Research activity information

• Sep. 2025 第133回日本畜産学会若手優秀発表賞, 子豚の腸管免疫機能を強化する枯草菌の役割とその分子メカニズム解明

  犬童優樹, 須田遊人, 生井 楓, 宗田吉広, 西川明芳, 宮崎綾子, 西山啓太, 北澤春樹


• Sep. 2010 NARO Research Prize 2010, 豚丹毒および豚マイコプラズマ肺炎を一度に予防できる経口ワクチン技術の開発

  小川洋介, 下地善弘, 宗田吉広


• Nov. 2009 日本農学進歩賞, 動物サイトカインの利活用による家畜疾病制御技術に関する研究

  宗田 吉広


• 2002 日本獣医学会獣医学奨励賞および大会長賞

  宗田 吉広

• 無菌豚、HPCD豚及びノトバイオート豚とそれらを活用した豚の糞便細菌叢移植及び異種移植に関する最近の話題と研究

  宗田吉広, 青木玲二, 西川明芳, 萩 達朗, 須田遊人, 宮崎綾子

  Lead, Aug. 2026, 日本豚病研究会報, (88) (88), Japanese

  [Refereed]


• Dynamics of tryptophan metabolites and microbial adaptations during corn by-product fermentation in the pig gut microbiome

  Eita Toyoshi, Masahiro Watanabe, Fu Namai, Kenji Yamane, Toma Kashima, Wakako Ikeda-Ohtsubo, Afifah Zahra Agista, Ayu Yoshida, Taiga Sakuma, Itsuko Fukuda, Kasumi Suzuki, Gou Yoshioka, Yuji Imai, Sae Tsuchida, Eri Nishiyama, Hiroki Shinkai, Yoshihiro Muneta, Hirohide Uenishi, Ryuta Tobe, Hitoshi Shirakawa, Masamitsu Maekawa, Nariyasu Mano, Haruki Kitazawa, Keita Nishiyama

  Abstract Background Food by-products, such as corn germ meal from starch processing, are increasingly used as sustainable feed supplements, reducing competition between food and feed and supporting the valorisation of food waste. However, their effects on gut microbial metabolism and host health remain unclear. This study aimed to determine how corn germ meal fermentation influences microbial community structure and metabolite production using an ex vivo pig faecal culture system. Results Corn germ meal supplementation significantly altered the microbial composition, increasing diversity and enriching fibre-degrading Prevotellaceae, a key bacterial family involved in complex carbohydrate metabolism. Metabolomic analysis revealed marked increases in tryptophan-derived metabolites, including indoleacrylic acid, indolepropionic acid, and indolelactic acid, which act as ligands for the aryl hydrocarbon receptor and have anti-inflammatory properties. Prevotella -mediated catabolite repression reduced Escherichia coli –derived indole formation, redirecting microbial tryptophan metabolism toward the production of these bioactive compounds. Microbial and metabolic responses differed among farms, reflecting farm-specific microbiome structures. Conclusions Corn germ meal supplementation reshapes gut microbial communities, enhances metabolic activity, and promotes the generation of bioactive tryptophan metabolites with potential immunomodulatory effects. These findings highlight the value of corn by-products as dietary fibres that can drive beneficial microbial cross-feeding and influence host intestinal homeostasis. Although demonstrated in an ex vivo setting, this study provides a mechanistic basis and preclinical evidence for future in vivo studies, supporting the sustainable utilisation of food industry by-products to improve gut health and resource efficiency in livestock production.

  Springer Science and Business Media LLC, Feb. 2026, Journal of Animal Science and Biotechnology, 17(1) (1)

  [Refereed]

  Scientific journal


• Polymer-Coated Resonant Sensors for Monitoring Volatile Fatty Acids in the Cow Rumen

  Fatin Bazilah Fauzi, Shintaro Noda, Michitaka Yamamoto, Jarred Fastier-Wooller, Yoshihiro Muneta, Shozo Arai, Toshihiro Itoh, Naoki Shiraishi

  Institute of Electrical and Electronics Engineers (IEEE), Feb. 2026, IEEE Sensors Letters, 10(2) (2), 1 - 4, English

  [Refereed]

  Scientific journal


• ICEmST contributes to colonization of Salmonella in the intestine of piglets

  Nobuo Arai, Tomoyuki Shibahara, Reina Nishiura, Yukino Tamamura-Andoh, Hayate Nishiura, Yoshihiro Muneta, Hiroshi Sawada, Ayako Watanabe-Yanai, Taketoshi Iwata, Masato Akiba, Masahiro Kusumoto

  Springer Science and Business Media LLC, Dec. 2024, Scientific Reports, 14(1) (1)

  [Refereed]

  Scientific journal


• Monitoring ventral tail base surface temperature for fever detection in calves.

  Tomomi Ozawa, Yuji Takahashi, Yoshihiro Muneta, Kaori Hoshinoo, Kumiko Kimura, Seijiro Tou, Shin Kakihara, Noriko Yamanaka, Toru Miyamoto, Shogo Higaki, Koji Yoshioka

  Jan. 2024, Anim Sci J, 95(1) (1), e13921

  [Refereed]


• Comparative studies on electrodes for rumen bacteria microbial fuel cells.

  Yusuke Yashiro, Michitaka Yamamoto, Yoshihiro Muneta, Hiroshi Sawada, Reina Nishiura, Shozo Arai, Seiichi Takamatsu, Toshihiro Itoh

  Apr. 2023, Sensors (Basel), 23(8) (8), 4162

  [Refereed]


• 豚のオミクス解析と疾病に関する最近の話題と研究

  宗田 吉広, 関山 恭代, 須田 智子, 宮崎 綾子, 新開 浩樹, 廣瀬 健右, 上西 博英, 北澤 春樹

  Lead, 日本豚病研究会, Feb. 2023, 日本豚病研究会報, (81) (81), 30 - 38, Japanese

  [Refereed]


• 牛ルーメン内容物発電槽由来の電力で作製した微酸性次亜塩素酸水の消毒効果

  松澤直樹, 塩原綾早, 中村南斗, 西森朝美, 澤田 浩, 岡本真理子, 高松大輔, 宗田吉広

  Corresponding, 2023, 日本家畜衛生学雑誌, 49, 71 - 77

  [Refereed]


• Stability evaluation of reference electrodes and indicating electrodes of pH sensor during cow's rumen condition monitoring.

  Chihiro Shimodan, Michitaka Yamamoto, Hiroshi Sawada, Yoshihiro Muneta, Shozo Arai, Hironao Okada, Seiichi Takamatsu, Toshihiro Itoh

  Jan. 2023, Sensors and Materials, 35(1) (1), 15 - 24

  [Refereed]


• Resting behaviour of steers after a low dose of lipopolysaccharide

  籾田康介, 吉田茉純, 宗田吉広, 石崎 宏, 桑原正貴, 小針大助, 矢用健一

  Jan. 2022, Animal Behavior and Management, 58(1) (1), 1 - 9

  [Refereed]


• 預託牧場での子牛の群導入時における体表温センサを用いた発熱検知

  安西真奈美, 大竹祥紘, 赤間俊輔, 米山洲二, 尾澤知美, 澤田 浩, 高橋雄治, 宮本 亨, 宗田吉広

  Corresponding, 2022, 日本獣医師会雑誌, 75, E1 - E8

  [Refereed]


• In vivo effect of a TLR5 SNP (C1205T) on Salmonella enterica serovar Typhimurium infection in weaned, specific pathogen-free Landrace piglets

  Yoshihiro Muneta, Nobuo Arai, Yoko Yakabe, Masahiro Eguchi, Tomoyuki Shibahara, Akiko Sakuma, Hiroki Shinkai, Hirohide Uenishi, Kensuke Hirose, Masato Akiba

  Lead, Wiley, Jun. 2018, Microbiology and Immunology, 62(6) (6), 380 - 387, English

  [Refereed]

  Scientific journal


• 血清中Interleukin-18（IL--18）の定期的なモニタリングは養豚農場の衛生状態を評価するための有用な1指標となりうるー2養豚農場の比較による実証試験の一例ー

  矢ヶ部陽子, 高萩陽一, 宗田吉広

  Corresponding, 2018, 農研機構動物衛生研究部門研究報告, 124, 1 - 8

  [Refereed]


• 放牧が黒毛和種牛の酸化ストレス、免疫及び栄養指標に及ぼす影響

  原恵, 山本伸治, 壁谷昌彦, 長谷川裕貴, 矢ヶ部陽子, 宗田吉広

  2018, 日本獣医師会雑誌, 71(8) (8), 437 - 442

  [Refereed]


• Immunostimulatory effects of recombinant Erysipelothrix rhusiopathiae expressing porcine interleukin-18 in mice and pigs.

  Ogawa Y, Minagawa Y, Shi F, Eguchi M, Muneta Y, Shimoji Y

  Interleukin-18 (IL-18), which was originally called gamma interferon (IFN-γ)-inducing factor, has been shown to play an important role in innate and acquired immune responses. In this study, attenuatedErysipelothrix rhusiopathiaestrains were engineered to produce porcine IL-18 (poIL-18) and evaluated for their potential immunostimulatory effect in animals. Recombinant poIL-18 was successfully expressed in the recombinantE. rhusiopathiaestrains YS-1/IL-18 and KO/IL-18. The culture supernatant of YS-1/IL-18 was confirmed to induce IFN-γ production in murine splenocytesin vitro, and this production was inhibited by incubation with anti-poIL-18 monoclonal antibodies. Furthermore, more IFN-γ production was induced upon stimulation of splenocytes with concanavalin A for splenocytes from mice that were intraperitoneally inoculated with YS-1/IL-18 than for splenocytes from control mice inoculated with the parent strain YS-1. Peritoneal macrophages from mice preinoculated with YS-1/IL-18 exhibited enhanced phagocytosis ofSalmonella entericasubsp.entericaserovar Typhimurium compared with peritoneal macrophages from control mice preinoculated with YS-1. We also confirmed the immunostimulatory effect on humoral immune responses against antigens ofE. rhusiopathiaeandMycoplasma hyopneumoniaein gnotobiotic pigs that were orally preinoculated with KO/IL-18. Thus, these results provide evidence thatE. rhusiopathiaeis a promising vector for the expression of host cytokines and suggest the potential utility ofE. rhusiopathiaevector-encoded cytokines in the activation of host innate and acquired immune responses.

