Research activity information

• RalA, PLD and mTORC1 Are Required for Kinase-Independent Pathways in DGKβ-Induced Neurite Outgrowth.

  Takuya Kano, Ryosuke Tsumagari, Akio Nakashima, Ushio Kikkawa, Shuji Ueda, Minoru Yamanoue, Nobuyuki Takei, Yasuhito Shirai

  Diacylglycerol kinase β (DGKβ) is an enzyme that converts diacylglycerol to phosphatidic acid and is mainly expressed in the cerebral cortex, hippocampus and striatum. We previously reported that DGKβ induces neurite outgrowth and spinogenesis, contributing to higher brain functions, including emotion and memory. To elucidate the mechanisms involved in neuronal development by DGKβ, we investigated the importance of DGKβ activity in the induction of neurite outgrowth using human neuroblastoma SH-SY5Y cells. Interestingly, both wild-type DGKβ and the kinase-negative (KN) mutant partially induced neurite outgrowth, and these functions shared a common pathway via the activation of mammalian target of rapamycin complex 1 (mTORC1). In addition, we found that DGKβ interacted with the small GTPase RalA and that siRNA against RalA and phospholipase D (PLD) inhibitor treatments abolished DGKβKN-induced neurite outgrowth. These results indicate that binding of RalA and activation of PLD and mTORC1 are involved in DGKβKN-induced neurite outgrowth. Taken together with our previous reports, mTORC1 is a key molecule in both kinase-dependent and kinase-independent pathways of DGKβ-mediated neurite outgrowth, which is important for higher brain functions.

  Dec. 2021, Biomolecules, 11(12) (12), English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The S. pombe CDK5 Orthologue Pef1 Cooperates with Three Cyclins, Clg1, Pas1 and Psl1, to Promote Pre-Meiotic DNA Replication.

  Shinya Matsuda, Ushio Kikkawa, Akio Nakashima

  Meiosis is a specialized cell division process that mediates genetic information transfer to the next generation. Meiotic chromosomal segregation occurs when DNA replication is completed during the pre-meiotic S phase. Here, we show that Schizosaccharomyces pombe Pef1, an orthologue of mammalian cyclin-dependent kinase 5 (CDK5), is required to promote pre-meiotic DNA replication. We examined the efficiency of meiotic initiation using pat1-114 mutants and found that, meiotic nuclear divisions did not occur in the pef1Δ pat1-114 strain. Deletion of pef1 also suppressed the expression of DNA replication factors and the phosphorylation of Cdc2 Tyr-15. The double deletion of clg1 and psl1 arrested meiotic initiation in pat1-114 mutant cells, similar to that of pef1-deficient cells. Meiotic progression was also slightly delayed in the pas1-deficient strain. Our results reveal that Pef1 regulates cyclin-coordinated meiotic progression.

  Corresponding, Jan. 2021, Biomolecules, 11(1) (1), English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• LY6D-induced macropinocytosis as a survival mechanism of senescent cells.

  Taiki Nagano, Tetsushi Iwasaki, Kengo Onishi, Yuto Awai, Anju Terachi, Shione Kuwaba, Shota Asano, Ryoko Katasho, Kiyoko Nagai, Akio Nakashima, Ushio Kikkawa, Shinji Kamada

  Although senescent cells display various morphological changes including vacuole formation, it is still unclear how these processes are regulated. We have recently identified the gene, lymphocyte antigen 6 complex, locus D (LY6D), to be upregulated specifically in senescent cells. LY6D is a glycosylphosphatidylinositol-anchored cell-surface protein whose function remains unknown. Here, we analyzed the functional relationship between LY6D and the senescence processes. We found that overexpression of LY6D induced vacuole formation and knockdown of LY6D suppressed the senescence-associated vacuole formation. The LY6D-induced vacuoles were derived from macropinocytosis, a distinct form of endocytosis. Furthermore, Src family kinases and Ras were found to be recruited to membrane lipid rafts in an LY6D-dependent manner, and inhibition of their activity impaired the LY6D-induced macropinocytosis. Finally, reduction of senescent-cell survival induced by glutamine deprivation was recovered by albumin supplementation to the culture media in an LY6D-dependent manner. Because macropinocytosis acts as an amino acid supply route, these results suggest that LY6D-mediated macropinocytosis contributes to senescent-cell survival through the incorporation of extracellular nutrients.

  Nov. 2020, The Journal of biological chemistry, 296, 100049 - 100049, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The S. pombe CDK5 ortholog Pef1 regulates sexual differentiation through control of the TORC1 pathway and autophagy.

