Research activity information

• Apr. 2023 藤森科学研究財団, 研究助成

  Prihardi Kahar


• Mar. 2023 The Society of Chemical Engineers, Japan, 25th SCEJ Excellent award, Malic acid assimilation by yeast cells with surface display of esterase

  Saki Maehashi, Prihardi Kahar, Chiaki Ogino


• Nov. 2021 Young Asian Biological Engineer’s community (YABEC), Metabolic engineering of Saccharomyces cerevisiae BTCC3 for rapid gypsum-free lactic acid fermentation

  Radityo Pangestu, Prihardi Kahar, Chiaki Ogino


• Oct. 2016 Kobe University, Faculty of Engineering, Department of Chemical Science and Engineering, Certificate of Appreciation from the Dean of the Department of Chemical Science and Engineering

  Prihardi Kahar


• 2004 International Symposium on Biological Polyesters (ISBP), The Prominent Poster Award, Effective Production and Kinetic Characteristic of Ultra-high-molecular-weight of PHB in Recombinant Escherichia coli

  Prihardi Kahar

  International society


• Apr. 1998 公益財団法人橋谷奨学金

  Prihardi Kahar


• Apr. 1997 Sato Yo International Scholarship Foundation, Satoh Foundation Fellowship

  Prihardi Kahar


• Apr. 1992 Ministry of Research and Technology of Indonesia, Fellow of STAID (Science Technology for Industrial Development), Ministry of Research and Technology of Indonesia

  Prihardi Kahar

• Selective Interaction and Radio-Sensitizing Effect of Rutin-G-Modified Titanium Peroxide Nanoparticles on Breast Cancer Cells

  Winda Tasia, Koki Kawamoto, Kenta Morita, Yutaro Mori, Prihardi Kahar, Ryohei Sasaki, Chiaki Ogino

  Apr. 2025


• Factors Affecting D-Lactic Acid Production by Flocculant Saccharomyces cerevisiae Under Non-Neutralizing Conditions

  Dianti Rahmasari, Prihardi Kahar, Arthur Vinícius de Oliveira, Filemon Jalu Nusantara Putra, Akihiko Kondo, Chiaki Ogino

  Mar. 2025, Microorganisms

  [Refereed][Invited]

  Scientific journal


• Catalase-Knockout Complements the Radio-Sensitization Effect of Titanium Peroxide Nanoparticles on Pancreatic Cancer Cells

  Winda Tasia, Amane Washio, Koki Yamate, Kenta Morita, Yutaro Mori, Prihardi Kahar, Ryohei Sasaki, Chiaki Ogino

  Jan. 2025, Molecules

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Lipid Production from Palm Acid Oil (PAO) as a Sole Carbon Source by Meyerozyma guilliermondii

  Noor-Afiqah Ahmad Zain, Kar Ling Tan, Prihardi Kahar, Chiaki Ogino

  Corresponding, Jan. 2025, Processes

  [Refereed][Invited]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Lipid Production in Streptomyces jeddahensis Is Enhanced by Glucose and Fatty Acid Derivatives, with Temperature Variations Influencing Gene Expression and Biosynthesis

  Pamella Apriliana, Prihardi Kahar, Nova Rachmadona, Witta Kartika Restu, Akihiko Kondo, Chiaki Ogino

  Corresponding, Jan. 2025, Fermentation

  [Refereed]

  Scientific journal


• Evaluation of lipid production efficiency using palm oil mill effluent as a carbon source by Lipomyces starkeyi

  Nova Rachmadona, Prihardi Kahar, Ario Betha Juanssilfero, Fajriana Shafira Nurrusyda, Dewa Ayu Shintya Laura Arista Dewi, Irwan Kurnia, Iman Rahayu, Chiaki Ogino

  Oct. 2024, Biomass Conversion and Biorefinery

  [Refereed]

  Scientific journal


• Adaptive laboratory evolution of Lipomyces starkeyi for high production of lignin derivative alcohol and lipids with comparative untargeted metabolomics-based analysis.

  Filemon Jalu Nusantara Putra, Prihardi Kahar, Akihiko Kondo, Chiaki Ogino

  BACKGROUND: Adaptive laboratory evolution (ALE) is an impactful technique for cultivating microorganisms to adapt to specific environmental circumstances or substrates through iterative growth and selection. This study utilized an adaptive laboratory evolution method on Lipomyces starkeyi for high tolerance in producing lignin derivative alcohols and lipids from syringaldehyde. Afterward, untargeted metabolomics analysis was employed to find the key metabolites that play important roles in the better performance of evolved strains compared to the wild type. Lignin, a prominent constituent of plant biomass, is a favorable source material for the manufacture of biofuel and lipids. Nevertheless, the effective transformation of chemicals produced from lignin into products with high economic worth continues to be a difficult task. RESULTS: In this study, we exposed L. starkeyi to a series of flask passaging experiments while applying selective pressure to facilitate its adaptation to syringaldehyde, a specific type of lignin monomeric aldehyde. Using ALE, we successfully developed a new strain, DALE-22, which can synthesize syringyl alcohol up to 18.74 mM from 22.28 mM syringaldehyde with 41.9% lipid accumulation. In addition, a comprehensive examination of untargeted metabolomics identified six specific crucial metabolites linked to the improved tolerance of the evolved strain in the utilization of syringaldehyde, including 2-aminobutyric acid, allantoin, 4-hydroxyphenethyl alcohol, 2-aminoethanol, tryptophan, and 5-aminovaleric acid. CONCLUSION: The results of our study reveal how L. starkeyi adapts to using substrates produced from lignin. These findings offer important information for developing strategies to improve the process of converting lignin into valuable products for sustainable biorefinery applications.

  Oct. 2024, Microbial cell factories, 23(1) (1), 270 - 270, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Insights in Synthetic Bioengineering and Aspects of Creating a New Biotechnology

  Prihardi Kahar

  Oct. 2024, SynBio

  [Refereed][Invited]

  Scientific journal


• Editing Streptomyces genome using target AID system fused with UGI‐degradation tag

  Pamella Apriliana, Prihardi Kahar, Norimasa Kashiwagi, Akihiko Kondo, Chiaki Ogino

  Aug. 2024, Engineering in Life Sciences

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Production of lignin monomeric alcohols and lipids from oil palm empty fruit bunch by a combination of alkaline nitrobenzene depolymerization and Lipomyces starkeyi bioconversion

  Filemon Jalu Nusantara Putra, Prihardi Kahar, Akihiko Kondo, Chiaki Ogino

  Aug. 2024, Biochemical Engineering Journal

  [Refereed]

  Scientific journal


• Complete sequence randomness of lactate-based copolymers, LAHBs with varied lactate monomer fractions employing a series of propionyl-CoA transferases.

  Sangho Koh, Ryota Endo, Prihardi Kahar, Yutaro Mori, Chiaki Ogino, Shinji Tanaka, Shinji Tanaka, Yusuke Imai, Seiichi Taguchi

  Previously, we synthesized an evolved version of a bio-based polylactide (PLA) on microbial platforms using our engineered lactate-polymerizing enzyme (LPE). This lactate (LA)-based copolyester, LAHB, has advantages over PLA, including improved flexibility and biodegradability, and its properties can be regulated through the LA fraction. To expand the LA-incorporation capacity and improve polymer properties, in the state of in vivo LAHB production, propionyl-CoA transferases (PCTs) that exhibited enhanced production of LA-CoA than the conventional PCTs were selected. Here, the present study has demonstrated that the LA fraction of LAHB could be altered using various PCTs. Enhanced PCT performance was achieved by balancing polymer production and cell growth. Both events are governed by the use of acetyl-CoA, a commonly shared key metabolite. This could be attributed to the different reactivities of individual PCTs towards acetyl-CoA, which serves both as a CoA donor and a leading compound in the TCA cycle. Interestingly, we found complete sequence randomness in the LAHB copolymers, independent of the LA fraction. The mechanism of LA fraction-independent sequence randomness is discussed. This new PCT-based strategy synergistically combines with the evolution of LPE to advance the LAHB project, and enables us to perform advanced applications other than LAHB production utilizing CoA-linked substrates.

  Jun. 2024, International journal of biological macromolecules, 133055 - 133055, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Production of single cell oil by Lipomyces starkeyi from waste plant oil generated by the palm oil mill industry.

  Noor-Afiqah Ahmad Zain, Prihardi Kahar, Kumar Sudesh, Chiaki Ogino, Akihiko Kondo

  Only a few reports available about the assimilation of hydrophobic or oil-based feedstock as carbon sources by Lipomyces starkeyi. In this study, the ability of L. starkeyi to efficiently utilize free fatty acids (FFAs) and real biomass like palm acid oil (PAO) as well as crude palm kernel oil (CPKO) for growth and lipid production was investigated. PAO, CPKO, and FFAs were evaluated as sole carbon sources or in the mixed medium containing glucose. L. starkeyi was able to grow on the medium supplemented with PAO and FFAs, which contained long-chain length FAs and accumulated lipids up to 35% (w/w) of its dry cell weight. The highest lipid content and lipid concentration were achieved at 50% (w/w) and 10.1 g/L, respectively, when L. starkeyi was cultured in nitrogen-limited mineral medium (-NMM) supplemented with PAO emulsion. Hydrophobic substrate like PAO could be served as promising carbon source for L. starkeyi.

  May 2024, Journal of bioscience and bioengineering, 138(2) (2), 153 - 162, English, Domestic magazine

  [Refereed]

  Scientific journal


• Comparative responses of flocculating and nonflocculating yeasts to cell density and chemical stress in lactic acid fermentation

  Radityo Pangestu, Prihardi Kahar, Chiaki Ogino, Akihiko Kondo

  Lead, Apr. 2024, Yeast

  [Refereed]

  Scientific journal


• Engineering robust yeast for neutralizing-agent-free production of lactic acid from sugarcane bagasse hydrolysate

  Radityo Pangestu, Prihardi Kahar, Lutfi Kholida, Urip Perwitasari, Ahmad Thontowi, Fahrurrozi Fahrurrozi, Puspita Lisdiyanti, Yopi Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  Lead, Jan. 2024

  [Refereed]


• Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered Corynebacterium glutamicum

  Taghreed Elkasaby, Dao Duy Hanh, Prihardi Kahar, Hideo Kawaguchi, Takashi Sazuka, Akihiko Kondo, Chiaki Ogino

  Itaconic acid is a promising biochemical building block that can be used in polymer synthesis. Itaconic acid is currently produced in industry by the natural producer fungus Aspergillus terreus using glucose as a main carbon source. Most research for itaconic acid production using lignocellulosic-based carbon sources was carried out by A. terreus. Engineered Corynebacterium glutamicum strain which can grow in presence of fermentation inhibitors without effect on growth, was used for production of itaconic acid using sweet sorghum juice and bagasse sugar lysate (BSL). BSL contains many inhibitors unlike sorghum juice. C. glutamicum could grow in the media containing both types of lignocellulose-based carbon sources without showing any growth inhibition, however, sorghum juice was better in itaconic acid production than BSL. Different constructed strains of C. glutamicum were used for itaconic acid production, however, C. glutamicum ATCC 13032 pCH-Tad1optAdi1opt strain expressing Adi1/Tad1 genes (trans-pathway) from Ustilago maydis proved to be better in itaconic acid production giving final titer of 8.4 and 4.02 g/L using sweet sorghum juice and BSL as the sole carbon sources by fed-batch fermentation. Our study is the first for production of itaconic acid using sweet sorghum juice and BSL. The present study also proved that C. glutamicum can be used for enhancing itaconic acid production using lignocellulosic-based carbon sources.

  Jan. 2024, Enzyme and Microbial Technology, 172, 110345 - 110345, English, International magazine

  [Refereed]

  Scientific journal


• Saccharification of Pretreated Empty Bunch by Concentrated Xylanase and Assisted by Cellulase

  Hans Wijaya, Prihardi Kahar, Kengo Sasaki, Nanik Rahmani, Euis Hermiati, Yopi, Chiaki Ogino, Bambang Prasetya, Rumella Simarmata, Margaretta Christita, Akihiko Kondo

  The effect of the membrane separation process to concentrate the xylanase was tested to restrict cellulase dosage on the lignocellulosic waste. Xylanase produced from Streptomyces lividans expressing an endo-xylanase gene from Kitasatospora sp. was concentrated by nanofiltration membrane NTR-7410. This resulted in a seven-fold increase in xylanase activity without changing its specific activity. The saccharification of dilute acid-pretreated empty fruit bunch (EFB) was performed by applying membrane-concentrated xylanase compared with its un-concentrated to commercial cellulase loaded at one filter paper unit/g-biomass. Both glucose and xylose yields obtained by using concentrated xylanase (19.49% and 41.51%, respectively) were higher compared to un-concentrated xylanase (11.71% and 28.71%, respectively). Xylose-assimilating Saccharomyces cerevisiae yeast at initial of 0.5 g/L was then inoculated into the sugar solutions. The yeast had increased ethanol titer and productivity from sugars obtained by using membrane-concentrated xylanase (7.13±0.60 g/L and 0.59±0.06 g/L/h, respectively), compared by using un-concentrated xylanase (4.56±0.43 g/L and 0.38±0.02 g/L/h, respectively).

  Dec. 2023, AIP Conference Proceedings, 2972(1) (1)

  [Refereed]

  International conference proceedings


• The bioconversion of lignin derivative aldehydes into high-value aromatic alcohols and lipids via Lipomyces starkeyi

  Filemon Jalu Nusantara Putra, Prihardi Kahar, Akihiko Kondo, Chiaki Ogino

  Lead, Nov. 2023, Biochemical Engineering Journal

  [Refereed]

  Scientific journal


• Flocculation influences the response to increased cell density and chemical stresses during lactic acid fermentation in yeast

  Chiaki Ogino, Radityo Pangestu, Prihardi Kahar, Akihiko Kondo

  Jul. 2023


• Sucrose Signaling Contributes to the Maintenance of Vascular Cambium by Inhibiting Cell Differentiation.

  Aoi Narutaki, Prihardi Kahar, Shunji Shimadzu, Shota Maeda, Tomoyuki Furuya, Kimitsune Ishizaki, Hidehiro Fukaki, Chiaki Ogino, Yuki Kondo

  Plants produce sugars by photosynthesis and use them for growth and development. Sugars are transported from source-to-sink organs via the phloem in the vasculature. It is well known that vascular development is precisely controlled by plant hormones and peptide hormones. However, the role of sugars in the regulation of vascular development is poorly understood. In this study, we examined the effects of sugars on vascular cell differentiation using a vascular cell induction system named Vascular cell Induction culture System Using Arabidopsis Leaves (VISUAL). We found that sucrose has the strongest inhibitory effect on xylem differentiation among several types of sugars. Transcriptome analysis revealed that sucrose suppresses xylem and phloem differentiation from cambial cells. Physiological and genetic analysis suggested that sucrose might function through the BES1 transcription factor, which is the central regulator of vascular cell differentiation. Conditional overexpression of cytosolic invertase led to a decrease in the number of cambium layers due to an imbalance between cell division and differentiation. Taken together, our results suggest that sucrose potentially acts as a signal that integrates environmental conditions with the developmental program.

  May 2023, Plant & cell physiology, English, Domestic magazine

  [Refereed]

  Scientific journal


• Pretreatment With Dilute Maleic Acid Enhances the Enzymatic Digestibility of Sugarcane Bagasse and Oil Palm Empty Fruit Bunch Fiber.

  Lucky Risanto, Deddy Triyono Nugroho Adi, Triyani Fajriutami, Hiroshi Teramura, Widya Fatriasari, Euis Hermiati, Prihardi Kahar, Akihiko Kondo, Chiaki Ogino

  Lignocellulose is resistant to degradation and requires pretreatment before hydrolytic enzymes can release fermentable sugars. Sulfuric acid has been widely used for biomass pretreatment, but high amount of degradation products usually occurred when using this method. To enhance accessibility to cellulose, we studied the performances of several dilute organic acid pretreatments of sugarcane bagasse and oil palm empty fruit bunch fiber. The results revealed that pretreatment with maleic acid yields the highest xylose and glucose release among other organic acids. The effects of concentration, duration of heating and heating temperature were further studied. Dilute maleic acid 1% (w/w) pretreatment at 180 °C was the key to its viability as a substitute for sulfuric acid. Moreover, maleic acid did not seem to highly promote the formation of either furfural or 5-HMF in the liquid hydrolysate after pretreatment.

