Research activity information

• The effect of general anesthetics on neutrophil-like differentiated HL60 cells: sevoflurane activates the mitochondrial function to promote their bactericidal action.

  Kosuke Asei, Yuki Nomura, Daichi Fujimoto, Mayu Ooi, Norihiko Obata, Satoshi Mizobuchi

  BACKGROUND: Recent research has suggested that general anesthetics may affect the immune system, but it is unclear how they affect the mitochondria of neutrophils. The effects of general anesthetics on neutrophil-like differentiated HL60 cells mitochondrial function and their production of reactive oxygen species (ROS) were examined in this study. METHODS: HL60 cells were differentiated into neutrophil-like cells by treating them with 1 μM all-trans-retinoic acid for 4 days. The differentiated HL60 cells were exposed to propofol (4 μg ml-1), midazolam (0.5 μg ml-1), or sevoflurane (3 %) for 1 h or 5 h. Following the exposure to the anesthetics, we analyzed the production of ROS, bactericidal effects, cell viability, and apoptosis of the neutrophil-like differentiated HL60 cells. We also investigated the effects of these anesthetics on mitochondrial morphology and function of the neutrophil-like differentiated HL60 cells. RESULTS: Administration of propofol or midazolam activated neutrophil-like differentiated HL60 cells mitochondrial respiration and increased ATP production (P < 0.05). However, there was no significant change in production of ROS or cell death. Exposure to sevoflurane activated mitochondrial respiration, ATP production (P < 0.05) and ROS production (P < 0.05). Additionally, exposure to sevoflurane led to a significant increase in phagocytosis. Filamentous mitochondria appeared after treatment with propofol, midazolam, and sevoflurane, while fragmented and filamentous mitochondria were seen in untreated neutrophil-like differentiated HL60 cells. CONCLUSION: We investigated the effects of three anesthetics on the mitochondrial and bactericidal functions of differentiated HL60 cells. Our results indicate that sevoflurane activates mitochondrial function in differentiated HL60 cells, promotes the production of reactive oxygen species, and enhances bactericidal activity.

  Dec. 2025, Biochemistry and biophysics reports, 44, 102272 - 102272, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Lumbar Disc Herniation during Pregnancy: A Case Report and Literature Review.

  Yoshiyuki Takaishi, Hiroto Kajimoto, Hirotomo Tanaka, Masahiro Sugihara, Takeshi Kondoh, Mayu Ooi

  Low back pain during pregnancy is common. However, lumbar disc herniation in pregnancy is rare, and when severe, it can cause lower extremity pain that resists conservative treatment or lead to cauda equina syndrome. In this report, we describe a case of severe lower back pain that did not improve with conservative treatment, necessitating surgery at 12 weeks of gestation. Additionally, we reviewed the literature. A 35-year-old woman, 7 weeks pregnant, presented to our outpatient clinic with right lower extremity pain. Magnetic resonance imaging revealed a herniated disc at the right L4/5. Acetaminophen, a pregnancy-safe analgesic was prescribed. Despite initiating treatment, the pain persisted, and the patient was hospitalized for bed rest. After one month of conservative treatment with no relief, a repeat magnetic resonance imaging showed an enlarged herniated disc. After consulting with the patient and her family, surgery was performed at Kobe University Hospital, which offers comprehensive neurosurgery, obstetrics and gynecology, and anesthesiology services. At 12 weeks of gestation, the patient underwent a microscopic discectomy in the prone position under general anesthesia. Fluoroscopy for surgical level confirmation was minimized. The surgery lasted approximately 1 hour, and the right lower extremity pain was alleviated. Five months later, the patient delivered a healthy child. We report a case of surgery for a herniated disc during pregnancy, along with a review of the literature.

  2025, NMC case report journal, 12, 261 - 265, English, Domestic magazine

  Scientific journal


• The perioperative transition of serum biomarkers of a 1.5-year-old boy with very-long-chain acyl-CoA dehydrogenase deficiency.

