Research activity information

• Oct. 2025 神戸大学, 令和7年度 学長表彰

  蓮沼 誠久


• Sep. 2025 The Society Biotechnology, Japan, Biotechnology Paper Award, Beneficial effect of optimizing the expression balance of the mevalonate pathway introduced into the mitochondria on terpenoid production in Saccharomyces cerevisiae

  So Yanagibashi, Takahiro Bamba, Takayoshi Kirisako, Akihiko Kondo, Tomohisa Hasunuma


• Oct. 2024 神戸大学, 令和6年度 学長表彰

  蓮沼 誠久


• Oct. 2023 神戸大学, 令和5年度 学長表彰

  蓮沼 誠久


• Oct. 2022 神戸大学, 令和4年度 学長表彰

  蓮沼誠久


• Oct. 2021 神戸大学, 令和3年度 学長表彰

  蓮沼誠久


• Oct. 2020 神戸大学, 令和2年度 学長表彰

  蓮沼誠久


• Oct. 2019 神戸大学, 第11回学長表彰

  蓮沼 誠久


• Oct. 2018 神戸大学, 第10回学長表彰

  蓮沼 誠久


• Oct. 2017 神戸大学, 第9回学長表彰

  蓮沼 誠久


• Sep. 2014 The Society for Biotechnology, Japan, Biotechnology Encouragement Prize, 代謝プロファイリングに基づく微生物育種技術の開発と応用

  HASUNUMA Tomohisa


• Oct. 2013 Japan Bioindustry Association, Fermentation and Metabolism Research Prize, システムバイオロジー解析に基づく高機能型酵母創製とセルロースエタノール高生産技術開発への応用

  HASUNUMA Tomohisa

• Astaxanthin Overproduction Enhanced by Metabolomics-Guided Rational Metabolic Engineering in Synechococcus sp. PCC 7002.

  Kousuke Ida, Kenya Tanaka, Yuichi Kato, Nobuaki Koike, Yoji Horie, Mami Matsuda, Hisashi Yasueda, Akihiko Kondo, Tomohisa Hasunuma

  Astaxanthin, a natural red pigment with antioxidant and other physiological activities, is widely used in the feed, pharmaceutical, and cosmetic industries. Haematococcus pluvialis is a well-known microbial producer of astaxanthin; however, its slow growth and requirement for a complex two-stage cultivation under high-light conditions limit large-scale application due to increased contamination risk. As an alternative, Synechococcus sp. PCC 7002 offers rapid growth and robustness, but metabolic engineering strategies to enhance astaxanthin production in this strain remain underexplored. In this study, we applied a metabolomics-guided approach to identify novel metabolic bottlenecks and engineering targets. A base strain expressing β-carotene hydroxylase (crtZ) and ketolase (crtW) from Brevundimonas sp. SD212 produced 6.2 mg/g of DCW astaxanthin. Metabolome analysis revealed the accumulation of sedoheptulose-7-phosphate (S7P) and 2-C-methyl-d-erythritol-2,4-cyclopyrophosphate (MEcPP), suggesting that the reactions catalyzed by transketolase (TKT) and (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate synthase (IspG) are rate-limiting. Overexpression of tkt or ispG reduced the levels of their respective substrates, confirming relief of these bottlenecks. Notably, the TKT-overexpressing strain achieved an astaxanthin content of 10.3 mg/g-DCW, while the IspG-overexpressing strain showed no significant improvement. Further optimization of culture conditions─such as medium composition, light intensity, and temperature─led to an astaxanthin productivity of 7.5 mg/L/day. These results demonstrate the effectiveness of a metabolomics-driven design-build-test-learn (DBTL) approach for enhancing astaxanthin production in Synechococcus sp. PCC 7002.

  Nov. 2025, ACS synthetic biology, English, International magazine

  Scientific journal


• Identification of Subfamily Specific Residues within Highly Active and Promiscuous Alcohol Dehydrogenases

  Ryota Hidese, Kanae Sakai, Musashi Takenaka, Keiji Fushimi, Hisashi Kudo, Kenya Tanaka, Ryo Nasuno, Christopher J. Vavricka, Akihiko Kondo, Tomohisa Hasunuma

  American Chemical Society (ACS), Jun. 2025, ACS Catalysis, 15(14) (14), 11931 - 11943

  Scientific journal


• Metabolic engineering and cultivation strategies for efficient production of fucoxanthin and related carotenoids.

  Kenya Tanaka, John Chi-Wei Lan, Akihiko Kondo, Tomohisa Hasunuma

  Fucoxanthin, a bioactive carotenoid derived from algae, has attracted considerable attention for its applications in health, cosmetics, and nutrition. Advances in metabolic engineering, such as the overexpression of pathway-specific enzymes and enhancement of precursor availability, have shown promising results in improving production efficiency. However, despite its high value, the biosynthetic pathway of fucoxanthin remains only partially elucidated, posing significant challenges for metabolic engineering efforts. Recent studies have identified previously unknown enzymes and regulatory elements within the pathway, providing opportunities for further productivity enhancements through targeted metabolic modifications. Additionally, adaptive evolution, mutagenesis-driven strain development, and optimized cultivation conditions have demonstrated significant potential to boost fucoxanthin yields. This review consolidates the latest insights into the biosynthetic pathway of fucoxanthin and highlights metabolic engineering strategies aimed at enhancing the production of fucoxanthin and related carotenoids, offering approaches to design high-yielding strains. Furthermore, recent advancements in random mutagenesis and cultivation technology are discussed. By integrating these developments, more economically viable and environmentally sustainable fucoxanthin production systems can be achieved. KEY POINTS : • Insights into fucoxanthin biosynthesis enable targeted metabolic engineering. • ALE and cultivation strategies complement metabolic engineering efforts. • Balanced push-pull-block strategies improve fucoxanthin production efficiency.

  Mar. 2025, Applied microbiology and biotechnology, 109(1) (1), 57 - 57, English, International magazine

  Scientific journal


• Development of the autonomous lab system to support biotechnology research.

  Keiji Fushimi, Yusuke Nakai, Akiko Nishi, Ryo Suzuki, Masahiro Ikegami, Risa Nimura, Taichi Tomono, Ryota Hidese, Hisashi Yasueda, Yusuke Tagawa, Tomohisa Hasunuma

  In this study, we developed the autonomous lab (ANL), which is a system based on robotics and artificial intelligence (AI) to conduct biotechnology experiments and formulate scientific hypotheses. This system was designed with modular devices and Bayesian optimization algorithms, allowing it to effectively run a closed loop from culturing to preprocessing, measurement, analysis, and hypothesis formulation. As a case study, we used the ANL to optimize medium conditions for a recombinant Escherichia coli strain, which overproduces glutamic acid. The results demonstrated that our autonomous system successfully replicated the experimental techniques, such as sample preparation and data measurement, and improved both the cell growth rate and the maximum cell growth. The ANL offers a versatile and scalable solution for various applications in the field of bioproduction, with the potential to improve efficiency and reliability of experimental processes in the future.

  Feb. 2025, Scientific reports, 15(1) (1), 6648 - 6648, English, International magazine

  Scientific journal


• Introduction of acetyl-phosphate bypass and increased culture temperatures enhanced growth-coupled poly-hydroxybutyrate production in the marine cyanobacterium Synechococcus sp. PCC7002.

  Kosuke Inabe, Ryota Hidese, Yuichi Kato, Mami Matsuda, Takanobu Yoshida, Keiji Matsumoto, Akihiko Kondo, Shunsuke Sato, Tomohisa Hasunuma

  Polyhydroxyalkanoate (PHA) is an attractive bio-degradable plastic alternative to petrochemical plastics. Photosynthetic cyanobacteria accumulate biomass by fixing atmospheric CO2, making them promising hosts for sustainable PHA production. Conventional PHA production in cyanobacteria requires prolonged cultivation under nutrient limitation to accumulate cellular PHA. In this study, we developed a system for growth-coupled production of the PHA poly-hydroxybutyrate (PHB), using the marine cyanobacterium Synechococcus sp. PCC 7002. A recombinant strain termed KB1 expressing a set of heterologous PHB biosynthesis genes (phaA/phaB from Cupriavidus necator H16 and phaE/phaC from Synechocystis sp. PCC 6803) accumulated substantial PHB during growth (11.4% of dry cell weight). To improve PHB accumulation, we introduced the Pseudomonas aeruginosa phosphoketolase gene (pk) into strain KB1, rewiring intermediates of the Calvin-Benson-Bassham (CBB) cycle (xyluose-5-phosphate, sedoheptulose 7-phosphate, and fructose-6-phosphate) to acetyl-CoA. The pk-expressing strain, KB15, accumulated 2.1-fold enhanced levels of PHB (23.8% of dried cell weight), relative to the parent strain, KB1. The highest PHB titer of KB15 strain supplemented with acetate was about 1.1 g L-1 and the yield was further enhanced by 2.6-fold following growth at 38 °C (0.21 g L-1 d-1), relative to growth at 30 °C. Metabolome analysis revealed that pool sizes of CBB intermediates decreased, while levels of acetyl-CoA increased in strain KB15 compared with strain KB1, and this increase was further enhanced following growth at 38 °C. Our data demonstrate that acetyl-phosphate generated by Pk was converted into acetyl-CoA via acetate by hitherto unidentified enzymes. In conclusion, expression of heterologous PHB biosynthesis genes enabled growth-coupled PHB production in strain PCC 7002, which was increased through acetyl-CoA supplementation by bypassing acetyl-phosphate and elevating culture temperature.

  Jan. 2025, Metabolic engineering, 88, 228 - 239, English, International magazine

  Scientific journal


• Activation of the pentose phosphate pathway by microcurrent stimulation mediates antioxidant effects in inflammation-stimulated macrophages.

  Mikiko Uemura, Noriaki Maeshige, Atomu Yamaguchi, Xiaoqi Ma, Yunfei Fu, Taketo Inoue, Mami Matsuda, Yuya Nishimura, Tomohisa Hasunuma, Ji Wang, Hiroyo Kondo, Hidemi Fujino

  INTRODUCTION: Excessive inflammatory responses in macrophages lead to increased oxidative stress, and the excessive production of reactive oxygen species (ROS) causes tissue damage, contributing to the development of chronic diseases and tissue deterioration. Therefore, controlling the inflammatory response and ROS production is crucial for human health. Electrical stimulation (ES) has been shown to have antioxidant and anti-inflammatory effects on macrophages. However, the key pathway underlying these effects remains unclear. METHODS: In this study, ES was applied to Lipopolysaccharide (LPS)-stimulated macrophages, and the production of ROS and 8-hydroxy-2'-deoxyguanosine (8-OHdG), inflammatory cytokine expression, and intracellular metabolites were analyzed in a glucose-6-phosphate dehydrogenase (G6PD) knockdown experiment, the rate-limiting enzyme of the Pentose Phosphate Pathway(PPP). RESULTS: ES significantly increased sedoheptulose 7-phosphate (S7P), an intermediate metabolite in PPP, and reduced ROS and 8-OHdG production and the expression of inflammatory cytokines in LPS-stimulated macrophages. Meanwhile, ES did not exert antioxidant effects in G6PD-knockdown macrophages. DISCUSSION: These findings indicate that the antioxidant effects of ES are mediated by PPP in LPS-stimulated macrophages.

  2025, Frontiers in physiology, 16, 1666999 - 1666999, English, International magazine

  Scientific journal


• Metabolic analysis reveals the contribution of mechanosensitive channel MscM to extracellular release of glutamate in glycogen-deficient Synechococcus elongatus.

  Yuichi Kato, Kouhei Kamasaka, Mami Matsuda, Hiroko Koizumi, Ryudo Ohbayashi, Hiroki Ashida, Akihiko Kondo, Tomohisa Hasunuma

  In bacteria, mechanosensitive channels mediate extracellular release of osmolytes, including glutamate, functioning as safety valves upon osmotic downshift. In cyanobacteria, the role of mechanosensitive channels has not been completely elucidated. Recently, the glycogen-deficient ΔglgC mutant of Synechococcus elongatus PCC 7942 was found to release glutamate extracellularly, giving rise to a hypothesis that the role of mechanosensitive channels in cyanobacteria is conserved. Using the ΔglgC mutant as the model, the present study aimed to examine whether the putative mechanosensitive channel protein MscM mediates the extracellular release of glutamate. Compared to the ΔglgC mutant, the ΔglgC ΔmscM mutant was found to release less glutamate and aspartate extracellularly. In addition, intracellular levels of these amino acids were significantly higher in the ΔglgC ΔmscM mutant than in the ΔglgC mutant. These results suggested that MscM mediates the extracellular release of glutamate and aspartate in glycogen-deficient cyanobacteria. Furthermore, the ΔglgC ΔmscM mutant exhibited more elongated cell shapes compared to the wild type and ΔglgC single mutant, suggesting that the deletion of the mscM gene intensified turgor pressure and/or that MscM is involved in cell division. Through metabolic analysis, the present study revealed that mechanosensitive channel MscM in cyanobacteria is involved in the extracellular release of amino acids.

  Dec. 2024, Journal of bioscience and bioengineering, English, Domestic magazine

  Scientific journal


• Genome-scale prediction of gene ontology from mass fingerprints reveals new metabolic gene functions

  Christopher J. Vavricka, Masao Mochizuki, Satoshi Yuzawa, Masahiro Murata, Takanobu Yoshida, Naoki Watanabe, Masahiko Nakatsui, Jun Ishii, Kiyotaka Hara, Hal S. Alper, Tomohisa Hasunuma, Akihiko Kondo, Michihiro Araki

  Abstract Mass-based fingerprinting can characterize unknown strains, however expansion of these methods to predict specific gene functions is lacking. Therefore, rapid mass fingerprinting was developed to functionally profile a comprehensive yeast knockout library. Matrix assisted laser desorption ionization (MALDI)-time of flight (TOF) mass fingerprints of 3,238 Saccharomyces cerevisiae knockouts were digitized for correlation with gene ontology (GO) annotations. Random forests and support vector machine (SVM) algorithms precisely assigned GO accessions with AUC scores all above 0.83. SVM was the best predictor with average true positive and true negative rates of 0.975 and 0.991, respectively. The SVM model suggested new functions for 28 uncharacterized yeast genes. Metabolomics analysis of two knockouts (YDR215C and YLR122C) of uncharacterized genes predicted to be involved in methylation-related metabolism, showed altered intracellular contents of methionine-related metabolites. Increased S-adenosylmethionine in YDR215C highlights potential for enhancement of methylation pathways. These results demonstrate that MALDI-TOF fingerprints can be rapidly digitized, resulting in datasets that enable prediction of microbial genotypes and even the function of specific genes. This fingerprinting method can inform optimal bioproduction chassis selection.

  Cold Spring Harbor Laboratory, Dec. 2024


• De Novo Production of the Bioactive Phenylpropanoid Artepillin C Using Membrane-Bound Prenyltransferase in Komagataella phaffii

  Takahiro Bamba, Ryosuke Munakata, Yuya Ushiro, Ryota Kumokita, Sayaka Tanaka, Yoshimi Hori, Akihiko Kondo, Kazufumi Yazaki, Tomohisa Hasunuma

  American Chemical Society (ACS), Nov. 2024, ACS Synthetic Biology, 13(12) (12), 4040 - 4049

  Scientific journal


• Pioneering precision in markerless strain development for Synechococcus sp. PCC 7002.

  Ayaka Tsuji, Kosuke Inabe, Ryota Hidese, Yuichi Kato, Lucília Domingues, Akihiko Kondo, Tomohisa Hasunuma

  Marine cyanobacteria such as Picosynechococcus sp. (formerly called Synechococcus sp.) PCC 7002 are promising chassis for photosynthetic production of commodity chemicals with low environmental burdens. Genetic engineering of cyanobacteria conventionally employs antibiotic resistance markers. However, limited availability of antibiotic-resistant markers is a problem for highly multigenic strain engineering. Although several markerless genetic manipulation methods have been developed for PCC 7002, they often lack versatility due to the requirement of gene disruption in the host strain. To achieve markerless transformation in Synechococcus sp. with no requirements for the host strain, this study developed a method in which temporarily introduces a mutated phenylalanyl-tRNA synthetase gene (pheS) into the genome for counter selection. Amino acid substitutions in the PheS that cause high susceptibility of PCC 7002 to the phenylalanine analog p-chlorophenylalanine were examined, and the combination of T261A and A303G was determined as the most suitable mutation. The mutated PheS-based selection was utilized for the markerless knockout of the nblA gene in PCC 7002. In addition, the genetic construct containing the lldD and lldP genes from Escherichia coli was introduced into the ldhA gene site using the counter selection strategy, resulting in a markerless recombinant strain. The repeatability of this method was demonstrated by the double markerless knockin recombinant strain, suggesting it will be a powerful tool for multigenic strain engineering of cyanobacteria.

  Oct. 2024, Microbial cell factories, 23(1) (1), 268 - 268, English, International magazine

  Scientific journal


• Recombinant lactate-assimilating cyanobacteria reduce high-concentration culture-associated cytotoxicity in mammalian cells.

  Yuji Haraguchi, Yuichi Kato, Ayaka Tsuji, Tomohisa Hasunuma, Tatsuya Shimizu

  In the fields of cultured meat, biopharmaceuticals, cell therapy, and tissue engineering, large numbers of mammalian cells are required; thus, highly-concentrated cell cultures are widely adopted. In general, such cultures can lead to cell damage caused by waste product accumulation and nutritional inadequacy. In this study, a novel co-culture system where the recombinant lactate-assimilating cyanobacterial strain, KC0110, derived from euryhaline Picosynechococcus sp. PCC 7002, and mammalian muscle cells cultured across porous membranes been developed. By using the KC0110 strain, the amount of ammonium and lactate excreted from C2C12 mouse muscle cells into the culture significantly decreased. Importantly, pyruvate and some amino acids, including pyruvate-derived amino acids, also increased significantly compared to those in monoculture of C2C12 cells. It is believed that the organic acids secreted by the KC0110 strain enhance the growth of mammalian cells, leading to a reduction in high-concentration culture-induced mammalian cell damage [lactate dehydrogenase (LDH) release] through cyanobacterial co-culture. These results show that, through co-cultivation with cyanobacteria, it is possible to culture mammalian cells, alleviating cell damage, even in highly-concentrated cultures. This study demonstrated an in vitro "symbiotic circular system" that can interchange metabolites produced by phototrophs and mammalian cells.

  Oct. 2024, Archives of microbiology, 206(11) (11), 425 - 425, English, International magazine

  Scientific journal


• Minimized dark consumption of Calvin cycle intermediates facilitates the initiation of photosynthesis in Synechocystis sp. PCC 6803.

  Kenya Tanaka, Akihiko Kondo, Tomohisa Hasunuma

  Cyanobacteria intricately regulate their metabolic pathways during the diurnal cycle to ensure survival and growth. Under dark conditions, the breakdown of glycogen, an energy reserve, in these organisms replenishes Calvin cycle intermediates, especially downstream glycolytic metabolites, which are necessary for photosynthesis initiation upon light irradiation. However, it remains unclear how the accumulation of these intermediates is maintained in the dark despite limited glycogen availability. Therefore, in this study, we investigated the regulation of downstream glycolytic metabolites of the Calvin cycle under dark and light treatment using Synechocystis sp. PCC 6803. Our results showed that during the dark period, low pyruvate kinase (Pyk) activity ensured metabolite accumulation, while endogenous Pyk overexpression significantly lowered the accumulation of glycolytic intermediates. Remarkably, wild type Synechocystis maintained oxygen evolution ability throughout dark treatment for over 2 d, while Pyk overexpression resulted in decreased oxygen evolution after 16 h of dark treatment. These results indicated that limiting Pyk activity via darkness treatment facilitates photosynthetic initiation by maintaining glycolytic intermediates. Similarly, phosphoenolpyruvate carboxylase (PepC) overexpression decreased oxygen evolution under dark treatment; however, its effect was lower than that of Pyk. Further, we noted that as PepC overexpression decreased the levels of glycolytic intermediates in the dark, sugar phosphates in the Calvin-Benson-Bassham (CBB) cycle showed high accumulation, suggesting that sugar phosphates play important roles in supporting photosynthesis initiation. Therefore, our study highlights the importance of controlling the metabolic pathways through which glycolytic and CBB cycle intermediates are consumed (defined as cataplerosis of CBB cycle) to ensure stable photosynthesis.

  Sep. 2024, Plant & cell physiology, English, Domestic magazine

  Scientific journal


• A serum-free culture medium production system by co-culture combining growth factor-secreting cells and L-lactate-assimilating cyanobacteria for sustainable cultured meat production.

  Shanga Chu, Yuji Haraguchi, Toru Asahi, Yuichi Kato, Akihiko Kondo, Tomohisa Hasunuma, Tatsuya Shimizu

  Large-scale production of cultured meat requires bulk culture medium containing growth-promoting proteins from animal serum. However, animal serum for mammalian cell culture is associated with high costs, ethical concerns, and contamination risks. Owing to its growth factor content, conditioned medium from rat liver epithelial RL34 cells can replace animal serum for myoblast proliferation. More seeded cells and longer culture periods are thought to yield higher growth factor levels, resulting in more effective muscle cell proliferation. However, RL34 cells can deplete nutrients and release harmful metabolites into the culture medium over time, potentially causing growth inhibition and apoptosis. This issue highlights the need for waste clearance during condition medium production. To address this issue, we introduced a lactate permease gene (lldP) and an L-lactate-to-pyruvate conversion enzyme gene (lldD) to generate a recombinant L-lactate-assimilating cyanobacterium Synechococcus sp. KC0110 strain. Transwell co-culture of this strain with RL34 cells exhibited a marked reduction in the levels of harmful metabolites, lactate and ammonium, while maintaining higher concentrations of glucose, pyruvate, and pyruvate-derived amino acids than those seen with RL34 cell monocultures. The co-culture medium supported myoblast proliferation without medium dilution or additional nutrients, which was attributed to the waste clearance and nutrient replenishment effects of the KC0110 strain. This culture system holds potential for the production of low-cost, and animal-free cultured meat.

  Aug. 2024, Scientific reports, 14(1) (1), 19578 - 19578, English, International magazine

  Scientific journal


• Engineering Escherichia coli for efficient glutathione production

  Hiroki Mori, Misato Matsui, Takahiro Bamba, Yoshimi Hori, Sayaka Kitamura, Yoshihiro Toya, Ryota Hidese, Hisashi Yasueda, Tomohisa Hasunuma, Hiroshi Shimizu, Naoaki Taoka, Shingo Kobayashi

  Elsevier BV, Jul. 2024, Metabolic Engineering, 84, 180 - 190

  Scientific journal


• Integrated pathway mining and selection of an artificial CYP79-mediated bypass to improve benzylisoquinoline alkaloid biosynthesis.

  Musashi Takenaka, Kouhei Kamasaka, Kim Daryong, Keiko Tsuchikane, Seiha Miyazawa, Saeko Fujihana, Yoshimi Hori, Christopher J Vavricka, Akira Hosoyama, Hiroko Kawasaki, Tomokazu Shirai, Michihiro Araki, Akira Nakagawa, Hiromichi Minami, Akihiko Kondo, Tomohisa Hasunuma

  BACKGROUND: Computational mining of useful enzymes and biosynthesis pathways is a powerful strategy for metabolic engineering. Through systematic exploration of all conceivable combinations of enzyme reactions, including both known compounds and those inferred from the chemical structures of established reactions, we can uncover previously undiscovered enzymatic processes. The application of the novel alternative pathways enables us to improve microbial bioproduction by bypassing or reinforcing metabolic bottlenecks. Benzylisoquinoline alkaloids (BIAs) are a diverse group of plant-derived compounds with important pharmaceutical properties. BIA biosynthesis has developed into a prime example of metabolic engineering and microbial bioproduction. The early bottleneck of BIA production in Escherichia coli consists of 3,4-dihydroxyphenylacetaldehyde (DHPAA) production and conversion to tetrahydropapaveroline (THP). Previous studies have selected monoamine oxidase (MAO) and DHPAA synthase (DHPAAS) to produce DHPAA from dopamine and oxygen; however, both of these enzymes produce toxic hydrogen peroxide as a byproduct. RESULTS: In the current study, in silico pathway design is applied to relieve the bottleneck of DHPAA production in the synthetic BIA pathway. Specifically, the cytochrome P450 enzyme, tyrosine N-monooxygenase (CYP79), is identified to bypass the established MAO- and DHPAAS-mediated pathways in an alternative arylacetaldoxime route to DHPAA with a peroxide-independent mechanism. The application of this pathway is proposed to result in less formation of toxic byproducts, leading to improved production of reticuline (up to 60 mg/L at the flask scale) when compared with that from the conventional MAO pathway. CONCLUSIONS: This study showed improved reticuline production using the bypass pathway predicted by the M-path computational platform. Reticuline production in E. coli exceeded that of the conventional MAO-mediated pathway. The study provides a clear example of the integration of pathway mining and enzyme design in creating artificial metabolic pathways and suggests further potential applications of this strategy in metabolic engineering.

  Jun. 2024, Microbial cell factories, 23(1) (1), 178 - 178, English, International magazine

  Scientific journal


• Mixotrophic culture enhances fucoxanthin production in the haptophyte Pavlova gyrans.

  Erina Yoshida, Yuichi Kato, Akihiko Kanamoto, Akihiko Kondo, Tomohisa Hasunuma

  Fucoxanthin is a versatile substance in the food and pharmaceutical industries owing to its excellent antioxidant and anti-obesity properties. Several microalgae, including the haptophyte Pavlova spp., can produce fucoxanthin and are potential industrial fucoxanthin producers, as they lack rigid cell walls, which facilitates fucoxanthin extraction. However, the commercial application of Pavlova spp. is limited owing to insufficient biomass production. In this study, we aimed to develop a mixotrophic cultivation method to increase biomass and fucoxanthin production in Pavlova gyrans OPMS 30543X. The effects of culturing OPMS 30543X with different organic carbon sources, glycerol concentrations, mixed-nutrient conditions, and light intensities on the consumption of organic carbon sources, biomass production, and fucoxanthin accumulation were analyzed. Several organic carbon sources, such as glycerol, glucose, sucrose, and acetate, were examined, revealing that glycerol was well-consumed by the microalgae. Biomass and fucoxanthin production by OPMS 30543X increased in the presence of 10 mM glycerol compared to that observed without glycerol. Metabolomic analysis revealed higher levels of the metabolites related to the glycolytic, Calvin-Benson-Bassham, and tricarboxylic acid cycles under mixotrophic conditions than under autotrophic conditions. Cultures grown under mixotrophic conditions with a light intensity of 100 µmol photons m-2 s-1 produced more fucoxanthin than autotrophic cultures. Notably, the amount of fucoxanthin produced (18.9 mg/L) was the highest reported thus far for Pavlova species. In conclusion, the use of mixotrophic culture is a promising strategy for increasing fucoxanthin production in Pavlova species. KEY POINTS: • Glycerol enhances biomass and fucoxanthin production in Pavlova gyrans • Metabolite levels increase under mixotrophic conditions • Mixotrophic conditions and medium-light intensity are appropriate for P. gyrans.

  May 2024, Applied microbiology and biotechnology, 108(1) (1), 352 - 352, English, International magazine

  Scientific journal


• Glycogen deficiency enhances carbon partitioning into glutamate for an alternative extracellular metabolic sink in cyanobacteria.

  Yuichi Kato, Ryota Hidese, Mami Matsuda, Ryudo Ohbayashi, Hiroki Ashida, Akihiko Kondo, Tomohisa Hasunuma

  Glycogen serves as a metabolic sink in cyanobacteria. Glycogen deficiency causes the extracellular release of distinctive metabolites such as pyruvate and 2-oxoglutarate upon nitrogen depletion; however, the mechanism has not been fully elucidated. This study aimed to elucidate the mechanism of carbon partitioning in glycogen-deficient cyanobacteria. Extracellular and intracellular metabolites in a glycogen-deficient ΔglgC mutant of Synechococcus elongatus PCC 7942 were comprehensively analyzed. In the presence of a nitrogen source, the ΔglgC mutant released extracellular glutamate rather than pyruvate and 2-oxoglutarate, whereas its intracellular glutamate level was lower than that in the wild-type strain. The de novo synthesis of glutamate increased in the ΔglgC mutant, suggesting that glycogen deficiency enhanced carbon partitioning into glutamate and extracellular excretion through an unidentified transport system. This study proposes a model in which glutamate serves as the prime extracellular metabolic sink alternative to glycogen when nitrogen is available.

  Feb. 2024, Communications biology, 7(1) (1), 233 - 233, English, International magazine

  Scientific journal


• High-level phenol bioproduction by engineered Pichia pastoris in glycerol fed-batch fermentation using an efficient pertraction system

  Ryota Kumokita, Takahiro Bamba, Hisashi Yasueda, Ayato Tsukida, Keizo Nakagawa, Tooru Kitagawa, Tomohisa Yoshioka, Hideto Matsuyama, Yasuhito Yamamoto, Satoshi Maruyama, Takahiro Hayashi, Akihiko Kondo, Tomohisa Hasunuma

  Elsevier BV, Feb. 2024, Bioresource Technology, 393, 130144 - 130144

  Scientific journal


• High-throughput evaluation of hemolytic activity through precise measurement of colony and hemolytic zone sizes of engineered Bacillus subtilis on blood agar

  Takahiro Bamba, Rina Aoki, Yoshimi Hori, Shu Ishikawa, Ken-ichi Yoshida, Naoaki Taoka, Shingo Kobayashi, Hisashi Yasueda, Akihiko Kondo, Tomohisa Hasunuma

  Abstract Biosurfactants have remarkable characteristics, such as environmental friendliness, high safety, and excellent biodegradability. Surfactin is one of the best-known biosurfactants produced by Bacillus subtilis. Because the biosynthetic pathways of biosurfactants, such as surfactin, are complex, mutagenesis is a useful alternative to typical metabolic engineering approaches for developing high-yield strains. Therefore, there is a need for high-throughput and accurate screening methods for high-yield strains derived from mutant libraries. The blood agar lysis method, which takes advantage of the hemolytic activity of biosurfactants, is one way of determining their concentration. This method includes inoculating microbial cells onto blood-containing agar plates, and biosurfactant production is assessed based on the size of the hemolytic zone formed around each colony. Challenges with the blood agar lysis method include low experimental reproducibility and a lack of established protocols for high-throughput screening. Therefore, in this study, we investigated the effects of the inoculation procedure and media composition on the formation of hemolytic zones. We also developed a workflow to evaluate the number of colonies using robotics. The results revealed that by arranging colonies at appropriate intervals and measuring the areas of colonies and hemolytic rings using image analysis software, it was possible to accurately compare the hemolytic activity among several colonies. Although the use of the blood agar lysis method for screening is limited to surfactants exhibiting hemolytic activity, it is believed that by considering the insights gained from this study, it can contribute to the accurate screening of strains with high productivity.

  Oxford University Press (OUP), Jan. 2024, Biology Methods and Protocols, 9(1) (1)

  Scientific journal


• Metabolomics-based development of bioproduction processes toward industrial-scale production.

  Kenya Tanaka, Takahiro Bamba, Akihiko Kondo, Tomohisa Hasunuma

  Microbial biomanufacturing offers a promising, environment-friendly platform for next-generation chemical production. However, its limited industrial implementation is attributed to the slow production rates of target compounds and the time-intensive engineering of high-yield strains. This review highlights how metabolomics expedites bioproduction development, as demonstrated through case studies of its integration into microbial strain engineering, culture optimization, and model construction. The Design-Build-Test-Learn (DBTL) cycle serves as a standard workflow for strain engineering. Process development, including the optimization of culture conditions and scale-up, is crucial for industrial production. In silico models facilitate the development of strains and processes. Metabolomics is a powerful driver of the DBTL framework, process development, and model construction.

  Dec. 2023, Current opinion in biotechnology, 85, 103057 - 103057, English, International magazine

  Scientific journal


• ppGpp accumulation reduces the expression of the global nitrogen homeostasis-modulating NtcA regulon by affecting 2-oxoglutarate levels

  Ryota Hidese, Ryudo Ohbayashi, Yuichi Kato, Mami Matsuda, Kan Tanaka, Sousuke Imamura, Hiroki Ashida, Akihiko Kondo, Tomohisa Hasunuma

  Abstract The cyanobacterium Synechococcus elongatus PCC 7942 accumulates alarmone guanosine tetraphosphate (ppGpp) under stress conditions, such as darkness. A previous study observed that artificial ppGpp accumulation under photosynthetic conditions led to the downregulation of genes involved in the nitrogen assimilation system, which is activated by the global nitrogen regulator NtcA, suggesting that ppGpp regulates NtcA activity. However, the details of this mechanism have not been elucidated. Here, we investigate the metabolic responses associated with ppGpp accumulation by heterologous expression of the ppGpp synthetase RelQ. The pool size of 2-oxoglutarate (2-OG), which activates NtcA, is significantly decreased upon ppGpp accumulation. De novo ¹³C-labeled CO₂ assimilation into the Calvin-Benson-Bassham cycle and glycolytic intermediates continues irrespective of ppGpp accumulation, whereas the labeling of 2-OG is significantly decreased under ppGpp accumulation. The low 2-OG levels in the RelQ overexpression cells could be because of the inhibition of metabolic enzymes, including aconitase, which are responsible for 2-OG biosynthesis. We propose a metabolic rearrangement by ppGpp accumulation, which negatively regulates 2-OG levels to maintain carbon and nitrogen balance.

  Springer Science and Business Media LLC, Dec. 2023, Communications Biology, 6(1) (1)

  Scientific journal


• The Carbon Flow Shifts from Primary to Secondary Metabolism during Xylem Vessel Cell Differentiation in Arabidopsis thaliana.

  Abigail Loren Tung Uy, Atsushi Yamamoto, Mami Matsuda, Toshihiro Arae, Tomohisa Hasunuma, Taku Demura, Misato Ohtani

  Xylem vessel cell differentiation is characterized by the deposition of a secondary cell wall (SCW) containing cellulose, hemicellulose and lignin. VASCULAR-RELATED NAC-DOMAIN7 (VND7), a plant-specific NAC (NAM, ATAF1/2, and CUC2) transcription factor, is a master regulator of xylem vessel cell differentiation in Arabidopsis (Arabidopsis thaliana). Previous metabolome analysis using the VND7-inducible system in tobacco BY-2 cells successfully revealed significant quantitative changes in primary metabolites during xylem vessel cell differentiation. However, the flow of primary metabolites is not yet well understood. Here, we performed a metabolomic analysis of VND7-inducible Arabidopsis T87 suspension cells. Capillary electrophoresis-time-of-flight mass spectrometry quantified 57 metabolites, and subsequent data analysis highlighted active changes in the levels of UDP-glucose and phenylalanine, which are building blocks of cellulose and lignin, respectively. In a metabolic flow analysis using stable carbon 13 (13C) isotope, the 13C-labeling ratio specifically increased in 3-phosphoglycerate after 12 h of VND7 induction, followed by an increase in shikimate after 24 h of induction, while the inflow of 13C into lactate from pyruvate was significantly inhibited, indicating an active shift of carbon flow from glycolysis to the shikimate pathway during xylem vessel cell differentiation. In support of this notion, most glycolytic genes involved in the downstream of glyceraldehyde 3-phosphate were downregulated following the induction of xylem vessel cell differentiation, whereas genes for the shikimate pathway and phenylalanine biosynthesis were upregulated. These findings provide evidence for the active shift of carbon flow from primary metabolic pathways to the SCW polymer biosynthetic pathway at specific points during xylem vessel cell differentiation.

  Dec. 2023, Plant & cell physiology, 64(12) (12), 1563 - 1575, English, Domestic magazine

  Scientific journal


• Electrical stimulation facilitates NADPH production in pentose phosphate pathway and exerts an anti-inflammatory effect in macrophages.

  Mikiko Uemura, Noriaki Maeshige, Atomu Yamaguchi, Xiaoqi Ma, Mami Matsuda, Yuya Nishimura, Tomohisa Hasunuma, Taketo Inoue, Jiawei Yan, Ji Wang, Hiroyo Kondo, Hidemi Fujino

  Macrophages play an important role as effector cells in innate immune system. Meanwhile, macrophages activated in a pro-inflammatory direction alter intracellular metabolism and damage intact tissues by increasing reactive oxygen species (ROS). Electrical stimulation (ES), a predominant physical agent to control metabolism in cells and tissues, has been reported to exert anti-inflammatory effect on immune cells. However, the mechanism underlying the anti-inflammatory effects by ES is unknown. This study aimed to investigate the effect of ES on metabolism in glycolytic-tricarboxylic acid cycle (TCA) cycle and inflammatory responses in macrophages. ES was performed on bone marrow-derived macrophages and followed by a stimulation with LPS. The inflammatory cytokine expression levels were analyzed by real-time polymerase chain reaction and ELISA. ROS production was analyzed by CellRox Green Reagent and metabolites by capillary electrophoresis-mass spectrometry. As a result, ES significantly reduced proinflammatory cytokine expression levels and ROS generation compared to the LPS group and increased glucose-1-phosphate, a metabolite of glycogen. ES also increased intermediate metabolites of the pentose phosphate pathway (PPP); ribulose-5-phosphate, rebose-5 phosphate, and nicotinamide adenine dinucleotide phosphate, a key factor of cellular antioxidation systems, as well as α-Ketoglutarate, an anti-oxidative metabolite in the TCA cycle. Our findings imply that ES enhanced NADPH production with enhancement of PPP, and also decreased oxidative stress and inflammatory responses in macrophages.

