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KUMABE Yohei
University Hospital / Orthopedic Surgery
Assistant Professor

Researcher basic information

■ Research Keyword
  • 骨関節感染症
  • 難治性骨折
  • 骨折
■ Research Areas
  • Life sciences / Orthopedics

Research activity information

■ Paper
  • Ryota Nishida, Yohei Kumabe, Kenichi Sawauchi, Yutaka Matsumiya, Tomoaki Fukui, Hyuma Kondo, Takahiro Niikura, Ryosuke Kuroda, Keisuke Oe
    Jul. 2026, Injury
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Keisuke Yoshida, Yohei Kumabe, Keisuke Oe, Tomoaki Fukui, Ryosuke Kuroda
    Pediatric femoral pseudarthrosis is rare, and particularly in cases with significant rotational deformity, insights regarding treatment guidelines and the safety of surgical techniques are limited. In this report, we present a case of pediatric femoral pseudarthrosis that achieved good bone union and functional recovery through one-stage rotational correction combined with a wake-up test to avoid neurological complications. A 12-year-old boy, who had undergone surgical fixation for a right diaphyseal femur fracture at a previous hospital, was diagnosed with pseudarthrosis with 56° of external rotation deformity. At our institution, after freshening the pseudarthrosis site, we performed a one-stage rotational correction of 56° using a Hoffman-type external fixator while evaluating the nerve injury with a wake-up test. Neither intraoperative nor postoperative nerve impairment was observed, and synostosis was achieved at 13 postoperative weeks. In the correction of rotational deformities, particularly in cases with large correction angles, there is a risk of nerve damage due to traction or compression of peripheral nerves. The wake-up test is a simple method for directly assessing nerve function, even under conditions with limitations on monitoring equipment, and it is useful for the early detection and prevention of neurological complications. In this case, a 56° rotational correction for pediatric femoral pseudarthrosis was performed in a one-stage procedure, and safe surgery was accomplished through intraoperative neurological assessment using the wake-up test. This suggests that the wake-up test can be a valuable auxiliary evaluation method even in situations where intraoperative nerve monitoring is limited.
    Nov. 2025, Cureus, 17(11) (11), e96867, English, International magazine
    Scientific journal

  • Kohei Motono, Tomoyuki Matsumoto, Keisuke Oe, Masanori Tsubosaka, Naoki Nakano, Tomoaki Fukui, Yohei Kumabe, Ryosuke Kuroda
    BACKGROUND Continuous local antibiotic perfusion (CLAP) delivers gentamicin continuously via a bone marrow needle and double-lumen tube in the infected area, taking advantage of the concentration-dependent bactericidal activity of gentamicin, and allowing even resistant bacteria to be inhibited at high local concentrations. CLAP has shown good outcomes in bone and soft-tissue infections. However, reports on its use for periprosthetic joint infection (PJI) after total knee arthroplasty are limited. Moreover, bilateral simultaneous methicillin-resistant Staphylococcus aureus (MRSA) infection following total knee arthroplasty is rare. We report the successful CLAP treatment of simultaneous bilateral MRSA infection after total knee arthroplasty. CASE REPORT A 69-year-old man with cerebral palsy presented with a 2-week history of gait difficulty and 3-day history of fever. At 12 and 9 months before presentation, he underwent left and right total knee arthroplasty, respectively. Both knees showed swelling, pain, and redness. The leukocyte count was 12 820/μL, and C-reactive protein level was 16.27 mg/dL. Bilateral aspiration revealed gram-positive cocci, confirming PJI, without implant loosening. We performed simultaneous debridement, irrigation, insert exchange, and CLAP on both knees. Intraoperative joint fluid cultures showed MRSA. Gentamicin was given intra-articularly for 14 days alongside intravenous vancomycin, which was continued for 31 days before switching to oral minocycline for 6 months. One year after surgery, the white blood cell count was 3400/µL, the C-reactive protein level was 0.08 mg/dL, and the range of motion was 0 to 130 degrees, with no recurrence of PJI. CONCLUSIONS CLAP represents a viable treatment option for PJI caused by MRSA after total knee arthroplasty.
    Oct. 2025, The American journal of case reports, 26, e949779, English, International magazine
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • ケースシリーズ 重症ピロン骨折に対する一期的足関節固定
    仲村 龍斗, 隈部 洋平, 澤内 健一, 福井 友章, 大江 啓介, 黒田 良祐
    (一社)中部日本整形外科災害外科学会, Oct. 2025, 中部日本整形外科災害外科学会雑誌, 68(秋季学会) (秋季学会), 202 - 202, Japanese

  • Jun Takagaki, Yohei Kumabe, Tomoaki Fukui, Ryosuke Kuroda, Keisuke Oe
    BACKGROUND The treatment of nonunion with deformity and shortening remains a significant challenge in orthopedic surgery. The chipping and lengthening technique is used for bone reconstruction and new bone formation, without the need for bone grafting. However, inadequate bone regeneration can require additional treatment. The Masquelet technique, a 2-stage procedure involving an induced membrane, has been reported to be effective in managing large bone defects and nonunion cases. CASE REPORT We present a case of a 21-year-old man with tibial nonunion associated with deformity and shortening. The patient underwent the chipping and lengthening technique using an external fixator, followed by conversion to internal fixation. However, bone regeneration at the distraction site was insufficient, necessitating salvage surgery using the Masquelet technique. The first-stage procedure involved debridement of the immature regenerate and placement of a cement spacer to induce membrane formation. In the second stage, the cement spacer was removed and replaced with an autologous bone graft. As a result, successful bone union was achieved, enabling the patient to regain full weight-bearing ability without complications. CONCLUSIONS This case highlights the effectiveness of the Masquelet technique as a salvage procedure for inadequate bone regeneration following the chipping and lengthening technique. The combination of these techniques is a viable treatment approach for nonunion cases with deformity and shortening.
    Sep. 2025, The American journal of case reports, 26, e948871, English, International magazine
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • 山本 裕也, 福井 友章, 大江 啓介, 隈部 洋平, 澤内 健一, 吉川 遼, 高瀬 恭平, 西田 亮太, 新倉 隆宏, 圓尾 明弘, 黒田 良祐
    (公社)日本整形外科学会, Sep. 2025, 日本整形外科学会雑誌, 99(8) (8), S1656 - S1656, Japanese

  • 近藤 飛馬, 福井 友章, 大江 啓介, 隈部 洋平, 福本 弦太, 西田 亮太, 山本 裕也, 黒田 良祐
    (公社)日本整形外科学会, Sep. 2025, 日本整形外科学会雑誌, 99(8) (8), S1759 - S1759, Japanese

  • 福本 弦太, 福井 友章, 大江 啓介, 隈部 洋平, Jonathan Jonathan, 近藤 飛馬, 西田 亮太, 山本 裕也, 圓尾 明弘, 黒田 良祐
    (公社)日本整形外科学会, Sep. 2025, 日本整形外科学会雑誌, 99(8) (8), S1773 - S1773, Japanese

  • 西田 亮太, 大江 啓介, 福井 友章, 隈部 洋平, 福本 弦太, Jonathan Jonathan, 近藤 飛馬, 山本 裕也, 新倉 隆宏, 黒田 良祐
    (公社)日本整形外科学会, Sep. 2025, 日本整形外科学会雑誌, 99(8) (8), S1787 - S1787, Japanese

  • 隈部 洋平, 西田 亮太, 松宮 豊, 福井 友章, 黒田 良祐, 大江 啓介
    (公社)日本整形外科学会, Sep. 2025, 日本整形外科学会雑誌, 99(8) (8), S1850 - S1850, Japanese

  • 福井 友章, 大江 啓介, 山本 裕也, 高瀬 恭平, 澤内 健一, 隈部 洋平, 黒田 良祐
    (公社)日本整形外科学会, Sep. 2025, 日本整形外科学会雑誌, 99(8) (8), S1931 - S1931, Japanese

  • ラット大腿骨iMAPモデルを用いた高濃度抗菌薬の影響の検討
    山本 裕也, 福井 友章, 大江 啓介, 隈部 洋平, 澤内 健一, 吉川 遼, 高瀬 恭平, 新倉 隆宏, 圓尾 明弘, 黒田 良祐
    (一社)日本骨・関節感染症学会, Jul. 2025, 日本骨・関節感染症学会プログラム・抄録集, 48回, 62 - 62, Japanese

  • ウサギを用いた222nm紫外線による術野殺菌効果の検討
    福井 友章, 大江 啓介, 隈部 洋平, 山本 裕也, 高瀬 恭平, 黒田 良祐
    (一社)日本骨・関節感染症学会, Jul. 2025, 日本骨・関節感染症学会プログラム・抄録集, 48回, 65 - 65, Japanese

  • 当院で治療した骨折関連感染症(FRI)の治療成績を用いたFRI分類の臨床的意義と成績不良因子の検討
    西田 亮太, 大江 啓介, 松宮 豊, 隈部 洋平, 福井 友章, 黒田 良祐
    (一社)日本骨・関節感染症学会, Jul. 2025, 日本骨・関節感染症学会プログラム・抄録集, 48回, 74 - 74, Japanese

  • PJI-TNM分類を用いた人工関節感染症の治療成績の検討
    隈部 洋平, 松宮 豊, 福井 友章, 大江 啓介, 黒田 良祐
    (一社)日本骨・関節感染症学会, Jul. 2025, 日本骨・関節感染症学会プログラム・抄録集, 48回, 87 - 87, Japanese

  • 当院におけるPJIの治療成績の検討 CLAP vs non-CLAP
    大江 啓介, 福井 友章, 隈部 洋平, 松宮 豊, 黒田 良祐
    (一社)日本骨・関節感染症学会, Jul. 2025, 日本骨・関節感染症学会プログラム・抄録集, 48回, 113 - 113, Japanese