  Ryosuke Bo, Hiroyuki Awano, Kenji Yamada, Mayu Ooi, Yuichi Okata, Yuko Bitoh, Satoshi Mizobuchi, Kazumoto Iijima

  Very long-chain acyl-coenzyme A dehydrogenase deficiency (VLCADD, OMIM 201475) is a congenital fatty acid oxidation disorder. Individuals with VLCADD should avoid catabolic states, including strenuous exercise and long-term fasting; however, such conditions are required when undergoing surgery. The perioperative management of VLCADD in infants has rarely been reported and details regarding the transition of serum biomarkers reflecting catabolic status have not been disclosed. Herein, we present the perioperative clinical and biological data of cryptorchidism in a 1.5-year-old boy with VLCADD. The patient was diagnosed through newborn screening and his clinical course was very stable. Genetic testing of ACADVL revealed compound heterozygous variants c.506 T > C (p.Met169Thr) and c.606-609delC (p.L216*). The enzyme activity of the patient with VLCAD was only 20% compared to that of healthy control. Left orchiopexy for the pediatric cryptorchidism was planned and performed at 1 and a half year of age. Induction anesthesia involved thiopental, fentanyl and rocuronium. The glucose infusion rate was maintained above 6.6 mg/kg/min starting the day before surgery until the operation was completed. Anesthesia was maintained with sevoflurane at approximately 2%. The serum concentration of tetradecenoylcarnitine were stable during the operation, ranging between 0.08 and 0.19 μM (cutoff <0.2 μM), and never deviated from the reference range. Concentration of other serum biomarkers including free fatty acid, 3-OH-butyrate, and creatine kinase, remained similarly unchanged. In this report, we describe the uneventful perioperative management of unilateral orchiopexy for left cryptorchidism in a 1.5-year-old boy with VLCADD using sufficient glucose infusion and volatile anesthesia.

  Jun. 2021, Molecular genetics and metabolism reports, 27, 100760 - 100760, English, International magazine

  Scientific journal


• Anesthetic management and associated complications of peroral endoscopic myotomy: A case series.

  Yuuki Nishihara, Takuya Yoshida, Mayu Ooi, Norihiko Obata, Shinichiro Izuta, Satoshi Mizobuchi

  AIM: To investigate the anesthetic management of peroral endoscopic myotomy (POEM) and its associated complications. METHODS: This study was a single-center, retrospective, observational study comprising a case series of all patients who underwent POEM in our hospital from April 2015 to November 2016. We collected data regarding patient characteristics, anesthetic methods, surgical factors, and complications using an electronic chart. RESULTS: There were 86 patients who underwent POEM in our hospital during the study period. Preoperatively, patients were maintained on a low residue diet for 48 h prior to the procedure. They were fasted of solids for 24 h before surgery. There was one case of aspiration (1.2%). During POEM, patients were positioned supine with the upper abdomen covered by a clear drape so that pneumoperitoneum could be timeously identified. In three cases, the peak airway pressure exceeded 35 cmH2O during volume controlled ventilation with tidal volumes of 6-8 mL/kg and subsequent impairment of ventilation. These cases had been diagnosed with spastic esophageal disorders (SEDs) and the length of the muscular incision on the esophageal side was longer than normal. CONCLUSION: In the anesthetic management of POEM, it is important to prevent aspiration during induction of anesthesia and to identify and treat complications associated with CO2 insufflation.

  Sep. 2018, World journal of gastrointestinal endoscopy, 10(9) (9), 193 - 199, English, International magazine

  Scientific journal


• Constant Current vs. Constant Voltage Systems for Temporal Spinal Cord Stimulation for Intractable Pain

  Mayu Ooi, Fujio Yanamoto, Hitoaki Sato, Yumiko Takao, Masako Okada, Moritoki Egi, Satoshi Mizobuchi

  Dec. 2017, ACTA MEDICA OKAYAMA, 71(6) (6), 531 - 537, English, Domestic magazine

  [Refereed]

  Scientific journal


• 日本と世界を-むすぶ-脊髄刺激療法を目指して

  Yanamoto Fujio, Ooi Mayu, Okada Masako, Takao Yumiko, Maekawa Nobuhiro

  Jun. 2012, 日本ペインクリニック学会誌, 19(3号) (3号), 285, Japanese

  [Refereed]

  Research society


• 痛み治療における馬尾神経刺激の有用性についてL2刺激は有効か?

