Research activity information

• Nov. 2014 細菌学会関西支部総会, 若手研究者奨励賞

  青木千恵

• Statins enhance extracellular release of hepatitis C virus particles through ERK5 activation.

  Chie Aoki-Utsubo, Masanori Kameoka, Lin Deng, Muhammad Hanafi, Beti Ernawati Dewi, Pratiwi Sudarmono, Takaji Wakita, Hak Hotta

  Statins, such as lovastatin, have been known to inhibit 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase. Statins were reported to moderately suppress hepatitis C virus (HCV) replication in cultured cells harboring HCV RNA replicons. We report here using an HCV cell culture (HCVcc) system that high concentrations of lovastatin (5-20 μg/mL) markedly enhanced the release of HCV infectious particles (virion) in the culture supernatants by up to 40 times, without enhancing HCV RNA replication, HCV protein synthesis, or HCV virion assembly in the cells. We also found that lovastatin increased the phosphorylation (activation) level of extracellular-signal-regulated kinase 5 (ERK5) in both the infected and uninfected cells in a dose-dependent manner. The lovastatin-mediated increase of HCV virion release was partially reversed by selective ERK5 inhibitors, BIX02189 and XMD8-92, or by ERK5 knockdown using small interfering RNA (siRNA). Moreover, we demonstrated that other cholesterol-lowering statins, but not dehydrolovastatin that is incapable of inhibiting HMG-CoA reductase and activating ERK5, enhanced HCV virion release to the same extent as observed with lovastatin. These results collectively suggest that statins markedly enhance HCV virion release from infected cells through HMG-CoA reductase inhibition and ERK5 activation.

  Jul. 2024, Microbiology and immunology, English, International magazine

  [Refereed]

  Scientific journal


• Construction of Fosmid-based SARS-CoV-2 replicons for antiviral drug screening and replication analyses in biosafety level 2 facilities.

  Shunta Takazawa, Tomohiro Kotaki, Satsuki Nakamura, Chie Utsubo, Masanori Kameoka

  The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has necessitated the global development of countermeasures since its outbreak. However, current therapeutics and vaccines to stop the pandemic are insufficient and this is mainly because of the emergence of resistant variants, which requires the urgent development of new countermeasures, such as antiviral drugs. Replicons, self-replicating RNAs that do not produce virions, are a promising system for this purpose because they safely recreate viral replication, enabling antiviral screening in biosafety level (BSL)-2 facilities. We herein constructed three pCC2Fos-based RNA replicons lacking some open reading frames (ORF) of SARS-CoV-2: the Δorf2-8, Δorf2.4, and Δorf2 replicons, and validated their replication in Huh-7 cells. The functionalities of the Δorf2-8 and Δorf2.4 replicons for antiviral drug screening were also confirmed. We conducted puromycin selection following the construction of the Δorf2.4-puro replicon by inserting a puromycin-resistant gene into the Δorf2.4 replicon. We observed the more sustained replication of the Δorf2.4-puro replicon by puromycin pressure. The present results will contribute to the establishment of a safe and useful replicon system for analyzing SARS-CoV-2 replication mechanisms as well as the development of novel antiviral drugs in BSL-2 facilities.

  Sep. 2023, Virus research, 334, 199176 - 199176, English, International magazine

  Scientific journal


• Identification of an Oligostilbene, Vaticanol B, from Dryobalanops aromatica Leaves as an Antiviral Compound against Hepatitis C Virus

  Chie Aoki-Utsubo, Muhammad Hanafi, Destia Tri Armanti, Hiroyuki Fuchino, Nobuo Kawahara, Sri Hartati, Aty Widyawaruyanti, Pratiwi Sudarmono, Masanori Kameoka, Hak Hotta

  Chronic hepatitis C virus (HCV) infection can lead to liver cirrhosis and hepatocellular carcinoma. Although current medications using direct-acting antivirals (DAAs) are highly effective and well-tolerated for treating patients with chronic HCV, high prices and the existence of DAA-resistant variants hamper treatment. There is thus a need for easily accessible antivirals with different mechanisms of action. During the screening of Indonesian medicinal plants for anti-HCV activity, we found that a crude extract of Dryobalanops aromatica leaves possessed strong antiviral activity against HCV. Bioassay-guided purification identified an oligostilbene, vaticanol B, as an active compound responsible for the anti-HCV activity. Vaticanol B inhibited HCV infection in a dose-dependent manner with 50% effective and cytotoxic concentrations of 3.6 and 559.5 µg/mL, respectively (Selectivity Index: 155.4). A time-of-addition study revealed that the infectivity of HCV virions was largely lost upon vaticanol B pretreatment. Also, the addition of vaticanol B following viral entry slightly but significantly suppressed HCV replication and HCV protein expression in HCV-infected and a subgenomic HCV replicon cells. Thus, the results clearly demonstrated that vaticanol B acted mainly on the viral entry step, while acting weakly on the post-entry step as well. Furthermore, co-treatment of the HCV NS5A inhibitor daclatasvir with vaticanol B increased the anti-HCV effect. Collectively, the present study has identified a plant-derived oligostilbene, vaticanol B, as a novel anti-HCV compound.

  Aug. 2023, Biol. Pharm. Bull., 46(8) (8), 1079 - 1087, English, Domestic magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Amentoflavone inhibits hepatitis B virus infection via the suppression of preS1 binding to host cells.

  Chie Aoki-Utsubo, Puguh Indrasetiawan, Kento Fukano, Masamichi Muramatsu, Nina Artanti, Muhammad Hanafi, Hak Hotta, Masanori Kameoka

  Hepatitis B virus (HBV) is a leading cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Current therapeutic drugs for chronic HBV infection use IFN and nucleos(t)ide analogs; however, their efficacy is limited. Thus, there is an urgent need to develop new antivirals for HBV therapy. In this study, we identified a plant-derived polyphenolic bioflavonoid, amentoflavone, as a new anti-HBV compound. Amentoflavone treatment dose-dependently inhibited HBV infection in HBV-susceptible cells with HepG2-hNTCP-C4 and primary human hepatocyte PXB-cells. A mode-of-action study showed that amentoflavone inhibits the viral entry step, but not the viral internalization and early replication processes. Attachment of HBV particles as well as HBV preS1 peptide to HepG2-hNTCP-C4 cells was inhibited by amentoflavone. The transporter assay revealed that amentoflavone partly inhibits uptake of sodium taurocholate cotransporting polypeptide (NTCP)-mediated bile acid. Furthermore, effect of various amentoflavone analogs on HBs and HBe production from HBV-infected HepG2-hNTCP-C4 cells was examined. Robustaflavone exhibited comparable anti-HBV activity to that of amentoflavone and an amentoflavone-7,4', 4‴-trimethyl ether derivative (sciadopitysin) with moderate anti-HBV activity. Cupressuflavone or the monomeric flavonoid apigenin did not exhibit the antiviral activity. Amentoflavone and its structurally related biflavonoids may provide a potential drug scaffold in the design of a new anti-HBV drug inhibitor targeting NTCP.