  • Ryota Nishida, Tomoaki Fukui, Takahiro Niikura, Yohei Kumabe, Yutaka Matsumiya, Yuya Yamamoto, Hyuma Kondo, Jonathan Jonathan, Genta Fukumoto, Ryosuke Kuroda, Keisuke Oe
    BACKGROUND Lower-limb fracture malunion can result in angular deformity that requires surgical correction. Chipping corrective osteotomy (CCO) is a novel method that involves a chipping technique at the center of rotation of angulation, followed by single-stage internal fixation. This case series describes 3 cases of correction of fracture malunion of the lower limb managed using combined CCO and intramedullary nail stabilization. CASE REPORT Case 1: A 47-year-old man with a 17° varus deformity of the left tibia from a past accident had left knee pain. CCO was performed, achieving bone union in 4 months. The Mikulicz line improved from -11% to 23%, and his knee became pain-free. Case 2: A 59-year-old man with a 19° valgus deformity of the right tibia from a past accident had right knee pain. CCO was performed, achieving bone union in 11 months. The Mikulicz line improved from 119% to 72%, and he could perform daily activities pain-free. Case 3: A 59-year-old man with a complex left distal femoral deformity (25° varus and 15° internal rotation) from a past accident had left knee pain. CCO was performed, achieving bone union in 8 months. The Mikulicz line improved from -52% to 4%, and he started enjoying jogging. CONCLUSIONS We performed CCO combined with intramedullary nail stabilization in 3 cases of post-traumatic angular and rotational malunion, achieving good clinical outcomes. This single-stage surgery is advantageous for bone healing and is cost-effective, making it a viable option for correcting long-bone malunion.
    Jun. 2025, The American journal of case reports, 26, e947845, English, International magazine
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Kenichi Sawauchi, Keisuke Oe, Tomoaki Fukui, Yohei Kumabe, Hirotsugu Muratsu, Ryosuke Kuroda, Akihiro Maruo
    BACKGROUND Brodie's abscess is a rare form of subacute osteomyelitis that primarily affects the metaphyseal regions of long bones. Conventional treatment involves thorough curettage and systemic antibiotic therapy for ≥6 weeks, with bone grafting required for abscess sizes >3 cm. Continuous local antibiotic perfusion (CLAP) delivers high concentrations of antibiotics directly to the infection site and facilitates preservation of biologically active bone tissue. Here, we report a case of an extensive Brodie's abscess that was successfully treated with CLAP, highlighting its potential as an alternative to conventional treatment. CASE REPORT A 14-year-old boy was diagnosed with a 15-cm Brodie's abscess in the medial cortical bone of the left femur, with a primary infection in the intracortical pseudocavity. The patient underwent treatment with CLAP and intramedullary antibiotic perfusion pins were placed in the pseudocavity. Given the preservation of bone bioactivity, we anticipated spontaneous bone remodeling after infection control and therefore decided against bone grafting. The infection site received direct gentamicin infusions for 18 days, and systemic antibiotic therapy continued for 1 month. Postoperatively, the signs of inflammation rapidly subsided, and thereafter, no recurrence of infection was observed, while bone remodeling was achieved at the site of the bone defect. CONCLUSIONS In cases of cortical bone abscess, systemically administered antibiotics have limited penetration, whereas CLAP allows direct administration of high-concentration antibiotics to the lesion, which may provide an advantage in infection control. The findings of this case suggest that CLAP may serve as a viable treatment option for Brodie's abscess, particularly in cases with limited systemic antibiotic penetration.
    Mar. 2025, The American journal of case reports, 26, e947099, English, International magazine
    Scientific journal

  • Ryota Nishida, Tomoaki Fukui, Kenichi Sawauchi, Yohei Kumabe, Hyuma Kondo, Yuya Yamamoto, Kyohei Takase, Ryo Yoshikawa, Takahiro Niikura, Ryosuke Kuroda, Keisuke Oe
    Transcutaneous CO2 application promotes fracture healing and osteogenesis via angiogenesis. However, its molecular mechanism remains unclear. This therapy transiently decreases intra-tissue pH in the affected area to approximately 7.0 for 20 min, followed by recovery. We hypothesized that such intermittent pH changes activate endothelial cells similarly to acidic preconditioning. We aimed to investigate the response of human umbilical vein endothelial cells (HUVECs) to daily intermittent low-pH stimulation. HUVECs were cultured under three conditions: daily 20-min exposure to a low-pH medium (approximately 7.0), followed by a return to a control medium (approximately 7.4) (Change group); continuous exposure to a low-pH medium (Low pH group); constant culturing in a control medium (Control group). Cell proliferation, tube formation, migration, and protein/gene expression were assessed. Tube formation and migration were significantly enhanced in the Change group compared with those in the Control group, and tube formation was also increased in the Low pH group. Western blotting revealed upregulated expression of vascular endothelial growth factor (VEGF) and VEGF receptor 2 in the Change group. CD31 gene expression was elevated in the Low pH and Change groups on Days 1 and 7, respectively. Phosphorylated extracellular signal-regulated kinases 1 and 2 (ERK1/2) and protein kinase B (AKT) levels in the Low pH and Change groups were significantly increased compared with those in the Control, except for ERK2 in the Low pH group. The suppression of ERK1/2 or AKT by their inhibitors (U0126 and LY294002, respectively) during low-pH exposure inhibited proliferation and tube formation, indicating that the ERK1/2 mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3 kinase (PI3K)/AKT pathways mediate endothelial cell activation in response to intermittent acidic stimulation. Intermittent low-pH exposure enhanced HUVEC activity, mediated by the ERK1/2 MAPK and PI3K/AKT pathways. Our findings provide insights into the mechanism by which transcutaneous CO2 application promotes angiogenesis and tissue repair.
    2025, PloS one, 20(9) (9), e0332673, English, International magazine
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Ryota Nishida, Tomoaki Fukui, Takahiro Niikura, Yohei Kumabe, Ryo Yoshikawa, Kyohei Takase, Yuya Yamamoto, Ryosuke Kuroda, Keisuke Oe
    We previously demonstrated that transcutaneous CO2 application promotes muscle fiber-type switching, fracture healing, and osteogenesis by increasing blood flow and angiogenesis. Here, we aimed to investigate the preventive effects of transcutaneous CO2 application on disuse osteoporosis and muscle atrophy in a rat hindlimb suspension model. Eleven-week-old male Sprague-Dawley rats were divided into hindlimb suspension (HS), HS with transcutaneous CO2 application (HSCO2), and control groups. HSCO2 rats were administered transcutaneous 100 % CO2 gas in their bilateral hindlimbs, five times a week for 20 min. After 3 weeks, we harvested the gastrocnemius, femur, and tibia for assessment. Histological analysis revealed a significant decrease in the gastrocnemius myofiber cross-sectional area in HS rats compared to the control rats, whereas HSCO2 rats exhibited a significant increase compared to HS rats. Micro-computed tomography showed significant bone atrophy in the trabecular and cortical bones of the femur in HS rats compared to those of the control rats, whereas significant improvement was noted in HSCO2 rats. Histological analysis of the proximal tibia revealed more marrow adipose tissue in the HS rats than in the control rats. However, in the HSCO2 rats, fewer marrow adipose tissue and osteoclasts were observed. Moreover, HSCO2 rats had more osteoblasts and higher expression of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) and vascular endothelial growth factor (VEGF) than the HS rats. The gastrocnemius and distal femur of HSCO2 rats also exhibited elevated PGC-1α and VEGF expression and upregulation of the myogenesis markers and osteogenesis markers compared to those of HS rats. This treatment effectively prevented disuse osteoporosis and muscle atrophy by promoting local angiogenesis and blood flow. PGC-1α is crucial for promoting this angiogenic pathway. Transcutaneous CO2 application may be a novel preventive procedure for disuse osteoporosis and muscle atrophy, complementing medication and rehabilitation.
    Dec. 2024, Bone, 189, 117262 - 117262, English, International magazine
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Kyohei Takase, Takahiro Niikura, Tomoaki Fukui, Yohei Kumabe, Kenichi Sawauchi, Ryo Yoshikawa, Yuya Yamamoto, Ryota Nishida, Tomoyuki Matsumoto, Ryosuke Kuroda, Keisuke Oe
    The interconnected structures in a 3D scaffold allows the movement of cells and nutrients. Therefore, this study aimed to investigate the in-vivo bioactivity of 3D-printed β-tricalcium phosphate (β-TCP) and hydroxyapatite (HAP) scaffolds that replicate biological bone. This study included 24-week-old male New Zealand white rabbits. A cylindrical bone defect with a diameter of 4.5 mm and a depth of 8 mm was created in the lateral aspect of the distal femur. A 3D-printed scaffold was implanted in the right femur (experimental side), whereas the left femur was kept free of implantation (control side). Micro-CT analysis and histological observations of the bone defect site were conducted at 4, 8, and 12 weeks postoperatively to track the bone repair progress. No evidence of new bone tissue formation was found in the medullary cavity of the bone defect on the control side. In contrast, on the experimental side, the 3D scaffold demonstrated sufficient bioactivity, leading to the growth of new bone tissue. Over time, new bone tissue gradually extended from the periphery toward the center, a phenomenon evident in both micro-CT images and biopsy staining. In the current study, we observed that the cells involved in bone metabolism adhered, spread, and proliferated on our newly designed 3D-printed scaffold with a bone microstructure. Therefore, it is suggested that this scaffold has sufficient bioactivity to induce new bone formation and could be expected to be a more useful artificial bone than the existing version.
    Sep. 2024, Journal of materials science. Materials in medicine, 35(1) (1), 53 - 53, English, International magazine
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Yannik Kalbas, Yohei Kumabe, Felix Karl-Ludwig, Sascha Halvachizadeh, Michel Paul Johan Teuben, Miriam Weisskopf, Nikola Cesarovic, Andreas J Hülsmeier, Sonja Märsmann, Christian Hierholzer, Frank Hildebrand, Thorsten Hornemann, Roman Pfeifer, Paolo Cinelli, Hans-Christoph Pape
    INTRODUCTION: Along with recent advances in analytical technologies, tricarboxylic acid-cycle intermediates are increasingly identified as promising makers for cellular ischemia and mitochondrial dysfunction during hemorrhagic shock. For traumatized patients, the knowledge of the role of lipid oxidation substrates is sparse. In this study, we aimed to analyze the dynamics of systemic acylcarnitine (AcCa) release in a standardized polytrauma model with hemorrhagic shock. METHODS: Fifty-two male pigs (50 ± 5 kg) were randomized into two groups: group isolated fracture was subject to a standardized femur shaft fracture, and group polytrauma was subject to a femur fracture, followed by blunt chest trauma, liver laceration, and a pressure-controlled hemorrhagic shock for 60 minutes. Resuscitation was performed with crystalloids. Fractures were stabilized by intramedullary nailing. Venous samples were collected at six time points (baseline, trauma, resuscitation, 2 hours, 4 hours, and 6 hours). Lipidomic analysis was performed via liquid chromatography coupled mass spectrometry. Measurements were collated with clinical markers and near-infrared spectrometry measurements of tissue perfusion. Longitudinal analyses were performed with linear mixed models, and Spearman's correlations were calculated. A p value of 0.05 was defined as threshold for statistical significance. RESULTS: From a total of 303 distinct lipids, we identified two species of long-chain AcCas. Both showed a highly significant ( p < 0.001) twofold increase after hemorrhagic shock in group polytrauma that promptly normalized after resuscitation. This increase was associated with a significant decrease of the base excess ( p = 0.005), but recovery after resuscitation was faster. For both AcCas, there were significant correlations with decreased muscle tissue oxygen delivery ( p = 0.008, p = 0.003) and significant time-lagged correlations with the increase of creatine kinase ( p < 0.001, p < 0.001). CONCLUSION: Our results point to plasma AcCas as a possible indicator for mitochondrial dysfunction and cellular ischemia in hemorrhagic shock. The more rapid normalization after resuscitation in comparison with acid base changes may warrant further investigation.
    Aug. 2024, The journal of trauma and acute care surgery, 97(2) (2), 248 - 257, English, International magazine
    Scientific journal