  Okada Masako, Yanamoto Fujio, Ooi Mayu, Takao Yumiko, Maekawa Nobuhiro

  Jun. 2012, 日本ペインクリニック学会誌, 19(3号) (3号), 410, Japanese

  [Refereed]

  Research society


• 帯状疱疹後神経痛に対する脊髄神経根刺激療法の鎮痛効果

  Yanamoto Fujio, Okada Masako, Ooi Mayu, Takao Yumiko, Maekawa Nobuhiro

  Jun. 2012, 日本ペインクリニック学会誌, 19(3号) (3号), 412, Japanese

  [Refereed]

  Research society


• 脊髄刺激療法における定電流システムと定電圧システムの鎮痛効果の比較

  Ooi Mayu, Yanamoto Fujio, Okada Masako, Takao Yumiko, Maekawa Nobuhiro

  Jun. 2012, 日本ペインクリニック学会誌, 19(3号) (3号), 411, Japanese

  [Refereed]

  Research society


• 人工股関節置換術後の坐骨神経痛に対する疼痛管理を行った2症例

  Yanamoto Fujio, Ooi Mayu, Okada Masako, Takao Yumiko, Maekawa Nobuhiro

  Jun. 2012, 日本ペインクリニック学会誌, 19(3号) (3号), 342, Japanese

  Research society


• [Controversy in the treatment of acid-base abnormalities].

  Mayu Ooi, Nobuhiro Maekawa

  Sodium bicarbonate has been standard therapy for the treatment of acidosis. In lactic acidosis and hypercapnic acidosis, however, there is no clinical data supporting its effectiveness. We reviewed the literature of the efficacy of sodium bicarbonate on lactic acidosis and hypercapnic acidosis. On both conditions, we have no solid evidence supporting its beneficial effect. Conversely, acidosis or hypercapnia might be protective in acute lung and systemic organ injury. Therefore, the unprepared use of bicarbonate might be harmful in terms of fluid and sodium overload and excess lactate concentrations. According to current literature, we cannot recommend sodium bicarbonate administration for patients with lactic acidosis and hypercapnic acidosis.

  Mar. 2011, Masui. The Japanese journal of anesthesiology, 60(3) (3), 314 - 21, Japanese, Domestic magazine

  Scientific journal

• 小児の周術期における輸液療法

  大井 まゆ

  (一社)日本小児麻酔学会, Oct. 2023, 日本小児麻酔学会誌, 29(Suppl.) (Suppl.), 128 - 128, Japanese


• 小児周術期輸液管理について

  大井 まゆ

  (一社)日本小児麻酔学会, Oct. 2021, 日本小児麻酔学会大会プログラム, 26回, 22 - 22, Japanese


• 【実践-小児の輸液】各病態における輸液の考え方 外科疾患 手術中の輸液管理

  坪井 ちづ, 大井 まゆ

  (株)東京医学社, Apr. 2021, 小児内科, 53(4) (4), 655 - 658, Japanese


• 先天性サイトメガロウイルス感染に対して、胎児治療を施した2症例の麻酔経験

  松本 友里, 大井 まゆ, 野村 有紀, 岡田 雅子, 溝渕 知司

  (一社)日本小児麻酔学会, Oct. 2018, 日本小児麻酔学会誌, 24(Suppl.) (Suppl.), 182 - 182, Japanese


• 脊髄性筋萎縮症に対するヌシネルセン髄腔内投与における治療体制の構築

  横田 有理, 小幡 典彦, 野村 有紀, 大井 まゆ, 溝渕 知司

  (一社)日本小児麻酔学会, Oct. 2018, 日本小児麻酔学会誌, 24(Suppl.) (Suppl.), 188 - 188, Japanese


• 食道アカラシアに対する経口内視鏡的筋層切開術(POEM)の麻酔経験 48症例の検討

  西原 侑紀, 吉田 卓矢, 大井 まゆ, 小幡 典彦, 出田 眞一郎, 溝渕 知司

  日本臨床麻酔学会, Oct. 2016, 日本臨床麻酔学会誌, 36(6) (6), S275 - S275, Japanese


• 【震災後の痛み患者への対応:阪神淡路大震災と東日本大震災】 阪神淡路大震災における疼痛治療をふりかえって

  Yanamoto Fujio, Okada Masako, Ooi Mayu, Takao Yumiko, Maekawa Nobuhiro

  Apr. 2012, ペインクリニック, 33(4号) (4号), 486 - 494, Japanese

  [Invited]

  Introduction scientific journal

• 先天性サイトメガロウイルス感染に対して胎児治療を施した2症例の麻酔経験

  松本 友里, OOI MAYU, NOMURA YUKI, OKADA MASAKO, MIZOBUCHI SATOSHI

  日本小児麻酔学会第24回大会, Oct. 2018, Japanese, 神戸, Domestic conference

  Poster presentation


• 頭頚部癌患者における術後維持輸液の選択が術後血圧とカテコラミン投与時間に与える影響

  佐藤 千花子, EGI MORITOKI, 藤本 大地, OOI MAYU, OKADA MASAKO, MIZOBUCHI SATOSHI

  日本麻酔科学会第65回学術集会, May 2018, Japanese, 日本麻酔科学会, 横浜, Domestic conference

  Poster presentation


• The impact of intraoperative end tidal CO2 with the incidence of postoperative nausea and vomiting