  American Society for Microbiology, Sep. 2012, Clinical and vaccine immunology : CVI, 19(9) (9), 1393 - 1398

  [Refereed]

  Scientific journal


• Allele-specific primer polymerase chain reaction for a single nucleotide polymorphism (C1205T) of swine Toll-like receptor 5 and comparison of the allelic frequency among several pig breeds in Japan and the Czech Republic

  Yoshihiro Muneta, Yu Minagawa, Masahiro Kusumoto, Hiroki Shinkai, Hirohide Uenishi, Igor Splichal

  Lead, Jun. 2012, MICROBIOLOGY AND IMMUNOLOGY, 56(6) (6), 385 - 391, English

  [Refereed]

  Scientific journal


• Development of Allele-Specific Primer PCR for a Swine TLR2 SNP and Comparison of the Frequency among Several Pig Breeds of Japan and the Czech Republic

  Yoshihiro Muneta, Yu Minagawa, Masahiro Kusumoto, Hiroki Shinkai, Hirohide Uenishi, Igor Splichal

  Lead, May 2012, JOURNAL OF VETERINARY MEDICAL SCIENCE, 74(5) (5), 553 - 559, English

  [Refereed]

  Scientific journal


• Toll-like receptor 4 polymorphism impairing lipopolysaccharide signaling in Sus scrofa, and its restricted distribution among Japanese wild boar populations.

  Shinkai H, Okumura N, Suzuki R, Muneta Y, Uenishi H

  4, Apr. 2012, DNA and cell biology, 31(4) (4), 575 - 581

  [Refereed]


• Interleukin-18 expression in pig salivary glands and salivary content changes during acute immobilization stress

  Y. Muneta, Y. Minagawa, T. Nakane, T. Shibahara, T. Yoshikawa, Y. Omata

  Lead, Sep. 2011, STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS, 14(5) (5), 549 - 556, English

  [Refereed]

  Scientific journal


• Polymorphisms in pattern recognition receptors and their relationship to infectious disease susceptibility in pigs.

  Uenishi H, Shinkai H, Morozumi T, Muneta Y, Jozaki K, Kojima-Shibata C, Suzuki E

  Jun. 2011, BMC proceedings, 5 Suppl 4, S27

  [Refereed]


• Expression and Characterization of Bluetongue Virus Serotype 21 VP7 Antigen: C-Terminal Truncated Protein has Significantly Reduced Antigenicity

  Madhusudan Hosamani, Shinya Shimizu, Jiro Hirota, Takehiro Kokuho, Takayuki Kubota, Satoko Watanabe, Masato Ohta, Yoshihiro Muneta, Shigeki Inumaru

  May 2011, JOURNAL OF VETERINARY MEDICAL SCIENCE, 73(5) (5), 609 - 613, English

  [Refereed]

  Scientific journal


• The Effects of Acute Exposure to Deoxynivalenol on Some Inflammatory Parameters in Miniature Pigs

  Osamu Mikami, Midori Kubo, Hideo Murata, Yoshihiro Muneta, Yasuyuki Nakajima, Shigeru Miyazaki, Nobuhiko Tanimura, Ken Katsuda

  May 2011, JOURNAL OF VETERINARY MEDICAL SCIENCE, 73(5) (5), 665 - 671, English

  [Refereed]

  Scientific journal


• Genomic survey of polymorphisms in pattern recognition receptors and their possible relationship to infections in pigs.

  Hirohide Uenishi, Hiroki Shinkai, Takeya Morozumi, Yoshihiro Muneta

  Last, 2011, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 148(1-2) (1-2), 69 - 73

  [Refereed]


• Salivary IgA as a Useful Non-Invasive Marker for Restraint Stress in Pigs

  Yoshihiro Muneta, Tadao Yoshikawa, Yu Minagawa, Tomoyuki Shibahara, Ryuichiro Maeda, Yoshitaka Omata

  Lead, Oct. 2010, JOURNAL OF VETERINARY MEDICAL SCIENCE, 72(10) (10), 1295 - 1300, English

  [Refereed]

  Scientific journal


• Oral vaccination against mycoplasmal pneumonia of swine using a live Erysipelothrix rhusiopathiae vaccine strain as a vector.

  Ogawa Y, Oishi E, Muneta Y, Sano A, Hikono H, Shibahara T, Yagi Y, Shimoji Y

  33, Jul. 2009, Vaccine, 27(33) (33), 4543 - 4550

  [Refereed]

  Scientific journal


• Bovine anterior pituitary progenitor cell line expressing IL-18 and IL-18 receptor.

  Yasuhiro Nagai, Hideki Ogasawara, Yoshikazu Taketa, Hisashi Aso, Takashi, Kanaya, Kouichi Watanabe, Shuichi Ohwada, Yoshihiro Muneta, Takahiro, Yamaguchi

  Nov. 2008, J. Neuroendcrinol., 20(11) (11), 1233 - 1241

  [Refereed]


• Immune response of gnotobiotic piglets against Mycoplasma hyopneumoniae.

  Muneta Y, Minagawa Y, Shimoji Y, Ogawa Y, Hikono H, Mori Y

  Lead, Japanese Society of Veterinary Science, Oct. 2008, The Journal of veterinary medical science, 70(10) (10), 1065 - 1070

  [Refereed]

  Scientific journal


• Expression of inflammatory-related factors in porcine anterior pituitary cell line.

  Yasuhiro Nagai, Hideki Ogasawara, Yoshikazu Taketa, Hisashi Aso, Takashi Kanaya, Masato Miyake, Kouichi Watanabe, Shuichi Ohwada, Yoshihiro, Muneta, Takahiro Yamaaguchi

  Mar. 2008, Vet Immunol. Immunopathol., 123(3-4) (3-4), 201 - 208

  [Refereed]


• Development and immunogenicity of recombinant Mycoplasma gallisepticum vaccine strain ts-11 expressing chicken IFN-γ.

  Yoshihiro Muneta, Indu Somasundra Panicker, Anna Kanci, Douglas Craick, Amir Noormohamimadi, Andrew Bean, Glenn Browning

  Lead, 2008, Vaccine, 26(43) (43), 5449 - 5454

  [Refereed]


• Susceptibility of germ-free pigs to challenge with protease mutants of Salmonella enterica serotype Typhimurium.

  Igor Splichal, Ivan Rychlik, Daniela Gregorova, Alena Sebkoba, Ilja, Trebichavsky, Alla Splichalova, Yoshihiro Muneta, Yasuyuki Mori

  Jul. 2007, Immunobiology, 212(7) (7), 577 - 582

  [Refereed]


• IL-18 expression in pigs following infection with Mycoplasma hyopneumoniae.

  Muneta Y, Minagawa Y, Shimoji Y, Nagata R, Markham PF, Browning GF, Mori Y

  Lead, Mary Ann Liebert Inc, Sep. 2006, Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, 26(9) (9), 637 - 644

  [Refereed]

  Scientific journal


• Immunosuppression of xenograft rejection in porcine kidney PK15 cells by porcine IL-18.

  Yun Sil Choi, Young Kwan Kim, Jung Hyun Shim, Enu Mi Kim, Hyung Sik, Kang, Do Young yoon, Yoshihiro Muneta, Pyung Keun Myung

  May 2006, Exp. Mol. Med., 38(5) (5), 574 - 582

  [Refereed]


• Biased distribution of single nucleotide polymorphisms (SNPs) in porcine Toll-like receptor 1 (TLR1), TLR2, TLR4, TLR5, and TLR6 genes.

  Shinkai H, Tanaka M, Morozumi T, Eguchi-Ogawa T, Okumura N, Muneta Y, Awata T, Uenishi H

  4, May 2006, Immunogenetics, 58, 324 - 330

  [Refereed]


• Porcine Toll-like receptor 1, 6, and 10 genes: complete sequencing of genomic region and expression analysis.

  Shinkai H, Muneta Y, Suzuki K, Eguchi-Ogawa T, Awata T, Uenishi H

  9, Mar. 2006, Molecular immunology, 43, 1474 - 1480

  [Refereed]


• Cellular localization of IL-18 and IL-18 receptor in pig anterior pituitary gland.

  Yasuhiro Nagai, Kouichi Watanabe, Hisashi Aso, Shyuichi Ohwada, Yoshihiro, Muneta, Takahiro Yamaguchi

  Feb. 2006, Domest. Anim. Endcrinol., 30(2) (2), 144 - 154

  [Refereed]


• Attenuated aroA Salmonella enterica serovar typhimurium dose not induce inflammatory response and early protection of gnotobiotic pigs against parental virulent LT2 strain.

  Ilja Trebichavsky, Ivan Rychlick, Alla Splichalova, Hana Hojna, Yoshihiro, Muneta, Yasuyuki Mori, Igor Splichal

  2006, Vaccine, 24(20) (20), 4285 - 4289

  [Refereed]


• Expression cloning of gamma interferon-inducing antigens of Mycobacterium avium subsp paratuberculosis

  R Nagata, Y Muneta, K Yoshihara, Y Yokomizo, Y Mori

  Jun. 2005, INFECTION AND IMMUNITY, 73(6) (6), 3778 - 3782, English

  [Refereed]

  Scientific journal


• Molecular characterization and expression of caprine (Caprahircus) interleukin-18 cDNA.

  Madhusudan Hosamani, B Mondel, Yoshihiro Muneta, TJ Rasool

  May 2005, Int. J. of Immunogenetics, 32(5) (5), 293 - 297

  [Refereed]


• Effect of bacterial virulence on IL-18 expression in the amnion infected with Escherichia coli

  A Splichalova, Trebichavsky, I, Y Muneta, Y Mori, Splichal, I

  May 2005, AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 53(5) (5), 255 - 260, English

  [Refereed]

  Scientific journal


• Genomic structure of eight porcine chemokine receptors and intergene sharing of an exon between CCR1 and XCR1.

  Shinkai H, Morozumi T, Toki D, Eguchi-Ogawa T, Muneta Y, Awata T, Uenishi H

  Apr. 2005, Gene, 349, 55 - 66

  [Refereed]


• The effect of recombinant swine interleukin-4 on swine immune cells and on pro-inflammatory cytokine productions in pigs

  A Nuntaprasert, Y Mori, Y Muneta, K Yoshihara, K Tsukiyama-Kohara, C Kai

  Mar. 2005, COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 28(2) (2), 83 - 101, English

  [Refereed]

  Scientific journal


• Bovine IL-18 ELISA: Detection of IL-18 in sera of pregnant cow and newborn calf, and in colostrum

  Y Muneta, K Yoshihara, Y Minagawa, R Nagata, Y Mori, T Yamaguchi, K Takehara

  Lead, 2005, JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY, 26(3) (3), 203 - 213, English

  [Refereed]

  Scientific journal


• Method for purifying porcine mature interleukin-18 from silkworm haemolymph.

