characteristic of the repair phase after demyelination. In addition, cuprizone-induced demyelination was alleviated by the microglia-specific ablation of SIRPα. Thus, microglial SIRPα suppresses the induction of CD11c+ microglia that have the potential to accelerate the repair of damaged white matter.

  Mar. 2019, eLife, 8(. pii) (. pii), e42025, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Role of lysophosphatidic acid in proliferation and differentiation of intestinal epithelial cells.

  Tasuku Konno, Takenori Kotani, Jajar Setiawan, Yuka Nishigaito, Naoki Sawada, Shinya Imada, Yasuyuki Saito, Yoji Murata, Takashi Matozaki

  Intestinal epithelial cells (IECs) are regenerated continuously from intestinal stem cells (ISCs) near the base of intestinal crypts in order to maintain homeostasis and structural integrity of intestinal epithelium. Epidermal growth factor (EGF) is thought to be important to drive the proliferation and differentiation of IECs from ISCs, it remains unknown whether other growth factors or lipid mediators are also important for such regulation, however. Here we show that lysophosphatidic acid (LPA), instead of EGF, robustly promoted the development of intestinal organoids prepared from the mouse small intestine. Indeed, LPA exhibited the proliferative activity of IECs as well as induction of differentiation of IECs into goblet cells, Paneth cells, and enteroendocrine cells in intestinal organoids. Inhibitors for LPA receptor 1 markedly suppressed the LPA-promoted development of intestinal organoids. LPA also promoted the phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 in intestinal organoids, whereas inhibition of mitogen-activated protein kinase/ERK kinase (MEK) 1/2 significantly suppressed the development of, as well as the proliferative activity and differentiation of, intestinal organoids in response to LPA. Our results thus suggest that LPA is a key factor that drives the proliferation and differentiation of IECs.

  Corresponding, 2019, PloS one, 14(4) (4), e0215255, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Role of Csk in intestinal epithelial barrier function and protection against colitis.

  Chunxiao Sun, Yoji Murata, Shinya Imada, Tasuku Konno, Takenori Kotani, Yasuyuki Saito, Hideto Yamada, Takashi Matozaki

  Intestinal epithelial cells (IECs) play a pivotal role in the maintenance of the integrity and barrier function of the intestinal epithelium. Dysfunctions of IECs are thought to participate in the disruption of the intestinal epithelial barrier, resulting in gastrointestinal diseases, such as colitis and colorectal cancer. Here we show that IEC-specific COOH-terminal Src kinase (Csk)-deficient mice (Csk CKO mice) manifested the increased susceptibility to dextran sodium sulfate (DSS)-induced colitis, a model of inflammatory bowel disease. DSS-treated Csk CKO mice also exhibited the significantly elevated intestinal permeability. Following DSS treatment, Csk CKO mice exhibited the higher proliferative activity of colonic epithelial cells and the increased number of apoptotic cells in the colon compared with that apparent for control mice. Moreover, the abundance of the tight junction protein occludin, which regulates cell-cell adhesion as well as epithelial permeability, was markedly reduced in the colon of DSS-treated Csk CKO mice. These results thus suggest that Csk in IECs plays important roles in the regulation of the intestinal epithelial barrier function and protection against colitis.

  Sep. 2018, Biochemical and biophysical research communications, 504(1) (1), 109 - 114, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Anti-human SIRPα antibody is a new tool for cancer immunotherapy.

  Yoji Murata, Daisuke Tanaka, Daisuke Hazama, Tadahiko Yanagita, Yasuyuki Saito, Takenori Kotani, Per-Arne Oldenborg, Takashi Matozaki

  Blackwell Publishing Ltd, May 2018, Cancer science, 109(5) (5), 1300 - 1308, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Regulation by commensal bacteria of neurogenesis in the subventricular zone of adult mouse brain.

  Naoki Sawada, Takenori Kotani, Tasuku Konno, Jajar Setiawan, Yuka Nishigaito, Yasuyuki Saito, Yoji Murata, Ken-Ichi Nibu, Takashi Matozaki

  In the mouse olfactory bulb (OB), interneurons such as granule cells and periglomerular cells are continuously replaced by adult-born neurons, which are generated in the subventricular zone (SVZ) of the brain. We have now investigated the role of commensal bacteria in regulation of such neuronal cell turnover in the adult mouse brain. Administration of mixture of antibiotics to specific pathogen-free (SPF) mice markedly attenuated the incorporation of bromodeoxyuridine (BrdU) into the SVZ cells. The treatment with antibiotics also reduced newly generated BrdU-positive neurons in the mouse OB. In addition, the incorporation of BrdU into the SVZ cells of germ-free (GF) mice was markedly reduced compared to that apparent for SPF mice. In contrast, the reduced incorporation of BrdU into the SVZ cells of GF mice was recovered by their co-housing with SPF mice, suggesting that commensal bacteria promote the incorporation of BrdU into the SVZ cells. Finally, we found that administration of ampicillin markedly attenuated the incorporation of BrdU into the SVZ cells of SPF mice. Our results thus suggest that ampicillin-sensitive commensal bacteria regulate the neurogenesis in the SVZ of adult mouse brain.

  Corresponding, Apr. 2018, Biochemical and biophysical research communications, 498(4) (4), 824 - 829, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• SIRPα+ dendritic cells regulate homeostasis of fibroblastic reticular cells via TNF receptor ligands in the adult spleen.

  Yasuyuki Saito, Datu Respatika, Satomi Komori, Ken Washio, Taichi Nishimura, Takenori Kotani, Yoji Murata, Hideki Okazawa, Hiroshi Ohnishi, Yoriaki Kaneko, Katsuyuki Yui, Koji Yasutomo, Chikako Nishigori, Yoshihisa Nojima, Takashi Matozaki

  Nov. 2017, Proceedings of the National Academy of Sciences of the United States of America, 114(47) (47), E10151-E10160 - E10160, English, International magazine

  [Refereed]

  Scientific journal


• Role of SIRPα in Homeostatic Regulation of T Cells and Fibroblastic Reticular Cells in the Spleen.

  Datu Respatika, Yasuyuki Saito, Ken Washio, Satomi Komori, Takenori Kotani, Hideki Okazawa, Yoji Murata, Takashi Matozaki

  Signal regulatory protein α (SIRPα), is an immunoglobulin superfamily protein that is predominantly expressed in macrophages and dendritic cells (DCs), especially CD4+ conventional DCs (cDCs). In this study, we demonstrated that, in addition to the reduced number of CD4+ cDCs, the number of T cells was significantly decreased in the spleen of Sirpa-/- mice, in which full-length of SIRPα protein was systemically ablated. The size of the T cell zone was markedly reduced in the spleen of Sirpa-/- mice. In addition, Sirpa-/- mice revealed a marked reduction of CCL19, CCL21, and IL-7 expression, which are thought to be important for homeostasis of T cells in the spleen. Consistently, the abundance of fibroblastic reticular cells (FRCs), a subset of stromal cells in the T cell zone, was markedly reduced in the spleen of Sirpa-/- mice compared with Sirpaf/f mice. Moreover, we demonstrated that the mRNA expression of Lymphotoxin (LT) α, LTβ, and LIGHT was significantly reduced in the spleen of Sirpa-/- mice. These data thus suggest that SIRPα is essential for steady-state homeostasis of T cells and FRCs in the spleen.

  Aug. 2017, The Kobe journal of medical sciences, 63(1) (1), E22-E29 - E29, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Role of SIRPα in Homeostatic Regulation of T Cells and Fibroblastic Reticular Cells in the Spleen

  Respatika Datu, Saito Yasuyuki, Washio Ken, Komori Satomi, Kotani Takenori, Okazawa Hideki, Murata Yoji, Matozaki Takashi

  Signal regulatory protein α (SIRPα), is an immunoglobulin superfamily protein that is predominantly expressed in macrophages and dendritic cells (DCs), especially CD4+ conventional DCs (cDCs). In this study, we demonstrated that, in addition to the reduced number of CD4+ cDCs, the number of T cells was significantly decreased in the spleen of Sirpa–/– mice, in which full-length

  神戸大学医学部, Aug. 2017, The Kobe journal of the medical sciences, 63(1) (1), 22 - 29, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Anti-SIRPα antibodies as a potential new tool for cancer immunotherapy.

  Tadahiko Yanagita, Yoji Murata, Daisuke Tanaka, Sei-Ichiro Motegi, Eri Arai, Edwin Widyanto Daniwijaya, Daisuke Hazama, Ken Washio, Yasuyuki Saito, Takenori Kotani, Hiroshi Ohnishi, Per-Arne Oldenborg, Noel Verjan Garcia, Masayuki Miyasaka, Osamu Ishikawa, Yae Kanai, Takahide Komori, Takashi Matozaki

  Tumor cells are thought to evade immune surveillance through interaction with immune cells. Much recent attention has focused on the modification of immune responses as a basis for new cancer treatments. SIRPα is an Ig superfamily protein that inhibits phagocytosis in macrophages upon interaction with its ligand CD47 expressed on the surface of target cells. Here, we show that SIRPα is highly expressed in human renal cell carcinoma and melanoma. Furthermore, an anti-SIRPα Ab that blocks the interaction with CD47 markedly suppressed tumor formation by renal cell carcinoma or melanoma cells in immunocompetent syngeneic mice. This inhibitory effect of the Ab appeared to be mediated by dual mechanisms: direct induction of Ab-dependent cellular phagocytosis of tumor cells by macrophages and blockade of CD47-SIRPα signaling that negatively regulates such phagocytosis. The antitumor effect of the Ab was greatly attenuated by selective depletion not only of macrophages but also of NK cells or CD8+ T cells. In addition, the anti-SIRPα Ab also enhances the inhibitory effects of Abs against CD20 and programmed cell death 1 (PD-1) on tumor formation in mice injected with SIRPα-nonexpressing tumor cells. Anti-SIRPα Abs thus warrant further study as a potential new therapy for a broad range of cancers.

  Jan. 2017, JCI insight, 2(1) (1), e89140, English, International magazine, Co-authored internationally

  [Refereed]

  Scientific journal


• Role of Src Family Kinases in Regulation of Intestinal Epithelial Homeostasis.

  Shinya Imada, Yoji Murata, Takenori Kotani, Masaki Hatano, Chunxiao Sun, Tasuku Konno, Jung-Ha Park, Yasuaki Kitamura, Yasuyuki Saito, Hideki Ohdan, Takashi Matozaki

  Nov. 2016, Molecular and cellular biology, 36(22) (22), 2811 - 2823, English, International magazine

  [Refereed]

  Scientific journal


• Promotion of Intestinal Epithelial Cell Turnover by Commensal Bacteria: Role of Short-Chain Fatty Acids.

  Jung-Ha Park, Takenori Kotani, Tasuku Konno, Jajar Setiawan, Yasuaki Kitamura, Shinya Imada, Yutaro Usui, Naoya Hatano, Masakazu Shinohara, Yasuyuki Saito, Yoji Murata, Takashi Matozaki

  Corresponding, 2016, PloS one, 11(5) (5), e0156334, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Protein tyrosine phosphatase SAP-1 protects against colitis through regulation of CEACAM20 in the intestinal epithelium

  Yoji Murata, Takenori Kotani, Yana Supriatna, Yasuaki Kitamura, Shinya Imada, Kohichi Kawahara, Miki Nishio, Edwin Widyanto Daniwijaya, Hisanobu Sadakata, Shinya Kusakari, Munemasa Mori, Yoshitake Kanazawa, Yasuyuki Saito, Katsuya Okawa, Mariko Takeda-Morishita, Hideki Okazawa, Hiroshi Ohnishi, Takeshi Azuma, Akira Suzuki, Takashi Matozaki

  Lead, Aug. 2015, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 112(31) (31), E4264 - E4271, English, International magazine

  [Refereed]

  Scientific journal


• Regulation by gut commensal bacteria of carcinoembryonic antigen-related cell adhesion molecule expression in the intestinal epithelium

  Yasuaki Kitamura, Yoji Murata, Jung-ha Park, Takenori Kotani, Shinya Imada, Yasuyuki Saito, Hideki Okazawa, Takeshi Azuma, Takashi Matozaki

  Jul. 2015, GENES TO CELLS, 20(7) (7), 578 - 589, English

  [Refereed]

  Scientific journal


• Dendritic cell SIRP regulates homeostasis of dendritic cells in lymphoid organs

  Ken Washio, Takenori Kotani, Yasuyuki Saito, Datu Respatika, Yoji Murata, Yoriaki Kaneko, Hideki Okazawa, Hiroshi Ohnishi, Atsushi Fukunaga, Chikako Nishigori, Takashi Matozaki

  Jun. 2015, GENES TO CELLS, 20(6) (6), 451 - 463, English

  [Refereed]

  Scientific journal


• Dendritic cell SIRPα regulates homeostasis of dendritic cells in lymphoid organs

  Ken Washio, Takenori Kotani, Yasuyuki Saito, Datu Respatika, Yoji Murata, Yoriaki Kaneko, Hideki Okazawa, Hiroshi Ohnishi, Atsushi Fukunaga, Chikako Nishigori, Takashi Matozaki

  Blackwell Publishing Ltd, Jun. 2015, Genes to Cells, 20(6) (6), 451 - 463, English

  [Refereed]

  Scientific journal


• Shp2 in forebrain neurons regulates synaptic plasticity, locomotion, and memory formation in mice

  Kusakari S, Saitow F, Ago Y, Shibasaki K, Sato-Hashimoto M, Matsuzaki Y, Kotani Takenori, Murata Yoji, Hirai H, Matsuda T, Suzuki H, Matozaki Takashi, Ohnishi H

  Shp2 (Src homology 2 domain-containing protein tyrosine phosphatase 2) regulates neural cell differentiation. It is also expressed in postmitotic neurons, however, and mutations of Shp2 are associated with clinical syndromes characterized by mental retardation. Here we show that conditional-knockout (cKO) mice lacking Shp2 specifically in postmitotic forebrain neurons manifest abnormal behavior, including hyperactivity. Novelty-induced expression of immediate-early genes and activation of extracellular-signal-regulated kinase (Erk) were attenuated in the cerebral cortex and hippocampus of Shp2 cKO mice, suggestive of reduced neuronal activity. In contrast, ablation of Shp2 enhanced high-K(+)-induced Erk activation in both cultured cortical neurons and synaptosomes, whereas it inhibited that induced by brain-derived growth factor in cultured neurons. Posttetanic potentiation and paired-pulse facilitation were attenuated and enhanced, respectively, in hippocampal slices from Shp2 cKO mice. The mutant mice also manifested transient impairment of memory formation in the Morris water maze. Our data suggest that Shp2 contributes to regulation of Erk activation and synaptic plasticity in postmitotic forebrain neurons and thereby controls locomotor activity and memory formation.

