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IKEDA Kenichi
Graduate School of Agricultural Science / Department of Agrobioscience
Associate Professor

Researcher basic information

■ Research Keyword
  • cell biology
  • plant pathology
■ Research Areas
  • Environmental science/Agricultural science / Conservation science (plants)
■ Committee History
  • バイオコントロール研究会, 幹事

Research activity information

■ Award
  • Jun. 2024 The Genetics Society of Thailand, Best Oral Presentation, Transcriptome analysis of Pyricularia oryzae high-temperature response during appressorium formation
    Thanathip Sutthiphai, Sayuki Hiraiwa, Hitoshi Nakayashiki, Kenichi Ikeda, Chatchawan Jantasuriyara

  • Mar. 2024 日本植物病理学会, Best Paper Award of Journal of General Plant Pathology, Japanese star anise ringspot-associated virus is a distinct emaravirus transmitted by the eriophyid mite (the family Diptilomoipidae)
    Yoshifumi Shimomoto, Tomoyuki Okada, Kenichi Ikeda, Akio Tatara, Yusuke Hasegawa, Hironobu Yanagisawa, Sawana Takeyama, Kazusa Hayashi, Kazutaka Yano, Yasuaki Morita, Kenji Kubota

  • Mar. 2018 日本植物病理学会, Journal of General Plant Pathology誌論文賞, Novel aspects of hydrophobins in wheat isolate of Magnaporthe oryzae: Mpg1, but not Mhp1, is essential for adhesion and pathogenicity
    井上 加奈子, 北岡 治正, PARK Pyoyun, IKEDA KENICHI
    Official journal

  • Aug. 2015 日本植物病理学会, 植物感染生理談話会ポスター発表優秀賞, Ralstonia solanacearum OE1-1株のバイオフィルム形成にレクチンES-IILは必要である
    森 友花, 井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 大西 秀幸, 甲斐 建次, 木場 章範, 大西 浩平, 曳地 康史
    Japan society

  • Jun. 2015 医学生物学電子顕微鏡技術学会, 奨励賞, 電子顕微鏡分野における複数の優れた業績
    IKEDA KENICHI
    Official journal

  • Sep. 2014 日本育種学会, 第126回講演会日本育種学会優秀発表賞, 野生イネ(Oryza rufipogon)の遺伝的背景における種子脱粒性遺伝子座間の相互作用と離層形態
    井上 千鶴, Than Myint Htun, INOUE KANAKO, IKEDA KENICHI, ISHII TAKASHIGE, ISHIKAWA RYO
    Japan society

  • 2012 日本土壌微生物学会, 日本土壌微生物学会優秀ポスター賞, ゼラチナーゼ活性を有する土壌微生物を利用した植物病害防除法の開発
    北川博子, 井上加奈子, 下井沙紀, 朴杓允, IKEDA KENICHI
    Japan society

  • 2012 日本植物病理学会, The 2nd Korea-Japan Joint Symposium 2012 for Plant Pathology Excellent Poster Prize, Detachment control: importance of extracellular matrix (ECM) for pathogenicity and potential target for disease protection
    Kitagawa H, Inoue K, Shimoi S, Kitaoka H, Park P, IKEDA KENICHI
    International society

  • 2011 日本植物病理学会, 日本植物病理学会論文賞, Chuma, I., Shinogi, T., Hosogi, N., Ikeda, K., Nakayashiki, H., Park, P., and Tosa, Y. (2009) Cytological characteristics of microconidia of Magnaporthe oryzae. J. Gen. Plant Pathol. 75：353-358
    Chuma Izumi, Shinogi, T, Hosogi, N, Ikeda Kenichi, Nakayashiki Hitoshi, Park Pyoyun, TOSA YUKIO
    Official journal

  • 2006 日本植物病理学会, 日本植物病理学会学術奨励賞, 紋羽病菌におけるdsRNAの多様性とその動態
    IKEDA KENICHI
    Japan society

■ Paper
  • Atsumi Morimoto, Thach An Dang, Ken-Ichi Ikeda, Hitoshi Nakayashiki
    Centromeres are essential for chromosome segregation, yet they are among the most rapidly evolving regions of the genome. The mechanisms driving this rapid evolution of centromeric sequences are still not well understood. In this study, we identified the centromeric sequences of a wheat-infecting Pyricularia oryzae strain (Br48) using CENP-A chromatin immunoprecipitation followed by high-throughput sequencing. The Br48 centromeres range from 71 kb to 101 kb in length and are highly AT-rich (72.1-75.5%) and repeat-rich (63.4-85.0%). These regions are also enriched in H3K9me3 and 5-methylcytosine but depleted of H3K4me2 and H3K27me3. During the analysis of repetitive sequences in the Br48 centromere, we identified a stretch of approximately 530 bp that is tightly associated with centromeres in the Pyricularia fungus. We designated this element as the centromere-associated IR element (CenIR), as it often forms inverted repeat structures with two elements adjacent in reverse orientation. A comparison of putative centromere sequences across phylogenetically distinct P. oryzae strains suggests that changes in centromeric sequences are non-uniform across chromosomes and do not always align with the fungal phylogenetic relationships. Repeat-induced point mutation (RIP)-like CG to TA transitions likely accelerate base substitutions in the centromeres of Pyricularia fungi.
    Jan. 2025, Genes & genetic systems, English, Domestic magazine
    Scientific journal

  • Zikai Xiang, Hitoshi Nakayashiki, Kenichi Ikeda
    Elsevier BV, Dec. 2024, Fungal Biology, 101524 - 101524
    Scientific journal

  • Fumihiro Nishimura, Hiromitsu Furuya, Takahiro Katayama, Kenichi Ikeda
    The Kansai Plant Protection Society, Jun. 2024, Annual Report of The Kansai Plant Protection Society, 66, 55 - 60, Japanese
    [Refereed]
    Scientific journal

  • Ken-ichi Ikeda, Kaichi Uchihashi, Itsuki Okuda, Zikai Xiang, Hitoshi Nakayashiki
    Abstract Wheat blast is an important disease that affects cross-border food security. The causal pathogen, Pyricularia oryzae (syn. Magnaporthe oryzae) Triticum pathotype (MoT), forms a phylogenetic group distinct from the other P. oryzae pathotypes pathogenic on various gramineous plants. Here we developed MoT-specific primer sets for qPCR and LAMP detection methods, and their amplification specificity and efficiency were tested in comparison with previous reported MoT-specific primers. The amplification efficiency and specificity were affected by the polymerase premix. To evaluate the detection specificity, purified DNA samples of 15 MoT isolates, 89 non-MoT isolates of Pyricularia spp., and crude DNA from wheat plants inoculated with MoT were used. We conclude that the Sp3 primer sets for qPCR and the MoT3L primer sets for LAMP were the best in this research.
    Springer Science and Business Media LLC, Dec. 2023, Journal of General Plant Pathology, 90, 82 - 94, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Hideki Ishida, Kousei Yamamoto, Yuki Yano, Kenichi Ikeda, Liudmyla Gaponova, Rina Higuchi, Andrii Kolosiuk, Toshinobu Suzaki
    Water freeze-drying of biological samples has long been considered unfeasible as a sample preparation method for scanning electron microscopy (SEM) owing to the formation of damaging ice crystals. Contrary to this assumption, however, when live ciliates in water were frozen in contact with pre-cooled copper blocks in this study, they could be freeze-dried without artifact formation, although the success rate was only about 10%. This method offers several advantages over the traditional approach of chemical fixation followed by dehydration and drying. First, the degree of sample shrinkage associated with sample preparation was much lower than with chemical fixation. Second, contractile ciliates such as Spirostomum and Lacrymaria could be sampled in their elongated state, and the metachronal waves of cilia on the cell surface were well preserved. Moreover, this method requires no special equipment and only a few hours at most for sample preparation before SEM observation. Thus, although this method needs to be improved to increase the success rate in the future, it can be used to prepare samples for SEM observation that could not be prepared by other methods.
    Frontiers Media SA, Nov. 2023, Frontiers in Protistology, 1, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Jens H. Kuhn, Junya Abe, Scott Adkins, Sergey V. Alkhovsky, Tatjana Avšič-Županc, María A. Ayllón, Justin Bahl, Anne Balkema-Buschmann, Matthew J. Ballinger, Virendra Kumar Baranwal, Martin Beer, Nicolas Bejerman, Éric Bergeron, Nadine Biedenkopf, Carol D. Blair, Kim R. Blasdell, Arnaud G. Blouin, Steven B. Bradfute, Thomas Briese, Paul A. Brown, Ursula J. Buchholz, Michael J. Buchmeier, Alexander Bukreyev, Felicity Burt, Carmen Büttner, Charles H. Calisher, Mengji Cao, Inmaculada Casas, Kartik Chandran, Rémi N. Charrel, Krishna Kumar Chaturvedi, Kar Mun Chooi, Anya Crane, Elena Dal Bó, Juan Carlos de la Torre, William M. de Souza, Rik L. de Swart, Humberto Debat, Nolwenn M. Dheilly, Nicholas Di Paola, Francesco Di Serio, Ralf G. Dietzgen, Michele Digiaro, J. Felix Drexler, W. Paul Duprex, Ralf Dürrwald, Andrew J. Easton, Toufic Elbeaino, Koray Ergünay, Guozhong Feng, Andrew E. Firth, Anthony R. Fooks, Pierre B. H. Formenty, Juliana Freitas-Astúa, Selma Gago-Zachert, María Laura García, Adolfo García-Sastre, Aura R. Garrison, Thomas R. Gaskin, Wenjie Gong, Jean-Paul J. Gonzalez, JoëlleGoüy de Bellocq, Anthony Griffiths, Martin H. Groschup, Ines Günther, Stephan Günther, John Hammond, Yusuke Hasegawa, Kazusa Hayashi, Jussi Hepojoki, Colleen M. Higgins, Seiji Hongō, Masayuki Horie, Holly R. Hughes, Adam J. Hume, Timothy H. Hyndman, Kenichi Ikeda, Dàohóng Jiāng, Gilda B. Jonson, Sandra Junglen, Boris Klempa, Jonas Klingström, Hideki Kondō, Eugene V. Koonin, Mart Krupovic, Kenji Kubota, Gael Kurath, Lies Laenen, Amy J. Lambert, Jiànróng Lǐ, Jun-Min Li, Ran Liu, Igor S. Lukashevich, Robin M. MacDiarmid, Piet Maes, Marco Marklewitz, Sergio H. Marshall, Shin-Yi L. Marzano, John W. McCauley, Ali Mirazimi, Elke Mühlberger, Tomoyuki Nabeshima, Rayapati Naidu, Tomohide Natsuaki, Beatriz Navarro, José A. Navarro, Yutaro Neriya, Sergey V. Netesov, Gabriele Neumann, Norbert Nowotny, Márcio R. T. Nunes, Francisco M. Ochoa-Corona, Tomoyuki Okada, Gustavo Palacios, Vicente Pallás, Anna Papa, Sofia Paraskevopoulou, Colin R. Parrish, Alex Pauvolid-Corrêa, Janusz T. Pawęska, Daniel R. Pérez, Florian Pfaff, Richard K. Plemper, Thomas S. Postler, Lee O. Rabbidge, Sheli R. Radoshitzky, Pedro L. Ramos-González, Marius Rehanek, Renato O. Resende, Carina A. Reyes, Thaís C. S. Rodrigues, Víctor Romanowski, Dennis Rubbenstroth, Luisa Rubino, Jonathan A. Runstadler, Sead Sabanadzovic, Sabrina Sadiq, Maria S. Salvato, Takahide Sasaya, Martin Schwemmle, Stephen R. Sharpe, Mang Shi, Yoshifumi Shimomoto, Venkidusamy Kavi Sidharthan, Manuela Sironi, Sophie Smither, Jin-Won Song, Kirsten M. Spann, Jessica R. Spengler, Mark D. Stenglein, Ayato Takada, Sawana Takeyama, Akio Tatara, Robert B. Tesh, Natalie J. Thornburg, Xin Tian, Nicole D. Tischler, Yasuhiro Tomitaka, Keizō Tomonaga, Noël Tordo, Changchun Tu, Massimo Turina, Ioannis E. Tzanetakis, Anna Maria Vaira, Bernadette van den Hoogen, Bert Vanmechelen, Nikos Vasilakis, Martin Verbeek, Susanne von Bargen, Jiro Wada, Victoria Wahl, Peter J. Walker, Thomas B. Waltzek, Anna E. Whitfield, Yuri I. Wolf, Han Xia, Evanthia Xylogianni, Hironobu Yanagisawa, Kazutaka Yano, Gongyin Ye, Zhiming Yuan, F. Murilo Zerbini, Guilin Zhang, Song Zhang, Yong-Zhen Zhang, Lu Zhao, Arnfinn Lodden Økland
    In April 2023, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by one new family, 14 new genera, and 140 new species. Two genera and 538 species were renamed. One species was moved, and four were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.
    Microbiology Society, Aug. 2023, Journal of General Virology, 104(8) (8), English
    [Refereed]
    Scientific journal

  • Natsuki Kobayashi, Thach An Dang, Kieu Thi Minh Pham, Luis B Gómez Luciano, Ba Van Vu, Kosuke Izumitsu, Motoki Shimizu, Ken-ichi Ikeda, Wen-Hsiung Li, Hitoshi Nakayashiki
    Abstract Horizontal gene transfer (HGT) is a means of exchanging genetic material asexually. The process by which horizontally transferred genes are domesticated by the host genome is of great interest but is not well understood. In this study, we determined the telomere-to-telomere genome sequence of the wheat-infecting Pyricularia oryzae strain Br48. SNP analysis indicated that the Br48 strain is a hybrid of wheat- and Brachiaria-infecting strains by a sexual or parasexual cross. Comparative genomic analysis identified several megabase-scale “insertions” in the Br48 genome, some of which were possibly gained by HGT-related events from related species, such as P. pennisetigena or P. grisea. Notably, the mega-insertions often contained genes whose phylogeny is not congruent with the species phylogeny. Moreover, some of the genes have a close homolog even in distantly related organisms, such as basidiomycetes or prokaryotes, implying the involvement of multiple HGT events. Interestingly, the levels of the silent epigenetic marks H3K9me3 and H3K27me3 in a genomic region tended to be negatively correlated with the phylogenetic concordance of genes in the same region, suggesting that horizontally transferred DNA is preferentially targeted for epigenetic silencing. Indeed, the putative HGT-derived genes were activated when MoKmt6, the gene responsible for H3K27me3 modification, was deleted. Notably, these genes also tended to be up-regulated during infection, suggesting that they are now under host control and have contributed to establishing a fungal niche. In conclusion, this study suggests that epigenetic modifications have played an important role in the domestication of HGT-derived genes in the P. oryzae genome.
    Oxford University Press (OUP), Aug. 2023, Molecular Biology and Evolution, 40(9) (9)
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Dang Ngoc Minh, Yusaku Tsukahara, Dang An Thach, Ken-ich Ikeda, Hitoshi Nakayashiki
    Springer Science and Business Media LLC, Dec. 2022, Journal of General Plant Pathology, 89(2) (2), 77 - 83
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Ryutaro Morita, Naoko Crofts, Satoko Miura, Ken-ichi Ikeda, Naohiro Aoki, Hiroshi Fukayama, Naoko Fujita
    Abstract Rice is the model C3 crop for investigating the starch biosynthesis mechanism in endosperm because of its importance in grain production. However, little is known about starch biosynthesis in the vegetative organs of rice. In this study, we used novel rice mutants by inserting Tos17 into the starch synthase (SS) IIIb gene, which is mainly expressed in the leaf sheath (LS) and leaf blade (LB), and an ss1 mutant to clarify the differences in roles among SS isozymes during starch biosynthesis. Native polyacrylamide gel electrophoresis (PAGE)/activity staining for SS, using LS and LB of ss mutants, revealed that the lowest migrating SS activity bands on the gel were derived from SSIIIb activity and those of two ss3b mutants were not detected. The apparent amylose content of LS starch of ss3b mutants increased. Moreover, the chain-length distribution and size-exclusion chromatography analysis using ss mutants showed that SSIIIb and SSI synthesize the B2–B3 chain and A–B1 chain of amylopectin in the LS and LB respectively. Interestingly, we also found that starch contents were decreased in the LS and LB of ss3b mutants, although SSI deficiency did not affect the starch levels. All these results indicated that SSIIIb synthesizes the long chain of amylopectin in the LS and LB similar to SSIIIa in the endosperm, while SSI synthesizes the short chain in the vegetative organ as the same in the endosperm.
    Oxford University Press (OUP), Oct. 2022, Plant and Cell Physiology, 64(1) (1), 94 - 106, English, Domestic magazine
    [Refereed]
    Scientific journal

  • Fumihiro Nishimura, Haruka Fujisawa, Mitsutaka Mori, Chiharu Nakashima, Mitsuru Nakanishi, Yutaka Iwamoto, Genki Mimuro, Yoshihide Nakamura, Mayu Komori, Kenichi Ikeda
    Corresponding, Wiley, Oct. 2022, Plant Pathology, 71(8) (8), 1784 - 1792, English
    [Refereed]
    Scientific journal

  • Zikai Xiang, Daiki Okada, Soichiro Asuke, Hitoshi Nakayashiki, Kenichi Ikeda
    Corresponding, Wiley, Aug. 2022, Molecular Plant Pathology, 23(11) (11), 1658 - 1670, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Takano, T, Ikegaya, H, Ikeda, K, Nozaki, H, Sakayama, H
    Informa UK Limited, 2022, Phycologia, 61(4) (4), 425 - 435, English
    [Refereed]
    Scientific journal

  • Yoshifumi Shimomoto, Tomoyuki Okada, Kenichi Ikeda, Akio Tatara, Yusuke Hasegawa, Hironobu Yanagisawa, Sawana Takeyama, Kazusa Hayashi, Kazutaka Yano, Yasuaki Morita, Kenji Kubota
    Springer Science and Business Media LLC, Jan. 2022, Journal of General Plant Pathology, 88(1) (1), 69 - 80
    [Refereed]
    Scientific journal

  • First report of 'Candidatus Phytoplasma solani' associated with pepper chlorosis of sweet pepper, Capsicum annuum L., in Japan
    Yoshifumi Shimomoto,, Kenichi Ikeda, Yasushi Asahina,, Kazutaka Yano,, Misako Oka,, Tomoka Oki,, Junki Yamasaki,, Shigeharu Takeuchi,, Yasuaki Morita
    Jan. 2020, Journal of General Plant Pathology, 86(1) (1), 76 - 79, English
    [Refereed]
    Scientific journal

  • 森田 隆太郎, クロフツ 尚子, 柴谷 直樹, 三浦 聡子, 保坂 優子, 追留 那緒子, IKEDA KENICHI, 藤田 直子, FUKAYAMA HIROSHI
    CO2-responsive CCT protein (CRCT) is suggested to be a positive regulator of starch biosynthesis in the leaf sheaths of rice, regulating the expression levels of starch biosynthesis-related genes. In this study, the effects of CRCT expression levels on the expression of starch biosynthesis-related enzymes and the quality of starch were studied. Using native-PAGE/activity staining and immunoblotting, we found that the protein levels of starch synthase I, branching enzyme I, branching enzyme IIa, isoamylase 1 and phosphorylase 1 were largely correlated with the CRCT expression levels in the leaf sheaths of CRCT transgenic lines. In contrast, the CRCT expression levels largely did not affect the expression levels and/or activities of starch biosynthesis-related enzymes in the leaf blades and endosperm tissues. The analysis of the chain-length distribution of starch in the leaf sheaths showed that short chains with a degree of polymerization from 5 to 14 were increased in the overexpression lines but decreased in the knockdown lines. The amylose content of starch in the leaf sheath was greatly increased in the overexpression lines. In contrast, the molecular weight of the amylopectin of starch in the leaf sheath of overexpression lines did not change compared with those of the non-transgenic rice. These results suggest that CRCT can control the quality and the quantity of starch in the leaf sheath by regulating the expression of particular starch biosynthesis-related enzymes.
    May 2019, Plant and Cell Physiology, 60(5) (5), 961 - 972, English, Domestic magazine
    [Refereed]
    Scientific journal

  • Application of energy-filtering electron microscopy (EFEM) for analysis of hydrogen peroxide and lignin in epidermal walls of cucumber leaves triggered by acibenzolar-S-methyl treatment prior to inoculation with Colletotrichum orbiculare
    Park, P, Kurihara, T, Nita, M, IKEDA, K, Inoue, K, Ishii, H
    2019, Plant Pathology, 68, 1624 - 1634, English
    [Refereed]
    Scientific journal

  • Ohkita, S, Lee, Y, Nguyen, Q, Ikeda, K, Suzuki, N, Nakayashiki, H
    Three ourmia-like viruses, designated Pyricularia oryzae ourmia-like virus (PoOLV) 1 to 3, were identified in a wheat-infecting isolate of P. oryzae. The sizes of the full-length PoOLV1-3 genomes were determined to be 2,528, 1,671, and 2,557 nt. Interestingly, we also found two abundant single-stranded RNAs sharing their 5' terminal 25 and 255 nt with PoOLV1 RNA and PoOLV3 RNA, respectively. The PoOLV1- and PoOLV3-associated RNAs (ARNA1 and ARNA3) were 639 and 514 nt in length, and possessed one and two small ORFs, respectively. In the field isolates of P. oryzae, PoOLVs and ARNAs were detectable at varying levels, and the levels of PoOLV1 and ARNA1 as well as those of PoOLV3 and ARNA3, were tightly correlated. In addition, gene silencing of PoOLV1 and PoOLV3 resulted in a reduction of ARNA1 and ARNA3, respectively. There results indicated that replication of ARNA1 and ARNA3 was associated with PoOLV1 and PoOLV3, respectively.
    2019, Virology, 534, 25 - 35, English, International magazine
    [Refereed]
    Scientific journal

  • Yuka Mori, Yuki Hosoi, Shiho Ishikawa, Kazusa Hayashi, Yu Asai, Hideyuki Ohnishi, Mika Shimatani, Kanako Inoue, Kenichi Ikeda, Hitoshi Nakayashiki, Yasuyo Nishimura, Kouhei Ohnishi, Akinori Kiba, Kenji Kai, Yasufumi Hikichi
    © 2017 BSPP AND JOHN WILEY & SONS LTD After invasion into intercellular spaces of tomato plants, the soil-borne, plant-pathogenic Ralstonia solanacearum strain OE1-1 forms mushroom-shaped biofilms (mushroom-type biofilms, mBFs) on tomato cells, leading to its virulence. The strain OE1-1 produces aryl-furanone secondary metabolites, ralfuranones (A, B, J, K and L), dependent on the quorum sensing (QS) system, with methyl 3-hydroxymyristate (3-OH MAME) synthesized by PhcB as a QS signal. Ralfuranones are associated with the feedback loop of the QS system. A ralfuranone productivity-deficient mutant (ΔralA) exhibited significantly reduced growth in intercellular spaces compared with strain OE1-1, losing its virulence. To analyse the function of ralfuranones in mBF formation by OE1-1 cells, we observed cell aggregates of R. solanacearum strains statically incubated in tomato apoplast fluids on filters under a scanning electron microscope. The ΔralA strain formed significantly fewer microcolonies and mBFs than strain OE1-1. Supplementation of ralfuranones A, B, J and K, but not L, significantly enhanced the development of mBF formation by ΔralA. Furthermore, a phcB- and ralA-deleted mutant (ΔphcB/ralA) exhibited less formation of mBFs than OE1-1, although a QS-deficient, phcB-deleted mutant formed mBFs similar to OE1-1. Supplementation with 3-OH MAME significantly reduced the formation of mBFs by ΔphcB/ralA. The application of each ralfuranone significantly increased the formation of mBFs by ΔphcB/ralA supplied with 3-OH MAME. Together, our findings indicate that ralfuranones are implicated not only in the development of mBFs by strain OE1-1, but also in the suppression of QS-mediated negative regulation of mBF formation.
    Apr. 2018, Molecular Plant Pathology, 19(4) (4), 975 - 985, English
    [Refereed]
    Scientific journal

  • Quyet Nguyen, Akihide Iritani, Shuhei Ohkita, Ba V. Vu, Kana Yokoya, Ai Matsubara, Ken-Ichi Ikeda, Nobuhiro Suzuki, Hitoshi Nakayashiki
    NLM (Medline), Mar. 2018, Nucleic acids research, 46(5) (5), 2698, English
    Scientific journal

  • Quyet Nguyen, Akihide Iritani, Shuhei Ohkita, Ba Van Vu, Kana Yokoya, Ai Matsubara, Ken-ichi Ikeda, Nobuhiro Suzuki, NAKAYASHIKI HITOSHI
    Small RNA (sRNA)-mediated gene silencing phenomena, exemplified by RNA interference (RNAi), require a unique class of proteins called Argonautes (AGOs). An AGO protein typically forms a protein-sRNA complex that contributes to gene silencing using the loaded sRNA as a specificity determinant. Here, we show that MoAGO2, one of the three AGO genes in the fungus Pyricularia oryzae (Magnaporthe oryzae) interferes with RNAi. Gene knockout (KO) studies revealed that MoAGO1 and MoAGO3 additively or redundantly played roles in hairpin RNA- and retrotransposon (MAGGY)-triggered RNAi while, surprisingly, the KO mutants of MoAGO2 (Δmoago2) showed elevated levels of gene silencing. Consistently, transcript levels of MAGGY and mycoviruses were drastically reduced in Δmoago2, supporting the idea that MoAGO2 impeded RNAi against the parasitic elements. Deep sequencing analysis revealed that repeat- and mycovirus-derived small interfering RNAs were mainly associated with MoAGO2 and MoAGO3, and their populations were very similar based on their size distribution patterns and positional base preference. Site-directed mutagenesis studies indicated that sRNA binding but not slicer activity of MoAGO2 was essential for the ability to diminish the efficacy of RNAi. Overall, these results suggest a possible interplay between distinct sRNA-mediated gene regulation pathways through a competition for sRNA.
    Mar. 2018, Nucleic Acids Research, 46(5) (5), 2495 - 2508, English, International magazine
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Yutaka Iwamoto, Kai Inoue, Shinji Nishiguchi, Katsunari Matsuura, Masataka Aino, Hitoshi Nakayashiki, Kenichi Ikeda
    Lettuce big-vein disease, caused by Mirafiori lettuce big-vein virus and Lettuce big-vein associated virus, is suppressed when the pH of field soil becomes acidic. Therefore, we evaluated the effect of soil pH on the activities of Olpidium virulentus, the vector of the viruses. We found that acidic soil, pH less than 6.0, significantly reduced O. virulentus infection of the root and influenced the detection rate of zoospores released in the surrounding water. We concluded that acidic soil suppresses zoospore release from zoosporangia.
    Corresponding, SPRINGER JAPAN KK, Jul. 2017, JOURNAL OF GENERAL PLANT PATHOLOGY, 83(4) (4), 240 - 243, English
    [Refereed]
    Scientific journal

  • 岩本 豊, 井上 海, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 田中 正, 佐藤 博友, 西口 真嗣, 松浦 克成, 中野 伸一

    We conducted laboratory experiments to assess the disease control effects of pH-decreasing fertilizer as a new control material for lettuce big-vein disease. Results show that the occurrence of big-vein disease was suppressed in concomitance with the descent of soil pH by applying the fertilizer.