  Hidekazu Nagaya, Yoshihiro Muneta, Chiaki Enomoto, Sayaka Matsumoto, Yuichi Yokomizo, Yasuyuki Mori

  Nov. 2004, Biotechnol. Lett., 26(11) (11), 869 - 873

  [Refereed]


• Generation of multinucleated giant cells in vitro from bovine monocytes and macrophages

  K Yoshihara, R Nagata, Y Muneta, S Inumaru, Y Yokomizo, Y Mori

  Sep. 2004, JOURNAL OF VETERINARY MEDICAL SCIENCE, 66(9) (9), 1065 - 1069, English

  [Refereed]

  Scientific journal


• Expression and one-step purification of bovine interleukin-21 (IL-21) in silkworms using a hybrid baculovirus expression system

  Y Muneta, H Nagaya, Y Minagawa, C Enomoto, S Matsumoto, Y Mori

  Lead, Sep. 2004, BIOTECHNOLOGY LETTERS, 26(18) (18), 1453 - 1458, English

  [Refereed]

  Scientific journal


• Molecular cloning, chromosomal location, and biological activity of porcine interleukin-21

  Y Muneta, R Kikuma, H Uenishi, T Hoshino, K Yoshihara, M Tanaka, NN Hamashima, Y Mori

  Lead, Mar. 2004, JOURNAL OF VETERINARY MEDICAL SCIENCE, 66(3) (3), 269 - 275, English

  [Refereed]

  Scientific journal


• Expression of equine interleukin-18 by baculovirus expression system and its biological activity.

  Donglai Wu, Kenji Murakami, Nihong Liu, Misako Konishi, Yoshihiro Muneta, Shigeki Inumaru, Takehiro Kokuho, Hiroshi Sentsui

  2004, Microbiology and Immunology, 48(6) (6), 471 - 476

  [Refereed]


• Quantitation of bovine macrophage colony-stimulating factor in bovine serum by ELISA

  K Yoshihara, K Oshima, Y Muneta, R Kikuma, C Yayota, T Hirai, N Satoh, S Matsuura, Y Kikyo, M Satoh, C Kubota, S Inumaru, Y Yokomizo, Y Mori

  Oct. 2003, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 95(3-4) (3-4), 103 - 111, English

  [Refereed]

  Scientific journal


• Porcine TLR2 and TLR6: Identification and their involvement in Mycoplasma hyopneumoniae infection

  Y Muneta, H Uenishi, R Kikuma, K Yoshihara, Y Shimoji, R Yamamoto, N Hamashima, Y Yokomizo, Y Mori

  Lead, Oct. 2003, JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 23(10) (10), 583 - 590, English

  [Refereed]

  Scientific journal


• Cloning, expression, and tissue distribution of bovine interleukin-21

  Y Muneta, R Kikuma, K Yoshihara, Y Mori

  Lead, Sep. 2003, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 95(1-2) (1-2), 73 - 80, English

  [Refereed]

  Scientific journal


• Development and application of a pig IL-8 ELISA detection system.

  Igor Splichal, Yoshihiro Munta, Yasuyuki Mori, Eiji Takahashi

  Feb. 2003, J. Immunoassay and Immunochem., 24(2) (2), 219 - 232

  [Refereed]


• Large-scale production of porcine mature interleukin-18 (IL-18) in silkworms using a hybrid baculovirus expression system

  Y Muneta, HK Zhao, S Inumaru, Y Mori

  Lead, Feb. 2003, JOURNAL OF VETERINARY MEDICAL SCIENCE, 65(2) (2), 219 - 223, English

  [Refereed]

  Scientific journal


• Systemic and local cytokine response of young piglets to oral infection with Salmonella enterica serotype typhimurium

  Trebichavsky, I, Spichal, I, A Splichalova, Y Muneta, Y Mori

  2003, FOLIA MICROBIOLOGICA, 48(3) (3), 403 - 407, English

  [Refereed]

  Scientific journal


• Vaccine efficacy of the attenuated Erysipelothrix rhusiopathiae YS-19 expressing a recombinant protein of Mycoplasma hyopneumoniae P97 adhesin against mycoplasmal pneumonia of swine

  Y Shimoji, E Oishi, Y Muneta, H Nosaka, Y Mori

  Jan. 2003, VACCINE, 21(5-6) (5-6), 532 - 537, English

  [Refereed]

  Scientific journal


• Molecular cloning and chromosomal assignment to SSC12p13-->p11 of swine chemokine receptor CCR7.

  Shinkai H, Muneta Y, Eguchi T, Suzuki K, Awata T, Uenishi H

  2, 2003, Cytogenetic and genome research, 101, 155 - 160

  [Refereed]


• Expression of proinflammatory cytokine mRNA in the lymphatic organs of adult and neonatal pigs

  O Mikami, Y Muneta, Y Mori, Y Yokomizo, Y Nakajima

  Dec. 2002, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 90(3-4) (3-4), 203 - 207, English

  [Refereed]

  Scientific journal


• Cloning, expression analyses, and chromosomal location of the porcine interleukin-18 receptor alpha chain (IL-18R alpha)

  Y Muneta, H Uenishi, R Yamamoto, K Yoshihara, H Yasue, T Awata, Y Mori

  Lead, Sep. 2002, JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 22(9) (9), 995 - 1002, English

  [Refereed]

  Scientific journal


• Expression of interleukin-18 by porcine airway and intestinal epithelium

  Y Muneta, N Goji, NM Tsuji, O Mikami, Y Shimoji, Y Nakajima, Y Yokomizo, Y Mori

  Lead, Aug. 2002, JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 22(8) (8), 883 - 889, English

  [Refereed]

  Scientific journal


• Early cytokine response of gnotobiotic piglets to Salmonella enterica serotype Typhimurium

  Splichal, I, Trebichavsky, I, Y Muneta, Y Mori

  May 2002, VETERINARY RESEARCH, 33(3) (3), 291 - 297, English

  [Refereed]

  Scientific journal


• High level expression and purification of bioactive bovine interleukin-18 using a baculovirus system.

  Tomoshi Nagata, Satoko Ishikawa, Eiko Shimokawa, Makino Kamiyama, Reiko, Kikuma, Yoshihiro Muneta, Yuichi Yokomizo, Masayuki Nakamura, Kazuaki Takehara

  2002, Vet. Immunol. Immunopathol., 87(1-2) (1-2), 65 - 72

  [Refereed]


• Erysipelothrix rhusiopathiae YS-1 as a live vaccine vehicle for heterologous protein expression and intranasal immunization of pigs.

  Shimoji Y, Oishi E, Kitajima T, Muneta Y, Shimizu S, Mori Y

  We have developed a system in which a foreign antigen is delivered and expressed on the surface of an attenuated strain of Erysipelothrix rhusiopathiae YS-1 and have examined the ability of a such recombinant E. rhusiopathiae strain to function as a mucosal vaccine vector. The C-terminal portion, including two repeat regions, R1 and R2, of the P97 adhesin of Mycoplasma hyopneumoniae strain E-1 was successfully translocated and expressed on the E. rhusiopathiae YS-1 cell surface after it was fused to SpaA.1, a cell surface protective antigen of E. rhusiopathiae. BALB/c mice subcutaneously immunized with the E. rhusiopathiae recombinant strains developed specific antibodies against SpaA.1 protein and were protected from lethal challenge with the highly virulent homologous E. rhusiopathiae Fujisawa-SmR strain, showing the efficacy of this heterologous-antigen expression system as a vaccine against E. rhusiopathiae infection. To determine whether protective immune responses are induced in target species, newborn, specific-pathogen-free piglets were immunized intranasally with a recombinant strain designated YS-19. The immunized piglets developed specific anti-SpaA.1 immunoglobulin G (IgG) antibodies in their serum and were protected from death by erysipelas, showing that mucosal vaccination of piglets with YS-19 induces systemic immune responses. Furthermore, YS-19-immunized piglets showed higher levels of P97-specific IgA antibodies in the respiratory tract than did YS-1-immunized piglets. Thus, E. rhusiopathiae YS-1 appears to be a promising vaccine vector for mucosal delivery that can induce local and systemic immune responses.

  American Society for Microbiology, Jan. 2002, Infection and immunity, 70(1) (1), 226 - 232

  [Refereed]

  Scientific journal


• Assignment of swine TNERSF6(Fas) to chromosome 14q25-->q27 and TNFSF6(FasL) to chromosome 9q14-->q22 by FISH and radiation hybrid mapping.

  Yamamoto R, Muneta Y, Hiraiwa H, Kiuchi S, Yasue H, Takagaki Y, Awata T, Uenishi H

  2002, Cytogenet Genome Res, 97(3-4) (3-4), 276D


• Porcine caspase-3: Its cloning and activity during apoptosis of PK15 cells induced by porcine Fas ligand

  Y Muneta, Y Shimoji, Y Mori

  Lead, Jun. 2001, JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 21(6) (6), 409 - 415, English

  [Refereed]

  Scientific journal


• Molecular cloning, characterization, and expression of porcine fas ligand (CD95 ligand)

  Y Muneta, Y Shimoji, S Inumaru, Y Mori

  Lead, May 2001, JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 21(5) (5), 305 - 312, English

  [Refereed]

  Scientific journal


• Efficient production of biologically active porcine interleukin-18 by coexpression with porcine caspase-1 using a baculovirus expression system

  Y Muneta, S Inumaru, Y Shimoji, Y Mori

  Lead, Feb. 2001, JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 21(2) (2), 125 - 130, English

  [Refereed]

  Scientific journal


• Porcine interleukin 18: Cloning, characterization of the cDNA and expression with the baculovirus system

  Y Muneta, Y Mori, Y Shimoji, Y Yokomizo

  Lead, Jun. 2000, CYTOKINE, 12(6) (6), 566 - 572, English

  [Refereed]

  Scientific journal


• Detection of porcine interleukin-18 by sandwich ELISA and immunohistochemical staining using its monoclonal antibodies

  Y Muneta, O Mikami, Y Shimoji, Y Nakajima, Y Yokomizo, Y Mori

  Lead, Mar. 2000, JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 20(3) (3), 331 - 336, English

  [Refereed]

  Scientific journal


• Production of monoclonal antibodies to porcine interleukin-18 and their use for immunoaffinity purfication of recombinant porcine interleukin-18

  Y Muneta, Y Shimoji, Y Yokomizo, Y Mori

  Lead, Mar. 2000, JOURNAL OF IMMUNOLOGICAL METHODS, 236(1-2) (1-2), 99 - 104, English

  [Refereed]

  Scientific journal


• Molecular cloning of porcine interleukin-1 beta converting enzyme and differential gene expression of IL-1 beta converting enzyme, IL-1 beta, and IL-18 in porcine alveolar macrophages

  Y Muneta, Y Shimoji, Y Yokomizo, Y Mori

  Lead, Nov. 1999, JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 19(11) (11), 1289 - 1296, English

  [Refereed]

  Scientific journal


• Expression of LacZ gene controlled by various promoters in mouse preimplantation embryos.