  Shinya Matsuda, Ushio Kikkawa, Haruka Uda, Akio Nakashima

  In Schizosaccharomyces pombe, a general strategy for survival in response to environmental changes is sexual differentiation, which is triggered by TORC1 inactivation. However, mechanisms of TORC1 regulation in fission yeast remain poorly understood. In this study, we found that Pef1, which is an ortholog of mammalian CDK5, regulates the initiation of sexual differentiation through positive regulation of TORC1 activity. Conversely, deletion of pef1 leads to activation of autophagy and subsequent excessive TORC1 reactivation during the early phases of the nitrogen starvation response. This excessive TORC1 reactivation results in the silencing of the Ste11-Mei2 pathway and mating defects. Additionally, we found that pef1 genetically interacts with tsc1 and tsc2 for TORC1 regulation, and physically interacts with three cyclins, Clg1, Pas1 and Psl1. The double deletion of clg1 and pas1 promotes activation of autophagy and TORC1 during nitrogen starvation, similar to what is seen in pef1Δ cells. Overall, our work suggests that Pef1-Clg1 and Pef1-Pas1 complexes regulate initiation of sexual differentiation through control of the TSC-TORC1 pathway and autophagy.

  Corresponding, Sep. 2020, Journal of cell science, 133(17) (17), English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• mTORC1 is involved in DGKβ-induced neurite outgrowth and spinogenesis.

  Hiroko Nakai, Ryosuke Tsumagari, Kenta Maruo, Akio Nakashima, Ushio Kikkawa, Shuji Ueda, Minoru Yamanoue, Naoaki Saito, Nobuyuki Takei, Yasuhito Shirai

  Diacylglycerol kinase β (DGKβ) is an enzyme converting DG to phosphatidic acid (PA) and is specifically expressed in neurons, especially those in the cerebral cortex, hippocampus and striatum. We previously reported that DGKβ induces neurite outgrowth and spinogenesis, contributing to higher brain function including emotion and memory, and plasma membrane localization of DGKβ via the C1 domain and a cluster of basic amino acids at the C-terminus is necessary for its function. To clarify the mechanisms involved in neuronal development by DGKβ, we investigated whether DGKβ activity induces neurite outgrowth using human neuroblastoma SH-SY5Y cells. DGKβ induced neurite outgrowth by activation of mammalian target of rapamycin complex 1 (mTORC1) through a kinase-dependent pathway. In addition, in primary cultured cortical and hippocampal neurons, inhibition of mTORC1 abolished DGKβ induced-neurite outgrowth, branching and spinogenesis. These results indicated that DGKβ induces neurite outgrowth and spinogenesis by activating mTORC1 in a kinase-dependent pathway.

  Mar. 2020, Neurochemistry international, 134, 104645 - 104645, English, International magazine

  [Refereed]

  Scientific journal


• TORC1 regulates autophagy induction in response to proteotoxic stress in yeast and human cells.

  Kazuki Suda, Atsuki Kaneko, Mitsugu Shimobayashi, Akio Nakashima, Tatsuya Maeda, Michael N Hall, Takashi Ushimaru

  Misfolded and aggregated proteins are eliminated to maintain protein homeostasis. Autophagy contributes to the removal of protein aggregates. However, if and how proteotoxic stress induces autophagy is poorly understood. Here we show that proteotoxic stress after treatment with azetidine-2-carboxylic acid (AZC), a toxic proline analog, induces autophagy in budding yeast. AZC treatment attenuated target of rapamycin complex 1 (TORC1) activity, resulting in the dephosphorylation of Atg13, a key factor of autophagy. By contrast, AZC treatment did not affect target of rapamycin complex 2 (TORC2). Proteotoxic stress also induced TORC1 inactivation and autophagy in fission yeast and human cells. This study suggested that TORC1 is a conserved key factor to cope with proteotoxic stress in eukaryotic cells.

  Apr. 2019, Biochemical and biophysical research communications, 511(2) (2), 434 - 439, English, International magazine

  Scientific journal


• D-amino acid oxidase promotes cellular senescence via the production of reactive oxygen species

  Taiki Nagano, Shunsuke Yamao, Anju Terachi, Hidetora Yarimizu, Haruki Itoh, Ryoko Katasho, Kosuke Kawai, Akio Nakashima, Tetsushi Iwasaki, Ushio Kikkawa, Shinji Kamada

  Jan. 2019, Life Science Alliance, 2(1) (1), e201800045, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Proline dehydrogenase promotes senescence through the generation of reactive oxygen species

  Taiki Nagano, Akio Nakashima, Kengo Onishi, Kosuke Kawai, Yuto Awai, Mizuki Kinugasa, Tetsushi Iwasaki, Ushio Kikkawa, Shinji Kamada

  Apr. 2017, JOURNAL OF CELL SCIENCE, 130(8) (8), 1413 - 1420, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Overexpression of eIF5 or its protein mimic 5MP perturbs eIF2 function and induces ATF4 translation through delayed re-initiation

  Caitlin Kozel, Brytteny Thompson, Samantha Hustak, Chelsea Moore, Akio Nakashima, Chingakham Ranjit Singh, Megan Reid, Christian Cox, Evangelos Papadopoulos, Rafael E. Luna, Abbey Anderson, Hideaki Tagami, Hiroyuki Hiraishi, Emily Archer Slone, Ken-ichi Yoshino, Masayo Asano, Sarah Gillaspie, Jerome Nietfeld, Jean-Pierre Perchellet, Stefan Rothenburg, Hisao Masai, Gerhard Wagner, Alexander Beeser, Ushio Kikkawa, Sherry D. Fleming, Katsura Asano