  Oct. 2023, Scientific reports, 13(1) (1), 17819 - 17819, English, International magazine

  Scientific journal


• Circular cell culture for sustainable food production using recombinant lactate-assimilating cyanobacteria that supplies pyruvate and amino acids

  Yuji Haraguchi, Yuichi Kato, Kosuke Inabe, Akihiko Kondo, Tomohisa Hasunuma, Tatsuya Shimizu

  Springer Science and Business Media LLC, Jun. 2023, Archives of Microbiology, 205(7) (7), English

  [Refereed]

  Scientific journal


• l-Lactate treatment by photosynthetic cyanobacteria expressing heterogeneous l-lactate dehydrogenase

  Yuichi Kato, Kosuke Inabe, Yuji Haraguchi, Tatsuya Shimizu, Akihiko Kondo, Tomohisa Hasunuma

  Abstract l-Lactate is a major waste compound in cultured animal cells. To develop a sustainable animal cell culture system, we aimed to study the consumption of l-lactate using a photosynthetic microorganism. As genes involved in l-lactate utilization were not found in most cyanobacteria and microalgae, we introduced the NAD-independent l-lactate dehydrogenase gene from Escherichia coli (lldD) into Synechococcus sp. PCC 7002. The lldD-expressing strain consumed l-lactate added to basal medium. This consumption was accelerated by expression of a lactate permease gene from E. coli (lldP) and an increase in culture temperature. Intracellular levels of acetyl-CoA, citrate, 2-oxoglutarate, succinate, and malate, and extracellular levels of 2-oxoglutarate, succinate, and malate, increased during l-lactate utilization, suggesting that the metabolic flux from l-lactate was distributed toward the tricarboxylic acid cycle. This study provides a perspective on l-lactate treatment by photosynthetic microorganisms, which would increase the feasibility of animal cell culture industries.

  Springer Science and Business Media LLC, May 2023, Scientific Reports, 13(1) (1), English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Metabolomic analysis of the effect of nitrogen on fucoxanthin synthesis by the haptophyte Pavlova gyrans

  Erina Yoshida, Yuichi Kato, Akihiko Kanamoto, Akihiko Kondo, Tomohisa Hasunuma

  Elsevier BV, May 2023, Algal Research, 72, 103144 - 103144, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Enhanced supply of acetyl-CoA by exogenous pantothenate kinase promotes synthesis of poly(3-hydroxybutyrate)

  Hirotaka Kudo, Sho Ono, Kenta Abe, Mami Matsuda, Tomohisa Hasunuma, Tomoyasu Nishizawa, Munehiko Asayama, Hirofumi Nishihara, Shigeru Chohnan

  Abstract Background Coenzyme A (CoA) is a carrier of acyl groups. This cofactor is synthesized from pantothenic acid in five steps. The phosphorylation of pantothenate is catalyzed by pantothenate kinase (CoaA), which is a key step in the CoA biosynthetic pathway. To determine whether the enhancement of the CoA biosynthetic pathway is effective for producing useful substances, the effect of elevated acetyl-CoA levels resulting from the introduction of the exogenous coaA gene on poly(3-hydroxybutyrate) [P(3HB)] synthesis was determined in Escherichia coli, which express the genes necessary for cyanobacterial polyhydroxyalkanoate synthesis (phaABEC). Results E. coli containing the coaA gene in addition to the pha genes accumulated more P(3HB) compared with the transformant containing the pha genes alone. P(3HB) production was enhanced by precursor addition, with P(3HB) content increasing from 18.4% (w/w) to 29.0% in the presence of 0.5 mM pantothenate and 16.3%–28.2% by adding 0.5 mM β-alanine. Strains expressing the exogenous coaA in the presence of precursors contained acetyl-CoA in excess of 1 nmol/mg of dry cell wt, which promoted the reaction toward P(3HB) formation. The amount of acetate exported into the medium was three times lower in the cells carrying exogenous coaA and pha genes than in the cells carrying pha genes alone. This was attributed to significantly enlarging the intracellular pool size of CoA, which is the recipient of acetic acid and is advantageous for microbial production of value-added materials. Conclusions Enhancing the CoA biosynthetic pathway with exogenous CoaA was effective at increasing P(3HB) production. Supplementing the medium with pantothenate facilitated the accumulation of P(3HB). β-Alanine was able to replace the efficacy of adding pantothenate.

  Springer Science and Business Media LLC, Apr. 2023, Microbial Cell Factories, 22(1) (1), English

  [Refereed]

  Scientific journal


• Skeletal myotube-derived extracellular vesicles enhance itaconate production and attenuate inflammatory responses of macrophages.

  Atomu Yamaguchi, Noriaki Maeshige, Jiawei Yan, Xiaoqi Ma, Mikiko Uemura, Mami Matsuda, Yuya Nishimura, Tomohisa Hasunuma, Hiroyo Kondo, Hidemi Fujino, Zhi-Min Yuan

  INTRODUCTION: Macrophages play an important role in the innate immunity. While macrophage inflammation is necessary for biological defense, it must be appropriately controlled. Extracellular vesicles (EVs) are small vesicles released from all types of cells and play a central role in intercellular communication. Skeletal muscle has been suggested to release anti-inflammatory factors, but the effect of myotube-derived EVs on macrophages is unknown. As an anti-inflammatory mechanism of macrophages, the immune responsive gene 1 (IRG1)-itaconate pathway is essential. In this study, we show that skeletal muscle-derived EVs suppress macrophage inflammatory responses, upregulating the IRG1-itaconate pathway. METHODS: C2C12 myoblasts were differentiated into myotubes and EVs were extracted by ultracentrifugation. Skeletal myotube-derived EVs were administered to mouse bone marrow-derived macrophages, then lipopolysaccharide (LPS) stimulation was performed and inflammatory cytokine expression was measured by RT-qPCR. Metabolite abundance in macrophages after addition of EVs was measured by CE/MS, and IRG1 expression was measured by RT-PCR. Furthermore, RNA-seq analysis was performed on macrophages after EV treatment. RESULTS: EVs attenuated the expression of LPS-induced pro-inflammatory factors in macrophages. Itaconate abundance and IRG1 expression were significantly increased in the EV-treated group. RNA-seq analysis revealed activation of the PI3K-Akt and JAK-STAT pathways in macrophages after EV treatment. The most abundant miRNA in myotube EVs was miR-206-3p, followed by miR-378a-3p, miR-30d-5p, and miR-21a-5p. DISCUSSION: Skeletal myotube EVs are supposed to increase the production of itaconate via upregulation of IRG1 expression and exhibited an anti-inflammatory effect in macrophages. This anti-inflammatory effect was suggested to involve the PI3K-Akt and JAK-STAT pathways. The miRNA profiles within EVs implied that miR-206-3p, miR-378a-3p, miR-30d-5p, and miR-21a-5p may be responsible for the anti-inflammatory effects of the EVs. In summary, in this study we showed that myotube-derived EVs prevent macrophage inflammatory responses by activating the IRG1-itaconate pathway.

  Mar. 2023, Frontiers in immunology, 14, 1099799 - 1099799, English, International magazine

  [Refereed]

  Scientific journal


• Enhanced production of 3,4‐dihydroxybutyrate from xylose by engineered yeast via xylonate re‐assimilation under alkaline condition

  Takahiro Yukawa, Takahiro Bamba, Mami Matsuda, Takanobu Yoshida, Kentaro Inokuma, Jungyeon Kim, Jae Won Lee, Yong‐Su Jin, Akihiko Kondo, Tomohisa Hasunuma

  Wiley, Feb. 2023, Biotechnology and Bioengineering, 120(2) (2), 511 - 523, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Direct production of 4‐hydroxybenzoic acid from cellulose using cellulase‐displaying Pichia pastoris

  Kentaro Inokuma, Shunya Miyamoto, Kohei Morinaga, Yuma Kobayashi, Ryota Kumokita, Takahiro Bamba, Yoichiro Ito, Akihiko Kondo, Tomohisa Hasunuma

  Wiley, Jan. 2023, Biotechnology and Bioengineering, 120(4) (4), 1097 - 1107, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Enhanced growth and lipid productivity by living Chlorella sorokiniana immobilized in Ca-alginate beads

  Daniel A Alfaro-Sayes, Jerome Amoah, Nova Rachmadona, Shinji Hama, Tomohisa Hasunuma, Akihiko Kondo, Chiaki Ogino

  Abstract The bottleneck for the production of biofuels from microalgae consists on costly harvesting processes and low lipid production, immobilization technology could play a part on making the production of biofuels more feasible. The aim of this study was to evaluate the effect of alginate immobilization on the growth and lipid productivity of the microalgae Chlorella sorokiniana, so far, the main focus of immobilization technology has been its use for wastewater treatment and nutrient removal from effluents. The microalgae Chlorella sorokiniana was cultured in both free and immobilized forms under optimal autotrophic growth conditions. Microalgae were immobilized in calcium alginate beads generated by mixing algal cells with a sodium alginate solution, followed by extrusion into a CaCl₂ solution. The results obtained in this study showed that the growth of the microalgae immobilized in alginate beads, was enhanced and achieved a dry cell weight 1.4-fold higher than that of a free cell culture, a higher light transmittance was also achieved in the alginate immobilized culture, and the lipid productivity was increased from 54.21 ± 2.48 mg l⁻¹ d in the free cell culture to 82.22 ± 8.48 mg l⁻¹ d in the immobilized culture. These results demonstrate the effectiveness of immobilization technology for promoting growth and lipid productivity in the microalgae Chlorella sorokiniana.

  IOP Publishing, Jan. 2023, Journal of Physics: Energy, 5(1) (1), 014019 - 014019, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Online SFE-SFC-MS/MS colony screening: A high-throughput approach for optimizing (-)-limonene production

  Musashi Takekana, Takanobu Yoshida, Erika Yoshida, Sumika Ono, Shinnosuke Horie, Christopher J. Vavricka, Moe Hiratani, Kenji Tsuge, Jun Ishii, Yoshihiro Hayakawa, Akihiko Kondo, Tomohisa Hasunuma

  Elsevier BV, Jan. 2023, Journal of Chromatography B, 1215, 123588 - 123588, English

  [Refereed]

  Scientific journal


• Improving methanol assimilation in Yarrowia lipolytica via systematic metabolic engineering combined with compartmentalization

  Shangjie Zhang, Feng Guo, Qiao Yang, Yujia Jiang, Shihui Yang, Jiangfeng Ma, Fengxue Xin, Tomohisa Hasunuma, Akihiko Kondo, Wenming Zhang, Min Jiang

  Synthetic methylotrophic Yarrowia lipolytica was constructed to convert methanol into biomass components and succinic acid.

  Royal Society of Chemistry (RSC), 2023, Green Chemistry, 25(1) (1), 183 - 195, English

  [Refereed]

  Scientific journal


• Dark accumulation of downstream glycolytic intermediates initiates robust photosynthesis in cyanobacteria

  Kenya Tanaka, Tomokazu Shirai, Christopher J Vavricka, Mami Matsuda, Akihiko Kondo, Tomohisa Hasunuma

  Abstract Photosynthesis must maintain stability and robustness throughout fluctuating natural environments. In cyanobacteria, dark-to-light transition leads to drastic metabolic changes from dark respiratory metabolism to CO2 fixation through the Calvin–Benson–Bassham (CBB) cycle using energy and redox equivalents provided by photosynthetic electron transfer. Previous studies have shown that catabolic metabolism supports the smooth transition into CBB cycle metabolism. However, metabolic mechanisms for robust initiation of photosynthesis are poorly understood due to lack of dynamic metabolic characterizations of dark-to-light transitions. Here, we show rapid dynamic changes (on a time scale of seconds) in absolute metabolite concentrations and 13C tracer incorporation after strong or weak light irradiation in the cyanobacterium Synechocystis sp. PCC 6803. Integration of this data enabled estimation of time-resolved nonstationary metabolic flux underlying CBB cycle activation. This dynamic metabolic analysis indicated that downstream glycolytic intermediates, including phosphoglycerate and phosphoenolpyruvate, accumulate under dark conditions as major substrates for initial CO2 fixation. Compared with wild-type Synechocystis, significant decreases in the initial oxygen evolution rate were observed in 12 h dark preincubated mutants deficient in glycogen degradation or oxidative pentose phosphate pathways. Accordingly, the degree of decrease in the initial oxygen evolution rate was proportional to the accumulated pool size of glycolytic intermediates. These observations indicate that the accumulation of glycolytic intermediates is essential for efficient metabolism switching under fluctuating light environments.

  Oxford University Press (OUP), Dec. 2022, Plant Physiology, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Metabolic and Microbial Community Engineering for Four-Carbon Dicarboxylic Acid Production from CO₂-Derived Glycogen in the Cyanobacterium Synechocystis sp. PCC6803

  Ryota Hidese, Mami Matsuda, Mamiko Kajikawa, Takashi Osanai, Akihiko Kondo, Tomohisa Hasunuma

  The four-carbon (C4) dicarboxylic acids, fumarate, malate, and succinate, are the most valuable targets that must be exploited for CO2-based chemical production in the move to a sustainable low-carbon future. Cyanobacteria excrete high amounts of C4 dicarboxylic acids through glycogen fermentation in a dark anoxic environment. The enhancement of metabolic flux in the reductive TCA branch in the Cyanobacterium Synechocystis sp. PCC6803 is a key issue in the C4 dicarboxylic acid production. To improve metabolic flux through the anaplerotic pathway, we have created the recombinant strain PCCK, which expresses foreign ATP-forming phosphoenolpyruvate carboxykinase (PEPck) concurrent with intrinsic phosphoenolpyruvate carboxylase (Ppc) overexpression. Expression of PEPck concurrent with Ppc led to an increase in C4 dicarboxylic acids by autofermentation. Metabolome analysis revealed that PEPck contributed to an increase in carbon flux from hexose and pentose phosphates into the TCA reductive branch. To enhance the metabolic flux in the reductive TCA branch, we examined the effect of corn-steep liquor (CSL) as a nutritional supplement on C4 dicarboxylic acid production. Surprisingly, the addition of sterilized CSL enhanced the malate production in the PCCK strain. Thereafter, the malate and fumarate excreted by the PCCK strain are converted into succinate by the CSL-settling microorganisms. Finally, high-density cultivation of cells lacking the acetate kinase gene showed the highest production of malate and fumarate (3.2 and 2.4 g/L with sterilized CSL) and succinate (5.7 g/L with non-sterile CSL) after 72 h cultivation. The present microbial community engineering is useful for succinate production by one-pot fermentation under dark anoxic conditions.

  American Chemical Society (ACS), Dec. 2022, ACS Synthetic Biology, 11(12) (12), 4054 - 4064, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Alginate immobilization as a strategy for improving succinate production during autofermentation using cyanobacteria Synechocystis sp. PCC 6803

  Daniel A. Alfaro-Sayes, Jerome Amoah, Shimpei Aikawa, Mami Matsuda, Tomohisa Hasunuma, Akihiko Kondo, Chiaki Ogino

  Elsevier BV, Dec. 2022, Biochemical Engineering Journal, 188, 108681 - 108681, English

  [Refereed]

  Scientific journal


• Machine learning discovery of missing links that mediate alternative branches to plant alkaloids

  Christopher J. Vavricka, Shunsuke Takahashi, Naoki Watanabe, Musashi Takenaka, Mami Matsuda, Takanobu Yoshida, Ryo Suzuki, Hiromasa Kiyota, Jianyong Li, Hiromichi Minami, Jun Ishii, Kenji Tsuge, Michihiro Araki, Akihiko Kondo, Tomohisa Hasunuma

  Abstract Engineering the microbial production of secondary metabolites is limited by the known reactions of correctly annotated enzymes. Therefore, the machine learning discovery of specialized enzymes offers great potential to expand the range of biosynthesis pathways. Benzylisoquinoline alkaloid production is a model example of metabolic engineering with potential to revolutionize the paradigm of sustainable biomanufacturing. Existing bacterial studies utilize a norlaudanosoline pathway, whereas plants contain a more stable norcoclaurine pathway, which is exploited in yeast. However, committed aromatic precursors are still produced using microbial enzymes that remain elusive in plants, and additional downstream missing links remain hidden within highly duplicated plant gene families. In the current study, machine learning is applied to predict and select plant missing link enzymes from homologous candidate sequences. Metabolomics-based characterization of the selected sequences reveals potential aromatic acetaldehyde synthases and phenylpyruvate decarboxylases in reconstructed plant gene-only benzylisoquinoline alkaloid pathways from tyrosine. Synergistic application of the aryl acetaldehyde producing enzymes results in enhanced benzylisoquinoline alkaloid production through hybrid norcoclaurine and norlaudanosoline pathways.

  Springer Science and Business Media LLC, Dec. 2022, Nature Communications, 13(1) (1), 1405 - 1405, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Development of a stable semi-continuous lipid production system of an oleaginous Chlamydomonas sp. mutant using multi-omics profiling

  Tomoki Oyama, Yuichi Kato, Ryota Hidese, Mami Matsuda, Minenosuke Matsutani, Satoru Watanabe, Akihiko Kondo, Tomohisa Hasunuma

  Abstract Background Microalgal lipid production has attracted global attention in next-generation biofuel research. Nitrogen starvation, which drastically suppresses cell growth, is a common and strong trigger for lipid accumulation in microalgae. We previously developed a mutant Chlamydomonas sp. KAC1801, which can accumulate lipids irrespective of the presence or absence of nitrates. This study aimed to develop a feasible strategy for stable and continuous lipid production through semi-continuous culture of KAC1801. Results KAC1801 continuously accumulated > 20% lipid throughout the subculture (five generations) when inoculated with a dry cell weight of 0.8–0.9 g L⁻¹ and cultured in a medium containing 18.7 mM nitrate, whereas the parent strain KOR1 accumulated only 9% lipid. Under these conditions, KAC1801 continuously produced biomass and consumed nitrates. Lipid productivity of 116.9 mg L⁻¹ day⁻¹ was achieved by semi-continuous cultivation of KAC1801, which was 2.3-fold higher than that of KOR1 (50.5 mg L⁻¹ day⁻¹). Metabolome and transcriptome analyses revealed a depression in photosynthesis and activation of nitrogen assimilation in KAC1801, which are the typical phenotypes of microalgae under nitrogen starvation. Conclusions By optimizing nitrate supply and cell density, a one-step cultivation system for Chlamydomonas sp. KAC1801 under nitrate-replete conditions was successfully developed. KAC1801 achieved a lipid productivity comparable to previously reported levels under nitrogen-limiting conditions. In the culture system of this study, metabolome and transcriptome analyses revealed a nitrogen starvation-like response in KAC1801.

  Springer Science and Business Media LLC, Sep. 2022, Biotechnology for Biofuels and Bioproducts, 15(1) (1), English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Improvement of cell-tethered cellulase activity in recombinant strains of Saccharomyces cerevisiae

  Bronwyn Jean Chetty, Kentaro Inokuma, Tomohisa Hasunuma, Willem Heber van Zyl, Riaan den Haan

  Springer Science and Business Media LLC, Sep. 2022, Applied Microbiology and Biotechnology, 106(18) (18), 6347 - 6361, English

  [Refereed]

  Scientific journal


• Avoiding entry into intracellular protein degradation pathways by signal mutations increases protein secretion in Pichia pastoris

  Yoichiro Ito, Misa Ishigami, Noriko Hashiba, Yasuyuki Nakamura, Goro Terai, Tomohisa Hasunuma, Jun Ishii, Akihiko Kondo

  Wiley, Sep. 2022, Microbial Biotechnology, 15(9) (9), 2364 - 2378, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Metabolic engineering of the L-serine biosynthetic pathway improves glutathione production in Saccharomyces cerevisiae.

  Jyumpei Kobayashi, Daisuke Sasaki, Kiyotaka Y Hara, Tomohisa Hasunuma, Akihiko Kondo

  BACKGROUND: Glutathione is a valuable tri-peptide that is industrially produced by fermentation using the yeast Saccharomyces cerevisiae, and is widely used in the pharmaceutical, food, and cosmetic industries. It has been reported that addition of L-serine (L-Ser) is effective at increasing the intracellular glutathione content because L-Ser is the common precursor of L-cysteine (L-Cys) and glycine (Gly) which are substrates for glutathione biosynthesis. Therefore, we tried to enhance the L-Ser biosynthetic pathway in S. cerevisiae for improved glutathione production. RESULTS: The volumetric glutathione production of recombinant strains individually overexpressing SER2, SER1, SER3, and SER33 involved in L-Ser biosynthesis at 48 h cultivation was increased 1.3, 1.4, 1.9, and 1.9-fold, respectively, compared with that of the host GCI strain, which overexpresses genes involved in glutathione biosynthesis. We further examined simultaneous overexpression of SHM2 and/or CYS4 genes involved in Gly and L-Cys biosynthesis, respectively, using recombinant GCI strain overexpressing SER3 and SER33 as hosts. As a result, GCI overexpressing SER3, SHM2, and CYS4 showed the highest volumetric glutathione production (64.0 ± 4.9 mg/L) at 48 h cultivation, and this value is about 2.5-fold higher than that of the control strain. CONCLUSIONS: This study first revealed that engineering of L-Ser and Gly biosynthetic pathway are useful strategies for fermentative glutathione production by S. cerevisiase.

  Springer Science and Business Media LLC, Aug. 2022, Microbial cell factories, 21(1) (1), 153 - 153, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Construction of an l-Tyrosine Chassis in Pichia pastoris Enhances Aromatic Secondary Metabolite Production from Glycerol

  Ryota Kumokita, Takahiro Bamba, Kentaro Inokuma, Takanobu Yoshida, Yoichiro Ito, Akihiko Kondo, Tomohisa Hasunuma

  American Chemical Society (ACS), Jun. 2022, ACS Synthetic Biology, 11(6) (6), 2098 - 2107, English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella phaffii

  Yoichiro Ito, Misa Ishigami, Goro Terai, Yasuyuki Nakamura, Noriko Hashiba, Teruyuki Nishi, Hikaru Nakazawa, Tomohisa Hasunuma, Kiyoshi Asai, Mitsuo Umetsu, Jun Ishii, Akihiko Kondo

  Abstract Expression of secreted recombinant proteins burdens the protein secretion machinery, limiting production. Here, we describe an approach to improving protein production by the non-conventional yeast Komagataella phaffii comprised of genome-wide screening for effective gene disruptions, combining them in a single strain, and recovering growth reduction by adaptive evolution. For the screen, we designed a multiwell-formatted, streamlined workflow to high-throughput assay of secretion of a single-chain small antibody, which is cumbersome to detect but serves as a good model of proteins that are difficult to secrete. Using the consolidated screening system, we evaluated >19,000 mutant strains from a mutant library prepared by a modified random gene-disruption method, and identified six factors for which disruption led to increased antibody production. We then combined the disruptions, up to quadruple gene knockouts, which appeared to contribute independently, in a single strain and observed an additive effect. Target protein and promoter were basically interchangeable for the effects of knockout genes screened. We finally used adaptive evolution to recover reduced cell growth by multiple gene knockouts and examine the possibility for further enhancing protein secretion. Our successful, three-part approach holds promise as a method for improving protein production by non-conventional microorganisms.

  Springer Science and Business Media LLC, Jun. 2022, Communications Biology, 5(1) (1), 561, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Accumulated 3-phosphoglycerate in darkness is a primary starting substrate for stepwise photosynthetic induction process in cyanobacteria

  Kenya Tanaka, Tomokazu Shirai, Mami Matsuda, Akihiko Kondo, Tomohisa Hasunuma

  Cold Spring Harbor Laboratory, Apr. 2022

  Scientific journal


• Pulsed-Ultrasound Irradiation Induces the Production of Itaconate and Attenuates Inflammatory Responses in Macrophages

  Atomu Yamaguchi, Noriaki Maeshige, Xiaoqi Ma, Mikiko Uemura, Hikari Noguchi, Mami Matsuda, Yuya Nishimura, Tomohisa Hasunuma, Hiroyo Kondo, Hidemi Fujino

  Background: Itaconate is a key metabolite in the innate immune system and exerts strong anti-inflammatory effects in macrophages. For the production of itaconate in macrophages, immune-responsive gene 1 (IRG1) is an imperative enzyme, and activating the IRG1-itaconate pathway is reported to alleviate inflammatory diseases by upregulating nuclear factor-erythroid 2-related factor 2 (NRF2). However, there are very few reports on strategies to increase itaconate production. Ultrasound therapy is a widely used intervention for anti-inflammatory and soft-tissue regeneration purposes. Here we show the effect of ultrasound irradiation on the production of itaconate in macrophages. Methods: Murine bone marrow-derived macrophages (BMDMs) were exposed to pulsed ultrasound (3.0 W/cm2) for 5 minutes. Three hours after irradiation, the intracellular levels of metabolites and mRNA expression levels of Irg1 and Nrf2 were measured using CE/MS and qPCR, respectively. To evaluate macrophage inflammation status, 3 h after irradiation, the cells were stimulated with 100 ng/mL lipopolysaccharide (LPS) for 1.5 h and the mRNA expression levels of pro-inflammatory factors (Il-1β, Il-6, and Tnf-α) were measured. Student's t-test, one-way ANOVA and Tukey's multiple comparison test were used for statistical processing, and the significance level was set to less than 5%. Results: Ultrasound irradiation significantly increased the intracellular itaconate level and the expression levels of Irg1 and Nrf2 in BMDMs. Upregulation of Il-1β, Il-6, and Tnf-α by LPS was significantly suppressed in BMDMs treated with ultrasound. Ultrasound irradiation did not affect cell viability and apoptosis. Conclusion: Ultrasound irradiation induces the production of itaconate by upregulating Irg1 expression and attenuates inflammatory responses in macrophages via Nrf2. These results suggest that ultrasound is a potentially useful method to increase itaconate production in macrophages.

  Informa UK Limited, Apr. 2022, Journal of Inflammation Research, Volume 15, 2387 - 2395, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Enzyme display technology for lignocellulosic biomass valorization by yeast cell factories

  Takahiro Bamba, Gregory Guirimand, Akihiko Kondo, Tomohisa Hasunuma

  Elsevier BV, Feb. 2022, Current Opinion in Green and Sustainable Chemistry, 33, 100584 - 100584, English

  Scientific journal


• Efficient preparation of soluble inducer for cellulase production and saccharification of corn stover using in-house generated crude enzymes

  Yonghao Li, Peng Zhang, Deying Zhu, Bo Yao, Tomohisa Hasunuma, Akihiko Kondo, Xinqing Zhao

  Elsevier BV, Jan. 2022, Biochemical Engineering Journal, 178, 108296 - 108296

  Scientific journal


• Improved ethanol fermentation by promoter replacement of zinc responsive genes IPL1, PRP6 and RTC1 in Saccharomyces cerevisiae

  Hong-Qi Chen, Ming-Ming Zhang, Qi Xing, Pei-Liang Ye, Tomohisa Hasunuma, Akihiko Kondo, Xin-Qing Zhao

  Elsevier BV, Jan. 2022, Biochemical Engineering Journal, 178, 108274 - 108274

  Scientific journal


• Resveratrol production of a recombinant Scheffersomyces stipitis strain from molasses

  Yuma Kobayashi, Kentaro Inokuma, Mami Matsuda, Akihiko Kondo, Tomohisa Hasunuma

  Elsevier BV, Jan. 2022, Biotechnology Notes, 3, 1 - 7, English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Metabolomics-based engineering for biofuel and bio-based chemical production in microalgae and cyanobacteria: A review

  Yuichi Kato, Kosuke Inabe, Ryota Hidese, Akihiko Kondo, Tomohisa Hasunuma

  Metabolomics, an essential tool in modern synthetic biology based on the design-build-test-learn platform, is useful for obtaining a detailed understanding of cellular metabolic mechanisms through comprehensive analyses of the metabolite pool size and its dynamic changes. Metabolomics is critical to the design of a rational metabolic engineering strategy by determining the rate-limiting reaction and assimilated carbon distribution in a biosynthetic pathway of interest. Microalgae and cyanobacteria are promising photosynthetic producers of biofuels and bio-based chemicals, with high potential for developing a bioeconomic society through bio-based carbon neutral manufacturing. Metabolomics technologies optimized for photosynthetic organisms have been developed and utilized in various microalgal and cyanobacterial species. This review provides a concise overview of recent achievements in photosynthetic metabolomics, emphasizing the importance of microalgal and cyanobacterial cell factories that satisfy industrial requirements.

  Elsevier BV, Jan. 2022, Bioresource Technology, 344(Pt A) (Pt A), 126196 - 126196, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Pretreatment of extruded Napier grass by hydrothermal process with dilute sulfuric acid and fermentation using a cellulose-hydrolyzing and xylose-assimilating yeast for ethanol production

  Ku Syahidah Ku Ismail, Yuki Matano, Yuri Sakihama, Kentaro Inokuma, Yumiko Nambu, Tomohisa Hasunuma, Akihiko Kondo

  One of the potential bioresources for bioethanol production is Napier grass, considering its high cellulose and hemicellulose content. However, the cost of pretreatment hinders the bioethanol produced from being economical. This study examines the effect of hydrothermal process with dilute acid on extruded Napier grass, followed by enzymatic saccharification prior to simultaneous saccharification and co-fermentation (SScF). Extrusion facilitated lignin removal by 30.2 % prior to dilute acid steam explosion. Optimum pretreatment condition was obtained by using 3% sulfuric acid, and 30-min retention time of steam explosion at 190 °C. Ethanol yield of 0.26 g ethanol/g biomass (60.5% fermentation efficiency) was attained by short-term liquefaction and fermentation using a cellulose-hydrolyzing and xylose-assimilating Saccharomyces cerevisiae NBRC1440/B-EC3-X ΔPHO13, despite the presence of inhibitors. This proposed method not only reduced over-degradation of cellulose and hemicellulose, but also eliminated detoxification process and reduced cellulase loading.

  Elsevier BV, Jan. 2022, Bioresource Technology, 343, 126071 - 126071, English, International magazine

  Scientific journal


• Nitrogen Availability Affects the Metabolic Profile in Cyanobacteria

  Kosuke Inabe, Ayaka Miichi, Mami Matsuda, Takanobu Yoshida, Yuichi Kato, Ryota Hidese, Akihiko Kondo, Tomohisa Hasunuma

  Nitrogen is essential for the biosynthesis of various molecules in cells, such as amino acids and nucleotides, as well as several types of lipids and sugars. Cyanobacteria can assimilate several forms of nitrogen, including nitrate, ammonium, and urea, and the physiological and genetic responses to these nitrogen sources have been studied previously. However, the metabolic changes in cyanobacteria caused by different nitrogen sources have not yet been characterized. This study aimed to elucidate the influence of nitrate and ammonium on the metabolic profiles of the cyanobacterium Synechocystis sp. strain PCC 6803. When supplemented with NaNO3 or NH4Cl as the nitrogen source, Synechocystis sp. PCC 6803 grew faster in NH4Cl medium than in NaNO3 medium. Metabolome analysis indicated that some metabolites in the CBB cycle, glycolysis, and TCA cycle, and amino acids were more abundant when grown in NH4Cl medium than NaNO3 medium. 15N turnover rate analysis revealed that the nitrogen assimilation rate in NH4Cl medium was higher than in NaNO3 medium. These results indicate that the mechanism of nitrogen assimilation in the GS-GOGAT cycle differs between NaNO3 and NH4Cl. We conclude that the amounts and biosynthetic rate of cyanobacterial metabolites varies depending on the type of nitrogen.

  MDPI AG, Dec. 2021, Metabolites, 11(12) (12), 867 - 867, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Enhanced production of γ-amino acid 3-amino-4-hydroxybenzoic acid by recombinant Corynebacterium glutamicum under oxygen limitation

  Hideo Kawaguchi, Tomohisa Hasunuma, Yasuo Ohnishi, Takashi Sazuka, Akihiko Kondo, Chiaki Ogino

  Bio-based aromatic compounds are of great interest to the industry, as commercial production of aromatic compounds depends exclusively on the unsustainable use of fossil resources or extraction from plant resources. γ-amino acid 3-amino-4-hydroxybenzoic acid (3,4-AHBA) serves as a precursor for thermostable bioplastics. Under aerobic conditions, a recombinant Corynebacterium glutamicum strain KT01 expressing griH and griI genes derived from Streptomyces griseus produced 3,4-AHBA with large amounts of amino acids as by-products. The specific productivity of 3,4-AHBA increased with decreasing levels of dissolved oxygen (DO) and was eightfold higher under oxygen limitation (DO = 0 ppm) than under aerobic conditions (DO ≥ 2.6 ppm). Metabolic profiles during 3,4-AHBA production were compared at three different DO levels (0, 2.6, and 5.3 ppm) using the DO-stat method. Results of the metabolome analysis revealed metabolic shifts in both the central metabolic pathway and amino acid metabolism at a DO of < 33% saturated oxygen. Based on this metabolome analysis, metabolic pathways were rationally designed for oxygen limitation. An ldh deletion mutant, with the loss of lactate dehydrogenase, exhibited 3.7-fold higher specific productivity of 3,4-AHBA at DO = 0 ppm as compared to the parent strain KT01 and produced 5.6 g/L 3,4-AHBA in a glucose fed-batch culture. Our results revealed changes in the metabolic state in response to DO concentration and provided insights into oxygen supply during fermentation and the rational design of metabolic pathways for improved production of related amino acids and their derivatives.

  Springer Science and Business Media LLC, Dec. 2021, Microbial Cell Factories, 20(1) (1), 228, English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Development of mutant microalgae that accumulate lipids under nitrate-replete conditions

  Tomoki Oyama, Yuichi Kato, Katsuya Satoh, Yutaka Oono, Mami Matsuda, Tomohisa Hasunuma, Akihiko Kondo

  Elsevier BV, Dec. 2021, Algal Research, 60, 102544 - 102544

  Scientific journal


• Resveratrol production from several types of saccharide sources by a recombinant Scheffersomyces stipitis strain

  Yuma Kobayashi, Kentaro Inokuma, Mami Matsuda, Akihiko Kondo, Tomohisa Hasunuma

  Elsevier BV, Dec. 2021, Metabolic Engineering Communications, 13, e00188 - e00188, English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Disruption of alpha-tubulin releases carbon catabolite repression and enhances enzyme production in Trichoderma reesei even in the presence of glucose

  Nozomu Shibata, Hiroshi Kakeshita, Kazuaki Igarashi, Yasushi Takimura, Yosuke Shida, Wataru Ogasawara, Tohru Koda, Tomohisa Hasunuma, Akihiko Kondo

  Trichoderma reesei is a filamentous fungus that is important as an industrial producer of cellulases and hemicellulases due to its high secretion of these enzymes and outstanding performance in industrial fermenters. However, the reduction of enzyme production caused by carbon catabolite repression (CCR) has long been a problem. Disruption of a typical transcriptional regulator, Cre1, does not sufficiently suppress this reduction in the presence of glucose. We found that deletion of an α-tubulin (tubB) in T. reesei enhanced both the amount and rate of secretory protein production. Also, the tubulin-disrupted (ΔtubB) strain had high enzyme production and the same enzyme profile even if the strain was cultured in a glucose-containing medium. From transcriptome analysis, the ΔtubB strain exhibited upregulation of both cellulase and hemicellulase genes including some that were not originally induced by cellulose. Moreover, cellobiose transporter genes and the other sugar transporter genes were highly upregulated, and simultaneous uptake of glucose and cellobiose was also observed in the ΔtubB strain. These results suggested that the ΔtubB strain was released from CCR. Trichoderma reesei α-tubulin is involved in the transcription of cellulase and hemicellulase genes, as well as in CCR. This is the first report of overcoming CCR by disrupting α-tubulin gene in T. reesei. The disruption of α-tubulin is a promising approach for creating next-generation enzyme-producing strains of T. reesei.

  Springer Science and Business Media LLC, Dec. 2021, Biotechnology for Biofuels, 14(1) (1), 39 - 39, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Enhancing carbohydrate repartitioning into lipid and carotenoid by disruption of microalgae starch debranching enzyme

  Yuichi Kato, Tomoki Oyama, Kentaro Inokuma, Christopher J. Vavricka, Mami Matsuda, Ryota Hidese, Katsuya Satoh, Yutaka Oono, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo

  Light/dark cycling is an inherent condition of outdoor microalgae cultivation, but is often unfavorable for lipid accumulation. This study aims to identify promising targets for metabolic engineering of improved lipid accumulation under outdoor conditions. Consequently, the lipid-rich mutant Chlamydomonas sp. KOR1 was developed through light/dark-conditioned screening. During dark periods with depressed CO₂ fixation, KOR1 shows rapid carbohydrate degradation together with increased lipid and carotenoid contents. KOR1 was subsequently characterized with extensive mutation of the ISA1 gene encoding a starch debranching enzyme (DBE). Dynamic time-course profiling and metabolomics reveal dramatic changes in KOR1 metabolism throughout light/dark cycles. During light periods, increased flux from CO₂ through glycolytic intermediates is directly observed to accompany enhanced formation of small starch-like particles, which are then efficiently repartitioned in the next dark cycle. This study demonstrates that disruption of DBE can improve biofuel production under light/dark conditions, through accelerated carbohydrate repartitioning into lipid and carotenoid.