    The Kansai Plant Protection Society, May 2017, 関西病虫害研究会報, 59(59) (59), 51 - 53, Japanese
    [Refereed]
    Scientific journal

  • Tomoyuki Osato, Pyoyun Park, Kenichi Ikeda
    The Sclerotium is one of the most persistent organs in filamentous fungi. Control of sclerotial formation is promising in the prevention of sclerotial disease. In this study, cytological analyses of sclerotial development were conducted in Sclerotinia minor. Number and size of sclerotia were correlated with nutrient concentration of the media. Interruption of aeration by sealing with parafilm completely suppressed sclerotial formation. We also found that reactive oxygen species (ROS) generated two phases, i.e., hydrogen peroxide at sclerotial initial (SI) stage and O-2(-) at outer layer of sclerotial development/mature stages, during sclerotial formation. Ultrastructural analyses revealed that ROS was prominently produced at the outer layer of sclerotia in sclerotial mature (SM) phase. Although most of the inhibitors for ROS generation enzymes were ineffective for sclerotial formation, ascorbic acid, one of the scavengers of hydrogen peroxide, inhibited melanin biosynthesis during sclerotial maturation stage. The mycelia sealed with parafilm, when exogenously sprayed with hydrogen peroxide, could not produce sclerotium. These results indicated that ROS generation during sclerotial formation is mainly involved in the production of melanin layer. (C) 2016 British Mycological Society. Published by Elsevier Ltd. All rights reserved.
    Corresponding, ELSEVIER SCI LTD, Feb. 2017, FUNGAL BIOLOGY, 121(2) (2), 127 - 136, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Yoko Marutani, Yasuo Yamauchi, Mari Higashiyama, Akihito Miyoshi, Seiji Akimoto, Kanako Inoue, Ken-ichi Ikeda, Masaharu Mizutani, Yukihiro Sugimoto
    Light and temperature affect state transitions through changes in the plastoquinone (PQ) redox state in photosynthetic organisms. We demonstrated that light and/or heat treatment induced preferential photosystem (PS) I excitation by binding light-harvesting complex II (LHCII) proteins. The photosystem of wheat was in state 1 after dark overnight treatment, wherein PQ was oxidized and most of LHCII was not bound to PSI. At the onset of the light treatment [25 A degrees C in the light (100 A mu mol photons m(-2) s(-1))], two major LHCIIs, Lhcb1 and Lhcb2 were phosphorylated, and the PSI-LHCII supercomplex formed within 5 min, which coincided with an increase in the PQ oxidation rate. Heat treatment at 40 A degrees C of light-adapted wheat led to further LHCII protein phosphorylation of, resultant cyclic electron flow promotion, which was accompanied by ultrafast excitation of PSI and structural changes of thylakoid membranes, thereby protecting PSII from heat damage. These results suggest that LHCIIs are required for the functionality of wheat plant PSI, as it keeps PQ oxidized by regulating photochemical electron flow, thereby helping acclimation to environmental changes.
    SPRINGER, Jan. 2017, PHOTOSYNTHESIS RESEARCH, 131(1) (1), 41 - 50, English
    [Refereed]
    Scientific journal

  • Ryutaro Morita, Kanako Inoue, Ken-ichi Ikeda, Tomoko Hatanaka, Shuji Misoo, Hiroshi Fukayama
    CO2-responsive CCT protein (CRCT) is the suggested positive regulator of starch synthesis in vegetative organs, particularly the leaf sheath of rice. In this study, we analyzed the effects of the starch level in the leaf sheath on the photosynthetic rate in the leaf blade using CRCT overexpression and RNA interference (RNAi) knockdown transgenic rice grown under ambient (38 Pa) or elevated (100 Pa) CO2 conditions. In leaf sheath, the starch content was markedly changed in relation to CRCT expression levels under both CO2 conditions. In contrast, the soluble sugar and starch contents of the leaf blade were markedly increased in the knockdown line grown under elevated CO2 conditions. The overexpression or RNAi knockdown of CRCT did not cause large effects on the photosynthetic rate of the transgenic lines grown under ambient CO2 condition. However, the photosynthetic rate of the overexpression line was enhanced, while that of the knockdown line was substantially decreased under elevated CO2 conditions. These photosynthetic rates were weakly correlated with the nitrogen contents and negatively correlated with the total nonstructural carbohydrate contents. Thus, the capacity for starch synthesis in leaf sheath, which is controlled by CRCT, can indirectly affect the carbohydrate content, and then the photosynthetic rate in the leaf blade of rice grown under elevated CO2 conditions.
    OXFORD UNIV PRESS, Nov. 2016, PLANT AND CELL PHYSIOLOGY, 57(11) (11), 2334 - 2341, English
    [Refereed]
    Scientific journal

  • Yuka Mori, Kanako Inoue, Kenichi Ikeda, Hitoshi Nakayashiki, Chikaki Higashimoto, Kouhei Ohnishi, Akinori Kiba, Yasufumi Hikichi
    The mechanism of colonization of intercellular spaces by the soil-borne and vascular plant-pathogenic bacterium Ralstonia solanacearum strain OE1-1 after invasion into host plants remains unclear. To analyse the behaviour of OE1-1 cells in intercellular spaces, tomato leaves with the lower epidermis layers excised after infiltration with OE1-1 were observed under a scanning electron microscope. OE1-1 cells formed microcolonies on the surfaces of tomato cells adjacent to intercellular spaces, and then aggregated surrounded by an extracellular matrix, forming mature biofilm structures. Furthermore, OE1-1 cells produced mushroom-type biofilms when incubated in fluids of apoplasts including intercellular spaces, but not xylem fluids from tomato plants. This is the first report of biofilm formation by R. solanacearum on host plant cells after invasion into intercellular spaces and mushroom-type biofilms produced by R. solanacearum in vitro. Sugar application led to enhanced biofilm formation by OE1-1. Mutation of lecM encoding a lectin, RS-IIL, which reportedly exhibits affinity for these sugars, led to a significant decrease in biofilm formation. Colonization in intercellular spaces was significantly decreased in the lecM mutant, leading to a loss of virulence on tomato plants. Complementation of the lecM mutant with native lecM resulted in the recovery of mushroom-type biofilms and virulence on tomato plants. Together, our findings indicate that OE1-1 produces mature biofilms on the surfaces of tomato cells after invasion into intercellular spaces. RS-IIL may contribute to biofilm formation by OE1-1, which is required for OE1-1 virulence.
    WILEY-BLACKWELL, Aug. 2016, MOLECULAR PLANT PATHOLOGY, 17(6) (6), 890 - 902, English
    [Refereed]
    Scientific journal

  • Daisuke Takagi, Kentaro Ifuku, Ken-ichi Ikeda, Kanako Ikeda Inoue, Pyoyun Park, Masahiro Tamoi, Hironori Inoue, Katsuhiko Sakamoto, Ryota Saito, Chikahiro Miyake
    Lipid-derived reactive carbonyl species (RCS) possess electrophilic moieties and cause oxidative stress by reacting with cellular components. Arabidopsis (Arabidopsis thaliana) has a chloroplast-localized alkenal/one oxidoreductase (AtAOR) for the detoxification of lipid-derived RCS, especially alpha,beta-unsaturated carbonyls. In this study, we aimed to evaluate the physiological importance of AtAOR and analyzed AtAOR (aor) mutants, including a transfer DNA knockout, aor (T-DNA), and RNA interference knockdown, aor (RNAi), lines. We found that both aor mutants showed smaller plant sizes than wild-type plants when they were grown under day/night cycle conditions. To elucidate the cause of the aor mutant phenotype, we analyzed the photosynthetic rate and the respiration rate by gas-exchange analysis. Subsequently, we found that both wildtype and aor (RNAi) plants showed similar CO2 assimilation rates; however, the respiration rate was lower in aor (RNAi) than in wild-type plants. Furthermore, we revealed that phosphoenolpyruvate carboxylase activity decreased and starch degradation during the night was suppressed in aor (RNAi). In contrast, the phenotype of aor (RNAi) was rescued when aor (RNAi) plants were grown under constant light conditions. These results indicate that the smaller plant sizes observed in aor mutants grown under day/night cycle conditions were attributable to the decrease in carbon utilization during the night. Here, we propose that the detoxification of lipid-derived RCS by AtAOR in chloroplasts contributes to the protection of dark respiration and supports plant growth during the night.
    AMER SOC PLANT BIOLOGISTS, Apr. 2016, PLANT PHYSIOLOGY, 170(4) (4), 2024 - 2039, English
    [Refereed]
    Scientific journal

  • 走査型電子顕微鏡を用いた代替電子染色剤の選抜法
    池田 健一, 嘉久 大毅, 井上 加奈子, 朴 杓允
    (一社)医学生物学電子顕微鏡技術学会, Mar. 2016, 医学生物学電子顕微鏡技術学会誌, 29(2) (2), 81 - 81, Japanese
    [Refereed]

  • 糸状菌病害の組織特異的感染戦略 －いもち病菌の生い立ちを紐解く－
    IKEDA KENICHI, 井上 加奈子, 森田 雄一, 伊藤 里紗, 中本 知里, 植 宏美, 翠川 陽大, 藤原 有紗, Talbot J Nicholas, NAKAYASHIKI HITOSHI
    日本植物病理学会, 2016, 植物感染生理談話会論文集, (51) (51), 83 - 94, Japanese
    [Invited]
    Symposium

  • Kanako Inoue, Harumasa Kitaoka, Pyoyun Park, Kenichi Ikeda
    The adhesive ability of spores is important for fungal pathogens to manifest pathogenicity. Hydrophobins found on the outer surface of conidia and hyphae may be involved in adhesion or communication between the fungus and its environment. The effects of two hydrophobins, class I Mpg1 and class II Mhp1, on adhesion, germling differentiation, and pathogenicity of a wheat isolate of Magnaporthe oryzae were evaluated. We conducted gene knockdown or knockout experiments to obtain hydrophobin mutants. We found several discrepancies from previous studies. The Mpg1-silenced mutants had various levels of Mpg1 transcription, and Mpg1 expression was correlated with adhesion, germling differentiation, and pathogenicity. We also obtained mhp1 null mutants that had full pathogenicity on wheat and barley leaves. These results suggest that Mpg1 is directly contributing to adhesion, but Mhp1 is not essential for the infection process in the wheat isolate of M. oryzae.
    Corresponding, SPRINGER JAPAN KK, Jan. 2016, JOURNAL OF GENERAL PLANT PATHOLOGY, 82(1) (1), 18 - 28, English
    [Refereed]
    Scientific journal

  • Ryo Shukunami, Yutaka Iwamoto, Shinji Sugiura, Kentaro Ikeda, Hitohsi Nakayashiki, Kenichi Ikeda
    We designed specific primers for Coniothyrium minitans, a sclerotia-parasitizing biocontrol agent (BCA), and quantified its biomass in the field by quantitative polymerase chain reaction. Because chemical properties of the soil can decrease the DNA extraction efficiency and DNA polymerase activity, standard curves were calibrated by adding known concentrations of BCA to sterilized field soil. The BCA biomass was monitored over time and after various treatments. Because the distribution of the BCA biomass was uneven in the field, a method to spread BCA uniformly in the field is needed.
    Corresponding, SPRINGER JAPAN KK, Jan. 2016, JOURNAL OF GENERAL PLANT PATHOLOGY, 82(1) (1), 51 - 56, English
    [Refereed]
    Scientific journal

  • Takahiro Uwamori, Kanako Inoue, Chiaki Kida, Yuichi Morita, Pyoyun Park, Hitoshi Nakayashiki, Satoko Kanematsu, Kenichi Ikeda
    Hyphal interactions of 10 Rosellinia necatrix isolates were analyzed. Most incompatible pairings produced broad melanized barrage lines, with a few exceptions. The variation in barrage lines revealed using light microscopy might result from distinct frequencies of different hyphal interaction types. In incompatible pairings, hyphal anastomosis was rarely observed. Activated-charcoal treatment of oatmeal agar media prevented both incompatible and compatible reactions. FUN-1 staining showed vacuole dysfunction at hyphal interaction sites, and the localization of a Lifeact-GFP mutant revealed that actin dynamics was prominent in compatible pairings. These results suggested that self/nonself recognition commonly proceeded to vacuole dysfunction and that actin dynamics played a critical role in the restoration of vacuole integrity.
    Corresponding, SPRINGER JAPAN KK, Nov. 2015, JOURNAL OF GENERAL PLANT PATHOLOGY, 81(6) (6), 420 - 428, English
    [Refereed]
    Scientific journal

  • Hiroki Kaku, Kanako Inoue, Yoshinori Muranaka, Pyoyun Park, Kenichi Ikeda
    Uranyl salts are toxic and radioactive; therefore, several studies have been conducted to screen for substitutes of electron stains. In this regard, the contrast evaluation process is time consuming and the results obtained are inconsistent. In this study, we developed a novel contrast evaluation method using affinity beads and a backscattered electron image (BSEI), obtained using scanning electron microscopy. The contrast ratios of BSEI in each electron stain treatment were correlated with those of transmission electron microscopic images. The affinity beads bound to cell components independently. Protein and DNA samples were enhanced by image contrast treated with electron stains; however, this was not observed for sugars. Protein-conjugated beads showed an additive effect of image contrast when double-stained with lead. However, additive effect of double staining was not observed in DNA-conjugated beads. The varying chemical properties of oligopeptides showed differences in image contrast when treated with each electron stain. This BSEI-based evaluation method not only enables screening for alternate electron stains, but also helps analyze the underlying mechanisms of electron staining of cellular structures.
    Corresponding, OXFORD UNIV PRESS, Oct. 2015, MICROSCOPY, 64(5) (5), 361 - 368, English
    [Refereed]
    Scientific journal

  • Mechanisms of biofilm formation by Ralstonia solanacearum and its involvement in the bacterial virulence
    曳地 康史, 森 友花, 石川 詩歩, 東本 周樹, 井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 木場 章範, 大西 浩平, 甲斐 建次
    Phytopathological Society of Japan, Sep. 2015, PSJ Plant-Microbe Interactions Symposium Report, (50) (50), 11 - 21, Japanese
    [Invited]
    Symposium

  • Epigenetic gene regulation in the rice blast fungus Pyricularia oryzae
    Pham Thi Minh Kieu, 井上 義博, Vu Van Ba, IKEDA KENICHI, NAKAYASHIKI HITOSHI
    Phytopathological Society of Japan, Sep. 2015, PSJ Plant-Microbe Interactions Symposium Report, (50) (50), 113 - 121, Japanese
    [Invited]
    Symposium

  • K. Takasuka, T. Yasui, T. Ishigami, K. Nakata, R. Matsumoto, K. Ikeda, K. Maeto
    The Company of Biologists, Aug. 2015, Journal of Experimental Biology, 218(15) (15), 2326 - 2332, English
    [Refereed]
    Scientific journal

  • Kieu Thi Minh Pham, Yoshihiro Inoue, Ba Van Vu, Hanh Hieu Nguyen, Toru Nakayashiki, Ken-ichi Ikeda, Hitoshi Nakayashiki
    Here we report the genetic analyses of histone lysine methyltransferase (KMT) genes in the phytopathogenic fungus Magnaporthe oryzae. Eight putative M. oryzae KMT genes were targeted for gene disruption by homologous recombination. Phenotypic assays revealed that the eight KMTs were involved in various infection processes at varying degrees. Moset1 disruptants (Delta moset1) impaired in histone H3 lysine 4 methylation (H3K4me) showed the most severe defects in infection-related morphogenesis, including conidiation and appressorium formation. Consequently,Delta moset1 lost pathogenicity on wheat host plants, thus indicating that H3K4me is an important epigenetic mark for infection-related gene expression in M. oryzae. Interestingly, appressorium formation was greatly restored in the Delta moset1 mutants by exogenous addition of cAMP or of the cutin monomer, 16-hydroxypalmitic acid. The Delta moset1 mutants were still infectious on the super-susceptible barley cultivar Nigrate. These results suggested that MoSET1 plays roles in various aspects of infection, including signal perception and overcoming host-specific resistance. However, since Delta moset1 was also impaired in vegetative growth, the impact of MoSET1 on gene regulation was not infection specific. ChIP-seq analysis of H3K4 di- and tri-methylation (H3K4me2/me3) and MoSET1 protein during infection-related morphogenesis, together with RNA-seq analysis of the Delta moset1 mutant, led to the following conclusions: 1) Approximately 5% of M. oryzae genes showed significant changes in H3K4-me2 or -me3 abundance during infection-related morphogenesis. 2) In general, H3K4-me2 and -me3 abundance was positively associated with active transcription. 3) Lack of MoSET1 methyltransferase, however, resulted in up-regulation of a significant portion of the M. oryzae genes in the vegetative mycelia (1,491 genes), and during infection-related morphogenesis (1,385 genes), indicating that MoSET1 has a role in gene repression either directly or more likely indirectly. 4) Among the 4,077 differentially expressed genes (DEGs) between mycelia and germination tubes, 1,201 and 882 genes were up- and down-regulated, respectively, in a Moset1-dependent manner. 5) The Moset1-dependent DEGs were enriched in several gene categories such as signal transduction, transport, RNA processing, and translation.
    PUBLIC LIBRARY SCIENCE, Jul. 2015, PLOS GENETICS, 11(7) (7), e1005385, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Inhibition of abscission layer formation by an interaction of two seed-shattering loci, sh4 and qSH3, in rice
    Chizuru Inoue, Than Myint Htun, Kanako Inoue, Ken-ichi Ikeda, Takashige Ishii, Ryo Ishikawa
    Loss of seed shattering was one of the key phenotypic changes selected for in the domestication of many crop species. Asian cultivated rice, Oryza sativa L., was domesticated from its wild ancestor, 0. rufipogon, and three seed-shattering loci, qSH1, sh4 and qSH3, have been reported to be involved in the loss of seed shattering in cultivated rice. Here, we analysed the seed-shattering behaviour of wild rice using introgression lines carrying the cultivated alleles from 0. sativa Nipponbare in the genetic background of wild rice, 0. rufipogon W630. We first carried out fine mapping of the qSH3 region and found that the qSH3 locus is localized in an 850-kb region on chromosome 3. We then analysed the effects of the Nipponbare alleles at sh4 and qSH3 on seed-shattering behaviour in wild rice, as a mutation at qSH1 was not commonly found in rice cultivars. Seed-shattering behaviour did not change in the two types of introgression line independently carrying the Nipponbare-homozygous alleles at sh4 or qSH3 in the genetic background of wild rice. However, the introgression lines having the Nipponbare-homozygous alleles at both sh4 and qSH3 showed a reduction in the degree of seed shattering. Histological and scanning electron microscopy analyses revealed that abscission layer formation was inhibited around the vascular bundles in these lines. Since the qSH3 region, as well as sh4, has been shown to be under artificial selection, the interaction of mutations at these two loci may have played a role in the initial loss of seed shattering during rice domestication.
    GENETICS SOC JAPAN, Feb. 2015, GENES & GENETIC SYSTEMS, 90(1) (1), 1 - 9, English
    [Refereed]
    Scientific journal

  • Polycationic Ruthenium Red is Reactive with the Extracellular Matrix Produced by Plant Pathogenic Fungi
    Ueno S, Inoue K, Ishii H, Jiang S, Adachi Y, Kanematsu S, Park Pyoyun, IKEDA KENICHI
    医学生物学電子顕微鏡技術学会, 2015, Journal of Electron Microscopy Technology for Medicine and Biology, 28(2) (2), 1 - 7, English
    [Refereed]
    Scientific journal

  • Yoko Marutani, Yasuo Yamauchi, Akihito Miyoshi, Kanako Inoue, Ken-ichi Ikeda, Masaharu Mizutani, Yukihiro Sugimoto
    Photosystems of higher plants alleviate heat-induced damage in the presence of light under moderate stressed conditions; however, in the absence of light (i.e., in the dark), the same plants are damaged more easily. (Yamauchi and Kimura, 2011) We demonstrate that regulating photochemical energy transfer in heat-treated wheat at 40 degrees C with light contributed to heat tolerance of the photosystem. Chlorophyll fluorescence analysis using heat-stressed wheat seedlings in light showed increased non-photochemical quenching (NPQ) of chlorophyll fluorescence, which was due to thermal dissipation that was increased by state 1 to state 2 transition. Transmission electron microscopy revealed structural changes in thylakoid membranes, including unstacking of grana regions under heat stress in light. It was accompanied by the phosphorylation of thylakoid proteins such as D1 and D2 proteins and the light harvesting complex II proteins Lhcb1 and Lhcb2. These results suggest that heat stress at 40 degrees C in light induces state 1 to state 2 transition for the preferential excitation of photosystem I (PSI) by phosphorylating thylakoid proteins more strongly. Structural changes of thylakoid membrane also assist the remodeling of photosystems and regulation of energy distribution by transition toward state 2 probably contributes to plastoquione oxidation; thus, light-driven electrons flowing through PSI play a protective role against PSII damage under heat stress.
    MDPI AG, Dec. 2014, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 15(12) (12), 23042 - 23058, English
    [Refereed]
    Scientific journal

  • Hiroko Kitagawa, Saki Shimoi, Kanako Inoue, Pyoyun Park, Kenichi Ikeda
    We had previously obtained collagenolytic/gelatinolytic bacteria, which degrade the fungal extracellular matrix, to establish a novel biological control measure that inhibits germling adhesion of airborne phytopathogenic fungi on the host plant surface. By using barley-Magnaporthe oryzae pathosystem, Chryseobacterium sp. was most effective biocontrol agents as tested. The selected bacteria were evaluated for durable disease protection against M. oryzae on barley leaves by using chloramphenicol-resistant mutants. Chryseobacterium sp. from the soil was less likely to settle on leaf surfaces. Therefore, we tried to manipulate Chryseobacterium sp. to inhabit the leaf's surface. The gelatin supplementation dramatically improved the settlement of gelatinolytic bacteria Chryseobacterium sp. from the soil, and the disease protection effect lasted for more than 2 weeks on barley. Moreover, exploitation of Chryseobacterium sp. for disease protection was extended against other airborne pathogens, Alternaria alternata Japanese pear pathotype on Japanese pear and Colletotrichum orbiculare on cucumber. (C) 2014 Elsevier Inc. All rights reserved.
    Corresponding, ACADEMIC PRESS INC ELSEVIER SCIENCE, Apr. 2014, BIOLOGICAL CONTROL, 71, 1 - 6, English
    [Refereed]
    Scientific journal

  • Ralstonia solanacearumのlecMは細胞間隙への固着に関与する(The lecM of Ralstonia solanacearum is involved in its attachment to the intercellular spaces)
    森 友花, 井上 加奈子, 池田 健一, 木場 章範, 大西 浩平, 曵地 康史
    日本細菌学会, Feb. 2014, 日本細菌学雑誌, 69(1) (1), 213 - 213, English
    [Refereed]

  • 上森 喬大, 井上 加奈子, 北澤 大知, 木田 千晶, 中屋敷 均, 兼松 聡子, 池田 健一
    日本土壌微生物学会, 2014, 土と微生物, 68(1) (1), 50 - 50, Japanese
    [Refereed]

  • 齊藤 亮太, 伊福 健太郎, 池田 健一, 井上 加奈子, 三宅 親弘
    一般社団法人 日本土壌肥料学会, 2014, 日本土壌肥料学会講演要旨集, 60(0) (0), 288 - 288, Japanese
    [Refereed]

  • Ken-ichi Ikeda, Ba Van Vu, Naoki Kadotani, Masaki Tanaka, Toshiki Murata, Kohta Shiina, Izumi Chuma, Yukio Tosa, Hitoshi Nakayashiki
    The long terminal repeat retrotransposon, Magnaporthe gypsy-like element (MAGGY), has been shown to be targeted for cytosine methylation in a subset of Magnaporthe oryzae field isolates. Analysis of the F-1 progeny from a genetic cross between methylation-proficient (Br48) and methylation-deficient (GFSI1-7-2) isolates revealed that methylation of the MAGGY element was governed by a single dominant gene. Positional cloning followed by gene disruption and complementation experiments revealed that the responsible gene was the DNA methyltransferase, MoDMT1, an ortholog of Neurospora crassa Dim-2. A survey of MAGGY methylation in 60 Magnaporthe field isolates revealed that 42 isolates from rice, common millet, wheat, finger millet, and buffelgrass were methylation proficient while 18 isolates from foxtail millet, green bristlegrass, Japanese panicgrass, torpedo grass, Guinea grass, and crabgrass were methylation deficient. Phenotypic analyses showed that MoDMT1 plays no major role in development and pathogenicity of the fungus. Quantitative polymerase chain reaction analysis showed that the average copy number of genomic MAGGY elements was not significantly different between methylation-deficient and -proficient field isolates even though the levels of MAGGY transcript were generally higher in the former group. MoDMT1 gene sequences in the methylation-deficient isolates suggested that at least three independent mutations were responsible for the loss of MoDMT1 function. Overall, our data suggest that MoDMT1 is not essential for the natural life cycle of the fungus and raise the possibility that the genus Magnaporthe may be losing the mechanism of DNA methylation on the evolutionary time scale.
    GENETICS SOC AM, Nov. 2013, GENETICS, 195(3) (3), 845 - 855, English
    [Refereed]
    Scientific journal