  Yoshihiro Muneta, Naoko Kimura, Toshiyuki Kudo, Masaya Yamanaka, Yoshiaki Itagaki

  Lead, 1998, J. Mamm Ova Res, 15, 81 - 86

  [Refereed]

• 豚における細菌叢の有無によるA群ロタウイルス感染への応答の差異

  須田 遊人, 生井 楓, 宗田 吉広, 西川 明芳, 平松 香菜恵, 大橋 誠一, 岸田 なつみ, 今村 圭哉, 犬童 優樹, 西山 啓太, 北澤 春樹, 宮﨑 綾子

  Sep. 2025, 第168回日本獣医学会講演要旨


• TLR3を介したTLR4発現調節によるウイルス・細胞複合感染時の炎症応答増強機構の解析

  生井 楓, 小林 稔, 今村圭哉, 鈴木香澄, 吉岡 豪, 須田遊人, 宗田吉広, 上西博英, 西山啓太, 北澤春樹

  Sep. 2025, 第133回日本畜産学講演要旨集


• ワカメ残渣が子豚の腸内細菌叢及び代謝に与える影響

  小澤 陸, 西山啓太, 扇 隆介, 龔 緯辰, 生井 楓, 今井勇志, 宗田吉広, 須田義人, 北澤春樹

  Sep. 2025, 第133回日本畜産学講演要旨集


• 牛ルーメンMFCs由来の新規Methyloceanibacter属菌のシングルセルゲノム解析

  宗田吉広, 小川洋介, 西村祐貴, 澤田 浩, 山本道貴, 伊藤寿浩, 新井鐘蔵

  Sep. 2025, 第133回日本畜産学講演要旨集


• ワカメ未利用部位とワカメ資化性イムノバイオティクス乳酸菌の離乳子豚への給与によるイムノシンバイオティクス効果の検証

  宗田 吉広, 生井 楓, 須田義人, 今井 勇志, 小林朋生, 曽地雄一郎, 西山啓太, 上本吉伸, 新開浩樹, 北澤春樹

  Jul. 2025, 令和7年度日本乳酸菌学会講演要旨


• カシュ―ナッツ殻液（CNSL）投与牛におけるルーメン液のNMRメタボロミクスよる代謝産物の解析

  鈴木桃香, 宗田吉広, 関山恭代, 澤田浩, 小林大樹, 疋田千枝, 新井鐘蔵

  Jan. 2025, 第42回日本獣医医師会獣医学術学会年次大会講演要旨


• 銅抵抗性サルモネラ感染試験における腸内細菌叢,盲腸内代謝産物及び血清脂質代謝マーカーの解析

  宗田吉広, 西浦玲奈, 新井暢夫, 関山恭代, 澤田浩, 須田智子, 芝原友幸

  2023, 日本獣医学会学術集会講演要旨集, 166th


• サルモネラの腸管定着における非定型Salmonella Typhimurium流行系統が持つ可動性遺伝因子の役割

  新井暢夫, 西浦玲奈, 芝原友幸, 芝原友幸, 玉村雪乃, 渡部綾子, 岩田剛敏, 宗田吉広, 秋庭正人, 楠本正博, 楠本正博

  2023, 日本獣医学会学術集会講演要旨集, 166th


• 銅抵抗性を有するSalmonella Typhimuriumの感染による銅給与下の豚体内の微量元素濃度および炎症マーカーの変動

  西浦玲奈, 新井暢夫, 宗田吉広, 澤田浩, 西浦颯, 芝原友幸

  2023, 日本獣医学会学術集会講演要旨集, 166th


• 子豚の離乳時体重の高低による血液生化学性状値及び腸内細菌叢の比較

  鈴木 香澄, 吉岡 豪, 須田 智子, 新開 浩樹, 上西 博英, 北澤 春樹, 宗田 吉広

  (公社)日本畜産学会, Sep. 2022, 日本畜産学会大会講演要旨集, 130回, 117 - 117, Japanese


• 竹炭電極内にルーメン細菌叢を定着させることによるルーメン細菌叢発電の効率化

  宗田吉広, 矢代雄介, 澤田浩, 芝原友幸, 須田智子, 西浦玲奈, 伊藤寿浩, 新井鐘蔵

  2022, 日本獣医学会学術集会講演要旨集, 165th (CD-ROM)


• 牛のルーメン内容物を材料とした微生物燃料電池により作製した微酸性次亜塩素酸水の消毒効果の検討について

  松澤直樹, 塩原綾早, 中村南斗, 西森朝美, 澤田浩, 高松大輔, 岡本真理子, 小川洋介, 江口正浩, 宗田吉広

  2022, 日本産業動物獣医学会(中部)・日本小動物獣医学会(中部)・日本獣医公衆衛生学会(中部), 2022


• Recent topics and studies on the relationship of porcine antibiotic resistant bacteria with gut microbiota-Toward the establishment of sustainable pig industry-

  宗田吉広, 新開浩樹, 廣瀬健右, 上西博英, 北澤春樹

  Lead, Aug. 2021, 日本豚病研究会報, (78) (78), 28 - 36, Japanese

  [Refereed]

  Introduction scientific journal


• 牛のルーメンセンサとルーメン内細菌叢に関する最近の話題と研究

  宗田吉広, 澤田 浩, 岡田浩尚, 伊藤寿浩, 山中典子, 新井鐘蔵

  Lead, Feb. 2021, 日本獣医師雑誌, 74(2) (2), 121 - 126, Japanese

  [Refereed]

  Introduction scientific journal


• パターン認識受容体遺伝子多型と豚の産肉形質の関連

  廣瀬健右, 宗田吉広, 伊藤哲也, 上川舞, 東間千芽, 古川了一, 二階堂聡, 松本敏美, 上西博英

  2021, 日本畜産学会大会講演要旨, 129th


• 農業・獣医畜産業の視点から新型コロナウイルス感染症（COVID-19）を考える

  宗田 吉広

  Lead, Dec. 2020, 動物用ワクチン・バイオ医薬品研究会ニュースレター, 22, 3 - 6


• 豚におけるプロバイオティクスおよび腸内細菌叢に関する最近の話題と研究

  宗田吉広

  Lead, Aug. 2020, 日本豚病研究会報, (76) (76), 15 - 23, Japanese

  [Refereed]

  Introduction scientific journal


• 豚の唾液中ストレスマーカーとアニマルウェルフェアに関する最近の話題と研究

  宗田吉広

  Lead, Aug. 2019, 日本豚病研究会報, (74) (74), 34 - 41, Japanese

  [Refereed]

  Introduction scientific journal


• 豚のToll-like receptorsとその一塩基多型及び疾病との関連に関する研究

  宗田 吉広, 新開 浩樹, 上西 博英

  Lead, 日本豚病研究会, Aug. 2018, 日本豚病研究会報, 72(72) (72), 24 - 30, Japanese

  [Refereed]

  Introduction scientific journal


• サルモネラ認識を低下させる豚TLR5の一塩基多型とその豚サルモネラ症に及ぼす影響の検証

  宗田吉広, 新井暢夫, 新井暢夫, 矢ヶ部陽子, 新開浩樹, 芝原友幸, 江口正浩, 上西博英, 秋庭正人

  (公社)日本獣医学会, Aug. 2018, 日本獣医学会学術集会講演要旨集, 161回, 204 - 204, Japanese


• サルモネラ認識を低下させる豚TLR5-塩基多型(C1205T)に関する研究 SNPの発見から、機能解析、遺伝子型別、頻度解析および実験感染による検証まで

  宗田 吉広, 新井 暢夫, 矢ヶ部 陽子, 江口 正浩, 芝原 友幸, 佐久間 晶子, 新開 浩樹, 上西 博英, 廣瀬 健右, 秋庭 正人

  日本豚病研究会, Aug. 2017, 日本豚病研究会報, 70(70) (70), 25 - 31, Japanese

  [Refereed]

  Introduction scientific journal


• 栃木県内の1養豚場における衛生対策指導前後での病原体浸潤状況と生産性指標及び血清中ストレスマーカー濃度の変動

  藤田慶一郎, 市川優, 新楽和孝, 矢ヶ部陽子, 菊佳男, 宗田吉広

  2016, 日本獣医師会獣医学術学会年次大会講演要旨集, 2015 (CD-ROM)


• 肥育豚において群編成後の移動及びワクチン接種が各種ストレス指標や抗体応答及び増体に与える影響

  藤田慶一郎, 菊佳男, 溝呂木聖子, 矢ケ部陽子, 宗田吉広

  30 Jan. 2015, 日本獣医師会獣医学術学会年次大会講演要旨集, 2014, 227, Japanese


• Effects of Crowding on Weight Gain, Immune Performance and the Concentration of Salivary Stress Markers in Growing-Finishing Pigs

  FUJITA Keiichiro, KIKU Yoshio, TAKAHASHI Takashi, NOGUCHI Munehiko, MIZOROGI Seiko, YAKABE Yoko, MUNETA Yoshihiro

  This study was carried out to determine the effects of crowding on weight gain, immune performance and the concentration of salivary stress markers in growing-finishing pigs. Space allowances were 0.37 m²/pig (crowding stress group, n＝14) and 0.87 m²/pig (control group, n＝6) in a fully slatted facility (5.24 m²). Initial weights averaged 52.3 kg, and the pigs were tested for 14 days. The average daily gains were significantly lower in the crowding stress group than in the control group on days 0-7 (P＜0.05) and days 7-14 (P＜0.01). The lymphocyte proliferation indexes in response to Concanavalin A were significantly higher in the crowding stress group than in the control group on day 14. The CD4:CD8 ratio was significantly lower in the crowding stress group than in the control group on day 14. CD8＋ and CD14＋ lymphocytes were significantly higher (P＜0.05) in the crowding stress group than in the control group on day 14. Salivary stress markers (Cortisol, IL-18 and IgA) did not change as a result of crowding stress. These results suggest that crowding stress in growing-finishing pigs can have a negative impact on weight gain and alter immune function.