  Oct. 2016, NUCLEIC ACIDS RESEARCH, 44(18) (18), 8704 - 8713, English

  [Refereed]

  Scientific journal


• Identification of cellular senescence-specific genes by comparative transcriptomics

  Taiki Nagano, Masayuki Nakano, Akio Nakashima, Kengo Onishi, Shunsuke Yamao, Masato Enari, Ushio Kikkawa, Shinji Kamada

  Aug. 2016, SCIENTIFIC REPORTS, 6, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The Loss of Lam2 and Npr2-Npr3 Diminishes the Vacuolar Localization of Gtr1-Gtr2 and Disinhibits TORC1 Activity in Fission Yeast

  Ning Ma, Yan Ma, Akio Nakashima, Ushio Kikkawa, Tomoyuki Furuyashiki

  May 2016, PLOS ONE, 11(5) (5), English

  [Refereed]

  Scientific journal


• Versatile function of the circadian protein CIPC as a regulator of Erk activation

  Ryota Matsunaga, Tasuku Nishino, Atsushi Yokoyama, Akio Nakashima, Ushio Kikkawa, Hiroaki Konishi

  Jan. 2016, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 469(3) (3), 377 - 383, English

  [Refereed]

  Scientific journal


• Midostaurin preferentially attenuates proliferation of triple-negative breast cancer cell lines through inhibition of Aurora kinase family

  Masaaki Kawai, Akio Nakashima, Shinji Kamada, Ushio Kikkawa

  Jul. 2015, JOURNAL OF BIOMEDICAL SCIENCE, 22(1) (1), English

  [Refereed]

  Scientific journal


• Fission yeast arrestin-related trafficking adaptor, Arn1/Any1, is ubiquitinated by Pub1 E3 ligase and regulates endocytosis of Cat1 amino acid transporter

  Akio Nakashima, Shinji Kamada, Fuyuhiko Tamanoi, Ushio Kikkawa

  Jun. 2014, BIOLOGY OPEN, 3(6) (6), 542 - 552, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Identification of Regions Critical for the Integrity of the TSC1-TSC2-TBC1D7 Complex

  Arthur Jorge Santiago Lima, Marianne Hoogeveen-Westerveld, Akio Nakashima, Anneke Maat-Kievit, Ans van den Ouweland, Dicky Halley, Ushio Kikkawa, Mark Nellist

  Apr. 2014, PLOS ONE, 9(4) (4), English

  [Refereed]

  Scientific journal


• Branched-Chain Amino Acids Enhance Premature Senescence through Mammalian Target of Rapamycin Complex I-Mediated Upregulation of p21 Protein

  Masayuki Nakano, Akio Nakashima, Taiki Nagano, Shintaro Ishikawa, Ushio Kikkawa, Shinji Kamada

  Nov. 2013, PLOS ONE, 8(11) (11), English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A positive role of mammalian Tip41-like protein, TIPRL, in the amino-acid dependent mTORC1-signaling pathway through interaction with PP2A

  Akio Nakashima, Keiko Tanimura-Ito, Noriko Oshiro, Satoshi Eguchi, Takafumi Miyamoto, Ayaka Momonami, Shinji Kamada, Kazuyoshi Yonezawa, Ushio Kikkawa

  Lead, Sep. 2013, FEBS LETTERS, 587(18) (18), 2924 - 2929, English

  [Refereed]

  Scientific journal


• Cyclin I is involved in the regulation of cell cycle progression

  Taiki Nagano, Toshiaki Hashimoto, Akio Nakashima, Shin-ichi Hisanaga, Ushio Kikkawa, Shinji Kamada

  Aug. 2013, CELL CYCLE, 12(16) (16), 2617 - 2624, English

  [Refereed]

  Scientific journal


• Association of CAD, a multifunctional protein involved in pyrimidine synthesis, with mLST8, a component of the mTOR complexes

  Akio Nakashima, Ippei Kawanishi, Sumiko Eguchi, Eugene Hsin Yu, Satoshi Eguchi, Noriko Oshiro, Ken-ichi Yoshino, Ushio Kikkawa, Kazuyoshi Yonezawa

  Lead, Apr. 2013, JOURNAL OF BIOMEDICAL SCIENCE, 20(1) (1), English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Psk1, an AGC kinase family member in fission yeast, is directly phosphorylated and controlled by TORC1 and functions as S6 kinase

  Akio Nakashima, Yoko Otsubo, Akira Yamashita, Tatsuhiro Sato, Masayuki Yamamoto, Fuyuhiko Tamanoi

  Lead, Dec. 2012, JOURNAL OF CELL SCIENCE, 125(23) (23), 5840 - 5849, English

  [Refereed]

  Scientific journal


• X-linked inhibitor of apoptosis protein mediates neddylation by itself but does not function as a NEDD8-E3 ligase for caspase-7

  Taiki Nagano, Toshiaki Hashimoto, Akio Nakashima, Ushio Kikkawa, Shinji Kamada

  Jun. 2012, FEBS LETTERS, 586(11) (11), 1612 - 1616, English

  [Refereed]