  Mar. 2023, Microbiology and immunology, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Acacia mangium: A promising plant for isolating anti-hepatitis C virus agents

  Tutik Sri Wahyuni, Nida S. Sukma, Adita A. Permanasari, Chie Aoki-Utsubo, Aty Widyawaruyanti, Achmad Fuad Hafid

  Background: Medicinal plants have been demonstrated to possess various pharmacological effects including anti-hepatitis C virus. Acacia  mangium is one of the Acacia genus that contain with  various metabolites. The metaboilites play an  important role for antiviral activities. The current study examined the anti-hepatitis C virus (HCV) activities of Acacia mangium extracts in solvents with various polarities and further evaluated the mechanism of action of the extracts on the protein virus and combination treatment models.

  Methods: Anti-hepatitis C virus activities was conducted by in vitro culture cells of Huh 7it both in a single or combination treatment. Further examined its NS3 protein inhibition was evaluated by western blotting assay.

  Results: The results revealed the strong anti-HCV activities of the extracts. The 50% inhibition concentrations (IC₅₀s) of the ethanol, n-hexane, dichloromethane and methanol extracts were of 4.6 ± 0.3, 2.9 ± 0.2, 0.2 ± 0.3, and 2.8 ± 0.2 μg/mL, respectively, and no cytotoxic effect was detected. These extracts displayed stronger effects than the positive control ribavirin. The mode of action of the ethanol extract was evaluated at 30 µg/mL, revealing that the inhibitory effect was stronger on the post-entry step than on the entry step. Western blotting revealed that the extracts decreased NS3 protein expression, indicating that virus replication was suppressed. Further evaluation illustrated that combined treatment with the ethanol extract enhanced the anti-viral activity of simeprevir.

  Conclusions: These results indicated that A. mangium leaves could represent sources of anti-HCV agents.

  F1000 Research Ltd, Feb. 2023, F1000Research, 11, 1452 - 1452

  Scientific journal


• An in vitro study of an Artocarpus heterophyllus substance as a hepatitis C antiviral and its combination with current anti-HCV drugs

  Adita Ayu Permanasari, Chie Aoki-Utsubo, Tutik Sri Wahyuni, Lidya Tumewu, Myrna Adianti, Aty Widyawaruyanti, Hak Hotta, Achmad Fuad Hafid

  Current therapy of chronic hepatitis C virus (HCV) with direct-acting antivirals (DAAs) has dramatically improved the sustained virologic response (SVR) of affected patients; however, treatment with DAAs remains expensive, and drug-resistant HCV variants remain a threat. As a result, there is still a need to continue to develop affordable and effective drugs for the treatment of HCV. Previously, we have demonstrated that a crude extract from Artocarpus heterophyllus leaves is a potential anti-HCV candidate. In this study, we have further purified this crude extract, examined which sub-fraction possesses the highest antiviral activity, and then explored its efficacy at different HCV life cycle stages. We also assessed synergistic antiviral effects between the A. heterophyllus extract and commercially available anti-HCV drugs. We used vacuum liquid chromatography (VLC) and high-performance liquid chromatography (HPLC) to fractionate a dichloromethane extract of A. heterophyllus leaves. We then examined the anti-HCV activity of the fractions using HCV genotype 2a, JFH1a; the antiviral mode of action was determined by exploring adding the treatments at different times. We examined the antiviral effects on the viral entry stage through a virucidal activity test, viral adsorption examination, and pretreatment of cells with the drug. The effects on the post-viral entry stage were determined by the levels of HCV protein expression and HCV RNA expression in infected cells. Through activity guided purification, we identified the sub-fraction FR3T3 as possessing the most robust anti-HCV activity with an IC₅₀ value of 4.7 ± 1.0 μg/mL. Mode-of-action analysis revealed that FR3T3 inhibited post-viral entry stages such as HCV NS3 protein expression and HCV RNA replication with marginal effects on the viral entry stage. Thin-layer Chromatography (TLC) indicated that FR3T3 contained terpenoids and chlorophyll-related compounds. We also found a synergistic antiviral activity when the DCM extract of A. heterohyllus was used in combination therapy with commercial anti-HCV drugs; Ribavirin, Simeprevir, Cyclosporin A. The extract of A. heterophyllus and its sub-fraction, FR3T3, presented here have anti-HCV activities and could be candidate drugs for add-on-therapy for treatment of chronic HCV infections.

  Springer Science and Business Media LLC, Dec. 2021, BMC Complementary Medicine and Therapies, 21(1) (1)

  Scientific journal


• Alkaloid and benzopyran compounds of Melicope latifolia fruit exhibit anti-hepatitis C virus activities

  Aty Widyawaruyanti, Mulyadi Tanjung, Adita Ayu Permanasari, Ratih Saputri, Lidya Tumewu, Myrna Adianti, Chie Aoki-Utsubo, Hak Hotta, Achmad Fuad Hafid, Tutik Sri Wahyuni

  New agents for developing alternative or complementary medicine to treat the hepatitis C virus (HCV) are still needed due to high rates of HCV infection globally and the current limitations of available treatments. Treatment of HCV with a combination of direct acting antivirals have been shown to be approximately 90% effective but will be limited in the future due to the emergence of drug resistance and high cost. The leaves of Melicope latifolia have previously been reported to have anti-HCV activity and are a potential source of bioactive compounds for future novel drug development. This study aimed to evaluate the efficacy of the extract of M. latifolia fruit to treat HCV and to isolate its active compounds. M. latifolia fruit was extracted using methanol and purified using vacuum liquid chromatography (VLC) and Radial Chromatography. The anti-HCV activity was analyzed using cell culture lines Huh7it-1 and JFH1 (genotype 2a). Time-of-addition and immunoblotting studies were performed to identify the mode of action of the isolated active compounds. The structures of the active compounds were determined using nuclear magnetic resonance (NMR) spectra, UV, IR, and Mass Spectra. Six known compounds were isolated from M. latifolia fruit: O-methyloktadrenolon, alloevodionol, isopimpinellin, alloxanthoxyletin, methylevodionol, and N-methylflindersine. N-methylflidersine was the most active compound with IC₅₀ value of 3.8 μg/ml while methylevodionol, isopimpinellin, and alloevodionol were less active. O-methyloktadrenolon and alloxanthoxyletin were moderately active with IC₅₀ values of 10.9 and 21.72 μg/ml, respectively. N-methylflidersine decreased level of HCV NS3 protein expression in the cells. The alkaloid compound, N-methylflindersine which was isolated from M. latifolia possesses anti-HCV activity through post-entry inhibition and suppressed NS3 protein expression.

  Springer Science and Business Media LLC, Dec. 2021, BMC Complementary Medicine and Therapies, 21(1) (1)

  Scientific journal


• Antiviral activity of Indonesian Medicinal Plants against hepatitis B virus.