  • Kenichi Ikeda, Kanako Inoue, Chiaki Kida, Takahiro Uwamori, Atsuko Sasaki, Satoko Kanematsu, Pyoyun Park
    Heterogenic incompatibility is considered a defense mechanism against deleterious intruders such as mycovirus. Rosellinia necatrix shows strong heterogenic incompatibility. In the heterogenic incompatibility reaction, the approaching hyphae hardly anastomosed, a distinctive barrage line formed, and green fluorescent protein (GFP)-labeled hyphae quickly lost their fluorescence when encountering incompatible hyphae. In this study, transmission of a hypovirulence-conferring mycovirus to strains with different genetic backgrounds was attempted. Various chemical reagents considered to affect the programmed cell death pathway or cell wall modification were examined. Treatment with zinc compounds was shown to aid in transmission of mycoviruses to strains with different genetic backgrounds. In incompatible pairings, treatment with zinc compounds accelerated hyphal anastomosis; moreover, cytosolic GFP was transmitted to the newly joined hyphae. These results suggest that zinc compounds not only increase hyphal anastomosis but also attenuate heterogenic incompatibility.
    AMER SOC MICROBIOLOGY, Jun. 2013, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79(12) (12), 3684 - 3691, English
    [Refereed]
    Scientific journal

  • Yuichi Morita, Gang-Su Hyon, Naoki Hosogi, Nao Miyata, Hitoshi Nakayashiki, Yoshinori Muranaka, Noriko Inada, Pyoyun Park, Kenichi Ikeda
    Black spot disease, Alternaria alternataJapanese pear pathotype, produces the host-specific toxin AK-toxin, an important pathogenicity factor. Previously, we have found that hydrogen peroxide is produced in the hyphal cell wall at the plantpathogen interaction site, suggesting that the fungal reactive oxygen species (ROS) generation machinery is important for pathogenicity. In this study, we identified two NADPH oxidase (NoxA and NoxB) genes and produced nox disruption mutants. noxA and noxB disruption mutants showed increased hyphal branching and spore production per unit area. Surprisingly, only the noxB disruption mutant compromised disease symptoms. A fluorescent protein reporter assay revealed that only NoxB localized at the appressoria during pear leaf infection. In contrast, both NoxA and NoxB were highly expressed on the cellulose membrane, and these Nox proteins were also localized at the appressoria. In the noxB disruption mutant, we could not detect any necrotic lesions caused by AK-toxin. Moreover, the noxB disruption mutant did not induce papilla formation on pear leaves. Ultrastructural analysis revealed that the noxB disruption mutant also did not penetrate the cuticle layer. Moreover, ROS generation was not essential for penetration, suggesting that NoxB may have an unknown function in penetration. Taken together, our results suggest that NoxB is essential for aggressiveness and basal pathogenicity in A.alternata.
    WILEY-BLACKWELL, May 2013, MOLECULAR PLANT PATHOLOGY, 14(4) (4), 365 - 378, English
    [Refereed]
    Scientific journal

  • マイコウイルス伝搬の障壁となる細胞質不和合性機構とその克服
    IKEDA KENICHI
    2013, JATAFFジャーナル, 1, 9 - 13, Japanese
    [Invited]
    Scientific journal

  • 塩化ハフニウム
    堀内 喜高, 池田 健一, 井上 加奈子, 兼松 聡子, 朴 杓允
    (一社)医学生物学電子顕微鏡技術学会, Apr. 2012, 医学生物学電子顕微鏡技術学会誌, 26(1) (1), 35 - 36, Japanese
    [Refereed]

  • 北川 博子, 井上 加奈子, 下井 沙紀, 池田 健一, 朴 杓允
    日本土壌微生物学会, 2012, 土と微生物, 66(2) (2), 89 - 89, Japanese
    [Refereed]

  • 木田 千晶, 井上 加奈子, 朴 杓允, 池田 健一
    日本土壌微生物学会, 2012, 土と微生物, 66(2) (2), 89 - 89, Japanese
    [Refereed]

  • Ikeda Kenichi, Inoue Kanako, Kida Chiaki, Uwamori Takahiro, Kanematsu Satoko, Park Pyoyun
    生物防除を成功させるためには,病原菌とそれを取り巻く微生物群の生態的特徴を理解する必要がある。果樹類紋羽病菌は菌糸を伸展させて伝搬するタイプの病原菌であり,生物防除を利用するのに適している。さらに,土壌中の微生物分の影響を除去するために,病原菌細胞内で増殖・伝搬できる菌類ウイルスの利用(ヴァイロコントロール)が効果的であると考えられる。これまでに,白紋羽病菌を対象として,病原力低下作用を有する菌類ウイルスを明らかとし,それらを任意菌株へ伝搬させる手法の確立を進めている。
    Japanese Society of Soil Microbiology, 2012, Soil Microorganisms, 66(2) (2), 55 - 57, Japanese
    Symposium

  • IKEDA KENICHI
    In the process of disease protection, we encounter numerous problems such as breakdown of disease resistance cultivar and emergence of fungicide tolerant pathogen. For the success of durable disease protection, we have to study the infection strategies and lifecycles of pathogen, and prepare multiple disease protection measures. Most of the disease resistance (R) genes function as a receptor for pathogen. However, most of the R genes sense dispensable factor for life or pathogenicity. Once the dispensable factors mutate, the disease resistance cultivars carrying R genes fail to sense pathogen, causing the breakdown of disease resistance. If an R gene senses essential factor for life or pathogenicity, that is Achilles' heel of the pathogen, it would be a durable R gene. I present a possibility to create novel R gene by directed evolution.
    The Society of Crop Science and Breeding in Kinki, Japan, 2012, Journal of Crop Research, 57, 61 - 66, Japanese
    Symposium

  • Kanako Inoue, Tomohiro Tsurumi, Hideo Ishii, Pyoyun Park, Kenichi Ikeda
    Azoxystrobin (AZ), a strobilurin-derived fungicide, is known to inhibit mitochondrial respiration in fungi by blocking the electron transport chain in the inner mitochondrial membrane. Germination was strongly inhibited when Botrytis cinerea spore suspension was treated with AZ and the alternative oxidase (AOX) inhibitors, salicylhydroxamic acid (SHAM) and n-propyl gallate. However, chemical death indicators trypan blue and propidium iodide showed that those spores were still alive. When the spore suspension in the AZ and SHAM solution was replaced with distilled water, the germination rate almost recovered, at least during the first 2 days of incubation with AZ and SHAM solution. No morphological alteration was detected in the cells treated with AZ and SHAM, especially in mitochondria, using transmission electron microscopy. Therefore, simultaneous application of AZ and AOX inhibitors has a fungistatic, rather than a fungicidal, action.
    OXFORD UNIV PRESS, Jan. 2012, FEMS MICROBIOLOGY LETTERS, 326(1) (1), 83 - 90, English
    [Refereed]
    Scientific journal

  • Comparative study of ultrathin section shrinkage in various epoxy resin blocks: sectioning from Spurr's block causes shrinkage in the longitudinal direction
    Inoue K, Morita Y, Kanematsu S, Park P, IKEDA KENICHI
    医学生物学電子顕微鏡技術学会, 2012, Journal of Electron Microscopy Technology for Medicine and Biology, 26(1) (1), 8 - 11, English
    [Refereed]
    Scientific journal

  • Kanako Inoue, Takatoshi Onoe, Pyoyun Park, Kenichi Ikeda
    Magnaporthe oryzae germlings tightly attach to the host surface by producing extracellular matrix (ECM) from germ tubes and appressoria, which are important for the early infection process. To understand the adhesion mechanisms of ECM during differentiation of infection structure, we evaluated the effects of various enzymes on M. oryzae germlings and the disease symptoms of the host plant, wheat. Treatment with beta-mannosidase, collagenase N-2, collagenase S-1, or gelatinase B at 1-h postinoculation (hpi) resulted in germling detachment, although producing normal appressoria. Treatment with matrix metalloproteinases (MMPs) at 6 hpi also caused germling detachment. Furthermore, we confirmed by the inoculation tests and scanning electron microscopy that the germlings on the wheat plant were removed and did not manifest pathogenicity on treatment with MMPs. The most effective MMPs were crude collagenase, collagenase S-1, and gelatinase B, suggesting that the application of MMPs is promising for crop protection from fungal diseases by its detachment action.
    OXFORD UNIV PRESS, Oct. 2011, FEMS MICROBIOLOGY LETTERS, 323(1) (1), 13 - 19, English
    [Refereed]
    Scientific journal

  • 塩化サマリウムによるウラン代替染色法
    池田 健一, 井上 加奈子, 兼松 聡子, 朴 杓允
    (一社)医学生物学電子顕微鏡技術学会, Sep. 2011, 医学生物学電子顕微鏡技術学会誌, 25(2) (2), 52 - 52, Japanese
    [Refereed]

  • Ken-Ichi Ikeda, Kanako Inoue, Satoko Kanematsu, Yoshitaka Horiuchi, Pyoyun Park
    This ultrastructural study showed that nonisotopic methanolic hafnium chloride and aqueous lead solution was an excellent new electron stain for enhancing TEM contrasts of fungal and plant cell structures. The ultrastructural definition provided by the new stain was often superior to that provided by conventional staining with uranyl acetate and lead. Definition of fine ultrastructure was also supported by quantitative data on TEM contrast ratios of organelles and components in fungal and plant cells. In particular, polysaccharides, which were localized in cell walls, glycogen particles, starch grains, and plant Golgi vesicle components, were much more reactive to the new stain than to the conventional one. The new nonisotopic stain is useful for enhancing the contrast of ultrastructure in biological tissues and is a safer alternative to uranyl acetate. Microsc. Res. Tech. 74: 825-830, 2011. (C) 2010 Wiley-Liss, Inc.
    WILEY, Sep. 2011, MICROSCOPY RESEARCH AND TECHNIQUE, 74(9) (9), 825 - 830, English
    [Refereed]
    Scientific journal

  • Kanako Inoue, Satoko Kanematsu, Pyoyun Park, Kenichi Ikeda
    When the mycelia of Helicobasidium mompa encounter mycelia with a different genetic background, distinct demarcation lines form. The hyphae of H. mompa induce heterogenic incompatibility accompanied by active programmed cell death (PCD) process. In this study, we observed hyphal interaction between compatible and incompatible H. mompa pairs by means of light and electron microscopy. PCD started with one of the two approaching hyphae. Heterochromatin condensation and genomic DNA laddering were not observed. Moreover, cell damage began with the tonoplast and continued with the plasma membrane and nuclear membrane, suggesting that the PCD observed in heterogenic incompatibility of H. mompa is a vacuole-mediated process.
    OXFORD UNIV PRESS, Feb. 2011, FEMS MICROBIOLOGY LETTERS, 315(2) (2), 94 - 100, English
    [Refereed]
    Scientific journal

  • Samarium chloride electron staining substitute for uranyl acetate staining
    IKEDA KENICHI, Inoue K, Kanematsu S, Park P
    2011, Journal of Electron Microscopy Technology for Medicine and Biology, 25, 106, Japanese
    Symposium

  • Reducing effect on peeling of speciment from resin by treatment with periodic acid
    Park P, Miyahara F, Shimoi S, Inoue K, IKEDA KENICHI
    2011, Journal of Electron Microscopy Technology for Medicine and Biology, 25, 116, Japanese
    Symposium

  • Kenichi Ikeda, Kanako Inoue, Hitoshi Nakamura, Taiki Hamanaka, Tatsuro Ohta, Hirotomo Kitazawa, Chiaki Kida, Satoko Kanematsu, Pyoyun Park
    When mycelia of Rosellinia necatrix encounter mycelia of a different genetic strain, distinct barrage lines are formed between the two. These barrages have variable features such as pigmented pseudosclerotia structures, a clear zone, fuzzy hair-like mycelia, or tuft-like mycelia, suggesting that mycelial incompatibility triggers a number of cellular reactions. In this study, to evaluate cellular reactions we performed genetic analysis of mycelial incompatibility of R. nectarix, using 20 single ascospore isolates from single perithecia. Mycelial interaction zones were removed by spatula and cellular reactions studied on oatmeal agar media. The interaction zones were categorized into types such as sharp or wide lines, with or without melanin, and combinations of these. Although various reaction types were observed, we were able to identify a single genetic factor that appears to be responsible for the barrage line formation within oatmeal agar medium. DNA polymorphism analysis identified parental isolates and revealed that R. necatrix has a heterothallic life cycle. (C) 2010 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
    ELSEVIER SCI LTD, Jan. 2011, FUNGAL BIOLOGY, 115(1) (1), 80 - 86, English
    [Refereed]
    Scientific journal

  • Kanako Inoue, Satoko Kanematsu, Pyoyun Park, Kenichi Ikeda
    When the mycelia of Rosellinia necatrix encounter mycelia with a different genetic background, distinct barrage lines form. In this study, we observed hyphal interactions between compatible and incompatible R. necatrix pairs by means of light and electron microscopy. Although we observed perfect hyphal anastomosis in compatible pairs of isolates, the hyphae never anastomosed in incompatible pairs (i.e., the hyphae remained parallel or crossed over without merging). These behaviours appeared to result from the detection of or failure to detect one or more diffusible factors. The attraction to other hyphae in pairs of incompatible isolates was increased by supplementation of the growing medium with activated charcoal, although no anastomosis was observed and ultrastructural observation confirmed a lack of hyphal anastomosis. Programmed cell death (PCD) started with one of the two approaching hyphae. Heterochromatin condensation and genomic DNA fragmentation were not observed. Moreover, cell damage began with the tonoplast and continued with the plasma and nuclear membranes, suggesting that the PCD observed in heterogenic incompatibility of R. necatrix was a vacuole-mediated process. (C) 2010 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
    ELSEVIER SCI LTD, Jan. 2011, FUNGAL BIOLOGY, 115(1) (1), 87 - 95, English
    [Refereed]
    Scientific journal

  • Comparative analysis of vacuole-mediated programmed cell death between heterogenic incompatibility both in Rosellinia necatrix and Helicobasidium mompa and authophagy in Magnaporthe oryzae
    Inoue K, Kanematsu S, Park P, IKEDA KENICHI
    2011, Journal of Electron Microscopy Technology for Medicine and Biology, 25, 113, Japanese
    Symposium

  • Characterization of ROS production at plant-microbe interaction sites in Alternaria alternata Japanese pear pathotype
    Morita Y, Hyon GS, IKEDA KENICHI, Park P
    2011, Journal of Electron Microscopy Technology for Medicine and Biology, 25, 115, Japanese
    Symposium

  • Rosellinia Necarixの細胞質不和合性反応における細胞像の経時的解析
    井上 加奈子, 池田 健一, 兼松 聡子, 朴 杓允
    (一社)医学生物学電子顕微鏡技術学会, Nov. 2010, 医学生物学電子顕微鏡技術学会誌, 24(2) (2), 83 - 83, Japanese
    [Refereed]

  • Saki Shimoi, Kanako Inoue, Hiroko Kitagawa, Masanori Yamasaki, Seiya Tsushima, Pyoyun Park, Kenichi Ikeda
    We screened collagenolytic/gelatinolytic bacteria from rice leaves and soil which degraded the fungal extracellular matrix to establish a novel biological control measure inhibiting germling adhesion on the host plant surface against airborne fungal diseases such as rice blast disease Magnaporthe oryzae B. Coch. Two different screening methods, i.e., screening from the leaf-associated bacterial library and direct screening from leaf and soil with or without collagen incubation, were conducted. Screening from the collagen treated material resulted in a higher number of gelatinolytic isolates than without collagen treatment. The selected bacteria were identified as Acidovorax, Sphingomonas, Chryseobacterium, and Pseudomonas genera by 16S rDNA sequence. Based on treatment with EDTA and addition of divalent cations, four of the five screened isolates tested produced a metalloproteinase. Furthermore, the enzymes produced by Acidovorax and Sphingomonas sp. were categorized as calcium-dependent metalloproteinases, and the enzymes produced by Chryseobacterium sp. were categorized as calcium/zinc-dependent metalloproteinases. The screened bacterial culture showed inhibitory effects on spore adhesion on the plastic cover glass, and disease protective effects on rice. This study suggests that bacteria inhibiting germling adhesion by phytopathogenic fungi may have promise as a biological agent. (C) 2010 Elsevier Inc. All rights reserved.
    ACADEMIC PRESS INC ELSEVIER SCIENCE, Nov. 2010, BIOLOGICAL CONTROL, 55(2) (2), 85 - 91, English
    [Refereed]
    Scientific journal

  • Gang-Su Hyon, Ken-ichi Ikeda, Naoki Hosogi, Takeshi Shinogi, Pyoyun Park
    In the Japanese pear pathotype of Alternaria alternata, H(2)O(2) is generated solely from penetration pegs and not from other portions of subcuticular hyphae within the pectin layers of host leaves. A close association between H(2)O(2) generation and fungal aggressiveness is expected because the pegs are important for fungal penetration into the host epidermis. To determine the potential role of reactive oxygen species in microbial pathogenicity, we studied the inhibitory effects of the antioxidant reagent ascorbic acid and the NADPH oxidase inhibitor diphenylene iodonium on infection of the pathogen. In our study, we showed H(2)O(2) generation to be inhibited by inoculation with the mixture of ascorbic acid or diphenylene iodonium and spores at the pegs in the spore-inoculated host leaves. The decrease of generation in the pegs resulted in penetration failure, indicating that H(2)O(2) generation probably contributed to strengthening of the penetration and probably was recruited by NADPH oxidase.
    AMER PHYTOPATHOLOGICAL SOC, Sep. 2010, PHYTOPATHOLOGY, 100(9) (9), 840 - 847, English
    [Refereed]
    Scientific journal

  • O. Osman, H. Tanguichi, K. Ikeda, P. Park, S. Tanabe-Hosoi, S. Nagata
    Aims: To isolate and characterize copper-resistant halophilic bacteria from the polluted Maruit Lake, Egypt and identify the role of plasmids in toxic metal resistance. Methods and Results: We isolated strain MA2, showing high copper resistance up to the 1 center dot 5 mmol l-1 concentration; it was also resistant to other metals such as nickel, cobalt and zinc and a group of antibiotics. Partial 16S rRNA analysis revealed that strain MA2 belonged to the genus Halomonas. Copper uptake, measured by atomic absorption spectrophotometery, was higher in the absence of NaCl than in the presence of 0 center dot 5-1 center dot 0 mol l-1 NaCl during 5-15 min of incubation. Cell fractionation and electron microscopic observation clarified that most of the copper accumulated in the outer membrane and periplasmic fractions of the cells. Plasmid screening yielded two plasmids: pMA21 (11 kb) and pMA22 (5 kb). Plasmid curing resulted in a strain that lost both the plasmids and was sensitive to cobalt and chromate but not copper, nickel and zinc. This cured strain also showed weak growth in the presence of 0 center dot 5-1 center dot 0 mol l-1 NaCl. Partial sequencing of both plasmids led to the identification of different toxic metals transporters but copper transporters were not identified. Conclusions: The highest cell viability was found in the presence of 1 center dot 0 mol l-1 NaCl at different copper concentrations, and copper uptake was optimal in the absence of NaCl. Plasmid pMA21 encoded chromate, cobalt, zinc and cadmium transporters, whereas pMA22 encoded specific zinc and RND (resistance, nodulation, cell division) efflux transporters as well as different kinds of metabolic enzymes. Copper resistance was mainly incorporated in the chromosome. Significance and Impact of the Study: Strain MA2 is a fast and efficient tool for copper bioremediation and the isolated plasmids show significant characteristics of both toxic metal and antibiotic resistance.
    WILEY-BLACKWELL PUBLISHING, INC, Apr. 2010, JOURNAL OF APPLIED MICROBIOLOGY, 108(4) (4), 1459 - 1470, English
    [Refereed]
    Scientific journal

  • Water-involved resin blocks always give us bad thin-sections causing the poor cell images of biological tissues
    Meguro H, Inoue K, IKEDA KENICHI, Hyon GS, Hosogi N, Kanematsu S, Park P
    2010, Journal of Electron Microscopy Technology for Medicine and Biology, 23, 98, English
    International conference proceedings

  • Ultrastructural analysis of the papilla formation during plant-microbe interaction
    Miyata N, IKEDA KENICHI, Suzuki T, Murayama N, Wada M, Park P
    2010, Journal of Electron Microscopy Technology for Medicine and Biology, 23, 117, English
    International conference proceedings

  • Ultrastructural analysis of mycelial incompatibility in Rosellinia necatrix
    Inoue K, IKEDA KENICHI, Kanematsu S, Park P
    2010, Journal of Electron Microscopy Technology for Medicine and Biology, 24, 83, Japanese
    Symposium

  • Treatment of periodic acid may enhance the attachment ability between specimen surfaces and the ambient resin during ultrathin sectioning
    Shimoi S, IKEDA KENICHI, Kitaoka H, Inoue K, Hyon GS, Hosogi N, Kanematsu S, Park P
    2010, Journal of Electron Microscopy Technology for Medicine and Biology, 23, 97, English
    International conference proceedings

  • The occurrence of splits between plant surfaces and ambient epoxy resin is prevented by the oxidation of the epidermal cuticles with sodium perioxidate
    IKEDA KENICHI, Shimoi S, Kitaoka H, Morita Y, Hyon GS, Meguro H, Inoue K, Park P
    医学生物学電子顕微鏡技術学会, 2010, Journal of Electron Microscopy Technology for Medicine and Biology, 24(1) (1), 19 - 24, English
    [Refereed]
    Scientific journal

  • The dynamics of reactive oxygen species generation during sclerotia development in Sclerotinia minor
    IKEDA KENICHI, Osato T, Inoue K, Hyon GS, Hosogi N, Kanematsu S, Park P
    2010, Journal of Electron Microscopy Technology for Medicine and Biology, 23, 104, English
    International conference proceedings

  • Pathological roles of reactive oxygen species in plant pathogen
    Hyon GS, Morita Y, Kusaka M, Hosogi N, IKEDA KENICHI, Nakayashiki H, Park P
    2010, Journal of Electron Microscopy Technology for Medicine and Biology, 23, 86, English
    International conference proceedings

  • Oxygen-bubbly glutaraldehyde fixation can preserve mucoproteins or mucopolysaccharides in the vacuoles of plant cells
    Kitaoka H, IKEDA KENICHI, Inoue K, Kanematsu S, Park P
    2010, Journal of Electron Microscopy Technology for Medicine and Biology, 23, 100, English
    International conference proceedings

  • Mycelial incompatibility in Rosellinia necatrix
    Inoue K, IKEDA KENICHI, Kitazawa H, Hamanaka T, Ohta T, Kanematsu S, Park P
    2010, Journal of Electron Microscopy Technology for Medicine and Biology, 23, 117, English
    International conference proceedings

  • More than 0.2% water involved in unpolymerizaed Spurr's resin mixture causes sectioning defects in ultrathin sections of plant and fungal cells
    IKEDA KENICHI, Inoue K, Meguro H, Shimoi S, Kitaoka H, Morita Y, Hyon GS, Kanematsu S, Park P
    医学生物学電子顕微鏡技術学会, 2010, Journal of Electron Microscopy Technology for Medicine and Biology, 24(2) (2), 35 - 39, English
    [Refereed]
    Scientific journal

  • Fixation method for ultrastructural observation of fungal cells
    Ueno S, Inoue K, Morita Y, Kanematsu S, IKEDA KENICHI, Park P
    2010, Journal of Electron Microscopy Technology for Medicine and Biology, 24, 78, Japanese
    Symposium

  • Evaluation of the toxic effect of azoxystrobin on Botrytis cinerea
    Park P, Tsurumi T, Inoue K, Hyon GS, Hosogi N, Ishii H, Kanematsu S, IKEDA KENICHI
    2010, Journal of Electron Microscopy Technology for Medicine and Biology, 23, 104, English
    International conference proceedings

  • Comparison of electron staining method between uranyl acetate and hafnium chloride for biological specimen
    IKEDA KENICHI, Inoue K, Horiuchi Y, Kanematsu S, Park P
    2010, Journal of Electron Microscopy Technology for Medicine and Biology, 24, 79, Japanese
    Symposium

  • A novel staining method of methanolic hafnium chloride (MHC) for uranyl acetate; staining effect of MHC for thin sections of bacteria, fungi, human biopsis, rat tissues and plants
    Horiuchi Y, Onoe T, Inoue K, IKEDA KENICHI, Park P
    2010, Journal of Electron Microscopy Technology for Medicine and Biology, 23, 98, English
    International conference proceedings

  • A novel staining method for thin sections and en bloc tissues of rat kidney fixed with glutaraldehyde and osmium tetoxide using methanolic hafnium chloride
    Onoe T, Horiuchi Y, Inoue K, IKEDA KENICHI, Park P
    2010, Journal of Electron Microscopy Technology for Medicine and Biology, 23, 79, English
    International conference proceedings

  • Izumi Chuma, Takeshi Shinogi, Naoki Hosogi, Ken-ichi Ikeda, Hitoshi Nakayashiki, Pyoyun Park, Yukio Tosa
    The inner cellular structure of microconidia of Magnaporthe oryzae was examined using fluorescent probes and electron microscopic techniques. The volume of the nucleus relative to the cell was significantly larger in microconidia than in macroconidia or vegetative hyphae, similar to observations for spermatia of other fungi. Selective fluorescent staining revealed that cytosolic RNA was less abundant in microconidia than in macroconidia and germ tubes, suggesting that general metabolic activity in microconidia is low. Consistently, GFP expression driven by the TrpC promoter was highly active during the formation of phialides and microconidia but gradually decreased as the microconidia matured. Such data suggest that microconidia are in a quiescent or dormant state.
    SPRINGER TOKYO, Oct. 2009, JOURNAL OF GENERAL PLANT PATHOLOGY, 75(5) (5), 353 - 358, English
    [Refereed]
    Scientific journal

  • 植物－植物病原糸状菌相互作用における活性酸素種の役割
    IKEDA KENICHI, 朴杓允
    2009, Journal of Electron Microscopy Technology for Medicine and Biology, 23, 24 - 25, Japanese
    Symposium

  • The extracellular matrix produced from Alternaria alternata Japanese pear pathotype plays a possible role of adhesion on the surfaces of host leaves during plant infection
    Hyon GS, Muranaka Y, IKEDA KENICHI, Inoue K, Hosogi N, Meguro H, Yamada T, Hida S, Suzuki T, Park P
    医学生物学電子顕微鏡技術学会, 2009, Journal of Electron Microscopy Technology for Medicine and Biology, 23(1) (1), 1 - 8, English
    [Refereed]
    Scientific journal

  • Cytological characteristics of microconidia of Magnaporthe oryzae
    Chuma I, Shinogi T, Hosogi N, IKEDA KENICHI, Nakayashiki H, Park P, Tosa Y
    2009, Journal of General Plant Pathology, 75, 353 - 358, English
    [Refereed]
    Scientific journal