  Scientific journal


• Preferential Fas-mediated apoptotic execution at G(1) phase: the resistance of mitotic cells to the cell death

  T. Hashimoto, K. Juso, M. Nakano, T. Nagano, S. Kambayashi, A. Nakashima, U. Kikkawa, S. Kamada

  May 2012, CELL DEATH & DISEASE, 3(5) (5), English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Quantitative 3D imaging of whole, unstained cells by using X-ray diffraction microscopy

  Huaidong Jiang, Changyong Song, Chien-Chun Chen, Rui Xu, Kevin S. Raines, Benjamin P. Fahimian, Chien-Hung Lu, Ting-Kuo Lee, Akio Nakashima, Jun Urano, Tetsuya Ishikawa, Fuyuhiko Tamanoi, Jianwei Miao

  Jun. 2010, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107(25) (25), 11234 - 11239, English

  [Refereed]

  Scientific journal


• Single amino-acid changes that confer constitutive activation of mTOR are discovered in human cancer

  T. Sato, A. Nakashima, L. Guo, K. Coffman, F. Tamanoi

  May 2010, ONCOGENE, 29(18) (18), 2746 - 2752, English

  Scientific journal


• Fission yeast TORC1 regulates phosphorylation of ribosomal S6 proteins in response to nutrients and its activity is inhibited by rapamycin

  Akio Nakashima, Tatsuhiro Sato, Fuyuhiko Tamanoi

  Mar. 2010, JOURNAL OF CELL SCIENCE, 123(5) (5), 777 - 786, English

  Scientific journal


• [Rheb-mTOR signaling pathway involved in tumor formation].

  Sato T, Nakashima A, Tamanoi F

  1, Jan. 2010, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 55(1) (1), 11 - 17, Japanese

  [Refereed]


• Specific Activation of mTORC1 by Rheb G-protein in Vitro Involves Enhanced Recruitment of Its Substrate Protein

  Tatsuhiro Sato, Akio Nakashima, Lea Guo, Fuyuhiko Tamanoi

  May 2009, JOURNAL OF BIOLOGICAL CHEMISTRY, 284(19) (19), 12783 - 12791, English

  Scientific journal


• The Yeast Tor Signaling Pathway Is Involved in G2/M Transition via Polo-Kinase

  Akio Nakashima, Yoshiko Maruki, Yuko Imamura, Chika Kondo, Tomoko Kawamata, Ippei Kawanishi, Hideki Takata, Akira Matsuura, Kyung S. Lee, Ushio Kikkawa, Yoshinori Ohsumi, Kazuyoshi Yonezawa, Yoshiaki Kamada

  May 2008, PLOS ONE, 3(5) (5), English

  [Refereed]

  Scientific journal


• AMP-activated protein kinase phosphorylates Golgi-specific brefeldin A resistance factor 1 at Thr(1337) to induce disassembly of Golgi apparatus

  Takafumi Miyamoto, Noriko Oshiro, Ken-Ichi Yoshino, Akio Nakashima, Satoshi Eguchi, Mikiko Takahashi, Yoshitaka Ono, Ushio Kikkawa, Kazuyoshi Yonezawa

  Feb. 2008, JOURNAL OF BIOLOGICAL CHEMISTRY, 283(7) (7), 4430 - 4438, English

  Scientific journal


• Identification of TBC7 having TBC domain as a novel binding protein to TSC1-TSC2 complex

  Akio Nakashima, Ken-ichi Yoshino, Takafumi Miyamoto, Satoshi Eguchi, Noriko Oshiro, Ushio Kikkawa, Kazuyoshi Yonezawa

  Sep. 2007, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 361(1) (1), 218 - 223, English, International magazine

  [Refereed]

  Scientific journal


• The proline-rich akt substrate of 40 kDa (PRAS40) is a physiological substrate of mammalian target of rapamycin complex 1

  Noriko Oshiro, Rinako Takahashi, Ken-ichi Yoshino, Keiko Tanimura, Akio Nakashima, Satoshi Eguchi, Takafumi Miyamoto, Kenta Hara, Kenji Takehana, Joseph Avruch, Ushio Kikkawa, Kazuyoshi Yonezawa

  Jul. 2007, JOURNAL OF BIOLOGICAL CHEMISTRY, 282(28) (28), 20329 - 20339, English

  Scientific journal


• A starvation-specific serine protease gene, isp6(+), is involved in both autophagy and sexual development in Schizosaccharomyces pombe

  Akio Nakashima, Takahiro Hasegawa, Saori Mori, Masaru Ueno, Shigeyasu Tanaka, Takashi Ushimaru, Shusei Sato, Masahiro Uritani

  Jun. 2006, CURRENT GENETICS, 49(6) (6), 403 - 413, English

  [Refereed]

  Scientific journal


• Suppression of the mTOR-raptor signaling pathway by the inhibitor of heat shock protein 90 geldanamycin

  G Ohji, S Hidayat, A Nakashima, C Tokunaga, N Oshiro, K Yoshino, K Yokono, U Kikkawa, K Yonezawa