  Wahyuni TS, Permanasari A, Widyawaruyanti A, Hotta H, Chie AU, Hafid A

  2020, Pharmacognosy Journal, 12(5) (5), 1108 - 1114


• Smp76, a Scorpine-Like Peptide Isolated from the Venom of the Scorpion Scorpio maurus palmatus, with a Potent Antiviral Activity Against Hepatitis C Virus and Dengue Virus

  Alaa M.H. El-Bitar, Moustafa Sarhan, Mohamed A. Abdel-Rahman, Veronica Quintero-Hernandez, Chie Aoki-Utsubo, Mohsen A. Moustafa, Lourival D. Possani, Hak Hotta

  Springer Science and Business Media LLC, Jan. 2019, International Journal of Peptide Research and Therapeutics, 26(2) (2), 811 - 821

  Scientific journal


• Antiviral Activity of Cananga odorata Against Hepatitis B

  Indrasetiawan P, Aoki-Utsubo C, Hanafi M, Hartati S, Wahyuni TS, Kameoka M, Hotta H, Hayashi Y

  Chronic hepatitis B virus (HBV) infection can lead to liver cirrhosis and hepatocellular carcinoma. Current therapeutic drugs for chronic hepatitis B using pegylated interferons and nucleos(t)ide analogs have limited efficacy. Therefore, the development of novel and safe antivirals is required. Natural products including medicinal plants produce complex and structurally diverse compounds, some of which offer suitable targets for antiviral screening studies. In the present study, we screened various crude extracts from Indonesian plants for anti-HBV activity by determining their effects on the production of extracellular HBV DNA in Hep38.7-Tet cells and HBV entry onto a HBV-susceptible cell line, HepG2-NTCP, with the following results: (1) In Hep38.7-Tet cells, Cananga odorata exhibited the highest anti-HBV activity with a 50% inhibitory concentration (IC50) of 56.5 µg/ml and 50% cytotoxic concentration (CC50) of 540.2 µg/ml (Selectivity Index: 9.6). (2) The treatment of HepG2-NTCP cells with Cassia fistula, C. odorata, and Melastoma malabathricum at concentrations of 100 µg/ml lowered the levels of HBsAg production to 51.2%, 58.0%, and 40.1%, respectively, compared to untreated controls, and IC50 and CC50 values of C. odorata were 142.9 µg/ml and >400 µg/ml. In conclusion, the C. odorata extract could be a good candidate for the development of anti-HBV drugs.

  Corresponding, 2019, Kobe Journal of Medical Sciences, 65(2) (2), E71 - E79, English, Domestic magazine

  [Refereed]

  Scientific journal


• Antiviral Activities of Curcuma Genus against Hepatitis C Virus

  Tutik Sri Wahyuni, Adita Ayu Permatasari, Tri Widiandani, Achmad Fuad, Aty Widyawaruyanti, Chie Aoki-Utsubo, Hak Hotta

  Dec. 2018, NATURAL PRODUCT COMMUNICATIONS, 13(12) (12), 1579 - 1582

  [Refereed]


• Potential of Indonesian Plants (Annona muricata, Garcinia latissima, and Garcinia celebica) Against Hepatitis C Virus

  Apriyanto Dadan Ramadhan, Aoki-Utsubo Chie, Sri Hartati, Dewi Beti Ernawati, Hotta Hak

  Sep. 2018, ADVANCED SCIENCE LETTERS, 24(9) (9), 6807 - 6810

  [Refereed]


• Threonine-Allyl-Ester and Amide of Gallic Acid Derivative as Promising Anti-HCV Inhibitors

  Apriyanto Dadan Ramadhan, Arsianti Ade, Aoki-Utsubo Chie, Fadilah, Bahtiar Anton, Dwira Surya, Paramita Rafika Indah, Hotta Hak

  Aug. 2018, ADVANCED SCIENCE LETTERS, 24(8) (8), 6265 - 6267

  [Refereed]


• Virucidal and Neutralizing Activity Tests for Antiviral Substances and Antibodies

  Aoki-Utsubo Chie, Chen Ming, Hotta Hak

  Lead, May 2018, BIO-PROTOCOL, 8(10) (10)

  [Refereed]

  Scientific journal


• Time-of-addition and Temperature-shift Assays to Determine Particular Step(s) in the Viral Life Cycle that is Blocked by Antiviral Substance(s)

  Aoki-Utsubo Chie, Chen Ming, Hotta Hak

  Lead, May 2018, BIO-PROTOCOL, 8(9) (9)

  [Refereed]


• Antiviral effect of archidendron pauciflorum leaves extract to hepatitis C virus: An in vitro study in JFH-1 strain

  Sri Hartati, Chie Aoki, Muhammad Hanafi, Marissa Angelina, Pratiwi Soedarmono, Hak Hotta

  Mar. 2018, Medical Journal of Indonesia, 27(1) (1), 12 - 18, English

  [Refereed]

  Scientific journal


• Broad-spectrum antiviral agents: secreted phospholipase A(2) targets viral envelope lipid bilayers derived from the endoplasmic reticulum membrane

  Ming Chen, Chie Aoki-Utsubo, Masanori Kameoka, Lin Deng, Yutaka Terada, Wataru Kamitani, Kei Sato, Yoshio Koyanagi, Makoto Hijikata, Keiko Shindo, Takeshi Noda, Michinori Kohara, Hak Hotta

  Nov. 2017, SCIENTIFIC REPORTS, 7(1) (1), 15931, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Antiviral activity of the dichloromethane extracts from Artocarpus heterophyllus leaves against hepatitis C virus

  Achmad Fuad Hafid, Chie Aoki-Utsubo, Adita Ayu Permanasari, Myrna Adianti, Lydia Tumewu, Aty Widyawaruyanti, Sri Puji Astuti Wahyuningsih, Tutik Sri Wahyuni, Maria Inge Lusida, Soetjipto, Hak Hotta

  Jul. 2017, Asian Pacific Journal of Tropical Biomedicine, 7(7) (7), 633 - 639, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Synthesis and anti-hepatitis C virus activity of gallic acid derivatives with chiral center

  Ade A, Aoki-Utsubo C, Fadilah F, Anton B, Dadan RA, Surya D, Novia AP, Hiroyuki T, Kiyomi K, Pratiwi S, Hak H, Rakia IP, Linda E

  2017, Asian Journal of Pharmaceutical and Clinical Research, 10(7) (7), 164 - 167, English

  [Refereed]


• Anti-Hepatitis C Virus Activity of a Crude Extract from Longan (Dimocarpus longan Lour.) Leaves

  Dadan Ramadhan Apriyanto, Chie Aoki, Sri Hartati, Muhammad Hanafi, Leonardus Broto Sugeng Kardono, Ade Arsianti, Melva Louisa, Tjahjani Mirawati Sudiro, Beti Ernawati Dewi, Pratiwi Sudarmono, Amin Soebandrio, Hak Hotta

  Corresponding, May 2016, JAPANESE JOURNAL OF INFECTIOUS DISEASES, 69(3) (3), 213 - 220, English

  [Refereed]