  Feb. 2015, Mol Cell Biol, 35(9) (9), 1557 - 72, English, International magazine

  [Refereed]

  Scientific journal


• The CD47-SIRP alpha signalling system: its physiological roles and therapeutic application

  Yoji Murata, Takenori Kotani, Hiroshi Ohnishi, Takashi Matozaki

  Jun. 2014, JOURNAL OF BIOCHEMISTRY, 155(6) (6), 335 - 344, English

  [Refereed]

  Scientific journal


• Shear Stress-induced Redistribution of Vascular Endothelial-Protein-tyrosine Phosphatase (VE-PTP) in Endothelial Cells and Its Role in Cell Elongation

  Kemala Isnainiasih Mantilidewi, Yoji Murata, Munemasa Mori, Chihiro Otsubo, Takenori Kotani, Shinya Kusakari, Hiroshi Ohnishi, Takashi Matozaki

  Mar. 2014, JOURNAL OF BIOLOGICAL CHEMISTRY, 289(10) (10), 6451 - 6461, English

  [Refereed]

  Scientific journal


• Role of the Protein Tyrosine Phosphatase Shp2 in Homeostasis of the Intestinal Epithelium

  Hironori Yamashita, Takenori Kotani, Jung-ha Park, Yoji Murata, Hideki Okazawa, Hiroshi Ohnishi, Yonson Ku, Takashi Matozaki

  Corresponding, Mar. 2014, PLOS ONE, 9(3) (3), e92904, English

  [Refereed]

  Scientific journal


• Autoimmune animal models in the analysis of the CD47-SIRP alpha signaling pathway

  Yoji Murata, Yasuyuki Saito, Tetsuya Kaneko, Takenori Kotani, Yoriaki Kaneko, Hiroshi Ohnishi, Takashi Matozaki

  Jan. 2014, METHODS, 65(2) (2), 254 - 259, English

  [Refereed]

  Scientific journal


• Tyrosine Phosphorylation of Carcinoembryonic Antigen-related Cell Adhesion Molecule 20 and Its Functional Role.

  Edwin Widyanto Daniwijaya, Yoji Murata, Takenori Kotani, Yasuaki Kitamura, Kemala Isnainiasih Mantilidewi, Shinya Kusakari, Hiroshi Ohnishi, Hideki Okazawa, Takashi Matozaki

  Carcinoembryonic antigen-related cell adhesion molecule (CEACAM) 20 is an immunoglobulin-superfamily transmembrane protein that contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic region. However, the mechanism for tyrosine phosphorylation of, or the physiological function of, this protein remains largely unknown. Here we have shown that CEACAM20 is indeed tyrosine-phosphorylated by either treatment with pervanadate or forced expression of c-Src. In addition, Tyr522, Tyr559 or Tyr570, the latter two of which are within the ITAM, is likely important for such tyrosine phosphorylation. Forced expression of Myc-tagged wild-type CEACAM20 promoted the phagocytic activity of cultured cells for microbeads coupled with anti-Myc antibodies. By contrast, such phagocytic activity was markedly reduced when a mutant form of CEACAM20, in which Tyr559 and Tyr570 were substituted with phenylalanine, was expressed. Furthermore, the CEACAM20-mediated phagocytic activity was markedly prevented by the treatment with an inhibitor for either Src family kinases (SFKs), Syk, phosphoinositide 3-kinase (PI3K) or phospholipase C-γ (PLCγ). Inhibition of actin polymerization by Cytochalasin D significantly inhibited the CEACAM20-mediated phagocytosis. These results thus suggest that tyrosine phosphorylation of CEACAM20 likely promotes phagocytic activity of the cells. The CEACAM20-mediated phagocytic activity requires the activation of SFKs, Syk, PI3K or PLCγ.

  Mar. 2013, The Kobe journal of medical sciences, 59(5) (5), E172-E183, English, Domestic magazine

  Scientific journal


• Hypothermia-dependent and -independent effects of forced swim on the phosphorylation states of signaling molecules in mouse hippocampus

  Yuriko Hayashi, Shinya Kusakari, Miho Sato-Hashimoto, Eriko Urano, Masahiro Shigeno, Tsuneo Sekijima, Takenori Kotani, Yoji Murata, Hirokazu Murakami, Takashi Matozaki, Hiroshi Ohnishi

  Nov. 2012, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 428(4) (4), 475 - 481, English

  [Refereed]

  Scientific journal


• Hypothermia-induced tyrosine phosphorylation of SIRPa in the brain

  Toshi Maruyama, Shinya Kusakari, Miho Sato-Hashimoto, Yuriko Hayashi, Takenori Kotani, Yoji Murata, Hideki Okazawa, Per-Arne Oldenborg, Shoji Kishi, Takashi Matozaki, Hiroshi Ohnishi

  Jun. 2012, JOURNAL OF NEUROCHEMISTRY, 121(6) (6), 891 - 902, English

  [Refereed]

  Scientific journal


• Dendritic Cell-Specific Ablation of the Protein Tyrosine Phosphatase Shp1 Promotes Th1 Cell Differentiation and Induces Autoimmunity

  Tetsuya Kaneko, Yasuyuki Saito, Takenori Kotani, Hideki Okazawa, Hiroko Iwamura, Miho Sato-Hashimoto, Yoshitake Kanazawa, Satoshi Takahashi, Keiju Hiromura, Shinya Kusakari, Yoriaki Kaneko, Yoji Murata, Hiroshi Ohnishi, Yoshihisa Nojima, Kenji Takagishi, Takashi Matozaki

  Lead, Jun. 2012, JOURNAL OF IMMUNOLOGY, 188(11) (11), 5397 - 5407, English

  [Refereed]

  Scientific journal


• Signal regulatory protein α regulates the homeostasis of T lymphocytes in the spleen

  Miho Sato-Hashimoto, Yasuyuki Saito, Hiroshi Ohnishi, Hiroko Iwamura, Yoshitake Kanazawa, Tetsuya Kaneko, Shinya Kusakari, Takenori Kotani, Munemasa Mori, Yoji Murata, Hideki Okazawa, Carl F. Ware, Per-Arne Oldenborg, Yoshihisa Nojima, Takashi Matozaki

  Jul. 2011, Journal of Immunology, 187(1) (1), 291 - 297, English

  [Refereed]

  Scientific journal


• Essential roles of SIRPα in homeostatic regulation of skin dendritic cells

  Hiroko Iwamura, Yasuyuki Saito, Miho Sato-Hashimoto, Hiroshi Ohnishi, Yoji Murata, Hideki Okazawa, Yoshitake Kanazawa, Tetsuya Kaneko, Shinya Kusakari, Takenori Kotani, Yoshihisa Nojima, Takashi Matozaki

  Mar. 2011, Immunology Letters, 135(1-2) (1-2), 100 - 107, English

  [Refereed]

  Scientific journal


• Expression, localization, and biological function of the R3 subtype of receptor-type protein tyrosine phosphatases in mammals

  Takashi Matozaki, Yoji Murata, Munemasa Mori, Takenori Kotani, Hideki Okazawa, Hiroshi Ohnishi

  Dec. 2010, CELLULAR SIGNALLING, 22(12) (12), 1811 - 1817, English

  [Refereed]

  Scientific journal


• Role of SIRP alpha in regulation of mucosal immunity in the intestine

  Yoshitake Kanazawa, Yasuyuki Saito, Yana Supriatna, Hiroyuki Tezuka, Takenori Kotani, Yoji Murata, Hideki Okazawa, Hiroshi Ohnishi, Yoshitaka Kinouchi, Yoshihisa Nojima, Toshiaki Ohteki, Tooru Shimosegawa, Takashi Matozaki

  Dec. 2010, GENES TO CELLS, 15(12) (12), 1189 - 1200, English

  [Refereed]

  Scientific journal


• Requirement of SIRP alpha for protective immunity against Leishmania major

  Naoko Morimoto, Yoji Murata, Sei-ichiro Motegi, Kazutomo Suzue, Yasuyuki Saito, Hideki Okazawa, Hiroshi Ohnishi, Takenori Kotani, Shinya Kusakari, Osamu Ishikawa, Takashi Matozaki

  Oct. 2010, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 401(3) (3), 385 - 389, English

  [Refereed]

  Scientific journal


• Promotion of Cell Spreading and Migration by Vascular Endothelial-Protein Tyrosine Phosphatase (VE-PTP) in Cooperation With Integrins

  Munemasa Mori, Yoji Murata, Takenori Kotani, Shinya Kusakari, Hiroshi Ohnishi, Yasuyuki Saito, Hideki Okazawa, Tamotsu Ishizuka, Masatomo Mori, Takashi Matozaki

  Jul. 2010, JOURNAL OF CELLULAR PHYSIOLOGY, 224(1) (1), 195 - 204, English

  [Refereed]

  Scientific journal


• Tyrosine phosphorylation of R3 subtype receptor-type protein tyrosine phosphatases and their complex formations with Grb2 or Fyn

  Yoji Murata, Munemasa Mori, Takenori Kotani, Yana Supriatna, Hideki Okazawa, Shinya Kusakari, Yasuyuki Saito, Hiroshi Ohnishi, Takashi Matozaki

  May 2010, GENES TO CELLS, 15(5) (5), 513 - 524, English

  [Refereed]

  Scientific journal


• Expression of PTPRO in the Interneurons of Adult Mouse Olfactory Bulb

  Takenori Kotani, Yoji Murata, Hiroshi Ohnishi, Munemasa Mori, Shinya Kusakari, Yasuyuki Saito, Hideki Kazawa, John L. Bixby, Takashi Matozaki

  Lead, Jan. 2010, JOURNAL OF COMPARATIVE NEUROLOGY, 518(2) (2), 119 - 136, English

  [Refereed]

  Scientific journal


• Constitutive activation of neuronal Src causes aberrant dendritic morphogenesis in mouse cerebellar Purkinje cells

  Takenori Kotani, Nobuhiro Morone, Shigeki Yuasa, Shigeyuki Nada, Masato Okada

  Lead, Feb. 2007, NEUROSCIENCE RESEARCH, 57(2) (2), 210 - 219, English

  [Refereed]

  Scientific journal

• CD47-SIRPαシグナル系を標的としたマクロファージによるがん細胞排除を促進する新規大環状ペプチドの開発(A novel macrocyclic peptide targets the CD47-SIRPalpha axis and enhances the macrophage-mediated tumor killing)

  羽間 大祐, 村田 陽二, 田中 大介, 岡本 武士, 齊藤 泰之, 小谷 武徳, 西村 善博, 的崎 尚

  日本癌学会, Sep. 2019, 日本癌学会総会記事, 78回, P - 2169, English


• SIRPα+ dendritic cells regulate homeostasis of fibroblastic reticular cells in the adult spleen

  SAITO Yasuyuki, KOMORI Satomi, DATU Respatika, WASHIO Ken, NISHIMURA Taichi, KOTANI Takenori, MURATA Yoji, MATOZAKI Takashi

  30 Sep. 2018, 臨床血液, 59(9) (9), 1687, English


• CD47-signal regulatory protein α signaling system and its application to cancer immunotherapy.

  Yoji Murata, Yasuyuki Saito, Takenori Kotani, Takashi Matozaki

  Tumor cells evade immune surveillance through direct or indirect interactions with various types of immune cell, with much recent attention being focused on modifying immune cell responses as the basis for the development of new cancer treatments. Signal regulatory protein α (SIRPα) and CD47 are both transmembrane proteins that interact with each other and constitute a cell-cell communication system. SIRPα is particularly abundant in myeloid cells such as macrophages and dendritic cells, whereas CD47 is expressed ubiquitously and its expression level is elevated in cancer cells. Recent studies have shown that blockade of CD47-SIRPα interaction enhances the phagocytic activity of phagocytes such as macrophages toward tumor cells in vitro as well as resulting in the efficient eradication of tumor cells in a variety of xenograft or syngeneic mouse models of cancer. Moreover, CD47 blockade has been shown to promote the stimulation of tumor-specific cytotoxic T cells by macrophages or dendritic cells. Biological agents, such as Abs and recombinant proteins, that target human CD47 or SIRPα have been developed and are being tested in preclinical models of human cancer or in clinical trials with cancer patients. Preclinical studies have also suggested that CD47 or SIRPα blockade may have a synergistic antitumor effect in combination with immune checkpoint inhibitors that target the adaptive immune system. Targeting of the CD47-SIRPα signaling system is thus a promising strategy for cancer treatment based on modulation of both innate and acquired immune responses to tumor cells.