  Jan. 2006, JOURNAL OF BIOCHEMISTRY, 139(1) (1), 129 - 135, English

  Scientific journal


• Involvement of a CCAAT-binding complex in the expression of a nitrogen-starvation-specific gene, isp6(+), in Schizosaccharomyces pombe

  A Nakashima, M Ueno, T Ushimaru, M Uritani

  Oct. 2002, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 66(10) (10), 2224 - 2227, English

  [Refereed]

  Scientific journal


• Genes for a nuclease and a protease are involved in the drastic decrease in cellular RNA amount in fission yeast cells during nitrogen starvation

  A Nakashima, M Yoshida, K Nakayama, A Kato-Furuno, M Ueno, T Ushimaru, M Uritani

  Mar. 2002, JOURNAL OF BIOCHEMISTRY, 131(3) (3), 391 - 398, English

  [Refereed]

  Scientific journal


• Fission yeast Tor1 functions in response to various stresses including nitrogen starvation, high osmolarity, and high temperature

  M Kawai, A Nakashima, M Ueno, T Ushimaru, K Aiba, H Doi, M Uritani

  May 2001, CURRENT GENETICS, 39(3) (3), 166 - 174, English

  [Refereed]

  Scientific journal

• TORC1-dependent phosphorylation targets in fission yeast

  Yoko Otsubo, Akio Nakashima, Masayuki Yamamoto, Akira Yamashita

  MDPI AG, 01 Sep. 2017, Biomolecules, 7(3) (3), e50, English

  [Refereed][Invited]

  Book review

  Link to Kobe Univ. Repository (Kernel)


• mTOR is involved in both kinase activity-dependent and -independent pathways of DGK beta-regulated neurite and branch induction

  Takuya Kano, Hiroko Nakai, Akio Nakashima, Ushio Kikkawa, Naoaki Saito, Nobuyuki Takei, Shuji Ueda, Minoru Yamanoue, Yasuhito Shirai

  2014, JOURNAL OF PHARMACOLOGICAL SCIENCES, 124, 100P - 100P, English

  Summary international conference


• Involvement of mTOR in diacylglycerol kinase beta (DGK beta)-induced neurite blanching and spine formation

  H. Nakai, N. Takei, A. Nakashima, U. Kikkawa, N. Saito, S. Ueda, M. Yamanoue, Y. Shirai

  Sep. 2012, FEBS JOURNAL, 279, 383 - 384, English

  Summary international conference


• アミノ酸センシングシステムとしてのmTOR複合体１の機序

  原賢太, NAKASHIMA AKIO, 吉川潮

  羊土社, 2011, 実験医学, 29, 865 - 870, Japanese

  [Invited]

  Introduction commerce magazine


• Conservation of the Tsc/Rheb/TORC1/S6K/S6 signaling in fission yeast

  Akio Nakashima, Fuyuhiko Tamanoi

  ELSEVIER, 2010, Enzymes, 28(C) (C), 167 - 187, English

  [Invited]

  Others


• Rheb-mTORシグナルと細胞がん化への関与

  佐藤龍洋, NAKASHIMA AKIO, 玉野井冬彦

  共立出版, Jan. 2010, 蛋白質 核酸 酵素, 55(1) (1), 11 - 17, Japanese

  [Invited]

  Introduction commerce magazine


• TSC1-TSC2複合体の新しい結合タンパク質TBC7

  NAKASHIMA AKIO

  医学書院, 2008, 生体と科学, 59(6) (6), 497 - 503, Japanese

  [Invited]

  Introduction commerce magazine


• 細胞環境中のアミノ酸バランスを感知するmTORシグナル伝達機構

  NAKASHIMA AKIO, 米澤 一仁

  北隆館, 2004, BIO Clinica, 19, 18 - 22, Japanese

  [Invited]

  Introduction commerce magazine


• Function of a fission yeast RNA/DNA degrading enzyme in nitrogen starvation response

  YOSHIDA Mayumi, NAKASHIMA Akio, USHIMARU Takashi, UENO Masaru, URITANI Masahiro

  01 Dec. 1998, 日本分子生物学会年会プログラム・講演要旨集, 21, 581 - 581, Japanese

• The Enzymes

  NAKASHIMA Akio

  Joint work, Conservation of the Tsc/Rheb/TORC1/S6K/S6 signaling in fission yeast, 2010