  Springer Science and Business Media LLC, Dec. 2021, Communications Biology, 4(1) (1), 450 - 450, English, International magazine

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Salinity-induced microalgal-based mariculture wastewater treatment combined with biodiesel production

  Chaofan Zhang, Tomohisa Hasunuma, Su Shiung Lam, Akihiko Kondo, Shih-Hsin Ho

  Elsevier BV, Nov. 2021, Bioresource Technology, 340, 125638 - 125638, English

  Scientific journal


• Cell surface engineering of Saccharomyces cerevisiae for simultaneous valorization of corn cob and cheese whey via ethanol production

  Joana T. Cunha, Daniel G. Gomes, Aloia Romaní, Kentaro Inokuma, Tomohisa Hasunuma, Akihiko Kondo, Lucília Domingues

  Elsevier BV, Sep. 2021, Energy Conversion and Management, 243, 114359 - 114359, English

  Scientific journal


• Improving the functionality of surface-engineered yeast cells by altering the cell wall morphology of the host strain

  Kentaro Inokuma, Yuki Kitada, Takahiro Bamba, Yuma Kobayashi, Takahiro Yukawa, Riaan den Haan, Willem Heber van Zyl, Akihiko Kondo, Tomohisa Hasunuma

  Springer Science and Business Media LLC, Aug. 2021, Applied Microbiology and Biotechnology, 105(14-15) (14-15), 5895 - 5904, English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Lutein production with Chlorella sorokiniana MB-1-M12 using novel two-stage cultivation strategies – metabolic analysis and process improvement

  Jih-Heng Chen, Yuichi Kato, Mami Matsuda, Chun-Yen Chen, Dillirani Nagarajan, Tomohisa Hasunuma, Akihiko Kondo, Jo-Shu Chang

  Microalgae-derived carotenoids have increasingly been considered as feasible green alternatives for synthetic antioxidants. In this study, the lutein high-yielding strain (Chlorella sorokiniana MB-1; henceforth MB-1) and its mutant derivative (C. sorokiniana MB-1-M12; henceforth M12) were evaluated for their growth, biomass production, and lutein accumulation in three different cultivation modes - photoautotrophy, mixotrophy, and heterotrophy. M12 could grow effectively under heterotrophic conditions, but the lutein content was lower, indicating the necessity of photo-induction for lutein accumulation. Metabolic analysis of MB-1 and M12 in autotrophic growth in the presence of carbon dioxide indicated that carbon assimilation and channeling of the fixed metabolites towards carotenoid accumulation was elevated in M12 compared to MB-1. Novel two-stage alternative cultivation strategies (Autotrophic/Heterotrophic and Mixotrophic/Heterotrophic cultures) were applied for enhancing lutein production in M12. Maximum lutein quantity (6.17 mg/g) and production (33.64 mg/L) were obtained with the TSHM strategy that is considered the best two-stage operation.

  Elsevier BV, Aug. 2021, Bioresource Technology, 334, 125200 - 125200, English, International magazine

  Scientific journal


• AI on A CHIP for IDENTIFYING MICROALGAL CELLS with HIGH HEAVY METAL REMOVAL EFFICIENCY

  Muzhen Xu, Jeffrey Harmon, Tomohisa Hasunuma, Akihiro Isozaki, Keisuke Goda

  Institute of Electrical and Electronics Engineers Inc., Jun. 2021, 21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021, 385 - 388, English

  International conference proceedings


• Morphological Indicator for Directed Evolution of Euglena gracilis with a High Heavy Metal Removal Efficiency

  Muzhen Xu, Jeffrey Harmon, Dan Yuan, Sheng Yan, Cheng Lei, Kotaro Hiramatsu, Yuqi Zhou, Mun Hong Loo, Tomohisa Hasunuma, Akihiro Isozaki, Keisuke Goda

  In the past few decades, microalgae-based bioremediation methods for treating heavy metal (HM)-polluted wastewater have attracted much attention by virtue of their environment friendliness, cost efficiency, and sustainability. However, their HM removal efficiency is far from practical use. Directed evolution is expected to be effective for developing microalgae with a much higher HM removal efficiency, but there is no non-invasive or label-free indicator to identify them. Here, we present an intelligent cellular morphological indicator for identifying the HM removal efficiency of Euglena gracilis in a non-invasive and label-free manner. Specifically, we show a strong monotonic correlation (Spearman's ρ = -0.82, P = 2.1 × 10-5) between a morphological meta-feature recognized via our machine learning algorithms and the Cu2+ removal efficiency of 19 E. gracilis clones. Our findings firmly suggest that the morphology of E. gracilis cells can serve as an effective HM removal efficiency indicator and hence have great potential, when combined with a high-throughput image-activated cell sorter, for directed-evolution-based development of E. gracilis with an extremely high HM removal efficiency for practical wastewater treatment worldwide.

  American Chemical Society (ACS), Jun. 2021, Environmental Science & Technology, 55(12) (12), 7880 - 7889, English, International magazine

  Scientific journal


• Synthetic production of prenylated naringenins in yeast using promiscuous microbial prenyltransferases

  Shota Isogai, Nobuyuki Okahashi, Ririka Asama, Tomomi Nakamura, Tomohisa Hasunuma, Fumio Matsuda, Jun Ishii, Akihiko Kondo

  Reconstitution of prenylflavonoids using the flavonoid biosynthetic pathway and prenyltransferases (PTs) in microbes can be a promising attractive alternative to plant-based production or chemical synthesis. Here, we demonstrate that promiscuous microbial PTs can be a substitute for regiospecific but mostly unidentified botanical PTs. To test the prenylations of naringenin, we constructed a yeast strain capable of producing naringenin from l-phenylalanine by genomic integration of six exogenous genes encoding components of the naringenin biosynthetic pathway. Using this platform strain, various microbial PTs were tested for prenylnaringenin production. In vitro screening demonstrated that the fungal AnaPT (a member of the tryptophan dimethylallyltransferase family) specifically catalyzed C-3' prenylation of naringenin, whereas SfN8DT-1, a botanical PT, specifically catalyzed C-8 prenylation. In vivo, the naringenin-producing strain expressing the microbial AnaPT exhibited heterologous microbial production of 3'-prenylnaringenin (3'-PN), in contrast to the previously reported in vivo production of 8-prenylnaringenin (8-PN) using the botanical SfN8DT-1. These findings provide strategies towards expanding the production of a variety of prenylated compounds, including well-known prenylnaringenins and novel prenylflavonoids. These results also suggest the opportunity for substituting botanical PTs, both known and unidentified, that display relatively strict regiospecificity of the prenyl group transfer.

  Elsevier BV, Jun. 2021, Metabolic Engineering Communications, 12, e00169 - e00169, English, International magazine

  Scientific journal


• Innovative Tools and Strategies for Optimizing Yeast Cell Factories

  Gregory Guirimand, Natalja Kulagina, Nicolas Papon, Tomohisa Hasunuma, Vincent Courdavault

  Elsevier BV, May 2021, Trends in Biotechnology, 39(5) (5), 488 - 504, English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Multiple mutations in RNA polymerase β-subunit gene (rpoB) in Streptomyces incarnatus NRRL8089 enhance production of antiviral antibiotic sinefungin: modeling rif cluster region by density functional theory

  Saori Ogawa, Hitomi Shimidzu, Koji Fukuda, Naoki Tsunekawa, Toshiyuki Hirano, Fumitoshi Sato, Kei Yura, Tomohisa Hasunuma, Kozo Ochi, Michio Yamamoto, Wataru Sakamoto, Kentaro Hashimoto, Hiroyuki Ogata, Tadayoshi Kanao, Michiko Nemoto, Kenji Inagaki, Takashi Tamura

  Streptomyces incarnatus NRRL8089 produces the antiviral, antifungal, antiprotozoal nucleoside antibiotic sinefungin. To enhance sinefungin production, multiple mutations were introduced to the rpoB gene encoding RNA polymerase (RNAP) β-subunit at the target residues, D447, S453, H457, and R460. Sparse regression analysis using elastic-net lasso-ridge penalties on previously reported H457X mutations identified a numeric parameter set, which suggested that H457R/Y/F may cause production enhancement. H457R/R460C mutation successfully enhanced the sinefungin production by 3-fold, while other groups of mutations, such as D447G/R460C or D447G/H457Y, made moderate or even negative effects. To identify why the rif cluster residues have diverse effects on sinefungin production, an RNAP/DNA/mRNA complex model was constructed by homology modeling and molecular dynamics simulation. The 4 residues were located near the mRNA strand. Density functional theory–based calculation suggested that D447, H457, and R460 are in direct contact with ribonucleotide, and partially positive charges are induced by negatively charged chain of mRNA.

  Oxford University Press (OUP), Apr. 2021, Bioscience, Biotechnology, and Biochemistry, 85(5) (5), 1275 - 1282, English, International magazine

  Scientific journal


• Development of a Method for Fucoxanthin Production Using the Haptophyte Marine Microalga Pavlova sp. OPMS 30543.

  Akihiko Kanamoto, Yuichi Kato, Erina Yoshida, Tomohisa Hasunuma, Akihiko Kondo

  The natural pigment fucoxanthin has attracted global attention because of its superior antioxidant properties. The haptophyte marine microalgae Pavlova spp. are assumed to be promising industrial fucoxanthin producers as their lack of a cell wall could facilitate the commercialization of cultured cells as a whole food. This study screened promising Pavlova strains with high fucoxanthin content to develop an outdoor cultivation method for fucoxanthin production. Initial laboratory investigations of P. pinguis NBRC 102807, P. lutheri NBRC 102808, and Pavlova sp. OPMS 30543 identified OPMS 30543 as having the highest fucoxanthin content. The culture conditions were optimized for OPMS 30543. Compared with f/2 and Walne's media, the use of Daigo's IMK medium led to the highest biomass production and highest fucoxanthin accumulation. The presence of seawater elements in Daigo's IMK medium was necessary for the growth of OPMS 30543. OPMS 30543 was then cultured outdoors using acrylic pipe photobioreactors, a plastic bag, an open tank, and a raceway pond. Acrylic pipe photobioreactors with small diameters enabled the highest biomass production. Using an acrylic pipe photobioreactor with 60-mm diameter, a fucoxanthin productivity of 4.88 mg/L/day was achieved in outdoor cultivation. Thus, this study demonstrated the usefulness of Pavlova sp. OPMS 30543 for fucoxanthin production in outdoor cultivation.

  Apr. 2021, Marine biotechnology (New York, N.Y.), 23(2) (2), 331 - 341, English, International magazine

  Scientific journal


• Metabolic Engineering for Carotenoid Production Using Eukaryotic Microalgae and Prokaryotic Cyanobacteria.

  Yuichi Kato, Tomohisa Hasunuma

  Eukaryotic microalgae and prokaryotic cyanobacteria are diverse photosynthetic organisms that produce various useful compounds. Due to their rapid growth and efficient biomass production from carbon dioxide and solar energy, microalgae and cyanobacteria are expected to become cost-effective, sustainable bioresources in the future. These organisms also abundantly produce various carotenoids, but further improvement in carotenoid productivity is needed for a successful commercialization. Metabolic engineering via genetic manipulation and mutational breeding is a powerful tool for generating carotenoid-rich strains. This chapter focuses on carotenoid production in microalgae and cyanobacteria, as well as strategies and potential target genes for metabolic engineering. Recent achievements in metabolic engineering that improved carotenoid production in microalgae and cyanobacteria are also reviewed.

  2021, Advances in experimental medicine and biology, 1261, 121 - 135, English, International magazine

  Scientific journal


• Optimization of 1,2,4‐butanetriol production from xylose in Saccharomyces cerevisiae by metabolic engineering of NADH/NADPH balance

  Takahiro Yukawa, Takahiro Bamba, Gregory Guirimand, Mami Matsuda, Tomohisa Hasunuma, Akihiko Kondo

  Wiley, Jan. 2021, Biotechnology and Bioengineering, 118(1) (1), 175 - 185, English

  Scientific journal


• An ion-pair free LC-MS/MS method for quantitative metabolite profiling of microbial bioproduction systems

  Musashi Takenaka, Takanobu Yoshida, Yoshimi Hori, Takahiro Bamba, Masao Mochizuki, Christopher J. Vavricka, Takanari Hattori, Yoshihiro Hayakawa, Tomohisa Hasunuma, Akihiko Kondo

  Elsevier BV, Jan. 2021, Talanta, 222, 121625 - 121625, English

  Scientific journal


• Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression

  Yoichiro Ito, Goro Terai, Misa Ishigami, Noriko Hashiba, Yasuyuki Nakamura, Takahiro Bamba, Ryota Kumokita, Tomohisa Hasunuma, Kiyoshi Asai, Jun Ishii, Akihiko Kondo

  In the yeast Saccharomyces cerevisiae, terminator sequences not only terminate transcription but also affect expression levels of the protein-encoded upstream of the terminator. The non-conventional yeast Pichia pastoris (syn. Komagataella phaffii) has frequently been used as a platform for metabolic engineering but knowledge regarding P. pastoris terminators is limited. To explore terminator sequences available to tune protein expression levels in P. pastoris, we created a ‘terminator catalog’ by testing 72 sequences, including terminators from S. cerevisiae or P. pastoris and synthetic terminators. Altogether, we found that the terminators have a tunable range of 17-fold. We also found that S. cerevisiae terminator sequences maintain function when transferred to P. pastoris. Successful tuning of protein expression levels was shown not only for the reporter gene used to define the catalog but also using betaxanthin production as an example application in pathway flux regulation. Moreover, we found experimental evidence that protein expression levels result from mRNA abundance and in silico evidence that levels reflect the stability of mRNA 3′-UTR secondary structure. In combination with promoter selection, the novel terminator catalog constitutes a basic toolbox for tuning protein expression levels in metabolic engineering and synthetic biology in P. pastoris.

  Oxford University Press (OUP), Dec. 2020, Nucleic Acids Research, 48(22) (22), 13000 - 13012, English

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Japan Computational Mass Spectrometry Meeting 2020 Activity Report

  Hiroyuki Yamamoto, Eisuke Hayakawa, Hiroshi Tsugawa, Yuki Moriya, Eiichiro Fukusaki, Susumu Goto, Tomohisa Hasunuma, Nobuaki Miura, Akiyasu C. Yoshizawa

  The Japan Computational Mass Spectrometry (JCompMS) group was launched in 2016 as a special interest group of the Japanese Society for Bioinformatics (JSBi). It aims to facilitate research communications, protocol exchanges, and further developments of computational mass spectrometry (compMS) in Japan. JCompMS aims to organize data science projects in multi-omics utilizing mass spectrometry such as proteomics, glycomics, lipidomics and metabolomics; currently, its main activities include organizing symposia, workshops, lectures, and hackathons related to compMS research. In this report, we introduce the activities held in 2020 and review the current informatics research on omics using mass spectrometry data in Japan.

  Japanese Proteomics Society, Dec. 2020, Journal of Proteome Data and Methods, 2(0005) (0005), 5, English

  Scientific journal


• Raman image-activated cell sorting

  Nao Nitta, Takanori Iino, Akihiro Isozaki, Mai Yamagishi, Yasutaka Kitahama, Shinya Sakuma, Yuta Suzuki, Hiroshi Tezuka, Minoru Oikawa, Fumihito Arai, Takuya Asai, Dinghuan Deng, Hideya Fukuzawa, Misa Hase, Tomohisa Hasunuma, Takeshi Hayakawa, Kei Hiraki, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Yuki Inoue, Takuro Ito, Masataka Kajikawa, Hiroshi Karakawa, Yusuke Kasai, Yuichi Kato, Hirofumi Kobayashi, Cheng Lei, Satoshi Matsusaka, Hideharu Mikami, Atsuhiro Nakagawa, Keiji Numata, Tadataka Ota, Takeichiro Sekiya, Kiyotaka Shiba, Yoshitaka Shirasaki, Nobutake Suzuki, Shunji Tanaka, Shunnosuke Ueno, Hiroshi Watarai, Takashi Yamano, Masayuki Yazawa, Yusuke Yonamine, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Keisuke Goda

  The advent of image-activated cell sorting and imaging-based cell picking has advanced our knowledge and exploitation of biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure of the molecular landscape in the cell, which has critical limitations. Here we demonstrate Raman image-activated cell sorting by directly probing chemically specific intracellular molecular vibrations via ultrafast multicolor stimulated Raman scattering (SRS) microscopy for cellular phenotyping. Specifically, the technology enables real-time SRS-image-based sorting of single live cells with a throughput of up to ~100 events per second without the need for fluorescent labeling. To show the broad utility of the technology, we show its applicability to diverse cell types and sizes. The technology is highly versatile and holds promise for numerous applications that are previously difficult or undesirable with fluorescence-based technologies.

  Springer Science and Business Media LLC, Dec. 2020, Nature Communications, 11(1) (1), 3452, English

  [Refereed]

  Scientific journal


• Consolidated bioprocessing of corn cob-derived hemicellulose: engineered industrial Saccharomyces cerevisiae as efficient whole cell biocatalysts

  Joana T. Cunha, Aloia Romaní, Kentaro Inokuma, Björn Johansson, Tomohisa Hasunuma, Akihiko Kondo, Lucília Domingues

  Consolidated bioprocessing, which combines saccharolytic and fermentative abilities in a single microorganism, is receiving increased attention to decrease environmental and economic costs in lignocellulosic biorefineries. Nevertheless, the economic viability of lignocellulosic ethanol is also dependent of an efficient utilization of the hemicellulosic fraction, which is mainly composed of xylose and may comprise up to 40 % of the total biomass. This major bottleneck is mainly due to the necessity of chemical/enzymatic treatments to hydrolyze hemicellulose into fermentable sugars and to the fact that xylose is not readily consumed by Saccharomyces cerevisiae – the most used organism for large-scale ethanol production. In this work, industrial S. cerevisiae strains, presenting robust traits such as thermotolerance and improved resistance to inhibitors, were evaluated as hosts for the cell-surface display of hemicellulolytic enzymes and optimized xylose assimilation, aiming at the development of whole-cell biocatalysts for consolidated bioprocessing of corn cob-derived hemicellulose. These modifications allowed the direct production of ethanol from non-detoxified hemicellulosic liquor obtained by hydrothermal pretreatment of corn cob, reaching an ethanol titer of 11.1 g/L corresponding to a yield of 0.328 gram per gram of potential xylose and glucose, without the need for external hydrolytic catalysts. Also, consolidated bioprocessing of pretreated corn cob was found to be more efficient for hemicellulosic ethanol production than simultaneous saccharification and fermentation with addition of commercial hemicellulases. These results show the potential of industrial S. cerevisiae strains for the design of whole-cell biocatalysts and paves the way for the development of more efficient consolidated bioprocesses for lignocellulosic biomass valorization, further decreasing environmental and economic costs.

  Cold Spring Harbor Laboratory, Jul. 2020, Biotechnology for Biofuels, 13(1) (1), 1 - 15, English

  [Refereed]

  Scientific journal


• Optimizing real swine wastewater treatment efficiency and carbohydrate productivity of newly microalga Chlamydomonas sp. QWY37 used for cell-displayed bioethanol production

  Wenying Qu, Pau Loke Show, Tomohisa Hasunuma, Shih-Hsin Ho

  Elsevier BV, Jun. 2020, Bioresource Technology, 305, 123072 - 123072, English

  [Refereed]

  Scientific journal


• Sequentially addressable dielectrophoretic array for high-throughput sorting of large-volume biological compartments

  A. Isozaki, Y. Nakagawa, M. H. Loo, Y. Shibata, N. Tanaka, D. L. Setyaningrum, J.-W. Park, Y. Shirasaki, H. Mikami, D. Huang, H. Tsoi, C. T. Riche, T. Ota, H. Miwa, Y. Kanda, T. Ito, K. Yamada, O. Iwata, K. Suzuki, S. Ohnuki, Y. Ohya, Y. Kato, T. Hasunuma, S. Matsusaka, M. Yamagishi, M. Yazawa, S. Uemura, K. Nagasawa, H. Watarai, D. Di Carlo, K. Goda

  Droplet microfluidics has become a powerful tool in precision medicine, green biotechnology, and cell therapy for single-cell analysis and selection by virtue of its ability to effectively confine cells. However, there remains a fundamental trade-off between droplet volume and sorting throughput, limiting the advantages of droplet microfluidics to small droplets (<10 pl) that are incompatible with long-term maintenance and growth of most cells. We present a sequentially addressable dielectrophoretic array (SADA) sorter to overcome this problem. The SADA sorter uses an on-chip array of electrodes activated and deactivated in a sequence synchronized to the speed and position of a passing target droplet to deliver an accumulated dielectrophoretic force and gently pull it in the direction of sorting in a high-speed flow. We use it to demonstrate large-droplet sorting with ~20-fold higher throughputs than conventional techniques and apply it to long-term single-cell analysis of Saccharomyces cerevisiae based on their growth rate.

  American Association for the Advancement of Science (AAAS), May 2020, Science Advances, 6(22) (22), eaba6712 - eaba6712, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Malic Enzyme Facilitates d-Lactate Production through Increased Pyruvate Supply during Anoxic Dark Fermentation in Synechocystis sp. PCC 6803.

  Ryota Hidese, Mami Matsuda, Takashi Osanai, Tomohisa Hasunuma, Akihiko Kondo

  d-Lactate is one of the most valuable compounds for manufacturing biobased polymers. Here, we have investigated the significance of endogenous malate dehydrogenase (decarboxylating) (malic enzyme, ME), which catalyzes the oxidative decarboxylation of malate to pyruvate, in d-lactate biosynthesis in the cyanobacterium Synechocystis sp. PCC6803. d-Lactate levels were increased by 2-fold in ME-overexpressing strains, while levels in ME-deficient strains were almost equivalent to those in the host strain. Dynamic metabolomics revealed that overexpression of ME led to increased turnover rates in malate and pyruvate metabolism; in contrast, deletion of ME resulted in increased pool sizes of glycolytic intermediates, probably due to sequential feedback inhibition, initially triggered by malate accumulation. Finally, both the loss of the acetate kinase gene and overexpression of endogenous d-lactate dehydrogenase, concurrent with ME overexpression, resulted in the highest production of d-lactate (26.6 g/L) with an initial cell concentration of 75 g-DCW/L after 72 h fermentation.

  Feb. 2020, ACS synthetic biology, 9(2) (2), 260 - 268, English, International magazine

  [Refereed]

  Link to Kobe Univ. Repository (Kernel)


• Novel strategy for anchorage position control of GPI-attached proteins in the yeast cell wall using different GPI-anchoring domains

  Kentaro Inokuma, Hiroki Kurono, Riaan den, Haan, Willem Heber van Zyl, Tomohisa Hasunuma, Akihiko Kondo

  Jan. 2020, Metabolic Engineering, 57, 110 - 117, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Dynamic metabolomics for engineering biology: Accelerating learning cycles for bioproduction

  Christopher J. Vavricka, Tomohisa Hasunuma, Akihiko Kondo

  Jan. 2020, Trends in Biotechnology, 38(1) (1), 68 - 82, English

  [Refereed]

  Scientific journal


• Production of 1,2,4-butanetriol from xylose by Saccharomyces cerevisiae through Fe metabolic engineering

  Takahiro Bamba, Takahiro Yukawa, Gregory Guirimand, Kentaro Inokuma, Kengo Sasaki, Tomohisa Hasunuma, Akihiko Kondo

  Dec. 2019, Metabolic Engineering, 56, 17 - 27, English

  [Refereed]

  Scientific journal


• Mechanism-based tuning of insect 3,4-dihydroxyphenylacetaldehyde synthase for synthetic bioproduction of benzylisoquinoline alkaloids

  Christopher J. Vavricka, Takanobu Yoshida, Yuki Kuriya, Shunsuke Takahashi, Teppei Ogawa, Fumie Ono, Kazuko Agari, Hiromasa Kiyota, Jianyong Li, Jun Ishii, Kenji Tsuge, Hiromichi Minami, Michihiro Araki, Tomohisa Hasunuma, Akihiko Kondo

  Dec. 2019, Nature Communications, 10(1) (1)

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Short-term temporal metabolic behavior in halophilic cyanobacterium Synechococcus sp. strain PCC 7002 after salt shock

  Shimpei Aikawa, Atsumi Nishida, Tomohisa Hasunuma, Jo-Shu Chang, Akihiko Kondo

  Dec. 2019, Metabolites, 9(12) (12), 297, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Fermentation of pigment-extracted microalgal residue using yeast cell-surface display: direct high-density ethanol production with competitive life cycle impacts

  Xiaochen Huang, Shunwen Bai, Zhuo Liu, Tomohisa Hasunuma, Akihiko Kondo, Shih-Hsin Ho

  Nov. 2019, Green Chemistry, 22(1) (1), 153 - 162, English

  [Refereed]

  Scientific journal


• A novel process for the mixotrophic production of lutein with Chlorella sorokiniana MB-1-M12 using aquaculture wastewater

  Jih-Heng Chen, Yuichi Kato, Mami Matsuda, Chun-Yen Chen, Dillirani Nagarajan, Tomohisa Hasunuma, Akihiko Kondo, Cheng-Di Dong, Duu-Jong Lee, Jo-Shu Chang

  In this study, microalgal cultivation was applied as a feasible strategy for treating shrimp culture wastewater (SCW) from a shrimp farm in southern Tainan. Chlorella sorokiniana MB-1-M12 was first grown on BG-11 medium with 0.5% salinity, obtaining a biomass concentration and productivity of 4.35 g/L and 1.56 g/L/d, respectively. When 80% of BG-11 nutrients were added to 75% strength SCW, lutein content and productivity increased to 5.19 mg/g and 5.55 mg/L/d, respectively. A novel operation strategy involving periodic exchange of freshwater and SCW was designed for semi-continuous cultivation of MB-1-M12 strain for optimal biomass and lutein production. The average biomass concentration, productivity, lutein content, and productivity were 3.5 g/L, 1.3 g/L/d, 3.89 mg/g and 5.0 mg/L/d, respectively. Although microalgae have been considered as an alternative natural source of lutein, this work is among the earliest reports describing lutein production from microalgae cultivated with wastewater via a circular economy concept.

  Oct. 2019, Bioresource Technology, 290, 121786 - 121786, English, International magazine

  [Refereed]

  Scientific journal


• Single-Stage Astaxanthin Production Enhances the Nonmevalonate Pathway and Photosynthetic Central Metabolism in Synechococcus sp. PCC 7002

  Tomohisa Hasunuma, Ayako Takaki, Mami Matsuda, Yuichi Kato, Christopher J. Vavricka, Akihiko Kondo

  Oct. 2019, ACS Synthetic Biology, 8, 2701 - 2709, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Combined cell surface display of β-D-glucosidase (BGL), maltose transporter (MAL11) and overexpression of cytosolic xylose reductase (XR) in Saccharomyces cerevisiae enhance cellobiose/xylose co-utilization for xylitol bio-production from lignocellulosic

  Gregory G, Y. Guirimand, Takahiro Bamba, Mami Matsuda, Kentaro Inokuma, Kenta Morita, Yuki Kitada, Yuma Kobayashi, Takahiro Yukawa, Kengo Sasaki, Chiaki Ogino, Tomohisa Hasunuma, Akihiko Kondo

  Xylitol is a highly valuable commodity chemical used extensively in the food and pharmaceutical industries. The production of xylitol from d-xylose involves a costly and polluting catalytic hydrogenation process. Biotechnological production from lignocellulosic biomass by micro-organisms like yeasts is a promising option. In this study, xylitol is produced from lignocellulosic biomass by a recombinant strain of Saccharomyces cerevisiae (S. cerevisiae) (YPH499-SsXR-AaBGL) expressing cytosolic xylose reductase (Scheffersomyces stipitis xylose reductase [SsXR]), along with a β-d-glucosidase (Aspergillus aculeatus β-glucosidase 1 [AaBGL]) displayed on the cell surface. The simultaneous cofermentation of cellobiose/xylose by this strain leads to an ≈2.5-fold increase in Yxylitol/xylose (=0.54) compared to the use of a glucose/xylose mixture as a substrate. Further improvement in the xylose uptake by the cell is achieved by a broad evaluation of several homologous and heterologous transporters. Homologous maltose transporter (ScMAL11) shows the best performance in xylose transport and is used to generate the strain YPH499-XR-ScMAL11-BGL with a significantly improved xylitol production capacity from cellobiose/xylose coutilization. This report constitutes a promising proof of concept to further scale up the biorefinery industrial production of xylitol from lignocellulose by combining cell surface and metabolic engineering in S. cerevisiae.

  Sep. 2019, Biotechnology Journal, 14(9) (9), e1800704, English, International magazine

  [Refereed]

  Scientific journal


• Day/night separation of oxygenic energy metabolism and nuclear DNA replication in the unicellular red alga Cyanidioschyzon merolae

  Shin-ya Miyagishima, Atsuko Era, Tomohisa Hasunuma, Mami Matsuda, Shunsuke Hirooka, Nobuko Sumiya, Akihiko Kondo, Takayuki Fujiwara

  Jul. 2019, mBio, 10(4) (4), e00833-19, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Author Correction: Mechanism-based tuning of insect 3,4-dihydroxyphenylacetaldehyde synthase for synthetic bioproduction of benzylisoquinoline alkaloids.

  Christopher J Vavricka, Takanobu Yoshida, Yuki Kuriya, Shunsuke Takahashi, Teppei Ogawa, Fumie Ono, Kazuko Agari, Hiromasa Kiyota, Jianyong Li, Jun Ishii, Kenji Tsuge, Hiromichi Minami, Michihiro Araki, Tomohisa Hasunuma, Akihiko Kondo

  In the original version of this Article, the abbreviation of 3,4-dihydroxyphenylacetaldehyde synthase presented in the first paragraph of the Discussion section was given incorrectly as DYPAA. The correct abbreviation for this enzyme is DHPAAS. This error has been corrected in both the PDF and HTML versions of the Article.

  May 2019, Nature communications, 10(1) (1), 2336 - 2336, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Cell-surface display technology and metabolic engineering of Saccharomyces cerevisiae for enhancing xylitol production from woody biomass

  Gregory Guirimand, Kentaro Inokuma, Takahiro Bamba, Mami Matsuda, Kenta Morita, Kengo Sasaki, Chiaki Ogino, Jean-Guy Berrin, Tomohisa Hasunuma, Akihiko Kondo

  May 2019, Green Chemistry, 21, 1795 - 1808, English

  [Refereed]

  Scientific journal


• Enhancing lutein production with mixotrophic cultivation of Chlorella sorokiniana MB-1-M12 using different bioprocess operation strategies

  Jih-Heng Chen, Chun-Yen Chen, Tomohisa Hasunuma, Akihiko Kondo, Chien-Hsiang Chang, I-Son Ng, Jo-Shu Chang

  Apr. 2019, Bioresource Technology, 278, 17 - 25, English

  [Refereed]

  Scientific journal


• Increased flux in acetyl-CoA synthetic pathway and TCA cycle of Kluyveromyces marxianus under respiratory conditions

  SAKIHAMA Yuri, HIDESE Ryota, HASUNUMA Tomohisa, KONDO Akihiko

  Yeasts are extremely useful, not only for fermentation but also for a wide spectrum of fuel and chemical productions. We analyzed the overall metabolic turnover and transcript dynamics in glycolysis and the TCA cycle, revealing the difference in adaptive pyruvate metabolic response between a Crabtree-negative species, Kluyveromyces marxianus, and a Crabtree-positive species, Saccharomyces cerevisiae, during aerobic growth. Pyruvate metabolism was inclined toward ethanol production under aerobic conditions in S. cerevisiae, while increased transcript abundances of the genes involved in ethanol metabolism and those encoding pyruvate dehydrogenase were seen in K. marxianus, indicating the augmentation of acetyl-CoA synthesis. Furthermore, different metabolic turnover in the TCA cycle was observed in the two species: malate and fumarate production in S. cerevisiae was higher than in K. marxianus, irrespective of aeration; however, fluxes of both the reductive and oxidative TCA cycles were enhanced in K. marxianus by aeration, implying both the cycles contribute to efficient electron flux without producing ethanol. Additionally, decreased hexokinase activity under aerobic conditions is expected to be important for maintenance of suitable carbon flux. These findings demonstrate differences in the key metabolic trait of yeasts employing respiration or fermentation, and provide important insight into the metabolic engineering of yeasts.

  Mar. 2019, Scientific Reports, 9(1) (1), 5319 - 5319, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Sustainable production of glutathione from lignocellulose-derived sugars using engineered Saccharomyces cerevisiae

  KOBAYASHI Jyumpei, SASAKI Daisuke, BAMBA Takahiro, HASUNUMA Tomohisa, KONDO Akihiko

  Feb. 2019, Applied Microbiology and Biotechnology, 103(3) (3), 1243 - 1254, English

  [Refereed]

  Scientific journal


• Light/dark cycling causes delayed lipid accumulation and increased photoperiod-based biomass yield by altering metabolic flux in oleaginous Chlamydomonas sp.

  Yuichi KATO, Yusuke FUJIHARA, Christopher J. VAVRICKA, Jo-Shu CHANG, Tomohisa HASUNUMA, Akihiko KONDO

  BACKGROUND: Light/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on cellular lipid production has not been clearly established. The general aim of this study was to determine the influence of light/dark cycling on microalgal biomass production and lipid accumulation. To achieve this goal, specific causative mechanisms were investigated using a metabolomics approach. Laboratory scale photoautotrophic cultivations of the oleaginous green microalga Chlamydomonas sp. JSC4 were performed under continuous light (LL) and light/dark (LD) conditions. RESULTS: Lipid accumulation and carbohydrate degradation were delayed under the LD condition compared with that under the LL condition. Metabolomic analysis revealed accumulation of phosphoenolpyruvate and decrease of glycerol 3-phosphate under the LD condition, suggesting that the imbalance of these metabolites is a source of delayed lipid accumulation. When accounting for light dosage, biomass yield under the LD condition was significantly higher than that under the LL condition. Dynamic metabolic profiling showed higher levels of lipid/carbohydrate anabolism (including production of 3-phosphoglycerate, fructose 6-phosphate, glucose 6-phosphate, phosphoenolpyruvate and acetyl-CoA) from CO2 under the LD condition, indicating higher CO2 fixation than that of the LL condition. CONCLUSIONS: Photoperiods define lipid accumulation and biomass production, and light/dark cycling was determined as a critical obstacle for lipid production in JSC4. Conversions of phosphoenolpyruvate to pyruvate and 3-phosphoglycerate to glycerol 3-phosphate are the candidate rate-limiting steps responsible for delayed lipid accumulation. The accumulation of substrates including ribulose 5-phosphate could be explained by the close relationship of increased biomass yield with enhanced CO2 fixation. The present study investigated the influence of light/dark cycling on lipid production by direct comparison with continuous illumination for the first time, and revealed underlying metabolic mechanisms and candidate metabolic rate-limiting steps during light/dark cycling. These findings suggest promising targets to metabolically engineer improved lipid production.

  Feb. 2019, Biotechnology for Biofuels, 12, 39 - 39, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• 5-Hydroxymethylfurfural production from salt-induced photoautotrophically cultivated Chlorella sorokiniana

  AMOAH Jerome, HASUNUMA Tomohisa, OGINO Chiaki, KONDO Akihiko

  Feb. 2019, Biochemical Engineering Journal, 142, 117 - 123, English

  [Refereed]

  Scientific journal


• Deletion of DNA ligase IV homolog confers higher gene targeting efficiency on homologous recombination in Komagataella phaffii

  Yoichiro Ito, Toru Watanabe, Shimpei Aikawa, Teruyuki Nishi, Tozo Nishiyama, Yasuyuki Nakamura, Tomohisa Hasunuma, Yuji Okubo, Jun Ishii, Akihiko Kondo

  Oxford University Press (OUP), Nov. 2018, FEMS Yeast Research, 18(7) (7), foy074, English

  [Refereed]

  Scientific journal


• Widespread effect of N-acetyl-D-glucosamine assimilation on the metabolisms of amino acids, purines, and pyrimidines in Scheffersomyces stipitis

  INOKUMA Kentaro, MATSUDA Mami, SASAKI Daisuke, HASUNUMA Tomohisa, KONDO Akihiko

  Sep. 2018, Microbial Cell Factories, 17, 153, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Estimating the protein burden limit of yeast cells by measuring the expression limits of glycolytic proteins.

  Yuichi Eguchi, Koji Makanae, Tomohisa Hasunuma, Yuko Ishibashi, Keiji Kito, Hisao Moriya

  The ultimate overexpression of a protein could cause growth defects, which are known as the protein burden. However, the expression limit at which the protein-burden effect is triggered is still unclear. To estimate this limit, we systematically measured the overexpression limits of glycolytic proteins in Saccharomyces cerevisiae. The limits of some glycolytic proteins were up to 15% of the total cellular protein. These limits were independent of the proteins' catalytic activities, a finding that was supported by an in silico analysis. Some proteins had low expression limits that were explained by their localization and metabolic perturbations. The codon usage should be highly optimized to trigger the protein-burden effect, even under strong transcriptional induction. The S-S-bond-connected aggregation mediated by the cysteine residues of a protein might affect its expression limit. Theoretically, only non-harmful proteins could be expressed up to the protein-burden limit. Therefore, we established a framework to distinguish proteins that are harmful and non-harmful upon overexpression.

  Aug. 2018, eLife, 7, e34595, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Temperature enhanced succinate production concurrent with increased central metabolism turnover in the cyanobacterium Synechocystis sp. PCC 6803

  Tomohisa Hasunuma, Mami Matsuda, Yuichi Kato, Christopher John Vavricka, Akihiko Kondo

  Academic Press Inc., Jul. 2018, Metabolic Engineering, 48, 109 - 120, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A stable, autonomously replicating plasmid vector containing Pichia pastoris centromeric DNA

  Yasuyuki Nakamura, Teruyuki Nishi, Risa Noguchi, Yoichiro Ito, Toru Watanabe, Tozo Nishiyama, Sinpei Aikawa, Tomohisa Hasunuma, Jun Ishii, Yuji Okubo, Akihiko Kondo

  The methylotrophic yeast Pichia pastoris is widely used to produce recombinant proteins, taking advantage of this species' high-density cell growth and strong ability to secrete proteins. Circular plasmids containing the P. pastoris-specific autonomously replicating sequence (PARS1) permit transformation of P. pastoris with higher efficiency than obtained following chromosomal integration by linearized DNA. Unfortunately, however, existing autonomously replicating plasmids are known to be inherently unstable. In this study, we used transcriptome sequencing (RNA-seq) data and genome sequence information to independently identify, on each of the four chromosomes, centromeric DNA sequences consisting of long inverted repeat sequences. By examining the chromosome 2 centromeric DNA sequence (Cen2) in detail, we demonstrate that an ∼111-bp region located at one end of the putative centromeric sequence had autonomous replication activity. In addition, the full-length Cen2 sequence, which contains two long inverted repeat sequences and a nonrepetitive central core region, is needed for the accurate replication and distribution of plasmids in P. pastoris. Thus, we constructed a new, stable, autonomously replicating plasmid vector that harbors the entire Cen2 sequence; this episome facilitates genetic manipulation in P. pastoris, providing high transformation efficiency and plasmid stability. IMPORTANCE Secretory production of recombinant proteins is the most important application of the methylotrophic yeast Pichia pastoris, a species that permits mass production of heterologous proteins. To date, the genetic engineering of P. pastoris has relied largely on integrative vectors due to the lack of user-friendly tools. Autonomously replicating Pichia plasmids are expected to facilitate genetic manipulation; however, the existing systems, which use autonomously replicating sequences (ARSs) such as the P. pastoris-specific ARS (PARS1), are known to be inherently unstable for plasmid replication and distribution. Recently, the centromeric DNA sequences of P. pastoris were identified in back-to-back studies published by several groups; therefore, a new episomal plasmid vector with centromere DNA as a tool for genetic manipulation of P. pastoris is ready to be developed.