  Scientific journal


• AntiHepatitis C Virus Activity of Alectryon serratus Leaves Extract

  Lidya Tumewu, Evhy Apryani, Mei Ria Santi, Tutik Sri Wahyuni, Adita Ayu Permanasari, Myrna Adianti, Chie Aoki, Aty Widyawaruyanti, Achmad Fuad Hafid, Maria Inge Lusida, Soetjipto, Hak Hotta

  2016, MOLECULAR AND CELLULAR LIFE SCIENCES: INFECTIOUS DISEASES, BIOCHEMISTRY AND STRUCTURAL BIOLOGY 2015 CONFERENCE, 18, 169 - 173, English

  [Refereed]

  International conference proceedings


• Activities of Ficus fistulosa Leave Extract and Fractions Against Hepatitis C Virus

  Achmad Fuad Hafid, Adita Ayu Permanasari, Lidya Tumewu, Myrna Adianti, Chie Aoki, Aty Widyawaruyanti, Soetjipto, Maria Inge Lusida, Hak Hotta

  2016, MOLECULAR AND CELLULAR LIFE SCIENCES: INFECTIOUS DISEASES, BIOCHEMISTRY AND STRUCTURAL BIOLOGY 2015 CONFERENCE, 18, 179 - 184, English

  [Refereed]

  International conference proceedings


• Virocidal activity of Egyptian scorpion venoms against hepatitis C virus

  Alaa M. H. El-Bitar, Moustafa M. H. Sarhan, Chie Aoki, Yusuke Takahara, Mari Komoto, Lin Deng, Mohsen A. Moustafa, Hak Hotta

  Mar. 2015, VIROLOGY JOURNAL, 12(1) (1), 47, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Inhibition of hepatitis C virus replication by chalepin and pseudane IX isolated from Ruta angustifolia leaves

  Tutik Sri Wahyuni, Aty Widyawaruyanti, Maria Inge Lusida, Achmad Fuad, Soetjipto, Hiroyuki Fuchino, Nobuo Kawahara, Yoshitake Hayashi, Chie Aoki, Hak Hotta

  Dec. 2014, FITOTERAPIA, 99, 276 - 283, English

  [Refereed]

  Scientific journal


• Induction of Cell-Mediated Immune Responses in Mice by DNA Vaccines That Express Hepatitis C Virus NS3 Mutants Lacking Serine Protease and NTPase/RNA Helicase Activities

  Suratno Lulut Ratnoglik, Da-Peng Jiang, Chie Aoki, Pratiwi Sudarmono, Ikuo Shoji, Lin Deng, Hak Hotta

  Jun. 2014, PLOS ONE, 9(6) (6), e98877, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Anti-hepatitis C virus compounds obtained from Glycyrrhiza uralensis and other Glycyrrhiza species

  Myrna Adianti, Chie Aoki, Mari Komoto, Lin Deng, Ikuo Shoji, Tutik Sri Wahyuni, Maria Inge Lusida, Soetjipto, Hiroyuki Fuchino, Nobuo Kawahara, Hak Hotta

  Mar. 2014, MICROBIOLOGY AND IMMUNOLOGY, 58(3) (3), 180 - 187, English

  [Refereed]

  Scientific journal


• Antiviral activity of extracts from Morinda citrifolia leaves and chlorophyll catabolites, pheophorbide a and pyropheophorbide a, against hepatitis C virus

  Suratno Lulut Ratnoglik, Chie Aoki, Pratiwi Sudarmono, Mari Komoto, Lin Deng, Ikuo Shoji, Hiroyuki Fuchino, Nobuo Kawahara, Hak Hotta

  Mar. 2014, MICROBIOLOGY AND IMMUNOLOGY, 58(3) (3), 188 - 194, English

  [Refereed]

  Scientific journal


• Isolation and identification of substances with anti-hepatitis c virus activities from kalanchoe pinnata

  Chie Aoki, Sri Hartati, Mei Ria Santi, Lydwina, Rininta Firdaus, Muhammad Hanafi, Leonardus B.S. Kardono, Yohko Shimizu, Pratiwi Sudarmono, Hak Hotta

  Lead, Jan. 2014, International Journal of Pharmacy and Pharmaceutical Sciences, 6, 211 - 215

  [Refereed]


• Antiviral activities of Indonesian medicinal plants in the East Java region against hepatitis C virus

  Tutik Sri Wahyuni, Lydia Tumewu, Adita Ayu Permanasari, Evhy Apriani, Myrna Adianti, Abdul Rahman, Aty Widyawaruyanti, Maria Inge Lusida, Achmad Fuad, Soetjipto, Nasronudin, Hiroyuki Fuchino, Nobuo Kawahara, Ikuo Shoji, Lin Deng, Chie Aoki, Hak Hotta

  Aug. 2013, VIROLOGY JOURNAL, 10, 259, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A point mutation at Asn-534 that disrupts a conserved N-glycosylation motif of the E2 glycoprotein of hepatitis C virus markedly enhances the sensitivity to antibody neutralization

  Mikiko Sasayama, Ikuo Shoji, Myrna Adianti, Da-Peng Jiang, Lin Deng, Takafumi Saito, Hisayoshi Watanabe, Sumio Kawata, Chie Aoki, Hak Hotta

  Feb. 2012, JOURNAL OF MEDICAL VIROLOGY, 84(2) (2), 229 - 234, English

  [Refereed]

  Scientific journal


• Generation of a recombinant reporter hepatitis C virus useful for the analyses of virus entry, intra-cellular replication and virion production

  Kazuya Kamada, Ikuo Shoji, Lin Deng, Chie Aoki, Suratno Lulut Ratnoglik, Takaji Wakita, Hak Hotta

  Jan. 2012, MICROBES AND INFECTION, 14(1) (1), 69 - 78, English

  [Refereed]

  Scientific journal


• Dengue virus type 2 recognizes the carbohydrate moiety of neutral glycosphingolipids in mammalian and mosquito cells（共著）

  Wichit S, Jittmittraphap A, Hidari KI, Thaisomboonsuk B, Petmitr S, Ubol S, Aoki C, Itonori S, Morita K, Suzuki T, Suzuki Y, Jampangern W

  日本細菌学会，日本ウイルス学会, Feb. 2011, Microbiol Immunol., 55(2) (2), 135 - 140, English

  [Refereed]

  Scientific journal


• Dengue virus type 2 recognizes the carbohydrate moiety of neutral glycosphingolipids in mammalian and mosquito cells

  Sineewanlaya Wichit, Akanitt Jittmittraphap, Kazuya I. P. J. Hidari, Butsaya Thaisomboonsuk, Songsak Petmitr, Sukathida Ubol, Chie Aoki, Saki Itonori, Koichi Morita, Takashi Suzuki, Yasuo Suzuki, Wipawee Jampangern

  Feb. 2011, MICROBIOLOGY AND IMMUNOLOGY, 55(2) (2), 135 - 140, English

  [Refereed]

  Scientific journal


• Development of a simple system for screening anti-hepatitis C virus drugs utilizing mutants capable of vigorous replication