  Nov. 2022, Bioresource technology, 369, 128382 - 128382, English, International magazine

  [Refereed][Invited]

  Scientific journal


• Valorization of Lignin and Its Derivatives Using Yeast

  Filemon Jalu Nusantara Putra, Prihardi Kahar, Akihiko Kondo, Chiaki Ogino

  As the third most plentiful biopolymer after other lignocellulosic derivates such as cellulose and hemicellulose, lignin carries abundant potential as a substitute for petroleum-based products. However, the efficient, practical, value-added product valorization of lignin remains quite challenging. Although several studies have reviewed the valorization of lignin by microorganisms, this present review covers recent studies on the valorization of lignin by employing yeast to obtain products such as single-cell oils (SCOs), enzymes, and other chemical compounds. The use of yeasts has been found to be suitable for the biological conversion of lignin and might provide new insights for future research to develop a yeast strain for lignin to produce other valuable chemical compounds.

  MDPI, Oct. 2022, PROCESSES, 10(10) (10), English

  [Refereed][Invited]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization.

  Radityo Pangestu, Prihardi Kahar, Lutfi Nia Kholida, Urip Perwitasari, Ahmad Thontowi, Fahrurrozi, Puspita Lisdiyanti, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  Acidic and chemical inhibitor stresses undermine efficient lactic acid bioproduction from lignocellulosic feedstock. Requisite coping treatments, such as detoxification and neutralizing agent supplementation, can be eliminated if a strong microbial host is employed in the process. Here, we exploited an originally robust yeast, Saccharomyces cerevisiae BTCC3, as a production platform for lactic acid. This wild-type strain exhibited a rapid cell growth in the presence of various chemical inhibitors compared to laboratory and industrial strains, namely BY4741 and Ethanol-red. Pathway engineering was performed on the strain by introducing an exogenous LDH gene after disrupting the PDC1 and PDC5 genes. Facilitated by this engineered strain, high cell density cultivation could generate lactic acid with productivity at 4.80 and 3.68 g L-1 h-1 under semi-neutralized and non-neutralized conditions, respectively. Those values were relatively higher compared to other studies. Cultivation using real lignocellulosic hydrolysate was conducted to assess the performance of this engineered strain. Non-neutralized fermentation using non-detoxified hydrolysate from sugarcane bagasse as a medium could produce lactic acid at 1.69 g L-1 h-1, which was competitive to the results from other reports that still included detoxification and neutralization steps in their experiments. This strategy could make the overall lactic acid bioproduction process simpler, greener, and more cost-efficient.

  Lead, Aug. 2022, Scientific reports, 12(1) (1), 13645 - 13645, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• The flocculant Saccharomyces cerevisiae strain gains robustness via alteration of the cell wall hydrophobicity.

  Prihardi Kahar, Akiho Itomi, Hikari Tsuboi, Miki Ishizaki, Misa Yasuda, Chie Kihira, Hiromi Otsuka, Nurlina Binti Azmi, Hana Matsumoto, Chiaki Ogino, Akihiko Kondo

  When lignocellulosic biomass is utilized as a fermentative substrate to produce biochemicals, the existence of a yeast strain resistant to inhibitory chemical compounds (ICCs) released from the biomass becomes critical. To achieve the purpose, in this study, Saccharomyces yeast strains from a NBRC yeast culture collection were used for exploration and evaluated in two different media containing ICCs that mimic one another but resemble the hydrolysate of real biomass. Among them, S. cerevisiae F118 strain shows robustness upon the fermentation with unique flocculation trait that was strongly responsive to ICC stress. When this strain was cultured in the presence of ICCs, its cell wall hydrophobicity increased dramatically, and reduced significantly when the ICCs were depleted, demonstrating that cell-surface hydrophobicity can also act as an adaptive response to the ICCs. Cells from the strain with the highest cell-wall hydrophobicity displayed progressively stronger flocculation, indicating that the F118 strain is having unique robustness under ICC stress. Gene expression perturbation analysis revealed that mot3 gene encoding regulatory Mot3p from the F118 strain was expressed in response to the concentration of ICCs. This gene was found to control expression of ygp1 gene that encoding Ygp1p, one of cell wall proteins. Deep sequencing analysis revealed that the Mot3p of the F118 strain features a unique insertion and deletion of nucleotides that encode glutamine or asparagine residues, particularly in N-terminal domain, as determined by comparison to the Mot3p sequence from the S288c strain, which was employed as a control strain. Furthermore, the cell wall hydrophobicity of the S288c strain was greatly enhanced and became ICC-responsive after gene swapping with the mot3 gene from the F118 strain. The gene-swapped S288c strain fermented 6-fold faster than the wild-type strain, producing 14.5 g/L of ethanol from 30 g/L of glucose consumed within 24 h in a medium containing the ICCs. These such modifications to Mot3p in unique locations in its sequence have a potential to change the expression of a gene involved in cell wall hydrophobicity and boosted the flocculation response to ICC stress, allowing for the acquisition of extraordinary robustness.

  Lead, Mar. 2022, Metabolic engineering, 72, 82 - 96, English, International magazine

  [Refereed]

  Scientific journal


• Manno-Oligosaccharide Production from Biomass Hydrolysis by Using Endo-1,4-beta-Mannanase (ManNj6-379) from Nonomuraea jabiensis ID06-379

  Shanti Ratnakomala, Prihardi Kahar, Norimasa Kashiwagi, JaeMin Lee, Motonori Kudou, Hana Matsumoto, Pamella Apriliana, Yopi Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

  A novel endo-beta-1,4-mannanase gene was cloned from a novel actinomycetes, Nonomuraea jabiensis ID06-379, isolated from soil, overexpressed as an extracellular protein (47.8 kDa) in Streptomyces lividans 1326. This new endo-1,4-beta-mannanase gene (manNj6-379) is encoded by 445-amino acids. The ManNj6-379 consists of a 28-residue signal peptide and a carbohydrate-binding module of family 2 belonging to the glycoside hydrolase (GH) family 5, with 59-77% identity to GH5 mannan endo-1,4-beta-mannanase. The recombinant ManNj6-379 displayed an optimal pH of 6.5 with pH stability ranging between 5.5 and 7.0 and was stable for 120 min at 50 & DEG;C and lower temperatures. The optimal temperature for activity was 70 & DEG;C. An enzymatic hydrolysis assay revealed that ManNj6-379 could hydrolyze commercial beta-mannan and biomass containing mannan.

  Corresponding, MDPI, Feb. 2022, PROCESSES, 10(2) (2), 269 - 269, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• An integrated biorefinery strategy for the utilization of palm-oil wastes.

  Prihardi Kahar, Nova Rachmadona, Radityo Pangestu, Rendi Palar, Deddy Triyono Nugroho Adi, Ario Betha Juanssilfero, Yopi, Immanuel Manurung, Shinji Hama, Chiaki Ogino

  Each year, the palm oil industry generates a significant amount of biomass residue and effluent waste; both have been identified as significant sources of greenhouse gas (GHG) emissions. This issue poses a severe environmental challenge for the industry due to the possibility of long-term negative effects on human well-being. The palm-oil industry must invest significantly in the technology that is required to resolve these issues and to increase the industry's sustainability. However, current technologies for converting wastes such as lignocellulosic components and effluents into biochemical products are insufficient for optimal utilization. This review discusses the geographical availability of palm-oil biomass, its current utilization routes, and then recommends the development of technology for converting palm-oil biomass into value-added products through an integrated biorefinery strategy. Additionally, this review summarizes the palm oil industry's contribution to achieving sustainable development goals (SDGs) through a circular bioeconomy concept.

  Lead, Nov. 2021, Bioresource technology, 344(PB) (PB), 126266 - 126266, English, International magazine

  [Refereed]


• Recent advances in lignocellulosic biomass white biotechnology for bioplastics.

  Hideo Kawaguchi, Kenji Takada, Taghreed Elkasaby, Radityo Pangestu, Masakazu Toyoshima, Prihardi Kahar, Chiaki Ogino, Tatsuo Kaneko, Akihiko Kondo

  Lignocellulosic biomass has great potential as an inedible feedstock for bioplastic synthesis, although its use is still limited compared to current edible feedstocks of glucose and starch. This review focuses on recent advances in the production of biopolymers and biomonomers from lignocellulosic feedstocks with downstream processing and chemical polymer syntheses. In microbial production, four routes composed of existing poly (lactic acid) and polyhydroxyalkanoates (PHAs) and the emerging biomonomers of itaconic acid and aromatic compounds were presented to review present challenges and future perspectives, focusing on the use of lignocellulosic feedstocks. Recently, advances in purification technologies decreased the number of processes and their environmental burden. Additionally, the unique structures and high-performance of emerging lignocellulose-based bioplastics have expanded the possibilities for the use of bioplastics. The sequence of processes provides insight into the emerging technologies that are needed for the practical use of bioplastics made from lignocellulosic biomass.

  Oct. 2021, Bioresource technology, 344(PB) (PB), 126165 - 126165, English, International magazine

  [Refereed]


• High enzymatic recovery and purification of xylooligosaccharides from empty fruit bunch via nanofiltration

  Hans Wijaya, Kengo Sasaki, Prihardi Kahar, Nanik Rahmani, Euis Hermiati, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  Lead, May 2020, Processes, 8(5) (5), 619, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Concentration of Lipase from Aspergillus oryzae Expressing Fusarium heterosporum by Nanofiltration to Enhance Transesterification

  Hans Wijaya, Kengo Sasaki, Prihardi Kahar, Emmanuel Quayson, Nova Rachmadona, Jerome Amoah, Shinji Hama, Chiaki Ogino, Akihiko Kondo

  Lead, {MDPI} {AG}, Apr. 2020, Processes, 8(4) (4), 450 - 450, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• High cell density cultivation of Lipomyces starkeyi for achieving highly efficient lipid production from sugar under low C/N ratio

  Rezky Lastinov Amza, Prihardi Kahar, Ario Betha Juanssilfero, Nao Miyamoto, Hiromi Otsuka, Chie Kihira, Chiaki Ogino, Akihiko Kondo

  Lead, Sep. 2019, Biochemical Engineering Journal, 149(15) (15), 107236, English

  [Refereed]


• Bioenergy and Biorefinery: feedstock, biotechnological conversion and products

  Jerome Amoah, Prihardi Kahar, Chiaki Ogino, Akihiko Kondo

  Biorefinery has been suggested to provide relevant substitutes to a number of fossil products. Feedstocks and conversion technologies have, however, been the bottleneck to the realization of this concept. Herein, feedstocks and bioconversion technologies under biorefinery have been reviewed. Over the last decade, research has shown possibilities of generating tens of new products but only few industrial implementations. This is partly associated with low production yields and poor cost-competitiveness. This review addresses the technical barriers associated with the conversion of emerging feedstocks into chemicals and bioenergy platforms and summarizes the developed biotechnological approaches including advances in metabolic engineering. This summary further suggests possible future advances that would expand the portfolio of biorefinery and speed up the realization of biofuels and biochemicals.

  Lead, Jun. 2019, Biotechnology Journal, 14(6) (6), 1800494, English, International magazine

  [Refereed]

  Scientific journal


• 5-Hydroxymethylfurfural production from salt-induced photoautotrophically cultivated Chlorella sorokiniana

  Jerome Amoah, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  5-hydroxymethylfurfural (5-HMF), a key precursor to biochemicals has been produced from simple sugars. We report the production of 5-HMF directly from sustainable Chlorella sorokiniana cultivated in a saline medium supplied with CO2 as the sole carbon source. 43% carbohydrate and 16% lipid production in C. sorokiniana were achieved with an optimized CO2 concentration of 1%. 5-HMF yield of 52% was produced in a one-pot acid dehydration reactor at 150 °C in 2 h. 5-HMF yield from C. sorokiniana was almost a 40-fold higher than that from lignocellulose sugar cane bagasse – a highly abundant biomass. SEM images revealed that, the cells of C. sorokiniana could easily be broken in this one-pot reactor to liberate the built-up carbohydrate from the plastids of the cells for subsequent conversion to 5-HMF. The presence of lipids in C. sorokiniana was found to promote the conversion of starch to 5-HMF. Ultimately, the addition of LiCl was found to produce more than 2-fold 5-HMF from C. sorokiniana when compared to the absence of metal salts, with a 5-HMF selectivity of 98.7%. These findings suggest that, starch accumulating microalgae could be potential sustainable feedstock for 5-HMF production.

  Elsevier {BV}, Feb. 2019, Biochemical Engineering Journal, 142, 117 - 123, English

  Scientific journal


• GH-10 and GH-11 Endo-1,4-β-xylanase enzymes from Kitasatospora sp. produce xylose and xylooligosaccharides from sugarcane bagasse with no xylose inhibition.

  Rahmani N, Kahar P, Lisdiyanti P, Lee J, Yopi, Prasetya B, Ogino C, Kondo A

  A novel strategy for the low-cost, high-yield co-production of xylose and xylooligosaccharides together with no xylose inhibition was developed using a novel heterologous expression of XYN10Ks_480 endo-1,4-β-xylanase with a ricin-type β-trefoil type of domain and XYN11Ks_480 endo-1,4-β-xylanase with a CBM 2 superfamily from the Kitasatospora sp in an actinomycetes expression system. Xylose is the main building block for hemicellulose xylan. Our findings demonstrated high levels of expression and catalytic activity for XYN10Ks_480 during hydrolysis of the extracted xylan of bagasse, and three types of xylan-based substrates were used to produce xylose and xylooligosaccharides. However, hydrolysis by XYN11Ks_480 produced xylooligosaccharides without xylose formation. This study demonstrated how integrating sodium hypochlorite-extracted xylan and enzymatic hydrolysis could provide an alternative strategy for the generation of XOS from lignocellulosic material.

  Lead, Jan. 2019, Bioresource technology, 272, 315 - 325, English, International magazine

  [Refereed]

  Scientific journal


• Lipid production by Lipomyces starkeyi using sap squeezed from felled old oil palm trunks.

  Juanssilfero AB, Kahar P, Amza RL, Yopi, Sudesh K, Ogino C, Prasetya B, Kondo A

  The ability of oleaginous yeast Lipomyces starkeyi to efficiently produce lipids when cultivated on sap extracted from felled oil palm trunk (OPT) as a novel inexpensive renewable carbon source was evaluated. OPT sap was found to contain approximately 98 g/L glucose and 32 g/L fructose. Batch fermentations were performed using three different OPT sap medium conditions: regular sap, enriched sap, and enriched sap at pH 5.0. Under all sap medium conditions, the cell biomass and lipid production achieved were approximately 30 g/L and 60% (w/w), respectively. L. starkeyi tolerated acidified medium (initial pH ≈ 3) and produced considerable amounts of ethanol as well as xylitol as by-products. The fatty acid profile of L. starkeyi was remarkably similar to that of palm oil, one of the most common vegetable oil feedstock used in biodiesel production with oleic acid as the major fatty acid followed by palmitic, stearic and linoleic acids.