  • 大江 啓介, 福井 友章, 隈部 洋平, 松宮 豊, 新倉 隆宏, 黒田 良祐
    (一社)日本整形外傷学会, Jun. 2024, 骨折, 46(Suppl.) (Suppl.), S71 - S71, Japanese

  • 山本 裕也, 福井 友章, 大江 啓介, 隈部 洋平, 澤内 健一, 吉川 遼, 高瀬 恭平, 西田 亮太, 近藤 飛馬, 新倉 隆宏, 黒田 良祐
    (一社)日本整形外傷学会, Jun. 2024, 骨折, 46(Suppl.) (Suppl.), S142 - S142, Japanese

  • 福井 友章, 大江 啓介, 隈部 洋平, 松宮 豊, 新倉 隆宏, 黒田 良祐
    (一社)日本整形外傷学会, Jun. 2024, 骨折, 46(Suppl.) (Suppl.), S245 - S245, Japanese

  • 西田 亮太, 大江 啓介, 福井 友章, 隈部 洋平, 近藤 飛馬, 山本 裕也, 高瀬 恭平, 新倉 隆宏, 黒田 良祐
    (一社)日本整形外傷学会, Jun. 2024, 骨折, 46(Suppl.) (Suppl.), S258 - S258, Japanese

  • 近藤 飛馬, 福井 友章, 大江 啓介, 西田 亮太, 山本 裕也, 高瀬 恭平, 隈部 洋平, 新倉 隆弘, 黒田 良祐
    (一社)日本整形外傷学会, Jun. 2024, 骨折, 46(Suppl.) (Suppl.), S311 - S311, Japanese

  • 隈部 洋平, 大江 啓介, 福井 友章, 松宮 豊, 黒田 良祐
    (一社)日本整形外傷学会, Jun. 2024, 骨折, 46(Suppl.) (Suppl.), S377 - S377, Japanese

  • Yannik Kalbas, Felix Karl-Ludwig Klingebiel, Sascha Halvachizadeh, Yohei Kumabe, Julian Scherer, Michel Teuben, Roman Pfeifer, Hans-Christoph Pape
    PURPOSE: Although the term "major fracture" is commonly used in the management of trauma patients, it is defined insufficiently to date. The polytrauma section of ESTES is trying to develop a more standardized use and a definition of the term. In this process, a standardized literature search was undertaken. We test the hypothesis that the understanding of "major fractures" has changed and is modified by a better understanding of patient physiology. METHODS: A systematic literature search of the Medline and EMBASE databases was conducted in March 2022. Original studies that investigated surgical treatment strategies in polytraumatized patients with fractures were included: This included timing, sequence and type of operative treatment. A qualitative synthesis regarding the prevalence of anatomic regions of interest and core factors determining decision-making was performed. Data were stratified by decades. RESULTS: 4278 articles were identified. Of these, 74 were included for qualitative evaluation: 50 articles focused on one anatomic region, 24 investigated the relevance of multiple anatomic regions. Femur fractures were investigated most frequently (62) followed by pelvic (22), spinal (15) and tibial (15) fractures. Only femur (40), pelvic (5) and spinal (5) fractures were investigated in articles with one anatomic region of interest. Before 2010, most articles focused on long bone injuries. After 2010, fractures of pelvis and spine were cited more frequently. Additional determining factors for decision-making were covered in 67 studies. These included chest injuries (42), TBI (26), hemorrhagic shock (25) and other injury-specific factors (23). Articles before 2000 almost exclusively focused on chest injury and TBI, while shock and injury-specific factors (e.g., soft tissues, spinal cord injury, and abdominal trauma) became more relevant after 2000. CONCLUSION: Over time, the way "major fractures" influenced surgical treatment strategies has changed notably. While femur fractures have long been the only focus, fixation of pelvic and spinal fractures have become more important over the last decade. In addition to the fracture location, associated conditions and injuries (chest trauma and head injuries) influence surgical decision-making as well. Hemodynamic stability and injury-specific factors (soft tissue injuries) have increased in importance over time.
    Jun. 2024, European journal of trauma and emergency surgery : official publication of the European Trauma Society, 50(3) (3), 657 - 669, English, International magazine
    Scientific journal

  • Yuya Yamamoto, Tomoaki Fukui, Kenichi Sawauchi, Ryo Yoshikawa, Kyohei Takase, Yohei Kumabe, Akihiro Maruo, Takahiro Niikura, Ryosuke Kuroda, Keisuke Oe
    AIMS: Continuous local antibiotic perfusion (CLAP) has recently attracted attention as a new drug delivery system for orthopaedic infections. CLAP is a direct continuous infusion of high-concentration gentamicin (1,200 μg/ml) into the bone marrow. As it is a new system, its influence on the bone marrow is unknown. This study aimed to examine the effects of high-concentration antibiotics on human bone tissue-derived cells. METHODS: Cells were isolated from the bone tissue grafts collected from six patients using the Reamer-Irrigator-Aspirator system, and exposed to different gentamicin concentrations. Live cells rate, apoptosis rate, alkaline phosphatase (ALP) activity, expression of osteoblast-related genes, mineralization potential, and restoration of cell viability and ALP activity were examined by in vitro studies. RESULTS: The live cells rate (the ratio of total number of cells in the well plate to the absorbance-measured number of live cells) was significantly decreased at ≥ 500 μg/ml of gentamicin on day 14; apoptosis rate was significantly increased at ≥ 750 μg/ml, and ALP activity was significantly decreased at ≥ 750 μg/ml. Real-time reverse transcription-polymerase chain reaction results showed no significant decrease in the ALP and activating transcription factor 4 transcript levels at ≥ 1,000 μg/ml on day 7. Mineralization potential was significantly decreased at all concentrations. Restoration of cell viability was significantly decreased at 750 and 1,000 μg/ml on day 21 and at 500 μg/ml on day 28, and ALP activity was significantly decreased at 500 μg/ml on day 28. CONCLUSION: Our findings suggest that the exposure concentration and duration of antibiotic administration during CLAP could affect cell functions. However, further in vivo studies are needed to determine the optimal dose in a clinical setting.
    Mar. 2024, Bone & joint research, 13(3) (3), 91 - 100, English, International magazine
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Yohei Kumabe, Yannik Kalbas, Sascha Halvachizadeh, Michel Teuben, Nikola Cesarovic, Miriam Weisskopf, Andreas Hülsmeier, Thorsten Hornemann, Paolo Cinelli, Hans-Christoph Pape, Roman Pfeifer
    BACKGROUND: Occult hypoperfusion describes the absence of sufficient microcirculation despite normal vital signs. It is known to be associated with prolonged elevation of serum lactate and later complications in severely injured patients. We hypothesized that changes in circulating lipids are related to responsiveness to resuscitation. The purpose of this study is investigating the relation between responsiveness to resuscitation and lipidomic course after poly trauma. METHODS: Twenty-five male pigs were exposed a combined injury of blunt chest trauma, liver laceration, controlled haemorrhagic shock, and femoral shaft fracture. After 1 h, animals received resuscitation and fracture stabilization. Venous blood was taken regularly and 233 specific lipids were analysed. Animals were divided into two groups based on serum lactate level at the end point as an indicator of responsiveness to resuscitation (<2 mmol/L: responder group (R group), ≧2 mmol/L: occult hypoperfusion group (OH group)). RESULTS: Eighteen animals met criteria for the R group, four animals for the OH group, and three animals died. Acylcarnitines showed a significant increase at 1 h compared to baseline in both groups. Six lipid subgroups showed a significant increase only in R group at 2 h. There was no significant change at other time points. CONCLUSIONS: Six lipid groups increased significantly only in the R group at 2 h, which may support the idea that they could serve as potential biomarkers to help us to detect the presence of occult hypoperfusion and insufficient resuscitation. We feel that further study is required to confirm the role and mechanism of lipid changes after trauma.
    Feb. 2024, European journal of trauma and emergency surgery : official publication of the European Trauma Society, 50(1) (1), 107 - 114, English, International magazine
    Scientific journal

  • Kosuke Sugiyama, Kiyotaka Kurachi, Masaki Sano, Kyota Tatsuta, Tadahiro Kojima, Toshiya Akai, Katsunori Suzuki, Kakeru Torii, Mayu Sakata, Yoshifumi Morita, Hirotoshi Kikuchi, Yoshihiro Hiramatsu, Yohei Kumabe, Keisuke Oe, Tomoaki Fukui, Rena Kaigome, Masahiro Sasaki, Toru Koi, Hiroyuki Ohashi, Tetsuro Suzuki, Ryosuke Kuroda, Hiroya Takeuchi
    Far ultraviolet-C irradiation at 222 nm has potent bactericidal effects against severe infections such as peritonitis, with minimal cytotoxicity. Bacterial peritonitis due to bowel perforation is a serious condition with high mortality despite current treatments. This study investigated the safety and efficacy of intraperitoneal far ultraviolet-C irradiation at 222 nm. In vitro experiments optimized the fluid conditions for bacterial or protein concentrations prior to in vivo evaluation. In vivo efficacy was assessed in a rat peritonitis model induced by Escherichia coli, measuring intra-abdominal bacterial concentration, blood cytokine levels, and mortality rates. Safety was evaluated by analyzing cyclobutane pyrimidine dimers as markers of DNA damage in five abdominal organs: stomach, small intestine, colon, liver, and spleen. Statistical analyses employed parametric methods for normally distributed data and non-parametric methods for data without normality. Optimal in vitro conditions included 106 CFU/mL bacteria, 0.5 mW/cm2 irradiation, and 10-3 mg/mL protein. In the rat model, far ultraviolet-C irradiation at 222 nm significantly decreased intra-abdominal bacteria, reduced blood cytokines (interleukin-1β and interleukin-6), and elevated survival rates from 20% to 60%, compared to lavage alone. The formation of cyclobutane pyrimidine dimers was significantly lower with 222 nm irradiation than with 254 nm, suggesting reduced DNA damage. These findings indicate that far ultraviolet-C irradiation at 222 nm, when combined with lavage, represents a promising therapeutic strategy for bacterial peritonitis, providing effective bacterial reduction and a favorable safety profile. Further research is needed to verify these findings and investigate long-term safety and potential clinical applications.
    2024, PloS one, 19(11) (11), e0311552, English, International magazine
    Scientific journal