  • Ultrastructural identification of ion-leakage sites in host cells treated with AK-toxin I from Alternaria alternata Japanese pear pathotype
    Hosogi N, Hyon GS, Inoue K, Jiang S, IKEDA KENICHI, Park P
    2008, Journal of Electron Microscopy Technology for Medicine and Biology, 21, 172 - 178, English
    [Refereed]
    Scientific journal

  • Possible roles of extracellular matrix (ECM) in phytopathogenic fungus Magnaporthe oryzae
    IKEDA KENICHI, Inoue K, Park P
    2008, Korean Journal of Microscopy, 38, 1312 - 1313, English
    International conference proceedings

  • Pathological roles of reactive oxygen species at penetration pegs of Alternaria Japanese pear pathotype during plant infection
    Hyon GS, Morita Y, Hosogi N, IKEDA KENICHI, Nakayashiki H, Park P
    2008, Korean Journal of Microscopy, 38, 1235 - 1236, English
    International conference proceedings

  • Extracellular matrix of Magnaporthe oryzae may have a role in host adhesion during fungal penetration and is digested by matrix metalloproteinases
    Inoue K, Suzuki T, IKEDA KENICHI, Jiang S, Hosogi N, Hyon GS, Hida S, Yamada T, Park P
    2008, Journal of General Plant Pathology, 73, 388 - 398, English
    [Refereed]
    Scientific journal

  • Cytological study for pathological roles of compounds analogous to AK-toxins, disease determinants, during plant infection
    Hosogi N, Hyon GS, Osato T, Inoue K, IKEDA KENICHI, Park P
    2008, Korean Journal of Microscopy, 38, 1237 - 1238, English
    International conference proceedings

  • 植物感染における宿主・病原菌の細胞応答
    朴杓允, IKEDA KENICHI, 清水直人, 姜山, 細木直樹, 玄康洙, 井上加奈子
    日本植物病理学会, 2007, 植物‐病原微生物の相互作用のダイナミズム, 77 - 86, Japanese
    Symposium

  • A novel method for thin sections and en bloc tissues of rat kidney fixed with glutaralaldehyde and osmium tertoxide using methanolic hafnium chloride
    Horiuchi Y, Okumoto K, Akahoshi Y, Minamoto S, Ando M, Onoe T, IKEDA KENICHI, Park Pyoyun
    2007, Journal of Electron Microscopy Technology for Medicine and Biology, 21, 21 - 28, English
    [Refereed]
    Scientific journal

  • T Fukuhara, R Koga, N Aoki, C Yuki, N Yamamoto, N Oyama, T Udagawa, H Horiuchi, S Miyazaki, Y Higashi, M Takeshita, K Ikeda, M Arakawa, N Matsumoto, H Moriyama
    The International Committee on Taxonomy of Viruses (ICTV) recently accepted Endornavirus as a new genus of plant dsRNA virus. We have determined the partial nucleotide sequences of the RNA-dependent RNA polymerase regions from the large dsRNAs (about 14 kbp) isolated from barley (Hordeum vulgare), kidney bean (Phaseolus vulgaris), melon (Cucumis melo), bottle gourd (Lagenaria siceraria), Malabar spinach (Basella alba), seagrass (Zostera marina), and the fungus Helicobasidium mompa. Phylogenetic analyses of these seven dsRNAs indicate that these dsRNAs are new members of the genus Endornavirus that are widely distributed over the plant and fungal kingdoms.
    SPRINGER WIEN, May 2006, ARCHIVES OF VIROLOGY, 151(5) (5), 995 - 1002, English
    [Refereed]
    Scientific journal

  • K Ikeda, H Nakamura, N Matsumoto
    The white root rot fungus, Rosellinia necatrix, is a devastating soil-borne pathogen of many plant species. Biocontrol with the hypovirulence factor is promising, but disease symptoms, signs or culture morphology of the pathogen cannot be reliably used as markers for hypovirulence in this fungus. We attempted to obtain hypovirulent isolates from soil rather than from diseased roots, based on the hypothesis that hypovirulent isolates were more likely to persist in soil as saprobes. Sixteen isolates, belonging to eight mycelial compatibility groups (MCGs), were obtained from soil in two active and one abandoned Japanese pear orchards. Comparison of these isolates based on clonality revealed that six MCGs were commonly recovered from both diseased roots and soil and two MCGs exclusively from soil. No MCG was found in more than one orchard. With two exceptions, isolates within the same MCG were similar in virulence, competitive saprophytic ability (CSA) and mycelial growth rate whether or not they carried dsRNA. The two exceptional isolates recovered from soil had multiple dsRNA segments that caused hypovirulence, weakened CSA and restricted mycelial growth on nutrient-rich media. They belonged to different MCGs, each including dsRNA-free isolates. Isolates from soil contained various dsRNAs (44%), including the hypovirulence factor, more frequently than isolates from diseased roots in the same fields (25%), which is much higher than the proportion of isolates with dsRNA from diseased roots (19%) in a total of 424 isolates from Japan examined so far. These results suggest that isolation of R. necatrix from soil is an effective method to obtain isolates with dsRNAs, including the hypovirulence factor. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
    ELSEVIER SCIENCE BV, Oct. 2005, FEMS MICROBIOLOGY ECOLOGY, 54(2) (2), 307 - 315, English
    [Refereed]
    Scientific journal

  • Kouichi Suzaki, Ken-Ichi Ikeda, Atsuko Sasaki, Satoko Kanematsu, Naoyuki Matsumoto, Kouji Yoshida
    Monokaryotic strains of Helicobasidium mompa were used as vectors of a mycovirus between various H. mompa isolates to examine the transmissibility of one of the mycoviruses, totivirus (HmTV1-17 virus) in the hypovirulent isolate V17 of H. mompa. The isolates that acquired HmTV1-17 virus were also examined for any alteration in the virulence. Twelve dikaryotic isolates of H. mompa, belonging to 11 mycelial compatibility groups (MCGs) and being mycelially incompatible with isolate V17, were used as recipients of HmTV1-17 virus. Two monokaryotic isolates that were mycelially incompatible with isolate V17 and all of the recipients were also used as vectors of HmTV1-17 virus between isolate V17 and the recipients. When isolate V17 and recipients were directly paired on plate media, HmTV1-17 virus was transmitted from isolate V17 into 2 of the 12 recipients (i.e., 2 of the 11 MCGs). Two monokaryotic strains were paired with isolate V17, and the monokaryotic strains that acquired HmTV1-17 virus were then used as new virus donors. When the monokaryotic strains containing HmTV1-17 virus were paired with the 12 recipients, HmTV1-17 virus was transmitted into 7 of the 12 recipients from the monokaryotic strains (i.e., 7 of 11 MCGs). Based on these results, we concluded that monokaryotic strains could act as vectors to transmit HmTV1-17 virus into H. mompa isolates. When four of the H. mompa isolates that acquired HmTV1-17 virus were used to inoculate apple rootstock Malus prunifolia, the virulence of all of the isolates was attenuated from that of their parental isolates. Moreover, because the DNA fingerprints of the fungal isolates that acquired HmTV1-17 virus were the same as those of their parental isolates, the infection with HmTV1-17 virus is considered the cause of virulence attenuation of H. mompa. © The Phytopathological Society of Japan and Springer-Verlag 2005.
    Jun. 2005, Journal of General Plant Pathology, 71(3) (3), 161 - 168, English
    [Refereed]
    Scientific journal

  • K Ikeda, H Nakamura, M Arakawa, T Koiwa, N Matsumoto
    Eighty-three isolates of the violet root rot fungus, Helicobasidium mompa, were collected in a tulip tree plantation and analyzed for the dynamics of double-stranded (ds) RNA for five years. They were divided into eight mycelial compatibility groups (MCGs). Prevalent MCGs 60 and 68 included 61 and 11 isolates, respectively. Electrophoretic profiles of dsRNA in the first year collection of MCG 60 contained no or a single large dsRNA (more than 10 kb) with or without small dsRNAs (ca. 2.0-2.5 kb). Additional dsRNA fragments, i.e., a middle dsRNA (ca. 8.0 kb) or another type of small dsRNAs, became evident within MCG 60 isolates with time. Northern hybridization revealed the relatedness of all large and middle dsRNA fragments within MCG 60 but small fragments of dsRNA were variable. Large dsRNA fragment differed from that in other MCGs even in the same field. Correlation between specific dsRNA fragments and hypovirulence was not observed. Possible explanations for the accumulation of dsRNA fragments during the growth of disease patch by MCG 60 are discussed in terms of their internal changes such as evolution of novel dsRNA fragments from pre-existing viruses or fungal genomic DNA and horizontal transmissions. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
    ELSEVIER SCIENCE BV, Jan. 2005, FEMS MICROBIOLOGY ECOLOGY, 51(2) (2), 293 - 301, English
    [Refereed]
    Scientific journal

  • K Ikeda, H Nakamura, M Arakawa, N Matsumoto
    The diversity and vertical transmission of double-stranded (ds) RNA in Helicobasidium mompa and Rosellinia necatrix was examined by electrophoresis and Northern hybridization. These two fungi share the similar niche as root rot pathogens of trees in forests and orchards, and had diverse dsRNAs. The detection frequency of dsRNA in both fungi was different; in H. mompa, 68.4 % (132 out of 193 MCGs; mycelial compatibility groups) had dsRNA, whereas 20.9 % (53 out of 254 MCGs) in R. necatrix. dsRNA banding patterns and Northern blot analyses revealed the presence of various dsRNA elements in both fungi. Hyphal tip isolation was mostly unsuccessful to remove dsRNA with some exceptions. Sexual reproduction functioned to remove dsRNA in both fungi since dsRNA was not detected from single sexual spore cultures. Possible explanations for the difference in the detection frequency of dsRNA are discussed in terms of the differences in their sexual reproduction and other factors.
    CAMBRIDGE UNIV PRESS, Jun. 2004, MYCOLOGICAL RESEARCH, 108, 626 - 634, English
    [Refereed]
    Scientific journal

  • A reovirus causes hypovirulence of Rosellinia necatrix
    S Kanematsu, M Arakawa, Y Oikawa, M Onoue, H Osaki, H Nakamura, K Ikeda, Y Kuga-Uetake, H Nitta, A Sasaki, K Suzaki, K Yoshida, N Matsumoto
    White root rot, caused by Rosellinia necatrix, is a serious soilborne disease of fruit trees and other woody plants. R. necatrix isolate W370 contains 12 segments of double-stranded RNA (dsRNA) that is believed to represent a possible member of the family Reoviridae. W370 was weakly virulent and its hyphal-tip strains became dsRNA free and strongly virulent. The 12 segments of W370dsRNA were transmitted to hygromycin B-resistant strain RT37-1, derived from a dsRNA-free strain of W370 in all or none fashion through hyphal contact with W370. The W370dsRNA-transmitted strains were less virulent than their parent strain RT37-1 on apple seedlings, with mortality ranging between 0 to 16.7% in apple seedlings that were inoculated with the W370dsRNA-containing strains and 50 to 100% for seedlings inoculated with the dsRNA-free strains. Sonic W370dsRNA-containing strains killed greater than 16.7% of seedlings, but these were found to have lost the dsRNA in planta. These results indicate that W370dsRNA is a hypovirulence factor in R. necatrix. In addition, a strain lost one segment (S8) of W370dsRNA during subculture, and the S8-deficient mutant strain also exhibits hypovirulence in R. necatrix.
    AMER PHYTOPATHOLOGICAL SOC, Jun. 2004, PHYTOPATHOLOGY, 94(6) (6), 561 - 568, English
    [Refereed]
    Scientific journal

  • H Nakamura, K Ikeda, M Arakawa, T Akahira, N Matsumoto
    A violet root rot fungus recently found in Japan was identified as Helicobasidium brebissonii. The fungus was compared with another violet root rot fungus, H. mompa, which is distributed throughout Japan, in terms of morphological characteristics of the basidiospores and the conidial state, sequences of rDNA ITS regions, and pathogenicity on carrot, sweet potato and apple rootstock. The two species were clearly discriminated by these features and the taxonomic status of both species is discussed.
    CAMBRIDGE UNIV PRESS, Jun. 2004, MYCOLOGICAL RESEARCH, 108, 641 - 648, English
    [Refereed]
    Scientific journal

  • Ken-Ichi Ikeda, Hitoshi Nakamura, Naoyuki Matsumoto
    Apr. 2004, Journal of General Plant Pathology, 70(2) (2), 143, English
    Scientific journal

  • Repeat-induced point mutation (RIP) in Magnaporthe grisea: Evidence for the presence of sexual cycle in nature
    K Ikeda, H Nakayashiki, Y Tosa, S Mayama
    The RIP (repeat-induced point mutation) process introduces C:G to T:A transitions into duplicated sequences during a specific period in the sexual reproduction and has been reported in only two fungal species, Neurospora crassa and Podospora anserina. To investigate whether a RIP-like process operates in Magnaporthe grisea, the retrotransposon MAGGY and the hygromycin B phosphotransferase gene were introduced into the fungus as multiple transgenes and examined for sequence alterations after asexual growth and sexual crossing. Numerous C:G to T:A transitions were found in the transgenes, preferentially in (A/Tp)Cp(A/T) contexts, in the sexual progenies, suggesting that a RIP-like process functions in M. grisea. We also examined the sequence of another retrotransposon, Pyret, in field isolates of M. grisea. Even though the perfect stage rarely has been observed in M. grisea under field conditions, RIP-like transitions were found in all the field isolates tested and were correlated with the fertility of the isolates in Petri dishes. Analysis of RIP-like transitions in repetitive sequences may provide a tool to estimate the frequency of sexual recombination of M. grisea isolates.
    SPRINGER, 2004, RICE BLAST: INTERACTION WITH RICE AND CONTROL, 57 - 63, English
    [Refereed]
    International conference proceedings

  • K Ikeda, H Nakamura, N Matsumoto
    Helicobasidium mompa is a clampless basidiomycete and binucleate both in secondary and primary hyphae. Single basidiospore isolates were paired in sibling and non-sibling combinations. We did not observe any alterations in colony morphology, such as tuft formation, but mycelial incompatibility, as indicated by the presence of a dark demarcation line between colonies, occurred in a unifactorial manner. Techniques using DNA molecular markers, such as internal transcribed spacer-restriction fragment-length polymorphism (ITS-RFLP), inter-simple-sequence-repeat polymerase chain reaction (PCR), and universally primed PCR, failed to identify nuclear migration to opposite single basidiospore isolates in all but one of the 92 pairings. These results imply that single basidiospore isolates of H. mompa may be incompetent to mate under laboratory conditions and that a single mycelial incompatibility factor operates in single basidiospore isolates.
    CAMBRIDGE UNIV PRESS, Jul. 2003, MYCOLOGICAL RESEARCH, 107, 847 - 853, English
    [Refereed]
    Scientific journal

  • Hypovirulent strain of the violet root rot fungus Helicobasidium mompa
    IKEDA KENICHI
    2003, Journal of General Plant Pathology, 69(6) (6), 385 - 390, English
    [Refereed]
    Scientific journal

  • Helicobasidium mompa isolates from sweet potato in continuous monoculture fields
    IKEDA KENICHI
    2003, Journal of General Plant Pathology, 69, English
    [Refereed]
    Scientific journal

  • Repeat-induced point mutation (RIP) in Magnaporthe grisea: implications for its sexual cycle in the natural field context
    K Ikeda, H Nakayashiki, T Kataoka, H Tamba, Y Hashimoto, Y Tosa, S Mayama
    Repeat-induced point mutation (RIP) is a process that detects DNA duplications and peppers their sequences with C:G to T:A transitions in the sexual phase of the life cycle. So far, this unique mechanism has been identified as a currently active process in only two fungal species, Neurospora crassa and Podospora anserina. To determine whether a RIP-like process operates in the plant pathogenic fungus Magnaporthe grisea, the retrotransposon MAGGY and the hygromycin B phosphotransferase gene were introduced into the fungus as multiple transgenes and examined for sequence alterations after a cross. Frequent C:G to T:A transitions in the transgenes were found in the descendants, preferentially in (A/Tp)Cp(A/T)contexts, suggesting that a process similar to RIP functions in M. grisea. We also examined the sequence of another retrotransposon Pyret in six field isolates of M. grisea. Even though no perfect stage has been known in M. grisea under field conditions to date, RIP-like transitions were found in all the field isolates tested. Interestingly, the frequency of the transitions mostly correlated with the fertility of the isolates examined under laboratory conditions. These results imply that the sexual cycle of this fungus exists or existed in the natural field context.
    WILEY-BLACKWELL, Sep. 2002, MOLECULAR MICROBIOLOGY, 45(5) (5), 1355 - 1364, English
    [Refereed]
    Scientific journal

  • H. Nakamura, K. Ikeda, M. Arakawa, N. Matsumoto
    Conidiomata of the white root rot fungus were produced in axenic culture under near-ultraviolet light radiation. Pieces of sterilized Japanese pear twigs were placed on 7-day-old oatmeal agar culture in plates. The plates were further incubated for 5 days and then illuminated by near-ultraviolet light. Synnemata developed on the twigs within 5 weeks in 19 of 20 isolates tested, and conidia were observed in 12 of the 19 isolates. The synnemata and conidia produced were morphologically identical to those of Dematophora necatrix.
    Springer Japan, Jun. 2002, Mycoscience, 43(3) (3), 251 - 254, English
    [Refereed]
    Scientific journal

  • 池田 健一, 中村 仁, 松本 直幸
    日本土壌微生物学会, 2002, 土と微生物, 56(2) (2), 148 - 148, Japanese

  • Pyret, a Ty3/Gypsy retrotransposon in Magnaporthe grisea contains an extra domain between the nucleocapsid and protease domains
    H Nakayashiki, H Matsuo, Chuma, I, K Ikeda, S Betsuyaku, M Kusaba, Y Tosa, S Mayama
    A novel Ty3/Gypsy retrotransposon, named Pyret, was identified in the plant pathogenic fungus Magnaporthe grisea (anamorph Pyricularia oryzae). Pyret-related elements were distributed in a wide range of Pyricularia isolates from various gramineous plants. The Pyret element is 7250 bp in length with a 475 bp LTR and one conceptual ORF. The ORF contains seven nonsense mutations in the reading frame, indicating that the Pyret clone is lightly degenerate. Comparative domain analysis among retroelements revealed that Pyret exhibits an extra domain (WCCH domain) beyond the basic components of LTR retrotransposons. The WCCH domain consists of similar to 300 amino acids and is located downstream of the nucleocapsid domain. The WCCH domain is so named because it contains two repeats of a characteristic amino acid sequence, W-X-2-C-X-4-C-X-2-H-X-3-K. A WCCH motif-like sequence is found in the precoat protein of some geminiviruses, viral RNA-dependent RNA polymerase and also in an Arabidopsis protein of unknown function. Interestingly, detailed sequence analysis of the gag protein revealed that Pyret, as well as some other chromodomain-containing LTR retrotransposons, displays significant sequence homology with members of the gammaretroviruses (MLV-related retroviruses) in the capsid and nucleocapsid domains. This suggests that chromodomain-containing LTR retrotransposons and gammaretroviruses may share a common ancestor with the gag protein.
    OXFORD UNIV PRESS, Oct. 2001, NUCLEIC ACIDS RESEARCH, 29(20) (20), 4106 - 4113, English
    [Refereed]
    Scientific journal

  • K Ikeda, H Nakayashiki, M Takagi, Y Tosa, S Mayama
    MAGGY is a gypsy-like retrotransposon isolated from the plant pathogenic fungus Magnaporthe grisea. The ability of various stresses to activate MAGGY was tested in the original and in a heterologous host (Colletotrichum lagenarium), using beta -glucuronidase (GUS) as a reporter. The MAGGY promoter was activated in M. grisea by either heat shock, copper sulfate, or oxidative stress, but not by the antifungal substance p-coumaric acid. Transcriptional up-regulation of MAGGY RNA was also observed following heat shock and oxidative stress. The MAGGY promoter remained responsive to the above-mentioned stresses when transformed into a M. grisea isolate that had no endogenous MAGGY elements. In C. lagenarium, however, the MAGGY promoter showed only basal expression of GUS and no further up-regulation was induced by any of the stress treatments, suggesting that the stress-responding cis-elements) in the MAGGY promoter is not functional in a wider range of fungi. The relationship between the activation of MAGGY by stress and phenotypic diversification in M. grisea, including variations in pathogenicity, is discussed.
    SPRINGER-VERLAG, Oct. 2001, MOLECULAR GENETICS AND GENOMICS, 266(2) (2), 318 - 325, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Methylation is not the main force repressing the retrotransposon MAGGY in Magnaporthe grisea
    H Nakayashiki, K Ikeda, Y Hashimoto, Y Tosa, S Mayama
    We: have introduced the LTR-retrotransposon MAGGY into a naive genome of Magnaporthe grisea and estimated the copy number of MAGGY in a cell by serial isolation of fungal protoplasts at certain time intervals. The number of MAGGY elements rapidly-increased for a short period following introduction. However, it did not increase geometrically and reached equilibrium at 20-30 copies per genome, indicating that MAGGY was repressed or silenced during proliferation, De novo methylation of MAGGY occurred immediately following invasion into the genome but the degree of methylation was constant and did not correlate with the repression of MABGGY. 5-Azacytidine treatment demethylated and transcriptionally activated the MAGGY element in regenerants but did not affect transpositional frequency, suggesting that post-transcriptional suppression, not methylation, is the main force that represses MAGGY proliferation in M.grisea, Support for this conclusion was also obtained by examining the methylation status of MAGGY sequences in field isolates of M.grisea with active or inactive MAGGY elements. Methylation of the MAGGY sequences was detected in some isolates but not in others, However, the methylation status did not correlate with the copy numbers and activity of the elements.
    OXFORD UNIV PRESS, Mar. 2001, NUCLEIC ACIDS RESEARCH, 29(6) (6), 1278 - 1284, English
    [Refereed]
    Scientific journal

  • Comparative analyses of the distribution of various transposable elements in Pyricularia and their activity during and after the sexual cycle
    Y Eto, K Ikeda, Chuma, I, T Kataoka, S Kuroda, N Kikuchi, LD Don, M Kusaba, H Nakayashiki, Y Tosa, S Mayama
    We examined the distribution and activity of six transposable elements found in the blast fungus, Pyricularia spp. Sixty-eight isolates from various gramineous plants were used for the survey, and the elements were plotted on a dendrogram constructed on the basis of their rDNA-ITS2 sequences. MGR586 and Pot2 (Class II elements), Mg-SINE (SINE-like element) and MGR583 (LINE-like retrotransposon) were widely distributed among the Pyricularia isolates, suggesting that they are old elements which arose in, or invaded, the Pyricularia population at very early stages in its evolution. By contrast, the distribution of the LTR-retrotransposons MAGCY and Grasshopper was limited or sporadic, suggesting that they are relatively new elements which recently invaded the Pyricularia population by means of horizontal transfer events. The activity of these elements was evaluated by Southern analysis in progenies derived from a cross between a Setaria isolate and a Triticum isolate. Many new MAGGY signals were observed, which were absent in the parental isolates, at various stages of the sexual cycle and following vegetative growth. In contrast, the other elements yielded few, if any, such signals. Analysis of the sequences flanking the new MAGGY insertions revealed that they were each associated with a 5-bp target-site duplication at both ends of the insertion. These data suggested that MAGGY was the most active of the elements tested for transposition in Pyricularia.
    SPRINGER-VERLAG, Jan. 2001, MOLECULAR AND GENERAL GENETICS, 264(5) (5), 565 - 577, English
    [Refereed]
    Scientific journal

  • H Nakayashiki, N Nishimoto, K Ikeda, Y Tosa, S Mayama
    The LTR-retrotransposon MAGGY is found sporadically in isolates of Pyricularia grisea (Magnaporthe grisea). Based on a dendrogram constructed by RFLP analysis of rDNA, isolates that carry MAGGY elements were classified into a single cluster that comprised four rDNA types. However, in a few members of this cluster, exemplified by isolates from common millet (Panicum miliaceum), the MAGGY element has distinct features. Southern analysis suggested that these isolates possessed a single copy of a MAGGY-related sequence whose restriction map differed from that of MAGGY itself. Sequence analysis revealed that the MAGGY-related sequence was a degenerate form of MAGGY, characterized by numerous C:G to T:A transitions, which have often been reported to result from RIP (Repeat-induced point mutation) or RIP-like processes. However, the favored target site for C:G to T:A transitions in this fungus, determined by examining a total of 501 sites, was (A/T)pCp(A/T), which differs from that for the RIP process originally reported in Neurospora (CpA), and from that reported in Aspergillus (CpC). The fact that certain members of the cluster of MAGGY carriers retain a single copy of a degenerate MAGGY element implies that the ancestor of these isolates successfully "captured" the invading MAGGY element.
    SPRINGER VERLAG, Jul. 1999, MOLECULAR AND GENERAL GENETICS, 261(6) (6), 958 - 966, English
    [Refereed]
    Scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • Genetical and physiological studies on the species-specific parasitism of Magnaporthe grisea in gramineous plants with a special reference to a cross between the Triticum isolate and the Setaria isolate
    S Mayama, Y Tosa, H Nakayashiki, M Kusaba, T Kataoka, W Kishimoto, J Murakami, K Kawashima, Y Sesumi, N Kikuchi, LD Don, K Ikeda, T Tsurushima
    Pyricularia grisea, the blast fungus of many gramineous plants, includes several host-specific subgroups, A phenogram constructed from rDNA-RFLP data showed that subgroups pathogenic on crops (i.e., rice, foxtail millet, common millet; finger millet, and wheal) and the subgroup pathogenic on crabgrass were classified into different clusters and that this fungeal species has a high diversity. The distribution of MAGGY on the phenogram suggested that this retrotransposon was acquired by a common ancestor of four rDNA types including Oryza isolates (pathogenic oil rice) and Setaria isolates (pathogenic on foxtail millet). To elucidate mechanisms of the species-specific parasitism in gramineous crops classified as a subgroup; we crossed a Setaria isolate with a Triticum isolate (pathogenic on wheat and with no MAGGY), and produced an F-1 population which (i) showed segregation in pathogenicity, phytotoxic compounds, and MAGGY copies, (ii) included mutants in mycelial color: appressorium formation, pathogenicity, and phytotoxic compounds. and (iii) contained many transposed copies of MAGGY. The usefulness of this population for analyses of the species-specific parasitism was discussed.
    SPRINGER, 1998, MOLECULAR GENETICS OF HOST-SPECIFIC TOXINS IN PLANT DISEASES, 13, 211 - 221, English
    [Refereed]
    International conference proceedings