  Lead, Elsevier {BV}, Jan. 2019, Journal of bioscience and bioengineering, 127(6) (6), 726 - 731, English, Domestic magazine

  [Refereed]

  Scientific journal


• Development of a strictly regulated xylose-induced expression system in Streptomyces

  Yuji Noguchi, Norimasa Kashiwagi, Atsuko Uzura, Chiaki Ogino, Akihiko Kondo, Haruo Ikeda, Masahiro Sota

  Background: Genetic tools including constitutive and inducible promoters have been developed over the last few decades for strain engineering in Streptomyces. Inducible promoters are useful for controlling gene expression, however only a limited number are applicable to Streptomyces. The aim of this study is to develop a controllable protein expression system based on an inducible promoter using sugar inducer, which has not yet been widely applied in Streptomyces. Results: To determine a candidate promoter, inducible protein expression was first examined in Streptomyces avermitilis MA-4680 using various carbon sources. Xylose isomerase (xylA) promoter derived from xylose (xyl) operon was selected due to strong expression of xylose isomerase (XylA) in the presence of d-xylose. Next, a xylose-inducible protein expression system was constructed by investigating heterologous protein expression (chitobiase as a model protein) driven by the xylA promoter in Streptomyces lividans. Chitobiase activity was detected at high levels in S. lividans strain harboring an expression vector with xylA promoter (pXC), under both xylose-induced and non-induced conditions. Thus, S. avermitilis xylR gene, which encodes a putative repressor of xyl operon, was introduced into constructed vectors in order to control protein expression by d-xylose. Among strains constructed in the study, XCPR strain harboring pXCPR vector exhibited strict regulation of protein expression. Chitobiase activity in the XCPR strain was observed to be 24 times higher under xylose-induced conditions than that under non-induced conditions. Conclusion: In this study, a strictly regulated protein expression system was developed based on a xylose-induced system. As far as we could ascertain, this is the first report of engineered inducible protein expression in Streptomyces by means of a xylose-induced system. This system might be applicable for controllable expression of toxic products or in the field of synthetic biology using Streptomyces strains.

  Springer Nature, Dec. 2018, Microbial Cell Factories, 17(1) (1), English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Selection of oleaginous yeasts capable of high lipid accumulation during challenges from inhibitory chemical compounds

  Ario Betha Juanssilfero, Prihardi Kahar, Rezky Lastinov Amza, Nao Miyamoto, Hiromi Otsuka, Hana Matsumoto, Chie Kihira, Ahmad Thontowi, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  A two-stage selection process was applied to eight oleaginous yeast strains from the Lipomyces genera. In the primary selection stage, a nitrogen-limited mineral medium (-NMM) that contained a mixture of glucose and xylose as a carbon source was used to evaluate the lipid-accumulating abilities of the yeast strains. The strains L. doorenjongii, L. orientalis, and L. starkeyi were selected as the potential strains in the primary selection. These three strains exhibited a remarkable ability to simultaneously assimilate glucose and xylose and achieved a cell biomass of more than 30 g/L. The values for lipid content in the selected strains were 57.89 ± 1.92, 56.38 ± 1.93, and 77.14 ± 1.55% for L. doorenjongii, L. orientalis, and L. starkeyi, respectively. In the secondary selection, when the -NMM medium contained an inhibitory chemical compound (ICC), the selected strains showed a different tolerance level against each of the typical inhibitor compounds. However, L. starkeyi accumulated the highest lipid content and yield at 68.24 ± 2.48% and 0.19 ± 0.00 (w/w), respectively. L. starkeyi accumulated high levels of intracellular lipid and tolerated the ICC. The composition of fatty acid methyl esters (FAMEs) was unaltered by the presence of ICC and the major FAMEs consisted of oleic, palmitic, stearic, palmitoleic and linoleic acids.

  Lead, Elsevier B.V., Sep. 2018, Biochemical Engineering Journal, 137, 182 - 191, English

  [Refereed]


• Repeated ethanol fermentation from membrane-concentrated sweet sorghum juice using the flocculating yeast Saccharomyces cerevisiae F118 strain.

  Wijaya H, Sasaki K, Kahar P, Yopi, Kawaguchi H, Sazuka T, Ogino C, Prasetya B, Kondo A

  The aim of this study was to construct a cost-effective method for repeated bioethanol production using membrane (ultrafiltration permeation and nanofiltration concentration)-concentrated sweet sorghum juice by using flocculent Saccharomyces cerevisiae F118 strain. With low initial dry cell concentrations at around 0.28-0.35 g L-1, the S. cerevisiae F118 strain provided an ethanol titer of 86.19 ± 1.15 g L-1 (theoretical ethanol yield of 70.77%), which was higher than the non-flocculent S. cerevisiae BY4741 strain at 33.92 ± 0.99 g L-1 after 24 h fermentation. This result was correlated with higher gene expressions of the sucrose-hydrolysing enzyme invertase, sugar phosphorylation, and pyruvate-to-ethanol pathways in the F118 strain compared with the BY4741 strain. Sequential fed-batch fermentation was conducted, and the F118 strain was easily separated from the fermentation broth via the formation of flocs and sediment. After the 5th cycle of fermentation with the F118 strain, the ethanol concentration reached 100.37 g L-1.

  Lead, Jul. 2018, Bioresource technology, 265, 542 - 547, English, International magazine

  [Refereed]


• Efficient and supplementary enzyme cocktail from Actinobacteria and plant biomass induction.

  Takenaka M, Lee JM, Kahar P, Ogino C, Kondo A

  Actinobacteria plays a key role in the cycling of organic matter in soils. They secret biomass-degrading enzymes that allow it to produce the unique metabolites that originate in plant biomass. Although past studies have focused on these unique metabolites, a large-scale screening of Actinobacteria is yet to be reported to focus on their biomass-degrading ability. In the present study, a rapid and simple method is constructed for a large-scale screening, and the novel resources that form the plant biomass-degrading enzyme cocktail are identified from 850 isolates of Actinobacteria. As a result, Nonomuraea fastidiosa secretes a biomass degrading enzyme cocktail with the highest enzyme titer, although cellulase activities are lower than a commercially available enzyme. So the rich accessory enzymes are suggested to contribute to the high enzyme titer for a pretreated bagasse with a synergistic effect. Additionally, an optimized cultivation method of biomass induction caused to produce the improved enzyme cocktail indicated strong enzyme titers and a strong synergistic effect. Therefore, the novel enzyme cocktails are selected via the optimized method for large-scale screening, and then the enzyme cocktail can be improved via the optimized production with biomass-induction.

  Lead, Jul. 2018, Biotechnology journal, 14(3) (3), e1700744, English, International magazine

  [Refereed]

  Scientific journal


• Effect of inoculum size on single-cell oil production from glucose and xylose using oleaginous yeast Lipomyces starkeyi

  Ario B. Juanssilfero, Prihardi Kahar, Rezky L. Amza, Nao Miyamoto, Hiromi Otsuka, Hana Matsumoto, Chie Kihira, Ahmad Thontowi, Yopi, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo

  Oleaginous microbes can convert substrates such as carbon dioxide, sugars, and organic acids to single-cell oils (SCOs). Among the oleaginous microorganisms, Lipomyces starkeyi is a particularly well-suited host given its impressive native abilities, including the capability to utilize a wide variety of carbon sources. In this work, the potential of L. starkeyi NBRC10381 to produce SCOs in a synthetically nitrogen-limited mineral medium (-NMM) was investigated by differing the inoculum size using glucose and/or xylose as a carbon source. Fermentation using glucose and xylose as mixed carbon sources generated the highest production of biomass at 40.8 g/L, and achieved a lipid content of 84.9% (w/w). When either glucose or xylose was used separately, the totals for achieved lipid content were 79.6% (w/w) and 85.1% (w/w), respectively. However, biomass production was higher for glucose than for xylose (30.3 vs. 28.7 g/L, respectively). This study describes the first simultaneous achievement of higher levels of cell mass and lipid production using glucose and/or xylose as the carbon sources in different inoculum sizes.

  Lead, Elsevier B.V., Jun. 2018, Journal of Bioscience and Bioengineering, 125(6) (6), 695 - 702, English

  [Refereed]

  Scientific journal


• Xylanase and feruloyl esterase from actinomycetes cultures could enhance sugarcane bagasse hydrolysis in the production of fermentable sugars

  Nanik Rahmani, Prihardi Kahar, Puspita Lisdiyanti, Euis Hermiati, Jaemin Lee, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

  The addition of enzymes that are capable of degrading hemicellulose has a potential to reduce the need for commercial enzymes during biomass hydrolysis in the production of fermentable sugars. In this study, a high xylanase producing actinomycete strain (Kitasatospora sp. ID06-480) and the first ethyl ferulate producing actinomycete strain (Nonomuraea sp. ID06-094) were selected from 797 rare actinomycetes, respectively, which were isolated in Indonesia. The addition (30%, v/v) of a crude enzyme supernatant from the selected strains in sugarcane bagasse hydrolysis with low-level loading (1 FPU/g-biomass) of Cellic® CTec2 enhanced both the released amount of glucose and reducing sugars. When the reaction with Ctec2 was combined with crude enzymes containing either xylanase or feruloyl esterase, high conversion yield of glucose from cellulose at 60.5% could be achieved after 72 h-saccharification.

  Japan Society for Bioscience Biotechnology and Agrochemistry, 2018, Bioscience, Biotechnology and Biochemistry, 82(5) (5), 904 - 915, English

  [Refereed]

  Scientific journal


• Glutathione production from mannan-based bioresource by mannanase/mannosidase expressing Saccharomyces cerevisiae

  Alex Prima, Kiyotaka Y. Hara, Apridah Cameliawati Djohan, Norimasa Kashiwagi, Prihardi Kahar, Jun Ishii, Hideki Nakayama, Fumiyoshi Okazaki, Bambang Prasetya, Akihiko Kondo, Yopi, Chiaki Ogino

  This work aims to produce glutathione directly from mannan-based bioresources using engineered Saccharomyces cerevisiae. Mannan proved to be a valuable carbon source for glutathione production by this organism. Mannan-hydrolyzing S. cerevisiae was developed by heterologous expression of mannanase/ mannosidase on its cell surface. This strain efficiently produced glutathione from mannose polysaccharide, beta-1,4-mannan. Furthermore, it produced glutathione from locust bean gum (LBG), a highly dense and inexpensive mannan-based bioresource, as sole carbon source. Glutathione productivity from LBG was enhanced by engineering the glutathione metabolism of mannan-hydrolyzing S. cerevisiae. Expression of extracellular mannanase/mannosidase protein combined with intracellular metabolic engineering is potentially applicable to the efficient, environmentally friendly bioproduction of targeted products from mannan-based bioresources. (C) 2017 Elsevier Ltd. All rights reserved.

  ELSEVIER SCI LTD, Dec. 2017, BIORESOURCE TECHNOLOGY, 245(Pt B) (Pt B), 1400 - 1406, English

  [Refereed]


• Challenges of non-flocculating Saccharomyces cerevisiae haploid strain against inhibitory chemical complex for ethanol production

  Prihardi Kahar, Eny Ida Riyanti, Hiromi Otsuka, Hana Matsumoto, Chie Kihira, Chiaki Ogino, Akihiko Kondo

  This study provides insight observation based on the gene expression and the metabolomic analysis of the natural robust yeast Saccharomyces cerevisiae NBRC849 during the fermentation in the medium containing inhibitory chemical complexes (ICC) at different concentrations. The tolerance mechanisms involved in the strain might have existed through the upregulation of genes involved in NAD(H)/NADP (H) cofactors generations (ALD6, ZWF1, GND1), membrane robustness for efflux pump (YOR1, PDR5, TPO3) and cation/polyamine transport (TPO3). The alteration of metabolic flux to the shikimic pathway was also found in this strain, resulted in the enhanced formation of aromatic amino acid required for cell survival. Enhanced expression of these genes as well as the increase of metabolic flux to shikimic pathway were suggested to result in the robustness of non-flocculating S. cerevisiae haploid strain. (C) 2017 Elsevier Ltd. All rights reserved.

  Lead, ELSEVIER SCI LTD, Dec. 2017, BIORESOURCE TECHNOLOGY, 245(Pt B) (Pt B), 1436 - 1446, English

  [Refereed]

  Scientific journal


• Caffeic acid production by simultaneous saccharification and fermentation of kraft pulp using recombinant Escherichia coli

  Hideo Kawaguchi, Yohei Katsuyama, Du Danyao, Prihardi Kahar, Sachiko Nakamura-Tsuruta, Hiroshi Teramura, Keiko Wakai, Kumiko Yoshihara, Hiromichi Minami, Chiaki Ogino, Yasuo Ohnishi, Ahikiko Kondo

  Caffeic acid (3,4-dihydroxycinnamic acid) serves as a building block for thermoplastics and a precursor for biologically active compounds and was recently produced from glucose by microbial fermentation. To produce caffeic acid from inedible cellulose, separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) reactions were compared using kraft pulp as lignocellulosic feedstock. Here, a tyrosine-overproducing Escherichia coli strain was metabolically engineered to produce caffeic acid from glucose by introducing the genes encoding a 4-hydroxyphenyllactate 3-hydroxylase (hpaBC) from Pseudomonas aeruginosa and tyrosine ammonia lyase (fevV) from Streptomyces sp. WK-5344. Using the resulting recombinant strain, the maximum yield of caffeic acid in SSF (233 mg/L) far exceeded that by SHF (37.9 mg/L). In the SSF with low cellulase loads (ae<currency>2.5 filter paper unit/g glucan), caffeic acid production was markedly increased, while almost no glucose accumulation was detected, indicating that the E. coli cells experienced glucose limitation in this culture condition. Caffeic acid yield was also negatively correlated with the glucose concentration in the fermentation medium. In SHF, the formation of by-product acetate and the accumulation of potential fermentation inhibitors increased significantly with kraft pulp hydrolysate than filter paper hydrolysate. The combination of these inhibitors had synergistic effects on caffeic acid fermentation at low concentrations. With lower loads of cellulase in SSF, less potential fermentation inhibitors (furfural, 5-hydroxymethyfurfural, and 4-hydroxylbenzoic acid) accumulated in the medium. These observations suggest that glucose limitation in SSF is crucial for improving caffeic acid yield, owing to reduced by-product formation and fermentation inhibitor accumulation.

  SPRINGER, Jul. 2017, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 101(13) (13), 5279 - 5290, English

  [Refereed]

  Scientific journal


• Mannan endo-1,4-beta-mannosidase from Kitasatospora sp isolated in Indonesia and its potential for production of mannooligosaccharides from mannan polymers

  Nanik Rahmani, Norimasa Kashiwagi, JaeMin Lee, Satoko Niimi-Nakamura, Hana Matsumoto, Prihardi Kahar, Puspita Lisdiyanti, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

  Mannan endo-1,4-beta-mannosidase(commonly known as beta-mannanase) catalyzes a random cleavage of the beta-D-1,4mannopyranosyl linkage in mannan polymers. The enzyme has been utilized in biofuel production from lignocellulose biomass, as well as in production of mannooligosaccharides (MOS) for applications in feed and food industries. We aimed to obtain a beta-mannanase, for such mannan polymer utilization, from actinomycetes strains isolated in Indonesia. Strains exhibiting high mannanase activity were screened, and one strain belonging to the genus Kitasatospora was selected. We obtained a beta-mannanase from this strain, and an amino acid sequence of this Kitasatospora beta-mannanase showed a 58-71% similarity with the amino acid sequences of Streptomyces beta-mannanases. The Kitasatospora beta-mannanase showed a significant level of activity (944 U/mg) against locust bean gum (0.5% w/v) and a potential for oligosaccharide production from various mannan polymers. The beta-mannanase might be beneficial particularly in the enzymatic production of MOS for applications of mannan utilization.