  Lijuan Yu, Chie Aoki, Yohko Shimizu, Kazufumi Shimizu, Wei Hou, Fumihiro Yagyu, Xianzi Wen, Masamichi Oshima, Aikichi Iwamoto, Bin Gao, Wenjun Liu, George Fu Gao, Yoshihiro Kitamura

  Nov. 2010, JOURNAL OF VIROLOGICAL METHODS, 169(2) (2), 380 - 384, English

  Scientific journal


• A recombinant replication-competent hepatitis C virus expressing Azami-Green, a bright green-emitting fluorescent protein, suitable for visualization of infected cells

  Wei Hou, Chie Aoki, Lijuan Yu, Xianzi Wen, Yinhuan Xue, Bin Gao, Wenjun Liu, George Fu Gao, Aikichi Iwamoto, Yoshihiro Kitamura

  Dec. 2008, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 377(1) (1), 7 - 11, English

  [Refereed]

  Scientific journal


• Identification and characterization of carbohydrate molecules in mammalian cells recognized by dengue virus type 2

  C Aoki, KIPJ Hidari, S Itonori, A Yamada, N Takahashi, T Kasama, F Hasebe, MA Islam, K Hatano, K Matsuoka, T Taki, CT Guo, T Takahashi, Y Sakano, T Suzuki, D Miyamoto, M Sugita, D Terunuma, K Morita, Y Suzuki

  Mar. 2006, JOURNAL OF BIOCHEMISTRY, 139(3) (3), 607 - 614, English

  [Refereed]

  Scientific journal

• Antiviral Activity of Toad Cake and Its Compounds Bufalin and Gamabufotalin against Hepatitis C Virus and Dengue Virus

  堀田博, 靭千恵, 西本幸子, 増井涼, 杉本智潮, 清水康晴, 須藤慶一, 河上仁美, 渕野裕之, 川原信夫, 川原信夫

  2022, 甲南女子大学研究紀要2, (16) (16)


• Hepatitis B virus cccDNA generation is enhanced by the approved anti-tumor drug Mitoxantrone

  堀田博, 堀田博, 西本幸子, とう琳, 靭千恵, 勝二郁夫, 脇田隆字, 村松正道

  2021, 日本ウイルス学会学術集会プログラム・予稿集(Web), 68th


• A biflavonid amentoflavone inhibits hepatitis B virus infection

  靱千恵, INDRASETIAWAN Puguh, 深野顕人, 村松正道, 堀田博, 堀田博, 亀岡正典

  2021, 日本ウイルス学会学術集会プログラム・予稿集(Web), 68th


• Promising anti-hepatitis C virus compounds from natural resources

  Wahyuni TS, Aoki Utsubo C, HOTTA HAK

  2016, Natural Product Communications, 11(8) (8), 1193 - 1200, English

  [Refereed][Invited]

  Introduction scientific journal


• Syntheses and Biological Assay of a Series of Lacto-N-neotetraose Cluster using Carbosilane Dendrimer Scaffolds

  Akihiro, Yamada, Ken Hatano, Koji Matsuoka, Yasuaki Esumi, Chie Aoki, Kazuya Hidari, Yasuo Suzuki, Daiyo Terunuma

  2005, The 14th International Symposium on Organosilicon Chemistry, 134


• Isolation and characterization of glycolipids recognized with dengue virus type 2 from human and mosquito cells

  KIPJ Hidari, C Aoki, T Ogi, S Itonori, F Hasebe, K Morita, M Sugita, T Takahashi, CT Guo, D Miyamoto, T Suzuki, Y Suzuki

  Nov. 2004, GLYCOBIOLOGY, 14(11) (11), 1158 - 1158, English

  Summary international conference

• The multiplex CRISPR/Cas9 system efficiently suppresses HBV gene expression

  Izumi Tanaka, Risako Miki, Chie Utsubo, Masanori Kameoka

  第71回日本ウイルス学会, Nov. 2024


• Anti-hepatitis B and hepatitis C virus activity of butyrolactone I.

  Chie Aoki-Utsubo, Dewi Triana Rizna, Muhammad Hanafi, Hak Hotta, Masanori Kameoka

  第71回日本ウイルス学会, Nov. 2024


• SARS-CoV-2感染におけるサイトカインストーム誘導機構に対する脂質の関与

  梶川 美紗乃, 靱 千恵, 青野 友美, 亀岡 正典

  第70回日本ウイルス学会, Nov. 2023


• 新型コロナウイルスは肝細胞でアポリポプロテインを利用して増殖する

  靱千恵, 梶原美紗乃, 青野友美, 亀岡正典

  第70回日本ウイルス学会, Nov. 2023


• 新型コロナウイルスは肝細胞でアポリポプロテインBを利用して増殖する

  靱千恵, 梶川美紗乃, 青野友美, 亀岡正典

  日本薬学会第142年会, Mar. 2023

  Oral presentation


• 硫酸単糖型抗デングウイルス薬の合成研究(3)

  日本薬学

  日本薬学会第142年会, Mar. 2023


• A biflavonid amentoflavone inhibits hepatitis B virus infection

  靱 千恵, drasetiawan Puguh, 深野 顕人, 村松 正道, 堀田 博, 亀岡 正典

  第68回日本ウイルス学会学術集会, Nov. 2021, Japanese

  Poster presentation


• A biflavonoid amentoflavone inhibits hepatitis B virus infection

  Chie AOKI-UTSUBO, Indrasetiawan PUGUH, Kento FUKANO, Masamichi MURAMATSU, Masanori KAMEOKA, Hak HOTTA

  The 11th JSP-CSP-KSP Joint Symposium of Pharmacognosy, Sep. 2021, English

  Poster presentation


• Methyl-pheophorbide A and porphyrin derivatives inhibit the viral assembly step of hepatitis C virus

  Chie Aoki-Utsubo, Masanori Kameoka, Hak Hotta

  HCV2021: The International Symposium on Hepatitis C Virus and Related Viruses, Jul. 2021, English

  Poster presentation


• 新規デングウイルス感染阻害剤の合成（Ⅷ）

  佐藤 理貴、寺岡 文照、靭 千恵、左 一八、大坪 忠宗、池田 潔

  日本薬学会第140年会(京都), Mar. 2020


• 改変型糖鎖誘導体ライブラリーによるデングウイルス感染阻害効果の検討

  靱 千恵、寺岡 文照、大坪 忠宗、池田 潔、左 一八

  日本薬学会第140年会(京都), Mar. 2020


• A chlorophyll derivative, methyl-pheophorbide A inhibits hepatitis C virus assembly by affecting apolipoprotein production

  Chie Aoki-Utsubo, Hak Hotta

  第67回日本ウイルス学会学術集会, Oct. 2019, English, Domestic conference


• Antiviral Activity of Steroidal Cardiac Glycosides Obtained from Dried Toad Cake against Hepatitis C Virus

  Hak Hotta, Chie Aoki-Utsubo, Sachiko Nishimoto, Hiroyuki Fuchino, Nobuo Kawahara, Ryo Masui, Chishio Sugimoto, Yasuharu Shimizu, Keiichi Sudo