  • Felix Karl-Ludwig Klingebiel, Morgan Hasegawa, Oliver Strähle, Yannik Kalbas, Michel Teuben, Sascha Halvachizadeh, Yohei Kumabe, Hans-Christoph Pape, Roman Pfeifer
    PURPOSE: It is known that the magnitude of surgery and timing of surgical procedures represents a crucial step of care in polytraumatized patients. In contrast, it is not clear which specific factors are most critical when evaluating the surgical load (physiologic burden to the patient incurred by surgical procedures). Additionally, there is a dearth of evidence for which body region and surgical procedures are associated with high surgical burden. The aim of this study was to identify key factors and quantify the surgical load for different types of fracture fixation in multiple anatomic regions. METHODS: A standardized questionnaire was developed by experts from Société Internationale de Chirurgie Orthopédique et de Traumatologie (SICOT)-Trauma committee. Questions included relevance and composition of the surgical load, operational staging criteria, and stratification of operation procedures in different anatomic regions. Quantitative values according to a five-point Likert scale were chosen by the correspondents to determine the surgical load value based on their expertise. The surgical load for different surgical procedures in different body regions could be chosen in a range between "1," defined as the surgical load equivalent to external (monolateral) fixator application, and "5," defined as the maximal surgical load possible in that specific anatomic region. RESULTS: This questionnaire was completed online by 196 trauma surgeons from 61 countries in between Jun 26, 2022, and July 16, 2022 that are members of SICOT. The surgical load (SL) overall was considered very important by 77.0% of correspondents and important by 20.9% correspondents. Intraoperative blood loss (43.2%) and soft tissue damage (29.6%) were chosen as the most significant factors by participating surgeons. The decision for staged procedures was dictated by involved body region (56.1%), followed by bleeding risk (18.9%) and fracture complexity (9.2%). Percutaneous or intramedullary procedures as well as fractures in distal anatomic regions, such as hands, ankles, and feet, were consistently ranked lower in their surgical load. CONCLUSION: This study demonstrates a consensus in the trauma community about the crucial relevance of the surgical load in polytrauma care. The surgical load is ranked higher with increased intraoperative bleeding and greater soft tissue damage/extent of surgical approach and depends relevantly on the anatomic region and kind of operative procedure. The experts especially consider anatomic regions and the risk of intraoperative bleeding as well as fracture complexity to guide staging protocols. Specialized guidance and teaching is required to assess both the patient's physiological status and the estimated surgical load reliably in the preoperative decision-making and operative staging.
    Jul. 2023, International orthopaedics, 47(7) (7), 1677 - 1687, English, International magazine
    Scientific journal

  • Yannik Kalbas, Sascha Halvachizadeh, Yohei Kumabe, Anna Theresa Luidl, Jennifer Lynne Steel, Boris A Zelle, Paolo Cinelli, Hans-Christoph Pape, Roman Pfeifer
    PURPOSE: There is limited research on the long-term psychiatric outcomes of polytraumatized patients. Existing studies focus mainly on the negative sequelae. Post-traumatic growth (PTG) describes positive personal development after severe physical or mental distress. In this study, we investigated post-traumatic growth in polytraumatized patients at least 20 years after trauma. METHODS: Patients treated for polytrauma at a German level 1 trauma center between 1971 and 1990, were contacted 20+ years later. A questionnaire with 37 questions from the stress-related growth scale (SRGS) and the post-traumatic growth inventory (PGI) was administered. PTG was quantified in five specific areas. PTG and patient demographics were then analyzed using logistic regression. RESULTS: Eligible questionnaires were returned by 337 patients. 96.5% of patients reported improvements regarding at least one of the 37 questions. Approximately, a third of patients noticed distinct improvements regarding their relationship to others (29.2%), appreciation of life (36.2%) and attitudes towards new possibilities (32.5%). Patient demographics were significant predictors for the development of PTG: Older (p < 0.001), female (p = 0.042) and married patients (p = 0.047) showed a greater expression of PTG. We also saw significantly more PTG in patients with higher injury severity (p = 0.033). CONCLUSION: 20 years after polytrauma, patients report improvements in their relationship with others, appreciation of life and attitude towards new possibilities. Women and married patients show higher expression of PTG. Furthermore, there is higher expression of PTG with higher age and injury severity. Post-traumatic growth should be identified and fostered in clinical practice. LEVEL OF EVIDENCE: III-prospective long-term follow-up study.
    Jun. 2023, European journal of trauma and emergency surgery : official publication of the European Trauma Society, 49(3) (3), 1279 - 1286, English, International magazine
    Scientific journal

  • Ryo Yoshikawa, Tomoaki Fukui, Keisuke Oe, Yohei Kumabe, Takahiro Oda, Kenichi Sawauchi, Kyohei Takase, Yuya Yamamoto, Yoshitada Sakai, Ryosuke Kuroda, Takahiro Niikura
    In this study, we examined the proliferation capability and osteogenic and chondrogenic differentiation potential of non-hypertrophic nonunion cells (NHNCs), and the effect of Escherichia coli-derived BMP-2 (E-BMP-2) on them. We enrolled five patients with non-hypertrophic nonunion. NHNCs isolated from nonunion tissue sampled during surgery were cultured, passaged, counted every 14 days, and analyzed. NHNCs were homogenous fibroblastic adherent cells and long-lived through at least 10 passages, with a slight decline. The cells were consistently positive for mesenchymal stem cell-related markers CD73 and CD105, and negative for the hematopoietic markers CD14 and CD45. NHNCs could differentiate into osteoblast lineage cells; however, they did not have strong calcification or sufficient chondrogenic differentiation capability. E-BMP-2 did not affect the proliferative capability of the cells but improved their osteogenic differentiation capability by increasing alkaline phosphatase activity and upregulating the gene expression of osterix, bone sialoprotein, and osteocalcin. E-BMP-2 enhanced their chondrogenic differentiation capability by upregulating the gene expression of aggrecan and collagen type II. We showed, for the first time, that NHNCs have the capacity to differentiate into osteoblast-lineage cells, although the chondrogenic differentiation potential was poor. Local application of E-BMP-2 with preservation of nonunion tissue is a potential treatment option for non-hypertrophic nonunion.
    Nov. 2022, Current issues in molecular biology, 44(11) (11), 5562 - 5578, English, International magazine
    Scientific journal

  • Tomoaki Fukui, Takahiro Niikura, Takahiro Oda, Yohei Kumabe, Aiko Nishiaki, Rena Kaigome, Hiroyuki Ohashi, Masahiro Sasaki, Tatsushi Igarashi, Keisuke Oe, Michael R Hamblin, Ryosuke Kuroda
    For the prevention of surgical site infection (SSI), continuous disinfection could be helpful. Short wavelength ultraviolet radiation C (UVC) is highly bactericidal but shows cytotoxicity. Radiation of UVC with a wavelength of 222 nm to the skin is considered to be safe because it only reaches the stratum corneum. However, the safety of 222 nm irradiation to the surgical field not covered with skin is unknown. The purpose of this study was to examine the safety of 222 nm UVC irradiation on a surgical field in a rabbit model. Five types of tissue were surgically exposed and irradiated with 222 or 254 nm UVC. Immunohistological assessment against cyclobutane pyrimidine dimer (CPD), an index of DNA damage by UVC, was performed. The CPD-positive cell rate was significantly higher in the 254 nm group than in the other groups in all tissues. A 222 nm group showed significantly more CPD than control in fat tissue, but no significant difference in all other tissues. In fat tissue collected 24 h after irradiation, the 254 nm group showed higher CPD than the other groups, while the 222 nm group had reduced to the control level. These data suggest that 222 nm UVC irradiation could be a new method to safely prevent SSI.
    Nov. 2022, Photochemistry and photobiology, 98(6) (6), 1365 - 1371, English, International magazine
    Scientific journal

  • Yannik Kalbas, Thomas Seaver, Yohei Kumabe, Sascha Halvachizadeh, Maximilian Lempert, Roman Pfeifer, Andrew Marcantonio, Hans-Christoph Pape
    OBJECTIVES: Fat embolism and fat embolism syndrome (FES) remain common complications following long bone fractures. Incidence is highest after bilateral femur fractures. We performed a systematic review of FES after bilateral femur fractures and present two cases. DATA SOURCES: Systematic literature search of the Cochrane, EMBASE, MEDLINE, Scopus, and, Web of Science Library databases was performed in August 2021. Terms used including plural and alternate spellings: "fat embolism,""fat embolism syndrome,""fat embolus," and "bilateral femur fracture." Articles in German and English were considered. No time frame was applied. STUDY SELECTION: Original studies, case series and case reports on fat embolism after bilateral femur fracture were included. Insufficient documentation or patients with relevant previous heath conditions were excluded. DATA EXTRACTION: Abstracts were organized using EndNote X9 by Carivate. Three authors independently screened the abstracts; cross check of the extracted data was performed by the senior author. DATA SYNTHESIS: Scarcity of articles only allowed for a qualitative synthesis. Data was compared with our cases and situated within the scientific background. RESULTS: Ten articles were included for qualitative synthesis (n = 144 patients). The symptoms were inhomogeneous with neurological deficits being most prominent. Degree of displacement was high, when reported. Although the modes and timing of surgery varied, this appeared unrelated with outcome. CONCLUSIONS: FES remains a relevant complication after bilateral femur fractures, despite damage control strategies and improved reaming techniques. Fracture displacement and reduction maneuvers might play a more substantial role in the formation than previously accredited. LEVEL OF EVIDENCE: 4.
    Apr. 2022, OTA international : the open access journal of orthopaedic trauma, 5(2 Suppl) (2 Suppl), e187, English, International magazine
    Scientific journal

  • Kenichi Sawauchi, Tomoaki Fukui, Keisuke Oe, Yohei Kumabe, Takahiro Oda, Ryo Yoshikawa, Kyohei Takase, Takehiko Matsushita, Tomoyuki Matsumoto, Shinya Hayashi, Ryosuke Kuroda, Takahiro Niikura
    Recently, reamer-irrigator-aspirator (RIA) systems have been increasingly used to harvest autologous bone grafts. RIA graft materials contain bone marrow, which provides a viable source to derive large numbers of mesenchymal stem cells. Low-intensity pulsed ultrasound (LIPUS) significantly accelerates the differentiation of stem cells derived from bone marrow. This in vitro study investigated the effect of LIPUS on the osteogenic activity and differentiation of RIA graft-derived cells. A small amount of RIA graft was obtained from seven patients. After the cells derived from RIA grafts were cultured, they were divided into two groups: the LIPUS and control groups. LIPUS was applied once daily for 20 min (1.5 MHz, pulse duration: 200 µs, pulse repetition rate: 1 kHz, spatial average-temporal average intensity: 30 mW/cm2). Alkaline phosphatase activity (113.4% and 130.1% on days 7 and 14), expression of osteoblast-related genes (ALP, Runx2) and mineralization (135.2% on day 21) of the RIA graft-derived cells were significantly higher in the LIPUS group than in the control group. However, LIPUS did not affect the cell proliferation of RIA graft-derived cells. This study indicates that LIPUS may enhance the healing of non-union and critical bone defects treated by autologous bone grafting using the RIA system.
    Feb. 2022, Ultrasound in medicine & biology, 48(2) (2), 313 - 322, English, International magazine
    Scientific journal