  American Society for Microbiology, Jul. 2018, Applied and Environmental Microbiology, 84(15) (15), e02882 - 17, English

  [Refereed]

  Scientific journal


• Metabolome analysis-based design and engineering of a metabolic pathway in Corynebacterium glutamicum to match rates of simultaneous utilization of d-glucose and l-arabinose

  Hideo Kawaguchi, Kumiko Yoshihara, Kiyotaka Y. Hara, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  BioMed Central Ltd., May 2018, Microbial Cell Factories, 17(1) (1), 76, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Mathematical model for small size time series data of bacterial secondary metabolic pathways

  TOMINAGA D, KAWAGUCHI Hideo, HORI Yoshimi, HASUNUMA Tomohisa, OGINO Chiaki, ABURATANI S

  Measuring the concentrations of metabolites and estimating the reaction rates of each reaction step consisting of metabolic pathways are significant for an improvement in microorganisms used in maximizing the production of materials. Although the reaction pathway must be identified for such an improvement, doing so is not easy. Numerous reaction steps have been reported; however, the actual reaction steps activated vary or change according to the conditions. Furthermore, to build mathematical models for a dynamical analysis, the reaction mechanisms and parameter values must be known; however, to date, sufficient information has yet to be published for many cases. In addition, experimental observations are expensive. A new mathematical approach that is applicable to small sample data, and that requires no detailed reaction information, is strongly needed. S-system is one such model that can use smaller samples than other ordinary differential equation models. We propose a simplified S-system to apply minimal quantities of samples for a dynamic analysis of the metabolic pathways. We applied the model to the phenyl lactate production pathway of Escherichia coli. The model obtained suggests that actually activated reaction steps and feedback are inhibitions within the pathway.

  May 2018, Bioinformatics and Biology Insights, 12, 1 - 7, English, International magazine

  [Refereed]

  Scientific journal


• Genetic and physiological basis for antibody production by Kluyveromyces marxianus

  NAMBU-NISHIDA Yumiko, NISHIDA Keiji, HASUNUMA Tomohisa, KONDO Akihiko

  Apr. 2018, AMB Express, 8, 56, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Enhanced cell-surface display of a heterologous protein using SED1 anchoring system in SED1-disrupted Saccharomyces cerevisiae strain

  Takahiro Bamba, Kentaro Inokuma, Tomohisa Hasunuma, Akihiko Kondo

  Elsevier B.V., Mar. 2018, Journal of Bioscience and Bioengineering, 125(3) (3), 346 - 352, English

  [Refereed]

  Scientific journal


• Direct and highly productive conversion of cyanobacteria Arthrospira platensis to ethanol with CaCl2 addition

  Shimpei Aikawa, Kentaro Inokuma, Satoshi Wakai, Kengo Sasaki, Chiaki Ogino, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo

  BioMed Central Ltd., Feb. 2018, Biotechnology for Biofuels, 11(1) (1), 50, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Selection of yeast Saccharomyces cerevisiae promoters available for xylose cultivation and fermentation

  Yumiko Nambu-Nishida, Yuri Sakihama, Jun Ishii, Tomohisa Hasunuma, Akihiko Kondo

  Elsevier B.V., Jan. 2018, Journal of Bioscience and Bioengineering, 125(1) (1), 76 - 86, English

  [Refereed]

  Scientific journal


• Improvement of Xylose Fermentation Ability under Heat and Acid Co-Stress in Saccharomyces cerevisiae Using Genome Shuffling Technique

  Kentaro Inokuma, Ryo Iwamoto, Takahiro Bamba, Tomohisa Hasunuma, Akihiko Kondo

  Dec. 2017, Frontiers in Bioengineering and Biotechnology, 5, 81, English

  [Refereed]

  Scientific journal


• Evolutionary engineering of salt-resistant Chlamydomonas sp strains reveals salinity stress-activated starch-to-lipid biosynthesis switching

  Yuichi Kato, Shih-Hsin Ho, Christopher J. Vavricka, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo

  Dec. 2017, BIORESOURCE TECHNOLOGY, 245(part B) (part B), 1484 - 1490, English

  [Refereed]

  Scientific journal


• Conversion of Chlamydomonas sp JSC4 lipids to biodiesel using Fusarium heterosporum lipase-expressing Aspergillus oryzae whole-cell as biocatalyst

  Jerome Amoah, Shih-Hsin Ho, Shinji Hama, Ayumi Yoshida, Akihito Nakanishi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Dec. 2017, ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 28, 16 - 23, English

  [Refereed]

  Scientific journal


• Quantitative metabolomics of a xylose-utilizing Saccharomyces cerevisiae strain expressing the Bacteroides thetaiotaomicron xylose isomerase on glucose and xylose

  M. J. Mert, S. H. Rose, D. C. la Grange, T. Bamba, T. Hasunuma, A. Kondo, W. H. van Zyl

  Oct. 2017, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 44(10) (10), 1459 - 1470, English

  [Refereed]

  Scientific journal


• Synthesis of Sulfo-Sialic Acid Analogues: Potent Neuraminidase Inhibitors in Regards to Anomeric Functionality

  Christopher J. Vavricka, Chiaki Muto, Tomohisa Hasunuma, Yoshinobu Kimura, Michihiro Araki, Yan Wu, George F. Gao, Hiroshi Ohrui, Minoru Izumi, Hiromasa Kiyota

  Aug. 2017, SCIENTIFIC REPORTS, 7(1) (1), 8239, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Development of a comprehensive set of tools for genome engineering in a cold- and thermo-tolerant Kluyveromyces marxianus yeast strain

  Yumiko Nambu-Nishida, Keiji Nishida, Tomohisa Hasunuma, Akihiko Kondo

  Aug. 2017, SCIENTIFIC REPORTS, 7(1) (1), 8993, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Improvement of ethanol production from crystalline cellulose via optimizing cellulase ratios in cellulolytic Saccharomyces cerevisiae

  Zhuo Liu, Kentaro Inokuma, Shih-Hsin Ho, Riaan den Haan, Willem H. van Zyl, Tomohisa Hasunuma, Akihiko Kondo

  Jun. 2017, BIOTECHNOLOGY AND BIOENGINEERING, 114(6) (6), 1201 - 1207, English

  [Refereed]

  Scientific journal


• Dynamic metabolic profiling together with transcription analysis reveals salinity-induced starch-to-lipid biosynthesis in alga Chlamydomonas sp JSC4

  Shih-Hsin Ho, Akihito Nakanishi, Yuichi Kato, Hiroaki Yamasaki, Jo-Shu Chang, Naomi Misawa, Yuu Hirose, Jun Minagawa, Tomohisa Hasunuma, Akihiko Kondo

  Apr. 2017, SCIENTIFIC REPORTS, 7(7) (7), 45471, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• High-Speed Scanning for the Quantitative Evaluation of Glycogen Concentration in Bioethanol Feedstock Synechocystis sp PCC6803 Using a Near-Infrared Hyperspectral Imaging System with a New Near-Infrared Spectral Camera

  Mika Ishigaki, Akihito Nakanishi, Tomohisa Hasunuma, Akihiko Kondo, Tetsu Morishima, Toshiaki Okuno, Yukihiro Ozaki

  Mar. 2017, APPLIED SPECTROSCOPY, 71(3) (3), 463 - 471, English

  [Refereed]

  Scientific journal


• Simultaneous conversion of free fatty acids and triglycerides to biodiesel by immobilized Aspergillus oryzae expressing Fusarium heterosporum lipase

  Jerome Amoah, Emmanuel Quayson, Shinji Hama, Ayumi Yoshida, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Mar. 2017, BIOTECHNOLOGY JOURNAL, 12(3) (3), 1600400, English

  [Refereed]

  Scientific journal


• Mapping of endoglucanases displayed on yeast cell surface using atomic force microscopy

  Musashi Takenaka, Takuya Kobayashi, Kentaro Inokuma, Tomohisa Hasunuma, Tatsuo Maruyama, Chiaki Ogino, Akihiko Kondo

  Mar. 2017, COLLOIDS AND SURFACES B-BIOINTERFACES, 151, 134 - 142, English

  [Refereed]

  Scientific journal


• Enzymatic improvement of mitochondrial thiol oxidase Erv1 for oxidized glutathione fermentation by Saccharomyces cerevisiae

  Jyumpei Kobayashi, Daisuke Sasaki, Kiyotaka Y. Hara, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2017, MICROBIAL CELL FACTORIES, 16, 44, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• A Systematic Approach to Timeseries Metabolite Profiling and RNA-seq Analysis of Chinese Hamster Ovary Cell Culture

  Han-Hsiu Hsu, Michihiro Araki, Masao Mochizuki, Yoshimi Hori, Masahiro Murata, Prihardi Kahar, Takanobu Yoshida, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2017, SCIENTIFIC REPORTS, 7, 43518, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Application of THz Vibrational Spectroscopy to Molecular Characterization and the Theoretical Fundamentals: An Illustration Using Saccharide Molecules

  Feng Zhang, Houng-Wei Wang, Keisuke Tominaga, Michitoshi Hayashi, Tomohisa Hasunuma, Akihiko Kondo

  Feb. 2017, CHEMISTRY-AN ASIAN JOURNAL, 12(3) (3), 324 - 331, English

  [Refereed]

  Scientific journal


• Application of LC-MS/MS analysis for time-lapse amino acid metabolomics in CHO cell culture.

  Hsu H, Hasunuma T, Araki M, Yoshida T, Hori Y, Murata M, Kondo A

  Feb. 2017, Shimadzu Journal, 5(1) (1), 17 - 21, English

  [Refereed]

  Scientific journal


• Whole cell biocatalysts using enzymes displayed on yeast cell surface

  Kentaro Inokuma, Tomohisa Hasunuma, Akihiko Kondo

  wiley, Jan. 2017, Emerging Areas in Bioengineering, 81 - 92, English

  In book


• O-19 Development of the novel yeast cell-surface display cassette for consolidated bioethanol production from biomass resources

  INOKUMA Kentaro, HASUNUMA Tomohisa, KONDO Akihiko

  The recombinant yeast strains displaying the heterologous cellulolytic enzymes on the cell surface using the glycosylphosphatidylinositol (GPI) anchoring system are considered promising biocatalysts for the consolidated bioethanol production from lignocellulosic biomass. In this study, we constructed novel gene cassettes for the efficient cellulase display on yeast cell surface. We revealed that simultaneous utilization of the GPI anchoring region derived from Saccharomyces cerevisiae SED1 and its original promoter in a gene cassette enabled highly-efficient enzyme integration into the cell wall. The β-glucosidase and endoglucanase activities of recombinant yeast cells transduced with the novel gene cassette were 8.4- and 106-fold higher than those of conventional strains. The novel gene cassette also improved cell-surface hemicellulase activity. These results suggest that the novel gene cassette has the wide applicability for efficient cell-surface display of heterologous enzymes and that recombinant yeast cells displaying enzymes using these cassettes are promising biocatalysts for the efficient ethanol production from biomass resources.

  The Japan Institute of Energy, 2017, Proceedings of the Conference on Biomass Science, 12, 37 - 38, Japanese


• Erratum for Inokuma et al., Complete genome sequence of Kluyveromyces marxianus NBRC1777, a nonconventional thermotolerant yeast [3, 2, e00389-15 (1-1) (2015)]

  Kentaro Inokuma, Jun Ishii, Kiyotaka Y. Hara, Masao Mochizuki, Tomohisa Hasunuma, Akihiko Kondo

  American Society for Microbiology, 2017, Genome Announcements, 5(5) (5), English

  [Refereed]

  Scientific journal


• A RuBisCO-mediated carbon metabolic pathway in methanogenic archaea

  Takunari Kono, Sandhya Mehrotra, Chikako Endo, Natsuko Kizu, Mami Matusda, Hiroyuki Kimura, Eiichi Mizohata, Tsuyoshi Inoue, Tomohisa Hasunuma, Akiho Yokota, Hiroyoshi Matsumura, Hiroki Ashida

  Jan. 2017, NATURE COMMUNICATIONS, 8, 14007, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Succinate and Lactate Production from Euglena gracilis during Dark, Anaerobic Conditions

  Yuko Tomita, Kazumasa Yoshioka, Hiroko Iijima, Ayaka Nakashima, Osamu Iwata, Kengo Suzuki, Tomohisa Hasunuma, Akihiko Kondo, Masami Yokota Hirai, Takashi Osanai

  Dec. 2016, FRONTIERS IN MICROBIOLOGY, 7, 2050, English

  [Refereed]

  Scientific journal


• Bioprocessing of bio-based chemicals produced from lignocellulosic feedstocks

  Hideo Kawaguchi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Dec. 2016, CURRENT OPINION IN BIOTECHNOLOGY, 42, 30 - 39, English

  [Refereed]

  Scientific journal


• Production of fuels and chemicals from biomass by integrated bioprocesses

  Tomohisa Hasunuma, Akihiko Kondo

  Wiley-VCH Verlag, Nov. 2016, Industrial Biotechnology: Products and Processes, 161 - 185, English

  [Refereed]

  In book


• Primary Metabolism during Biosynthesis of Secondary Wall Polymers of Protoxylem Vessel Elements

  Misato Ohtani, Keiko Morisaki, Yuji Sawada, Ryosuke Sano, Abigail Loren Tung Uy, Atsushi Yamamoto, Tetsuya Kurata, Yoshimi Nakano, Shiro Suzuki, Mami Matsuda, Tomohisa Hasunuma, Masami Yokota Hirai, Taku Demura

  Nov. 2016, PLANT PHYSIOLOGY, 172(3) (3), 1612 - 1624, English

  [Refereed]

  Scientific journal


• Moderate Heat Stress Stimulates Repair of Photosystem II During Photoinhibition in Synechocystis sp PCC 6803

  Mamoru Ueno, Penporn Sae-Tang, Yuri Kusama, Yukako Hihara, Mami Matsuda, Tomohisa Hasunuma, Yoshitaka Nishiyama

  Nov. 2016, PLANT AND CELL PHYSIOLOGY, 57(11) (11), 2417 - 2426, English

  [Refereed]

  Scientific journal


• Enhanced Cell-Surface Display and Secretory Production of Cellulolytic Enzymes With Saccharomyces cerevisiae Sed1 Signal Peptide

  Kentaro Inokuma, Takahiro Bamba, Jun Ishii, Yoichiro Ito, Tomohisa Hasunuma, Akihiko Kondo

  Nov. 2016, BIOTECHNOLOGY AND BIOENGINEERING, 113(11) (11), 2358 - 2366, English

  [Refereed]

  Scientific journal


• Ethanol production from N-acetyl-D-glucosamine by Scheffersomyces stipitis strains

  Kentaro Inokuma, Tomohisa Hasunuma, Akihiko Kondo

  Oct. 2016, AMB EXPRESS, 6, 83, English

  [Refereed]

  Scientific journal


• Recent advances in yeast cell-surface display technologies for waste biorefineries

  Zhuo Liu, Shih-Hsin Ho, Tomohisa Hasunuma, Jo-Shu Chang, Nan-Qi Ren, Akihiko Kondo

  Sep. 2016, BIORESOURCE TECHNOLOGY, 215, 324 - 333, English

  [Refereed]

  Scientific journal


• Metabolomics-based analysis revealing the alteration of primary carbon metabolism by the genetic manipulation of a hydrogenase HoxH in Synechocystis sp PCC 6803

  Hiroko Iijima, Tomokazu Shirai, Mami Okamoto, Filipe Pinto, Paula Tamagnini, Tomohisa Hasunuma, Akihiko Kondo, Masami Yokota Hirai, Takashi Osanai

  Sep. 2016, ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 18, 305 - 313, English

  [Refereed]

  Scientific journal


• Improving carbohydrate production of Chlorella sorokiniana NIES-2168 through semi-continuous process coupled with mixotrophic cultivation

  Yue Wang, Sheng-Yi Chiu, Shih-Hsin Ho, Zhuo Liu, Tomohisa Hasunuma, Ting-Ting Chang, Kuan-Fu Chang, Jo-Shu Chang, Nan-Qi Ren, Akihiko Kondo

  Aug. 2016, BIOTECHNOLOGY JOURNAL, 11(8) (8), 1072 - 1081, English

  [Refereed]

  Scientific journal


• Lipase cocktail for efficient conversion of oils containing phospholipids to biodiesel

  Jerome Amoah, Shih-Hsin Ho, Shinji Hama, Ayumi Yoshida, Akihito Nakanishi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Jul. 2016, BIORESOURCE TECHNOLOGY, 211, 224 - 230, English

  [Refereed]

  Scientific journal


• Improved sugar-free succinate production by Synechocystis sp. PCC 6803 following identification of the limiting steps in glycogen catabolism.

  Hasunuma T, Matsuda M, Kondo A

  May 2016, Metabolic Engineering Communications, 3, 130 - 141, English

  [Refereed]

  Scientific journal


• Engineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel production

  Zhuo Liu, Shih-Hsin Ho, Kengo Sasaki, Riaan den Haan, Kentaro Inokuma, Chiaki Ogino, Willem H. van Zyl, Tomohisa Hasunuma, Akihiko Kondo

  Apr. 2016, SCIENTIFIC REPORTS, 6, 24550, English

  [Refereed]

  Scientific journal


• Cell surface engineering of Saccharomyces cerevisiae combined with membrane separation technology for xylitol production from rice straw hydrolysate

  Gregory Guirimand, Kengo Sasaki, Kentaro Inokuma, Takahiro Bamba, Tomohisa Hasunuma, Akihiko Kondo

  Apr. 2016, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 100(8) (8), 3477 - 3487, English

  [Refereed]

  Scientific journal


• FudC, a protein primarily responsible for furfural detoxification in Corynebacterium glutamicum

  Yota Tsuge, Motonori Kudou, Hideo Kawaguchi, Jun Ishii, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2016, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 100(6) (6), 2685 - 2692, English

  [Refereed]

  Scientific journal


• Disruption of PHO13 improves ethanol production via the xylose isomerase pathway

  BAMBA Takahiro, HASUNUMA Tomohisa, KONDO Akihiko

  Mar. 2016, AMB Express, 6(1) (1), 4, English

  [Refereed]

  Scientific journal


• O-18 Simultaneous saccharification and fermentation of lignocellulosic biomass for phenyllactate production by recombinant Escherichia coli

  川口,秀夫, 寺村,浩, 中村,聡子, 荻野,千秋, 原,清敬, 蓮沼,誠久, 老沼,研一, 高谷,直樹, 平野,恒, 佐塚,隆志, 北野,英己, 近藤,昭彦

  Sorghum bagasse pretreated with diluted acid, which was predominantly composed of glucan (59%) and xylan (7.2%), was used as a lignocellulosic feedstock to produce D-phenyllactic acid (PhLA) by a recombinant Escherichia coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens. Compared to filter paper hydrolysate, the PhLA yield was reduced by 35% during fermentation with enzymatic hydrolysate of sorghum bagasse as a carbon source, and metabolomics analysis revealed that intracellular levels of erythrose-4-phosphate and phosphoenolpyruvate and NAD(P)H regeneration for PhLA production from glucose markedly reduced. Compared to the separate hydrolysis and fermentation (SHF) with sorghum bagasse hydrolysate, simultaneous saccharification and fermentation (SSF) of sorghum bagasse under glucose limitation conditions yielded 4.8-fold more PhLA with less accumulation of eluted components of p-coumaric acid and aldehydes, which inhibited PhLA fermentation. These results suggest that gradual hydrolysis of sorghum bagasse during SSF reduces the accumulation of both glucose and fermentation inhibitors, collectively leading to increased PhLA yield.

  The Japan Institute of Energy, Jan. 2016, Proceedings of the Conference on Biomass Science, 11, 35 - 36, Japanese

  [Refereed]

  International conference proceedings


• Complete genome sequence of Kluyveromyces marxianus NBRC1777, a nonconventional thermotolerant yeast

  Kentaro Inokuma, Jun Ishii, Kiyotaka Y. Hara, Masao Mochizuki, Tomohisa Hasunuma, Akihiko Kondo

  American Society for Microbiology, 2016, Genome Announcements, 3(2) (2), e00389 - 15, English

  [Refereed]

  Scientific journal


• Inverse metabolic engineering based on transient acclimation of yeast improves acid-containing xylose fermentation and tolerance to formic and acetic acids

  Tomohisa Hasunuma, Takatoshi Sakamoto, Akihiko Kondo

  Jan. 2016, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 100(2) (2), 1027 - 1038, English

  [Refereed]

  Scientific journal


• Converting oils high in phospholipids to biodiesel using immobilized Aspergillus oryzae whole-cell biocatalysts expressing Fusarium heterosporum lipase

  Jerome Amoah, Shih-Hsin Ho, Shinji Hama, Ayumi Yoshida, Akihito Nakanishi, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Jan. 2016, BIOCHEMICAL ENGINEERING JOURNAL, 105, 10 - 15, English

  [Refereed]

  Scientific journal


• Rational design and evolutional fine tuning of Saccharomyces cerevisiae for biomass breakdown

  Tomohisa Hasunuma, Jun Ishii, Akihiko Kondo

  Dec. 2015, CURRENT OPINION IN CHEMICAL BIOLOGY, 29, 1 - 9, English

  [Refereed]

  Scientific journal


• Genetic engineering and metabolite profiling for overproduction of polyhydroxybutyrate in cyanobacteria

  Sayaka Hondo, Masatoshi Takahashi, Takashi Osanai, Mami Matsuda, Tomohisa Hasunuma, Akio Tazuke, Yoichi Nakahira, Shigeru Chohnan, Morifumi Hasegawa, Munehiko Asayama

  Nov. 2015, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 120(5) (5), 510 - 517, English

  [Refereed]

  Scientific journal


• Expression of Cyanobacterial Acyl-ACP Reductase Elevates the Triacylglycerol Level in the Red Alga Cyanidioschyzon merolae

  Nobuko Sumiya, Yasuko Kawase, Jumpei Hayakawa, Mami Matsuda, Mami Nakamura, Atsuko Era, Kan Tanaka, Akihiko Kondo, Tomohisa Hasunuma, Sousuke Imamura, Shin-ya Miyagishima

  Oct. 2015, PLANT AND CELL PHYSIOLOGY, 56(10) (10), 1962 - 1980, English

  [Refereed]

  Scientific journal


• バイオリファイナリーの現状と展望

  HASUNUMA TOMOHISA, ISHII JUN, OGINO CHIAKI, KONDO AKIHIKO

  持続可能な社会へ向かうためには再生可能エネルギーが中心的な役割を果たすことが求められている．そのなかで，バイオマスから液体燃料やバルクケミカルを経済性良く，高効率で生産する技術の開発が期待されている．バイオマスとしては，安定的な供給が可能で，食糧と競合しないリグノセルロース系バイオマスの利活用が望まれている．本稿ではリグノセルロース系バイオマスからのエタノールの製造プロセスについて研究の課題と最新の知見を紹介するとともに，バイオプロセスによるバルクケミカル生産に関する最近の研究例についても紹介する．

  Japan Society for Bioscience, Biotechnology, and Agrochemistry, Oct. 2015, 化学と生物, 53(10) (10), 689 - 695, Japanese

  Scientific journal


• 合成生物工学によるモノづくり微生物のデザインに向けて

  ISHII JUN, ARAKI MICHIHIRO, NAKATSUI MASAHIKO, SAKIHAMA YURI, 柘植 陽太, HASUNUMA TOMOHISA, KONDO AKIHIKO

  Sep. 2015, 生物工学会誌, 93(9) (9), 523 - 526, Japanese

  Scientific journal


• Combined cell-surface display- and secretion-based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae

  Zhuo Liu, Kentaro Inokuma, Shih-Hsin Ho, Riaan den Haan, Tomohisa Hasunuma, Willem H. van Zyl, Akihiko Kondo

  Sep. 2015, BIOTECHNOLOGY FOR BIOFUELS, 8, 162, English

  [Refereed]

  Scientific journal


• Improving polyglucan production in cyanobacteria and microalgae via cultivation design and metabolic engineering

  Shimpei Aikawa, Shih-Hsin Ho, Akihito Nakanishi, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo

  Jun. 2015, BIOTECHNOLOGY JOURNAL, 10(6) (6), 886 - 898, English

  [Refereed]

  Scientific journal


• Precipitate obtained following membrane separation of hydrothermally pretreated rice straw liquid revealed by 2D NMR to have high lignin content

  Kengo Sasaki, Mami Okamoto, Tomokazu Shirai, Yota Tsuge, Hiroshi Teramura, Daisuke Sasaki, Hideo Kawaguchi, Tomohisa Hasunuma, Chiaki Ogino, Fumio Matsuda, Jun Kikuchi, Akihiko Kondo

  Jun. 2015, BIOTECHNOLOGY FOR BIOFUELS, 8, 88, English

  [Refereed]

  Scientific journal


• Mechanical milling and membrane separation for increased ethanol production during simultaneous saccharification and co-fermentation of rice straw by xylose-fermenting Saccharomyces cerevisiae

  Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Hiroshi Teramura, Kentaro Inokuma, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Jun. 2015, BIORESOURCE TECHNOLOGY, 185, 263 - 268, English

  [Refereed]

  Scientific journal


• CBPによるバイオエタノール生産技術の開発

  INOKUMA KENTARO, HASUNUMA TOMOHISA, KONDO AKIHIKO

  シーエムシー出版, May 2015, バイオインダストリー, 32(5) (5), 26 - 31, Japanese

  Scientific journal


• Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse

  Hideo Kawaguchi, Hiroshi Teramura, Kouji Uematsu, Kiyotaka Y. Hara, Tomohisa Hasunuma, Ko Hirano, Takashi Sazuka, Hidemi Kitano, Yota Tsuge, Prihardi Kahar, Satoko Niimi-Nakamura, Ken-Ichi Oinuma, Naoki Takaya, Shigemitsu Kasuga, Chiaki Ogino, Akihiko Kondo

  Apr. 2015, BIORESOURCE TECHNOLOGY, 182, 169 - 178, English

  [Refereed]

  Scientific journal


• Evaluation of genes involved in oxidative phosphorylation in yeast by developing a simple and rapid method to measure mitochondrial ATP synthetic activity

  Xiaoting Ye, Kana Morikawa, Shih-Hsin Ho, Michihiro Araki, Keiji Nishida, Tomohisa Hasunuma, Kiyotaka Y. Hara, Akihiko Kondo

  Apr. 2015, Microbial Cell Factories, 14, 56, English

  [Refereed]

  Scientific journal


• 代謝プロファイリングに基づく微生物育種技術の開発と応用

  HASUNUMA TOMOHISA

  日本生物工学会, Mar. 2015, 生物工学会誌, 93(3) (3), 122 - 129, Japanese

  Scientific journal


• Recent advances in the metabolic engineering of Corynebacterium glutamicum for the production of lactate and succinate from renewable resources

  Yota Tsuge, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2015, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 42(3) (3), 375 - 389, English

  [Refereed]

  Scientific journal


• Improved ethanol production from xylose in the presence of acetic acid by the overexpression of the HAA1 gene in Saccharomyces cerevisiae

  Yuri Sakihama, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2015, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 119(3) (3), 297 - 302, English

  [Refereed]

  Scientific journal


• Dynamic metabolic profiling of the marine microalga Chlamydomonas sp JSC4 and enhancing its oil production by optimizing light intensity

  Shih-Hsin Ho, Akihito Nakanishi, Xiaoting Ye, Jo-Shu Chang, Chun-Yen Chen, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2015, BIOTECHNOLOGY FOR BIOFUELS, 8, 48, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Hyperphosphorylation of DegU cancels CcpA-dependent catabolite repression of rocG in Bacillus subtilis

  Kosei Tanaka, Kana Iwasaki, Takuya Morimoto, Takatsugu Matsuse, Tomohisa Hasunuma, Shinji Takenaka, Onuma Chumsakul, Shu Ishikawa, Naotake Ogasawara, Ken-ichi Yoshida

  Feb. 2015, BMC MICROBIOLOGY, 15, 43, English

  [Refereed]

  Scientific journal


• Efficient co-displaying and artificial ratio control of alpha-amylase and glucoamylase on the yeast cell surface by using combinations of different anchoring domains

  Kentaro Inokuma, Takanobu Yoshida, Jun Ishii, Tomohisa Hasunuma, Akihiko Kondo

  Feb. 2015, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 99(4) (4), 1655 - 1663, English

  [Refereed]

  Scientific journal


• The impact of zinc sulfate addition on the dynamic metabolic profiling of Saccharomyces cerevisiae subjected to long term acetic acid stress treatment and identification of key metabolites involved in the antioxidant effect of zinc

  Chun Wan, Mingming Zhang, Qing Fang, Liang Xiong, Xinqing Zhao, Tomohisa Hasunuma, Fengwu Bai, Akihiko Kondo

  2015, METALLOMICS, 7(2) (2), 322 - 332, English

  [Refereed]

  Scientific journal


• Zinc, magnesium, and calcium ion supplementation confers tolerance to acetic acid stress in industrial Saccharomyces cerevisiae utilizing xylose

  Ku Syahidah Ku Ismail, Takatoshi Sakamoto, Tomohisa Hasunuma, Xin-Qing Zhao, Akihiko Kondo

  Dec. 2014, BIOTECHNOLOGY JOURNAL, 9(12) (12), 1519 - 1525, English

  [Refereed]

  Scientific journal


• Perspectives on engineering strategies for improving biofuel production from microalgae - A critical review

  Shih-Hsin Ho, Xiaoting Ye, Tomohisa Hasunuma, Jo-Shu Chang, Akihiko Kondo

  Dec. 2014, BIOTECHNOLOGY ADVANCES, 32(8) (8), 1448 - 1459, English

  [Refereed]

  Scientific journal


• Overexpression of flv3 improves photosynthesis in the cyanobacterium Synechocystis sp PCC6803 by enhancement of alternative electron flow

  Tomohisa Hasunuma, Mami Matsuda, Youhei Senga, Shimpei Aikawa, Masakazu Toyoshima, Ginga Shimakawa, Chikahiro Miyake, Akihiko Kondo

  Dec. 2014, BIOTECHNOLOGY FOR BIOFUELS, 7, 493, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Development of bio-based fine chemical production through synthetic bioengineering

  Kiyotaka Y. Hara, Michihiro Araki, Naoko Okai, Satoshi Wakai, Tomohisa Hasunuma, Akihiko Kondo

  Dec. 2014, MICROBIAL CELL FACTORIES, 13(1) (1), 173, English

  [Refereed]

  Scientific journal


• Optimized membrane process to increase hemicellulosic ethanol production from pretreated rice straw by recombinant xylose-fermenting Saccharomyces cerevisiae

  Kengo Sasaki, Yota Tsuge, Daisuke Sasaki, Tomohisa Hasunuma, Takatoshi Sakamoto, Yuri Sakihama, Chiaki Ogino, Akihiko Kondo

  Oct. 2014, BIORESOURCE TECHNOLOGY, 169, 380 - 386, English

  [Refereed]

  Scientific journal


• Development of a GIN11/FRT-based multiple-gene integration technique affording inhibitor-tolerant, hemicellulolytic, xylose-utilizing abilities to industrial Saccharomyces cerevisiae strains for ethanol production from undetoxified lignocellulosic hemicelluloses

  Tomohisa Hasunuma, Yoshimi Hori, Takatoshi Sakamoto, Misa Ochiai, Haruyo Hatanaka, Akihiko Kondo

  Oct. 2014, MICROBIAL CELL FACTORIES, 13(1) (1), 145, English

  [Refereed]

  Scientific journal


• Detoxification of furfural in Corynebacterium glutamicum under aerobic and anaerobic conditions

  Yota Tsuge, Yoshimi Hori, Motonori Kudou, Jun Ishii, Tomohisa Hasunuma, Akihiko Kondo

  Oct. 2014, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98(20) (20), 8675 - 8683, English

  [Refereed]

  Scientific journal


• A new screening method for recombinant Saccharomyces cerevisiae strains based on their xylose fermentation ability measured by near infrared spectroscopy

  Hiroyuki Morita, Tomohisa Hasunuma, Maria Vassileva, Akihiko Kondo, Roumiana Tsenkova

  Sep. 2014, ANALYTICAL METHODS, 6(17) (17), 6628 - 6634, English

  [Refereed]

  Scientific journal


• アーミング酵母による統合型バイオプロセスの開発

  HASUNUMA TOMOHISA, KONDO AKIHIKO

  To build an energy and material secure future, a next generation of renewable fuels and chemicals produced from lignocellulosic biomass is required. Since lignocellulosic biomass represents an abundant carbonneutral renewable resource for the production of biofuels, numerous environmental and social benefits could result from the replacement of petroleum-based transport fuels with bioethanol. However, the complicated ethanol production processes involved make the cost of producing bioethanol from it higher compared to corn starch and cane juice. Therefore, consolidated bioprocessing (CBP), which combines enzyme production, saccharification and fermentation in a single step, has gained increased recognition as a potential bioethanol production system. CBP requires a highly engineered microorganism developed for several different processspecific characteristics. The dominant strategy for engineering a CBP biocatalyst is to express multiple components of a cellulolytic system from either fungi or bacteria in the yeast Saccharomyces cerevisiae. The development of recombinant yeast strains displaying cellulases and hemicellulases on the cell surface, which are referred to as arming yeast strains, represents significant progress toward realization of CBP. The engineered yeast strains successfully perform the direct ethanol production from lignocellulosic biomass. The yeast is reusable for the hydrolysis and fermentation by collecting the cells. Reutilization of the cell-surface engineered yeast has an advantage in the reduction of enzyme cost, which enables reuse of enzymes on the cell surface. On the other hand, the difference in optimum temperature between saccharification and fermentation is a drawback of efficient ethanol production in the simultaneous saccharification and fermentation (SSF). The application of thermotolerant yeast strains engineered to the SSF process would overcome the drawback by performing hydrolysis and fermentation at elevated temperature.

  The Japan Institute of Energy, Jul. 2014, 日本エネルギー学会誌, 93(7) (7), 580 - 585, Japanese

  Scientific journal


• Simultaneous saccharification and fermentation of kraft pulp by recombinant Escherichia coli for phenyllactic acid production

  Hideo Kawaguchi, Kouji Uematsu, Chiaki Ogino, Hiroshi Teramura, Satoko Niimi-Nakamura, Yota Tsuge, Tomohisa Hasunuma, Ken-Ichi Oinuma, Naoki Takaya, Akihiko Kondo

  Jul. 2014, BIOCHEMICAL ENGINEERING JOURNAL, 88, 188 - 194, English

  [Refereed]

  Scientific journal


• Optimizing biodiesel production in marine Chlamydomonas sp JSC4 through metabolic profiling and an innovative salinity-gradient strategy

  Shih-Hsin Ho, Akihito Nakanishi, Xiaoting Ye, Jo-Shu Chang, Kiyotaka Hara, Tomohisa Hasunuma, Akihiko Kondo

  Jun. 2014, BIOTECHNOLOGY FOR BIOFUELS, 7, 97, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Glycogen production for biofuels by the euryhaline cyanobacteria Synechococcus sp strain PCC 7002 from an oceanic environment

  Shimpei Aikawa, Atsumi Nishida, Shih-Hsin Ho, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo

  Jun. 2014, BIOTECHNOLOGY FOR BIOFUELS, 7, 88, English

  [Refereed]

  Scientific journal


• Ethanol Production from Yeasts

  Tomohisa Hasunuma, Ryosuke Yamada, Akihiko Kondo

  Wiley Blackwell, Apr. 2014, Bioprocessing of Renewable Resources to Commodity Bioproducts, 201 - 226, English

  [Refereed]

  In book


• リグノセルロースからの高効率エタノール生産に向けた新規代謝改変酵母の創製

  HASUNUMA TOMOHISA, KONDO AKIHIKO

  To build an energy and material secure future, a next generation of renewable fuels and bio-based chemicals produced from lignocellulosic biomass is required. Consolidated bioprocessing (CBP), which integrates enzyme production, saccharification and fermentation into a single process, is a promising strategy for effective production of bio-based materials. As a key technology for the development of recombinant cellulolytic microbial strains, cell surface engineering, which enables the display through genetic engineering of various types of functional proteins on microbial cell surfaces without loss of their function, is a promising tool reducing the requirement for cellulase addition, as cellulases can be displayed on the microbial cell surface. Regardless of the process used for the biomass hydrolysis, CBP-enabling microorganisms encounter a variety of toxic compounds released during biomass pretreatment that inhibit microbial growth and ethanol yield. Systems biology approaches including transcriptomics and metabolomics have been recently exploited to gain insight into the molecular and genetic traits involved in tolerance and adaptation to the fermentation inhibitors. A combination of a cell surface displayed enzyme system and an intracellular metabolic engineering system is a very effective approach to develop cells with novel fermentation ability for industrial applications.