■ MISC
  • Evidence that Pyricularia fungi have an underground life cycle in nature
    XIANG Zikai, 中屋敷均, 池田健一
    2024, 日本植物病理学会大会プログラム・講演要旨予稿集, 2024

  • Analysis of fungal localization during disease development caused by wheat seeds contaminated with Pyricularia oryzae.
    奥田樹, 内橋嘉一, 中屋敷均, 土佐幸雄, 池田健一
    2024, 日本植物病理学会報, 90(1) (1)

  • Analysis of factors involved in growth inhibition and blackening of roots infected with Pyricularia grisea
    XIANG Zikai, 鶴嶋鉄, 中屋敷均, 池田健一
    2023, 日本植物病理学会大会プログラム・講演要旨予稿集, 2023

  • Effect of ripening stages on the frequency of seed contamination in wheat spikes inoculated with Pyrizularia oryzae
    池田健一, 内橋嘉一, 奥田樹, 中屋敷均, 土佐幸雄
    2023, 日本植物病理学会大会プログラム・講演要旨予稿集, 2023

  • In vitro production of wheat blast (Pyricularia oryzae pathotype Triticum)-infected seeds from healthy wheat seeds.
    奥田樹, 内橋嘉一, 中屋敷均, 土佐幸雄, 池田健一
    2023, 日本植物病理学会報, 89(1) (1)

  • Techniques for electron microscopic observation in the analysis of plant-microbe interactions
    Kenichi Ikeda
    Lead, Oct. 2022, Journal of Electron Microscopy Technology for Medicine and Biology, 35(1) (1), 16 - 19, Japanese
    [Invited]

  • Inhibitory effects of benomyl to the wheat blast fungus.
    内橋嘉一, 池田健一, 奥田樹, 川口藍乃, 松本純一, 足助聡一郎, 神頭武嗣, 土佐幸雄
    2022, 日本植物病理学会報, 88(1) (1)

  • Race-cultivar specific resistance against Pyricularia oryzae on roots is not as effective as on leaves
    XIANG Zikai, 中屋敷均, 池田健一
    2022, 日本植物病理学会大会プログラム・講演要旨予稿集, 2022

  • Cross-talk between sugar and lipid metabolisms during the infection process of Pyricularia oryzae.
    YANG J., XIANG Z., 中屋敷均, 池田健一
    2022, 日本植物病理学会報, 88(1) (1)

  • Mechanisms of root infection in Pyricularia oryzae and P. grisea (1) Characterization of responses of gramineous plants to infection by various P. oryzae and P. grisea pathotypes.
    XIANG Z.K., 岡田大樹, 中屋敷均, 池田健一
    2022, 日本植物病理学会報, 88(1) (1)

  • Evaluation of primers for quantitative PCR and LAMP to detect the Triticum pathotype of Pyricularia oryzae.
    池田健一, 内橋嘉一, 川口藍乃, 中屋敷均, 土佐幸雄
    2021, 日本植物病理学会報, 87(1) (1)

  • Growth Inhibitory effects of pefurazoate and ipconazole to Wheat Blast Fungus
    内橋嘉一, 池田健一, 川口藍乃, 松本純一, 森田耕平, 足助聡一郎, 神頭武嗣, 土佐幸雄
    2021, 日本植物病理学会大会プログラム・講演要旨予稿集, 2021

  • Novel Efficient Primers for qPCR and LAMP to detect the Triticum pathotype of Pyricularia oryzae
    池田健一, 内橋嘉一, 奥田樹, 中屋敷均, 土佐幸雄
    2021, 日本植物病理学会大会プログラム・講演要旨予稿集, 2021

  • Cell biology in phytopathogenic fungi during host infection: commonalities and differences
    IKEDA KENICHI, PARK Pyoyun, NAKAYASHIKI HITOSHI
    May 2019, Journal of General Plant Pathology, 85(3) (3), 163 - 173, English
    [Refereed]
    Introduction scientific journal
    Link to Kobe Univ. Repository (Kernel)

  • An Argonaute gene in the fungus Magnaporthe oryzae functions as a negative regulator of RNA silencing
    Akihide Iritani, Nguyen Quyet, Van Vu Ba, Shuhei Ohkita, Kana Yokoya, Ken-ichi Ikeda, Hitoshi Nakayashiki
    GENETICS SOC JAPAN, Dec. 2016, GENES & GENETIC SYSTEMS, 91(6) (6), 360 - 360, English
    Summary international conference

  • NADPH-dependent alkenal/one oxidoreductase (AOR) supports the growth and protect from oxidative stress in Arabidopsis thaliana
    Takagi Daisuke, Kentaro Ifuku, Hironori Inoue, Masahiro Tamoi, Ken-ichi Ikeda, Kanako Ikeda Inoue, Hiroshi Fukayama, Chikahiro Miyake
    Mar. 2015, 第56回日本植物生理学会年会, Japanese
    Summary national conference

  • 明所高温条件で誘導されるステート2によるPSII障害回避機構の解析
    丸谷曜子, 山内靖雄, 東山真理, 秋本誠志, 井上加奈子, 池田健一, 水谷正治, 杉本幸裕
    2015, 日本植物生理学会年会要旨集, 56th

  • リアルタイム定量PCR法による菌核病生物防除剤Coniothyrium minitansの特異的検出(2013年度大会一般講演要旨)
    宿南 良, 岩本 豊, 小川 宗和, 池田 健一
    日本土壌微生物学会, 01 Apr. 2014, 土と微生物, 68(1) (1), 52 - 52, Japanese

  • 光・温度条件の変化により形成されるPSI-LHCII超複合体の解析
    丸谷曜子, 山内靖雄, 井上加奈子, 池田健一, 水谷正治, 杉本幸裕
    2014, 日本農芸化学会関西支部講演会講演要旨集, 486th

  • 病原菌をウイルスで病気にさせる生物防除法：ヴァイロコントロール
    IKEDA KENICHI
    秋田今野商店, 2013, 温古知新, 50(50) (50), 120 - 126, Japanese
    [Invited]
    Introduction scientific journal

  • 病原菌のアキレス腱をターゲットとした病害防除戦略
    IKEDA KENICHI
    2012, 作物研究, 57, 61 - 66, Japanese
    [Invited]
    Introduction scientific journal

  • (153) Cytological Analysis of noxA and noxB Mutants of Alternaria alternata Japanese Pear Pathotype during Infection Processes(Abstracts of the Papers Presented at the Annual Meeting of the Society)
    Hyon G.-S., Morita Y., Ikeda K., Nakayashiki H., Park P.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2008, Annals of the Phytopathological Society of Japan, 74(3) (3), 207 - 207, Japanese

  • (2) Pathological Roles of Reactive Oxygen Species during the Sclerotial Development in Sclerotinia minor(Abstracts of the Papers Presented at the Annual Meeting of the Society)
    Osato T., Ikeda K., Park P.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2008, Annals of the Phytopathological Society of Japan, 74(3) (3), 167 - 167, Japanese

  • (140) The Effects on Natural Nutrients during Spore Germination in Magnaporthe oryzae(Abstracts of the Papers Presented at the Annual Meeting of the Society)
    Inoue K., Ikeda K., Park P.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2008, Annals of the Phytopathological Society of Japan, 74(3) (3), 204 - 204, Japanese

  • (20)Cytosine Methylation in Magnaporthe Oryzae is Dispensable for the Life Cycle of the Fungus in Nature(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)
    Ikeda K., Tanaka M., Murata T., Shiina K., Tosa Y., Mayama S., Park P., Nakayashiki H.
    The Phytopathological Society of Japan (PSJ), 20 Feb. 2008, Annals of the Phytopathological Society of Japan, 74(1) (1), 61 - 61, Japanese

  • (13)Functional Analysis of NoxB, a Gene Involved in Reactive Oxygen Species (ROS) Generation, in Alternaria alternata Japanese Pear Pathotype(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)
    Hyon G. S., Morita Y., Shinogi T., Ikeda K., Nakayashiki H., Park P.
    The Phytopathological Society of Japan (PSJ), 20 Feb. 2008, Annals of the Phytopathological Society of Japan, 74(1) (1), 59 - 59, Japanese

  • (23)The Ability of Adherence during Spore Germination in Magnaporthe oryzae(Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan, 2007)
    Inoue K., Ikeda K., Park P.
    The Phytopathological Society of Japan (PSJ), 20 Feb. 2008, Annals of the Phytopathological Society of Japan, 74(1) (1), 62 - 62, Japanese

  • Kanako Inoue, Tomoko Suzuki, Kenichi Ikeda, Shan Jiang, Naoki Hosogi, Gang-Su Hyon, Sizuka Hida, Taku Yamada, Pyoyun Park
    SPRINGER TOKYO, Feb. 2008, JOURNAL OF GENERAL PLANT PATHOLOGY, 74(1) (1), 96 - 96, English
    Others

  • Pyoyun Park, Ken-ichi Ikeda
    Cellular responses in fungi and in susceptible or resistant hosts during fungus-plant interactions have been studied ultrastructurally to examine their role in pathogenicity. Pathogenicity is determined in some saprophytic fungi by various factors: the production of disease determinants such as the production of host-specific toxins (HSTs) or the extracellular matrix (ECM) by fungal infection structures and H(2)O(2) generation from penetration pegs. Three different target sites for HSTs have been identified in host cells in many ultrastructural studies: plasma membranes, chloroplasts, and mitochondria. The mode of action of HSTs is characterized by the partial destruction of the target structures only in susceptible genotypes of host plants, with the result that the fungus can colonize the host. The infection structures of most fungal pathogen secrete ECM on plant surfaces during fungal differentiation, while the penetration pegs of some pathogens produce reactive oxygen species (ROS) in the cell walls and plasma membranes. The pathological roles of ECM and H(2)O(2) generation are discussed here in light of ultrastructural evidence. Host and fungal characteristics in the incompatible interactions include the rapid formation of lignin in host epidermal cell walls, failure of penetration pegs to invade lignin-fortified pectin layers, the inhibition of subcuticular hyphal proliferation and the collapse of hyphae that have degraded cell walls within pectin layers of the host. Apoptosis-like host resistant mechanism is also discussed.
    SPRINGER TOKYO, Feb. 2008, JOURNAL OF GENERAL PLANT PATHOLOGY, 74(1) (1), 2 - 14, English
    [Refereed]
    Book review

  • (138) Functional Analysis of NoxA, a Gene Involved in Reactive Oxygen Species (ROS) Generation on the Penetration peg of Alternaria alternata Japanese Pear Pathotype(Abstracts of the Papers Presented at the Annual Meeting of the Society)
    Hyon G.-S., Shinogi T., Ikeda K., Nakayashiki H., Park R.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2007, Annals of the Phytopathological Society of Japan, 73(3) (3), 211 - 211, Japanese

  • (85) Trapping Mycoviruses of Rosellinia necatrix(Abstracts of the Papers Presented at the Annual Meeting of the Society)
    Furuya N., Ide Y., Nakamura H., Ikeda K., Matsumoto N.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2007, Annals of the Phytopathological Society of Japan, 73(3) (3), 197 - 197, Japanese

  • (98) Effects of various inhibitor treatments on the infection structures settlement of Magnaporthe oryzae(Abstracts of the Papers Presented at the Annual Meeting of the Society)
    Inoue K., Ikeda K., Park R.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2007, Annals of the Phytopathological Society of Japan, 73(3) (3), 200 - 201, Japanese

  • いもち病菌小型分生子の病理学的役割を解明するための基礎調査
    細木直樹, 中馬いづみ, 篠木武, 池田健一, 中屋敷均, 眞山滋志, 土佐幸雄, PARK P
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2007, 日本植物病理学会報, 73(3) (3), 213 - 213, Japanese

  • Ken-Ichi Ikeda
    Dec. 2006, Journal of General Plant Pathology, 72(6) (6), 408 - 409, English
    [Invited]
    Book review

  • (98) Cytological Study for the Inhibiting Effects of Glycoprotein-digesting Enzymes on the Host Adhesion of Magnaporthe oryzae Infection Structures(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)
    Inoue K., Jiang S., Hosogi N., Hyon G., Yu Y., Park P., Ikeda K.
    The Phytopathological Society of Japan (PSJ), 25 Nov. 2006, Annals of the Phytopathological Society of Japan, 72(4) (4), 227 - 228, Japanese

  • Identification of the Gene Responsible for Methylation of the Retrotransposon MAGGY in Magnaporthe oryzae (Abstracts Presented at the Meeting of the Kansai Division,Abstracts of Papers Presented at the Division Meetings of the Phytopathological Society of Japan 2005)
    Shiina K., Ikeda K., Kadotani N., Nakayashiki H., Tosa Y., Mayama S.
    The Phytopathological Society of Japan (PSJ), 25 Feb. 2006, Annals of the Phytopathological Society of Japan, 72(1) (1), 68 - 68, Japanese

  • 紋羽病菌におけるdsRNAの多様性とその動態
    IKEDA KENICHI
    2006, 日本植物病理学会報, 72, 181, Japanese
    [Invited]
    Introduction scientific journal

  • (260) Detection of Mycoviruses (Double-stranded RNAs) from Rosellinia necatrix Isolates from Soil Obtained by the Baiting Technique(Abstracts of the Papers Presented at the 2005 Annual Meeting in Shizuoka)
    Ikeda K., Nakamura H., Matsumoto N.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2005, Annals of the Phytopathological Society of Japan, 71(3) (3), 254 - 255, Japanese

  • 病原力低下因子の探索と評価 (特集 病原力低下因子利用による果樹類紋羽病の遺伝子治療)
    中村 仁, 池田 健一
    日本植物防疫協会, Feb. 2004, 植物防疫, 58(2) (2), 50 - 53, Japanese

  • Screening for hypovirulence factor in violet and white root rot fungi
    IKEDA KENICHI, 中村 仁
    2004, 植物防疫, 58, 50 - 53, Japanese
    [Invited]
    Introduction scientific journal

  • Diversification of dsRNAs in Helicobasidium mompa with the Expansion of Mycelial Compatibility Groups(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)
    Ikeda K., Nakamura H., Matsumoto N.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2003, Annals of the Phytopathological Society of Japan, 69(3) (3), 277 - 278, Japanese

  • Pathogenicity of a Violet Root Rot Fungus, Helicobasidium brebissonii(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)
    Nakamura H., Ikeda K., Akahira T.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2003, Annals of the Phytopathological Society of Japan, 69(3) (3), 277 - 277, Japanese

  • Double-stranded RNA in an Isolate of the Violet Root Rot Fungus, Helicobasidium mompa, as a Hypovirulence Factor(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)
    Ikeda K., Nakamura H., Matsumoto N.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2003, Annals of the Phytopathological Society of Japan, 69(3) (3), 277 - 277, Japanese

  • Relationship between the Expansion of Disease Patch and Virulence of Mycelial Compatibility Groups in the Violet Root Rot Fungus, Helicobasidium mompa
    Nakamura H., Ikeda K., Uetake Y., Matsumoto N.
    リンゴ園およびユリノキ植栽林では,紫紋羽病菌,Helicobasidium mompaの特定のMCG(Mycelial Compatibility Group)がパッチ状に大きな分布域を示し,これらのみが数年後さらに大きく進展していたことを報告した(中村ら,2001).今回,新たに茨城県のクワ園における個体群構造の経年変化を調査したところ,クワ園においても大きな分布域を示すMCGが存在し,それらは2年後さらに大きく進展していた.次に,上記の3調査地で認められた各MCGに属する菌株を用いて接種試験を行い,MCGの分布域の大きさと病原力との関係について調べた.1MCG当たり1-3菌株をリンゴ台木苗に接種した結果,大きな分布域を示すMCGは相対的に強い病原力を示し,一方分布域の小さなMCGの病原力は強弱様々であった.また接種苗根上での菌糸塊形成頻度と病原力との間には正の相関が認められた.以上から,MCGの病原力は各調査地内での各MCGの分布域の大きさあるいは進展程度を決定する要困の1つであると判断された.
    The Phytopathological Society of Japan (PSJ), 25 Feb. 2003, Annals of the Phytopathological Society of Japan, 69(1) (1), 44 - 45, Japanese

  • (77)Pyret, a Ty3/Gypsy Retrotransposon in Magnaporthe grisea Contains an Extra Domain between the Nucleocapsid and Protease Domains
    Nakayashiki H., Matuo H., Chuma I., Ikeda K., Betsuyaku S., Kusaba M., Tosa Y., Mayama S.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2002, Annals of the Phytopathological Society of Japan, 68(2) (2), 176 - 177, Japanese

  • (317)Transmission of Double-Stranded RNA (dsRNA) through the Life Cycle in Helicobasidium mompa and Rosellinia necatrix
    Ikeda K., Nakamura H., Arakawa M., Matsumoto N.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2002, Annals of the Phytopathological Society of Japan, 68(2) (2), 241 - 241, Japanese

  • (169)Effect of Unsterile Soil Used in Pot Experiments on the Severity of Violet Root Rot Helicobasidium mompa
    Matsumoto N., Nakamura H., Ikeda K.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2002, Annals of the Phytopathological Society of Japan, 68(2) (2), 201 - 201, Japanese

  • (135)Another Violet Root Rot Fungus, Helicobasidium brebissoii(Desm.) Donk, Collected in Aomori
    Nakamura H., Ikeda K., Arakawa M., Akahira T., Matsumoto N.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2002, Annals of the Phytopathological Society of Japan, 68(2) (2), 192 - 192, Japanese

  • Production of Stromata of White Root Rot Fungus on Diseased Roots and Virulence of Single Ascospore Isolates(Abstracts Presented at the Meeting of the Kanto Division)
    Nakamura H., Ikeda K., Arakawa M., Matsumoto N.
    The Phytopathological Society of Japan (PSJ), 25 Apr. 2002, Annals of the Phytopathological Society of Japan, 68(1) (1), 68 - 68, Japanese

  • Occurrence of RIP(Repeat Induced Point Mutation)during Hybridization in Magnaporthe grisea
    Ikeda K., Nakayashiki H., Tosa Y., Mayama S.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2001, Annals of the Phytopathological Society of Japan, 67(2) (2), 130 - 130, Japanese

  • Methylation is not the Responsible Mechanism Repressing the Retrotransposon MAGGY in Magnaporthe grisea
    Nakayashiki H., Ikeda K., Hashimoto Y., Tosa Y., Mayama S.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2001, Annals of the Phytopathological Society of Japan, 67(2) (2), 129 - 130, Japanese

  • Degeneration of Transposable Elements by RIP (Repeat-Induced Point Mutation)-Like Process in Panicum Isolates of Pyricularia grisea RIP (repeat-induced point mutation)
    Ikeda K., Nakayashiki H., Tosa Y., Mayama S.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 2000, Annals of the Phytopathological Society of Japan, 66(2) (2), 120 - 120, Japanese

  • Activation of Transposition of MAGGY in Pyricularia oryzae : Analysis of Promoter Activity Using an LTR-GUS Construct
    Takagi M., Ikeda K., Nakayashiki H., Tosa Y., Mayama S.
    The Phytopathological Society of Japan (PSJ), 25 Jun. 1999, Annals of the Phytopathological Society of Japan, 65(3) (3), 341 - 342, Japanese

  • (5) Studies on Species-specific Parasitism of Magnaporthe grisea (5) Evidence for Transposition of MAGGY and Evaluation of Activity of Other Repetitive Elements in Progenies from the Cross, a Setaria Isolate × a Triticum Isolate (Abstracts Presented at the Meeting of the Kansai Division, 1998)
    Eto Y., Chuma I., Ikeda K., Tosa Y., Nakayashiki H., Mayama S.
    The Phytopathological Society of Japan (PSJ), 25 Dec. 1998, Annals of the Phytopathological Society of Japan, 64(6) (6), 616 - 616, Japanese

  • (99) Distribution of Transposable Elements and Double-stranded RNA in Pyricularia spp.
    Ikeda K., Chuma I., Eto Y., Kusaba M., Nakayashiki H., Tosa Y., Mayama S.
    The Phytopathological Society of Japan (PSJ), 25 Aug. 1998, Annals of the Phytopathological Society of Japan, 64(4) (4), 351 - 352, Japanese

■ Books And Other Publications
  • 病原性にかかわる遺伝子
    IKEDA KENICHI, TOSA YUKIO
    Others, 朝倉書店, 2013, Japanese
    Scholarly book

  • 病原菌を剥離させるアプローチによるイネいもち病生物防除法の開発
    IKEDA KENICHI
    Others, バイオコントロール研究会, 2012, Japanese
    Scholarly book

  • 花粉の世界をのぞいてみたら
    IKEDA KENICHI
    NTS, 2012, Japanese
    General book

  • Extracellular matrix (ECM) in phytopathogenic fungi: its role and potential target for disease protection
    IKEDA KENICHI, Inoue K, Kitagawa H, Meguro H, Shimoi S, Park P
    Joint work, INTECH Open Acess Publisher, 2012, English
    Scholarly book

  • Repeat-induced point mutation (RIP) in Magnaporthe grisea: evidence for the presence of sexual cycle in nature
    IKEDA KENICHI, Nakayashiki Hitoshi, Tosa Yukio, Mayama Shigeyuki
    Joint work, Kluwer Academic Publishers, 2004, English
    Scholarly book

■ Lectures, oral presentations, etc.
  • SDHI剤耐性ブロッコリー黒すす病菌の特性解析
    西村文宏, 片山貴博, 池田健一
    令和７年度日本植物病理学会大会, Mar. 2025, Japanese
    Oral presentation

  • いもち病菌の条件的ヘテロクロマチンは性格の異なる二つの領域から構成されている
    森本熱海, Thach Dang, 小林奈月, 池田健一, 中屋敷均
    令和７年度日本植物病理学会大会, Mar. 2025, Japanese
    Oral presentation

  • 遺伝子喪失に基づくゲノム比較法から見出したCACP3は付着器からの物理侵入に関わる分泌タンパク質である
    碓井遥香, 中山裕之進, 森結香, 重吉沙衣, 住田卓也, 入江俊一, 池田健一, 泉津弘佑
    令和７年度日本植物病理学会大会, Mar. 2025, Japanese
    Oral presentation

  • いもち病菌の根への感染機構の解明（４）：メヒシバいもち病菌の二次代謝物質合成に関わるPgACE1はイネ根の黒変症状に関与する
    向子愷, 中屋敷均, 池田健一
    令和７年度日本植物病理学会大会, Mar. 2025, Japanese
    Oral presentation

  • いもち病菌の新規マイコウイルスPoUV1のin vitro転写による感染系の確立
    村上敦哉, 齋藤瑠衣, 池田健一, 中屋敷均
    令和６年度日本植物病理学会関西部会, Sep. 2024, Japanese
    Oral presentation

  • 白紋羽病菌における分生子吻合管形成の特徴付け
    坂上千洋, 坂口耕太, 畑中郁穂, 兼松聡子, 中屋敷均, 池田健一
    令和６年度日本植物病理学会関西部会, Sep. 2024, Japanese
    Oral presentation

  • タマネギべと病菌の一次伝染株発病予防効果の検証
    西村文宏, 池田健一, 片山貴博
    令和６年度日本植物病理学会関西部会, Sep. 2024, Japanese
    Oral presentation

  • メヒシバ根より分離されたCorynascella humicolaのイネ科植物における生長促進および抵抗性誘導効果
    向子愷, 中屋敷均, 池田健一
    令和６年度日本植物病理学会関西部会, Sep. 2024, English
    Oral presentation

  • Stenotrophomonas maltophiliaは酸化ストレスを緩和しいもち病菌の感染を助長する
    松原くるみ, 小山舜平, 中屋敷均, 池田健一
    令和６年度日本植物病理学会関西部会, Sep. 2024, Japanese
    Oral presentation

  • コムギいもち病菌におけるヒストン修飾H4K20me3の機能解析
    森本熱海, 山﨑聖翔, Thach An Dang, 小林奈月, 池田健一, 中屋敷均
    令和６年度日本植物病理学会関西部会, Sep. 2024, Japanese
    Oral presentation

  • Transcriptome analysis of Pyricularia oryzae high-temperature response during appressorium formation
    Thanathip Sutthiphai, Sayuki Hiraiwa, Hitoshi Nakayashiki, Kenichi Ikeda, Chatchawan Jantasuriyara
    International Conference of the Genetics Society of Thailand 2024, Jun. 2024, English
    Public symposium

  • イプコナゾール及びベノミルはコムギいもち病菌汚染種子からの発病を抑制する
    内橋嘉一, 池田健一, 吉岡資洋, 原田正志, 足助聡一郎, 土佐幸雄
    日本植物病理学会大会, Mar. 2024, Japanese
    Oral presentation

  • いもち病菌の各種ヒストン修飾は各ゲノムコンパートメントで異なった相互作用をすることでゲノムの健全性を維持する
    An Thach Dang, 森本熱海, 小林奈月, 池田健一, 中屋敷均
    日本植物病理学会大会, Mar. 2024, English
    Oral presentation

  • 感染環境を模した培養条件下におけるいもち病菌の遺伝子発現を制御するヒストン修飾
    森本熱海, 馬場智子, An Thach Dang, 小林奈月, 池田健一, 中屋敷均
    日本植物病理学会大会, Mar. 2024, Japanese
    Oral presentation

  • ブロッコリー黒すす病菌に感染した種子が潜伏感染した苗を作り出す可能性
    西村文宏, 片山貴博, 池田健一
    日本植物病理学会大会, Mar. 2024, Japanese
    Oral presentation

  • 一部のいもち病菌は自然界で土壌中の生活環を有する
    向子愷, 中屋敷均, 池田健一
    日本植物病理学会大会, Mar. 2024, Japanese
    Oral presentation

  • 接種法の異なるコムギいもち病菌汚染種子の特性評価
    奥田樹, 内橋嘉一, 中屋敷均, 土佐幸雄, 池田健一
    日本植物病理学会大会, Mar. 2024, Japanese

  • 遺伝子喪失に基づくゲノム比較法から見出したCACP1は植物病原菌で進化的に保存された付着器侵入因子である
    森結香, 中山裕之進, 猪花美月, 冠ひかり, 和田涼香, 重吉沙衣, 住田卓也, 入江俊一, 池田健一, 泉津弘佑
    日本植物病理学会大会, Mar. 2024, Japanese
    Oral presentation