  Aug. 2018, Cancer science, 109(8) (8), 2349 - 2357, English, International magazine

  [Refereed]

  Link to Kobe Univ. Repository (Kernel)


• Role of intestinal epithelial Src family kinases in the control of intestinal inflammation

  SUN Chunxiao, SUN Chunxiao, MURATA Yoji, IMADA Shinya, KOTANI Takenori, SAITO Yasuyuki, YAMADA Hideto, MATOZAKI Takashi

  2018, 日本癌学会学術総会抄録集(Web), 77th, ROMBUNNO.P‐3042 (WEB ONLY), English


• Regulation of small intestinal homeostasis by Tsc2‐mTORC1 signaling

  JAJAR Setiawan, KOTANI Takenori, KONNO Tasuku, MURATA Yoji, SAITO Yasuyuki, MATOZAKI Takashi

  2018, 日本癌学会学術総会抄録集(Web), 77th, ROMBUNNO.P‐1102 (WEB ONLY), English


• Srcファミリーキナーゼの炎症性腸疾患における重要性

  SUN Chunxiao, SUN Chunxiao, MURATA Yoji, IMADA Shinya, KOTANI Takenori, SAITO Yasuyuki, YAMADA Hideto, MATOZAKI Takashi

  2018, 日本生化学会大会(Web), 91st, ROMBUNNO.1P‐297 (WEB ONLY), English


• 腸上皮細胞の恒常生維持に関わるチロシンリン酸化シグナルの意義

  的崎尚, 村田陽二, 小谷武徳, 齊藤泰之

  2018, 日本生化学会大会(Web), 91st, ROMBUNNO.1S03e‐02 (WEB ONLY), Japanese


• 常在細菌による成体マウス脳室下帯におけるニューロン新生の制御

  澤田直樹, 澤田直樹, 小谷武徳, 金野祐, ジャジャル セティアワン, 齊藤泰之, 村田陽二, 丹生健一, 的崎尚

  2018, 日本生化学会大会(Web), 91st, ROMBUNNO.2T13m‐01(2P‐325) (WEB ONLY), Japanese


• SIRP alpha(+) dendritic cells regulate homeostasis of fibroblastic reticular cells via TNF receptor ligands in the adult spleen

  Yasuyuki Saito, Satomi Komori, Datu Respatika, Ken Washio, Takenori Kotani, Yoji Murata, Hiroshi Ohnishi, Katsuyuki Yui, Koji Yasutomo, Takashi Matozaki

  Dec. 2017, CYTOKINE, 100, 55 - 55, English

  Summary international conference


• 腸上皮の恒常性制御におけるTsc2‐mTORC1シグナルの役割

  SETIAWAN Jajar, KOTANI Takenori, KONNO Tasuku, IMADA Shinya, PARK Jung‐ha, SAWADA Naoki, SAITO Yasuyuki, MURATA Yoji, MATOZAKI Takashi

  2017, 日本生化学会大会(Web), 90th, ROMBUNNO.2P‐0458 (WEB ONLY), English


• Anti‐SIRPα antibodies provide a basis for new approaches to cancer immunotherapy

  MATOZAKI Takashi, MURATA Yoji, YANAGITA Tadahiko, SAITO Yasuyuki, KOTANI Takenori

  2017, 日本癌学会学術総会抄録集(Web), 76th, ROMBUNNO.S5‐3 (WEB ONLY), English


• Establishment of a potential new PDX mouse model for personalized cancer immunotherapy

  SAITO Yasuyuki, MURATA Yoji, KOTANI Takenori, FLAVELL Richard A, MANZ Markus G, MATOZAKI Takashi

  2017, 日本癌学会学術総会抄録集(Web), 76th, ROMBUNNO.J‐1040 (WEB ONLY), English


• Regulation of intestinal epithelial homeostasis by Src family kinases through Rac and YAP

  MURATA Yoji, IMAGE Shinya, KOTANI Takenori, SUN Chunxiao, SAITO Yasuyuki, MATOZAKI Takashi

  2017, 日本癌学会学術総会抄録集(Web), 76th, ROMBUNNO.E‐1007 (WEB ONLY), English


• Role of Tsc2‐mTORC1 signaling in intestinal homeostasis

  KOTANI Takenori, JAJAR Setiawan, KONNO Tasuku, IMADA Shinya, SAWADA Naoki, MURATA Yoji, SAITO Yasuyuki, MATOZAKI Takashi

  2017, 日本癌学会学術総会抄録集(Web), 76th, ROMBUNNO.P‐3032 (WEB ONLY), English


• がん‐マクロファージ間相互作用を標的とした新規がん免疫療法の開発

  村田陽二, 柳田匡彦, 田中大介, 羽間大祐, 齊藤泰之, 小谷武徳, 大西浩史, 的崎尚

  2017, 日本生化学会大会(Web), 90th, ROMBUNNO.3AT26‐02(3P‐0901) (WEB ONLY), Japanese


• Future therapeutic potential of SAP-1 in inflammatory bowel diseases.

  Takenori Kotani, Yoji Murata, Yasuyuki Saito, Takashi Matozaki

  Dec. 2016, Expert review of gastroenterology & hepatology, 10(12) (12), 1313 - 1315, English, International magazine

  [Refereed]


• 短鎖脂肪酸は腸上皮細胞のターンオーバーを調節する(Short chain fatty acids regulate the turnover of intestinal epithelial cells)

  金野 祐, 小谷 武徳, Park Jung-Ha, Setiawan Jajar, 北村 泰明, 今田 慎也, 臼井 佑太郎, 波多野 直哉, 篠原 正和, 齊藤 泰之, 村田 陽二, 的崎 尚

  (公社)日本生化学会, Sep. 2016, 日本生化学会大会プログラム・講演要旨集, 89回, [2P - 238], Japanese


• The anti‐SIRPα antibody prevents tumor formation: a novel strategy for cancer therapy

  YANAGITA Tadahiko, MURATA Yoji, TANAKA Daisuke, SAITO Yasuyuki, KOTANI Takenori, KOMORI Takahide, MATOZAKI Takashi

  2016, 日本癌学会学術総会抄録集(Web), 75th, ROMBUNNO.J‐1004 (WEB ONLY), English


• SIRPα欠損マウスはLPS投与によって誘導される低体温症が重症化する

  HASHIMOTO Miho, NOZU Tomomi, URANO Eriko, SAITO Yasuyuki, KOTANI Takenori, MURATA Yoji, MATOZAKI Takashi, ONISHI Hiroshi

  2016, 日本生物学的精神医学会(Web), 38th, 154 (WEB ONLY), English


• SIRPα欠損マウスにおけるクプリゾン感受性の亢進

  野津智美, 橋本美穂, ELHANBALY Ruwaida, 石川達也, 齊藤泰之, 小谷武徳, 村田陽二, 深澤有吾, 的崎尚, 大西浩史

  2016, 北関東医学会総会プログラム・抄録, 63rd, 27‐28, Japanese


• 細胞間シグナルCD47‐SIRPα系の生理機能とその臨床応用

  的崎尚, 村田陽二, 齊藤泰之, 小谷武徳, 柳田匡彦, 田中大介, 大西浩史

  2016, 日本生化学会大会(Web), 89th, ROMBUNNO.1S10‐6 (WEB ONLY), Japanese


• 白質におけるミクログリア恒常性制御

  野津智美, 橋本美穂, ELHANBALY Ruwaida, 石川達也, 齊藤泰之, 小谷武徳, 村田陽二, 深澤有吾, 的崎尚, 大西浩史

  2016, 日本生物学的精神医学会(Web), 38th, 149 (WEB ONLY), Japanese


• 【細胞シグナル操作法】分子からみたシグナル操作法 キナーゼ プロテインホスファターゼ

  Kotani Takenori, Murata Yoji, Matozaki Takashi

  Oct. 2015, 生体の科学, 66(5号) (5号), 432 - 433, Japanese

  [Invited]

  Introduction commerce magazine


• 【プロテインホスファターゼの最先端:制御機構から医療まで】 受容体型チロシンホスファターゼの機能と病態 R3サブタイプチロシンホスファターゼを中心に

  Murata Yoji, Kotani Takenori, Matozaki Takashi

  日本生化学会, Oct. 2015, 生化学, 87(5号) (5号), 547 - 553, Japanese

  [Invited]

  Introduction scientific journal


• ミクログリアにおけるSIRPαシグナルの役割

  橋本美穂, 野津智美, 浦野江里子, 齊藤泰之, 小谷武徳, 村田陽二, 的崎尚, 大西浩史

  北関東医学会, 01 Aug. 2015, 北関東医学会総会プログラム・抄録, 62nd(3) (3), 20 - 21, Japanese


• 腸粘膜上皮細胞の微絨毛に局在する受容体型チロシンホスファターゼSAP‐1とCEACAM20による腸管免疫制御

  村田陽二, 小谷武徳, YANA Supriatna, 北村泰明, 今田慎也, 齊藤泰之, 大西浩史, 的崎尚

  05 Jun. 2015, 日本細胞生物学会大会要旨集, 67th, 175, Japanese


• 腸内細菌による腸上皮細胞の寿命制御

  小谷武徳, 朴貞河, 北村泰明, 北村泰明, 今田慎也, 村田陽二, 齊藤泰之, 的崎尚

  05 Jun. 2015, 日本細胞生物学会大会要旨集, 67th, 199, Japanese


• Importance of commensal bacteria and short‐chain fatty acids for the growth and migration of intestinal epithelial cells

  KOTANI Takenori, PARK Jung‐ha, KITAMURA Yasuaki, IMADA Shinya, MURATA Yoji, SAITO Yasuyuki, MATOZAKI Takashi

  2015, 日本癌学会学術総会抄録集(Web), 74th, J‐1203 (WEB ONLY), English


• Cell‐cell interactions via CD47‐SIRPα signal regulate microglial activation

  HASHIMOTO Miho, NOZU Tomomi, URANO Eriko, SAITO Yasuyuki, KOTANI Takenori, MURATA Yoji, MATOZAKI Takashi, OHNISHI Hiroshi

  2015, 日本神経化学会大会抄録集(Web), 58th, ROMBUNNO.2P‐34 (WEB ONLY), English


• 腸上皮細胞の増殖・移動制御における腸内細菌と短鎖脂肪酸の重要性

  KOTANI Takenori, PARK Jung‐ha, KITAMURA Yasuaki, IMADA Shinya, MURATA Yoji, SAITO Yasuyuki, MATOZAKI Takashi

  2015, 日本生化学会大会(Web), 88th, 3P0174 (WEB ONLY), English


• 腸上皮組織の恒常性制御におけるCskとSrcファミリーキナーゼの役割

  IMADA Shinya, IMADA Shinya, MURATA Yoji, KITAMURA Yasuaki, PARK Jung‐ha, HATANO Masaki, KONNO Tasuku, KOTANI Takenori, SAITO Yasuyuki, OHDAN Hideki, MATOZAKI Takashi

  2015, 日本生化学会大会(Web), 88th, 2T21P-14(2P0206) (WEB ONLY), English


• Role of C‐terminal Src kinase (Csk) and Src family kinases in homeostasis of the intestinal epithelium

  IMADA Shinya, IMADA Shinya, MURATA Yoji, KITAMURA Yasuaki, PARK Jung‐ha, KOTANI Takenori, SAITO Yasuyuki, OHDAN Hideki, MATOZAKI Takashi

  2015, 日本癌学会学術総会抄録集(Web), 74th, J‐1283 (WEB ONLY), English


• Essential role of SIRP α on dendritic cells in organization and homeostasis of the spleen

  WASHIO Ken, KOTANI Takenori, RESPATIKA Datu, MURATA Yoji, SAITO Yasuyuki, KANEKO Yoriaki, OKAZAWA Hideki, OHNISHI Hiroshi, FUKUNAGA Atsushi, NISHIGORI Chikako, MATOZAKI Takashi

  18 Nov. 2014, 日本免疫学会総会・学術集会記録, 43(Proceedings) (Proceedings), 38, English


• 細胞間コミュニケーションシステムCD47‐SIRPα系による血液・免疫系の制御

  小谷武徳, 鷲尾健, ダトゥレス パティカ, 村田陽二, 齊藤泰之, 的崎尚

  2014, 日本分子生物学会年会プログラム・要旨集(Web), 37th, 2W9-3(2P-0718) (WEB ONLY), Japanese


• 細胞質型チロシンホスファターゼShp2の成熟脳における機能解析

  草刈 伸也, 橋本 美穂, 柴崎 貢志, 吾郷 由希夫, 松田 敏夫, Neel Benjamin G., 小谷 武徳, 村田 陽二, 的崎 尚, 大西 浩史

  北関東医学会, 01 Aug. 2013, The Kitakanto medical journal = 北関東医学, 63(3) (3), 321 - 322, Japanese


• SIRPαによる脾臓T細胞の恒常性の調節

  橋本美穂, 齊藤泰之, 金子哲也, 大西浩史, 草苅伸也, 小谷武徳, 村田陽二, 岡澤秀樹, 的崎尚

  北関東医学会, 01 Aug. 2013, 北関東医学会総会プログラム・抄録, 60th(3) (3), 24 - 25, Japanese


• IMPORTANCE OF THE PROTEIN TYROSINE PHOSPHATASE SHP1 IN DENDRITIC CELLS FOR PREVENTION OF TH1 CELL DIFFERENTIATION AND AUTOIMMUNITY: A POTENTIAL TARGET FOR THE THERAPY