  Japan Oil Chemists' Society, Mar. 2014, オレオサイエンス, 14(3) (3), 95 - 101, Japanese

  Scientific journal


• Increased biomass production and glycogen accumulation in apcE gene deleted Synechocystis sp PCC 6803

  Ancy Joseph, Shimpei Aikawa, Kengo Sasaki, Fumio Matsuda, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2014, AMB EXPRESS, 4, 17, English

  [Refereed]

  Scientific journal


• Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural

  Tomohisa Hasunuma, Ku Syahidah Ku Ismail, Yumiko Nambu, Akihiko Kondo

  Feb. 2014, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 117(2) (2), 165 - 169, English

  [Refereed]

  Scientific journal


• Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp PCC 6803

  Joseph Ancy, Aikawa Shimpei, Sasaki Kengo, Teramura Hiroshi, Hasunuma Tomohisa, Matsuda Fumio, Osanai Takashi, Hirai Masami Yokota, Kondo Akihiko

  Jan. 2014, FEBS LETTERS, 588(3) (3), 466 - 471

  [Refereed]


• Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp PCC 6803

  Ancy Joseph, Shimpei Aikawa, Kengo Sasaki, Hiroshi Teramura, Tomohisa Hasunuma, Fumio Matsuda, Takashi Osanai, Masami Yokota Hirai, Akihiko Kondo

  Jan. 2014, FEBS LETTERS, 588(3) (3), 466 - 471, English

  [Refereed]

  Scientific journal


• Efficient yeast cell-surface display of exo- and endo-cellulase using the SED1 anchoring region and its original promoter

  Kentaro Inokuma, Tomohisa Hasunuma, Akihiko Kondo

  Jan. 2014, BIOTECHNOLOGY FOR BIOFUELS, 7(1) (1), 8, English

  [Refereed]

  Scientific journal


• Development of lipid productivities under different CO2 conditions of marine microalgae Chlamydomonas sp JSC4

  Akihito Nakanishi, Shimpei Aikawa, Shih-Hsin Ho, Chun-Yen Chen, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo

  Jan. 2014, BIORESOURCE TECHNOLOGY, 152, 247 - 252, English

  [Refereed]

  Scientific journal


• Respiration accumulates Calvin cycle intermediates for the rapid start of photosynthesis in Synechocystis sp. PCC 6803

  Ginga Shimakawa, Tomohisa Hasunuma, Akihiko Kondo, Mami Matsuda, Amane Makino, Chikahiro Miyake

  Japan Society for Bioscience Biotechnology and Agrochemistry, 2014, Bioscience, Biotechnology and Biochemistry, 78(12) (12), 1997 - 2007, English

  [Refereed]

  Scientific journal


• Direct production of organic acids from starch by cell surface-engineered Corynebacterium glutamicum in anaerobic conditions.

  Yota Tsuge, Toshihiro Tateno, Kengo Sasaki, Tomohisa Hasunuma, Tsutomu Tanaka, Akihiko Kondo

  We produced organic acids, including lactate and succinate, directly from soluble starch under anaerobic conditions using high cell-density cultures of Corynebacterium glutamicum displaying α-amylase (AmyA) from Streptococcus bovis 148 on the cell surface. Notably, reactions performed under anaerobic conditions at 35 and 40°C, which are higher than the optimal growth temperature of 30°C, showed 32% and 19%, respectively, higher productivity of the organic acids lactate, succinate, and acetate compared to that at 30°C. However, α-amylase was not stably anchored and released into the medium from the cell surface during reactions at these higher temperatures, as demonstrated by the 61% and 85% decreases in activity, respectively, from baseline, compared to the only 8% decrease at 30°C. The AmyA-displaying C. glutamicum cells retained their starch-degrading capacity during five 10 h reaction cycles at 30°C, producing 107.8 g/l of total organic acids, including 88.9 g/l lactate and 14.0 g/l succinate. The applicability of cell surface-engineering technology for the production of organic acids from biomass by high cell-density cultures of C. glutamicum under anaerobic conditions was demonstrated.

  Dec. 2013, AMB Express, 3(1) (1), 72 - 72, English, International magazine

  [Refereed]

  Scientific journal


• Ethanol fermentation by xylose-assimilating Saccharomyces cerevisiae using sugars in a rice straw liquid hydrolysate concentrated by nanofiltration

  Kengo Sasaki, Daisuke Sasaki, Yuri Sakihama, Hiroshi Teramura, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Nov. 2013, BIORESOURCE TECHNOLOGY, 147, 84 - 88, English

  [Refereed]

  Scientific journal


• Endowing non-cellulolytic microorganisms with cellulolytic activity aiming for consolidated bioprocessing

  Ryosuke Yamada, Tomohisa Hasunuma, Akihiko Kondo

  Nov. 2013, BIOTECHNOLOGY ADVANCES, 31(6) (6), 754 - 763, English

  [Refereed]

  Scientific journal


• Time-based comparative transcriptomics in engineered xylose-utilizing Saccharomyces cerevisiae identifies temperature-responsive genes during ethanol production

  Ku Syahidah Ku Ismail, Takatoshi Sakamoto, Tomohisa Hasunuma, Akihiko Kondo

  Sep. 2013, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 40(9) (9), 1039 - 1050, English

  [Refereed]

  Scientific journal


• Engineering strategies for improving the CO2 fixation and carbohydrate productivity of Scenedesmus obliquus CNW-N used for bioethanol fermentation

  Shih-Hsin Ho, Akihiko Kondo, Tomohisa Hasunuma, Jo-Shu Chang

  Sep. 2013, BIORESOURCE TECHNOLOGY, 143, 163 - 171, English

  [Refereed]

  Scientific journal


• Cocktail delta-integration of xylose assimilation genes for efficient ethanol production from xylose in Saccharomyces cerevisiae

  Hiroko Kato, Fumio Matsuda, Ryosuke Yamada, Kento Nagata, Tomokazu Shirai, Tomohisa Hasunuma, Akihiko Kondo

  Sep. 2013, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 116(3) (3), 333 - 336, English

  [Refereed]

  Scientific journal


• Aqueous size-exclusion chromatographic method for the quantification of cyanobacterial native glycogen

  Yoshihiro Izumi, Shimpei Aikawa, Fumio Matsuda, Tomohisa Hasunuma, Akihiko Kondo

  Jul. 2013, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 930, 90 - 97, English

  [Refereed]

  Scientific journal


• Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion

  HASUNUMA Tomohisa, KIKUYAMA F, MATSUDA M, AIKAWA S, IZUMI Y, KONDO Akihiko

  Jun. 2013, Journal of Experimental Botany, 64(10) (10), 2943 - 2954, English

  [Refereed]

  Scientific journal


• Detection of orally administered inositol stereoisomers in mouse blood plasma and their effects on translocation of glucose transporter 4 in skeletal muscle cells

  Yoko Yamashita, Masaru Yamaoka, Tomohisa Hasunuma, Hitoshi Ashida, Ken-Ichi Yoshida

  May 2013, Journal of Agricultural and Food Chemistry, 61(20) (20), 4850 - 4854, English

  [Refereed]

  Scientific journal


• Direct conversion of Spirulina to ethanol without pretreatment or enzymatic hydrolysis processes

  AIKAWA S, JOSEPH A, YAMADA R, IZUMI Y, YAMAGISHI T, MATSUDA F, KAWAI H, CHANG JS, HASUNUMA Tomohisa, KONDO Akihiko

  May 2013, Energy & Environmental Science, 6(6) (6), 1844 - 1849, English

  [Refereed]

  Scientific journal


• Cell recycle batch fermentation of high-solid lignocellulose using a recombinant cellulase-displaying yeast strain for high yield ethanol production in consolidated bioprocessing

  Yuki Matano, Tomohisa Hasunuma, Akihiko Kondo

  May 2013, BIORESOURCE TECHNOLOGY, 135, 403 - 409, English

  [Refereed]

  Scientific journal


• Bioethanol production, using carbohydrate-rich microalgae biomass as feedstock

  Shih-Hsin Ho, Shu-Wen Huang, Chun-Yen Chen, Tomohisa Hasunuma, Akihiko Kondo, Jo-Shu Chang

  May 2013, BIORESOURCE TECHNOLOGY, 135, 191 - 198, English

  [Refereed]

  Scientific journal


• A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of their application to consolidated bioprocessing technology

  Tomohisa Hasunuma, Fumiyoshi Okazaki, Naoko Okai, Kiyotaka Y. Hara, Jun Ishii, Akihiko Kondo

  May 2013, BIORESOURCE TECHNOLOGY, 135, 513 - 522, English

  [Refereed]

  Scientific journal


• Simultaneous improvement of saccharification and ethanol production from crystalline cellulose by alleviation of irreversible adsorption of cellulase with a cell surface-engineered yeast strain

  Yuki Matano, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2013, Applied Microbiology and Biotechnology, 97(5) (5), 2231 - 2237, English

  [Refereed]

  Scientific journal


• Reduction of furan derivatives by overexpressing NADH-dependent Adh1 improves ethanol fermentation using xylose as sole carbon source with Saccharomyces cerevisiae harboring XR-XDH pathway

  Jun Ishii, Kazuya Yoshimura, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2013, Applied Microbiology and Biotechnology, 97(6) (6), 2597 - 2607, English

  [Refereed]

  Scientific journal


• Implementation of a transhydrogenase-like shunt to counter redox imbalance during xylose fermentation in Saccharomyces cerevisiae

  Hiroyuki Suga, Fumio Matsuda, Tomohisa Hasunuma, Jun Ishii, Akihiko Kondo

  Feb. 2013, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(4) (4), 1669 - 1678, English

  [Refereed]

  Scientific journal


• Ability of a perfluoropolymer membrane to tolerate by-products of ethanol fermentation broth from dilute acid-pretreated rice straw

  Kengo Sasaki, Fumio Matsuda, Tomohisa Hasunuma, Chiaki Ogino, Masakatsu Urairi, Kazuhito Yoshida, Akihiko Kondo

  Jan. 2013, Biochemical Engineering Journal, 70, 135 - 139, English

  [Refereed]

  Scientific journal


• Gene expression cross-profiling in genetically modified industrial Saccharomyces cerevisiae strains during high-temperature ethanol production from xylose

  Ku Syahidah Ku Ismail, Takatoshi Sakamoto, Haruyo Hatanaka, Tomohisa Hasunuma, Akihiko Kondo

  Jan. 2013, Journal of Biotechnology, 163(1) (1), 50 - 60, English

  [Refereed]

  Scientific journal


• Development of microbial cell factories for bio-refinery through synthetic bioengineering

  Akihiko Kondo, Jun Ishii, Kiyotaka Y. Hara, Tomohisa Hasunuma, Fumio Matsuda

  Jan. 2013, JOURNAL OF BIOTECHNOLOGY, 163(2) (2), 204 - 216, English

  [Refereed]

  Scientific journal


• Characterization and optimization of carbohydrate production from an indigenous microalga Chlorella vulgaris FSP-E

  Shih-Hsin Ho, Shu-Wen Huang, Chun-Yen Chen, Tomohisa Hasunuma, Akihiko Kondo, Jo-Shu Chang

  Elsevier Ltd, 2013, Bioresource Technology, 135, 157 - 165, English

  [Refereed]

  Scientific journal


• Development of yeast cell factories for consolidated bioprocessing of lignocellulose to bioethanol through cell surface engineering

  Tomohisa Hasunuma, Akihiko Kondo

  Nov. 2012, BIOTECHNOLOGY ADVANCES, 30(6) (6), 1207 - 1218, English

  [Refereed]

  Scientific journal


• バイオリファイナリーとバイオプラスチック

  OKAI NAOKO, HASUNUMA TOMOHISA, KONDO AKIHIKO

  石油学会 ; 1978-, Oct. 2012, ペトロテック, 35(10) (10), 700 - 706, Japanese

  Scientific journal


• Consolidated bioprocessing and simultaneous saccharification and fermentation of lignocellulose to ethanol with thermotolerant yeast strains

  Tomohisa Hasunuma, Akihiko Kondo

  Sep. 2012, PROCESS BIOCHEMISTRY, 47(9) (9), 1287 - 1294, English

  [Refereed]

  Scientific journal


• Design of superior cell factories for a sustainable biorefinery by synthetic bioengineering

  Tomohisa Hasunuma, Fumio Matsuda, Akihiko Kondo

  Springer Netherlands, Aug. 2012, Systems Metabolic Engineering, 9789400745346, 329 - 348, English

  [Refereed]

  In book


• 合成生物工学によるバイオ燃料生産のための微生物細胞工場の創製

  HASUNUMA TOMOHISA, KONDO AKIHIKO

  日本生物工学会, Jul. 2012, 生物工学会誌, 90(7) (7), 386 - 391, Japanese

  Scientific journal


• Improvements in ethanol production from xylose by mating recombinant xylose-fermenting Saccharomyces cerevisiae strains

  Hiroko Kato, Hiroaki Suyama, Ryosuke Yamada, Tomohisa Hasunuma, Akihiko Kondo

  Jun. 2012, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 94(6) (6), 1585 - 1592, English

  [Refereed]

  Scientific journal


• Widely targeted metabolic profiling analysis of yeast central metabolites

  Hiroko Kato, Yoshihiro Izumi, Tomohisa Hasunuma, Fumio Matsuda, Akihiko Kondo

  May 2012, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 113(5) (5), 665 - 673, English

  [Refereed]

  Scientific journal


• Deletion of the PHO13 gene in Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysate in the presence of acetic and formic acids, and furfural

  Keisuke Fujitomi, Tomoya Sanda, Tomohisa Hasunuma, Akihiko Kondo

  May 2012, BIORESOURCE TECHNOLOGY, 111, 161 - 166, English

  [Refereed]

  Scientific journal


• Direct ethanol production from hemicellulosic materials of rice straw by use of an engineered yeast strain codisplaying three types of hemicellulolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells

  Takatoshi Sakamoto, Tomohisa Hasunuma, Yoshimi Hori, Ryosuke Yamada, Akihiko Kondo

  Apr. 2012, JOURNAL OF BIOTECHNOLOGY, 158(4) (4), 203 - 210, English

  [Refereed]

  Scientific journal


• Synergistic enhancement of glycogen production in Arthrospira platensis by optimization of light intensity and nitrate supply

  Shimpei Aikawa, Yoshihiro Izumi, Fumio Matsuda, Tomohisa Hasunuma, Jo-Shu Chang, Akihiko Kondo

  Mar. 2012, BIORESOURCE TECHNOLOGY, 108, 211 - 215, English

  [Refereed]

  Scientific journal


• Display of cellulases on the cell surface of Saccharomyces cerevisiae for high yield ethanol production from high-solid lignocellulosic biomass

  Yuki Matano, Tomohisa Hasunuma, Akihiko Kondo

  Mar. 2012, BIORESOURCE TECHNOLOGY, 108, 128 - 133, English

  [Refereed]

  Scientific journal


• Repeated-batch fermentation of lignocellulosic hydrolysate to ethanol using a hybrid Saccharomyces cerevisiae strain metabolically engineered for tolerance to acetic and formic acids

  Tomoya Sanda, Tomohisa Hasunuma, Fumio Matsuda, Akihiko Kondo

  Sep. 2011, BIORESOURCE TECHNOLOGY, 102(17) (17), 7917 - 7924, English

  [Refereed]

  Scientific journal


• Variation in Biomass Properties among Rice Diverse Cultivars

  Fumio Matsuda, Masanori Yamasaki, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  Aug. 2011, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 75(8) (8), 1603 - 1605, English

  [Refereed]

  Scientific journal


• Near Infrared Spectroscopy As High-Throughput Technology for Screening of Xylose-Fermenting Recombinant Saccharomyces cerevisiae Strains

  Hiroyuki Morita, Tomohisa Hasunuma, Maria Vassileva, Roumiana Tsenkova, Akihiko Kondo

  Jun. 2011, ANALYTICAL CHEMISTRY, 83(11) (11), 4023 - 4029, English

  [Refereed]

  Scientific journal


• Efficient fermentation of xylose to ethanol at high formic acid concentrations by metabolically engineered Saccharomyces cerevisiae

  Tomohisa Hasunuma, Kyung-mo Sung, Tomoya Sanda, Kazuya Yoshimura, Fumio Matsuda, Akihiko Kondo

  May 2011, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 90(3) (3), 997 - 1004, English

  [Refereed]

  Scientific journal


• Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae

  Tomohisa Hasunuma, Tomoya Sanda, Ryosuke Yamada, Kazuya Yoshimura, Jun Ishii, Akihiko Kondo

  Jan. 2011, MICROBIAL CELL FACTORIES, 10, English

  [Refereed]

  Scientific journal


• Construction of a xylose-metabolizing yeast by genome integration of xylose isomerase gene and investigation of the effect of xylitol on fermentation

  Takanori Tanino, Atsushi Hotta, Tomonori Ito, Jun Ishii, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Naoto Ohmura, Takayuki Ohshima, Akihiko Kondo

  Nov. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 88(5) (5), 1215 - 1221, English

  [Refereed]

  Scientific journal


• Construction of a xylose-metabolizing Saccharomyces cerevisiae by integration of xylose isomerase gene into the genome and investigation of the effect of xylITOl on fermentation

  Takanori Tanino, Atsushi Hotta, Tomonori Ito, Jun Ishii, Ryosuke Yamada, Tomohisa Hasunuma, Chiaki Ogino, Naoto Ohmura, Takayuki Ohima, Akihiko Kondo

  Nov. 2010, Applied Microbiology and Biotechnology, 88, 1215-1221, English

  [Refereed]

  Scientific journal


• Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes

  Shuhei Yanase, Tomohisa Hasunuma, Ryosuke Yamada, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  Sep. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 88(1) (1), 381 - 388, English

  [Refereed]

  Scientific journal


• Co-fermentation of cellobiose and xylose using beta-glucosidase displaying diploid industrial yeast strain OC-2

  Satoshi Saitoh, Tomohisa Hasunuma, Tsutomu Tanaka, Akihiko Kondo

  Aug. 2010, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(5) (5), 1975 - 1982, English

  [Refereed]

  Scientific journal


• Ethanol production from cellulosic materials using cellulase-expressing yeast

  Shuhei Yanase, Ryosuke Yamada, Shohei Kaneko, Hideo Noda, Tomohisa Hasunuma, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

  May 2010, BIOTECHNOLOGY JOURNAL, 5(5) (5), 449 - 455, English

  [Refereed]

  Scientific journal


• Metabolic turnover analysis by a combination of in vivo C-13-labelling from (CO2)-C-13 and metabolic profiling with CE-MS/MS reveals rate-limiting steps of the C-3 photosynthetic pathway in Nicotiana tabacum leaves

  Tomohisa Hasunuma, Kazuo Harada, Shin-Ichi Miyazawa, Akihiko Kondo, Eiichiro Fukusaki, Chikahiro Miyake

  Mar. 2010, JOURNAL OF EXPERIMENTAL BOTANY, 61(4) (4), 1041 - 1051, English

  [Refereed]

  Scientific journal


• Metabolic pathway engineering by plastid transformation is a powerful tool for production of compounds in higher plants

  Tomohisa Hasunuma, Akihiko Kondo, Chikahiro Miyake

  Mar. 2009, PLANT BIOTECHNOLOGY, 26(1) (1), 39 - 46, English

  [Refereed]

  Scientific journal


• キシロースからの効率的なエタノール生産を行う酵母の創製

  加藤 寛子, 山田 亮祐, 蓮沼 誠久, 福田 秀樹, 近藤 昭彦

  公益社団法人 化学工学会, 2009, 化学工学会 研究発表講演要旨集, 2009, 4 - 4, Japanese


• Biosynthesis of astaxanthin in tobacco leaves by transplastomic engineering

  Tomohisa Hasunuma, Shin-Ichi Miyazawa, Satomi Yoshimura, Yuki Shinzaki, Ken-Ichi Tomizawa, Kazutoshi Shindo, Seon-Kang Choi, Norihiko Misawa, Chikahiro Miyake

  Sep. 2008, PLANT JOURNAL, 55(5) (5), 857 - 868, English

  [Refereed]

  Scientific journal


• Overexpression of 1-deoxy-D-xylulose-5-phosphate reductoisomerase gene in chloroplast contributes to increment of isoprenoid production

  Tomohisa Hasunuma, Shinya Takeno, Shunsuke Hayashi, Mayumi Sendai, Takeshi Bamba, Satomi Yoshimura, Ken-ichi Tomizawa, Eiichiro Fukusaki, Chikahiro Miyake

  May 2008, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 105(5) (5), 518 - 526, English

  [Refereed]

  Scientific journal


• 4-Ketoantheraxanthin, a novel carotenoid produced by the combination of the bacterial enzyme b-carotene ketolase CrtW and endogeneous carotenoid biosynthetic enzymes in hihger plants

  SHINDO Kazutoshi, HASUNUMA Tomohisa, ASAGI Emiko, SANO Aya, HOTTA Eri, MINEMURA Noriko, MIYAKE Chikahiro, MAOKA Takashi, MISAWA Norihiko

  Mar. 2008, Tetrahedron Letters, Vol 49. No. 20, pp. 3294-3296, English

  [Refereed]

  Scientific journal


• Expression of fungal pectin methylesterase in transgenic tobacco leads to alteration in cell wall metabolism and a dwarf phenotype

  T Hasunuma, E Fukusaki, A Kobayashi

  Aug. 2004, JOURNAL OF BIOTECHNOLOGY, 111(3) (3), 241 - 251, English

  [Refereed]

  Scientific journal


• Real-time quantification of methanol in plants using a hybrid alcohol oxidase-peroxidase biosensor

  T Hasunuma, S Kuwabata, E Fukusaki, A Kobayashi

  Mar. 2004, ANALYTICAL CHEMISTRY, 76(5) (5), 1500 - 1506, English

  [Refereed]

  Scientific journal


• Methanol production is enhanced by expression of an Aspergillus niger pectin methylesterase in tobacco cells

  T Hasunuma, E Fukusaki, A Kobayashi

  Dec. 2003, JOURNAL OF BIOTECHNOLOGY, 106(1) (1), 45 - 52, English

  [Refereed]

  Scientific journal


• SELEX for tubulin affords specific T-rich DNA aptamers

  E Fukusaki, T Hasunuma, S Kajiyama, A Okazawa, TJ Itoh, A Kobayashi

  Nov. 2001, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 11(22) (22), 2927 - 2930, English

  [Refereed]

  Scientific journal

• プロポリス生理活性成分アルテピリンCの酵母による生産

  番場崇弘, 棟方涼介, 矢﨑一史, 蓮沼誠久

  May 2025, バイオサイエンスとインダストリー, 83(3) (3), 246 - 247


• "Manufacturing" bioresources using AI and genome-edited microorganisms

  蓮沼誠久

  Jan. 2025, HEALTHIS, (289) (289), 14 - 17


• Photosynthetic production of glutamine by metabolic analysis-based engineering of Synechococcus sp.

  加藤悠一, 辻彩花, 原口裕次, 清水達也, 近藤昭彦, 蓮沼誠久, 近藤昭彦, 蓮沼誠久

  2025, 日本生物工学会大会講演要旨集, 77th


• ppGpp に着目した細胞増殖制御による光合成的芳香族生産

  蓮沼誠久

  Oct. 2024, BIO INDUSTRY, 41(10) (10), 1 - 7


• ppGppによるラン藻の窒素利用制御

  蓮沼誠久

  Sep. 2024, 光アライアンス, 35(9) (9), 18 - 21


• バイオエコノミーの実現に向けたバイオものづくりの推進における課題と政策的検討を行う上での必須事項に関するフレームワーク

  松尾真紀子, 松本拓郎, 石塚大輔, 吉田彩乃, 城山英明, 蓮沼誠久, 立川雅司, 水無渉

  Sep. 2024, 東京大学未来ビジョン研究センター、ワーキングペーパーNo.32


• 光合成微生物の代謝ダイナミクス解析—Analyses of metabolic dynamics in photosynthetic microbes

  田中 謙也, 蓮沼 誠久

  東京 : 北隆館, Jul. 2024, アグリバイオ = Agricultural biotechnology, 8(8) (8), 691 - 695, Japanese


• 産業と行政 バイオものづくりに資するテンプレート酵素の探索と応用

  蓮沼 誠久

  2024, バイオサイエンスとインダストリー, 82(4) (4), 432 - 434


• Development of aldehyde-deformylating oxygenase with substrate specificity toward medium-chain aldehydes by computational enzyme design

  工藤恒, 近藤昭彦, 蓮沼誠久

  2024, 日本生物工学会大会講演要旨集, 76th


• Carbon Resource Partitioning in Microalgae and Cyanobacteria Quantitatively Elucidated by Metabolome Analysis

  加藤悠一, 加藤悠一, 蓮沼誠久, 蓮沼誠久

  2023, 日本植物生理学会年会(Web), 64th


• Mutational breeding and analysis of an induced glyceride accumulation-deficient strain in Chlamydomonas reinhardtii

  加藤悠一, 加藤悠一, 蓮沼誠久, 蓮沼誠久

  2023, 日本生物工学会大会講演要旨集, 75th


• Maintaining downstream glycolytic intermediates ensures rapid start of photosynthesis in cyanobacteria

  田中謙也, 田中謙也, 田中謙也, 松田真実, 白井智量, 白井智量, 近藤昭彦, 近藤昭彦, 近藤昭彦, 蓮沼誠久, 蓮沼誠久, 蓮沼誠久

  2023, 日本植物生理学会年会(Web), 64th


• Metabolism for photosynthetic induction in cyanobacteria

  田中謙也, 蓮沼誠久

  2023, 光合成研究, 33(1) (1)


• 特集 CO2から有用物質を生産し低炭素社会実現へ：AIと代謝工学を組み合わせ生産株開発を加速

  蓮沼誠久

  Jan. 2023, JST news, 1, 10 - 11, Japanese


• 第4章 生物工学のこれから これからの生物育種 -代謝工学による育種のこれから-

  蓮沼誠久

  Oct. 2022, 日本生物工学会100年史, 58 - 60, Japanese

  Introduction scientific journal


• 第3章 生物工学の研究100年 育種技術 -突然変異から代謝工学へ-

  近藤昭彦, 蓮沼誠久, 秀瀬涼太

  Oct. 2022, 日本生物工学会100年史, 29 - 31, Japanese

  Introduction scientific journal


• 微生物の高速育種を実現するスマートセル創出プラットフォーム

  蓮沼誠久, 秀瀬涼太, 番場崇弘

  Apr. 2022, 化学工学 [特集]「生物機能を利用したモノづくり」に貢献するプロセス強化, 86(4) (4), 157 - 160, Japanese

  Introduction scientific journal


• 進化する酵母細胞表層工学 -バイオインターフェイスの高度利用に向けた技術戦略-

  猪熊健太郎, 蓮沼誠久

  Mar. 2022, オレオサイエンス, 22(3) (3), 99 - 105, Japanese

  Introduction scientific journal


• Development of high fucoxanthin production technology using Haptophyta Pavlova sp. based on metabolome knowledges

  吉田江里菜, 加藤悠一, 金本昭彦, 近藤昭彦, 近藤昭彦, 近藤昭彦, 蓮沼誠久, 蓮沼誠久

  2022, 日本生物工学会大会講演要旨集, 74th


• Development of novel cyanobacteria with L-lactate utilization ability

  加藤悠一, 稲辺宏輔, 辻彩花, 原口裕次, 清水達也, 近藤昭彦, 近藤昭彦, 蓮沼誠久, 蓮沼誠久

  2022, 日本生物工学会大会講演要旨集, 74th


• A quantitative characterization of metabolic dynamics during photosynthesis start in cyanobacteria

  田中謙也, 田中謙也, 松田真実, 白井智量, 蓮沼誠久, 蓮沼誠久

  2022, 日本植物生理学会年会(Web), 63rd


• A quantitative characterization of rapid metabolic dynamics during activation of Calvin cycle in cyanobacteria

  田中謙也, 白井智量, 白井智量, 松田真実, 近藤昭彦, 近藤昭彦, 近藤昭彦, 蓮沼誠久, 蓮沼誠久, 蓮沼誠久

  2022, 日本生物工学会大会講演要旨集, 74th


• Development of aldehyde-deformylating oxygenase with substrate specificificity toward various carbon chain length aldehydes

  工藤恒, VAVRICKA Christopher J., 伏見圭司, 蓮沼誠久, 近藤昭彦

  2022, 日本生物工学会大会講演要旨集, 74th


• Development of alpha-keto acid decarboxylase for tailor-made bio-production

  伏見圭司, 秀瀬涼太, 秀瀬涼太, VAVRICKA Christopher J., 工藤恒, 蓮沼誠久, 蓮沼誠久, 近藤昭彦, 近藤昭彦

  2022, 日本生物工学会大会講演要旨集, 74th


• 光合成メタボロミクスの物質生産への応用

  加藤悠一, 秀瀬涼太, 蓮沼誠久

  Sep. 2021, 生物工学会誌, 99(9) (9), 456 - 460, Japanese


• 特集 藻類バイオマス利用のための新しい生物工学

  林勇樹, 工藤恒, 新井宗仁, 新井宗仁

  Aug. 2021, 生物工学会誌, 99(8) (8), 403 - 403, Japanese


• Development of a novel fucoxanthin production technology using Haptophyta Pavlova sp. based on the comprehensive metabolic profiling

  吉田江里菜, 加藤悠一, 金本昭彦, 蓮沼誠久, 近藤昭彦

  2021, 日本農芸化学会大会講演要旨集(Web), 2021


• Highly-accurate quantification of NADP(H) in cyanobacteria

  田中謙也, 田中謙也, 嶋川銀河, 嶋川銀河, 草間翔子, 松田真実, 蓮沼誠久, 蓮沼誠久, 中西周次

  2021, 日本生物工学会大会講演要旨集, 73rd


• 鉄代謝改変酵母による1,2,4-ブタントリオール生産

  番場崇弘, 湯川貴弘, 蓮沼誠久

  Mar. 2020, バイオサイエンスとインダストリー, 78(2) (2), 114 - 115, Japanese

  Introduction scientific journal


• スマートセルインダストリーの形成に向けたバイオ×デジタルの技術開発

  蓮沼 誠久

  Feb. 2020, 化学工学, 84(2) (2), 51, Japanese

  Introduction scientific journal


• 新たに設計した代謝経路を用いた大腸菌によるアルカロイド高生産

  蓮沼 誠久, Christopher John Vavricka Jr., 荒木通啓

  Jan. 2020, バイオサイエンスとインダストリー, 78(1) (1), 32 - 33, Japanese

  Introduction scientific journal


• スマートセル開発に資するメタボロミクス技術

  蓮沼 誠久

  Sep. 2019, バイオサイエンスとインダストリー, 77(5) (5), 410 - 411, Japanese

  Introduction scientific journal


• 細胞表層提示酵母を利用したクラフトパルプからのキシリトール生産

  番場 崇弘, 猪熊 健太郎, Gregory Guirimand, 蓮沼 誠久

  Sep. 2019, バイオサイエンスとインダストリー, 77(5) (5), 390 - 392, Japanese

  Introduction scientific journal


• Mechanism-based tuning of insect 3,4-dihydroxyphenylacetaldehyde synthase for synthetic bioproduction of benzylisoquinoline alkaloids.

  Christopher J Vavricka, Takanobu Yoshida, Yuki Kuriya, Shunsuke Takahashi, Teppei Ogawa, Fumie Ono, Kazuko Agari, Hiromasa Kiyota, Jianyong Li, Jun Ishii, Kenji Tsuge, Hiromichi Minami, Michihiro Araki, Tomohisa Hasunuma, Akihiko Kondo

  Previous studies have utilized monoamine oxidase (MAO) and L-3,4-dihydroxyphenylalanine decarboxylase (DDC) for microbe-based production of tetrahydropapaveroline (THP), a benzylisoquinoline alkaloid (BIA) precursor to opioid analgesics. In the current study, a phylogenetically distinct Bombyx mori 3,4-dihydroxyphenylacetaldehyde synthase (DHPAAS) is identified to bypass MAO and DDC for direct production of 3,4-dihydroxyphenylacetaldehyde (DHPAA) from L-3,4-dihydroxyphenylalanine (L-DOPA). Structure-based enzyme engineering of DHPAAS results in bifunctional switching between aldehyde synthase and decarboxylase activities. Output of dopamine and DHPAA products is fine-tuned by engineered DHPAAS variants with Phe79Tyr, Tyr80Phe and Asn192His catalytic substitutions. Balance of dopamine and DHPAA products enables improved THP biosynthesis via a symmetrical pathway in Escherichia coli. Rationally engineered insect DHPAAS produces (R,S)-THP in a single enzyme system directly from L-DOPA both in vitro and in vivo, at higher yields than that of the wild-type enzyme. However, DHPAAS-mediated downstream BIA production requires further improvement.

  01 May 2019, Nature communications, 10(1) (1), 2015 - 2015, English, International magazine

  Link to Kobe Univ. Repository (Kernel)


• Branch Spirit

  蓮沼 誠久

  Mar. 2019, 生物工学会誌, 97(3) (3), 150 - 151, Japanese

  Introduction scientific journal


• チャンスを掴むための準備, バイオ系のキャリアデザイン

  HASUNUMA TOMOHISA

  Oct. 2018, 生物工学会, 96(10) (10), 598 - 601, Japanese

  Introduction scientific journal


• バイオリファイナリー実現を加速する先端バイオ技術と情報技術の融合

  HASUNUMA TOMOHISA

  Nov. 2017, アグリバイオ, 1(12) (12), 24 - 29, Japanese

  Introduction scientific journal


• Complete Genome Sequence of Kluyveromyces marxianus NBRC1777, a Nonconventional Thermotolerant Yeast (vol 3, e00389-15, 2015)

  Kentaro Inokuma, Jun Ishii, Kiyotaka Y. Hara, Masao Mochizuki, Tomohisa Hasunuma, Akihiko Kondo

  Feb. 2017, MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 5(5) (5), English

  Others


• 多彩な戦略で挑むシアノバクテリア由来の燃料生産 持続可能な第三世代バイオ燃料生産の最前線

  日原 由香子, 朝山 宗彦, 蘆田 弘樹, 天尾 豊, 新井 宗仁, 栗井 光一郎, 得平 茂樹, 小山内 崇, 鞆 達也, 成川 礼, 蓮沼 誠久, 増川 一

  日本農芸化学会 ; 1962-, Feb. 2017, 化学と生物, 55(2) (2), 88 - 97, Japanese

  Introduction scientific journal


• バイオ燃料生産に向けた耐塩性緑藻の進化工学的育種と塩ストレス耐性による油脂生産低下メカニズムの解明

  加藤悠一, HO Shih-Hsin, CHANG Jo-Shu, 蓮沼誠久, 近藤昭彦

  2017, 日本農芸化学会大会講演要旨集(Web), 2017


• 微細藻類を利用したバイオ燃料とカロテノイド類の生産

  加藤 悠一, 蓮沼 誠久

  レーザー学会, Nov. 2016, レーザー研究, 11(11) (11), 731 - 734, Japanese

  Introduction scientific journal


• 原子間力顕微鏡による酵母細胞表層セルラーゼの局在評価

  InokumaKentarou, TAKENAKA Musashi, OGINO Chiaki, Hasunuma Tomohisa, KONDO Akihiko

  日本生物工学会, Nov. 2016, 生物工学, 94(11) (11), 698 - 700, Japanese

  [Refereed]

  Introduction scientific journal


• 次世代バイオエタノール製造技術

  InokumaKentarou, Hasunuma Tomohisa, KONDO Akihiko

  シーエムシー出版, Oct. 2016, バイオマスエネルギーの技術と市場, 第4章, 33 - 45, Japanese

  [Refereed]

  Introduction scientific journal


• 代謝プロファイリング法の微生物育種技術への応用

  蓮沼 誠久

  2016, Journal of Environmental Biotechnology, 16(1) (1), 51 - 58, Japanese

  Introduction scientific journal


• MALDI-TOF/MSを用いた発現タンパク質プロファイル測定方法の開発

  原清敬, 望月正雄, 蓮沼誠久, 中津井雅彦, 荒木通啓, 近藤昭彦

  2015, 日本ゲノム微生物学会年会要旨集, 9th


• 2S-Ca04 Development of microbial cell factories for biorefinery

  Tomohisa Hasunuma, Akihiko Kondo

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 250 - 250, English


• 1P-179 Development of biofuel production from chitinous substrates

  Inokuma Kentaro, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 133 - 133, Japanese


• 1P-206 Combined cell-surface display- and secretion-based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae :

  Liu Zhuo, Inokuma Kentaro, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2015, 日本生物工学会大会講演要旨集, 67, 140 - 140, English


• 好気・低濃度グルコース条件下におけるSaccharomyces cerevisiaeの転写解析

  崎濱由梨, 蓮沼誠久, 蓮沼誠久, 近藤昭彦, 近藤昭彦

  2015, 日本生物工学会大会講演要旨集, 67th


• Saccharomyces cerevisiaeの糖代謝における転写制御ネットワークの予測

  南部由美子, 南部由美子, 崎濱由梨, 荒木道啓, 蓮沼誠久, 近藤昭彦, 近藤昭彦

  2015, 日本生物工学会大会講演要旨集, 67th


• 3P-045 Effects of hydrolysate of lignocellulosic biomass on phenyllactate fermentation by recombinant Escherichia coli

  Kawaguchi Hideo, Teramura Hiroshi, Niimi-Nakamura Satoko, Ogino Chiaki, Hara Kiyotaka, Hasunuma Tomohisa, Oinuma Ken-Ichi, Takaya Naoki, Hirano Ko, Sazuka Takashi, Kitano Hidemi, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 206 - 206, Japanese


• 3P-044 Improvement of CHO cells useful in bio-pharmaceutical development, construction of data leading to the culture state elucidation

  Iga Tomoyo, Hasunuma Tomohisa, Araki Michihiro, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 205 - 205, Japanese


• 1P-134 Development of the novel secretion signal sequence for highly-efficient cell surface display and secretory production of proteins by yeasts

  Inokuma Kentaro, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 51 - 51, Japanese


• 3P-205 Metabolite profiling of oceanic cyanobacteria under salt stress condition

  Nishida Atsumi, Aikawa Shimpei, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 246 - 246, Japanese


• 3P-229 Effect of PHO13 gene disruption on xylose production in xylose-fermenting yeast harboring xylose isomerase gene

  Bamba Takahiro, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 252 - 252, Japanese


• 2P-132 Development of lipid-producing system depending on estimation of cell composition and metabolic analysis for marine green alga Chlamydomonas sp. JSC4