  • Transcriptomic profiling of high temperature-related genes during Pyricularia oryzae appressorium formation
    Sutthiphai Thanathip, 平岩紗幸, 中屋敷均, Jantasuriyarat Chatchawan, 池田健一
    日本植物病理学会大会, Mar. 2024, English
    Oral presentation

  • コムギいもち病菌汚染種子の生長過程における菌の局在性解析
    奥田樹, 内橋嘉一, 中屋敷均, 土佐幸雄, 池田健一
    日本植物病理学会関西部会, Sep. 2023, Japanese
    Oral presentation

  • いもち病菌の根への感染機構の解明（3）黒変病斑の特徴付けと黒変形成に関わるピリカラシンH合成遺伝子クラスターのPyricularia属菌における分布様式
    向子愷, 中屋敷均, 池田健一
    日本植物病理学会関西部会, Sep. 2023, Japanese
    Oral presentation

  • 感染環境を模した培養条件化におけるコムギいもち病菌の遺伝子発現解析
    森本熱海, 馬場智子, Thach An Dang, 小林奈月, 池田健一, 中屋敷均
    日本植物病理学会関西部会, Sep. 2023, Japanese
    Oral presentation

  • タマネギべと病菌とタマネギ由来DNAの土壌からの検出量の変化
    西村文宏, 池田健一, 片山貴博, 菰淵啓三
    日本植物病理学会関西部会, Sep. 2023, Japanese
    Oral presentation

  • おとり植物を利用したMirafiori lettuce big-vein virus発病リスクのモニタリング
    池田健一, 伊藤大貴, 中屋敷均
    日本植物病理学会関西部会, Sep. 2023, Japanese
    Oral presentation

  • 走査型電子顕微鏡を用いた糸状菌の菌糸形態の観察
    池田健一
    医学生物学電子顕微鏡技術学会大会, Sep. 2023, Japanese
    Oral presentation

  • 温度制御型グロースチャンバーを用いたイプコナゾール（IP）及びベノミル（Be）のコムギいもち病防除効果の評価
    内橋嘉一, 池田健一, 奥田樹, 足助聡一郎, 土佐幸雄
    日本植物病理学会大会, Mar. 2023, Japanese
    Oral presentation

  • A MoSET1-dependent Zn2/Cys6 transcription factor regulates appressorium formation through cation movement in Pyricularia oryzae
    Thach An Dang, Minh Ngoc Dang, Yusaku Tsukahara, Kenichi Ikeda, Hitoshi Nakayashiki
    日本植物病理学会大会, Mar. 2023, English
    Oral presentation

  • いもち病菌の根への感染機構の解明（２）メヒシバいもち病菌によるイネ根の生長抑制と黒変症状の要因解析
    向子愷, 鶴嶋鉄, 中屋敷均, 池田健一
    日本植物病理学会大会, Mar. 2023, Japanese
    Oral presentation

  • コムギ穂の登熟過程におけるいもち病菌の感染時期が種子汚染頻度に与える影響
    池田健一, 内橋嘉一, 奥田樹, 中屋敷均, 土佐幸雄
    日本植物病理学会大会, Mar. 2023, Japanese
    Oral presentation

  • イネ科いもち病菌ゲノムへ人為的に導入したDNA配列のヒストン修飾
    小林奈月, Thach An Dang, 池田健一, 中屋敷均
    日本植物病理学会関西部会, Sep. 2022, Japanese
    Oral presentation

  • 健全コムギ種子を用いたコムギいもち病菌感染種子の作出
    奥田樹, 内橋嘉一, 中屋敷均, 土佐幸雄, 池田健一
    日本植物病理学会関西部会, Sep. 2022, Japanese
    Oral presentation

  • 種子感染したコムギいもち病菌が生育過程において潜伏感染する可能性
    池田健一, 中屋敷均, 土佐幸雄
    日本植物病理学会関西部会, Sep. 2022, Japanese
    Oral presentation

  • タマネギべと病菌の土壌菌密度の変化
    西村文宏, 池田健一, 片山貴博, 菰淵啓三
    日本植物病理学会関西部会, Sep. 2022, Japanese
    Oral presentation

  • イネ根へのいもち病菌の感染において品種特異的抵抗性は機能しない
    向子愷, 中屋敷均, 池田健一
    日本植物病理学会大会, Mar. 2022, Japanese
    Oral presentation

  • 人工基質表面特性がいもち病菌の胞子発芽に与える影響
    柏木結, 吉村優, 森垣憲一, 中屋敷均, 池田健一
    日本植物病理学会大会, Mar. 2022, Japanese
    Oral presentation

  • イネ科植物いもち病菌における抑制的ヒストン修飾H3K9me3とH3K27me3による遺伝子発現制御
    小林奈月, Thach Dang, Kieu Pham, 池田健一, 中屋敷均
    日本植物病理学会大会, Mar. 2022, Japanese
    Oral presentation

  • いもち病菌のゲノム進化における遺伝子水平移行のインパクト
    小林奈月, Thach Dang, 池田健一, 中屋敷均
    日本植物病理学会大会, Mar. 2022, Japanese
    Oral presentation

  • リアルタイムPCRによる土壌からのタマネギべと病菌卵胞子のモニタリング
    西村文宏, 藤澤遥, 森充隆, 中島千晴, 川口真穂, 中西充, 岩本豊, 三室元気, 中村吉秀, 池田健一
    日本植物病理学会大会, Mar. 2022, Japanese
    Oral presentation

  • 緑色菌従属栄養植物カイサカネランの色素体構造
    山下由美, 高木大輔, 池田健一, 末次健司, 黒沢高秀, 遊川知久
    東北植物学会, Dec. 2021, Japanese
    Nominated symposium

  • 白紋羽病菌におけるウイルス伝搬能を獲得した変異株の細胞壁の特性評価
    畑中郁穂, 坂口耕太, 中屋敷均, 池田健一
    医学生物学電子顕微鏡技術学会 第37回学術講演会, Oct. 2021, Japanese
    Poster presentation

  • 植物－微生物間相互作用解析における電子顕微鏡技術
    池田健一
    第19回医学生物学電子顕微鏡シンポジウム, Oct. 2021, Japanese
    [Invited]
    Nominated symposium

  • 白紋羽病菌におけるウイルス伝搬能を獲得した変異株の細胞壁の特性評価
    畑中郁穂, 坂口耕太, 兼松聡子, 中屋敷均, 池田健一
    日本植物病理学会関西部会, Sep. 2021, Japanese
    Poster presentation

  • Alternaria brassicicolaのnit変異菌株の作製とその性状
    西村文宏, 森脇丈治, 池田健一, 森充隆
    日本植物病理学会関西部会, Sep. 2021, Japanese
    Poster presentation

  • コムギいもち病菌に対するベノミルの抑制効果
    内橋嘉一, 池田健一, 奥田樹, 川口藍乃, 松本純一, 足助聡一郎, 神頭武嗣, 土佐幸雄
    日本植物病理学会関西部会, Sep. 2021, Japanese
    Poster presentation

  • いもち病菌の病原性発現過程における糖代謝と脂質代謝のクロストーク
    楊鈞晧, 向子愷, 中屋敷均, 池田健一
    日本植物病理学会関西部会, Sep. 2021, Japanese
    Poster presentation

  • ヒストン修飾から見たイネ科植物いもち病菌のゲノム進化
    小林奈月, 池田健一, 中屋敷均
    日本植物病理学会関西部会, Sep. 2021, Japanese
    Poster presentation

  • いもち病菌の根への感染機構の解明（１）各種病原型菌株を接種したイネ科植物の反応特性
    向子愷, 岡田大樹, 中屋敷均, 池田健一
    日本植物病理学会関西部会, Sep. 2021, Japanese
    Poster presentation

  • コムギいもち病菌に対するペフラゾエート及びイプコナゾールの抑制効果
    内橋嘉一, 池田健一, 川口藍乃, 松本純一, 森田耕平, 足助聡一郎, 神頭武嗣, 土佐幸雄
    日本植物病理学会大会, Mar. 2021, Japanese
    Oral presentation

  • 白紋羽病菌におけるウイルス伝搬能を獲得した変異株の特性解析ー分生子形成に関する遺伝子発現変動ー
    坂口耕太, 畑中郁穂, 兼松聡子, 中屋敷均, 池田健一
    日本植物病理学会大会, Mar. 2021, Japanese

  • イネ科植物いもち病菌における抑制的ヒストン修飾H3K9me3とH3K27me3の相互作用
    小林奈月, Thi Minh Kieu Pham, 池田健一, 中屋敷均
    日本植物病理学会大会, Mar. 2021, Japanese
    Oral presentation

  • MoSet1-dependent Zn2/Cys6 transcription factor plays a role in physical signal-triggered appressorium formation
    An Thach Dang, Ngoc Minh Dang, Kenichi Ikeda, Hitoshi Nakayashiki
    日本植物病理学会大会, Mar. 2021, English
    Oral presentation

  • コムギいもち病菌における新規特異的検出用qPCR/LAMPプライマーの設計
    池田健一, 内橋嘉一, 奥田樹, 中屋敷均, 土佐幸雄
    日本植物病理学会大会, Mar. 2021, Japanese
    Oral presentation

  • Mirafiori lettuce big vein virusのLAMP法による検出
    伊藤大貴, 井上海, 岩本豊, 西口真嗣, 中屋敷均, 池田健一
    日本植物病理学会関西部会, Nov. 2020, Japanese

  • Functional characterization of MoSET1-dependent transcription factors in Pyricularia oryzae
    Thach An Dang, Yusaku Tsukahara, Minh Ngoc Dang, Kenichi Ikeda, Hitoshi Nakayashiki
    日本植物病理学会関西部会, Nov. 2020, Japanese

  • コムギいもち病菌における定量PCR法及びLAMP法による検出用プライマーの検討
    池田健一, 内橋嘉一, 川口藍乃, 中屋敷均, 土佐幸雄
    日本植物病理学会関西部会, Nov. 2020, Japanese
    Oral presentation

  • 白紋羽病菌におけるウイルス伝搬能を獲得した変異株の遺伝子発現解析
    坂口耕太, 畑中郁穂, 兼松聡子, 中屋敷均, 池田健一
    日本植物病理学会大会, Mar. 2020, Japanese
    Oral presentation

  • イネ科植物いもち病菌における各種クロマチン修飾の相互作用
    小林奈月, Kieu Pham, 池田健一, 中屋敷均
    日本植物病理学会大会, Mar. 2020, Japanese
    Oral presentation

  • イネ科植物いもち病菌のヒストンメチル基転移酵素MoSET1により制御されるリン酸化酵素群の機能解析
    濱根美穂子, 池田健一, 中屋敷均
    日本植物病理学会大会, Mar. 2020, Japanese
    Oral presentation

  • コムギいもち病菌のsmall rDNA-derived RNAs (srRNA)およびtRNA-derived small RNA (tsRNA)の特徴付け
    梅崎佑樹, Dang Ngoc Minh, Nguyen Quyet, 池田健一, 中屋敷均
    令和元年度日本植物病理学会関西部会, Sep. 2019, Japanese, Domestic conference
    Oral presentation

  • Building blocks法によるいもち病菌のTriacylglycerol lipase遺伝子群の機能解析
    池田健一, 前田健太郎, 中屋敷均
    令和元年度日本植物病理学会関西部会, Sep. 2019, Japanese, Domestic conference
    Oral presentation

  • 緑色菌従属栄養植物カイサカネランの色素体構造
    山下由美, 高木大輔, 池田健一, 末次健司, 黒沢高秀, 遊川知久
    日本植物学会第83回大会, Sep. 2019, Japanese, Domestic conference
    Oral presentation

  • Spatio-temporal regulation of morphological differentiation in Pyricularia oryzae.
    IKEDA KENICHI, 藤原 有紗, NAKAYASHIKI HITOSHI
    The 8th International Rice Blast Conference, May 2019, English, Chingdu, China, International conference
    Poster presentation

  • 澱粉生合成酵素SSI, IIIbの変異体を用いたイネ栄養器官における澱粉生合成機構の解析
    森田隆太郎, 三浦聡子, クロフツ尚子, 青木直大, 池田健一, 深山浩, 藤田直子
    第247回日本作物学会講演会, Mar. 2019, Japanese, Domestic conference

  • 輪紋病のシキミから見出された新規エマラウイルス様ウイルス
    下元 祥史, 岡田 知之, IKEDA KENICHI, 久保田 健嗣, 柳澤 広宣, 森田 泰彰
    平成３１年度日本植物病理学会大会, Mar. 2019, Japanese, 日本植物病理学会, つくば国際会議場, Domestic conference
    Oral presentation

  • コムギいもち病菌の生産する小分子RNAの体系的な特徴付け
    梅崎 佑樹, Quyet Nguyen, IKEDA KENICHI, NAKAYASHIKI HITOSHI
    平成31年度日本植物病理学会大会, Mar. 2019, Japanese, つくば国際会議場, Domestic conference
    Oral presentation

  • コムギいもち病菌Ourmia-like virusesの2種のサテライトRNA様因子
    李 唯衣, 大北 修平, IKEDA KENICHI, 鈴木 信弘, NAKAYASHIKI HITOSHI
    平成31年度日本植物病理学会大会, Mar. 2019, Japanese, つくば国際会議場, Domestic conference
    Oral presentation

  • Olpidium virulentusの休眠胞子におけるミラフィオリレタスビックベインウイルスの局在
    IKEDA KENICHI, 岩本 豊, 松浦 克成, 西口 真嗣, NAKAYASHIKI HITOSHI
    平成31年度日本植物病理学会大会, Mar. 2019, Japanese, つくば国際会議場, Domestic conference
    Oral presentation

  • ウリ科植物におけるPOPs輸送タンパク質の細胞内局在と分泌
    諏訪 瑞季, 岩渕 彩, IKEDA KENICHI, INUI HIDEYUKI
    日本農薬学会第36回農薬環境科学研究会, Nov. 2018, Japanese, 甲府、山梨県, Domestic conference
    Poster presentation

  • ヴァイロコントロールー菌類ウイルスを利用した病害防除法ー
    IKEDA KENICHI, 坂口 耕太, 伊東 祐弥, 兼松 聡子, NAKAYASHIKI HITOSHI
    日本共生生物学会 第２回大会, Nov. 2018, Japanese, 神戸大学, Domestic conference
    Poster presentation

  • いもち病菌の組織特異的感染戦略
    岡田 大樹, NAKAYASHIKI HITOSHI, IKEDA KENICHI
    日本共生生物学会 第２回大会, Nov. 2018, Japanese, 神戸大学, Domestic conference
    Poster presentation

  • 高知県のシシトウガラシから検出されたファイトプラズマ
    下元 祥史, IKEDA KENICHI, 森田 泰彰, 竹内 繁治
    平成30年度日本植物病理学会関西部会, Sep. 2018, Japanese, 山口大学, Domestic conference
    Oral presentation

  • ヒストンメチル基転移酵素MoSET1が制御するいもち病菌リン酸化酵素の機能解析
    濱根 美穂子, IKEDA KENICHI, NAKAYASHIKI HITOSHI
    平成30年度日本植物病理学会関西部会, Sep. 2018, Japanese, 山口大学, Domestic conference
    Oral presentation

  • いもち病菌のイネ根における宿主特異的寄生性評価
    岡田 大樹, NAKAYASHIKI HITOSHI, IKEDA KENICHI
    平成30年度日本植物病理学会関西部会, Sep. 2018, Japanese, 山口大学, Domestic conference
    Oral presentation

  • Dynamics of fungal cell structure during plant infection
    IKEDA KENICHI
    2nd e-ASIA Symposium on Functional Nano-Biology at Osaka University, Sep. 2018, English, Osaka University, International conference
    [Invited]
    Invited oral presentation

  • いもち病菌の脂質・糖代謝における付着器形成シグナル経路の関与．
    原田 叡人, 中本 知里, Ngyyen Hanh, NAKAYASHIKI HITOSHI, IKEDA KENICHI
    平成30年度日本植物病理学会大会, Mar. 2018, Japanese, 神戸国際会議場, Domestic conference
    Oral presentation

  • MoAgo3アルゴノートはMoAgo1とのsiRNAの競合によりRNAサイレンシングを負に制御する
    Nguyen Quyet, 松原 愛, IKEDA KENICHI, 鈴木 信弘, NAKAYASHIKI HITOSHI
    平成29年度 日本植物病理学会関西部会, Sep. 2017, Japanese, 大阪府立大学, Domestic conference
    Oral presentation

  • Localization of major latex-like proteins is important for contamination of Cucurbita pepo by persistant organic pollutants
    諏訪 瑞季, 岩渕 彩, 山崎 清志, IKEDA KENICHI, INUI HIDEYUKI
    The 14th International Symposium on Persistent Toxic Substances 2017, Sep. 2017, English, Nagoya, Japan, International conference
    Poster presentation

  • Building blocks法によるいもち病菌の脂質代謝遺伝子群の機能解析
    IKEDA KENICHI, 翠川 陽大, 前田 健太郎, NAKAYASHIKI HITOSHI
    平成29年度 日本植物病理学会関西部会, Sep. 2017, Japanese, 大阪府立大学, Domestic conference
    Oral presentation

  • Crucial steps for crop contamination by persistent organic pollutants in Cucurbitaceae family
    諏訪 瑞季, 岩渕 彩, IKEDA KENICHI, INUI HIDEYUKI
    DIOXIN 2017, Aug. 2017, English, Vancouver, Canada, International conference
    Poster presentation

  • 光合成装置の異常
    IKEDA KENICHI
    医学生物学電子顕微鏡技術学会, May 2017, Japanese, 神戸大学, Domestic conference
    [Invited]
    Nominated symposium

  • 土壌からのミラフィオリレタスビックベインウイルスのqRT-PCR法による検出
    井上 海, 松浦 克成, 岩本 豊, 西口 真嗣, IKEDA KENICHI, NAKAYASHIKI HITOSHI
    平成２９年度日本植物病理学会大会, Apr. 2017, Japanese, 盛岡, Domestic conference
    Oral presentation

  • Olpidium virulentusの遊走子放出に及ぼす土壌pHの影響
    IKEDA KENICHI, 井上 海, 岩本 豊, 相野 公孝, 松浦 克成, 前川 和正, NAKAYASHIKI HITOSHI, 西口 真嗣
    平成２９年度日本植物病理学会大会, Apr. 2017, Japanese, 盛岡, Domestic conference
    Oral presentation

  • 糸状菌におけるプログラム細胞死
    IKEDA KENICHI
    糸状菌分子生物学研究会 若手の会, Nov. 2016, Japanese, プラムイン城陽, Domestic conference
    [Invited]
    Invited oral presentation

  • Three argonaute protein and their associated sRNAs in Pyricularia oryzae
    Nguyen Q, Iritani S, Vu BV, Ohkita K, IKEDA KENICHI, NAKAYASHIKI HITOSHI
    7th International Rice Blast Conference, Oct. 2016, English, The Bellevue Manila, Philippines, International conference
    Oral presentation

  • Hydrophobin in a wheat isolate of Pyricularia oryzae: Mpg1, but not Mhp1, is essential for adhesion and pathogenicity
    IKEDA KENICHI, Kitaoka H, Inoue K, NAKAYASHIKI HITOSHI, PARK Pyoyun
    7th International Rice Blast Conference, Oct. 2016, English, The Bellevue Manila, Philippines, International conference
    Poster presentation

  • 白紋羽病菌におけるウイルス伝搬能を獲得した変異株の細胞学的解析
    伊東 祐弥, 兼松 聡子, NAKAYASHIKI HITOSHI, IKEDA KENICHI
    日本植物病理学会関西部会, Sep. 2016, Japanese, 静岡県コンベンションアーツセンター グランシップ, Domestic conference
    Oral presentation

  • コムギいもち病菌における3種の新規ourmiavirus様マイコウイルスの同定
    大北 修平, IKEDA KENICHI, 鈴木 信弘, NAKAYASHIKI HITOSHI
    日本植物病理学会関西部会, Sep. 2016, Japanese, 静岡県コンベンションアーツセンター グランシップ, Domestic conference
    Oral presentation

  • いもち病菌におけるオートファジー誘導機構の解明（２）オートファジーと付着器形成過程におけるシグナル経路の検証
    中本 知里, Nguyen Hieu Hanh, NAKAYASHIKI HITOSHI, IKEDA KENICHI
    日本植物病理学会関西部会, Sep. 2016, Japanese, 静岡県コンベンションアーツセンター グランシップ, Domestic conference
    Oral presentation

  • イネ科植物いもち病菌ゲノムに存在する3種のArgonauteにロードされるsRNAの比較解析
    入谷 彰英, Nguyen Quyet, 大北 修平, 横矢 佳奈, IKEDA KENICHI, NAKAYASHIKI HITOSHI
    日本植物病理学会関西部会, Sep. 2016, Japanese, 静岡県コンベンションアーツセンター グランシップ, Domestic conference
    Oral presentation

  • 糸状菌におけるウイルス伝搬能を獲得した変異株の特性解析
    伊東 祐弥, 森田 雄一, 上森 喬大, 井上 加奈子, NAKAYASHIKI HITOSHI, IKEDA KENICHI
    平成28年度植物感染生理談話会, Aug. 2016, Japanese, シーパル須磨（神戸）, Domestic conference
    Poster presentation

  • オートファジーと付着器形成過程におけるシグナル経路の解明
    中本 知里, 井上 加奈子, NAKAYASHIKI HITOSHI, IKEDA KENICHI
    平成28年度植物感染生理談話会, Aug. 2016, Japanese, シーパル須磨（神戸）, Domestic conference
    Poster presentation

  • ルテニウムレッドによる植物病原糸状菌の細胞外物質の染色効果
    IKEDA KENICHI, 上野 紳吾, PARK Pyoyun
    医学生物学電子顕微鏡技術学会, May 2016, Japanese, 日本大学医学部, Domestic conference
    Poster presentation

  • 植物病原菌バイオフィルム形成のin vitro評価系の確立
    IKEDA KENICHI, 森 友花, 曳地 康史
    近畿電顕技術情報交換会第38回談話会, Mar. 2016, Japanese, 神戸大学梅田インテリジェントラボラトリ, Domestic conference
    Oral presentation

  • いもち病菌におけるオートファジー誘導機構の解明（１）各種シグナル物質添加による影響評価
    中本 知里, NAKAYASHIKI HITOSHI, IKEDA KENICHI
    平成28年度日本植物病理学会大会, Mar. 2016, Japanese, 岡山コンベンションセンター, Domestic conference
    Oral presentation

  • Ralfuranones secreted by Ralstonia solanacearum function as intercellular secondary messengers during its biofilm formation
    森 友花, 大西 秀幸, 井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 木場 章範, 大西 浩平, 甲斐 建次, 曳地 康史
    第３０回 日本微生物生態学会, Oct. 2015, English, 亀城プラザ 土浦市, Domestic conference
    Poster presentation

  • ポリリン酸化合物処理による糸状菌病害防除法の開発
    大崎 裕介, 北川 博子, NAKAYASHIKI HITOSHI, IKEDA KENICHI
    平成２７年度日本植物病理学会関西部会, Sep. 2015, Japanese, あわぎんホール徳島県郷土文化会館, Domestic conference
    Oral presentation

  • Fungal pathogenicity -analysis example using electron microscopes-
    IKEDA KENICHI
    Plant Protection seminor, Sep. 2015, English, University of Philippines Los Banos, International conference
    [Invited]
    Invited oral presentation

  • 青枯病菌のＢＦ形成機構とその病原性への役割
    曳地 康史, 森 友花, 石川 詩歩, 東本 周樹, 井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 木場 章範, 大西 浩平, 甲斐 建次
    平成２７年度植物感染生理談話会, Aug. 2015, Japanese, 松山市, Domestic conference
    [Invited]
    Invited oral presentation

  • 糸状菌における不和合性反応を制御する遺伝子の解明
    伊東 祐弥, 森田 雄一, 上森 喬大, 井上 加奈子, NAKAYASHIKI HITOSHI, IKEDA KENICHI
    平成２７年度植物感染生理談話会, Aug. 2015, Japanese, 松山市, Domestic conference
    Poster presentation

  • いもち病菌の病原性発現過程におけるオートファジーの役割
    中本 知里, 井上 加奈子, NAKAYASHIKI HITOSHI, IKEDA KENICHI
    平成２７年度植物感染生理談話会, Aug. 2015, Japanese, 松山市, Domestic conference
    Poster presentation

  • いもち病菌ゲノムのエピジェネティックな遺伝子制御
    Pham Thi Minh Kieu, 井上 義博, Vu Van Ba, IKEDA KENICHI, NAKAYASHIKI HITOSHI
    平成２７年度植物感染生理談話会, Aug. 2015, Japanese, 松山市, Domestic conference
    [Invited]
    Invited oral presentation

  • Ralstonia solanacearum OE1-1株のバイオフィルム形成にレクチンRS-IILは必要である
    森 友花, 井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 大西 秀幸, 甲斐 建次, 木場 章範, 大西 浩平, 曳地 康史
    平成２７年度植物感染生理談話会, Aug. 2015, Japanese, 松山市, Domestic conference
    Poster presentation

  • 走査型電子顕微鏡を用いた代替電子染色剤の選抜法．
    IKEDA KENICHI, 嘉久 大毅, 井上 加奈子, PARK Pyoyun
    医学生物学電子顕微鏡技術学会, Jun. 2015, Japanese, 名古屋市立大学, Domestic conference
    Oral presentation

  • 明所高温条件で誘導されるステート２によるPSII障害回避機構の解析
    丸谷 曜子, YAMAUCHI YASUO, 東山 真理, AKIMOTO SEIJI, 井上 加奈子, IKEDA KENICHI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO
    日本植物生理学会, Mar. 2015, Japanese, 東京農業大学, Domestic conference
    Oral presentation

  • 青枯病菌のバイオフィルム形成への菌体外多糖の関与
    森 友花, 東本周樹, 井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 木場 章範, 大西 浩平, 曳地 康史
    日本植物病理学会大会, Mar. 2015, Japanese, 明治大学, Domestic conference
    Oral presentation

  • 次世代シーケンサを用いた白紋羽病菌の細胞質不和合性反応における発現変動遺伝子の解析
    森田雄一, 上森喬大, 井上加奈子, NAKAYASHIKI HITOSHI, 兼松聡子, IKEDA KENICHI
    日本植物病理学会大会, Mar. 2015, Japanese, 明治大学, Domestic conference
    Oral presentation

  • ウリ科植物における残留性有機汚染物質の地上部移行メカニズム．
    岩渕 彩, 後藤 純弥, 吉原 亮平, IKEDA KENICHI, 井上 加奈子, INUI HIDEYUKI
    日本農芸化学会大会, Mar. 2015, Japanese, 岡山大学, Domestic conference
    Oral presentation