  T. Kaneko, Y. Saito, T. Kotani, H. Ohnishi, Y. Murata, Y. Yonemoto, K. Okamura, T. Matozaki, K. Takagishi

  Jun. 2012, ANNALS OF THE RHEUMATIC DISEASES, 71, 73 - 73, English

  Summary international conference


• 脾臓T細胞の恒常性調節へのSIRPαの関与

  橋本(佐藤)美穂, 齊藤泰之, 金子哲也, 大西浩史, 草苅伸也, 小谷武徳, 村田陽二, 岡澤秀樹, 的崎尚

  2012, 日本生化学会大会(Web), 85th, 3T29-06 (WEB ONLY), Japanese


• Dendritic cell‐specific depletion of protein tyrosine phosphatase Shp1 promotes Th1 differentiation causes autoimmunit

  KANEKO Tetsuya, SAITO Yasuyuki, OHNISHI Hiroshi, SATO‐HASHIMOTO Miho, KOTANI Takenori, MURATA Yoji, OKAZAWA Hideki, TAKAGISHI Kenji, MATOZAKI Takashi

  07 Nov. 2011, 日本免疫学会総会・学術集会記録, 40, 199, English


• 血管内皮細胞のシェアストレス刺激応答におけるVE‐PTPの機能解析

  村田陽二, 森宗昌, 小谷武徳, 草苅伸也, 岡澤秀樹, 齊藤泰之, 大西浩史, 的崎尚

  2010, 生化学, ROMBUNNO.4T9-2, Japanese


• 腸微絨毛特異的な発現を示す受容体型チロシンホスファターゼSAP‐1とIL‐10による炎症性腸疾患の制御

  小谷武徳, 村田陽二, YANA Supriatna, 岡澤秀樹, 大西浩史, EDWIN Daniwijaya, 定方久延, 草苅伸也, 齊藤泰之, 大川克也, 的崎尚

  2010, 生化学, ROMBUNNO.4P-0447, Japanese


• 腸微絨毛特異的な発現を示す受容体型チロシンホスファターゼSAP‐1とIL‐10による炎症性腸疾患の制御

  小谷武徳, 村田陽二, YANA Supriatna, 大西浩史, 定方久延, 草苅伸也, 齊藤泰之, 岡澤秀樹, 的崎尚

  25 Sep. 2009, 生化学, ROMBUNNO.4T5A-16, Japanese


• Promotion by VE‐PTP of lamellipodium formation in cooperation with Ras and integrin

  MORI Munemasa, MURATA Yoji, OHNISHI Hiroshi, OKAZAWA Hideki, KOTANI Takenori, SAITO Yasuyuki, MORI Masatomo, MATOZAKI Takashi

  31 Aug. 2009, 日本癌学会学術総会記事, 68th, 246, English


• Tyrosine phosphorylation of SAP‐1, a receptor‐type protein tyrosine phosphatase, and its association with Grb2

  MURATA Yoji, KOTANI Takenori, SUPRIATNA Yana, MORI Munemasa, OKAZAWA Hideki, OHNISHI Hiroshi, SAITO Yasuyuki, MATOZAKI Takashi

  31 Aug. 2009, 日本癌学会学術総会記事, 68th, 204, English

• Role of CD47 on the regulation of peripheral T cell survival by dendritic cells

  Satomi Komori, Yasuyuki Saito, Taichi Nishimura, Hiroki Yoshida, Risa Sugihara, Rie Iida, Takenori Kotani, Yoji Murata, Takashi Matozaki

  14th International Conference on Protein Phosphatase, Dec. 2020, English, International conference

  Poster presentation


• Antibodies to SIRPα/SIRPβ as a potential tool for immunotherapy of bladder cancer

  Mariko Sakamoto, Yoji Murata, Yuka Kakuchi, Daisuke Takana, Takeshi Okamoto, Yasuyuki Saito, Takenori Kotani, Masato Fujisawa, Takashi Matozaki

  14th International Conference on Protein Phosphatase, Dec. 2020, English, International conference

  Oral presentation


• Blockade of the CD47-SIRPα interaction by SIRPα-binding macrocyclic peptides as a potential cancer immunotherapy

  Yoji Murata, Daisuke Hazama, Yizhen Yin, Mariko Sakamoto, Yasuyuki Saito, Takenori Kotani, Hiroaki Suga, Takashi Matozaki

  14th International Conference on Protein Phosphatase, Dec. 2020, International conference

  Oral presentation


• Roles of Shp2-Ras, Src family kinases, and mTOR signaling in intestinal epithelial homeostasis

  Takenori Kotani, Saki Okamoto, Yoji Murata, Yasuyuki Saito, Takashi Matozaki

  14th International Conference on Protein Phosphatase, Dec. 2020, English, International conference

  Oral presentation


• SIRPα targeting as a potential therapy of Langerhans cell histiocytosis-

  岡本 武士, 村田 陽二, 羽間 大祐, 坂本 茉莉子, 角地 宥香, 齊藤 泰之, 小谷 武徳, 眞庭 謙昌, 的崎 尚

  第79回日本癌学会学術総会, Oct. 2020, Domestic conference

  Oral presentation


• Role of K-Ras activity in regulation of proliferation and differentiatiojn of intestinal epithelial cells

  小谷 武徳, 井原 紀子, ジャジャル セティアワン, 岡本 沙樹, 齊藤 泰之, 村田 陽二, 的崎 尚

  第79回日本癌学会学術総会, Oct. 2020, Japanese, Domestic conference

  Oral presentation


• The novel antitumor effect of an antibody to SIRPα/SIRPβagainst bladder cancers

  坂本 茉莉子, 村田 陽二, 羽間 大祐, 岡本 武士, 角地 宥香, 齊藤 泰之, 小谷 武徳, 藤沢 正人, 的崎 尚

  第79回日本癌学会学術総会, Oct. 2020, Japanese, Domestic conference

  Oral presentation


• Humanized mouse models for in vivo evaluation of cancer immunotherapy targeting human macrophages

  Yasuyuki Saito, Rie Iida, Daisuke Hazama, Takenori Kotani, Yoji Murata, Takashi Matozaki

  第79回日本癌学会学術総会, Oct. 2020, English, Domestic conference

  Oral presentation


• 膜型分子CD47を介した成熟T細胞の寿命制御

  小森 里美, 齊藤 泰之, 西村 太一, 吉田 弘樹, 杉原 理紗, 飯田 理恵, 小谷 武徳, 村田 陽二, 的崎 尚

  第19回生体機能研究会, Sep. 2020, International conference

  Oral presentation


• Srcﾌｧﾐﾘｰｷﾅｰｾﾞ、Ras, ｍTORC1ｼｸﾞﾅﾙによる腸上皮細胞の恒常性制御

  小谷 武徳, 岡本 沙樹, 村田 陽二, 齊藤 泰之, 的崎 尚

  第19回生体機能研究会, Sep. 2020, Domestic conference

  Oral presentation


• 抗SIRPα抗体を用いた膀胱がんに対する新規がん免疫療法開発

  坂本 茉莉子, 村田 陽二, 田中 大介, 岡本 武士, 羽間 大祐, 角地 宥加, 齊藤 泰之, 小谷 武徳, 的崎 尚

  第19回生体機能研究会, Sep. 2020, Domestic conference

  Oral presentation


• Identification of macrocyclic peptides that regulate the CD47-SIRPαsystem, and their potential as anti-cancer drugs

  村田 陽二, 羽間 大祐, Yin Yizhen, 松田 真, 岡本 武士, 田中 大介, 坂本 茉莉子, 角地 宥香, 寺坂 尚紘, Zhao Jinxuan, 齊藤 泰之, 小谷 武徳, 中川 敦史, 菅 裕明, 的崎 尚

  第93回日本生化学会大会, Sep. 2020, Domestic conference

  Oral presentation


• Role of K-Ras activity in regulation of intestinal proliferation and differentiatiojn of intestinal epithelial cells

  小谷 武徳, 井原 紀子, ジャジャル セティアワン, 岡本 沙樹, 齊藤 泰之, 村田 陽二, 的崎 尚

  第93回日本生化学会大会, Sep. 2020, Domestic conference

  Poster presentation


• A nobel macrocycle peptide targets CD47-SIRPalpha axis and enhances the macrophage-mediated tumor killing

  Daisuke Hazama, Yoji Murata, Daisuke Tanaka, Takeshi Okamoto, Ysuyuki Saito, Takenori Kotani, Yoshihiro Nishimura, Takashi Matozaki

  第78回日本癌学会総会, Sep. 2019, Japanese

  Poster presentation


• SIRPα抗体を用いた新規のがん免疫療法:CD20及びPD-1抗体との併用による有効性

  Takashi Matozaki, Yoji Murata, Yasuyuki Saito, Takenori Kotani

  第78回日本癌学会総会, Sep. 2019, Japanese

  Oral presentation


• Role of Tsc2-mTORC1 signaling in regulation of colonic epithelial homeostasis

  Takenori Kotani, Jajar Setiawan, Noriko Ihara, Saki Okamoto, Yoji Murata, Yasuyuki Saito, Takashi Matozaki

  第78回日本癌学会総会, Sep. 2019, Japanese

  Poster presentation


• Regulation of innate immune cell functions by the SHP-1/2 binding protein SIRPα

  村田 陽二, 齊藤 泰之, 小谷 武徳, 的崎 尚

  第92回日本生化学会大会, Sep. 2019, Japanese

  Public symposium


• Regulation by macrophages of the hematopoietic cell lifespan and its therapeutic application

  的崎 尚, 齊藤 泰之, 小谷 武徳, 村田 陽二

  第92回日本生化学会大会, Sep. 2019, Japanese

  Public symposium


• Importance of SIRPα+ dendric cells for the development of fibroblastic reticular cells of peripheral lymph nodes

  Satomi Komori, Yasuyuki Saito, Datu Resppatika, Taichi Nishimura, Takenori Kotani, Yoji Murata, Takashi Matozaki

  第92回日本生化学会大会, Sep. 2019, English

  Poster presentation


• 新規SIRPα阻害剤の開発とその抗腫瘍効果

  村田 陽二, 羽間 大祐, 田中 大介, 岡本 武士, 齊藤 泰之, 小谷 武徳, 的崎 尚

  第18回生体機能研究会, Jul. 2019, Japanese

  Oral presentation


• SIRPα陽性樹状細胞による末梢リンパ節ストローマ細胞の発達制御

  小森 里美, 齊藤 泰之, ダトゥ レスパティカ, 西村 太一, 小谷 武徳, 村田 陽二, 的崎 尚

  第9回ﾎｽﾌｧﾀｰｾﾞ研究会学術集会, Jul. 2019, Japanese

  Oral presentation


• 膜型分子SIRPαを標的とする新たな免疫チェックポイント阻害剤の開発

  羽間 大祐, 村田 陽二, 田中 大介, 岡本 武士, 齊藤 泰之, 小谷 武徳, 西村 善博, 的崎 尚

  第9回ﾎｽﾌｧﾀｰｾﾞ研究会学術集会, Jul. 2019, Japanese

  Oral presentation


• 大腸上皮の恒常性制御におけるTsc2-mTORC1シグナルの役割

  小谷 武徳, ジャジャル セティアワン, 井原 紀子, 岡本 沙樹, 村田 陽二, 齊藤 泰之, 的崎 尚

  第9回ﾎｽﾌｧﾀｰｾﾞ研究会学術集会, Jul. 2019, Japanese

  Oral presentation


• Role of Tsc2-mTORC1 signaling in homeostasis of intestinal epithelium and its relation to inflammation and cancer

  Takenori Kotani, Jajar Setiawan, Yoji Murata, Yasuyuki Saito, Takashi Matozaki

  AACR Annual Meeting 2019, Mar. 2019, English, the American Association for Cancer Research, アトランタ, International conference

  Poster presentation


• SIRPα＋Dendritic Cells regulate organization of lymph node stromal sells in vivo

  Satomi Komori, Yasuyuki Saito, Respatika Datu, Takenori Kotani, Yoji Murata, Takashi Matozaki

  第47回日本免疫学学術集会, Dec. 2018, English, 日本免疫学会, 福岡, Domestic conference

  Oral presentation


• Importance of SIRPα on dendritic cells for the development of exprerimental autoimmune encephalomyelitis

  Taichi Nishimura, Yasuyuki Saito, Satomi Komori, Respatika Datu, Ken Washio, Takenori Kotani, Yoji Murata, Satoshi Mizobuchi, Takashi Matozaki

  第47回日本免疫学学術集会, Dec. 2018, English, 日本免疫学会, 福岡, Domestic conference

  Oral presentation


• SIRPα陽性樹状細胞による脾臓細網線維芽細胞の恒常性の制御

  SAITO YASUYUKI, 小森 里美, Datu Respatika, 鷲尾 健, 西村 太一, KOTANI TAKENORI, MURATA YOJI, MATOZAKI TAKASHI

  第80回日本血液学会学術集会, Oct. 2018, English, 日本血液学会, 大阪, Domestic conference

  Poster presentation


• Role of the tyrosine phosphorylation signal in homeostasis of intestinal epithelium and its relation to inflammation and cancer

  Takashi Matozaki, Yoji Murata, Takenori Kotani, Yasuyuki Saito

  Workshop on Frontiers in Phosphatase Research and Drug Discovery, Oct. 2018, English, 日本プロテインホスファターゼ研究会, 東京, International conference