  Japan Veterinary Medical Association, 2015, Journal of the Japan Veterinary Medical Association, 68(1) (1), 43 - 47, Japanese

  [Refereed]


• Experimental infection of Salmonella enterica serovar Choleraesuis to HPCD piglets and trial to validate the effect of a TLR5 SNP (C1205T)

  Mizorogi, S, Yakabe, Y, Shibahara, T, Akiba, M, Kusumoto, M, Sakuma, A, Nakajo, M, Shinkai, H, Uenishi,H, Muneta, Y

  Feb. 2014, Proceedings of the Japanese Pig Veterinary Society, (63) (63), 6 - 11

  [Refereed]


• HPCD子豚を用いたSalmonella Choleraesuis感染実験におけるTLR5 SNP(C1205T)の影響の検証

  溝呂木聖子, 溝呂木聖子, 矢ケ部陽子, 芝原友幸, 三好洋嗣, 秋庭正人, 秋庭正人, 楠本正博, 佐久間晶子, 中条満, 新開浩樹, 上西博英, 宗田吉広, 宗田吉広

  (公社)日本獣医学会, Aug. 2013, 日本獣医学会学術集会講演要旨集, 156回, 252 - 252, Japanese


• Salmonella Choleraesuisの離乳子豚に対する感染試験:TLR5の一塩基多型(C1205T)の影響

  宗田吉広, 宗田吉広, 溝呂木聖子, 溝呂木聖子, 矢ケ部陽子, 芝原友幸, 秋庭正人, 楠本正博, 佐久間晶子, 中條満, 新開浩樹, 上西博英

  (公社)日本獣医学会, Aug. 2013, 日本獣医学会学術集会講演要旨集, 156回, 252 - 252, Japanese


• Host immune responses against Mycoplasma infection in domestic animal species

  MUNETA Yoshihiro

  31 Mar. 2013, 日本マイコプラズマ学会雑誌 = Japanese Journal of Mycoplasmology, 39, 28 - 28, Japanese


• ブタ抗病性,肉質等経済形質向上のための育種技術の開発 第1章 抗病性育種のための家畜免疫系遺伝子解析とブタ実験家系及び実用集団での評価

  上西博英, 小川智子, 新開浩樹, 安藤麻子, 渡辺智正, 鈴木啓一, 小林秀樹, 芝原友幸, 鈴木孝子, 宗田吉広, 国保健浩, 森松文毅, 村上博, 高萩陽一, 助川慎, 鈴木英作, 門脇宏, 柴田千尋, 中條満, 佐久間晶子

  15 Mar. 2013, 農林水産省農林水産技術会議事務局研究成果, (496) (496), 35 - 42, Japanese


• 離乳子豚に対する畜舎内空気環境の改善とプロバイオティック製剤の投与が生産性に与える効果

  山根逸郎, 佐々木羊介, 宗田吉広, 池口厚男, 中久保亮, 国保健浩

  Respiratory syndromes and diarrhea among weaned pigs decrease number of marketed pigs and reduce growth performance. This clinical trial was conducted to evaluate the effects of environmental improvements and probiotic supplement on mortality, body weight gain and saliva cortisol levels among weaned pigs reared in a farm, which known to have adversed sanitary conditions. In October 2010, 140 pigs weaned in this month were classified into 4 body weight groups (heavy, medium, light, extreme light) (n=35 each). Each group was further randomly allocated into 4 groups such as reared in; A) cleaned room with probiotics (Lactobachillus casei I-5 (1%)), B) cleaned room without probiotics, C) uncleaned room with probiotics, D) uncleaned room without probiotics. Therefore, 16 pens (8-10 animals each) were allocated into 4 treatment groups (A to D) and 4 blocks by their weights. Body weights of the targeted animals were measured weekly until 9 weeks and saliva obtained at 0, 1, 4 and 8 weeks post weaning were used for cortisol measurement. In extreme light weight group, pigs in cleaned room and those with probiotics had significantly higher body weight gain compared to other groups. In the same weight group, pigs without probiotics had higher mortality compared to other group and this was more evident among castrated male group. At 4 weeks post weaning, cortisol concentration in saliva remained highest levels among castrated male in extreme light weight group without probiotics compared to other groups. From this study, cleaning process and probiotic supplementation were effective on body weight gain and improvement of mortality only for pigs which were lowest body weight group at weaning. In additions, cortisol concentration in saliva could be a good indicator for the pigs which had deteriorated growth performance.

  The Japan Society of Veterinary Epidemiology, 20 Jul. 2012, 獣医疫学雑誌, 16(1) (1), (JA)25,(EN)26 - 26, Japanese


• Development of allele-specific primer PCR for a swine TLR2 SNP (C406G→P136A) and comparison of the frequency in pigs between Japan and the Czech Republic

  MUNETA Yoshihiro, MINAGAWA Yu, KUSUMOTO Masahiro, SHINKAI Hiroki, UENISHI Hirohide, SPLICHAL Igor

  31 Mar. 2012, 日本マイコプラズマ学会雑誌 = Japanese Journal of Mycoplasmology, 38, 55 - 55, English


• イノシシ属におけるTLR4遺伝子の多型がリポ多糖認識に及ぼす影響の解析

  新開浩樹, 奥村直彦, 鈴木倫太郎, 宗田吉広, 上西博英

  28 Mar. 2012, 日本畜産学会大会講演要旨, 115th, 161, Japanese


• 尾静脈採血法による母豚へのストレス低減効果

  藤田慶一郎, 高橋孝志, 岡崎克美, 齊藤かおり, 溝呂木聖子, 菊佳男, 宗田吉広

  2012, 日本獣医学会学術集会講演要旨集, 154th


• サルモネラ認識能が低下するTLR5一塩基多型(C1205T)の簡易検出法

  宗田吉広, 皆川遊, 楠本正博, 新開浩樹, 上西博英

  2012, 農研機構動物衛生研究所成果情報(Web), 2012


• DEVELOPMENT OF ALLELE SPECIFIC PRIMER PCR FOR A SWINE TLR5 SNP (C1205T-P402L) AND COMPARISON OF THE FREQUENCY IN PIGS BETWEEN JAPAN AND THE CZECH REPUBLIC

  MUNETA Yoshihiro, MINAGAWA Yu, KUSUMOTO Masahiro, SHINKAI Hiroki, UENISHI Hirohide, SPLICHAL Igor

  31 Oct. 2011, 日本細菌学雑誌, 66(2-3) (2-3), 365, English


• 唾液中のIgAは豚の非侵襲的ストレスマーカーの一つとなる

  宗田 吉広, 吉川 忠雄, 皆川 遊, 芝原 友幸, 前田 龍一郎, 小俣 吉孝

  2011, 動物衛生研究成果情報, (10) (10), 43 - 44

  [Refereed]


• 死亡事故が多発する養豚農家における環境とストレス指標および経済損失調査

  山根逸郎, 池口厚男, 谷島直樹, 皆川遊, 宗田吉広, 國保健浩

  In 2009, a swine farm which is known to have high mortality of growing pigs was used for this clinical trial. Ear tags were attached to 105 piglets which were borne in June, 2009 and allocated into 2 nursing houses, house A with relatively good environmental conditions and house B with bad sanitary condition with high mortality. The house B had 12 time dust concentrations, 4 times ammonia concentration, 8 times nitrous oxicide and 2 times methane concentration higher than those measured in house A. The IgA concentration in the saliva of the pigs raised in house B was 4 times higher than those in house A. Total mortality after weaning in this trial was 26.2% (16/61) and 40.4% (17/42) for growing pigs raised in house A and B, respectively. This farm has been suffered severe economical losses due to high mortality of growing pigs and proper intervention, particularly in house B, is urgently required.

  The Japan Society of Veterinary Epidemiology, 20 Jul. 2010, 獣医疫学雑誌, 14(1) (1), (JA)23,(EN)24 - 24, Japanese


• 豚の経口投与型ワクチンの開発--豚丹毒と豚マイコプラズマ肺炎をまとめて予防する

  下地 善弘, 小川 洋介, 宗田 吉広

  畜産技術協会, Apr. 2010, 畜産技術, (659) (659), 25 - 29, Japanese


• Strategy for vaccine development using Erysipelothrix rhusiopathiae as a platform vector : oral vaccine for mycoplasmal pneumonia of swine

  OGAWA Yohsuke, OISHI Eiji, MUNETA Yoshihiro, SHIMOJI Yoshihiro

  31 Mar. 2010, 日本マイコプラズマ学会雑誌 = Japanese Journal of Mycoplasmology, 36, 51 - 51, Japanese


• Effect of the allele containing a swine TLR2 SNP (C406G→P136A) on Mycoplasma hyopneumoniae gnotobiotic infection

  MUNETA Yoshihiro, SHINKAI Hiroki, SHIBATA Chihiro, MINAGAWA Yu, OGAWA Yohsuke, SHIBAHARA Tomoyuki, SHIMOJI Yoshihiro, UENISHI Hirohide

  日本マイコプラズマ学会事務局, 31 Mar. 2010, Japanese journal of mycoplasmology, 36(0) (0), 52 - 56, Japanese


• Allele Specific Primer PCRによる豚TLR2一塩基多型の解析と日本とチェコの豚における比較

  宗田吉広, 皆川遊, 楠本正博, 新開浩樹, 上西博英, SPLICHAL Igor

  (公社)日本獣医学会, Mar. 2010, 日本獣医学会学術集会講演要旨集, 149回, 255 - 255, Japanese


• "豚用飲むワクチン"の開発--一つのワクチンで豚丹毒と豚マイコプラズマ肺炎の防御が可能に

  下地 善弘, 小川 洋介, 宗田 吉広

  鶏卵肉情報センター, Jan. 2010, 月刊養豚情報, 38(1) (1), 52 - 55, Japanese


• 豚丹毒および豚マイコプラズマ肺炎をまとめて防御できる"飲むワクチン"の開発

  下地 善弘, 小川 洋介, 宗田 吉広

  日本畜産振興会, Jan. 2010, 養豚の友, (490) (490), 70 - 73, Japanese


• ブタTLR2遺伝子多型とマイコプラズマ肺炎感受性との関連の解析

  新開浩樹, 柴田千尋, 宗田吉広, 門脇宏, 鈴木英作, 皆川遊, 上西博英

  (公社)日本獣医学会, Sep. 2009, 日本獣医学会学術集会講演要旨集, 148回, 214 - 214, Japanese


• Cell-mediated Immunity to Influenza Virus Infections: From the Perspective to the Vaccine Development against Highly Pathogenic Avian Influenza