  Nakanishi Akihito, Ho Shih-Hsin, Chang Jo-Shu, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 139 - 139, Japanese


• 2P-136 Effect of illumination coupled with nitrogen depletion on biodiesel production of a marine microalga Chlamydomonas sp. JSC4 :

  Ho Shih-Hsin, Nakanishi Akihito, Ye Xiaoting, Chang Jo-Shu, Hara Kiyotaka, Kondo Akihiko, Hasunuma Tomohisa

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 140 - 140, English


• 2P-095 High-level ethanol production from cyanobacterium Spirulina by amylase displayed yeast

  Aikawa Shimpei, Inokuma Kentaro, Sasaki Kengo, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 130 - 130, Japanese


• 2A-Dp01 Development of microbe breeding technology based on metabolic profiling

  Hasunuma Tomohisa

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 98 - 98, Japanese


• 1P-110 Detoxification of furfural in Corynebacterium glutamicum under aerobic and anaerobic conditions

  Tsuge Yota, Hori Yoshimi, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 44 - 44, Japanese


• 3S-Ba05 Challenge for liquid fuel production from algae based on metabolic analysis

  Hasunuma Tomohisa

  日本生物工学会, 2014, 日本生物工学会大会講演要旨集, 66, 177 - 177, Japanese


• キシロース資化性酵母における遺伝子発現プロファイルの経時変化解析

  南部由美子, 崎濱由梨, 蓮沼誠久, 蓮沼誠久, 近藤昭彦, 近藤昭彦

  2014, 日本生物工学会大会講演要旨集, 66th


• Kluyveromyces marxianusの好気条件での糖代謝系の解析

  崎濱由梨, 蓮沼誠久, 蓮沼誠久, 近藤昭彦, 近藤昭彦

  2014, 日本生物工学会大会講演要旨集, 66th


• バイオリファイナリー社会に向けた燃料・化学品生産

  ISHII JUN, HASUNUMA TOMOHISA, MATSUDA FUMIO, KONDO AKIHIKO

  Aug. 2013, 安全工学, 52(4) (4), 249 - 255, Japanese

  Introduction scientific journal


• 革新的なものづくり実現のための「合成生物工学」

  ISHII JUN, HASUNUMA TOMOHISA, MATSUDA FUMIO, KONDO AKIHIKO

  日本生物工学会, Jun. 2013, 生物工学会誌, 91(6) (6), 314 - 318, Japanese

  Introduction scientific journal


• 1P-104 Identification of genes related to improved ethanol production from xylose in presence of acetate and formate by regulation of metabolism in Saccharomyces cerevisiae

  Sakamoto Takatoshi, Hori Yoshimi, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 43 - 43, Japanese


• 1P-185 Development of high lipid producing system by green alga Chlamydomonas orbicularis under sea salt condition

  Nakanishi Akihito, Ho Shih-Hsin, Aikawa Shimpei, Chang Jo-Shu, Kondo Akihiko, Hasunuma Tomohisa

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 64 - 64, Japanese


• 1P-196 Phototrophic cultivation of a marine microalga Chlamydomonas orbicularis for CO_2 fixation and biodiesel production: Effect of medium composition, nitrogen depletion, and sea salt concentration :

  Ho Shih-Hsin, Nakanishi Akihito, Aikawa Shimpei, Chang Jo-Shu, Kondo Akihiko, Hasunuma Tomohisa

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 66 - 66, English


• 1P-186 Glycogen production for biorefinery using oceanic cyanobacteria

  Nishida Atsumi, Aikawa Shimpei, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 64 - 64, Japanese


• 1P-146 Improvement of ethanol production from xylose by regulation of the PHO pathway in Saccharomyces cerevisiae

  Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 54 - 54, Japanese


• 1P-191 The effect of sea-salts on the cell-growth and photosynthesis in a remarkable carbon feedstock Spirulina

  Aikawa Shimpei, Akimoto Seiji, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 65 - 65, Japanese


• 1P-184 Development of novel yeast cell surface display system for consolidated bioethanol production from lignocellulosic biomass

  Inokuma Kentaro, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 63 - 63, Japanese


• 2P-148 Sugar concentration in liquid hydrolysate of dilute acid pretreated rice straw by nanofiltration

  Sasaki Kengo, Hasunuma Tomohisa, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2013, 日本生物工学会大会講演要旨集, 65, 141 - 141, Japanese


• 統合バイオプロセスによる環境調和型セルロース系エタノール生産に資する前処理技術の開発

  崎濱由梨, 俣野結城, 蓮沼誠久, 蓮沼誠久, 近藤昭彦, 近藤昭彦

  2013, 日本生物工学会大会講演要旨集, 65th


• 発酵阻害物存在下におけるキシロースからのエタノール発酵向上酵母の構築

  崎濱由梨, 蓮沼誠久, 近藤昭彦

  2013, 日本農芸化学会大会講演要旨集(Web), 2013


• 神戸大学大学院工学研究科応用化学専攻 生物化学工学研究室

  近藤 昭彦, 荻野 千秋, 田中 勉, 蓮沼 誠久, 石井 純, 原 清敬, 山田 亮祐, 岡井 直子, 工藤 基徳, 佐々木 建吾, 川口 秀夫, 柘植 陽太

  05 Nov. 2012, 化学工学 = Chemical engineering, 76(11) (11), 722 - 722, Japanese


• Cyanobacterium Synechocystis sp. PCC6803 におけるO2存性オルタナティブ・エレクトロン・フロー (AEF)の生理的役割

  林 良祐, 清水 聡子, 山本 宏, 藍川 晋平, 蓮沼 誠久, 秋本 誠志, 近藤 昭彦, 三宅 親弘

  Mar. 2012, 第53回日本植物生理学会, Japanese


• 4Ip22 Saccharification and fermentation of biomass by the ionic liquid pretreatment with the engineered xylose fermentable yeast

  Ogura Kazuma, Nakashima Nanami, Yamada Ryousuke, Hasunuma Tomohisa, Kamiya Noriho, Ishida Nobuhiro, Saito Satosi, Ogino Chiaki, Kondo Akihiko

  日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 248 - 248, Japanese


• 2Gp16 Metabolome analysis of filamentous fungus Rhizopus oryzae showing different behaviors of lipase secretion

  Yoshida Ayumi, Hama Shinji, Hasunuma Tomohisa, Yamamoto Hiroyuki, Kondo Akihiko

  日本生物工学会, 2012, 日本生物工学会大会講演要旨集, 64, 65 - 65, Japanese


• 有用希少イノシトールのバイオアベイラビリティーとバイオコンバージョン生産

  吉田 健一, 蓮沼 誠久

  日本生物工学会, Oct. 2011, 生物工学会誌, 89(10) (10), 585 - 588, Japanese

  Introduction scientific journal


• ＣＢＰ用スーパー酵母を用いたバイオエタノール生産技術の開発

  HASUNUMA TOMOHISA, OGINO CHIAKI, KONDO AKIHIKO

  Oct. 2011, ブレインテクノニュース, 144, 17 - 22, Japanese

  Introduction scientific journal


• バイオマスからの効率的なバイオ燃料の生産技術 (特集 本格化するバイオマスエネルギー開発)

  蓮沼 誠久, 近藤 昭彦

  化学工業社, Sep. 2011, ケミカルエンジニヤリング, 56(9) (9), 661 - 668, Japanese


• バイオマスからの効率的なバイオ燃料の生産技術

  HASUNUMA TOMOHISA, KONDO AKIHIKO

  Sep. 2011, ケミカルエンジニヤリング, 56(9) (9), 5 - 12, Japanese

  Introduction scientific journal


• 11 シアノバクテリアでのオルタナティブ・エレクトロン・フロー(AEF)評価系の確立(関西支部講演会,2010年度各支部会講演要旨)

  進藤 沙織, 林 吉祐, 真野 陽人, 杉本 敏男, 近藤 昭彦, 藍川 晋平, 蓮沼 誠久, 秋本 誠志, 三宅 親弘

  一般社団法人日本土壌肥料学会, 08 Aug. 2011, 日本土壌肥料学会講演要旨集, (57) (57), 329 - 329, Japanese


• 微細藻類によるバイオリファイナリー

  HASUNUMA TOMOHISA, 藍川 晋平, 和泉 自泰, KONDO AKIHIKO

  日本生物工学会, Apr. 2011, 生物工学会誌, 89(4) (4), 181 - 183, Japanese

  Introduction scientific journal


• 1Kp08 Flux balance analysis (MFA) of xylose-fermenting Saccharomyces cerevisiae strains

  Kato Hiroko, Nagata Kento, Yamada Ryosuke, Hasunuma Tomohisa, Matsuda Fumio, Kondo Akihiko

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 68 - 68, Japanese


• 1Kp10 Improvement of xylose fermenting recombinant Saccharomyces cerevisiae by controlling intracellular redox balances

  Suga Hiroyuki, Hasunuma Tomohisa, Matsuda Fumio, Kondou Akihiko

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 69 - 69, Japanese


• 1Ap02 Deletion of PHO13 gene in a recombinant Saccharomyces cereviseae strain confers tolerance to fermentation inhibitors

  Fujitomi Keisuke, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 21 - 21, Japanese


• 1Ap20 High density CO_2 fixation cycle by Euglena gracilis for biofuel production

  Hayashi Masahiro, Matsuda Fumio, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 25 - 25, Japanese


• 2Ip14 High efficient ethanol production from hydrothermally-processed rice straw using cellulase-displaying yeast with a rotary fermenter

  Matano Yuki, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 180 - 180, Japanese


• 2Ka04 Reduction of furan derivatives by NADH-dependent Adh1 improves ethanol fermentation using xylose as sole carbon source with Saccharomyces cerevisiae harboring XR-XDH pathway

  Ishii Jun, Yoshimura Kazuya, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 187 - 187, Japanese


• 2Ap03 Repeated-batch fermentation of lignocellulosic hydrolysate to ethanol using a hybrid Saccharomyces cerevisiae strain metabolically engineered for tolerance to fermentation inhibitors

  Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2011, 日本生物工学会大会講演要旨集, 63, 113 - 113, Japanese


• バイオマスからのバイオ燃料生産に向けた戦略

  KONDO Akihiko, OGINO Chiaki, HASUNUMA Tomohisa, TANAKA Tsutomu, NAKASHIMA Kazunori

  シーエムシー出版, Nov. 2010, バイオインダストリー, 11, 6-12(11) (11), 6 - 12, Japanese

  Introduction scientific journal


• 次世代燃料・化成品原料製造に向けたバイオリファイナリー戦略

  KONDO Akihiko, OGINO Chiaki, HASUNUMA Tomohisa, TANAKA Tsutomu, NAKASHIMA Kazunori

  日本生物工学会, Jul. 2010, 生物工学, 88(7), 333-335(7) (7), 333 - 335, Japanese

  Introduction scientific journal


• 2-IV-14 D-アラビノースは酵母エリスロアスコルビン酸の内在性前駆体ではない(一般演題,日本ビタミン学会第62回大会発表要旨)

  尼子 克己, 森村 健一, 蓮沼 誠久, 近藤 昭彦, 合田 清

  日本ビタミン学会, 25 Apr. 2010, ビタミン, 84(4) (4), 206 - 206, Japanese


• 3P-1139 Development of a comprehensive metabolic profiling technique for system biology analysis of microalgae

  IZUMI Yoshihiro, AIKAWA Shimpei, MATSUDA Fumio, HASUNUMA Tomohisa, KONDO Akihiko

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 92 - 92, Japanese


• 3P-1119 Efficient ethanol production from xylose in the presence of fermentation inhibitors

  SANDA Tomoya, YAMADA Ryosuke, HASUNUMA Tomohisa, KONDO Akihiko

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 87 - 87, Japanese


• 3P-1134 Metabolic analysis of xylose fermentation in recombinant yeast

  MATSUDA Fumio, KATO Hiroko, HASUNUMA Tomohisa, KONDO Akihiko

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 91 - 91, Japanese


• 3S-Ba04 Development of bio-fuel production system using microalgae

  KONDO Akihiko, HASUNUMA Tomohisa

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 202 - 202, Japanese


• 2P-2062 Effect of light intensity on glycogen production and growth rate of cyanobacteria Spirulina (Arthrospira) platensis

  AIKAWA Shimpei, IZUMI Yoshihiro, MATSUDA Fumio, HASUNUMA Tomohisa, KONDO Akihiko

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 122 - 122, Japanese


• 2P-2051 Efficient fermentation of xylose to ethanol in the presence of furan compound by recombinant Saccharomyces cerevisiae expressing Alcohol dehydrogenase

  YOSHIMURA Kazuya, ISHII Jun, HASUNUMA Tomohisa, KONDO Akihiko

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 119 - 119, Japanese


• 3P-1011 Construction of the recombinant xylose fermentation yeast with high β-xylosidase activity from OC-2 triple marker strain as a platform

  Saitoh Satoshi, Tanaka Tsutomu, Hasunuma Tomohisa, Kondo Akihiko

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 60 - 60, Japanese


• 2S-Dp02 Production and bioavailability of rare inositols

  YOSHIDA Ken-ichi, HASUNUMA Tomohisa

  日本生物工学会, 2010, 日本生物工学会大会講演要旨集, 22, 229 - 229, Japanese


• Applications of yeast cell-surface display in bio-refinery

  Akihiko Kondo, Tsutomu Tanaka, Tomohisa Hasunuma, Chiaki Ogino

  2010, Recent Patents on Biotechnology, 4(3) (3), 226 - 234, English

  [Refereed]

  Introduction scientific journal


• SB-O5 Dynamic metabolic profiling in plant leaves using in vivo stable isotope labeling(Section XI Systems Biotechnology/Metabolic Engineering)

  Hasunuma Tomohisa, Kondo Akihiko, Fukusaki Eiichiro

  The Society for Biotechnology, Japan, Nov. 2009, Journal of bioscience and bioengineering, 108(1) (1), S167, English


• Dynamic metabolic profiling in plant leaves using in vivo stable isotope labeling

  Tomohisa Hasunuma, Akihiko Kondo, Eiichiro Fukusaki

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108, S167 - S167, English

  Summary international conference


• Bioethaol fermentation from mixed sugar by the recombinant yeast with xyloseisomerase pathway

  Tanino Takanori, Hotta Atsushi, Ito Tomonori, Ishii Jun, Yamada Ryosuke, Hasunuma Tomohisa, Ogino Chiaki, Ohmura Naoto, Ohshima Takayuki, Kondo Akihiko

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(1) (1), S53, English

  [Refereed]

  Summary international conference


• Bioethanol production from mixed sugars using sugar uptake ability enhanced yeast strain by overexpression of transporters

  Hotta Atsushi, Tanino Takanori, Ito Tomonori, Hasunuma Tomohisa, Ogino Chiaki, Kondo Akihiko, Ohmura Naoto

  Nov. 2009, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(1) (1), S53, English

  [Refereed]

  Summary international conference


• バイオマスからの化学品生産を目指したバイオプロセスの開発

  HASUNUMA Tomohisa, OKANO Kenji, TATENO Toshihiro, KONDO Akihiko

  化学工業社, Mar. 2009, ケミカルエンジニヤリング, Vol 54. No. 3, pp. 24-30(3) (3), 192 - 198, Japanese

  Introduction scientific journal


• 3S15a04 Bioproduction through synthetic bioengineering

  KONDO Akihiko, FUKUSAKI Eiichiro, HASUNUMA Tomohisa

  日本生物工学会, 2009, 日本生物工学会大会講演要旨集, 21, 292 - 292, Japanese


• CE/MSを用いた一次代謝物の安定同位体標識率モニタリング法の開発

  原田和生, 大山陽子, 蓮沼誠久, 宮澤真一, 三宅親弘, 小林昭雄, 福崎英一郎

  2008, 日本農芸化学会大会講演要旨集, 2008


• ¹³CO₂エンリッチメントによる代謝ターンオーバー解析

  蓮沼誠久, 原田和生, 大山陽子, 宮澤真一, 福崎英一郎, 三宅親弘

  2008, 日本植物生理学会年会要旨集, 49th


• ¹³CO₂を用いた同位体標識による葉内代謝物質のターンオーバー解析

  蓮沼誠久, 原田和生, 三宅親弘, 福崎英一郎

  2008, 生化学


• Dynamic metabolic profiling using in vivo stable isotope labeling

  蓮沼 誠久, 原田 和生, 三宅 親弘, 福崎 英一郎

  生体試料に含まれる代謝産物の量的情報をMSやNMRを利用して網羅的に解析する代謝プロファイリング技術は，微量な中間代謝物質を含む多様な化合物の一斉分析を可能にしたが，ここで得られる情報は代謝物質を抽出した時点での蓄積量のスナップショットであった．生体内で代謝物質が動的定常状態にある時，総量は変わらずに一定の同じ速度で分解と合成が行われて入れ替わっており，新規合成される物質の存在比率を知るためには安定同位体を用いたin vivo標識が必須である．代謝プロファイリングと安定同位体標識法の組合せを利用した「動的代謝プロファイリング」は，代謝産物の量的変動を網羅的に観測することを可能にする．植物の環境変化やストレスに対する応答はシステマティックな代謝変動に基づくことが予想され，「動的代謝プロファイリング」はこうした複雑な応答機構の解明に寄与することが期待できる．Metabolic profiling technique has enabled to analyze the level of various metabolites including minor intermediates by using MS or NMR simultaneously, but the information available from the technique was snapshot in vivo at the time of sampling metabolites. In the dynamic steady state, metabolites are replaced with newly-synthesized compounds at a constant rate and the total amount of metabolites remains unchanged. Therefore, in vivo labeling with stable isotopes is necessary for the determination of turnover rates of metabolites. Dynamic metabolic profiling formed by the combination of metabolic profiling and stable isotope labeling technique enables to determine changes in metabolic flux exhaustively. In plants, responses to stress or environmental changes would be based on systematic variations in metabolism. The dynamic metabolic profiling would contribute to elucidate the complicated response mechanism.光合成研究法. 北海道大学低温科学研究所, 日本光合成研究会共編

  北海道大学低温科学研究所 = Institute of Low Temperature Science, Hokkaido University, 2008, Low temperature science, 67, 169 - 174, Japanese


• Expression of Aspergillus niger pectin methylesterase in transgenic tobacco (Nicotiana tabacum) leads to alterations on the level of methanol and a dwarf phenotype

  Hasunuma Tomohisa, Fukusaki Eiichiro, Okazawa Atsushi, Kajiyama Shinichiro, Kobayashi Akio

  公益社団法人日本生物工学会, 25 Aug. 2003, 日本生物工学会大会講演要旨集, 15, 184 - 184, Japanese


• In vitro selection of DNA aptamers that bind specifically to tubulin

  Hasunuma Tomohisa, Fukusaki Ei-ichiro, Okazawa Atsushi, Kajiyama Shin-ichiro, Kobayashi Akio

  公益社団法人日本生物工学会, 10 Jul. 2000, 日本生物工学会大会講演要旨集, 12, 148 - 148, Japanese

• Machine Learning and Big Data-enabled Biotechnology

  Tanaka, K., Vavricka, CJ, Hasunuma, T.

  Joint work, Metabolomics: Big Data Approaches, Wiley-VCH, Mar. 2026


• 応用微生物学 第4版

  蓮沼誠久

  Joint work, 第10章 循環型未来社会への取組み -6.バイオ燃料, 文永堂出版, Mar. 2025


• テクノロジー・ロードマップ 2025-2034 全産業編

  蓮沼誠久

  Joint work, 第15章 材料/製造, 5.スマートセル・バイオものづくり, 日経BP, Nov. 2024


• カロテノイドの科学 -基礎, 研究の新展開, 生理活性-

  蓮沼 誠久

  Joint work, 第11章 微生物によるカロテノイド高生産とメタボローム解析の応用, Mar. 2024


• バイオマスの科学と技術 -未来を創るエネルギー-

  蓮沼 誠久

  Joint work, 第8章 バイオマスの生物化学的変換 ５節 代謝と遺伝子工学の利用, コロナ社, 2024


• テクノロジー・ロードマップ 2024-2033 全産業編

  蓮沼誠久

  Joint work, 第14章 材料/製造, 6.スマートセルインダストリー, 日経BP, Nov. 2023


• CO2の有効利用技術の開発

  加藤悠一, 田中謙也, 蓮沼誠久

  Joint work, 第5章 第14節 シアノバクテリアを利用した二酸化炭素からの有用物質生産技術, 技術情報協会, Jul. 2023


• 微生物を活用した有用物質の製造技術

  秀瀬涼太, 蓮沼誠久

  Joint work, 第5章 CO2からの有用物質生産 -2. 水素酸化細菌によるCO2吸収-, 微生物を活用した有用物質の製造技術, シーエムシー出版, May 2023


• 微生物を活用した有用物質の製造技術

  蓮沼誠久

  Joint work, 第2章 高度なバイオものづくり用微生物を開発するための要素技術 -6. 微生物物質生産に資するオミクス技術-, シーエムシー出版, May 2023


• 農業・食料生産分野におけるバイオマス利用工学 -循環型社会のための生物資源利用-

  蓮沼誠久

  Joint work, I編 生物資源利用の基礎：3. 物理・化学的変換技術の理論「3.3 バイオリファイナリー」, 119-128, Ⅱ編 生物資源の応用技術：2. エネルギー利用技術「2.4 バイオリファイナリー産物」, 229-237, Apr. 2023


• Handbook of Biorefinery Research and Technology

  Kahar, P., Guirimand, G., Hasunuma, T.

  Ethanol Production by Recombinant CBP Yeasts, Springer, 2023


• クリーンエネルギーの技術と市場 2022

  猪熊健太郎, 蓮沼誠久

  Joint work, 第9章 バイオマス燃料生産の技術動向, シーエムシー出版, Feb. 2022


• 醸造の辞典

  蓮沼誠久

  第2章 醸造の科学, 最近の育種技術, 朝倉書店, Jun. 2021


• バイオリアクターのスケールアップと物質生産事例集

  蓮沼誠久

  第11章 スマートセルの動向と効率的創製に向けたAI活用, 第1節 国内におけるスマートセルの動向, 技術情報協会, Apr. 2021


• メタボロミクス 実践ガイド

  蓮沼誠久

  応用・展望編 Ⅰ. 応用研究 代謝工学分野へのメタボロミクスの応用, 羊土社, Apr. 2021


• Carotenoids: Biosynthetic and Biofunctional Approaches

  Yuichi Kato, Tomohisa Hasunuma

  Metabolic Engineering for Carotenoid Production Using Eukaryotic Microalgae and Prokaryotic Cyanobacteria, Springer, 2021


• 脱石油に向けたCO2資源化技術―化学・生物プロセスを中心に―

  蓮沼誠久

  Joint work, 第Ⅲ編 生物プロセス, 第2章 ラン藻によるバイオコハク酸生産, シーエムシー, Jul. 2020


• 骨格筋研究を核とした筋スマート社会

  西村 勇哉, 蓮沼 誠久

  Joint work, 第3章 7. 組織内の分子を網羅的に測る技術, シーエムシー・リサーチ, Jun. 2019


• 第9章 油脂酵母による油脂発酵生産性改善へ向けた技術開発, スマートセルインダストリー -微生物細胞を用いた物質生産の展望-, 第3編 産業応用へのアプローチ

  高久 洋暁, 荒木 秀雄, 小笠原 渉, 田代 康介, 蓮沼 誠久, 油谷 幸代, 矢追 克郎

  Joint work, シーエムシー出版, Jun. 2018, Japanese

  Scholarly book


• スマートセル設計に資するメタボローム解析, スマートセルインダストリー -微生物細胞を用いた物質生産の展望-, 第1編 ハイスループット合成・分析・評価技術, 第3章 オミクス解析技術

  蓮沼 誠久

  Joint work, シーエムシー出版, Jun. 2018, Japanese

  Scholarly book


• スマートセルインダストリー –微生物細胞を用いた物質生産の展望–（第1編 ハイスループット合成・分析・評価技術，第2章 ハイスループット微生物構築・評価技術，第1節 「微生物を用いた物質生産とハイスループット微生物構築技術」）

  ISHII JUN, 西 晶子, 北野 美保, 中村 朋美, SHOUJI SHINICHIROU, HIDESE RYOTA, HASUNUMA TOMOHISA, KONDO AKIHIKO

  Joint work, シーエムシー出版, Jun. 2018, Japanese

  Scholarly book


• スマートセルインダストリー, 総論 -微生物細胞を用いた物質生産の展望-

  HASUNUMA TOMOHISA, 田村 具博, KONDO AKIHIKO

  Joint work, シーエムシー出版, Jun. 2018, Japanese

  Scholarly book


• Emerging Areas in Bioengineering：Whole cell biocatalysts using enzymes displayed on yeast cell surface

  Kentaro Inokuma, Tomohisa Hasunuma, Akihiko Kondo

  Joint work, Wiley-VCH, Feb. 2018, English

  Scholarly book


• Industrial Biotechnoligy : Products and Processes, Chapter 5, Production of fuels and chemicals from biomass by integrated bioprocesses

  HASUNUMA Tomohisa, KONDO Akihiko

  Joint work, Wley, Nov. 2016, English

  Scholarly book


• 応用微生物学，第10章 低炭素化社会への取組み，バイオ燃料

  蓮沼 誠久

  Joint work, 文永堂出版, Aug. 2016, Japanese

  Scholarly book


• Bioprocessing of Renewable Resources to Commodity Bioproducts, Chapter 8, Ethanol production from yeasts

  HASUNUMA Tomohisa, YAMADA Ryosuke, KONDO Akihiko

  Joint work, Wiley, Apr. 2014, English

  Scholarly book


• 化学便覧 応用化学編

  KONDO AKIHIKO, OGINO CHIAKI, HASUNUMA TOMOHISA, ISHII JUN, HARA KIYOTAKA, OKAI NAOKO, 山田 亮祐

  Joint work, 丸善出版, Jan. 2014, Japanese

  Scholarly book


• 次世代パワートレイン開発と燃料技術

  HASUNUMA TOMOHISA, KONDO AKIHIKO

  Joint work, シーエムシー出版, Nov. 2013, Japanese

  Scholarly book


• リサイクルバイオテクノロジーの最前線, 第I編 第1章, 8 微細藻類・シアノバクテリアからのバイオエタノール生産

  HASUNUMA TOMOHISA

  Joint work, シーエムシー出版, May 2013, Japanese

  Scholarly book


• リサイクルバイオテクノロジーの最前線, 第I編 第1章, 1 未来型CBP法によるバイオエタノール生産技術の展開

  HASUNUMA TOMOHISA, OGINO CHIAKI, KONDO AKIHIKO

  Joint work, シーエムシー出版, May 2013, Japanese

  Scholarly book


• ひらく、ひらく「バイオの世界」

  崎濱 由梨, HASUNUMA TOMOHISA, KONDO AKIHIKO

  Joint work, 化学同人, Oct. 2012, Japanese

  Scholarly book


• 微細藻類によるエネルギー生産と事業展望

  HASUNUMA TOMOHISA, KONDO AKIHIKO

  Joint work, シーエムシー出版, Jul. 2012, Japanese

  Scholarly book


• 藻類ハンドブック

  HASUNUMA TOMOHISA, KONDO AKIHIKO

  Joint work, エヌ・ティー・エス, Jul. 2012, Japanese

  Scholarly book


• 合成生物工学の隆起

  HASUNUMA TOMOHISA, KONDO AKIHIKO

  Joint work, シーエムシー出版, Apr. 2012, Japanese

  Scholarly book


• 微生物機能学

  HASUNUMA TOMOHISA, KONDO AKIHIKO

  Joint work, 三共出版, Mar. 2012, Japanese

  Scholarly book


• バイオマス分解酵素研究の最前線, 第7編, 第28章 セルラーゼ細胞表層提示酵母を用いたバイオマス変換

  山田 亮祐, 蓮沼 誠久, 近藤 昭彦

  Joint work, シーエムシー出版, Mar. 2012, Japanese

  Scholarly book


• バイオマス分解酵素研究の最前線, 序章, 2 バイオマス分解酵素研究の新たな展開

  蓮沼 誠久, 近藤 昭彦

  Joint work, シーエムシー出版, Mar. 2012, Japanese

  Scholarly book


• System Metabolic Engineering

  HASUNUMA Tomohisa, MATSUDA Fumio, KONDO Akihiko

  Joint work, Springer, 2012, English

  Scholarly book


• エコバイオリファイナリー

  KONDO Akihiko, OGINO Chiaki, HASUNUMA Tomohisa, TANAKA Tsutomu, NAKASHIMA Kazunori

  Joint work, シーエムシー出版, Dec. 2010, Japanese

  Scholarly book


• グリーンバイオケミストリーの最前線

  OGINO Chiaki, HASUNUMA Tomohisa, KONDO Akihiko

  Joint work, シーエムシー出版, Apr. 2010, Japanese

  Scholarly book


• Methods in Molecular Biology

  蓮沼 誠久, 近藤 昭彦, 三宅 親弘

  Joint work, Humana Press, Feb. 2010, English

  Scholarly book


• 次世代バイオエタノール生産の技術革新と事業展開, 第7章, ４ アーミング酵母を用いた統合プロセスによるバイオエタノール生産

  蓮沼 誠久, 山田 亮祐, 近藤 昭彦

  Joint work, フロンティア出版, Jan. 2010, Japanese

  Scholarly book


• 次世代バイオエタノール生産の技術革新と事業展開, 第10章, 7 アーミング技術を応用した新規セルロース分解システムの構築

  山田 亮祐, 蓮沼 誠久, 近藤 昭彦

  Joint work, フロンティア出版, Jan. 2010, Japanese

  Scholarly book


• 第二世代バイオ燃料の開発と応用展開

  近藤 昭彦, 福田 秀樹, 荻野 千秋, 田中 勉, 蓮沼 誠久, 原田 和生, 福崎 英一郎

  Joint work, シーエムシー出版, Apr. 2009, Japanese

  Scholarly book


• 水環境の今と未来 藻類と植物のできること

  HASUNMA Tomohisa, KONDO Akihiko

  Joint work, 生物研究社, Mar. 2009, Japanese

  General book


• 光合成研究法

  HASUNMA Tomohisa, HARADA Kazuo, MIYAKE Chikahiro, FUKUSAKI Ei-ichiro

  Joint work, 北海道大学低温科学研究所・日本光合成研究会共編, Mar. 2009, Japanese

  Scholarly book

• ポストコロナ時代の産学連携について考える

  蓮沼誠久

  レーザー学会学術講演会第41回年次大会シンポジウム, Jan. 2021, Japanese


• バイオプロセスにおける膜利用の現状と将来展望

  蓮沼誠久

  第一回先端膜工学研究推進機構特定テーマフォーラム～医薬・バイオプロセスにおける膜利用の現状と将来展望～, Dec. 2020, Japanese


• Metabolomics-based engineering biology for microbial bio-production with sustainability

  Tomohisa Hasunuma

  2nd ASBA Webinar, Dec. 2020, English


• ハイスループットプラットフォームの開発による微生物スマートセルの構築

  蓮沼誠久

  CBI学会2020年大会, Oct. 2020, Japanese


• 微細藻類を利用した物質生産プロセスへのLEDの応用

  蓮沼誠久, 加藤悠一

  JPC関西特別報告会, Oct. 2020, Japanese


• メタボローム解析の自動化に向けた挑戦

  蓮沼誠久

  Laboratory Automation勉強会, Aug. 2020, Japanese


• バイオ×デジタルの技術融合による有用微生物「スマートセル」開発への挑戦

  蓮沼誠久

  質量分析インフォマティクス研究会 第５回ワークショップ, Aug. 2020, Japanese

  [Invited]


• スマートセルインダストリーに資するハイスループット微生物構築・評価技術の開発

  蓮沼 誠久

  日本農芸化学会2020年度大会, Japanese


• スマートセル開発に資するハイスループット微生物構築・評価技術の概要

  蓮沼 誠久

  NEDOフェスタin関西 2019, Dec. 2019, Japanese, Domestic conference

  [Invited]

  Public discourse


• 酵母Saccharomyces cerevisiaeの鉄代謝改変に基づくキシロースからの1,2,4-ブタントリオール発酵生産

  湯川貴弘, 番場崇弘, 蓮沼誠久, 近藤昭彦

  日本農芸化学会関西支部 支部例会（第511回講演会）, Dec. 2019, Japanese, Domestic conference

  Oral presentation


• スマートセルインダストリーに資するメタボローム解析技術の開発と応用

  蓮沼 誠久

  日本農芸化学会関西支部 支部例会（第511回講演会）, Dec. 2019, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• 藍藻の動的メタボローム解析技術の開発と応用

  蓮沼 誠久

  藍藻の分子生物学2019, Nov. 2019, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• バイオ×デジタルによるスマートセル開発への挑戦

  蓮沼 誠久

  Bio Japan 2019, Oct. 2019, Japanese, Domestic conference

  [Invited]

  Nominated symposium


• Metabolomics-based engineering for smart cell development

  Tomohisa Hasunuma

  Metabolic Engineering Summit 2019, Oct. 2019, English, International conference

  Oral presentation


• Metabolomics-based engineering biology for bio-production

  Tomohisa Hasunuma

  2019 Asian Synthetic Biology Association (ASBA) Meeting, Oct. 2019, English, International conference

  Oral presentation


• Re-design, synthesis, and bio-analysis of sulfosialic acids as covalent inhibitors of neuraminidase

  ヴァヴリッカ クリストファー, 松本達磨, 荒木通啓, 蓮沼誠久, 泉実, 清田洋正

  第61回天然有機化合物討論会, Sep. 2019, English, Domestic conference

  Poster presentation


• スマートセル開発に資するハイスループット微生物構築・評価技術の概要

  蓮沼 誠久

  2019年度NEDOスマートセルプロジェクト技術セミナー, Sep. 2019, Japanese, Domestic conference

  Invited oral presentation


• 窒素源存在下で油脂を高蓄積する海洋性クラミドモナス変異株の選択的育種

  OYAMA TOMOKI, KATO YUICHI, 佐藤 勝也, 大野 豊, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference

  Oral presentation


• 食農産業における LED の利活用技術の開発

  藤川 康夫, 鶴本 智大, 泉田 智史, 三島 俊介, 岡澤 敦司, KATO YUICHI, HASUNUMA TOMOHISA

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference

  Oral presentation


• 細胞表層提示システムに適した宿主酵母の開発

  INOKUMA KENTARO, KITADA YUKI, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference

  Oral presentation


• 海洋性クラミドモナス油脂高蓄積変異株における油脂・カロテノイド増産メカニズムの解明

  KATO YUICHI, OYAMA TOMOKI, 佐藤 勝也, 大野 豊, 張 嘉修, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学, Domestic conference

  Oral presentation


• Pichia pastorisにおいて分泌シグナル配列の１アミノ酸置換はタンパク質分泌生産量を劇的に増大させる

  ITO YOICHIRO, 石上 美佐, 橋場 倫子, NAKAMURA YASUYUKI, HASUNUMA TOMOHISA, ISHII JUN, KONDO AKIHIKO

  第71回日本生物工学会大会, Sep. 2019, Japanese, 岡山大学 津島キャンパス, Domestic conference

  Oral presentation


• Smart Cell Development Based on High Throughput Technology, Advanced Analysis and Computational Science for Bio-production in Microorganisms

  Tomohisa Hasunuma

  The 14th Asian Congress on Biotechnology (ACB2019), Jul. 2019, English, International conference

  [Invited]

  Invited oral presentation


• 酵母 Scheffersomyces stipitisを用いたバイオマス資源からの有用芳香族化合物生産プロセスの開発

  小林 優真, 猪熊 健太郎, 蓮沼 誠久, 近藤 昭彦

  生物工学若手研究者の集い 夏のセミナー2019, Jul. 2019, Japanese, Domestic conference

  Poster presentation


• Metabolomics-based engineering biology of microorganisms

  Tomohisa Hasunuma

  Metabolomics 2019, Jun. 2019, English, International conference

  Oral presentation


• スマートセル創出プラットフォーム構築へ向けた挑戦

  HASUNUMA TOMOHISA

  第6回SBJシンポジウム, May 2019, Japanese, 千里ライフサイエンスセンター, Domestic conference

  Oral presentation


• 緑色LEDを用いた微細藻類バイオマス増産技術の開発

  加藤 悠一, 藤川 康夫, 三島 俊介, 泉田 智史, 蓮沼 誠久, 近藤 昭彦

  日本農芸化学会2019年度大会, Mar. 2019, Japanese, 東京農業大学 世田谷キャンパス, Domestic conference

  Poster presentation


• 4-アミノフェニルアラニン発酵における組換え大腸菌の代 謝改変

  川口 秀夫, 若井 桂子, 蓮沼 誠久, 桝尾 俊介, 高谷 直樹, 近藤 昭彦

  日本農芸化学会2019年度大会, Mar. 2019, Japanese, 東京農業大学 世田谷キャンパス, Domestic conference

  Poster presentation


• スマートセル創出プラットフォームの構築と実証に向けて

  蓮沼 誠久

  関西スマートセルフォーラム2018 第3回セミナー, Jan. 2019, Japanese, 大阪中之島センター, Domestic conference

  Oral presentation


• Platform Technologies for Development of Smart Cell

  TOMOHISA Hasunuma

  Asian Synthetic Biology Association 2018, Nov. 2018, English, Hyatt Regency Jeju, International conference

  Oral presentation


• Development of "smart cell" construction platform for next-generation microbial breeding

  TOMOHISA Hasunuma

  IEE CPMT Symposium Japan 2018, Nov. 2018, English, 京都大学, International conference

  [Invited]

  Invited oral presentation


• Construction of a stable, autonomously replicating plasmid vector containing Pichia pastoris centromeric DNA

  NAKAMURA YASUYUKI, Teruyuki Nishi, Risa Noguchi, ITO YOICHIRO, Toru Watanabe, Tozo Nishiyama, Shimpei Aikawa, Tomohisa Hasunuma, ISHII JUN, Yoshihiko Yasohara, Akihiko Kondo

