Research activity information

• Oct. 2008 消化器病学会, 優秀ポスター賞, 当院で経験した膵管内乳頭粘液性腫瘍(IPMN)の他臓器癌の合併についての検討

  Inoue Jun

  Japan society

• Construction of a Model Culture System of Human Colonic Microbiota to Detect Decreased Lachnospiraceae Abundance and Butyrogenesis in the Feces of Ulcerative Colitis Patients

  Sasaki K, Inoue Jun, Sasaki D, Hoshi Namiko, Shirai T, Fukuda I, Azuma T, Kondo A, Osawa R

  Feb. 2019, Biotechnol J, English

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Construction of a model culture system of human colonic microbiota to detect decreased Lachnospiraceae abundance and butyrogenesis in the feces of ulcerative colitis patients

  SASAKI KENGO, INOUE JUN, SASAKI DAISUKE, HOSHI NAMIKO, SHIRAI TOMOKAZU, FUKUDA ITSUKO, AZUMA TAKESHI, KONDO AKIHIKO, OSAWA RO

  Compositional alteration of the gut microbiota is associated with ulcerative colitis (UC). Here, a model culture system is established for the in vitro human colonic microbiota of UC, which will be helpful for determining medical interventions. 16S ribosomal RNA sequencing confirms that UC models are successfully developed from fecal inoculum and retain the bacterial species biodiversity of UC feces. The UC models closely reproduce the microbial components and successfully preserve distinct clusters from the healthy subjects (HS), as observed in the feces. The relative abundance of bacteria belonging to the family Lachnospiraceae significantly decreases in the UC models compared to that in HS, as observed in the feces. The system detects significantly lower butyrogenesis in the UC models than that in HS, correlating with the decreased abundance of Lachnospiraceae. Interestingly, the relative abundance of Lachnospiraceae does not correlate with disease activity (defined as partial Mayo score), suggesting that Lachnospiraceae persists in UC patients at a decreased level, irrespective of the alteration in disease activity. Moreover, the system shows that administration of Clostridium butyricum MIYAIRI restores butyrogenesis in the UC model. Hence, the model detects deregulation in the intestinal environment in UC patients and may be useful for simulating the effect of probiotics.

  2019, Biotechnology Journal, 14(5) (5), e1800555, English, International magazine

  [Refereed]

  Scientific journal

  Link to Kobe Univ. Repository (Kernel)


• Proton Pump Inhibitors Increase the Susceptibility of Mice to Oral Infection with Enteropathogenic Bacteria.

  Eiichiro Yasutomi, Namiko Hoshi, Soichiro Adachi, Takafumi Otsuka, Lingling Kong, Yuna Ku, Haruka Yamairi, Jun Inoue, Tsukasa Ishida, Daisuke Watanabe, Makoto Ooi, Masaru Yoshida, Tomoya Tsukimi, Shinji Fukuda, Takeshi Azuma

  BACKGROUND AND AIMS: Proton pump inhibitors (PPIs) are among the most frequently prescribed medications. Side effects including an increased risk of intestinal infections have been reported. It is assumed that PPIs can increase susceptibility to enteropathogens; however, the underlying mechanisms are unknown. Here in this study, we explored whether Lansoprazole (Laz), one of the PPIs, increases the susceptibility to enteropathogens, and further investigated the mechanism of it. METHODS: Mice were administered Laz intraperitoneally once daily and orally infected with Citrobacter rodentium (C. rodentium). The establishment of intestinal infection was assessed by histology and inflammatory cytokine expression levels measured by quantitative PCR. To test whether Laz changes the intestinal environment to influence the susceptibility, intestinal pH, microbiota, metabolites and immune cell distributions were evaluated via pH measurement, 16S rRNA gene sequencing, metabolome, and flow cytometry analyses after Laz administration. RESULTS: Colitis was induced with less C. rodentium in Laz-treated mice as compared with the controls. We found that increased numbers of C. rodentium could reach the cecum following Laz administration. Laz increased pH in the stomach but not in the intestines. It induced dysbiosis and changed the metabolite content of the small intestine. However, these changes did not lead to alterations of immune cell distribution. CONCLUSIONS: Laz raised susceptibility to C. rodentium as increased numbers of the pathogen reach the site of infection. Our results suggest that it was due to increased stomach pH which allowed more peroral enteropathogens to pass the stomach, but not because of changes of intestinal environment.

  Apr. 2018, Digestive diseases and sciences, 63(4) (4), 881 - 889, English, International magazine

  [Refereed]

  Scientific journal


• Cisplatin resistance in human lung cancer cells is linked with dysregulation of cell cycle associated proteins

  Sayo Horibe, Akira Matsuda, Toshihito Tanahashi, Jun Inoue, Shoji Kawauchi, Shigeto Mizuno, Masaki Ueno, Kyohei Takahashi, Yusaku Maeda, Tatsuya Maegouchi, Yoshiki Murakami, Ryoko Yumoto, Junya Nagai, Mikihisa Takano

  Mar. 2015, LIFE SCIENCES, 124, 31 - 40, English

  [Refereed]

  Scientific journal


• Intestinal and hepatic expression of cytochrome P450s and mdr1a in rats with indomethacin-induced small intestinal ulcers

  KawauchiS, NakamuraT, YasuiH, NishikawaC, MikiI, Inoue Jun, HoribeS, HamaguchiT, TanahashiT, MizunoS

  Sep. 2014, Int J Med Sci, 11(12) (12), 1208 - 17, English

  [Refereed]