  • Yohei Kumabe, Keisuke Oe, Masakazu Morimoto, Naomi Yagi, Tomoaki Fukui, Ryosuke Kuroda, Yutaka Hata, Takahiro Niikura
    Correct assessment of the bone healing process is required for the management of limb immobilization during the treatment of bone injuries, including fractures and defects. Although the monitoring of bone healing using ultrasound poses several advantages regarding cost and ionizing radiation exposure compared with other dominant imaging methods, such as radiography and computed tomography (CT), traditional ultrasound B-mode imaging lacks reliability and objectivity. However, the body structures can be quantitatively observed by ultrasound frequency-based methods, and therefore, the disadvantages of B-mode imaging can be overcome. In this study, we created a femoral bone hole model of a rat and observed the bone healing process using the quantitative ultrasound method and micro-CT, which provides a reliable assessment of the tissue microstructure of the bone. This study analyzed the correlation between these two assessments. The results revealed that the quantitative ultrasound measurements correlated with the CT measurements for rat bone healing. This ultrasound frequency-based method could have the potential to serve as a novel modality for quantitative monitoring of bone healing with the advantages of being less invasive and easily accessible. Impact statement Bone healing monitoring with ultrasound is advantageous as it is less invasive and easily accessible; however, the traditional B-mode method lacks reliability and objectivity. This study demonstrated that the proposed ultrasound frequency-based monitoring method can quantitatively observe bone healing and strongly correlates with the computed tomography measurements for rat bone healing. This method has the potential to become a reliable modality for monitoring bone healing.
    Jun. 2021, Tissue engineering. Part C, Methods, 27(6) (6), 349 - 356, English, International magazine
    Scientific journal

  • Takahiro Oda, Takahiro Niikura, Tomoaki Fukui, Keisuke Oe, Yu Kuroiwa, Yohei Kumabe, Kenichi Sawauchi, Ryo Yoshikawa, Yutaka Mifune, Shinya Hayashi, Tomoyuki Matsumoto, Takehiko Matsushita, Teruya Kawamoto, Yoshitada Sakai, Toshihiro Akisue, Ryosuke Kuroda
    INTRODUCTION: Diabetes mellitus (DM) negatively affects fracture repair by inhibiting endochondral ossification, chondrogenesis, callus formation, and angiogenesis. We previously reported that transcutaneous CO2 application accelerates fracture repair by promoting endochondral ossification and angiogenesis. The present study aimed to determine whether CO2 treatment would promote fracture repair in cases with type I DM. RESEARCH DESIGN AND METHODS: A closed femoral shaft fracture was induced in female rats with streptozotocin-induced type I DM. CO2 treatment was performed five times a week for the CO2 group. Sham treatment, where CO2 was replaced with air, was performed for the control group. Radiographic, histologic, genetic, and biomechanical measurements were taken at several time points. RESULTS: Radiographic assessment demonstrated that fracture repair was induced in the CO2 group. Histologically, accelerated endochondral ossification and capillary formation were observed in the CO2 group. Immunohistochemical assessment indicated that early postfracture proliferation of chondrocytes in callus was enhanced in the CO2 group. Genetic assessment results suggested that cartilage and bone formation, angiogenesis, and vasodilation were upregulated in the CO2 group. Biomechanical assessment revealed enhanced mechanical strength in the CO2 group. CONCLUSIONS: Our findings suggest that CO2 treatment accelerates fracture repair in type I DM rats. CO2 treatment could be an effective strategy for delayed fracture repair due to DM.
    Dec. 2020, BMJ open diabetes research & care, 8(2) (2), English, International magazine
    Scientific journal

  • Yohei Kumabe, Tomoaki Fukui, Shunsuke Takahara, Yu Kuroiwa, Michio Arakura, Keisuke Oe, Takahiro Oda, Kenichi Sawauchi, Takehiko Matsushita, Tomoyuki Matsumoto, Shinya Hayashi, Ryosuke Kuroda, Takahiro Niikura
    BACKGROUND: Distraction osteogenesis has been broadly used to treat various structural bone deformities and defects. However, prolonged healing time remains a major problem. Various approaches including the use of low-intensity pulsed ultrasound, parathyroid hormone, and bone morphogenetic proteins (BMPs) have been studied to shorten the treatment period with limited success. Our previous studies of rats have reported that the transcutaneous application of CO2 accelerates fracture repair and bone-defect healing in rats by promoting angiogenesis, blood flow, and endochondral ossification. This therapy may also accelerate bone generation during distraction osteogenesis, but, to our knowledge, no study investigating CO2 therapy on distraction osteogenesis has been reported. QUESTIONS/PURPOSES: We aimed to investigate the effect of transcutaneous CO2 during distraction osteogenesis in rabbits, which are the most suitable animal as a distraction osteogenesis model for a lengthener in terms of limb size. We asked: Does transcutaneous CO2 during distraction osteogenesis alter (1) radiographic bone density in the distraction gap during healing; (2) callus parameters, including callus bone mineral content, volumetric bone mineral density, and bone volume fraction; (3) the newly formed bone area, cartilage area, and angiogenesis, as well as the expression of interleukin-6 (IL-6), BMP-2, BMP-7, hypoxia-inducible factor (HIF) -1α, and vascular endothelial growth factor (VEGF); and (4) three-point bend biomechanical strength, stiffness, and energy? METHODS: Forty 24-week-old female New Zealand white rabbits were used according to a research protocol approved by our institutional ethical committee. A distraction osteogenesis rabbit tibia model was created as previously described. Briefly, an external lengthener was applied to the right tibia, and a transverse osteotomy was performed at the mid-shaft. The osteotomy stumps were connected by adjusting the fixator to make no gap. After a 7-day latency phase, distraction was continued at 1 mm per day for 10 days. Beginning the day after the osteotomy, a 20-minute transcutaneous application of CO2 on the operated leg using a CO2 absorption-enhancing hydrogel was performed five times per week in the CO2 group (n = 20). Sham treatment with air was administered in the control group (n = 20). Animals were euthanized immediately after the distraction period (n = 10), 2 weeks (n = 10), and 4 weeks (n = 20) after completion of distraction. We performed bone density quantification on the plain radiographs to evaluate consolidation in the distraction gap with image analyzing software. Callus parameters were measured with micro-CT to assess callus microstructure. The newly formed bone area and cartilage area were measured histologically with safranin O/fast green staining to assess the progress of ossification. We also performed immunohistochemical staining of endothelial cells with fluorescein-labeled isolectin B4 and examined capillary density to evaluate angiogenesis. Gene expressions in newly generated callus were analyzed by real-time polymerase chain reaction. Biomechanical strength, stiffness, and energy were determined from a three-point bend test to assess the mechanical strength of the callus. RESULTS: Radiographs showed higher pixel values in the distracted area in the CO2 group than the control group at Week 4 of the consolidation phase (0.98 ± 0.11 [95% confidence interval 0.89 to 1.06] versus 1.19 ± 0.23 [95% CI 1.05 to 1.34]; p = 0.013). Micro-CT demonstrated that bone volume fraction in the CO2 group was higher than that in the control group at Week 4 (5.56 ± 3.21 % [95% CI 4.32 to 6.12 %] versus 11.90 ± 3.33 % [95% CI 9.63 to 14.25 %]; p = 0.035). There were no differences in any other parameters (that is, callus bone mineral content at Weeks 2 and 4; volumetric bone mineral density at Weeks 2 and 4; bone volume fraction at Week 2). At Week 2, rabbits in the CO2 group had a larger cartilage area compared with those in the control group (2.09 ± 1.34 mm [95% CI 1.26 to 2.92 mm] versus 5.10 ± 3.91 mm [95% CI 2.68 to 7.52 mm]; p = 0.011). More newly formed bone was observed in the CO2 group than the control group at Week 4 (68.31 ± 16.32 mm [95% CI 58.19 to 78.44 mm] versus 96.26 ± 19.37 mm [95% CI 84.25 to 108.26 mm]; p < 0.001). There were no differences in any other parameters (cartilage area at Weeks 0 and 4; newly formed bone area at Weeks 0 and 2). Immunohistochemical isolectin B4 staining showed greater capillary densities in rabbits in the CO2 group than the control group in the distraction area at Week 0 and surrounding tissue at Weeks 0 and 2 (distraction area at Week 0, 286.54 ± 61.55 /mm [95% CI 232.58 to 340.49] versus 410.24 ± 55.29 /mm [95% CI 361.78 to 458.71]; p < 0.001; surrounding tissue at Week 0 395.09 ± 68.16/mm [95% CI 335.34 to 454.83] versus 589.75 ± 174.42/mm [95% CI 436.86 to 742.64]; p = 0.003; at Week 2 271.22 ± 169.42 /mm [95% CI 122.71 to 419.73] versus 508.46 ± 49.06/mm [95% CI 465.45 to 551.47]; p < 0.001 respectively). There was no difference in the distraction area at Week 2. The expressions of BMP -2 at Week 2, HIF1-α at Week 2 and VEGF at Week 0 and 2 were greater in the CO2 group than in the control group (BMP -2 at Week 2 3.84 ± 0.83 fold [95% CI 3.11 to 4.58] versus 7.32 ± 1.63 fold [95% CI 5.88 to 8.75]; p < 0.001; HIF1-α at Week 2, 10.49 ± 2.93 fold [95% CI 7.91 to 13.06] versus 20.74 ± 11.01 fold [95% CI 11.09 to 30.40]; p < 0.001; VEGF at Week 0 4.80 ± 1.56 fold [95% CI 3.43 to 6.18] versus 11.36 ± 4.82 fold [95% CI 7.13 to 15.59]; p < 0.001; at Week 2 31.52 ± 8.26 fold [95% CI 24.27 to 38.76] versus 51.05 ± 15.52 fold [95% CI 37.44 to 64.66]; p = 0.034, respectively). There were no differences in any other parameters (BMP-2 at Week 0 and 4; BMP -7 at Weeks 0, 2 and 4; HIF-1α at Weeks 0 and 4; IL-6 at Weeks 0, 2 and 4; VEGF at Week 4). In the biomechanical assessment, ultimate stress and failure energy were greater in the CO2 group than in the control group at Week 4 (ultimate stress 259.96 ± 74.33 N [95% CI 167.66 to 352.25] versus 422.45 ± 99.32 N [95% CI 299.13 to 545.77]; p < 0.001, failure energy 311.32 ± 99.01 Nmm [95% CI 188.37 to 434.25] versus 954.97 ± 484.39 Nmm [95% CI 353.51 to 1556.42]; p = 0.003, respectively). There was no difference in stiffness (216.77 ± 143.39 N/mm [95% CI 38.73 to 394.81] versus 223.68 ± 122.17 N/mm [95% CI 71.99 to 375.37]; p = 0.92). CONCLUSION: Transcutaneous application of CO2 accelerated bone generation in a distraction osteogenesis model of rabbit tibias. As demonstrated in previous studies, CO2 treatment might affect bone regeneration in distraction osteogenesis by promoting angiogenesis, blood flow, and endochondral ossification. CLINICAL RELEVANCE: The use of the transcutaneous application of CO2 may open new possibilities for shortening healing time in patients with distraction osteogenesis. However, a deeper insight into the mechanism of CO2 in the local tissue is required before it can be used in future clinical practice.
    Aug. 2020, Clinical orthopaedics and related research, 478(8) (8), 1922 - 1935, English, International magazine
    Scientific journal