  The Symposium on Biorefinery and Biprocess Topics, 2018 (iBio-N 2018), Nov. 2018, English, Nanjing, China, International conference

  Poster presentation


• 次世代型微生物育種に資するスマートセル創出プラットフォームの開発

  蓮沼 誠久

  BioJapan 2018, Oct. 2018, Japanese, パシフィコ横浜, Domestic conference

  [Invited]

  Invited oral presentation


• M2p3 Non-conventional酵母 Scheffersomyces stipitisを用いたレスベラトロール生産プロセスの開発

  小林優真, 猪熊健太郎, 蓮沼誠久, 近藤昭彦

  日本農芸化学会 関西・中部支部 2019年度合同神戸大会, Sep. 2018, Japanese, Domestic conference

  Oral presentation


• 酵母の代謝解析の最新知見と化粧品原料への応用

  蓮沼 誠久

  第70回日本生物工学会大会 SK-IIランチョンセミナー, Sep. 2018, Japanese, Domestic conference

  Public discourse


• 先端バイオ工学研究センターについて

  蓮沼 誠久

  iBioK第8回合成生物工学シンポジウム, Sep. 2018, Japanese, Domestic conference

  Nominated symposium


• 二次代謝系の動的特性解析における大過剰化合物測定の有無の影響の数理解析

  富永 大輔, 川口 秀夫, 堀 良美, 蓮沼 誠久, 荻野 千秋, 油谷 幸代

  第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 鉄硫黄タンパク質高活性化に基づくキシロースからの1,2,4-ブタントリオール生産技術の開発

  湯川 貴弘, 番場 崇弘, 蓮沼 誠久, 近藤 昭彦

  第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 組換え出芽酵母Saccharomyces cerevisiaeによるキシロースからのグルタチオン生産

  小林 淳平, 佐々木 大介, 番場 崇弘, 蓮沼 誠久, 近藤 昭彦

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 接合型変換を利用したセルラーゼ群表層提示酵母の糖化活性及び発酵阻害物耐性向上

  北田 雄基, 猪熊 健太郎, 蓮沼 誠久, 近藤 昭彦

  第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 酵母Scheffersomyces stipitisを用いたレスベラトロール生産プロセスの開発

  小林 優真, 猪熊 健太郎, 蓮沼 誠久, 近藤 昭彦

  第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 酵母Scheffersomyces stipitisを宿主とした新規細胞表層提示システムの開発

  猪熊 健太郎, 小林 優真, 蓮沼 誠久, 近藤 昭彦

  第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• 遺伝子組換え効率向上に向けたDNAリガーゼIV欠損Pichia pastoris株の開発

  ITO YOICHIRO, 渡邉 徹, 藍川 晋平, 西 輝之, 西山 陶三, NAKAMURA YASUYUKI, HASUNUMA TOMOHISA, 八十原 良彦, ISHII JUN, KONDO AKIHIKO

  第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会, 関西大学 千里山キャンパス, Domestic conference

  Oral presentation


• ラン藻窒素代謝メカニズムの解明と物質生産への応用

  見市 絢香, 蓮沼 誠久, 近藤 昭彦

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• スマートセル創出プラットフォームに資するメタボローム解析技術の開発

  蓮沼 誠久

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Oral presentation


• Pichia pastorisにおけるセントロメアDNA配列を用いた自律複製型プラスミドベクターの開発

  NAKAMURA YASUYUKI, 西 輝之, 野口 理紗, ITO YOICHIRO, 渡邉 徹, 西山 陶三, 藍川 晋平, HASUNUMA TOMOHISA, ISHII JUN, 八十原 良彦, KONDO AKIHIKO

  第70回日本生物工学会大会, Sep. 2018, Japanese, 日本生物工学会, 関西大学 千里山キャンパス, Domestic conference

  Oral presentation


• Temperature dependent succinate biosynthesis concurrent with alteration in central metabolism turnover in synechocystis SP.PCC6803

  TOMOHISA Hasunuma

  The International Symposium on Phototrophic Prokaryotes (ISPP), Aug. 2018, English, the Life Sciences Centre, The University of Buritish Columbia, International conference

  Oral presentation


• 動的メタボロミクスの開発とスマートセルインダストリーへの展開

  蓮沼 誠久

  第13回 アジレントメタボロミクスセミナー, Jul. 2018, Japanese, 慶応義塾大学 三田キャンパス, Domestic conference

  [Invited]

  Invited oral presentation


• スマートセル創出プラットフォームに資するハイスループット微生物構築・評価技術の開発

  蓮沼 誠久

  第20回 新産業技術促進検討会, Jul. 2018, Japanese, TKPガーデンシティPREMIUM神保町 プレミアムボールルーム, Domestic conference

  Oral presentation


• Dynamic Metabolomics of Crabtree-Negative Yeast Kluyveromyces Marxianus

  TOMOHISA Hasunuma

  Metabolic Engineering 12, Jun. 2018, English, The Westin Grand Munich, International conference

  Oral presentation


• Converting Chlamydomonas sp. JSC4 lipids to biodiesel via biocatalysis with Fusarium heterosporum lipase-expressing Aspergillus oryzae whole-cell

  JEROME Amoah, SHIH-HSIN. Ho, SHINJI Hama, AYUMI Yoshida, AKIHITO Nakanishi, TOMOHISA Hasunuma, CHIAKI Ogino, AKIHIKO Kondo

  The 8th International Conference on Algal Biomass, Biofuels and Bioproducts (AlgalBBB 2018), Jun. 2018, English, Motif Seattle, International conference

  Poster presentation


• スマートセルインダストリーの創出に資する微生物育種プラットフォームの開発

  蓮沼 誠久

  ライフサイエンス技術部会・反応分科会講演会講演会「スマートセルインダストリーに関する研究開発動向」, Feb. 2018, Japanese, Domestic conference

  [Invited]

  Public discourse


• Enhanced protein secretion by accumulation of novel effective factors in Pichia pastoris

  ITO YOICHIRO, NAKAMURA YASUYUKI, Shimpei Aikawa, Tomohisa Hasunuma, ISHII JUN, Akihiko Kondo

  The 9th International Symposium of Innovative BioProduction Kobe (iBioK), Feb. 2018, English, Kobe, Japan, International conference

  Poster presentation


• A new autonomous replicating plasmid vector containing Pichia pastoris centromeric DNA

  NAKAMURA YASUYUKI, Teruyuki Nishi, Risa Noguchi, ITO YOICHIRO, Toru Watanabe, Tozo Nishiyama, Shimpei Aikawa, Tomohisa Hasunuma, ISHII JUN, Yuji Okubo, Akihiko Kondo

  The 9th International Symposium of Innovative BioProduction Kobe (iBioK), Feb. 2018, English, Kobe, Japan, International conference

  Poster presentation


• 藻類オイル生産の実用化に向けた代謝メカニズム解析の重要性

  蓮沼 誠久

  シンポジウム「微細藻類を利用したバイオ燃料生産の研究開発動向」, Jan. 2018, Japanese, オフィス東京, Domestic conference

  Oral presentation


• Efficient Xylitol Production from Kraft Pulp by a Cell Surface Engineered Strain ofSaccharomyces cerevisiae

  GUIRIMAND GEORGES, YVES GREGORY, Kentaro Inokuma, Takahiro Bamba, Kengo Sasaki, Chiaki Ogino, Tomohisa Hasunuma, Akihiko Kondo

  5th Asian Conference on Biomass Science, Jan. 2018, English, 日本エネルギー学会, 仙台市, International conference

  Poster presentation


• Engineering Biologyによるバイオリファイナリーの構築とスマートセルインダストリーへの展開

  蓮沼 誠久

  静岡大学グリーン科学研究所 第4回シンポジウム, Nov. 2017, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• 動的メタボロミクスの開発とスマートセルインダストリーへの展開

  蓮沼 誠久

  第11回メタボロームシンポジウム, Nov. 2017, Japanese, ホテル阪急エキスポパーク, Domestic conference

  Oral presentation


• Development of dynamic metabolomics and its application to metabolic engineering

  TOMOHISA Hasunuma

  The 8th Kobe University Brussels European Centre Symposium, Nov. 2017, English, Vrije Universiteit Brussel U-Residence, International conference

  Oral presentation


• スマートセルを創出する合成バイオプラットフォームの開発

  蓮沼 誠久

  BioJapan 2017, Oct. 2017, Japanese, パシフィコ横浜, Domestic conference

  Oral presentation


• 油脂高蓄積クラミドモナス株の選抜育種によるバイオ燃料生産「明暗周期問題」の克服

  KATO YUICHI, 藤原 悠右, 小山 智己, 張 嘉修, Tomohisa Hasunuma, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference

  Oral presentation


• 代謝工学的手法による海洋性ラン藻でのアスタキサンチン生産

  高木 綾湖, 蓮沼 誠久

  第31回カロテノイド研究談話会, Sep. 2017, Japanese, 京都薬科大学, Domestic conference

  Poster presentation


• 代謝工学による黄色天然色素ルテイン高蓄積クラミドモナス株の創出

  阪口 健太, KATO YUICHI, Tomohisa Hasunuma, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference

  Poster presentation


• アスタキサンチン生産能を付与した高増殖性ラン藻のトランスクリプトミクスおよび動的メタボロ ミクス

  高木 綾湖, Tomohisa Hasunuma, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference

  Poster presentation


• Pichia pastorisのタンパク質分泌生産における新規有用因子の獲得とその蓄積による効果の検証

  Yoichiro Ito, Yasuyuki Nakamura, 西 輝之, 藍川 晋平, Tomohisa Hasunuma, Jun Ishii, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 早稲田大学 西早稲田キャンパス, Domestic conference

  Poster presentation


• Pichia pastorisにおけるセントロメア配列を含む自律複製型プラスミドの開発

  Yasuyuki Nakamura, 西 輝之, 野口 理紗, Yoichiro Ito, 渡邉 徹, 西山 陶三, 藍川 晋平, Tomohisa Hasunuma, Jun Ishii, 八十原 良彦, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 早稲田大学 西早稲田キャンパス, Domestic conference

  Poster presentation


• N-アセチルグルコサミン資化性酵母Scheffersomyces stipitisの基質別代謝特性の解析

  Kentaro Inokuma, Tomohisa Hasunuma, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference

  Oral presentation


• Enhanced cell surface engineering of Saccharomyces cerevisiae and optimization of the fermentation process for efficient xylitol production from kraft pulp

  GUIRIMAND GEORGES, YVES GREGORY, Kentaro Inokuma, Takahiro Bamba, Kengo Sasaki, Chiaki Ogino, Tomohisa Hasunuma, Akihiko Kondo

  第69回日本生物工学会大会, Sep. 2017, Japanese, 日本生物工学会, 東京都新宿区, Domestic conference

  Oral presentation


• Challenge to development of “smart” microorganisms based on information analysis and its application to production of highly functional compounds

  TOMOHISA Hasunuma

  第69回日本生物工学会大会 国際シンポジウム, Sep. 2017, English, 早稲田大学 西早稲田キャンパス, International conference

  Oral presentation


• スマートセルを創出する合成バイオプラットフォームの開発と応用への挑戦

  蓮沼 誠久

  合成生物工学シンポジウム, Aug. 2017, Japanese, 神戸大学百年記念館六甲ホール, Domestic conference

  Oral presentation


• Enhancement in central metabolism and carotenoid biosynthesis by photosynthetic astaxanthin production in the recombinant marine cyanobacterium

  TOMOHISA Hasunuma

  The 18th International Symposium on Carotenoids 2017, Jul. 2017, English, The Lucerne Hall, International conference

  Oral presentation


• Cell surface engineering of Saccharomyces cerevisiae for biomass breakdown

  TOMOHISA Hasunuma

  SB7.0 The Seventh International Meeting on Synethetic Biology, Jun. 2017, English, University Cultural Center, International conference

  Oral presentation


• 進化した細胞表層工学によるバイオマス変換プロセスの開発と機能性物質生産への新展開

  蓮沼 誠久

  エネルギー・資源技術部会 バイオマス分科会 講演会「バイオマス資源変換触媒の研究動向」, Apr. 2017, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• 変異チオール酸化酵素による酵母の酸化型グルタチオン 生産

  小林淳平, Sasaki Daisuke, 原 清敬, Hasunuma Tomohisa, KONDO Akihiko

  日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• 代謝工学によるアスタキサンチン産生海洋性ラン藻の 開発と代謝メカニズムの解明

  高木綾湖, Hasunuma Tomohisa, KONDO Akihiko

  日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• バイオ燃料生産に向けた耐塩性緑藻の進化工学的育種と 塩ストレス耐性による油脂生産低下メカニズムの解明

  KATO YUICHI, 賀 詩欣, 張 嘉修, 蓮沼 誠久, 近藤 昭彦

  日本農芸化学会2017年度大会, Mar. 2017, Japanese, 京都女子大学, Domestic conference

  Oral presentation


• バイオ燃料生産に向けた耐塩性緑藻の進化工学的育種と 塩ストレス耐性による油脂生産低下メカニズムの解明

  加藤悠一, 賀 詩欣, 張 嘉修, Hasunuma Tomohisa, KONDO Akihiko

  日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• ゲノム編集による Kluyveromyces marxianus の自在な 遺伝子改変

  南部-西田由美子, 西田敬二, 蓮沼 誠久, 近藤 昭彦

  日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都女子大学, Domestic conference

  Oral presentation


• Saccharomyces cerevisiae による1,2,4-ブタントリオー ル生産技術の開発

  番場崇弘, Hasunuma Tomohisa, KONDO Akihiko

  日本農芸化学2017年度大会, Mar. 2017, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• 統合型バイオエタノール生産プロセスの構築に資する改良型酵 母細胞表層提示用遺伝子カセットの開発

  InokumaKentarou, Hasunuma Tomohisa, KONDO Akihiko

  第12回バイオマス科学会議, Jan. 2017, Japanese, 日本エネルギー学会, 東京都文京区, Domestic conference

  Oral presentation


• バイオリファイナリーの構築に資する微生物細胞工場の創製

  蓮沼 誠久

  第21回関西大学先端科学技術シンポジウム, Jan. 2017, Japanese, 関西大学 千里山キャンパス, Domestic conference

  [Invited]

  Invited oral presentation


• 日本独自の超高速微生物育種プラットフォーム「スマートセル・ファウンドリ」の開発

  蓮沼 誠久

  「植物等の生物を用いた高機能品生産技術の開発」キックオフシンポジウム, Nov. 2016, Japanese, ベイサイドホテル アジュール竹芝, Domestic conference

  Oral presentation


• Development of dynamic metabolic profiling and its application to fuel and chemical production in algae

  TOMOHISA Hasunuma

  The 9th Asia-Pacific Conference on Algal Biotechnology (APCAB), Nov. 2016, English, Century Park Hotel, International conference

  Oral presentation


• 藍藻 Synechocystis sp. PCC 6803 hox遺伝子変異株のコントロールされた培養条件における有機酸 生産

  中島 満晴, 飯嶋 寛子, 白井 智量, 岡本 真美, Hasunuma Tomohisa, KONDO Akihiko, 平井 優美, 小山内 崇

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• 明暗周期下で効率的に油脂を生産するための海洋性緑藻培養技術の開発

  藤原 悠右, 加藤 悠一, 賀 詩欣, Hasunuma Tomohisa, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• 酵母表層への酵素立体的配置技術の開発

  黒野 浩幹, InokumaKentarou, Hasunuma Tomohisa, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• ラン藻スピルリナからの高生産エタノール変換プロセスの開発

  Aikawa Shimpei, InokumaKentarou, Wakai Satoshi, Sasaki Kengo, Hasunuma Tomohisa, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• バイオ医薬生産に向けたCHO細胞培養情報データ解析システムの構築

  許 漢修, 荒木 通啓, Hasunuma Tomohisa, 河野 愛子, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• バイオリファイナリーの構築に資する微生物細胞工場の創生

  蓮沼 誠久

  第118回触媒討論会, Sep. 2016, Japanese, 岩手大学, Domestic conference

  [Invited]

  Invited oral presentation


• N-アセチルグルコサミン資化性酵母Scheffersomyces stipitisの特性解析

  InokumaKentarou, Hasunuma Tomohisa, KONDO Akihiko

  第68回日本生物工学会大会, Sep. 2016, Japanese, 日本生物工学会, 富山市, Domestic conference

  Oral presentation


• Enhanced protein secretion by accumulation of novel effective factors in Pichia pastoris

  Yoichiro Ito, Yasuyuki Nakamura, Aikawa Shimpei, Hasunuma Tomohisa, Ishii Jun, KONDO Akihiko

  The 14th International Congress on Yeasts (ICY2016), Sep. 2016, English, ICY14組織委員会, 淡路市, International conference

  Poster presentation


• Bio-based fuel and chemicalproduction based on dynamic metabolic profiling of cynobacteria and microalgae

  TOMOHISA Hasunuma

  The 4th Asia-Oceania Algae Innovation Summit (AOAIS2016), Sep. 2016, English, East Lake International Conference Center, Wuhan, China, International conference

  Oral presentation


• A new autonomous replicating plasmid vector containing centromere DNA sequence of Pichia pastoris

  NAKAMURA YASUYUKI, Teruyuki Nishi, Risa Noguchi, ITO YOICHIRO, Toru Watanabe, Tozo Nishiyama, Shimpei Aikawa, Tomohisa Hasunuma, ISHII JUN, Yoshihiko Yasohara, Akihiko Kondo

  The 14th International Congress on Yeasts (ICY2016), Sep. 2016, English, Awaji, Japan, International conference

  Poster presentation


• 海洋性ラン藻Synechococcus sp. PCC 7002を用いたアスタキサンチン生産技術の開発

  高木綾湖, HASUNUMA TOMOHISA, KONDO Akihiko

  生物工学若手研究者の集い夏のセミナー2016, Jul. 2016, Japanese, 生物工学会, 府中市, Domestic conference

  Poster presentation


• Development of cell factories of microalgae and cyanobacteria based on metabolic analysis

  Tomohisa Hasunuma

  KMB2016 Annual meeting and international symposium, Jun. 2016, English, International conference

  [Invited]

  Invited oral presentation


• 代謝プロファイリング法の紹介と微生物育種技術への応用

  HASUNUMA TOMOHISA

  環境バイオテクノロジー学会2016年度大会, Jun. 2016, Japanese, サテライトキャンパスひろしま, Domestic conference

  Oral presentation


• Synthetic Promoter Systems for Controlling Saccharomyces Cerevisiae Gene Expression

  Robert Sidney Cox III, Yumiko Nambu, Hasunuma Tomohisa, KONDO Akihiko

  Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conference

  Oral presentation


• Engineering of a Novel Cellulose-Adherent Cellulolytic Saccharomyces Cerevisiae for Cellulosic Biofuel Production

  Zhuo Liu, Inokuma Kentarou, Hasunuma Tomohisa, KONDO Akihiko

  Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conference

  Poster presentation


• Disruption of PHO13 Improves Ethanol Production Via the Xylose Isomerase Pathway

  Takahiro Bamba, Hasunuma Tomohisa, KONDO Akihiko

  Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conference

  Oral presentation


• Direct and High-Productive Conversion from a Cyanobacterium Arthrospira Platensis to Ethanol

  Aikawa Shinpei, Inokuma Kentarou, Hasunuma Tomohisa, KONDO Akihiko

  Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conference

  Oral presentation


• Alternative Inverse Metabolic Engineering Based on Transient Acclimation of Yeast Improves Acid-Containing Xylose Fermentation and Tolerance to Formic and Acetic Acids

  Hasunuma Tomohisa, KONDO Akihiko

  Metabolic Engineering 11, Jun. 2016, English, Metabolic Engineering 11, 淡路市, International conference

  Oral presentation


• 動的代謝プロファイリング技術の開発とバイオリファイナリーへの展開

  蓮沼 誠久

  第340回細胞工学研究会後援会／第220回遺伝子機能解析部門セミナー, Mar. 2016, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• AFM力計測を用いた細胞表層提示酵素の特異的検出とマッピング評価

  Ogino Chiaki, Takenaka Musashi, Inokuma Kentarou, Hasunuma Tomohisa, Kondo Akihiko

  化学工学会第81年会, Mar. 2016, Japanese, Domestic conference

  Oral presentation


• AFM力学計測法を利用した細胞表層空間に提示した酵素検出

  Takenaka Musashi, Ogino Chiaki, Kondo Akihiko, Inokuma Kentarou, Hasunuma Tomohisa, Kobayashi Takuya

  日本化学会第96春季年回, Mar. 2016, Japanese, Domestic conference

  Oral presentation


• Dynamic metabolic analysis of microorganisms for the development of bio-based chemical production

  Tomohisa Hasunuma

  the 7th International Symposium of Innovative BioProduction Kobe (iBioK), Jan. 2016, English, International conference

  Invited oral presentation


• 藻類を利用したバイオエタノール、バイオベース化学品生産

  蓮沼 誠久

  平成27 年度新資源生物変換研究会 シンポジウム, Jan. 2016, Japanese, バイオインダストリー協会, Domestic conference

  Oral presentation


• リグノセルロース系バイオマスの同時糖化発酵によるフェニル乳酸生産

  Kawaguchi hideo, Teramura Hiroshi, Nakamura Satoko, Ogino Chiaki, Hara Kiyotaka, Hasunuma Tomohisa, Rounuma Kenichi, Takatani Naoki, Hirano Wataru, Sazuka Takashi, Kitano Hideki, Kondo Akihiko

  第11回バイオマス科学会議, Jan. 2016, Japanese, International conference

  Oral presentation


• Live cell imaging and membrane protein mapping with atomic force microscope

  Musashi Takenaka, T. Kobayashi, Yusuke Miyachi, Kentarou Inokuma, Jun Ishii, Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015), Dec. 2015, English, International conference

  Oral presentation


• Dynamic metabolic profiling of the marine microalga Chlamydomonas sp. JSC4 and enhancing its oil production

  Hasunuma Tomohisa, Kondo Akihiko

  Pacifichem 2015, Dec. 2015, English, International conference

  Oral presentation


• Development of cell factories of microalgae and cyanobacteria based on metabolic analysis

  Tomohisa Hasunuma

  New Horizon in Biotechnology 2015, Nov. 2015, English, International conference

  [Invited]

  Invited oral presentation


• ラン藻Arthrospira platenisからの高収率エタノール生産プロセスの開発

  Aikawa Shinpei, Inokuma Kentarou, Wakai, Sasaki Kengo, Hasunuma Tomohisa, Kondou Akihiko

  藍藻の分子生物学2015, Nov. 2015, Japanese, Domestic conference

  Oral presentation


• 好機・低濃度グルコース条件下におけるSaccharomyces cerevisiaeの転写解析

  Sakihama Yuri, Hasunuma Tomohisa, Kondou Akihiko

  第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, Japanese, Domestic conference

  Oral presentation


• キチン系基室からのバイオ燃料生産の検討

  Inokuma Kentarou, Hasunuma Tomohisa, Kondou Akihiko

  第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, Japanese, Domestic conference

  Oral presentation


• Saccharomyces cerevisiae の糖代謝における転写制御ネットワークの予測

  Nanbu Yumiko, Sakihama Yuri, Araki, Hasunuma Tomohisa, Kondou Akihiko

  第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, Japanese, Domestic conference

  Oral presentation


• Effect of PHO13 gene disruption on xylose productivity of yeast harboring xylose isomerase gene

  Bamba Takahiro, Hasunuma Tomohisa, Kondou Akihiko

  The 21th Symposium of Yong Asian Biochemical engineer's Communyty, Oct. 2015, English, International conference

  Oral presentation


• Development of microbial cell factories for biorefinery

  Hasunuma Tomohisa, Kondou Akihio

  第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, English, Domestic conference

  Oral presentation


• Conversion of high phospholipid-containing oils to biodiesel using immobilizes Aspergillus oryzae whole cell biocatalyst ezpressing Fusarium heteroporum lipase

  Jerom Amoah, shin-Hsin Ho, Hama Shinji, Yoshida Ayumi, Nakanishi Akihito, Hasunuma Tomohisa, Ogino Chiaki, Kondo Akihiko

  The 21th Symposium of Yong Asian Biochemical engineer's Communyty, Oct. 2015, English, International conference

  Oral presentation


• Combined cell-surface display-and secretion-based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae

  Zuo Liu, Inokuma Kentaro, Hasunuma Tomohisa, Kondo Akihiko

  第67回日本生物工学会大会 国際シンポジウム, Oct. 2015, Japanese, Domestic conference

  Oral presentation


• 藻類のエネルギー生産における物質代謝メカニズムの解析

  蓮沼 誠久

  日本植物学会第79回大会, Sep. 2015, Japanese, 新潟コンベンションセンター, Domestic conference

  Oral presentation


• メタボロームのターンオーバー解析技術（代謝プロファイリング）の開発と物質生産への応用

  蓮沼 誠久

  iBioK第5回 合成生物工学シンポジウム, Aug. 2015, Japanese, Domestic conference

  [Invited]

  Nominated symposium


• マルチオミクス解析に基づく合理的細胞育種技術の開発

  蓮沼 誠久

  HMTセミナー 物質生産におけるメタボロミクスのいま, Jul. 2015, Japanese, Domestic conference

  [Invited]

  Public discourse


• Dynamic metabolic profiling of cyanobacteria for bio-based chemical production

  TOMOHISA Hasunuma

  11th Annual International Conference of the Metabolomics Society, Jun. 2015, English, Hyatt Regency Burlingame, International conference

  Oral presentation


• Biofuel Production from Microalgae and Cyanobacteria Based on Metabolic Profiling

  TOMOHISA Hasunuma

  KMB2015, 42nd Annual Meeting & International Symposium, Jun. 2015, English, HICO, Gyeongju, Korea, International conference

  Oral presentation


• Development of microbial cell factories based on metabolic engineering

  Tomohisa Hasunuma

  2015 KSBB Spring Meeting and International Symposium, Apr. 2015, English, International conference

  [Invited]

  Invited oral presentation


• 産業用酵母のゲノムシャッフリングによる高温及び酸耐性の付与と高効率キシロース発酵

  岩本 遼, 猪熊 健太郎, HASUNUMA TOMOHISA, KONDO AKIHIKO

  日本農芸化学会2015年度大会, Mar. 2015, Japanese, 日本農芸化学会, 岡山市, Domestic conference

  Oral presentation


• アミラーゼ表層提示酵母によるシアノバクテリアArthrospira platensisからの高濃度エタノール生産

  藍川 晋平, 猪熊 健太郎, SASAKI KENGO, HASUNUMA TOMOHISA

  日本農芸化学会2015年度大会, Mar. 2015, Japanese, 日本農芸化学会, 岡山市, Domestic conference

  Oral presentation


• 合成生物工学によるバイオ燃料生産に資する微生物の創製

  蓮沼 誠久

  広島大学バイオマスイブニングセミナー, Dec. 2014, Japanese, Domestic conference

  [Invited]

  Public discourse


• 代謝システム解析に基づく細胞育種とバイオ医薬品生産への応用

  蓮沼 誠久

  第30回バイオロジカルズ（タンパク医薬）製造技術研究会セミナー, Nov. 2014, Japanese, Domestic conference

  [Invited]

  Public discourse


• 代謝システム解析に基づく合理的細胞育種技術の開発

  蓮沼 誠久

  iBioK 第4回合成生物工学シンポジウム, Nov. 2014, Japanese, Domestic conference

  Nominated symposium


• 微細藻類の代謝系解析に基づく次世代バイオ燃料生産への挑戦

  蓮沼 誠久

  バイオマス研究会, Nov. 2014, Japanese, Domestic conference

  [Invited]

  Public discourse


• The development of bioconversion process to bio-ethanol from cyanobacteria Spirulina biomass

  AIKAWA Shinpei, HASUNUMA Tomohisa, KONDO Akihiko

  5th International Symposium on Innovative Bioprocess in Tainan(i-BioT2014), Nov. 2014, English, National Cheng Kung University, Tainan, China, International conference

  Oral presentation


• Development of the novel yeast cell-surface display system for consolidated bioethanol production from biomass resources

  INOKUMA Kentaro, HASUNUMA Tomohisa, KONDO Akihiko

  5th International Symposium on Innovative Bioprocess in Tainan(i-BioT2014), Nov. 2014, English, National Cheng Kung University, Tainan, China, International conference

  Oral presentation


• Development of Dynamic Metabolic Profiling of Cyanobacteria and Microalgae

  HASUNUMA Tomohisa, KONDO Akihiko

  3rd Asia-Oceania Algae Innovation Summit 2014, Nov. 2014, English, AOAIS2014, 済州、韓国, International conference

  Oral presentation


• Development of dynamic metabolic profiling of cyanobacteria and microalgae

  HASUNUMA Tomohisa

  5th International Symposium on Innovative Bioprocess in Tainan(i-BioT2014), Nov. 2014, English, National Cheng Kung University, Tainan, China, International conference

  Oral presentation


• オミクス解析のバイオリファイナリー

  蓮沼 誠久

  アジレント・メタボロミクスデイズ2014, Oct. 2014, Japanese, Domestic conference

  [Invited]

  Public discourse


• Biodiesel production in marine microalgae through metabolic profiling and an innovative salinity-gradient strategy

  Tomohisa Hasunuma

  Korean Marine Biology and Biotechnology International Symposium, Oct. 2014, English, International conference

  [Invited]

  Invited oral presentation


• 代謝系解析に基づくラン藻・微細藻からのバイオ燃料生産への挑戦

  HASUNUMA TOMOHISA

  日本植物学会第78回大会, Sep. 2014, Japanese, 日本植物学会, 川崎市, Domestic conference

  Oral presentation


• 代謝プロファイリングに基づく微生物育種技術の開発と応用

  HASUNUMA TOMOHISA

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• 酵母によるタンパクの高効率細胞表層提示ならびに分泌生産のための新規分泌シグナル配列の検討

  猪熊 健太郎, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• 海産性緑藻 Chlamydomonas sp. JSC4 の細胞組成評価と代謝解析に基づいた油脂高生産系の開発

  中西 昭仁, 賀 詩欣, 張 嘉修, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• 塩ストレス下での海洋性シアノバクテリアの代謝プロファイリング

  西田 篤実, 藍川 晋平, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• バイオ医薬開発に有用なCHO 細胞の改良、培養状態解明につながるデータの構築

  伊賀 朋世, HASUNUMA TOMOHISA, ARAKI MICHIHIRO, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• バイオリファイナリーに向けた微生物の開発と代謝系解析の応用

  HASUNUMA TOMOHISA

  日本植物学会第78回大会ランチョンセミナー, Sep. 2014, Japanese, 島津製作所, 川崎市, Domestic conference

  [Invited]

  Invited oral presentation


• バイオマス糖化液由来成分が大腸菌のフェニル乳酸発酵に与える影響

  KAWAGUCHI HIDEO, 寺村 浩, 中村 聡子, OGINO CHIAKI, HARA KIYOTAKA, HASUNUMA TOMOHISA, 老沼 研一, 高谷 直樹, 平野 恒, 佐塚 隆志, 北野 英己, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• コリネ菌におけるフルフラールの分解

  TSUGE YOTA, 堀 良美, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• キシロース資化性酵母における遺伝子発現プロファイルの経時変化解析

  南部 由美子, 崎濱 由梨, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• アミラーゼ表層提示酵母によるラン藻スピルリナからの高濃度エタノール生産

  藍川 晋平, 猪熊 健太郎, SASAKI KENGO, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• PHO13 遺伝子の欠損がキシロースイソメラーゼ導入酵母のエタノール生産へ与える影響

  番場 崇弘, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• luyveromyces marxianus の好気条件での糖代謝系の解析

  崎濱 由梨, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第66回日本生物工学会大会, Sep. 2014, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• Effect of illumination coupled with nitrogen depletion on biodiesel production of a marine microalga Chlamydomonas sp. JSC4

  HO Shih-Hsin, NAKANISHI Akihito, YE Xiaoting, CHANG Jo-Shu, HARA Kiyotaka, KONDO Akihiko, HASUNUMA Tomohisa

  第66回日本生物工学会大会, Sep. 2014, English, 日本生物工学会, 札幌市, Domestic conference

  Poster presentation


• 色素生産を指標としたCoA代謝経路の評価系

  磯貝 章太, ISHII JUN, HASUNUMA TOMOHISA, KONDO AKIHIKO

  生物工学若手研究者の集い夏のセミナー, Jul. 2014, Japanese, 生物工学会, 神戸市, Domestic conference

  Poster presentation


• キシロースイソメラーゼ導入酵母におけるPHO13遺伝子欠損がエタノール生産へ与える影響

  番場 崇弘, HASUNUMA TOMOHISA, KONDO AKIHIKO

  生物工学若手研究者の集い夏のセミナー, Jul. 2014, Japanese, 生物工学会, 神戸市, Domestic conference

  Poster presentation


• Dynamic Metabolic Profiling of Cyanobacteria Under Conditions of Nitrate Depletion

  HASUNUMA Tomohisa

  Metabolic Engineering X, Jun. 2014, English, Society for Biological Engineering, Vancouver, Canada, International conference

  Oral presentation


• 発酵生産プロセスのための海洋性ラン藻の利用

  藍川 晋平, 西田 篤実, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第16回マリンバイオテクノロジー学会大会, May 2014, Japanese, マリンバイオテクノロジー学会, 津市, Domestic conference

  Poster presentation


• 微細藻類によるバイオ燃料や化学物質製造

  KONDO AKIHIKO, HASUNUMA TOMOHISA

  日本化学会第94春季年会, Mar. 2014, Japanese, 日本化学会, 名古屋市, Domestic conference

  Oral presentation


• 統合型リグノセルロース系エタノール生産プロセスに資するセルラーゼ表層提示酵母の開発

  猪熊 健太郎, 番場 崇弘, HASUNUMA TOMOHISA, KONDO AKIHIKO

  日本農芸化学会2014年度大会, Mar. 2014, Japanese, 日本農芸化学会, 川崎市, Domestic conference

  Oral presentation


• 代謝工学的手法によるグリコーゲン高生産藍藻の創製

  曽根辻 諒彦, 藍川 晋平, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第16回化学工学会学生発表会(堺大会), Mar. 2014, Japanese, 化学工学会, 大阪市, Domestic conference

  Oral presentation


• Development of lipid productive engineering with Chlamydomonas sp. Under marine condition

  HASUNUMA Tomohisa

  Asian Congress on Biotechnology - ACB 2013, Dec. 2013, English, Asian Federation of Biotechnolgy, New Delhi, India, International conference

  Poster presentation


• ラン藻動的代謝プロファイリング技術の開発と応用

  HASUNUMA TOMOHISA

  ラン藻の分子生物学2013, Nov. 2013, Japanese, かずさDNA研究所, 木更津市, Domestic conference

  Oral presentation


• Development of microalgal cell factories based on sysmtems biology approach

  Tomohisa Hasunuma

  1st Korea-Japan Microalgae Symposium, Oct. 2013, English, International conference

  [Invited]

  Invited oral presentation


• Biofuel production from halophilic algae

  Tomohisa Hasunuma

  i-BioB 2013, Oct. 2013, English, International conference

  [Invited]

  Invited oral presentation


• in vivo 13C標識法によるラン藻動的代謝プロファイリング

  蓮沼 誠久

  第28回つくば藻類・プロティストフォーラム, Oct. 2013, Japanese, Domestic conference

  [Invited]

  Nominated symposium


• 新たな挑戦:イノベーティブ・バイオプロダクション

  蓮沼 誠久, 近藤 昭彦

  INCHEM TOKYO 2013 産学官マッチングフォーラム, Oct. 2013, Japanese, Domestic conference

  [Invited]

  Nominated symposium


• 緑藻Chlamydomonas orbicularisを用いた海水塩存在下での油脂高生産条件の開発

  中西 昭仁, 賀 詩欣, 藍川 晋, 張 嘉修, KONDO AKIHIKO, HASUNUMA TOMOHISA

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• 統合型リグノセルロース系エタノール生産プロセスに資する新規酵母細胞表層提示システムの開発

  猪熊 健太郎, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• 統合バイオプロセスによる環境調和型セルロース系エタノール生産に資する前処理技術の開発

  崎濱 由梨, 俣野 結城, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• 海洋性シアノバクテリアによるバイオリファイナリーのためのグリコーゲン生産

  西田 篤実, 藍川 晋平, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• 海水中のイオン濃度が糖質源Spirulinaの増殖;光合成に与える影響

  藍川 晋平, 秋元 誠志, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• 稲わら希硫酸処理液のナノフィルトレーションによる糖濃縮

  SASAKI KENGO, HASUNUMA TOMOHISA, OGINO CHIAKI, KONDO AKIHIKO

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• キシロース資化性Saccharomyces cerevisiaeにおける代謝プログラム制御によるギ酸;酢酸耐性関連遺伝子の同定

  阪本 貴俊, 堀 良美, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• キシロース資化性Saccharomyces cerevisiaeにおけるリン酸シグナル伝達系の制御によるキシロースからのエタノール生産の高効率化

  HASUNUMA TOMOHISA, KONDO AKIHIKO

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• Phototrophic cultivation of a marine microalga Chlamydomonas orbicularis CO2 fixation and biodiesel production:Effect of medium composition, nitrogen deplection, and sea salt concentration

  HO Shih-Hsin, NAKANISHI Akihito, AIKAWA Shimpei, CHANG Jo-Shu, KONDO Akihiko, HASUNUMA Tomohisa

  第65回日本生物工学会大会, Sep. 2013, Japanese, 日本生物工学会, 広島市, Domestic conference

  Poster presentation


• メタボローム解析による次世代バイオ燃料生産のための微細藻代謝改変戦略の導出

  蓮沼 誠久

  微細藻類研究会2013, Jun. 2013, Japanese, Domestic conference

  [Invited]

  Public discourse


• Direct conversion of cyanobacteria to ethanol without pretreatment or enzymatic hydrolysis process

  AIKAWA S, YAMADA R, YAMGISHI T, CHNG J.S, HASUNUMA Tomohisa, KONDO Akihiko

  The 3rd International Conference on Algal Biomass、Biofuels and Bioproducts, Jun. 2013, English, Elsevier, Toronto, Canada, International conference