  • いもち病菌の根部感染過程にオートファジーは必須ではない．
    IKEDA KENICHI, 井上加奈子, NAKAYASHIKI HITOSHI, Michael Kershaw, Nicholas Talbot
    日本植物病理学会大会, Mar. 2015, Japanese, 明治大学, Domestic conference
    Oral presentation

  • いもち病菌の根侵入において胞子内の核のS期への導入が重要である
    井上加奈子, 中屋敷 均, Miriam Oses-Ruiz, Wasin Salulkoo, Nicholas Talbot, IKEDA KENICHI
    日本植物病理学会大会, Mar. 2015, Japanese, 明治大学, Domestic conference
    Oral presentation

  • MAPキナーゼPmk1およびMps1はH3K4ヒストンメチル基転移酵素MoSET1の上流で機能する．
    グエン ハン, ファム キウ, IKEDA KENICHI, NAKAYASHIKI HITOSHI
    日本植物病理学会大会, Mar. 2015, Japanese, 明治大学, Domestic conference
    Oral presentation

  • Investigation the role of NADPH oxidases during infection related development of the rice blast fungus Magnaporthe oryzae.
    Lauren Ryder, IKEDA KENICHI, Magdelena Martin-Urdiroz, Michael Kershaw, Nicholas Talbot
    28th Fungal Genetics Conference, Mar. 2015, English, Pacific Grove, California, International conference
    Oral presentation

  • furanone合成酵素をコードするralA遺伝子は青枯病菌のバイオフィルム形成に関与する．
    森 友花, 大西秀幸, 井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 木場 章範, 大西 浩平, 曳地 康史
    日本植物病理学会大会, Mar. 2015, Japanese, 明治大学, Domestic conference
    Oral presentation

  • クモの寄生蜂による寄主クモの造網行動操作の起源と機能―ニールセンクモヒメバチを例に
    髙須賀 圭三, 安井 知己, ISHIGAMI TORU, 中田 兼介, IKEDA KENICHI, 松本 吏樹郎, MAETO KAORU
    第59回日本応用動物昆虫学会大会, 2015, Japanese, 山形大学(山形市), Domestic conference
    Oral presentation

  • Seed shattering behaviour and abscission layer formation in rice
    Cizuru Inoue, Than Myint Htun, Yumi Oka, Kanako Inoue, Ken-ichi Ikeda, Takashige Ishii, Ryo Ishikawa
    Marchantia Workshop 2014, Dec. 2014, English, Centennial Hall, Kobe University, International conference
    Poster presentation

  • The MAP kinase, Pmk1 is epistatic to MoSET1, H3K4 methyltransferase for infection-related morphogenesis in Magnaporthe oryzae
    Nguyen Hieu Hanh, Kieu Thi Minh Pham, Ikeda Kenichi, NAKAYASHIKI HITOSHI
    糸状菌分子生物学コンファレンス, Nov. 2014, Japanese, 仙台, Domestic conference
    Poster presentation

  • Toward plant disease protection -from the view of fungal genomics-
    IKEDA KENICHI
    Biosicence Seminar Series, Oct. 2014, English, University of Exeter, UK, International conference
    Public discourse

  • Toward plant disease protection -from the view of fungal genomics-
    IKEDA KENICHI
    Bioscience Seminar in University of Exeter, Oct. 2014, English, University of Exeter, United Kingdom, International conference
    Public discourse

  • 野生イネ(Oryza rufipogon)の遺伝的背景における種子脱粒性遺伝子座の相互作用と離層形態
    井上 千鶴, Than Myint Htun, INOUE KANAKO, IKEDA KENICHI, ISHII TAKASHIGE, ISHIKAWA RYO
    日本育種学会第126回講演会, Sep. 2014, Japanese, 日本育種学会, 南九州大学, Domestic conference
    Poster presentation

  • 白紋羽病菌の和合性及び不和合性反応についての細胞学的・分子生物学的解析
    森田 雄一, 上森 喬大, 井上加奈子, NAKAYASHIKI HITOSHI, 兼松聡子, IKEDA KENICHI
    日本植物病理学会関西部会, Sep. 2014, Japanese, 富山大学, Domestic conference
    Oral presentation

  • 光・温度条件の変化により形成されるPSI-LHCII超複合体の解析
    丸谷曜子, YAMAUCHI YASUO, 井上加奈子, IKEDA KENICHI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO
    日本農芸化学会関西支部会, Sep. 2014, Japanese, 奈良先端科学技術大学, Domestic conference
    Oral presentation

  • The MAP kinase, Pmk1 is epistatic to MoSET1, H3K4 methyltransferase for infection-related morphogenesis in Magnaporthe oryzae
    Nguyen Hieu Hanh, Kieu Thi Minh Pham, Ikeda Kenichi, NAKAYASHIKI HITOSHI
    日本植物病理学会関西部会, Sep. 2014, Japanese, Domestic conference
    Oral presentation

  • トマト植物の細胞間隙に接する細胞表面で青枯病菌はバイオフィルムを形成する
    森 友花, 井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, 尾上 孝利, 木場 章範, 大西 浩平, 曳地 康史
    日本植物病理学会全国大会, Jun. 2014, Japanese, 札幌コンベンションセンター, Domestic conference
    Oral presentation

  • Infection-related gene expression largely depends on MoSET1, the histone lysine methyltransferases catalyzing H3K4 methylation in Magnaporthe oryzae
    Kieu Pham Thi Minh, Yoshihiro Inoue, Ikeda Kenichi, NAKAYASHIKI HITOSHI
    日本植物病理学会, Jun. 2014, Japanese, 札幌, Domestic conference
    Oral presentation

  • MoSET1ヒストンメチルカトランスフェラーゼに依存したいもち病菌の感染器官形成時の遺伝子発現
    Pham Kieu, 井上 喜博, IKEDA KENICHI, NAKAYASHIKI HITOSHI
    日本植物病理学会全国大会, Jun. 2014, Japanese, Domestic conference
    Oral presentation

  • コムギの光化学系における高温耐性機構の解析：ステート遷移の誘導と超高速励起型PSI-LHCII複合体の形成
    丸谷 曜子, YAMAUCHI YASUO, AKIMOTO SEIJI, 井上 加奈子, IKEDA KENICHI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO
    第5回日本光合成学会年会, May 2014, Japanese, 近畿大学, Domestic conference
    Poster presentation

  • 高温ストレスを受けたコムギにおけるチラコイド膜の構造変化を伴う光化学系の再編成：熱放散とステート遷移の誘導
    丸谷 曜子, YAMAUCHI YASUO, AKIMOTO SEIJI, 井上 加奈子, IKEDA KENICHI, MIZUTANI MASAHARU, SUGIMOTO YUKIHIRO
    第55回日本植物生理学会年会, Mar. 2014, Japanese, 富山大学, Domestic conference
    Poster presentation

  • ウリ科植物の根における脂溶性物質結合タンパク質の局在性．
    吉原亮平, 後藤純弥, 岩淵彩, IKEDA KENICHI, 井上加奈子, INUI HIDEYUKI
    日本農芸化学会2014年度大会, Mar. 2014, Japanese, 明治大学, Domestic conference
    Oral presentation

  • The lecM of Ralstonia solanacearum is involved in its attachment to the intercellular spaces.
    Mori Y, Inoue K, IKEDA KENICHI, Kiba A, Ohnishi K, Hikichi Y
    The 87th Annual Meeting of Japanese Society for Bacteriology, Mar. 2014, English, Tower Hall Funahori, Tokyo, Domestic conference
    Oral presentation

  • いもち病菌におけるヒストンリジンメチルトランスフェラーゼの役割
    Pham Kieu, 井上 喜博, Vu Ba, Nguyen Quoc, IKEDA KENICHI, NAKAYASHIKI HITOSHI
    糸状菌分子生物学コンファレンス, Nov. 2013, Japanese, Domestic conference
    Oral presentation

  • Histone lysine methyltransferases in Magnaporthe oryzae are involved in various aspects of infection and pathogenesis.
    Pham, K. T. M, Vu, B. V, Nguyen, Q. B, IKEDA KENICHI, Nakayashiki Hitoshi
    6th International Rice Blast Conference 2013, Aug. 2013, English, International conference
    Oral presentation

  • 生物における活性酸素種の働き 植物病原菌が感染するしくみを探る
    森田 雄一, IKEDA KENICHI
    大阪市立大学 博士人材開発フォーラム, 2013, Japanese, Domestic conference
    [Invited]
    Invited oral presentation

  • 植物病原糸状菌感染時において単子葉植物と双子葉植物で誘導されるパピラの形成機序に関する細胞学的比較解析
    井上 加奈子, 宮田 奈緒, 毛呂 恵, NAKAYASHIKI HITOSHI, PARK Pyoyun, IKEDA KENICHI
    日本植物病理学会関西部会, 2013, Japanese, Domestic conference
    Poster presentation

  • 植物病原糸状菌の剥離作用による新規生物防除法
    IKEDA KENICHI
    第2回ネイチャー・インダストリー・アワード, 2013, Japanese, Domestic conference
    Poster presentation

  • 糸状菌の細胞質不和合性反応におけるプログラム細胞死機構の解析
    上森 喬大, 井上 加奈子, 北澤 大知, 木田 千晶, NAKAYASHIKI HITOSHI, 兼松 聡子, IKEDA KENICHI
    日本土壌微生物学会, 2013, Japanese, Domestic conference
    Poster presentation

  • 糸状菌の細胞質不和合性反応におけるプログラム細胞死機構の解析
    上森 喬大, 井上 加奈子, 木田 千晶, NAKAYASHIKI HITOSHI, 兼松 聡子, IKEDA KENICHI
    日本植物病理学会関西部会, 2013, Japanese, Domestic conference
    Poster presentation

  • 高等植物におけるalkenal/one oxidoreductase (AOR)の生理学的解析 植物も糖尿病を発症する
    齋藤 亮太, 伊福 健太郎, IKEDA KENICHI, 池田 加奈子, MIYAKE CHIKAHIRO
    日本土壌肥料学会関西支部会, 2013, Japanese, Domestic conference
    Oral presentation

  • リアルタイム定量PCR法による菌核病生物防除剤Coniothyrium minitansの特異的検出
    宿南 良, 岩本 豊, 小川 宗和, IKEDA KENICHI
    日本土壌微生物学会, 2013, Japanese, Domestic conference
    Poster presentation

  • リアルタイム定量PCR法による菌核病生物防除剤Coniothyrium minitansの追跡調査
    宿南 良, 岩本 豊, 小川 宗和, NAKAYASHIKI HITOSHI, IKEDA KENICHI
    日本植物病理学会関西部会, 2013, Japanese, Domestic conference
    Poster presentation

  • ヒストンメチル転移酵素群はイネ科植物いもち病菌の感染において重要な役割を果たす
    Pham Kieu, Vu Ba, Nguyen Quoc, IKEDA KENICHI, 井上 喜博, TOSA YUKIO, NAKAYASHIKI HITOSHI
    日本植物病理学会大会, 2013, Japanese, Domestic conference
    Oral presentation

  • ナシ黒斑病菌のRacAの病原性及び胞子発芽に及ぼす影響
    森田 雄一, 玄 康洙, 伊藤 理紗, NAKAYASHIKI HITOSHI, PARK Pyoyun, IKEDA KENICHI
    日本植物病理学会大会, 2013, Japanese, Domestic conference
    Oral presentation

  • いもち病菌の胞子発芽時におけるオートファジー誘導の経時的解析
    井上 加奈子, 坂井田 彩野, 兼松 聡子, NAKAYASHIKI HITOSHI, PARK Pyoyun, IKEDA KENICHI
    日本植物病理学会大会, 2013, Japanese, Domestic conference
    Oral presentation

  • Remodeling of photosystems to induce thermal dissipation and state transition accompanied by structural changes of thylakoid membranes in heat-stressed wheat
    Yoko Marutani, Yasuo Yamauchi, Akimoto S, Kanako Inoue, Ken-ichi Ikeda, Masaharu Mizutani, Yukihiro Sugimoto
    The 6th Asia and Oceania Conference on Photobiology, 2013, English, International conference
    Poster presentation

  • Multiple host adhesion factors in Magnaporthe oryzae –potential target for disease control-
    IKEDA KENICHI, Kitagawa H, Inoue K, Shimoi S, Osaki Y, Park Pyoyun, Nakayashiki Hitoshi
    6th International Rice Blast Conference 2013, 2013, English, International conference
    Poster presentation

  • MoSET1, histone methyltransferase responsible for methylation of histone H3 lysine 4 in Magnaporthe oryzae is required for both activation of gene expression during infection-related morphogenesis of the fungus
    Pham Kieu, Vu Ba, IKEDA KENICHI, NAKAYASHIKI HITOSHI
    日本植物病理学会関西部会, 2013, Japanese, Domestic conference
    Oral presentation

  • Macroautophagy during appressorium morphogenesis in Magnaporthe oryzae
    Inoue K, IKEDA KENICHI, Nakayashiki Hitoshi
    6th International Rice Blast Conference 2013, 2013, English, International conference
    Poster presentation

  • Histone lysine methyltransferases in Magnaporthe oryzae are involved in various aspects of infection and pathogenesis
    Pham Kieu, Vu Ba, Nguyen Quoc, IKEDA KENICHI, Nakayashiki Hitoshi
    10th International Congress of Plant Pathology, 2013, English, International conference
    [Invited]
    Invited oral presentation

  • Autophagy-inducing condition during infection process of Magnaporthe oryzae
    Inoue K, Nakayashiki Hitoshi, IKEDA KENICHI
    10th International Congress of Plant Pathology, 2013, English, International conference
    Poster presentation

  • Attachment of airborne pathogens to their host; a potential target for disease control
    IKEDA KENICHI, Kitagawa H, Shimoi S, Inoue K, Osaki Y, Nakayashiki Hitoshi, Park Pyoyun
    10th International Congress of Plant Pathology, 2013, English, International conference
    [Invited]
    Invited oral presentation

  • 病原菌を剥離させるアプローチによるイネいもち病生物防除法の開発
    IKEDA KENICHI
    日本植物病理学会 バイオコントロール研究会「生物農薬が直面している問題点と今後の展開」, 2012, Japanese, Domestic conference
    [Invited]
    Invited oral presentation

  • 病原菌を剥離させるアプローチによるイネいもち病生物防除法の開発
    IKEDA KENICHI
    バイオコントロール研究会, 2012, Japanese, 福岡市, Domestic conference
    [Invited]
    Invited oral presentation

  • 代替電子染色の現状
    IKEDA KENICHI
    第２５回電子顕微鏡技術研修会, 2012, Japanese, 鳥取大学, Domestic conference
    [Invited]
    Invited oral presentation

  • 代替電子染色の現状
    IKEDA KENICHI
    第25回電子顕微鏡技術研修会 夏の学校2012, 2012, Japanese, Domestic conference
    [Invited]
    Invited oral presentation

  • 糸状菌の細胞質不和合性反応における細胞死の機能解析
    木田 千晶, 井上 加奈子, PARK Pyoyun, IKEDA KENICHI
    日本土壌微生物学会, 2012, Japanese, Domestic conference
    Poster presentation

  • ナシ黒斑病菌の付着器局在性NADPH oxidase Bは侵略力発揮に必須である
    森田 雄一, 玄 康洙, NAKAYASHIKI HITOSHI, 稲田 のりこ, PARK Pyoyun, IKEDA KENICHI
    日本植物病理学会関西部会, 2012, Japanese, Domestic conference
    Oral presentation

  • ゼラチナーゼ活性を有する微生物の葉面定着性の向上
    北川 博子, PARK Pyoyun, IKEDA KENICHI
    日本植物病理学会大会, 2012, Japanese, Domestic conference
    Oral presentation

  • ゼラチナーゼ活性を有する土壌微生物を利用した植物病害防除法の開発
    北川 博子, 井上 加奈子, 下井 沙紀, IKEDA KENICHI, PARK Pyoyun
    日本土壌微生物学会, 2012, Japanese, Domestic conference
    Poster presentation

  • クリ胴枯病菌における菌類ウイルス構成タンパク質の免疫電顕観察
    井上 加奈子, IKEDA KENICHI, 鈴木 信弘, 兼松 聡子, PARK Pyoyun
    医学生物学電子顕微鏡技術学会, 2012, Japanese, Domestic conference
    Poster presentation

  • かびを病気にするウイルスで作物の病気を防ぐ
    IKEDA KENICHI
    日本土壌微生物学会市民公開講演会, 2012, Japanese, 神戸大学, Domestic conference
    [Invited]
    Invited oral presentation

  • かびを病気にするウイルスで作物の病気を防ぐ
    IKEDA KENICHI
    土壌微生物学会 市民公開講演会「土壌微生物を用いて作物の病気を防ぐ！」, 2012, Japanese, Domestic conference
    [Invited]
    Invited oral presentation

  • いもち病菌の根部感染過程における細胞構造の変化
    井上 加奈子, 村山 奈央子, PARK Pyoyun, IKEDA KENICHI
    日本植物病理学会大会, 2012, Japanese, Domestic conference
    Oral presentation

  • Virocontrol: transmission of hypovirulent mycoviruses to different fungal isolates using zinc compounds
    IKEDA KENICHI, Inoue K, Kitazawa H, Kida C, Uwamori T, Kanematsu S, Park P
    2nd Korea-Japan Joint Symposium for Plant Pathology, 2012, English, Fukuoka, Japan, International conference
    Poster presentation

  • Transmission of mycoviruses by attenuating programmed cell death in Rosellinia necatrix
    Inoue K, Kida C, Uwamori T, Kanematsu S, Park P, IKEDA KENICHI
    XV International Congress on Molecular Plant-Microbe Interac, 2012, English, Kyoto, Japan, International conference
    Poster presentation

  • Obstacle of "virocontrol" vacuole-mediated programmed cell death during heterogenic incompatibility in Rosellinia necatrix
    Uwamori T, Inoue K, Kida C, Kitazawa H, Kanematsu S, Park P, IKEDA KENICHI
    XV International Congress on Molecular Plant-Microbe Interac, 2012, English, Kyoto, Japan, International conference
    Poster presentation

  • Multiple host adhesion factors of extracellular matrix (ECM) in Magnaporthe oryzae -potential target for disease control
    Kitagawa H, Inoue K, Shimoi S, Kitaoka H, Park P, IKEDA KENICHI
    XV International Congress on Molecular Plant-Microbe Interac, 2012, English, Kyoto, Japan, International conference
    Poster presentation

  • Functional analyses of membrane binding NADPH oxidase complex, reactive oxygen species generation mechinery, in Alternaria alternata Japanese pear pathotype
    Morita Y, Hyon GS, Park P, IKEDA KENICHI
    2nd Korea-Japan Joint Symposium for Plant Pathology, 2012, English, Fukuoka, Japan, International conference
    Poster presentation

  • Detachment control: importance of ectracellular matrix (ECM) for pathogenicity and potential target for disease protection
    Kitagawa H, Inoue K, Shimoi S, Kitaoka H, Park P, IKEDA KENICHI
    2nd Korea-Japan Joint Symposium for Plant Pathology, 2012, English, Fukuoka, Japan, International conference
    Poster presentation

  • Cytological changes in the infection structure of Magnaporthe oryzae both on hydrophobic (leaf) and hydrophilic (root) surfaces
    Inoue K, Park P, IKEDA KENICHI
    2012 Korean Society of Plant Pathology International Conference, 2012, English, Seoul, Korea, International conference
    Poster presentation

  • Cellular dynamics of Magnaporthe oryzae during infection process both on hydrophobic (leaf) and hydrophilic (root) surface
    Inoue K, Park P, IKEDA KENICHI
    XV International Congress on Molecular Plant-Microbe Interac, 2012, English, Kyoto, Japan, International conference
    Poster presentation

  • Appressorium-localized NADPH oxidase is essential for aggresiveness and pathogenicity in Alternaria alternata
    IKEDA KENICHI, Morita Y, Hyon GS, Park P
    2012 Korean Society of Plant Pathology International Conference, 2012, English, Seoul, Korea, International conference
    [Invited]
    Invited oral presentation

  • Appressorium-localized NADPH oxidase B is essential for aggressiveness and pathogenicity in host specific toxin producing fungus Alternaria alternata Japanese pear pathotype
    IKEDA KENICHI, Morita Y, Hyon GS, Hosogi N, Morikawa K, Kusaka M, Nakayashiki H, Inada N, Tsuge T, Park P
    XV International Congress on Molecular Plant-Microbe Interac, 2012, English, Kyoto, Japan, International conference
    Poster presentation

  • Appressorium differentiation of Magnaporthe oryzae is regulated by metabolism of storage products accompanied with autophagy in conidia
    Inoue K, Meguro H, Kanematsu S, Park P, IKEDA KENICHI
    2nd Korea-Japan Joint Symposium for Plant Pathology, 2012, English, Fukuoka, Japan, International conference
    Poster presentation

  • 病原菌のアキレス腱をターゲットとした病害防除戦略
    IKEDA KENICHI
    近畿作物・育種研究会公開シンポジウム, 2011, Japanese, 神戸大学, Domestic conference
    [Invited]
    Invited oral presentation

  • 病原菌のアキレス腱をターゲットとした病害防除戦略
    IKEDA KENICHI
    近畿作物・育種研究会公開シンポジウム「明日のための農学研究～現場の問題とその解決に向けて～」, 2011, Japanese, Domestic conference
    [Invited]
    Invited oral presentation

  • 白紋羽病菌細胞質不和合性における亜鉛化合物処理による菌糸融合の促進と菌類ウイルスの菌株間移行
    IKEDA KENICHI, 井上 加奈子, 兼松 聡子, PARK Pyoyun
    日本植物病理学会関西部会, 2011, Japanese, Domestic conference
    Oral presentation

  • 白紋羽病菌の細胞質不和合性阻害による菌類ウイルスの菌株間移行
    IKEDA KENICHI, 井上 加奈子, 兼松 聡子, PARK Pyoyun
    日本植物病理学会大会, 2011, Japanese, Domestic conference
    Poster presentation

  • 白・紫紋羽病菌の液胞介在型不和合性細胞死といもち病菌のオートファジーの形態学的比較解析
    井上 加奈子, IKEDA KENICHI, 兼松 聡子, PARK Pyoyun
    日本植物病理学会大会, 2011, Japanese, Domestic conference
    Poster presentation

  • 酢酸ウランの染色機序
    PARK Pyoyun, 井上 加奈子, IKEDA KENICHI
    第１２回医学生物学電子顕微鏡シンポジウム, 2011, Japanese, Domestic conference
    [Invited]
    Invited oral presentation

  • 植物病原菌の感染機構解明とウラン代替染色剤の開発
    IKEDA KENICHI
    植物電子顕微鏡若手ワークショップ2011, 2011, Japanese, Domestic conference
    Oral presentation

  • 植物組織などにおける過ヨウ素酸処理による樹脂剥離効果の軽減
    PARK Pyoyun, 宮原 総子, 井上 加奈子, 下井 沙紀, IKEDA KENICHI
    医学生物学電子顕微鏡技術学会, 2011, Japanese, Domestic conference
    Poster presentation

  • 塩化ハフニウム
    堀内 喜高, IKEDA KENICHI, 井上 加奈子, 兼松 聡子, PARK Pyoyun
    第１２回医学生物学電子顕微鏡シンポジウム, 2011, Japanese, Domestic conference
    [Invited]
    Invited oral presentation

  • 塩化サマリウムによるウラン代替染色法
    IKEDA KENICHI, 井上 加奈子, 兼松 聡子, PARK Pyoyun
    医学生物学電子顕微鏡技術学会, 2011, Japanese, Domestic conference
    Oral presentation

  • ナシ黒斑病菌の付着器における活性酸素種生成の特性解析
    森田 雄一, 玄 康洙, IKEDA KENICHI, PARK Pyoyun
    医学生物学電子顕微鏡技術学会, 2011, Japanese, Domestic conference
    Poster presentation

  • ナシ黒斑病菌の感染器官における活性酸素種生成複合体の機能解析
    森田 雄一, 玄 康洙, 森川 響子, IKEDA KENICHI, PARK Pyoyun
    日本植物病理学会関西部会, 2011, Japanese, Domestic conference
    Oral presentation

  • ナシ黒斑病菌の感染器官における活性酸素種生成複合体の機能解析
    森田 雄一, 玄 康洙, 森川 響子, IKEDA KENICHI, PARK Pyoyun
    糸状菌分子生物学コンファレンス, 2011, Japanese, Domestic conference
    Poster presentation

  • いもち病菌の発芽過程における胞子の超微細構造観察
    井上 加奈子, IKEDA KENICHI, 兼松 聡子, PARK Pyoyun
    日本植物病理学会関西部会, 2011, Japanese, Domestic conference
    Oral presentation

  • Zinc compounds accelerate hyphal fusion and transmission of mycoviruses in Rosellinia necatrix
    IKEDA KENICHI, Inoue K, Kanematsu S, Park P
    XV International Congress of Virology, 2011, English, Sapporo, Japan, International conference
    [Invited]
    Invited oral presentation

  • Rosellinia necatrixとHelicobasidium mompaの液胞介在型不和合性細胞死とMagnaporthe oryzaeのオートファジーにおける形態学的比較解析
    井上 加奈子, IKEDA KENICHI, 兼松 聡子, PARK Pyoyun
    医学生物学電子顕微鏡技術学会, 2011, Japanese, Domestic conference
    Poster presentation

  • Magnaporthe oryzaeの分化様式とオートファジー機構による物質代謝の関連性
    井上 加奈子, 目黒 紘子, 兼松 聡子, PARK Pyoyun, IKEDA KENICHI
    糸状菌分子生物学コンファレンス, 2011, Japanese, Domestic conference
    Poster presentation

  • Heterogenic incompatibility and mycovirus transmission in Rosellinia necatrix
    IKEDA KENICHI, Inoue K, Kanematsu S, Park P
    2nd International Mycovirus Symposium, 2011, English, Otaru, Japan, International conference
    [Invited]
    Invited oral presentation

  • Cytological analysis on programmed cell death induced by mycelial incompatibility in Helicobasidium mompa and in Rosellinia necatrix
    Inoue K, Kanematsu S, Park P, IKEDA KENICHI
    XIII International Congress of Mycology, 2011, English, Sapporo, Japan, International conference
    Poster presentation