  Hirokazu Hikono, Masaji Mase, Satoko Watanabe, Yohsuke Ogawa, Yoshihiro Muneta, Takayuki Kubota, Yoshihiro Shimoji

  Oct. 2008, JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY, 42(4) (4), 245 - 249, English

  Introduction scientific journal


• Effect of low-dose oral IL-18 and IL-12 against mycoplasmal pneumonia of swine

  MUNETA Yoshihiro, MINAGAWA Yu, OGAWA Yohsuke, SHIBAHARA Tomoyuki, SHIMOJI Yoshihiro, MORI Yasuyuki

  日本マイコプラズマ学会事務局, 31 May 2008, Japanese journal of mycoplasmology, 35(0) (0), 34 - 37, Japanese


• 豚マイコプラズマ肺炎の発病機構の解明と疾病防除技術への応用－経口低用量IL-18およびIL-12の豚マイコプラズマ肺炎低減化効果の検討

  宗田吉広, 皆川遊, 小川洋介, 芝原友幸, 下地善弘, 森康行

  2008, 日本マイコプラズマ学会雑誌, 35, 36 - 39


• Mycoplasma hyopneumoniae 接種培養気管における遺伝子発現の解析

  森 康行, 永田 礼子, 宗田 吉広

  25 Feb. 2007, 日本細菌学雑誌, 62(1) (1), 143 - 143, Japanese


• Immune responses to the Mycoplasma hyopneumoniae P97 antigen in pigs vaccinated with Erysipelothrix rhusiopathiae YS-19

  下地 善弘, 大石 英司, 宗田 吉広, 芝原 友幸, 森 康行

  Mycoplasma hyopneumoniae P97抗原の一部を菌体表面に発現する豚丹毒菌YS-19株免疫豚のP97抗原に対する免疫応答の解析を行った。YS-19株を3回経鼻的に免疫された豚では，P97抗原に対する血中抗体は検出されなかったが，末梢血単核球は3回目の免疫直後からP97抗原刺激に対して増殖反応を示し，その程度は対照豚と比べて有意な差が認められた。また，M. hyopneumoniae強毒株攻撃接種後に採取した末梢血単核球はP97抗原刺激に対して細胞性免疫の重要な指標であるIFN-γを産生した。免疫，非免疫に拘わらず，豚の末梢血単核球はP97抗原の刺激によりIL-8を産生し，P97抗原を鼻腔内に噴霧投与された豚では気管支肺胞洗浄液中に多量の好中球が観察された。さらに，YS-19株のNeedle-free injector(針なし注射)を用いたワクチン接種法の検討において，皮内に1回免疫した豚の末梢血単核球は，M. hyopneumoniae強毒株攻撃接種後にP97抗原刺激による増殖能が有意に上昇した。なお，Needle-free injectorによるYS-19株免疫豚では肺炎病変の形成が有意に抑えられた。

  農業技術研究機構動物衛生研究所, 2007, Bulletin of the National Institute of Animal Health, (113) (113), 1 - 11, Japanese

  [Refereed]


• 豚丹毒・豚マイコプラズマ肺炎多価ワクチンの針なし注射による接種

  下地 善弘, 大石 英司, 宗田 吉広, 芝原 友幸, 森 康行

  2007, 動物衛生研究成果情報, (6) (6), 21 - 22

  [Refereed]


• カイコとバキュロウイルスを用いた家畜用サイトカインの生産と今後の展望

  宮澤 光博, 神村 学, 倉田 啓而, 犬丸 茂樹, 宗田 吉広, 國保 健浩, 津田 英利

  社団法人 日本蚕糸学会, Aug. 2006, 蚕糸・昆虫バイオテック, 75(2) (2), 103 - 109, Japanese

  [Refereed]


• ブタTLR遺伝子のSNP検索とその発現の解析

  新開浩樹, 田中麻衣子, 両角岳哉, 小川智子, 奥村直彦, 鈴木恒平, 宗田吉広, 粟田崇, 上西博英

  (公社)日本畜産学会, Aug. 2005, 日本畜産学会大会講演要旨, 105回, 61 - 61, Japanese


• ブタTLR2およびTLR6のクローニングとMycoplasma hyopneumoniae自然免疫応答への関与

  宗田吉広, 上西博英, 菊間礼子, 吉原一浩, 下地善弘, 山本竜司, 浜島紀之, 横溝祐一, 森康行

  29 Oct. 2004, 動物衛生研究成果情報, (3) (3), 27 - 28, Japanese


• Interleukin-18 expression in pigs infected with Mycoplasma hyopneumoniae

  MUNETA Yoshihiro, MINAGAWA Yu, NAGATA Reiko, YOSHIHARA Kazuhiro, SHIMOJI Yoshihiro, MORI Yasuyuki

  30 Sep. 2004, 日本マイコプラズマ学会雑誌 = Japanese Journal of Mycoplasmology, 31, 7 - 8, English


• ブタCCケモカインレセプター遺伝子群のゲノム構造とその発現の解析

  新開浩樹, 両角岳哉, 土岐大輔, 江口智子, 宗田吉広, 粟田崇, 上西博英

  25 Nov. 2003, 日本分子生物学会年会プログラム・講演要旨集, 26th, 795, Japanese


• ブタTLR2とTLR6の同定とMycoplasma hyopneumoniae感染における関与

  宗田吉広, 上西博英, 菊間礼子, 吉原一浩, 下地善弘, 山本竜司, 浜島紀之, 横溝祐一, 森康行

  (公社)日本獣医学会, Sep. 2003, 日本獣医学会学術集会講演要旨集, 136回, 157 - 157, Japanese


• ブタIL‐21のクローニングと発現およびマッピング

  菊間礼子, 宗田吉広, 上西博英, 山本竜司, 田中麻衣子, 浜島紀之, 粟田崇, 吉原一浩, 森康行

  (公社)日本獣医学会, Mar. 2003, 日本獣医学会学術集会講演要旨集, 135回, 112 - 112, Japanese


• ブタ胸腺及び末梢血リンパ球cDNAライブラリーのESTデータベース構築による免疫系分子の網羅的同定

  上西博英, 鈴木恒平, 沢崎哲哉, 土岐大輔, 新開浩樹, 宇田吉広, 浜島紀之, 粟田崇

  (公社)日本獣医学会, Mar. 2003, 日本獣医学会学術集会講演要旨集, 135回, 112 - 112, Japanese


• 豚インターロイキン18レセプターα鎖のクローニングと発現解析

  宗田吉広, 山本竜司, 上西博英, 吉原一浩, 森康行

  (公社)日本獣医学会, Mar. 2002, 日本獣医学会学術集会講演要旨集, 133回, 97 - 97, Japanese


• ヨーネ病の診断法に関する研究

  森 康行, 菊間 礼子, 宗田 吉広

  ヨーネ病診断技術の改良，開発を目的として，1)PCRによる糞便中ヨーネ菌の検出，2)インターフェロン・ガンマ(IFN-γ)検出によるヨーネ病の早期診断，3)ヨーネ菌特異抗原及び表面抗原の解析と診断への応用，4)ヨーネ菌感染における細胞性免疫応答の解析等，様々な角度からヨーネ病の新たな診断法について検討した。PCR法として，internal control DNAを添加した反応系が，サンプル毎の糞便由来PCR反応阻害作用の影響を検定することが可能であり，糞便中ヨーネ菌検出法として有用であった。IFN-γ検査では，陽性対照として設けたconcanavalin A(Con A)刺激によるIFN-γの産生量が，健康牛では最も高い値を示すのに対し，ヨーネ病感染牛ではヨーニンPPD刺激時のIFN-γ産生量が最も高い傾向が認められたことから，この点を診断上の一つの基準とすることが望ましい。ヨーネ菌の抗原解析において，類似の鳥型結核菌とは反応しないヨーネ菌に対して特異性の高いモノクローナル抗体(Mab)が得られた。さらに，当該Mabはヨーネ菌の表面に存在するリポアラビノマンナンを認識していた。ヨーネ菌Alkyl hydroperoxide reductases Cの遺伝子組換え体を作出し，本組換え抗原がヨーネ菌実験感染牛の血液細胞からIFN-γの産生を誘導することを確認した。ケモカインの一つであるmonocyte chemoattractant protein-1の産生とヨーネ菌感染との関連がマウス感染モデル実験において示唆された。

  農業技術研究機構動物衛生研究所, 2002, 動物衛生研究所研究報告, (109) (109), 33 - 42, Japanese


• Development of a new vaccine for the millennium : use of E. rhusiopathiae mutant strain as a vaccine vector

  SHIMOJI Yosihiro, MORI Yasuyuki, SEKIZAKI Tsutomu, ARAI Keigo, MUNETA Yoshihiro, YOKOMIZO Yuuichi

  農林水産技術情報協会, 01 Mar. 1999, 農林水産技術研究ジャーナル, 22(3) (3), 38 - 42, Japanese


• Future prospects of next generation of erysipelas vaccine

  SHIMOJI Y., MUNETA Y., SEKIZAKI T., YOKOMIZO Y.

  01 Feb. 1999, 日本豚病研究会報 = Proceedings of the Japanese Pig Veterinary Society, (34) (34), 8 - 11, Japanese


• Detection of Mycoplasma dispar from pneumonic lungs of beef cattle

  Mori Yasuyuki, NISHIMOTO S., OMOTO T., MUNETA Y., SHIMOJI Y., KOBAYASHI H.

  30 Dec. 1998, 日本マイコプラズマ学会雑誌 = Japanese Journal of Mycoplasmology, 25, 78 - 80, Japanese

• 養豚場の呼吸器疾病対策

  宗田吉広

  Contributor, 豚マイコプラズマ肺炎, 緑書房, Jul. 2017


• 動物用ワクチンとバイオ医薬品 : 新たな潮流

  動物用ワクチン・バイオ医薬品研究会

  ワクチンアジュバントとワクチン投与方法の現状と研究開発, 文永堂出版, Jul. 2017, Japanese, ISBN: 9784830032677


• 最新マイコプラズマ学

  宗田 吉広

  Contributor, 動物マイコプラズマの免疫応答, 近代出版, Jan. 2016


• 動物用ワクチンーその理論と実際ー

  Contributor, これからのワクチンデリバリーとアジュバント, 文永堂出版, Oct. 2011


• 最新農業技術辞典（Naropedia）

  Contributor, イムノブロット法、ケモカイン、Th1/Th2、ノトバイオ―ト、分化・成熟、無菌動物, 社団法人農山漁村文化協会, Mar. 2006

• Germ-free piglet model of Immunocompromised preterm infants.