  BIOMED CENTRAL LTD, May 2017, AMB EXPRESS, 7(1) (1), 100, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A Systematic Approach to Timeseries Metabolite Profiling and RNA-seq Analysis of Chinese Hamster Ovary Cell Culture

  Han-Hsiu Hsu, Michihiro Araki, Masao Mochizuki, Yoshimi Hori, Masahiro Murata, Prihardi Kahar, Takanobu Yoshida, Tomohisa Hasunuma, Akihiko Kondo

  Chinese hamster ovary (CHO) cells are the primary host used for biopharmaceutical protein production. The engineering of CHO cells to produce higher amounts of biopharmaceuticals has been highly dependent on empirical approaches, but recent high-throughput "omics" methods are changing the situation in a rational manner. Omics data analyses using gene expression or metabolite profiling make it possible to identify key genes and metabolites in antibody production. Systematic omics approaches using different types of time-series data are expected to further enhance understanding of cellular behaviours and molecular networks for rational design of CHO cells. This study developed a systematic method for obtaining and analysing time-dependent intracellular and extracellular metabolite profiles, RNA-seq data (enzymatic mRNA levels) and cell counts from CHO cell cultures to capture an overall view of the CHO central metabolic pathway (CMP). We then calculated correlation coefficients among all the profiles and visualised the whole CMP by heatmap analysis and metabolic pathway mapping, to classify genes and metabolites together. This approach provides an efficient platform to identify key genes and metabolites in CHO cell culture.

  NATURE PUBLISHING GROUP, Mar. 2017, SCIENTIFIC REPORTS, 7, 43518, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• From mannan to bioethanol: cell surface co-display of beta-mannanase and beta-mannosidase on yeast Saccharomyces cerevisiae

  Jun Ishii, Fumiyoshi Okazaki, Apridah Cameliawati Djohan, Kiyotaka Y. Hara, Nanami Asai-Nakashima, Hiroshi Teramura, Ade Andriani, Masahiro Tominaga, Satoshi Wakai, Prihardi Kahar, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

  Background: Mannans represent the largest hemicellulosic fraction in softwoods and also serve as carbohydrate stores in various plants. However, the utilization of mannans as sustainable resources has been less advanced in sustainable biofuel development. Based on a yeast cell surface-display technology that enables the immobilization of multiple enzymes on the yeast cell walls, we constructed a recombinant Saccharomyces cerevisiae strain that co-displays beta-mannanase and beta-mannosidase; this strain is expected to facilitate ethanol fermentation using mannan as a biomass source. Results: Parental yeast S. cerevisiae assimilated mannose and glucose as monomeric sugars, producing ethanol from mannose. We constructed yeast strains that express tethered beta-mannanase and beta-mannosidase; co-display of the two enzymes on the cell surface was confirmed by immunofluorescence staining and enzyme activity assays. The constructed yeast cells successfully hydrolyzed 1,4-beta-D-mannan and produced ethanol by assimilating the resulting mannose without external addition of enzymes. Furthermore, the constructed strain produced ethanol from 1,4-beta-D-mannan continually during the third batch of repeated fermentation. Additionally, the constructed strain produced ethanol from ivory nut mannan; ethanol yield was improved by NaOH pretreatment of the substrate. Conclusions: We successfully displayed beta-mannanase and beta-mannosidase on the yeast cell surface. Our results clearly demonstrate the utility of the strain co-displaying beta-mannanase and beta-mannosidase for ethanol fermentation from mannan biomass. Thus, co-tethering beta-mannanase and beta-mannosidase on the yeast cell surface provides a powerful platform technology for yeast fermentation toward the production of bioethanol and other biochemicals from lignocellulosic materials containing mannan components.

  BIOMED CENTRAL LTD, Sep. 2016, BIOTECHNOLOGY FOR BIOFUELS, 9(1) (1), 188, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse

  Hideo Kawaguchi, Hiroshi Teramura, Kouji Uematsu, Kiyotaka Y. Hara, Tomohisa Hasunuma, Ko Hirano, Takashi Sazuka, Hidemi Kitano, Yota Tsuge, Prihardi Kahar, Satoko Niimi-Nakamura, Ken-Ichi Oinuma, Naoki Takaya, Shigemitsu Kasuga, Chiaki Ogino, Akihiko Kondo

  Dilute acid-pretreated sorghum bagasse, which was predominantly composed of glucan (59%) and xylose (7.2%), was used as a lignocellulosic feedstock for D-phenyllactic acid (PhLA) production by a recombinant Escherichia coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens. During fermentation with enzymatic hydrolysate of sorghum bagasse as a carbon source, the PhLA yield was reduced by 35% compared to filter paper hydrolysate, and metabolomics analysis revealed that NAD(P)H regeneration and intracellular levels of erythrose-4-phosphate and phosphoenolpyruvate for PhLA biosynthesis markedly reduced. Compared to separate hydrolysis and fermentation (SHF) with sorghum bagasse hydrolysate, simultaneous saccharification and fermentation (SSF) of sorghum bagasse under glucose limitation conditions yielded 4.8-fold more PhLA with less accumulation of eluted components, including p-coumaric acid and aldehydes, which inhibited PhLA fermentation. These results suggest that gradual enzymatic hydrolysis during SSF enhances PhLA production under glucose limitation and reduces the accumulation of fermentation inhibitors, collectively leading to increased PhLA yield. (c) 2015 Elsevier Ltd. All rights reserved.

  ELSEVIER SCI LTD, Apr. 2015, BIORESOURCE TECHNOLOGY, 182, 169 - 178, English

  [Refereed]

  Scientific journal


• A xylose-fermenting yeast hybridized by intergeneric fusion between Saccharomyces cerevisiae and Candida intermedia mutants for ethanol production.

  PRIHARDI KAHAR, SHUZO TANAKA

  Bioethanol production from lignocellulosic biomass, in particular xylose, is currently of great concern, given the abundance of this sugar in the world, because Saccharomyces cerevisiae, which is widely used for bioethanol production, is unable to naturally ferment xylose. The aim of this study was to obtain a novel yeast capable of stably producing ethanol from biomass contain

  Lead, Springer, 2014, Sustainable Chemical Processes, 2(17) (17), 1 - 12, English

  [Refereed]

  Scientific journal


• Multiple effects of swelling by sodium bicarbonate after delignification on enzymatic saccharification of rice straw

  Prihardi Kahar, Kazuo Taku, Shuzo Tanaka

  The multiple effects of pretreatments by chemical delignification using acidified sodium chlorite (ASC) and swelling using sodium bicarbonate (SB) for enzymatic saccharification of rice straw in bioethanol production have been investigated in this study. The treatment with the combination of ASC three times (3 x ASC) first and SB later resulted in the significant reduction in Mason lignin content up to 90% (wt./wt.). By the saccharification of the pretreated rice straw with cellulase enzymes, it was confirmed that SB treatment was an important step in the pretreatment process not only to disintegrate the cellulose structure but also to facilitate the amorphization of the crystalline cellulose as well as the extended removal of integrated lignin. Furthermore, FTIR analyses revealed that the crystal type of cellulose appeared to be changed from type I to type II by SB treatment, thereby increasing the cellulose surface area and making it more accessible to the cellulase enzyme. Conversion rate to sugar was remarkably increased when 3x ASC + SB treatments were applied to untreated rice straw, even though the saccharification of the treated rice straw was performed at a low enzyme loading (1/100, wt.-enzymes/wt.-substrate). Conclusively, rice straw could be saccharified at high yield in short time at low cellulase loading, enables the enzymatic saccharification to be more feasible for practical bioethanol production using rice straw as a substrate. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.

  SOC BIOSCIENCE BIOENGINEERING JAPAN, Dec. 2013, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 116(6) (6), 725 - 733, English

  [Refereed]

  Scientific journal


• Enhancement of xylose uptake in 2-deoxyglucose tolerant mutant of Saccharomyces cerevisiae

  Prihardi Kahar, Kazuo Taku, Shuzo Tanaka

  Chemical mutation of Saccharomyces cerevisiae using ethyl methane sulfonate was performed to enhance its ability of xylose uptake for ethanol production from lignocellulose under microaerobic condition. Among the appeared mutants, the mutant no. 2 (M2) strain screened using inhibitory effects of 2-deoxyglucose (DOG) showed more than 4-fold high ability in xylose uptake compared with the wild type strain, under the presence of glucose. The catabolite repression by glucose was sufficiently reduced in M2 strain due to its tolerance to the high concentration of DOG (0.5%, wt./vol.). Metabolomic analyses of various sugars in the cell revealed that some of xylose was reduced to xylitol in M2 cell, providing the concentration gradient of xylose and more uptake of xylose. Xylulose-5-phosphate was significantly detected in the crude cell extract from M2 strain, indicating higher metabolic activity in pentose phosphate pathway. This was also confirmed by in vitro analyses of key enzymes involved in glucose and xylose metabolism, such as hexokinase, glucose-6-phosphate dehydrogenase and xylose reductase. Glucose uptake was moderately suppressed in the presence of trehalose-6-phosphate inhibiting the activation of hexokinase, resulting in more uptake of xylose through hexose transport system. To our knowledge, this study is the first report verifying that the mutation technique successfully enhances the xylose uptake by S. cerevisiae, particularly under the presence of glucose. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.

  SOC BIOSCIENCE BIOENGINEERING JAPAN, May 2011, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 111(5) (5), 557 - 563, English

  [Refereed]

  Scientific journal


• Unusual change in molecular weight of polyhydroxyalkanoate (PHA) during cultivation of PHA-accumulating Escherichia coli

  Jumiarti Agus, Prihardi Kahar, Manami Hyakutake, Satoshi Tomizawa, Hideki Abe, Takeharu Tsuge, Yasuharu Satoh, Kenji Tajima

  This study aimed to investigate the factors affecting molecular weight of poly when polyhydroxyalkanoate (PHA) synthase (PhaRC(Bsp)) from Bacillus sp. INT005 was used for P(3HB) synthesis in Escherichia coli JM109. It was found that the molecular weight of P(3HB) decreased with time in mid- and late-phase of culture and was strongly affected by culture temperature. At 37 degrees C culture temperature, the molecular weight of P(3HB) rapidly decreased from 4.4 x 10(5) to 4.8 x 10(4) with culture time, whereas it was almost unchanged at 25 degrees C. Kinetic analysis suggested that the decrease in molecular weight of P(3HB) was due to random scission of the polymer chain. The decrease in molecular weight of P(3HB) was not observed when PHA synthases other than PhaRC(Bsp) were expressed. This study sheds light on the unique behaviour in molecular weight change of P(3HB) that is synthesized by E. coli expressing PhaRCB(sp). (C) 2010 Elsevier Ltd. All rights reserved.

  ELSEVIER SCI LTD, Dec. 2010, POLYMER DEGRADATION AND STABILITY, 95(12) (12), 2250 - 2254, English

  Scientific journal


• Isolation and purification of novel thermostable alkaline lipase from local thermophilic microorganism

  Febriani, Hertadi, R., Kahar, P., Akhmaloka, Madayanti, F.

  A local thermophilic microorganism namely DMS-3 has been identified as thermostable alkaline lipase producing isolate. The bacterium was isolated from Domas hot spring, Tangkuban Perahu Mount, West Java. Based on gram staining test and Scanning Electron Microscopy, the microorganism showed a rod shape and a gram positive bacterium. The isolated showed maximum expression of lipase at pH 9 and 70°C. The lipase produced by DMS-3 isolate was purified using column chromatography of DEAE sepharose fast flow and Sephacryl S-200. Following Sephacryl separation the enzyme still showed specific activity at 54 times higher compared to that the wild type, with yield was about 4.89%. Polyacryamide gel electrophoresis combining with zymogram analysis showed that there were 4 bands of protein exhibiting lipase activity. Further analysis by eluting of each band showed that the first, second, and third band revealed few bands similar of each other on denatured SDS-PAGE. Meanwhile the fourth band only showed single band. The data suggested that DMS-3 isolated expressed more than one type of lipases.

  Dec. 2010, Biosciences Biotechnology Research Asia, 7(2) (2), 617 - 622

  Scientific journal


• Improved utilization of xylose by mutated-fused yeast for bioethanol production

  P. Kahar, S. Tanaka

  Lead, ELSEVIER SCIENCE BV, Nov. 2010, JOURNAL OF BIOTECHNOLOGY, 150, S136 - S137, English


• Enzymatic digestion of corncobs pretreated with low strength of sulfuric acid for bioethanol production

  Prihardi Kahar, Kazuo Taku, Shuzo Tanaka

  In this study, the effect and the optimum pretreatment condition of corncobs using low strength of H(2)SO(4) were investigated, in which H(2)SO(4) was used to improve the enzymatic digestibility of corncobs for saccharification without degradation of sugars released. The optimum pretreatment condition was found to be the addition of 0.5% (vol./vol.) H(2)SO(4) and autoclaving at 122 degrees C for 20 min. Under this condition, the structural integrity of corncob was altered to make cellulose microfibrils more accessible for cellulase enzymes, and the enzymatic digestion of corncobs could be significantly enhanced. A high yield of sugar, 80% (wt./wt.), could be obtained at a low enzyme dosage of 0.024 g enzymes/g cobs, when pretreated. As a result, the ethanol production was obviously improved by the pretreatment, i.e., the ethanol yield of 77% (wt./wt.) was obtained within 36 h in the SSF fermentation using Saccharomyces cerevisiae NBRC2114. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.

  SOC BIOSCIENCE BIOENGINEERING JAPAN, Oct. 2010, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 110(4) (4), 453 - 458, English

  [Refereed]

  Scientific journal


• On the swiveling motion of helices of c-subunit in rotation of ATP synthase

  Prihardi Kahar, Toshiharu Suzuki, Masasuke Yoshida

  Lead, BIOPHYSICAL SOCIETY, Jan. 2007, BIOPHYSICAL JOURNAL, -, 146A - 146A, English


• Molecular weight study of poly[(r)-3-hydroxybutyrate] synthesized by recombinant Escherichia coli

  Takeharu Tsuge, Jumiarti Agus, Prihardi Kahar, Hideki Abe, Hideki Abe

  Polyhydroxyalkanoate (PHA) synthase (PhaC) from Wautersia eutropha was expressed in a wide range of production level in Escherichia coli XL1-Blue cells and its effects on PhaC activity, poly production and its molecular weights were investigated. The production level of PhaC was controlled by both amount of chemical inducer (IPTG) added into the medium and use of different copy number of plasmids. In a flask experiment, as increasing PhaC production level in the cells, the PhaC activity also increased in the range of low PhaC concentration. However, PhaC activity did not further increase in the range of high PhaC concentration, probably due to formation of inclusion body in the cells. The molecular weight of P(3HB) was found to decrease with increasing PhaC activity. This trend was also verified in high cell density cultivation using 10-l jar fermentor. Furthermore, we demonstrated that use of low copy number plasmid and appropriate induction of PhaC expression were effective in achieving both high productivity and high molecular weight of P(3HB).

  Oct. 2006, Polymer Preprints, Japan, 55, 2204


• Altered expression of polyhydroxyalkanoate synthase gene and its effect on poly[(R)-3-hydroxybutyrate] synthesis in recombinant Escherichia coli

  J Agus, P Kahar, H Abe, Y Doi, T Tsuge

  Polyhydroxyalkanoate (PHA) synthase (PhaC) from Wautersia eutropha was expressed in a wide range of production level in Escherichia coli XL1-Blue cells and its effects on PhaC activity, poly production and its molecular weights were investigated. The production level of PhaC was controlled both by the amount of chemical inducer (isopropyl-beta-D-thiogalactopyranoside, IPTG) added into the medium and the use of different copy number of plasmids. In a flask experiment, as PhaC production level in the cells increased, the PhaC activity also increased in the range of low PhaC concentration. However, PhaC activity did not further increase in the range of high PhaC concentration, probably due to the formation of inclusion body in the cells. The molecular weight of P(3HB) was found to decrease with increasing PhaC activity. This trend was also verified in high cell density cultivation using 10-1 jar fermentor. Furthermore, we demonstrated that the use of low copy number plasmid and appropriate induction of PhaC expression were effective in achieving both high productivity and high molecular weight of P(3HB). (c) 2006 Elsevier Ltd. All rights reserved.