  • Tomoaki Fukui, Takahiro Niikura, Takahiro Oda, Yohei Kumabe, Hiroyuki Ohashi, Masahiro Sasaki, Tatsushi Igarashi, Makoto Kunisada, Nozomi Yamano, Keisuke Oe, Tomoyuki Matsumoto, Takehiko Matsushita, Shinya Hayashi, Chikako Nishigori, Ryosuke Kuroda
    INTRODUCTION: Surgical site infection is one of the most severe complications of surgical treatments. However, the optimal procedure to prevent such infections remains uninvestigated. Ultraviolet radiation C (UVC) with a short wavelength has a high bactericidal effect; however, it is cytotoxic. Nonetheless, given that UVC with a wavelength of 222 nm reaches only the stratum corneum, it does not affect the skin cells. This study aimed to investigate the safety of 222-nm UVC irradiation and to examine its skin sterilization effect in healthy volunteers. METHODS: This trial was conducted on 20 healthy volunteers. The back of the subject was irradiated with 222-nm UVC at 50-500 mJ/cm2, and the induced erythema (redness of skin) was evaluated. Subsequently, the back was irradiated with a maximum amount of UVC not causing erythema, and the skin swabs before and after the irradiation were cultured. The number of colonies formed after 24 hours was measured. In addition, cyclobutene pyrimidine dimer (CPD) as an indicator of DNA damage was measured using skin tissues of the nonirradiated and irradiated regions. RESULTS: All subjects experienced no erythema at all doses. The back of the subject was irradiated at 500 mJ/cm2, and the number of bacterial colonies in the skin swab culture was significantly decreased by 222-nm UVC irradiation. The CPD amount produced in the irradiated region was slightly but significantly higher than that of the non-irradiated region. CONCLUSION: A 222-nm UVC at 500 mJ/cm2 was a safe irradiation dose and possessed bactericidal effects. In the future, 222-nm UVC irradiation is expected to contribute to the prevention of perioperative infection.
    2020, PloS one, 15(8) (8), e0235948, English, International magazine
    Scientific journal

  • Yu Kuroiwa, Tomoaki Fukui, Shunsuke Takahara, Sang Yang Lee, Keisuke Oe, Michio Arakura, Yohei Kumabe, Takahiro Oda, Tomoyuki Matsumoto, Takehiko Matsushita, Toshihiro Akisue, Yoshitada Sakai, Ryosuke Kuroda, Takahiro Niikura
    BACKGROUND: Bone defects may occur because of severe trauma, nonunion, infection, or tumor resection. However, treatments for bone defects are often difficult and have not been fully established yet. We previously designed an efficient system of topical cutaneous application of carbon dioxide (CO2) using a novel hydrogel, which facilitates CO2 absorption through the skin into the deep area within a limb. In this study, the effect of topical cutaneous application of CO2 on bone healing was investigated using a rat femoral defect model. METHODS: In this basic research study, an in vivo bone defect model, fixed with an external fixator, was created using a rat femur. The affected limb was shaved, and CO2 was applied for 20 min/day, 5 days/week. In the control animals, CO2 gas was replaced with air. Radiographic, histological, biomechanical, and genetic assessments were performed to evaluate bone healing. RESULTS: Radiographically, bone healing rate was significantly higher in the CO2 group than in the control group at 4 weeks (18.2% vs. 72.7%). The degree of bone healing scored using the histopathological Allen grading system was significantly higher in the CO2 group than in the control group at 2 weeks (1.389 ± 0.334 vs. 1.944 ± 0.375). The ultimate stress, extrinsic stiffness, and failure energy were significantly greater in the CO2 group than in the control group at 4 weeks (3.2 ± 0.8% vs. 38.1 ± 4.8%, 0.6 ± 0.3% vs. 41.5 ± 12.2%, 2.6 ± 0.8% vs. 24.7 ± 5.9%, respectively.). The volumetric bone mineral density of the callus in micro-computed tomography analysis was significantly higher in the CO2 group than in the control group at 4 weeks (180.9 ± 43.0 mg/cm3 vs. 247.9 ± 49.9 mg/cm3). Gene expression of vascular endothelial growth factor in the CO2 group was significantly greater than that in the control group at 3 weeks (0.617 ± 0.240 vs. 2.213 ± 0.387). CONCLUSIONS: Topical cutaneous application of CO2 accelerated bone healing in a rat femoral defect model. CO2 application can be a novel and useful therapy for accelerating bone healing in bone defects; further research on its efficacy in humans is warranted.
    May 2019, BMC musculoskeletal disorders, 20(1) (1), 237 - 237, English, International magazine
    Scientific journal

  • Yohei Kumabe, Sang Yang Lee, Takahiro Waki, Takashi Iwakura, Shunsuke Takahara, Michio Arakura, Yu Kuroiwa, Tomoaki Fukui, Tomoyuki Matsumoto, Takehiko Matsushita, Kotaro Nishida, Ryosuke Kuroda, Takahiro Niikura
    BACKGROUND: Some reports have shown that intermittent parathyroid hormone (PTH) (1-34) treatment for patients with delayed union or nonunion have led to successful healing. In this study, we investigated whether systemic intermittent administration of PTH (1-34) has a beneficial effect on bone healing in a rat refractory fracture model. METHODS: We created a refractory femoral fracture model in 32 rats with periosteal cauterization that leads to atrophic nonunion at 8 weeks after surgery. Half the rats received subcutaneous intermittent human PTH (1-34) injections at a dosage of 100 μg/kg, thrice a week for 8 weeks. The other half received the vehicle only. At 8 weeks after fracture, radiographic, histological and mechanical assessments were performed. RESULTS: Radiographic assessments showed that the union rate was significantly higher in the PTH group than in the control group (P < 0.05). The degree of fracture repair as scored using the Allen grading system in histological assessment was significantly greater in the PTH group than in the control group (P < 0.05). The ultimate stress and stiffness measurements were significantly greater in the PTH group than in the control group (p < 0.05). CONCLUSIONS: We demonstrated that triweekly administration of PTH (1-34) increased union rate and accelerated bone healing in a rat refractory fracture model, suggesting that systemic administration of PTH (1-34) could become a novel and useful therapy for accelerating fracture healing in patients at high risk of delayed union or nonunion.
    Dec. 2017, BMC musculoskeletal disorders, 18(1) (1), 545 - 545, English, International magazine
    Scientific journal

■ MISC
  • CLAPにおけるゲンタマイシン濃度による細胞傷害と腎機能障害のリスク因子ついて
    大江啓介, 福井友章, 隈部洋平, 山本裕也, 新倉隆宏, 黒田良祐
    2025, 中部日本整形外科災害外科学会雑誌, 68

  • 大きな回旋変形を伴う小児大腿骨骨幹部骨折後偽関節の1例
    吉田圭助, 隈部洋平, 大江啓介, 福井友章
    2025, 中部日本整形外科災害外科学会雑誌, 68

  • 同側の変形性股関節症を合併した非定型大腿骨骨折の治療経過についての検討
    福井友章, 大江啓介, 隈部洋平, 松宮豊, 新倉隆宏, 黒田良祐
    2025, 日本整形外科学会雑誌(CD-ROM), 99(3) (3)

  • 当院における人工関節周囲感染に対する持続局所抗菌薬灌流療法の治療成績の検討
    大江啓介, 福井友章, 隈部洋平, 松宮豊, 松本知之, 林申也, 黒田良祐
    2025, 日本整形外科学会雑誌(CD-ROM), 99(3) (3)

  • 有限要素法を用いたRIA(reamer irrigator aspirator)が大腿骨力学強度に与える影響の解析
    隈部洋平, 西田亮太, 松宮豊, 福井友章, 大江啓介, 黒田良祐
    2025, 日本整形外科学会雑誌(CD-ROM), 99(3) (3)

  • 当院における人工関節周囲感染に対する持続局所抗菌薬灌流療法の治療成績
    大江啓介, 福井友章, 隈部洋平, 松宮豊, 松本知之, 林申也, 美舩泰, 黒田雄一, 壺坂正徳, 亀長智幸, 黒田良祐
    2025, 日本人工関節学会プログラム・抄録集, 55th (CD-ROM)

  • 大江啓介, 新倉隆宏, 福井友章, 隈部洋平, 松本知之, 黒田良祐
    金原出版, 30 Apr. 2024, 整形・災害外科, 67(5) (5), 681 - 687

  • 細菌性腹膜炎への腹腔内222nmUVC照射による腹水中細菌制御効果と安全性の検証
    杉山洸裕, 立田協太, 小嶋忠浩, 赤井俊也, 鈴木克徳, 鳥居翔, 阪田麻裕, 森田剛文, 菊池寛利, 平松良浩, 隈部洋平, 大江啓介, 福井友章, 黒田良祐, 倉地清隆, 竹内裕也
    2024, 日本外科学会定期学術集会(Web), 124th

  • 非定型大腿骨骨折において骨転移に対する骨修飾薬使用歴の有無が治療経過に与える影響
    福井友章, 大江啓介, 隈部洋平, 新倉隆宏, 黒田良祐
    2024, 日本整形外科学会雑誌, 98(2) (2)

  • CLAPにおけるゲンタマイシンの薬物動態に関する検討
    松宮豊, 大江啓介, 福井友章, 隈部洋平, 黒田良祐
    2024, 日本整形外科学会雑誌, 98(2) (2)

  • ブタ多発外傷モデルにおける循環血流のリピドミクス解析
    隈部洋平, 隈部洋平, KALBAS Yannik, HALVACHIZADEH Sascha, HORNEMANN Thorsten, PFEIFER Roman, CINELLI Paolo, PAPE Hans-Christoph
    2024, 日本整形外科学会雑誌, 98(3) (3)

  • 膝関節が露出した膝蓋骨骨折術後感染をCLAPと腓腹筋弁によりサルベージした一例
    藤原悠, 大江啓介, 福井友章, 隈部洋平, 黒田良祐
    2024, 中部日本整形外科災害外科学会雑誌, 67