  第67回日本ウイルス学会学術集会, Oct. 2019, English, Domestic conference


• Further analysis of possible antiviral activity of CM-II-sPLA2 against HBV, HCV and HDV

  Hak Hotta, Chie Aoki-Utsubo, Ming Chen, Sachiko Nishimoto, Lin Deng, Yohei Miyayama, Makoto Hijikata, Keiko Shindo, Takeshi Noda, Michinori Kohara, Senko Tsukuda, Koichi Watashi, Takaji Wakita

  HBV2019 (International HBV Meeting), Oct. 2019, English, International conference


• Potent Anti-HCV Activity of Bufalin and Gamabufotalin Obtained from Dried Toad Cake

  Hak Hotta, Chie Aoki-Utsubo, Sachiko Nishimoto, Hiroyuki Fuchino, Nobuo Kawahara, Ryo Masui, Chishio Sugimoto, Yasuharu Shimizu, Keiichi Sudo

  International Meeting on HCV and Related Viruses 2019, Oct. 2019, English, International conference


• インドネシア原産薬用植物の抗B型肝炎ウイルス活性について

  靱千恵, Puguh Indrasetiawan, Yan Mardiani, Muhammad Hanafi, Sri Hartati, Tutik Sri Wahyuni, 亀岡正典, 矢野嘉彦, 堀田博, 林祥剛

  日本生薬学会第66年会, Sep. 2019, Japanese, Domestic conference


• Methyl-pheophorbide AによるC型肝炎ウイルス感染性粒子形成阻害機構の解析

  靱千恵, 堀田博

  第139年回 日本薬学会, Mar. 2019, Japanese, Domestic conference


• A crude extract from Cananga odorata exhibits antiviral activity against hepatitis B virus.

  Puguh Indrasetiawan, Chie Aoki-Utsubo, Muhammad Hanafi, Sri Hartati, Masanori Kameoka, Hak Hotta, Yoshitake Hayashi

  第139年回 日本薬学会, Mar. 2019, English, Domestic conference


• フッ素基導入型デングウイルス感染阻害剤の合成

  向原大貴, 寺岡文照, 靱千恵, 左一八, 大坪忠宗, 池田潔

  第139年会 日本薬学会, Mar. 2019, Japanese, Domestic conference


• 動物生薬の抗HCV活性について

  渕野裕之, 村瀬明香, 河上仁美, 川原信夫, 堀田博, 靭千恵, 増井涼, 杉本智潮, 清水康晴, 須藤慶一

  第139年会 日本薬学会, Mar. 2019, Japanese, Domestic conference


• Supporting evidence that the ER/ERGIC is the main budding site for the Hepatitis B virus virion

  陳明, 鄧琳, 靱千恵, 渡士幸一, 脇田隆字, 堀田博

  第63回日本ウイルス会学術集会, Oct. 2018, English, Domestic conference


• Mechanistical study of antiviral activity of snake venom sPLA2 against HBV and HCV

  Miyayama Yohei, Chen Ming, Aoki-Utsubo Chie, Deng Lin, Shido Keiko, Noda Takeshi, Kohara Michinori, Watashi Koichi, Wakita Takaji, Hijikata Makoto, Hotta Hak

  第64回日本ウイルス会学術集会, Oct. 2018, English, Domestic conference


• Hepatitis C virus infection and screening research of potential antiviral agents from natural products

  Chie Aoki-Utsubo

  International Course for Health Sciences Summer Educational Program, Sep. 2018, English

  Public discourse


• Comparative analysis of antiviral activity of sPLA2 against HBV and HCV

  Hotta H, Chen M, Aoki-Utsubo C, Deng L, Miyayama Y, Hijikata M, Shido K, Noda T, Kohara M, Watashi K, Wakita T

  2018 HBV International meeting, 2018, English, International conference


• 新規デングウイルス感染阻害剤の合成(VIII)

  寺岡文照, 左一八, 靱千恵, 大坪忠宗, 池田潔

  日本薬学会第138年会, 2018, Japanese, Domestic conference


• 化合物ライブラリーを用いた抗デングウイルス活性を示す化合物の探索

  上野稔, 靱千恵, 小瀧将裕, 亀岡正典

  第25回トガ•フラビ•ペスチウイルス研究会, 2018, Japanese, Domestic conference

  Oral presentation


• 含フッ素デングウイルス感染阻害剤の合成研究

  寺岡文照, 向原大貴, 左一八, 靱千恵, 大坪忠宗, 池田潔

  第57回日本薬学会中国四国支部第学術大会, 2018, Japanese, Domestic conference

  Oral presentation


• Chlorophyll-breakdown product, methyl-pheophorbide A inhibits hepatitis C virus infection by suppressing virus assembly

  靱千恵, Muhammad Hanafi, Pratiwi Sudarmono, 清水洋子, 堀田博

  第63回日本ウイルス会学術集会, Oct. 2017, English, Domestic conference

  Poster presentation


• Dryobalanops aromatica 葉部からのオリゴスチルベノイド、vaticanol B は C 型 肝炎ウイルスの感染を阻害する

  UTSUBO CHIE, Hanafi M, Widyamawaruyanti A, Armanti TD, 渕野裕之, 川原信夫, Sudarmono P, HOTTA HAK

  日本薬学会第137年会, Mar. 2017, Japanese, 仙台, Domestic conference

  Poster presentation


• Supporting evidence that the ER/ERGIC is the main budding site for the Hepatitis B virus virion

  Chen M, Deng L, Aoki-U C, Watashi K, Wakita T, Hotta H

  2017 HBV International meeting, 2017, English, International conference


• Broad-spectrum Antiviral Phospholipase A2 (PLA2) That Targets the Viral Envelope Lipid Bilayer derived from the ER/ERGIC Membranes

  Hotta H, Chen M, Aoki-U C, Deng L, Miyayama Y, Hijikata M, Shido K, Noda T, Kohara M, Watashi K, Wakita T

  24th International Symposium on Hepatitis C Virus and Related Viruses, 2017, English, International conference


• Antiviral activities of the scorpine-like peptide Smp 76 isolated from Scorpio maurus palmatus against dengue virus and hapatitis C virus.

  HOTTA HAK, El-Bitar AA, Sarhan MM, Rahman MA, Possani LD, Chen M, Deng L, Utsubo CA

  23th International Symposium on Hepatitis C Virus and Related Viruses, Oct. 2016, English, Kyoto, International conference

  Poster presentation


• Cholesterol-lowering statins enhance HCV virion release from infected cells through activation of ERK5

  Chie Aoki, Muhamma Hanafi, Leonardus B.S. Kardono, Beti Dewi, Pratiwi Sudarmono, Yohko Shimizu, Takaji Wakita, Hak Hotta

  22th International Symposium on Hepatitis C Virus and Related Viruses, Oct. 2015, English, International conference

  Poster presentation


• Lovastatin enhances HCV virion release through activation of ERK5.