  ELSEVIER SCI LTD, Aug. 2006, POLYMER DEGRADATION AND STABILITY, 91(8) (8), 1645 - 1650, English

  Scientific journal


• Molecular weight characterization of poly[(R)-3-hydroxybutyrate] synthesized by genetically engineered strains of Escherichia coli

  J Agus, P Kahar, H Abe, Y Doi, T Tsuge

  This study investigated the relationship of growth conditions, host strains and molecular weights of poly synthesized by genetically engineered Escherichia coli. Various PHA synthases belonging to types I-IV enzymes were expressed in E. coli JM109 under the same experimental conditions, and the molecular weights of the polymers were characterized by gel permeation chromatography. The results demonstrate that P(3HB) polymers have varied molecular weights and polydispersities dependent on the characteristics of the individual PHA synthase employed. P(3HB) with high number-average molecular weights (Mn) [(1.5-4.0) X 10(6)] and narrow polydispersities (1.6-1.8) were synthesized by PHA synthases from Ralstonia eutropha (type 1), Delftia acidovorans (type 1) and Allochromatium vinosum (type 111). Contrary to these, P(3HB) with relatively low M-n [(0.17-0.79) X 10(6)] and broad polydispersities (2.2-9.0) were synthesized by PHA synthases from Aeromonas caviae (type I), Pseudomonas sp. 61-3 (type II) and Bacillus sp. INT005 (type IV). Furthermore, the molecular weights of P(3HB) synthesized under various culture conditions, in various hosts of E. coli and by mutants of PHA synthase were characterized. It was found that, in addition to culture pH [Kusaka et al. Appl Microbiol Biotechnol 1997;47:140], other variances such as culture temperature, host strain and use of mutants are effective in changing polymer molecular weight. (c) 2005 Elsevier Ltd. All rights reserved.

  ELSEVIER SCI LTD, May 2006, POLYMER DEGRADATION AND STABILITY, 91(5) (5), 1138 - 1146, English

  Scientific journal


• A crucial clue to understand the molecular rotation of c-subunit ring coupled with proton translocation through F(0) of thermophilic Bacillus PS3 ATP synthase

  P. Kahar, T. Suzuki, M. Yoshida

  Lead, ELSEVIER SCIENCE BV, 2006, BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 309 - 310, English


• Effect of PHA synthase activity and its concentration on the molecular weight of poly[(R)-3-hydroxybutyrate] synthesized in recombinant Escherichia coli

  Prihardi Kahar, Jumiarti Agus, Takeharu Tsuge, Yoshiharu Doi

  In this study, we have investigated the modulation effect of PHA synthase concentration level and its enzyme activity on the molecular weight of P(3HB) homopolymer synthesized in recombinant Escherichia coli. We found that P(3HB) with very high molecular weight could be synthesized whenever the concentration of PHA synthase within the cells was low. The molecular weights of P(3HB) showed a trend to become low whenever the concentration of PHA synthase was high, although there were remarkable increases in polymer content. Furthermore, the enzyme activity of PHA synthase was also determined and compared with flask and jar-scale experiments.

  2005, Polymer Preprints, Japan, 54(1) (1), 2012, Japanese

  International conference proceedings


• Molecular weight of poly[(R)-3-hydroxybutyrate] synthesized by type IV PHA synthase in Escherichia coli

  Jumiarti Agus, Prihardi Kahar, Takeharu Tsuge, Yoshiharu Doi

  In this study, we investigated the molecular weight of P(3HB) synthesized by the type IV PHA synthase from Bacillus sp. INT005 (PhaRC Bs). The PhaRC Bs preferred to synthesize low molecular weight of P(3HB) in Escherichia coli. The curve of molecular weight distribution was found to depend on cultivation time. Host strain of E. coli also affected the molecular weight of polymer, but culture temperature did not.

  2005, Polymer Preprints, Japan, 54(1) (1), 2014, Japanese


• Effective production and kinetic characterization of ultra-high-molecular-weight poly[(R)-3-hydroxybutyrate] in recombinant Escherichia coli

  P Kahar, J Agus, Y Kikkawa, K Taguchi, Y Doi, T Tsuge

  An inducible and highly effective production of ultra-high-molecular-weight poly[(R)-3-hydroxybutyrate] [UHMW-P(3HB)] in recombinant Escherichia coli XL1-Blue was investigated. Two expression plasmids harbouring Ralstonia eutropha P(3HB) biosynthesis genes (phaCAB(Re)) downstream of an inducible trc promoter, pTrcphaCAB(Re) and pJRDTrcphaCAB(Re) were constructed using the high copy number plasmid pTrc99a and the low copy number plasmid pJRDTrc1, respectively. These plasmids and the constitutive expression plasmid harbouring phaCAB(Re) genes (pSYL105) were individually introduced into E. coli XL1-Blue, and a comparative study for P(3HB) production from glucose using a 10-1 fermentor was carried out. The highest P(3HB) productivity (2.8 g/l h) was obtained when the recombinant harbouring pJRDTrcphaCAB(Re) was cultured with the induction of gene expression. The produced P(3HB) had extremely high weight-average molecular weights ranging from 3.5 x 10 to 5.0 x 10(6). with relatively low polydispersities of around 1.5. The molecular weights remained nearly unchanged during the course of cultivation from 12 to 54 h, suggesting that a chain-transfer reaction took place to regulate the length of polymer chains within the E. coli cells. (C) 2004 Elsevier Ltd. All rights reserved.

  Lead, ELSEVIER SCI LTD, Jan. 2005, POLYMER DEGRADATION AND STABILITY, 87(1) (1), 161 - 169, English

  Scientific journal


• High yield production of polyhydroxyalkanoates from soybean oil by Ralstonia eutropha and its recombinant strain

  P Kahar, T Tsuge, K Taguchi, Y Doi

  High yield production of polyhydroxyalkanoates (PHAs) by Ralstonia eutropha H16 and its recombinant strain PHB(-)4/pJRDEE32d13 (a PHA-negative mutant harboring Aeromonas caviae PHA synthase gene, phaC(Ac)) from renewable inexpensive soybean oil was investigated. The PHA production by the wild-type strain H16 was achieved with a high dry cells weight (118-126 g/l) and a high poly content per dry cells of 72-76% (w/w). A copolymer of 3HB with 5 mol% (R)-3-hydroxyhexanoate, P(3HB-co-5 mol% 3HHx), could be produced from soybean oil as a sole carbon source by the recombinant strain PHB(-)4/pJRDEE32d13 with a high dry cells weight (128-138 g/l) and a high PHA content of 71-74% (w/w). The reproducible results of PHA production in the presence of soybean oil as a sole carbon source was obtained with a high yield at a range of 0.72 to 0.76 g-PHA per g-soybean oil used. (C) 2003 Elsevier Ltd. All rights reserved.

  Lead, ELSEVIER SCI LTD, Jan. 2004, POLYMER DEGRADATION AND STABILITY, 83(1) (1), 79 - 86, English

  Scientific journal


• Purification and characterization of cis-aconitic acid decarboxylase from Aspergillus terreus TN484-M1

  L Dwiarti, K Yamane, H Yamatani, P Kahar, M Okabe

  cis-Aconitic acid decarboxylase (CAD) was assumed to be a key enzyme in the production of itaconic acid by comparing the activity of CAD from Aspergillus terreus TN484-M1 with that of CAD from the low-itaconate yielding strain Aspergillus terreus CM85J. The constitutive CAD was purified to homogeneity from A. terreus TN484-M1 by ammonium sulfate fractionation, and column chromatography on DEAE-toyopearl, Butyl-toyopearl, and Sephacryl S200HR, and then characterized. A molecular mass of 55 kDa for the native enzyme was determined by SDS-PAGE. The enzymic activity was optimal at a pH of 6.2 and temperature of 45degreesC. The K-m value for cis-aconitic acid was determined as 2.45 mM (pH 6.2, 37degreesC). The enzyme was completely inactivated by Hg+, Cu2+, Zu(2+),p-chloromercuribenzoate, and 5,5'-dithio-bis(2-nitrobenzoate).

  SOC BIOSCIENCE BIOENGINEERING JAPAN, Jul. 2002, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 94(1) (1), 29 - 33, English

  [Refereed]

  Scientific journal


• Purification and characterization of cis-aconitic acid decarboxylase from Aspergillus terreus TN484-M1

  L Dwiarti, K Yamane, H Yamatani, P Kahar, M Okabe

  cis-Aconitic acid decarboxylase (CAD) was assumed to be a key enzyme in the production of itaconic acid by comparing the activity of CAD from Aspergillus terreus TN484-M1 with that of CAD from the low-itaconate yielding strain Aspergillus terreus CM85J. The constitutive CAD was purified to homogeneity from A. terreus TN484-M1 by ammonium sulfate fractionation, and column chromatography on DEAE-toyopearl, Butyl-toyopearl, and Sephacryl S200HR, and then characterized. A molecular mass of 55 kDa for the native enzyme was determined by SDS-PAGE. The enzymic activity was optimal at a pH of 6.2 and temperature of 45degreesC. The K-m value for cis-aconitic acid was determined as 2.45 mM (pH 6.2, 37degreesC). The enzyme was completely inactivated by Hg+, Cu2+, Zu(2+),p-chloromercuribenzoate, and 5,5'-dithio-bis(2-nitrobenzoate).

  SOC BIOSCIENCE BIOENGINEERING JAPAN, Jul. 2002, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 94(1) (1), 29 - 33, English

  [Refereed]

  Scientific journal


• Production of epsilon-polylysine in an airlift bioreactor (ABR)

  P Kahar, K Kobayashi, T Iwata, J Hiraki, M Kojima, M Okabe

  This paper deals with studies on epsilon-poly-L-lysine (epsilon-PL) production in an airlift bioreactor (ABR) using Streptomyces albulus S410 (S410) to minimize the production cost including the downstream processing of epsilon-PL. In a 5-l ABR, 30 g/l of epsilon-PL was produced with a power consumption of 0.3 kW/m(3), the production level being similar to that in a 5-l jar fermentor with a power consumption of 8.0 kW/m(3). Furthermore, the leakage of intracellular nucleic acid (INA)-related substances into the culture broth in the ABR was less than that in the jar fermentor. Due to the high-level power consumption (8.0 kW/m(3)) in the jar fermentor, the morphology of the cells changed from the pellet to filament form due to the extensive shear stress arising from continuous agitation, thereby increasing the leakage of the INA-related substances into the culture broth. This suggested that ABR would have an advantage in the low-cost production of epsilon-PL over stirred tank type reactors (STR).

  Lead, SOC BIOSCIENCE BIOENGINEERING JAPAN, Mar. 2002, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 93(3) (3), 274 - 280, English

  [Refereed]

  Scientific journal


• An Optimization of epsilon-Polylysine Production by Streptomyces albulus Strain.

  PRIHARDI KAHAR

  Epsilon-poly-L-lysine(以下ePL)は､アルカロイド生産菌の検索の過程で､Streptomyces albulusの生産するL-lysineのホモポリマー(n=25-30)として酒井らにより初めて見出されたePLは必須アミノ酸であるリジンのホモポリマーであるので安全性が高くかつカチオン含量が高いので､さらにHレタリー用品､食品添加物､化粧品､医寮晶､農薬晶､電子材料などの広範な用途が期待され､ePLの工業生産プロセスが重要になってくる｡我々は､培養中のpHやグルコースの残存濃度などの培養条件を検討し､ePLの高収率生産プロセスを構築することを試みた｡さらに､低コスト化に向けた高純度など一PL生産システムの構築を実現するためにエアリフト型バイオリアククーでの発酵生産を検討した｡

  岐阜大学機関リポジトリ, 2002, 農学 甲第275号, English

  [Refereed]

  Doctoral thesis


• Optimization of tylosin feeding rate profile in production of acetyl-isovaleryl tylosin (AIV) from tylosin by Streptomyces thermotolerans YN554

  GW Huang, M Okabe, P Kahar, H Tsunekawa, Y Park

  An optimal feed rate profile of a substrate (tylosin) for a novel antibiotic, acetyl-isovaleryl tylosin (AIV) production process was investigated. In the first step of optimization, a kinetic model for production of AIV from tylosin by Streptomyces thermotolerans was established properly using the least square method, followed by the confirmation that the proposed model could be used to predict the production process of AIV from tylosin. An objective function, state equations and an inequality constraint with respect to the tylosin feeding rate profile were applied to maximize the amount of AIV produced from tylosin in a fed-batch culture. The optimized tylosin feeding rate profile was determined using a direct iterative search algorithm based on the modified complex method. The simulation of AIV production at the optimal tylosin feeding profile indicates that the final amount of AIV is expected to be about 30% higher than that at the conventional constant tylosin feeding rate, which was also confirmed experimentally using a 30-l jar fermentor.

  SOC BIOSCIENCE BIOENGINEERING JAPAN, May 2001, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 91(5) (5), 504 - 508, English

  Scientific journal


• Enhancement of epsilon-polylysine production by Streptomyces albulus strain 410 using pH control

  P Kahar, T Iwata, J Hiraki, EY Park, M Okabe

  The enhancement of epsilon -poly-L-lysine (epsilon -PL) production by Streptomyces albulus strain no. 410 (S410) by means of a pH control strategy was investigated. S140 cells produce epsilon -PL at a high concentration if the culture pH remains at about 4.0; however, if it shifts to higher than 4.0, the accumulated epsilon -PL is depolymerized. We therefore suggest a pH control strategy for cell growth and epsilon -PL production aimed at increasing the amount of epsilon -PL produced. The cultivation was divided into two control phases. In phase I, cell growth was accelerated by maintaining the pH at higher than 5.0; in phase II, epsilon -PL production was increased by maintaining the pH at about 4.0. To avoid an increase in the pH during phase II as a result of glucose depletion, the glucose concentration was kept at around 10 g/l by glucose feeding. This control strategy enhanced the production of h-PL to 48.3 g/l from 5.7 g/l in the case of batch culture.

  SOC BIOSCIENCE BIOENGINEERING JAPAN, Feb. 2001, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 91(2) (2), 190 - 194, English

  [Refereed]

  Scientific journal


• Enhancement of epsilon-polylysine production by Streptomyces albulus strain 410 using pH control

  P Kahar, T Iwata, J Hiraki, EY Park, M Okabe

  The enhancement of epsilon -poly-L-lysine (epsilon -PL) production by Streptomyces albulus strain no. 410 (S410) by means of a pH control strategy was investigated. S140 cells produce epsilon -PL at a high concentration if the culture pH remains at about 4.0; however, if it shifts to higher than 4.0, the accumulated epsilon -PL is depolymerized. We therefore suggest a pH control strategy for cell growth and epsilon -PL production aimed at increasing the amount of epsilon -PL produced. The cultivation was divided into two control phases. In phase I, cell growth was accelerated by maintaining the pH at higher than 5.0; in phase II, epsilon -PL production was increased by maintaining the pH at about 4.0. To avoid an increase in the pH during phase II as a result of glucose depletion, the glucose concentration was kept at around 10 g/l by glucose feeding. This control strategy enhanced the production of h-PL to 48.3 g/l from 5.7 g/l in the case of batch culture.

  SOC BIOSCIENCE BIOENGINEERING JAPAN, Feb. 2001, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 91(2) (2), 190 - 194, English

  Scientific journal


• Effect of soybean oil on oxygen transfer in the production of tetracycline with an airlift bioreactor

  S Jia, GB Chen, P Kahar, DB Choi, M Okabe

  Corn starch and soybean oil are suitable carbon sources for the production of tetracycline by Streptomyces aureofacience CG-1. However, it could not produce more than 6 g/l of tetracycline even if initial corn starch concentration was increased to more than 100 g/l. It was confirmed by shaking flask experiments that the k(L)a in a mixture of 2% soybean oh in water was four folds compared with that without soybean oil. With the addition of soybean oil to the starch medium in a shaking flask, tetracycline production was significantly improved. By scaling-up to a 5.5-l airlift bioreactor from 500-ml Erlenmeyer flask, more than 10 g/l of tetracycline was produced with the addition of 60 g/l of soybean oil to the medium containing 100 g/l of corn starch. The dissolved oxygen level in the airlift bioreactor containing soybean oil was higher than that without soybean oil. This suggests that soybean oil is not only a suitable carbon source but is also a surface-active agent which may accelerate the oxygen transfer. This may lead to the possibility of the enhanced production of tetracycline at a low cost in airlift bioreactor.