  Poster presentation


• シアノバクテリアからの直接バイオエタノール変換技術の開発

  藍川 晋平, 山田 亮祐, 和泉 自泰, 山岸 隆博, 松田 史生, 川井 浩史, Jo-shu Chang, HASUNUMA TOMOHISA, KONDO AKIHIKO

  第４回日本光合成学会年会, May 2013, Japanese, 日本光合成学会, 名古屋市, Domestic conference

  Poster presentation


• Development of Microbial Cell Factories for Bio-refinery

  KONDO Akihiko, HASUNUMA Tomohisa

  Internatinal meeting of the Microbiorogical Society of Korea, May 2013, English, Microbiorogical Society of Korea, Jeonju, Korea, International conference

  Oral presentation


• 微細藻バイオリファイナリーに資する動的代謝プロファイリング解析

  HASUNUMA TOMOHISA

  日本農芸化学会2013年度大会, Mar. 2013, Japanese, 日本農芸化学会, 仙台市, Domestic conference

  Oral presentation


• 発酵阻害物存在下におけるキシロースからのエタノール発酵向上酵母の構築

  崎濱 由梨, HASUNUMA TOMOHISA, KONDO AKIHIKO

  日本農芸化学会2013年度大会, Mar. 2013, Japanese, 日本農芸化学会, 仙台市, Domestic conference

  Oral presentation


• 耐塩性シアノバクテリアArthrospira platensisからの効率の良いグリコーゲン回収法の構築

  中西 昭仁, 藍川 晋平, HASUNUMA TOMOHISA, KONDO AKIHIKO

  日本農芸化学会2013年度大会, Mar. 2013, Japanese, 日本農芸化学会, 仙台市, Domestic conference

  Oral presentation


• 神戸大学におけるバイオリファイナリーの進展

  HASUNUMA TOMOHISA, KONDO AKIHIKO

  化学工学会第78年会, Mar. 2013, Japanese, 化学工学会, 豊中市, Domestic conference

  Oral presentation


• リグノセルロース系バイオマスからの統合型バイオエタノール生産に資する新規酵母細胞表層提示システムの開発

  猪熊 健太郎, HASUNUMA TOMOHISA, KONDO AKIHIKO

  日本農芸化学会2013年度大会, Mar. 2013, Japanese, 日本農芸化学会, 仙台市, Domestic conference

  Oral presentation


• Biorefinery from microalgae through fermentation and metabolic engineering

  Tomohisa Hasunuma

  SBJ 64th Annual Meeting, Oct. 2012, English, International conference

  [Invited]

  Invited oral presentation


• リパーゼ分泌挙動の異なる糸状菌Rhizopus oryzae のメタボローム解析

  吉田 あゆみ, 濵 真司, HASUNUMA TOMOHISA, 山本 博之, KONDO AKIHIKO

  第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference

  Oral presentation


• イオン液体前処理とキシロース資化性酵母株を用いたバイオマス糖化発酵プロセスの効率化

  小倉 一真, 仲島 菜々実, YAMADA RYOSUKE, HASUNUMA TOMOHISA, 神谷 典穂, 石田 亘広, 齋藤 聡志, OGINO CHIAKI

  第64回日本生物工学会大会, Oct. 2012, Japanese, 日本生物工学会, 神戸市, Domestic conference

  Oral presentation


• Development of sustainable biorefinery based on microalgae

  HASUNUMA Tomohisa

  5th International Conference on Industrial Bioprocesses (IFIB-2012), Oct. 2012, English, National Taiwan University of Science and Technology, Taiwan, China, International conference

  Oral presentation


• Flux balance analysis of xylose-fermenting Saccharomyces cerevisiae strain

  KATO Hiroko, NAGATA Kento, HASUNUMA Tomohisa, MATSUDA Fumio, KONDO Akihiko

  The 15th International Biotechnology Symposium (IBS 2012), Sep. 2012, English, The Korean Society for Biotechnology and Bioengineering, Deague, Korea, International conference

  Oral presentation


• Cell recycle batch fermentation of high‐solid lignocellulose using a recombinant cellulase‐displaying yeast strain for high yield ethanol production

  HASUNUMA Tomohisa, KONDO Akihiko

  The 13th International Congress on Yeasts, Aug. 2012, English, ICY 2012 Organizing Committee, Madison, USA, International conference

  Oral presentation


• バイオリファイナリー構築に資する微生物細胞工場の創製

  HASUNUMA TOMOHISA

  アジレント・メタボロミクスセミナー2012, Jul. 2012, Japanese, アジレント・テクノロジー㈱, 豊中市, Domestic conference

  Oral presentation


• Control of phosphate metabolism in a xylose-fermenting yeast strain improves ethanol production from xylose

  HASUNUMA Tomohisa

  Metabolic EngineeringⅨ, Jun. 2012, English, Engineering Conferences International, Biarritz, France, International conference

  Oral presentation


• Microbial engineering for production of biofuels and biorefinery products

  Tomohisa Hasunuma

  4th A-IMBN Annual Conference, Mar. 2012, English, International conference

  [Invited]

  Invited oral presentation


• シアノバクテリアArthrospira platensisを炭素源としたアミラーゼ発現酵母による直接エタノール生産

  藍川 晋平, 和泉 自泰, 山田 亮祐, 松田 史生, 蓮沼 誠久, 近藤 昭彦

  日本農芸化学会2012年度大会, Mar. 2012, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• Metabolic pathway engineering in cyanobacteria Synechocystis sp.PCC 6803 for the production of lactate

  JOSEPH Ancy, AIKAWA Shimpei, HASUNUMA Tomohisa, MATSUDA Fumio, KONDO Akihiko

  日本農芸化学会2012年度大会, Mar. 2012, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• イオン液体共存下における酵母のキシロース発酵能評価

  小倉 一真, 仲島 菜々実, 山田 亮祐, 蓮沼 誠久, 荻野 千秋, 近藤 昭彦

  第2回イオン液体討論会, Dec. 2011, Japanese, イオン液体研究会, 京都市, Domestic conference

  Oral presentation


• Production of bio-ethanol from microalgae through cell surface engineering

  Tomohisa Hasunuma, Akihiko Kondo

  5th International Algae Congress 2011, Dec. 2011, English, DLG BENELUX, Berlin, Germany, International conference

  Oral presentation


• 補助食品・スピルリナのメタボローム解析

  蓮沼 誠久

  LECOジャパン・食品分析セミナー, Nov. 2011, Japanese, Domestic conference

  [Invited]

  Public discourse


• CE-TOFMSを用いたバイオエタノール生産酵母の代謝プロファイリング

  蓮沼 誠久

  第31回キャピラリー電気泳動シンポジウム, Nov. 2011, Japanese, 日本分析化学会電気泳動分析研究懇談会, 山形県鶴岡市, Domestic conference

  Oral presentation


• 発酵阻害物耐性遺伝子を導入したキシロース資化性二倍体酵母によるリグノセルロース水熱分解液の繰返し発酵

  蓮沼 誠久, 近藤 昭彦

  第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference

  Oral presentation


• 細胞内酸化還元バランス調整能を付与したキシロース資化性酵母の取得

  須賀 裕之, 蓮沼 誠久, 松田 史生, 近藤 昭彦

  第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference

  Oral presentation


• 回転式発酵槽を用いた稲わら水熱処理物からのエタノール生産の効率化

  俣野 結城, 蓮沼 誠久, 近藤 昭彦

  第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference

  Oral presentation


• ユーグレナによるバイオ燃料生産のための高密度CO2 固定サイクルの構築

  林 雅弘, 松田 史生, 蓮沼 誠久, 近藤 昭彦

  第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference

  Oral presentation


• メタボロミクスによるリン酸代謝関連遺伝子欠損酵母の発酵特性の解析

  藤冨 桂介, 蓮沼 誠久, 近藤 昭彦

  第3回 神戸大学バイオサイエンス研究会・若手研究者交流会, Sep. 2011, Japanese, 神戸大学バイオサイエンス研究会・若手研究者交流会, 神戸市, Domestic conference

  Poster presentation


• フラン化合物存在下でのXR-XDH 発現酵母を用いたキシロース発酵におけるNADH 依存性Adh1過剰発現のインパクト

  石井 純, 吉村 一也, 蓮沼 誠久, 近藤 昭彦

  第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference

  Oral presentation


• キシロース資化性酵母Saccharomyces cerevisiae におけるPHO13 遺伝子欠損がエタノール生産に与える影響及び発酵阻害物耐性の評価

  藤冨 桂介, 蓮沼 誠久, 近藤 昭彦

  第63回日本生物工学会大会, Sep. 2011, Japanese, 日本生物工学会, 小金井市, Domestic conference

  Oral presentation


• シンセティックバイオエンジニアリングによるバイオエタノール生産酵母の育種

  蓮沼 誠久

  第99回 醗酵学懇話会, Aug. 2011, Japanese, 日本生物工学会 関西支部, 神戸市, Domestic conference

  Invited oral presentation


• Repeated batch-fermentation of rice straw hydrolysate to ethanol using metabolically engineered Saccharomyces cerevisiae strains

  HASUNUMA Tomohisa, KONDO Akihiko

  SIM2011 Annual Meeting and Exhibition, Jul. 2011, English, Society for Industrial Microbiology, New Orleans, USA, International conference

  Oral presentation


• Development of microbial cell factories for biofuel production from biomass by conslidated bioprocessing

  KONDO Akihiko, HASUNUMA Tomohisa

  SIM2011 Annual Meeting and Exhibition, Jul. 2011, English, Society for Industrial Microbiology, New Orleans, USA, International conference

  Oral presentation


• 合成生物工学によるバイオ燃料生産のための微生物細胞工場の創製

  蓮沼 誠久

  バイオマスの今、そして未来, Jun. 2011, Japanese, Domestic conference

  [Invited]

  Public discourse


• Development of Microbial Cell Factories for Biofuel Production from Biomass

  Tomohisa Hasunuma, Chiaki Ogino, Akihiko Kondo

  2011 International Conference for Bioeconomy, Jun. 2011, English, Tianjin Institute of Scientific and Technical Information, Tianjin、China, International conference

  Oral presentation


• Cellulosic ethanol Production in Saccharomyces cerevisiae through Metabolic Engineering Based on Metabolomic Approach

  HASUNUMA Tomohisa

  Asian Congress on Biotechnology 2011, May 2011, English, ACB-2011Organizing Committee, Shanghai, China, International conference

  Oral presentation


• セルラーゼ表層提示株を用いた稲わら水熱処理物からの エタノール生産プロセスの効率化

  俣野 結城, HASUNUMA Tomohisa, KONDO Akihiko

  日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• セルラーゼを表層提示した高温耐性酵母Kluyveromyces marxianusを用いたセルロースからのエタノール生産

  HASUNUMA Tomohisa, 柳瀬 修平, YAMADA Ryosuke, KONDO Akihiko

  日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• キシロース発酵形質転換酵母における代謝コントロールの解析

  MATSUDA Fumio, 加藤 寛子, HASUNUMA Tomohisa, KONDO Akihiko

  日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• PHO13欠損株を用いたキシロースからのエタノール生産

  藤冨 桂介, 三田 智也, HASUNUMA Tomohisa, MATSUDA Fumio, KONDO Akihiko

  日本農芸化学会2011年度大会, Mar. 2011, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• PHO13欠損株を用いたキシロースからのエタノール生産

  藤冨 桂介, 三田 智也, HASUNUMA Tomohisa, KONDO Akihiko

  化学工学会第15回学生発表会, Mar. 2011, Japanese, (社)化学工学会, 神戸市, Domestic conference

  Oral presentation


• Production of bio-ethanol from lignocellulosic materials through the engineering of yeast cell surface and metabolic pathway

  Tomohisa Hasunuma

  4th Japan-Korea Biomass Symposium, Nov. 2010, English, International conference

  [Invited]

  Invited oral presentation


• 有用希少イノシトールのバイオアベイラビリティーとバイオコンバージョン生産

  YOSHIDA Ken-ichi, HASUNUMA Tomohisa

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Oral presentation


• 微細藻を利用したバイオ燃料生産システムの構築へ向けて

  KONDO Akihiko, HASUNUMA Tomohisa

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Oral presentation


• 発酵阻害物存在下におけるキシロースからの効率的なエタノール生産

  三田 智也, YAMADA Ryosuke, HASUNUMA Tomohisa, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• 海洋性微細藻のシステムバイオロジー解析に向けての網羅的代謝プロファイリング技術の開発

  和泉 自泰, 藍川 晋平, MATSUDA Fumio, HASUNUMA Tomohisa, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• ワイン酵母OC-2 トリプルマーカー株をプラットフォームとした高β – グルコシダーゼ活性と高キシロシダーゼ活性を持つキシロース発酵酵母の作製

  齋藤 聡志, TANAKA Tsutomu, HASUNUMA Tomohisa, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• ラン藻Spirulina (Arthrospira) platensis のグリコーゲン蓄積量と増殖速度に対する光強度の影響

  藍川 晋平, 和泉 自泰, MATSUDA Fumio, HASUNUMA Tomohisa, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• キシロース資化性形質転換酵母の代謝解析

  MATSUDA Fumio, 加藤 寛子, HASUNUMA Tomohisa, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• キシロース資化性Saccharomyces cerevisiae におけるアルコールデヒドロゲナーゼ過剰発現およびフラン化合物存在下でのキシロース発酵特性

  吉村 一也, ISHII Jun, HASUNUMA Tomohisa, KONDO Akihiko

  第62回日本生物工学会大会, Oct. 2010, Japanese, (社)日本生物工学会, 宮崎市, Domestic conference

  Poster presentation


• メタボロームを利用した新規バイオエタノール生産酵母の育種

  HASUNUMA Tomohisa

  第5回メタボロームシンポジウム, Sep. 2010, Japanese, メタボロームシンポジウム実行委員会, 山形県鶴岡市, Domestic conference

  Oral presentation


• Production of bio-ethanol from lignocellulosic materials through engineering yeast cell surface and metabolic pathway

  KONDO Akihiko, MATSUDA Fumio, HASUNUMA Tomohisa

  SIM 2010 Annual Meeting, Aug. 2010, English, Society for Industrial Microbiology, San Francisco, USA, International conference

  Oral presentation


• Ethanol production from cellulosic material using the thermotolerant yeast displaying cellulolytic enzymes on the cell surface

  HASUNUMA Tomohisa, YANASE, S, YAMADA Ryosuke, KONDO Akihiko

  SIM 2010 Annual Meeting, Aug. 2010, English, Society for Industrial Microbiology, San Francisco, USA, International conference

  Oral presentation


• Development of bioethanol production from marine microalgae

  HASUNUMA Tomohisa

  The 1st International Symposium on the Nitrogen Nutrition of Plants, Jul. 2010, English, Nitrogen 2010 Organizing Committee, 犬山市, International conference

  Poster presentation


• Development of bioethanol production from marine microalgae

  HASUNUMA Tomohisa, AIKAWA, S, IZUMI, Y, MATSUDA Fumio, KONDO Akihiko

  2010 International Symposium on Advanced Biological Engineering, Jul. 2010, English, Biotechnology for Sustainable Industry and Society, Beijing, China, International conference

  Oral presentation


• Ammonium assimilation in rice leaves: functions of the chloroplastic andcytosolic phosphoenolpyruvate carboxylases (PEPCs) in supplying carbonskeletons

  MIYAZAWA, S, MASUMOTO, C, HASUNUMA Tomohisa, MIYIAO, M

  The 1st International Symposium on the Nitrogen Nutrition of Plants, Jul. 2010, English, Nitrogen 2010 Organizing Committee, 犬山市, International conference

  Poster presentation


• Metabolomic approach to identify molecules that are important for acetic acid tolerance in a recombinant xylose-fermenting yeast strain

  HASUNUMA Tomohisa

  Metabolic Engineering VIII: Metabolic Engineering for Green Growth, Jun. 2010, English, Engineering Conferences International, Jeju、Korea, International conference

  Oral presentation


• Efficient Production of Cellulosic Ethanol in Saccharomyces cerevisiae through Metabolic Engineering Based on a Metabolomic Approach

  HASUNUMA Tomohisa

  Microbes at Work JSPS-SLU colloquium, Jun. 2010, English, JSPS-SLU, Uppsala, Sweden, International conference

  Oral presentation


• Construction of consolidated bioprocesses for production of bio-fuels and chemicals by using cell surface engineered microbial cells

  KONDO Akihiko, HASUNUMA Tomohisa, OGINO Chiaki, TANAKA Tsutomu

  RENEWABLE ENERGY 2010, Jun. 2010, English, International Solar Energy Society, 横浜市, International conference

  Oral presentation


• 酢酸耐性酵母によるキシロースからの効率的バイオエタノール生産

  三田 智也, 山田 亮祐, 蓮沼 誠久, 近藤 昭彦

  日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference

  Oral presentation


• 細胞表層工学技術を用いたヘミセルロース系バイオマスからのエタノール生産

  阪本 貴俊, 蓮沼 誠久, 堀 良美, 山田 亮祐, 荻野 千秋, 近藤 昭彦

  日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference

  Oral presentation


• バイオマスからの燃料・化学品生産を目指した細胞工場の創製

  近藤 昭彦, 荻野 千秋, 蓮沼 誠久, 田中 勉

  日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference

  Oral presentation


• キシロース資化性遺伝子組換え酵母の代謝フラックス解析

  松田 史生, 三田 智也, 蓮沼 誠久, 近藤 昭彦

  日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference

  Oral presentation


• D-アラビノースは酵母のエリスロアスコルビン酸の内在前駆体か

  尼子 克己, 森村 健一, 蓮沼 誠久, 近藤 昭彦, 合田 清

  日本農芸化学会2010年度大会, Mar. 2010, Japanese, (社)日本農芸化学会, 東京都目黒区, Domestic conference

  Oral presentation


• A synthetic biology approach for efficient production of cellulosic ethanol in Saccharomyces cerevisiae

  Tomohisa Hasunuma

  The 2nd International Symposium of Innovative BioProduction Kobe, Jan. 2010, English, International conference

  Invited oral presentation


• Effective biofuel production process from biomass reseources by whole-cell biocatalyst

  Tomohisa Hasunuma

  Airbus meeting, Nov. 2009, English, International conference

  [Invited]

  Invited oral presentation


• アーミング酵母を用いた統合プロセスによるバイオエタノール生産

  蓮沼 誠久

  日本化学会関東支部講演会 「バイオマス変換最前線」, Nov. 2009, Japanese, Domestic conference

  [Invited]

  Invited oral presentation


• Dynamic metabolic profiling in plant leaves using in vivo stable isotope labeling

  蓮沼 誠久, 近藤 昭彦, 福崎 英一郎

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Oral presentation


• Bioethaol fermentation from mixed sugar by the recombinant yeast with xyloseisomerase pathway

  TANINO Takanori, HOTTA Atsushi, ITO Tomonori, ISHII Jun, YAMADA Ryousuke, HASUNUMA Tomohisa, OGINO Chiaki, OHMURA Naoto

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• Bioethanol production from mixed sugars using sugar uptake ability enhanced yeast strain by overexpression of transporters

  堀田 淳史, 谷野 孝徳, 伊藤 友数, 蓮沼 誠久, 荻野 千秋, 近藤 昭彦, 大村 直人

  APBioChEC'09, Nov. 2009, English, APBioChEC'09 Committee, 神戸市, International conference

  Poster presentation


• バイオエタノール生産を目指したスーパー酵母の創製

  近藤 昭彦, 蓮沼 誠久, 田中 勉, 荻野 千秋

  農芸化学会2009年度大会, Mar. 2009, Japanese, 日本農芸化学会, 福岡県福岡市, Domestic conference

  Oral presentation


• キシロースからの効率的なエタノール生産を行う酵母の創製

  KATO Hiroko, YAMADA Ryosuke, HASUNMA Tomohisa, FUKUDA Hideki, KONDO Akihiko

  化学工学会第74年会, Mar. 2009, Japanese, 化学工学会, 横浜市, Domestic conference

  Oral presentation


• C3 photosynthetic pathway analysis by combination of in vivo 13C-labeling and metabolic profiling

  HASUNMA Tomohisa

  NAISTグローバルCOE公開ワークショップ「光合成の環境応答」, Mar. 2009, Japanese, 日本分子生物学会・日本生化学会, 奈良県生駒市, Domestic conference

  [Invited]

  Invited oral presentation


• 13CO2を用いた同位体標識による葉内代謝物質のターンオーバー解析

  HASUNMA Tomohisa, HARADA Kazuo, MIYAKE Chikahiro, FUKUSAKI Ei-ichiro

  日本分子生物学会年会・日本生化学会大会 合同大会, Dec. 2008, Japanese, 日本分子生物学会・日本生化学会, 神戸市, Domestic conference

  [Invited]

  Invited oral presentation


• in vivo安定同位体濃縮による葉内炭素代謝ターンオーバー解析

  HASUNMA Tomohisa

  メタボロームシンポジウム, Oct. 2008, Japanese, 慶應義塾大学先端生命科学研究所, 山形県鶴岡市, Domestic conference

  Poster presentation


• in vivo 13Cエンリッチメントによる葉内炭素代謝解析システムの構築

  HASUNMA Tomohisa

  関西光合成研究会シンポジウム, Sep. 2008, Japanese, 関西光合成研究会, 神戸市, Domestic conference

  [Invited]

  Invited oral presentation


• Development of analytical system to monitor metabolic turnoverin plant leaves using in vivo isotope dilution from 13CO2

  OHYAMA Yohko, HASUNMA Tomohisa, HARADA Kazuo, MIYAKE Chikahiro, KOBAYASHI Akio, FUKUSAKI Ei-ichiro

  5th International conference on plant metabolomics, Jul. 2008, English, RIKEN Plant Science Center, 横浜市, Domestic conference

  Poster presentation


• 葉緑体形質転換技術を用いたアスタキサンチン生産

  HASUNMA Tomohisa, MIYAZAWA Shin-ichi, YOSHIMURA Satomi, SHINZAKI Yuki, TOMIZAWA Ken-ichi, MISAWA Norihiko, MIYAKE Chikahiro

  日本農芸化学会大会, Mar. 2008, Japanese, 日本農芸化学会, 名古屋市, Domestic conference

  Oral presentation


• CE/MSを用いた一次代謝物の安定同位体標識率モニタリング法の開発

  HARADA Kazuo, OHYAMA Yohko, HASUNMA Tomohisa, MIYAZAWA Shin-ichi, MIYAKE Chikahiro, KOBAYASHI Akio, FUKUSAKI Ei-ichiro

  日本農芸化学会大会, Mar. 2008, Japanese, 日本生物工学会, 名古屋市, Domestic conference

  Oral presentation


• 13CO2エンリッチメントによる代謝ターンオーバー解析

  HASUNMA Tomohisa, FUKUSAKI Ei-ichiro, OKAZAWA Atsushi, KAJIYAMA Shin-ichiro, KOBAYASHI Akio

  日本植物生理学会年会, Mar. 2008, Japanese, 日本植物生理学会, 札幌市, Domestic conference

  Oral presentation


• 主要カロテノイドとしてアスタキサンチンを生産する葉緑体形質転換植物の作出

  HASUNMA Tomohisa, YOSHIMURA Satomi, MIYAZAWA Shin-ichi, MISAWA Norihiko, MIYAKE Chikahiro

  カロテノイド研究談話会, Sep. 2007, Japanese, 日本カロテノイド研究会, 大阪市, Domestic conference

  Oral presentation


• 葉緑体形質転換技術を用いたカロテノイド代謝改変植物の作出

  HASUNMA Tomohisa, KAWASAKI Satomi, YAMAMOTO Hiroshi, MIYAKE Chikahiro, FUKUSAKI Ei-ichiro, TOMIZAWA Ken-ichi

  カロテノイド研究談話会, Sep. 2006, Japanese, 日本カロテノイド研究会, 沖縄県, Domestic conference

  Oral presentation


• ラン藻Synechosystis sp.由来デオキシキシルロースリン酸レダクトイソメラーゼを導入した葉緑体形質転換タバコの作出

  HASUNMA Tomohisa, KAWASAKI Satomi, YAMAMOTO Hiroshi, TAKASE Hisabumi, TAKENO Shinya, BAMBA Takeshi, FUKUSAKI Ei-ichiro, KOBAYASHI Akio, TOMIZAWA Ken-ichi

  日本農芸化学会大会, Mar. 2005, Japanese, 日本農芸化学会, 京都市, Domestic conference

  Oral presentation


• 安定同位体希釈法による定量メタボローム解析法の開発(2)-15N標識による含窒素化合物群の相対定量法-

  FUKUSAKI Ei-ichiro, KIM Jae Kwang, HASUNMA Tomohisa, HARADA Kazuo, KOBAYASHI Akio

  日本農芸化学会大会, Mar. 2004, Japanese, 日本農芸化学会, 広島市, Domestic conference

  Oral presentation


• Aspergillus niger由来ペクチンメチルエステラーゼの植物内過剰発現によるメタノール増産と植物矮化

  HASUNMA Tomohisa, FUKUSAKI Ei-ichiro, OKAZAWA Atsushi, KAJIYAMA Shin-ichiro, KOBAYASHI Akio

  日本生物工学会大会, Sep. 2003, Japanese, 日本生物工学会, 熊本市, Domestic conference

  Oral presentation


• アルコールオキシダーゼ・ペルオキシダーゼ二酵素型バイオセンサーを用いたメタノール定量測定

  HASUNMA Tomohisa, FUKUSAKI Ei-ichiro, OKAZAWA Atsushi, KAJIYAMA Shin-ichiro, KUWABATA Susumu, KOBAYASHI Akio

  日本農芸化学会大会, Mar. 2003, Japanese, 日本農芸化学会, 神奈川県藤沢市, Domestic conference

  Oral presentation


• Aspergillus niger由来ペクチンメチルエステラーゼの植物内過剰発現によるメタノール産生

  HASUNMA Tomohisa, FUKUSAKI Ei-ichiro, OKAZAWA Atsushi, KAJIYAMA Shin-ichiro, KOBAYASHI Akio

  日本農芸化学会大会, Mar. 2002, Japanese, 日本農芸化学会, 仙台市, Domestic conference

  Oral presentation


• チューブリン分子に特異的に結合するDNAアプタマー

  HASUNMA Tomohisa, FUKUSAKI Ei-ichiro, OKAZAWA Atsushi, KAJIYAMA Shin-ichiro, KOBAYASHI Akio

  日本生物工学会大会, Aug. 2000, Japanese, 日本生物工学会, 札幌市, Domestic conference

  Oral presentation

• 大腸菌による芳香族化合物発酵の飛躍的効率化につながるバイオマス由来発酵阻害の克服

  川口 秀夫

  学術研究助成基金助成金／基盤研究(C), Apr. 2016 - Mar. 2021

  Competitive research funding


• 海洋性ラン藻における人工転写調節システムの開発とイソプレノイド生産への応用

  蓮沼 誠久

  科学研究費補助金／若手研究(A), Apr. 2015 - Mar. 2018, Principal investigator

  Competitive research funding


• 微細藻類からのカロテノイド色素の生産技術開発

  蓮沼 誠久

  国立研究開発法人科学技術振興機構, 研究成果展開事業 研究成果最適展開支援プログラム(A-STEP）シーズ育成タイプ, 2017, Principal investigator

  Competitive research funding


• 【NEDO：三菱ケミカル】共同研究員受入

  近藤 昭彦

  三菱ケミカル株式会社, NEDO委託プロジェクト「植物等の生物を用いた高機能品生産技術の開発」, 2017

  Competitive research funding


• 【JAXA地上】微小重力環境における藻類による物質循環サイクルの実現可能性検証

  蓮沼 誠久

  一般財団法人日本宇宙フォーラム, 「きぼう」利用フィジビリティスタディテーマ募集, 2017, Principal investigator

  Competitive research funding


• 【JAXA装置】微小重力環境における藻類による物質循環サイクルの実現可能性検証

  蓮沼 誠久

  一般財団法人日本宇宙フォーラム, 「きぼう」利用フィジビリティスタディテーマ募集, 2017, Principal investigator

  Competitive research funding


• 【ImPACT】動的代謝解析による海洋性緑藻の油脂生合成発動メカニズムの解明と油脂高生産技術開発への応用

  蓮沼 誠久

  国立研究開発法人科学技術振興機構, 革新的研究開発推進プログラム（ImPACT）, 2017, Principal investigator

  Competitive research funding


• 【AMED】アセンブラーとしての癌/非癌幹細胞の機能解明とその制御技術の開発

  青井 貴之

  国立研究開発法人日本医療研究開発機構, 再生医療実現拠点ネットワークプログラム幹細胞・再生医学イノベーション創出プログラム, 2017

  Competitive research funding


• 【ALCA】ラン藻代謝改変株の代謝解析とコハク酸生産プロセスの検討

  蓮沼 誠久

  国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業 先端的低炭素化技術開発（ALCA）, 2017, Principal investigator

  Competitive research funding


• 微細藻類からのカロテノイド色素の生産技術開発

  蓮沼 誠久

  国立研究開発法人科学技術振興機構, 研究成果最適展開支援プログラム（A-STEP）, 2016, Principal investigator

  Competitive research funding


• 宇宙空間におけるユーグレナ等の藻類による物質循環サイクルの実現可能性検証

  蓮沼 誠久

  「きぼう」利用フィジビリティスタディー, 2016, Principal investigator

  Competitive research funding


• 【ImPACT】動的代謝解析による海洋性緑藻の油脂生合成発動メカニズムの解明と油脂高生産技術開発への応用

  蓮沼 誠久

  革新的研究開発推進プログラム（ImPACT）, 2016, Principal investigator

  Competitive research funding


• 【ALCA】ラン藻代謝改変株の代謝解析とコハク酸生産プロセスの検討

  蓮沼 誠久

  国立研究開発法人科学技術振興機構, 戦略的創造研究推進事業（ALCA）, 2016, Principal investigator

  Competitive research funding


• 海洋性緑藻による油脂生産技術の研究開発

  蓮沼 誠久

  新エネルギー・産業技術総合開発機構, 戦略的次世代バイオマスエネルギー利用技術開発事業（次世代技術開発）, 2015, Principal investigator

  Competitive research funding


• 海洋性緑藻による油脂生産技術の研究開発

  蓮沼 誠久

  独立行政法人新エネルギー・産業技術総合開発機構, 戦略的次世代バイオマスエネルギー利用技術開発事業（次世代技術開発）, 2014, Principal investigator

  Competitive research funding


• 海洋性緑藻による油脂生産技術の研究開発

  蓮沼 誠久

  独立行政法人新エネルギー・産業技術総合開発機構, 戦略的次世代バイオマスエネルギー利用技術開発事業（次世代技術開発）, 2013, Principal investigator

  Competitive research funding


• さきがけ「高増殖性微細藻の合成を目指した微細藻代謝フラックス制御機構の解明」

  蓮沼 誠久

  戦略的創造研究推進事業 個人型研究さきがけ, 2013, Principal investigator

  Competitive research funding


• 海洋性緑藻による油脂生産技術の研究開発

  蓮沼 誠久

  独立行政法人新エネルギー・産業技術総合開発機構, 戦略的次世代バイオマスエネルギー利用技術開発事業（次世代技術開発）, 2012, Principal investigator

  Competitive research funding


• さきがけ「高増殖性微細藻の合成を目指した微細藻代謝フラックス制御機構の解明」

  蓮沼 誠久

  戦略的創造研究推進事業 個人型研究さきがけ, 2012, Principal investigator

  Competitive research funding


• さきがけ「高増殖性微細藻の合成を目指した微細藻代謝フラックス制御機構の解明」

  蓮沼 誠久

  戦略的創造研究推進事業 個人型研究さきがけ, 2011, Principal investigator

  Competitive research funding


• 動的代謝プロファイリング解析による発酵阻害機構の解明と高ストレス耐性酵母の創製

  蓮沼 誠久

  科学研究費補助金／若手研究(B), 2010, Principal investigator

  Competitive research funding

• 油脂成分を製造する方法、及び藻類を使用して高級不飽和脂肪酸を製造する方法

  近藤 昭彦, 蓮沼 誠久, 賀 詩欣, 皆川 純, 西江 晴男, 太郎田 博之, 張 嘉修

  特願14/913618, 17 Mar. 2014, 特許10351882, 16 Jul. 2019

  Patent right


• サンプリング装置

  蓮沼 誠久, 長堀(虎井) 彩, 松本 好弘

  PCT/JP2019/027323, 10 Jul. 2019

  Patent right


• キャップ着脱装置、並びに、これを備えたサンプリング装置及び前処理装置

  PCT/JP2019/027324, 10 Jul. 2019

  Patent right


• 攪拌装置及び前処理装置

  蓮沼 誠久, 長堀(虎井) 彩, 松本 好弘

  PCT/JP2019/027326, 10 Jul. 2019

  Patent right


• 攪拌装置及び前処理装置

  蓮沼 誠久, 長堀(虎井) 彩, 松本 好弘

  PCT/JP2019/027325, 10 Jul. 2019

  Patent right


• 固液界面検出装置及びこれを備えた前処理装置

  蓮沼 誠久, 渡邊 勉, 篁 直樹

  PCT/JP2019/027327, 10 Jul. 2019

  Patent right


• 分泌シグナルペプチドならびにそれを利用したタンパク質の分泌および細胞表層提示

  近藤 昭彦, 蓮沼 誠久, 猪熊 健太郎

  特願2016-538422, 30 Jul. 2015, 特許6537076, 14 Jun. 2019

  Patent right


• 油脂成分を産生する方法、高級不飽和脂肪酸の製造方法、及びクラミドモナス・スピーシーズＪＳＣ４株

  近藤 昭彦, 蓮沼 誠久, 賀 詩欣, 皆川 純, 西江 晴男, 太郎田 博之, 張 嘉修

  特願14838058.7, 17 Mar. 2014, 特許3037543, 29 May 2019

  Patent right


• クラミドモナス藻類から油脂成分を産生する方法

  近藤 昭彦, 蓮沼 誠久, 賀 詩欣, 皆川 純, 西江 晴男, 太郎田 博之, 張 嘉修

  特願14/913594, 17 Mar. 2014, 特許10214756, 26 Feb. 2019

  Patent right


• 組換え宿主細胞及びＤ－ブタントリオールの新規製造方法

  蓮沼 誠久, 近藤 昭彦, 番場 崇弘

  PCT/JP2019/004582, 08 Feb. 2019

  Patent right


• 油脂成分を産生する方法、高級不飽和脂肪酸の製造方法

  近藤 昭彦, 蓮沼 誠久, 賀 詩欣, 皆川 純, 西江 晴男, 太郎田 博之, 張 嘉修

  特願14837252.7, 17 Mar. 2014, 特許3037542, 13 Jun. 2018

  Patent right


• 細胞表層発現用ポリヌクレオチド

  KONDO AKIHIKO, HASUNUMA TOMOHISA, INOKUMA KENTARO

  特願2015-508514, 25 Mar. 2014, 大学長, 特許6335161, 11 May 2018

  Patent right


• バイオマスからのエタノールの生産方法

  KONDO AKIHIKO, HASUNUMA TOMOHISA, SAKIHAMA YURI

  特願2014-531686, 23 Aug. 2013, 大学長, 特許6236634, 10 Nov. 2017

  Patent right


• 有機酸の製造方法

  蓮沼 誠久, 松田 真実

  PCT/JP2017/032469, 08 Sep. 2017

  Patent right


• 有機酸の製造方法

  蓮沼 誠久, 松田 真実

  特願2018-539683, 08 Sep. 2017

  Patent right


• 細胞表層発現用ポリヌクレオチド（米国）

  KONDO AKIHIKO, HASUNUMA TOMOHISA, INOKUMA KENTARO

  14/778250, 25 Mar. 2014, 大学長, 9751917, 05 Sep. 2017

  Patent right


• 前処理済みの植物性バイオマスから発酵処理物を製造するための方法

  近藤 昭彦, 蓮沼 誠久, 荻野 千秋, 猪熊 健太郎, ギリモ グレゴリー, 渡部 啓吾, 安川 雄介, 金子 令治, 飯森 武志

  特願2017-77676, 10 Apr. 2017

  Patent right


• バイオマスからのエタノールの生産方法

  KONDO AKIHIKO, HASUNUMA TOMOHISA, 崎濱 由梨

  特願14/422728, 23 Aug. 2013, 大学長, 特許9441249, 13 Sep. 2016

  Patent right


• エタノールの新規生産方法

  KONDO AKIHIKO, HASUNUMA TOMOHISA

  特願2013-522836, 25 Jun. 2012, 大学長, 特許5935020, 20 May 2016

  Patent right


• 有機酸の製造方法

  蓮沼 誠久

  特願2017-511006, 06 Apr. 2016

  Patent right


• バイオマスからのエタノールの生産方法

  KONDO AKIHIKO, HASUNUMA TOMOHISA, 三田 智也

  特願2011-543349, 29 Nov. 2010, 大学長, 特許5804327, 11 Sep. 2015

  Patent right


• 油脂成分を産生する方法、及び高級不飽和脂肪酸の製造方法

  HASUNUMA TOMOHISA, KONDO AKIHIKO, HO SHIH-HSIN

  特願2014-541229, 17 Mar. 2014, 大学長, 特許5746796, 15 May 2015

  Patent right


• 油脂成分を産生する方法、高級不飽和脂肪酸の製造方法、及びクラミドモナス・スピーシーズＪＳＣ４株

  KONDO AKIHIKO, HASUNUMA TOMOHISA, HO SHIH-HSIN

  特願2014-550560, 17 Mar. 2014, 大学長, 特許5719977, 27 Mar. 2015

  Patent right


• エタノールの生産方法

  KONDO AKIHIKO, HASUNUMA TOMOHISA, AIKAWA SHINPEI

  特願2010-131364, 08 Jun. 2010, 大学長, 特許5626854, 10 Oct. 2014

  Patent right