• Rheb-mTORシグナルと細胞がん化への関与/蛋白質 核酸 酵素

  佐藤 龍洋, NAKASHIMA AKIO, 玉野井 冬彦

  Joint work, 共立出版, Jan. 2010, Japanese

  Scholarly book


• BIO Clinica Vol. 19 / 細胞環境中のアミノ酸バランスを感知するmTORシグナル伝達機構

  中嶋 昭雄, YONEZAWA, Kazuyoshi

  Joint work, 北陸館, 2004, Japanese

  Scholarly book

• 減数分裂過程におけるオートファジーの制御機構及び生理的役割

  門脇 有哉, 宇田 晴香, 谷本 茉結, 宮迫 剛士, 山下紗夕美, 中嶋昭雄

  第46回日本分子生物学会年会, Japanese

  Poster presentation


• 分裂酵母の減数分裂におけるTORC1とオートファジーの制御

  中嶋昭雄, 門脇 有哉, 宇田晴香, 山下紗夕美, 三笠瑛里華, 杉原真子

  第13回TOR研究会, Japanese

  Oral presentation


• 分裂酵母TORC1の減数分裂過程における制御機構

  中嶋昭雄, 三笠瑛里華, 杉原真子, 岡久萌菜, 宮本由衣, 松田真弥

  第56回酵母遺伝学フォーラム, Japanese

  Poster presentation


• 分裂酵母の減数分裂におけるオートファジー制御

  門脇 有哉, 山下 紗夕美, 宇田 晴香, 中嶋昭雄

  第56回酵母遺伝学フォーラム, Japanese

  Poster presentation


• 分裂酵母おける減数分裂進行に伴うオートファジーの発生と役割について

  山下 紗夕美, 宇田 晴香, 中嶋 昭雄, 神戸大, 院農, 資源生命科学, 神戸大, バイオシグナル

  第45回日本分子生物学会年会, Dec. 2022, Japanese

  Poster presentation


• 分裂酵母の減数分裂におけるTORC1活性とオートファジー

  中嶋 昭雄, 宇田 晴香, 山下 紗夕美, 神戸大, 院農, 資源生命科学, 神戸大, バイオシグナル

  第１２回TOR研究会, Oct. 2022, Japanese

  Oral presentation


• Regulation of autophagy during meiosis in Schizosaccharomyces pombe

  Haruka Uda, Sayumi Yamashita, Akio Nakashima

  第44回日本分子生物学会年会，パシフィコ横浜, Dec. 2021, Japanese

  Poster presentation


• 分裂酵⺟の減数分裂におけるTORC1活性とオートファジー

  宇田晴香, 山下紗夕美, 吉川 潮, 中嶋昭雄

  第11回TOR研究会, Jul. 2021, Japanese

  Oral presentation


• 分裂酵母サイクリン依存性キナーゼPef1はTORC１を介して減数分裂の開始・進行を制御する

  松田真弥, 岡久萌菜, 宮本由衣, 吉川 潮, 中嶋昭雄

  第４２回日本分子生物学会年会, Dec. 2019, Japanese

  Poster presentation


• 分裂酵母Pef1/TORC1シグナル伝達機構の解析

  松田真弥, 岡久萌菜, 宮本由衣, 吉川潮, 中嶋昭雄

  酵母遺伝学フォーラム第52回研究報告，静岡市清水文化会館マリナート, Sep. 2019, Japanese

  Oral presentation


• 分裂酵母Pef1/CDK5によるTORC1制御機構

  MATSUDA SHINYA, OKAHISA MOENA, MIYAMOTO YUI, KIKKAWA USHIO, NAKASHIMA AKIO

  第９回TOR研究会, Jun. 2019, Japanese, 久留米シティプラザ 久留米市, Domestic conference

  Oral presentation


• 有性生殖過程における分裂酵母Pef1(CDK5)/TORC1シグナル伝達機構の解析

  Matsuda Shinya, KIKKAWA USHIO, NAKASHIMA AKIO

  第41回日本分子生物学会年会, Nov. 2018, Japanese, 横浜市，パシフィコ横浜, Domestic conference

  Poster presentation


• LY6Dは老化細胞の細胞膜脂質ラフト上でSrcやRasと会合することによりマクロピノサイトーシスを誘導する

  長野 太輝, 岩崎 哲史, 寺地 杏樹, 麻野 翔太, 片所 諒子, 長井 清香, 中嶋 昭雄, 吉川 潮, 鎌田 真司

  第41回日本分子生物学会年会, Nov. 2018, Japanese, Domestic conference

  Poster presentation


• 分裂酵母Pef1/CDK5はTORC1を介して有性生殖過程の開始・進行を制御する

  Matsuda Shinya, KIKKAWA USHIO, NAKASHIMA AKIO

  酵母遺伝学フォーラム第51回研究報告会, Sep. 2018, Japanese, 福岡市，九州大学医学部百年講堂, Domestic conference

  Poster presentation


• 分裂酵母Pef1/CDK5はTORC1制御に関与する？

  Matsuda Shinya, Kikkawa Ushio, NAKASHIMA AKIO

  第８回TOR研究会, Jun. 2018, Japanese, 蛋白研セミナー“TORを介した細胞成長統御の総合的理解”, 大阪市，大阪大学中之島センター, Domestic conference

  Oral presentation


• 減数分裂における分裂酵母TORC1の制御

  NAKASHIMA AKIO, Yamashita Akira, Otsubo Yoko, Matsuda Shinya, Kamada Shinji, Uritani Masahiro, Yamamoto Masayuki, Kikkawa Ushio

  ConBio2017（生命科学系学会合同年次大会）, Dec. 2017, Japanese, 神戸ポートアイランド, Domestic conference

  [Invited]