  Oral presentation


• 腸上皮細胞の恒常生維持に関わるチロシンリン酸化シグナルの意義

  MATOZAKI TAKASHI, MURATA YOJI, KOTANI TAKENORI, SAITO YASUYUKI

  第91回日本生化学会大会, Sep. 2018, English, 日本生化学会, 京都, Domestic conference

  Oral presentation


• 腸炎症制御における腸上皮Srcファミリーキナーゼの役割

  孫 春暁, MURATA YOJI, 今田 慎也, KOTANI TAKENORI, SAITO YASUYUKI, YAMADA HIDETO, MATOZAKI TAKASHI

  第77回日本癌学会学術総会, Sep. 2018, English, 日本癌学会, 大阪, Domestic conference

  Poster presentation


• 常在細菌による成体マウス脳室下帯におけるニュートロン新生の制御

  澤田 直樹, KOTANI TAKENORI, 金野 祐, ジャジャル セティアワン, SAITO YASUYUKI, MURATA YOJI, NIBU KENICHI, MATOZAKI TAKASHI

  第91回日本生化学会大会, Sep. 2018, English, 日本生化学会, 京都, Domestic conference

  Poster presentation


• Tsc2-mTORC1シグナルによる小腸上皮の恒常性制御

  ジャジャル セティアワン, KOTANI TAKENORI, 金野 祐, MURATA YOJI, SAITO YASUYUKI, MATOZAKI TAKASHI

  第77回日本癌学会学術総会, Sep. 2018, Japanese, 日本癌学会, 大阪, Domestic conference

  Poster presentation


• Srcファミリーキナーゼの炎症性腸疾患における重要性

  孫 春暁, MURATA YOJI, 今田 慎也, KOTANI TAKENORI, SAITO YASUYUKI, YAMADA HIDETO, MATOZAKI TAKASHI

  第91回日本生化学会大会, Sep. 2018, Japanese, 日本生化学会, 京都, Domestic conference

  Poster presentation


• 実験的自己免疫性脳脊髄炎の発症制御におけるCD47-SIRPα系の役割について

  西村 太一, SAITO YASUYUKI, 鷲尾 健, 小森 里美, KOTANI TAKENORI, MURATA YOJI, MIZOBUCHI SATOSHI, MATOZAKI TAKASHI

  第17回生体機能研究会, Jul. 2018, Japanese, 生体機能研究会, 渋川, Domestic conference

  Oral presentation


• 抗SIRPα抗体による新規がん免疫療法

  羽間 大祐, MURATA YOJI, 栁田 匡彦, 田中 大介, SAITO YASUYUKI, KOTANI TAKENORI, NISHIMURA YOSHIHIRO, MATOZAKI TAKASHI

  第17回生体機能研究会, Jul. 2018, Japanese, 生体機能研究会, 渋川, Domestic conference

  Oral presentation


• SIRPα＋Dendritic Cells regulate homeostasis of fibroblastic reticular cells via TNF receptor ligands in the adult spleen

  Yasuyuki Saito, Datu Rrspatika, Satomi Komori, Ken Washio, Taichi Nishimura, Takenori Kotani, Yoji Murata, Takashi Matozaki

  15th International Symposium on Dendritic Cells, Jun. 2018, Japanese, Aachen, International conference

  Poster presentation


• 成体マウス脳室下帯における神経新生の常在細菌による制御

  澤田 直樹, KOTANI TAKENORI, 金野 祐, Jajar Setiawan, 齊藤 泰之, 村田, 陽二, 的崎 尚

  第65回日本生化学会近畿支部例会, May 2018, English, The Japanese Biochemical Society, Kinki Branch, 西宮, Domestic conference

  Poster presentation


• Role of Csk iRole of Csk in the regulation of intestinal homeostasis and the development of inflammation in the colonn the regulation of intestinal homeostasis and the development of inflammation in the colon

  Chunxiao Sun, Yoji Murata, Shinya Imada, Takenori Kotani, Yasuyuki Saito, Hideto Yamada, Takashi Matozaki

  第65回日本生化学会近畿支部例会, May 2018, Japanese, The Japanese Biochemical Society, Kinki Branch, 西宮, Domestic conference

  Poster presentation


• SIRPα+ dendritic cells regulate homeostasis of fibroblastic reticular cells via TNF receptor ligands in the adult spleen

  Satomi Komori, Saito Yasuyuki, Datu Respatika, Kotani Takenori, Murata Yoji, Matozaki Takashi

  The 2nd International Joint Symposium -UW,UO,KU-, Mar. 2018, English, Center for Cell Signaling and Medical Innovation, Honolulu, USA(Hawaii), International conference

  Poster presentation


• Regulation of intestinal epithelial cell turnover by intertinal contents

  Konno Tasuku, Kotani Takenori, Jung-ha Park, Jajar Setiawan, Naoki Sawada, Yuka Nishigaito, Naoya, Hatano, Masato Okada, Shinohara Masakazu, Saito Yasuyuki, Murata Yoji, Matozaki Takashi

  The 2nd International Joint Symposium -UW,UO,KU-, Mar. 2018, English, Center for Cell Signaling and Medical Innovation, Honolulu, USA(Hawaii), International conference

  Poster presentation


• Development of a novel cancer immunotherapy targeting the interaction between tumor cells and macrophages

  Murata Yoji, Tadahiro Yanagita, Daisuke Tanaka, Daisuke Hazama, Saito Yasuyuki, Kotani Takenori, Hiroshi Ohnishi, Matozaki Takashi

  ConBio2017(The 90th Annual Meeting of the Japanese Biochemical Society, The 40th Annual Meeting of the Molecular Biology Society of Japan), Dec. 2017, Japanese, The Japanese Biochemical Society, The Molecular Biology Society of Japan, 神戸, Domestic conference

  Oral presentation


• SIRPα+ dendritic cells regulate homeostasis of fibroblastic reticular cells via TNF receptor ligands in the adult spleen

  Saito Yasuyuki, Satomi Komori, Datu Respatika, Washio Ken, Kotani Takenori, Murata Yoji, Hiroshi Ohnishi, Katsuyuki Yui, Koji Yasutomo, Matozaki Takashi

  The 46th Annual Meeting of The Japanese Society for Immunology, Dec. 2017, English, Japanese Society for Immunology, 仙台, Domestic conference

  Oral presentation


• Role of Tsc2-mTORC1 signaling in regulation of intestinal epithelial homeostasis

  Jajar Setiawan, Kotani Takenori, Konno Tasuku, Shinya Imada, Jung-ha Park, Naoki Sawada, Saito Yasuyuki, Murata Yoji, Matozaki Takashi

  ConBio2017(The 90th Annual Meeting of the Japanese Biochemical Society, The 40th Annual Meeting of the Molecular Biology Society of Japan), Dec. 2017, English, The Japanese Biochemical Society, The Molecular Biology Society of Japan, 神戸, Domestic conference

  Poster presentation


• Development of a novel primary culture system for lymphoid organ fibroblastic reticular cells

  Komori Satomi, Saito Yasuyuki, Datu Respatika, Kotani Takenori, Murata Yoji, Matozaki Takashi

  The 46th Annual Meeting of The Japanese Society for Immunology, Dec. 2017, English, Japanese Society for Immunology, 仙台, Domestic conference

  Poster presentation


• SIRPα+ dendritic cells regulate homeostasis of fibroblastic reticular cells via TNF receptor ligands in the adult spleen

  Saito Yasuyuki, Satomi Komori, Datu Respatika, Washio Ken, Kotani Takenori, Murata Yoji, Hiroshi Ohnishi, Katsuyuki Yui, Koji Yasutomo, Matozaki Takashi

  The 5th Annual Meeting of the International Cytokine and Interferon Society, ICIS 2017 5th Annual Meeting of the International Cytokine and Interferon Society, ICIS 2017, Nov. 2017, English, International Cytokine and Interferon Society, 金沢, International conference

  Oral presentation


• Role of mechanistic target of rapamycin complex 1 (mTORC1) in regulation of intestinal epithelial homeostasis

  Kotani Takenori, Jajar Setiawan, Konno Tasuku, Shinya Imada, Naoki Sawada, Murata Yoji, Saito Yasuyuki, Matozaki Takashi

  The 3rd Japan-Taiwan Bilateral Conference on Protein Phosphatase in Sendai. The 8th Japanese Conference on Protein Phosphatase., Nov. 2017, English, Japanese Association for Protein Phosphatase Research, 仙台, International conference

  Oral presentation


• 免疫系ヒト化マウスを用いた新規がん異種移植マウスモデルの作成

  Saito Yasuyuki, 羽間 大祐, Kotani Takenori, Murata Yoji, Matozaki Takashi

  なし, Sep. 2017, Japanese, なし, 岐阜, Domestic conference

  Oral presentation


• 二次リンパ組織ストローマ細胞の新規初代培養系の樹立と増殖・制御機構の解明

  小森 里美, Saito Yasuyuki, Datu Respatika, Kotani Takenori, Murata Yoji, Matozaki Takashi

  なし, Sep. 2017, Japanese, なし, 岐阜, Domestic conference

  Oral presentation


• 腸内容物による腸上皮細胞のターンオーバー制御

  Konno Tasuku, Kotani Takenori, 朴 貞河, Jajar Setiawan, 澤田 直樹, 西垣内 佑香, Hatano Naoya, 岡田 雅人, Shinohara Masakazu, Saito Yasuyuki, Murata Yoji, Matozaki Takashi

  なし, Sep. 2017, Japanese, なし, 岐阜, Domestic conference

  Oral presentation


• Role of Tsc2-mTORC1 signaling in intestinal homeostasis

  Kotani Takenori, Jajar Setiawan, Konno Tasuku, Shinya Imada, Naoki Sawada, Murata Yoji, Saito Yasuyuki, Matozaki Takashi

  The 76th Annual Meeting of the Japanese Cancer Association, Sep. 2017, Japanese, The Japanese Cancer Association, 横浜, Domestic conference

  Poster presentation


• Anti-SIRPalpha antibodies provide a basis for new approaches to canser immunotherapy,

  Matozaki Takashi, Murata Yoji, Tadahiro Yanagita, Saito Yasuyuki, Kotani Takenori

  The 76th Annual Meeting of the Japanese Cancer Association, Sep. 2017, English, The Japanese Cancer Association, 横浜, Domestic conference

  Oral presentation


• Establishment of a potential new PDX mouse model for personalized cancer immunotherapy

  Saito Yasuyuki, Murata Yoji, Kotani Takenori, Richard A. Flavell, Markus G. Manz, Matozaki Takashi

  The 76th Annual Meeting of the Japanese Cancer Association, Sep. 2017, Japanese, The Japanese Cancer Association, 横浜, Domestic conference

  Oral presentation


• Regulation of interestinal epithelial homeostasis by Src family kinases through Rac and YAP

  Murata Yoji, Shinya Imada, Kotani Takenori, Chunxiao Sun, Saito Yasuyuki, Matozaki Takashi

  The 76th Annual Meeting of the Japanese Cancer Association, Sep. 2017, English, The Japanese Cancer Association, 横浜, Domestic conference

  Oral presentation


• 受容体型チロシンホスファターゼSAP-1による腸管免疫制御

  Murata Yoji, Kotani Takenori, Saito Yasuyuki, Matozaki Takashi

  なし, Jul. 2017, Japanese, なし, 淡路, Domestic conference

  Oral presentation


• Role of Src family kinases in regulation of intestinal epithelial homeostasis

  Murata Yoji, Shinya Imada, Kotani Takenori, Chunxiao Sun, Saito Yasuyuki, Matozaki Takashi

  Europhosphatase 2017:Phosphatases in cell fates and decisions, Jul. 2017, English, FASEB, Paris, France, International conference

  Poster presentation


• Regulation of intestinal immunity by the microvillus-specific protein tyrosine phosphatase SAP-1 and its substrate CEACAM20

  Kotani Takenori, Murata Yoji, Yana Supriatna, Yasuaki Kitamura, Shinya Imada, Saito Yasuyuki, Hideki Okazawa, Hiroshi Ohnishi, Matozaki Takashi

  Europhosphatase 2017:Phosphatases in cell fates and decisions, Jul. 2017, English, FASEB, Paris, France, International conference

  Poster presentation


• Blockage of CD47-SIRPαsignaling provide a novel cancer immunotherapy

  Matozaki Takashi, Murata Yoji, Tadahiko Yanagita, Saito Yasuyuki, Kotani Takenori

  Europhosphatase 2017:Phosphatases in cell fates and decisions, Jul. 2017, English, FASEB, Paris, France, International conference

  Oral presentation


• Role of SIRPα on dendritic cells in homeostatic regulation of fibroblastic reticular cells in the spleen

  Datu Respatika, Saito Yasuyuki, Ken Washio, Satomi Komori, Kotani Takenori, Murata Yoji, Matozaki Takashi

  The 45th Annual Meeting of the Japanese Society for Immunology, Dec. 2016, English, Japanese Society for Immunology, 宜野湾, Domestic conference

  Poster presentation


• Therapeutic application of anti-SIRPαantibody in cancer treatment

  Tadahiko Yanagita, Murata Yoji, Daisuke Tanaka, Saito Yasuyuki, Kotani Takenori, Komori Takahide, Matozaki Takashi

  The 12th International Conference on Protein Phosphatase, Oct. 2016, English, Japanese Association for Protein Phosphatase Research, 大阪, Domestic conference

  Poster presentation


• The anti-SIRPα antibody prevents tumor formation: a novel strategy for cancer therapy

  栁田 匡彦, Murata Yoji, 田中 大介, Saito Yasuyuki, Kotani Takenori, Komori Takahide, Matozaki Takashi

  The 75th Annual Meeting of the Japanese Cancer Association, Oct. 2016, Japanese, The Japanese Cancer Association, 横浜, Domestic conference

  Oral presentation


• Role of Src family kinases in regulatin of intestinal epithelial homeostasis

  Shinya Imada, Murata Yoji, Kotani Takenori, Masaki Hatano, Chunxiao Sun, Konno Tasuku, Saito Yasuyuki, Hideki Ohdan, Takahi Matozaki