  • 白紋羽病菌の細胞質不和合性反応における細胞学的解析
    井上 加奈子, IKEDA KENICHI, 兼松 聡子, PARK Pyoyun
    日本植物病理学会大会, 2010, Japanese, Domestic conference
    Poster presentation

  • 単子葉・双子葉における植物病原菌感染に対するパピラ形成の細胞学的解析
    宮田 奈緒, 井上 加奈子, 玄 康洙, 森田 雄一, IKEDA KENICHI, PARK Pyoyun
    日本植物病理学会大会, 2010, Japanese, Domestic conference
    Poster presentation

  • 生物組織の超薄切片における酢酸ウランと塩化ハフニウムの電子染色性比較
    IKEDA KENICHI
    近畿電顕技術情報交換会第２９回談話会, 2010, Japanese, 堺市, Domestic conference
    [Invited]
    Invited oral presentation

  • 生物組織の超薄切片における酢酸ウランと塩化ハフニウムの電子染色性比較
    IKEDA KENICHI
    近畿電顕技術情報交換会, 2010, Japanese, Domestic conference
    [Invited]
    Invited oral presentation

  • 生物組織の超薄切片における酢酸ウランと塩化ハフニウムの電子染色性比較
    IKEDA KENICHI, 井上 加奈子, 堀内 喜高, 兼松 聡子, PARK Pyoyun
    医学生物学電子顕微鏡技術学会, 2010, Japanese, Domestic conference
    Poster presentation

  • 植物病原糸状菌における感染戦略の理解と防除の試み
    IKEDA KENICHI
    農薬学会第8回農薬バイオサイエンス研究会, 2010, Japanese, 京都大学, Domestic conference
    [Invited]
    Invited oral presentation

  • 磁性ナノビーズを用いた電気化学磁気シーディングによる動物用抗菌剤テトラサイクリンの選択分離
    工藤 幸会, 井原 一高, TOYODA KIYOHIKO, IKEDA KENICHI, 井上 加奈子, 梅津 一孝
    日本磁気科学会, 2010, Japanese, Domestic conference
    Oral presentation

  • 紫紋羽病菌の細胞質不和合性反応における経時的細胞観察
    井上 加奈子, IKEDA KENICHI, 兼松 聡子, PARK Pyoyun
    日本植物病理学会関西部会, 2010, Japanese, Domestic conference
    Poster presentation

  • 糸状菌の発芽胞子の微細構造観察のための固定法
    上野 紳吾, 井上 加奈子, 森田 雄一, 兼松 聡子, IKEDA KENICHI, PARK Pyoyun
    医学生物学電子顕微鏡技術学会, 2010, Japanese, Domestic conference
    Poster presentation

  • ハイドロフォビンMhp1のいもち病菌宿主接着に及ぼす影響
    北岡 治正, 井上 加奈子, NAKAYASHIKI HITOSHI, IKEDA KENICHI, PARK Pyoyun
    日本植物病理学会大会, 2010, Japanese, Domestic conference
    Poster presentation

  • ナシ黒斑病菌の付着器における活性酸素種生成の定量的解析
    森田 雄一, 玄 康洙, 艸香 將稔, IKEDA KENICHI, PARK Pyoyun
    日本植物病理学会大会, 2010, Japanese, Domestic conference
    Poster presentation

  • ナシ黒斑病菌の感染器官におけるRac1の機能解析
    森田 雄一, 玄 康洙, IKEDA KENICHI, PARK Pyoyun
    日本植物病理学会関西部会, 2010, Japanese, Domestic conference
    Poster presentation

  • ナシ黒斑病菌におけるNADPH oxidase遺伝子族の菌糸形態に及ぼす影響
    艸香 將稔, 玄 康洙, 森田 雄一, 村中 祥悟, IKEDA KENICHI, PARK Pyoyun
    日本植物病理学会大会, 2010, Japanese, Domestic conference
    Poster presentation

  • Rosellinia necatrixの細胞質不和合性反応における細胞像の経時的解析
    井上 加奈子, IKEDA KENICHI, 兼松 聡子, PARK Pyoyun
    医学生物学電子顕微鏡技術学会, 2010, Japanese, Domestic conference
    Poster presentation

  • Genetic analysis on mycelial incompatibility in Rosellinia necatrix
    IKEDA KENICHI, Inoue K, Nakamura H, Kanematsu S, Park P
    9th International Mycological Congress, 2010, English, Edinburgh, UK, International conference
    Poster presentation

  • Cytological analysis on mycelial incompatibility in Rosellinia necatrix
    Inoue K, IKEDA KENICHI, Kanematsu S, Park P
    9th International Mycological Congress, 2010, English, Edinburgh, UK, International conference
    Poster presentation

  • ウリ科植物ズッキーニにおける残留性有機汚染物質の吸収経路の解明
    山崎 清志, 前田 耕作, 細木 直樹, 玄 康洙, IKEDA KENICHI, PARK Pyoyun, IMAISHI HIROMASA, INUI HIDEYUKI
    第１８回環境化学討論会, Jun. 2009, Japanese, Domestic conference
    Oral presentation

  • ズッキーニ植物における残留性有機汚染物質の吸収・移行経路
    山崎 清志, 前田 耕作, 細木 直樹, 玄 康洙, IKEDA KENICHI, PARK Pyoyun, IMAISHI HIROMASA, INUI HIDEYUKI
    2009年度日本農芸化学会, Mar. 2009, Japanese, 福岡, Domestic conference
    Oral presentation

  • 白紋羽病菌の単子のう胞子後代における細胞質不和合性因子の解析．(I). 帯線形成に関わる因子の同定
    IKEDA KENICHI, 浜中 大輝, 大田 達郎, 中村 仁, 兼松 聡子, PARK Pyoyun
    日本植物病理学会大会, 2009, Japanese, Domestic conference
    Oral presentation

  • 白紋羽病菌の細胞質不和合性反応における二つの異なる細胞死
    井上 加奈子, IKEDA KENICHI, 中村 仁, 兼松 聡子, PARK Pyoyun
    日本植物病理学会関西部会, 2009, Japanese, Domestic conference
    Oral presentation

  • 新たないもち病防除法に向けたコラゲナーゼ活性を有する微生物のスクリーニング
    下井 沙紀, IKEDA KENICHI, YAMASAKI MASANORI, 對馬 誠也, PARK Pyoyun
    日本植物病理学会関西部会, 2009, Japanese, Domestic conference
    Poster presentation

  • 磁性ビーズを用いた電解磁気シーディングによるテトラサイクリン系抗生物質の連続分離
    工藤 幸会, IHARA IKKO, 北 幹子, TOYODA KIYOHIKO, IKEDA KENICHI, 井上 加奈子, 梅津 一孝
    日本磁気科学会年会, 2009, Japanese, Domestic conference
    Oral presentation

  • ナシ黒斑病菌のendo型ポリガラクツロナーゼは病原性に必須ではない
    藤本 卓, IKEDA KENICHI, 玄 康洙, 姜 山, PARK Pyoyun
    日本植物病理学会大会, 2009, Japanese, Domestic conference
    Poster presentation

  • ズッキーニ植物における残留農薬有機汚染物質の吸収・移行経路
    山崎 清志, 前田 耕作, 細木 直樹, 玄 康洙, IKEDA KENICHI, PARK Pyoyun, IMAISHI HIROMASA, INUI HIDEYUKI
    日本農芸化学会, 2009, Japanese, Domestic conference
    Oral presentation

  • ウリ科植物ズッキーニにおける残留性有機汚染物質の吸収経路の解明
    山崎 清志, 前田 耕作, 細木 直樹, 玄 康洙, IKEDA KENICHI, PARK Pyoyun, IMAISHI HIROMASA, INUI HIDEYUKI
    環境化学討論会, 2009, Japanese, Domestic conference
    Oral presentation

  • いもち病菌におけるハイドロフォビンの接着性と病原性に及ぼす影響
    井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, PARK Pyoyun
    日本植物病理学会大会, 2009, Japanese, Domestic conference
    Poster presentation

  • Water-involved resin blocks always give us bad thin-sections causing the poor cell images of biological tissues
    Meguro H, Inoue K, IKEDA KENICHI, Hyon GS, Hosogi N, Kanematsu S, Park Pyoyun
    6th International Symposium on Electron Microscopy in Medicine and Biology 2009, 2009, English, International conference
    Poster presentation

  • Ultrastructural analysis of the papilla formation during plant-microbe interaction
    Miyata N, IKEDA KENICHI, Suzuki T, Murayama N, Wada M, Park Pyoyun
    6th International Symposium on Electron Microscopy in Medicine and Biology 2009, 2009, English, International conference
    Poster presentation

  • Treatment of periodic acid may enhance the attachment ability between specimen surfaces and the ambient resin during ultrathin sectioning
    Shimoi S, IKEDA KENICHI, Kitaoka H, Inoue K, Hyon GS, Hosogi N, Kanematsu S, Park Pyoyun
    6th International Symposium on Electron Microscopy in Medicine and Biology 2009, 2009, English, International conference
    Poster presentation

  • The dynamics of reactive oxygen species generation during sclerotia development in Sclerotinia minor
    IKEDA KENICHI, Osato T, Inoue K, Hyon GS, Hosogi N, Kanematsu S, Park Pyoyun
    6th International Symposium on Electron Microscopy in Medicine and Biology 2009, 2009, English, International conference
    Poster presentation

  • Sclerotinia minorの菌核形成における活性酸素種の役割 (II)
    IKEDA KENICHI, 大里 友之, 井上 加奈子, 兼松 聡子, PARK Pyoyun
    日本植物病理学会関西部会, 2009, Japanese, Domestic conference
    Oral presentation

  • Pathological roles of reactive oxygen species in plant pathogen
    Hyon GS, Morita Y, Kusaka M, Hosogi N, IKEDA KENICHI, Nakayashiki Hitoshi, Park Pyoyun
    6th International Symposium on Electron Microscopy in Medicine and Biology 2009, 2009, English, International conference
    Oral presentation

  • Oxygen-bubbly glutaraldehyde fixation can preserve mucoproteins or mucopolysaccharides in the vacuoles of plant cells
    Kitaoka H, IKEDA KENICHI, Inoue K, Kanematsu S, Park Pyoyun
    6th International Symposium on Electron Microscopy in Medicine and Biology 2009, 2009, English, International conference
    Poster presentation

  • Mycelial incompatibility in Rosellinia necatrix
    Inoue K, IKEDA KENICHI, Kitazawa H, Hamanaka T, Ohta T, Kanematsu S, Park Pyoyun
    6th International Symposium on Electron Microscopy in Medicine and Biology 2009, 2009, English, International conference
    Poster presentation

  • Evaluation of the toxic effect of azoxystrobin on Botrytis cinerea
    Park Pyoyun, Tsurumi T, Inoue K, Hyon GS, Hosogi N, Ishii H, Kanematsu S, IKEDA KENICHI
    6th International Symposium on Electron Microscopy in Medicine and Biology 2009, 2009, English, International conference
    Poster presentation

  • A novel staining method of methanolic hafnium chloride (MHC) for uranyl acetate; staining effect of MHC for thin sections of bacteria, fungi, human biopsies, rat tissues and plants
    Horiuchi Y, Onoe T, Inoue K, IKEDA KENICHI, Park Pyoyun
    6th International Symposium on Electron Microscopy in Medicine and Biology 2009, 2009, English, International conference
    Poster presentation

  • A novel staining method for thin sections and en bloc tissues of rat kidney fixed with glutaraldehyde and osmium tetroxide using methanolic hafnium chloride
    Onoe T, Horiuchi Y, Inoue Kanako, IKEDA KENICHI, Park Pyoyun
    6th International Symposium on Electron Microscopy in Medicine and Biology 2009, 2009, English, International conference
    Oral presentation

  • 糖タンパク分解酵素によるいもち病菌の感染器官の剥離効果
    IKEDA KENICHI, 井上 加奈子, PARK Pyoyun
    第1回神戸大学バイオサイエンス研究会, 2008, Japanese, Domestic conference
    Poster presentation

  • 植物－植物病原糸状菌相互作用における活性酸素の役割
    IKEDA KENICHI, 朴杓允
    医学生物学電子顕微鏡技術学会第２４回学術講演会, 2008, Japanese, 横須賀市, Domestic conference
    [Invited]
    Invited oral presentation

  • 植物と病原菌のあいだの駆け引きと騙し合い
    IKEDA KENICHI
    神戸大学農学研究科公開講座生き物のつながりをゲノムで眺めたら？―インターゲノミクスで拓くこれからの農学―」, 2008, Japanese, Domestic conference
    [Invited]
    Invited oral presentation

  • 細胞像の読み取り
    朴杓允, IKEDA KENICHI, 金丸研吾
    医学生物学電子顕微鏡技術学会第２４回学術講演会, 2008, Japanese, 横須賀市, Domestic conference
    [Invited]
    Invited oral presentation

  • 各種糸状菌の胞子発芽過程における貯蔵物質の代謝変動
    目黒 紘子, 井上 加奈子, IKEDA KENICHI, PARK Pyoyun
    日本植物病理学会, 2008, Japanese, Domestic conference
    Oral presentation

  • 灰色かび病菌におけるストロビルリン系薬剤の発芽抑制効果
    鶴見 知洋, IKEDA KENICHI, PARK Pyoyun
    日本植物病理学会関西部会, 2008, Japanese, Domestic conference
    Oral presentation

  • ナシ黒斑病菌における活性酸素種 (reactive oxygen species: ROS)生成遺伝子noxAおよびnoxB変異株の細胞学・病理学的比較調査
    玄 康洙, 森田 雄一, IKEDA KENICHI, NAKAYASHIKI HITOSHI, PARK Pyoyun
    日本植物病理学会大会, 2008, Japanese, Domestic conference
    Oral presentation

  • いもち病菌の胞子発芽における天然栄養源の影響
    井上 加奈子, IKEDA KENICHI, PARK Pyoyun
    日本植物病理学会大会, 2008, Japanese, Domestic conference
    Oral presentation

  • いもち病菌におけるハイドロフォビン（Mpg1）の病原性への影響
    井上 加奈子, IKEDA KENICHI, PARK Pyoyun
    第1回神戸大学バイオサイエンス研究会, 2008, Japanese, Domestic conference
    Poster presentation

  • いもち病菌におけるハイドロフォビン(Mpg1)の接着能力への影響
    井上 加奈子, IKEDA KENICHI, NAKAYASHIKI HITOSHI, PARK Pyoyun
    第8回糸状菌分子生物学コンファレンス, 2008, Japanese, Domestic conference
    Poster presentation

  • イネ葉面フロラからのコラゲナーゼ活性を有する微生物のスクリーニング
    下井 沙紀, IKEDA KENICHI, YAMASAKI MASANORI, 對馬 誠也, PARK Pyoyun
    日本農芸化学会, 2008, Japanese, Domestic conference
    Oral presentation

  • Sclerotinia minorの菌核形成における活性酸素種の病理学的役割（2）活性酸素種の局在
    大里 友之, IKEDA KENICHI, PARK Pyoyun
    日本植物病理学会関西部会, 2008, Japanese, Domestic conference
    Oral presentation

  • Sclerotinia minorの菌核形成における活性酸素種の病理学的役割
    大里 友之, IKEDA KENICHI, PARK Pyoyun
    日本植物病理学会大会, 2008, Japanese, Domestic conference
    Oral presentation

  • Possible roles of extracellular matrix (ECM) in phytopathogenic fungus Magnaporthe oryzae
    IKEDA KENICHI, Inoue K, Park P
    9th Asia-Pacific Microscopy Conference, 2008, English, Jeju, Korea, International conference
    Poster presentation

  • Pathological roles of reactive oxygen species at penetration pegs of Alternaria alternata Japanese pear pathotype during plant infection
    Hyon GS, Morita Y, Hosogi N, IKEDA KENICHI, Nakayashiki H, Park P
    9th Asia-Pacific Microscopy Conference, 2008, English, Jeju, Korea, International conference
    [Invited]
    Invited oral presentation

  • Pathological roles of reactive oxygen species at infection structures of Alternaria alternata Japanese pear pathotype during plant infection
    玄 康洙, 森田 雄一, IKEDA KENICHI, NAKAYASHIKI HITOSHI, PARK Pyoyun
    第1回神戸大学バイオサイエンス研究会・若手研究者交流会, 2008, Japanese, Domestic conference
    Poster presentation

  • Dynamics of double-stranded RNA in white and violet root rot fungi
    IKEDA KENICHI, Nakamura H, Matsumoto N
    1st International Mycovirus Workshop in Japan, 2008, English, Kurashiki, Japan, International conference
    [Invited]
    Invited oral presentation

  • Cytological study for pathological roles of compounds analogous to AK-toxins, disease determinants, during plant infection
    Hosogi N, Hyon GS, Osato T, Inoue K, IKEDA KENICHI, Park P
    9th Asia-Pacific Microscopy Conference, 2008, English, Jeju, Korea, International conference
    [Invited]
    Invited oral presentation

  • 植物感染時に生じる活性酸素種の電顕定量法
    玄 康洙, 竹本 大吾, 田中 愛子, Bary Scott, 姜 山, IKEDA KENICHI, PARK Pyoyun
    医学生物学電子顕微鏡技術学会, 2007, Japanese, Domestic conference
    Oral presentation

  • ナシ黒斑病菌の貫穿菌糸における活性酸素種（ROS）生成遺伝子NoxBの機能解析
    玄 康洙, 森田 雄一, 篠木 武, IKEDA KENICHI, NAKAYASHIKI HITOSHI, PARK Pyoyun
    日本植物病理学会関西部会, 2007, Japanese, Domestic conference
    Oral presentation

  • ナシ黒斑病菌の貫穿菌糸における活性酸素種（ROS）生成遺伝子NoxAの機能解析
    玄 康洙, 篠木 武, IKEDA KENICHI, NAKAYASHIKI HITOSHI, PARK Pyoyun
    日本植物病理学会, 2007, Japanese, Domestic conference
    Oral presentation

  • トラップ法による白紋羽病菌マイコウイルスの探索
    古屋 典子, 井出 洋一, 中村 仁, IKEDA KENICHI, 松本 直幸
    日本植物病理学会, 2007, Japanese, Domestic conference
    Oral presentation

  • いもち病菌小型分生子の病理学的役割を解明するための基礎調査
    細木 直樹, CHUMA IZUMI, 篠木 武, IKEDA KENICHI, NAKAYASHIKI HITOSHI, MAYAMA SHIGEYUKI, TOSA YUKIO, PARK Pyoyun
    日本植物病理学会, 2007, Japanese, Domestic conference
    Oral presentation

  • いもち病菌感染器官の各種阻害剤処理による剥離効果
    井上 加奈子, IKEDA KENICHI, PARK Pyoyun
    日本植物病理学会, 2007, Japanese, Domestic conference
    Oral presentation

  • いもち病菌の胞子発芽における接着能力
    井上 加奈子, IKEDA KENICHI, PARK Pyoyun
    日本植物病理学会関西部会, 2007, Japanese, Domestic conference
    Oral presentation

  • いもち病菌のシトシンDNAメチル化は同菌の生活環において必須ではない
    IKEDA KENICHI, 田中 正起, 村田 聡樹, 椎名 宏太, TOSA YUKIO, MAYAMA SHIGEYUKI, PARK Pyoyun, NAKAYASHIKI HITOSHI
    日本植物病理学会関西部会, 2007, Japanese, Domestic conference
    Oral presentation

  • Possible roles of the extracellular matrix (ECM) from Magnaporthe oryzae during the fungus-host adhesion
    Inoue K, IKEDA KENICHI, Park P
    4th International Rice Blast Conference, 2007, English, Changsha, China, International conference
    Poster presentation

  • MoDim2, the Magnaporthe oryzae methyltransferase orthologous to Neurospora crassa Dim-2 is dispensable for the life cycle of the fungus in nature
    IKEDA KENICHI, Shiina K, Kadotani N, Tanaka M, Murata T, Chuma I, Tosa Y, Park P, Mayama S, Nakayashiki H
    4th International Rice Blast Conference, 2007, English, Changsha, China, International conference
    Poster presentation

  • Development of targeted evolution system of resistance genes LRR (leucine rich repeat) to recognize blast surface protein
    Kawasaki S, IKEDA KENICHI, Hirano K
    4th International Rice Blast Conference, 2007, English, Changsha, China, International conference
    [Invited]
    Invited oral presentation

  • 糖タンパク分解酵素によるコムギいもち病菌の感染器官の宿主粘着阻害に関する細胞学研究
    井上 加奈子, 姜 山, 細木 直樹, 玄 康洙, 兪 輝星, PARK Pyoyun, IKEDA KENICHI
    日本植物病理学会大会, 2006, Japanese, Domestic conference
    Oral presentation

  • ロイシンリッチリピートの進化工学的改変による任意分子の認識タンパク質作出法の確立
    IKEDA KENICHI, 平野 恒, 川崎 信二
    日本分子生物学会, 2006, Japanese, Domestic conference
    Poster presentation

  • ナシ黒斑病菌の貫穿菌糸における活性酸素種（ROS）の機能解析
    玄 康洙, IKEDA KENICHI, NAKAYASHIKI HITOSHI, PARK Pyoyun
    第6回糸状菌分子生物学コンファレンス, 2006, Japanese, Domestic conference
    Poster presentation

  • 捕捉法により土壌より分離された白紋羽病菌からの菌類ウイルス（二本鎖RNA）の検出
    IKEDA KENICHI, 中村 仁, 松本 直幸
    日本植物病理学会大会, 2005, Japanese, Domestic conference
    Oral presentation

  • いもち病防除戦略をゲノム進化から考える
    IKEDA KENICHI
    ゲノム情報を基盤としたイネ-いもち病菌相互作用の解析と耐病性機構の解明, 2005, Japanese, 東京大学, Domestic conference
    [Invited]
    Invited oral presentation

  • イネ科植物いもち病菌におけるレトロトランスポゾンMAGGYのメチル化遺伝子の同定
    椎名宏太, IKEDA KENICHI, 角谷直樹, NAKAYASHIKI HITOSHI, TOSA YUKIO, MAYAMA SHIGEYUKI
    日本植物病理学会関西部会, 2005, Japanese, Domestic conference
    Oral presentation

  • DNA methyltransferaseは転移因子由来siRNAの蓄積を促進する
    角谷 直樹, 椎名 宏太, IKEDA KENICHI, TOSA YUKIO, MAYAMA SHIGEYUKI, NAKAYASHIKI HITOSHI
    第7回RNA学会, 2005, Japanese, 弘前大学, Domestic conference
    Oral presentation

  • Some aspects on the life cycle of two Helicobasidium species in Japan
    Nakamura Hitoshi, IKEDA KENICHI, Akahira T, Matsumoto Naoyuki
    4th Asia-Pacific Mycological Congress and 9th International Marine and Freshwater Mycology Symposium, 2004, English, Chang Mai, International conference
    Oral presentation

■ Affiliated Academic Society
  • 関西病虫害研究会

  • バイオコントロール研究会

  • 糸状菌分子生物学研究会

  • 日本土壌微生物学会

  • 医学生物学電子顕微鏡技術学会

  • 日本植物病理学会

■ Research Themes
  • Functional analysis of NADPH oxidase 1 in filamentous fungi
    池田 健一
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, Apr. 2024 - Mar. 2027

  • Visualization of cell cycle in phytopathogenic fungus Pyricularia oryzae
    池田 健一
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Kobe University, Apr. 2020 - Mar. 2023
    これまでの成果より、いもち病菌胞子懸濁液を接種した環境条件の違いにより細胞分裂の制御が大きく異なることが明らかとされた。この環境条件の違いに着目して、市販のカバーガラスを用いて付着器形成評価を進めたところ、製造ロットにより付着器形成率が変動することが明らかとなった。そこで、安定して付着器を形成する条件を検討するために、基板修飾技術を用いてガラス表面に様々な特性を付与して、付着器形成の頻度を評価した。 その結果、これまで疎水性表面において付着器が形成されるとされてきたが、十分に洗浄処理した親水性表面においては、付着器が形成され、化学的修飾により親水性を付与した表面においては、付着器形成が阻害された。一方、化学的修飾により疎水性を付与した表面においては、根において感染する際に形成されることで知られる菌足様の構造を有する新たな発芽様式が観察された。この結果は従来考えられてきたいもち病菌における付着器形成誘導条件に関する新たな知見をもたらすものであった。 また、タマネギ表皮においていもち病菌胞子懸濁液を接種し、植物細胞への侵入行動を観察した。この際にヒストンを蛍光タンパク質RFPにて標識した変異株を用いることにより核の挙動を観察することができる。一般的には、付着器を形成し、成熟する過程において胞子に存在する全ての核はオートファジー機構により分解されてしまう。しかし、タマネギ表皮における感染の際には、胞子に存在する核の一部は残存していた。このことは、人工基質における付着器形成と宿主植物細胞への侵入における付着器形成においてオートファジー活性や細胞周期制御機構が異なっている可能性を示唆している。これら違いについて次年度は解析を進めて行く予定である。

  • 池田 健一
    学術研究助成基金助成金／挑戦的研究（萌芽）, Apr. 2017 - Mar. 2019, Principal investigator
    Competitive research funding

  • Evaluation of rice domestication by loss of seed shattering
    Ishikawa Ryo, IKEDA KEN-ICHI
    Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Kobe University, 01 Apr. 2014 - 31 Mar. 2017
    Loss of seed shattering was one of the key phenotypic changes selected for in the domestication of many crop species. Asian cultivated rice, Oryza sativa L., was domesticated from its wild ancestor, O. rufipogon, and three seed-shattering loci, qSH1, sh4 and qSH3, have been reported to be involved in the loss of seed shattering in cultivated rice. In this study, we analysed the seed-shattering behaviour of wild rice using introgression lines carrying the cultivated alleles from O. sativa Nipponbare in the genetic background of wild rice, O. rufipogon W630. We found that the interaction of mutations at these two loci may have played a role in the initial loss of seed shattering during rice domestication.

  • 朴 杓允
    学術研究助成基金助成金／基盤研究(C), Apr. 2013 - Mar. 2016
    Competitive research funding

  • 池田 健一
    科学研究費補助金／若手研究(A), 2011, Principal investigator
    Competitive research funding

  • 朴 杓允
    科学研究費補助金／基盤研究(C), 2010
    Competitive research funding

  • 朴 杓允
    科学研究費補助金／基盤研究(B), 2008
    Competitive research funding

  • 池田 健一
    科学研究費補助金／若手研究(B), 2007, Principal investigator
    Competitive research funding

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