  SOC BIOSCIENCE BIOENGINEERING JAPAN, Jun. 1999, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 87(6) (6), 825 - 827, English

  Scientific journal


• Kinetics of soybean oil consumption and cephamycin C production in culture of Streptomyces sp using mineral support

  EY Park, M Ichida, P Kahar, M Okabe

  A simple kinetics of soybean on consumption and cephamycin C production in Streptomyces sp. culture using a mineral support is proposed in this study. The mineral support was used for both suspending the soybean oil as fine oil droplets and immobilizing mycelia. The optimum concentrations of oil and mineral support for obtaining the maximum cephamycin C production were determined to be 50 and 15 g/l, respectively, by the proposed kinetics. At the optimal concentrations, the concentration of cephamycin C estimated from the proposed model and from the experimental data was 2.82 and 2.80 g/l respectively.,The results of the simulation coincided well with the experimental data for various concentrations of the soybean oil and the support. This demonstrates that our model can explain the kinetics of a culture using vegetable oil-as the carbon source and mineral support for both oil suspension and mycelial immobilization.

  SOC BIOSCIENCE BIOENGINEERING JAPAN, Mar. 1999, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 87(3) (3), 390 - 393, English

  Scientific journal


• Enhancement of yeast fermentation by addition of oxygen vectors in air-lift bioreactor

  S Jia, MX Wang, P Kahar, Y Park, M Okabe

  The effect of an oxygen vector on the oxygen transfer rate in air-lift bioreactor was evaluated using the ratio of the volumetric oxygen transfer coefficient to the volumetric fraction of the oxygen vector. When n-dodecane and perfluorocarbon were added at final concentrations of 3% and 2% (v/v), respectively, the oxygen transfer rate reached a maximum value. By addition of 3% (v/v) of n-dodecane to the yeast fermentation, the yeast concentration increased to 26.2 g/l which was 20% higher than in the case of fermentation in the absence of the oxygen vector.

  SOC FERMENTATION BIOENGINEERING, JAPAN, 1997, JOURNAL OF FERMENTATION AND BIOENGINEERING, 84(2) (2), 176 - 178, English

  Scientific journal

• Novel method for simple antibody modification on nanoparticle surfaces using Spytag-Spycatcher system

  松澤翼, 石田舞, 山手康輝, WINDA Tasia, PRIHARDI Kahar, 森裕太郎, 荻野千秋

  (公社)日本生物工学会, Aug. 2024, 日本生物工学会大会講演要旨集, 2024年, 83 - 83, Japanese


• Development of titanium peroxide nanoparticles using food ingredients and investigation of their cell-damaging effects

  山手康輝, 松澤翼, WINDA Tasia, PRIHARDI Kahar, 森裕太郎, 荻野千秋

  (公社)日本生物工学会, Aug. 2024, 日本生物工学会大会講演要旨集, 2024年, 83 - 83, Japanese


• Differences between hyphae-aggregated type and hyphae-dispersed type of Aspergillus oryzae based on observations of cell organelles

  鈴木智大, BAIHAQQI Fahmi, 松本琴音, 本迫翔, 若井暁, 若井暁, 森裕太郎, KAHAR Prihardi, 荻野千秋

  (公社)日本生物工学会, Aug. 2024, 日本生物工学会大会講演要旨集, 2024年, 94 - 94, Japanese


• 遺伝子組換え麹菌におけるイソプリメベロース生成酵素の産出に対する発酵パラメータの影響(Influence of fermentation parameter to the production of isoprimeverose-producing enzyme in genetically engineered Aspergillus oryzae)

  Baihaqqi Fahmi, Wakai Satoshi, Suzuki Tomohiro, Kahar Prihardi, Ogino Chiaki

  (公社)日本生物工学会, Aug. 2024, 日本生物工学会大会講演要旨集, 2024年, 94 - 94, English


• Culture characteristics of a filamentous fungus Aspergillus oryzae on different growth substrates including Sorghum juice

  本迫翔, 松本琴音, 鈴木智大, 若井暁, 若井暁, KAHAR Prihardi, 森裕太郎, 佐塚隆志, 荻野千秋

  (公社)日本生物工学会, Aug. 2024, 日本生物工学会大会講演要旨集, 2024年, 94 - 94, Japanese


• High cell density cultures of S. cerevisiae using an improved MAXBLEND reactor

  池谷佳朗, 田中彩, KAHAR Prihardi, 荻野千秋, 竹中克英

  (公社)日本生物工学会, Aug. 2024, 日本生物工学会大会講演要旨集, 2024年, 127 - 127, Japanese


• Construction and validation of a metabolic pathway model for thermophilic actinomycetes

  山田透瑚, PRIHARDI Kahar, 森祐太郎, 荻野千秋

  (公社)日本生物工学会, Aug. 2024, 日本生物工学会大会講演要旨集, 2024年, 130 - 130, Japanese


• Secretory production of PETase by Streptomyces thermoviolaceus and its characterization

  坂本大輔, 森裕太郎, PRIHARDI Kahar, 荻野千秋

  (公社)日本生物工学会, Aug. 2024, 日本生物工学会大会講演要旨集, 2024年, 130 - 130, Japanese


• Ralstonia eutrophaおよびその組換え株による種々の炭素源からのポリヒドロキシ酪酸(PHB)の生産(Production of poly-hydroxybutyrate(PHB) by Ralstonia eutropha and its recombinant strain from various carbon sources)

  Nadhifah Hana, Kano Atsuki, Chen Tzu-Yu, Kahar Prihardi, Mori Yutaro, Ogino Chiaki

  (公社)日本生物工学会, Aug. 2024, 日本生物工学会大会講演要旨集, 2024年, 194 - 194, English


• Effect of pyruvate flux engineering on protein production in Aspergillus oryzae

  松本琴音, 鈴木智大, 若井暁, 若井暁, PRIHARDI Kahar, 森裕太郎, 荻野千秋

  (公社)日本生物工学会, Aug. 2024, 日本生物工学会大会講演要旨集, 2024年, 246 - 246, Japanese


• Microbial production of lipids from various carbon sources by oleaginous yeast Lipomyces starkeyi

  石岡晃大, KAHAR Prihardi, 森裕太郎, 荻野千秋

  2024, 日本生物工学会大会講演要旨集, 76th


• Degradation and assimilation of Alcohol Maleate by esterase displaying yeast

  前橋咲希, KAHAR Prihardi, 森裕太郎, 荻野千秋

  2024, 日本生物工学会大会講演要旨集, 76th


• Current technologies for the utilization of grass biorefinery crops

  川口秀夫, KAHAR Prihardi, 荻野千秋

  2023, アグリバイオ, 7(6) (6)


• S.cerevisiae BA11の耐性評価とキシロース資化性付与

  樫谷侑太朗, 浅田元子, KAHAR Prihardi, 荻野千秋, 中村嘉利

  2023, 日本農芸化学会西日本支部大会およびシンポジウム講演要旨集, 2023 (CD-ROM)


• Characterization of cultures with Saccharomyces yeast using MAXBLEND reactor

  池谷佳朗, 堀口洋郎, 田中彩, KAHAR Prihardi, 荻野千秋, 竹中克英

  2023, 日本生物工学会大会講演要旨集, 75th


• Potential production of rare-disaccharide isoprimeverose by enzyme produced in Aspergillus oryzae

  BAIHAQQI Fhami, SUZUKI Tomohiro, WAKAI Satoshi, KAHAR Prihardi, KONDO Akihiko, OGINO Chiaki

  2023, 日本生物工学会大会講演要旨集, 75th


• Elucidation of radio sensitizing mechanism of hydrogen peroxide using genome editing cells

  鷲尾周, TASIA Winda, 山手康輝, 松澤翼, 森裕太郎, KAHAR Prihardi, 近藤昭彦, 荻野千秋

  2023, 日本生物工学会大会講演要旨集, 75th


• 酵母の個体差の解析を目的とした空圧バルブ付きマイクロ流体デバイスの開発

  藤原稜平, KAHAR Prihardi, 荻野千秋, 神野伊策, 肥田博隆

  2023, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 48th


• Production of Biodegradable Plastics from Palm Industrial Waste as a Carbon Source by the Oleaginous Yeast Lipomyces starkeyi

  中村優里, 壷井ひかり, KAHAR Prihardi, 近藤昭彦, 荻野千秋

  2022, 日本生物工学会大会講演要旨集, 74th


• Development of organic acid assimilating yeast and their application to bio-refineries from lignocellulosic biomass

  荻野千秋, KAHAR Prihardi, 壷井ひかり, LUCKI Risanto, 近藤昭彦

  2022, 日本生物工学会大会講演要旨集, 74th


• 外来タンパク質を提示したインフルエンザVLPsの昆虫細胞を用いた生産

  市川祐大, 松田拓也, PRIHARDI Kahar, 勝田知尚, 山地秀樹

  2022, Program & Abstracts. Annual and International Meeting of the Japanese Association for Animal Cell Technology, 35th (CD-ROM)


• 酵母の個別解析のためのバルブ付きマイクロ流体デバイスの開発

  矢郷望, KAHAR Prihardi, 荻野千秋, 神野伊策, 肥田博隆

  2022, 化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM), 46th


• Comparative analysis of metabolic strategies among various yeast species

  清家泰介, KAHAR Prihardi, 荻野千秋, 松田史生

  2022, 日本生物工学会大会講演要旨集, 74th


• 異種タンパク質を提示するインフルエンザウイルス様粒子の昆虫細胞を用いた生産

  市川祐大, 松田拓也, KAHAR Prihardi, 勝田知尚, 山地秀樹

  2022, 化学工学会年会研究発表講演要旨集(CD-ROM), 87th


• パーム油産業廃棄物POMEを原料とする微生物による酵素・化学品への変換

  荻野千秋, RACHMADORA Nova, KAHAR Prihardi, 近藤昭彦

  2021, 化学工学会年会研究発表講演要旨集(CD-ROM), 86th


• Breeding of organic acid assimilation yeast for bio-refinery

  壷井ひかり, KAHAR Prihardi, 近藤昭彦, 荻野千秋

  2021, 日本生物工学会大会講演要旨集, 73rd


• Polyhydroxyalkanoic acid production by an oleaginous yeast strain Lipomyces starkeyi

  中村優里, 壷井ひかり, KAHAR Prihardi, 近藤昭彦, 荻野千秋

  2021, 日本生物工学会大会講演要旨集, 73rd


• ゲノム編集技術を用いた遺伝子組換え昆虫細胞の樹立

  松田拓也, 戸上真也, 増見恭子, KAHAR Prihardi, 勝田知尚, 山地秀樹

  2021, Program & Abstracts. Annual and International Meeting of the Japanese Association for Animal Cell Technology, 34th


• 遺伝子組換え昆虫細胞樹立へのゲノム編集技術の応用

  松田拓也, 戸上真也, 増見恭子, KAHAR Prihardi, 勝田知尚, 山地秀樹

  2021, 化学工学会秋季大会研究発表講演要旨集(CD-ROM), 52nd


• 酵母カルチャーコレクションからのバイオエタノール生産のための優良酵母の選抜と評価

  荻野千秋, KAHAR Prihardi, 近藤昭彦

  2019, 日本生物工学会大会講演要旨集, 71st


• 油脂酵母Lipomyces starkeyiの遺伝子的改変による長鎖長脂肪酸の生産

  宮本捺央, LASTINOV Amza Rezky, KAHAR Prihardi, 荻野千秋, 近藤昭彦

  (公社)日本生物工学会, 2019, 日本生物工学会大会講演要旨集, 71st, 132 - 132, Japanese


• 油脂酵母Lipomyces starkeyiD35株の繰り返し発酵による脂肪酸組成変化解析

  宮本 捺央, Kahar Prihardi, 荻野 千秋, 近藤 昭彦

  (公社)日本生物工学会, Aug. 2018, 日本生物工学会大会講演要旨集, 平成30年度, 113 - 113, Japanese


• Saccharomyces cerevisiae酵母におけるCandida boidinii由来キシロース発酵代謝系の導入効果

  PRIHARDI Kahar, 紀平知枝, 大塚裕美, 荻野千秋, 近藤昭彦

  2018, 日本農芸化学会大会講演要旨集(Web), 2018


• The enhancement of culture conditions for high cell and lipid production of oleaginous yeast Lipomyces starkeyii D35

  AMZA Rezky Lastinov, KAHAR Prihardi, JUANSSILFERO Ario Betha, OTSUKA Hiromi, KIHIRA Chie, OGINO Chiaki, KONDO Akihiko

  2018, 日本生物工学会大会講演要旨集, 70th


• バイオマス分解酵素の大規模スクリーニングで見出した希少放線菌

  竹中武藏, LEE Jae Min, APRILIANA Pamella, 柏木紀賢, KAHAR Prihardi, 荻野千秋, 近藤昭彦

  2018, 日本生物工学会大会講演要旨集, 70th


• Exploration of potential indigenous marine actinomycetes from indonesia soil producing enzyme lignocellulosic for biorefinery application

  PAMELLA Apriliana, RAHMANI Nanik, IZZUDDIN Fahrurrozi, LISDIYANTI Puspita, LEE Jaemin, KAHAR Prihardi, YOPI, PRASETYA Bambang, OGINO Chiaki, KONDO Akihiko

  2017, 日本生物工学会大会講演要旨集, 69th


• The establishment high cell density culture of oleaginous Lipomyces starkeyi D35 for high cell and lipid production

  AMZA Rezky Lastinov, KAHAR Prihardi, JUANSSILFERO Ario Betha, JUANSSILFERO Ario Betha, OTSUKA Hiromi, KIHIRA Chie, OGINO Chiaki, KONDO Akihiko

  2017, 日本生物工学会大会講演要旨集, 69th


• High Production of single cell oil from glucose and xylose using oleaginous yeast Lipomyces starkeyi

  JUANSSILFERO Ario Betha, JUANSSILFERO Ario Betha, KAHAR Prihardi, AMZA Rezky Lastinov, OTSUKA Hiromi, MATSUMOTO Hana, KIHIRA Chie, THONTOWI Ahmad, YOPI, OGINO Chiaki, PRASETYA Bambang, KONDO Akihiko

  2017, 日本生物工学会大会講演要旨集, 69th


• 発酵阻害耐性酵母由来強力なENO1プロモーターの発現解析

  AZMI Nurlina B., KAHAR Prihardi, 糸見明穂, 紀平知枝, 荻野千秋, 近藤昭彦

  2017, 日本生物工学会大会講演要旨集, 69th


• 発酵阻害物質耐性Candida boidinii K212のキシロース発酵の解析

  PRIHARDI Kahar, 紀平知枝, 大塚裕美, 荻野千秋, 近藤昭彦, 近藤昭彦

  2017, 日本生物工学会大会講演要旨集, 69th


• From mannan to bioethanol: cell surface co-display of beta-mannanase and beta-mannosidase on yeast Saccharomyces cerevisiae

  Jun Ishii, Fumiyoshi Okazaki, Apridah Cameliawati Djohan, Kiyotaka Y. Hara, Nanami Asai-Nakashima, Hiroshi Teramura, Ade Andriani, Masahiro Tominaga, Satoshi Wakai, Prihardi Kahar, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo

  Background: Mannans represent the largest hemicellulosic fraction in softwoods and also serve as carbohydrate stores in various plants. However, the utilization of mannans as sustainable resources has been less advanced in sustainable biofuel development. Based on a yeast cell surface-display technology that enables the immobilization of multiple enzymes on the yeast cell walls, we constructed a recombinant Saccharomyces cerevisiae strain that co-displays beta-mannanase and beta-mannosidase; this strain is expected to facilitate ethanol fermentation using mannan as a biomass source. Results: Parental yeast S. cerevisiae assimilated mannose and glucose as monomeric sugars, producing ethanol from mannose. We constructed yeast strains that express tethered beta-mannanase and beta-mannosidase; co-display of the two enzymes on the cell surface was confirmed by immunofluorescence staining and enzyme activity assays. The constructed yeast cells successfully hydrolyzed 1,4-beta-D-mannan and produced ethanol by assimilating the resulting mannose without external addition of enzymes. Furthermore, the constructed strain produced ethanol from 1,4-beta-D-mannan continually during the third batch of repeated fermentation. Additionally, the constructed strain produced ethanol from ivory nut mannan; ethanol yield was improved by NaOH pretreatment of the substrate. Conclusions: We successfully displayed beta-mannanase and beta-mannosidase on the yeast cell surface. Our results clearly demonstrate the utility of the strain co-displaying beta-mannanase and beta-mannosidase for ethanol fermentation from mannan biomass. Thus, co-tethering beta-mannanase and beta-mannosidase on the yeast cell surface provides a powerful platform technology for yeast fermentation toward the production of bioethanol and other biochemicals from lignocellulosic materials containing mannan components.