  Chie Aoki, Muhammad Hanafi, Leonardus Kardono, Pratiwi Sudarmono, Yohko Shimizu, Takaji Wakita, Hak Hotta

  第63回日本ウイルス会学術集会, Oct. 2015, English, Domestic conference

  Oral presentation


• Inhibition of hepatitis C virus replication by Chalepin and Pseudane IX isolated from Ruta angustifolia leaves.

  Wahyuni TS, Widyawaruyanti A, Lusida MI, Fuad A, Soetjipto, Fuchino H, Kawahara N, Hayashi Yoshitake, Aoki Chie, Hotta Hak

  日本薬学会第135年会, Mar. 2015, English, 日本薬学会, 神戸, Domestic conference

  Oral presentation


• Aspergillus terreus からの精製物質ロバスタチンはC型肝炎ウイルス感染性粒子放出を促進する.

  Aoki Chie, Muhanmad Hanafi, Leonardus B.S. Kardono, 清水洋子, Pratiwi Sudarmono, Hotta Hak

  日本薬学会第135年会, Mar. 2015, Japanese, 日本薬学会, 神戸, Domestic conference

  Oral presentation


• C型肝炎ウイルス感染によるTGF-βスーパーファミリーにおけるSmad2/3とSmad1/5/9経路の脱制御とその分子機序の解明.

  松岡陽子, Deng Lin, 朝日朱美, Aoki Chie, Shoji Ikuo, Hotta Hak

  第62回日本ウイルス学会学術集会., Nov. 2014, Japanese, 日本ウイルス学会, 横浜, Domestic conference

  Oral presentation


• Aspergillus terreus 抽出液及びその精製物ロバスタチンは高濃度でC型肝炎ウイルス感染性粒子の放出を促進する.

  Aoki Chie, Muhanmad Hanafi, Leonardus B.S. Kardono, 清水洋子, Pratiwi Sudarmono, Hotta Hak

  第67回日本細菌学会関西支部総会学術集会, Nov. 2014, Japanese, 日本細菌学会関西支部, 西宮, Domestic conference

  Oral presentation


• HCV dysregulates Smad2/3- and Smad1/5-signaling pathways of the TGF-β superfamily.

  Yoko Matsuoka, Deng Lin, Akemi Asahi, Aoki Chie, Shoji Ikuo, Hotta Hak

  21th International Symposium on Hepatitis C Virus and Related Viruses., Sep. 2014, English, Organising Committee of the 21th International Symposium on Hepatitis C Virus and Related Viruses, Banff, Banff, International conference

  Poster presentation


• Chalepin and pseudane IX isolated from Ruta angustifolia leaves inhibit hepatitis C virus replication.

  Wahyuni TS, Widyawaruyanti A, Lusida MI, Fuad A, Soetjipto, Fuchino H, Kawahara N, Hayashi Yoshitake, Aoki Chie, Hotta Hak

  The 13th Awaji International Forum on Infection and Immunity in Nara., Sep. 2014, English, The Awaji International Forum on Infection and Immunity, Nara, International conference

  Poster presentation


• Chlorophill分解産物Pheophorbide a、Chlorin e6及び半合成誘導体Mono-L-aspartyl chlorin e6 (NPe6) はC型肝炎ウイルス増殖を阻害する

  Suratno Lulut Ratnoglik, Aoki Chie, 河本 真理, Pratiwi Sudarmono, Deng Lin, Shoji Ikuo, 渕野 裕之, 川原 信夫, Hotta Hak

  第61回日本ウイルス学会学術集会, Nov. 2013, Japanese, 日本ウイルス学会, 神戸, Domestic conference

  Poster presentation


• Aspergillus terreus 抽出液及びそれに含まれるロバスタチンは高濃度でC型肝炎ウイルス感染性粒子産生を促進する

  Aoki Chie, Shimizu Yoko, Sudarmono Pratiwi, Muhanmad Hanafi, Kardono B.S. Leonardus, Hotta Hak

  第61回日本ウイルス学会学術集会, Nov. 2013, Japanese, 日本ウイルス学会, 神戸, Domestic conference

  Oral presentation


• Various statins at high concentrations enhance HCV virion release from the infected cells

  Aoki Chie, Sri Hartati, Muhanmad Hanafi, Leonardus B.S. Kardono, Tjahjani Mirawati Sudiro, Beti Ernawati Dewi, Pratiwi Sudarmono, Shimizu Yoko, Hotta Hak

  20th International Symposium on Hepatitis C Virus and Related Viruses., Oct. 2013, English, Organising Committee of the20th International Symposium on Hepatitis C Virus and Related Viruses, Melbourne, Australia, International conference

  Poster presentation


• Development of a prophylactic and therapeutic vaccine against Hepatitis C virus

  Suratno Lulut Ratnoglik, Jiang Dapeng, Aoki Chie, Pratiwi Sudarmono, Deng Lin, Shoji Ikuo, Hotta Hak

  20th International Symposium on Hepatitis C Virus and Related Viruses., Oct. 2013, English, Organising Committee of the20th International Symposium on Hepatitis C Virus and Related Viruses, Melbourne, Australia, International conference

  Poster presentation


• Antiviral activity of chlorophyll derivatives, pheophorbide a, chlorin e6 and mono-L-aspartyl chlorin e6 (NPe6), against hepatitis C virus

  Hotta Hak, Aoki Chie, Suratno Lulut Ratnoglik, Pratiwi Sudarmono, Mari Komoto, Deng Lin, Shoji Ikuo, Hiroyuki Fuchino, Nobuo Kawahara

  20th International Symposium on Hepatitis C Virus and Related Viruses., Oct. 2013, English, Organising Committee of the20th International Symposium on Hepatitis C Virus and Related Viruses, Melbourne, Australia, International conference

  Poster presentation


• 抗HCV活性を有する薬用植物の探索

  渕野 裕之, 大根谷 章浩, 宮永 賢, Aoki Chie, Suratno Lulut Ratnoglik, Hotta Hak, 川原 信夫

  日本生薬学会第60回年会, Sep. 2013, Japanese, 日本生薬学会, 札幌, Domestic conference

  Poster presentation


• Antiviral Activity of Indonesian Plants from East Java Region Againts Hepatitis C Virus.