  The 12th International Conference on Protein Phosphatase, Oct. 2016, English, Japanese Association for Protein Phosphatase Research, 大阪, Domestic conference

  Oral presentation


• Role of SIRPαin the homeostasis of fibroblastic reticular cells by dendritic cells in the spleen

  Datu Respatika, Saito Yasuyuki, Ken Washio, Satomi Komori, Kotani Takenori, Murata Yoji, Matozaki Takashi

  The 12th International Conference on Protein Phosphatase, Oct. 2016, English, Japanese Association for Protein Phosphatase Research, 大阪, Domestic conference

  Poster presentation


• 白質におけるミクログリア恒常性制御

  野津 智美, 橋本 美穂, Ruwaida Elhanbaly, 石川 達也, Saito Yasuyuki, Kotani Takenori, Murata Yoji, 深澤 有吾, Matozaki Takashi, 大西 浩史

  The 59th Annual Meeting of the Japan Neuroscience Society, Sep. 2016, Japanese, Japanese Society of Biological Psychiatry, The Japanese Society for Neurochemistry, 福岡, Domestic conference

  Poster presentation


• Role of the CD47-SIRPalpha system and its therapeutic application

  Matozaki Takashi, Murata Yoji, Saito Yasuyuki, Kotani Takenori, 栁田 匡彦, 田中 大介, 大西 浩史

  The 89th Annual Meeting of the Japanese Biochemical Society, Sep. 2016, Japanese, The Japanese Biochemical Society, 仙台, Domestic conference

  Oral presentation


• SIRPα欠損マウスはLPS投与によって誘導される低体温症が重症化する

  橋本 美穂, 野津 智美, 浦野 江里子, Saito Yasuyuki, Kotani Takenori, Murata Yoji, Matozaki Takashi, 大西 浩史

  The 59th Annual Meeting of the Japan Neuroscience Society, Sep. 2016, Japanese, Japanese Society of Biological Psychiatry, The Japanese Society for Neurochemistry, 福岡, Domestic conference

  Poster presentation


• Short chain fatty acids regulate the turnover of intestinal epithelial cells

  Konno Tasuku, Kotani Takenori, 朴 貞河, Jajar Setiawan, 北村 泰明, 今田 慎也, 臼井 佑太郎, Hatano Naoya, Shinohara Masakazu, Saito Yasuyuki, Murata Yoji, Matozaki Takashi

  The 89th Annual Meeting of the Japanese Biochemical Society, Sep. 2016, Japanese, The Japanese Biochemical Society, 仙台, Domestic conference

  Poster presentation


• Role of SIRPαon dendritic cells in homeostatic regulation of fibroblastic reticular cells in the spleen

  Datu Respatika, Saito Yasuyuki, Ken Washio, Satomi Komori, Kotani Takenori, Murata Yoji, Matozaki Takashi

  なし, Jul. 2016, English, なし, 淡路, Domestic conference

  Oral presentation


• Regulation by microbiota of the cellular life span in intestinal epithelial cells

  Kotani Takenori, 朴 貞河, 金野 祐, Jajar Setiawan, 北村 泰明, 今田 慎也, Murata Yoji, Saito Yasuyuki, Matozaki Takashi

  The 7th Annual Meeting of Japanese Association for Protein Phosphatase Research, Jan. 2016, Japanese, Japanese Association for Protein Phosphatase Research, 岡崎, Domestic conference

  Oral presentation


• Essential Role of SIRPα on Dendritic Cells in Homeostatic Regulation of Spleen Stromal Cells

  Datu Respatika, Saito Yasuyuki, Ken Washio, Satomi Komori, Kotani Takenori, Murata Yoji, Matozaki Takashi

  The 7th Annual Meeting of Japanese Association for Protein Phosphatase Research, Jan. 2016, English, Japanese Association for Protein Phosphatase Research, 岡崎, Domestic conference

  Poster presentation


• Role of C-terminal Src kinase(Csk) and Src family kinases in homeostasis of the intestinal epithelium

  今田 慎也, Murata Yoji, 北村 泰明, 朴 貞河, 畑野 正樹, 金野 祐, Kotani Takenori, Saito Yasuyuki, 大段 秀樹, Matozaki Takashi

  BMB2015 Biochemistry and Molecular Biology, Dec. 2015, Japanese, The Molecular Biology Society of Japan,The Japanese Biochemical Society, 神戸, Domestic conference

  Poster presentation


• Role of C-terminal Src kinase(Csk) and Src family kinases in homeostasis of the intestinal epithelium

  今田 慎也, Murata Yoji, 北村 泰明, 朴 貞河, 畑野 正樹, 金野 祐, Kotani Takenori, Saito Yasuyuki, 大段 秀樹, Matozaki Takashi

  BMB2015 Biochemistry and Molecular Biology, Dec. 2015, Japanese, The Molecular Biology Society of Japan,The Japanese Biochemical Society, 神戸, Domestic conference

  Oral presentation


• Importance of commensal bacteria and short-chain fatty acids for the growth and migration of intestinal epithelial cells

  Kotani Takenori, 朴 貞河, 北村 泰明, 今田 慎也, Murata Yoji, Saito Yasuyuki, Matozaki Takashi

  BMB2015 Biochemistry and Molecular Biology, Dec. 2015, Japanese, The Molecular Biology Society of Japan,The Japanese Biochemical Society, 神戸, Domestic conference

  Poster presentation


• Regulation of intestinal immunity by R3-subtype receptor-type protein tyrosine phosphatase SAP-1

  Murata Yoji, Kotani Takenori, 齋藤 泰之, 岡澤 秀樹, 大西 浩史, Matozaki Takashi

  BMB2015 Biochemistry and Molecular Biology, Dec. 2015, Japanese, The Molecular Biology Society of Japan,The Japanese Biochemical Society, 神戸, Domestic conference

  Public symposium


• Role of C-terminal Src kinase(Csk) and Src family kinases in homeostasis of the intestinal epithelium

  今田 慎也, Murata Yoji, 北村 泰明, 朴 貞河, Kotani Takenori, Saito Yasuyuki, 大段 秀樹, Matozaki Takashi

  The 74th Annual Meeting of the Japanese Cancer Association, Oct. 2015, Japanese, The Japanese Cancer Association, 名古屋, Domestic conference

  Oral presentation


• Importance of commensal bacteria and short-chain fatty acids for the growth and migration of intestinal epithelial cells

  Kotani Takenori, 朴 貞河, 北村 泰明, 今田 慎也, Murata Yoji, Saito Yasuyuki, Matozaki Takashi

  The 74th Annual Meeting of the Japanese Cancer Association, Oct. 2015, Japanese, The Japanese Cancer Association, 名古屋, Domestic conference

  Oral presentation


• SAP-1 and CEACAM20 protect against colitis in the intestinal epithelium

  Murata Yoji, Kotani Takenori, Yana Supriatna, Yasuaki Kitamura, Shinya Imada, Saito Yasuyuki, Hideki Okazawa, Hiroshi Ohnishi, Matozaki Takashi

  2015 FASEB Science Research Conferences, Aug. 2015, English, FASEB, SteamboatSprings, USA, International conference

  Poster presentation


• Role of C-terminal Src kinase(Csk) and Src family kinases in homeostasis of the intestinal epithelium

  Shinya Imada, Murata Yoji, Yasuaki Kitamura, Jung-ha Park, Masaki Hatano, Kotani Takenori, Saito Yasuyuki, Hideki Ohdan, Matozaki Takashi

  2015 FASEB Science Research Conferences, Aug. 2015, English, FASEB, SteamboatSprings, USA, International conference

  Poster presentation


• Regulation of intestinal immunity by the microvillus-specific protein tyrosine phosphatase SAP-1 and CEACAM20

  Murata Yoji, Kotani Takenori, Yana Supriatna, 北村 泰明, 今田 慎也, Saito Yasuyuki, 大西 浩史, Matozaki Takashi

  The 67th Annual Meeting of the Japan Society for Cell Biology, Jul. 2015, Japanese, The Japan Society for Cell Biology, 東京, Domestic conference

  Poster presentation


• Regulation by microbiota of the cellular life span in intestinal epithelial cells

  Kotani Takenori, 朴 貞河, 北村 泰明, 今田 慎也, Murata Yoji, Saito Yasuyuki, Matozaki Takashi

  The 67th Annual Meeting of the Japan Society for Cell Biology, Jul. 2015, Japanese, The Japan Society for Cell Biology, 東京, Domestic conference

  Poster presentation


• Behavioral analysis of forebrain neuron-specific Shp2 conditional knockout mice

  Hiroshi Ohnishi, Shinya Kusakari, Miho Hashimoto, Shuya Ishikawa, Eriko Urano, Kotani Takenori, Murata Yoji, Matozaki Takashi

  The 38th Annual Meeting of the Japan Neuroscience Society, Jul. 2015, English, The Japan Neuroscience Society, 神戸, Domestic conference

  Poster presentation


• 細胞間シグナル伝達システムCD47-SIRPα系のがん治療への応用

  栁田 匡彦, Murata Yoji, 田中 大介, Kotani Takenori, Saito Yasuyuki, Komori Takahide, Matozaki Takashi

  なし, Jul. 2015, Japanese, なし, 箱根, Domestic conference

  Oral presentation


• Analysis of cell-cell interaction signal that regulates microglial homeostasis

  Miho Sato-Hashimoto, Tomomi Nozu, Eriko Urano, Saito Yasuyuki, Kotani Takenori, Murata Yoji, Matozaki Takashi, Hiroshi Ohnishi

  The 38th Annual Meeting of the Japan Neuroscience Society, Jul. 2015, English, The Japan Neuroscience Society, 神戸, Domestic conference

  Poster presentation


• Role of the protein tyrosine phosphatase Shp2 in homeostasis of the intestinal epithelium

  Kotani Takenori, Hironari Yamashita, Jung-ha Park, Murata Yoji, Hideki Okazawa, Hiroshi Ohnishi, Ku Yonson, Matozaki Takashi

  The 5th International Symposium on Carcinogenic SpiralInfection, Immunity, and Cancer, Feb. 2015, English, Carcinogenic Spiral, 神戸, International conference

  Poster presentation


• Essential role og SIRPα on dendritic dells in organization and homeostasis of the spleen

  Ken Washio, Kotani Takenori, Datu Respatika, Murata Yoji, Saito Yasuyuki, Hideki Okazawa, Hiroshi Ohnishi, Fukunaga Atsushi, Nishigori Chikako, Matozaki Takashi

  The 43rd Annual Meeting of The Japanese Society for Immunology, Dec. 2014, English, Japanese Society for Immunology, 京都, Domestic conference

  Oral presentation


• Essential role of SIRP on dendritic cells in organization and homeostasis of the spleen

  Ken Washio, Kotani Takenori, Respatika Datu, Murata Yoji, Saito Yasuyuki, Yoriaki Kaneko, Hideki Okazawa, Hiroshi Ohnishi, Fukunaga Atsushi, Nishigori Chikako, Matozaki Takashi

  第43回日本免疫学会学術集会, Dec. 2014, English, 日本免疫学会, 大阪, Domestic conference

  Oral presentation


• Regulation by CD-SIRPα signaling of hematopoietic and immune systems

  Kotani Takenori, 鷲尾 健, ダトゥ レスパティカ, Murata Yoji, Saito Yasuyuki, Matozaki Takashi

  The 37th Annual Meeting of the Molecular Biology Society of Japan, Nov. 2014, Japanese, The Molecular Biology Society of Japan, 横浜, Domestic conference

  Public symposium


• Shear stress-induced redistribution of VE-PTP in endothelial cell and its role in cell elongation

  Murata Yoji, Isnainiasih Mantilidewi Kemala, Kotani Takenori, Hiroshi Ohnishi, Matozaki Takashi

  11th International Conference on Protein PhosphataseProtein Phosphatases in Health and Diseases, Nov. 2014, English, Japanese Association for Protein Phosphatase Research, 仙台, International conference

  Oral presentation


• Roles of protein throsine phosphatases in homeostasis of intestinal epithelium

  Matozaki Takashi, Murata Yoji, Kotani Takenori, Saito Yasuyuki

  11th International Conference on Protein PhosphataseProtein Phosphatases in Health and Diseases, Nov. 2014, English, Japanese Association for Protein Phosphatase Research, 仙台, International conference

  Oral presentation


• Regulation by the intestinal microflora of Carcinoembryonic antigen-related cell adhesion molecule (CEACAM) 20 expression in intestinal epithelial cells

  Yasuaki Kitamura, Murata Yoji, Jung-ha Park, Kotani Takenori, Azuma Takeshi, Matozaki Takashi

  11th International Conference on Protein PhosphataseProtein Phosphatases in Health and Diseases, Nov. 2014, English, Japanese Association for Protein Phosphatase Research, 仙台, International conference

  Poster presentation


• Regulation by microbiota of intestinal epithelium turnover

  Jung-ha Park, Kotani Takenori, Yasuaki Kitamura, Murata Yoji, Matozaki Takashi

  The 37th Annual Meeting of the Molecular Biology Society of Japan, Nov. 2014, English, The Molecular Biology Society of Japan, 横浜, Domestic conference

  Poster presentation


• Functinal analysis of protein tyrosine phosphatase Shp2 in post-mitotic neurons

  Shinya Kusakari, Fumihito Saitow, Miho Hashimoato, Yasunori Matsuzaki, Kotani Takenori, Murata Yoji, Hirokazu Hirai, Hidenori Suzuki, Matozaki Takashi, Hiroshi Ohnishi

  11th International Conference on Protein PhosphataseProtein Phosphatases in Health and Diseases, Nov. 2014, English, Japanese Association for Protein Phosphatase Research, 仙台, International conference