  BIOMED CENTRAL LTD, Sep. 2016, BIOTECHNOLOGY FOR BIOFUELS, 9(Sept) (Sept), 9:188 (WEB ONLY), English

  Link to Kobe Univ. Repository (Kernel)


• 凝集性酵母における新規な阻害剤耐性機構の解明

  糸見明穂, 紀平和枝, KAHAR Prihardi, 荻野千秋, 近藤昭彦

  25 Aug. 2016, 日本生物工学会大会講演要旨集, 68th, 316, Japanese


• Yeast breeding for fuels and chemicals production from Indonesia Culture Collection

  KAHAR Prihardi, OTSUKA Hiromi, KIHARA Chie, ITOMI Akiho, LEE Jaemin, THONTOWI Ahmad, OCTAVIANA Senlio, JALU Filemon, KANTI Atit, YOPI, OGINO Chiaki, PRASETYA Bambang, KONDO Akihiko

  13 Mar. 2016, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N316, English


• Economic Value of Current Main Use of Oil Palm Empty Fruit Bunches (EFB) and Its Potential Use for Biofuel

  SIMAMORA Manaek, OGINO Chiaki, SUNARYA Yopi, KAHAR Prihardi, PRASETYA Bambang, PRASETYA Bambang, SUBIYANTO Bambang, YAMAN Aris, MAULANA Syafrizal, MALUDIN Syafrizal, MUNAWAR Sasa Sofyan

  13 Mar. 2016, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N321, English


• Biorefinery research in Kobe and Indonesia

  OGINO Chiaki, LEE JaeMin, KAHAR Prihardi, KONDO Akihiko

  13 Mar. 2016, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N302, English


• Biorefinery as strategic approach for supporting of utilization of tropical lignocellulosic biomas

  PRASETYA Bambang, SUNARYA Yopi, HERMIATI Euis, THONTOWI Ahmad, HANAFI, SIMAMORA Manaek, OGINO Chiaki, KAHAR Prihardi

  13 Mar. 2016, 化学工学会年会研究発表講演要旨集(CD-ROM), 81st, ROMBUNNO.N313, English


• マンナンバイオマスからのエタノール生産:β‐マンナナーゼとβ‐マンノシダーゼを細胞表層に提示した出芽酵母の開発

  石井純, 岡崎文美, DJOHAN Apridah Cameliawati, 原清敬, 浅井菜々実, ANDRIANI Ade, 寺村浩, KAHAR Prihardi, YOPI, PRASETYA Bambang, 荻野千秋, 近藤昭彦

  05 Mar. 2016, 日本農芸化学会大会講演要旨集(Web), 2016, 4A022 (WEB ONLY), Japanese


• インドネシアの土壌から分離された放線菌由来マンナナーゼ酵素の単離と酵素活性評価

  RAHMANI Nanik, 柏木紀賢, LEE JaeMin, 中村聡子, 松本華, KAHAR Prihardi, LISDIYANTI Puspita, YOPI, PRASETYA Bambang

  07 Sep. 2015, 日本放線菌学会大会講演要旨集, 30th, 105, Japanese


• 実バイオマスを微生物変換するための新しい酵母プラットフォームの探索

  KAHAR Prihardi, LEE Jaemin, 荻野千秋, 近藤昭彦

  05 Mar. 2015, 日本農芸化学会大会講演要旨集(Web), 2015, 3B33A01 (WEB ONLY), Japanese


• 実バイオマスからエタノール発酵するための酵母株の探索

  KAHAR Prihardi, 李載ミン, 松本華, 大塚裕美, 荻野千秋, 近藤昭彦

  2015, 日本ゲノム微生物学会年会要旨集, 9th


• インドネシアを例としたバイオコンビナート構想

  荻野千秋, KAHAR Prihardi, 李載みん, 近藤昭彦

  2015, 化学工学会大会講演要旨集(CD-ROM), 2015


• 2P-132 Production of low-temperature cellulase by Trichoderma reesei mutant

  Ookubo Shigeaki, Prihardi Kahar, Taku Kazuo, Tanaka Shuzo

  The Society for Biotechnology, Japan, 25 Aug. 2013, 日本生物工学会大会講演要旨集, 65, 137 - 137, Japanese


• キシロース発酵における細胞融合酵母FSC株のin vitro代謝解析

  KAHAR Prihardi, 田中修三

  2013, 日本農芸化学会大会講演要旨集(Web), 2013


• 4Ia05 Synergistic effect of delignification and alkaline swelling treatments on enzymatic degradation of rice straw

  Kahar Prihardi, Taku Kazuo, Tanaka Shuzo

  The Society for Biotechnology, Japan, 25 Sep. 2012, 日本生物工学会大会講演要旨集, 64, 240 - 240, English


• 稲わらの並行復発酵ための高活性セルラーゼ生産

  KAHAR Prihardi, 大久保成章, 多久和夫, 田中修三

  2012, 日本農芸化学会大会講演要旨集(Web), 2012


• 2Aa02 Xylose-fermenting yeast developed by mutation-fusion technique for bioethanol production

  KAHAR Prihardi, Shiroma Kazuki, Ohkubo Shigeaki, Taku Kazuo, Tanaka Shuzo

  The Society for Biotechnology, Japan, 25 Aug. 2011, 日本生物工学会大会講演要旨集, 63, 109 - 109, Japanese


• 高効率エタノール変換に向けた稲藁の脱リグニン及び弛緩処理技術の開発

  PRIHARDI Kahar, 多久和夫, 田中修三

  2011, 日本農芸化学会大会講演要旨集, 2011


• 2P-1185 Enhancement of xylose uptake in Saccharomyces cerevisiae by EMS mutagenesis

  Kahar Prihardi, Taku Kazuo, Tanaka Shuzo

  The Society for Biotechnology, Japan, 25 Sep. 2010, 日本生物工学会大会講演要旨集, 22, 55 - 55, Japanese


• 2Ha04 High efficient biomass conversion of unused agricultural residues to bioethanol

  Prihardi Kahar, Kumagai Ayaka, Taku Kazuo, Tanaka Shuzo

  The Society for Biotechnology, Japan, 25 Aug. 2009, 日本生物工学会大会講演要旨集, 21, 106 - 106, Japanese


• 1J14-5 Effect of PHA synthase activity on polyester molecular weight

  TSUGE Takeharu, AGUS Jumiarti, KAHAR Prihardi, ABE Hideki

  The Society for Biotechnology, Japan, 03 Aug. 2006, 日本生物工学会大会講演要旨集, 18, 176 - 176, Japanese


• 遺伝子組換え大腸菌が合成するポリ[(R)-3-ヒドロキシブタン酸]の分子量

  柘植丈治, AGUS Jumiarti, KAHAR Prihardi, 阿部英喜, 阿部英喜

  2006, 高分子学会予稿集(CD-ROM), 55(1 Disk1) (1 Disk1)


• INSIGHT INTO THE MOLECULAR ROTATION OF C-SUBUNIT RING IN THERMOPHILIC BACILLUS PS3 ATP SYNTHASE

  KAHAR Prihardi, SUZUKI Toshiharu, YOSHIDA Masasuke

  2006, 生化学


• 2P220 Insigh into The molecular rotation of c-subunit ring in Thermophilic Bacillus PS3 ATP synthase(37. Molecular motor (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

  Kahar Prihardi, Suzuki Toshiharu, Yoshida Masasuke

  The Biophysical Society of Japan General Incorporated Association, 2006, Seibutsu Butsuri, 46(2) (2), S350, English


• ポリ(3-ヒドロキシブチレート)の分子量における重合酵素の発現量および酵素活性の影響

  KAHAR Prihardi, KAHAR Prihardi, AGUS Jumiarti, 柘植丈治, 土肥義治, 土肥義治

  2005, 高分子学会予稿集(CD-ROM), 54(1 Disk1) (1 Disk1)


• Effect of temperature on molecular weight of poly[(R)-3-hydroxybutyrate)] synthesized by Bacillus sp. INT005 PHA synthase in recombinant Escherichia coli

  AGUS Jumiarti, KAHAR Prihardi, TSUGE Takeharu, ABE Hideki, ABE Hideki, DOI Yoshiharu

  2005, 高分子学会予稿集(CD-ROM), 54(2 Disk1) (2 Disk1)


• 2D14-1 Production of polyhydroxyalkanoate copolymer from soybean oil and its LCI analysis

  TSUGE Takeharu, KAHAR Prihardi, TAGUCHI Kazunori, AKIYAMA Minoru, DOI Yoshiharu

  The Society for Biotechnology, Japan, 25 Aug. 2004, 日本生物工学会大会講演要旨集, 16, 156 - 156, Japanese


• Factors affecting the molecular weight of poly[(R)-3-hydroxybutyrate] in recombinant Escherichia coli

  AGUS J, KAHAR P, TSUGE T, DOI Y

  2004, 高分子学会予稿集(CD-ROM), 53(2 Disk1) (2 Disk1)


• 遺伝子組換え大腸菌による超高分子量P(3HB)の効率的生産

  KAHAR P, 柘植丈治, 田口一徳, 土肥義治

  2004, 高分子学会予稿集(CD-ROM), 53(1) (1)


• 遺伝子組換え微生物を用いたポリヒドロキシアルカン酸(PHA)の効率的生合成手法の開発

  KAHAR P, AGUS J, 柘植丈治, 田口一徳, 土肥義治

  2004, 高分子学会予稿集(CD-ROM), 53(2 Disk1) (2 Disk1)


• 大豆油を原料としたバイオポリエステルの高効率・高収率生産

  KAHAR P, 柘植丈治, 田口一徳, 土肥義治

  2003, 高分子学会予稿集, 52(5) (5)


• バイオポリエステル微生物生産とライフサイクルインベントリ評価

  柘植丈治, KAHAR P, 秋山稔, 田口一徳, 土肥義治

  2003, 高分子学会予稿集, 52(14) (14)


• Enhancement of epsilon-polylysine production by Streptomyces albulus strain 410 using pH control

  P Kahar, T Iwata, J Hiraki, EY Park, M Okabe

  The enhancement of epsilon -poly-L-lysine (epsilon -PL) production by Streptomyces albulus strain no. 410 (S410) by means of a pH control strategy was investigated. S140 cells produce epsilon -PL at a high concentration if the culture pH remains at about 4.0; however, if it shifts to higher than 4.0, the accumulated epsilon -PL is depolymerized. We therefore suggest a pH control strategy for cell growth and epsilon -PL production aimed at increasing the amount of epsilon -PL produced. The cultivation was divided into two control phases. In phase I, cell growth was accelerated by maintaining the pH at higher than 5.0; in phase II, epsilon -PL production was increased by maintaining the pH at about 4.0. To avoid an increase in the pH during phase II as a result of glucose depletion, the glucose concentration was kept at around 10 g/l by glucose feeding. This control strategy enhanced the production of h-PL to 48.3 g/l from 5.7 g/l in the case of batch culture.

  SOC BIOSCIENCE BIOENGINEERING JAPAN, Feb. 2001, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 91(2) (2), 190 - 194, English


• 放線菌Streptomyces therotoleransによる基質タロシンからアセチルイソバレリルタイロシン(AIV)への微生物交換プロセスにおける基質流加速度の最適化

  黄国偉, 岡部満康, カハル プリハルディ, 朴龍しゅ, 恒川博

  01 Dec. 2000, 日本農芸化学会誌, 74(12) (12), 1399, Japanese


• Effect of Soybean Oil on Production of Antibiotics

  Kahar Prihardi, Akashi Kazunori, Siru Jia, Park Enoch Y, Okabe Mitsuyasu

  The Society for Biotechnology, Japan, 16 Aug. 1999, 日本生物工学会大会講演要旨集, 11, 228 - 228, Japanese


• 1029 3-アセチル-4"-イソバレリルタイロシン(AIV)生産における動力学モデルの作成

  黄 国偉, PRIHARDI KAHAR, 朴 龍洙, 岡部 満康

  公益社団法人日本生物工学会, 16 Aug. 1999, 日本生物工学会大会講演要旨集, 11, 225 - 225, Japanese


• STUDY ON THE MICROBIAL PRODUCTION OF ε-POLYLYSINE IN AN AIRLIFT BIOREACTOR

  Kahar Prihardi, Park Enoch. Y, Okabe Mitsuyasu

  The Society for Biotechnology, Japan, 16 Aug. 1999, 日本生物工学会大会講演要旨集, 11, 218 - 218, Japanese


• Study on Enhanced Microbial Production of εPL by Streptomyces albulus No.410

  Kahar Prihardi, Iwata Toshiharu, Hiraki Jun, Park Yong Soo, Okabe Mitsuyasu

  The Society for Biotechnology, Japan, 31 Aug. 1998, 日本生物工学会大会講演要旨集, 10, 215 - 215, Japanese

• The Palm Oil Export Market: Trends, Challenges, and Future Strategies for Sustainability (Routledge Studies in the Economics of Business and Industry)

  Prihardi Kahar, Noor-Afiqah Ahmad Zain, Radityo Pangestu, Chiaki Ogino

  Joint work, Important key points regarding the circular bioeconomy in the palm oil industry, Routledge, Mar. 2025, ISBN: 103285541X


• Handbook of Biorefinery Research and Technology

  Prihardi Kahar, Gregory Guirimand, Tomohisa Hasunuma

  Ethanol Production by Recombinant CBP Yeasts, Springer, Dordrecht, Nov. 2024, ISBN: 9400763077


• Advanced Lignin Technologies

  Filemon Jalu, Nusantara Putra, Prihardi Kahar, Chiaki Ogino, Akihiko Kondo

  Joint work, Advanced Lignin Valorization for Biorefinery Application, Intechopen, Jul. 2024, ISBN: 0854667644


• Current technologies for the utilization of grass biorefinery crops

  Hideo Kawaguchi, Prihardi Kahar, Chiaki Ogino

  Joint work, Agriculturak Biotechnology, Jun. 2023


• Environmental Biotechnology: New Approaches and Prospective Applications

  Prihardi Kahar

  Single work, Synergistic Effects of Pretreatment Process on Enzymatic Digestion of Rice Straw for Efficient Ethanol Fermentation, Intechopen, Feb. 2013, ISBN: 9789535109723

• REDOXバランスにおける発酵阻害耐性酵母由来新規TDHxの影響

  Azmi Nurlina, 糸見 明穂, PRIHARDI KAHAR, OGINO Chiaki, KONDO Akihiko

  第19回化学工学会学生発表会, Mar. 2017, Japanese, 化学工学会関西支部, 豊中市, Domestic conference

  Oral presentation


• Development of Platform Yeast Strain Capable of Direct Fermentation of Raw Biomass to Ethanol

  PRIHARDI KAHAR, Akiho Itomi, Ahmad Thontowi, Hiromi Otsuka, Chie Kihira, Jaemin Lee, Ario Betha Juanssil fero, Apridah Cameliawati Djohan, Yopi Sunarya, Ishii Jun, OGINO Chiaki, Banbang Prasetya, KONDO Akihiko

  Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conference

  Oral presentation


• Yeast breeding for fuels and chemicals production from Indonesia Culture Collection