  Invited oral presentation


• LY6Dにより誘導されるマクロピノサイトーシスは老化細胞の生存促進に働く

  長野 太輝, 大西 健悟, 岩崎 哲史, NAKASHIMA AKIO, 吉川 潮, 鎌田 真司

  ConBio2017, Dec. 2017, Japanese, 神戸, Domestic conference

  Oral presentation


• モデル生物を用いたTORC1 の減数分裂における制御と機能

  NAKASHIMA AKIO

  第四回バイオシグナル研究会「栄養シグナリングTOR と細胞機能制御〜基礎研究から創薬へ〜」, Sep. 2017, Japanese, 神戸大学瀧川記念学術交流会館, Domestic conference

  [Invited]

  Invited oral presentation


• p53によるアミノ酸代謝経路の調節が細胞老化を誘導する

  長野 太輝, 山尾 俊介, 中嶋 昭雄, 岩崎 哲史, 吉川 潮, 鎌田 真司

  日本分子生物学会, Nov. 2016, Japanese, 横浜, Domestic conference

  Public symposium


• フェロモン応答シグナルによる分裂酵母TORC1制御の可能性

  NAKASHIMA AKIO, KIKKAWA USHIO

  第６回TOR研究会, Sep. 2016, Japanese, 東京大学 分子細胞生物学研究所, Domestic conference

  Oral presentation


• Regulation and function of TORC1 signaling in meiosis under nitrogen starvation

  NAKASHIMA AKIO, Yamashita Akira, Otsubo Yoko, Kamada Shinji, Uritani Masahiro, Yamamoto Masayuki, 吉川 潮

  Biochemistry and Molecular Biology 2015, Dec. 2015, Japanese, 特定非営利活動法人 日本分子生物学会, 神戸ポートアイランド, Domestic conference

  [Invited]

  Invited oral presentation


• Regulation of TORC1 signaling in meiosis under nitrogen starvation

  NAKASHIMA AKIO, Yamashita Akira, Otsubo Yoko, Kamada Shinji, Uritani Masahiro, Yamamoto Masayuki, Kikkawa Ushio

  THE EIGHTH INTERNATIONAL FISSION YEAST MEETING, Jun. 2015, English, Kobe, International conference

  Poster presentation


• 新規細胞老化誘導因子としてのDアミノ酸酸化酵素DAOの機能解析

  山尾 俊介, 長野 太輝, NAKASHIMA AKIO, KIKKAWA USHIO, KAMADA SHINJI

  第37回日本分子生物学会, Nov. 2014, Japanese, 横浜, Domestic conference

  Poster presentation


• プロリン脱水素酵素（PRODH）は細胞老化誘導に関わる新規因子である

  長野 太輝, 山尾 俊介, NAKASHIMA AKIO, KIKKAWA USHIO, KAMADA SHINJI

  第37回日本分子生物学会年会, Nov. 2014, Japanese, パシフィコ横浜, Domestic conference

  Poster presentation


• 分裂酵母 TORC1 の窒素源飢餓時の再活性化と生理機能

  NAKASHIMA AKIO, KIKKAWA USHIO

  第47回酵母遺伝学フォーラム報告会, Sep. 2014, Japanese, 東京大学農学部弥生キャンパス東京大学弥生講堂, Domestic conference

  Poster presentation


• 多機能性 TORC1 キナーゼの液胞依存性栄養シグナルによる活性制御システムの解明

  NAKASHIMA AKIO

  第186回酵母細胞研究会例会 平成24年度地神芳文記念研究助成金研究成果報告会, Jul. 2014, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• 栄養枯渇時の分裂酵母TORC1の活性と機能

  NAKASHIMA AKIO, KIKKAWA USHIO

  第36回日本分子生物学会年会, Dec. 2013, Japanese, 日本分子生物学会, Domestic conference

  Oral presentation


• 分裂酵母TORC1の窒素源飢餓時の再活性化と生理機能の検討

  NAKASHIMA AKIO, KIKKAWA USHIO

  第46回酵母遺伝学フォーラム年会, Sep. 2013, Japanese, 酵母遺伝学フォーラム, Domestic conference

  Oral presentation


• 分裂酵母アレスチン様タンパク質Arn1とそのユビキチン化を介したアミノ酸の細胞内取り込みの制御

  NAKASHIMA AKIO, 玉野井冬彦, KIKKAWA USHIO

  第35回日本分子生物学会年会, Dec. 2012, Japanese, 日本分子生物学会, Domestic conference

  Poster presentation


• アミノ酸トランスポーターCat1のアレスチン様タンパク質による機能制御

  NAKASHIMA AKIO, 玉野井冬彦, KIKKAWA USHIO

  第45回酵母遺伝学フォーラム年会, Sep. 2012, Japanese, 酵母遺伝学フォーラム, Domestic conference

  Poster presentation


• Molecular mechanism of DGKβ- mediated neurite blanching and spine formation

  NAKAI Hiroko, TAKEI Nobuyuki, NAKASHIMA Akio, KIKKAWA USHIO, SAITO Naoaki, UEDA Shuji, YAMANOUE Minoru, SHIRAI Yasuhito

  The 35th Annual Meeting of the Japan Neuroscience Society, Sep. 2012, Japanese, Japan Neuroscience Society, Nagoya Congress Center, Domestic conference

  Poster presentation


• Involvement of mTOR in Diacylglycerol kinase β (DGKβ)-induced neurite branching and spine formation.