  • 骨折治療後長期に排膿を繰り返す慢性骨髄炎に対する持続局所抗菌薬灌流療法の短期治療成績
    大江啓介, 福井友章, 隈部洋平, 松宮豊, 黒田良祐
    2024, 日本骨・関節感染症学会プログラム・抄録集, 47th

  • Investigation of a new bone regeneration therapy using the Masquelet technique for rabbit femoral bone defects
    山本裕也, 福井友章, 大江啓介, 隈部洋平, 澤内健一, 吉川遼, 高瀬恭平, 西田亮太, 近藤飛馬, 新倉隆宏, 黒田良祐
    2024, 骨折(Web), 46(Supplement (CD-ROM)) (Supplement (CD-ROM))

  • Can RIA products be an alternative to autologous iliac cancellous bone grafts?
    大江啓介, 福井友章, 隈部洋平, 松宮豊, 新倉隆宏, 黒田良祐
    2024, 骨折(Web), 46(Supplement (CD-ROM)) (Supplement (CD-ROM))

  • 骨折関連感染症治療における持続局所抗菌薬潅流(CLAP)のゲンタマイシンの投与濃度に関する検討-多施設研究-
    大江啓介, 福井友章, 隈部洋平, 松宮豊, 山本裕也, 高原俊介, 善家雄吉, 姫野大輔, 山下伸之輔, 松本匡洋, 山川泰明, 森井北斗, 新倉隆宏, 圓尾明弘
    2024, 日本骨・関節感染症学会プログラム・抄録集, 47th

  • 人工足関節置換術後感染に対し持続局所抗菌薬灌流療法を併用した足関節固定術が奏功した1例
    松宮豊, 大江啓介, 福井友章, 隈部洋平, 黒田良祐
    2024, 日本骨・関節感染症学会プログラム・抄録集, 47th

  • Three cases of chipping corrective osteotomies with intramedullary nail for reconstruction of malunion
    西田亮太, 大江啓介, 福井友章, 隈部洋平, 近藤飛馬, 山本裕也, 高瀬恭平, 新倉隆宏, 黒田良祐
    2024, 骨折(Web), 46(Supplement (CD-ROM)) (Supplement (CD-ROM))

  • Treatment of atypical femoral fractures combined with ipsilateral hip osteoarthritis
    福井友章, 大江啓介, 隈部洋平, 松宮豊, 新倉隆宏, 黒田良祐
    2024, 骨折(Web), 46(Supplement (CD-ROM)) (Supplement (CD-ROM))

  • 2 cases of internal infection using mesh plate for proximal phalanx pseudarthrosis of the great toe
    近藤飛馬, 福井友章, 大江啓介, 西田亮太, 山本裕也, 高瀬恭平, 隈部洋平, 新倉隆弘, 黒田良祐
    2024, 骨折(Web), 46(Supplement (CD-ROM)) (Supplement (CD-ROM))

  • Nonunion with rotational deformity after pediatric femoral shaft fracture
    隈部洋平, 大江啓介, 福井友章, 松宮豊, 黒田良祐
    2024, 骨折(Web), 46(Supplement (CD-ROM)) (Supplement (CD-ROM))

  • 間欠的な細胞外低pH環境がヒト臍帯静脈内皮細胞活性に及ぼす影響
    西田亮太, 福井友章, 大江啓介, 隈部洋平, 近藤飛馬, 山本裕也, 高瀬恭平, 吉川遼, 新倉隆宏, 黒田良祐
    2024, 日本整形外科学会雑誌(CD-ROM), 98(8) (8)

  • 廃用性骨粗鬆症ラットの骨折モデルにおける炭酸ガス経皮吸収療法の効果の検討
    近藤飛馬, 福井友章, 大江啓介, 隈部洋平, 西田亮太, 山本裕也, 高瀬恭平, 黒田良祐
    2024, 日本整形外科学会雑誌(CD-ROM), 98(8) (8)

  • 新たなウサギMasquelet法モデルを用いた自家骨移植とBMP-2投与の骨再生効果の比較
    山本裕也, 福井友章, 大江啓介, 隈部洋平, 澤内健一, 吉川遼, 高瀬恭平, 西田亮太, 近藤飛馬, 新倉隆宏, 黒田良祐
    2024, 日本整形外科学会雑誌(CD-ROM), 98(8) (8)

  • 骨折治癒における炭酸ガス経皮吸収療法の至適時間の検討
    近藤飛馬, 高瀬恭平, 福井友章, 大江啓介, 隈部洋平, 西田亮太, 山本裕也, 吉川遼, 新倉隆宏, 黒田良祐
    2024, 日本整形外科学会雑誌(CD-ROM), 98(8) (8)

  • 後肢懸垂ラットによる廃用性骨萎縮・筋萎縮に対する炭酸ガス経皮吸収の予防効果
    西田亮太, 福井友章, 大江啓介, 隈部洋平, 近藤飛馬, 山本裕也, 高瀬恭平, 吉川遼, 新倉隆宏, 黒田良祐, 黒田良祐
    2024, 日本整形外科学会雑誌(CD-ROM), 98(8) (8)

  • 当院における人工関節感染に対する持続局所抗菌薬灌流療法の治療成績
    大江啓介, 福井友章, 隈部洋平, 松本知之, 林申也, 黒田良祐
    2024, 中部日本整形外科災害外科学会雑誌, 67

  • RIA(Reamer Irrigator Aspirator)が大腿骨の力学強度に与える影響に関する有限要素解析
    隈部洋平, 松宮豊, 福井友章, 大江啓介, 黒田良祐
    2024, 中部日本整形外科災害外科学会雑誌, 67

  • 人工足関節置換術後の破綻に対し金属スペーサーを用いて関節固定を行った2例
    松宮豊, 大江啓介, 福井友章, 隈部洋平, 黒田良祐
    2024, 中部日本整形外科災害外科学会雑誌, 67

  • 外傷後変形癒合に対してchipping corrective osteotomyを施行した3例
    西田亮太, 大江啓介, 福井友章, 隈部洋平, 新倉隆宏, 黒田良祐
    2024, 中部日本整形外科災害外科学会雑誌, 67

  • 脆弱性骨折モデルにおける炭酸ガス経皮吸収療法の効果の検討
    近藤飛馬, 福井友章, 大江啓介, 隈部洋平, 山本裕也, 黒田良祐
    2024, 中部日本整形外科災害外科学会雑誌, 67

  • インプラント周囲非定型大腿骨骨折の病態と治療戦略
    福井友章, 大江啓介, 隈部洋平, 松宮豊, 新倉隆宏, 黒田良祐
    2024, 中部日本整形外科災害外科学会雑誌, 67

  • ウサギ大腿骨Masquelet法モデルを用いた自家骨とBMP-2の骨再生能の比較
    山本裕也, 福井友章, 大江啓介, 隈部洋平, 新倉隆宏, 黒田良祐
    2024, 中部日本整形外科災害外科学会雑誌, 67

  • 短縮・変形を伴った脛骨偽関節に対して,Chipping法及びHexapod型創外固定を用いて緩徐矯正を行った一例
    高垣潤, 隈部洋平, 松宮豊, 福井友章, 大江啓介, 黒田良佑
    2024, 中部日本整形外科災害外科学会雑誌, 67

  • Non-hypertrophic nonunionには骨分化能を有する細胞が存在し,E-BMP-2により骨・軟骨分化能が促進される
    吉川遼, 新倉隆宏, 福井友章, 大江啓介, 隈部洋平, 小田崇弘, 澤内健一, 高瀬恭平, 山本裕也, 酒井良忠, 黒田良祐
    2022, 日本整形外科学会雑誌, 96(8) (8)

  • The effect of transcutaneous CO2 application on distraction osteogenesis of rabbit tibia
    隈部洋平, 新倉隆宏, 大江啓介, 福井友章, 黒田良祐
    2020, 日本創外固定・骨延長学会雑誌, 31

  • 健常人における222nmUVC照射の安全性と殺菌効果の検討
    福井友章, 新倉隆宏, 小田崇弘, 隈部洋平, 大江啓介, 黒田良祐
    2020, 日本整形外科学会雑誌, 94(2) (2)

  • ウサギを用いた222nm紫外線術野照射の安全性の検討
    福井友章, 新倉隆宏, 小田崇弘, 隈部洋平, 大江啓介, 黒田良祐
    2020, 日本整形外科学会雑誌, 94(8) (8)

  • Reamer irrigator aspirator(RIA)産物から得た細胞の骨分化に対するlow-intensity pulsed ultrasound(LIPUS)の効果
    澤内健一, 新倉隆宏, 福井友章, 大江啓介, 吉川遼, 小田崇弘, 隈部洋平, 黒田良祐
    2020, 日本整形外科学会雑誌, 94(8) (8)

  • 骨折治癒過程を超音波の周波数特性により推定する試み
    大江啓介, 隈部洋平, 新倉隆宏, 福井友章, 黒田良祐, 畑豊, 森本雅和, 八木直美, 小矢美晴
    2020, 日本整形外科学会雑誌, 94(8) (8)

  • 卵巣摘出ラット骨粗鬆症モデルにおける,炭酸ガス経皮吸収による骨粗鬆症改善効果の検討
    澤内健一, 新倉隆宏, 福井友章, 大江啓介, 吉川遼, 小田崇弘, 隈部洋平, 黒田良祐
    2020, 日本整形外科学会雑誌, 94(8) (8)

  • 炭酸ガス経皮吸収は1型糖尿病ラット骨折モデルにおける骨折治癒を促進させる
    小田崇弘, 新倉隆宏, 福井友章, 大江啓介, 隈部洋平, 澤内健一, 吉川遼, 酒井良忠, 黒田良祐
    2020, 日本整形外科学会雑誌, 94(8) (8)

  • 男性骨粗鬆症モデルラットに対する炭酸ガス経皮吸収の影響に関する検討
    吉川遼, 新倉隆宏, 福井友章, 大江啓介, 澤内健一, 小田崇弘, 隈部洋平, 酒井良忠, 黒田良祐
    2020, 日本整形外科学会雑誌, 94(8) (8)

  • 骨延長術における炭酸ガス経皮吸収の骨形成促進効果について
    隈部洋平
    2019, Japanese Journal of Rehabilitation Medicine, 56(Supplement) (Supplement)

  • 炭酸ガス経皮吸収は1型糖尿病ラット骨折モデルにおいて骨癒合を促進させる
    小田崇弘, 新倉隆宏, 福井友章, 大江啓介, 新倉路生, 黒岩祐, 隈部洋平, 澤内健一, 酒井良忠, 黒田良祐
    2019, 日本整形外科学会雑誌, 93(8) (8)