  Igor Splichal, Alla Splichalova, Sharon M. Donovan, Katerina Polakova, Zdislava Kindlova, Vera Neuzil Bunesova, Nikol Kadlec Modrackova, Eva Vlkova, Yoshihiro Muneta

  8th International Conference on Model Hosts., English


• 肥育豚の増体と関連する腸内細菌叢および代謝物の探索と機能解析

  扇隆介, 襲緯辰, 生井楓, 渡邊元春, 鈴木香澄, 吉岡豪, 宗田吉広, 有吉理, 岡健太郎, 高橋志達, 北澤春樹, 西山啓太

  第134回日本畜産学会, Japanese

  Oral presentation


• Haptoglobin as a potential biomarker for inflammation in Hysterotomy-produced colostrum-deprived piglets transplated with porcine fecal microbiota.

  Yoshihiro Muneta, Yuto Suda, Sayaka Nishikawa, Akari Tachibana, Kanae Hiramatsu, Keigo Ikeda, Masahiro Eguchi, Yoshifumi Iwamaru, Ayako Miyazaki, Yuki Indo, Fu Namai, Haruki Kitazawa

  World Veterinary Association Congress 2026, English

  Poster presentation


• カシューナッツ殻液（CNSL）給与による牛ルーメン内細菌叢の短期的変遷および長期的影響の検討

  小林大樹, 宗田吉広, 澤田 浩, 新井鐘蔵

  日本農芸化学会2026年度大会, Mar. 2026

  Oral presentation


• 牛ルーメンMFCsのシングルセルゲノム解析による新規Methyloceanibacter属菌ゲノムの同定

  宗田吉広, 小川洋介, 西村祐貴, 山本道貴, 伊藤寿浩, 澤田浩, 新井鐘蔵

  2025年度先進ゲノム支援拡大班会議

  Poster presentation


• Evaluation of immotalized porcine macrophages cell lines for improved progression of PRRSV-1 and PRRSV-2 isolates.

  Ayako Miyazaki, Natsumi Kishida, Michihiro Takagi, Yuto Suda, Seiichi Ohashi, Yoshihiro Muneta, Kasumi Suzuki, Go Yoshioka, Haruki Kitazawa, Hirohide Uenishi, Takato Takenouchi

  APVS 2025


• Successful reproduction of microbiota in hysterotomy-produced colostrum-deprived pigs by fecal microbiota transplantation.

  Yuto Suda, Yoshihiro Muneta, Sayaka Nishikawa, Natsumi Kishida, Kanae Hiramatsu, Keigo Ikeda, Yoshifumi Iwamaru, Seiichi Ohashi, Yuki Indo, Fu Namai, Keita Nishiyama, Haruki Kitazawa, Ayako Miyazaki

  APVS 2025


• In vivo Volatile Fatty Acid measurement in the rumen of cattle using polybutadiene-coated quartz microbalance sensor.

  Jarred Fastier-Wooller, Shun Muramatsu, Michitaka Yamamoto, Shintaro Noda, Naoki Shiraishi, Hironao Okada, Yoshihiro Muneta, Shozo Arai, Toshihiro Itoh

  2025 IEEE SENSORS


• Output Power improvement of rumen bacteria microbial fuel cells using NI-plated conductive foam.

  Michitaka Yamamoto, Yasutaka Shimizu, Jarred W Fastier-Wooller, Yoshihiro Muneta, Hiroshi Sawada, Shozo Arai, Toshihiro Itoh

  2025 23rd International Conference on solid-state sensors, actators and microsystems.


• 腸内細菌叢と腸内代謝産物の重要性とその制御に向けた取り組み

  宗田吉広

  令和6年度 東北農業試験研究推進会議 大家畜中小家畜分科会 講演, Jan. 2025


• ルーメン細菌叢発電の発電槽に由来する細菌のシングルセルゲノム解析

  宗田吉広, 小川洋介, 西村祐貴, 山本道貴, 伊藤寿浩, 澤田 浩, 新井鐘蔵

  2024年度先進ゲノム支援拡大班会議

  Poster presentation


• The Development of Rumen Bacteria Microbial Fuel Cell in anaerobic environments

  Yasutaka Shimizu, Jarred Fastier-Wooller, Yoshihiro Muneta, Hiroshi Sawada, Michitaka Yamamoto, Seiichi Takamatsu, Shozo Arai, Toshihiro Itoh

  2024 IEEE SENSORS

  Poster presentation


• 子宮切断術摘出・初乳未接種豚への糞便細菌叢移植実験系の構築

  須田遊人, 生井 楓, 宗田吉広, 西川明芳, 大橋誠一, 岸田なつみ, 橘紅李, 西山啓太, 北澤春樹, 宮崎綾子

  第132回日本畜産学会


• 子宮切断術摘出・初乳未接種豚への糞便細菌叢移植とその腸管バリア機能と免疫学的影響の解析

  須田遊人, 生井 楓, 宗田吉広, 西川明芳, 大橋誠一, 岸田なつみ, 橘紅李, 西山啓太, 北澤春樹, 宮崎綾子

  第167回日本獣医学会


• 豚の腸内細菌叢と腸内代謝産物及びその制御に向けたアプロ―チ

  宗田吉広

  令和6年度日本養豚開業獣医師協会豚病講習講演, Aug. 2024


• 豚健全育成のための新戦略ーイムノバイオティクスと抗病性改良DNAマーカーの活用ー

  宗田吉広

  JRA/BRAIN共催シンポジウム講演, Nov. 2023


• Bovine rumen electricity- a possible future energy for sustainable livestock industry.

  Yoshihiro Muneta

  Federation of Asian Veterinary Associations (FAVA2022)., 2022


• サルモネラ認識を低下させる豚TLR5の一塩基多型とその豚サルモネラ症に及ぼす影響の検証

  宗田吉広

  第161回日本獣医学会・シンポジウム講演, Sep. 2018


• サルモネラ認識を低下させる豚TLR5の一塩基多型とその豚サルモネラ症に及ぼす影響の検証

  宗田吉広

  第10回日本ブラキスピラ学会学術集会・特別講演, Jul. 2018


• Experimental infection of Salmonella enterica serovar Typhimurium in weaned, specific pathogen-free Landrace piglets.

  Yoshihiro Muneta

  6th Joint Japan-Thai NIAH Conference., 2018


• Salmonella enterica serovar Choleraesuis and Typhimurium to in vitro produced porcine macrophages.

  Yoshihiro Muneta

  7th International Symposium on Emerging and Re-emerging Pig Diseases., 2015


• Serum interleukin-18 (IL-18) concentration can be a useful indicator to evaluate hygiene status of pig production farm.

  Yoshihiro Muneta

  Federation of Asian Veterinary Associations (FAVA2014)., 2014


• サルモネラ認識を低下させる豚TLR5の一塩基多型（C1205T）簡易検出法の開発

  宗田吉広

  アグリビジネス創出フェア2013, Oct. 2013


• Experimental infection of Salmonella enterica serovar Choleraesuis to HPCD or weaned piglets; effect of a TLR5 SNP (C1205T).

  Yoshihiro Muneta

  10th International Veterinary Immunology Symposium., 2013


• 家畜マイコプラズマ感染症の宿主免疫応答の特徴

  宗田吉広

  第39回日本マイコプラズマ学会・シンポジウム講演, May 2012


• Lactobacillus casei I-5 induces Il-10 and suppresses stress-induced cytokine Il-18 and changes intra-intestinal conditions in pigs.

  Yoshihiro Muneta

  International Pig Veterinary Society (IPVS2012)., 2012


• Development of allele-specific primer PCR for a swine TLR5 SNP (C1205T; P402L) and comparison of the frequency in pigs between Japan and the Czech Republic.

  Yoshihiro Muneta

  International Union of Microbiological Societies (IUMS2011)., 2011


• Development of allele-specific primer PCR for a swine TLR2 (C406G; P136A) and comparison of the frequency in pigs between Japan and the Czech Republic.

  Yoshihiro Muneta

  5th Asian Organization for Mycoplasmology, 2011


• Salivary IL-18 and IgA are useful non-invasive stress markers in pigs.

  Yoshihiro Muneta

  5th Asian Pig Veterinary Society, 2011


• 豚のストレスマーカーとしてのサイトカインの応用

  宗田 吉広

  第7回動物サイトカイン研究会・シンポジウム講演, Sep. 2010


• Mycoplasma hyopneumniae の宿主認識分子の多型解析と豚マイコプラズマ肺炎制御技術への応用

  宗田吉広, 新開浩樹, 柴田千尋, 上西博英, 皆川 遊, 小川洋介, 芝原友幸, 下地善弘

  第36回日本マイコプラズマ学会・シンポジウム講演, Jun. 2010


• Development of Allele specific primer PCR for a swine TLR2 and TLR5 SNP and comparison of the frequency in pigs between Japan and Czech Republic.

  宗田吉広

  OECDフェローシップ2009成果報告会, May 2010


• 動物サイトカインの利活用による家畜疾病制御技術に関する研究

  宗田吉広

  第8回日本農学進歩賞受賞記念講演, Nov. 2009


• 若手研究者11人を表彰ー農学会

  宗田吉広

  日本農業新聞, Nov. 2009

  Media report


• Single nucleotide polymorphisms in Toll-like receptor 2 affect the susceptibility of Mycoplasma hyopneumoniae infection in pigs.

  Yoshihiro Muneta

  4th Asian Organization for Mycoplasmology, 2009


• Effects of the allele containing a swine TLR2 SNP (C406G→P136A) on Mycoplasma hyopneumoniae gnotobiotic infection.

  Yoshihiro Muneta

  44th USA-Japan National Research for Animal and Avian Health Panel Meeting., 2009


• IL-18 expression in salivary glands and its change in saliva during acute restraint stress in pigs.

  Yoshihiro Muneta

  14th International Congress of Mucosal Immunology, 2009


• The allele containing P136A mutation of porcine toll-like receptor 2 appears to increase susceptibility to mycoplasma pneumonia.