  NAKAI Hiroko, NAKASHIMA Akio, KIKKAWA USHIO, SAITO Naoaki, TAKEI Nobuyuki, UEDA Shuji, YAMANOUE Minoru, SHIRAI Yasuhito

  The 22nd IUBMB & the 37th FEBS, Sep. 2012, English, IUBMB & FEBS, Sevilla, Spain, International conference

  Poster presentation


• ジアシルグリセロールキナーゼ_による特徴的な神経突起伸にはmTORが関与する

  SHIRAI Yasuhito, NAKAI Hiroko, NAKANISHI Hiroki, NAKASHIMA Akio, KIKKAWA Ushio, UEDA Shuji, YAMANOUE Minoru, SAITO Naoaki

  第54回日本脂質生化学会大会, Jun. 2012, Japanese, 日本脂質生化学会, 九州大学, Domestic conference

  Oral presentation


• Mammalian TIP41-like protein, mTIP41, as a positive regulator of the amino acid-stimulated mTORC1 signaling.

  Akio Nakashima, Ayaka Monmonami, Noriko Oshiro, Ushio Kikkawa

  第34回日本分子生物学会年会, Dec. 2011, English, 日本分子生物学会, Domestic conference

  Oral presentation


• アレスチン様タンパク質Art1によるアミノ酸透過酵素Cat1の機能解析

  NAKASHIMA AKIO, KIKKAWA USHIO, 玉野井冬彦

  第44回酵母遺伝学フォーラム年会, Sep. 2011, Japanese, 酵母遺伝学フォーラム, Domestic conference

  Poster presentation


• 分裂酵母TORC1によるS6キナーゼPsk1の制御

  NAKASHIMA AKIO, 大坪瑤子, 山下朗, 山本正幸, 玉野井冬彦

  BMB2010（日本生化学会・日本分子生物学会合同大会）, Dec. 2010, Japanese, 日本分子生物学会, Domestic conference

  Oral presentation


• 分裂酵母TORC1による栄養依存的なリボソームタンパクS6のリン酸化制御

  NAKASHIMA AKIO, 玉野井冬彦

  第43回酵母遺伝学フォーラム年会, Sep. 2010, Japanese, 酵母遺伝学フォーラム, Domestic conference

  Oral presentation


• THE TORC1-S6K-S6 SIGNALING IN FISSION YEAST.

  Nakashima Akio, Tamanoi Fuyuhiko

  The 5th international fission yeast meeting, 2009, English, International conference

  Poster presentation

• 酵母遺伝学フォーラム


• 日本分子生物学会

• Establishment and spread of the new assay for sperm flagellar function to improve artificial insemination results in the cattle

  原山 洋, 中嶋 昭雄

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Kobe University, 01 Apr. 2022 - 31 Mar. 2025


• 減数分裂におけるTORC1キナーゼおよびオートファジーの機能制御の分子機構

  中嶋 昭雄

  日本学術振興会, 科学研究費助成事業 基盤研究(C), 基盤研究(C), 神戸大学, 01 Apr. 2022 - 31 Mar. 2025


• Analyses of 3-dimensional rotation for the purpose of reconsidering criteria for the evaluation of flagellar functions in bull sperm

  原山 洋, 中嶋 昭雄, 坂瀬 充洋

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Challenging Research (Exploratory), Challenging Research (Exploratory), Kobe University, 28 Jun. 2019 - 31 Mar. 2021


• ウシ凍結前精子の耐凍能判定法の確立を目的としたSPACA1タンパク質の解析と利用

  原山 洋

  学術研究助成基金助成金／基盤研究(C), Apr. 2016 - Mar. 2019

  Competitive research funding


• モデル生物を用いた減数分裂期におけるTORC1の活性化と生理機能の分子機構の解明

  中嶋 昭雄

  学術研究助成基金助成金／基盤研究(C), Apr. 2014 - Mar. 2017, Principal investigator

  Competitive research funding


• 分裂酵母ＴＯＲＣ１を介した栄養感知・細胞制御シグナルの分子メカニズムの構築

  中嶋 昭雄

  科学研究費補助金／若手研究(B), Apr. 2011 - Mar. 2013, Principal investigator

  Competitive research funding


• ｍＴＯＲを介する栄養シグナリングのタンパク質ホスファターゼＰＰ２Ａによる制御機構

  吉川 潮

  科学研究費補助金／基盤研究(B), 2010

  Competitive research funding


• 分裂酵母のストレス適応機構の研究.

  Competitive research funding


• ストレス適応の分子メカニズムの研究

  Competitive research funding


• TORを介する栄養シグナリングの解明と細胞制御機構の解析

  Competitive research funding


• Research on the molecular mechanism of the stress-response.

  Competitive research funding


• Study of the stress-response mechanism in fission yeast.

  Competitive research funding