  • 炭酸ガス経皮吸収はウサギ脛骨骨延長術における骨形成を促進する
    隈部洋平, 新倉隆宏, 福井友章, 高原俊介, 黒岩祐, 新倉路生, 大江啓介, 小田崇弘, 澤内健一, 黒田良祐
    2019, 日本整形外科学会雑誌, 93(8) (8)

  • ウサギ骨延長術モデルにおける炭酸ガス経皮吸収の骨形成促進効果について
    隈部洋平, 新倉隆宏, 大江啓介, 福井友章, 黒田良祐
    2019, 移植(Web), 54(4-5) (4-5)

  • 222nm紫外線照射の安全性および殺菌効果
    福井友章, 新倉隆宏, 小田崇弘, 隈部洋平, 大江啓介, 黒田良祐
    2018, 日本骨・関節感染症学会プログラム・抄録集, 41st

  • 炭酸ガス経皮吸収がラットの筋組織に及ぼす影響
    大江啓介, 新倉隆宏, 福井友章, 黒岩祐, 隈部洋平, 小田崇弘, 澤内健一, 酒井良忠, 黒田良祐
    2018, 日本整形外科学会雑誌, 92(8) (8)

  • 222nm紫外線照射の安全性と殺菌効果の検討
    福井友章, 新倉隆宏, 小田崇弘, 隈部洋平, 大江啓介, 黒田良祐
    2018, 日本整形外科学会雑誌, 92(8) (8)

  • ヒトiPS細胞による軟骨内骨化を介した骨再生療法
    新倉路生, 李相亮, 李相亮, 福井友章, 大江啓介, 高原俊介, 黒岩祐, 隈部洋平, 黒田良祐, 新倉隆宏
    2018, 日本整形外科学会雑誌, 92(8) (8)

  • ラット骨欠損モデルにおけるCO2経皮吸収の骨形成促進作用についての検討
    黒岩祐, 新倉隆宏, 高原俊介, 高原俊介, 李相亮, 李相亮, 大江啓介, 福井友章, 新倉路生, 隈部洋平, 小田崇弘, 酒井良忠, 黒田良祐
    2018, 日本整形外科学会雑誌, 92(8) (8)

  • 骨延長術における炭酸ガス経皮吸収の骨形成促進効果について
    隈部洋平, 新倉隆宏, 福井友章, 大江啓介, 高原俊介, 新倉路生, 黒岩祐, 小田崇弘, 黒田良祐
    2018, 日本再生医療学会総会(Web), 17th

  • 骨折治癒過程におけるmicroRNAの発現:糖尿病・健常ラツト間の比較検討
    高原俊介, 李相亮, 新倉隆宏, 大江啓介, 福井友章, 新倉路生, 黒岩祐, 隈部洋平, 酒井良忠, 黒田良祐
    2017, 日本整形外科学会雑誌, 91(8) (8)

  • CO2経皮吸収はラット骨欠損モデルにおける骨形成を促進させる
    黒岩祐, 新倉隆宏, 高原俊介, 李相亮, 大江啓介, 岩倉崇, 福井友章, 新倉路生, 隈部洋平, 酒井良忠, 黒田良祐
    2017, 日本整形外科学会雑誌, 91(8) (8)

  • CO2経皮吸収はラット骨欠損モデルにおいて骨形成を促進させる
    小田崇弘, 黒岩祐, 高原俊介, 新倉路生, 隈部洋平, 李相亮, 大江啓介, 岩倉崇, 福井友章, 酒井良忠, 新倉隆宏, 黒田良祐
    2017, 移植(Web), 52(6) (6)

  • 肘部管症候群手術症例における術後1年での電気生理学的回復について
    隈部洋平, 金谷貴子, 名倉一成, 美舩泰, 国分毅, 鷲見正敏
    2016, 臨床整形外科, 51(1) (1)

  • 腰部脊柱管狭窄症に対する低侵襲後方椎体間固定術の術後成績と隣接椎間障害の検討
    隈部洋平, 青木謙二, 原田俊彦, 高山博行, 岸本健太, 西原寛玄
    2016, 中部日本整形外科災害外科学会雑誌, 59

  • KUMABE Yohei
    The Central Japan Association of Orthopaedic Surgery and Traumatology, 2016, The Central Japan Journal of Orthopaedic Surgery & Traumatology, 59(4) (4), 697 - 698, Japanese

  • 隈部 洋平, 金谷 貴子, 名倉 一成
    東京 : 医学書院, Jan. 2016, 臨床整形外科, 51(1) (1), 5 - 8, Japanese

  • 橈骨頭・頚部骨折の治療成績
    隈部洋平, 新倉路生, 伊藤研二郎, 松島真司, 吉田和也
    2013, 中部日本整形外科災害外科学会雑誌, 56

  • 当院における整形外科領域の感染症についての検討
    新倉路生, 吉田和也, 松島真司, 伊藤研二郎, 隈部洋平
    2013, 中部日本整形外科災害外科学会雑誌, 56

  • KUMABE Yohei
    The Central Japan Association of Orthopaedic Surgery and Traumatology, 2013, The Central Japan Journal of Orthopaedic Surgery & Traumatology, 56(3) (3), 613 - 614, Japanese

  • MATUSHIMA Shinji
    The Central Japan Association of Orthopaedic Surgery and Traumatology, 2013, The Central Japan Journal of Orthopaedic Surgery & Traumatology, 56(6) (6), 1375 - 1376, Japanese

  • KUMABE Yohei
    The Central Japan Association of Orthopaedic Surgery and Traumatology, 2013, The Central Japan Journal of Orthopaedic Surgery & Traumatology, 56(6) (6), 1369 - 1370, Japanese

■ Research Themes
  • Development of a therapeutic strategy for enhancing fracture healing through macrophage modulation
    隈部 洋平, 大江 啓介, 田畑 泰彦, 福井 友章, 澤内 健一
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 01 Apr. 2025 - 31 Mar. 2030

  • 炭酸ガスを用いた新規骨再生療法の開発
    福井 友章, 大江 啓介, 隈部 洋平, 澤内 健一
    日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2025 - 31 Mar. 2029

  • 骨関節感染症に対する持続局所抗菌薬灌流療法の安全性と有効性の検証
    大江 啓介, 隈部 洋平, 福井 友章, 澤内 健一
    日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2025 - 31 Mar. 2028

  • マクロファージの極性調整による骨折治癒促進療法の開発
    公益財団法人 整形災害外科学研究助成財団, 研究助成, Apr. 2025 - Mar. 2027, Principal investigator

  • シルクエラスチンを用いた骨折治癒促進療法の開発
    一般社団法人 日本損害保険協会, 交通事故医療研究助成, Sep. 2024 - Aug. 2025, Principal investigator

  • Mechanoluminescent Based Monitoring for Fracture Healing
    隈部 洋平
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Research Activity Start-up, Kobe University, 31 Aug. 2023 - 31 Mar. 2025
    応力発光技術を用いて動物モデルにおける骨折癒合過程を観察することを目的に、実験方法を定めるための予備実験を行なった。他実験で使用済みの動物の余剰骨を利用した。 当初ラットを用いて実験を行う計画であったが、実際に検証を行ったところ、サイズが小さく市販の動物骨折固定プレートとの適合性が悪かった。また試験機を用いて力学的評価を行うため、骨の両端をレジンで台座に固定する必要があるが、サイズが小さいため手技が困難であり、また固定部分が少ないため、荷重時に十分な安定性が得られなかった。そのため、ラットより大型であるウサギの大腿骨を実験対象として検証することとした。ウサギ大腿骨は市販の骨折固定プレートとの適合性が良好であった。また、両端のレジンによる台座への固定も容易であり、力学試験を遂行するのに十分な固定性を得ることができた。採取されたウサギ大腿骨にボーンソーを用いて骨幹部骨折を作成、応力発光塗料を塗布したプレートで骨折部を固定し、両端をレジンで固定したうえで力学試験機で軸圧をかけて発光を観察した。固定が破綻することなく、良好な応力発光を観察することができた。プレートサイズ（規格、スクリュー本数等）、力学試験機設定（荷重量、荷重速度等）、応力発光観察環境（測定器設置数や位置、結果解析方法等）などを調整し、実験に最適な条件を決定した。当初予定していたラットから変更し、ウサギを実験動物として使用することを決定した。

  • 自家組織を犠牲にしない新規骨再生療法の開発
    新倉 隆宏, 大江 啓介, 福井 友章, 澤内 健一, 隈部 洋平
    日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 01 Apr. 2022 - 31 Mar. 2025
    本研究の目的は、自家骨移植に取って代わる、将来臨床で実用可能な「自家組織を犠牲にしない新規骨再生療法の開発」である。令和5年度の研究実施計画は（１）BMPと人工骨、induced membraneを利用した骨再生に関するin vivo試験を継続することに加え、（２）3D構造再現・表面加工人工骨の開発と、それを用いた骨再生に関するin vitro試験、in vivo試験を行うことであった。（１）については、ウサギを用いた実験で大腿骨骨幹部に骨欠損を作成し実験を行った。骨セメントを欠損部に4週間留置することでinduced membraneを作成することができた。骨欠損部を架橋プレート固定し骨欠損部に自家腸骨を移植することで骨癒合する動物モデルを確立した。これをコントロールとし、自家骨移植の代わりにBMP-2含有人工骨（beta-tricalcium phosphate: beta-TCP）を充填した実験群とで骨再生をX線学的、組織学的に評価した。さらに摘出大腿骨の力学的強度を比較検討した。その結果、BMP-2含有人工骨の自家骨に対する非劣勢が認められた。自家骨移植にとって代わる、採骨という侵襲を患者に与えない新たな骨再生療法開発に繋がる有意義な結果を得ることができた。（２）については、ウサギの大腿骨遠位部の微細構造を3Dプリンタで再現する人工骨を作成することに成功した。in vitro試験で細胞毒性に問題なく、細胞接着が良好であることを確認した。hydroxyapatite, beta-TCPを基材として作成できている。これをウサギの大腿骨遠位部に作成した骨孔に埋植しX線学的、組織学的に評価したところ、いずれにおいても良好な骨再生を示した。両者を比較するとbeta-TCPの方がより良い骨再生が認められた。3D人工骨作成においてbeta-TCPが適切な基材となることが分かった。

  • Development of a new preventive and therapeutic method for locomotive syndrome using carbon dioxide
    Fukui Tomoaki
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 01 Apr. 2021 - 31 Mar. 2025
    This study investigated the effects of transcutaneous carbon dioxide therapy on disuse-induced osteoporosis and muscle atrophy using an animal model. In hindlimb suspension rats, the treatment improved bone density, bone strength, and muscle cross-sectional area, while increasing osteoblast activity and suppressing osteoclasts. Additionally, the influence of extracellular pH changes on angiogenesis and cell migration was reproduced in vitro, providing insight into the therapy’s mechanism of action. The therapy also improved fracture healing rates in a femoral fracture model, suggesting its potential as a non-invasive and effective treatment option.

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