  藤本 大地, Egi Moritoki, Nomura Yuki, Ooi Mayu, Obata Norihiko, Mizobuchi Satoshi

  日本麻酔科学会第64回学術集会, Jun. 2017, Japanese, 日本麻酔科学会, 神戸, Domestic conference

  Poster presentation


• 食道アカラシアに対する経口内視鏡的筋層切開術(POEM)の麻酔経験―48症例の検討―

  西原 侑紀, 吉田 卓矢, Ooi Mayu, Obata Norihiko, Izuta Shinichiro, Mizobuchi Satoshi

  The 36th Annual Meeteing of the Japan Society for Clinical Anesthesia, Nov. 2016, Japanese, The Japan Society for Clinical Anesthesia, 高知, Domestic conference

  Poster presentation


• anesthetic management of peroral endoscopic myotomy for achalasia with dilated esophagus

  吉田 卓矢, Izuta Shinichiro, Ooi Mayu, 岡田 卓也, Mizobuchi Satoshi

  第62回日本麻酔科学会関西支部学術集会, Sep. 2016, Japanese, 日本麻酔科学会関西支部, 大阪, Domestic conference

  Poster presentation


• The association of sodium concentration in postoperative intravenous fluid administration with the risk of postoperative hyponatremia

  横田 有理, Okada Masako, Ooi Mayu, Egi Moritoki, Sato Hitoaki, Mizobuchi Satoshi

  The 63rd Annual Meeting of the Japanese Society of Anesthesiologists, May 2016, Japanese, 日本麻酔科学会, 福岡, Domestic conference

  Poster presentation


• 当院における小児への頚静脈アプローチ中心静脈確保の検討

  吉田 絢香, Okada Masako, Morishita Jun, Ooi Mayu, Maekawa Nobuhiro

  第19回日本小児麻酔学会, Sep. 2013, Japanese, 日本小児麻酔学会, 神戸, 小児では術中の血管確保、経静脈栄養や化学療法を目的として全身麻酔下に中心静脈カテーテルが留置される。鎖骨下静脈確保は全例外科医が行っているが、頚静脈確保は従来外科医がカットダウンもしくは穿刺で行っていたのを2010年度より麻酔科医によるリアルタイム超音波ガイド下内頸静脈穿刺に移行してきた。移行前後での手技時間、合併症等について検討したので報告。, Domestic conference

  Poster presentation


• 新しい脊髄刺激療法に関する研究 日本人における臀部へのジェネレーター挿入

  Yanamoto Fujio, Takao Yumiko, 本山 泰士, Ueshima Eri, Okada Masako, Ooi Mayu, 佐藤 昭仁, 仁科 かほる, Maekawa Nobuhiro

  日本ペインクリニック学会 第47回大会, Jul. 2013, Japanese, 日本ペインクリニック学会, さいたま市, Domestic conference

  Poster presentation


• ラリンジアルマスクエアウェイとレミフェンタニルは相性が悪いのか？

  Kyohei Ueno, Jun Morishita, OOI MAYU, Nobuhiro Maekawa

  日本麻酔科学会第60回学術集会, May 2013, Japanese, 札幌, Domestic conference

  Poster presentation


• Can we perform cricoid pressure correctly?

  丸山 祐子, Morishita Jun, Ooi Mayu, Maekawa Nobuhiro

  The 59th Annual Meeting of the Japanese Society of Anesthesiologists, May 2012, Japanese, Japanese Society of Anesthesiologists, 神戸, Accuracy of cricoid pressure, Domestic conference

  Poster presentation


• 慢性疼痛患者に対するプレガバリンとガバペンチンの効果

  Takao Yumiko, Yanamoto Fujio, Sanada Kanae, Ooi Mayu, 坂本 昇太郎, Maekawa Nobuhiro

  第45回日本ペインクリニック学会, Jul. 2011, Japanese, 日本ペインクリニック学会, 松山, Domestic conference

  Poster presentation


• Isoflurane exposure of pregnant mice causes neurodegeneration and synaptogenesis in the developing brain of neonatal mice