  Oral presentation


• Roles of the protein tyrosine phosphatase Shp2 in maintaining homeostasis of the intestinal epithelium

  Kotani Takenori, 山下 博成, Murata Yoji, 岡澤 秀樹, 大西 浩史, Ku Yonson, Matozaki Takashi

  The 87th Annual Meeting of the Japanese Biochemical Society, Oct. 2014, Japanese, The Japanese Biochemical Society, 京都, Domestic conference

  Oral presentation


• Expression of CEACAM20 in intestine epithelial cells and regulation of its expression by intestinal microflora

  北村 泰明, Murata Yoji, Bak Jeongha, 今田 慎也, Kotani Takenori, 岡澤 秀樹, Azuma Takeshi, Matozaki Takashi

  The 87th Annual Meeting of the Japanese Biochemical Society, Oct. 2014, Japanese, The Japanese Biochemical Society, 京都, Domestic conference

  Oral presentation


• Expression of CEACAM20 in intestinal epithelial cells and regulation of its expression by intestinal microflora

  Yasuaki Kitamura, Murata Yoji, Jung-ha Park, Shinya Imada, Kotani Takenori, Hideki Okazawa, Azuma Takeshi, Matozaki Takashi

  第87回 日本生化学会大会, Oct. 2014, Japanese, 日本生化学会, 京都, Domestic conference

  Poster presentation


• Roles of the protein tyrosine phosphatase VE-PTP in endothelial Cells of tumor vessels and normal their counterparts

  Murata Yoji, Isnainiasih Mantilidewi Kemala, Munemasa Mori, Kotani Takenori, Shinya Kusakari, Hiroshi Ohnishi, Matozaki Takashi

  The 73rd Annual Meeting of the Japanese Cancer Association, Sep. 2014, English, The Japanese Cancer Association, 横浜, Domestic conference

  Oral presentation


• Shp2ホスファターゼはRas活性化を介して腸上皮の恒常性と腸炎発症を制御する

  Matozaki Takashi, 山下 博成, Kotani Takenori, Bak Jeongha, Murata Yoji, 岡澤 秀樹, 大西 浩史, Ku Yonson

  第73回日本癌学会学術総会, Sep. 2014, English, がん研究会, 神奈川, Domestic conference

  Oral presentation


• Regulation of microglial homeostasis through cell-cell interaction signal

  Miho Sato-Hashimoto, Yuriko Hayashi, Shinya Kusakari, Kotani Takenori, Murata Yoji, Matozaki Takashi, Hiroshi Ohnishi

  Neuroscience 2014The 37th Annual Meeting of the Japan Neuroscience Society, Sep. 2014, English, The Japan Neuroscience Society, 横浜, Domestic conference

  Poster presentation


• Functional Analysis of protein tyrosine phosphatase Shp2 in the adult forebrain neurons

  Hiroshi Ohnishi, Shinya Kusakari, Fumihito Saitow, Miho Hashimoto, Yasunori Matsuzaki, Kotani Takenori, Murata Yoji, Hirokazu Hirai, Hidenori Suzuki, Matozaki Takashi

  Neuroscience 2014The 37th Annual Meeting of the Japan Neuroscience Society, Sep. 2014, English, The Japan Neuroscience Society, 横浜, Domestic conference

  Oral presentation


• 腸管におけるCDACAM20の発現と腸内細菌叢を介した発現制御

  北村 泰明, Murata Yoji, Bak Jeongha, Kotani Takenori, 岡澤 秀樹, Azuma Takeshi, Matozaki Takashi

  なし, Jul. 2014, Japanese, なし, 岐阜, Domestic conference

  Oral presentation


• 樹状細胞及び二次リンパ組織の恒常維持におけるSIRPαの役割

  鷲尾 健, Kotani Takenori, ダトゥ レスパティカ, Murata Yoji, Saito Yasuyuki, 金子 和光, 岡澤 秀樹, 大西 浩史, Fukunaga Atsushi, Nishigori Chikako, Matozaki Takashi

  なし, Jul. 2014, Japanese, なし, 岐阜, Domestic conference

  Oral presentation


• Roles of the protein tyrosine phosphatase Shp2 in maintaining homeostasis of the intestinal epithelium

  Kotani Takenori, 山下 博成, Murata Yoji, 岡澤 秀樹, 大西 浩史, Ku Yonson, Matozaki Takashi

  The 66th Annual Meeting of the Japan Society for Cell Biology, Jun. 2014, Japanese, The Japan Society for Cell Biology, 奈良, Domestic conference

  Oral presentation


• Shear Stress-induced Redistribution of Vascular Endothelial-Protein-tyrosine Phosphatase (VE-PTP) in Endothelial Cells and Its Role in Cell Elongation.

  Murata Yoji, ケマラ イスナイニアシ, マンティリデヴィ, 森 宗昌, Kotani Takenori, 大坪 千裕, 草苅 伸也, 大西 浩史, Matozaki Takashi

  The 66th Annual Meeting of the Japan Society for Cell Biology, Jun. 2014, Japanese, The Japan Society for Cell Biology, 奈良, Domestic conference

  Oral presentation


• Shear Stress-induced Redistribution of Vascular Endothelial-Protein-tyrosine Phosphatase (VE-PTP) in Endothelial Cells and Its Role in Cell Elongation.

  Isnainiasih Mantilidewi Kemala, Murata Yoji, Munemasa Mori, Kotani Takenori, Shinya Kusakari, Hideki Okazawa, Hiroshi Ohnishi, Matozaki Takashi

  The 18th International Vascular Biology Meeting（IVBM2014）, Apr. 2014, English, International Vascular Biology Meeting, 京都, International conference

  Poster presentation


• SIRPα-CD47 system regulates the homeostasis of dendritic cells in lymphoid tissues

  Ken Washio, Kotani Takenori, Datu Respatika, Murata Yoji, Hideki Okazawa, Hiroshi Ohnishi, Fukunaga Atsushi, Nishigori Chikako, Matozaki Takashi

  University of Washington-Kobe University Symposium on Membrane biology, Mar. 2014, English, University of Washington-Kobe University, Seattle, USA, International conference

  Poster presentation


• SIRPα-CD47 system regulates the homeostasisof dendritic cells in lymphoid tissues

  Ken Washio, Kotani Takenori, Datu Respatika, Murata Yoji, Hideki Okazawa, Hiroshi Ohnishi, Fukunaga Atsushi, Nishigori Chikako, Matozaki Takashi

  The 2nd University of Washington-Kobe University Joint Symposium on Membrane Biology, Mar. 2014, English, University of Washington, Seattle, USA, International conference

  Poster presentation


• Role of VE-PTP in shear stress responses of endothelial cells

  Isnainiasih Mantilidewi Kemala, Murata Yoji, Munemasa Mori, Kotani Takenori, Shinya Kusakari, Hideki Okazawa, Hiroshi Ohnishi, Matozaki Takashi

  University of Washington-Kobe University Symposium on Membrane biology, Mar. 2014, English, University of Washington-Kobe University, Seattle, USA, International conference

  Poster presentation


• 腸上皮の恒常性維持におけるチロシンホスファターゼShp2の役割

  Kotani Takenori, 山下 博成, 朴 貞河, Murata Yoji, 岡澤 秀樹, 大西 浩史, Ku Yonson, Matozaki Takashi

  The 6th Annual Meeting of Japanese Association for Protein Phosphatase Research, Feb. 2014, Japanese, Japanese Association for Protein Phosphatase Research, 三重, Domestic conference

  Oral presentation


• 樹状細胞及び二次リンパ組織の恒常性維持におけるSIRPαの役割

  鷲尾 健, Kotani Takenori, レスパティカ ダトゥ, Murata Yoji, 岡澤 秀樹, 大西 浩史, Fukunaga Atsushi, Nishigori Chikako, Matozaki Takashi

  The 6th Annual Meeting of Japanese Association for Protein Phosphatase Research, Feb. 2014, Japanese, Japanese Association for Protein Phosphatase Research, 三重, Domestic conference

  Oral presentation


• 細胞質型チロシンホスファターゼShp2による脳機能制御

  草苅 伸也, 齋藤 文仁, 橋本 美穂, 柴崎 貢志, 吾郷 由紀夫, Kotani Takenori, Murata Yoji, 松田 敏夫, Benjamin G. Neel, Matozaki Takashi, 大西 浩史

  The 6th Annual Meeting of Japanese Association for Protein Phosphatase Research, Feb. 2014, Japanese, Japanese Association for Protein Phosphatase Research, 三重, Domestic conference

  Oral presentation


• SIRPα-CD47 system regulates the homeostasis of dendritic cells in lymphoid tissues

  WASHIO Ken, Kotani Takenori, RESPATIKA Datu, Murata Yoji, OKAZAWA Hideki, OHNISHI Hiroshi, Fukunaga Atsushi, Nishigori Chikako, Matozaki Takashi

  Annual Meeting of the Japanese Society for Immunology, 2013, Dec. 2013, English, Japanese Society for Immunology, 千葉, Domestic conference

  Poster presentation


• R3 受容体型チロシンホスファターゼの発現、局在と生理機能

  Matozaki Takashi, Murata Yoji, Kotani Takenori, Yana Supriatna, 森 宗昌, 岡澤 秀樹, 大西 浩史

  The 36th Annual Meeting of the Molecular Biology Society of Japan, Dec. 2013, Japanese, The Molecular Biology Society of Japan, 兵庫, Domestic conference

  [Invited]

  Nominated symposium


• Roles of the protein tyrosine phosphatase Shp2 in maintaining 213 homeostasis of the intestinal epithelium

  山下 博成, Kotani Takenori, Murata Yoji, 岡澤 秀樹, 大西 浩史, Ku Yonson, Matozaki Takashi

  The 72rd Annual Meeting of the Japanese Cancer Association, Oct. 2013, Japanese, The Japanese Cancer Association, 神奈川, Domestic conference

  Oral presentation


• Regulation of intestinal immunity by the protein tyrosine phosphatase SAP-1

  Murata Yoji, Kotani Takenori, Supriatna Yana, 森 宗昌, 草苅 伸也, 岡澤 秀樹, 大西 浩史, Matozaki Takashi

  The 72rd Annual Meeting of the Japanese Cancer Association, Oct. 2013, Japanese, The Japanese Cancer Association, 神奈川, Domestic conference

  Oral presentation


• Roles of protein tyrosine phosphatases Shp2 in homeostasis of intestinal epithelium

  Kotani Takenori

  第1回神緑会ﾔﾝｸﾞｲﾝｳﾞｪｽﾃｨｹﾞｰﾀｰｱﾜｰﾄﾞ発表会, Oct. 2013, English, 神緑会, 神戸, Domestic conference

  Poster presentation


• Roles of the protein tyrosine phosphatase Shp2 in maintaining homeostasis of the intestinal epithelium

  Kotani Takenori, 山下 博成, Murata Yoji, 岡澤 秀樹, 大西 浩史, Ku Yonson, Matozaki Takashi

  The 86th Annual Meeting of the Japanese Biochemical Society, Sep. 2013, Japanese, The Japanese Biochemical Society, 神奈川, Domestic conference

  Oral presentation


• Molecular basis for regulation of the life span of intestinal epithelial cell

  Matozaki Takashi, Kotani Takenori, Murata Yoji

  The 86th Annual Meeting of the Japanese Biochemical Society, Sep. 2013, Japanese, The Japanese Biochemical Society, 神奈川, Domestic conference

  [Invited]

  Nominated symposium


• 細胞質型チロシンホスファターゼShp2の成熟脳における機能解析

  草苅 伸也, 橋本 美穂, 柴崎 貢志, 吾郷 由希夫, 松田 敏夫, Benjamin G. Neel, Kotani Takenori, Murata Yoji, Matozaki Takashi, 大西 浩史

  The 60th Annual Meeting of KITAKANTO Medical Society, Sep. 2013, Japanese, KITAKANTO Medical Society, 群馬, Domestic conference

  Poster presentation


• Role of VE-PTP in shear stress responses of endothelial cells

  Isnainiasih Mantilidewi Kemala, Murata Yoji, Munemasa Mori, Kotani Takenori, Shinya Kusakari, Hideki Okazawa, Hiroshi Ohnishi, Matozaki Takashi

  The 86th Annual Meeting of the Japanese Biochemical Society, Sep. 2013, English, The Japanese Biochemical Society, 神奈川, Domestic conference

  Oral presentation


• SIRPαによる脾臓T細胞の恒常性の調節

  橋本 美穂, 齊藤 泰之, 金子 哲也, 大西 浩史, 草苅 伸也, Kotani Takenori, Murata Yoji, 岡澤 秀樹, Matozaki Takashi

  The 60th Annual Meeting of KITAKANTO Medical Society, Sep. 2013, Japanese, KITAKANTO Medical Society, 群馬, Domestic conference

  Poster presentation


• Roles of the protein tyrosine phosphatase Shp2 in homeostasis of the intestinal epithelium

  Kotani Takenori, 山下 博成, Murata Yoji, 岡澤 秀樹, 大西 浩史, Matozaki Takashi

  2013 FASEB Science Research Conferences, Aug. 2013, English, FASEB, SteambatSprings（Colorad）, USA, International conference

  Poster presentation


• Microbiota modulates the turnover of intestinal epithelial cells

  Jung-ha Park, Kotani Takenori, Murata Yoji, Matozaki Takashi

  2013 FASEB Science Research Conferences, Aug. 2013, English, FASEB, SteambatSprings（Colorad）, USA, International conference