  Scientific journal


• Downregulation of CYP3A and P-glycoprotein in the secondary inflammatory response of mice with dextran sulfate sodium-induced colitis and its contribution to cyclosporine A blood concentrations

  KawauchiS, NakamuraT, MikiI, Inoue Jun, HamaguchiT, TanahashiT, MizunoS

  2014, J Pharmacol Sci, 124(2) (2), 180 - 91, English

  [Refereed]

  Scientific journal


• Mitochondria determine the efficacy of anticancer agents that interact with DNA but not the cytoskeleton

  HaraK, KasaharaE, TakahashiN, KonishiM, Inoue Jun, JikumaruM, KuboS, OkamuraH, SatoE, InoueM

  Jun. 2011, J Pharmacol Exp Ther, 337(3) (3), 838 - 45, English

  [Refereed]

  Scientific journal


• 膵切除後に残膵に再燃をきたした自己免疫性膵炎の2例

  Inoue Jun, MasudaA, SaitoM, OnoyamaM, ShiomiH, ToyamaH, ShinzekiR, MatsumotoI, HayashiY, MakinoT, TadaH, KutsumiH, KuY, AzumaT

  Since the revision of Clinical Diagnostic Criteria for Autoimmune Pancreatitis (AIP) 2006, many cases of localized AIP have been reported. Localized AIP is often difficult to preoperatively differentiate from pancreatic carcinoma. We present two cases of localized AIP that developing relapse after surgical treatment. Swollen hilar lymph nodes of lung was detected on CT in both two cases. Recently, AIP is thought to be the pancreatic manifestation of an IgG4 related systemic disease, which has been associated with many extrapancreatic lesions. Response to steroid treatment and the detection of extrapancreatic lesions may contribute to provide adequate diagnosis thereby avoiding unnecessary surgery.
  

  The Japanese Society of Gastroenterology, 2011, 日本消化器病学会誌, 108(4) (4), 640 - 649, Japanese

  [Refereed]

  Scientific journal


• Oxidative stress and renal pathophysiology.

  KasaharaE, Inoue Jun, JikumaruM, SatoE, InoueM

  2011, Rinsho Byori, 48(6) (6), 187 - 188, Japanese

  [Refereed]

  Scientific journal


• Ultraviolet-A irradiation to the eye modulates intestinal mucosal functions and properties of mast cells in the mouse

  YamateY, HiramotoK, KasaharaE, JikumaruM, SatoEF, Inoue Jun, InoueM

  Jan. 2011, Photochem Photobiol, 87(1) (1), 191 - 8, English

  [Refereed]

  Scientific journal


• Role of hydrogen generation by Klebsiella pneumoniae in the oral cavity

  KanazuruT, SatoEF, NagataK, MatsuiH, WatanabeK, KasaharaE, JikumaruM, Inoue Jun, InoueM

  Dec. 2010, J Microbiol, 48(6) (6), 778 - 83, English

  [Refereed]

  Scientific journal

• 腎と透析 / 腸内細菌叢と尿毒症物質

  Inoue Jun, 星奈美子, 児玉裕三

  Others, 東京医学社, 2018, Japanese

  Scholarly book


• メタゲノム解析技術の最前線 / 疾患とメタゲノム

  山本幸司, 吉田優, Inoue Jun, 大井充, 吉江智郎, 東健

  Others, シーエムシー, 2010, Japanese

  Scholarly book

• In vitro培養系ヒト腸内細菌叢モデルによる潰瘍性大腸炎患者の代謝プロファイル異常の検出

  SASAKI KENGO, INOUE JUN, HOSHI NAMIKO, SASAKI DAISUKE, FUKUDA ITSUKO, KONDO AKIHIKO, OSAWA RO

  第70回日本生物工学会大会, Sep. 2018, Japanese, 関西大学 千里山キャンパス, Domestic conference

  Poster presentation


• Successful detection of dysbiosis and altered short-chain-fatty acids levels in in vitro colonic microbiota culture system using faecal samples of ulcerative colitis

  Inoue Jun, 佐々木 建吾, Hoshi Namiko, 佐々木 大介, 近藤 昭彦, 大澤 朗

  European Crohn's and Colitis Organisation, Feb. 2018, English, オーストリア, ウィーン, International conference

  Poster presentation


• 複数の腸炎モデルマウスに対する漢方薬青黛の効果の検討

  足立 聡一郎, Hoshi Namiko, Inoue Jun, 安冨 栄一郎, 大塚 崇史, Ramesh Dhakhwa, 王 梓, 孔 玲玲, 渡邉 大輔, Ooi Makoto, Yoshida Masaru

  The 8th Annual Meeting of Japanese Society for Inflammatory Bowel disease, Dec. 2017, Japanese, Japanese Society for Inflammatory Boewel Disease, 東京, Domestic conference

  Poster presentation


• Increased susceptibility to enteropathogenic bacteria by proton pump inhibitors in the murine model of food poisoning

  安冨 栄一郎, Inoue Jun, 足立 聡一郎, 大塚 崇史, 渡邉 大輔, 具 潤亜, 山入 春香, Ooi Makoto, Hoshi Namiko, 福田 真嗣, Yoshida Masaru, Azuma Takeshi

  25th United European Gastroenterology Week, Oct. 2017, English, United European Gastroenterology, バルセロナ, スペイン, International conference

  Poster presentation

• 過敏性腸症候群における腸管粘膜上皮表層細菌叢と病勢との相関解析

  井上 潤

  学術研究助成基金助成金／若手研究(B), Apr. 2017 - Mar. 2019, Principal investigator

  Competitive research funding