  Ueshima Eri, 植木 正明, Ooi Mayu, Morishita Jun, 魚川 礼子, Maekawa Nobuhiro

  日本麻酔科学会第58回学術総会, May 2011, Japanese, 日本麻酔科学会, 神戸, Domestic conference

  Oral presentation


• 2MAC-Isoflurane exposure of pregnant mice causes nerodegeneration and synaptogenesis in the developing brain of neonatal mice

  魚川 礼子, 植木 正明, Morishita Jun, Ueshima Eri, Ooi Mayu, Maekawa Nobuhiro

  日本麻酔科学会第58回学術総会, May 2011, Japanese, 日本麻酔科学会, 神戸, Domestic conference

  Oral presentation


• 脊椎疾患の痛みちりょう脊椎疾患に対する薬物療法

  Yanamoto Fujio, Takao Yumiko, 坂本 昇太郎, Ooi Mayu, Sanada Kanae, Maekawa Nobuhiro, 村川 和重

  第41回日本ペインクリニック学会関西支部学術集会, May 2011, Japanese, 日本ペインクリニック学会関西支部, 大阪, Domestic conference

  Public symposium


• 成年女性に認めた坐骨神経痛の治療に難渋した2症例

  Yanamoto Fujio, Takao Yumiko, 坂本 昇太郎, Ooi Mayu, Sanada Kanae, Maekawa Nobuhiro

  第41回日本ペインクリニック学会関西支部学術集会, May 2011, Japanese, 日本ペインクリニック学会関西支部, 大阪, Domestic conference

  Oral presentation


• Environmental enrichment reverses learning impairment in the radial maze after neonatal exposure to isoflurane in mice

  植木 正明, Morishita Jun, Ueshima Eri, 魚川 礼子, Ooi Mayu, Maekawa Nobuhiro

  日本麻酔科学会第58回学術総会, May 2011, Japanese, 日本麻酔科学会, 神戸, Domestic conference

  Oral presentation


• 卵アレルギーを合併する児の筋生検の麻酔症例の検討

  魚川 玲子, Ooi Mayu, 渡海 裕文, Morishita Jun, 仁科 かほる, Maekawa Nobuhiro

  日本小児麻酔学会第16回学術集会, Sep. 2010, Japanese, 日本小児麻酔学会, 倉敷, Domestic conference

  Poster presentation

• 小児がんに対する麻酔薬の選択が予後および免疫機能へ与える影響

  大井 まゆ

  日本学術振興会, 科学研究費助成事業, 若手研究, 神戸大学, 01 Apr. 2020 - 31 Mar. 2023

  本研究は、血液悪性腫瘍と診断され、全身麻酔を要する小児患者において、使用する全身麻酔薬として吸入麻酔薬と静脈麻酔薬を比較し、化学 療法効果率、再発率、骨髄幹細胞生存率(Cell viability), 末梢血中の好中球・単球・リンパ球数および白血球における割合、血中INF-γ濃度、TGF-β濃度、 IL-4濃度の推移を検討することで、麻酔薬の選択が小児悪性血液腫瘍患者における予後および免疫機能へ与える影響を明らかにすることを目的とした無作為化比較試験である。 当該年度には対象患者の抽出前に、過去に研究対象の条件を満たした症例を後方視的に検討した。過去の手術及び麻酔件数から研究対象患者の人数、術後の血球数など通常の診療の範囲内で測定される数値の推移につき情報を収集した。血液腫瘍患者で全身麻酔を受ける患者の数は研究で検討できると考えられた。また、過去の研究報告などから統計学的に必要とされる症例数を割り出した。これまでの小児血液腫瘍患者では、麻酔法の選択は担当麻酔医へ任されていたが、吸入麻酔薬、静脈麻酔薬どちらも使用されておりその比はやや吸入麻酔薬が多かった。 そのため、本研究にて吸入麻酔薬、静脈麻酔薬を使用する症例を同数ずつ割り付けても、これまで提供してきた麻酔とそれほど大きな隔たりはないものと考える。 現在院内での倫理委員会への申請準備を行っており、承認を得られ次第、順次対象患者の抽出、無作為化、割り付けへと進めていく予定である。


• 画期的な気管挿管器具の開発を目指した研究

  大井 まゆ

  学術研究助成基金助成金／挑戦的萌芽研究, Apr. 2016 - Mar. 2018, Principal investigator

  Competitive research funding