  Poster presentation


• 腸上皮の恒常性維持におけるチロシンホスファターゼShp2の役割

  Kotani Takenori, 山下 博成, Murata Yoji, 岡澤 秀樹, 大西 浩史, Ku Yonson, Matozaki Takashi

  第12回生体機能研究会, Jul. 2013, Japanese, 生体機能研究会, 群馬, Domestic conference

  Oral presentation


• 成熟腸上皮細胞の寿命決定の分子基盤

  Matozaki Takashi, 朴 貞河, Kotani Takenori

  第12回生体機能研究会, Jul. 2013, Japanese, 生体機能研究会, 群馬, Domestic conference

  Oral presentation


• Ｈｙｐｔｈｅｒｍｉａ−ｄｅｐｅｎｄｅｎｔ ａｎｄ −ｉｎｄｅｐｅｎｄｅｎｔ effects of forced swim on the phosphorylation satates of signaling molecules in mouse hippocampus

  橋本(佐藤) 美穂, 林 由里子, 草苅 伸也, 浦野 江里子, 滋野 雅大, 関島 恒夫, Kotani Takenori, Murata Yoji, 村上 博和, Matozaki Takashi, 大西 浩史

  The 36th annual Meeting of the Japan Neuroscience SocietyThe 56th Annual Meeting of Japanese Society for NeurochemistryThe 23rd Annual Conference of Japanese Neural Network Society, Jun. 2013, English, The Japan Neuroscience Society, 京都, Domestic conference

  Poster presentation


• Functional Analysis of A non-receptor type protein tyrosine phosphatase Shp2 in the adult brain

  草苅 伸也, 齋藤 文仁, 橋本(佐藤) 美穂, 柴崎 貢志, 吾郷 由希夫, 松田 敏夫, Neel BG, Kotani Takenori, Murata Yoji, Matozaki Takashi, 大西 浩史

  The 36th annual Meeting of the Japan Neuroscience SocietyThe 56th Annual Meeting of Japanese Society for NeurochemistryThe 23rd Annual Conference of Japanese Neural Network Society, Jun. 2013, English, The Japan Neuroscience Society, 京都, Domestic conference

  Poster presentation


• Tyrosine phosphorylation of CEACAM20 and its functional roles

  エドウィン ウィジャント, ダニウィジャヤ, Murata Yoji, Kotani Takenori, 大西 浩史, Matozaki Takashi

  10th International Conference on Protein Phosphatase Protein Phosphatases and Diseases, Feb. 2013, English, Japanese Association for Protein Phosphatase Research, 東京, CEACAM20 is an immunoglobulin-superfamily transmembrane protein that contains the ITAM-like motif in its cytoplasmic region. We have recently found that CEACAM20 is a putative substrate of SAP-1, a receptor type tyrosine phosphatase, in mouse intestinal epithelial cells. However, tyrosine phosphorylation of CEACAM20 and its physiological significance remain largely unknown. Her, International conference

  Poster presentation


• Hypothermia-induced tyrosine phosphorylation of SIRPα in the brain

  草苅 伸也, 丸山 登士, 橋本 美穂, 林 由里子, 浦野 江里子, 草苅 百合子, Kotani Takenori, Murata Yoji, 岡澤 秀樹, Per-Arne Oldenborg, Matozaki Takashi, 大西浩史

  10th International Conference on Protein Phosphatase Protein Phosphatases and Diseases, Feb. 2013, English, Japanese Association for Protein Phosphatase Research, 東京, Protein tyrosine phosphatase Shp2 expresses ubiquitously, and promotes the activation of the Ras-MAPK cascade by growth factor and cytokine stimulation. Shp2 regulates proliferation and differentiation via Ras/MAPK cascade, and Shp2 deficient mice were embryonic lethal. Shp2 also is abundantly expressed in the adult brain, and Shp2 activity promotes differentiation of neuronal, International conference

  Poster presentation


• Dendritic cell-specific ablation of the protein tyrosine phosphatase Shp1 promotes Th1 cell diffierentiation and induces autoimmunity

  Kotani Takenori, 金子 哲也, 齋藤 泰之, 橋本 美穂, 草苅 伸也, 金子 和光, Murata Yoji, 大西 浩史, 高岸 憲二, Matozaki Takashi

  10th International Conference on Protein Phosphatase Protein Phosphatases and Diseases, Feb. 2013, English, Japanese Association for Protein Phosphatase Research, 東京, Dendritic cells (DCs) promote immune responses to foreign antigens (Ags) and immune tolerance to self-Ags. Deregulation of DCs is implicated in autoimmunity, but the molecules that regulate DCs to protect against autoimmunity have remained unknown. In this study, we show that mice lacking the protein tyrosine phosphatase Shp1 specifically in DCs develop splenomegaly associated, International conference

  Poster presentation


• Signal regulatory protein alpha regulates the homeostasis of T lymphocytes in the spleen.

  橋本(佐藤) 美穂, 齊藤 泰之, 金子 哲也, 大西 浩史, 草苅 伸也, Kotani Takenori, Murata Yoji, 岡澤 秀樹, Matozaki Takashi

  The 85th Annual Meeting of the Japanese Biochemical Society, Dec. 2012, Japanese, The Japanese Biochemical Society, 福岡, SIRPα（Signal regulatory proteinα）は、樹状細胞やマクロファージに発現する膜タンパク質であり、その細胞外領域のリガンドである膜タンパク質CD47と相互作用し、細胞間相互作用シグナルCD47-SIRPα系を構成する。今回我々は、SIRPαノックアウト（KO）マウスの脾臓において、白脾髄の縮小と、CD4陽性T細胞数の減少を見出した。また、脾臓Ｔ細胞領域のストローマ細胞が産生するケモカインであるCCL19およびCCL21、サイトカインであるIL-7の遺伝子発現量の減少も見出した。同様のフェノタイプは、SIRPαのリガンドであるCD47のKOマウスでも認められた。これらの結果から、CD47-SIRPα系が、T細胞の恒常性維持に関与する可能性が考えられた。さらに、SIRPα KOと野生型マウスの間で作製した骨髄キメラマウスを解析, Domestic conference

  Poster presentation


• Hypothermia-induced tyrosine phosphorylation of SIRPa in the brain

  草苅 伸也, 丸山 登士, 橋本 美穂, 林 由里子, 草苅 百合子, Kotani Takenori, Murata Yoji, 岡澤 秀樹, 岸 章治, Matozaki Takashi, 大西浩史

  11th biennial meeting of APSN/55th Meeting of JSN, Oct. 2012, Japanese, The Japanese Society for Neurochemistry, The Asian Pacific Society of Nephrology, 兵庫, Signal regulatory protein a (SIRPa also known as SHPS-1, BIT, or P84) is a neuronal membrane protein that has three immunoglobulin-like domains in its extracellular region and tyrosine phosphorylation sites in the cytoplasmic region. Tyrosine phosphorylated SIRPa binds and activates protein tyrosine phosphatase Shp2. Previously, we found that SIRP a undergoes tyrosine phosphory, International conference

  Oral presentation


• Importance of The Protein Tyrosine Phosphatase Shp1 In Dendritic Cells for Prevention of Th1 Cell Differentiation And Autoimmunity: A Potential Target for the Therapy

  金子 哲也, 齋藤 泰之, Kotani Takenori, 大西 浩史, Murata Yoji, 米本 由木夫, 岡邨 興一, Matozaki Takashi, 高岸 憲二

  欧州リウマチ学会議2012, Jun. 2012, English, The European League Against Rheumatism, Berlin, Germany, Background: Dendritic cells (DCs) are professional antigen-presenting cells crucial for initiating immune response to pathogens as well as for maintaining immune tolerance to self-antigens. Dysregulation of the function of DCs is thought to cause abnormal immune responses to self-antigens, which in turn cause autoimmunity. Src homology 2 domaincontaining protein tyrosine phosph, International conference

  Oral presentation


• 腸微絨毛特異的な発現を示す受容体型チロシンホスファターゼSAP-1による腸管免疫制御

  Kotani Takenori, Murata Yoji, ヤナ スプリアトゥナ, 岡澤 秀樹, 大西 浩史, エドウィン ダニヴィジャヤ, 草苅 伸也, 定方 久延, 大川 克也, Matozaki Takashi

  第5回プロテインホスファターゼ研究会 学術集会 大阪, Jan. 2012, Japanese, 日本プロテインホスファターゼ研究会, 吹田市, Domestic conference

  Oral presentation


• 成熟脳におけるShp2の生理機能解析

  草苅 伸也, Kotani Takenori, 橋本 美穂, Murata Yoji, 岡澤 秀樹, 吾郷 由紀夫, 松田 敏夫, ベンジャミン G ニール, Matozaki Takashi, 大西 浩史

  第5回プロテインホスファターゼ研究会 学術集会 大阪, Jan. 2012, Japanese, 日本プロテインホスファターゼ研究会, 吹田市, Domestic conference

  Oral presentation


• 蛋白質チロシンホスファターゼShp2の成熟脳における機能解析

  草苅 伸也, Kotani Takenori, 橋本 美穂, 林 由里子, 草苅 百合子, Murata Yoji, 岡澤 秀樹, Matozaki Takashi, 大西 浩史

  第2回国際放射線神経生物学会大会, Dec. 2011, Japanese, International Society of Radiation Neurobiology, 前橋市, International conference

  Poster presentation


• Dendritic cell-specific depletion of protein tyrosine phosphatase Shp1 promotes Th1 differentiation causes autoimmunity

  金子 哲也, 齊藤 泰之, 大西 浩史, 橋本 美穂, Kotani Takenori, Murata Yoji, 岡澤 秀樹, 高岸 憲二, Matozaki Takashi

  第40回日本免疫学会学術集会, Nov. 2011, English, 日本免疫学会, 千葉市（幕張）, Domestic conference

  Poster presentation


• 腸微絨毛特異的な発現を示す受容体型チロシンキナーゼSAP-1とIL-10による炎症性腸疾患の制御

  Kotani Takenori, Murata Yoji, Yana Supriatna, 岡澤 秀樹, 大西 浩史, Edwin Daniwijaya, 定方 久延, 草苅 伸也, 齊藤 泰之, 大川 克也, Matozaki Takashi

  BMB2010第33回日本分子生物学会年会第85回日本生化学会大会合同年会, Dec. 2010, Japanese, 日本分子生物学会・日本生化学会, 神戸, 日本, Domestic conference

  Poster presentation


• 血管内皮細胞のシェアストレス刺激応答におけるVE-PTPの機能解析

  Murata Yoji, 森 宗昌, Kotani Takenori, 草苅 伸也, 岡澤 秀樹, 齊藤 泰之, 大西 浩史, Matozaki Takashi

  BMB2010第33回日本分子生物学会年会第84回日本生化学会大会合同年会, Dec. 2010, Japanese, 日本分子生物学会・日本生化学会, 神戸, 日本, Domestic conference

  Oral presentation


• Promotion of cell spreading and migration by vascular endothelial-protein tyrosine phosphatase (VE-PTP) in cooperation with integrin

  森 宗昌, Murata Yoji, Kotani Takenori, 草苅 伸也, 大西 浩史, 齊藤 泰之, 岡澤 秀樹, 森 昌朋, Matozaki Takashi

  FASEB Summer Research Conferences"Protein Phosphatases", Jul. 2010, English, 米国連邦実験生物学会, コロラド, アメリカ, International conference

  Poster presentation


• Expression of PTPRO in the interneurons of adult mouse olfactory bulb

  Kotani Takenori, Murata Yoji, 大西 浩史, 森 宗昌, 草苅 伸也, 齊藤 泰之, 岡澤 秀樹, John L. Bixby, Matozaki Takashi

  FASEB Summer Research Conferences"Protein Phosphatases", Jul. 2010, English, 米国連邦実験生物学会, コロラド, アメリカ, International conference

  Poster presentation

• 腸上皮細胞の寿命制御に関わる分子基盤の解明

  小谷 武徳

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Kobe University, 01 Apr. 2020 - 31 Mar. 2023


• 腸上皮細胞の寿命を制御する分子基盤の解明

  小谷 武徳

  学術研究助成基金助成金／基盤研究(C), Apr. 2016 - Mar. 2019, Principal investigator

  Competitive research funding


• Molecular basis of the CD47-SIRP alpha signaling system in the regulation of physiological function

  Matozaki Takashi, OHNISHI Hiroshi, MURATA Yoji, SAITO Yasuyuki, KOTANI Takenori

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Kobe University, 01 Apr. 2014 - 31 Mar. 2017

  We have here investigated the molecular basis of the CD47-SIRPα signaling system in the regulation of physiological function in the central nervous system (CNS) as well as in the immune system. In the CNS, we have shown that the protein tyrosine phosphatase Shp-2, which is a downstream molecule of SIRPα, regulates synaptic functions and thereby modulates learning and locomotor activity. In the immune system, we have shown that SIRPα is essential for the maintenance of dendritic cell homeostasis in the secondary lymphoid organs and skin. Furthermore, we have found that anti-SIRPα antibodies appear to be a potential new tool for cancer immunotherapy.


• 細胞間シグナル伝達システムCD47-SIRPα系による免疫制御機構の解析

  小谷 武徳

  学術研究助成基金助成金／若手研究(B), Apr. 2014 - Mar. 2016, Principal investigator

  Competitive research funding


• チロシンホスファターゼＳｈｐ－２を介した発がんスパイラル機構の解明

  小谷 武徳

  科学研究費補助金／新学術領域研究, Apr. 2013 - Mar. 2015, Principal investigator

  Competitive research funding


• 受容体型チロシンホスファターゼＳＡＰ-1による腸管免疫制御機構

  小谷 武徳

  科学研究費補助金／若手研究(B), Apr. 2011 - Mar. 2013, Principal investigator

  Competitive research funding