  KAHAR Prihardi, OTSUKA Hiromi, KIHARA Chie, ITOMI Akiho, LEE Jaemin, THONTOWI Ahmad, OCTAVIANA Senlio, JALU Filemon, KANTI Atit, YOPI, OGINO Chiaki, PRASETYA Bambang, KONDO Akihiko

  化学工学会年会研究発表講演要旨集(CD-ROM), Mar. 2016, English


• Economic Value of Current Main Use of Oil Palm Empty Fruit Bunches (EFB) and Its Potential Use for Biofuel

  SIMAMORA Manaek, OGINO Chiaki, SUNARYA Yopi, KAHAR Prihardi, PRASETYA Bambang, PRASETYA Bambang, SUBIYANTO Bambang, YAMAN Aris, MAULANA Syafrizal, MALUDIN Syafrizal, MUNAWAR Sasa Sofyan

  化学工学会年会研究発表講演要旨集(CD-ROM), Mar. 2016, English


• Biorefinery research in Kobe and Indonesia

  OGINO Chiaki, LEE JaeMin, KAHAR Prihardi, KONDO Akihiko

  化学工学会年会研究発表講演要旨集(CD-ROM), Mar. 2016, English


• Biorefinery as strategic approach for supporting of utilization of tropical lignocellulosic biomas

  PRASETYA Bambang, SUNARYA Yopi, HERMIATI Euis, THONTOWI Ahmad, HANAFI, SIMAMORA Manaek, OGINO Chiaki, KAHAR Prihardi

  化学工学会年会研究発表講演要旨集(CD-ROM), Mar. 2016, English


• マンナンバイオマスからのエタノール生産：βｰマンナナーゼとβｰマンノシダーゼを細胞表層に提示した出芽酵母の開発

  OGINO CHIAKI, ISHII JUN, OKAZAKI FUMIYOSHI, DJHAN APRIDAH CAMELIWATI, HARA KIYOTAKA, ASAI NANAMI, ANDORIAN ADE, TERAMURA HIROSHI, KAHAR PRIHARDI, YOPI, PRASETYA BAMBANG

  日本農芸化学会2016年度大会, Mar. 2016, Japanese, Domestic conference

  Oral presentation


• インドネシアの土壌から分離された放線菌由来マンナナーゼ酵素の単離と酵素活性評価

  RAHMANI Nanik, 柏木紀賢, LEE JaeMin, 中村聡子, 松本華, KAHAR Prihardi, LISDIYANTI Puspita, YOPI, PRASETYA Bambang

  日本放線菌学会大会講演要旨集, Sep. 2015, Japanese


• 実バイオマスを微生物変換するための新しい酵母プラットフォームの探索

  KAHAR Prihardi, LEE Jaemin, 荻野千秋, 近藤昭彦

  日本農芸化学会大会講演要旨集(Web), Mar. 2015, Japanese


• 実バイオマスを微生物変換するための新しい酵母プラットフォームの探索

  PRIHARDI KAHAR, 李 載みん, OGINO CHIAKI, KONDO AKIHIKO

  日本農芸化学会2015年度大会, Mar. 2015, Japanese, 日本農芸化学会, 岡山市, Domestic conference

  Oral presentation


• 実バイオマスからエタノール発酵するための酵母株の探索

  KAHAR Prihardi, 李載ミン, 松本華, 大塚裕美, 荻野千秋, 近藤昭彦

  日本ゲノム微生物学会年会要旨集, 2015, Japanese


• インドネシアを例としたバイオコンビナート構想

  荻野千秋, KAHAR Prihardi, 李載みん, 近藤昭彦

  化学工学会大会講演要旨集(CD-ROM), 2015, Japanese


• Trichoderma reesei変異菌による低温性セルラーゼの産生

  大久保成章, KAHAR Prihardi, 多久和夫, 田中修三

  日本生物工学会大会講演要旨集, Aug. 2013, Japanese


• キシロース発酵における細胞融合酵母FSC株のin vitro代謝解析

  KAHAR Prihardi, 田中修三

  日本農芸化学会大会講演要旨集(Web), Mar. 2013, Japanese


• Synergistic effect of delignification and alkaline swelling treatments on enzymatic degradation of rice straw

  KAHAR PRIHARDI, TAKU KAZUO, TANAKA SHUZO

  日本生物工学会大会講演要旨集, Sep. 2012, English


• 稲わらの並行復発酵ための高活性セルラーゼ生産

  KAHAR Prihardi, 大久保成章, 多久和夫, 田中修三

  日本農芸化学会大会講演要旨集(Web), Mar. 2012, Japanese


• バイオエタノール生産ための細胞変異融合法によるキシロース代謝利用酵母の分子育種

  KAHAR Prihardi, 城間一樹, 大久保成章, 多久和夫, 田中修三

  日本生物工学会大会講演要旨集, Aug. 2011, Japanese


• 高効率エタノール変換に向けた稲藁の脱リグニン及び弛緩処理技術の開発

  PRIHARDI Kahar, 多久和夫, 田中修三

  日本農芸化学会大会講演要旨集, Mar. 2011, Japanese


• EMS変処理によるサカロマイセス酵母のキシロース取込能力の強化

  PRIHARDI Kahar, 多久和夫, 田中修三

  日本生物工学会大会講演要旨集, Sep. 2010, Japanese


• 未利用農業廃棄物からバイオエタノールへの効率的変換

  PRIHARDI Kahar, 熊谷綾華, 多久和夫, 田中修三

  日本生物工学会大会講演要旨集, Aug. 2009, Japanese


• 微生物合成ポリエステルの分子量特性

  柘植丈治, 冨澤哲, AGUS Jumiarti, KAHAR Priharudi, 阿部英喜, 田口精一

  高分子学会予稿集(CD-ROM), Sep. 2007, Japanese


• Insigh into The molecular rotation of c-subunit ring in Thermophilic Bacillus PS3 ATP synthase

  KAHAR PRIHARDI, SUZUKI TOSHIHARU, YOSHIDA MASASUKE

  生物物理, Oct. 2006, English


• 2P220 Insigh into The molecular rotation of c-subunit ring in Thermophilic Bacillus PS3 ATP synthase(37. Molecular motor (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

  Kahar Prihardi, Suzuki Toshiharu, Yoshida Masasuke

  Biophysics, Oct. 2006, English


• PHA合成酵素活性がポリ[(R)‐3‐ヒドロキシブタン酸]の分子量に及ぼす影響

  柘植丈治, AGUS Jumiarti, KAHAR Prihardi, 阿部英喜

  日本生物工学会大会講演要旨集, Aug. 2006, Japanese


• 遺伝子組換え大腸菌が合成するポリ[(R)‐3‐ヒドロキシブタン酸]の分子量

  柘植丈治, AGUS Jumiarti, KAHAR Prihardi, 阿部英喜

  高分子学会予稿集(CD-ROM), May 2006, Japanese


• INSIGHT INTO THE MOLECULAR ROTATION OF C-SUBUNIT RING IN THERMOPHILIC BACILLUS PS3 ATP SYNTHASE

  KAHAR PRIHARDI, SUZUKI TOSHIHARU, YOSHIDA MASASUKE

  生化学, 2006, English


• Molecular weight of poly[(R)-3-hydroxybutyrate] synthesized by type IV PHA synthase in Escherichia coli

  Jumiarti Agus, Jumiarti Agus, Prihardi Kahar, Prihardi Kahar, Takeharu Tsuge, Takeharu Tsuge, Yoshiharu Doi, Yoshiharu Doi

  Polymer Preprints, Japan, Dec. 2005, In this study, we investigated the molecular weight of P(3HB) synthesized by the type IV PHA synthase from Bacillus sp. INT005 (PhaRC Bs). The PhaRC Bs preferred to synthesize low molecular weight of P(3HB) in Escherichia coli. The curve of molecular weight distribution was found to depend on cultivation time. Host strain of E. coli also affected the molecular weight of polymer, but culture temperature did not.


• Effect of temperature on molecular weight of poly[(R)-3-hydroxybutyrate)] synthesized by Bacillus sp. INT005 PHA synthase in recombinant Escherichia coli

  AGUS JUMIARTI, KAHAR PRIHARDI, TSUGE TAKEHARU, ABE HIDEKI, DOI YOSHIHARU

  高分子学会予稿集(CD-ROM), Sep. 2005, English


• ポリ(3‐ヒドロキシブチレート)の分子量における重合酵素の発現量および酵素活性の影響

  KAHAR Prihardi, AGUS Jumiarti, 柘植丈治, 土肥義治

  高分子学会予稿集(CD-ROM), May 2005, Japanese


• Molecular weight of poly[(R)-3-hydroxybutyrate] synthesized by type IV PHA synthase in Escherichia coli

  AGUS JUMIARTI, KAHAR PRIHARDI, TSUGE TAKEHARU, DOI YOSHIHARU

  高分子学会予稿集(CD-ROM), May 2005, English


• 遺伝子組換え微生物を用いたポリヒドロキシアルカン酸(PHA)の効率的生合成手法の開発

  KAHAR P, AGUS J, 柘植丈治, 田口一徳, 土肥義治

  高分子学会予稿集(CD-ROM), Sep. 2004, Japanese


• Factors affecting the molecular weight of poly[(R)-3-hydroxybutyrate] in recombinant Escherichia coli

  AGUS J, KAHAR P, TSUGE T, DOI Y

  高分子学会予稿集(CD-ROM), Sep. 2004, English


• 大豆油からのバイオポリエステル生産と環境影響評価

  柘植丈治, KAHAR Prihardi, 田口一徳, 秋山稔, 土肥義治

  日本生物工学会大会講演要旨集, Aug. 2004, Japanese


• 遺伝子組換え大腸菌による超高分子量P(3HB)の効率的生産

  KAHAR P, 柘植丈治, 田口一徳, 土肥義治

  高分子学会予稿集(CD-ROM), May 2004, Japanese


• 大豆油からのバイオポリエステル生産と環境影響評価

  TAKEHARU TSUGE, PRIHARDI KAHAR, KAZUNORI TAGUCHI, MINORU AKIYAMA, YOSHIHARU DOI

  第56回日本生物工学会大会, 2004, Japanese, 日本生物工学会, 名城大学、名古屋市, Domestic conference

  Oral presentation


• Effective Production and Kinetic Characteristic of Ultra-high-molecular-weight of PHB in Recombinant Escherichia coli.

  PRIHARDI KAHAR, TAKEHARU TSUGE, KAZUNORI TAGUCHI, YOSHIHARU DOI

  Int. Symposium on Biological Polyesters, 2004, English, IUPAC, Beijing, China, International conference

  Poster presentation


• バイオポリエステル微生物生産とライフサイクルインベントリ評価

  柘植丈治, KAHAR P, 秋山稔, 田口一徳, 土肥義治

  高分子学会予稿集, Sep. 2003, Japanese


• 大豆油を原料としたバイオポリエステルの高効率・高収率生産

  KAHAR P, 柘植丈治, 田口一徳, 土肥義治

  高分子学会予稿集, May 2003, Japanese


• エアリフト型バイオリアクター(Airlift Bioreactor,ABR)におけるε‐ポリリジン(ε‐Polylysine,ε‐PL)の生産に関する研究

  KAHAR P, 岡部満康, 小島麻美, 小林健吾, 平木純, 岩田敏治

  日本農芸化学会大会講演要旨集, Mar. 2002, Japanese


• 有限要素法による気泡塔における溶存酸素分布の解析

  PRIHARDI KAHAR, 小島麻美, 岡部満康

  乳酸菌工学研究部会講演会, 2002, Japanese, 日本生物工学会, 茨城, Domestic conference

  [Invited]

  Invited oral presentation


• 放線菌Streptomyces therotoleransによる基質タロシンからアセチルイソバレリルタイロシン(AIV)への微生物交換プロセスにおける基質流加速度の最適化

  黄国偉, 岡部満康, カハル プリハルディ, 朴龍しゅ, 恒川博

  日本農芸化学会誌, Dec. 2000, Japanese


• エアリフト型バイオリアクターを用いた培養系における溶存酸素濃度の時間的分布の解析

  KAHAR P, 小島麻美, 朴龍しゅ, 岡部満康

  化学工学会秋季大会研究発表講演要旨集, Aug. 2000, Japanese


• Production of epsilon-polylysine by Streptomyces albulus Strain.

  PRIHARDI KAHAR, ENOCH Y. PARK, MITSUYASU OKABE

  The 11th International Biotechnology Symposium, 2000, English, IUPAC, Berlin, Germany, International conference

  Poster presentation


• 抗生物質生産への大豆油の効果について

  KAHAR P, 明石和憲, SIRU J, PARK E Y, 岡部満康

  日本生物工学会大会講演要旨集, Aug. 1999, Japanese


• エアリフト型バイオリアクターでのε‐ポリリジン(PL)の発酵生産に関する研究

  KAHAR P, PARK E Y, OKABE M

  日本生物工学会大会講演要旨集, Aug. 1999, Japanese


• 3‐アセチル‐4′′‐イソバレリルタイロシン(AIV)生産における動力学モデルの成

  黄国偉, PRIHARDI K, 朴龍しゅ, 岡部満康

  日本生物工学会大会講演要旨集, Aug. 1999, Japanese


• Effect of Soybean Oil on Production of Antibiotics

  Kahar Prihardi, Akashi Kazunori, Siru Jia, Park Enoch Y, Okabe Mitsuyasu

  日本生物工学会大会講演要旨集, Aug. 1999, Japanese


• 1029 3-アセチル-4"-イソバレリルタイロシン(AIV)生産における動力学モデルの作成

  黄 国偉, PRIHARDI KAHAR, 朴 龍洙, 岡部 満康

  日本生物工学会大会講演要旨集, Aug. 1999, Japanese


• STUDY ON THE MICROBIAL PRODUCTION OF ε-POLYLYSINE IN AN AIRLIFT BIOREACTOR

  Kahar Prihardi, Park Enoch. Y, Okabe Mitsuyasu

  日本生物工学会大会講演要旨集, Aug. 1999, Japanese


• Study on Enhanced Microbial Production of εPL by Streptomyces albulus No.410

  Kahar Prihardi, Iwata Toshiharu, Hiraki Jun, Park Yong Soo, Okabe Mitsuyasu

  日本生物工学会大会講演要旨集, Aug. 1998, Japanese


• Study on Enhanced Microbial Production of .EPSILON.PL by Streptomyces albulus No.410.

  KAHAR P, IWATA TOSHIHARU, HIRAKI JUN, PARK Y S, OKABE MITSUYASU

  日本生物工学会大会講演要旨集, 1998, Japanese

• 日本化学工学会

  Jan. 2023 - Present


• 日本高分子学会

  Apr. 2002 - Present


• 日本農芸化学会

  Apr. 1996 - Present


• 日本生物工学会

  Apr. 1996 - Present

• Production and Diversification of High Purity Poly(lactic acid) based Polymer by Robust Yeast

  Prihardi Kahar

  Fujimori Science and Technology Foundation, Research Grand, Apr. 2023 - Mar. 2024, Principal investigator


• 実バイオマス発酵に適した高性能キシロース資化酵母の新規プラットフォームの開発

  KAHAR PRIHARDI

  学術研究助成基金助成金／基盤研究(C), Apr. 2016 - Mar. 2019, Principal investigator

  Competitive research funding

• ポリ－３－ヒドロキシアルカン酸の製造方法

  土肥義治, 柘植丈治, 田口一徳, プリハルディ・カハル

  特願2004-100098, 30 Mar. 2004, 特開2005-278559, 13 Oct. 2005

  Patent right

• Editorial Board Member

  Editorial Board Member

  Frontiers in Bioengineering and Biotechnology

  01 Mar. 2023 - Present

  Peer review etc


• Editorial Board Member

  Editorial Board Member

  SynBio

  01 Jan. 2022 - Present

  Peer review etc


• Editorial Board Member

  Editorial Board Member

  Processes journal

  01 Apr. 2021 - Present

  Peer review etc