  Wahyuni Tutik Sri, Tumewu Lydia, Permatasari Adita Ayu, Apriani Evhy, Adianti Myrna, Rahman Abdul, Widyawaruyanti Aty, Lusida Maria Inge, Fuad Achamd, Soetjipto, Aoki Chie, Fuchino Hiroyuki, Kawahara Nobuo, Hotta Hak

  International Conference on Natural Products, Mar. 2013, English, Organising Committee of the International Conference on Natural ProductsViruses, Shah Alam, Malaysia., Hepatitis C virus (HCV) is a major cause of liver disease worldwide and a potential cause of substantial morbidity and mortality in the future. The most recent WHO estimate of the prevalence of HCV infection is 2%, representing 120 million people. Current standard of care is effective in only 50% of the patients, poorly tolerated, and associated with significant side effects an, International conference

  Oral presentation


• C型肝炎ウイルスに対する予防および治療ワクチン開発に関する研究

  Jiang Dapeng, Ratnoglik Lulut Suratno, Aoki Chie, Lin Deng, Shoji Ikuo, Hotta Hak

  第60回日本ウイルス学会学術集会, Nov. 2012, Japanese, 日本ウイルス学会, 大阪, 【目的と意義】Ｃ型肝炎ウイルス（HCV）慢性感染者は、日本で約200万人、全世界で約1億7,000万～2億人と推定されている。最近認可されたより治療効果の高い三者併用療法でも、30%近い症例で完全治癒が望めず、HCV 治療ワクチンと新たな感染者発生を防止する予防ワクチンの開発が強く求められている。我々は、HCVに対する予防ワクチンと治療ワクチンを開発する目的で、HCVのエンベロープタンパク質及び非構造タンパク質の一部をコードする遺伝子領域を組み込んだ発現プラスミドを数種類作製し、DNAワクチンとしてマウスに接種して、中和抗体産生及び細胞性免疫誘導を検討した。【材料と方法】1）DNAワクチン：HCV(1b型)のエンベロープタンパク質(E2)及び非構造タンパク質の一部(NS3)をコードする遺伝子領域を組み込んだ発現プラスミドを数種類作製した。2）マウス免, Domestic conference

  Oral presentation


• Development of therapeutic and preventive vaccines against Hepatitis C virus.

  Jiang Depeng, Ratnoglik Suratno Lulut, Aoki Chie, Deng Lin, Shoji Ikuo, Hotta Hak

  19th International Symposium on Hepatitis C Virus and Related Viruses., Oct. 2012, English, Organising Committee of the19th International Symposium on Hepatitis C Virus and Related Viruses, Venice, Italy, An estimated 170 to 200 million individuals are chronically infected with Hepatitis C virus (HCV) worldwide and 3 to 4 million individuals are newly infected each year. There is currently no vaccine available to protect against HCV. Considering the limited efficacy and high cost of treatment for chronic Hepatitis C, preventive and therapeutic vaccines against HCV are thus much, International conference

  Poster presentation


• Differential measurement of the number of plus- and minus- RNA molecules of hepatitis C virus in infected cells.

  Yagyu F, Aoki C, Yu L, Iwamoto A, Kitamura Y

  17th International Symposium on Hepatitis C Virus and Related Viruses, Oct. 2010, English, International conference


• Variants of hepatitis C virus JFH-1 strain adapted to huh7 cells.

  Lijuan Yu, Aoki Chie, Yohko Shimizu, Shimizu Kazufumi, Fumihiro Yagyu, Wei Hou, Masamichi Oshima, Aikichi Iwamoto, Bin Gao, Frank Wenjun Liu, George Fu Gao, Yoshihiro Kitamura

  17th International symposium on hepatitis C virus and related viruses, Sep. 2010, English, Organising Committee of the17th International Meeting on Hepatitis C Virus and Related Viruses, Yokohama, Japan, International conference

  Poster presentation


• Differential measurement of the number of plus- and minus- RNA molecules of hepatitis C virus in infected cells

  Fumihiro Yagyu, Aoki Chie, Lijuan Yu, Aikichi Iwamoto, Yoshihiro Kitamura

  17th International symposium on hepatitis C virus and related viruses, Sep. 2010, English, Organising Committee of the17th International Meeting on Hepatitis C Virus and Related Viruses, Yokohama, Japan, International conference

  Poster presentation


• Hepatitis C Virus Replicon with Gaussia Luciferase.

  Aoki C, Yagyu F, Shimizu Y, Shimizu K, Oshima M, Iwamoto A, Kitamura Y

  16th International Symposium on Hepatitis C Virus and Related Viruses, Oct. 2009, English, International conference


• Interferon-Induced Protein 44 (IFI44)に関する研究

  青木千恵, 清水洋子, 清水一史, 大島正道, 岩本愛吉, 北村義浩

  Oct. 2009, Japanese, Domestic conference


• Anti-HCV activity of Interferon-inducible proteins, IFI44/IFI44L.

  Aoki C, Yu L, Shimizu Y, Shimizu K, Hijikata M, Hou W, Yagyu F, Oshima M, Han Bing, Wei X, Xue Y, Iwamoto A, Gao B, Liu W, Gao GF, Kitamura Y

  The First China-Japan Joint Workshop on New-Generation vaccines for Infectious Diseases: Molecular Biology of Host-Pathogen Interaction, Jun. 2008, English, International conference

  Oral presentation


• Hepatitis C virus replication in cultured mammalian cells.

  Aoki C

  International Symposium on Basic and Applied Immunology, 2007, International conference


• デングウイルスエンベロープタンパク質と宿主由来糖鎖分子との相互作用の解析および糖鎖誘導体によるデングウイルス感染阻害効果の検討

  青木千恵, 左一八, 森田公一, 長谷部太, 高橋忠信, 鈴木隆, 鈴木康夫

  第53回日本ウイルス学会学術集会, Oct. 2005, Japanese, Domestic conference

  Oral presentation


• デング熱ウイルス結合性糖鎖分子の構造及びその性状解析

  青木千恵, 左一八, 森田公一, 長谷部太, 宮本大誠, 鈴木隆, 鈴木康夫

  第52回日本ウイルス学会学術集会, Oct. 2004, Japanese, Domestic conference


• Isolation and characterization Isolation and characterization of carbohydrate molecules recognized with dengue virus from human and mosquito cells.

  Aoki C, Hidari KI, Iwai K, Suzuki T, Miyamoto D, Morita K, Suzuki Y

  Pharmaceutical Sciences World Congress, 2004, International conference


• デング熱ウイルス結合性糖鎖分子の構造およびその性状解析

  青木千恵, 左一八, 森田公一, 長谷部太, 宮本大誠, 鈴木隆, 鈴木康夫

  第123回日本薬学会, Mar. 2003, Japanese, Domestic conference

  Poster presentation

• THE JAPANESE SOCIETY OF PHARMACOGNOSY


• THE PHARMACEUTICAL SOCIETY OF JAPAN


• THE JAPANESE SOCIETY FOR VIROLOGY

• 新型コロナウイルス感染増殖における脂質代謝系の役割の解明

  靭千恵

  木下基礎科学研究基金助成事業, Aug. 2021 - Mar. 2023, Principal investigator


• インドネシアの薬用植物からの抗 B 型肝炎ウイルス新規リード化合物の探索と開発研究

  靭 千恵

  日本学術振興会, 二国間交流事業, Apr. 2018 - Mar. 2021, Principal investigator

  Competitive research funding


• インドネシア産植物に含まれるフェノール性水酸基化合物からの新規肝炎ウイルス治療薬シーズの探索

  靭 千恵

  稲盛財団助成研究, Apr. 2018 - Sep. 2019, Principal investigator

  Competitive research funding


• インドネシアにおける肝疾患の早期診断治療への教育システムの確立

  林 祥剛

  科学研究費補助金／基盤研究(B), Apr. 2016 - Mar. 2019

  Competitive research funding