  Yoshihiro Muneta

  11th International Society of Developmental and Comparative Immunology., 2009


• 動物のストレスを非侵襲的に評価する指標の開発を目指して

  宗田吉広, 皆川 遊

  つくばTXテクノロジー・ショーケース2009, Jan. 2009


• 唾液による動物のストレス評価

  宗田吉広

  アグリビジネス創出フェア2008, Oct. 2008


• 豚マイコプラズマ肺炎の発病機構の解明と疾病制御への応用

  宗田吉広, 皆川遊, 小川洋介, 彦野弘一, 下地善弘, 新開浩樹, 上西博英, 森 康行

  第35回日本マイコプラズマ学会・シンポジウム講演, May 2008


• Development and immunogenicity of recombinant Mycoplasma gallisepticum vaccine strain ts-11 expressing chicken IFN-gamma.

  Yoshihiro Muneta

  17th International Organization for Mycoplasmology, 2008


• マイコプラズマ肺炎の発病機構の解明と粘膜ワクチン開発への応用

  宗田吉広, Philip Markham, Glenn Browning

  第5回動物サイトカイン研究会・特別講演, Sep. 2007


• IL-18 expression in Mycoplasma hyopneumoniae infection in pigs.

  Yoshihirio Muneta

  7th International Veterinary Immunology Symposium, 2004


• 豚インターロイキン18に関する研究

  宗田吉広

  動物サイトカイン研究会設立総会・シンポジウム講演, Apr. 2003


• Porcine Interleukin-18 and its converting enzyme; cloning, expression, and their roles in inflammation.

  Yoshihiro Muneta

  36th USA-Japan National Research for Animal and Avian Health Panel Meeting, 2002


• 豚のインターロイキン18とその変換酵素に関する研究

  宗田吉広

  第565回北海道大学獣医学部談話会, 2002


• Expression of IL-18 in pig airway and intestinal epithelium.

  Yoshihiro Muneta

  6th International Veterinary Immunology Symposium, 2001


• Production and utilization of pig cytokines using baculovirus-insect expression systems.

  Yoshihiro Muneta

  6th NISES/COE International Symposium, 2001


• 豚のインターロイキン18

  宗田 吉広

  第9回動物エンドトキシン研究会・教育講演, 2001


• Porcine Interleukin-18 and its converting enzyme; cloning, characterization and biological activity.

  Yoshihiro Muneta

  International Veterinary Cytokine and Vaccine Conference, 2000


• Mplecular cloning of porcine Fas-ligand and caspase-3; their relationship with apoptosis of porcine PK-15 cells.

  Yoshihiro Muneta

  3rd Joint meeting of the International Cytokine Society / International Society of Interferon and Cytokine Research, 2000


• Molecular cloning of porcine interleukin-18, interleukin-1beta converting enzyme and their mRNA expression in porcine alveolar macrophages

  Yoshihiro Muneta

  7th Annual Conference of International Cytokine Society, 1999

• 日本豚病研究会


• 日本マイコプラズマ学会


• 動物用ワクチン・バイオ医薬品研究会


• 日本インターフェロン・サイトカイン学会


• 日本獣医学会


• 日本獣医師会


• 日本畜産学会

• 薬剤削減のための豚腸・肺免疫連関実証事業

  JRA畜産振興事業, Apr. 2024 - Mar. 2027, Coinvestigator


• ルーメン細菌発電デバイスの開発

  伊藤 寿浩, 宗田 吉広

  日本学術振興会, 科学研究費助成事業, 基盤研究(B), 東京大学, 01 Apr. 2023 - 31 Mar. 2026

  本研究は、畜産動物のルーメン内環境をモニタリングするためのルーメンセンサ用の自立電源デバイスとして、ルーメン内の細菌による発電デバイスを開発することを目的としている。具体的には、ルーメン中で継続的に発電ができ、その平均発電電力が、少なくとも１時間に１回程度の無線送信と連続的なセンシングを可能とする1 mW以上となるデバイスの開発を目指す。そのために発電細菌の同定と動態解明を行うとともに、発電細菌の特性を最大限に引き出すことのできる電極構造の開発を行う。 本年度は、上記目的のため、１）発電細菌候補の絞り込み、２） 電極寿命改善のためのCNT電極の開発、３）ルーメン環境下での発電のための細菌の分離・固定化手法の検討 を行った。 １）については、長期間にわたり良好な発電を継続しているルーメン細菌叢発電の電極として使用している竹炭電極（陽極及び陰極）について、シングルセルゲノム解析を行い、細菌属のゲノム、および細胞外への電子伝達経路に関与する遺伝子群等を保有していることも確認した。 ２）については、これまで良好な発電が行えていたCuペーパー電極に代えて、CNTペーパー電極を検討し、Cuペーパー電極と同等の発電量を維持しながら、電極寿命を2週間程度から半年以上に改善することに成功した。 実際のルーメン環境下で発電を持続させるためには、陽極・陰極それぞれに異なる細菌を分離・定着させる必要がある。そこで３）については、電極表面にPEDOT:PSSによって細菌を固定化する方法、および、陽極・陰極のチャンバーを分けることで細菌を分離する方法の検討を行った。


• イムノシンバイオティクスとDNAマーカーによる豚の腸内環境改善を介する抗病性向上手法の開発事業

  JRA畜産振興事業, Apr. 2021 - Mar. 2024, Coinvestigator


• 内在性抗菌因子PGRPに着目した育種と飼養の抗病性シナジー強化による健全畜産戦略

  北澤 春樹, 大坪 和香子, 麻生 久, 野地 智法, 上西 博英, 宮崎 綾子, 宗田 吉広, 須田 義人

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A), Grant-in-Aid for Scientific Research (A), Tohoku University, Apr. 2019 - Mar. 2023

  本年度は、以下の成果が得られた。 【課題１】子豚のPGRP2・3・4において、プロモータ及びコード領域でSNPを検出した。それらからハプロタイプの再構成を試み、PGRP2で3種、PGRP3及びPGRP4でそれぞれ7種を検出した。PGRPの遺伝型と発現の関連性を検討したところ、PGRP4の特定のハプロタイプを持つ個体について、消化器系各臓器において処置内容に関わらずPGRP4が高発現する傾向が確認された。【課題２】我々が保有するブタ由来乳酸桿菌ライブラリーより、昨年度までに評価をした菌種に加え、新たな菌種についても、ブタ小腸上皮細胞を用いてPGRPの遺伝子発現を増強するイムノバイオティクス候補菌株の評価を行った。昨年までと同様に、発現調節能は菌株特異的であり、内在性抗菌因子の強化に有用なイムノバイオティクス候補菌株の存在が再認識でき、その探索を進めることができた。【課題３】イムノバイオティクス候補菌株を7日間連続経口投与した子宮切断術作出初乳未接種（HPCD）子豚に豚ロタウイルスを経口投与した結果、ウイルス接種後3日目及び5日目の糞便中ウイルス排泄量が菌株によって対照のPBS投与群よりも有意に低かった。また、HPCD子豚末梢血中貪食細胞の化学蛍光（CL）活性は菌株投与後、FFIG67株においてはウイルス接種日あるいは接種後7日目にかけてPBS群と比較して顕著な増加が確認された。【課題４】イムノバイオティクス候補菌株を抗菌剤無添加飼料給与LWD交雑豚に投与したところ、対照区に比べ下痢の頻度が抑えられ、毒素原性大腸菌の感染は有意に低かった。さらに豚肉における総不飽和脂肪酸組成割合が有意に高くなり、枝肉格付け評価も高まった。以上のことから、イムノバイオティクス候補菌株の投与は、健康性を保ちながら産肉性の向上に寄与することが期待された。


• Development of an Ultra-Long-Life Miniature Rumen pH Sensor Using a Microfluidic Control Device

  Itoh Toshihiro

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), The University of Tokyo, Apr. 2019 - Mar. 2022

  In order to realize an ultra-long-life miniature rumen pH sensor, we realized an intermittently operating reference electrode by introducing a low power SMA valve-actuator into a liquid junction and developing a new Ag/AgCl electrode device that can redeposit AgCl. We also proposed a planar all-solid-state glass electrode structure with pressure-sensitive adhesive for ultra-long-life pH sensors, and fabricated a wireless rumen pH sensor with the prototyped glass electrode, which was fed into a fistula-forming cow for wireless communication demonstration. In addition, we investigated the electrode and mounting structure of a rumen bacterial flora power generator to introduce it into the wireless rumen pH sensor as a self-power generator.


• 豚の抗病性改良DNAマーカー実証事業

  JRA畜産振興事業, Apr. 2018 - Mar. 2021

• 陽極及び陰極にルーメン内微生物（細菌叢）を定着させた竹炭電極を用いた発電方法

  宗田吉広, 澤田 浩, 芝原友幸, 新井鍾蔵

  特願2021-140074, 30 Aug. 2021, 農研機構, 特許第7208693号, 11 Jan. 2023

  Patent right

  IRI


• サイトカインを発現する豚丹毒菌及び豚丹毒菌を用いたサイトカインのデリバリー方法

  下地善弘, 宗田吉広, 小川洋介

  特願2010-179356, 特許第5991650号, 26 Aug. 2016

  Patent right


• 豚丹毒・豚マイコプラズマ肺炎経口投与型多価ワクチン

  下地善弘, 宗田吉広, 小川洋介, 大石英司

  特願2009-40970, 特許第5257939号, 02 May 2013

  Patent right


• 抗豚インターロイキン-18抗体を用いた動物のストレス評価方法

  宗田 吉広

  特許5219048, 15 Mar. 2012

  Patent right


• 昆虫細胞又は昆虫を用いた活性型ブタIL-18の生産方法

  宗田吉広, 森 康行, 下地善弘, 新井啓五

  特許第3541216号, 09 Apr. 2004, 特許第3541216号

  Patent right


• ブタ由来インターロイキン18に対するモノクローナル抗体

  宗田吉広, 森 康行, 下地善弘, 新井啓五

  特許第3230220号, 14 Sep. 2001, 特許第3230220号

  Patent right


• ヨーネ菌抗原タンパク質、該タンパク質をコードする遺伝子、及び該タンパク質を用いるヨーネ病の診断法

  森 康行, 永田礼子, 吉原一浩, 宗田吉広, 横溝祐一

  特願2003-334977, 特許第3864230号

  Patent right


• Oral polyvalent vaccine against swine erysipelas / mycoplasmal pneumonia

  Shimoji Yoshihiro, Muneta Yoshihiro, Ogawa Yohsuke, Oishi Eiji

  特願PCT/JP2009/065502

  Patent right